[Histonet] RE: holes in nuclei of FFPE tissue after IHC staining

[Subash Govender]

Dear All
I am having a problem with my immunohstochemistry staining.  The nuclei of the cells have holes in the middle.  I have a feeling that it could be my pressure cooking procedure. I pressure cook the slides for 1min 30 secs and leave them to stand for 20 mins thereafter.  This is strange because i did not always have this problem.  The cells seem to be overcooked. This occurs with both EDTA  pH 8,9 and citric acid pH6 solutions.  Has anybody had this problem or can anyone help please.

Thank you very much.

Subash Govender

Medical Technologist
Anatomical Pathology Research Lab
Medical School
University of Cape Town
South Africa
Tel: +27 21 4066599
email: subash.govender <@t> uct.ac.za

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From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] on behalf of histonet-request <@t> lists.utsouthwestern.edu [histonet-request <@t> lists.utsouthwestern.edu]
Sent: 04 September 2013 07:01 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 118, Issue 4

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Today's Topics:

   1. RE: This is not Facebook (hymclab)


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Message: 1
Date: Wed, 4 Sep 2013 11:54:38 -0500
From: hymclab <hymclab.hymclab <@t> ministryhealth.org>
Subject: RE: [Histonet] This is not Facebook
To: "'Marsh, Nannette'" <NMP <@t> stowers.org>, ihcs-wojcieszyn
        <ihcs <@t> smithsys.net>, "Histonet <@t> lists.utsouthwestern.edu"
        <Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <EF3001233998854390C692D19B058F0F71046054DB <@t> EXMHCMBX01VS.ministryhealth.net>

Content-Type: text/plain; charset="us-ascii"

I responded to jw privately but want to show my support for Hazel or anyone else that is facing a CAP inspection.  I think Hazel and everyone that posts that CAP is in their institution is looking for moral support and I sent Hazel a message right away saying "Good luck".  I hope Hazel or anyone else posts how their inspection goes and lets the rest of us know any issues we should be on the look out for!~!!!!

We all know how trying it can be to be inspected!!!!  Just hit delete if you don't want to be friedly and show your support!!!


Dawn D. Schneider, HT(ASCP)
Lead HT
Howard Young Medical Center
240 Maple Ave.
Woodruff, WI 54558
715-356-8174

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Marsh, Nannette
Sent: Wednesday, September 04, 2013 8:48 AM
To: ihcs-wojcieszyn; Histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] This is not Facebook

Wow, life is too short.  We don't have to take things so seriously all of the time.  I do hope your day gets better :-)

Nanne Marsh HT ASCP

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of ihcs-wojcieszyn
Sent: Tuesday, September 03, 2013 8:36 PM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] This is not Facebook

E-mails that tell all of what is happening, i.e. "CAP is in house" is a waste of time. Are we going to communicate when you wash your hands or have a cup of coffee.
If Histonet is to be a source of information, ASK a question


jw


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End of Histonet Digest, Vol 118, Issue 4
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