From tahseen <@t> brain.net.pk Sun Sep 1 12:55:20 2013 From: tahseen <@t> brain.net.pk (tahseen) Date: Sun Sep 1 12:55:32 2013 Subject: [Histonet] Tissue Processor Validation In-Reply-To: <25A4DE08332B19499904459F00AAACB719C704FD1D@EVS1.archildrens.org> References: <3AD061FE740D464FAC7BF6B5CFB75707A8A2A7EB@smcmail02.somerset-healthcare.com> <25A4DE08332B19499904459F00AAACB719C704FD1D@EVS1.archildrens.org> Message-ID: Dear All Would any one of you out there,want to share template of Tissue Processor validation forms? Thanks Muhammad Tahseen Histolog Supervisor SKMCH&RC Lahore Pakistan On 2013-08-28 19:10, Horn, Hazel V wrote: > We did the same as Beth. > > Hazel Horn > ySupervisor of Histolog/Autopsy/Transcription > Anatomic Pathology > Arkansas Children's Hospital > 1 Children's Way | Slot 820| Little Rock, AR 72202 > 501.364.4240 direct | 501.364.1241 fax > hornhv@archildrens.org > archildrens.org > > > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Beth > Brinegar > Sent: Wednesday, August 28, 2013 8:40 AM > To: Rathborne, Toni > Cc: histonet@lists.utsouthwestern.edu; DianaRip1@aol.com > Subject: Re: [Histonet] Tissue Processor Validation > > Yes, we do a few validation runs. Nothing too extreme, however we > gross some extra patient tissue and do a small run, then increase the > amount of blocks processed until we are ready to run cases for > patient > diagnosis. It really only takes a week or two. After those blocks are > cut, then we compare them to the previous processor results and make > changes if neccessary. > > Beth Brinegar HTL(ASCP) > Anatomic Pathology Supervisor > Mercy Medical Center > Cedar Rapids, IA 52403 > > > On Wed, Aug 28, 2013 at 8:27 AM, Rathborne, Toni < > trathborne@somerset-healthcare.com> wrote: > >> How do you validate IHC & FISH with a new processor? >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu [mailto: >> histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelle >> weaver >> Sent: Wednesday, August 28, 2013 9:23 AM >> To: DianaRip1@aol.com; histonet@lists.utsouthwestern.edu >> Subject: RE: [Histonet] Tissue Processor Validation >> >> Yes definitely, 5 programs, 25-50 sister, serial sections on tissue >> that represents patient samples. >> >> >> >> >> Joelle Weaver MAOM, HTL (ASCP) QIHC >> >> > From: DianaRip1@aol.com >> > Date: Tue, 27 Aug 2013 22:07:19 -0400 >> > To: histonet@lists.utsouthwestern.edu >> > Subject: [Histonet] Tissue Processor Validation >> > >> > I am interested in knowing how many of you out there Validate your >> > Tissue Processors before you put them into use and how extreme you >> go. >> > >> > Diana Ripley >> > _______________________________________________ >> > Histonet mailing list >> > Histonet@lists.utsouthwestern.edu >> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** > The information contained in this message may be privileged and > confidential > and protected from disclosure. If the reader of this message is not > the > intended recipient, or an employee or agent responsible for > delivering this > message to the intended recipient, you are hereby notified that any > dissemination, distribution or copying of this communication is > strictly > prohibited. If you have received this communication in error, please > notify > us immediately by replying to the message and deleting it from your > computer. > Thank you. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From a.thotakura <@t> imperial.ac.uk Mon Sep 2 11:09:06 2013 From: a.thotakura <@t> imperial.ac.uk (Thotakura, Anil K) Date: Mon Sep 2 11:09:17 2013 Subject: [Histonet] PHD3 and DC-SIGN/CD209 In-Reply-To: <2FE9E70AEAB06A46977ACFEE6C22B31E23817B94@icexch-m4.ic.ac.uk> Message-ID: <2FE9E70AEAB06A46977ACFEE6C22B31E2AC7D954@icexch-m4.ic.ac.uk> Dear All, Could some one help me find these (PHD3 and DC-SIGN/CD209) antibodies that work for mouse frozen tissues. I was search for a reference entire last week but no success. Could any one help me with this??? Thank you very much for your help. Many Thanks, Anil From: , "Thotakura, Anil K" > Date: Thursday, 22 August 2013 19:06 To: "Histonet@lists.utsouthwestern.edu" > Subject: Gadd45beta Hi All, Did any tried Gadd45beta antibody for IHC on mouse paraffin tissue samples???? Please let me know, I am desperate for a protocol. Many Thanks, Anil From Ken_Marissael <@t> vwr.com Mon Sep 2 12:04:05 2013 From: Ken_Marissael <@t> vwr.com (Ken_Marissael@vwr.com) Date: Mon Sep 2 12:04:15 2013 Subject: [Histonet] Ken Marissael is out of the office Message-ID: I will be out of the office starting 08/31/2013 and will not return until 09/10/2013. I will be away until September 10, 2013. During my absense Kerri O'Brien will be covering my territory. She can be reached via e-mail (kerri_obrien@vwr.com or by cell (551-497-0848) If you have an emergency, please contact - healthcareservice.com or customer service at 877-881-1192. From lcolbert <@t> pathmdlabs.com Tue Sep 3 09:16:13 2013 From: lcolbert <@t> pathmdlabs.com (Laurie Colbert) Date: Tue Sep 3 09:19:05 2013 Subject: [Histonet] Razor Blade Holder Message-ID: <12ECD7346266D74691EC2BFC75285E452F339F42@BFL323E10.pathmdlabs.local> Does anyone know where I can purchase the little plastic holder for razor blades for trimming your blocks and what the thing is called?? Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab From Susan.Wert <@t> cchmc.org Tue Sep 3 10:12:38 2013 From: Susan.Wert <@t> cchmc.org (Wert, Susan (Susan Wert)) Date: Tue Sep 3 10:12:44 2013 Subject: [Histonet] Goat anti-Rabbit Alexa 594 Message-ID: <12F351B8515AD341B90AC3D5D7480C207D9CF63F@MCEXMB2.chmccorp.cchmc.org> Gayle, would you please let us know how long you spun the antibody and at what speed? Thanks. Susan Wert, PhD Assoc. Prof. Cincinnati Children's Hosp. Med. Ctr./Research Found. Morphology Core ? Pulmonary Biology Cincinnati, Ohio USA From MMargiotta <@t> bmhmc.org Tue Sep 3 10:36:23 2013 From: MMargiotta <@t> bmhmc.org (Margiotta-Watz, Michele) Date: Tue Sep 3 10:36:29 2013 Subject: [Histonet] cost of slide Message-ID: <230D0B9EC57D7A45A7A186C6AB4C7ABC29710DC6@BMH-EXCHANGE-01.BMHMC.ORG> Hi All, My pathologist wants to know how much other labs are charging for recutting an H&E slide to send out to a law firm for example. I know this has been discussed before on Histonet but I couldn't find any info. Your help is appreciated! Thanks, Michele M. Histology Supervisor BMHMC DISCLAIMER: This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. From DKBoyd <@t> chs.net Tue Sep 3 10:38:14 2013 From: DKBoyd <@t> chs.net (Boyd, Debbie M) Date: Tue Sep 3 10:38:25 2013 Subject: [Histonet] RE: cost of slide In-Reply-To: <230D0B9EC57D7A45A7A186C6AB4C7ABC29710DC6@BMH-EXCHANGE-01.BMHMC.ORG> References: <230D0B9EC57D7A45A7A186C6AB4C7ABC29710DC6@BMH-EXCHANGE-01.BMHMC.ORG> Message-ID: <7EAFE982E328304DA6CE2B677BB76246794F6948@TN001WEXMBX12.US.chs.net> Per recut slide $50.00. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Margiotta-Watz, Michele Sent: Tuesday, September 03, 2013 11:36 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] cost of slide Hi All, My pathologist wants to know how much other labs are charging for recutting an H&E slide to send out to a law firm for example. I know this has been discussed before on Histonet but I couldn't find any info. Your help is appreciated! Thanks, Michele M. Histology Supervisor BMHMC DISCLAIMER: This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From rjbuesa <@t> yahoo.com Tue Sep 3 10:45:59 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Sep 3 10:46:03 2013 Subject: [Histonet] cost of slide In-Reply-To: <230D0B9EC57D7A45A7A186C6AB4C7ABC29710DC6@BMH-EXCHANGE-01.BMHMC.ORG> References: <230D0B9EC57D7A45A7A186C6AB4C7ABC29710DC6@BMH-EXCHANGE-01.BMHMC.ORG> Message-ID: <1378223159.34494.YahooMailNeo@web163106.mail.bf1.yahoo.com> Please visit http://www.histosearch.com/rene.html and you will find an article dealing with costs in histology. You will have to work the numbers and increase material costs to current values and adjust for your salary base. Ren? J. ________________________________ From: "Margiotta-Watz, Michele" To: "'histonet@lists.utsouthwestern.edu'" Sent: Tuesday, September 3, 2013 11:36 AM Subject: [Histonet] cost of slide Hi All, My pathologist wants to know how much other labs are charging for recutting an H&E slide to send out to a law firm for example.? I know this has been discussed before on Histonet but I couldn't find any info. Your help is appreciated! Thanks, Michele M. Histology Supervisor BMHMC DISCLAIMER: This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed.? This communication may contain material protected by the attorney-client privilege.? If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited.? If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From chapcl <@t> yahoo.com Tue Sep 3 10:57:33 2013 From: chapcl <@t> yahoo.com (William Chappell) Date: Tue Sep 3 10:57:49 2013 Subject: [Histonet] Histologist Available for Southern California In-Reply-To: <1378223159.34494.YahooMailNeo@web163106.mail.bf1.yahoo.com> References: <230D0B9EC57D7A45A7A186C6AB4C7ABC29710DC6@BMH-EXCHANGE-01.BMHMC.ORG> <1378223159.34494.YahooMailNeo@web163106.mail.bf1.yahoo.com> Message-ID: <024740D2-A502-45CE-BE1E-133E38821C32@yahoo.com> Greetings Histonet, I am writing this for a colleague of mine who is not on Histonet. I have a friend who is relocating from Seattle, Washington. He is an experienced HTL with extensive IHC experience (QIHC). He has over 3 years of Anatomic Pathology Management experience, but would jump at the chance for a bench position. He is looking for anything in San Diego, Riverside, Los Angeles, or San Bernardino county. If anybody, including temp recruiters, has anything, please contact me and I will forward it to my friend. Thanks, William Chappell, HTL(ASCP), QIHC From Valerie.Hannen <@t> parrishmed.com Tue Sep 3 11:56:18 2013 From: Valerie.Hannen <@t> parrishmed.com (Hannen, Valerie) Date: Tue Sep 3 11:56:25 2013 Subject: [Histonet] RE: Razor Blade Holder In-Reply-To: <12ECD7346266D74691EC2BFC75285E452F339F42@BFL323E10.pathmdlabs.local> Message-ID: <450B7A81EDA0C54E97C53D60F00776C3232B4BB647@isexstore03> They are called Mini Scrapers. I have some that are from Mercedes Medical. There are 4/pk. Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.hannen@parrishmed.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Tuesday, September 03, 2013 10:16 AM To: Histonet Post (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Razor Blade Holder Does anyone know where I can purchase the little plastic holder for razor blades for trimming your blocks and what the thing is called?? Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet =================== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" =================== From histowa13 <@t> hotmail.com Tue Sep 3 12:04:55 2013 From: histowa13 <@t> hotmail.com (Debbie Nannenga) Date: Tue Sep 3 12:05:04 2013 Subject: [Histonet] Razor blade holders In-Reply-To: References: Message-ID: You can buy hem in paint supply departments, for example Walmart or any local hardware store Sent from my iPad On Sep 3, 2013, at 10:01 AM, histonet-request@lists.utsouthwestern.edu wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Ken Marissael is out of the office (Ken_Marissael@vwr.com) > 2. Razor Blade Holder (Laurie Colbert) > 3. Goat anti-Rabbit Alexa 594 (Wert, Susan (Susan Wert)) > 4. cost of slide (Margiotta-Watz, Michele) > 5. RE: cost of slide (Boyd, Debbie M) > 6. Re: cost of slide (Rene J Buesa) > 7. Histologist Available for Southern California (William Chappell) > 8. RE: Razor Blade Holder (Hannen, Valerie) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Mon, 2 Sep 2013 13:04:05 -0400 > From: Ken_Marissael@vwr.com > Subject: [Histonet] Ken Marissael is out of the office > To: histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset=US-ASCII > > > I will be out of the office starting 08/31/2013 and will not return until > 09/10/2013. > > I will be away until September 10, 2013. > During my absense Kerri O'Brien will be covering my territory. She can be > reached via e-mail (kerri_obrien@vwr.com or by cell (551-497-0848) > If you have an emergency, please contact - healthcareservice.com or > customer service at 877-881-1192. > > > > > ------------------------------ > > Message: 2 > Date: Tue, 3 Sep 2013 14:16:13 +0000 > From: Laurie Colbert > Subject: [Histonet] Razor Blade Holder > To: "Histonet Post (histonet@lists.utsouthwestern.edu)" > > Message-ID: > <12ECD7346266D74691EC2BFC75285E452F339F42@BFL323E10.pathmdlabs.local> > Content-Type: text/plain; charset="us-ascii" > > Does anyone know where I can purchase the little plastic holder for razor blades for trimming your blocks and what the thing is called?? > > Laurie Colbert, HT (ASCP) > Histology Supervisor > PATH MD > 8158 Beverly Blvd. > Los Angeles, CA 90048 > (323) 648-3214 direct > (424) 245-7284 main lab > > > > ------------------------------ > > Message: 3 > Date: Tue, 3 Sep 2013 15:12:38 +0000 > From: "Wert, Susan (Susan Wert)" > Subject: [Histonet] Goat anti-Rabbit Alexa 594 > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > <12F351B8515AD341B90AC3D5D7480C207D9CF63F@MCEXMB2.chmccorp.cchmc.org> > Content-Type: text/plain; charset="Windows-1252" > > Gayle, would you please let us know how long you spun the antibody and at what speed? Thanks. > > Susan Wert, PhD > Assoc. Prof. > Cincinnati Children's Hosp. Med. Ctr./Research Found. > Morphology Core ? Pulmonary Biology > Cincinnati, Ohio USA > > > ------------------------------ > > Message: 4 > Date: Tue, 3 Sep 2013 15:36:23 +0000 > From: "Margiotta-Watz, Michele" > Subject: [Histonet] cost of slide > To: "'histonet@lists.utsouthwestern.edu'" > > Message-ID: > <230D0B9EC57D7A45A7A186C6AB4C7ABC29710DC6@BMH-EXCHANGE-01.BMHMC.ORG> > Content-Type: text/plain; charset="us-ascii" > > Hi All, > My pathologist wants to know how much other labs are charging for recutting an H&E slide to send out to a law firm for example. I know this has been discussed before on Histonet but I couldn't find any info. Your help is appreciated! > > Thanks, > Michele M. > Histology Supervisor > BMHMC > DISCLAIMER: > > This e-mail and any files transmitted with it are confidential and are > intended solely for the use of the individual or entity to which they > are addressed. This communication may contain material protected by > the attorney-client privilege. If you are not the intended recipient > or the person responsible for delivering the e-mail to the intended > recipient, be advised that you have received this e-mail in error and > that any use, dissemination, forwarding, printing, or copying of this > e-mail is strictly prohibited. If you have received this e-mail in > error, please immediately notify the sender via return e-mail or call > Brookhaven Memorial Hospital Medical Center at (631) 654-7282. > > > ------------------------------ > > Message: 5 > Date: Tue, 3 Sep 2013 15:38:14 +0000 > From: "Boyd, Debbie M" > Subject: [Histonet] RE: cost of slide > To: "Margiotta-Watz, Michele" , > "'histonet@lists.utsouthwestern.edu'" > > Message-ID: > <7EAFE982E328304DA6CE2B677BB76246794F6948@TN001WEXMBX12.US.chs.net> > Content-Type: text/plain; charset="us-ascii" > > Per recut slide $50.00. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Margiotta-Watz, Michele > Sent: Tuesday, September 03, 2013 11:36 AM > To: 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] cost of slide > > Hi All, > My pathologist wants to know how much other labs are charging for recutting an H&E slide to send out to a law firm for example. I know this has been discussed before on Histonet but I couldn't find any info. Your help is appreciated! > > Thanks, > Michele M. > Histology Supervisor > BMHMC > DISCLAIMER: > > This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -------------------------------------------------------------------------- > Disclaimer: This electronic message may contain information that is > Proprietary, Confidential, or legally privileged or protected. It > is intended only for the use of the individual(s) and entity named > in the message. If you are not an intended recipient of this > message, please notify the sender immediately and delete the > material from your computer. Do not deliver, distribute or copy > this message and do not disclose its contents or take any action in > reliance on the information it contains. > > > > ------------------------------ > > Message: 6 > Date: Tue, 3 Sep 2013 08:45:59 -0700 (PDT) > From: Rene J Buesa > Subject: Re: [Histonet] cost of slide > To: "Margiotta-Watz, Michele" , > "'histonet@lists.utsouthwestern.edu'" > > Message-ID: > <1378223159.34494.YahooMailNeo@web163106.mail.bf1.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Please visit http://www.histosearch.com/rene.html and you will find an article dealing with costs in histology. > You will have to work the numbers and increase material costs to current values and adjust for your salary base. > Ren? J. > > > ________________________________ > From: "Margiotta-Watz, Michele" > To: "'histonet@lists.utsouthwestern.edu'" > Sent: Tuesday, September 3, 2013 11:36 AM > Subject: [Histonet] cost of slide > > > Hi All, > My pathologist wants to know how much other labs are charging for recutting an H&E slide to send out to a law firm for example. I know this has been discussed before on Histonet but I couldn't find any info. Your help is appreciated! > > Thanks, > Michele M. > Histology Supervisor > BMHMC > DISCLAIMER: > > This e-mail and any files transmitted with it are confidential and are > intended solely for the use of the individual or entity to which they > are addressed. This communication may contain material protected by > the attorney-client privilege. If you are not the intended recipient > or the person responsible for delivering the e-mail to the intended > recipient, be advised that you have received this e-mail in error and > that any use, dissemination, forwarding, printing, or copying of this > e-mail is strictly prohibited. If you have received this e-mail in > error, please immediately notify the sender via return e-mail or call > Brookhaven Memorial Hospital Medical Center at (631) 654-7282. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > Message: 7 > Date: Tue, 3 Sep 2013 08:57:33 -0700 > From: William Chappell > Subject: [Histonet] Histologist Available for Southern California > Cc: histonet@lists.utsouthwestern.edu > Message-ID: <024740D2-A502-45CE-BE1E-133E38821C32@yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Greetings Histonet, > > I am writing this for a colleague of mine who is not on Histonet. > > I have a friend who is relocating from Seattle, Washington. He is an experienced HTL with extensive IHC experience (QIHC). He has over 3 years of Anatomic Pathology Management experience, but would jump at the chance for a bench position. > > He is looking for anything in San Diego, Riverside, Los Angeles, or San Bernardino county. If anybody, including temp recruiters, has anything, please contact me and I will forward it to my friend. > > Thanks, > > William Chappell, HTL(ASCP), QIHC > > > ------------------------------ > > Message: 8 > Date: Tue, 3 Sep 2013 12:56:18 -0400 > From: "Hannen, Valerie" > Subject: [Histonet] RE: Razor Blade Holder > To: 'Laurie Colbert' , "Histonet Post > (histonet@lists.utsouthwestern.edu)" > > Message-ID: <450B7A81EDA0C54E97C53D60F00776C3232B4BB647@isexstore03> > Content-Type: text/plain; charset="us-ascii" > > They are called Mini Scrapers. I have some that are from Mercedes Medical. There are 4/pk. > > > Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) > Histology Section Chief > Parrish Medical Center > 951 N. Washington Ave. > Titusville, Florida 32976 > Phone:(321) 268-6333 ext. 7506 > Fax: (321) 268-6149 > valerie.hannen@parrishmed.com > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert > Sent: Tuesday, September 03, 2013 10:16 AM > To: Histonet Post (histonet@lists.utsouthwestern.edu) > Subject: [Histonet] Razor Blade Holder > > Does anyone know where I can purchase the little plastic holder for razor blades for trimming your blocks and what the thing is called?? > > Laurie Colbert, HT (ASCP) > Histology Supervisor > PATH MD > 8158 Beverly Blvd. > Los Angeles, CA 90048 > (323) 648-3214 direct > (424) 245-7284 main lab > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > =================== > "This email is intended solely for the use of the individual to > whom it is addressed and may contain information that is > privileged, confidential or otherwise exempt from disclosure > under applicable law. If the reader of this email is not the > intended recipient or the employee or agent responsible for > delivering the message to the intended recipient, you are > hereby notified that any dissemination, distribution, or > copying of this communication is strictly prohibited. If you > have received this communication in error, please immediately > delete this message. Thank you" > =================== > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 118, Issue 2 > **************************************** From Valerie.Hannen <@t> parrishmed.com Tue Sep 3 12:09:35 2013 From: Valerie.Hannen <@t> parrishmed.com (Hannen, Valerie) Date: Tue Sep 3 12:09:40 2013 Subject: [Histonet] Eosin problem Message-ID: <450B7A81EDA0C54E97C53D60F00776C3232B4BB648@isexstore03> Hi All,' Hoping someone can give me some insight on if this happens/ happened to them. We change all of our reagents on the H&E stainer on Friday( which means the reagents sit idle over the weekend.) We rarely have to come in on the weekends due to having to embed/cut/stain tissues. However, on Monday or in this case today (Tues because of the Holiday), we have to stir our Eosin before putting our first rack through for staining because the Pathologists have been complained that the Eosin is too light if we don't. But I've had one of them tell me that a second and subsequent rack of slides does'nt have this problem. The Pathologist wants to know if by the tissue sitting in Formalin longer over the weekend on the processor vs. only sitting a few extra hours on the overnight run, is the extra time in Formalin causing this problem? I told him that I did'nt think so, but that I would ask. I also thought that maybe in addition to stirring the Eosin, maybe running a rack of "blank" slides through a staining program before puttng the patient tissue through might help. What do you all think?? Thanks so much!! Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.hannen@parrishmed.com =================== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" =================== From naje1972 <@t> yahoo.com Tue Sep 3 12:45:35 2013 From: naje1972 <@t> yahoo.com (cynthia haynes) Date: Tue Sep 3 12:45:46 2013 Subject: [Histonet] temp charts Message-ID: <1378230335.24364.YahooMailNeo@web122901.mail.ne1.yahoo.com> Hello All, ? Can someone tell where I can find temperature QC charts. My where erased from my email. From rjbuesa <@t> yahoo.com Tue Sep 3 16:47:19 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Sep 3 16:47:23 2013 Subject: [Histonet] Eosin problem In-Reply-To: <450B7A81EDA0C54E97C53D60F00776C3232B4BB648@isexstore03> References: <450B7A81EDA0C54E97C53D60F00776C3232B4BB648@isexstore03> Message-ID: <1378244839.2558.YahooMailNeo@web163101.mail.bf1.yahoo.com> Firstly: you should not schedule changing the eosin or any stain for that matter on a "time" (in this case) basis. The reagents ought to be changed on usage basis, i.e. establish a maximum slides to be stained before changing the reagents. Second: your pathologists are wrong because had the time in formalin any bearing on the problem, all the racks should come pale and not only the first. Third: it is very likely that during the weekend some sort of surface oxidation may occur and that is why stirring the eosin "solves" the problem. Four: Faced with this problem and if you insist on changing the reagents of a?time basis, change them on Mondays?even if somebody has to come on the weekend to section/stan Ren? J. ________________________________ From: "Hannen, Valerie" To: "Histonet Post (histonet@lists.utsouthwestern.edu)" Sent: Tuesday, September 3, 2013 1:09 PM Subject: [Histonet] Eosin problem Hi All,' Hoping someone can give me some insight on if this happens/ happened to them.? We change all of our reagents on the H&E stainer on Friday( which means the reagents sit idle over the weekend.)? We rarely have to come in on the weekends due to having to embed/cut/stain tissues.? However, on Monday or in this case today (Tues because of the Holiday), we have to stir our Eosin before putting our first rack through for staining because the Pathologists have been complained that the Eosin is too light if we don't.? But I've had one of them tell me that a second and subsequent rack of slides does'nt have this problem.? The Pathologist wants to know if by the tissue sitting in Formalin longer over the weekend on the processor vs. only sitting a few extra hours on the overnight run, is the extra time in Formalin causing this problem? I told him that I did'nt think so, but that I would ask.? I also thought that maybe in addition to stirring the Eosin, maybe running a rack of "blank" slides through a staining program before puttng the patient tissue through might help.? What do you all think?? Thanks so much!! Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.hannen@parrishmed.com ====================================="This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" =====================================_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amosbrooks <@t> gmail.com Tue Sep 3 18:36:35 2013 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Tue Sep 3 18:36:40 2013 Subject: [Histonet] Re: Histonet Digest, Vol 118, Issue 2 In-Reply-To: <5226159e.aa71b60a.49af.12a3SMTPIN_ADDED_MISSING@mx.google.com> References: <5226159e.aa71b60a.49af.12a3SMTPIN_ADDED_MISSING@mx.google.com> Message-ID: Hi Laurie, You can pick them up from Home Depot or Lowes. They are mini scrapers. Amos On Tue, Sep 3, 2013 at 1:00 PM, wrote: > Message: 2 > Date: Tue, 3 Sep 2013 14:16:13 +0000 > From: Laurie Colbert > Subject: [Histonet] Razor Blade Holder > To: "Histonet Post (histonet@lists.utsouthwestern.edu)" > > Message-ID: > > <12ECD7346266D74691EC2BFC75285E452F339F42@BFL323E10.pathmdlabs.local> > Content-Type: text/plain; charset="us-ascii" > > Does anyone know where I can purchase the little plastic holder for razor > blades for trimming your blocks and what the thing is called?? > > Laurie Colbert, HT (ASCP) > Histology Supervisor > From HornHV <@t> archildrens.org Wed Sep 4 07:42:13 2013 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Wed Sep 4 07:42:19 2013 Subject: [Histonet] cap Message-ID: <25A4DE08332B19499904459F00AAACB719C704FD42@EVS1.archildrens.org> CAP is in the house! Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From ihcs <@t> smithsys.net Tue Sep 3 20:35:54 2013 From: ihcs <@t> smithsys.net (ihcs-wojcieszyn) Date: Wed Sep 4 08:38:40 2013 Subject: [Histonet] This is not Facebook Message-ID: <56BF3E08-1502-11E3-ACE4-003065A5E3AC@smithsys.net> E-mails that tell all of what is happening, i.e. "CAP is in house" is a waste of time. Are we going to communicate when you wash your hands or have a cup of coffee. If Histonet is to be a source of information, ASK a question jw From billodonnell <@t> catholichealth.net Wed Sep 4 08:46:38 2013 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Wed Sep 4 08:47:06 2013 Subject: [Histonet] This is not Facebook In-Reply-To: <56BF3E08-1502-11E3-ACE4-003065A5E3AC@smithsys.net> References: <56BF3E08-1502-11E3-ACE4-003065A5E3AC@smithsys.net> Message-ID: Wow. :( OK, here's my question. CAP is in house here as well. How do they DO that? Bilocation, mitosis, time travel? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of ihcs-wojcieszyn Sent: Tuesday, September 03, 2013 8:36 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] This is not Facebook E-mails that tell all of what is happening, i.e. "CAP is in house" is a waste of time. Are we going to communicate when you wash your hands or have a cup of coffee. If Histonet is to be a source of information, ASK a question jw _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. From NMP <@t> stowers.org Wed Sep 4 08:48:12 2013 From: NMP <@t> stowers.org (Marsh, Nannette) Date: Wed Sep 4 08:48:15 2013 Subject: [Histonet] This is not Facebook In-Reply-To: <56BF3E08-1502-11E3-ACE4-003065A5E3AC@smithsys.net> References: <56BF3E08-1502-11E3-ACE4-003065A5E3AC@smithsys.net> Message-ID: <7A23A5A43C06284686B4868E88677530A52553@MBSRV02.sgc.loc> Wow, life is too short. We don't have to take things so seriously all of the time. I do hope your day gets better :-) Nanne Marsh HT ASCP -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of ihcs-wojcieszyn Sent: Tuesday, September 03, 2013 8:36 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] This is not Facebook E-mails that tell all of what is happening, i.e. "CAP is in house" is a waste of time. Are we going to communicate when you wash your hands or have a cup of coffee. If Histonet is to be a source of information, ASK a question jw _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From fred <@t> academic-urology.com Wed Sep 4 09:10:35 2013 From: fred <@t> academic-urology.com (Fred Fifield) Date: Wed Sep 4 09:10:40 2013 Subject: [Histonet] This is not Facebook In-Reply-To: <7A23A5A43C06284686B4868E88677530A52553@MBSRV02.sgc.loc> References: <56BF3E08-1502-11E3-ACE4-003065A5E3AC@smithsys.net> <7A23A5A43C06284686B4868E88677530A52553@MBSRV02.sgc.loc> Message-ID: I just washed my hands, but the coffee isn't ready yet. Have you ever noticed that watched pots never brew as quickly as you'd like? Fred Fifield B.S., HT(ASCP) Histology Supervisor Academic Urology & Urogynecology of Arizona 14044 W. Camelback Road Suite 218 Litchfield Park, AZ 85340 (623) 302-9301 fred@academic-urology.com On Wed, Sep 4, 2013 at 6:48 AM, Marsh, Nannette wrote: > Wow, life is too short. We don't have to take things so seriously all of > the time. I do hope your day gets better :-) > > Nanne Marsh HT ASCP > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of ihcs-wojcieszyn > Sent: Tuesday, September 03, 2013 8:36 PM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] This is not Facebook > > E-mails that tell all of what is happening, i.e. "CAP is in house" is a > waste of time. Are we going to communicate when you wash your hands or have > a cup of coffee. > If Histonet is to be a source of information, ASK a question > > > jw > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From mucram11 <@t> comcast.net Wed Sep 4 09:15:41 2013 From: mucram11 <@t> comcast.net (Pam Marcum) Date: Wed Sep 4 09:16:14 2013 Subject: [Histonet] EPIC Beaker Message-ID: <2077860955.846186.1378304141697.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> I know asked this a couple of months ago however; we are just getting ready to work with the EPIC people on the Histology/IHC/Slide & Block/Gross Room issues we need in AP.? I am looking for any advise or recommendations anyone who is running Epic Beaker now or is in the process as we are to implement it in the next 6 months or so.? I would prefer this is off line if anyone resonding is OK doing it that way.? If others want the info then please to Histonet will work too.? We are having issues getting our IT group and Epic to understand AP is not the same as CP. Thank You, Pam Marcum UAMS Histology Supervisor From jqb7 <@t> cdc.gov Wed Sep 4 09:18:02 2013 From: jqb7 <@t> cdc.gov (Sanders, Jeanine (CDC/OID/NCEZID)) Date: Wed Sep 4 09:18:57 2013 Subject: [Histonet] This is not Facebook In-Reply-To: References: <56BF3E08-1502-11E3-ACE4-003065A5E3AC@smithsys.net> <7A23A5A43C06284686B4868E88677530A52553@MBSRV02.sgc.loc> Message-ID: It doesn't take a rocket surgeon to figure that one out! :) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fred Fifield Sent: Wednesday, September 04, 2013 10:11 AM To: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] This is not Facebook I just washed my hands, but the coffee isn't ready yet. Have you ever noticed that watched pots never brew as quickly as you'd like? Fred Fifield B.S., HT(ASCP) Histology Supervisor Academic Urology & Urogynecology of Arizona 14044 W. Camelback Road Suite 218 Litchfield Park, AZ 85340 (623) 302-9301 fred@academic-urology.com On Wed, Sep 4, 2013 at 6:48 AM, Marsh, Nannette wrote: > Wow, life is too short. We don't have to take things so seriously all > of the time. I do hope your day gets better :-) > > Nanne Marsh HT ASCP > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > ihcs-wojcieszyn > Sent: Tuesday, September 03, 2013 8:36 PM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] This is not Facebook > > E-mails that tell all of what is happening, i.e. "CAP is in house" is > a waste of time. Are we going to communicate when you wash your hands > or have a cup of coffee. > If Histonet is to be a source of information, ASK a question > > > jw > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ASelf <@t> georgetownhospitalsystem.org Wed Sep 4 09:20:59 2013 From: ASelf <@t> georgetownhospitalsystem.org (Amy Self) Date: Wed Sep 4 09:21:05 2013 Subject: [Histonet] This is not Facebook In-Reply-To: References: <56BF3E08-1502-11E3-ACE4-003065A5E3AC@smithsys.net> <7A23A5A43C06284686B4868E88677530A52553@MBSRV02.sgc.loc> Message-ID: Wow !!!! There is medicine for stuff like that... Amy Self Histology Lab Senior Tech Lab Georgetown Memorial Hospital 606 Black River Road Georgetown, SC 29440 843-520-8711 ASelf@georgetownhospitalsystem.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sanders, Jeanine (CDC/OID/NCEZID) Sent: Wednesday, September 04, 2013 10:18 AM To: Fred Fifield; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] This is not Facebook It doesn't take a rocket surgeon to figure that one out! :) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fred Fifield Sent: Wednesday, September 04, 2013 10:11 AM To: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] This is not Facebook I just washed my hands, but the coffee isn't ready yet. Have you ever noticed that watched pots never brew as quickly as you'd like? Fred Fifield B.S., HT(ASCP) Histology Supervisor Academic Urology & Urogynecology of Arizona 14044 W. Camelback Road Suite 218 Litchfield Park, AZ 85340 (623) 302-9301 fred@academic-urology.com On Wed, Sep 4, 2013 at 6:48 AM, Marsh, Nannette wrote: > Wow, life is too short. We don't have to take things so seriously all > of the time. I do hope your day gets better :-) > > Nanne Marsh HT ASCP > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > ihcs-wojcieszyn > Sent: Tuesday, September 03, 2013 8:36 PM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] This is not Facebook > > E-mails that tell all of what is happening, i.e. "CAP is in house" is > a waste of time. Are we going to communicate when you wash your hands > or have a cup of coffee. > If Histonet is to be a source of information, ASK a question > > > jw > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. From mward <@t> wakehealth.edu Wed Sep 4 10:14:30 2013 From: mward <@t> wakehealth.edu (Martha Ward-Pathology) Date: Wed Sep 4 10:14:43 2013 Subject: [Histonet] EPIC Beaker In-Reply-To: <2077860955.846186.1378304141697.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> References: <2077860955.846186.1378304141697.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Message-ID: I would be interested in any information as well. We do not plan to implement for a year or more but one of our hospitals is working on Beaker now and finding that it does not adapt to AP as well as it does CP. ? Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard ?\? Winston-Salem, NC 27157 p 336.716.2109 ?\? f 336.716.5890 ? mward@wakehealth.edu ? ? ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pam Marcum Sent: Wednesday, September 04, 2013 10:16 AM To: Histonet Subject: [Histonet] EPIC Beaker I know asked this a couple of months ago however; we are just getting ready to work with the EPIC people on the Histology/IHC/Slide & Block/Gross Room issues we need in AP.? I am looking for any advise or recommendations anyone who is running Epic Beaker now or is in the process as we are to implement it in the next 6 months or so.? I would prefer this is off line if anyone resonding is OK doing it that way.? If others want the info then please to Histonet will work too.? We are having issues getting our IT group and Epic to understand AP is not the same as CP. Thank You, Pam Marcum UAMS Histology Supervisor _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TJohnson <@t> gnf.org Wed Sep 4 10:28:46 2013 From: TJohnson <@t> gnf.org (Teri Johnson) Date: Wed Sep 4 10:28:50 2013 Subject: [Histonet] Re: Cap is in the house Message-ID: <9F3CFEE76E51B64991C7485270890B40497BD026@EX5.lj.gnf.org> Good morning from warm, muggy San Diego! I understand why some folks have a bit of a short fuse with superfluous seeming emails from the Histonet. It's the same reason we are not allowed to send emails out to all associates at work. Most have an over-active email account anyway and it's just another one that takes their time to read. For those folks, I recommend subscribing to the Histonet digest. You get one, maybe two emails a day only, with the complete compilation of the day's questions, comments, witty retorts, etc. You can visit here: http://lists.utsouthwestern.edu/mailman/listinfo/histonet and change your mail delivery. Otherwise, I recommend a quick scan and liberal use of the delete button. Teri Johnson Manager, Histology GNF - San Diego, CA 858-332-4752 From amber.mckenzie <@t> gastrodocs.net Wed Sep 4 10:40:31 2013 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Wed Sep 4 10:40:39 2013 Subject: [Histonet] dish drying wall mount In-Reply-To: <9F3CFEE76E51B64991C7485270890B40497BD026@EX5.lj.gnf.org> References: <9F3CFEE76E51B64991C7485270890B40497BD026@EX5.lj.gnf.org> Message-ID: <5A33C952BB67F4468AF1F36D739212BCBD6E214E@JERRY.Gia.com> Does anyone know where I can find a wall mount that you put your clean dishes on to dry? We currently just lay a paper towel on the cabinet to let our dishes dry on, but I'd like to find a the wall mount that has areas where you can hang the items up to dry. Thanks! From Joyce.Weems <@t> emoryhealthcare.org Wed Sep 4 10:57:52 2013 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Wed Sep 4 10:57:59 2013 Subject: [Histonet] RE: dish drying wall mount In-Reply-To: <5A33C952BB67F4468AF1F36D739212BCBD6E214E@JERRY.Gia.com> References: <9F3CFEE76E51B64991C7485270890B40497BD026@EX5.lj.gnf.org> <5A33C952BB67F4468AF1F36D739212BCBD6E214E@JERRY.Gia.com> Message-ID: http://www.mercedesmedical.com/default.aspx?page=item+detail&itemcode=FIS+S47855 They don't have an image, but I think this is probably what you want. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Wednesday, September 04, 2013 11:41 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] dish drying wall mount Does anyone know where I can find a wall mount that you put your clean dishes on to dry? We currently just lay a paper towel on the cabinet to let our dishes dry on, but I'd like to find a the wall mount that has areas where you can hang the items up to dry. Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From Pat.Bell <@t> ucdenver.edu Wed Sep 4 11:25:15 2013 From: Pat.Bell <@t> ucdenver.edu (Bell, Pat) Date: Wed Sep 4 11:31:49 2013 Subject: [Histonet] RE: dish drying wall mount In-Reply-To: <5A33C952BB67F4468AF1F36D739212BCBD6E214E@JERRY.Gia.com> References: <9F3CFEE76E51B64991C7485270890B40497BD026@EX5.lj.gnf.org> <5A33C952BB67F4468AF1F36D739212BCBD6E214E@JERRY.Gia.com> Message-ID: <64DB27005E2FD3439E88502D7A5C9121010246103A26@CORTEZ.ucdenver.pvt> VWR has one. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Wednesday, September 04, 2013 9:41 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] dish drying wall mount Does anyone know where I can find a wall mount that you put your clean dishes on to dry? We currently just lay a paper towel on the cabinet to let our dishes dry on, but I'd like to find a the wall mount that has areas where you can hang the items up to dry. Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lowenjeff <@t> hotmail.com Wed Sep 4 11:37:14 2013 From: lowenjeff <@t> hotmail.com (jeff lowen) Date: Wed Sep 4 11:37:22 2013 Subject: [Histonet] RE: dish drying wall mount In-Reply-To: <64DB27005E2FD3439E88502D7A5C9121010246103A26@CORTEZ.ucdenver.pvt> References: <9F3CFEE76E51B64991C7485270890B40497BD026@EX5.lj.gnf.org>, <5A33C952BB67F4468AF1F36D739212BCBD6E214E@JERRY.Gia.com>, <64DB27005E2FD3439E88502D7A5C9121010246103A26@CORTEZ.ucdenver.pvt> Message-ID: I think everyone offers it. In a small lab, the type with pegs to hold each piece works well > From: Pat.Bell@ucdenver.edu > To: amber.mckenzie@gastrodocs.net; histonet@lists.utsouthwestern.edu > Date: Wed, 4 Sep 2013 10:25:15 -0600 > CC: > Subject: [Histonet] RE: dish drying wall mount > > VWR has one. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie > Sent: Wednesday, September 04, 2013 9:41 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] dish drying wall mount > > > Does anyone know where I can find a wall mount that you put your clean dishes on to dry? We currently just lay a paper towel on the cabinet to let our dishes dry on, but I'd like to find a the wall mount that has areas where you can hang the items up to dry. Thanks! > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hymclab.hymclab <@t> ministryhealth.org Wed Sep 4 11:54:38 2013 From: hymclab.hymclab <@t> ministryhealth.org (hymclab) Date: Wed Sep 4 11:54:48 2013 Subject: [Histonet] This is not Facebook In-Reply-To: <7A23A5A43C06284686B4868E88677530A52553@MBSRV02.sgc.loc> References: <56BF3E08-1502-11E3-ACE4-003065A5E3AC@smithsys.net> <7A23A5A43C06284686B4868E88677530A52553@MBSRV02.sgc.loc> Message-ID: I responded to jw privately but want to show my support for Hazel or anyone else that is facing a CAP inspection. I think Hazel and everyone that posts that CAP is in their institution is looking for moral support and I sent Hazel a message right away saying "Good luck". I hope Hazel or anyone else posts how their inspection goes and lets the rest of us know any issues we should be on the look out for!~!!!! We all know how trying it can be to be inspected!!!! Just hit delete if you don't want to be friedly and show your support!!! Dawn D. Schneider, HT(ASCP) Lead HT Howard Young Medical Center 240 Maple Ave. Woodruff, WI 54558 715-356-8174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marsh, Nannette Sent: Wednesday, September 04, 2013 8:48 AM To: ihcs-wojcieszyn; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] This is not Facebook Wow, life is too short. We don't have to take things so seriously all of the time. I do hope your day gets better :-) Nanne Marsh HT ASCP -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of ihcs-wojcieszyn Sent: Tuesday, September 03, 2013 8:36 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] This is not Facebook E-mails that tell all of what is happening, i.e. "CAP is in house" is a waste of time. Are we going to communicate when you wash your hands or have a cup of coffee. If Histonet is to be a source of information, ASK a question jw _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. From Vickroy.Jim <@t> mhsil.com Wed Sep 4 12:10:27 2013 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Wed Sep 4 12:10:34 2013 Subject: [Histonet] Tissue Processor Message-ID: Wondered if anyone had any thoughts or information on the comparison of two tissue processors that we are considering. The two instruments are the Leica ASP6025 and the Thermofisher Excelsior ES. We originally also considered another VIP. The Leica ASP6025 and the Thermofisher unit have a lot of similarities but there are some definite differences including price. I wish there was a Consumer Reports study of tissue processors because making the correct choice is getting harder and harder. Our tissue processors run every day and I definitely want an instrument to be able to take the wear and tear of every day usage. Obviously the VIP 6 has a family of processors that have lasted for years but I'm not sure that they have kept up with the new technology and bells and whistles that make usage easier and more cost-effective. If you have any experience with one of these processors please let me know, good or bad. I have been in histology for over 35 years and this is the first time I have felt "like I'm buying a car". There are so many rumors out there such as: 1. You have to use proprietary reagents with this processor because the easy fill only works with their bottles. (recall) 2. One instrument will not last as long as the other because of materials in the design. 3. Service will be a problem with one company and not the other, because of who is trained to service the instrument. 4. Etc, Etc, Etc. I would be forever grateful for any additional information you could give me. Thanks James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From histotalk <@t> yahoo.com Wed Sep 4 12:28:59 2013 From: histotalk <@t> yahoo.com (David Kemler) Date: Wed Sep 4 12:29:04 2013 Subject: [Histonet] HistoTA:LK / 2013 Symposium-Convention Message-ID: <1378315739.91735.YahooMailNeo@web121502.mail.ne1.yahoo.com> Hello Everyone - ? HistoTALK will be setting up shop again this year to do some interviews. If you have something to shout about on internet radio, here's your opportunity. The "mobile" HistoTALK (all digital this time around) studio will be available on the following days and times: ? Saturday, September 21 - 9 am to 3 pm Sunday,?? September 22 - 9 am to 3 pm Monday,? September 23 - 9 am to 12 noon Tuesday,? September 24 - 12 noon to 3 pm ? Interviews can be anywhere from 30 minutes, up to an hour! The time is YOURS! ? Let me know if you are interested and the day and time that fits your schedule. ? Yours, Dave From sdysart <@t> mirnarx.com Wed Sep 4 12:32:33 2013 From: sdysart <@t> mirnarx.com (Sarah Dysart) Date: Wed Sep 4 12:32:42 2013 Subject: [Histonet] This is not Facebook In-Reply-To: References: <56BF3E08-1502-11E3-ACE4-003065A5E3AC@smithsys.net> <7A23A5A43C06284686B4868E88677530A52553@MBSRV02.sgc.loc> Message-ID: <975554c7ed774475b592ea8063009b6c@BY2PR07MB106.namprd07.prod.outlook.com> Well said =) Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of hymclab Sent: Wednesday, September 04, 2013 11:55 AM To: 'Marsh, Nannette'; ihcs-wojcieszyn; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] This is not Facebook I responded to jw privately but want to show my support for Hazel or anyone else that is facing a CAP inspection. I think Hazel and everyone that posts that CAP is in their institution is looking for moral support and I sent Hazel a message right away saying "Good luck". I hope Hazel or anyone else posts how their inspection goes and lets the rest of us know any issues we should be on the look out for!~!!!! We all know how trying it can be to be inspected!!!! Just hit delete if you don't want to be friedly and show your support!!! Dawn D. Schneider, HT(ASCP) Lead HT Howard Young Medical Center 240 Maple Ave. Woodruff, WI 54558 715-356-8174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marsh, Nannette Sent: Wednesday, September 04, 2013 8:48 AM To: ihcs-wojcieszyn; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] This is not Facebook Wow, life is too short. We don't have to take things so seriously all of the time. I do hope your day gets better :-) Nanne Marsh HT ASCP -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of ihcs-wojcieszyn Sent: Tuesday, September 03, 2013 8:36 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] This is not Facebook E-mails that tell all of what is happening, i.e. "CAP is in house" is a waste of time. Are we going to communicate when you wash your hands or have a cup of coffee. If Histonet is to be a source of information, ASK a question jw _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpjones <@t> srhs-pa.org Wed Sep 4 12:33:05 2013 From: lpjones <@t> srhs-pa.org (Jones, Laura) Date: Wed Sep 4 12:32:48 2013 Subject: [Histonet] Processors and IHC Stainers Message-ID: <4AE8039AEA096143B965CBC6D09216680249C1366C@EXCH2007.srhs-pa.org> We are looking/hoping for some opinions on some equipment that we are considering for our lab. In the tissue processor line up we have: Leica's ASP6025; the VIP 6; Thermo's STP 420ES and Milestone's Logos. The IHC Stainer contenders are: Ventana's Ultra; Leica's Bond III or an updated version of what we currently use - the Labvision Autostainer. It's especially important to us to hear the opinions of techs who work with these instruments, as the selections here are being made primarily by those who do not. We are a community hospital with about 5800 cases/year. We average 25-30 IHC slides/day. Thanks in advance! ________________________________ Sharon Regional Health System is the area's largest hospital and provider of health care services. Visit us online at http://www.sharonregional.com for a complete listing of our services, primary care physicians and specialists, and satellite locations. Confidentiality Note: This message is intended for use only by the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. Thank you. From rjbuesa <@t> yahoo.com Wed Sep 4 13:29:42 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Sep 4 13:29:46 2013 Subject: [Histonet] Processors and IHC Stainers In-Reply-To: <4AE8039AEA096143B965CBC6D09216680249C1366C@EXCH2007.srhs-pa.org> References: <4AE8039AEA096143B965CBC6D09216680249C1366C@EXCH2007.srhs-pa.org> Message-ID: <1378319382.97325.YahooMailNeo@web163106.mail.bf1.yahoo.com> VIP6 for a more "classical" processing or the Milestone's Logos if you want faster turnaround times. Leica's Bond III Ren? J. ________________________________ From: "Jones, Laura" To: "Histonet@lists.utsouthwestern.edu" Sent: Wednesday, September 4, 2013 1:33 PM Subject: [Histonet] Processors and IHC Stainers We are looking/hoping for some opinions on some equipment that we are considering for our lab. In the tissue processor line up we have:? Leica's ASP6025; the VIP 6; Thermo's STP 420ES and Milestone's Logos. The IHC Stainer contenders are:? Ventana's Ultra; Leica's Bond III or an updated version of what we currently use - the Labvision Autostainer. It's especially important to us to hear the opinions of techs who work with these instruments, as the selections here are being made primarily by those who do not.? We are a community hospital with about 5800 cases/year.? We average 25-30 IHC slides/day.? Thanks in advance! ________________________________ Sharon Regional Health System is the area's largest hospital and provider of health care services. Visit us online at http://www.sharonregional.com/for a complete listing of our services, primary care physicians and specialists, and satellite locations. Confidentiality Note: This message is intended for use only by the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LSebree <@t> uwhealth.org Wed Sep 4 13:43:17 2013 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Wed Sep 4 13:43:20 2013 Subject: [Histonet] RE: Processors and IHC Stainers In-Reply-To: <4AE8039AEA096143B965CBC6D09216680249C1366C@EXCH2007.srhs-pa.org> References: <4AE8039AEA096143B965CBC6D09216680249C1366C@EXCH2007.srhs-pa.org> Message-ID: <77DD817201982748BC67D7960F2F76AF06517C@UWHC-MBX12.uwhis.hosp.wisc.edu> We like our 4 Ventana Ultras for IHC, ISH and CISH. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jones, Laura Sent: Wednesday, September 04, 2013 12:33 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Processors and IHC Stainers We are looking/hoping for some opinions on some equipment that we are considering for our lab. In the tissue processor line up we have: Leica's ASP6025; the VIP 6; Thermo's STP 420ES and Milestone's Logos. The IHC Stainer contenders are: Ventana's Ultra; Leica's Bond III or an updated version of what we currently use - the Labvision Autostainer. It's especially important to us to hear the opinions of techs who work with these instruments, as the selections here are being made primarily by those who do not. We are a community hospital with about 5800 cases/year. We average 25-30 IHC slides/day. Thanks in advance! ________________________________ Sharon Regional Health System is the area's largest hospital and provider of health care services. Visit us online at http://www.sharonregional.com for a complete listing of our services, primary care physicians and specialists, and satellite locations. Confidentiality Note: This message is intended for use only by the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From subash.govender <@t> uct.ac.za Wed Sep 4 16:41:16 2013 From: subash.govender <@t> uct.ac.za (Subash Govender) Date: Wed Sep 4 16:41:29 2013 Subject: [Histonet] RE: holes in nuclei of FFPE tissue after IHC staining Message-ID: <32CD6307BAB24F4DAD1C6E6B3E8AA22343E97487@SRVWINEXC004.wf.uct.ac.za> Dear All I am having a problem with my immunohstochemistry staining. The nuclei of the cells have holes in the middle. I have a feeling that it could be my pressure cooking procedure. I pressure cook the slides for 1min 30 secs and leave them to stand for 20 mins thereafter. This is strange because i did not always have this problem. The cells seem to be overcooked. This occurs with both EDTA pH 8,9 and citric acid pH6 solutions. Has anybody had this problem or can anyone help please. Thank you very much. Subash Govender Medical Technologist Anatomical Pathology Research Lab Medical School University of Cape Town South Africa Tel: +27 21 4066599 email: subash.govender@uct.ac.za ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of histonet-request@lists.utsouthwestern.edu [histonet-request@lists.utsouthwestern.edu] Sent: 04 September 2013 07:01 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 118, Issue 4 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: This is not Facebook (hymclab) ---------------------------------------------------------------------- Message: 1 Date: Wed, 4 Sep 2013 11:54:38 -0500 From: hymclab Subject: RE: [Histonet] This is not Facebook To: "'Marsh, Nannette'" , ihcs-wojcieszyn , "Histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" I responded to jw privately but want to show my support for Hazel or anyone else that is facing a CAP inspection. I think Hazel and everyone that posts that CAP is in their institution is looking for moral support and I sent Hazel a message right away saying "Good luck". I hope Hazel or anyone else posts how their inspection goes and lets the rest of us know any issues we should be on the look out for!~!!!! We all know how trying it can be to be inspected!!!! Just hit delete if you don't want to be friedly and show your support!!! Dawn D. Schneider, HT(ASCP) Lead HT Howard Young Medical Center 240 Maple Ave. Woodruff, WI 54558 715-356-8174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marsh, Nannette Sent: Wednesday, September 04, 2013 8:48 AM To: ihcs-wojcieszyn; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] This is not Facebook Wow, life is too short. We don't have to take things so seriously all of the time. I do hope your day gets better :-) Nanne Marsh HT ASCP -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of ihcs-wojcieszyn Sent: Tuesday, September 03, 2013 8:36 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] This is not Facebook E-mails that tell all of what is happening, i.e. "CAP is in house" is a waste of time. Are we going to communicate when you wash your hands or have a cup of coffee. If Histonet is to be a source of information, ASK a question jw _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 118, Issue 4 **************************************** ________________________________ UNIVERSITY OF CAPE TOWN This e-mail is subject to the UCT ICT policies and e-mail disclaimer published on our website at http://www.uct.ac.za/about/policies/emaildisclaimer/ or obtainable from +27 21 650 9111. This e-mail is intended only for the person(s) to whom it is addressed. If the e-mail has reached you in error, please notify the author. If you are not the intended recipient of the e-mail you may not use, disclose, copy, redirect or print the content. If this e-mail is not related to the business of UCT it is sent by the sender in the sender's individual capacity. From patpxs <@t> gmail.com Wed Sep 4 16:45:07 2013 From: patpxs <@t> gmail.com (Paula Sicurello) Date: Wed Sep 4 16:45:18 2013 Subject: [Histonet] EPIC Beaker In-Reply-To: <2077860955.846186.1378304141697.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> References: <2077860955.846186.1378304141697.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Message-ID: See if you can get your Beaker people to talk to the Beaker people who are working on the Duke University Health System-ClinLabs AP build. We are taking it one step at a time and the AP module is being built separately from the CP build. Beaker is taking over 18 months to build, we have tentative go-live for July 2014. Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 HIPAA Privacy Notification: This message and any accompanying documents are covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, and contain information intended for the specific individual (s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying or the taking of any action based on the contents of this information is strictly prohibited . If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. On Wed, Sep 4, 2013 at 10:15 AM, Pam Marcum wrote: > > > > I know asked this a couple of months ago however; we are just getting > ready to work with the EPIC people on the Histology/IHC/Slide & Block/Gross > Room issues we need in AP. I am looking for any advise or recommendations > anyone who is running Epic Beaker now or is in the process as we are to > implement it in the next 6 months or so. I would prefer this is off line > if anyone resonding is OK doing it that way. If others want the info then > please to Histonet will work too. > > > > We are having issues getting our IT group and Epic to understand AP is not > the same as CP. > > > > Thank You, > > Pam Marcum > > UAMS Histology Supervisor > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From bbrinegarhtl <@t> gmail.com Wed Sep 4 16:53:45 2013 From: bbrinegarhtl <@t> gmail.com (Beth Brinegar) Date: Wed Sep 4 16:53:55 2013 Subject: [Histonet] EPIC Beaker In-Reply-To: References: <2077860955.846186.1378304141697.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Message-ID: Good afternoon! We just went live with Epic Beaker June 2013 and it's been one fat mess. I have plenty of information for anyone interested. It seems there were lots of things that weren't considered for AP with Epic beaker. We have faced a lot of problems with go-live. My first piece of advice is to keep all you pathologists VERY involved with the build structure and to consult with Optime frequently. We've had the most difficulty with Optime and Beaker "talking" and specimens getting lost in Epic. Or, we've had AP specimens get lost in core lab, and everything has to be reordered to get things moving. Please feel free to contact me with any questions. The build is a big beast, and we didn't have much success with our go-live because there were many things we didn't really know to consider. Beth Brinegar HTL(ASCP) Anatomic Pathology Supervisor 319-432-2239 (cell) Beth Brinegar HTL(ASCP) Anatomic Pathology Supervisor Mercy Medical Center Cedar Rapids, IA 52403 On Wed, Sep 4, 2013 at 4:45 PM, Paula Sicurello wrote: > See if you can get your Beaker people to talk to the Beaker people who are > working on the Duke University Health System-ClinLabs AP build. We are > taking it one step at a time and the AP module is being built separately > from the CP build. > > Beaker is taking over 18 months to build, we have tentative go-live for > July 2014. > > Paula > > -- > Paula Sicurello, HTL (ASCP) > Supervisor, Clinical Electron Microscopy Laboratory > Duke University Health System > Rm.#251M, Duke South, Green Zone > Durham, North Carolina 27710 > P: 919.684.2091 > HIPAA Privacy Notification: This message and any accompanying documents are > covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, > and contain information intended for the specific individual (s) only. This > information is confidential. If you are not the intended recipient or an > agent responsible for delivering it to the intended recipient, you are > hereby notified that you have received this document in error and that any > review, dissemination, copying or the taking of any action based on the > contents of this information is strictly prohibited . If you have received > this communication in error, please notify us immediately by e-mail, and > delete the original message. > > > On Wed, Sep 4, 2013 at 10:15 AM, Pam Marcum wrote: > > > > > > > > > I know asked this a couple of months ago however; we are just getting > > ready to work with the EPIC people on the Histology/IHC/Slide & > Block/Gross > > Room issues we need in AP. I am looking for any advise or > recommendations > > anyone who is running Epic Beaker now or is in the process as we are to > > implement it in the next 6 months or so. I would prefer this is off line > > if anyone resonding is OK doing it that way. If others want the info > then > > please to Histonet will work too. > > > > > > > > We are having issues getting our IT group and Epic to understand AP is > not > > the same as CP. > > > > > > > > Thank You, > > > > Pam Marcum > > > > UAMS Histology Supervisor > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From daniela.bodemer <@t> mcri.edu.au Wed Sep 4 18:14:52 2013 From: daniela.bodemer <@t> mcri.edu.au (Daniela Bodemer) Date: Wed Sep 4 18:15:04 2013 Subject: [Histonet] unsubscribe Message-ID: <39B17DC4575F1C42B358BE0C40FD3DFB053EF8@MITSMXMBX01V.mcri.edu.au> I would like to unsubscribe to this list Regards Daniela Bodemer Research Assistant Surgical Research, Infection & Immunity Murdoch Childrens Research Institute The Royal Children's Hospital Flemington Road Parkville Victoria 3052 Australia E daniela.bodemer@mcri.edu.au www.mcri.edu.au Celebrating 25 Years of Life Saving Discoveries This e-mail and any attachments to it (the "Communication") are, unless otherwise stated, confidential, may contain copyright material and is for the use only of the intended recipient. If you receive the Communication in error, please notify the sender immediately by return e-mail, delete the Communication and the return e-mail, and do not read, copy, retransmit or otherwise deal with it. Any views expressed in the Communication are those of the individual sender only, unless expressly stated to be those of Murdoch Childrens Research Institute (MCRI) ABN 21 006 566 972 or any of its related entities. MCRI does not accept liability in connection with the integrity of or errors in the Communication, computer virus, data corruption, interference or delay arising from or in respect of the Communication. P Please consider the environment before printing this email ______________________________________________________________________ This email has been scanned by the Symantec Email Security.cloud service. For more information please visit http://www.symanteccloud.com ______________________________________________________________________ From jaylundgren <@t> gmail.com Wed Sep 4 23:19:27 2013 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Wed Sep 4 23:19:35 2013 Subject: [Histonet] unsubscribe In-Reply-To: <39B17DC4575F1C42B358BE0C40FD3DFB053EF8@MITSMXMBX01V.mcri.edu.au> References: <39B17DC4575F1C42B358BE0C40FD3DFB053EF8@MITSMXMBX01V.mcri.edu.au> Message-ID: lol On Wed, Sep 4, 2013 at 6:14 PM, Daniela Bodemer wrote: > I would like to unsubscribe to this list > > Regards > > Daniela Bodemer > Research Assistant > Surgical Research, Infection & Immunity > > Murdoch Childrens Research Institute > The Royal Children's Hospital > Flemington Road Parkville Victoria 3052 Australia > E daniela.bodemer@mcri.edu.au > www.mcri.edu.au > > Celebrating 25 Years of Life Saving Discoveries > > This e-mail and any attachments to it (the "Communication") are, unless > otherwise stated, confidential, may contain copyright material and is for > the use only of the intended recipient. If you receive the Communication in > error, please notify the sender immediately by return e-mail, delete the > Communication and the return e-mail, and do not read, copy, retransmit or > otherwise deal with it. Any views expressed in the Communication are those > of the individual sender only, unless expressly stated to be those of > Murdoch Childrens Research Institute (MCRI) ABN 21 006 566 972 or any of > its related entities. MCRI does not accept liability in connection with the > integrity of or errors in the Communication, computer virus, data > corruption, interference or delay arising from or in respect of the > Communication. > > P Please consider the environment before printing this email > > > ______________________________________________________________________ > This email has been scanned by the Symantec Email Security.cloud service. > For more information please visit http://www.symanteccloud.com > ______________________________________________________________________ > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From HornHV <@t> archildrens.org Thu Sep 5 09:07:39 2013 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Thu Sep 5 09:07:46 2013 Subject: [Histonet] please delete if you are not interested. CAP questions from yesterday. Message-ID: <25A4DE08332B19499904459F00AAACB719C704FD4A@EVS1.archildrens.org> The inspector was very thorough. And most of the questions he had were report related. He asked for a report showing correlation of cytology results with histology results, a report showing the outside consultations are listed in the surg path report, evidence logs for cryostat decontamination, reports showing the cancer protocols are using synoptic format, records for the QC of antibodies, antibody validation logs, instrument service records, etc. While most of those are not technically histology related, I had to find the records showing the policies/procedures are being followed. Overall we did well. We were cited for proficiency testing a phase I. Years ago we did the IHC surveys but stopped doing them because we lacked the antibodies most of the surveys asked for as we are children's hospital and most of them are geared toward adult diagnosis. I just learned about the QIP program and I am excited for us to participate. I am sorry I didn't know this survey existed. We will participate again in the IHC surveys. I appreciate the support I receive from the histonet and the information I receive here helps me have a better lab and be a better manager. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sarah Dysart Sent: Wednesday, September 04, 2013 12:33 PM To: hymclab; 'Marsh, Nannette'; ihcs-wojcieszyn; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] This is not Facebook Well said =) Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of hymclab Sent: Wednesday, September 04, 2013 11:55 AM To: 'Marsh, Nannette'; ihcs-wojcieszyn; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] This is not Facebook I responded to jw privately but want to show my support for Hazel or anyone else that is facing a CAP inspection. I think Hazel and everyone that posts that CAP is in their institution is looking for moral support and I sent Hazel a message right away saying "Good luck". I hope Hazel or anyone else posts how their inspection goes and lets the rest of us know any issues we should be on the look out for!~!!!! We all know how trying it can be to be inspected!!!! Just hit delete if you don't want to be friedly and show your support!!! Dawn D. Schneider, HT(ASCP) Lead HT Howard Young Medical Center 240 Maple Ave. Woodruff, WI 54558 715-356-8174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marsh, Nannette Sent: Wednesday, September 04, 2013 8:48 AM To: ihcs-wojcieszyn; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] This is not Facebook Wow, life is too short. We don't have to take things so seriously all of the time. I do hope your day gets better :-) Nanne Marsh HT ASCP -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of ihcs-wojcieszyn Sent: Tuesday, September 03, 2013 8:36 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] This is not Facebook E-mails that tell all of what is happening, i.e. "CAP is in house" is a waste of time. Are we going to communicate when you wash your hands or have a cup of coffee. If Histonet is to be a source of information, ASK a question jw _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From robinsoc <@t> mercyhealth.com Thu Sep 5 09:33:24 2013 From: robinsoc <@t> mercyhealth.com (Cynthia Robinson) Date: Thu Sep 5 09:33:34 2013 Subject: [Histonet] please delete if you are not interested. CAP questions from yesterday. In-Reply-To: <25A4DE08332B19499904459F00AAACB719C704FD4A@EVS1.archildrens.org> References: <25A4DE08332B19499904459F00AAACB719C704FD4A@EVS1.archildrens.org> Message-ID: <52284FE4020000AF0000FAF6@nodcdmg2.no.trinity-health.org> Hazel, Thanks for sharing the information about your CAP experience. With very few outside learning opportunities at our facility I always thought CAP inspections were a way of sharing information on tests, processes, etc. When I have been inspecting I feel rushed to get thru the packet and those learning moments don't happen because of the time constraints. Congratulations to you and your team! Cindi Robinson HT(ASCP) Mercy Medical Center Dunes Medical Laboratories 350 W Anchor Dr Dakota Dunes SD 57049 phone-712-279-2768 robinsoc@mercyhealth.com >>> "Horn, Hazel V" 9/5/2013 9:07 AM >>> The inspector was very thorough. And most of the questions he had were report related. He asked for a report showing correlation of cytology results with histology results, a report showing the outside consultations are listed in the surg path report, evidence logs for cryostat decontamination, reports showing the cancer protocols are using synoptic format, records for the QC of antibodies, antibody validation logs, instrument service records, etc. While most of those are not technically histology related, I had to find the records showing the policies/procedures are being followed. Overall we did well. We were cited for proficiency testing a phase I. Years ago we did the IHC surveys but stopped doing them because we lacked the antibodies most of the surveys asked for as we are children's hospital and most of them are geared toward adult diagnosis. I just learned about the QIP program and I am excited for us to participate. I am sorry I didn't know this survey existed. We will participate again in the IHC surveys. I appreciate the support I receive from the histonet and the information I receive here helps me have a better lab and be a better manager. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sarah Dysart Sent: Wednesday, September 04, 2013 12:33 PM To: hymclab; 'Marsh, Nannette'; ihcs-wojcieszyn; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] This is not Facebook Well said =) Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of hymclab Sent: Wednesday, September 04, 2013 11:55 AM To: 'Marsh, Nannette'; ihcs-wojcieszyn; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] This is not Facebook I responded to jw privately but want to show my support for Hazel or anyone else that is facing a CAP inspection. I think Hazel and everyone that posts that CAP is in their institution is looking for moral support and I sent Hazel a message right away saying "Good luck". I hope Hazel or anyone else posts how their inspection goes and lets the rest of us know any issues we should be on the look out for!~!!!! We all know how trying it can be to be inspected!!!! Just hit delete if you don't want to be friedly and show your support!!! Dawn D. Schneider, HT(ASCP) Lead HT Howard Young Medical Center 240 Maple Ave. Woodruff, WI 54558 715-356-8174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marsh, Nannette Sent: Wednesday, September 04, 2013 8:48 AM To: ihcs-wojcieszyn; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] This is not Facebook Wow, life is too short. We don't have to take things so seriously all of the time. I do hope your day gets better :-) Nanne Marsh HT ASCP -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of ihcs-wojcieszyn Sent: Tuesday, September 03, 2013 8:36 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] This is not Facebook E-mails that tell all of what is happening, i.e. "CAP is in house" is a waste of time. Are we going to communicate when you wash your hands or have a cup of coffee. If Histonet is to be a source of information, ASK a question jw _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PAMarcum <@t> uams.edu Thu Sep 5 09:46:06 2013 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Thu Sep 5 09:46:11 2013 Subject: [Histonet] please delete if you are not interested. CAP questions from yesterday. In-Reply-To: <25A4DE08332B19499904459F00AAACB719C704FD4A@EVS1.archildrens.org> References: <25A4DE08332B19499904459F00AAACB719C704FD4A@EVS1.archildrens.org> Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA32D31576B1@Mail2Node2.ad.uams.edu> Congratulations Hazel and ACH!!! It is good to know what they are asking now as the inspection checklists have changed and that affects all of us. Pam Marcum -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Thursday, September 05, 2013 9:08 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] please delete if you are not interested. CAP questions from yesterday. The inspector was very thorough. And most of the questions he had were report related. He asked for a report showing correlation of cytology results with histology results, a report showing the outside consultations are listed in the surg path report, evidence logs for cryostat decontamination, reports showing the cancer protocols are using synoptic format, records for the QC of antibodies, antibody validation logs, instrument service records, etc. While most of those are not technically histology related, I had to find the records showing the policies/procedures are being followed. Overall we did well. We were cited for proficiency testing a phase I. Years ago we did the IHC surveys but stopped doing them because we lacked the antibodies most of the surveys asked for as we are children's hospital and most of them are geared toward adult diagnosis. I just learned about the QIP program and I am excited for us to participate. I am sorry I didn't know this survey existed. We will participate again in the IHC surveys. I appreciate the support I receive from the histonet and the information I receive here helps me have a better lab and be a better manager. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sarah Dysart Sent: Wednesday, September 04, 2013 12:33 PM To: hymclab; 'Marsh, Nannette'; ihcs-wojcieszyn; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] This is not Facebook Well said =) Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of hymclab Sent: Wednesday, September 04, 2013 11:55 AM To: 'Marsh, Nannette'; ihcs-wojcieszyn; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] This is not Facebook I responded to jw privately but want to show my support for Hazel or anyone else that is facing a CAP inspection. I think Hazel and everyone that posts that CAP is in their institution is looking for moral support and I sent Hazel a message right away saying "Good luck". I hope Hazel or anyone else posts how their inspection goes and lets the rest of us know any issues we should be on the look out for!~!!!! We all know how trying it can be to be inspected!!!! Just hit delete if you don't want to be friedly and show your support!!! Dawn D. Schneider, HT(ASCP) Lead HT Howard Young Medical Center 240 Maple Ave. Woodruff, WI 54558 715-356-8174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marsh, Nannette Sent: Wednesday, September 04, 2013 8:48 AM To: ihcs-wojcieszyn; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] This is not Facebook Wow, life is too short. We don't have to take things so seriously all of the time. I do hope your day gets better :-) Nanne Marsh HT ASCP -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of ihcs-wojcieszyn Sent: Tuesday, September 03, 2013 8:36 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] This is not Facebook E-mails that tell all of what is happening, i.e. "CAP is in house" is a waste of time. Are we going to communicate when you wash your hands or have a cup of coffee. If Histonet is to be a source of information, ASK a question jw _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From tjrichmond <@t> hughes.net Thu Sep 5 09:48:48 2013 From: tjrichmond <@t> hughes.net (Tonia Richmond) Date: Thu Sep 5 09:48:52 2013 Subject: [Histonet] unsubscribe In-Reply-To: References: <39B17DC4575F1C42B358BE0C40FD3DFB053EF8@MITSMXMBX01V.mcri.edu.au> Message-ID: Me too please Best Regards, Tonia Richmond Ph: (870) 489-2939 On Sep 4, 2013, at 11:19 PM, Jay Lundgren wrote: > lol > > > On Wed, Sep 4, 2013 at 6:14 PM, Daniela Bodemer > wrote: > >> I would like to unsubscribe to this list >> >> Regards >> >> Daniela Bodemer >> Research Assistant >> Surgical Research, Infection & Immunity >> >> Murdoch Childrens Research Institute >> The Royal Children's Hospital >> Flemington Road Parkville Victoria 3052 Australia >> E daniela.bodemer@mcri.edu.au >> www.mcri.edu.au >> >> Celebrating 25 Years of Life Saving Discoveries >> >> This e-mail and any attachments to it (the "Communication") are, unless >> otherwise stated, confidential, may contain copyright material and is for >> the use only of the intended recipient. If you receive the Communication in >> error, please notify the sender immediately by return e-mail, delete the >> Communication and the return e-mail, and do not read, copy, retransmit or >> otherwise deal with it. Any views expressed in the Communication are those >> of the individual sender only, unless expressly stated to be those of >> Murdoch Childrens Research Institute (MCRI) ABN 21 006 566 972 or any of >> its related entities. MCRI does not accept liability in connection with the >> integrity of or errors in the Communication, computer virus, data >> corruption, interference or delay arising from or in respect of the >> Communication. >> >> P Please consider the environment before printing this email >> >> >> ______________________________________________________________________ >> This email has been scanned by the Symantec Email Security.cloud service. >> For more information please visit http://www.symanteccloud.com >> ______________________________________________________________________ >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akbitting <@t> geisinger.edu Thu Sep 5 11:04:56 2013 From: akbitting <@t> geisinger.edu (Bitting, Angela K.) Date: Thu Sep 5 11:05:06 2013 Subject: [Histonet] This is not Facebook In-Reply-To: References: <56BF3E08-1502-11E3-ACE4-003065A5E3AC@smithsys.net> <7A23A5A43C06284686B4868E88677530A52553@MBSRV02.sgc.loc> Message-ID: <77F52EFAB8B1694B885E277C48FCD0F64C4ABEA7@GHSEXMBX4W8K1V.geisinger.edu> Yeah, wow, I just read through this thread.... I guess haters are gonna hate. Nobody's got time for that. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of hymclab Sent: Wednesday, September 04, 2013 12:55 PM To: 'Marsh, Nannette'; ihcs-wojcieszyn; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] This is not Facebook I responded to jw privately but want to show my support for Hazel or anyone else that is facing a CAP inspection. I think Hazel and everyone that posts that CAP is in their institution is looking for moral support and I sent Hazel a message right away saying "Good luck". I hope Hazel or anyone else posts how their inspection goes and lets the rest of us know any issues we should be on the look out for!~!!!! We all know how trying it can be to be inspected!!!! Just hit delete if you don't want to be friedly and show your support!!! Dawn D. Schneider, HT(ASCP) Lead HT Howard Young Medical Center 240 Maple Ave. Woodruff, WI 54558 715-356-8174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marsh, Nannette Sent: Wednesday, September 04, 2013 8:48 AM To: ihcs-wojcieszyn; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] This is not Facebook Wow, life is too short. We don't have to take things so seriously all of the time. I do hope your day gets better :-) Nanne Marsh HT ASCP -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of ihcs-wojcieszyn Sent: Tuesday, September 03, 2013 8:36 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] This is not Facebook E-mails that tell all of what is happening, i.e. "CAP is in house" is a waste of time. Are we going to communicate when you wash your hands or have a cup of coffee. If Histonet is to be a source of information, ASK a question jw _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. From lowenjeff <@t> hotmail.com Thu Sep 5 11:16:44 2013 From: lowenjeff <@t> hotmail.com (jeff lowen) Date: Thu Sep 5 11:16:50 2013 Subject: [Histonet] This is not Facebook In-Reply-To: <77F52EFAB8B1694B885E277C48FCD0F64C4ABEA7@GHSEXMBX4W8K1V.geisinger.edu> References: <56BF3E08-1502-11E3-ACE4-003065A5E3AC@smithsys.net>, <7A23A5A43C06284686B4868E88677530A52553@MBSRV02.sgc.loc>, , <77F52EFAB8B1694B885E277C48FCD0F64C4ABEA7@GHSEXMBX4W8K1V.geisinger.edu> Message-ID: That's right, we gotta get back to our microtomes.....lol > From: akbitting@geisinger.edu > To: hymclab.hymclab@ministryhealth.org; NMP@stowers.org; ihcs@smithsys.net; Histonet@lists.utsouthwestern.edu > Date: Thu, 5 Sep 2013 16:04:56 +0000 > Subject: RE: [Histonet] This is not Facebook > CC: > > Yeah, wow, I just read through this thread.... I guess haters are gonna hate. Nobody's got time for that. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of hymclab > Sent: Wednesday, September 04, 2013 12:55 PM > To: 'Marsh, Nannette'; ihcs-wojcieszyn; Histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] This is not Facebook > > I responded to jw privately but want to show my support for Hazel or anyone else that is facing a CAP inspection. I think Hazel and everyone that posts that CAP is in their institution is looking for moral support and I sent Hazel a message right away saying "Good luck". I hope Hazel or anyone else posts how their inspection goes and lets the rest of us know any issues we should be on the look out for!~!!!! > > We all know how trying it can be to be inspected!!!! Just hit delete if you don't want to be friedly and show your support!!! > > > Dawn D. Schneider, HT(ASCP) > Lead HT > Howard Young Medical Center > 240 Maple Ave. > Woodruff, WI 54558 > 715-356-8174 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marsh, Nannette > Sent: Wednesday, September 04, 2013 8:48 AM > To: ihcs-wojcieszyn; Histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] This is not Facebook > > Wow, life is too short. We don't have to take things so seriously all of the time. I do hope your day gets better :-) > > Nanne Marsh HT ASCP > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of ihcs-wojcieszyn > Sent: Tuesday, September 03, 2013 8:36 PM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] This is not Facebook > > E-mails that tell all of what is happening, i.e. "CAP is in house" is a waste of time. Are we going to communicate when you wash your hands or have a cup of coffee. > If Histonet is to be a source of information, ASK a question > > > jw > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. > > Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Sep 5 11:17:42 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Sep 5 11:19:32 2013 Subject: [Histonet] This is not Facebook In-Reply-To: <77F52EFAB8B1694B885E277C48FCD0F64C4ABEA7@GHSEXMBX4W8K1V.geisinger.edu> References: <56BF3E08-1502-11E3-ACE4-003065A5E3AC@smithsys.net> <7A23A5A43C06284686B4868E88677530A52553@MBSRV02.sgc.loc> <77F52EFAB8B1694B885E277C48FCD0F64C4ABEA7@GHSEXMBX4W8K1V.geisinger.edu> Message-ID: <1378397862.79441.YahooMailNeo@web163102.mail.bf1.yahoo.com> >From my SOP: "In the event that you do not like something nor?are interested in something posted in HistoNet, just DELETE" Ren? J. ________________________________ From: "Bitting, Angela K." To: hymclab ; "'Marsh, Nannette'" ; ihcs-wojcieszyn ; "Histonet@lists.utsouthwestern.edu" Sent: Thursday, September 5, 2013 12:04 PM Subject: RE: [Histonet] This is not Facebook Yeah, wow, I just read through this thread.... I guess haters are gonna hate. Nobody's got time for that. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of hymclab Sent: Wednesday, September 04, 2013 12:55 PM To: 'Marsh, Nannette'; ihcs-wojcieszyn; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] This is not Facebook I responded to jw privately but want to show my support for Hazel or anyone else that is facing a CAP inspection.? I think Hazel and everyone that posts that CAP is in their institution is looking for moral support and I sent Hazel a message right away saying "Good luck".? I hope Hazel or anyone else posts how their inspection goes and lets the rest of us know any issues we should be on the look out for!~!!!! We all know how trying it can be to be inspected!!!!? Just hit delete if you don't want to be friedly and show your support!!! Dawn D. Schneider, HT(ASCP) Lead HT Howard Young Medical Center 240 Maple Ave. Woodruff, WI 54558 715-356-8174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marsh, Nannette Sent: Wednesday, September 04, 2013 8:48 AM To: ihcs-wojcieszyn; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] This is not Facebook Wow, life is too short.? We don't have to take things so seriously all of the time.? I do hope your day gets better :-) Nanne Marsh HT ASCP -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of ihcs-wojcieszyn Sent: Tuesday, September 03, 2013 8:36 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] This is not Facebook E-mails that tell all of what is happening, i.e. "CAP is in house" is a waste of time. Are we going to communicate when you wash your hands or have a cup of coffee. If Histonet is to be a source of information, ASK a question jw _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sccrshlly <@t> yahoo.com Thu Sep 5 11:12:13 2013 From: sccrshlly <@t> yahoo.com (Shelly Coker) Date: Thu Sep 5 11:22:58 2013 Subject: [Histonet] RE: Message-ID: <1378397533.80005.YahooMailNeo@web140202.mail.bf1.yahoo.com> http://icff.ir/24th/en/tmp/twitter.php?ymrdj977dpa.htm sccrshlly Shelly Coker ********** I'd forget my balls if they didn't come in a bag. From thisisann <@t> aol.com Thu Sep 5 11:45:11 2013 From: thisisann <@t> aol.com (Ann Specian) Date: Thu Sep 5 11:45:15 2013 Subject: [Histonet] Cell Block Preparation Message-ID: <8D0786E6C1AA835-ECC-D72C@Webmail-d108.sysops.aol.com> I am getting complaints in regard to "insufficient" cell blocks. We currently spin, pour off the supernatant, retrieve the sediment and process in lens paper. Does anyone have a more current technique which renders better cellularity? Also, do you know which renders a better cell block: a fresh specimen, a specimen fixed in Cytolyt or a specimen fixed in 10% NBF? Thanks, Ann From avistarop <@t> ffyb.uba.ar Thu Sep 5 12:45:14 2013 From: avistarop <@t> ffyb.uba.ar (avistarop@ffyb.uba.ar) Date: Thu Sep 5 12:45:33 2013 Subject: [Histonet] unsubscribe In-Reply-To: References: <39B17DC4575F1C42B358BE0C40FD3DFB053EF8@MITSMXMBX01V.mcri.edu.au> Message-ID: <3efea44a939ae577a4fa95aebd638eea.squirrel@huemul.ffyb.uba.ar> Me too please Best Regards, Aldana Vistarop > Ph: (870) 489-2939 > > On Sep 4, 2013, at 11:19 PM, Jay Lundgren wrote: > >> lol >> >> >> On Wed, Sep 4, 2013 at 6:14 PM, Daniela Bodemer >> >> wrote: >> >>> I would like to unsubscribe to this list >>> >>> Regards >>> >>> Daniela Bodemer >>> Research Assistant >>> Surgical Research, Infection & Immunity >>> >>> Murdoch Childrens Research Institute >>> The Royal Children's Hospital >>> Flemington Road Parkville Victoria 3052 Australia >>> E daniela.bodemer@mcri.edu.au >>> www.mcri.edu.au >>> >>> Celebrating 25 Years of Life Saving Discoveries >>> >>> This e-mail and any attachments to it (the "Communication") are, unless >>> otherwise stated, confidential, may contain copyright material and is >>> for >>> the use only of the intended recipient. If you receive the >>> Communication in >>> error, please notify the sender immediately by return e-mail, delete >>> the >>> Communication and the return e-mail, and do not read, copy, retransmit >>> or >>> otherwise deal with it. Any views expressed in the Communication are >>> those >>> of the individual sender only, unless expressly stated to be those of >>> Murdoch Childrens Research Institute (MCRI) ABN 21 006 566 972 or any >>> of >>> its related entities. MCRI does not accept liability in connection with >>> the >>> integrity of or errors in the Communication, computer virus, data >>> corruption, interference or delay arising from or in respect of the >>> Communication. >>> >>> P Please consider the environment before printing this email >>> >>> >>> ______________________________________________________________________ >>> This email has been scanned by the Symantec Email Security.cloud >>> service. >>> For more information please visit http://www.symanteccloud.com >>> ______________________________________________________________________ >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Joyce.Weems <@t> emoryhealthcare.org Thu Sep 5 12:54:20 2013 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Thu Sep 5 12:55:53 2013 Subject: [Histonet] unsubscribe In-Reply-To: <3efea44a939ae577a4fa95aebd638eea.squirrel@huemul.ffyb.uba.ar> References: <39B17DC4575F1C42B358BE0C40FD3DFB053EF8@MITSMXMBX01V.mcri.edu.au> <3efea44a939ae577a4fa95aebd638eea.squirrel@huemul.ffyb.uba.ar> Message-ID: You must go to the web site at the bottom of this email and unsubscribe. Here it is again... http://lists.utsouthwestern.edu/mailman/listinfo/histonet Cheers! Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of avistarop@ffyb.uba.ar Sent: Thursday, September 05, 2013 1:45 PM To: Tonia Richmond Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] unsubscribe Me too please Best Regards, Aldana Vistarop > Ph: (870) 489-2939 > > On Sep 4, 2013, at 11:19 PM, Jay Lundgren wrote: > >> lol >> >> >> On Wed, Sep 4, 2013 at 6:14 PM, Daniela Bodemer >> >> wrote: >> >>> I would like to unsubscribe to this list >>> >>> Regards >>> >>> Daniela Bodemer >>> Research Assistant >>> Surgical Research, Infection & Immunity >>> >>> Murdoch Childrens Research Institute The Royal Children's Hospital >>> Flemington Road Parkville Victoria 3052 Australia E >>> daniela.bodemer@mcri.edu.au >>> www.mcri.edu.au >>> >>> Celebrating 25 Years of Life Saving Discoveries >>> >>> This e-mail and any attachments to it (the "Communication") are, >>> unless otherwise stated, confidential, may contain copyright >>> material and is for the use only of the intended recipient. If you >>> receive the Communication in error, please notify the sender >>> immediately by return e-mail, delete the Communication and the >>> return e-mail, and do not read, copy, retransmit or otherwise deal >>> with it. Any views expressed in the Communication are those of the >>> individual sender only, unless expressly stated to be those of >>> Murdoch Childrens Research Institute (MCRI) ABN 21 006 566 972 or >>> any of its related entities. MCRI does not accept liability in >>> connection with the integrity of or errors in the Communication, >>> computer virus, data corruption, interference or delay arising from >>> or in respect of the Communication. >>> >>> P Please consider the environment before printing this email >>> >>> >>> ____________________________________________________________________ >>> __ This email has been scanned by the Symantec Email Security.cloud >>> service. >>> For more information please visit http://www.symanteccloud.com >>> ____________________________________________________________________ >>> __ _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From Jessica.Vacca <@t> HCAhealthcare.com Thu Sep 5 13:28:38 2013 From: Jessica.Vacca <@t> HCAhealthcare.com (Jessica.Vacca@HCAhealthcare.com) Date: Thu Sep 5 13:28:44 2013 Subject: [Histonet] This is not Facebook In-Reply-To: <1378397862.79441.YahooMailNeo@web163102.mail.bf1.yahoo.com> References: <56BF3E08-1502-11E3-ACE4-003065A5E3AC@smithsys.net> <7A23A5A43C06284686B4868E88677530A52553@MBSRV02.sgc.loc> <77F52EFAB8B1694B885E277C48FCD0F64C4ABEA7@GHSEXMBX4W8K1V.geisinger.edu> <1378397862.79441.YahooMailNeo@web163102.mail.bf1.yahoo.com> Message-ID: <938D716CD445614ABBB817517557B6F407F0F0D429@NADCWPMSGCMS09.hca.corpad.net> Does anyone know where the "LIKE" button is? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, September 05, 2013 12:18 PM To: Bitting, Angela K.; hymclab; 'Marsh, Nannette'; ihcs-wojcieszyn; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] This is not Facebook >From my SOP: "In the event that you do not like something nor?are interested in something posted in HistoNet, just DELETE" Ren? J. ________________________________ From: "Bitting, Angela K." To: hymclab ; "'Marsh, Nannette'" ; ihcs-wojcieszyn ; "Histonet@lists.utsouthwestern.edu" Sent: Thursday, September 5, 2013 12:04 PM Subject: RE: [Histonet] This is not Facebook Yeah, wow, I just read through this thread.... I guess haters are gonna hate. Nobody's got time for that. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of hymclab Sent: Wednesday, September 04, 2013 12:55 PM To: 'Marsh, Nannette'; ihcs-wojcieszyn; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] This is not Facebook I responded to jw privately but want to show my support for Hazel or anyone else that is facing a CAP inspection.? I think Hazel and everyone that posts that CAP is in their institution is looking for moral support and I sent Hazel a message right away saying "Good luck".? I hope Hazel or anyone else posts how their inspection goes and lets the rest of us know any issues we should be on the look out for!~!!!! We all know how trying it can be to be inspected!!!!? Just hit delete if you don't want to be friedly and show your support!!! Dawn D. Schneider, HT(ASCP) Lead HT Howard Young Medical Center 240 Maple Ave. Woodruff, WI 54558 715-356-8174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marsh, Nannette Sent: Wednesday, September 04, 2013 8:48 AM To: ihcs-wojcieszyn; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] This is not Facebook Wow, life is too short.? We don't have to take things so seriously all of the time.? I do hope your day gets better :-) Nanne Marsh HT ASCP -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of ihcs-wojcieszyn Sent: Tuesday, September 03, 2013 8:36 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] This is not Facebook E-mails that tell all of what is happening, i.e. "CAP is in house" is a waste of time. Are we going to communicate when you wash your hands or have a cup of coffee. If Histonet is to be a source of information, ASK a question jw _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bakevictoria <@t> gmail.com Thu Sep 5 13:36:38 2013 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Thu Sep 5 13:36:43 2013 Subject: [Histonet] This is not Facebook In-Reply-To: <938D716CD445614ABBB817517557B6F407F0F0D429@NADCWPMSGCMS09.hca.corpad.net> References: <56BF3E08-1502-11E3-ACE4-003065A5E3AC@smithsys.net> <7A23A5A43C06284686B4868E88677530A52553@MBSRV02.sgc.loc> <77F52EFAB8B1694B885E277C48FCD0F64C4ABEA7@GHSEXMBX4W8K1V.geisinger.edu> <1378397862.79441.YahooMailNeo@web163102.mail.bf1.yahoo.com> <938D716CD445614ABBB817517557B6F407F0F0D429@NADCWPMSGCMS09.hca.corpad.net> Message-ID: ;-). It's always worked for me as the "like" button(s). Just cheerie looking. On Sep 5, 2013 2:28 PM, wrote: > Does anyone know where the "LIKE" button is? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa > Sent: Thursday, September 05, 2013 12:18 PM > To: Bitting, Angela K.; hymclab; 'Marsh, Nannette'; ihcs-wojcieszyn; > Histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] This is not Facebook > > >From my SOP: > "In the event that you do not like something nor are interested in > something posted in HistoNet, just DELETE" > Ren? J. > > > ________________________________ > From: "Bitting, Angela K." > To: hymclab ; "'Marsh, Nannette'" < > NMP@stowers.org>; ihcs-wojcieszyn ; " > Histonet@lists.utsouthwestern.edu" > Sent: Thursday, September 5, 2013 12:04 PM > Subject: RE: [Histonet] This is not Facebook > > > Yeah, wow, I just read through this thread.... I guess haters are gonna > hate. Nobody's got time for that. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of hymclab > Sent: Wednesday, September 04, 2013 12:55 PM > To: 'Marsh, Nannette'; ihcs-wojcieszyn; Histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] This is not Facebook > > I responded to jw privately but want to show my support for Hazel or > anyone else that is facing a CAP inspection. I think Hazel and everyone > that posts that CAP is in their institution is looking for moral support > and I sent Hazel a message right away saying "Good luck". I hope Hazel or > anyone else posts how their inspection goes and lets the rest of us know > any issues we should be on the look out for!~!!!! > > We all know how trying it can be to be inspected!!!! Just hit delete if > you don't want to be friedly and show your support!!! > > > Dawn D. Schneider, HT(ASCP) > Lead HT > Howard Young Medical Center > 240 Maple Ave. > Woodruff, WI 54558 > 715-356-8174 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marsh, Nannette > Sent: Wednesday, September 04, 2013 8:48 AM > To: ihcs-wojcieszyn; Histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] This is not Facebook > > Wow, life is too short. We don't have to take things so seriously all of > the time. I do hope your day gets better :-) > > Nanne Marsh HT ASCP > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of ihcs-wojcieszyn > Sent: Tuesday, September 03, 2013 8:36 PM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] This is not Facebook > > E-mails that tell all of what is happening, i.e. "CAP is in house" is a > waste of time. Are we going to communicate when you wash your hands or have > a cup of coffee. > If Histonet is to be a source of information, ASK a question > > > jw > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may > contain confidential and privileged information for the use of the > designated recipient(s) named above. If you are not the intended recipient, > you are hereby notified that you have received this communication in error > and that any review, disclosure, dissemination, distribution or copying of > it or its contents is prohibited. If you have received this communication > in error, please notify the sender at the electronic mail address noted > above and destroy all copies of this communication and any attachments. > Thank you for your cooperation. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > IMPORTANT WARNING: The information in this message (and the documents > attached to it, if any) is confidential and may be legally privileged. It > is intended solely for the addressee. Access to this message by anyone else > is unauthorized. If you are not the intended recipient, any disclosure, > copying, distribution or any action taken, or omitted to be taken, in > reliance on it is prohibited and may be unlawful. If you have received this > message in error, please delete all electronic copies of this message (and > the documents attached to it, if any), destroy any hard copies you may have > created and notify me immediately by replying to this email. Thank you. > > Geisinger Health System utilizes an encryption process to safeguard > Protected Health Information and other confidential data contained in > external e-mail messages. If email is encrypted, the recipient will receive > an e-mail instructing them to sign on to the Geisinger Health System Secure > E-mail Message Center to retrieve the encrypted e-mail. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From lindamargraf <@t> gmail.com Thu Sep 5 14:02:41 2013 From: lindamargraf <@t> gmail.com (Linda Margraf) Date: Thu Sep 5 14:03:01 2013 Subject: [Histonet] Fwd: position available References: <2F06BE5D1D5D4F4991755CC53C0A4CBD48016675@WLMMBS02.nemours.org> Message-ID: <8ACA87E8-FF37-4CBA-86EF-67DF9F4B845D@gmail.com> Here's a message I posting for Carol....... Sent from my iPhone Begin forwarded message: > From: "Barone, Carol" > Date: August 27, 2013, 12:40:39 PM CDT > To: "'histonet-owner@lists.utsouthwestern.edu'" > Subject: position available > > Assistant Director Histotechnology Core Service, Nemours ? A.I. duPont Hospital for Children. > Required: > ? HT/HTL with BA or BS > ? 3-5 yrs experience in muscle enzyme histochemistry (Clinical) > ? 1-3 yrs experience in Immuno-histochemistry (IHC and IF) manual, optimization/validation cross species > ? CISH, FISH troubleshooting > ? Insitu- PCR and RT-PCR > ? Some management experience/ CLIA/ SOP?s > ? > Desired: ( some experience) > ? LMD/LCM > ? TMA > ? Cytology technical service > > From nmhisto <@t> comcast.net Thu Sep 5 20:56:37 2013 From: nmhisto <@t> comcast.net (nmhisto@comcast.net) Date: Thu Sep 5 20:57:01 2013 Subject: [Histonet] Unsubscribe, Chapter 195 In-Reply-To: <1755295187.1029338.1378431833151.JavaMail.root@sz0075a.emeryville.ca.mail.comcast.net> Message-ID: <1200426006.1029812.1378432597832.JavaMail.root@sz0075a.emeryville.ca.mail.comcast.net> It is a concern that members of our? technically-oriented career field ?have a difficult time understanding the method for unsubscribing to Histonet.? There is an ?almost- daily posting to "unsubscribe", despite the fact that this subject has been addressed literally hundreds of times.? When one "joins" Histonet, instructions are provided, should be printed out for reference and used?if the subscriber decides to leave the group.? We are required to be knowledgeable on all manner of technical routines requiring detailed instructions and? Histonet is no less clear in the methods for joining and "un-joining".? Use them, please.? Fire away - I'm retired and I can take the flak!? I do miss my microtome, though... From ree3 <@t> leicester.ac.uk Fri Sep 6 03:55:30 2013 From: ree3 <@t> leicester.ac.uk (Edwards, Richard E.) Date: Fri Sep 6 03:56:06 2013 Subject: [Histonet] costing UK only Message-ID: <7722595275A4DD4FA225B92CDBF174A101B0D6D98004@EXC-MBX3.cfs.le.ac.uk> Hi All, I need to cost immunostaining etc., for a grant application, formalin fixed, paraffin processing, haematoxylin and eosin, 5 antibodies, 50+ biopsies, using standard ABC kit, or perhaps the Envision package, any help or figures, or sources of figures and help gratefully received, thanks. Richard Edwards From TMcNemar <@t> lmhealth.org Fri Sep 6 04:45:41 2013 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Fri Sep 6 04:45:25 2013 Subject: [Histonet] Cell Block Preparation In-Reply-To: <8D0786E6C1AA835-ECC-D72C@Webmail-d108.sysops.aol.com> References: <8D0786E6C1AA835-ECC-D72C@Webmail-d108.sysops.aol.com> Message-ID: This is how we do it now. In the old days, we used agar and to my mind, it is still the best way when you have scant material. - Spin in a conical tube and pour off - Melt an agar slant (we get TSA slant from micro) - Pour the agar into the conical tube and spin for 5 minutes - The agar will re-solidify and whatever sediment there is will be concentrated in the very tip of the cone - The agar will slide out of the centrifuge tube - Slice off the very tip and wrap in lens paper - Place the wrapped tip in a cassette and process as usual - Embed the specimen tip down and you are good to go... I still use this method today when I feel it necessary. Works great. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ann Specian Sent: Thursday, September 05, 2013 12:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cell Block Preparation I am getting complaints in regard to "insufficient" cell blocks. We currently spin, pour off the supernatant, retrieve the sediment and process in lens paper. Does anyone have a more current technique which renders better cellularity? Also, do you know which renders a better cell block: a fresh specimen, a specimen fixed in Cytolyt or a specimen fixed in 10% NBF? Thanks, Ann _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From trathborne <@t> somerset-healthcare.com Fri Sep 6 06:18:27 2013 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Fri Sep 6 06:18:58 2013 Subject: [Histonet] Cell Block Preparation In-Reply-To: References: <8D0786E6C1AA835-ECC-D72C@Webmail-d108.sysops.aol.com> Message-ID: <3AD061FE740D464FAC7BF6B5CFB75707A8A51D80@smcmail02.somerset-healthcare.com> I wonder if this method could be used with the product Histogel. Has anyone tried it? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Friday, September 06, 2013 5:46 AM To: 'Ann Specian'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Cell Block Preparation This is how we do it now. In the old days, we used agar and to my mind, it is still the best way when you have scant material. - Spin in a conical tube and pour off - Melt an agar slant (we get TSA slant from micro) - Pour the agar into the conical tube and spin for 5 minutes - The agar will re-solidify and whatever sediment there is will be concentrated in the very tip of the cone - The agar will slide out of the centrifuge tube - Slice off the very tip and wrap in lens paper - Place the wrapped tip in a cassette and process as usual - Embed the specimen tip down and you are good to go... I still use this method today when I feel it necessary. Works great. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ann Specian Sent: Thursday, September 05, 2013 12:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cell Block Preparation I am getting complaints in regard to "insufficient" cell blocks. We currently spin, pour off the supernatant, retrieve the sediment and process in lens paper. Does anyone have a more current technique which renders better cellularity? Also, do you know which renders a better cell block: a fresh specimen, a specimen fixed in Cytolyt or a specimen fixed in 10% NBF? Thanks, Ann _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From philip_manfre <@t> merck.com Fri Sep 6 06:30:53 2013 From: philip_manfre <@t> merck.com (Manfre, Philip) Date: Fri Sep 6 06:30:58 2013 Subject: [Histonet] Unsubscribe, Chapter 195 In-Reply-To: <1200426006.1029812.1378432597832.JavaMail.root@sz0075a.emeryville.ca.mail.comcast.net> References: <1755295187.1029338.1378431833151.JavaMail.root@sz0075a.emeryville.ca.mail.comcast.net> <1200426006.1029812.1378432597832.JavaMail.root@sz0075a.emeryville.ca.mail.comcast.net> Message-ID: <558A4571351D0C42BD923F403F4198C4B135BC24F9@USCTMXP51014.merck.com> Trained professionals should know by now that if you want to unsubscribe, you must type in all caps - UNSUBSCRIBE Philip Manfre, B.A., HT (ASCP) Associate Principal Scientist Merck Research Laboratories WP45-251 PO Box 4 West Point, PA 19486 215-652-9750 215-993-0383 (fax) philip_manfre@merck.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of nmhisto@comcast.net Sent: Thursday, September 05, 2013 9:57 PM To: HISTONET Subject: [Histonet] Unsubscribe, Chapter 195 It is a concern that members of our? technically-oriented career field ?have a difficult time understanding the method for unsubscribing to Histonet.? There is an ?almost- daily posting to "unsubscribe", despite the fact that this subject has been addressed literally hundreds of times.? When one "joins" Histonet, instructions are provided, should be printed out for reference and used?if the subscriber decides to leave the group.? We are required to be knowledgeable on all manner of technical routines requiring detailed instructions and? Histonet is no less clear in the methods for joining and "un-joining".? Use them, please.? Fire away - I'm retired and I can take the flak!? I do miss my microtome, though... _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From dhewitt <@t> hvhs.org Fri Sep 6 08:05:48 2013 From: dhewitt <@t> hvhs.org (Daniel Hewitt) Date: Fri Sep 6 08:05:56 2013 Subject: [Histonet] Cell Block Preparation In-Reply-To: <3AD061FE740D464FAC7BF6B5CFB75707A8A51D80@smcmail02.somerset-healthcare.com> Message-ID: <7DDB5AB36CBC574D8D680806E7BBE58B016945B3@MX-HVB-02.hvhs.org> We recently switched most of our cell blocks from agar to Histogel, works great. We use small disposable embedding mold, put the specimen in the bottom and add the histogel about half way up the mold, let it harden, pop it out and put it in the cass. Also cuts much better than agar. Daniel Hewitt Histology Supervisor, HVS 412-749-7371 This email, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, or an agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and delete and destroy all copies of the original message, including attachments. Please note that any views or opinions presented in this e-mail are solely those of the author and do not necessarily represent those of Heritage Valley Health System. The integrity and security of this message cannot be guaranteed on the internet. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Friday, September 06, 2013 7:18 AM To: 'Tom McNemar'; 'Ann Specian'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Cell Block Preparation I wonder if this method could be used with the product Histogel. Has anyone tried it? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Friday, September 06, 2013 5:46 AM To: 'Ann Specian'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Cell Block Preparation This is how we do it now. In the old days, we used agar and to my mind, it is still the best way when you have scant material. - Spin in a conical tube and pour off - Melt an agar slant (we get TSA slant from micro) - Pour the agar into the conical tube and spin for 5 minutes - The agar will re-solidify and whatever sediment there is will be concentrated in the very tip of the cone - The agar will slide out of the centrifuge tube - Slice off the very tip and wrap in lens paper - Place the wrapped tip in a cassette and process as usual - Embed the specimen tip down and you are good to go... I still use this method today when I feel it necessary. Works great. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ann Specian Sent: Thursday, September 05, 2013 12:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cell Block Preparation I am getting complaints in regard to "insufficient" cell blocks. We currently spin, pour off the supernatant, retrieve the sediment and process in lens paper. Does anyone have a more current technique which renders better cellularity? Also, do you know which renders a better cell block: a fresh specimen, a specimen fixed in Cytolyt or a specimen fixed in 10% NBF? Thanks, Ann _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From twebster <@t> CRH.org Fri Sep 6 09:11:18 2013 From: twebster <@t> CRH.org (Webster, Thomas S.) Date: Fri Sep 6 09:11:24 2013 Subject: [Histonet] Cell Block Preparation Message-ID: <7207186ED68FB542803CAF1CE6E82FF808AC7A@exmb1.crh.org> This is a pretty good method for scant specimens. I have even used it for CSFs that have malignancy with success. http://www.jove.com/video/1316/cell-block-preparation-from-cytology-specimen-with-predominance CONFIDENTIALITY NOTICE: This e-mail message, including all attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. You may NOT use, disclose, copy or disseminate this information. If you are not the intended recipient, please contact the sender by reply e-mail immediately. Please destroy all copies of the original message and all attachments. Your cooperation is greatly appreciated. Columbus Regional Hospital 2400 East 17th Street Columbus, Indiana 47201 From mjdessoye <@t> commonwealthhealth.net Fri Sep 6 11:39:01 2013 From: mjdessoye <@t> commonwealthhealth.net (Dessoye, Michael J) Date: Fri Sep 6 11:39:13 2013 Subject: [Histonet] Cell Block Preparation In-Reply-To: <8D0786E6C1AA835-ECC-D72C@Webmail-d108.sysops.aol.com> Message-ID: We have had mixed results in the past with cell blocks, but currently we are filtering the fluid through a biopsy bag and processing that. We seem to be retaining more specimen with that method (we have used sponges and lens paper in the past). Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdessoye@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 -----Original Message----- From: Ann Specian [mailto:thisisann@aol.com] Sent: Thursday, September 05, 2013 12:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cell Block Preparation I am getting complaints in regard to "insufficient" cell blocks. We currently spin, pour off the supernatant, retrieve the sediment and process in lens paper. Does anyone have a more current technique which renders better cellularity? Also, do you know which renders a better cell block: a fresh specimen, a specimen fixed in Cytolyt or a specimen fixed in 10% NBF? Thanks, Ann _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Commonwealth Health. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. From Timothy.Morken <@t> ucsfmedctr.org Fri Sep 6 11:48:55 2013 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Fri Sep 6 11:49:06 2013 Subject: using celloidin RE: [Histonet] Cell Block Preparation Message-ID: <761E2B5697F795489C8710BCC72141FF0987BA@ex07.net.ucsf.edu> Our cytology department uses a technique that involves coating a centrifuge tube with Celloidin then spinning the sample in that tube. The cellodin is then scored and the "bag" of celloidin containing the cell button is taken out and wrapped in paper to be processed in a cassette. It is a tricky and a bit time consuming, but no material is lost in processing. We do up to 10 samples a day that way. Reference: J Clin Pathol. 1982 May; 35(5): 574-576. A celloidin bag for the histological preparation of cytologic material. G Bussolati Tim Morken Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dessoye, Michael J Sent: Friday, September 06, 2013 9:39 AM To: Ann Specian; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Cell Block Preparation We have had mixed results in the past with cell blocks, but currently we are filtering the fluid through a biopsy bag and processing that. We seem to be retaining more specimen with that method (we have used sponges and lens paper in the past). Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdessoye@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 -----Original Message----- From: Ann Specian [mailto:thisisann@aol.com] Sent: Thursday, September 05, 2013 12:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cell Block Preparation I am getting complaints in regard to "insufficient" cell blocks. We currently spin, pour off the supernatant, retrieve the sediment and process in lens paper. Does anyone have a more current technique which renders better cellularity? Also, do you know which renders a better cell block: a fresh specimen, a specimen fixed in Cytolyt or a specimen fixed in 10% NBF? Thanks, Ann _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Commonwealth Health. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tony.auge <@t> gmail.com Fri Sep 6 14:28:27 2013 From: tony.auge <@t> gmail.com (Tony Auge) Date: Fri Sep 6 14:28:35 2013 Subject: [Histonet] Cell Block Preparation In-Reply-To: References: <8D0786E6C1AA835-ECC-D72C@Webmail-d108.sysops.aol.com> Message-ID: We spin the sample down, pour off supernatant. Resuspend in 5 drops thromboplastin and 4 drops human serum we receive from our phlebotomy lab. Let firm for 5 minutes and then fix in NBF. The agar suggestion probably works similarly if you can't get serum. On Sep 6, 2013 9:39 AM, "Dessoye, Michael J" < mjdessoye@commonwealthhealth.net> wrote: > We have had mixed results in the past with cell blocks, but currently we > are filtering the fluid through a biopsy bag and processing that. We > seem to be retaining more specimen with that method (we have used > sponges and lens paper in the past). > > Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General > Hospital | An Affiliate of Commonwealth Health | > mjdessoye@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, > PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 > > > -----Original Message----- > From: Ann Specian [mailto:thisisann@aol.com] > Sent: Thursday, September 05, 2013 12:45 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Cell Block Preparation > > > I am getting complaints in regard to "insufficient" cell blocks. We > currently spin, pour off the supernatant, retrieve the sediment and > process in lens paper. > > Does anyone have a more current technique which renders better > cellularity? > > Also, do you know which renders a better cell block: a fresh specimen, > a specimen fixed in Cytolyt or a specimen fixed in 10% NBF? > > Thanks, > Ann > > _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ > > This email and any files transmitted with it are confidential and > intended solely for the use of the individual or entity to whom > they are addressed. > If you have received this email in error please notify the > originator of the message. This footer also confirms that this > email message has been scanned for the presence of computer viruses. > > Any views expressed in this message are those of the individual > sender, except where the sender specifies and with authority, > states them to be the views of Commonwealth Health. > > Scanning of this message and addition of this footer is performed > by Websense Email Security software in conjunction with > virus detection software. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Bruce_Palmatier <@t> vwr.com Fri Sep 6 15:01:40 2013 From: Bruce_Palmatier <@t> vwr.com (Bruce_Palmatier@vwr.com) Date: Fri Sep 6 15:01:50 2013 Subject: [Histonet] AUTO: Bruce Palmatier is out of the office (returning 09/09/2013) Message-ID: I am out of the office until 09/09/2013. I will be out of the office on September 5th. If you have a question about an order or need assistance placing an order, or if the matter is urgent, please call 877-881-1192 between 8am and 8pm and a VWR Healthcare Service associate will assist you. For quote requests and pricing-related inquiries, I will respond with 24 hours. Thank You, Bruce Palmatier Market Portfolio Manager VWR Healthcare bruce_palmatier@vwr.com mobile: 484.319.5563 fax: 484-881-7307 Customer Service: 877.881.1192 Fax: 484.881.6486 Customer Service email: HEALTHCARESERVICE@VWR.COM Note: This is an automated response to your message "Histonet Digest, Vol 118, Issue 7" sent on 9/6/2013 1:01:07 PM. This is the only notification you will receive while this person is away. From adesupo2002 <@t> hotmail.com Fri Sep 6 19:18:56 2013 From: adesupo2002 <@t> hotmail.com (ADESUPO ADESUYI) Date: Fri Sep 6 19:19:02 2013 Subject: [Histonet] Ki-67 (clone MIB-1) Message-ID: Hi, Please I am interested in pre diluted Ki-67 antibody clone MIB-1 that we can use on our Ventana XT and Ultra. I will really appreciate all the responses that I can get. Thanks, Adesupo Adesuyi Histology Supervisor Norman Regional Health System Norman, OK 73071 From joelleweaver <@t> hotmail.com Sat Sep 7 09:22:14 2013 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Sat Sep 7 09:22:23 2013 Subject: [Histonet] FISH enumeration In-Reply-To: References: <8D0786E6C1AA835-ECC-D72C@Webmail-d108.sysops.aol.com>, Message-ID: For any laboratories out there who perform in house FISH procedures, if you could share what personnel are responsible for doing the signal enumeration & scoring? It would be helpful if you could describe the personnel's training and certification, as well as an approximation of FTE's needed with some volumes. Do you do manual enumeration or use scanning software? Thanks for any input. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: TMcNemar@lmhealth.org > To: thisisann@aol.com; histonet@lists.utsouthwestern.edu > Date: Fri, 6 Sep 2013 05:45:41 -0400 > Subject: RE: [Histonet] Cell Block Preparation > CC: > > This is how we do it now. In the old days, we used agar and to my mind, it is still the best way when you have scant material. > - Spin in a conical tube and pour off > - Melt an agar slant (we get TSA slant from micro) > - Pour the agar into the conical tube and spin for 5 minutes > - The agar will re-solidify and whatever sediment there is will be concentrated in the very tip of the cone > - The agar will slide out of the centrifuge tube > - Slice off the very tip and wrap in lens paper > - Place the wrapped tip in a cassette and process as usual > - Embed the specimen tip down and you are good to go... > > I still use this method today when I feel it necessary. Works great. > > Tom McNemar, HT(ASCP) > Histology Co-ordinator > Licking Memorial Health Systems > (740) 348-4163 > (740) 348-4166 > tmcnemar@lmhealth.org > www.LMHealth.org > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ann Specian > Sent: Thursday, September 05, 2013 12:45 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Cell Block Preparation > > > I am getting complaints in regard to "insufficient" cell blocks. We currently spin, pour off the supernatant, retrieve the sediment and process in lens paper. > > Does anyone have a more current technique which renders better cellularity? > > Also, do you know which renders a better cell block: a fresh specimen, a specimen fixed in Cytolyt or a specimen fixed in 10% NBF? > > Thanks, > Ann > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Sat Sep 7 10:55:10 2013 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sat Sep 7 10:56:26 2013 Subject: AW: [Histonet] FISH enumeration In-Reply-To: References: <8D0786E6C1AA835-ECC-D72C@Webmail-d108.sysops.aol.com>, Message-ID: <000001ceabe2$a208d710$e61a8530$@gmx.at> Pathologists do FISH reading. Learning by doing. Manual enumeration. Each doctor, who orders a FISH has to read it by him/herself. Often they do it with a second person/second look for reliable results. Gudrun Lang Histolab, Linz, Austria -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von joelle weaver Gesendet: Samstag, 07. September 2013 16:22 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] FISH enumeration For any laboratories out there who perform in house FISH procedures, if you could share what personnel are responsible for doing the signal enumeration & scoring? It would be helpful if you could describe the personnel's training and certification, as well as an approximation of FTE's needed with some volumes. Do you do manual enumeration or use scanning software? Thanks for any input. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: TMcNemar@lmhealth.org > To: thisisann@aol.com; histonet@lists.utsouthwestern.edu > Date: Fri, 6 Sep 2013 05:45:41 -0400 > Subject: RE: [Histonet] Cell Block Preparation > CC: > > This is how we do it now. In the old days, we used agar and to my mind, it is still the best way when you have scant material. > - Spin in a conical tube and pour off > - Melt an agar slant (we get TSA slant from micro) > - Pour the agar into the conical tube and spin for 5 minutes > - The agar will re-solidify and whatever sediment there is will be concentrated in the very tip of the cone > - The agar will slide out of the centrifuge tube > - Slice off the very tip and wrap in lens paper > - Place the wrapped tip in a cassette and process as usual > - Embed the specimen tip down and you are good to go... > > I still use this method today when I feel it necessary. Works great. > > Tom McNemar, HT(ASCP) > Histology Co-ordinator > Licking Memorial Health Systems > (740) 348-4163 > (740) 348-4166 > tmcnemar@lmhealth.org > www.LMHealth.org > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ann Specian > Sent: Thursday, September 05, 2013 12:45 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Cell Block Preparation > > > I am getting complaints in regard to "insufficient" cell blocks. We currently spin, pour off the supernatant, retrieve the sediment and process in lens paper. > > Does anyone have a more current technique which renders better cellularity? > > Also, do you know which renders a better cell block: a fresh specimen, a specimen fixed in Cytolyt or a specimen fixed in 10% NBF? > > Thanks, > Ann > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amosbrooks <@t> gmail.com Sat Sep 7 15:57:27 2013 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Sat Sep 7 15:57:35 2013 Subject: [Histonet] Unsubscribe, Chapter 195 Message-ID: Hi, I do not disagree at all. in fact, I think it would make for an interesting management tool. If you have the nicest CV around, but a simple Histonet archive search for your name + "unsubscribe" shows that you can't follow simple instructions, it says something about your communication skills and raises interesting questions about weather you are a good employment candidate. One might also look for the various incarnations of misspelled attempts at unsubscribing such as "unscribe" and "unsiscribe". Just sayin' Amos On Fri, Sep 6, 2013 at 1:00 PM, wrote: > Message: 6 > Date: Fri, 6 Sep 2013 01:56:37 +0000 (UTC) > From: nmhisto@comcast.net > Subject: [Histonet] Unsubscribe, Chapter 195 > To: HISTONET > Message-ID: > < > 1200426006.1029812.1378432597832.JavaMail.root@sz0075a.emeryville.ca.mail.comcast.net > > > > Content-Type: text/plain; charset=utf-8 > > It is a concern that members of our? technically-oriented career field > ? have a difficult time understanding the method for unsubscribing to > Histonet.? There is an ? almost- daily posting to "unsubscribe", despite > the fact that this subject has been addressed literally hundreds of > times.? When one "joins" Histonet, instructions are provided, should be > printed out for reference and used? if the subscriber decides to leave the > group.? We are required to be knowledgeable on all manner of technical > routines requiring detailed instructions and? Histonet is no less clear in > the methods for joining and "un-joining".? Use them, please.? Fire away - > I'm retired and I can take the flak!? I do miss my microtome, though... > From JMacDonald <@t> mtsac.edu Sun Sep 8 03:45:30 2013 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Sun Sep 8 03:45:35 2013 Subject: [Histonet] Help for Northern CA HT program Message-ID: Merritt College has started a HT program and is in need of clinical sites for their students to get "hands-on" experience. If you interested in being a mentor for the HT students please contact Gisele Giorgi at ProfGiorgi@peralta.edu From Richard.Cartun <@t> hhchealth.org Sun Sep 8 19:20:12 2013 From: Richard.Cartun <@t> hhchealth.org (Cartun, Richard) Date: Sun Sep 8 19:20:22 2013 Subject: [Histonet] Cell Block Preparation In-Reply-To: <8D0786E6C1AA835-ECC-D72C@Webmail-d108.sysops.aol.com> References: <8D0786E6C1AA835-ECC-D72C@Webmail-d108.sysops.aol.com> Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E018820D5@HHCEXCHMB05.hhcsystem.org> We borrowed a wonderful cell block procedure from Windham Community Memorial Hospital here in CT several years ago. The specimen is collected fresh in sterile saline or RPMI and then centrifuged to concentrate the cells. Plasma and then thrombin are added to form a clot. The clot is then fixed in formalin and processed in our Histology Laboratory. I will send the procedure to anyone who is interested. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax richard.cartun@hhchealth.org ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Ann Specian [thisisann@aol.com] Sent: Thursday, September 05, 2013 12:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cell Block Preparation I am getting complaints in regard to "insufficient" cell blocks. We currently spin, pour off the supernatant, retrieve the sediment and process in lens paper. Does anyone have a more current technique which renders better cellularity? Also, do you know which renders a better cell block: a fresh specimen, a specimen fixed in Cytolyt or a specimen fixed in 10% NBF? Thanks, Ann _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From talulahgosh <@t> gmail.com Mon Sep 9 06:32:25 2013 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Mon Sep 9 06:32:35 2013 Subject: [Histonet] Unsubscribe, Chapter 195 In-Reply-To: References: Message-ID: Excellent idea!! "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." -Chuck Palahniuk, "Haunted" On Sat, Sep 7, 2013 at 4:57 PM, Amos Brooks wrote: > Hi, > I do not disagree at all. in fact, I think it would make for an > interesting management tool. If you have the nicest CV around, but a simple > Histonet archive search for your name + "unsubscribe" shows that you can't > follow simple instructions, it says something about your communication > skills and raises interesting questions about weather you are a good > employment candidate. One might also look for the various incarnations of > misspelled attempts at unsubscribing such as "unscribe" and "unsiscribe". > > Just sayin' > Amos > > On Fri, Sep 6, 2013 at 1:00 PM, > wrote: > > > Message: 6 > > Date: Fri, 6 Sep 2013 01:56:37 +0000 (UTC) > > From: nmhisto@comcast.net > > Subject: [Histonet] Unsubscribe, Chapter 195 > > To: HISTONET > > Message-ID: > > < > > > 1200426006.1029812.1378432597832.JavaMail.root@sz0075a.emeryville.ca.mail.comcast.net > > > > > > > Content-Type: text/plain; charset=utf-8 > > > > It is a concern that members of our? technically-oriented career field > > ? have a difficult time understanding the method for unsubscribing to > > Histonet.? There is an ? almost- daily posting to "unsubscribe", despite > > the fact that this subject has been addressed literally hundreds of > > times.? When one "joins" Histonet, instructions are provided, should be > > printed out for reference and used? if the subscriber decides to leave > the > > group.? We are required to be knowledgeable on all manner of technical > > routines requiring detailed instructions and? Histonet is no less clear > in > > the methods for joining and "un-joining".? Use them, please.? Fire > away - > > I'm retired and I can take the flak!? I do miss my microtome, though... > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Erin.Martin <@t> ucsf.edu Mon Sep 9 08:28:29 2013 From: Erin.Martin <@t> ucsf.edu (Martin, Erin) Date: Mon Sep 9 08:28:43 2013 Subject: [Histonet] (no subject) Message-ID: <24B7B291CC88D04AB663958E77A1F59D18144C@ex09.net.ucsf.edu> Good morning all! One of our fellows emailed me a question that she came across while studying for her boards: "I'm studying for my board exam and came across questions re: paraffin embedding. It reads: best temperature for paraffin embedding is 38-48 48-58 58-70. I am getting some info on Internet that says 58 but is the range lower or higher than that? What do we use?" This seems to me to be an odd question because it depends on the melting point of the paraffin in use. Ours melts at 58C and we embed at 60C, but we have also used paraffin that melts at 56C and we embedded at 58C. Or am I missing something? Does anyone have a clear cut answer to this? Thanks everyone! Erin Erin Martin, Histology Supervisor UCSF Dermatopathology Service 415-353-7248 Confidentiality Notice The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential and/or priviledged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited. If you receive this in error, please contact the sender and delete the material from any computer. From philip_manfre <@t> merck.com Mon Sep 9 08:42:48 2013 From: philip_manfre <@t> merck.com (Manfre, Philip) Date: Mon Sep 9 08:42:56 2013 Subject: [Histonet] (no subject) In-Reply-To: <24B7B291CC88D04AB663958E77A1F59D18144C@ex09.net.ucsf.edu> References: <24B7B291CC88D04AB663958E77A1F59D18144C@ex09.net.ucsf.edu> Message-ID: <558A4571351D0C42BD923F403F4198C4B2701AE678@USCTMXP51014.merck.com> It's a bad question. Generally the "best" temperature is probably between 55 and 65, depending on the paraffin. This would fall between the two latter answers. Hmm.... What to do? Phil. Philip Manfre, B.A., HT (ASCP) Associate Principal Scientist Merck Research Laboratories WP45-251 PO Box 4 West Point, PA 19486 215-652-9750 215-993-0383 (fax) philip_manfre@merck.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martin, Erin Sent: Monday, September 09, 2013 9:28 AM To: histonet Cc: Naujokas, Agne; Meier, Melissa Subject: [Histonet] (no subject) Good morning all! One of our fellows emailed me a question that she came across while studying for her boards: "I'm studying for my board exam and came across questions re: paraffin embedding. It reads: best temperature for paraffin embedding is 38-48 48-58 58-70. I am getting some info on Internet that says 58 but is the range lower or higher than that? What do we use?" This seems to me to be an odd question because it depends on the melting point of the paraffin in use. Ours melts at 58C and we embed at 60C, but we have also used paraffin that melts at 56C and we embedded at 58C. Or am I missing something? Does anyone have a clear cut answer to this? Thanks everyone! Erin Erin Martin, Histology Supervisor UCSF Dermatopathology Service 415-353-7248 Confidentiality Notice The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential and/or priviledged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited. If you receive this in error, please contact the sender and delete the material from any computer. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From PAMarcum <@t> uams.edu Mon Sep 9 08:50:06 2013 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Mon Sep 9 08:50:10 2013 Subject: [Histonet] (no subject) In-Reply-To: <24B7B291CC88D04AB663958E77A1F59D18144C@ex09.net.ucsf.edu> References: <24B7B291CC88D04AB663958E77A1F59D18144C@ex09.net.ucsf.edu> Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA32D3157DDE@Mail2Node2.ad.uams.edu> The last answer will cover most paraffins used routinely and may cover microwave also. Temperatures below 58C are often melt points however; they may not be a complete liquid or have slightly increased viscosity. Pam Marcum UAMS -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martin, Erin Sent: Monday, September 09, 2013 8:28 AM To: histonet Cc: Naujokas, Agne; Meier, Melissa Subject: [Histonet] (no subject) Good morning all! One of our fellows emailed me a question that she came across while studying for her boards: "I'm studying for my board exam and came across questions re: paraffin embedding. It reads: best temperature for paraffin embedding is 38-48 48-58 58-70. I am getting some info on Internet that says 58 but is the range lower or higher than that? What do we use?" This seems to me to be an odd question because it depends on the melting point of the paraffin in use. Ours melts at 58C and we embed at 60C, but we have also used paraffin that melts at 56C and we embedded at 58C. Or am I missing something? Does anyone have a clear cut answer to this? Thanks everyone! Erin Erin Martin, Histology Supervisor UCSF Dermatopathology Service 415-353-7248 Confidentiality Notice The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential and/or priviledged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited. If you receive this in error, please contact the sender and delete the material from any computer. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From relia1 <@t> earthlink.net Mon Sep 9 09:03:12 2013 From: relia1 <@t> earthlink.net (Pam Barker) Date: Mon Sep 9 09:03:19 2013 Subject: [Histonet] RELIA Hot Histology Job Alert Histology Supervisor needed in North Florida. Message-ID: <003f01cead65$52b52b30$f81f8190$@earthlink.net> Hi Histonetters!! I hope you all had a great weekend!! And I hope your teams NCAA and NFL did better than mine did!! I wanted to take a minute and tell you about a position I recently was engaged on that I am very excited about. RELIA has been engaged to recruit for a histology supervisor for a private pathology lab located in North Florida. This is a busy full staffed private full service pathology lab which processes all types of tissue and runs a full menu of special stains and IHC. The ideal candidate for my client will have at least 5 years of supervisory experience in a histology lab. HT/HTL and Florida Supervisor license or elig are required. My client offers an excellent salary, benefits and relocation assistance. For more information please contact me - Pam Barker toll free at 866-607-3542 until 5pm EST or anytime on my cell phone at 407-353-5070 or via email at relia1@earthlink.net to set up a time to talk. If you know of someone who might be interested remember RELIA offers a referral bonus. If we place someone you refer you will earn a referral bonus. Have a great Monday!!! Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From lpwenk <@t> sbcglobal.net Mon Sep 9 09:47:55 2013 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Mon Sep 9 09:48:04 2013 Subject: [Histonet] Unsubscribe, Chapter 195 In-Reply-To: <558A4571351D0C42BD923F403F4198C4B135BC24F9@USCTMXP51014.merck.com> References: <1755295187.1029338.1378431833151.JavaMail.root@sz0075a.emeryville.ca.mail.comcast.net><1200426006.1029812.1378432597832.JavaMail.root@sz0075a.emeryville.ca.mail.comcast.net> <558A4571351D0C42BD923F403F4198C4B135BC24F9@USCTMXP51014.merck.com> Message-ID: I'm going to wade in, not as someone who has posted numerous times on how to unsubscribe, but as someone assessing it from a "risk assessment" evaluation. If there is a lab task that is consistently being done wrong, by many different people, it is usually NOT the fault of the people. It is either a training issue, or a process problem. So we either have to do a better job training and re-training, or we need to change how the process/procedure is being done. With the Histonet email, since people are constantly joining, often for a day or two, we can't really improve the "training" aspect. Yes, there are instructions when we first join, to print off/save how to subscribe or unsubscribe or change personal information, etc. But (be honest) how many of us pay attention to these types of instructions when we sign up to be a "member" of a credit card or a on-line department store or an on-line book store or other email lists? Most people do not. So we know that this type of "training" is not effective. But we really can't do a one-on-one type of training session for each person who signs on to Histonet. Therefore, improving the training is not the answer. The answer lies in modifying the process. Look at the bottom of those emails from credit cards or hotels or department stores that you have signed up with. There is usually a line that says "If you no longer wish to receive these emails, click on this link and follow the instructions". Add to that, various email lists have various methods on how to unsubscribe, which can involve a link, or putting the word unsubscribe in the subject, or putting the word unsubscribe in the message. Histonet has a link at the bottom, but no instructions. So it's not clear to click on the link to unsubscribe, nor is there any mention whether one of the other unsubscribing methods would work. I therefore believe the Histonet unsubscribing procedure has a process problem, that could be easily fixed. As for the fact that how to unsubscribe has been explained 5,391+ times in the past does not help the person who signed up over the weekend, and as of today, decided that Histonet is not what they need. This new person has not seen the previous requests for help with unsubscribing, nor the answers on how to do it. Again, this is a process problem. Is there any way Histonet can get some clearer instructions at the bottom of each email, on how to unsubscribe, either permanently or temporarily while on vacation? Such as saying "To unsubscribe, click on the link below, and follow the instructions at the bottom of the next webpage". Let's not yell at the people trying to unsubscribe. Let's work on improving the unsubscribing process, so we don't get these requests. Peggy A. Wenk, HTL(ASCP)SLS -----Original Message----- From: Manfre, Philip Sent: Friday, September 06, 2013 7:30 AM To: nmhisto@comcast.net ; HISTONET Subject: RE: [Histonet] Unsubscribe, Chapter 195 Trained professionals should know by now that if you want to unsubscribe, you must type in all caps - UNSUBSCRIBE Philip Manfre, B.A., HT (ASCP) Associate Principal Scientist Merck Research Laboratories WP45-251 PO Box 4 West Point, PA 19486 215-652-9750 215-993-0383 (fax) philip_manfre@merck.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of nmhisto@comcast.net Sent: Thursday, September 05, 2013 9:57 PM To: HISTONET Subject: [Histonet] Unsubscribe, Chapter 195 It is a concern that members of our technically-oriented career field have a difficult time understanding the method for unsubscribing to Histonet. There is an almost- daily posting to "unsubscribe", despite the fact that this subject has been addressed literally hundreds of times. When one "joins" Histonet, instructions are provided, should be printed out for reference and used if the subscriber decides to leave the group. We are required to be knowledgeable on all manner of technical routines requiring detailed instructions and Histonet is no less clear in the methods for joining and "un-joining". Use them, please. Fire away - I'm retired and I can take the flak! I do miss my microtome, though... _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nmhisto <@t> comcast.net Mon Sep 9 09:58:09 2013 From: nmhisto <@t> comcast.net (nmhisto@comcast.net) Date: Mon Sep 9 09:58:41 2013 Subject: [Histonet] Unsubscribe, Chapter 195 In-Reply-To: Message-ID: <670394625.1111582.1378738689536.JavaMail.root@sz0075a.emeryville.ca.mail.comcast.net> I?was happy to read your thoughtful evaluation of the problem and, after having read through it, wonder why I hadn't thought of it before!? Perhaps a little "tweaking" by the listserv management would make this process much more clear.? The sign-on page of information about Histonet is rather lengthy (rightly so) but perhaps it's time for a little updating.? Thank you, Peggy, for your thoughts and proposed solution. ----- Original Message ----- From: "Lee & Peggy Wenk" To: "Philip Manfre" , nmhisto@comcast.net, "HISTONET" Sent: Monday, September 9, 2013 8:47:55 AM Subject: Re: [Histonet] Unsubscribe, Chapter 195 I'm going to wade in, not as someone who has posted numerous times on how to unsubscribe, but as someone assessing it from a "risk assessment" evaluation. If there is a lab task that is consistently being done wrong, by many different people, it is usually NOT the fault of the people. It is either a training issue, or a process problem. So we either have to do a better job training and re-training, or we need to change how the process/procedure is being done. With the Histonet email, since people are constantly joining, often for a day or two, we can't really improve the "training" aspect. Yes, there are instructions when we first join, to print off/save how to subscribe or unsubscribe or change personal information, etc. But (be honest) how many of us pay attention to these types of instructions when we sign up to be a "member" of a credit card or a on-line department store or an on-line book store or other email lists? Most people do not. So we know that this type of "training" is not effective. But we really can't do a one-on-one type of training session for each person who signs on to Histonet. Therefore, improving the training is not the answer. The answer lies in modifying the process. Look at the bottom of those emails from credit cards or hotels or department stores that you have signed up with. There is usually a line that says "If you no longer wish to receive these emails, click on this link and follow the instructions". Add to that, various email lists have various methods on how to unsubscribe, which can involve a link, or putting the word unsubscribe in the subject, or putting the word unsubscribe in the message. Histonet has a link at the bottom, but no instructions. So it's not clear to click on the link to unsubscribe, nor is there any mention whether one of the other unsubscribing methods would work. I therefore believe the Histonet unsubscribing procedure has a process problem, that could be easily fixed. As for the fact that how to unsubscribe has been explained 5,391+ times in the past does not help the person who signed up over the weekend, and as of today, decided that Histonet is not what they need. This new person has not seen the previous requests for help with unsubscribing, nor the answers on how to do it. Again, this is a process problem. Is there any way Histonet can get some clearer instructions at the bottom of each email, on how to unsubscribe, either permanently or temporarily while on vacation? Such as saying "To unsubscribe, click on the link below, and follow the instructions at the bottom of the next webpage". Let's not yell at the people trying to unsubscribe. Let's work on improving the unsubscribing process, so we don't get these requests. Peggy A. Wenk, HTL(ASCP)SLS -----Original Message----- From: Manfre, Philip Sent: Friday, September 06, 2013 7:30 AM To: nmhisto@comcast.net ; HISTONET Subject: RE: [Histonet] Unsubscribe, Chapter 195 Trained professionals should know by now that if you want to unsubscribe, you must type in all caps - UNSUBSCRIBE Philip Manfre, B.A., HT (ASCP) Associate Principal Scientist Merck Research Laboratories WP45-251 PO Box 4 West Point, PA 19486 215-652-9750 215-993-0383 (fax) philip_manfre@merck.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of nmhisto@comcast.net Sent: Thursday, September 05, 2013 9:57 PM To: HISTONET Subject: [Histonet] Unsubscribe, Chapter 195 It is a concern that members of our ?technically-oriented career field ?have a difficult time understanding the method for unsubscribing to Histonet. There is an ?almost- daily posting to "unsubscribe", despite the fact that this subject has been addressed literally hundreds of times. ?When one "joins" Histonet, instructions are provided, should be printed out for reference and used if the subscriber decides to leave the group. ?We are required to be knowledgeable on all manner of technical routines requiring detailed instructions and ?Histonet is no less clear in the methods for joining and "un-joining". ?Use them, please. ?Fire away - I'm retired and I can take the flak! ?I do miss my microtome, though... _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: ?This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sforeman <@t> labpath.com Mon Sep 9 10:18:23 2013 From: sforeman <@t> labpath.com (Susan Foreman) Date: Mon Sep 9 10:25:05 2013 Subject: [Histonet] ICC on previously Wright stained smears Message-ID: <002101cead6f$d3a40540$7aec0fc0$@com> Is anyone running ICC on previously Wright-Giemsa stained smears? What kind of info can you offer? We didn't have any staining without antigen retrieval, so we added that step. So with the antigen retrieval, the cells are in good shape, but now there is too much background without very much cellular staining. What recommendations do you have? Longer or shorter time in antigen retrieval? Adjust the pH of the antigen retrieval? We don't have very many smears left on this research patient. Many Thanks, Susan From rjbuesa <@t> yahoo.com Mon Sep 9 10:28:47 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Sep 9 10:28:53 2013 Subject: [Histonet] ICC on previously Wright stained smears In-Reply-To: <002101cead6f$d3a40540$7aec0fc0$@com> References: <002101cead6f$d3a40540$7aec0fc0$@com> Message-ID: <1378740527.86695.YahooMailNeo@web163106.mail.bf1.yahoo.com> You need antigen retrieval if you have a specimen that has been fixed with NBF but usually blood smears are just fixed with methanol and in this case antigen retrieval is not a necessary step. Eliminate it and you will probably will get better results altogether. Ren? J. ________________________________ From: Susan Foreman To: histonet@lists.utsouthwestern.edu Sent: Monday, September 9, 2013 11:18 AM Subject: [Histonet] ICC on previously Wright stained smears Is anyone running ICC on previously Wright-Giemsa stained smears?? What kind of info can you offer?? We didn't have any staining without antigen retrieval, so we added that step.? So with the antigen retrieval,? the cells are in good shape, but now there is too much background without very much cellular staining.? What recommendations do you have?? Longer or shorter time in antigen retrieval?? Adjust the pH of the antigen retrieval?? We don't have very many smears left on this research patient. Many Thanks, Susan _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dunatrsd <@t> sbcglobal.net Mon Sep 9 10:46:29 2013 From: dunatrsd <@t> sbcglobal.net (dusko trajkovic) Date: Mon Sep 9 10:46:35 2013 Subject: [Histonet] Unsubscribe, Chapter 195 In-Reply-To: References: <1755295187.1029338.1378431833151.JavaMail.root@sz0075a.emeryville.ca.mail.comcast.net><1200426006.1029812.1378432597832.JavaMail.root@sz0075a.emeryville.ca.mail.comcast.net> <558A4571351D0C42BD923F403F4198C4B135BC24F9@USCTMXP51014.merck.com> Message-ID: <1378741589.46291.YahooMailNeo@web181705.mail.ne1.yahoo.com> Peggy, I don't think anyone else could have said it better. Thank you Dusko ________________________________ From: Lee & Peggy Wenk To: "Manfre, Philip" ; nmhisto@comcast.net; HISTONET Sent: Monday, September 9, 2013 7:47 AM Subject: Re: [Histonet] Unsubscribe, Chapter 195 I'm going to wade in, not as someone who has posted numerous times on how to unsubscribe, but as someone assessing it from a "risk assessment" evaluation. If there is a lab task that is consistently being done wrong, by many different people, it is usually NOT the fault of the people. It is either a training issue, or a process problem. So we either have to do a better job training and re-training, or we need to change how the process/procedure is being done. With the Histonet email, since people are constantly joining, often for a day or two, we can't really improve the "training" aspect. Yes, there are instructions when we first join, to print off/save how to subscribe or unsubscribe or change personal information, etc. But (be honest) how many of us pay attention to these types of instructions when we sign up to be a "member" of a credit card or a on-line department store or an on-line book store or other email lists? Most people do not. So we know that this type of "training" is not effective. But we really can't do a one-on-one type of training session for each person who signs on to Histonet. Therefore, improving the training is not the answer. The answer lies in modifying the process. Look at the bottom of those emails from credit cards or hotels or department stores that you have signed up with. There is usually a line that says "If you no longer wish to receive these emails, click on this link and follow the instructions". Add to that, various email lists have various methods on how to unsubscribe, which can involve a link, or putting the word unsubscribe in the subject, or putting the word unsubscribe in the message. Histonet has a link at the bottom, but no instructions. So it's not clear to click on the link to unsubscribe, nor is there any mention whether one of the other unsubscribing methods would work. I therefore believe the Histonet unsubscribing procedure has a process problem, that could be easily fixed. As for the fact that how to unsubscribe has been explained 5,391+ times in the past does not help the person who signed up over the weekend, and as of today, decided that Histonet is not what they need. This new person has not seen the previous requests for help with unsubscribing, nor the answers on how to do it. Again, this is a process problem. Is there any way Histonet can get some clearer instructions at the bottom of each email, on how to unsubscribe, either permanently or temporarily while on vacation? Such as saying "To unsubscribe, click on the link below, and follow the instructions at the bottom of the next webpage". Let's not yell at the people trying to unsubscribe. Let's work on improving the unsubscribing process, so we don't get these requests. Peggy A. Wenk, HTL(ASCP)SLS -----Original Message----- From: Manfre, Philip Sent: Friday, September 06, 2013 7:30 AM To: nmhisto@comcast.net ; HISTONET Subject: RE: [Histonet] Unsubscribe, Chapter 195 Trained professionals should know by now that if you want to unsubscribe, you must type in all caps - UNSUBSCRIBE Philip Manfre, B.A., HT (ASCP) Associate Principal Scientist Merck Research Laboratories WP45-251 PO Box 4 West Point, PA 19486 215-652-9750 215-993-0383 (fax) philip_manfre@merck.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of nmhisto@comcast.net Sent: Thursday, September 05, 2013 9:57 PM To: HISTONET Subject: [Histonet] Unsubscribe, Chapter 195 It is a concern that members of our? technically-oriented career field? have a difficult time understanding the method for unsubscribing to Histonet. There is an? almost- daily posting to "unsubscribe", despite the fact that this subject has been addressed literally hundreds of times.? When one "joins" Histonet, instructions are provided, should be printed out for reference and used if the subscriber decides to leave the group.? We are required to be knowledgeable on all manner of technical routines requiring detailed instructions and? Histonet is no less clear in the methods for joining and "un-joining".? Use them, please.? Fire away - I'm retired and I can take the flak!? I do miss my microtome, though... _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice:? This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Mon Sep 9 11:26:38 2013 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Mon Sep 9 11:26:46 2013 Subject: [Histonet] (no subject) In-Reply-To: <24B7B291CC88D04AB663958E77A1F59D18144C@ex09.net.ucsf.edu> References: <24B7B291CC88D04AB663958E77A1F59D18144C@ex09.net.ucsf.edu> Message-ID: Recommended melting point of paraffin is 2-4 degrees above the melting point of the paraffin. Because we really don't see paraffins that would have a melting point of 46, the BEST answer would be 58-70. Perhaps not what we do, but the best answer for the choices provided. From: "Martin, Erin" To: histonet Cc: "Naujokas, Agne" , "Meier, Melissa" Date: 09/09/2013 06:30 AM Subject: [Histonet] (no subject) Sent by: histonet-bounces@lists.utsouthwestern.edu Good morning all! One of our fellows emailed me a question that she came across while studying for her boards: "I'm studying for my board exam and came across questions re: paraffin embedding. It reads: best temperature for paraffin embedding is 38-48 48-58 58-70. I am getting some info on Internet that says 58 but is the range lower or higher than that? What do we use?" This seems to me to be an odd question because it depends on the melting point of the paraffin in use. Ours melts at 58C and we embed at 60C, but we have also used paraffin that melts at 56C and we embedded at 58C. Or am I missing something? Does anyone have a clear cut answer to this? Thanks everyone! Erin Erin Martin, Histology Supervisor UCSF Dermatopathology Service 415-353-7248 Confidentiality Notice The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential and/or priviledged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited. If you receive this in error, please contact the sender and delete the material from any computer. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SHUNTER <@t> beaumont.edu Mon Sep 9 11:59:18 2013 From: SHUNTER <@t> beaumont.edu (Sue Hunter) Date: Mon Sep 9 11:59:24 2013 Subject: [Histonet] FISH enumeration In-Reply-To: References: <8D0786E6C1AA835-ECC-D72C@Webmail-d108.sysops.aol.com>, Message-ID: My techs do the scoring for the FISH testing. We have a Leica Ariol system - the HER2 FISH are computer assisted, but the Urovysions are manually assessed. That may change with the new systems we are receiving. My techs are exceptionally well trained in morphology - they sit with our director to learn, and are really really good. We do about 10 urovysions per week and sometimes as many as 30 pathvysions per week. I have one or two techs on the FISH rotation each week. The other techs step in as needed. The pathologist circles the area on the HER2 IHC slide to be FISHed. The Pathologists do come down to the lab to see cases, but they rely on the techs. The pathologists also look at all other criteria before signing out the case to make sure everything fits. We are also getting a new image hub so the saved images will be viewable by the pathologists - this will be a really nice addition to the reviewing process. I believe there are many laboratories where the cytotechs read out the urovsyions. Sue Sue Hunter, Supervisor Advanced Diagnostics Beaumont Health System Royal Oak MI 248-898-5146 shunter@beaumont.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelle weaver Sent: Saturday, September 07, 2013 10:22 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FISH enumeration For any laboratories out there who perform in house FISH procedures, if you could share what personnel are responsible for doing the signal enumeration & scoring? It would be helpful if you could describe the personnel's training and certification, as well as an approximation of FTE's needed with some volumes. Do you do manual enumeration or use scanning software? Thanks for any input. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: TMcNemar@lmhealth.org > To: thisisann@aol.com; histonet@lists.utsouthwestern.edu > Date: Fri, 6 Sep 2013 05:45:41 -0400 > Subject: RE: [Histonet] Cell Block Preparation > CC: > > This is how we do it now. In the old days, we used agar and to my mind, it is still the best way when you have scant material. > - Spin in a conical tube and pour off > - Melt an agar slant (we get TSA slant from micro) > - Pour the agar into the conical tube and spin for 5 minutes > - The agar will re-solidify and whatever sediment there is will be concentrated in the very tip of the cone > - The agar will slide out of the centrifuge tube > - Slice off the very tip and wrap in lens paper > - Place the wrapped tip in a cassette and process as usual > - Embed the specimen tip down and you are good to go... > > I still use this method today when I feel it necessary. Works great. > > Tom McNemar, HT(ASCP) > Histology Co-ordinator > Licking Memorial Health Systems > (740) 348-4163 > (740) 348-4166 > tmcnemar@lmhealth.org > www.LMHealth.org > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ann Specian > Sent: Thursday, September 05, 2013 12:45 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Cell Block Preparation > > > I am getting complaints in regard to "insufficient" cell blocks. We currently spin, pour off the supernatant, retrieve the sediment and process in lens paper. > > Does anyone have a more current technique which renders better cellularity? > > Also, do you know which renders a better cell block: a fresh specimen, a specimen fixed in Cytolyt or a specimen fixed in 10% NBF? > > Thanks, > Ann > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TJohnson <@t> gnf.org Mon Sep 9 12:12:48 2013 From: TJohnson <@t> gnf.org (Teri Johnson) Date: Mon Sep 9 12:12:54 2013 Subject: [Histonet] Re: ICC on previously Wright stained smears Message-ID: <9F3CFEE76E51B64991C7485270890B40497C0A34@EX5.lj.gnf.org> Hi Susan, If you are getting background with little cellular staining, then the antigen retrieval method isn't working. Rene is correct that the smears you are using were most likely methanol fixed. Although heat induced epitope retrieval is thought to be most useful for formalin fixed specimens, there is also some benefit to using heat to denature (reconfigure) some of the proteins and you may get good staining in minimally fixed or alternatively fixed material. I have had success using it in minimally fixed frozen sections, when standard dogma says it shouldn't be necessary (no cross-linking occurred). Your biggest problem may be that the antibody you are using does not recognize an alcohol-fixed protein, and no amount of antigen retrieval can fix that. Teri Johnson Manager, Histology Genomics Institute for Novartis Research Foundation San Diego, CA 858-332-4752 From leila.etemadi <@t> med.lu.se Mon Sep 9 12:27:06 2013 From: leila.etemadi <@t> med.lu.se (Leila Etemadi) Date: Mon Sep 9 12:27:14 2013 Subject: [Histonet] VIP antibody Message-ID: <7B81E779-146C-4D3D-BF09-2D8BDC202DC7@med.lu.se> Hello every body, I wounded if any of you is working with VIP antibody ( rabbit)?, Any advice for selecting right supplier will be appreciated. The best of this antibody used to be presented by Eurodiagnostica, which unfortunately they are not presenting it any more for IHC project ( they just sell the kit now a days). Santa cruz, milipore?.?!?, Any suggestion?!? Many thanks, Leila :-) From CJacobs <@t> clinpath.com Mon Sep 9 12:30:13 2013 From: CJacobs <@t> clinpath.com (Christopher Jacobs) Date: Mon Sep 9 12:30:17 2013 Subject: [Histonet] Travel Histology Technician Jobs Message-ID: <0082E20E79ED2245AB1DD78B87CFA1094912C9A4@cpd-exchange.ClinPath.local> Histonetters, Does anyone out there have any information on traveling histology technician jobs? What is the compensation? How does insurance work? What qualifications are needed? I am particularly interested in any personal experiences any one has had. Thanks! "CJ" Christopher P. Jacobs, HT QIHC(ASCP) Clin-Path Diagnostics, LLC CONFIDENTIALITY NOTICE: This message and any attachments are for the sole use of the intended recipient(s). This message is confidential and may also be privileged. If you are not the intended recipient, please contact the sender immediately, delete the contents of this message and do not use it for any purpose. From gkerce <@t> uga.edu Mon Sep 9 13:15:19 2013 From: gkerce <@t> uga.edu (Gwindolyn W Kerce) Date: Mon Sep 9 13:15:36 2013 Subject: [Histonet] IHC Stainer Repair Message-ID: <2278E076D598F14BAB50EE8EAD35EA826DB3E267@BLUPRD0210MB354.namprd02.prod.outlook.com> I am looking for someone to work on my Dako Autostainer and my Leica ST5050 stainer in the Atlanta area. Can anyone recommend someone? Thanks, Gwin Kerce ASCP(HT) laboratory supervisor University of Georgia College of Veterinary Medicine Dept of Population Health Poultry Diagnostic and Research Center Histopathology laboratory rm 244 953 College Station Rd. Athens, GA 30605 (706) 583-0636 From lpwenk <@t> sbcglobal.net Mon Sep 9 14:00:22 2013 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Mon Sep 9 14:00:32 2013 Subject: [Histonet] Travel Histology Technician Jobs In-Reply-To: <0082E20E79ED2245AB1DD78B87CFA1094912C9A4@cpd-exchange.ClinPath.local> References: <0082E20E79ED2245AB1DD78B87CFA1094912C9A4@cpd-exchange.ClinPath.local> Message-ID: <6DB0AEDB1C984207823DB5DFA9BBC3E0@HP2010> On Sunday, Sept. 22, from 8 am - 9:30 am at the NSH Symposium in Providence, RI, Beth Cox, HTL/SCT(ASCP)QIHC is presenting a workshop on "Work and Play Across the USA - A Guide to Being a Traveling Tech". http://www.histoconvention.org/ Click on Schedule Peggy A. Wenk, HTL(ASCP)SLS -----Original Message----- From: Christopher Jacobs Sent: Monday, September 09, 2013 1:30 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Travel Histology Technician Jobs Histonetters, Does anyone out there have any information on traveling histology technician jobs? What is the compensation? How does insurance work? What qualifications are needed? I am particularly interested in any personal experiences any one has had. Thanks! "CJ" Christopher P. Jacobs, HT QIHC(ASCP) Clin-Path Diagnostics, LLC CONFIDENTIALITY NOTICE: This message and any attachments are for the sole use of the intended recipient(s). This message is confidential and may also be privileged. If you are not the intended recipient, please contact the sender immediately, delete the contents of this message and do not use it for any purpose. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JSilverman <@t> NSHS.edu Mon Sep 9 14:08:23 2013 From: JSilverman <@t> NSHS.edu (Silverman, Jeffrey) Date: Mon Sep 9 14:08:37 2013 Subject: [Histonet] unsubscribe In-Reply-To: References: Message-ID: <397232384CF7F447AAEF570F87F5A4560179B0B6890B@SYKECHXVS01.nslijhs.net> Jeffrey S. Silverman HT HTL QIHC (ASCP) Operations Manager Anatomic Pathology Specimen Processing, Laboratory Safety Officer Lenox Hill Hospital 12 Uris- Anatomic Pathology 100 East 77th Street New York, New York 10075 office- 212 434 6673 cell-631 375 4505 email: jsilverman@nshs.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Monday, September 09, 2013 1:03 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 118, Issue 10 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: Cell Block Preparation (Cartun, Richard) 2. Re: Unsubscribe, Chapter 195 (Emily Sours) 3. (no subject) (Martin, Erin) 4. RE: (no subject) (Manfre, Philip) 5. RE: (no subject) (Marcum, Pamela A) 6. RELIA Hot Histology Job Alert Histology Supervisor needed in North Florida. (Pam Barker) 7. Re: Unsubscribe, Chapter 195 (Lee & Peggy Wenk) 8. Re: Unsubscribe, Chapter 195 (nmhisto@comcast.net) 9. ICC on previously Wright stained smears (Susan Foreman) 10. Re: ICC on previously Wright stained smears (Rene J Buesa) 11. Re: Unsubscribe, Chapter 195 (dusko trajkovic) 12. Re: (no subject) (Jennifer MacDonald) 13. RE: FISH enumeration (Sue Hunter) ---------------------------------------------------------------------- Message: 1 Date: Mon, 9 Sep 2013 00:20:12 +0000 From: "Cartun, Richard" Subject: RE: [Histonet] Cell Block Preparation To: Ann Specian , "histonet@lists.utsouthwestern.edu" Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E018820D5@HHCEXCHMB05.hhcsystem.org> Content-Type: text/plain; charset="us-ascii" We borrowed a wonderful cell block procedure from Windham Community Memorial Hospital here in CT several years ago. The specimen is collected fresh in sterile saline or RPMI and then centrifuged to concentrate the cells. Plasma and then thrombin are added to form a clot. The clot is then fixed in formalin and processed in our Histology Laboratory. I will send the procedure to anyone who is interested. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax richard.cartun@hhchealth.org ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Ann Specian [thisisann@aol.com] Sent: Thursday, September 05, 2013 12:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cell Block Preparation I am getting complaints in regard to "insufficient" cell blocks. We currently spin, pour off the supernatant, retrieve the sediment and process in lens paper. Does anyone have a more current technique which renders better cellularity? Also, do you know which renders a better cell block: a fresh specimen, a specimen fixed in Cytolyt or a specimen fixed in 10% NBF? Thanks, Ann _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. ------------------------------ Message: 2 Date: Mon, 9 Sep 2013 07:32:25 -0400 From: Emily Sours Subject: Re: [Histonet] Unsubscribe, Chapter 195 To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=UTF-8 Excellent idea!! "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." -Chuck Palahniuk, "Haunted" On Sat, Sep 7, 2013 at 4:57 PM, Amos Brooks wrote: > Hi, > I do not disagree at all. in fact, I think it would make for an > interesting management tool. If you have the nicest CV around, but a simple > Histonet archive search for your name + "unsubscribe" shows that you can't > follow simple instructions, it says something about your communication > skills and raises interesting questions about weather you are a good > employment candidate. One might also look for the various incarnations of > misspelled attempts at unsubscribing such as "unscribe" and "unsiscribe". > > Just sayin' > Amos > > On Fri, Sep 6, 2013 at 1:00 PM, > wrote: > > > Message: 6 > > Date: Fri, 6 Sep 2013 01:56:37 +0000 (UTC) > > From: nmhisto@comcast.net > > Subject: [Histonet] Unsubscribe, Chapter 195 > > To: HISTONET > > Message-ID: > > < > > > 1200426006.1029812.1378432597832.JavaMail.root@sz0075a.emeryville.ca.mail.comcast.net > > > > > > > Content-Type: text/plain; charset=utf-8 > > > > It is a concern that members of our?? technically-oriented career field > > ?? have a difficult time understanding the method for unsubscribing to > > Histonet.?? There is an ?? almost- daily posting to "unsubscribe", despite > > the fact that this subject has been addressed literally hundreds of > > times.?? When one "joins" Histonet, instructions are provided, should be > > printed out for reference and used?? if the subscriber decides to leave > the > > group.?? We are required to be knowledgeable on all manner of technical > > routines requiring detailed instructions and?? Histonet is no less clear > in > > the methods for joining and "un-joining".?? Use them, please.?? Fire > away - > > I'm retired and I can take the flak!?? I do miss my microtome, though... > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 3 Date: Mon, 9 Sep 2013 13:28:29 +0000 From: "Martin, Erin" Subject: [Histonet] (no subject) To: histonet Cc: "Naujokas, Agne" , "Meier, Melissa" Message-ID: <24B7B291CC88D04AB663958E77A1F59D18144C@ex09.net.ucsf.edu> Content-Type: text/plain; charset=iso-8859-1 Good morning all! One of our fellows emailed me a question that she came across while studying for her boards: "I'm studying for my board exam and came across questions re: paraffin embedding. It reads: best temperature for paraffin embedding is 38-48 48-58 58-70. I am getting some info on Internet that says 58 but is the range lower or higher than that? What do we use?" This seems to me to be an odd question because it depends on the melting point of the paraffin in use. Ours melts at 58C and we embed at 60C, but we have also used paraffin that melts at 56C and we embedded at 58C. Or am I missing something? Does anyone have a clear cut answer to this? Thanks everyone! Erin Erin Martin, Histology Supervisor UCSF Dermatopathology Service 415-353-7248 Confidentiality Notice The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential and/or priviledged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited. If you receive this in error, please contact the sender and delete the material from any computer. ------------------------------ Message: 4 Date: Mon, 9 Sep 2013 09:42:48 -0400 From: "Manfre, Philip" Subject: RE: [Histonet] (no subject) To: "Martin, Erin" , histonet Cc: "Naujokas, Agne" , "Meier, Melissa" Message-ID: <558A4571351D0C42BD923F403F4198C4B2701AE678@USCTMXP51014.merck.com> Content-Type: text/plain; charset="us-ascii" It's a bad question. Generally the "best" temperature is probably between 55 and 65, depending on the paraffin. This would fall between the two latter answers. Hmm.... What to do? Phil. Philip Manfre, B.A., HT (ASCP) Associate Principal Scientist Merck Research Laboratories WP45-251 PO Box 4 West Point, PA 19486 215-652-9750 215-993-0383 (fax) philip_manfre@merck.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martin, Erin Sent: Monday, September 09, 2013 9:28 AM To: histonet Cc: Naujokas, Agne; Meier, Melissa Subject: [Histonet] (no subject) Good morning all! One of our fellows emailed me a question that she came across while studying for her boards: "I'm studying for my board exam and came across questions re: paraffin embedding. It reads: best temperature for paraffin embedding is 38-48 48-58 58-70. I am getting some info on Internet that says 58 but is the range lower or higher than that? What do we use?" This seems to me to be an odd question because it depends on the melting point of the paraffin in use. Ours melts at 58C and we embed at 60C, but we have also used paraffin that melts at 56C and we embedded at 58C. Or am I missing something? Does anyone have a clear cut answer to this? Thanks everyone! Erin Erin Martin, Histology Supervisor UCSF Dermatopathology Service 415-353-7248 Confidentiality Notice The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential and/or priviledged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited. If you receive this in error, please contact the sender and delete the material from any computer. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------ Message: 5 Date: Mon, 9 Sep 2013 13:50:06 +0000 From: "Marcum, Pamela A" Subject: RE: [Histonet] (no subject) To: "'Martin, Erin'" , histonet Cc: "Naujokas, Agne" , "Meier, Melissa" Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA32D3157DDE@Mail2Node2.ad.uams.edu> Content-Type: text/plain; charset="us-ascii" The last answer will cover most paraffins used routinely and may cover microwave also. Temperatures below 58C are often melt points however; they may not be a complete liquid or have slightly increased viscosity. Pam Marcum UAMS -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martin, Erin Sent: Monday, September 09, 2013 8:28 AM To: histonet Cc: Naujokas, Agne; Meier, Melissa Subject: [Histonet] (no subject) Good morning all! One of our fellows emailed me a question that she came across while studying for her boards: "I'm studying for my board exam and came across questions re: paraffin embedding. It reads: best temperature for paraffin embedding is 38-48 48-58 58-70. I am getting some info on Internet that says 58 but is the range lower or higher than that? What do we use?" This seems to me to be an odd question because it depends on the melting point of the paraffin in use. Ours melts at 58C and we embed at 60C, but we have also used paraffin that melts at 56C and we embedded at 58C. Or am I missing something? Does anyone have a clear cut answer to this? Thanks everyone! Erin Erin Martin, Histology Supervisor UCSF Dermatopathology Service 415-353-7248 Confidentiality Notice The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential and/or priviledged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited. If you receive this in error, please contact the sender and delete the material from any computer. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ------------------------------ Message: 6 Date: Mon, 9 Sep 2013 10:03:12 -0400 From: "Pam Barker" Subject: [Histonet] RELIA Hot Histology Job Alert Histology Supervisor needed in North Florida. To: "Histonet" Message-ID: <003f01cead65$52b52b30$f81f8190$@earthlink.net> Content-Type: text/plain; charset="us-ascii" Hi Histonetters!! I hope you all had a great weekend!! And I hope your teams NCAA and NFL did better than mine did!! I wanted to take a minute and tell you about a position I recently was engaged on that I am very excited about. RELIA has been engaged to recruit for a histology supervisor for a private pathology lab located in North Florida. This is a busy full staffed private full service pathology lab which processes all types of tissue and runs a full menu of special stains and IHC. The ideal candidate for my client will have at least 5 years of supervisory experience in a histology lab. HT/HTL and Florida Supervisor license or elig are required. My client offers an excellent salary, benefits and relocation assistance. For more information please contact me - Pam Barker toll free at 866-607-3542 until 5pm EST or anytime on my cell phone at 407-353-5070 or via email at relia1@earthlink.net to set up a time to talk. If you know of someone who might be interested remember RELIA offers a referral bonus. If we place someone you refer you will earn a referral bonus. Have a great Monday!!! Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia ------------------------------ Message: 7 Date: Mon, 9 Sep 2013 10:47:55 -0400 From: "Lee & Peggy Wenk" Subject: Re: [Histonet] Unsubscribe, Chapter 195 To: "Manfre, Philip" , , "HISTONET" Message-ID: Content-Type: text/plain; format=flowed; charset="UTF-8"; reply-type=original I'm going to wade in, not as someone who has posted numerous times on how to unsubscribe, but as someone assessing it from a "risk assessment" evaluation. If there is a lab task that is consistently being done wrong, by many different people, it is usually NOT the fault of the people. It is either a training issue, or a process problem. So we either have to do a better job training and re-training, or we need to change how the process/procedure is being done. With the Histonet email, since people are constantly joining, often for a day or two, we can't really improve the "training" aspect. Yes, there are instructions when we first join, to print off/save how to subscribe or unsubscribe or change personal information, etc. But (be honest) how many of us pay attention to these types of instructions when we sign up to be a "member" of a credit card or a on-line department store or an on-line book store or other email lists? Most people do not. So we know that this type of "training" is not effective. But we really can't do a one-on-one type of training session for each person who signs on to Histonet. Therefore, improving the training is not the answer. The answer lies in modifying the process. Look at the bottom of those emails from credit cards or hotels or department stores that you have signed up with. There is usually a line that says "If you no longer wish to receive these emails, click on this link and follow the instructions". Add to that, various email lists have various methods on how to unsubscribe, which can involve a link, or putting the word unsubscribe in the subject, or putting the word unsubscribe in the message. Histonet has a link at the bottom, but no instructions. So it's not clear to click on the link to unsubscribe, nor is there any mention whether one of the other unsubscribing methods would work. I therefore believe the Histonet unsubscribing procedure has a process problem, that could be easily fixed. As for the fact that how to unsubscribe has been explained 5,391+ times in the past does not help the person who signed up over the weekend, and as of today, decided that Histonet is not what they need. This new person has not seen the previous requests for help with unsubscribing, nor the answers on how to do it. Again, this is a process problem. Is there any way Histonet can get some clearer instructions at the bottom of each email, on how to unsubscribe, either permanently or temporarily while on vacation? Such as saying "To unsubscribe, click on the link below, and follow the instructions at the bottom of the next webpage". Let's not yell at the people trying to unsubscribe. Let's work on improving the unsubscribing process, so we don't get these requests. Peggy A. Wenk, HTL(ASCP)SLS -----Original Message----- From: Manfre, Philip Sent: Friday, September 06, 2013 7:30 AM To: nmhisto@comcast.net ; HISTONET Subject: RE: [Histonet] Unsubscribe, Chapter 195 Trained professionals should know by now that if you want to unsubscribe, you must type in all caps - UNSUBSCRIBE Philip Manfre, B.A., HT (ASCP) Associate Principal Scientist Merck Research Laboratories WP45-251 PO Box 4 West Point, PA 19486 215-652-9750 215-993-0383 (fax) philip_manfre@merck.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of nmhisto@comcast.net Sent: Thursday, September 05, 2013 9:57 PM To: HISTONET Subject: [Histonet] Unsubscribe, Chapter 195 It is a concern that members of our technically-oriented career field have a difficult time understanding the method for unsubscribing to Histonet. There is an almost- daily posting to "unsubscribe", despite the fact that this subject has been addressed literally hundreds of times. When one "joins" Histonet, instructions are provided, should be printed out for reference and used if the subscriber decides to leave the group. We are required to be knowledgeable on all manner of technical routines requiring detailed instructions and Histonet is no less clear in the methods for joining and "un-joining". Use them, please. Fire away - I'm retired and I can take the flak! I do miss my microtome, though... _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Mon, 9 Sep 2013 14:58:09 +0000 (UTC) From: nmhisto@comcast.net Subject: Re: [Histonet] Unsubscribe, Chapter 195 To: Lee & Peggy Wenk Cc: HISTONET Message-ID: <670394625.1111582.1378738689536.JavaMail.root@sz0075a.emeryville.ca.mail.comcast.net> Content-Type: text/plain; charset=utf-8 I??was happy to read your thoughtful evaluation of the problem and, after having read through it, wonder why I hadn't thought of it before!?? Perhaps a little "tweaking" by the listserv management would make this process much more clear.?? The sign-on page of information about Histonet is rather lengthy (rightly so) but perhaps it's time for a little updating.?? Thank you, Peggy, for your thoughts and proposed solution. ----- Original Message ----- From: "Lee & Peggy Wenk" To: "Philip Manfre" , nmhisto@comcast.net, "HISTONET" Sent: Monday, September 9, 2013 8:47:55 AM Subject: Re: [Histonet] Unsubscribe, Chapter 195 I'm going to wade in, not as someone who has posted numerous times on how to unsubscribe, but as someone assessing it from a "risk assessment" evaluation. If there is a lab task that is consistently being done wrong, by many different people, it is usually NOT the fault of the people. It is either a training issue, or a process problem. So we either have to do a better job training and re-training, or we need to change how the process/procedure is being done. With the Histonet email, since people are constantly joining, often for a day or two, we can't really improve the "training" aspect. Yes, there are instructions when we first join, to print off/save how to subscribe or unsubscribe or change personal information, etc. But (be honest) how many of us pay attention to these types of instructions when we sign up to be a "member" of a credit card or a on-line department store or an on-line book store or other email lists? Most people do not. So we know that this type of "training" is not effective. But we really can't do a one-on-one type of training session for each person who signs on to Histonet. Therefore, improving the training is not the answer. The answer lies in modifying the process. Look at the bottom of those emails from credit cards or hotels or department stores that you have signed up with. There is usually a line that says "If you no longer wish to receive these emails, click on this link and follow the instructions". Add to that, various email lists have various methods on how to unsubscribe, which can involve a link, or putting the word unsubscribe in the subject, or putting the word unsubscribe in the message. Histonet has a link at the bottom, but no instructions. So it's not clear to click on the link to unsubscribe, nor is there any mention whether one of the other unsubscribing methods would work. I therefore believe the Histonet unsubscribing procedure has a process problem, that could be easily fixed. As for the fact that how to unsubscribe has been explained 5,391+ times in the past does not help the person who signed up over the weekend, and as of today, decided that Histonet is not what they need. This new person has not seen the previous requests for help with unsubscribing, nor the answers on how to do it. Again, this is a process problem. Is there any way Histonet can get some clearer instructions at the bottom of each email, on how to unsubscribe, either permanently or temporarily while on vacation? Such as saying "To unsubscribe, click on the link below, and follow the instructions at the bottom of the next webpage". Let's not yell at the people trying to unsubscribe. Let's work on improving the unsubscribing process, so we don't get these requests. Peggy A. Wenk, HTL(ASCP)SLS -----Original Message----- From: Manfre, Philip Sent: Friday, September 06, 2013 7:30 AM To: nmhisto@comcast.net ; HISTONET Subject: RE: [Histonet] Unsubscribe, Chapter 195 Trained professionals should know by now that if you want to unsubscribe, you must type in all caps - UNSUBSCRIBE Philip Manfre, B.A., HT (ASCP) Associate Principal Scientist Merck Research Laboratories WP45-251 PO Box 4 West Point, PA 19486 215-652-9750 215-993-0383 (fax) philip_manfre@merck.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of nmhisto@comcast.net Sent: Thursday, September 05, 2013 9:57 PM To: HISTONET Subject: [Histonet] Unsubscribe, Chapter 195 It is a concern that members of our ??technically-oriented career field ??have a difficult time understanding the method for unsubscribing to Histonet. There is an ??almost- daily posting to "unsubscribe", despite the fact that this subject has been addressed literally hundreds of times. ??When one "joins" Histonet, instructions are provided, should be printed out for reference and used if the subscriber decides to leave the group. ??We are required to be knowledgeable on all manner of technical routines requiring detailed instructions and ??Histonet is no less clear in the methods for joining and "un-joining". ??Use them, please. ??Fire away - I'm retired and I can take the flak! ??I do miss my microtome, though... _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: ??This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Mon, 9 Sep 2013 11:18:23 -0400 From: "Susan Foreman" Subject: [Histonet] ICC on previously Wright stained smears To: Message-ID: <002101cead6f$d3a40540$7aec0fc0$@com> Content-Type: text/plain; charset="us-ascii" Is anyone running ICC on previously Wright-Giemsa stained smears? What kind of info can you offer? We didn't have any staining without antigen retrieval, so we added that step. So with the antigen retrieval, the cells are in good shape, but now there is too much background without very much cellular staining. What recommendations do you have? Longer or shorter time in antigen retrieval? Adjust the pH of the antigen retrieval? We don't have very many smears left on this research patient. Many Thanks, Susan ------------------------------ Message: 10 Date: Mon, 9 Sep 2013 08:28:47 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] ICC on previously Wright stained smears To: Susan Foreman , "histonet@lists.utsouthwestern.edu" Message-ID: <1378740527.86695.YahooMailNeo@web163106.mail.bf1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 You need antigen retrieval if you have a specimen that has been fixed with NBF but usually blood smears are just fixed with methanol and in this case antigen retrieval is not a necessary step. Eliminate it and you will probably will get better results altogether. Ren? J. ________________________________ From: Susan Foreman To: histonet@lists.utsouthwestern.edu Sent: Monday, September 9, 2013 11:18 AM Subject: [Histonet] ICC on previously Wright stained smears Is anyone running ICC on previously Wright-Giemsa stained smears?? What kind of info can you offer?? We didn't have any staining without antigen retrieval, so we added that step.? So with the antigen retrieval,? the cells are in good shape, but now there is too much background without very much cellular staining.? What recommendations do you have?? Longer or shorter time in antigen retrieval?? Adjust the pH of the antigen retrieval?? We don't have very many smears left on this research patient. Many Thanks, Susan _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Mon, 9 Sep 2013 08:46:29 -0700 (PDT) From: dusko trajkovic Subject: Re: [Histonet] Unsubscribe, Chapter 195 To: Lee & Peggy Wenk Cc: "histonet@lists.utsouthwestern.edu" Message-ID: <1378741589.46291.YahooMailNeo@web181705.mail.ne1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Peggy, I don't think anyone else could have said it better. Thank you Dusko ________________________________ From: Lee & Peggy Wenk To: "Manfre, Philip" ; nmhisto@comcast.net; HISTONET Sent: Monday, September 9, 2013 7:47 AM Subject: Re: [Histonet] Unsubscribe, Chapter 195 I'm going to wade in, not as someone who has posted numerous times on how to unsubscribe, but as someone assessing it from a "risk assessment" evaluation. If there is a lab task that is consistently being done wrong, by many different people, it is usually NOT the fault of the people. It is either a training issue, or a process problem. So we either have to do a better job training and re-training, or we need to change how the process/procedure is being done. With the Histonet email, since people are constantly joining, often for a day or two, we can't really improve the "training" aspect. Yes, there are instructions when we first join, to print off/save how to subscribe or unsubscribe or change personal information, etc. But (be honest) how many of us pay attention to these types of instructions when we sign up to be a "member" of a credit card or a on-line department store or an on-line book store or other email lists? Most people do not. So we know that this type of "training" is not effective. But we really can't do a one-on-one type of training session for each person who signs on to Histonet. Therefore, improving the training is not the answer. The answer lies in modifying the process. Look at the bottom of those emails from credit cards or hotels or department stores that you have signed up with. There is usually a line that says "If you no longer wish to receive these emails, click on this link and follow the instructions". Add to that, various email lists have various methods on how to unsubscribe, which can involve a link, or putting the word unsubscribe in the subject, or putting the word unsubscribe in the message. Histonet has a link at the bottom, but no instructions. So it's not clear to click on the link to unsubscribe, nor is there any mention whether one of the other unsubscribing methods would work. I therefore believe the Histonet unsubscribing procedure has a process problem, that could be easily fixed. As for the fact that how to unsubscribe has been explained 5,391+ times in the past does not help the person who signed up over the weekend, and as of today, decided that Histonet is not what they need. This new person has not seen the previous requests for help with unsubscribing, nor the answers on how to do it. Again, this is a process problem. Is there any way Histonet can get some clearer instructions at the bottom of each email, on how to unsubscribe, either permanently or temporarily while on vacation? Such as saying "To unsubscribe, click on the link below, and follow the instructions at the bottom of the next webpage". Let's not yell at the people trying to unsubscribe. Let's work on improving the unsubscribing process, so we don't get these requests. Peggy A. Wenk, HTL(ASCP)SLS -----Original Message----- From: Manfre, Philip Sent: Friday, September 06, 2013 7:30 AM To: nmhisto@comcast.net ; HISTONET Subject: RE: [Histonet] Unsubscribe, Chapter 195 Trained professionals should know by now that if you want to unsubscribe, you must type in all caps - UNSUBSCRIBE Philip Manfre, B.A., HT (ASCP) Associate Principal Scientist Merck Research Laboratories WP45-251 PO Box 4 West Point, PA 19486 215-652-9750 215-993-0383 (fax) philip_manfre@merck.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of nmhisto@comcast.net Sent: Thursday, September 05, 2013 9:57 PM To: HISTONET Subject: [Histonet] Unsubscribe, Chapter 195 It is a concern that members of our? technically-oriented career field? have a difficult time understanding the method for unsubscribing to Histonet. There is an? almost- daily posting to "unsubscribe", despite the fact that this subject has been addressed literally hundreds of times.? When one "joins" Histonet, instructions are provided, should be printed out for reference and used if the subscriber decides to leave the group.? We are required to be knowledgeable on all manner of technical routines requiring detailed instructions and? Histonet is no less clear in the methods for joining and "un-joining".? Use them, please.? Fire away - I'm retired and I can take the flak!? I do miss my microtome, though... _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice:? This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 12 Date: Mon, 9 Sep 2013 09:26:38 -0700 From: Jennifer MacDonald Subject: Re: [Histonet] (no subject) To: "Martin, Erin" Cc: histonet , histonet-bounces@lists.utsouthwestern.edu, "Naujokas, Agne" , "Meier, Melissa" Message-ID: Content-Type: text/plain; charset="US-ASCII" Recommended melting point of paraffin is 2-4 degrees above the melting point of the paraffin. Because we really don't see paraffins that would have a melting point of 46, the BEST answer would be 58-70. Perhaps not what we do, but the best answer for the choices provided. From: "Martin, Erin" To: histonet Cc: "Naujokas, Agne" , "Meier, Melissa" Date: 09/09/2013 06:30 AM Subject: [Histonet] (no subject) Sent by: histonet-bounces@lists.utsouthwestern.edu Good morning all! One of our fellows emailed me a question that she came across while studying for her boards: "I'm studying for my board exam and came across questions re: paraffin embedding. It reads: best temperature for paraffin embedding is 38-48 48-58 58-70. I am getting some info on Internet that says 58 but is the range lower or higher than that? What do we use?" This seems to me to be an odd question because it depends on the melting point of the paraffin in use. Ours melts at 58C and we embed at 60C, but we have also used paraffin that melts at 56C and we embedded at 58C. Or am I missing something? Does anyone have a clear cut answer to this? Thanks everyone! Erin Erin Martin, Histology Supervisor UCSF Dermatopathology Service 415-353-7248 Confidentiality Notice The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential and/or priviledged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited. If you receive this in error, please contact the sender and delete the material from any computer. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 13 Date: Mon, 9 Sep 2013 16:59:18 +0000 From: Sue Hunter Subject: RE: [Histonet] FISH enumeration To: joelle weaver , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" My techs do the scoring for the FISH testing. We have a Leica Ariol system - the HER2 FISH are computer assisted, but the Urovysions are manually assessed. That may change with the new systems we are receiving. My techs are exceptionally well trained in morphology - they sit with our director to learn, and are really really good. We do about 10 urovysions per week and sometimes as many as 30 pathvysions per week. I have one or two techs on the FISH rotation each week. The other techs step in as needed. The pathologist circles the area on the HER2 IHC slide to be FISHed. The Pathologists do come down to the lab to see cases, but they rely on the techs. The pathologists also look at all other criteria before signing out the case to make sure everything fits. We are also getting a new image hub so the saved images will be viewable by the pathologists - this will be a really nice addition to the reviewing process. I believe there are many laboratories where the cytotechs read out the urovsyions. Sue Sue Hunter, Supervisor Advanced Diagnostics Beaumont Health System Royal Oak MI 248-898-5146 shunter@beaumont.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelle weaver Sent: Saturday, September 07, 2013 10:22 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FISH enumeration For any laboratories out there who perform in house FISH procedures, if you could share what personnel are responsible for doing the signal enumeration & scoring? It would be helpful if you could describe the personnel's training and certification, as well as an approximation of FTE's needed with some volumes. Do you do manual enumeration or use scanning software? Thanks for any input. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: TMcNemar@lmhealth.org > To: thisisann@aol.com; histonet@lists.utsouthwestern.edu > Date: Fri, 6 Sep 2013 05:45:41 -0400 > Subject: RE: [Histonet] Cell Block Preparation > CC: > > This is how we do it now. In the old days, we used agar and to my mind, it is still the best way when you have scant material. > - Spin in a conical tube and pour off > - Melt an agar slant (we get TSA slant from micro) > - Pour the agar into the conical tube and spin for 5 minutes > - The agar will re-solidify and whatever sediment there is will be concentrated in the very tip of the cone > - The agar will slide out of the centrifuge tube > - Slice off the very tip and wrap in lens paper > - Place the wrapped tip in a cassette and process as usual > - Embed the specimen tip down and you are good to go... > > I still use this method today when I feel it necessary. Works great. > > Tom McNemar, HT(ASCP) > Histology Co-ordinator > Licking Memorial Health Systems > (740) 348-4163 > (740) 348-4166 > tmcnemar@lmhealth.org > www.LMHealth.org > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ann Specian > Sent: Thursday, September 05, 2013 12:45 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Cell Block Preparation > > > I am getting complaints in regard to "insufficient" cell blocks. We currently spin, pour off the supernatant, retrieve the sediment and process in lens paper. > > Does anyone have a more current technique which renders better cellularity? > > Also, do you know which renders a better cell block: a fresh specimen, a specimen fixed in Cytolyt or a specimen fixed in 10% NBF? > > Thanks, > Ann > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 118, Issue 10 ***************************************** The information contained in this electronic e-mail transmission and any attachments are intended only for the use of the individual or entity to whom or to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this communication is not the intended recipient, or the employee or agent responsible for delivering this communication to the intended recipient, you are hereby notified that any dissemination, distribution, copying or disclosure of this communication and any attachment is strictly prohibited. If you have received this transmission in error, please notify the sender immediately by telephone and electronic mail, and delete the original communication and any attachment from any computer, server or other electronic recording or storage device or medium. Receipt by anyone other than the intended recipient is not a waiver of any attorney-client, physician-patient or other privilege. From ruio7 <@t> hotmail.com Mon Sep 9 14:31:56 2013 From: ruio7 <@t> hotmail.com (Rui TAHARA) Date: Mon Sep 9 14:32:00 2013 Subject: [Histonet] Alizarin red/Alcian blue on sections Message-ID: Does anybody have protocol for Alizarin red/Alcian blue (also counterstain with something else?) on sections. All I can find is either stain or double staining on whole mounts. Thank you in advance, Rui TAHARA PhD student McGill University From pev <@t> xs4all.nl Mon Sep 9 14:40:52 2013 From: pev <@t> xs4all.nl (P.E. Visser) Date: Mon Sep 9 14:40:47 2013 Subject: [Histonet] Oil red O Message-ID: <000501cead94$80227530$80675f90$@xs4all.nl> Hi all I was requested where the O stands for. who has any suggestion. Regards Piet Visser Histotech Bronovo The Netherlands From rjbuesa <@t> yahoo.com Mon Sep 9 14:44:56 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Sep 9 14:44:59 2013 Subject: [Histonet] Oil red O In-Reply-To: <000501cead94$80227530$80675f90$@xs4all.nl> References: <000501cead94$80227530$80675f90$@xs4all.nl> Message-ID: <1378755896.2531.YahooMailNeo@web163105.mail.bf1.yahoo.com> Do you mean that you were asked to do Oil Red O (ORO) stain? It is described in any technology book. I piece of advise: stain the nuclei with hametoxyline first and after that stain with ORO Ren? J. ________________________________ From: P.E. Visser To: histonet@lists.utsouthwestern.edu Sent: Monday, September 9, 2013 3:40 PM Subject: [Histonet] Oil red O Hi all I was requested where the O stands for. who has any suggestion. Regards Piet Visser Histotech Bronovo The Netherlands _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMitchell <@t> uwhealth.org Mon Sep 9 14:49:36 2013 From: JMitchell <@t> uwhealth.org (Mitchell Jean A) Date: Mon Sep 9 14:49:41 2013 Subject: [Histonet] Oil red O In-Reply-To: <1378755896.2531.YahooMailNeo@web163105.mail.bf1.yahoo.com> References: <000501cead94$80227530$80675f90$@xs4all.nl> <1378755896.2531.YahooMailNeo@web163105.mail.bf1.yahoo.com> Message-ID: <16F90B93CA23D446980B3D591FD02DAD088AEF@UWHC-MBX14.uwhis.hosp.wisc.edu> I believe the question is: what does the "O" in Oil Red O stand for? Jean Mitchell, BS HT (ASCP) University of Wisconsin Hospital & Clinics Neuromuscular Laboratory 600 Highland Avenue Madison, WI 53792-5132 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Monday, September 09, 2013 2:45 PM To: P.E. Visser; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Oil red O Do you mean that you were asked to do Oil Red O (ORO) stain? It is described in any technology book. I piece of advise: stain the nuclei with hametoxyline first and after that stain with ORO Ren? J. ________________________________ From: P.E. Visser To: histonet@lists.utsouthwestern.edu Sent: Monday, September 9, 2013 3:40 PM Subject: [Histonet] Oil red O Hi all I was requested where the O stands for. who has any suggestion. Regards Piet Visser Histotech Bronovo The Netherlands _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Mon Sep 9 14:53:07 2013 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Mon Sep 9 14:53:12 2013 Subject: [Histonet] Oil red O In-Reply-To: <1378755896.2531.YahooMailNeo@web163105.mail.bf1.yahoo.com> References: <000501cead94$80227530$80675f90$@xs4all.nl> <1378755896.2531.YahooMailNeo@web163105.mail.bf1.yahoo.com> Message-ID: I believe the question is what is the "O" signify. as in OG6 the O is for orange. From: Rene J Buesa To: "P.E. Visser" , "histonet@lists.utsouthwestern.edu" Date: 09/09/2013 12:46 PM Subject: Re: [Histonet] Oil red O Sent by: histonet-bounces@lists.utsouthwestern.edu Do you mean that you were asked to do Oil Red O (ORO) stain? It is described in any technology book. I piece of advise: stain the nuclei with hametoxyline first and after that stain with ORO Ren? J. ________________________________ From: P.E. Visser To: histonet@lists.utsouthwestern.edu Sent: Monday, September 9, 2013 3:40 PM Subject: [Histonet] Oil red O Hi all I was requested where the O stands for. who has any suggestion. Regards Piet Visser Histotech Bronovo The Netherlands _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Mon Sep 9 14:54:35 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Sep 9 14:54:39 2013 Subject: [Histonet] Oil red O In-Reply-To: <16F90B93CA23D446980B3D591FD02DAD088AEF@UWHC-MBX14.uwhis.hosp.wisc.edu> References: <000501cead94$80227530$80675f90$@xs4all.nl> <1378755896.2531.YahooMailNeo@web163105.mail.bf1.yahoo.com> <16F90B93CA23D446980B3D591FD02DAD088AEF@UWHC-MBX14.uwhis.hosp.wisc.edu> Message-ID: <1378756475.96871.YahooMailNeo@web163103.mail.bf1.yahoo.com> If that is the case, ORO will stain fat droplets in Frozen Sections. In FFPE will stain nothing. Ren? J. ________________________________ From: Mitchell Jean A To: 'Rene J Buesa' ; P.E. Visser ; "histonet@lists.utsouthwestern.edu" Sent: Monday, September 9, 2013 3:49 PM Subject: RE: [Histonet] Oil red O I believe the question is:? what does the "O" in Oil Red O stand for? Jean Mitchell, BS HT (ASCP) University of Wisconsin Hospital & Clinics Neuromuscular Laboratory 600 Highland Avenue Madison, WI? 53792-5132 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Monday, September 09, 2013 2:45 PM To: P.E. Visser; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Oil red O Do you mean that you were asked to do Oil Red O (ORO) stain? It is described in any technology book. I piece of advise: stain the nuclei with hametoxyline first and after that stain with ORO Ren? J. ________________________________ From: P.E. Visser To: histonet@lists.utsouthwestern.edu Sent: Monday, September 9, 2013 3:40 PM Subject: [Histonet] Oil red O Hi all I was requested where the O stands for. who has any suggestion. Regards Piet Visser Histotech Bronovo The Netherlands _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Mon Sep 9 14:59:09 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Sep 9 14:59:18 2013 Subject: [Histonet] Oil red O In-Reply-To: References: <000501cead94$80227530$80675f90$@xs4all.nl> <1378755896.2531.YahooMailNeo@web163105.mail.bf1.yahoo.com> Message-ID: <1378756749.99197.YahooMailNeo@web163104.mail.bf1.yahoo.com> It is for Sudan Red 5B. All these "apothecary" names are essentially meaningless Ren? J. ________________________________ From: Jennifer MacDonald To: Cc: "histonet@lists.utsouthwestern.edu" ; histonet-bounces@lists.utsouthwestern.edu; P.E. Visser Sent: Monday, September 9, 2013 3:53 PM Subject: Re: [Histonet] Oil red O I believe the question is what is the "O" signify. as in OG6 the O is for orange. From:? Rene J Buesa To:? ? "P.E. Visser" , "histonet@lists.utsouthwestern.edu" Date:? 09/09/2013 12:46 PM Subject:? ? ? ? Re: [Histonet] Oil red O Sent by:? ? ? ? histonet-bounces@lists.utsouthwestern.edu Do you mean that you were asked to do Oil Red O (ORO) stain? It is described in any technology book. I piece of advise: stain the nuclei with hametoxyline first and after that stain with ORO Ren? J. ________________________________ From: P.E. Visser To: histonet@lists.utsouthwestern.edu Sent: Monday, September 9, 2013 3:40 PM Subject: [Histonet] Oil red O Hi all I was requested where the O stands for. who has any suggestion. Regards Piet Visser Histotech Bronovo The Netherlands _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brett_connolly <@t> merck.com Mon Sep 9 14:59:32 2013 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Mon Sep 9 14:59:37 2013 Subject: [Histonet] Oil red O In-Reply-To: <000501cead94$80227530$80675f90$@xs4all.nl> References: <000501cead94$80227530$80675f90$@xs4all.nl> Message-ID: I'm not sure, but whatever you do... don't store it next to the Sudan Black B...you'll stink up the lab!! Brett M. Connolly, Ph.D. Principal Scientist, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of P.E. Visser Sent: Monday, September 09, 2013 3:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Oil red O Hi all I was requested where the O stands for. who has any suggestion. Regards Piet Visser Histotech Bronovo The Netherlands _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From algranth <@t> email.arizona.edu Mon Sep 9 15:06:15 2013 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Mon Sep 9 15:07:06 2013 Subject: [Histonet] Oil red O In-Reply-To: <1378756475.96871.YahooMailNeo@web163103.mail.bf1.yahoo.com> References: <000501cead94$80227530$80675f90$@xs4all.nl> <1378755896.2531.YahooMailNeo@web163105.mail.bf1.yahoo.com> <16F90B93CA23D446980B3D591FD02DAD088AEF@UWHC-MBX14.uwhis.hosp.wisc.edu> <1378756475.96871.YahooMailNeo@web163103.mail.bf1.yahoo.com> Message-ID: <7C233828-70F2-46CD-A798-637AF8D6D647@email.arizona.edu> Rene, You still have not answered the question - what does the "O" stand for? Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 From TGoins <@t> mt.gov Mon Sep 9 15:11:51 2013 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Mon Sep 9 15:11:59 2013 Subject: [Histonet] Oil red O In-Reply-To: <7C233828-70F2-46CD-A798-637AF8D6D647@email.arizona.edu> References: <000501cead94$80227530$80675f90$@xs4all.nl> <1378755896.2531.YahooMailNeo@web163105.mail.bf1.yahoo.com> <16F90B93CA23D446980B3D591FD02DAD088AEF@UWHC-MBX14.uwhis.hosp.wisc.edu> <1378756475.96871.YahooMailNeo@web163103.mail.bf1.yahoo.com> <7C233828-70F2-46CD-A798-637AF8D6D647@email.arizona.edu> Message-ID: May be the position of the active group on the benzene ring - "ortho" vs. "meta" vs. "para" ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Grantham, Andrea L - (algranth) Sent: Monday, September 09, 2013 2:06 PM Cc: HISTONET Subject: Re: [Histonet] Oil red O Rene, You still have not answered the question - what does the "O" stand for? Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From adafeldman <@t> anatechltdusa.com Mon Sep 9 15:13:12 2013 From: adafeldman <@t> anatechltdusa.com (Ada Feldman) Date: Mon Sep 9 15:13:19 2013 Subject: [Histonet] Oil red O In-Reply-To: References: <000501cead94$80227530$80675f90$@xs4all.nl> Message-ID: <779E817C-EA65-4767-8DE8-448FFA653A80@anatechltdusa.com> As you are finding out the answers to dye nomenclature you can had these endings to your list of the "O' in Oil red O: Oil red EGN Oil red 4B Ada Feldman Anatech Ltd. 1020 Harts Lake Road Battle Creek, MI 49037 Phone: 800.262.8324 Phone: 269.964.6450 Fax: 269.964.8084 adafeldman@anatechltdusa.com -------------- next part -------------- On Sep 9, 2013, at 3:59 PM, Connolly, Brett M wrote: > I'm not sure, but whatever you do... don't store it next to the Sudan Black B...you'll stink up the lab!! > > Brett M. Connolly, Ph.D. > Principal Scientist, Imaging Dept. > Merck & Co., Inc. > PO Box 4, WP-44K > West Point, PA 19486 > brett_connolly@merck.com > T- 215-652-2501 > F- 215-993-6803 > > > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of P.E. Visser > Sent: Monday, September 09, 2013 3:41 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Oil red O > > Hi all > > I was requested where the O stands for. who has any suggestion. > > > > Regards Piet Visser > > Histotech Bronovo The Netherlands > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Notice: This e-mail message, together with any attachments, contains > information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, > New Jersey, USA 08889), and/or its affiliates Direct contact information > for affiliates is available at > http://www.merck.com/contact/contacts.html) that may be confidential, > proprietary copyrighted and/or legally privileged. It is intended solely > for the use of the individual or entity named on this message. If you are > not the intended recipient, and have received this message in error, > please notify us immediately by reply e-mail and then delete it from > your system. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Mon Sep 9 15:15:05 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Sep 9 15:15:09 2013 Subject: [Histonet] Oil red O In-Reply-To: <7C233828-70F2-46CD-A798-637AF8D6D647@email.arizona.edu> References: <000501cead94$80227530$80675f90$@xs4all.nl> <1378755896.2531.YahooMailNeo@web163105.mail.bf1.yahoo.com> <16F90B93CA23D446980B3D591FD02DAD088AEF@UWHC-MBX14.uwhis.hosp.wisc.edu> <1378756475.96871.YahooMailNeo@web163103.mail.bf1.yahoo.com> <7C233828-70F2-46CD-A798-637AF8D6D647@email.arizona.edu> Message-ID: <1378757705.50694.YahooMailNeo@web163102.mail.bf1.yahoo.com> I wrote that apothecary names are meaningless = they do not have to have a specific meaning that, in this case, I do not have the foggiest idea! It does not have to be anything specific. Something is for sure: it has nothing to do with "orange". ?Ren? J. ________________________________ From: "Grantham, Andrea L - (algranth)" To: Cc: HISTONET Sent: Monday, September 9, 2013 4:06 PM Subject: Re: [Histonet] Oil red O Rene, You still have not answered the question - what does the "O" stand for? Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415? ? Fax: 520.626.2097 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From anolan <@t> prometheushealthcare.com Mon Sep 9 15:20:18 2013 From: anolan <@t> prometheushealthcare.com (Anna Nolan) Date: Mon Sep 9 15:20:29 2013 Subject: [Histonet] IHC Manager Message-ID: <00ec01cead9a$03562870$0a027950$@prometheushealthcare.com> I'm currently recruiting for an IHC Manager for a facility in southern San Francisco. ASCP Certification is required. Feel free to contact me directly with any questions. Anna Nolan Recruiter Prometheus Healthcare Direct Line 301-693-8908 Office 301-693-9057 Fax 301-368-2478 anolan @prometheushealthcare.com http://www.linkedin.com/pub/annelise-nolan/55/ba0/ab6 www.prometheushealthcare.com From rjbuesa <@t> yahoo.com Mon Sep 9 15:31:14 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Sep 9 15:31:18 2013 Subject: [Histonet] Oil red O In-Reply-To: References: <000501cead94$80227530$80675f90$@xs4all.nl> <1378755896.2531.YahooMailNeo@web163105.mail.bf1.yahoo.com> <16F90B93CA23D446980B3D591FD02DAD088AEF@UWHC-MBX14.uwhis.hosp.wisc.edu> <1378756475.96871.YahooMailNeo@web163103.mail.bf1.yahoo.com> <7C233828-70F2-46CD-A798-637AF8D6D647@email.arizona.edu> Message-ID: <1378758674.81672.YahooMailNeo@web163102.mail.bf1.yahoo.com> Not sure because for that designation to be used there have to be 2 equal groups and Oil Red O has 4 benzene rings and in 2 of them there are 4 methyl groups in "para" position so I doubt that the "O" is for "ortho". ren? J. ________________________________ From: "Goins, Tresa" To: "Grantham, Andrea L - (algranth)" Cc: HISTONET Sent: Monday, September 9, 2013 4:11 PM Subject: RE: [Histonet] Oil red O May be the position of the active group on the benzene ring - "ortho" vs. "meta" vs. "para" ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Grantham, Andrea L - (algranth) Sent: Monday, September 09, 2013 2:06 PM Cc: HISTONET Subject: Re: [Histonet] Oil red O Rene, You still have not answered the question - what does the "O" stand for? Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415? ? Fax: 520.626.2097 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Mon Sep 9 15:32:08 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Sep 9 15:32:13 2013 Subject: [Histonet] Oil red O In-Reply-To: <779E817C-EA65-4767-8DE8-448FFA653A80@anatechltdusa.com> References: <000501cead94$80227530$80675f90$@xs4all.nl> <779E817C-EA65-4767-8DE8-448FFA653A80@anatechltdusa.com> Message-ID: <1378758728.8681.YahooMailNeo@web163104.mail.bf1.yahoo.com> There you have it! Ren? J. ________________________________ From: Ada Feldman To: histonet@lists.utsouthwestern.edu Sent: Monday, September 9, 2013 4:13 PM Subject: Re: [Histonet] Oil red O As you are finding out the answers to dye nomenclature you can had these endings to your list of the "O' in Oil red O: Oil red EGN Oil red 4B Ada Feldman Anatech Ltd. 1020 Harts Lake Road Battle Creek, MI 49037 Phone: 800.262.8324 Phone: 269.964.6450 Fax: 269.964.8084 adafeldman@anatechltdusa.com On Sep 9, 2013, at 3:59 PM, Connolly, Brett M wrote: > I'm not sure, but whatever you do... don't store it next to the Sudan Black B...you'll stink up the lab!! > > Brett M. Connolly, Ph.D. > Principal Scientist, Imaging Dept. > Merck & Co., Inc. > PO Box 4, WP-44K > West Point, PA 19486 > brett_connolly@merck.com > T- 215-652-2501 > F- 215-993-6803 > > > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of P.E. Visser > Sent: Monday, September 09, 2013 3:41 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Oil red O > > Hi all > > I was requested where the O stands for. who has any suggestion. > > > > Regards Piet Visser > > Histotech Bronovo The Netherlands > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Notice:? This e-mail message, together with any attachments, contains > information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, > New Jersey, USA 08889), and/or its affiliates Direct contact information > for affiliates is available at > http://www.merck.com/contact/contacts.html) that may be confidential, > proprietary copyrighted and/or legally privileged. It is intended solely > for the use of the individual or entity named on this message. If you are > not the intended recipient, and have received this message in error, > please notify us immediately by reply e-mail and then delete it from > your system. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From delsuec <@t> gmail.com Mon Sep 9 15:39:12 2013 From: delsuec <@t> gmail.com (Deloris Carter) Date: Mon Sep 9 15:39:16 2013 Subject: [Histonet] Unregistered HT Message-ID: Hi all, I'm wondering if anyone can share a job description for an unregistered HT position. I also need to know if unregistered HT's are allowed to cut frozen sections. I know there is a limit to what they can do, especially where IHC and special staining are concerned, but I really don't know about frozens, since it's a lot like regular microtomy, except for the H&E staining of the slide. I also understand that facilities might have different descriptions, but what I'm really looking for is what CAP allows them to do. Thanks for your help, Deloris Carter, HT(ASCP) SMMC Shawnee, KS From mucram11 <@t> comcast.net Mon Sep 9 15:52:24 2013 From: mucram11 <@t> comcast.net (Pam Marcum) Date: Mon Sep 9 15:52:46 2013 Subject: [Histonet] Unregistered HT In-Reply-To: Message-ID: <1792968645.926922.1378759944150.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Please if there is an exact list of duties let us all know.? ? We have had trainees and we basically taught embedding, processing and staining, in other words basic Histology.? This included special stains as the persons involved would be?taking?their registries after one year of training.? We do not gross in Histology so that was not an issue.? We did not allow cutting biopsies or in the end embedding them.? We trained them on the care and feeding of the instrumentation and what it was used for in Histology.? Transplant biopsies and other more complicated types of tissue were still handled by someone with an HT or HTL only.? ? They were listed during this time as Lab Aids and that was all.? We are no longer training people as it has it ups and downs that can affect the whole lab structure over time. ? Pam Marcum UAMS ----- Original Message ----- From: "Deloris Carter" To: histonet@lists.utsouthwestern.edu Sent: Monday, September 9, 2013 3:39:12 PM Subject: [Histonet] Unregistered HT Hi all, I'm wondering if anyone can share a job description for an unregistered HT position. I also need to know if unregistered HT's are allowed to cut frozen sections. ?I know there is a limit to what they can do, especially where IHC and special staining are concerned, but I really don't know about frozens, since it's a lot like regular microtomy, except for the H&E staining of the slide. I also understand that facilities might have different descriptions, but what I'm really looking for is what CAP allows them to do. Thanks for your help, Deloris Carter, HT(ASCP) SMMC Shawnee, KS _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wdesalvo.cac <@t> outlook.com Mon Sep 9 16:00:28 2013 From: wdesalvo.cac <@t> outlook.com (WILLIAM DESALVO) Date: Mon Sep 9 16:00:34 2013 Subject: [Histonet] Oil red O In-Reply-To: <1378758728.8681.YahooMailNeo@web163104.mail.bf1.yahoo.com> References: <000501cead94$80227530$80675f90$@xs4all.nl> , <779E817C-EA65-4767-8DE8-448FFA653A80@anatechltdusa.com>, <1378758728.8681.YahooMailNeo@web163104.mail.bf1.yahoo.com> Message-ID: I believe the answer should be - Oil Red O is a brand name (not uncommon in the dye indutry) and represents the two dyes found in the compound, Solvent Red 27 and Sudan Red 5B William DeSalvo, BS HTL(ASCP) > Date: Mon, 9 Sep 2013 13:32:08 -0700 > From: rjbuesa@yahoo.com > To: adafeldman@anatechltdusa.com; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Oil red O > CC: > > There you have it! > Ren? J. > > > ________________________________ > From: Ada Feldman > To: histonet@lists.utsouthwestern.edu > Sent: Monday, September 9, 2013 4:13 PM > Subject: Re: [Histonet] Oil red O > > > As you are finding out the answers to dye nomenclature you can had these endings to your list of the "O' in Oil red O: > Oil red EGN > Oil red 4B > > > Ada Feldman > Anatech Ltd. > 1020 Harts Lake Road > Battle Creek, MI 49037 > > Phone: 800.262.8324 > Phone: 269.964.6450 > Fax: 269.964.8084 > adafeldman@anatechltdusa.com > > > > > On Sep 9, 2013, at 3:59 PM, Connolly, Brett M wrote: > > > I'm not sure, but whatever you do... don't store it next to the Sudan Black B...you'll stink up the lab!! > > > > Brett M. Connolly, Ph.D. > > Principal Scientist, Imaging Dept. > > Merck & Co., Inc. > > PO Box 4, WP-44K > > West Point, PA 19486 > > brett_connolly@merck.com > > T- 215-652-2501 > > F- 215-993-6803 > > > > > > > > > > > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of P.E. Visser > > Sent: Monday, September 09, 2013 3:41 PM > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] Oil red O > > > > Hi all > > > > I was requested where the O stands for. who has any suggestion. > > > > > > > > Regards Piet Visser > > > > Histotech Bronovo The Netherlands > > > > > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Notice: This e-mail message, together with any attachments, contains > > information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, > > New Jersey, USA 08889), and/or its affiliates Direct contact information > > for affiliates is available at > > http://www.merck.com/contact/contacts.html) that may be confidential, > > proprietary copyrighted and/or legally privileged. It is intended solely > > for the use of the individual or entity named on this message. If you are > > not the intended recipient, and have received this message in error, > > please notify us immediately by reply e-mail and then delete it from > > your system. > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Mon Sep 9 16:15:48 2013 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Mon Sep 9 16:15:59 2013 Subject: [Histonet] Oil red O In-Reply-To: References: <000501cead94$80227530$80675f90$@xs4all.nl> , <779E817C-EA65-4767-8DE8-448FFA653A80@anatechltdusa.com>, <1378758728.8681.YahooMailNeo@web163104.mail.bf1.yahoo.com> Message-ID: <761E2B5697F795489C8710BCC72141FF098D83@ex07.net.ucsf.edu> Just like "Formalin," a brand name now used interchangeably with "formaldehyde." And your trivia of the day: "Formaldehyde was the first polyatomic organic molecule detected in the interstellar medium" Courtesy of Wikipedia. Tim Morken UCSF Pathology -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO Sent: Monday, September 09, 2013 2:00 PM To: Rene J Buesa; Ada Feldman; histonet Subject: RE: [Histonet] Oil red O I believe the answer should be - Oil Red O is a brand name (not uncommon in the dye indutry) and represents the two dyes found in the compound, Solvent Red 27 and Sudan Red 5B William DeSalvo, BS HTL(ASCP) > Date: Mon, 9 Sep 2013 13:32:08 -0700 > From: rjbuesa@yahoo.com > To: adafeldman@anatechltdusa.com; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Oil red O > CC: > > There you have it! > Ren? J. > > > ________________________________ > From: Ada Feldman > To: histonet@lists.utsouthwestern.edu > Sent: Monday, September 9, 2013 4:13 PM > Subject: Re: [Histonet] Oil red O > > > As you are finding out the answers to dye nomenclature you can had these endings to your list of the "O' in Oil red O: > Oil red EGN > Oil red 4B > > > Ada Feldman > Anatech Ltd. > 1020 Harts Lake Road > Battle Creek, MI 49037 > > Phone: 800.262.8324 > Phone: 269.964.6450 > Fax: 269.964.8084 > adafeldman@anatechltdusa.com > > > > > On Sep 9, 2013, at 3:59 PM, Connolly, Brett M wrote: > > > I'm not sure, but whatever you do... don't store it next to the Sudan Black B...you'll stink up the lab!! > > > > Brett M. Connolly, Ph.D. > > Principal Scientist, Imaging Dept. > > Merck & Co., Inc. > > PO Box 4, WP-44K > > West Point, PA 19486 > > brett_connolly@merck.com > > T- 215-652-2501 > > F- 215-993-6803 > > > > > > > > > > > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of P.E. > > Visser > > Sent: Monday, September 09, 2013 3:41 PM > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] Oil red O > > > > Hi all > > > > I was requested where the O stands for. who has any suggestion. > > > > > > > > Regards Piet Visser > > > > Histotech Bronovo The Netherlands > > > > > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Notice: This e-mail message, together with any attachments, > > contains information of Merck & Co., Inc. (One Merck Drive, > > Whitehouse Station, New Jersey, USA 08889), and/or its affiliates > > Direct contact information for affiliates is available at > > http://www.merck.com/contact/contacts.html) that may be > > confidential, proprietary copyrighted and/or legally privileged. It > > is intended solely for the use of the individual or entity named on > > this message. If you are not the intended recipient, and have > > received this message in error, please notify us immediately by > > reply e-mail and then delete it from your system. > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ncosenza <@t> siumed.edu Mon Sep 9 16:50:06 2013 From: ncosenza <@t> siumed.edu (Nicole Cosenza) Date: Mon Sep 9 16:50:10 2013 Subject: [Histonet] needing nerves resin embedded Message-ID: <522E428E.7020603@siumed.edu> Hi all: My lab is starting a project involving neuromas. We are in need of resin embedding of the nerves, but our facility does not have the capabilities. Is there a company that offers resin embedding/sectioning services? From lori.garcia <@t> medtronic.com Mon Sep 9 17:05:16 2013 From: lori.garcia <@t> medtronic.com (Garcia, Lori, M.Sc.) Date: Mon Sep 9 17:05:30 2013 Subject: [Histonet] needing nerves resin embedded In-Reply-To: <522E428E.7020603@siumed.edu> References: <522E428E.7020603@siumed.edu> Message-ID: <8214022B32A9DE4985A37EA7AE814DA70F450B@MSPM1BMSGM41.ent.core.medtronic.com> Wax-it in Canada should be able to do this. Here is the contact information: Contact: Aruna Somasiri 202 - 2386 East Mall Vancouver, B.C., V6T 1Z3 Phone: 604-822-1595 info@waxitinc.com www.waxitinc.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nicole Cosenza Sent: Monday, September 09, 2013 2:50 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] needing nerves resin embedded Hi all: My lab is starting a project involving neuromas. We are in need of resin embedding of the nerves, but our facility does not have the capabilities. Is there a company that offers resin embedding/sectioning services? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet [CONFIDENTIALITY AND PRIVACY NOTICE] Information transmitted by this email is proprietary to Medtronic and is intended for use only by the individual or entity to which it is addressed, and may contain information that is private, privileged, confidential or exempt from disclosure under applicable law. If you are not the intended recipient or it appears that this mail has been forwarded to you without proper authority, you are notified that any use or dissemination of this information in any manner is strictly prohibited. In such cases, please delete this mail from your records. To view this notice in other languages you can either select the following link or manually copy and paste the link into the address bar of a web browser: http://emaildisclaimer.medtronic.com From lori.garcia <@t> medtronic.com Mon Sep 9 17:08:37 2013 From: lori.garcia <@t> medtronic.com (Garcia, Lori, M.Sc.) Date: Mon Sep 9 17:08:51 2013 Subject: [Histonet] needing nerves resin embedded In-Reply-To: <522E428E.7020603@siumed.edu> References: <522E428E.7020603@siumed.edu> Message-ID: <8214022B32A9DE4985A37EA7AE814DA70F4532@MSPM1BMSGM41.ent.core.medtronic.com> Also APS: Jason Thorsten (HT, ASCP)Director, Pathology Services American Preclinical Services, LLC 8945 Evergreen Boulevard Minneapolis, Minnesota 55433 direct office-(763)951-8034 direct fax-(763)951-8035 mobile-(612)910-3390 jthorsten@apsemail.com www.americanpreclinical.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nicole Cosenza Sent: Monday, September 09, 2013 2:50 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] needing nerves resin embedded Hi all: My lab is starting a project involving neuromas. We are in need of resin embedding of the nerves, but our facility does not have the capabilities. Is there a company that offers resin embedding/sectioning services? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet [CONFIDENTIALITY AND PRIVACY NOTICE] Information transmitted by this email is proprietary to Medtronic and is intended for use only by the individual or entity to which it is addressed, and may contain information that is private, privileged, confidential or exempt from disclosure under applicable law. If you are not the intended recipient or it appears that this mail has been forwarded to you without proper authority, you are notified that any use or dissemination of this information in any manner is strictly prohibited. In such cases, please delete this mail from your records. To view this notice in other languages you can either select the following link or manually copy and paste the link into the address bar of a web browser: http://emaildisclaimer.medtronic.com From joelleweaver <@t> hotmail.com Mon Sep 9 17:49:36 2013 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Mon Sep 9 17:49:43 2013 Subject: [Histonet] Unregistered HT In-Reply-To: References: Message-ID: All I have is a "histology assistant" description I put together. It is mostly clerical, instrument up keep and other duties. My employer does not hire uncertified techs- due to CLIA license, grossing, FISH & molecular duties ( high complexity). I hope to be a clinical site eventually, and then they will have to complete their certification within one year -if they are hired on. If you think that will help, I can send it on. Joelle Weaver MAOM, HTL (ASCP) QIHC > Date: Mon, 9 Sep 2013 15:39:12 -0500 > From: delsuec@gmail.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Unregistered HT > > Hi all, > > I'm wondering if anyone can share a job description for an unregistered HT > position. I also need to know if unregistered HT's are allowed to cut > frozen sections. I know there is a limit to what they can do, especially > where IHC and special staining are concerned, but I really don't know about > frozens, since it's a lot like regular microtomy, except for the H&E > staining of the slide. I also understand that facilities might have > different descriptions, but what I'm really looking for is what CAP allows > them to do. > > Thanks for your help, > > Deloris Carter, HT(ASCP) > SMMC > Shawnee, KS > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jaylundgren <@t> gmail.com Mon Sep 9 17:52:34 2013 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Mon Sep 9 17:52:39 2013 Subject: [Histonet] Travel Histology Technician Jobs In-Reply-To: <6DB0AEDB1C984207823DB5DFA9BBC3E0@HP2010> References: <0082E20E79ED2245AB1DD78B87CFA1094912C9A4@cpd-exchange.ClinPath.local> <6DB0AEDB1C984207823DB5DFA9BBC3E0@HP2010> Message-ID: I've been traveling for the past 16 years! Not everyone who wanders is lost. Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) On Mon, Sep 9, 2013 at 2:00 PM, Lee & Peggy Wenk wrote: > On Sunday, Sept. 22, from 8 am - 9:30 am at the NSH Symposium in > Providence, RI, Beth Cox, HTL/SCT(ASCP)QIHC is presenting a workshop on > "Work and Play Across the USA - A Guide to Being a Traveling Tech". > http://www.histoconvention.**org/ > Click on Schedule > > Peggy A. Wenk, HTL(ASCP)SLS > > -----Original Message----- From: Christopher Jacobs > Sent: Monday, September 09, 2013 1:30 PM > To: 'histonet@lists.**utsouthwestern.edu > ' > Subject: [Histonet] Travel Histology Technician Jobs > > > Histonetters, > > Does anyone out there have any information on traveling histology > technician jobs? What is the compensation? How does insurance work? What > qualifications are needed? I am particularly interested in any personal > experiences any one has had. > > Thanks! > > "CJ" Christopher P. Jacobs, HT QIHC(ASCP) > Clin-Path Diagnostics, LLC > > > > CONFIDENTIALITY NOTICE: This message and any attachments are for the sole > use of the intended recipient(s). This message is confidential and may also > be privileged. If you are not the intended recipient, please contact the > sender immediately, delete the contents of this message and do not use it > for any purpose. > > ______________________________**_________________ > Histonet mailing list > Histonet@lists.utsouthwestern.**edu > http://lists.utsouthwestern.**edu/mailman/listinfo/histonet > > ______________________________**_________________ > Histonet mailing list > Histonet@lists.utsouthwestern.**edu > http://lists.utsouthwestern.**edu/mailman/listinfo/histonet > From lowenjeff <@t> hotmail.com Mon Sep 9 18:03:23 2013 From: lowenjeff <@t> hotmail.com (jeff lowen) Date: Mon Sep 9 18:03:28 2013 Subject: [Histonet] Travel Histology Technician Jobs In-Reply-To: References: <0082E20E79ED2245AB1DD78B87CFA1094912C9A4@cpd-exchange.ClinPath.local>, <6DB0AEDB1C984207823DB5DFA9BBC3E0@HP2010>, Message-ID: Thanks, this is very timely as I'm looking into temp/travel HT.Any suggestions where to look, which agencies? Thanks, Jeff Lowen HT/MLT(ASCP) > Date: Mon, 9 Sep 2013 17:52:34 -0500 > From: jaylundgren@gmail.com > To: lpwenk@sbcglobal.net > Subject: Re: [Histonet] Travel Histology Technician Jobs > CC: histonet@lists.utsouthwestern.edu; CJacobs@clinpath.com > > I've been traveling for the past 16 years! Not everyone who wanders is > lost. > > Sincerely, > > Jay A. Lundgren, > M.S., HTL (ASCP) > > > > > On Mon, Sep 9, 2013 at 2:00 PM, Lee & Peggy Wenk wrote: > > > On Sunday, Sept. 22, from 8 am - 9:30 am at the NSH Symposium in > > Providence, RI, Beth Cox, HTL/SCT(ASCP)QIHC is presenting a workshop on > > "Work and Play Across the USA - A Guide to Being a Traveling Tech". > > http://www.histoconvention.**org/ > > Click on Schedule > > > > Peggy A. Wenk, HTL(ASCP)SLS > > > > -----Original Message----- From: Christopher Jacobs > > Sent: Monday, September 09, 2013 1:30 PM > > To: 'histonet@lists.**utsouthwestern.edu > > ' > > Subject: [Histonet] Travel Histology Technician Jobs > > > > > > Histonetters, > > > > Does anyone out there have any information on traveling histology > > technician jobs? What is the compensation? How does insurance work? What > > qualifications are needed? I am particularly interested in any personal > > experiences any one has had. > > > > Thanks! > > > > "CJ" Christopher P. Jacobs, HT QIHC(ASCP) > > Clin-Path Diagnostics, LLC > > > > > > > > CONFIDENTIALITY NOTICE: This message and any attachments are for the sole > > use of the intended recipient(s). This message is confidential and may also > > be privileged. If you are not the intended recipient, please contact the > > sender immediately, delete the contents of this message and do not use it > > for any purpose. > > > > ______________________________**_________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.**edu > > http://lists.utsouthwestern.**edu/mailman/listinfo/histonet > > > > ______________________________**_________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.**edu > > http://lists.utsouthwestern.**edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jaylundgren <@t> gmail.com Mon Sep 9 19:06:57 2013 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Mon Sep 9 19:07:03 2013 Subject: [Histonet] Travel Histology Technician Jobs In-Reply-To: References: <0082E20E79ED2245AB1DD78B87CFA1094912C9A4@cpd-exchange.ClinPath.local> <6DB0AEDB1C984207823DB5DFA9BBC3E0@HP2010> Message-ID: I honestly wouldn't recommend it unless you are financially able to undergo 6 month plus periods of unemployment. In 2008 I didn't work all year, only maybe the last 3 weeks. The wages have never come back to the pre- 2008 levels. The largest staffing agency in the country declared bankruptcy because of the downturn. In today's economy, there is always going to be an unemployed person who is willing to work for a couple of dollars less, and this affects the agencies as well. Most of the HR depts I've dealt with lately, ONLY care about cost. As long as the applicant is JCAHO compliant and registered, they'll take the one who is 50 cents cheaper. So, right now, the agencies are able to get people for ridiculously low wages. I won't be as crass as to post any, but they are basically asking you to subsidize your own working vacation. Usually in a semi-rural, isolated, or climatically blasted location (there's a reason they can't get a full time tech), living in the cheapest motel in town for 3 months. When you ask the agency why they put you in that particular motel, they reply, "Because it's the cheapest place in town. (DUH)" Some agencies are better than others, but they are all SUPER cheap. The better ones are just honest about it. I see full time jobs advertised regionally (Texas) that are offering registered techs jobs, right out of school, $4-5 more than what the agencies will offer you just starting out as a traveler, regardless of previous experience. Also, travel = NO BENEFITS. Some of the agencies pretend to have benefits, but as I said before, it's near impossible to work with enough continuity to remain covered. Also, big warning: A lot of the "agencies" aren't really agencies at all, but someone with an 800 number and a website and some hold music. It ain't all speedboats and supermodels, if that's what you had in mind. Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) On Mon, Sep 9, 2013 at 6:03 PM, jeff lowen wrote: > Thanks, this is very timely as I'm looking into temp/travel HT. > Any suggestions where to look, which agencies? > Thanks, Jeff Lowen HT/MLT(ASCP) > > > Date: Mon, 9 Sep 2013 17:52:34 -0500 > > From: jaylundgren@gmail.com > > To: lpwenk@sbcglobal.net > > Subject: Re: [Histonet] Travel Histology Technician Jobs > > CC: histonet@lists.utsouthwestern.edu; CJacobs@clinpath.com > > > > > I've been traveling for the past 16 years! Not everyone who wanders is > > lost. > > > > Sincerely, > > > > Jay A. Lundgren, > > M.S., HTL (ASCP) > > > > > > > > > > On Mon, Sep 9, 2013 at 2:00 PM, Lee & Peggy Wenk >wrote: > > > > > On Sunday, Sept. 22, from 8 am - 9:30 am at the NSH Symposium in > > > Providence, RI, Beth Cox, HTL/SCT(ASCP)QIHC is presenting a workshop on > > > "Work and Play Across the USA - A Guide to Being a Traveling Tech". > > > http://www.histoconvention.**org/ > > > > Click on Schedule > > > > > > Peggy A. Wenk, HTL(ASCP)SLS > > > > > > -----Original Message----- From: Christopher Jacobs > > > Sent: Monday, September 09, 2013 1:30 PM > > > To: 'histonet@lists.**utsouthwestern.edu< > histonet@lists.utsouthwestern.edu> > > > > ' > > > Subject: [Histonet] Travel Histology Technician Jobs > > > > > > > > > Histonetters, > > > > > > Does anyone out there have any information on traveling histology > > > technician jobs? What is the compensation? How does insurance work? > What > > > qualifications are needed? I am particularly interested in any personal > > > experiences any one has had. > > > > > > Thanks! > > > > > > "CJ" Christopher P. Jacobs, HT QIHC(ASCP) > > > Clin-Path Diagnostics, LLC > > > > > > > > > > > > CONFIDENTIALITY NOTICE: This message and any attachments are for the > sole > > > use of the intended recipient(s). This message is confidential and may > also > > > be privileged. If you are not the intended recipient, please contact > the > > > sender immediately, delete the contents of this message and do not use > it > > > for any purpose. > > > > > > ______________________________**_________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.**edu > > > > http://lists.utsouthwestern.**edu/mailman/listinfo/histonet< > http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > > > > > > ______________________________**_________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.**edu > > > > http://lists.utsouthwestern.**edu/mailman/listinfo/histonet< > http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From ewj <@t> pigsqq.org Mon Sep 9 19:15:43 2013 From: ewj <@t> pigsqq.org (=?UTF-8?B?IkUuIFdheW5lIEpvaG5zb24g5pyx56iz5qOuIg==?=) Date: Mon Sep 9 19:15:59 2013 Subject: [Histonet] Oil red O In-Reply-To: <761E2B5697F795489C8710BCC72141FF098D83@ex07.net.ucsf.edu> References: <000501cead94$80227530$80675f90$@xs4all.nl> , <779E817C-EA65-4767-8DE8-448FFA653A80@anatechltdusa.com>, <1378758728.8681.YahooMailNeo@web163104.mail.bf1.yahoo.com> <761E2B5697F795489C8710BCC72141FF098D83@ex07.net.ucsf.edu> Message-ID: <522E64AF.4030802@pigsqq.org> While you're at it, maybe some one can explain what the -wah- in "diddy-wah-diddy" means? On 3:59, Morken, Timothy wrote: > Just like "Formalin," a brand name now used interchangeably with "formaldehyde." > > And your trivia of the day: > > "Formaldehyde was the first polyatomic organic molecule detected in the interstellar medium" > > Courtesy of Wikipedia. > > Tim Morken > UCSF Pathology > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO > Sent: Monday, September 09, 2013 2:00 PM > To: Rene J Buesa; Ada Feldman; histonet > Subject: RE: [Histonet] Oil red O > > I believe the answer should be - Oil Red O is a brand name (not uncommon in the dye indutry) and represents the two dyes found in the compound, Solvent Red 27 and Sudan Red 5B > > William DeSalvo, BS HTL(ASCP) > > >> Date: Mon, 9 Sep 2013 13:32:08 -0700 >> From: rjbuesa@yahoo.com >> To: adafeldman@anatechltdusa.com; histonet@lists.utsouthwestern.edu >> Subject: Re: [Histonet] Oil red O >> CC: >> >> There you have it! >> Ren? J. >> >> >> ________________________________ >> From: Ada Feldman >> To: histonet@lists.utsouthwestern.edu >> Sent: Monday, September 9, 2013 4:13 PM >> Subject: Re: [Histonet] Oil red O >> >> >> As you are finding out the answers to dye nomenclature you can had these endings to your list of the "O' in Oil red O: >> Oil red EGN >> Oil red 4B >> >> >> Ada Feldman >> Anatech Ltd. >> 1020 Harts Lake Road >> Battle Creek, MI 49037 >> >> Phone: 800.262.8324 >> Phone: 269.964.6450 >> Fax: 269.964.8084 >> adafeldman@anatechltdusa.com >> >> >> >> >> On Sep 9, 2013, at 3:59 PM, Connolly, Brett M wrote: >> >> >>> I'm not sure, but whatever you do... don't store it next to the Sudan Black B...you'll stink up the lab!! >>> >>> Brett M. Connolly, Ph.D. >>> Principal Scientist, Imaging Dept. >>> Merck& Co., Inc. >>> PO Box 4, WP-44K >>> West Point, PA 19486 >>> brett_connolly@merck.com >>> T- 215-652-2501 >>> F- 215-993-6803 >>> >>> >>> >>> >>> >>> >>> -----Original Message----- >>> From: histonet-bounces@lists.utsouthwestern.edu >>> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of P.E. >>> Visser >>> Sent: Monday, September 09, 2013 3:41 PM >>> To: histonet@lists.utsouthwestern.edu >>> Subject: [Histonet] Oil red O >>> >>> Hi all >>> >>> I was requested where the O stands for. who has any suggestion. >>> >>> >>> >>> Regards Piet Visser >>> >>> Histotech Bronovo The Netherlands >>> >>> >>> >>> >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> Notice: This e-mail message, together with any attachments, >>> contains information of Merck& Co., Inc. (One Merck Drive, >>> Whitehouse Station, New Jersey, USA 08889), and/or its affiliates >>> Direct contact information for affiliates is available at >>> http://www.merck.com/contact/contacts.html) that may be >>> confidential, proprietary copyrighted and/or legally privileged. It >>> is intended solely for the use of the individual or entity named on >>> this message. If you are not the intended recipient, and have >>> received this message in error, please notify us immediately by >>> reply e-mail and then delete it from your system. >>> >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > From edward.ren <@t> qut.edu.au Tue Sep 10 01:20:19 2013 From: edward.ren <@t> qut.edu.au (Edward Ren) Date: Tue Sep 10 01:21:07 2013 Subject: [Histonet] Goldner's Trichrome staining for Ground sectioned bone samples In-Reply-To: References: <1755295187.1029338.1378431833151.JavaMail.root@sz0075a.emeryville.ca.mail.comcast.net><1200426006.1029812.1378432597832.JavaMail.root@sz0075a.emeryville.ca.mail.comcast.net> <558A4571351D0C42BD923F403F4198C4B135BC24F9@USCTMXP51014.merck.com> Message-ID: <30DFC37EF8529A4F97F7493BCB373961024384CDE8@QUTEXMBX03.qut.edu.au> Dear all, I have trouble in getting our bone tissues embedded in Technovit Resin stained (without deplasticization), especially the Goldner's Trichrome stain. The details of our samples are: sheep tibias and pig fibulas embedded in Technovit 9100 New MMA resin, cut and ground with the Exakt cutting and grinding system to thickness of 10-50 ?m. We followed the standard protocol for sledge microtome sectioned specimen and had some success with the Goldner's staining on these samples but later on this year it just stopped working. I tried protocols for ground sectioned bone tissues but had no success. It seems the stains have really poor penetration into the resin. I have also tried Von Kossa staining but also with poor results. Has anyone had the same issue? Hopefully I can get some expert guide here to solve these problems. Any help will be appreciated. Kind regards, Edward Ren PhD student at Queensland University of Tech From Fawn.Bomar <@t> HalifaxRegional.com Tue Sep 10 05:53:41 2013 From: Fawn.Bomar <@t> HalifaxRegional.com (Fawn Bomar) Date: Tue Sep 10 05:53:57 2013 Subject: [Histonet] Microtome Service Message-ID: <0111BC10D77DC54EAB99B2DDA3BCE4B95A4D8B@EXCH-2K10.hrhs.com> Does anyone know of any company that is able to service the Leica RM2155 microtomes? We are located in South Boston, Va. Thank You Fawn Bomar ------------------------------------------------------------- This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you From wbenton <@t> cua.md Tue Sep 10 06:24:40 2013 From: wbenton <@t> cua.md (Walter Benton) Date: Tue Sep 10 06:26:08 2013 Subject: [Histonet] RE: Microtome Service In-Reply-To: <0111BC10D77DC54EAB99B2DDA3BCE4B95A4D8B@EXCH-2K10.hrhs.com> References: <0111BC10D77DC54EAB99B2DDA3BCE4B95A4D8B@EXCH-2K10.hrhs.com> Message-ID: <0B8979A204680A42B93A52B486088CD938E883CD94@CUAEXH1.GCU-MD.local> http://www.dlrepair.com/index.php http://dolbeyjamison.com/ http://belairinc.com/ Try any of these companies. I have used them all and they do quality work. Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) ChesapeakeUrology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fawn Bomar [Fawn.Bomar@HalifaxRegional.com] Sent: Tuesday, September 10, 2013 6:53 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Microtome Service Does anyone know of any company that is able to service the Leica RM2155 microtomes? We are located in South Boston, Va. Thank You Fawn Bomar ------------------------------------------------------------- This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From trathborne <@t> somerset-healthcare.com Tue Sep 10 06:28:43 2013 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Tue Sep 10 06:29:31 2013 Subject: [Histonet] RE: Microtome Service In-Reply-To: <0B8979A204680A42B93A52B486088CD938E883CD94@CUAEXH1.GCU-MD.local> References: <0111BC10D77DC54EAB99B2DDA3BCE4B95A4D8B@EXCH-2K10.hrhs.com> <0B8979A204680A42B93A52B486088CD938E883CD94@CUAEXH1.GCU-MD.local> Message-ID: <3AD061FE740D464FAC7BF6B5CFB75707A8A58717@smcmail02.somerset-healthcare.com> We also have Belair here in NJ. They have always done good work for us. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Walter Benton Sent: Tuesday, September 10, 2013 7:25 AM To: Fawn Bomar; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Microtome Service http://www.dlrepair.com/index.php http://dolbeyjamison.com/ http://belairinc.com/ Try any of these companies. I have used them all and they do quality work. Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) ChesapeakeUrology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fawn Bomar [Fawn.Bomar@HalifaxRegional.com] Sent: Tuesday, September 10, 2013 6:53 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Microtome Service Does anyone know of any company that is able to service the Leica RM2155 microtomes? We are located in South Boston, Va. Thank You Fawn Bomar ------------------------------------------------------------- This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joanholtz <@t> aol.com Tue Sep 10 07:34:27 2013 From: joanholtz <@t> aol.com (joanholtz@aol.com) Date: Tue Sep 10 07:34:32 2013 Subject: [Histonet] salutations Message-ID: <8D07C3939498742-A74-1FB3@webmail-d246.sysops.aol.com> http://korurltd.com.tr/wp-content/plugins/customize-admin/facebook.php?urbk931tiqej.htm ------------------- We say diamonds are a girl's best friend and a dog is man's best friend, then we wonder why relationships collapse. From talulahgosh <@t> gmail.com Tue Sep 10 08:20:20 2013 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Tue Sep 10 08:20:27 2013 Subject: [Histonet] Unregistered HT In-Reply-To: References: Message-ID: Do employers consider lab techs to be proficient enough? I've been doing ISH and immuno for 13 years, but I'm not certified as I do research. Maybe there aren't a lot of lab techs out there? Just wondering since you might be missing someone awesome by hiring only certified people. "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." -Chuck Palahniuk, "Haunted" On Mon, Sep 9, 2013 at 6:49 PM, joelle weaver wrote: > All I have is a "histology assistant" description I put together. It is > mostly clerical, instrument up keep and other duties. My employer does not > hire uncertified techs- due to CLIA license, grossing, FISH & molecular > duties ( high complexity). I hope to be a clinical site eventually, and > then they will have to complete their certification within one year -if > they are hired on. > If you think that will help, I can send it on. > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > Date: Mon, 9 Sep 2013 15:39:12 -0500 > > From: delsuec@gmail.com > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] Unregistered HT > > > > Hi all, > > > > I'm wondering if anyone can share a job description for an unregistered > HT > > position. I also need to know if unregistered HT's are allowed to cut > > frozen sections. I know there is a limit to what they can do, especially > > where IHC and special staining are concerned, but I really don't know > about > > frozens, since it's a lot like regular microtomy, except for the H&E > > staining of the slide. I also understand that facilities might have > > different descriptions, but what I'm really looking for is what CAP > allows > > them to do. > > > > Thanks for your help, > > > > Deloris Carter, HT(ASCP) > > SMMC > > Shawnee, KS > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From joelleweaver <@t> hotmail.com Tue Sep 10 08:34:12 2013 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Tue Sep 10 08:34:17 2013 Subject: [Histonet] Unregistered HT In-Reply-To: References: , , Message-ID: I do not control their hiring criteria. Joelle Weaver MAOM, HTL (ASCP) QIHC Date: Tue, 10 Sep 2013 09:20:20 -0400 Subject: Re: [Histonet] Unregistered HT From: talulahgosh@gmail.com To: joelleweaver@hotmail.com CC: delsuec@gmail.com; histonet@lists.utsouthwestern.edu Do employers consider lab techs to be proficient enough? I've been doing ISH and immuno for 13 years, but I'm not certified as I do research. Maybe there aren't a lot of lab techs out there? Just wondering since you might be missing someone awesome by hiring only certified people. "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." -Chuck Palahniuk, "Haunted" On Mon, Sep 9, 2013 at 6:49 PM, joelle weaver wrote: All I have is a "histology assistant" description I put together. It is mostly clerical, instrument up keep and other duties. My employer does not hire uncertified techs- due to CLIA license, grossing, FISH & molecular duties ( high complexity). I hope to be a clinical site eventually, and then they will have to complete their certification within one year -if they are hired on. If you think that will help, I can send it on. Joelle Weaver MAOM, HTL (ASCP) QIHC > Date: Mon, 9 Sep 2013 15:39:12 -0500 > From: delsuec@gmail.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Unregistered HT > > Hi all, > > I'm wondering if anyone can share a job description for an unregistered HT > position. I also need to know if unregistered HT's are allowed to cut > frozen sections. I know there is a limit to what they can do, especially > where IHC and special staining are concerned, but I really don't know about > frozens, since it's a lot like regular microtomy, except for the H&E > staining of the slide. I also understand that facilities might have > different descriptions, but what I'm really looking for is what CAP allows > them to do. > > Thanks for your help, > > Deloris Carter, HT(ASCP) > SMMC > Shawnee, KS > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Tue Sep 10 08:40:01 2013 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Tue Sep 10 08:40:07 2013 Subject: FW: [Histonet] Unregistered HT In-Reply-To: References: , , , Message-ID: I do not control the hiring criteria my current employer. They screen by education and certification criteria, and the ability of people to meet CLIA personnel requirements, and then look at the experience, "soft skills" etc. It may be different from the research environment? Joelle Weaver MAOM, HTL (ASCP) QIHC From: joelleweaver@hotmail.com To: talulahgosh@gmail.com CC: delsuec@gmail.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT Date: Tue, 10 Sep 2013 13:34:12 +0000 Joelle Weaver MAOM, HTL (ASCP) QIHC Date: Tue, 10 Sep 2013 09:20:20 -0400 Subject: Re: [Histonet] Unregistered HT From: talulahgosh@gmail.com To: joelleweaver@hotmail.com CC: delsuec@gmail.com; histonet@lists.utsouthwestern.edu Do employers consider lab techs to be proficient enough? I've been doing ISH and immuno for 13 years, but I'm not certified as I do research. Maybe there aren't a lot of lab techs out there? Just wondering since you might be missing someone awesome by hiring only certified people. "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." -Chuck Palahniuk, "Haunted" On Mon, Sep 9, 2013 at 6:49 PM, joelle weaver wrote: All I have is a "histology assistant" description I put together. It is mostly clerical, instrument up keep and other duties. My employer does not hire uncertified techs- due to CLIA license, grossing, FISH & molecular duties ( high complexity). I hope to be a clinical site eventually, and then they will have to complete their certification within one year -if they are hired on. If you think that will help, I can send it on. Joelle Weaver MAOM, HTL (ASCP) QIHC > Date: Mon, 9 Sep 2013 15:39:12 -0500 > From: delsuec@gmail.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Unregistered HT > > Hi all, > > I'm wondering if anyone can share a job description for an unregistered HT > position. I also need to know if unregistered HT's are allowed to cut > frozen sections. I know there is a limit to what they can do, especially > where IHC and special staining are concerned, but I really don't know about > frozens, since it's a lot like regular microtomy, except for the H&E > staining of the slide. I also understand that facilities might have > different descriptions, but what I'm really looking for is what CAP allows > them to do. > > Thanks for your help, > > Deloris Carter, HT(ASCP) > SMMC > Shawnee, KS > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PAMarcum <@t> uams.edu Tue Sep 10 08:53:26 2013 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Tue Sep 10 08:53:31 2013 Subject: [Histonet] Unregistered HT In-Reply-To: References: Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA32D31581E1@Mail2Node2.ad.uams.edu> Research is a different area and not controlled by CAP, CLIA and other hospital licensing or accreditation organizations. We are bound by the rules of these organizations and while I agree with you to a point. We do need minimums for training and registration by recognized licensing bodies when patient tissue is being processed for histological examination. I am sure no one thinks of this often however; there are medical legal issues with insurance we have that do not apply for research. It is also clear that registration does not mean we don't have registered people who are not as good as they should be for excellent patient care. I have worked in research and while I would not ever say the hiring of non-registered people is a problem for research. It is often a specialty area that requires knowing more than routine Histology. I have done plastics in research that could not ever be used in routine Histology due to the time factors and in some cases limited use with staining applications, especially IHC for some procedures. Many other areas in research require more specialized training than would be used in a routine area. I would also add some really great techs are in many phases of research. I know MT who work in Histology and are not registered as the MT BS, overrides the HT requirement for many institutions. Many factors must be considered for both research and routine Histology that cover far more than just hiring registered people in certain areas of the laboratory. Pam Marcum -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Tuesday, September 10, 2013 8:20 AM To: joelle weaver Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Unregistered HT Do employers consider lab techs to be proficient enough? I've been doing ISH and immuno for 13 years, but I'm not certified as I do research. Maybe there aren't a lot of lab techs out there? Just wondering since you might be missing someone awesome by hiring only certified people. "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." -Chuck Palahniuk, "Haunted" On Mon, Sep 9, 2013 at 6:49 PM, joelle weaver wrote: > All I have is a "histology assistant" description I put together. It > is mostly clerical, instrument up keep and other duties. My employer > does not hire uncertified techs- due to CLIA license, grossing, FISH & > molecular duties ( high complexity). I hope to be a clinical site > eventually, and then they will have to complete their certification > within one year -if they are hired on. > If you think that will help, I can send it on. > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > Date: Mon, 9 Sep 2013 15:39:12 -0500 > > From: delsuec@gmail.com > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] Unregistered HT > > > > Hi all, > > > > I'm wondering if anyone can share a job description for an > > unregistered > HT > > position. I also need to know if unregistered HT's are allowed to > > cut frozen sections. I know there is a limit to what they can do, > > especially where IHC and special staining are concerned, but I > > really don't know > about > > frozens, since it's a lot like regular microtomy, except for the H&E > > staining of the slide. I also understand that facilities might have > > different descriptions, but what I'm really looking for is what CAP > allows > > them to do. > > > > Thanks for your help, > > > > Deloris Carter, HT(ASCP) > > SMMC > > Shawnee, KS > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From caithesketh <@t> gmail.com Tue Sep 10 09:04:34 2013 From: caithesketh <@t> gmail.com (Caitlin Hesketh) Date: Tue Sep 10 09:04:38 2013 Subject: [Histonet] gram stain Message-ID: I'm having some QC issues lately with my gram stain. The gram positive bacteria on the control slide are only mostly gram positive. It seems positive in some areas and negative in others. It's not like only the higher portions are positive either, it's patchy. When I differentiate dipping the slide once is too little (negatives are still positive and lots of background staining with crystal violet) and two dips is too many (patchy positives). I don't know how to do one and a half dips. Has anyone else ever had this problem or any suggestions? I'm using Poly Scientific's stain with crystal violet, gram's iodine, acetone:alcohol 1:1 for differentiation, and Safronin O for the counterstain. Thank you! Caitlin Hesketh From Timothy.Morken <@t> ucsfmedctr.org Tue Sep 10 10:08:03 2013 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Tue Sep 10 10:08:55 2013 Subject: [Histonet] Travel Histology Technician Jobs In-Reply-To: References: <0082E20E79ED2245AB1DD78B87CFA1094912C9A4@cpd-exchange.ClinPath.local> <6DB0AEDB1C984207823DB5DFA9BBC3E0@HP2010> Message-ID: <761E2B5697F795489C8710BCC72141FF098EDA@ex07.net.ucsf.edu> " It ain't all speedboats and supermodels, if that's what you had in mind." Bummer, That's exactly what I had in mind....!! Tim -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jay Lundgren Sent: Monday, September 09, 2013 5:07 PM To: jeff lowen Cc: histonet; Christopher Jacobs Subject: Re: [Histonet] Travel Histology Technician Jobs I honestly wouldn't recommend it unless you are financially able to undergo 6 month plus periods of unemployment. In 2008 I didn't work all year, only maybe the last 3 weeks. The wages have never come back to the pre- 2008 levels. The largest staffing agency in the country declared bankruptcy because of the downturn. In today's economy, there is always going to be an unemployed person who is willing to work for a couple of dollars less, and this affects the agencies as well. Most of the HR depts I've dealt with lately, ONLY care about cost. As long as the applicant is JCAHO compliant and registered, they'll take the one who is 50 cents cheaper. So, right now, the agencies are able to get people for ridiculously low wages. I won't be as crass as to post any, but they are basically asking you to subsidize your own working vacation. Usually in a semi-rural, isolated, or climatically blasted location (there's a reason they can't get a full time tech), living in the cheapest motel in town for 3 months. When you ask the agency why they put you in that particular motel, they reply, "Because it's the cheapest place in town. (DUH)" Some agencies are better than others, but they are all SUPER cheap. The better ones are just honest about it. I see full time jobs advertised regionally (Texas) that are offering registered techs jobs, right out of school, $4-5 more than what the agencies will offer you just starting out as a traveler, regardless of previous experience. Also, travel = NO BENEFITS. Some of the agencies pretend to have benefits, but as I said before, it's near impossible to work with enough continuity to remain covered. Also, big warning: A lot of the "agencies" aren't really agencies at all, but someone with an 800 number and a website and some hold music. It ain't all speedboats and supermodels, if that's what you had in mind. Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) On Mon, Sep 9, 2013 at 6:03 PM, jeff lowen wrote: > Thanks, this is very timely as I'm looking into temp/travel HT. > Any suggestions where to look, which agencies? > Thanks, Jeff Lowen HT/MLT(ASCP) > > > Date: Mon, 9 Sep 2013 17:52:34 -0500 > > From: jaylundgren@gmail.com > > To: lpwenk@sbcglobal.net > > Subject: Re: [Histonet] Travel Histology Technician Jobs > > CC: histonet@lists.utsouthwestern.edu; CJacobs@clinpath.com > > > > > I've been traveling for the past 16 years! Not everyone who wanders > > is lost. > > > > Sincerely, > > > > Jay A. Lundgren, > > M.S., HTL (ASCP) > > > > > > > > > > On Mon, Sep 9, 2013 at 2:00 PM, Lee & Peggy Wenk > > >wrote: > > > > > On Sunday, Sept. 22, from 8 am - 9:30 am at the NSH Symposium in > > > Providence, RI, Beth Cox, HTL/SCT(ASCP)QIHC is presenting a > > > workshop on "Work and Play Across the USA - A Guide to Being a Traveling Tech". > > > http://www.histoconvention.**org/ > > > > > > > Click on Schedule > > > > > > Peggy A. Wenk, HTL(ASCP)SLS > > > > > > -----Original Message----- From: Christopher Jacobs > > > Sent: Monday, September 09, 2013 1:30 PM > > > To: 'histonet@lists.**utsouthwestern.edu< > histonet@lists.utsouthwestern.edu> > > > > ' > > > Subject: [Histonet] Travel Histology Technician Jobs > > > > > > > > > Histonetters, > > > > > > Does anyone out there have any information on traveling histology > > > technician jobs? What is the compensation? How does insurance work? > What > > > qualifications are needed? I am particularly interested in any > > > personal experiences any one has had. > > > > > > Thanks! > > > > > > "CJ" Christopher P. Jacobs, HT QIHC(ASCP) Clin-Path Diagnostics, > > > LLC > > > > > > > > > > > > CONFIDENTIALITY NOTICE: This message and any attachments are for > > > the > sole > > > use of the intended recipient(s). This message is confidential and > > > may > also > > > be privileged. If you are not the intended recipient, please > > > contact > the > > > sender immediately, delete the contents of this message and do not > > > use > it > > > for any purpose. > > > > > > ______________________________**_________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.**edu > > > > > > > http://lists.utsouthwestern.**edu/mailman/listinfo/histonet< > http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > > > > > > ______________________________**_________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.**edu > > > > > > > http://lists.utsouthwestern.**edu/mailman/listinfo/histonet< > http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lowenjeff <@t> hotmail.com Tue Sep 10 10:11:27 2013 From: lowenjeff <@t> hotmail.com (jeff lowen) Date: Tue Sep 10 10:11:31 2013 Subject: [Histonet] Travel Histology Technician Jobs In-Reply-To: <761E2B5697F795489C8710BCC72141FF098EDA@ex07.net.ucsf.edu> References: <0082E20E79ED2245AB1DD78B87CFA1094912C9A4@cpd-exchange.ClinPath.local>, <6DB0AEDB1C984207823DB5DFA9BBC3E0@HP2010>, , , , <761E2B5697F795489C8710BCC72141FF098EDA@ex07.net.ucsf.edu> Message-ID: Me too? umbrella girls and champagne for all > From: Timothy.Morken@ucsfmedctr.org > To: jaylundgren@gmail.com; lowenjeff@hotmail.com > CC: histonet@lists.utsouthwestern.edu; cjacobs@clinpath.com > Subject: RE: [Histonet] Travel Histology Technician Jobs > Date: Tue, 10 Sep 2013 15:08:03 +0000 > > " It ain't all speedboats and supermodels, if that's what you had in mind." > > Bummer, That's exactly what I had in mind....!! > > > Tim > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jay Lundgren > Sent: Monday, September 09, 2013 5:07 PM > To: jeff lowen > Cc: histonet; Christopher Jacobs > Subject: Re: [Histonet] Travel Histology Technician Jobs > > I honestly wouldn't recommend it unless you are financially able to undergo 6 month plus periods of unemployment. In 2008 I didn't work all year, only maybe the last 3 weeks. The wages have never come back to the > pre- 2008 levels. The largest staffing agency in the country declared bankruptcy because of the downturn. > In today's economy, there is always going to be an unemployed person who is willing to work for a couple of dollars less, and this affects the agencies as well. Most of the HR depts I've dealt with lately, ONLY care about cost. As long as the applicant is JCAHO compliant and registered, they'll take the one who is 50 cents cheaper. > So, right now, the agencies are able to get people for ridiculously low wages. I won't be as crass as to post any, but they are basically asking you to subsidize your own working vacation. Usually in a semi-rural, isolated, or climatically blasted location (there's a reason they can't get a full time tech), living in the cheapest motel in town for > 3 months. When you ask the agency why they put you in that particular motel, they reply, "Because it's the cheapest place in town. (DUH)" Some agencies are better than others, but they are all SUPER cheap. The better ones are just honest about it. > I see full time jobs advertised regionally (Texas) that are offering registered techs jobs, right out of school, $4-5 more than what the agencies will offer you just starting out as a traveler, regardless of previous experience. Also, travel = NO BENEFITS. Some of the agencies pretend to have benefits, but as I said before, it's near impossible to work with enough continuity to remain covered. > Also, big warning: A lot of the "agencies" aren't really agencies at all, but someone with an 800 number and a website and some hold music. > It ain't all speedboats and supermodels, if that's what you had in mind. > > Sincerely, > > Jay A. Lundgren, M.S., HTL (ASCP) > > > On Mon, Sep 9, 2013 at 6:03 PM, jeff lowen wrote: > > > Thanks, this is very timely as I'm looking into temp/travel HT. > > Any suggestions where to look, which agencies? > > Thanks, Jeff Lowen HT/MLT(ASCP) > > > > > Date: Mon, 9 Sep 2013 17:52:34 -0500 > > > From: jaylundgren@gmail.com > > > To: lpwenk@sbcglobal.net > > > Subject: Re: [Histonet] Travel Histology Technician Jobs > > > CC: histonet@lists.utsouthwestern.edu; CJacobs@clinpath.com > > > > > > > > I've been traveling for the past 16 years! Not everyone who wanders > > > is lost. > > > > > > Sincerely, > > > > > > Jay A. Lundgren, > > > M.S., HTL (ASCP) > > > > > > > > > > > > > > > On Mon, Sep 9, 2013 at 2:00 PM, Lee & Peggy Wenk > > > > >wrote: > > > > > > > On Sunday, Sept. 22, from 8 am - 9:30 am at the NSH Symposium in > > > > Providence, RI, Beth Cox, HTL/SCT(ASCP)QIHC is presenting a > > > > workshop on "Work and Play Across the USA - A Guide to Being a Traveling Tech". > > > > http://www.histoconvention.**org/ > > > > > > > > > > Click on Schedule > > > > > > > > Peggy A. Wenk, HTL(ASCP)SLS > > > > > > > > -----Original Message----- From: Christopher Jacobs > > > > Sent: Monday, September 09, 2013 1:30 PM > > > > To: 'histonet@lists.**utsouthwestern.edu< > > histonet@lists.utsouthwestern.edu> > > > > > > ' > > > > Subject: [Histonet] Travel Histology Technician Jobs > > > > > > > > > > > > Histonetters, > > > > > > > > Does anyone out there have any information on traveling histology > > > > technician jobs? What is the compensation? How does insurance work? > > What > > > > qualifications are needed? I am particularly interested in any > > > > personal experiences any one has had. > > > > > > > > Thanks! > > > > > > > > "CJ" Christopher P. Jacobs, HT QIHC(ASCP) Clin-Path Diagnostics, > > > > LLC > > > > > > > > > > > > > > > > CONFIDENTIALITY NOTICE: This message and any attachments are for > > > > the > > sole > > > > use of the intended recipient(s). This message is confidential and > > > > may > > also > > > > be privileged. If you are not the intended recipient, please > > > > contact > > the > > > > sender immediately, delete the contents of this message and do not > > > > use > > it > > > > for any purpose. > > > > > > > > ______________________________**_________________ > > > > Histonet mailing list > > > > Histonet@lists.utsouthwestern.**edu > > > > > > > > > > http://lists.utsouthwestern.**edu/mailman/listinfo/histonet< > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > > > > > > > > ______________________________**_________________ > > > > Histonet mailing list > > > > Histonet@lists.utsouthwestern.**edu > > > > > > > > > > http://lists.utsouthwestern.**edu/mailman/listinfo/histonet< > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > > > > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From epchatfield <@t> ihis.org Tue Sep 10 10:31:52 2013 From: epchatfield <@t> ihis.org (Elizabeth Chatfield) Date: Tue Sep 10 10:32:09 2013 Subject: [Histonet] GI biopsy slides Message-ID: <522F1138020000BC0005ECF4@gwia-out.peigov> Hi folks, We are re-thinking how we cut our GI biopsy slides. Currently we are cutting 3 levels with 3 sections on each slide. Is anyone out there putting all 3 levels on the same slide? We are receiving cases with a large number of samples and some our pathologists would like to see fewer slides. Thanks, Elizabeth Charlottetown, PE ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. From jqb7 <@t> cdc.gov Tue Sep 10 10:38:13 2013 From: jqb7 <@t> cdc.gov (Sanders, Jeanine (CDC/OID/NCEZID)) Date: Tue Sep 10 10:38:20 2013 Subject: [Histonet] Travel Histology Technician Jobs In-Reply-To: References: <0082E20E79ED2245AB1DD78B87CFA1094912C9A4@cpd-exchange.ClinPath.local>, <6DB0AEDB1C984207823DB5DFA9BBC3E0@HP2010>, , , , <761E2B5697F795489C8710BCC72141FF098EDA@ex07.net.ucsf.edu> Message-ID: Or cabana boys and mojitos! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of jeff lowen Sent: Tuesday, September 10, 2013 11:11 AM To: Morken, Timothy; Jay Lundgren Cc: histonet; Christopher Jacobs Subject: RE: [Histonet] Travel Histology Technician Jobs Me too? umbrella girls and champagne for all > From: Timothy.Morken@ucsfmedctr.org > To: jaylundgren@gmail.com; lowenjeff@hotmail.com > CC: histonet@lists.utsouthwestern.edu; cjacobs@clinpath.com > Subject: RE: [Histonet] Travel Histology Technician Jobs > Date: Tue, 10 Sep 2013 15:08:03 +0000 > > " It ain't all speedboats and supermodels, if that's what you had in mind." > > Bummer, That's exactly what I had in mind....!! > > > Tim > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jay > Lundgren > Sent: Monday, September 09, 2013 5:07 PM > To: jeff lowen > Cc: histonet; Christopher Jacobs > Subject: Re: [Histonet] Travel Histology Technician Jobs > > I honestly wouldn't recommend it unless you are financially able > to undergo 6 month plus periods of unemployment. In 2008 I didn't > work all year, only maybe the last 3 weeks. The wages have never come > back to the > pre- 2008 levels. The largest staffing agency in the country declared bankruptcy because of the downturn. > In today's economy, there is always going to be an unemployed person who is willing to work for a couple of dollars less, and this affects the agencies as well. Most of the HR depts I've dealt with lately, ONLY care about cost. As long as the applicant is JCAHO compliant and registered, they'll take the one who is 50 cents cheaper. > So, right now, the agencies are able to get people for > ridiculously low wages. I won't be as crass as to post any, but they > are basically asking you to subsidize your own working vacation. > Usually in a semi-rural, isolated, or climatically blasted location > (there's a reason they can't get a full time tech), living in the > cheapest motel in town for > 3 months. When you ask the agency why they put you in that particular motel, they reply, "Because it's the cheapest place in town. (DUH)" Some agencies are better than others, but they are all SUPER cheap. The better ones are just honest about it. > I see full time jobs advertised regionally (Texas) that are offering registered techs jobs, right out of school, $4-5 more than what the agencies will offer you just starting out as a traveler, regardless of previous experience. Also, travel = NO BENEFITS. Some of the agencies pretend to have benefits, but as I said before, it's near impossible to work with enough continuity to remain covered. > Also, big warning: A lot of the "agencies" aren't really agencies at all, but someone with an 800 number and a website and some hold music. > It ain't all speedboats and supermodels, if that's what you had in mind. > > Sincerely, > > Jay A. Lundgren, M.S., HTL > (ASCP) > > > On Mon, Sep 9, 2013 at 6:03 PM, jeff lowen wrote: > > > Thanks, this is very timely as I'm looking into temp/travel HT. > > Any suggestions where to look, which agencies? > > Thanks, Jeff Lowen HT/MLT(ASCP) > > > > > Date: Mon, 9 Sep 2013 17:52:34 -0500 > > > From: jaylundgren@gmail.com > > > To: lpwenk@sbcglobal.net > > > Subject: Re: [Histonet] Travel Histology Technician Jobs > > > CC: histonet@lists.utsouthwestern.edu; CJacobs@clinpath.com > > > > > > > > I've been traveling for the past 16 years! Not everyone who > > > wanders is lost. > > > > > > Sincerely, > > > > > > Jay A. Lundgren, > > > M.S., HTL (ASCP) > > > > > > > > > > > > > > > On Mon, Sep 9, 2013 at 2:00 PM, Lee & Peggy Wenk > > > > >wrote: > > > > > > > On Sunday, Sept. 22, from 8 am - 9:30 am at the NSH Symposium in > > > > Providence, RI, Beth Cox, HTL/SCT(ASCP)QIHC is presenting a > > > > workshop on "Work and Play Across the USA - A Guide to Being a Traveling Tech". > > > > http://www.histoconvention.**org/ > > > > > > > > > > Click on Schedule > > > > > > > > Peggy A. Wenk, HTL(ASCP)SLS > > > > > > > > -----Original Message----- From: Christopher Jacobs > > > > Sent: Monday, September 09, 2013 1:30 PM > > > > To: 'histonet@lists.**utsouthwestern.edu< > > histonet@lists.utsouthwestern.edu> > > > > > > ' > > > > Subject: [Histonet] Travel Histology Technician Jobs > > > > > > > > > > > > Histonetters, > > > > > > > > Does anyone out there have any information on traveling > > > > histology technician jobs? What is the compensation? How does insurance work? > > What > > > > qualifications are needed? I am particularly interested in any > > > > personal experiences any one has had. > > > > > > > > Thanks! > > > > > > > > "CJ" Christopher P. Jacobs, HT QIHC(ASCP) Clin-Path Diagnostics, > > > > LLC > > > > > > > > > > > > > > > > CONFIDENTIALITY NOTICE: This message and any attachments are for > > > > the > > sole > > > > use of the intended recipient(s). This message is confidential > > > > and may > > also > > > > be privileged. If you are not the intended recipient, please > > > > contact > > the > > > > sender immediately, delete the contents of this message and do > > > > not use > > it > > > > for any purpose. > > > > > > > > ______________________________**_________________ > > > > Histonet mailing list > > > > Histonet@lists.utsouthwestern.**edu > > > > > > > > > > http://lists.utsouthwestern.**edu/mailman/listinfo/histonet< > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > > > > > > > > ______________________________**_________________ > > > > Histonet mailing list > > > > Histonet@lists.utsouthwestern.**edu > > > > > > > > > > http://lists.utsouthwestern.**edu/mailman/listinfo/histonet< > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > > > > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sally.norton <@t> seattlechildrens.org Tue Sep 10 10:41:59 2013 From: sally.norton <@t> seattlechildrens.org (Norton, Sally) Date: Tue Sep 10 10:42:12 2013 Subject: [Histonet] Travel Histology Technician Jobs In-Reply-To: References: <0082E20E79ED2245AB1DD78B87CFA1094912C9A4@cpd-exchange.ClinPath.local>, <6DB0AEDB1C984207823DB5DFA9BBC3E0@HP2010>, , , , <761E2B5697F795489C8710BCC72141FF098EDA@ex07.net.ucsf.edu> Message-ID: <1357F84B33D39A46BA015A8EC6ABCBD03F373483@PPWEXD01B.childrens.sea.kids> Hahaha, I'm not interested in speedboats or supermodels!! I will retire in two years and thought about this as something to do for a year or two after. Since I will have social security I can't make too much anyway. My only comment/question regarding Jay's post is - cheap motel? I've visiting nurses whose places don't look like cheap motels. Sally Norton Seattle children's Hospital -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of jeff lowen Sent: Tuesday, September 10, 2013 08:11 To: Morken, Timothy; Jay Lundgren Cc: histonet; Christopher Jacobs Subject: RE: [Histonet] Travel Histology Technician Jobs Me too? umbrella girls and champagne for all > From: Timothy.Morken@ucsfmedctr.org > To: jaylundgren@gmail.com; lowenjeff@hotmail.com > CC: histonet@lists.utsouthwestern.edu; cjacobs@clinpath.com > Subject: RE: [Histonet] Travel Histology Technician Jobs > Date: Tue, 10 Sep 2013 15:08:03 +0000 > > " It ain't all speedboats and supermodels, if that's what you had in mind." > > Bummer, That's exactly what I had in mind....!! > > > Tim > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jay Lundgren > Sent: Monday, September 09, 2013 5:07 PM > To: jeff lowen > Cc: histonet; Christopher Jacobs > Subject: Re: [Histonet] Travel Histology Technician Jobs > > I honestly wouldn't recommend it unless you are financially able to undergo 6 month plus periods of unemployment. In 2008 I didn't work all year, only maybe the last 3 weeks. The wages have never come back to the > pre- 2008 levels. The largest staffing agency in the country declared bankruptcy because of the downturn. > In today's economy, there is always going to be an unemployed person who is willing to work for a couple of dollars less, and this affects the agencies as well. Most of the HR depts I've dealt with lately, ONLY care about cost. As long as the applicant is JCAHO compliant and registered, they'll take the one who is 50 cents cheaper. > So, right now, the agencies are able to get people for ridiculously low wages. I won't be as crass as to post any, but they are basically asking you to subsidize your own working vacation. Usually in a semi-rural, isolated, or climatically blasted location (there's a reason they can't get a full time tech), living in the cheapest motel in town for > 3 months. When you ask the agency why they put you in that particular motel, they reply, "Because it's the cheapest place in town. (DUH)" Some agencies are better than others, but they are all SUPER cheap. The better ones are just honest about it. > I see full time jobs advertised regionally (Texas) that are offering registered techs jobs, right out of school, $4-5 more than what the agencies will offer you just starting out as a traveler, regardless of previous experience. Also, travel = NO BENEFITS. Some of the agencies pretend to have benefits, but as I said before, it's near impossible to work with enough continuity to remain covered. > Also, big warning: A lot of the "agencies" aren't really agencies at all, but someone with an 800 number and a website and some hold music. > It ain't all speedboats and supermodels, if that's what you had in mind. > > Sincerely, > > Jay A. Lundgren, M.S., HTL (ASCP) > > > On Mon, Sep 9, 2013 at 6:03 PM, jeff lowen wrote: > > > Thanks, this is very timely as I'm looking into temp/travel HT. > > Any suggestions where to look, which agencies? > > Thanks, Jeff Lowen HT/MLT(ASCP) > > > > > Date: Mon, 9 Sep 2013 17:52:34 -0500 > > > From: jaylundgren@gmail.com > > > To: lpwenk@sbcglobal.net > > > Subject: Re: [Histonet] Travel Histology Technician Jobs > > > CC: histonet@lists.utsouthwestern.edu; CJacobs@clinpath.com > > > > > > > > I've been traveling for the past 16 years! Not everyone who wanders > > > is lost. > > > > > > Sincerely, > > > > > > Jay A. Lundgren, > > > M.S., HTL (ASCP) > > > > > > > > > > > > > > > On Mon, Sep 9, 2013 at 2:00 PM, Lee & Peggy Wenk > > > > >wrote: > > > > > > > On Sunday, Sept. 22, from 8 am - 9:30 am at the NSH Symposium in > > > > Providence, RI, Beth Cox, HTL/SCT(ASCP)QIHC is presenting a > > > > workshop on "Work and Play Across the USA - A Guide to Being a Traveling Tech". > > > > http://www.histoconvention.**org/ > > > > > > > > > > Click on Schedule > > > > > > > > Peggy A. Wenk, HTL(ASCP)SLS > > > > > > > > -----Original Message----- From: Christopher Jacobs > > > > Sent: Monday, September 09, 2013 1:30 PM > > > > To: 'histonet@lists.**utsouthwestern.edu< > > histonet@lists.utsouthwestern.edu> > > > > > > ' > > > > Subject: [Histonet] Travel Histology Technician Jobs > > > > > > > > > > > > Histonetters, > > > > > > > > Does anyone out there have any information on traveling histology > > > > technician jobs? What is the compensation? How does insurance work? > > What > > > > qualifications are needed? I am particularly interested in any > > > > personal experiences any one has had. > > > > > > > > Thanks! > > > > > > > > "CJ" Christopher P. Jacobs, HT QIHC(ASCP) Clin-Path Diagnostics, > > > > LLC > > > > > > > > > > > > > > > > CONFIDENTIALITY NOTICE: This message and any attachments are for > > > > the > > sole > > > > use of the intended recipient(s). This message is confidential and > > > > may > > also > > > > be privileged. If you are not the intended recipient, please > > > > contact > > the > > > > sender immediately, delete the contents of this message and do not > > > > use > > it > > > > for any purpose. > > > > > > > > ______________________________**_________________ > > > > Histonet mailing list > > > > Histonet@lists.utsouthwestern.**edu > > > > > > > > > > http://lists.utsouthwestern.**edu/mailman/listinfo/histonet< > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > > > > > > > > ______________________________**_________________ > > > > Histonet mailing list > > > > Histonet@lists.utsouthwestern.**edu > > > > > > > > > > http://lists.utsouthwestern.**edu/mailman/listinfo/histonet< > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > > > > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From lowenjeff <@t> hotmail.com Tue Sep 10 10:45:04 2013 From: lowenjeff <@t> hotmail.com (jeff lowen) Date: Tue Sep 10 10:45:13 2013 Subject: [Histonet] Travel Histology Technician Jobs In-Reply-To: References: <0082E20E79ED2245AB1DD78B87CFA1094912C9A4@cpd-exchange.ClinPath.local>, , <6DB0AEDB1C984207823DB5DFA9BBC3E0@HP2010>, , , , , , , , <761E2B5697F795489C8710BCC72141FF098EDA@ex07.net.ucsf.edu>, , Message-ID: If they just let us HT's make up the rules, things would be a lot better, for us anyway? > From: jqb7@cdc.gov > To: lowenjeff@hotmail.com; timothy.morken@ucsfmedctr.org; jaylundgren@gmail.com > CC: histonet@lists.utsouthwestern.edu; cjacobs@clinpath.com > Subject: RE: [Histonet] Travel Histology Technician Jobs > Date: Tue, 10 Sep 2013 15:38:13 +0000 > > Or cabana boys and mojitos! > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of jeff lowen > Sent: Tuesday, September 10, 2013 11:11 AM > To: Morken, Timothy; Jay Lundgren > Cc: histonet; Christopher Jacobs > Subject: RE: [Histonet] Travel Histology Technician Jobs > > Me too? umbrella girls and champagne for all > > > From: Timothy.Morken@ucsfmedctr.org > > To: jaylundgren@gmail.com; lowenjeff@hotmail.com > > CC: histonet@lists.utsouthwestern.edu; cjacobs@clinpath.com > > Subject: RE: [Histonet] Travel Histology Technician Jobs > > Date: Tue, 10 Sep 2013 15:08:03 +0000 > > > > " It ain't all speedboats and supermodels, if that's what you had in mind." > > > > Bummer, That's exactly what I had in mind....!! > > > > > > Tim > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jay > > Lundgren > > Sent: Monday, September 09, 2013 5:07 PM > > To: jeff lowen > > Cc: histonet; Christopher Jacobs > > Subject: Re: [Histonet] Travel Histology Technician Jobs > > > > I honestly wouldn't recommend it unless you are financially able > > to undergo 6 month plus periods of unemployment. In 2008 I didn't > > work all year, only maybe the last 3 weeks. The wages have never come > > back to the > > pre- 2008 levels. The largest staffing agency in the country declared bankruptcy because of the downturn. > > In today's economy, there is always going to be an unemployed person who is willing to work for a couple of dollars less, and this affects the agencies as well. Most of the HR depts I've dealt with lately, ONLY care about cost. As long as the applicant is JCAHO compliant and registered, they'll take the one who is 50 cents cheaper. > > So, right now, the agencies are able to get people for > > ridiculously low wages. I won't be as crass as to post any, but they > > are basically asking you to subsidize your own working vacation. > > Usually in a semi-rural, isolated, or climatically blasted location > > (there's a reason they can't get a full time tech), living in the > > cheapest motel in town for > > 3 months. When you ask the agency why they put you in that particular motel, they reply, "Because it's the cheapest place in town. (DUH)" Some agencies are better than others, but they are all SUPER cheap. The better ones are just honest about it. > > I see full time jobs advertised regionally (Texas) that are offering registered techs jobs, right out of school, $4-5 more than what the agencies will offer you just starting out as a traveler, regardless of previous experience. Also, travel = NO BENEFITS. Some of the agencies pretend to have benefits, but as I said before, it's near impossible to work with enough continuity to remain covered. > > Also, big warning: A lot of the "agencies" aren't really agencies at all, but someone with an 800 number and a website and some hold music. > > It ain't all speedboats and supermodels, if that's what you had in mind. > > > > Sincerely, > > > > Jay A. Lundgren, M.S., HTL > > (ASCP) > > > > > > On Mon, Sep 9, 2013 at 6:03 PM, jeff lowen wrote: > > > > > Thanks, this is very timely as I'm looking into temp/travel HT. > > > Any suggestions where to look, which agencies? > > > Thanks, Jeff Lowen HT/MLT(ASCP) > > > > > > > Date: Mon, 9 Sep 2013 17:52:34 -0500 > > > > From: jaylundgren@gmail.com > > > > To: lpwenk@sbcglobal.net > > > > Subject: Re: [Histonet] Travel Histology Technician Jobs > > > > CC: histonet@lists.utsouthwestern.edu; CJacobs@clinpath.com > > > > > > > > > > > I've been traveling for the past 16 years! Not everyone who > > > > wanders is lost. > > > > > > > > Sincerely, > > > > > > > > Jay A. Lundgren, > > > > M.S., HTL (ASCP) > > > > > > > > > > > > > > > > > > > > On Mon, Sep 9, 2013 at 2:00 PM, Lee & Peggy Wenk > > > > > > >wrote: > > > > > > > > > On Sunday, Sept. 22, from 8 am - 9:30 am at the NSH Symposium in > > > > > Providence, RI, Beth Cox, HTL/SCT(ASCP)QIHC is presenting a > > > > > workshop on "Work and Play Across the USA - A Guide to Being a Traveling Tech". > > > > > http://www.histoconvention.**org/ > > > > > > > > > > > > > Click on Schedule > > > > > > > > > > Peggy A. Wenk, HTL(ASCP)SLS > > > > > > > > > > -----Original Message----- From: Christopher Jacobs > > > > > Sent: Monday, September 09, 2013 1:30 PM > > > > > To: 'histonet@lists.**utsouthwestern.edu< > > > histonet@lists.utsouthwestern.edu> > > > > > > > > ' > > > > > Subject: [Histonet] Travel Histology Technician Jobs > > > > > > > > > > > > > > > Histonetters, > > > > > > > > > > Does anyone out there have any information on traveling > > > > > histology technician jobs? What is the compensation? How does insurance work? > > > What > > > > > qualifications are needed? I am particularly interested in any > > > > > personal experiences any one has had. > > > > > > > > > > Thanks! > > > > > > > > > > "CJ" Christopher P. Jacobs, HT QIHC(ASCP) Clin-Path Diagnostics, > > > > > LLC > > > > > > > > > > > > > > > > > > > > CONFIDENTIALITY NOTICE: This message and any attachments are for > > > > > the > > > sole > > > > > use of the intended recipient(s). This message is confidential > > > > > and may > > > also > > > > > be privileged. If you are not the intended recipient, please > > > > > contact > > > the > > > > > sender immediately, delete the contents of this message and do > > > > > not use > > > it > > > > > for any purpose. > > > > > > > > > > ______________________________**_________________ > > > > > Histonet mailing list > > > > > Histonet@lists.utsouthwestern.**edu > > > > > > > > > > > > > http://lists.utsouthwestern.**edu/mailman/listinfo/histonet< > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > > > > > > > > > > ______________________________**_________________ > > > > > Histonet mailing list > > > > > Histonet@lists.utsouthwestern.**edu > > > > > > > > > > > > > http://lists.utsouthwestern.**edu/mailman/listinfo/histonet< > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > > > > > > > > > > > > _______________________________________________ > > > > Histonet mailing list > > > > Histonet@lists.utsouthwestern.edu > > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Joyce.Weems <@t> emoryhealthcare.org Tue Sep 10 10:47:35 2013 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Tue Sep 10 10:47:47 2013 Subject: [Histonet] GI biopsy slides In-Reply-To: <522F1138020000BC0005ECF4@gwia-out.peigov> References: <522F1138020000BC0005ECF4@gwia-out.peigov> Message-ID: We put 3 levels on the same slide when specimen size allows. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chatfield Sent: Tuesday, September 10, 2013 11:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] GI biopsy slides Hi folks, We are re-thinking how we cut our GI biopsy slides. Currently we are cutting 3 levels with 3 sections on each slide. Is anyone out there putting all 3 levels on the same slide? We are receiving cases with a large number of samples and some our pathologists would like to see fewer slides. Thanks, Elizabeth Charlottetown, PE ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From micropathlabs <@t> yahoo.com Tue Sep 10 10:58:36 2013 From: micropathlabs <@t> yahoo.com (Sheila Haas) Date: Tue Sep 10 10:58:45 2013 Subject: [Histonet] GI biopsy slides In-Reply-To: <522F1138020000BC0005ECF4@gwia-out.peigov> References: <522F1138020000BC0005ECF4@gwia-out.peigov> Message-ID: <1378828716.86989.YahooMailNeo@web122002.mail.ne1.yahoo.com> We cut 3 levels on one slide. Each level typically has two sections across the slide. If there are multiple pieces of tissue that need to be embedded in a little larger mold, we?put 3 levels on 1 slide by breaking away the excess paraffin around the sections while they're on the waterbath. Then we can then fit 3? levels on 1 slide but each level will only have 1 section.?Hope this makes sense. Sheila Haas Laboratory Manager MicroPath Laboratories, Inc. ________________________________ From: Elizabeth Chatfield To: histonet@lists.utsouthwestern.edu Sent: Tuesday, September 10, 2013 11:31 AM Subject: [Histonet] GI biopsy slides Hi folks, We are re-thinking how we cut our GI biopsy slides. Currently we are cutting 3 levels with 3 sections on each slide.? Is anyone out there putting all 3 levels on the same slide?? We are receiving cases with a large number of samples and some our pathologists would like to see fewer slides.? Thanks, Elizabeth Charlottetown, PE ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sally.norton <@t> seattlechildrens.org Tue Sep 10 11:02:22 2013 From: sally.norton <@t> seattlechildrens.org (Norton, Sally) Date: Tue Sep 10 11:02:33 2013 Subject: [Histonet] GI biopsy slides In-Reply-To: References: <522F1138020000BC0005ECF4@gwia-out.peigov> Message-ID: <1357F84B33D39A46BA015A8EC6ABCBD03F3734A3@PPWEXD01B.childrens.sea.kids> Are your specimens large? We cut two levels, with two ribbons on each slide. Sally Norton Seattle children's Hospital -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Tuesday, September 10, 2013 08:48 To: 'Elizabeth Chatfield'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] GI biopsy slides We put 3 levels on the same slide when specimen size allows. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chatfield Sent: Tuesday, September 10, 2013 11:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] GI biopsy slides Hi folks, We are re-thinking how we cut our GI biopsy slides. Currently we are cutting 3 levels with 3 sections on each slide. Is anyone out there putting all 3 levels on the same slide? We are receiving cases with a large number of samples and some our pathologists would like to see fewer slides. Thanks, Elizabeth Charlottetown, PE ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From lcolbert <@t> pathmdlabs.com Tue Sep 10 12:18:52 2013 From: lcolbert <@t> pathmdlabs.com (Laurie Colbert) Date: Tue Sep 10 12:22:20 2013 Subject: [Histonet] GI biopsy slides In-Reply-To: <1378828716.86989.YahooMailNeo@web122002.mail.ne1.yahoo.com> References: <522F1138020000BC0005ECF4@gwia-out.peigov> <1378828716.86989.YahooMailNeo@web122002.mail.ne1.yahoo.com> Message-ID: <12ECD7346266D74691EC2BFC75285E452F33E7AD@BFL323E10.pathmdlabs.local> We cut exactly as Sheila does. Laurie Colbert, HT (ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Haas Sent: Tuesday, September 10, 2013 8:59 AM To: Elizabeth Chatfield; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] GI biopsy slides We cut 3 levels on one slide. Each level typically has two sections across the slide. If there are multiple pieces of tissue that need to be embedded in a little larger mold, we?put 3 levels on 1 slide by breaking away the excess paraffin around the sections while they're on the waterbath. Then we can then fit 3? levels on 1 slide but each level will only have 1 section.?Hope this makes sense. Sheila Haas Laboratory Manager MicroPath Laboratories, Inc. ________________________________ From: Elizabeth Chatfield To: histonet@lists.utsouthwestern.edu Sent: Tuesday, September 10, 2013 11:31 AM Subject: [Histonet] GI biopsy slides Hi folks, We are re-thinking how we cut our GI biopsy slides. Currently we are cutting 3 levels with 3 sections on each slide.? Is anyone out there putting all 3 levels on the same slide?? We are receiving cases with a large number of samples and some our pathologists would like to see fewer slides.? Thanks, Elizabeth Charlottetown, PE ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kkienitz <@t> orclinic.com Tue Sep 10 12:30:40 2013 From: kkienitz <@t> orclinic.com (Kienitz, Kari) Date: Tue Sep 10 12:32:41 2013 Subject: [Histonet] GI biopsy slides In-Reply-To: <12ECD7346266D74691EC2BFC75285E452F33E7AD@BFL323E10.pathmdlabs.local> References: <522F1138020000BC0005ECF4@gwia-out.peigov> <1378828716.86989.YahooMailNeo@web122002.mail.ne1.yahoo.com>, <12ECD7346266D74691EC2BFC75285E452F33E7AD@BFL323E10.pathmdlabs.local> Message-ID: <41400FFE517878449D89114DD252609008A2E40CE4@tocmail1.tocad.orclinic.com> My GI Pathologists require no more than 5 pieces of tissue per block; three levels on one slide; one section per level. Kari Kienitz HT, (ASCP) Histology Laboratory Portland Gastroenterology The Oregon Clinic 1111 NE 99th Ave Portland, OR 97220 503.935.8311 kkienitz@orclinic.com CONFIDENTIALITY WARNING: This e-mail and any attachments are for the exclusive and confidential use of the intended recipient. If you are not the intended recipient, please do not read, distribute or take action in reliance upon this missive. If you have received this in error, please notify the sender immediately by reply e-mail and delete this message and its attachments from your computer system. Thank you ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert [lcolbert@pathmdlabs.com] Sent: Tuesday, September 10, 2013 10:18 AM To: Sheila Haas; Elizabeth Chatfield; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] GI biopsy slides We cut exactly as Sheila does. Laurie Colbert, HT (ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Haas Sent: Tuesday, September 10, 2013 8:59 AM To: Elizabeth Chatfield; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] GI biopsy slides We cut 3 levels on one slide. Each level typically has two sections across the slide. If there are multiple pieces of tissue that need to be embedded in a little larger mold, we put 3 levels on 1 slide by breaking away the excess paraffin around the sections while they're on the waterbath. Then we can then fit 3 levels on 1 slide but each level will only have 1 section. Hope this makes sense. Sheila Haas Laboratory Manager MicroPath Laboratories, Inc. ________________________________ From: Elizabeth Chatfield To: histonet@lists.utsouthwestern.edu Sent: Tuesday, September 10, 2013 11:31 AM Subject: [Histonet] GI biopsy slides Hi folks, We are re-thinking how we cut our GI biopsy slides. Currently we are cutting 3 levels with 3 sections on each slide. Is anyone out there putting all 3 levels on the same slide? We are receiving cases with a large number of samples and some our pathologists would like to see fewer slides. Thanks, Elizabeth Charlottetown, PE ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From trathborne <@t> somerset-healthcare.com Tue Sep 10 12:50:26 2013 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Tue Sep 10 12:51:10 2013 Subject: [Histonet] GI biopsy slides In-Reply-To: <522F1138020000BC0005ECF4@gwia-out.peigov> References: <522F1138020000BC0005ECF4@gwia-out.peigov> Message-ID: <3AD061FE740D464FAC7BF6B5CFB75707A8A5A95E@smcmail02.somerset-healthcare.com> We've been doing this for years. You'd be surprised with the overall savings (slides, frequency of changing stains, storage), and also TAT since you'll be able to put through more cases on your stainer. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chatfield Sent: Tuesday, September 10, 2013 11:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] GI biopsy slides Hi folks, We are re-thinking how we cut our GI biopsy slides. Currently we are cutting 3 levels with 3 sections on each slide. Is anyone out there putting all 3 levels on the same slide? We are receiving cases with a large number of samples and some our pathologists would like to see fewer slides. Thanks, Elizabeth Charlottetown, PE ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. From Michelle.Dragoo <@t> PresenceHealth.org Tue Sep 10 12:54:18 2013 From: Michelle.Dragoo <@t> PresenceHealth.org (Dragoo, Michelle) Date: Tue Sep 10 12:55:03 2013 Subject: [Histonet] GI biopsy slides References: <178fc90d-8a3d-4ff7-8a22-2cb7f506f535@rlicexhub2v.reshealthcare.org> Message-ID: <51555C8A50338F47A9E589D853081A720374584F@AEXMV12.phnet.phroot.local> We put all 3 levels on one slide as well. Michelle R. Dragoo, MBA, HT (ASCP) Histology Coordinator Alverno Provena Hospital Laboratories Presence Saint Joseph Hospital - Elgin 77 N Airlite St | Elgin, IL 60123 T: 847.695.3200 x5309 | F: 847.888.3318 Michelle.Dragoo@presencehealth.org ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of histonet-request@lists.utsouthwestern.edu Sent: Tue 9/10/2013 12:04 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 118, Issue 14 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: Travel Histology Technician Jobs (jeff lowen) 2. RE: GI biopsy slides (Weems, Joyce K.) 3. Re: GI biopsy slides (Sheila Haas) 4. RE: GI biopsy slides (Norton, Sally) ---------------------------------------------------------------------- Message: 1 Date: Tue, 10 Sep 2013 08:45:04 -0700 From: jeff lowen Subject: RE: [Histonet] Travel Histology Technician Jobs To: "Sanders, Jeanine (CDCOIDNCEZID)" , "Morken, Timothy" , Jay Lundgren Cc: histonet , Christopher Jacobs Message-ID: Content-Type: text/plain; charset="iso-8859-1" If they just let us HT's make up the rules, things would be a lot better, for us anyway? > From: jqb7@cdc.gov > To: lowenjeff@hotmail.com; timothy.morken@ucsfmedctr.org; jaylundgren@gmail.com > CC: histonet@lists.utsouthwestern.edu; cjacobs@clinpath.com > Subject: RE: [Histonet] Travel Histology Technician Jobs > Date: Tue, 10 Sep 2013 15:38:13 +0000 > > Or cabana boys and mojitos! > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of jeff lowen > Sent: Tuesday, September 10, 2013 11:11 AM > To: Morken, Timothy; Jay Lundgren > Cc: histonet; Christopher Jacobs > Subject: RE: [Histonet] Travel Histology Technician Jobs > > Me too? umbrella girls and champagne for all > > > From: Timothy.Morken@ucsfmedctr.org > > To: jaylundgren@gmail.com; lowenjeff@hotmail.com > > CC: histonet@lists.utsouthwestern.edu; cjacobs@clinpath.com > > Subject: RE: [Histonet] Travel Histology Technician Jobs > > Date: Tue, 10 Sep 2013 15:08:03 +0000 > > > > " It ain't all speedboats and supermodels, if that's what you had in mind." > > > > Bummer, That's exactly what I had in mind....!! > > > > > > Tim > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jay > > Lundgren > > Sent: Monday, September 09, 2013 5:07 PM > > To: jeff lowen > > Cc: histonet; Christopher Jacobs > > Subject: Re: [Histonet] Travel Histology Technician Jobs > > > > I honestly wouldn't recommend it unless you are financially able > > to undergo 6 month plus periods of unemployment. In 2008 I didn't > > work all year, only maybe the last 3 weeks. The wages have never come > > back to the > > pre- 2008 levels. The largest staffing agency in the country declared bankruptcy because of the downturn. > > In today's economy, there is always going to be an unemployed person who is willing to work for a couple of dollars less, and this affects the agencies as well. Most of the HR depts I've dealt with lately, ONLY care about cost. As long as the applicant is JCAHO compliant and registered, they'll take the one who is 50 cents cheaper. > > So, right now, the agencies are able to get people for > > ridiculously low wages. I won't be as crass as to post any, but they > > are basically asking you to subsidize your own working vacation. > > Usually in a semi-rural, isolated, or climatically blasted location > > (there's a reason they can't get a full time tech), living in the > > cheapest motel in town for > > 3 months. When you ask the agency why they put you in that particular motel, they reply, "Because it's the cheapest place in town. (DUH)" Some agencies are better than others, but they are all SUPER cheap. The better ones are just honest about it. > > I see full time jobs advertised regionally (Texas) that are offering registered techs jobs, right out of school, $4-5 more than what the agencies will offer you just starting out as a traveler, regardless of previous experience. Also, travel = NO BENEFITS. Some of the agencies pretend to have benefits, but as I said before, it's near impossible to work with enough continuity to remain covered. > > Also, big warning: A lot of the "agencies" aren't really agencies at all, but someone with an 800 number and a website and some hold music. > > It ain't all speedboats and supermodels, if that's what you had in mind. > > > > Sincerely, > > > > Jay A. Lundgren, M.S., HTL > > (ASCP) > > > > > > On Mon, Sep 9, 2013 at 6:03 PM, jeff lowen wrote: > > > > > Thanks, this is very timely as I'm looking into temp/travel HT. > > > Any suggestions where to look, which agencies? > > > Thanks, Jeff Lowen HT/MLT(ASCP) > > > > > > > Date: Mon, 9 Sep 2013 17:52:34 -0500 > > > > From: jaylundgren@gmail.com > > > > To: lpwenk@sbcglobal.net > > > > Subject: Re: [Histonet] Travel Histology Technician Jobs > > > > CC: histonet@lists.utsouthwestern.edu; CJacobs@clinpath.com > > > > > > > > > > > I've been traveling for the past 16 years! Not everyone who > > > > wanders is lost. > > > > > > > > Sincerely, > > > > > > > > Jay A. Lundgren, > > > > M.S., HTL (ASCP) > > > > > > > > > > > > > > > > > > > > On Mon, Sep 9, 2013 at 2:00 PM, Lee & Peggy Wenk > > > > > > >wrote: > > > > > > > > > On Sunday, Sept. 22, from 8 am - 9:30 am at the NSH Symposium in > > > > > Providence, RI, Beth Cox, HTL/SCT(ASCP)QIHC is presenting a > > > > > workshop on "Work and Play Across the USA - A Guide to Being a Traveling Tech". > > > > > http://www.histoconvention.**org/ > > > > > > > > > > > > > Click on Schedule > > > > > > > > > > Peggy A. Wenk, HTL(ASCP)SLS > > > > > > > > > > -----Original Message----- From: Christopher Jacobs > > > > > Sent: Monday, September 09, 2013 1:30 PM > > > > > To: 'histonet@lists.**utsouthwestern.edu< > > > histonet@lists.utsouthwestern.edu> > > > > > > > > ' > > > > > Subject: [Histonet] Travel Histology Technician Jobs > > > > > > > > > > > > > > > Histonetters, > > > > > > > > > > Does anyone out there have any information on traveling > > > > > histology technician jobs? What is the compensation? How does insurance work? > > > What > > > > > qualifications are needed? I am particularly interested in any > > > > > personal experiences any one has had. > > > > > > > > > > Thanks! > > > > > > > > > > "CJ" Christopher P. Jacobs, HT QIHC(ASCP) Clin-Path Diagnostics, > > > > > LLC > > > > > > > > > > > > > > > > > > > > CONFIDENTIALITY NOTICE: This message and any attachments are for > > > > > the > > > sole > > > > > use of the intended recipient(s). This message is confidential > > > > > and may > > > also > > > > > be privileged. If you are not the intended recipient, please > > > > > contact > > > the > > > > > sender immediately, delete the contents of this message and do > > > > > not use > > > it > > > > > for any purpose. > > > > > > > > > > ______________________________**_________________ > > > > > Histonet mailing list > > > > > Histonet@lists.utsouthwestern.**edu > > > > > > > > > > > > > http://lists.utsouthwestern.**edu/mailman/listinfo/histonet< > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > > > > > > > > > > ______________________________**_________________ > > > > > Histonet mailing list > > > > > Histonet@lists.utsouthwestern.**edu > > > > > > > > > > > > > http://lists.utsouthwestern.**edu/mailman/listinfo/histonet< > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > > > > > > > > > > > > _______________________________________________ > > > > Histonet mailing list > > > > Histonet@lists.utsouthwestern.edu > > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Tue, 10 Sep 2013 15:47:35 +0000 From: "Weems, Joyce K." Subject: RE: [Histonet] GI biopsy slides To: "'Elizabeth Chatfield'" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" We put 3 levels on the same slide when specimen size allows. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chatfield Sent: Tuesday, September 10, 2013 11:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] GI biopsy slides Hi folks, We are re-thinking how we cut our GI biopsy slides. Currently we are cutting 3 levels with 3 sections on each slide. Is anyone out there putting all 3 levels on the same slide? We are receiving cases with a large number of samples and some our pathologists would like to see fewer slides. Thanks, Elizabeth Charlottetown, PE ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). ------------------------------ Message: 3 Date: Tue, 10 Sep 2013 08:58:36 -0700 (PDT) From: Sheila Haas Subject: Re: [Histonet] GI biopsy slides To: Elizabeth Chatfield , "histonet@lists.utsouthwestern.edu" Message-ID: <1378828716.86989.YahooMailNeo@web122002.mail.ne1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 We cut 3 levels on one slide. Each level typically has two sections across the slide. If there are multiple pieces of tissue that need to be embedded in a little larger mold, we put 3 levels on 1 slide by breaking away the excess paraffin around the sections while they're on the waterbath. Then we can then fit 3 levels on 1 slide but each level will only have 1 section. Hope this makes sense. Sheila Haas Laboratory Manager MicroPath Laboratories, Inc. ________________________________ From: Elizabeth Chatfield To: histonet@lists.utsouthwestern.edu Sent: Tuesday, September 10, 2013 11:31 AM Subject: [Histonet] GI biopsy slides Hi folks, We are re-thinking how we cut our GI biopsy slides. Currently we are cutting 3 levels with 3 sections on each slide. Is anyone out there putting all 3 levels on the same slide? We are receiving cases with a large number of samples and some our pathologists would like to see fewer slides. Thanks, Elizabeth Charlottetown, PE ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Tue, 10 Sep 2013 16:02:22 +0000 From: "Norton, Sally" Subject: RE: [Histonet] GI biopsy slides To: "'Weems, Joyce K.'" , 'Elizabeth Chatfield' , "histonet@lists.utsouthwestern.edu" Message-ID: <1357F84B33D39A46BA015A8EC6ABCBD03F3734A3@PPWEXD01B.childrens.sea.kids> Content-Type: text/plain; charset="us-ascii" Are your specimens large? We cut two levels, with two ribbons on each slide. Sally Norton Seattle children's Hospital -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Tuesday, September 10, 2013 08:48 To: 'Elizabeth Chatfield'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] GI biopsy slides We put 3 levels on the same slide when specimen size allows. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chatfield Sent: Tuesday, September 10, 2013 11:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] GI biopsy slides Hi folks, We are re-thinking how we cut our GI biopsy slides. Currently we are cutting 3 levels with 3 sections on each slide. Is anyone out there putting all 3 levels on the same slide? We are receiving cases with a large number of samples and some our pathologists would like to see fewer slides. Thanks, Elizabeth Charlottetown, PE ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 118, Issue 14 ***************************************** From lowenjeff <@t> hotmail.com Tue Sep 10 12:57:23 2013 From: lowenjeff <@t> hotmail.com (jeff lowen) Date: Tue Sep 10 12:57:28 2013 Subject: [Histonet] GI biopsy slides In-Reply-To: <41400FFE517878449D89114DD252609008A2E40CE4@tocmail1.tocad.orclinic.com> References: <522F1138020000BC0005ECF4@gwia-out.peigov>, <1378828716.86989.YahooMailNeo@web122002.mail.ne1.yahoo.com>, , <12ECD7346266D74691EC2BFC75285E452F33E7AD@BFL323E10.pathmdlabs.local>, <41400FFE517878449D89114DD252609008A2E40CE4@tocmail1.tocad.orclinic.com> Message-ID: That would make it easier to embed though don't u end up with multiple blocks and the additional slides that way at times? > From: kkienitz@orclinic.com > To: lcolbert@pathmdlabs.com; micropathlabs@yahoo.com; epchatfield@ihis.org; histonet@lists.utsouthwestern.edu > Date: Tue, 10 Sep 2013 10:30:40 -0700 > Subject: RE: [Histonet] GI biopsy slides > CC: > > My GI Pathologists require no more than 5 pieces of tissue per block; three levels on one slide; one section per level. > > > Kari Kienitz HT, (ASCP) > Histology Laboratory > Portland Gastroenterology > The Oregon Clinic > 1111 NE 99th Ave > Portland, OR 97220 > 503.935.8311 > kkienitz@orclinic.com > > > > > CONFIDENTIALITY WARNING: This e-mail and any attachments are for the exclusive and confidential use of the intended recipient. If you are not the intended recipient, please do not read, distribute or take action in reliance upon this missive. If you have received this in error, please notify the sender immediately by reply e-mail and delete this message and its attachments from your computer system. Thank you > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert [lcolbert@pathmdlabs.com] > Sent: Tuesday, September 10, 2013 10:18 AM > To: Sheila Haas; Elizabeth Chatfield; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] GI biopsy slides > > We cut exactly as Sheila does. > > Laurie Colbert, HT (ASCP) > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Haas > Sent: Tuesday, September 10, 2013 8:59 AM > To: Elizabeth Chatfield; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] GI biopsy slides > > We cut 3 levels on one slide. Each level typically has two sections across the slide. If there are multiple pieces of tissue that need to be embedded in a little larger mold, we put 3 levels on 1 slide by breaking away the excess paraffin around the sections while they're on the waterbath. Then we can then fit 3 levels on 1 slide but each level will only have 1 section. Hope this makes sense. > > > Sheila Haas > Laboratory Manager > MicroPath Laboratories, Inc. > > > ________________________________ > From: Elizabeth Chatfield > To: histonet@lists.utsouthwestern.edu > Sent: Tuesday, September 10, 2013 11:31 AM > Subject: [Histonet] GI biopsy slides > > > Hi folks, > > We are re-thinking how we cut our GI biopsy slides. Currently we are cutting 3 levels with 3 sections on each slide. Is anyone out there putting all 3 levels on the same slide? We are receiving cases with a large number of samples and some our pathologists would like to see fewer slides. > > > Thanks, > Elizabeth > Charlottetown, PE > > ------------------------- > Statement of Confidentiality > This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kkienitz <@t> orclinic.com Tue Sep 10 13:37:08 2013 From: kkienitz <@t> orclinic.com (Kienitz, Kari) Date: Tue Sep 10 13:38:33 2013 Subject: [Histonet] GI biopsy slides In-Reply-To: References: <522F1138020000BC0005ECF4@gwia-out.peigov>, <1378828716.86989.YahooMailNeo@web122002.mail.ne1.yahoo.com>, , <12ECD7346266D74691EC2BFC75285E452F33E7AD@BFL323E10.pathmdlabs.local>, <41400FFE517878449D89114DD252609008A2E40CE4@tocmail1.tocad.orclinic.com>, Message-ID: <41400FFE517878449D89114DD252609008A2E40CE6@tocmail1.tocad.orclinic.com> yes....for some cases there are many blocks and slides! Kari Kienitz HT, (ASCP) Histology Laboratory Portland Gastroenterology The Oregon Clinic 1111 NE 99th Ave Portland, OR 97220 503.935.8311 kkienitz@orclinic.com CONFIDENTIALITY WARNING: This e-mail and any attachments are for the exclusive and confidential use of the intended recipient. If you are not the intended recipient, please do not read, distribute or take action in reliance upon this missive. If you have received this in error, please notify the sender immediately by reply e-mail and delete this message and its attachments from your computer system. Thank you ________________________________ From: jeff lowen [lowenjeff@hotmail.com] Sent: Tuesday, September 10, 2013 10:57 AM To: Kienitz, Kari; Laurie Colbert; Sheila Haas; Elizabeth Chatfield; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] GI biopsy slides That would make it easier to embed though don't u end up with multiple blocks and the additional slides that way at times? > From: kkienitz@orclinic.com > To: lcolbert@pathmdlabs.com; micropathlabs@yahoo.com; epchatfield@ihis.org; histonet@lists.utsouthwestern.edu > Date: Tue, 10 Sep 2013 10:30:40 -0700 > Subject: RE: [Histonet] GI biopsy slides > CC: > > My GI Pathologists require no more than 5 pieces of tissue per block; three levels on one slide; one section per level. > > > Kari Kienitz HT, (ASCP) > Histology Laboratory > Portland Gastroenterology > The Oregon Clinic > 1111 NE 99th Ave > Portland, OR 97220 > 503.935.8311 > kkienitz@orclinic.com > > > > > CONFIDENTIALITY WARNING: This e-mail and any attachments are for the exclusive and confidential use of the intended recipient. If you are not the intended recipient, please do not read, distribute or take action in reliance upon this missive. If you have received this in error, please notify the sender immediately by reply e-mail and delete this message and its attachments from your computer system. Thank you > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert [lcolbert@pathmdlabs.com] > Sent: Tuesday, September 10, 2013 10:18 AM > To: Sheila Haas; Elizabeth Chatfield; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] GI biopsy slides > > We cut exactly as Sheila does. > > Laurie Colbert, HT (ASCP) > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Haas > Sent: Tuesday, September 10, 2013 8:59 AM > To: Elizabeth Chatfield; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] GI biopsy slides > > We cut 3 levels on one slide. Each level typically has two sections across the slide. If there are multiple pieces of tissue that need to be embedded in a little larger mold, we put 3 levels on 1 slide by breaking away the excess paraffin around the sections while they're on the waterbath. Then we can then fit 3 levels on 1 slide but each level will only have 1 section. Hope this makes sense. > > > Sheila Haas > Laboratory Manager > MicroPath Laboratories, Inc. > > > ________________________________ > From: Elizabeth Chatfield > To: histonet@lists.utsouthwestern.edu > Sent: Tuesday, September 10, 2013 11:31 AM > Subject: [Histonet] GI biopsy slides > > > Hi folks, > > We are re-thinking how we cut our GI biopsy slides. Currently we are cutting 3 levels with 3 sections on each slide. Is anyone out there putting all 3 levels on the same slide? We are receiving cases with a large number of samples and some our pathologists would like to see fewer slides. > > > Thanks, > Elizabeth > Charlottetown, PE > > ------------------------- > Statement of Confidentiality > This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Andrea.X.Dejager <@t> kp.org Tue Sep 10 14:21:32 2013 From: Andrea.X.Dejager <@t> kp.org (Andrea.X.Dejager@kp.org) Date: Tue Sep 10 14:22:07 2013 Subject: [Histonet] Re: Histonet Digest, Vol 118, Issue 14 In-Reply-To: <201309101702.r8AH2gxf006807@imp6.kp.org> References: <201309101702.r8AH2gxf006807@imp6.kp.org> Message-ID: We routinely cut 3 levels on 1 slide for almost all specimens. This makes it easier for all, pathologists, techs and just imagine cost savings for slides. Andrea De Jager, H.T. ASCP Histology Manager, Regional Reference Lab Kaiser Permanente - Colorado Phone: 303-404-4152 Fax: 303-404-4161 email: ANDREA.X.DEJAGER@KP.org NOTICE TO RECIPIENT: If you are not the intended recipient of this e-mail, you are prohibited from sharing, copying, or otherwise using or disclosing its contents. If you have received this e-mail in error, please notify the sender immediately by reply e-mail and permanently delete this e-mail and any attachments without reading, forwarding or saving them. Thank you. From: histonet-request@lists.utsouthwestern.edu To: histonet@lists.utsouthwestern.edu Date: 09/10/2013 11:03 AM Subject: Histonet Digest, Vol 118, Issue 14 Sent by: histonet-bounces@lists.utsouthwestern.edu Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: Travel Histology Technician Jobs (jeff lowen) 2. RE: GI biopsy slides (Weems, Joyce K.) 3. Re: GI biopsy slides (Sheila Haas) 4. RE: GI biopsy slides (Norton, Sally) ---------------------------------------------------------------------- Message: 1 Date: Tue, 10 Sep 2013 08:45:04 -0700 From: jeff lowen Subject: RE: [Histonet] Travel Histology Technician Jobs To: "Sanders, Jeanine (CDCOIDNCEZID)" , "Morken, Timothy" , Jay Lundgren Cc: histonet , Christopher Jacobs Message-ID: Content-Type: text/plain; charset="iso-8859-1" If they just let us HT's make up the rules, things would be a lot better, for us anyway? > From: jqb7@cdc.gov > To: lowenjeff@hotmail.com; timothy.morken@ucsfmedctr.org; jaylundgren@gmail.com > CC: histonet@lists.utsouthwestern.edu; cjacobs@clinpath.com > Subject: RE: [Histonet] Travel Histology Technician Jobs > Date: Tue, 10 Sep 2013 15:38:13 +0000 > > Or cabana boys and mojitos! > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [ mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of jeff lowen > Sent: Tuesday, September 10, 2013 11:11 AM > To: Morken, Timothy; Jay Lundgren > Cc: histonet; Christopher Jacobs > Subject: RE: [Histonet] Travel Histology Technician Jobs > > Me too? umbrella girls and champagne for all > > > From: Timothy.Morken@ucsfmedctr.org > > To: jaylundgren@gmail.com; lowenjeff@hotmail.com > > CC: histonet@lists.utsouthwestern.edu; cjacobs@clinpath.com > > Subject: RE: [Histonet] Travel Histology Technician Jobs > > Date: Tue, 10 Sep 2013 15:08:03 +0000 > > > > " It ain't all speedboats and supermodels, if that's what you had in mind." > > > > Bummer, That's exactly what I had in mind....!! > > > > > > Tim > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jay > > Lundgren > > Sent: Monday, September 09, 2013 5:07 PM > > To: jeff lowen > > Cc: histonet; Christopher Jacobs > > Subject: Re: [Histonet] Travel Histology Technician Jobs > > > > I honestly wouldn't recommend it unless you are financially able > > to undergo 6 month plus periods of unemployment. In 2008 I didn't > > work all year, only maybe the last 3 weeks. The wages have never come > > back to the > > pre- 2008 levels. The largest staffing agency in the country declared bankruptcy because of the downturn. > > In today's economy, there is always going to be an unemployed person who is willing to work for a couple of dollars less, and this affects the agencies as well. Most of the HR depts I've dealt with lately, ONLY care about cost. As long as the applicant is JCAHO compliant and registered, they'll take the one who is 50 cents cheaper. > > So, right now, the agencies are able to get people for > > ridiculously low wages. I won't be as crass as to post any, but they > > are basically asking you to subsidize your own working vacation. > > Usually in a semi-rural, isolated, or climatically blasted location > > (there's a reason they can't get a full time tech), living in the > > cheapest motel in town for > > 3 months. When you ask the agency why they put you in that particular motel, they reply, "Because it's the cheapest place in town. (DUH)" Some agencies are better than others, but they are all SUPER cheap. The better ones are just honest about it. > > I see full time jobs advertised regionally (Texas) that are offering registered techs jobs, right out of school, $4-5 more than what the agencies will offer you just starting out as a traveler, regardless of previous experience. Also, travel = NO BENEFITS. Some of the agencies pretend to have benefits, but as I said before, it's near impossible to work with enough continuity to remain covered. > > Also, big warning: A lot of the "agencies" aren't really agencies at all, but someone with an 800 number and a website and some hold music. > > It ain't all speedboats and supermodels, if that's what you had in mind. > > > > Sincerely, > > > > Jay A. Lundgren, M.S., HTL > > (ASCP) > > > > > > On Mon, Sep 9, 2013 at 6:03 PM, jeff lowen wrote: > > > > > Thanks, this is very timely as I'm looking into temp/travel HT. > > > Any suggestions where to look, which agencies? > > > Thanks, Jeff Lowen HT/MLT(ASCP) > > > > > > > Date: Mon, 9 Sep 2013 17:52:34 -0500 > > > > From: jaylundgren@gmail.com > > > > To: lpwenk@sbcglobal.net > > > > Subject: Re: [Histonet] Travel Histology Technician Jobs > > > > CC: histonet@lists.utsouthwestern.edu; CJacobs@clinpath.com > > > > > > > > > > > I've been traveling for the past 16 years! Not everyone who > > > > wanders is lost. > > > > > > > > Sincerely, > > > > > > > > Jay A. Lundgren, > > > > M.S., HTL (ASCP) > > > > > > > > > > > > > > > > > > > > On Mon, Sep 9, 2013 at 2:00 PM, Lee & Peggy Wenk > > > > > > >wrote: > > > > > > > > > On Sunday, Sept. 22, from 8 am - 9:30 am at the NSH Symposium in > > > > > Providence, RI, Beth Cox, HTL/SCT(ASCP)QIHC is presenting a > > > > > workshop on "Work and Play Across the USA - A Guide to Being a Traveling Tech". > > > > > http://www.histoconvention.**org/ > > > > > > > > > > > > > Click on Schedule > > > > > > > > > > Peggy A. Wenk, HTL(ASCP)SLS > > > > > > > > > > -----Original Message----- From: Christopher Jacobs > > > > > Sent: Monday, September 09, 2013 1:30 PM > > > > > To: 'histonet@lists.**utsouthwestern.edu< > > > histonet@lists.utsouthwestern.edu> > > > > > > > > ' > > > > > Subject: [Histonet] Travel Histology Technician Jobs > > > > > > > > > > > > > > > Histonetters, > > > > > > > > > > Does anyone out there have any information on traveling > > > > > histology technician jobs? What is the compensation? How does insurance work? > > > What > > > > > qualifications are needed? I am particularly interested in any > > > > > personal experiences any one has had. > > > > > > > > > > Thanks! > > > > > > > > > > "CJ" Christopher P. Jacobs, HT QIHC(ASCP) Clin-Path Diagnostics, > > > > > LLC > > > > > > > > > > > > > > > > > > > > CONFIDENTIALITY NOTICE: This message and any attachments are for > > > > > the > > > sole > > > > > use of the intended recipient(s). This message is confidential > > > > > and may > > > also > > > > > be privileged. If you are not the intended recipient, please > > > > > contact > > > the > > > > > sender immediately, delete the contents of this message and do > > > > > not use > > > it > > > > > for any purpose. > > > > > > > > > > ______________________________**_________________ > > > > > Histonet mailing list > > > > > Histonet@lists.utsouthwestern.**edu > > > > > > > > > > > > > http://lists.utsouthwestern.**edu/mailman/listinfo/histonet< > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > > > > > > > > > > ______________________________**_________________ > > > > > Histonet mailing list > > > > > Histonet@lists.utsouthwestern.**edu > > > > > > > > > > > > > http://lists.utsouthwestern.**edu/mailman/listinfo/histonet< > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > > > > > > > > > > > > _______________________________________________ > > > > Histonet mailing list > > > > Histonet@lists.utsouthwestern.edu > > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Tue, 10 Sep 2013 15:47:35 +0000 From: "Weems, Joyce K." Subject: RE: [Histonet] GI biopsy slides To: "'Elizabeth Chatfield'" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" We put 3 levels on the same slide when specimen size allows. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [ mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chatfield Sent: Tuesday, September 10, 2013 11:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] GI biopsy slides Hi folks, We are re-thinking how we cut our GI biopsy slides. Currently we are cutting 3 levels with 3 sections on each slide. Is anyone out there putting all 3 levels on the same slide? We are receiving cases with a large number of samples and some our pathologists would like to see fewer slides. Thanks, Elizabeth Charlottetown, PE ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). ------------------------------ Message: 3 Date: Tue, 10 Sep 2013 08:58:36 -0700 (PDT) From: Sheila Haas Subject: Re: [Histonet] GI biopsy slides To: Elizabeth Chatfield , "histonet@lists.utsouthwestern.edu" Message-ID: <1378828716.86989.YahooMailNeo@web122002.mail.ne1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 We cut 3 levels on one slide. Each level typically has two sections across the slide. If there are multiple pieces of tissue that need to be embedded in a little larger mold, we put 3 levels on 1 slide by breaking away the excess paraffin around the sections while they're on the waterbath. Then we can then fit 3 levels on 1 slide but each level will only have 1 section. Hope this makes sense. Sheila Haas Laboratory Manager MicroPath Laboratories, Inc. ________________________________ From: Elizabeth Chatfield To: histonet@lists.utsouthwestern.edu Sent: Tuesday, September 10, 2013 11:31 AM Subject: [Histonet] GI biopsy slides Hi folks, We are re-thinking how we cut our GI biopsy slides. Currently we are cutting 3 levels with 3 sections on each slide. Is anyone out there putting all 3 levels on the same slide? We are receiving cases with a large number of samples and some our pathologists would like to see fewer slides. Thanks, Elizabeth Charlottetown, PE ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Tue, 10 Sep 2013 16:02:22 +0000 From: "Norton, Sally" Subject: RE: [Histonet] GI biopsy slides To: "'Weems, Joyce K.'" , 'Elizabeth Chatfield' , "histonet@lists.utsouthwestern.edu" Message-ID: <1357F84B33D39A46BA015A8EC6ABCBD03F3734A3@PPWEXD01B.childrens.sea.kids> Content-Type: text/plain; charset="us-ascii" Are your specimens large? We cut two levels, with two ribbons on each slide. Sally Norton Seattle children's Hospital -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [ mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Tuesday, September 10, 2013 08:48 To: 'Elizabeth Chatfield'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] GI biopsy slides We put 3 levels on the same slide when specimen size allows. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [ mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chatfield Sent: Tuesday, September 10, 2013 11:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] GI biopsy slides Hi folks, We are re-thinking how we cut our GI biopsy slides. Currently we are cutting 3 levels with 3 sections on each slide. Is anyone out there putting all 3 levels on the same slide? We are receiving cases with a large number of samples and some our pathologists would like to see fewer slides. Thanks, Elizabeth Charlottetown, PE ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 118, Issue 14 ***************************************** From joelleweaver <@t> hotmail.com Tue Sep 10 14:59:27 2013 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Tue Sep 10 14:59:31 2013 Subject: [Histonet] Unregistered HT In-Reply-To: <41D3A1AF6FEF0643BDC89E0516A6EA32D31581E1@Mail2Node2.ad.uams.edu> References: , , , <41D3A1AF6FEF0643BDC89E0516A6EA32D31581E1@Mail2Node2.ad.uams.edu> Message-ID: Well I am mostly clinical...but I think that organizations can set standards outside and beyond what CAP,CLIA etc stipulate. For the position I have now, I had to submit all my transcripts from high school up through masters in addition to proof of my ASCP certification, IHC qualification, continuing education, and professional association activity. There is a lot of gray area out there. They seem to have not had trouble getting applicants though ( and I know this varies by market), there were over 8 candidates for an HT opening, which I thought was a pretty good turn out. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: PAMarcum@uams.edu > To: talulahgosh@gmail.com; joelleweaver@hotmail.com > CC: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Unregistered HT > Date: Tue, 10 Sep 2013 13:53:26 +0000 > > Research is a different area and not controlled by CAP, CLIA and other hospital licensing or accreditation organizations. We are bound by the rules of these organizations and while I agree with you to a point. We do need minimums for training and registration by recognized licensing bodies when patient tissue is being processed for histological examination. I am sure no one thinks of this often however; there are medical legal issues with insurance we have that do not apply for research. It is also clear that registration does not mean we don't have registered people who are not as good as they should be for excellent patient care. > > I have worked in research and while I would not ever say the hiring of non-registered people is a problem for research. It is often a specialty area that requires knowing more than routine Histology. I have done plastics in research that could not ever be used in routine Histology due to the time factors and in some cases limited use with staining applications, especially IHC for some procedures. Many other areas in research require more specialized training than would be used in a routine area. I would also add some really great techs are in many phases of research. I know MT who work in Histology and are not registered as the MT BS, overrides the HT requirement for many institutions. > > Many factors must be considered for both research and routine Histology that cover far more than just hiring registered people in certain areas of the laboratory. > > Pam Marcum > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours > Sent: Tuesday, September 10, 2013 8:20 AM > To: joelle weaver > Cc: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Unregistered HT > > Do employers consider lab techs to be proficient enough? I've been doing ISH and immuno for 13 years, but I'm not certified as I do research. Maybe there aren't a lot of lab techs out there? Just wondering since you might be missing someone awesome by hiring only certified people. > > "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." > > -Chuck Palahniuk, "Haunted" > > > On Mon, Sep 9, 2013 at 6:49 PM, joelle weaver wrote: > > > All I have is a "histology assistant" description I put together. It > > is mostly clerical, instrument up keep and other duties. My employer > > does not hire uncertified techs- due to CLIA license, grossing, FISH & > > molecular duties ( high complexity). I hope to be a clinical site > > eventually, and then they will have to complete their certification > > within one year -if they are hired on. > > If you think that will help, I can send it on. > > > > > > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > > > Date: Mon, 9 Sep 2013 15:39:12 -0500 > > > From: delsuec@gmail.com > > > To: histonet@lists.utsouthwestern.edu > > > Subject: [Histonet] Unregistered HT > > > > > > Hi all, > > > > > > I'm wondering if anyone can share a job description for an > > > unregistered > > HT > > > position. I also need to know if unregistered HT's are allowed to > > > cut frozen sections. I know there is a limit to what they can do, > > > especially where IHC and special staining are concerned, but I > > > really don't know > > about > > > frozens, since it's a lot like regular microtomy, except for the H&E > > > staining of the slide. I also understand that facilities might have > > > different descriptions, but what I'm really looking for is what CAP > > allows > > > them to do. > > > > > > Thanks for your help, > > > > > > Deloris Carter, HT(ASCP) > > > SMMC > > > Shawnee, KS > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ---------------------------------------------------------------------- > Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From PAMarcum <@t> uams.edu Tue Sep 10 15:10:41 2013 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Tue Sep 10 15:10:55 2013 Subject: [Histonet] Unregistered HT In-Reply-To: References: , , , <41D3A1AF6FEF0643BDC89E0516A6EA32D31581E1@Mail2Node2.ad.uams.edu> Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA32D3158371@Mail2Node2.ad.uams.edu> I agree we have huge gray areas and not all histology schools are as good as they could be for what we are facing in Histology. I keep harping on the fact that until we are recognized as Laboratory Professionals we will stay in this limbo. The rules determining complex testing should be revisited to what is done in Histology Laboratories today and not what we did 30 or more years ago. The Clinical Laboratory is now so automated it is hard to find anyone in most areas who can even remember doing any manual testing. The Micro lab is the closest to being as manual as areas of Histology. I am in a small market and finding a registered Histologist is harder for us. I would love to have 8 to choose from and interview. Pam Marcum From: joelle weaver [mailto:joelleweaver@hotmail.com] Sent: Tuesday, September 10, 2013 2:59 PM To: Marcum, Pamela A; 'Emily Sours'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT Well I am mostly clinical...but I think that organizations can set standards outside and beyond what CAP,CLIA etc stipulate. For the position I have now, I had to submit all my transcripts from high school up through masters in addition to proof of my ASCP certification, IHC qualification, continuing education, and professional association activity. There is a lot of gray area out there. They seem to have not had trouble getting applicants though ( and I know this varies by market), there were over 8 candidates for an HT opening, which I thought was a pretty good turn out. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: PAMarcum@uams.edu > To: talulahgosh@gmail.com; joelleweaver@hotmail.com > CC: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Unregistered HT > Date: Tue, 10 Sep 2013 13:53:26 +0000 > > Research is a different area and not controlled by CAP, CLIA and other hospital licensing or accreditation organizations. We are bound by the rules of these organizations and while I agree with you to a point. We do need minimums for training and registration by recognized licensing bodies when patient tissue is being processed for histological examination. I am sure no one thinks of this often however; there are medical legal issues with insurance we have that do not apply for research. It is also clear that registration does not mean we don't have registered people who are not as good as they should be for excellent patient care. > > I have worked in research and while I would not ever say the hiring of non-registered people is a problem for research. It is often a specialty area that requires knowing more than routine Histology. I have done plastics in research that could not ever be used in routine Histology due to the time factors and in some cases limited use with staining applications, especially IHC for some procedures. Many other areas in research require more specialized training than would be used in a routine area. I would also add some really great techs are in many phases of research. I know MT who work in Histology and are not registered as the MT BS, overrides the HT requirement for many institutions. > > Many factors must be considered for both research and routine Histology that cover far more than just hiring registered people in certain areas of the laboratory. > > Pam Marcum > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours > Sent: Tuesday, September 10, 2013 8:20 AM > To: joelle weaver > Cc: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Unregistered HT > > Do employers consider lab techs to be proficient enough? I've been doing ISH and immuno for 13 years, but I'm not certified as I do research. Maybe there aren't a lot of lab techs out there? Just wondering since you might be missing someone awesome by hiring only certified people. > > "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." > > -Chuck Palahniuk, "Haunted" > > > On Mon, Sep 9, 2013 at 6:49 PM, joelle weaver >wrote: > > > All I have is a "histology assistant" description I put together. It > > is mostly clerical, instrument up keep and other duties. My employer > > does not hire uncertified techs- due to CLIA license, grossing, FISH & > > molecular duties ( high complexity). I hope to be a clinical site > > eventually, and then they will have to complete their certification > > within one year -if they are hired on. > > If you think that will help, I can send it on. > > > > > > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > > > Date: Mon, 9 Sep 2013 15:39:12 -0500 > > > From: delsuec@gmail.com > > > To: histonet@lists.utsouthwestern.edu > > > Subject: [Histonet] Unregistered HT > > > > > > Hi all, > > > > > > I'm wondering if anyone can share a job description for an > > > unregistered > > HT > > > position. I also need to know if unregistered HT's are allowed to > > > cut frozen sections. I know there is a limit to what they can do, > > > especially where IHC and special staining are concerned, but I > > > really don't know > > about > > > frozens, since it's a lot like regular microtomy, except for the H&E > > > staining of the slide. I also understand that facilities might have > > > different descriptions, but what I'm really looking for is what CAP > > allows > > > them to do. > > > > > > Thanks for your help, > > > > > > Deloris Carter, HT(ASCP) > > > SMMC > > > Shawnee, KS > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ---------------------------------------------------------------------- > Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From jaylundgren <@t> gmail.com Tue Sep 10 15:15:38 2013 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Tue Sep 10 15:15:50 2013 Subject: [Histonet] Travel Histology Technician Jobs In-Reply-To: References: <0082E20E79ED2245AB1DD78B87CFA1094912C9A4@cpd-exchange.ClinPath.local> <6DB0AEDB1C984207823DB5DFA9BBC3E0@HP2010> <761E2B5697F795489C8710BCC72141FF098EDA@ex07.net.ucsf.edu> Message-ID: Travel RNs make two times the money of traveling HTs. There is more margin for expenses. The business model is this: The agency charges the facility looking for help one hourly rate, call it X. The agency pays the traveler's wage and expenses (lodging, travel), call this Y. X-Y=Z, Z is the agency's profit. Every cent that the agency reduces Y, increases profit. Now, you can always take the lodging allowance in cash and find your own housing. Maybe this is what your nurse friend did. In fact the agency would love you to do this, as it's less work for them, and it saves them money. How, you ask? Because the housing allowance they offer you will only be 75% of what it costs to live in a given locale, unless you want to live in a slum. Another opportunity to subsidize your own expenses from your paltry, substandard wages! Go ahead, argue with the agency housing office, send screencaps from real estate websites, it won't matter. Remember EVERY PENNY THEY DON'T SPEND ON YOU GOES INTO THEIR OWN POCKETS. Also remember, these days, there is always, always, always, a desperate, unemployed, registered, JCAHO compliant histotech who would be happy to live in a dumpster for 3 months, if only to have a job. Sorry to burst anyone's bubble. But I have been traveling for a long time now, 16 years, 14 states, dozens of labs, just about every agency. Perhaps a wise person could learn from my experience. Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) On Tue, Sep 10, 2013 at 10:45 AM, jeff lowen wrote: > If they just let us HT's make up the rules, things would be a lot better, > for us anyway? > > > From: jqb7@cdc.gov > > To: lowenjeff@hotmail.com; timothy.morken@ucsfmedctr.org; > jaylundgren@gmail.com > > > CC: histonet@lists.utsouthwestern.edu; cjacobs@clinpath.com > > Subject: RE: [Histonet] Travel Histology Technician Jobs > > Date: Tue, 10 Sep 2013 15:38:13 +0000 > > > > > Or cabana boys and mojitos! > > > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of jeff lowen > > Sent: Tuesday, September 10, 2013 11:11 AM > > To: Morken, Timothy; Jay Lundgren > > Cc: histonet; Christopher Jacobs > > Subject: RE: [Histonet] Travel Histology Technician Jobs > > > > Me too? umbrella girls and champagne for all > > > > > From: Timothy.Morken@ucsfmedctr.org > > > To: jaylundgren@gmail.com; lowenjeff@hotmail.com > > > CC: histonet@lists.utsouthwestern.edu; cjacobs@clinpath.com > > > Subject: RE: [Histonet] Travel Histology Technician Jobs > > > Date: Tue, 10 Sep 2013 15:08:03 +0000 > > > > > > " It ain't all speedboats and supermodels, if that's what you had in > mind." > > > > > > Bummer, That's exactly what I had in mind....!! > > > > > > > > > Tim > > > > > > -----Original Message----- > > > From: histonet-bounces@lists.utsouthwestern.edu > > > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jay > > > Lundgren > > > Sent: Monday, September 09, 2013 5:07 PM > > > To: jeff lowen > > > Cc: histonet; Christopher Jacobs > > > Subject: Re: [Histonet] Travel Histology Technician Jobs > > > > > > I honestly wouldn't recommend it unless you are financially able > > > to undergo 6 month plus periods of unemployment. In 2008 I didn't > > > work all year, only maybe the last 3 weeks. The wages have never come > > > back to the > > > pre- 2008 levels. The largest staffing agency in the country declared > bankruptcy because of the downturn. > > > In today's economy, there is always going to be an unemployed person > who is willing to work for a couple of dollars less, and this affects the > agencies as well. Most of the HR depts I've dealt with lately, ONLY care > about cost. As long as the applicant is JCAHO compliant and registered, > they'll take the one who is 50 cents cheaper. > > > So, right now, the agencies are able to get people for > > > ridiculously low wages. I won't be as crass as to post any, but they > > > are basically asking you to subsidize your own working vacation. > > > Usually in a semi-rural, isolated, or climatically blasted location > > > (there's a reason they can't get a full time tech), living in the > > > cheapest motel in town for > > > 3 months. When you ask the agency why they put you in that particular > motel, they reply, "Because it's the cheapest place in town. (DUH)" Some > agencies are better than others, but they are all SUPER cheap. The better > ones are just honest about it. > > > I see full time jobs advertised regionally (Texas) that are offering > registered techs jobs, right out of school, $4-5 more than what the > agencies will offer you just starting out as a traveler, regardless of > previous experience. Also, travel = NO BENEFITS. Some of the agencies > pretend to have benefits, but as I said before, it's near impossible to > work with enough continuity to remain covered. > > > Also, big warning: A lot of the "agencies" aren't really agencies at > all, but someone with an 800 number and a website and some hold music. > > > It ain't all speedboats and supermodels, if that's what you had in > mind. > > > > > > Sincerely, > > > > > > Jay A. Lundgren, M.S., HTL > > > (ASCP) > > > > > > > > > On Mon, Sep 9, 2013 at 6:03 PM, jeff lowen > wrote: > > > > > > > Thanks, this is very timely as I'm looking into temp/travel HT. > > > > Any suggestions where to look, which agencies? > > > > Thanks, Jeff Lowen HT/MLT(ASCP) > > > > > > > > > Date: Mon, 9 Sep 2013 17:52:34 -0500 > > > > > From: jaylundgren@gmail.com > > > > > To: lpwenk@sbcglobal.net > > > > > Subject: Re: [Histonet] Travel Histology Technician Jobs > > > > > CC: histonet@lists.utsouthwestern.edu; CJacobs@clinpath.com > > > > > > > > > > > > > > I've been traveling for the past 16 years! Not everyone who > > > > > wanders is lost. > > > > > > > > > > Sincerely, > > > > > > > > > > Jay A. Lundgren, > > > > > M.S., HTL (ASCP) > > > > > > > > > > > > > > > > > > > > > > > > > On Mon, Sep 9, 2013 at 2:00 PM, Lee & Peggy Wenk > > > > > > > > >wrote: > > > > > > > > > > > On Sunday, Sept. 22, from 8 am - 9:30 am at the NSH Symposium in > > > > > > Providence, RI, Beth Cox, HTL/SCT(ASCP)QIHC is presenting a > > > > > > workshop on "Work and Play Across the USA - A Guide to Being a > Traveling Tech". > > > > > > http://www.histoconvention.**org/ > > > > > > > > > > > > > > > > Click on Schedule > > > > > > > > > > > > Peggy A. Wenk, HTL(ASCP)SLS > > > > > > > > > > > > -----Original Message----- From: Christopher Jacobs > > > > > > Sent: Monday, September 09, 2013 1:30 PM > > > > > > To: 'histonet@lists.**utsouthwestern.edu< > > > > histonet@lists.utsouthwestern.edu> > > > > > > > > > > ' > > > > > > Subject: [Histonet] Travel Histology Technician Jobs > > > > > > > > > > > > > > > > > > Histonetters, > > > > > > > > > > > > Does anyone out there have any information on traveling > > > > > > histology technician jobs? What is the compensation? How does > insurance work? > > > > What > > > > > > qualifications are needed? I am particularly interested in any > > > > > > personal experiences any one has had. > > > > > > > > > > > > Thanks! > > > > > > > > > > > > "CJ" Christopher P. Jacobs, HT QIHC(ASCP) Clin-Path Diagnostics, > > > > > > LLC > > > > > > > > > > > > > > > > > > > > > > > > CONFIDENTIALITY NOTICE: This message and any attachments are for > > > > > > the > > > > sole > > > > > > use of the intended recipient(s). This message is confidential > > > > > > and may > > > > also > > > > > > be privileged. If you are not the intended recipient, please > > > > > > contact > > > > the > > > > > > sender immediately, delete the contents of this message and do > > > > > > not use > > > > it > > > > > > for any purpose. > > > > > > > > > > > > ______________________________**_________________ > > > > > > Histonet mailing list > > > > > > Histonet@lists.utsouthwestern.**edu > > > > > > > > > > > > > > > > http://lists.utsouthwestern.**edu/mailman/listinfo/histonet< > > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > > > > > > > > > > > > ______________________________**_________________ > > > > > > Histonet mailing list > > > > > > Histonet@lists.utsouthwestern.**edu > > > > > > > > > > > > > > > > http://lists.utsouthwestern.**edu/mailman/listinfo/histonet< > > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > > > > > > > > > > > > > > > _______________________________________________ > > > > > Histonet mailing list > > > > > Histonet@lists.utsouthwestern.edu > > > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From talulahgosh <@t> gmail.com Tue Sep 10 15:16:25 2013 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Tue Sep 10 15:16:30 2013 Subject: [Histonet] Unregistered HT In-Reply-To: <41D3A1AF6FEF0643BDC89E0516A6EA32D3158371@Mail2Node2.ad.uams.edu> References: <41D3A1AF6FEF0643BDC89E0516A6EA32D31581E1@Mail2Node2.ad.uams.edu> <41D3A1AF6FEF0643BDC89E0516A6EA32D3158371@Mail2Node2.ad.uams.edu> Message-ID: I didn't think about the legal issues hospitals would have. That is a very good reason to certify people! It's also something I need to consider if I want to take the certification test, because while I might know antibodies and histology, legal issues are not my strong suit. Thanks!! Emily "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." -Chuck Palahniuk, "Haunted" On Tue, Sep 10, 2013 at 4:10 PM, Marcum, Pamela A wrote: > I agree we have huge gray areas and not all histology schools are as > good as they could be for what we are facing in Histology. I keep harping > on the fact that until we are recognized as Laboratory Professionals we > will stay in this limbo. The rules determining complex testing should be > revisited to what is done in Histology Laboratories today and not what we > did 30 or more years ago. The Clinical Laboratory is now so automated it > is hard to find anyone in most areas who can even remember doing any manual > testing. The Micro lab is the closest to being as manual as areas of > Histology. **** > > ** ** > > I am in a small market and finding a registered Histologist is harder for > us. I would love to have 8 to choose from and interview. **** > > ** ** > > Pam Marcum **** > > ** ** > > *From:* joelle weaver [mailto:joelleweaver@hotmail.com] > *Sent:* Tuesday, September 10, 2013 2:59 PM > *To:* Marcum, Pamela A; 'Emily Sours'; histonet@lists.utsouthwestern.edu > *Subject:* RE: [Histonet] Unregistered HT**** > > ** ** > > Well I am mostly clinical...but I think that organizations can set > standards outside and beyond what CAP,CLIA etc stipulate. For the position > I have now, I had to submit all my transcripts from high school up through > masters in addition to proof of my ASCP certification, IHC qualification, > continuing education, and professional association activity. There is a lot > of gray area out there. They seem to have not had trouble getting > applicants though ( and I know this varies by market), there were over 8 > candidates for an HT opening, which I thought was a pretty good turn out. > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > **** > > > From: PAMarcum@uams.edu > > To: talulahgosh@gmail.com; joelleweaver@hotmail.com > > CC: histonet@lists.utsouthwestern.edu > > Subject: RE: [Histonet] Unregistered HT > > Date: Tue, 10 Sep 2013 13:53:26 +0000 > > > > Research is a different area and not controlled by CAP, CLIA and other > hospital licensing or accreditation organizations. We are bound by the > rules of these organizations and while I agree with you to a point. We do > need minimums for training and registration by recognized licensing bodies > when patient tissue is being processed for histological examination. I am > sure no one thinks of this often however; there are medical legal issues > with insurance we have that do not apply for research. It is also clear > that registration does not mean we don't have registered people who are not > as good as they should be for excellent patient care. > > > > I have worked in research and while I would not ever say the hiring of > non-registered people is a problem for research. It is often a specialty > area that requires knowing more than routine Histology. I have done > plastics in research that could not ever be used in routine Histology due > to the time factors and in some cases limited use with staining > applications, especially IHC for some procedures. Many other areas in > research require more specialized training than would be used in a routine > area. I would also add some really great techs are in many phases of > research. I know MT who work in Histology and are not registered as the MT > BS, overrides the HT requirement for many institutions. > > > > Many factors must be considered for both research and routine Histology > that cover far more than just hiring registered people in certain areas of > the laboratory. > > > > Pam Marcum > > > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily > Sours > > Sent: Tuesday, September 10, 2013 8:20 AM > > To: joelle weaver > > Cc: histonet@lists.utsouthwestern.edu > > Subject: Re: [Histonet] Unregistered HT > > > > Do employers consider lab techs to be proficient enough? I've been doing > ISH and immuno for 13 years, but I'm not certified as I do research. Maybe > there aren't a lot of lab techs out there? Just wondering since you might > be missing someone awesome by hiring only certified people. > > > > "By bitching and bitching and bitching, they could exhaust the drama of > their own horror stories. Grow bored. Only then could they accept a new > story for their lives. Move forward." > > > > -Chuck Palahniuk, "Haunted" > > > > > > On Mon, Sep 9, 2013 at 6:49 PM, joelle weaver >wrote: > > > > > All I have is a "histology assistant" description I put together. It > > > is mostly clerical, instrument up keep and other duties. My employer > > > does not hire uncertified techs- due to CLIA license, grossing, FISH & > > > molecular duties ( high complexity). I hope to be a clinical site > > > eventually, and then they will have to complete their certification > > > within one year -if they are hired on. > > > If you think that will help, I can send it on. > > > > > > > > > > > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > > > > > Date: Mon, 9 Sep 2013 15:39:12 -0500 > > > > From: delsuec@gmail.com > > > > To: histonet@lists.utsouthwestern.edu > > > > Subject: [Histonet] Unregistered HT > > > > > > > > Hi all, > > > > > > > > I'm wondering if anyone can share a job description for an > > > > unregistered > > > HT > > > > position. I also need to know if unregistered HT's are allowed to > > > > cut frozen sections. I know there is a limit to what they can do, > > > > especially where IHC and special staining are concerned, but I > > > > really don't know > > > about > > > > frozens, since it's a lot like regular microtomy, except for the H&E > > > > staining of the slide. I also understand that facilities might have > > > > different descriptions, but what I'm really looking for is what CAP > > > allows > > > > them to do. > > > > > > > > Thanks for your help, > > > > > > > > Deloris Carter, HT(ASCP) > > > > SMMC > > > > Shawnee, KS > > > > _______________________________________________ > > > > Histonet mailing list > > > > Histonet@lists.utsouthwestern.edu > > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ---------------------------------------------------------------------- > > Confidentiality Notice: This e-mail message, including any attachments, > is for the sole use of the intended recipient(s) and may contain > confidential and privileged information. Any unauthorized review, use, > disclosure or distribution is prohibited. If you are not the intended > recipient, please contact the sender by reply e-mail and destroy all copies > of the original message.**** > From Richard.Cartun <@t> hhchealth.org Tue Sep 10 17:19:25 2013 From: Richard.Cartun <@t> hhchealth.org (Cartun, Richard) Date: Tue Sep 10 17:19:31 2013 Subject: [Histonet] RE: VIP antibody In-Reply-To: <7B81E779-146C-4D3D-BF09-2D8BDC202DC7@med.lu.se> References: <7B81E779-146C-4D3D-BF09-2D8BDC202DC7@med.lu.se> Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E0188279A@HHCEXCHMB05.hhcsystem.org> We obtained an excellent rabbit anti-VIP antibody from Biogenesis in Sandown, NH back in 1995. I just called their telephone number (listed on the product data sheet) and it has been disconnected. I think the parent company may be located in the UK. Maybe someone on Histonet knows more about this company their status. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax richard.cartun@hhchealth.org ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Leila Etemadi [leila.etemadi@med.lu.se] Sent: Monday, September 09, 2013 1:27 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] VIP antibody Hello every body, I wounded if any of you is working with VIP antibody ( rabbit)?, Any advice for selecting right supplier will be appreciated. The best of this antibody used to be presented by Eurodiagnostica, which unfortunately they are not presenting it any more for IHC project ( they just sell the kit now a days). Santa cruz, milipore?.?!?, Any suggestion?!? Many thanks, Leila :-) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From JMacDonald <@t> mtsac.edu Tue Sep 10 18:32:29 2013 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Tue Sep 10 18:29:53 2013 Subject: [Histonet] Unregistered HT In-Reply-To: <41D3A1AF6FEF0643BDC89E0516A6EA32D3158371@Mail2Node2.ad.uams.edu> References: , , , <41D3A1AF6FEF0643BDC89E0516A6EA32D31581E1@Mail2Node2.ad.uams.edu> <41D3A1AF6FEF0643BDC89E0516A6EA32D3158371@Mail2Node2.ad.uams.edu> Message-ID: As long as we do not need certification, licensure and minium education requirements we will not be recognized as Laboratory Professionals. From: "Marcum, Pamela A" To: "'joelle weaver'" , "'Emily Sours'" , "histonet@lists.utsouthwestern.edu" Date: 09/10/2013 01:12 PM Subject: RE: [Histonet] Unregistered HT Sent by: histonet-bounces@lists.utsouthwestern.edu I agree we have huge gray areas and not all histology schools are as good as they could be for what we are facing in Histology. I keep harping on the fact that until we are recognized as Laboratory Professionals we will stay in this limbo. The rules determining complex testing should be revisited to what is done in Histology Laboratories today and not what we did 30 or more years ago. The Clinical Laboratory is now so automated it is hard to find anyone in most areas who can even remember doing any manual testing. The Micro lab is the closest to being as manual as areas of Histology. I am in a small market and finding a registered Histologist is harder for us. I would love to have 8 to choose from and interview. Pam Marcum From: joelle weaver [mailto:joelleweaver@hotmail.com] Sent: Tuesday, September 10, 2013 2:59 PM To: Marcum, Pamela A; 'Emily Sours'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT Well I am mostly clinical...but I think that organizations can set standards outside and beyond what CAP,CLIA etc stipulate. For the position I have now, I had to submit all my transcripts from high school up through masters in addition to proof of my ASCP certification, IHC qualification, continuing education, and professional association activity. There is a lot of gray area out there. They seem to have not had trouble getting applicants though ( and I know this varies by market), there were over 8 candidates for an HT opening, which I thought was a pretty good turn out. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: PAMarcum@uams.edu > To: talulahgosh@gmail.com; joelleweaver@hotmail.com > CC: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Unregistered HT > Date: Tue, 10 Sep 2013 13:53:26 +0000 > > Research is a different area and not controlled by CAP, CLIA and other hospital licensing or accreditation organizations. We are bound by the rules of these organizations and while I agree with you to a point. We do need minimums for training and registration by recognized licensing bodies when patient tissue is being processed for histological examination. I am sure no one thinks of this often however; there are medical legal issues with insurance we have that do not apply for research. It is also clear that registration does not mean we don't have registered people who are not as good as they should be for excellent patient care. > > I have worked in research and while I would not ever say the hiring of non-registered people is a problem for research. It is often a specialty area that requires knowing more than routine Histology. I have done plastics in research that could not ever be used in routine Histology due to the time factors and in some cases limited use with staining applications, especially IHC for some procedures. Many other areas in research require more specialized training than would be used in a routine area. I would also add some really great techs are in many phases of research. I know MT who work in Histology and are not registered as the MT BS, overrides the HT requirement for many institutions. > > Many factors must be considered for both research and routine Histology that cover far more than just hiring registered people in certain areas of the laboratory. > > Pam Marcum > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu< mailto:histonet-bounces@lists.utsouthwestern.edu> [ mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours > Sent: Tuesday, September 10, 2013 8:20 AM > To: joelle weaver > Cc: histonet@lists.utsouthwestern.edu< mailto:histonet@lists.utsouthwestern.edu> > Subject: Re: [Histonet] Unregistered HT > > Do employers consider lab techs to be proficient enough? I've been doing ISH and immuno for 13 years, but I'm not certified as I do research. Maybe there aren't a lot of lab techs out there? Just wondering since you might be missing someone awesome by hiring only certified people. > > "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." > > -Chuck Palahniuk, "Haunted" > > > On Mon, Sep 9, 2013 at 6:49 PM, joelle weaver >wrote: > > > All I have is a "histology assistant" description I put together. It > > is mostly clerical, instrument up keep and other duties. My employer > > does not hire uncertified techs- due to CLIA license, grossing, FISH & > > molecular duties ( high complexity). I hope to be a clinical site > > eventually, and then they will have to complete their certification > > within one year -if they are hired on. > > If you think that will help, I can send it on. > > > > > > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > > > Date: Mon, 9 Sep 2013 15:39:12 -0500 > > > From: delsuec@gmail.com > > > To: histonet@lists.utsouthwestern.edu< mailto:histonet@lists.utsouthwestern.edu> > > > Subject: [Histonet] Unregistered HT > > > > > > Hi all, > > > > > > I'm wondering if anyone can share a job description for an > > > unregistered > > HT > > > position. I also need to know if unregistered HT's are allowed to > > > cut frozen sections. I know there is a limit to what they can do, > > > especially where IHC and special staining are concerned, but I > > > really don't know > > about > > > frozens, since it's a lot like regular microtomy, except for the H&E > > > staining of the slide. I also understand that facilities might have > > > different descriptions, but what I'm really looking for is what CAP > > allows > > > them to do. > > > > > > Thanks for your help, > > > > > > Deloris Carter, HT(ASCP) > > > SMMC > > > Shawnee, KS > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu< mailto:Histonet@lists.utsouthwestern.edu> > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu< mailto:Histonet@lists.utsouthwestern.edu> > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu< mailto:Histonet@lists.utsouthwestern.edu> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ---------------------------------------------------------------------- > Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cforster <@t> umn.edu Tue Sep 10 18:35:45 2013 From: cforster <@t> umn.edu (Colleen Forster) Date: Tue Sep 10 18:35:50 2013 Subject: [Histonet] Unregistered HT In-Reply-To: References: , , , <41D3A1AF6FEF0643BDC89E0516A6EA32D31581E1@Mail2Node2.ad.uams.edu> <41D3A1AF6FEF0643BDC89E0516A6EA32D3158371@Mail2Node2.ad.uams.edu> Message-ID: <522FACD1.2000506@umn.edu> Exactly Jennifer! C On 9/10/2013 6:32 PM, Jennifer MacDonald wrote: > As long as we do not need certification, licensure and minium education > requirements we will not be recognized as Laboratory Professionals. > > > > From: "Marcum, Pamela A" > To: "'joelle weaver'" , "'Emily Sours'" > , "histonet@lists.utsouthwestern.edu" > > Date: 09/10/2013 01:12 PM > Subject: RE: [Histonet] Unregistered HT > Sent by: histonet-bounces@lists.utsouthwestern.edu > > > > I agree we have huge gray areas and not all histology schools are as good > as they could be for what we are facing in Histology. I keep harping on > the fact that until we are recognized as Laboratory Professionals we will > stay in this limbo. The rules determining complex testing should be > revisited to what is done in Histology Laboratories today and not what we > did 30 or more years ago. The Clinical Laboratory is now so automated it > is hard to find anyone in most areas who can even remember doing any > manual testing. The Micro lab is the closest to being as manual as areas > of Histology. > > I am in a small market and finding a registered Histologist is harder for > us. I would love to have 8 to choose from and interview. > > Pam Marcum > > From: joelle weaver [mailto:joelleweaver@hotmail.com] > Sent: Tuesday, September 10, 2013 2:59 PM > To: Marcum, Pamela A; 'Emily Sours'; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Unregistered HT > > Well I am mostly clinical...but I think that organizations can set > standards outside and beyond what CAP,CLIA etc stipulate. For the position > I have now, I had to submit all my transcripts from high school up through > masters in addition to proof of my ASCP certification, IHC qualification, > continuing education, and professional association activity. There is a > lot of gray area out there. They seem to have not had trouble getting > applicants though ( and I know this varies by market), there were over 8 > candidates for an HT opening, which I thought was a pretty good turn out. > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > >> From: PAMarcum@uams.edu >> To: talulahgosh@gmail.com; joelleweaver@hotmail.com >> CC: histonet@lists.utsouthwestern.edu >> Subject: RE: [Histonet] Unregistered HT >> Date: Tue, 10 Sep 2013 13:53:26 +0000 >> >> Research is a different area and not controlled by CAP, CLIA and other > hospital licensing or accreditation organizations. We are bound by the > rules of these organizations and while I agree with you to a point. We do > need minimums for training and registration by recognized licensing bodies > when patient tissue is being processed for histological examination. I am > sure no one thinks of this often however; there are medical legal issues > with insurance we have that do not apply for research. It is also clear > that registration does not mean we don't have registered people who are > not as good as they should be for excellent patient care. >> I have worked in research and while I would not ever say the hiring of > non-registered people is a problem for research. It is often a specialty > area that requires knowing more than routine Histology. I have done > plastics in research that could not ever be used in routine Histology due > to the time factors and in some cases limited use with staining > applications, especially IHC for some procedures. Many other areas in > research require more specialized training than would be used in a routine > area. I would also add some really great techs are in many phases of > research. I know MT who work in Histology and are not registered as the MT > BS, overrides the HT requirement for many institutions. >> Many factors must be considered for both research and routine Histology > that cover far more than just hiring registered people in certain areas of > the laboratory. >> Pam Marcum >> >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu< > mailto:histonet-bounces@lists.utsouthwestern.edu> [ > mailto:histonet-bounces@lists.utsouthwestern.edu] mailto:histonet-bounces@lists.utsouthwestern.edu]> On Behalf Of Emily > Sours >> Sent: Tuesday, September 10, 2013 8:20 AM >> To: joelle weaver >> Cc: histonet@lists.utsouthwestern.edu< > mailto:histonet@lists.utsouthwestern.edu> >> Subject: Re: [Histonet] Unregistered HT >> >> Do employers consider lab techs to be proficient enough? I've been doing > ISH and immuno for 13 years, but I'm not certified as I do research. Maybe > there aren't a lot of lab techs out there? Just wondering since you might > be missing someone awesome by hiring only certified people. >> "By bitching and bitching and bitching, they could exhaust the drama of > their own horror stories. Grow bored. Only then could they accept a new > story for their lives. Move forward." >> -Chuck Palahniuk, "Haunted" >> >> >> On Mon, Sep 9, 2013 at 6:49 PM, joelle weaver mailto:joelleweaver@hotmail.com>>wrote: >>> All I have is a "histology assistant" description I put together. It >>> is mostly clerical, instrument up keep and other duties. My employer >>> does not hire uncertified techs- due to CLIA license, grossing, FISH & >>> molecular duties ( high complexity). I hope to be a clinical site >>> eventually, and then they will have to complete their certification >>> within one year -if they are hired on. >>> If you think that will help, I can send it on. >>> >>> >>> >>> >>> Joelle Weaver MAOM, HTL (ASCP) QIHC >>> >>>> Date: Mon, 9 Sep 2013 15:39:12 -0500 >>>> From: delsuec@gmail.com >>>> To: histonet@lists.utsouthwestern.edu< > mailto:histonet@lists.utsouthwestern.edu> >>>> Subject: [Histonet] Unregistered HT >>>> >>>> Hi all, >>>> >>>> I'm wondering if anyone can share a job description for an >>>> unregistered >>> HT >>>> position. I also need to know if unregistered HT's are allowed to >>>> cut frozen sections. I know there is a limit to what they can do, >>>> especially where IHC and special staining are concerned, but I >>>> really don't know >>> about >>>> frozens, since it's a lot like regular microtomy, except for the H&E >>>> staining of the slide. I also understand that facilities might have >>>> different descriptions, but what I'm really looking for is what CAP >>> allows >>>> them to do. >>>> >>>> Thanks for your help, >>>> >>>> Deloris Carter, HT(ASCP) >>>> SMMC >>>> Shawnee, KS >>>> _______________________________________________ >>>> Histonet mailing list >>>> Histonet@lists.utsouthwestern.edu< > mailto:Histonet@lists.utsouthwestern.edu> >>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu< > mailto:Histonet@lists.utsouthwestern.edu> >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu< > mailto:Histonet@lists.utsouthwestern.edu> >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> ---------------------------------------------------------------------- >> Confidentiality Notice: This e-mail message, including any attachments, > is for the sole use of the intended recipient(s) and may contain > confidential and privileged information. Any unauthorized review, use, > disclosure or distribution is prohibited. If you are not the intended > recipient, please contact the sender by reply e-mail and destroy all > copies of the original message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Tony_Reilly <@t> health.qld.gov.au Tue Sep 10 18:48:01 2013 From: Tony_Reilly <@t> health.qld.gov.au (Tony Reilly) Date: Tue Sep 10 18:48:22 2013 Subject: [Histonet] RE: VIP antibody In-Reply-To: <9215BD4B0BA1B44D962A71C758B68D2E0188279A@HHCEXCHMB05.hhcsystem.org> References: <7B81E779-146C-4D3D-BF09-2D8BDC202DC7@med.lu.se> <9215BD4B0BA1B44D962A71C758B68D2E0188279A@HHCEXCHMB05.hhcsystem.org> Message-ID: <52303C53.411C.0039.0@health.qld.gov.au> Hi Richard Biogenesis was acquired in 2005 by a company in Martinsried Germany called Morphosys . I do not know much about them other than their core business is Antibody production and that they also acquired Serotec in 2006. regards Tony Tony Reilly B.App.Sc. , M.Sc. Chief Scientist, Anatomical Pathology Pathology Queensland-PA Laboratory ________________________________________________ Health Services Support Agency | Department of Health Level 1, Building 15,Princess Alexandra Hospital Ipswich Road,WOOLLOONGABBA Qld4102 Ph: 07 3176 2412 Mob: 0402 139411 Fax: 07 3176 2930 Email: tony_reilly@health.qld.gov.au Web: www.health.qld.gov.au/qhcss/ >>> "Cartun, Richard" 9/11/2013 8:19 am >>> We obtained an excellent rabbit anti-VIP antibody from Biogenesis in Sandown, NH back in 1995. I just called their telephone number (listed on the product data sheet) and it has been disconnected. I think the parent company may be located in the UK. Maybe someone on Histonet knows more about this company their status. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax richard.cartun@hhchealth.org ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Leila Etemadi [leila.etemadi@med.lu.se] Sent: Monday, September 09, 2013 1:27 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] VIP antibody Hello every body, I wounded if any of you is working with VIP antibody ( rabbit)?, Any advice for selecting right supplier will be appreciated. The best of this antibody used to be presented by Eurodiagnostica, which unfortunately they are not presenting it any more for IHC project ( they just sell the kit now a days). Santa cruz, milipore?.?!?, Any suggestion?!? Many thanks, Leila :-) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ********************************************************************************** From drbugge <@t> gmail.com Tue Sep 10 19:52:44 2013 From: drbugge <@t> gmail.com (Dawn Bugge) Date: Tue Sep 10 19:52:52 2013 Subject: [Histonet] APEasy software Message-ID: <-7348212797568367230@unknownmsgid> Hi Histoworld. We are currently looking into pathology software and I was wondering if anyone had suggestions and also wondering if anyone has used or is using APEasy software. Sent from my iPhone From jon2038433 <@t> maricopa.edu Wed Sep 11 02:13:34 2013 From: jon2038433 <@t> maricopa.edu (Jon Hannasch) Date: Wed Sep 11 02:13:42 2013 Subject: [Histonet] CLIA requirements In-Reply-To: <522f7e83.25103c0a.1a74.1b1eSMTPIN_ADDED_MISSING@mx.google.com> References: <522f7e83.25103c0a.1a74.1b1eSMTPIN_ADDED_MISSING@mx.google.com> Message-ID: <308E88B8-3B92-4356-AB35-017DE62CEFB4@maricopa.edu> Hello, I have two questions. 1.) is there a CLIA email newsletter I can sign up for? And 2.) Does CLIA require a laboratory to have at least 1 certified Histotechnician? I work in a small urology histology lab where there are 2 histotechs (1 certified and 1 uncertified.) This lab is located in AZ. One of the histotechs went to school to be a histotech but never got certified and the other went to school and is certified (HT). The histotechs do the accessioning/grossing and general histotech duties. Because our lab is CLIA certified and soon to be CAP certified I was wondering if it was necessary to have at least 1 certified tech working there or if we can get by with just the uncertified tech who has been doing the job for about 10 years but only went to college and is uncertified. Thanks in advanced for the information! From epchatfield <@t> ihis.org Wed Sep 11 05:04:30 2013 From: epchatfield <@t> ihis.org (Elizabeth Chatfield) Date: Wed Sep 11 05:04:50 2013 Subject: [Histonet] GI biopsy slides In-Reply-To: <3AD061FE740D464FAC7BF6B5CFB75707A8A5A95E@smcmail02.somerset-healthcare.com> References: <522F1138020000BC0005ECF4@gwia-out.peigov> <3AD061FE740D464FAC7BF6B5CFB75707A8A5A95E@smcmail02.somerset-healthcare.com> Message-ID: <523015FE020000BC0005ED97@gwia-out.peigov> Thanks for the input everyone! I can let all involved that other labs have made the same change (such a dirty word :0) Elizabeth >>> "Rathborne, Toni" 9/10/2013 2:50 PM >>> We've been doing this for years. You'd be surprised with the overall savings (slides, frequency of changing stains, storage), and also TAT since you'll be able to put through more cases on your stainer. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chatfield Sent: Tuesday, September 10, 2013 11:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] GI biopsy slides Hi folks, We are re-thinking how we cut our GI biopsy slides. Currently we are cutting 3 levels with 3 sections on each slide. Is anyone out there putting all 3 levels on the same slide? We are receiving cases with a large number of samples and some our pathologists would like to see fewer slides. Thanks, Elizabeth Charlottetown, PE ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. 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From Pat.Patterson <@t> propath.com Wed Sep 11 05:39:51 2013 From: Pat.Patterson <@t> propath.com (Pat Patterson) Date: Wed Sep 11 05:39:56 2013 Subject: [Histonet] Decloaking Chamber Message-ID: <82C7248978CB50469FD6BA68EBBEFE670AA13522@exchange.propathlab.com> Does anyone have a used BioCare Decloaking Chamber (early model - that is still functioning) that they are interested is selling? Please contact me directly at the phone/email below. Thanks! Pat Patterson, HTL(ASCP) Supervisor, Immunohistochemistry ProPath 1355 River Bend Drive Dallas, TX 75247 214-237-1700 x 2027 214-237-1730 fax pat.patterson@propath.com To learn more about ProPath, please visit http://www.ProPath.com Don't miss out! Follow ProPath(R) on Facebook, Twitter, YouTube and LinkedIn! This electronic message is intended to be for the use only of the named recipient and may contain information that is confidential or privileged. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this message in error or are not the named recipient, please notify us immediately by contacting the sender at the electronic mail address noted above, and delete and destroy all copies of this message. From claymilks <@t> hotmail.com Thu Sep 12 07:38:10 2013 From: claymilks <@t> hotmail.com (clay milks) Date: Wed Sep 11 07:38:42 2013 Subject: [Histonet] (no subject) Message-ID: I need a little help on a Patient?s rights question. It is my understanding that when a patient has a procedure, the patient has the right to request that those specimens be examined by a laboratory of their choosing. i.e. EGD, colonoscopy, etc I am in Arkansas. My father had a procedure yesterday at a local hospital. I manage a pathology laboratory that specializes in the type of tissue that the procedure procured. When it was requested that the tissue be sent to my laboratory, the hospital staff refused to fulfill the request. We asked multiple times for a release form so my father could take his tissue with him. The administration employee we spoke to said there was no such thing and that patients were not allowed to take their specimens. One employee going so far as stating that if we wanted the tissue sent somewhere aside from their contracted laboratory, that the procedure would be canceled and my father would have to go somewhere else. My father, not wanting to cause a fuss, let the issue go. Questions: Is it legal for a hospital to require that tissue specimens be sent to a lab they are contracted with? Are there any other actions we could have taken to make our requests be honored? This is not meant to insult the hospital, but to give an explanation of the situation for context in answering the questions. From christiegowan <@t> msn.com Wed Sep 11 07:50:43 2013 From: christiegowan <@t> msn.com (CHRISTIE GOWAN) Date: Wed Sep 11 07:50:48 2013 Subject: [Histonet] (no subject) In-Reply-To: References: Message-ID: Hi Clay, Most hospitals have a tissue committee that makes decisions about surgical specimens. These decisions become hospital policy. Most hospitals state that once a specimen is removed it essentially becomes the property of the hospital. Release of specimens is usually after it has been accessioned and grossed in for pathology reveiw. There is usually a form for that type of release. The hospital would be at risk if they let the specimen leave without following hospital policy. I am not saying it is right or wrong but I can see the logic in it. Once the specimen has been processed through the hospitals pathology department then blocks and slides can be requested for 2nd opinions. Good luck in your search for answers. Christie Gowan To: histonet@lists.utsouthwestern.edu Date: Thu, 12 Sep 2013 12:38:10 +0000 From: claymilks@hotmail.com Subject: [Histonet] (no subject) I need a little help on a Patient?s rights question. It is my understanding that when a patient has a procedure, the patient has the right to request that those specimens be examined by a laboratory of their choosing. i.e. EGD, colonoscopy, etc I am in Arkansas. My father had a procedure yesterday at a local hospital. I manage a pathology laboratory that specializes in the type of tissue that the procedure procured. When it was requested that the tissue be sent to my laboratory, the hospital staff refused to fulfill the request. We asked multiple times for a release form so my father could take his tissue with him. The administration employee we spoke to said there was no such thing and that patients were not allowed to take their specimens. One employee going so far as stating that if we wanted the tissue sent somewhere aside from their contracted laboratory, that the procedure would be canceled and my father would have to go somewhere else. My father, not wanting to cause a fuss, let the issue go. Questions: Is it legal for a hospital to require that tissue specimens be sent to a lab they are contracted with? Are there any other actions we could have taken to make our requests be honored? This is not meant to insult the hospital, but to give an explanation of the situation for context in answering the questions. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Joyce.Weems <@t> emoryhealthcare.org Wed Sep 11 08:07:56 2013 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Wed Sep 11 08:08:04 2013 Subject: [Histonet] Unregistered HT In-Reply-To: References: , , , <41D3A1AF6FEF0643BDC89E0516A6EA32D31581E1@Mail2Node2.ad.uams.edu> <41D3A1AF6FEF0643BDC89E0516A6EA32D3158371@Mail2Node2.ad.uams.edu> Message-ID: And the reason so many have been fighting for this for years. If a lab were looking for a Medical Technologist there would be no question. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Tuesday, September 10, 2013 7:32 PM To: Marcum, Pamela A Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT As long as we do not need certification, licensure and minium education requirements we will not be recognized as Laboratory Professionals. From: "Marcum, Pamela A" To: "'joelle weaver'" , "'Emily Sours'" , "histonet@lists.utsouthwestern.edu" Date: 09/10/2013 01:12 PM Subject: RE: [Histonet] Unregistered HT Sent by: histonet-bounces@lists.utsouthwestern.edu I agree we have huge gray areas and not all histology schools are as good as they could be for what we are facing in Histology. I keep harping on the fact that until we are recognized as Laboratory Professionals we will stay in this limbo. The rules determining complex testing should be revisited to what is done in Histology Laboratories today and not what we did 30 or more years ago. The Clinical Laboratory is now so automated it is hard to find anyone in most areas who can even remember doing any manual testing. The Micro lab is the closest to being as manual as areas of Histology. I am in a small market and finding a registered Histologist is harder for us. I would love to have 8 to choose from and interview. Pam Marcum From: joelle weaver [mailto:joelleweaver@hotmail.com] Sent: Tuesday, September 10, 2013 2:59 PM To: Marcum, Pamela A; 'Emily Sours'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT Well I am mostly clinical...but I think that organizations can set standards outside and beyond what CAP,CLIA etc stipulate. For the position I have now, I had to submit all my transcripts from high school up through masters in addition to proof of my ASCP certification, IHC qualification, continuing education, and professional association activity. There is a lot of gray area out there. They seem to have not had trouble getting applicants though ( and I know this varies by market), there were over 8 candidates for an HT opening, which I thought was a pretty good turn out. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: PAMarcum@uams.edu > To: talulahgosh@gmail.com; joelleweaver@hotmail.com > CC: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Unregistered HT > Date: Tue, 10 Sep 2013 13:53:26 +0000 > > Research is a different area and not controlled by CAP, CLIA and other hospital licensing or accreditation organizations. We are bound by the rules of these organizations and while I agree with you to a point. We do need minimums for training and registration by recognized licensing bodies when patient tissue is being processed for histological examination. I am sure no one thinks of this often however; there are medical legal issues with insurance we have that do not apply for research. It is also clear that registration does not mean we don't have registered people who are not as good as they should be for excellent patient care. > > I have worked in research and while I would not ever say the hiring of non-registered people is a problem for research. It is often a specialty area that requires knowing more than routine Histology. I have done plastics in research that could not ever be used in routine Histology due to the time factors and in some cases limited use with staining applications, especially IHC for some procedures. Many other areas in research require more specialized training than would be used in a routine area. I would also add some really great techs are in many phases of research. I know MT who work in Histology and are not registered as the MT BS, overrides the HT requirement for many institutions. > > Many factors must be considered for both research and routine Histology that cover far more than just hiring registered people in certain areas of the laboratory. > > Pam Marcum > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu< mailto:histonet-bounces@lists.utsouthwestern.edu> [ mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours > Sent: Tuesday, September 10, 2013 8:20 AM > To: joelle weaver > Cc: histonet@lists.utsouthwestern.edu< mailto:histonet@lists.utsouthwestern.edu> > Subject: Re: [Histonet] Unregistered HT > > Do employers consider lab techs to be proficient enough? I've been doing ISH and immuno for 13 years, but I'm not certified as I do research. Maybe there aren't a lot of lab techs out there? Just wondering since you might be missing someone awesome by hiring only certified people. > > "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." > > -Chuck Palahniuk, "Haunted" > > > On Mon, Sep 9, 2013 at 6:49 PM, joelle weaver >wrote: > > > All I have is a "histology assistant" description I put together. It > > is mostly clerical, instrument up keep and other duties. My employer > > does not hire uncertified techs- due to CLIA license, grossing, FISH & > > molecular duties ( high complexity). I hope to be a clinical site > > eventually, and then they will have to complete their certification > > within one year -if they are hired on. > > If you think that will help, I can send it on. > > > > > > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > > > Date: Mon, 9 Sep 2013 15:39:12 -0500 > > > From: delsuec@gmail.com > > > To: histonet@lists.utsouthwestern.edu< mailto:histonet@lists.utsouthwestern.edu> > > > Subject: [Histonet] Unregistered HT > > > > > > Hi all, > > > > > > I'm wondering if anyone can share a job description for an > > > unregistered > > HT > > > position. I also need to know if unregistered HT's are allowed to > > > cut frozen sections. I know there is a limit to what they can do, > > > especially where IHC and special staining are concerned, but I > > > really don't know > > about > > > frozens, since it's a lot like regular microtomy, except for the H&E > > > staining of the slide. I also understand that facilities might have > > > different descriptions, but what I'm really looking for is what CAP > > allows > > > them to do. > > > > > > Thanks for your help, > > > > > > Deloris Carter, HT(ASCP) > > > SMMC > > > Shawnee, KS > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu< mailto:Histonet@lists.utsouthwestern.edu> > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu< mailto:Histonet@lists.utsouthwestern.edu> > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu< mailto:Histonet@lists.utsouthwestern.edu> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ---------------------------------------------------------------------- > Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From PAMarcum <@t> uams.edu Wed Sep 11 08:18:29 2013 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Wed Sep 11 08:18:36 2013 Subject: [Histonet] Unregistered HT In-Reply-To: References: , , , <41D3A1AF6FEF0643BDC89E0516A6EA32D31581E1@Mail2Node2.ad.uams.edu> <41D3A1AF6FEF0643BDC89E0516A6EA32D3158371@Mail2Node2.ad.uams.edu> Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA32D31584FA@Mail2Node2.ad.uams.edu> We have heard for years that NSH is working on changing the status to Laboratory Professionals and so far as I have heard nothing happening to make the change. A large part of this is ASCP/all organizations inspecting/licensing are still looking at us as non-skilled labor that requires only OJT training or an AA/AS degree at best. We have a few one year schools and some online however; not enough to cover the need for HTs and HTLs. We need ASCP/CAP/CLIA/all organizations that inspect us and tell us how to run the laboratories to actually understand we have changed in the last 25 years and no longer just cut and do H&Es. I am not sure where to go as I have been bringing this up for years and see no movement. Take the test to be an inspector for CAP and you will see the problem clearly. It is 98% Clinical Laboratory and maybe 2% Anatomic Pathology. If you don't know Clinical you will have a problem passing the test without help. Pam Marcum -----Original Message----- From: Weems, Joyce K. [mailto:Joyce.Weems@emoryhealthcare.org] Sent: Wednesday, September 11, 2013 8:08 AM To: 'Jennifer MacDonald'; Marcum, Pamela A Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT And the reason so many have been fighting for this for years. If a lab were looking for a Medical Technologist there would be no question. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Tuesday, September 10, 2013 7:32 PM To: Marcum, Pamela A Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT As long as we do not need certification, licensure and minium education requirements we will not be recognized as Laboratory Professionals. From: "Marcum, Pamela A" To: "'joelle weaver'" , "'Emily Sours'" , "histonet@lists.utsouthwestern.edu" Date: 09/10/2013 01:12 PM Subject: RE: [Histonet] Unregistered HT Sent by: histonet-bounces@lists.utsouthwestern.edu I agree we have huge gray areas and not all histology schools are as good as they could be for what we are facing in Histology. I keep harping on the fact that until we are recognized as Laboratory Professionals we will stay in this limbo. The rules determining complex testing should be revisited to what is done in Histology Laboratories today and not what we did 30 or more years ago. The Clinical Laboratory is now so automated it is hard to find anyone in most areas who can even remember doing any manual testing. The Micro lab is the closest to being as manual as areas of Histology. I am in a small market and finding a registered Histologist is harder for us. I would love to have 8 to choose from and interview. Pam Marcum From: joelle weaver [mailto:joelleweaver@hotmail.com] Sent: Tuesday, September 10, 2013 2:59 PM To: Marcum, Pamela A; 'Emily Sours'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT Well I am mostly clinical...but I think that organizations can set standards outside and beyond what CAP,CLIA etc stipulate. For the position I have now, I had to submit all my transcripts from high school up through masters in addition to proof of my ASCP certification, IHC qualification, continuing education, and professional association activity. There is a lot of gray area out there. They seem to have not had trouble getting applicants though ( and I know this varies by market), there were over 8 candidates for an HT opening, which I thought was a pretty good turn out. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: PAMarcum@uams.edu > To: talulahgosh@gmail.com; joelleweaver@hotmail.com > CC: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Unregistered HT > Date: Tue, 10 Sep 2013 13:53:26 +0000 > > Research is a different area and not controlled by CAP, CLIA and other hospital licensing or accreditation organizations. We are bound by the rules of these organizations and while I agree with you to a point. We do need minimums for training and registration by recognized licensing bodies when patient tissue is being processed for histological examination. I am sure no one thinks of this often however; there are medical legal issues with insurance we have that do not apply for research. It is also clear that registration does not mean we don't have registered people who are not as good as they should be for excellent patient care. > > I have worked in research and while I would not ever say the hiring of non-registered people is a problem for research. It is often a specialty area that requires knowing more than routine Histology. I have done plastics in research that could not ever be used in routine Histology due to the time factors and in some cases limited use with staining applications, especially IHC for some procedures. Many other areas in research require more specialized training than would be used in a routine area. I would also add some really great techs are in many phases of research. I know MT who work in Histology and are not registered as the MT BS, overrides the HT requirement for many institutions. > > Many factors must be considered for both research and routine > Histology that cover far more than just hiring registered people in certain areas of the laboratory. > > Pam Marcum > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu< mailto:histonet-bounces@lists.utsouthwestern.edu> [ mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours > Sent: Tuesday, September 10, 2013 8:20 AM > To: joelle weaver > Cc: histonet@lists.utsouthwestern.edu< mailto:histonet@lists.utsouthwestern.edu> > Subject: Re: [Histonet] Unregistered HT > > Do employers consider lab techs to be proficient enough? I've been > doing ISH and immuno for 13 years, but I'm not certified as I do research. Maybe there aren't a lot of lab techs out there? Just wondering since you might be missing someone awesome by hiring only certified people. > > "By bitching and bitching and bitching, they could exhaust the drama > of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." > > -Chuck Palahniuk, "Haunted" > > > On Mon, Sep 9, 2013 at 6:49 PM, joelle weaver > >wrote: > > > All I have is a "histology assistant" description I put together. It > > is mostly clerical, instrument up keep and other duties. My employer > > does not hire uncertified techs- due to CLIA license, grossing, FISH > > & molecular duties ( high complexity). I hope to be a clinical site > > eventually, and then they will have to complete their certification > > within one year -if they are hired on. > > If you think that will help, I can send it on. > > > > > > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > > > Date: Mon, 9 Sep 2013 15:39:12 -0500 > > > From: delsuec@gmail.com > > > To: histonet@lists.utsouthwestern.edu< mailto:histonet@lists.utsouthwestern.edu> > > > Subject: [Histonet] Unregistered HT > > > > > > Hi all, > > > > > > I'm wondering if anyone can share a job description for an > > > unregistered > > HT > > > position. I also need to know if unregistered HT's are allowed to > > > cut frozen sections. I know there is a limit to what they can do, > > > especially where IHC and special staining are concerned, but I > > > really don't know > > about > > > frozens, since it's a lot like regular microtomy, except for the > > > H&E staining of the slide. I also understand that facilities might > > > have different descriptions, but what I'm really looking for is > > > what CAP > > allows > > > them to do. > > > > > > Thanks for your help, > > > > > > Deloris Carter, HT(ASCP) > > > SMMC > > > Shawnee, KS > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu< mailto:Histonet@lists.utsouthwestern.edu> > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu< mailto:Histonet@lists.utsouthwestern.edu> > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu< mailto:Histonet@lists.utsouthwestern.edu> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ---------------------------------------------------------------------- > Confidentiality Notice: This e-mail message, including any > attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From Joyce.Weems <@t> emoryhealthcare.org Wed Sep 11 08:22:59 2013 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Wed Sep 11 08:23:09 2013 Subject: [Histonet] (no subject) In-Reply-To: References: Message-ID: Yes, regulations are set up that must be followed. What I did in this situation, was request the slides afterward for review by "my" doctors. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph?s Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of clay milks Sent: Thursday, September 12, 2013 8:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) I need a little help on a Patient?s rights question. It is my understanding that when a patient has a procedure, the patient has the right to request that those specimens be examined by a laboratory of their choosing. i.e. EGD, colonoscopy, etc I am in Arkansas. My father had a procedure yesterday at a local hospital. I manage a pathology laboratory that specializes in the type of tissue that the procedure procured. When it was requested that the tissue be sent to my laboratory, the hospital staff refused to fulfill the request. We asked multiple times for a release form so my father could take his tissue with him. The administration employee we spoke to said there was no such thing and that patients were not allowed to take their specimens. One employee going so far as stating that if we wanted the tissue sent somewhere aside from their contracted laboratory, that the procedure would be canceled and my father would have to go somewhere else. My father, not wanting to cause a fuss, let the issue go. Questions: Is it legal for a hospital to require that tissue specimens be sent to a lab they are contracted with? Are there any other actions we could have taken to make our requests be honored? This is not meant to insult the hospital, but to give an explanation of the situation for context in answering the questions. ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From HornHV <@t> archildrens.org Wed Sep 11 08:29:42 2013 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Wed Sep 11 08:29:50 2013 Subject: [Histonet] Unregistered HT In-Reply-To: References: , , , <41D3A1AF6FEF0643BDC89E0516A6EA32D31581E1@Mail2Node2.ad.uams.edu> <41D3A1AF6FEF0643BDC89E0516A6EA32D3158371@Mail2Node2.ad.uams.edu> Message-ID: <25A4DE08332B19499904459F00AAACB719C704FD68@EVS1.archildrens.org> Histology does not get the respect or the recognition because histologists do not report results. All of the complex testing we do is overlooked because the pathologists report the results. CLIA standards are based on result reporting. The CAP has looked the other way for years because pathologists would hire unregistered techs. If pathologists would demand only registered techs half our battle would be won. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Wednesday, September 11, 2013 8:08 AM To: 'Jennifer MacDonald'; Marcum, Pamela A Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT And the reason so many have been fighting for this for years. If a lab were looking for a Medical Technologist there would be no question. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Tuesday, September 10, 2013 7:32 PM To: Marcum, Pamela A Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT As long as we do not need certification, licensure and minium education requirements we will not be recognized as Laboratory Professionals. From: "Marcum, Pamela A" To: "'joelle weaver'" , "'Emily Sours'" , "histonet@lists.utsouthwestern.edu" Date: 09/10/2013 01:12 PM Subject: RE: [Histonet] Unregistered HT Sent by: histonet-bounces@lists.utsouthwestern.edu I agree we have huge gray areas and not all histology schools are as good as they could be for what we are facing in Histology. I keep harping on the fact that until we are recognized as Laboratory Professionals we will stay in this limbo. The rules determining complex testing should be revisited to what is done in Histology Laboratories today and not what we did 30 or more years ago. The Clinical Laboratory is now so automated it is hard to find anyone in most areas who can even remember doing any manual testing. The Micro lab is the closest to being as manual as areas of Histology. I am in a small market and finding a registered Histologist is harder for us. I would love to have 8 to choose from and interview. Pam Marcum From: joelle weaver [mailto:joelleweaver@hotmail.com] Sent: Tuesday, September 10, 2013 2:59 PM To: Marcum, Pamela A; 'Emily Sours'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT Well I am mostly clinical...but I think that organizations can set standards outside and beyond what CAP,CLIA etc stipulate. For the position I have now, I had to submit all my transcripts from high school up through masters in addition to proof of my ASCP certification, IHC qualification, continuing education, and professional association activity. There is a lot of gray area out there. They seem to have not had trouble getting applicants though ( and I know this varies by market), there were over 8 candidates for an HT opening, which I thought was a pretty good turn out. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: PAMarcum@uams.edu > To: talulahgosh@gmail.com; joelleweaver@hotmail.com > CC: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Unregistered HT > Date: Tue, 10 Sep 2013 13:53:26 +0000 > > Research is a different area and not controlled by CAP, CLIA and other hospital licensing or accreditation organizations. We are bound by the rules of these organizations and while I agree with you to a point. We do need minimums for training and registration by recognized licensing bodies when patient tissue is being processed for histological examination. I am sure no one thinks of this often however; there are medical legal issues with insurance we have that do not apply for research. It is also clear that registration does not mean we don't have registered people who are not as good as they should be for excellent patient care. > > I have worked in research and while I would not ever say the hiring of non-registered people is a problem for research. It is often a specialty area that requires knowing more than routine Histology. I have done plastics in research that could not ever be used in routine Histology due to the time factors and in some cases limited use with staining applications, especially IHC for some procedures. Many other areas in research require more specialized training than would be used in a routine area. I would also add some really great techs are in many phases of research. I know MT who work in Histology and are not registered as the MT BS, overrides the HT requirement for many institutions. > > Many factors must be considered for both research and routine > Histology that cover far more than just hiring registered people in certain areas of the laboratory. > > Pam Marcum > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu< mailto:histonet-bounces@lists.utsouthwestern.edu> [ mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours > Sent: Tuesday, September 10, 2013 8:20 AM > To: joelle weaver > Cc: histonet@lists.utsouthwestern.edu< mailto:histonet@lists.utsouthwestern.edu> > Subject: Re: [Histonet] Unregistered HT > > Do employers consider lab techs to be proficient enough? I've been > doing ISH and immuno for 13 years, but I'm not certified as I do research. Maybe there aren't a lot of lab techs out there? Just wondering since you might be missing someone awesome by hiring only certified people. > > "By bitching and bitching and bitching, they could exhaust the drama > of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." > > -Chuck Palahniuk, "Haunted" > > > On Mon, Sep 9, 2013 at 6:49 PM, joelle weaver > >wrote: > > > All I have is a "histology assistant" description I put together. It > > is mostly clerical, instrument up keep and other duties. My employer > > does not hire uncertified techs- due to CLIA license, grossing, FISH > > & molecular duties ( high complexity). I hope to be a clinical site > > eventually, and then they will have to complete their certification > > within one year -if they are hired on. > > If you think that will help, I can send it on. > > > > > > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > > > Date: Mon, 9 Sep 2013 15:39:12 -0500 > > > From: delsuec@gmail.com > > > To: histonet@lists.utsouthwestern.edu< mailto:histonet@lists.utsouthwestern.edu> > > > Subject: [Histonet] Unregistered HT > > > > > > Hi all, > > > > > > I'm wondering if anyone can share a job description for an > > > unregistered > > HT > > > position. I also need to know if unregistered HT's are allowed to > > > cut frozen sections. I know there is a limit to what they can do, > > > especially where IHC and special staining are concerned, but I > > > really don't know > > about > > > frozens, since it's a lot like regular microtomy, except for the > > > H&E staining of the slide. I also understand that facilities might > > > have different descriptions, but what I'm really looking for is > > > what CAP > > allows > > > them to do. > > > > > > Thanks for your help, > > > > > > Deloris Carter, HT(ASCP) > > > SMMC > > > Shawnee, KS > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu< mailto:Histonet@lists.utsouthwestern.edu> > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu< mailto:Histonet@lists.utsouthwestern.edu> > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu< mailto:Histonet@lists.utsouthwestern.edu> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ---------------------------------------------------------------------- > Confidentiality Notice: This e-mail message, including any > attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From Joyce.Weems <@t> emoryhealthcare.org Wed Sep 11 08:39:03 2013 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Wed Sep 11 08:39:10 2013 Subject: [Histonet] Unregistered HT In-Reply-To: <25A4DE08332B19499904459F00AAACB719C704FD68@EVS1.archildrens.org> References: , , , <41D3A1AF6FEF0643BDC89E0516A6EA32D31581E1@Mail2Node2.ad.uams.edu> <41D3A1AF6FEF0643BDC89E0516A6EA32D3158371@Mail2Node2.ad.uams.edu> <25A4DE08332B19499904459F00AAACB719C704FD68@EVS1.archildrens.org> Message-ID: They are only as good as we are, so we need them to fight for us!! Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: Horn, Hazel V [mailto:HornHV@archildrens.org] Sent: Wednesday, September 11, 2013 9:30 AM To: Weems, Joyce K.; 'Jennifer MacDonald'; Marcum, Pamela A Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT Histology does not get the respect or the recognition because histologists do not report results. All of the complex testing we do is overlooked because the pathologists report the results. CLIA standards are based on result reporting. The CAP has looked the other way for years because pathologists would hire unregistered techs. If pathologists would demand only registered techs half our battle would be won. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Wednesday, September 11, 2013 8:08 AM To: 'Jennifer MacDonald'; Marcum, Pamela A Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT And the reason so many have been fighting for this for years. If a lab were looking for a Medical Technologist there would be no question. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Tuesday, September 10, 2013 7:32 PM To: Marcum, Pamela A Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT As long as we do not need certification, licensure and minium education requirements we will not be recognized as Laboratory Professionals. From: "Marcum, Pamela A" To: "'joelle weaver'" , "'Emily Sours'" , "histonet@lists.utsouthwestern.edu" Date: 09/10/2013 01:12 PM Subject: RE: [Histonet] Unregistered HT Sent by: histonet-bounces@lists.utsouthwestern.edu I agree we have huge gray areas and not all histology schools are as good as they could be for what we are facing in Histology. I keep harping on the fact that until we are recognized as Laboratory Professionals we will stay in this limbo. The rules determining complex testing should be revisited to what is done in Histology Laboratories today and not what we did 30 or more years ago. The Clinical Laboratory is now so automated it is hard to find anyone in most areas who can even remember doing any manual testing. The Micro lab is the closest to being as manual as areas of Histology. I am in a small market and finding a registered Histologist is harder for us. I would love to have 8 to choose from and interview. Pam Marcum From: joelle weaver [mailto:joelleweaver@hotmail.com] Sent: Tuesday, September 10, 2013 2:59 PM To: Marcum, Pamela A; 'Emily Sours'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT Well I am mostly clinical...but I think that organizations can set standards outside and beyond what CAP,CLIA etc stipulate. For the position I have now, I had to submit all my transcripts from high school up through masters in addition to proof of my ASCP certification, IHC qualification, continuing education, and professional association activity. There is a lot of gray area out there. They seem to have not had trouble getting applicants though ( and I know this varies by market), there were over 8 candidates for an HT opening, which I thought was a pretty good turn out. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: PAMarcum@uams.edu > To: talulahgosh@gmail.com; joelleweaver@hotmail.com > CC: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Unregistered HT > Date: Tue, 10 Sep 2013 13:53:26 +0000 > > Research is a different area and not controlled by CAP, CLIA and other hospital licensing or accreditation organizations. We are bound by the rules of these organizations and while I agree with you to a point. We do need minimums for training and registration by recognized licensing bodies when patient tissue is being processed for histological examination. I am sure no one thinks of this often however; there are medical legal issues with insurance we have that do not apply for research. It is also clear that registration does not mean we don't have registered people who are not as good as they should be for excellent patient care. > > I have worked in research and while I would not ever say the hiring of non-registered people is a problem for research. It is often a specialty area that requires knowing more than routine Histology. I have done plastics in research that could not ever be used in routine Histology due to the time factors and in some cases limited use with staining applications, especially IHC for some procedures. Many other areas in research require more specialized training than would be used in a routine area. I would also add some really great techs are in many phases of research. I know MT who work in Histology and are not registered as the MT BS, overrides the HT requirement for many institutions. > > Many factors must be considered for both research and routine > Histology that cover far more than just hiring registered people in certain areas of the laboratory. > > Pam Marcum > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu< mailto:histonet-bounces@lists.utsouthwestern.edu> [ mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours > Sent: Tuesday, September 10, 2013 8:20 AM > To: joelle weaver > Cc: histonet@lists.utsouthwestern.edu< mailto:histonet@lists.utsouthwestern.edu> > Subject: Re: [Histonet] Unregistered HT > > Do employers consider lab techs to be proficient enough? I've been > doing ISH and immuno for 13 years, but I'm not certified as I do research. Maybe there aren't a lot of lab techs out there? Just wondering since you might be missing someone awesome by hiring only certified people. > > "By bitching and bitching and bitching, they could exhaust the drama > of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." > > -Chuck Palahniuk, "Haunted" > > > On Mon, Sep 9, 2013 at 6:49 PM, joelle weaver > >wrote: > > > All I have is a "histology assistant" description I put together. It > > is mostly clerical, instrument up keep and other duties. My employer > > does not hire uncertified techs- due to CLIA license, grossing, FISH > > & molecular duties ( high complexity). I hope to be a clinical site > > eventually, and then they will have to complete their certification > > within one year -if they are hired on. > > If you think that will help, I can send it on. > > > > > > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > > > Date: Mon, 9 Sep 2013 15:39:12 -0500 > > > From: delsuec@gmail.com > > > To: histonet@lists.utsouthwestern.edu< mailto:histonet@lists.utsouthwestern.edu> > > > Subject: [Histonet] Unregistered HT > > > > > > Hi all, > > > > > > I'm wondering if anyone can share a job description for an > > > unregistered > > HT > > > position. I also need to know if unregistered HT's are allowed to > > > cut frozen sections. I know there is a limit to what they can do, > > > especially where IHC and special staining are concerned, but I > > > really don't know > > about > > > frozens, since it's a lot like regular microtomy, except for the > > > H&E staining of the slide. I also understand that facilities might > > > have different descriptions, but what I'm really looking for is > > > what CAP > > allows > > > them to do. > > > > > > Thanks for your help, > > > > > > Deloris Carter, HT(ASCP) > > > SMMC > > > Shawnee, KS > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu< mailto:Histonet@lists.utsouthwestern.edu> > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu< mailto:Histonet@lists.utsouthwestern.edu> > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu< mailto:Histonet@lists.utsouthwestern.edu> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ---------------------------------------------------------------------- > Confidentiality Notice: This e-mail message, including any > attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From PAMarcum <@t> uams.edu Wed Sep 11 08:40:32 2013 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Wed Sep 11 08:40:39 2013 Subject: [Histonet] Unregistered HT In-Reply-To: <25A4DE08332B19499904459F00AAACB719C704FD68@EVS1.archildrens.org> References: , , , <41D3A1AF6FEF0643BDC89E0516A6EA32D31581E1@Mail2Node2.ad.uams.edu> <41D3A1AF6FEF0643BDC89E0516A6EA32D3158371@Mail2Node2.ad.uams.edu> <25A4DE08332B19499904459F00AAACB719C704FD68@EVS1.archildrens.org> Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA32D315851A@Mail2Node2.ad.uams.edu> You are absolutely right. Pathologists are afraid if they are not seen as the ones doing "complex testing" the last bastion of their power will disappear. It would help if they just realize how much we do to help them by knowing how to read and judge whether those "complex tests" are done right and the correct controls in place to keep them from saying or reading the wrong thing. Many pathologists will admit they could not do our job or even understand what we do to get the tissue from a cassette to a stained slide. I think some think we wiggle our noses and it appears like magic stained on the slide. If they can't gross well, then we know where the true understanding of Histology starts. They managed to get a registry for Pathology Assistants that is quite hard to pass so they could stop grossing in many hospitals. Now we have some good and some bad PAs who are of the same opinion as the pathologists about Histologists in many cases. We have CLIA approved (based on education and number of science hours) as the cost of PA is too high in many AP labs. Pam Marcum. -----Original Message----- From: Horn, Hazel V [mailto:HornHV@archildrens.org] Sent: Wednesday, September 11, 2013 8:30 AM To: 'Weems, Joyce K.'; 'Jennifer MacDonald'; Marcum, Pamela A Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT Histology does not get the respect or the recognition because histologists do not report results. All of the complex testing we do is overlooked because the pathologists report the results. CLIA standards are based on result reporting. The CAP has looked the other way for years because pathologists would hire unregistered techs. If pathologists would demand only registered techs half our battle would be won. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Wednesday, September 11, 2013 8:08 AM To: 'Jennifer MacDonald'; Marcum, Pamela A Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT And the reason so many have been fighting for this for years. If a lab were looking for a Medical Technologist there would be no question. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Tuesday, September 10, 2013 7:32 PM To: Marcum, Pamela A Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT As long as we do not need certification, licensure and minium education requirements we will not be recognized as Laboratory Professionals. From: "Marcum, Pamela A" To: "'joelle weaver'" , "'Emily Sours'" , "histonet@lists.utsouthwestern.edu" Date: 09/10/2013 01:12 PM Subject: RE: [Histonet] Unregistered HT Sent by: histonet-bounces@lists.utsouthwestern.edu I agree we have huge gray areas and not all histology schools are as good as they could be for what we are facing in Histology. I keep harping on the fact that until we are recognized as Laboratory Professionals we will stay in this limbo. The rules determining complex testing should be revisited to what is done in Histology Laboratories today and not what we did 30 or more years ago. The Clinical Laboratory is now so automated it is hard to find anyone in most areas who can even remember doing any manual testing. The Micro lab is the closest to being as manual as areas of Histology. I am in a small market and finding a registered Histologist is harder for us. I would love to have 8 to choose from and interview. Pam Marcum From: joelle weaver [mailto:joelleweaver@hotmail.com] Sent: Tuesday, September 10, 2013 2:59 PM To: Marcum, Pamela A; 'Emily Sours'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT Well I am mostly clinical...but I think that organizations can set standards outside and beyond what CAP,CLIA etc stipulate. For the position I have now, I had to submit all my transcripts from high school up through masters in addition to proof of my ASCP certification, IHC qualification, continuing education, and professional association activity. There is a lot of gray area out there. They seem to have not had trouble getting applicants though ( and I know this varies by market), there were over 8 candidates for an HT opening, which I thought was a pretty good turn out. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: PAMarcum@uams.edu > To: talulahgosh@gmail.com; joelleweaver@hotmail.com > CC: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Unregistered HT > Date: Tue, 10 Sep 2013 13:53:26 +0000 > > Research is a different area and not controlled by CAP, CLIA and other hospital licensing or accreditation organizations. We are bound by the rules of these organizations and while I agree with you to a point. We do need minimums for training and registration by recognized licensing bodies when patient tissue is being processed for histological examination. I am sure no one thinks of this often however; there are medical legal issues with insurance we have that do not apply for research. It is also clear that registration does not mean we don't have registered people who are not as good as they should be for excellent patient care. > > I have worked in research and while I would not ever say the hiring of non-registered people is a problem for research. It is often a specialty area that requires knowing more than routine Histology. I have done plastics in research that could not ever be used in routine Histology due to the time factors and in some cases limited use with staining applications, especially IHC for some procedures. Many other areas in research require more specialized training than would be used in a routine area. I would also add some really great techs are in many phases of research. I know MT who work in Histology and are not registered as the MT BS, overrides the HT requirement for many institutions. > > Many factors must be considered for both research and routine > Histology that cover far more than just hiring registered people in certain areas of the laboratory. > > Pam Marcum > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu< mailto:histonet-bounces@lists.utsouthwestern.edu> [ mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours > Sent: Tuesday, September 10, 2013 8:20 AM > To: joelle weaver > Cc: histonet@lists.utsouthwestern.edu< mailto:histonet@lists.utsouthwestern.edu> > Subject: Re: [Histonet] Unregistered HT > > Do employers consider lab techs to be proficient enough? I've been > doing ISH and immuno for 13 years, but I'm not certified as I do research. Maybe there aren't a lot of lab techs out there? Just wondering since you might be missing someone awesome by hiring only certified people. > > "By bitching and bitching and bitching, they could exhaust the drama > of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." > > -Chuck Palahniuk, "Haunted" > > > On Mon, Sep 9, 2013 at 6:49 PM, joelle weaver > >wrote: > > > All I have is a "histology assistant" description I put together. It > > is mostly clerical, instrument up keep and other duties. My employer > > does not hire uncertified techs- due to CLIA license, grossing, FISH > > & molecular duties ( high complexity). I hope to be a clinical site > > eventually, and then they will have to complete their certification > > within one year -if they are hired on. > > If you think that will help, I can send it on. > > > > > > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > > > Date: Mon, 9 Sep 2013 15:39:12 -0500 > > > From: delsuec@gmail.com > > > To: histonet@lists.utsouthwestern.edu< mailto:histonet@lists.utsouthwestern.edu> > > > Subject: [Histonet] Unregistered HT > > > > > > Hi all, > > > > > > I'm wondering if anyone can share a job description for an > > > unregistered > > HT > > > position. I also need to know if unregistered HT's are allowed to > > > cut frozen sections. I know there is a limit to what they can do, > > > especially where IHC and special staining are concerned, but I > > > really don't know > > about > > > frozens, since it's a lot like regular microtomy, except for the > > > H&E staining of the slide. I also understand that facilities might > > > have different descriptions, but what I'm really looking for is > > > what CAP > > allows > > > them to do. > > > > > > Thanks for your help, > > > > > > Deloris Carter, HT(ASCP) > > > SMMC > > > Shawnee, KS > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu< mailto:Histonet@lists.utsouthwestern.edu> > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu< mailto:Histonet@lists.utsouthwestern.edu> > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu< mailto:Histonet@lists.utsouthwestern.edu> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ---------------------------------------------------------------------- > Confidentiality Notice: This e-mail message, including any > attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From mucram11 <@t> comcast.net Wed Sep 11 08:45:35 2013 From: mucram11 <@t> comcast.net (Pam Marcum) Date: Wed Sep 11 08:45:55 2013 Subject: [Histonet] Unregistered HT In-Reply-To: Message-ID: <484323625.958457.1378907135764.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> We also need NSH to step up and do what they said they would years ago instead of planning more and more meetings per year and increasing what we pay them for not doing anything to help us.? They seem to support anything ASCP/CAP/CLIA etc tells them and we are left footing the bill in more ways than monetarily.? NSH in Providence is in a week and I cannot go this time so why can't those going start asking where the proposal brought up years ago hasn't moved forward.? I would have been happy to bring it up from the floor in the general membership meeting.? Pam Marcum ----- Original Message ----- From: "Joyce K. Weems" To: histonet@lists.utsouthwestern.edu Sent: Wednesday, September 11, 2013 8:39:03 AM Subject: RE: [Histonet] Unregistered HT They are only as good as we are, so we need them to fight for us!! Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). ?It may contain information that is privileged and confidential. ?Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: Horn, Hazel V [mailto:HornHV@archildrens.org] Sent: Wednesday, September 11, 2013 9:30 AM To: Weems, Joyce K.; 'Jennifer MacDonald'; Marcum, Pamela A Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT Histology does not get the respect or the recognition because histologists do not report results. ?All of the complex testing we do is overlooked because the pathologists report the results. ? CLIA standards are based on result reporting. ? The CAP has looked the other way for years because pathologists would hire unregistered techs. ?If pathologists would demand only registered techs half our battle would be won. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Wednesday, September 11, 2013 8:08 AM To: 'Jennifer MacDonald'; Marcum, Pamela A Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT And the reason so many have been fighting for this for years. If a lab were looking for a Medical Technologist there would be no question. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). ?It may contain information that is privileged and confidential. ?Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Tuesday, September 10, 2013 7:32 PM To: Marcum, Pamela A Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT As long as we do not need certification, licensure and minium education requirements we will not be recognized as Laboratory Professionals. From: ? "Marcum, Pamela A" To: ? ? "'joelle weaver'" , "'Emily Sours'" , "histonet@lists.utsouthwestern.edu" Date: ? 09/10/2013 01:12 PM Subject: ? ? ? ?RE: [Histonet] Unregistered HT Sent by: ? ? ? ?histonet-bounces@lists.utsouthwestern.edu I agree we have huge gray areas and not all histology schools are as good as they could be for what we are facing in Histology. ?I keep harping on the fact that until we are recognized as Laboratory Professionals we will stay in this limbo. ?The rules determining complex testing should be revisited to what is done in Histology Laboratories today and not what we did 30 or more years ago. ?The Clinical Laboratory is now so automated it is hard to find anyone in most areas who can even remember doing any manual testing. ?The Micro lab is the closest to being as manual as areas of Histology. I am in a small market and finding a registered Histologist is harder for us. ?I would love to have 8 to choose from and interview. Pam Marcum From: joelle weaver [mailto:joelleweaver@hotmail.com] Sent: Tuesday, September 10, 2013 2:59 PM To: Marcum, Pamela A; 'Emily Sours'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT ?Well I am ?mostly clinical...but I think that organizations can set standards outside and beyond what CAP,CLIA etc stipulate. For the position I have now, I had to submit all my transcripts from high school up through masters in addition to ?proof of my ASCP certification, IHC qualification, continuing education, and professional association activity. There is a lot of gray area out there. They seem to have not had trouble getting applicants though ( and I know this varies by market), there were over 8 candidates for an HT opening, which I thought was a pretty good turn out. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: PAMarcum@uams.edu > To: talulahgosh@gmail.com; joelleweaver@hotmail.com > CC: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Unregistered HT > Date: Tue, 10 Sep 2013 13:53:26 +0000 > > Research is a different area and not controlled by CAP, CLIA and other hospital licensing or accreditation organizations. We are bound by the rules of these organizations and while I agree with you to a point. We do need minimums for training and registration by recognized licensing bodies when patient tissue is being processed for histological examination. I am sure no one thinks of this often however; there are medical legal issues with insurance we have that do not apply for research. It is also clear that registration does not mean we don't have registered people who are not as good as they should be for excellent patient care. > > I have worked in research and while I would not ever say the hiring of non-registered people is a problem for research. It is often a specialty area that requires knowing more than routine Histology. I have done plastics in research that could not ever be used in routine Histology due to the time factors and in some cases limited use with staining applications, especially IHC for some procedures. Many other areas in research require more specialized training than would be used in a routine area. I would also add some really great techs are in many phases of research. I know MT who work in Histology and are not registered as the MT BS, overrides the HT requirement for many institutions. > > Many factors must be considered for both research and routine > Histology that cover far more than just hiring registered people in certain areas of the laboratory. > > Pam Marcum > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu< mailto:histonet-bounces@lists.utsouthwestern.edu> [ mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours > Sent: Tuesday, September 10, 2013 8:20 AM > To: joelle weaver > Cc: histonet@lists.utsouthwestern.edu< mailto:histonet@lists.utsouthwestern.edu> > Subject: Re: [Histonet] Unregistered HT > > Do employers consider lab techs to be proficient enough? I've been > doing ISH and immuno for 13 years, but I'm not certified as I do research. Maybe there aren't a lot of lab techs out there? Just wondering since you might be missing someone awesome by hiring only certified people. > > "By bitching and bitching and bitching, they could exhaust the drama > of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." > > -Chuck Palahniuk, "Haunted" > > > On Mon, Sep 9, 2013 at 6:49 PM, joelle weaver > >wrote: > > > All I have is a "histology assistant" description I put together. It > > is mostly clerical, instrument up keep and other duties. My employer > > does not hire uncertified techs- due to CLIA license, grossing, FISH > > & molecular duties ( high complexity). I hope to be a clinical site > > eventually, and then they will have to complete their certification > > within one year -if they are hired on. > > If you think that will help, I can send it on. > > > > > > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > > > Date: Mon, 9 Sep 2013 15:39:12 -0500 > > > From: delsuec@gmail.com > > > To: histonet@lists.utsouthwestern.edu< mailto:histonet@lists.utsouthwestern.edu> > > > Subject: [Histonet] Unregistered HT > > > > > > Hi all, > > > > > > I'm wondering if anyone can share a job description for an > > > unregistered > > HT > > > position. I also need to know if unregistered HT's are allowed to > > > cut frozen sections. I know there is a limit to what they can do, > > > especially where IHC and special staining are concerned, but I > > > really don't know > > about > > > frozens, since it's a lot like regular microtomy, except for the > > > H&E staining of the slide. I also understand that facilities might > > > have different descriptions, but what I'm really looking for is > > > what CAP > > allows > > > them to do. > > > > > > Thanks for your help, > > > > > > Deloris Carter, HT(ASCP) > > > SMMC > > > Shawnee, KS > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu< mailto:Histonet@lists.utsouthwestern.edu> > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu< mailto:Histonet@lists.utsouthwestern.edu> > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu< mailto:Histonet@lists.utsouthwestern.edu> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ---------------------------------------------------------------------- > Confidentiality Notice: This e-mail message, including any > attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From talulahgosh <@t> gmail.com Wed Sep 11 08:58:06 2013 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Wed Sep 11 08:58:10 2013 Subject: [Histonet] Unregistered HT In-Reply-To: References: <41D3A1AF6FEF0643BDC89E0516A6EA32D31581E1@Mail2Node2.ad.uams.edu> <41D3A1AF6FEF0643BDC89E0516A6EA32D3158371@Mail2Node2.ad.uams.edu> Message-ID: Do you get paid more with that title? Just curious, I'm not sure what "laboratory professional" means, as I'm not in that kind of lab. Or is it a respect thing? Emily "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." -Chuck Palahniuk, "Haunted" On Tue, Sep 10, 2013 at 7:32 PM, Jennifer MacDonald wrote: > As long as we do not need certification, licensure and minium education > requirements we will not be recognized as Laboratory Professionals. > > From CThornton <@t> dahlchase.com Wed Sep 11 09:20:20 2013 From: CThornton <@t> dahlchase.com (Clare Thornton) Date: Wed Sep 11 09:20:28 2013 Subject: [Histonet] Unregistered HT In-Reply-To: References: <41D3A1AF6FEF0643BDC89E0516A6EA32D31581E1@Mail2Node2.ad.uams.edu> <41D3A1AF6FEF0643BDC89E0516A6EA32D3158371@Mail2Node2.ad.uams.edu> Message-ID: Look for a position paper presented by members of the NSH BOD in the Journal of Histotechnology after the national convention in Vancouver last year. I'm not sure of the exact issue (thought I still had it on my desk). This will explain what being a laboratory professional is and why it is so important that as histotechnologists we are viewed as such. Clare J. Thornton, HTL(ASCP)QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Wednesday, September 11, 2013 9:58 AM Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Unregistered HT Do you get paid more with that title? Just curious, I'm not sure what "laboratory professional" means, as I'm not in that kind of lab. Or is it a respect thing? Emily "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." -Chuck Palahniuk, "Haunted" On Tue, Sep 10, 2013 at 7:32 PM, Jennifer MacDonald wrote: > As long as we do not need certification, licensure and minium > education requirements we will not be recognized as Laboratory Professionals. > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMitchell <@t> uwhealth.org Wed Sep 11 09:26:02 2013 From: JMitchell <@t> uwhealth.org (Mitchell Jean A) Date: Wed Sep 11 09:26:09 2013 Subject: [Histonet] Unregistered HT In-Reply-To: <25A4DE08332B19499904459F00AAACB719C704FD68@EVS1.archildrens.org> References: , , , <41D3A1AF6FEF0643BDC89E0516A6EA32D31581E1@Mail2Node2.ad.uams.edu> <41D3A1AF6FEF0643BDC89E0516A6EA32D3158371@Mail2Node2.ad.uams.edu> <25A4DE08332B19499904459F00AAACB719C704FD68@EVS1.archildrens.org> Message-ID: <16F90B93CA23D446980B3D591FD02DAD08A835@UWHC-MBX14.uwhis.hosp.wisc.edu> Even if a facility does not require that techs be HT registered - the technicians themselves need go that extra step, take the initiative themselves and become HT certified. If a portion of histologists don't recognize or care about the merit of certification how can we progress our profession to the status it deserves? You have to believe in yourself in order for others to follow suit. With the exclusion of the front office; NSH is a volunteer organization that fights for our profession and it can be difficult to take on the big dogs of ASCP/CAP/CLIA. I like to see this passion for our profession and if we keep this up eventually we will see results and the professional status we deserve. Jean Mitchell, BS HT (ASCP) University of Wisconsin Hospital & Clinics Neuromuscular Laboratory 600 Highland Avenue Madison, WI 53792-5132 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Wednesday, September 11, 2013 8:30 AM To: 'Weems, Joyce K.'; 'Jennifer MacDonald'; Marcum, Pamela A Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT Histology does not get the respect or the recognition because histologists do not report results. All of the complex testing we do is overlooked because the pathologists report the results. CLIA standards are based on result reporting. The CAP has looked the other way for years because pathologists would hire unregistered techs. If pathologists would demand only registered techs half our battle would be won. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Wednesday, September 11, 2013 8:08 AM To: 'Jennifer MacDonald'; Marcum, Pamela A Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT And the reason so many have been fighting for this for years. If a lab were looking for a Medical Technologist there would be no question. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Tuesday, September 10, 2013 7:32 PM To: Marcum, Pamela A Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT As long as we do not need certification, licensure and minium education requirements we will not be recognized as Laboratory Professionals. From: "Marcum, Pamela A" To: "'joelle weaver'" , "'Emily Sours'" , "histonet@lists.utsouthwestern.edu" Date: 09/10/2013 01:12 PM Subject: RE: [Histonet] Unregistered HT Sent by: histonet-bounces@lists.utsouthwestern.edu I agree we have huge gray areas and not all histology schools are as good as they could be for what we are facing in Histology. I keep harping on the fact that until we are recognized as Laboratory Professionals we will stay in this limbo. The rules determining complex testing should be revisited to what is done in Histology Laboratories today and not what we did 30 or more years ago. The Clinical Laboratory is now so automated it is hard to find anyone in most areas who can even remember doing any manual testing. The Micro lab is the closest to being as manual as areas of Histology. I am in a small market and finding a registered Histologist is harder for us. I would love to have 8 to choose from and interview. Pam Marcum From: joelle weaver [mailto:joelleweaver@hotmail.com] Sent: Tuesday, September 10, 2013 2:59 PM To: Marcum, Pamela A; 'Emily Sours'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT Well I am mostly clinical...but I think that organizations can set standards outside and beyond what CAP,CLIA etc stipulate. For the position I have now, I had to submit all my transcripts from high school up through masters in addition to proof of my ASCP certification, IHC qualification, continuing education, and professional association activity. There is a lot of gray area out there. They seem to have not had trouble getting applicants though ( and I know this varies by market), there were over 8 candidates for an HT opening, which I thought was a pretty good turn out. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: PAMarcum@uams.edu > To: talulahgosh@gmail.com; joelleweaver@hotmail.com > CC: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Unregistered HT > Date: Tue, 10 Sep 2013 13:53:26 +0000 > > Research is a different area and not controlled by CAP, CLIA and other hospital licensing or accreditation organizations. We are bound by the rules of these organizations and while I agree with you to a point. We do need minimums for training and registration by recognized licensing bodies when patient tissue is being processed for histological examination. I am sure no one thinks of this often however; there are medical legal issues with insurance we have that do not apply for research. It is also clear that registration does not mean we don't have registered people who are not as good as they should be for excellent patient care. > > I have worked in research and while I would not ever say the hiring of non-registered people is a problem for research. It is often a specialty area that requires knowing more than routine Histology. I have done plastics in research that could not ever be used in routine Histology due to the time factors and in some cases limited use with staining applications, especially IHC for some procedures. Many other areas in research require more specialized training than would be used in a routine area. I would also add some really great techs are in many phases of research. I know MT who work in Histology and are not registered as the MT BS, overrides the HT requirement for many institutions. > > Many factors must be considered for both research and routine > Histology that cover far more than just hiring registered people in certain areas of the laboratory. > > Pam Marcum > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu< mailto:histonet-bounces@lists.utsouthwestern.edu> [ mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours > Sent: Tuesday, September 10, 2013 8:20 AM > To: joelle weaver > Cc: histonet@lists.utsouthwestern.edu< mailto:histonet@lists.utsouthwestern.edu> > Subject: Re: [Histonet] Unregistered HT > > Do employers consider lab techs to be proficient enough? I've been > doing ISH and immuno for 13 years, but I'm not certified as I do research. Maybe there aren't a lot of lab techs out there? Just wondering since you might be missing someone awesome by hiring only certified people. > > "By bitching and bitching and bitching, they could exhaust the drama > of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." > > -Chuck Palahniuk, "Haunted" > > > On Mon, Sep 9, 2013 at 6:49 PM, joelle weaver > >wrote: > > > All I have is a "histology assistant" description I put together. It > > is mostly clerical, instrument up keep and other duties. My employer > > does not hire uncertified techs- due to CLIA license, grossing, FISH > > & molecular duties ( high complexity). I hope to be a clinical site > > eventually, and then they will have to complete their certification > > within one year -if they are hired on. > > If you think that will help, I can send it on. > > > > > > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > > > Date: Mon, 9 Sep 2013 15:39:12 -0500 > > > From: delsuec@gmail.com > > > To: histonet@lists.utsouthwestern.edu< mailto:histonet@lists.utsouthwestern.edu> > > > Subject: [Histonet] Unregistered HT > > > > > > Hi all, > > > > > > I'm wondering if anyone can share a job description for an > > > unregistered > > HT > > > position. I also need to know if unregistered HT's are allowed to > > > cut frozen sections. I know there is a limit to what they can do, > > > especially where IHC and special staining are concerned, but I > > > really don't know > > about > > > frozens, since it's a lot like regular microtomy, except for the > > > H&E staining of the slide. I also understand that facilities might > > > have different descriptions, but what I'm really looking for is > > > what CAP > > allows > > > them to do. > > > > > > Thanks for your help, > > > > > > Deloris Carter, HT(ASCP) > > > SMMC > > > Shawnee, KS > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu< mailto:Histonet@lists.utsouthwestern.edu> > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu< mailto:Histonet@lists.utsouthwestern.edu> > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu< mailto:Histonet@lists.utsouthwestern.edu> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ---------------------------------------------------------------------- > Confidentiality Notice: This e-mail message, including any > attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LSebree <@t> uwhealth.org Wed Sep 11 09:31:15 2013 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Wed Sep 11 09:31:23 2013 Subject: [Histonet] Unregistered HT In-Reply-To: <16F90B93CA23D446980B3D591FD02DAD08A835@UWHC-MBX14.uwhis.hosp.wisc.edu> References: , , , <41D3A1AF6FEF0643BDC89E0516A6EA32D31581E1@Mail2Node2.ad.uams.edu> <41D3A1AF6FEF0643BDC89E0516A6EA32D3158371@Mail2Node2.ad.uams.edu> <25A4DE08332B19499904459F00AAACB719C704FD68@EVS1.archildrens.org> <16F90B93CA23D446980B3D591FD02DAD08A835@UWHC-MBX14.uwhis.hosp.wisc.edu> Message-ID: <77DD817201982748BC67D7960F2F76AF065D43@UWHC-MBX12.uwhis.hosp.wisc.edu> Well said Jean. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mitchell Jean A Sent: Wednesday, September 11, 2013 9:26 AM To: 'Horn, Hazel V'; 'Weems, Joyce K.'; 'Jennifer MacDonald'; Marcum, Pamela A Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT Even if a facility does not require that techs be HT registered - the technicians themselves need go that extra step, take the initiative themselves and become HT certified. If a portion of histologists don't recognize or care about the merit of certification how can we progress our profession to the status it deserves? You have to believe in yourself in order for others to follow suit. With the exclusion of the front office; NSH is a volunteer organization that fights for our profession and it can be difficult to take on the big dogs of ASCP/CAP/CLIA. I like to see this passion for our profession and if we keep this up eventually we will see results and the professional status we deserve. Jean Mitchell, BS HT (ASCP) University of Wisconsin Hospital & Clinics Neuromuscular Laboratory 600 Highland Avenue Madison, WI 53792-5132 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Wednesday, September 11, 2013 8:30 AM To: 'Weems, Joyce K.'; 'Jennifer MacDonald'; Marcum, Pamela A Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT Histology does not get the respect or the recognition because histologists do not report results. All of the complex testing we do is overlooked because the pathologists report the results. CLIA standards are based on result reporting. The CAP has looked the other way for years because pathologists would hire unregistered techs. If pathologists would demand only registered techs half our battle would be won. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Wednesday, September 11, 2013 8:08 AM To: 'Jennifer MacDonald'; Marcum, Pamela A Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT And the reason so many have been fighting for this for years. If a lab were looking for a Medical Technologist there would be no question. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Tuesday, September 10, 2013 7:32 PM To: Marcum, Pamela A Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT As long as we do not need certification, licensure and minium education requirements we will not be recognized as Laboratory Professionals. From: "Marcum, Pamela A" To: "'joelle weaver'" , "'Emily Sours'" , "histonet@lists.utsouthwestern.edu" Date: 09/10/2013 01:12 PM Subject: RE: [Histonet] Unregistered HT Sent by: histonet-bounces@lists.utsouthwestern.edu I agree we have huge gray areas and not all histology schools are as good as they could be for what we are facing in Histology. I keep harping on the fact that until we are recognized as Laboratory Professionals we will stay in this limbo. The rules determining complex testing should be revisited to what is done in Histology Laboratories today and not what we did 30 or more years ago. The Clinical Laboratory is now so automated it is hard to find anyone in most areas who can even remember doing any manual testing. The Micro lab is the closest to being as manual as areas of Histology. I am in a small market and finding a registered Histologist is harder for us. I would love to have 8 to choose from and interview. Pam Marcum From: joelle weaver [mailto:joelleweaver@hotmail.com] Sent: Tuesday, September 10, 2013 2:59 PM To: Marcum, Pamela A; 'Emily Sours'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT Well I am mostly clinical...but I think that organizations can set standards outside and beyond what CAP,CLIA etc stipulate. For the position I have now, I had to submit all my transcripts from high school up through masters in addition to proof of my ASCP certification, IHC qualification, continuing education, and professional association activity. There is a lot of gray area out there. They seem to have not had trouble getting applicants though ( and I know this varies by market), there were over 8 candidates for an HT opening, which I thought was a pretty good turn out. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: PAMarcum@uams.edu > To: talulahgosh@gmail.com; joelleweaver@hotmail.com > CC: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Unregistered HT > Date: Tue, 10 Sep 2013 13:53:26 +0000 > > Research is a different area and not controlled by CAP, CLIA and other hospital licensing or accreditation organizations. We are bound by the rules of these organizations and while I agree with you to a point. We do need minimums for training and registration by recognized licensing bodies when patient tissue is being processed for histological examination. I am sure no one thinks of this often however; there are medical legal issues with insurance we have that do not apply for research. It is also clear that registration does not mean we don't have registered people who are not as good as they should be for excellent patient care. > > I have worked in research and while I would not ever say the hiring of non-registered people is a problem for research. It is often a specialty area that requires knowing more than routine Histology. I have done plastics in research that could not ever be used in routine Histology due to the time factors and in some cases limited use with staining applications, especially IHC for some procedures. Many other areas in research require more specialized training than would be used in a routine area. I would also add some really great techs are in many phases of research. I know MT who work in Histology and are not registered as the MT BS, overrides the HT requirement for many institutions. > > Many factors must be considered for both research and routine > Histology that cover far more than just hiring registered people in certain areas of the laboratory. > > Pam Marcum > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu< mailto:histonet-bounces@lists.utsouthwestern.edu> [ mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours > Sent: Tuesday, September 10, 2013 8:20 AM > To: joelle weaver > Cc: histonet@lists.utsouthwestern.edu< mailto:histonet@lists.utsouthwestern.edu> > Subject: Re: [Histonet] Unregistered HT > > Do employers consider lab techs to be proficient enough? I've been > doing ISH and immuno for 13 years, but I'm not certified as I do research. Maybe there aren't a lot of lab techs out there? Just wondering since you might be missing someone awesome by hiring only certified people. > > "By bitching and bitching and bitching, they could exhaust the drama > of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." > > -Chuck Palahniuk, "Haunted" > > > On Mon, Sep 9, 2013 at 6:49 PM, joelle weaver > >wrote: > > > All I have is a "histology assistant" description I put together. It > > is mostly clerical, instrument up keep and other duties. My employer > > does not hire uncertified techs- due to CLIA license, grossing, FISH > > & molecular duties ( high complexity). I hope to be a clinical site > > eventually, and then they will have to complete their certification > > within one year -if they are hired on. > > If you think that will help, I can send it on. > > > > > > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > > > Date: Mon, 9 Sep 2013 15:39:12 -0500 > > > From: delsuec@gmail.com > > > To: histonet@lists.utsouthwestern.edu< mailto:histonet@lists.utsouthwestern.edu> > > > Subject: [Histonet] Unregistered HT > > > > > > Hi all, > > > > > > I'm wondering if anyone can share a job description for an > > > unregistered > > HT > > > position. I also need to know if unregistered HT's are allowed to > > > cut frozen sections. I know there is a limit to what they can do, > > > especially where IHC and special staining are concerned, but I > > > really don't know > > about > > > frozens, since it's a lot like regular microtomy, except for the > > > H&E staining of the slide. I also understand that facilities might > > > have different descriptions, but what I'm really looking for is > > > what CAP > > allows > > > them to do. > > > > > > Thanks for your help, > > > > > > Deloris Carter, HT(ASCP) > > > SMMC > > > Shawnee, KS > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu< mailto:Histonet@lists.utsouthwestern.edu> > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu< mailto:Histonet@lists.utsouthwestern.edu> > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu< mailto:Histonet@lists.utsouthwestern.edu> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ---------------------------------------------------------------------- > Confidentiality Notice: This e-mail message, including any > attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PAMarcum <@t> uams.edu Wed Sep 11 09:46:16 2013 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Wed Sep 11 09:46:26 2013 Subject: [Histonet] Unregistered HT In-Reply-To: <16F90B93CA23D446980B3D591FD02DAD08A835@UWHC-MBX14.uwhis.hosp.wisc.edu> References: , , , <41D3A1AF6FEF0643BDC89E0516A6EA32D31581E1@Mail2Node2.ad.uams.edu> <41D3A1AF6FEF0643BDC89E0516A6EA32D3158371@Mail2Node2.ad.uams.edu> <25A4DE08332B19499904459F00AAACB719C704FD68@EVS1.archildrens.org> <16F90B93CA23D446980B3D591FD02DAD08A835@UWHC-MBX14.uwhis.hosp.wisc.edu> Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA32D3158578@Mail2Node2.ad.uams.edu> Agreed and now that the registries for HT and HTL require credits to remain registered it does help with keeping people in the area learning and growing. The point that other than the front office for NSH is a well taken point and the rest are volunteers. I am not always sure the front office understands hospitals, private labs, universities etc. are not sending anyone to meetings or really encouraging further education for most lab staffs. Histology here can get registration paid for and everything else is on the person attending for transportation/room and board and all other expenses we are not able to go to meetings in far flung places. Pam Marcum -----Original Message----- From: Mitchell Jean A [mailto:JMitchell@uwhealth.org] Sent: Wednesday, September 11, 2013 9:26 AM To: Horn, Hazel V; 'Weems, Joyce K.'; 'Jennifer MacDonald'; Marcum, Pamela A Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT Even if a facility does not require that techs be HT registered - the technicians themselves need go that extra step, take the initiative themselves and become HT certified. If a portion of histologists don't recognize or care about the merit of certification how can we progress our profession to the status it deserves? You have to believe in yourself in order for others to follow suit. With the exclusion of the front office; NSH is a volunteer organization that fights for our profession and it can be difficult to take on the big dogs of ASCP/CAP/CLIA. I like to see this passion for our profession and if we keep this up eventually we will see results and the professional status we deserve. Jean Mitchell, BS HT (ASCP) University of Wisconsin Hospital & Clinics Neuromuscular Laboratory 600 Highland Avenue Madison, WI 53792-5132 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Wednesday, September 11, 2013 8:30 AM To: 'Weems, Joyce K.'; 'Jennifer MacDonald'; Marcum, Pamela A Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT Histology does not get the respect or the recognition because histologists do not report results. All of the complex testing we do is overlooked because the pathologists report the results. CLIA standards are based on result reporting. The CAP has looked the other way for years because pathologists would hire unregistered techs. If pathologists would demand only registered techs half our battle would be won. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Wednesday, September 11, 2013 8:08 AM To: 'Jennifer MacDonald'; Marcum, Pamela A Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT And the reason so many have been fighting for this for years. If a lab were looking for a Medical Technologist there would be no question. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Tuesday, September 10, 2013 7:32 PM To: Marcum, Pamela A Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT As long as we do not need certification, licensure and minium education requirements we will not be recognized as Laboratory Professionals. From: "Marcum, Pamela A" To: "'joelle weaver'" , "'Emily Sours'" , "histonet@lists.utsouthwestern.edu" Date: 09/10/2013 01:12 PM Subject: RE: [Histonet] Unregistered HT Sent by: histonet-bounces@lists.utsouthwestern.edu I agree we have huge gray areas and not all histology schools are as good as they could be for what we are facing in Histology. I keep harping on the fact that until we are recognized as Laboratory Professionals we will stay in this limbo. The rules determining complex testing should be revisited to what is done in Histology Laboratories today and not what we did 30 or more years ago. The Clinical Laboratory is now so automated it is hard to find anyone in most areas who can even remember doing any manual testing. The Micro lab is the closest to being as manual as areas of Histology. I am in a small market and finding a registered Histologist is harder for us. I would love to have 8 to choose from and interview. Pam Marcum From: joelle weaver [mailto:joelleweaver@hotmail.com] Sent: Tuesday, September 10, 2013 2:59 PM To: Marcum, Pamela A; 'Emily Sours'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT Well I am mostly clinical...but I think that organizations can set standards outside and beyond what CAP,CLIA etc stipulate. For the position I have now, I had to submit all my transcripts from high school up through masters in addition to proof of my ASCP certification, IHC qualification, continuing education, and professional association activity. There is a lot of gray area out there. They seem to have not had trouble getting applicants though ( and I know this varies by market), there were over 8 candidates for an HT opening, which I thought was a pretty good turn out. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: PAMarcum@uams.edu > To: talulahgosh@gmail.com; joelleweaver@hotmail.com > CC: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Unregistered HT > Date: Tue, 10 Sep 2013 13:53:26 +0000 > > Research is a different area and not controlled by CAP, CLIA and other hospital licensing or accreditation organizations. We are bound by the rules of these organizations and while I agree with you to a point. We do need minimums for training and registration by recognized licensing bodies when patient tissue is being processed for histological examination. I am sure no one thinks of this often however; there are medical legal issues with insurance we have that do not apply for research. It is also clear that registration does not mean we don't have registered people who are not as good as they should be for excellent patient care. > > I have worked in research and while I would not ever say the hiring of non-registered people is a problem for research. It is often a specialty area that requires knowing more than routine Histology. I have done plastics in research that could not ever be used in routine Histology due to the time factors and in some cases limited use with staining applications, especially IHC for some procedures. Many other areas in research require more specialized training than would be used in a routine area. I would also add some really great techs are in many phases of research. I know MT who work in Histology and are not registered as the MT BS, overrides the HT requirement for many institutions. > > Many factors must be considered for both research and routine > Histology that cover far more than just hiring registered people in certain areas of the laboratory. > > Pam Marcum > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu< mailto:histonet-bounces@lists.utsouthwestern.edu> [ mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours > Sent: Tuesday, September 10, 2013 8:20 AM > To: joelle weaver > Cc: histonet@lists.utsouthwestern.edu< mailto:histonet@lists.utsouthwestern.edu> > Subject: Re: [Histonet] Unregistered HT > > Do employers consider lab techs to be proficient enough? I've been > doing ISH and immuno for 13 years, but I'm not certified as I do research. Maybe there aren't a lot of lab techs out there? Just wondering since you might be missing someone awesome by hiring only certified people. > > "By bitching and bitching and bitching, they could exhaust the drama > of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." > > -Chuck Palahniuk, "Haunted" > > > On Mon, Sep 9, 2013 at 6:49 PM, joelle weaver > >wrote: > > > All I have is a "histology assistant" description I put together. It > > is mostly clerical, instrument up keep and other duties. My employer > > does not hire uncertified techs- due to CLIA license, grossing, FISH > > & molecular duties ( high complexity). I hope to be a clinical site > > eventually, and then they will have to complete their certification > > within one year -if they are hired on. > > If you think that will help, I can send it on. > > > > > > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > > > Date: Mon, 9 Sep 2013 15:39:12 -0500 > > > From: delsuec@gmail.com > > > To: histonet@lists.utsouthwestern.edu< mailto:histonet@lists.utsouthwestern.edu> > > > Subject: [Histonet] Unregistered HT > > > > > > Hi all, > > > > > > I'm wondering if anyone can share a job description for an > > > unregistered > > HT > > > position. I also need to know if unregistered HT's are allowed to > > > cut frozen sections. I know there is a limit to what they can do, > > > especially where IHC and special staining are concerned, but I > > > really don't know > > about > > > frozens, since it's a lot like regular microtomy, except for the > > > H&E staining of the slide. I also understand that facilities might > > > have different descriptions, but what I'm really looking for is > > > what CAP > > allows > > > them to do. > > > > > > Thanks for your help, > > > > > > Deloris Carter, HT(ASCP) > > > SMMC > > > Shawnee, KS > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu< mailto:Histonet@lists.utsouthwestern.edu> > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu< mailto:Histonet@lists.utsouthwestern.edu> > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu< mailto:Histonet@lists.utsouthwestern.edu> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ---------------------------------------------------------------------- > Confidentiality Notice: This e-mail message, including any > attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From Ronald.Houston <@t> nationwidechildrens.org Wed Sep 11 09:51:59 2013 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Wed Sep 11 09:52:05 2013 Subject: [Histonet] DNA ploidy Message-ID: Would anyone doing DNA ploidy be willing to share their protocol? It's a long time since I was involved in this and I'm sure there have been many advances Thanks Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org From jqb7 <@t> cdc.gov Wed Sep 11 09:54:29 2013 From: jqb7 <@t> cdc.gov (Sanders, Jeanine (CDC/OID/NCEZID)) Date: Wed Sep 11 09:54:50 2013 Subject: [Histonet] Unregistered HT In-Reply-To: <41D3A1AF6FEF0643BDC89E0516A6EA32D3158578@Mail2Node2.ad.uams.edu> References: , , , <41D3A1AF6FEF0643BDC89E0516A6EA32D31581E1@Mail2Node2.ad.uams.edu> <41D3A1AF6FEF0643BDC89E0516A6EA32D3158371@Mail2Node2.ad.uams.edu> <25A4DE08332B19499904459F00AAACB719C704FD68@EVS1.archildrens.org> <16F90B93CA23D446980B3D591FD02DAD08A835@UWHC-MBX14.uwhis.hosp.wisc.edu> <41D3A1AF6FEF0643BDC89E0516A6EA32D3158578@Mail2Node2.ad.uams.edu> Message-ID: Agreed. Vancouver was especially difficult due to it being outside the U.S. as well as more expensive than most other sites. The fact that the meeting crossed from Sept. into Oct. was impossible for most government employees who have a fiscal year ending Sept. This would have required 2 sets of travel vouchers, etc. and if a Continuing Resolution was not reached on Sept. 30 I would have been flown back immediately. I remember being told many years ago that NSH was scheduling the meetings in smaller host towns to contain costs. I love that idea so I hope we can keep on that track. Vancouver was the first meeting I missed in several years. Jeanine H. Sanders Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 404-639-3590 jqb7@cdc.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marcum, Pamela A Sent: Wednesday, September 11, 2013 10:46 AM To: 'Mitchell Jean A'; Horn, Hazel V; 'Weems, Joyce K.'; 'Jennifer MacDonald' Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT Agreed and now that the registries for HT and HTL require credits to remain registered it does help with keeping people in the area learning and growing. The point that other than the front office for NSH is a well taken point and the rest are volunteers. I am not always sure the front office understands hospitals, private labs, universities etc. are not sending anyone to meetings or really encouraging further education for most lab staffs. Histology here can get registration paid for and everything else is on the person attending for transportation/room and board and all other expenses we are not able to go to meetings in far flung places. Pam Marcum -----Original Message----- From: Mitchell Jean A [mailto:JMitchell@uwhealth.org] Sent: Wednesday, September 11, 2013 9:26 AM To: Horn, Hazel V; 'Weems, Joyce K.'; 'Jennifer MacDonald'; Marcum, Pamela A Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT Even if a facility does not require that techs be HT registered - the technicians themselves need go that extra step, take the initiative themselves and become HT certified. If a portion of histologists don't recognize or care about the merit of certification how can we progress our profession to the status it deserves? You have to believe in yourself in order for others to follow suit. With the exclusion of the front office; NSH is a volunteer organization that fights for our profession and it can be difficult to take on the big dogs of ASCP/CAP/CLIA. I like to see this passion for our profession and if we keep this up eventually we will see results and the professional status we deserve. Jean Mitchell, BS HT (ASCP) University of Wisconsin Hospital & Clinics Neuromuscular Laboratory 600 Highland Avenue Madison, WI 53792-5132 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Wednesday, September 11, 2013 8:30 AM To: 'Weems, Joyce K.'; 'Jennifer MacDonald'; Marcum, Pamela A Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT Histology does not get the respect or the recognition because histologists do not report results. All of the complex testing we do is overlooked because the pathologists report the results. CLIA standards are based on result reporting. The CAP has looked the other way for years because pathologists would hire unregistered techs. If pathologists would demand only registered techs half our battle would be won. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Wednesday, September 11, 2013 8:08 AM To: 'Jennifer MacDonald'; Marcum, Pamela A Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT And the reason so many have been fighting for this for years. If a lab were looking for a Medical Technologist there would be no question. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Tuesday, September 10, 2013 7:32 PM To: Marcum, Pamela A Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT As long as we do not need certification, licensure and minium education requirements we will not be recognized as Laboratory Professionals. From: "Marcum, Pamela A" To: "'joelle weaver'" , "'Emily Sours'" , "histonet@lists.utsouthwestern.edu" Date: 09/10/2013 01:12 PM Subject: RE: [Histonet] Unregistered HT Sent by: histonet-bounces@lists.utsouthwestern.edu I agree we have huge gray areas and not all histology schools are as good as they could be for what we are facing in Histology. I keep harping on the fact that until we are recognized as Laboratory Professionals we will stay in this limbo. The rules determining complex testing should be revisited to what is done in Histology Laboratories today and not what we did 30 or more years ago. The Clinical Laboratory is now so automated it is hard to find anyone in most areas who can even remember doing any manual testing. The Micro lab is the closest to being as manual as areas of Histology. I am in a small market and finding a registered Histologist is harder for us. I would love to have 8 to choose from and interview. Pam Marcum From: joelle weaver [mailto:joelleweaver@hotmail.com] Sent: Tuesday, September 10, 2013 2:59 PM To: Marcum, Pamela A; 'Emily Sours'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT Well I am mostly clinical...but I think that organizations can set standards outside and beyond what CAP,CLIA etc stipulate. For the position I have now, I had to submit all my transcripts from high school up through masters in addition to proof of my ASCP certification, IHC qualification, continuing education, and professional association activity. There is a lot of gray area out there. They seem to have not had trouble getting applicants though ( and I know this varies by market), there were over 8 candidates for an HT opening, which I thought was a pretty good turn out. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: PAMarcum@uams.edu > To: talulahgosh@gmail.com; joelleweaver@hotmail.com > CC: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Unregistered HT > Date: Tue, 10 Sep 2013 13:53:26 +0000 > > Research is a different area and not controlled by CAP, CLIA and other hospital licensing or accreditation organizations. We are bound by the rules of these organizations and while I agree with you to a point. We do need minimums for training and registration by recognized licensing bodies when patient tissue is being processed for histological examination. I am sure no one thinks of this often however; there are medical legal issues with insurance we have that do not apply for research. It is also clear that registration does not mean we don't have registered people who are not as good as they should be for excellent patient care. > > I have worked in research and while I would not ever say the hiring of non-registered people is a problem for research. It is often a specialty area that requires knowing more than routine Histology. I have done plastics in research that could not ever be used in routine Histology due to the time factors and in some cases limited use with staining applications, especially IHC for some procedures. Many other areas in research require more specialized training than would be used in a routine area. I would also add some really great techs are in many phases of research. I know MT who work in Histology and are not registered as the MT BS, overrides the HT requirement for many institutions. > > Many factors must be considered for both research and routine > Histology that cover far more than just hiring registered people in certain areas of the laboratory. > > Pam Marcum > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu< mailto:histonet-bounces@lists.utsouthwestern.edu> [ mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours > Sent: Tuesday, September 10, 2013 8:20 AM > To: joelle weaver > Cc: histonet@lists.utsouthwestern.edu< mailto:histonet@lists.utsouthwestern.edu> > Subject: Re: [Histonet] Unregistered HT > > Do employers consider lab techs to be proficient enough? I've been > doing ISH and immuno for 13 years, but I'm not certified as I do research. Maybe there aren't a lot of lab techs out there? Just wondering since you might be missing someone awesome by hiring only certified people. > > "By bitching and bitching and bitching, they could exhaust the drama > of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." > > -Chuck Palahniuk, "Haunted" > > > On Mon, Sep 9, 2013 at 6:49 PM, joelle weaver > >wrote: > > > All I have is a "histology assistant" description I put together. It > > is mostly clerical, instrument up keep and other duties. My employer > > does not hire uncertified techs- due to CLIA license, grossing, FISH > > & molecular duties ( high complexity). I hope to be a clinical site > > eventually, and then they will have to complete their certification > > within one year -if they are hired on. > > If you think that will help, I can send it on. > > > > > > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > > > Date: Mon, 9 Sep 2013 15:39:12 -0500 > > > From: delsuec@gmail.com > > > To: histonet@lists.utsouthwestern.edu< mailto:histonet@lists.utsouthwestern.edu> > > > Subject: [Histonet] Unregistered HT > > > > > > Hi all, > > > > > > I'm wondering if anyone can share a job description for an > > > unregistered > > HT > > > position. I also need to know if unregistered HT's are allowed to > > > cut frozen sections. I know there is a limit to what they can do, > > > especially where IHC and special staining are concerned, but I > > > really don't know > > about > > > frozens, since it's a lot like regular microtomy, except for the > > > H&E staining of the slide. I also understand that facilities might > > > have different descriptions, but what I'm really looking for is > > > what CAP > > allows > > > them to do. > > > > > > Thanks for your help, > > > > > > Deloris Carter, HT(ASCP) > > > SMMC > > > Shawnee, KS > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu< mailto:Histonet@lists.utsouthwestern.edu> > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu< mailto:Histonet@lists.utsouthwestern.edu> > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu< mailto:Histonet@lists.utsouthwestern.edu> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ---------------------------------------------------------------------- > Confidentiality Notice: This e-mail message, including any > attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From talulahgosh <@t> gmail.com Wed Sep 11 09:57:20 2013 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Wed Sep 11 09:57:25 2013 Subject: Fwd: [Histonet] Unregistered HT In-Reply-To: References: <41D3A1AF6FEF0643BDC89E0516A6EA32D31581E1@Mail2Node2.ad.uams.edu> <41D3A1AF6FEF0643BDC89E0516A6EA32D3158371@Mail2Node2.ad.uams.edu> <25A4DE08332B19499904459F00AAACB719C704FD68@EVS1.archildrens.org> <16F90B93CA23D446980B3D591FD02DAD08A835@UWHC-MBX14.uwhis.hosp.wisc.edu> <41D3A1AF6FEF0643BDC89E0516A6EA32D3158578@Mail2Node2.ad.uams.edu> Message-ID: How do you gain credits? Do you get them for attending seminars or do you have to take actual courses? Are these the CME's I always see noted for lectures? I never knew what those were for. Emily "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." -Chuck Palahniuk, "Haunted" On Wed, Sep 11, 2013 at 10:46 AM, Marcum, Pamela A wrote: > Agreed and now that the registries for HT and HTL require credits to > remain registered it does help with keeping people in the area learning and > growing. The point that other than the front office for NSH is a well > taken point and the rest are volunteers. I am not always sure the front > office understands hospitals, private labs, universities etc. are not > sending anyone to meetings or really encouraging further education for most > lab staffs. Histology here can get registration paid for and everything > else is on the person attending for transportation/room and board and all > other expenses we are not able to go to meetings in far flung places. > > Pam Marcum > > > From lpwenk <@t> sbcglobal.net Wed Sep 11 10:01:10 2013 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Wed Sep 11 10:01:24 2013 Subject: [Histonet] Unregistered HT In-Reply-To: <41D3A1AF6FEF0643BDC89E0516A6EA32D31584FA@Mail2Node2.ad.uams.edu> References: , , , <41D3A1AF6FEF0643BDC89E0516A6EA32D31581E1@Mail2Node2.ad.uams.edu><41D3A1AF6FEF0643BDC89E0516A6EA32D3158371@Mail2Node2.ad.uams.edu> <41D3A1AF6FEF0643BDC89E0516A6EA32D31584FA@Mail2Node2.ad.uams.edu> Message-ID: Just a comment on the comment about there being "not enough" HT/HTL programs in the US. Agreed. There are 38 HT and 7 HTL, for a total of 45. Compare that with 223 MLS/MT and 233 MLT, for a total of 456, and we histotechs have 1/10 the number of programs as med techs. If you are interested in starting a HT or HTL program in your area, either in a hospital or a college, or would like information about what it would take to start one, Sarah Bajer and I are presenting at Providence, RI, Workshop #2 on How to Start a HT or HTL School, on Saturday Sept. 21, from 8:00 am - 11:30 am. Come and collect the information. To register, go to the NSH website http://www.histoconvention.org/ Peggy A. Wenk, HTL(ASCP)SLS -----Original Message----- From: Marcum, Pamela A Sent: Wednesday, September 11, 2013 9:18 AM To: 'Weems, Joyce K.' ; 'JenniferMacDonald' Cc: histonet@lists.utsouthwestern.edu ; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT We have heard for years that NSH is working on changing the status to Laboratory Professionals and so far as I have heard nothing happening to make the change. A large part of this is ASCP/all organizations inspecting/licensing are still looking at us as non-skilled labor that requires only OJT training or an AA/AS degree at best. We have a few one year schools and some online however; not enough to cover the need for HTs and HTLs. We need ASCP/CAP/CLIA/all organizations that inspect us and tell us how to run the laboratories to actually understand we have changed in the last 25 years and no longer just cut and do H&Es. I am not sure where to go as I have been bringing this up for years and see no movement. Take the test to be an inspector for CAP and you will see the problem clearly. It is 98% Clinical Laboratory and maybe 2% Anatomic Pathology. If you don't know Clinical you will have a problem passing the test without help. Pam Marcum -----Original Message----- From: Weems, Joyce K. [mailto:Joyce.Weems@emoryhealthcare.org] Sent: Wednesday, September 11, 2013 8:08 AM To: 'Jennifer MacDonald'; Marcum, Pamela A Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT And the reason so many have been fighting for this for years. If a lab were looking for a Medical Technologist there would be no question. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Tuesday, September 10, 2013 7:32 PM To: Marcum, Pamela A Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT As long as we do not need certification, licensure and minium education requirements we will not be recognized as Laboratory Professionals. From: "Marcum, Pamela A" To: "'joelle weaver'" , "'Emily Sours'" , "histonet@lists.utsouthwestern.edu" Date: 09/10/2013 01:12 PM Subject: RE: [Histonet] Unregistered HT Sent by: histonet-bounces@lists.utsouthwestern.edu I agree we have huge gray areas and not all histology schools are as good as they could be for what we are facing in Histology. I keep harping on the fact that until we are recognized as Laboratory Professionals we will stay in this limbo. The rules determining complex testing should be revisited to what is done in Histology Laboratories today and not what we did 30 or more years ago. The Clinical Laboratory is now so automated it is hard to find anyone in most areas who can even remember doing any manual testing. The Micro lab is the closest to being as manual as areas of Histology. I am in a small market and finding a registered Histologist is harder for us. I would love to have 8 to choose from and interview. Pam Marcum From: joelle weaver [mailto:joelleweaver@hotmail.com] Sent: Tuesday, September 10, 2013 2:59 PM To: Marcum, Pamela A; 'Emily Sours'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT Well I am mostly clinical...but I think that organizations can set standards outside and beyond what CAP,CLIA etc stipulate. For the position I have now, I had to submit all my transcripts from high school up through masters in addition to proof of my ASCP certification, IHC qualification, continuing education, and professional association activity. There is a lot of gray area out there. They seem to have not had trouble getting applicants though ( and I know this varies by market), there were over 8 candidates for an HT opening, which I thought was a pretty good turn out. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: PAMarcum@uams.edu > To: talulahgosh@gmail.com; joelleweaver@hotmail.com > CC: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Unregistered HT > Date: Tue, 10 Sep 2013 13:53:26 +0000 > > Research is a different area and not controlled by CAP, CLIA and other hospital licensing or accreditation organizations. We are bound by the rules of these organizations and while I agree with you to a point. We do need minimums for training and registration by recognized licensing bodies when patient tissue is being processed for histological examination. I am sure no one thinks of this often however; there are medical legal issues with insurance we have that do not apply for research. It is also clear that registration does not mean we don't have registered people who are not as good as they should be for excellent patient care. > > I have worked in research and while I would not ever say the hiring of non-registered people is a problem for research. It is often a specialty area that requires knowing more than routine Histology. I have done plastics in research that could not ever be used in routine Histology due to the time factors and in some cases limited use with staining applications, especially IHC for some procedures. Many other areas in research require more specialized training than would be used in a routine area. I would also add some really great techs are in many phases of research. I know MT who work in Histology and are not registered as the MT BS, overrides the HT requirement for many institutions. > > Many factors must be considered for both research and routine > Histology that cover far more than just hiring registered people in certain areas of the laboratory. > > Pam Marcum > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu< mailto:histonet-bounces@lists.utsouthwestern.edu> [ mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours > Sent: Tuesday, September 10, 2013 8:20 AM > To: joelle weaver > Cc: histonet@lists.utsouthwestern.edu< mailto:histonet@lists.utsouthwestern.edu> > Subject: Re: [Histonet] Unregistered HT > > Do employers consider lab techs to be proficient enough? I've been > doing ISH and immuno for 13 years, but I'm not certified as I do research. Maybe there aren't a lot of lab techs out there? Just wondering since you might be missing someone awesome by hiring only certified people. > > "By bitching and bitching and bitching, they could exhaust the drama > of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." > > -Chuck Palahniuk, "Haunted" > > > On Mon, Sep 9, 2013 at 6:49 PM, joelle weaver > >wrote: > > > All I have is a "histology assistant" description I put together. It > > is mostly clerical, instrument up keep and other duties. My employer > > does not hire uncertified techs- due to CLIA license, grossing, FISH > > & molecular duties ( high complexity). I hope to be a clinical site > > eventually, and then they will have to complete their certification > > within one year -if they are hired on. > > If you think that will help, I can send it on. > > > > > > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > > > Date: Mon, 9 Sep 2013 15:39:12 -0500 > > > From: delsuec@gmail.com > > > To: histonet@lists.utsouthwestern.edu< mailto:histonet@lists.utsouthwestern.edu> > > > Subject: [Histonet] Unregistered HT > > > > > > Hi all, > > > > > > I'm wondering if anyone can share a job description for an > > > unregistered > > HT > > > position. I also need to know if unregistered HT's are allowed to > > > cut frozen sections. I know there is a limit to what they can do, > > > especially where IHC and special staining are concerned, but I > > > really don't know > > about > > > frozens, since it's a lot like regular microtomy, except for the > > > H&E staining of the slide. I also understand that facilities might > > > have different descriptions, but what I'm really looking for is > > > what CAP > > allows > > > them to do. > > > > > > Thanks for your help, > > > > > > Deloris Carter, HT(ASCP) > > > SMMC > > > Shawnee, KS > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu< mailto:Histonet@lists.utsouthwestern.edu> > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu< mailto:Histonet@lists.utsouthwestern.edu> > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu< mailto:Histonet@lists.utsouthwestern.edu> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ---------------------------------------------------------------------- > Confidentiality Notice: This e-mail message, including any > attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 <@t> earthlink.net Wed Sep 11 10:09:04 2013 From: relia1 <@t> earthlink.net (Pam Barker) Date: Wed Sep 11 10:09:14 2013 Subject: [Histonet] Unregistered HT In-Reply-To: References: , , , <41D3A1AF6FEF0643BDC89E0516A6EA32D31581E1@Mail2Node2.ad.uams.edu><41D3A1AF6FEF0643BDC89E0516A6EA32D3158371@Mail2Node2.ad.uams.edu> <41D3A1AF6FEF0643BDC89E0516A6EA32D31584FA@Mail2Node2.ad.uams.edu> Message-ID: <00e501ceaf00$dcf222c0$96d66840$@earthlink.net> My 2 cents... So the real "shortage" in the field is ASCP HT/HTL certified techs with 2+ years of experience who are CLIA qualified to gross. Just saying... I know a great many uncertified experienced techs and even more entry level ASCP certified recent graduates of histology schools who are desperately looking for positions and willing to relocate anywhere and work any shift... Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker ? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk Sent: Wednesday, September 11, 2013 11:01 AM To: Marcum, Pamela A; 'Weems, Joyce K.'; 'JenniferMacDonald' Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Unregistered HT Just a comment on the comment about there being "not enough" HT/HTL programs in the US. Agreed. There are 38 HT and 7 HTL, for a total of 45. Compare that with 223 MLS/MT and 233 MLT, for a total of 456, and we histotechs have 1/10 the number of programs as med techs. If you are interested in starting a HT or HTL program in your area, either in a hospital or a college, or would like information about what it would take to start one, Sarah Bajer and I are presenting at Providence, RI, Workshop #2 on How to Start a HT or HTL School, on Saturday Sept. 21, from 8:00 am - 11:30 am. Come and collect the information. To register, go to the NSH website http://www.histoconvention.org/ Peggy A. Wenk, HTL(ASCP)SLS -----Original Message----- From: Marcum, Pamela A Sent: Wednesday, September 11, 2013 9:18 AM To: 'Weems, Joyce K.' ; 'JenniferMacDonald' Cc: histonet@lists.utsouthwestern.edu ; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT We have heard for years that NSH is working on changing the status to Laboratory Professionals and so far as I have heard nothing happening to make the change. A large part of this is ASCP/all organizations inspecting/licensing are still looking at us as non-skilled labor that requires only OJT training or an AA/AS degree at best. We have a few one year schools and some online however; not enough to cover the need for HTs and HTLs. We need ASCP/CAP/CLIA/all organizations that inspect us and tell us how to run the laboratories to actually understand we have changed in the last 25 years and no longer just cut and do H&Es. I am not sure where to go as I have been bringing this up for years and see no movement. Take the test to be an inspector for CAP and you will see the problem clearly. It is 98% Clinical Laboratory and maybe 2% Anatomic Pathology. If you don't know Clinical you will have a problem passing the test without help. Pam Marcum -----Original Message----- From: Weems, Joyce K. [mailto:Joyce.Weems@emoryhealthcare.org] Sent: Wednesday, September 11, 2013 8:08 AM To: 'Jennifer MacDonald'; Marcum, Pamela A Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT And the reason so many have been fighting for this for years. If a lab were looking for a Medical Technologist there would be no question. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Tuesday, September 10, 2013 7:32 PM To: Marcum, Pamela A Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT As long as we do not need certification, licensure and minium education requirements we will not be recognized as Laboratory Professionals. From: "Marcum, Pamela A" To: "'joelle weaver'" , "'Emily Sours'" , "histonet@lists.utsouthwestern.edu" Date: 09/10/2013 01:12 PM Subject: RE: [Histonet] Unregistered HT Sent by: histonet-bounces@lists.utsouthwestern.edu I agree we have huge gray areas and not all histology schools are as good as they could be for what we are facing in Histology. I keep harping on the fact that until we are recognized as Laboratory Professionals we will stay in this limbo. The rules determining complex testing should be revisited to what is done in Histology Laboratories today and not what we did 30 or more years ago. The Clinical Laboratory is now so automated it is hard to find anyone in most areas who can even remember doing any manual testing. The Micro lab is the closest to being as manual as areas of Histology. I am in a small market and finding a registered Histologist is harder for us. I would love to have 8 to choose from and interview. Pam Marcum From: joelle weaver [mailto:joelleweaver@hotmail.com] Sent: Tuesday, September 10, 2013 2:59 PM To: Marcum, Pamela A; 'Emily Sours'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT Well I am mostly clinical...but I think that organizations can set standards outside and beyond what CAP,CLIA etc stipulate. For the position I have now, I had to submit all my transcripts from high school up through masters in addition to proof of my ASCP certification, IHC qualification, continuing education, and professional association activity. There is a lot of gray area out there. They seem to have not had trouble getting applicants though ( and I know this varies by market), there were over 8 candidates for an HT opening, which I thought was a pretty good turn out. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: PAMarcum@uams.edu > To: talulahgosh@gmail.com; joelleweaver@hotmail.com > CC: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Unregistered HT > Date: Tue, 10 Sep 2013 13:53:26 +0000 > > Research is a different area and not controlled by CAP, CLIA and other hospital licensing or accreditation organizations. We are bound by the rules of these organizations and while I agree with you to a point. We do need minimums for training and registration by recognized licensing bodies when patient tissue is being processed for histological examination. I am sure no one thinks of this often however; there are medical legal issues with insurance we have that do not apply for research. It is also clear that registration does not mean we don't have registered people who are not as good as they should be for excellent patient care. > > I have worked in research and while I would not ever say the hiring of non-registered people is a problem for research. It is often a specialty area that requires knowing more than routine Histology. I have done plastics in research that could not ever be used in routine Histology due to the time factors and in some cases limited use with staining applications, especially IHC for some procedures. Many other areas in research require more specialized training than would be used in a routine area. I would also add some really great techs are in many phases of research. I know MT who work in Histology and are not registered as the MT BS, overrides the HT requirement for many institutions. > > Many factors must be considered for both research and routine > Histology that cover far more than just hiring registered people in certain areas of the laboratory. > > Pam Marcum > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu< mailto:histonet-bounces@lists.utsouthwestern.edu> [ mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours > Sent: Tuesday, September 10, 2013 8:20 AM > To: joelle weaver > Cc: histonet@lists.utsouthwestern.edu< mailto:histonet@lists.utsouthwestern.edu> > Subject: Re: [Histonet] Unregistered HT > > Do employers consider lab techs to be proficient enough? I've been > doing ISH and immuno for 13 years, but I'm not certified as I do research. Maybe there aren't a lot of lab techs out there? Just wondering since you might be missing someone awesome by hiring only certified people. > > "By bitching and bitching and bitching, they could exhaust the drama > of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." > > -Chuck Palahniuk, "Haunted" > > > On Mon, Sep 9, 2013 at 6:49 PM, joelle weaver > >wrote: > > > All I have is a "histology assistant" description I put together. It > > is mostly clerical, instrument up keep and other duties. My employer > > does not hire uncertified techs- due to CLIA license, grossing, FISH > > & molecular duties ( high complexity). I hope to be a clinical site > > eventually, and then they will have to complete their certification > > within one year -if they are hired on. > > If you think that will help, I can send it on. > > > > > > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > > > Date: Mon, 9 Sep 2013 15:39:12 -0500 > > > From: delsuec@gmail.com > > > To: histonet@lists.utsouthwestern.edu< mailto:histonet@lists.utsouthwestern.edu> > > > Subject: [Histonet] Unregistered HT > > > > > > Hi all, > > > > > > I'm wondering if anyone can share a job description for an > > > unregistered > > HT > > > position. I also need to know if unregistered HT's are allowed to > > > cut frozen sections. I know there is a limit to what they can do, > > > especially where IHC and special staining are concerned, but I > > > really don't know > > about > > > frozens, since it's a lot like regular microtomy, except for the > > > H&E staining of the slide. I also understand that facilities might > > > have different descriptions, but what I'm really looking for is > > > what CAP > > allows > > > them to do. > > > > > > Thanks for your help, > > > > > > Deloris Carter, HT(ASCP) > > > SMMC > > > Shawnee, KS > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu< mailto:Histonet@lists.utsouthwestern.edu> > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu< mailto:Histonet@lists.utsouthwestern.edu> > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu< mailto:Histonet@lists.utsouthwestern.edu> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ---------------------------------------------------------------------- > Confidentiality Notice: This e-mail message, including any > attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Wed Sep 11 10:10:08 2013 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Wed Sep 11 10:10:17 2013 Subject: [Histonet] Unregistered HT In-Reply-To: References: , , , , , , <41D3A1AF6FEF0643BDC89E0516A6EA32D31581E1@Mail2Node2.ad.uams.edu><41D3A1AF6FEF0643BDC89E0516A6EA32D3158371@Mail2Node2.ad.uams.edu>, <41D3A1AF6FEF0643BDC89E0516A6EA32D31584FA@Mail2Node2.ad.uams.edu>, Message-ID: Peggy I am not able to attend the workshop since I am alone right now setting up the lab. Would it be possible to collect or provide some way that those interested but not able to attend, that we might be able to view or purchase the content of the workshop on starting a program following the presentation? I have been through the NAACLS process and their training in Chicago, but there is so much to know about this topic, and I would like to take advantage of the information. We definitely need more programs for histotechnology. I want to get going on being a site next year ( once the lab is up & running) for programs a state or so away- and then maybe get involved with a program or getting one going after that so this information might really be valuable for me. Thanks Joelle Joelle Weaver MAOM, HTL (ASCP) QIHC > From: lpwenk@sbcglobal.net > To: PAMarcum@uams.edu; Joyce.Weems@emoryhealthcare.org; JMacDonald@mtsac.edu > Date: Wed, 11 Sep 2013 11:01:10 -0400 > Subject: Re: [Histonet] Unregistered HT > CC: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu > > Just a comment on the comment about there being "not enough" HT/HTL programs > in the US. Agreed. There are 38 HT and 7 HTL, for a total of 45. Compare > that with 223 MLS/MT and 233 MLT, for a total of 456, and we histotechs have > 1/10 the number of programs as med techs. > > If you are interested in starting a HT or HTL program in your area, either > in a hospital or a college, or would like information about what it would > take to start one, Sarah Bajer and I are presenting at Providence, RI, > Workshop #2 on How to Start a HT or HTL School, on Saturday Sept. 21, from > 8:00 am - 11:30 am. > > Come and collect the information. To register, go to the NSH website > http://www.histoconvention.org/ > > Peggy A. Wenk, HTL(ASCP)SLS > > -----Original Message----- > From: Marcum, Pamela A > Sent: Wednesday, September 11, 2013 9:18 AM > To: 'Weems, Joyce K.' ; 'JenniferMacDonald' > Cc: histonet@lists.utsouthwestern.edu ; > histonet-bounces@lists.utsouthwestern.edu > Subject: RE: [Histonet] Unregistered HT > > We have heard for years that NSH is working on changing the status to > Laboratory Professionals and so far as I have heard nothing happening to > make the change. A large part of this is ASCP/all organizations > inspecting/licensing are still looking at us as non-skilled labor that > requires only OJT training or an AA/AS degree at best. We have a few one > year schools and some online however; not enough to cover the need for HTs > and HTLs. > > We need ASCP/CAP/CLIA/all organizations that inspect us and tell us how to > run the laboratories to actually understand we have changed in the last 25 > years and no longer just cut and do H&Es. I am not sure where to go as I > have been bringing this up for years and see no movement. Take the test to > be an inspector for CAP and you will see the problem clearly. It is 98% > Clinical Laboratory and maybe 2% Anatomic Pathology. If you don't know > Clinical you will have a problem passing the test without help. > > Pam Marcum > > -----Original Message----- > From: Weems, Joyce K. [mailto:Joyce.Weems@emoryhealthcare.org] > Sent: Wednesday, September 11, 2013 8:08 AM > To: 'Jennifer MacDonald'; Marcum, Pamela A > Cc: histonet@lists.utsouthwestern.edu; > histonet-bounces@lists.utsouthwestern.edu > Subject: RE: [Histonet] Unregistered HT > > And the reason so many have been fighting for this for years. If a lab were > looking for a Medical Technologist there would be no question. > > > Joyce Weems > Pathology Manager > 678-843-7376 Phone > 678-843-7831 Fax > joyce.weems@emoryhealthcare.org > > > > www.saintjosephsatlanta.org > 5665 Peachtree Dunwoody Road > Atlanta, GA 30342 > > This e-mail, including any attachments is the property of Saint Joseph's > Hospital and is intended for the sole use of the intended recipient(s). It > may contain information that is privileged and confidential. Any > unauthorized review, use, disclosure, or distribution is prohibited. If you > are not the intended recipient, please delete this message, and reply to the > sender regarding the error in a separate email. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer > MacDonald > Sent: Tuesday, September 10, 2013 7:32 PM > To: Marcum, Pamela A > Cc: histonet@lists.utsouthwestern.edu; > histonet-bounces@lists.utsouthwestern.edu > Subject: RE: [Histonet] Unregistered HT > > As long as we do not need certification, licensure and minium education > requirements we will not be recognized as Laboratory Professionals. > > > > From: "Marcum, Pamela A" > To: "'joelle weaver'" , "'Emily Sours'" > , "histonet@lists.utsouthwestern.edu" > > Date: 09/10/2013 01:12 PM > Subject: RE: [Histonet] Unregistered HT > Sent by: histonet-bounces@lists.utsouthwestern.edu > > > > I agree we have huge gray areas and not all histology schools are as good as > they could be for what we are facing in Histology. I keep harping on the > fact that until we are recognized as Laboratory Professionals we will stay > in this limbo. The rules determining complex testing should be revisited to > what is done in Histology Laboratories today and not what we did 30 or more > years ago. The Clinical Laboratory is now so automated it is hard to find > anyone in most areas who can even remember doing any manual testing. The > Micro lab is the closest to being as manual as areas of Histology. > > I am in a small market and finding a registered Histologist is harder for > us. I would love to have 8 to choose from and interview. > > Pam Marcum > > From: joelle weaver [mailto:joelleweaver@hotmail.com] > Sent: Tuesday, September 10, 2013 2:59 PM > To: Marcum, Pamela A; 'Emily Sours'; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Unregistered HT > > Well I am mostly clinical...but I think that organizations can set > standards outside and beyond what CAP,CLIA etc stipulate. For the position I > have now, I had to submit all my transcripts from high school up through > masters in addition to proof of my ASCP certification, IHC qualification, > continuing education, and professional association activity. There is a lot > of gray area out there. They seem to have not had trouble getting applicants > though ( and I know this varies by market), there were over 8 candidates for > an HT opening, which I thought was a pretty good turn out. > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > From: PAMarcum@uams.edu > > To: talulahgosh@gmail.com; joelleweaver@hotmail.com > > CC: histonet@lists.utsouthwestern.edu > > Subject: RE: [Histonet] Unregistered HT > > Date: Tue, 10 Sep 2013 13:53:26 +0000 > > > > Research is a different area and not controlled by CAP, CLIA and other > hospital licensing or accreditation organizations. We are bound by the rules > of these organizations and while I agree with you to a point. We do need > minimums for training and registration by recognized licensing bodies when > patient tissue is being processed for histological examination. I am sure no > one thinks of this often however; there are medical legal issues with > insurance we have that do not apply for research. It is also clear that > registration does not mean we don't have registered people who are not as > good as they should be for excellent patient care. > > > > I have worked in research and while I would not ever say the hiring of > non-registered people is a problem for research. It is often a specialty > area that requires knowing more than routine Histology. I have done plastics > in research that could not ever be used in routine Histology due to the time > factors and in some cases limited use with staining applications, especially > IHC for some procedures. Many other areas in research require more > specialized training than would be used in a routine area. I would also add > some really great techs are in many phases of research. I know MT who work > in Histology and are not registered as the MT BS, overrides the HT > requirement for many institutions. > > > > Many factors must be considered for both research and routine > > Histology > that cover far more than just hiring registered people in certain areas of > the laboratory. > > > > Pam Marcum > > > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu< > mailto:histonet-bounces@lists.utsouthwestern.edu> [ > mailto:histonet-bounces@lists.utsouthwestern.edu] mailto:histonet-bounces@lists.utsouthwestern.edu]> On Behalf Of Emily Sours > > Sent: Tuesday, September 10, 2013 8:20 AM > > To: joelle weaver > > Cc: histonet@lists.utsouthwestern.edu< > mailto:histonet@lists.utsouthwestern.edu> > > Subject: Re: [Histonet] Unregistered HT > > > > Do employers consider lab techs to be proficient enough? I've been > > doing > ISH and immuno for 13 years, but I'm not certified as I do research. Maybe > there aren't a lot of lab techs out there? Just wondering since you might be > missing someone awesome by hiring only certified people. > > > > "By bitching and bitching and bitching, they could exhaust the drama > > of > their own horror stories. Grow bored. Only then could they accept a new > story for their lives. Move forward." > > > > -Chuck Palahniuk, "Haunted" > > > > > > On Mon, Sep 9, 2013 at 6:49 PM, joelle weaver > > mailto:joelleweaver@hotmail.com>>wrote: > > > > > All I have is a "histology assistant" description I put together. It > > > is mostly clerical, instrument up keep and other duties. My employer > > > does not hire uncertified techs- due to CLIA license, grossing, FISH > > > & molecular duties ( high complexity). I hope to be a clinical site > > > eventually, and then they will have to complete their certification > > > within one year -if they are hired on. > > > If you think that will help, I can send it on. > > > > > > > > > > > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > > > > > Date: Mon, 9 Sep 2013 15:39:12 -0500 > > > > From: delsuec@gmail.com > > > > To: histonet@lists.utsouthwestern.edu< > mailto:histonet@lists.utsouthwestern.edu> > > > > Subject: [Histonet] Unregistered HT > > > > > > > > Hi all, > > > > > > > > I'm wondering if anyone can share a job description for an > > > > unregistered > > > HT > > > > position. I also need to know if unregistered HT's are allowed to > > > > cut frozen sections. I know there is a limit to what they can do, > > > > especially where IHC and special staining are concerned, but I > > > > really don't know > > > about > > > > frozens, since it's a lot like regular microtomy, except for the > > > > H&E staining of the slide. I also understand that facilities might > > > > have different descriptions, but what I'm really looking for is > > > > what CAP > > > allows > > > > them to do. > > > > > > > > Thanks for your help, > > > > > > > > Deloris Carter, HT(ASCP) > > > > SMMC > > > > Shawnee, KS > > > > _______________________________________________ > > > > Histonet mailing list > > > > Histonet@lists.utsouthwestern.edu< > mailto:Histonet@lists.utsouthwestern.edu> > > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu< > mailto:Histonet@lists.utsouthwestern.edu> > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu< > mailto:Histonet@lists.utsouthwestern.edu> > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ---------------------------------------------------------------------- > > Confidentiality Notice: This e-mail message, including any > > attachments, > is for the sole use of the intended recipient(s) and may contain > confidential and privileged information. Any unauthorized review, use, > disclosure or distribution is prohibited. If you are not the intended > recipient, please contact the sender by reply e-mail and destroy all copies > of the original message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ________________________________ > > This e-mail message (including any attachments) is for the sole use of the > intended recipient(s) and may contain confidential and privileged > information. If the reader of this message is not the intended recipient, > you are hereby notified that any dissemination, distribution or copying of > this message (including any attachments) is strictly prohibited. > > If you have received this message in error, please contact the sender by > reply e-mail message and destroy all copies of the original message > (including attachments). > > ---------------------------------------------------------------------- > Confidentiality Notice: This e-mail message, including any attachments, is > for the sole use of the intended recipient(s) and may contain confidential > and privileged information. Any unauthorized review, use, disclosure or > distribution is prohibited. If you are not the intended recipient, please > contact the sender by reply e-mail and destroy all copies of the original > message. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Wed Sep 11 10:12:20 2013 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Wed Sep 11 10:12:29 2013 Subject: [Histonet] Unregistered HT In-Reply-To: <00e501ceaf00$dcf222c0$96d66840$@earthlink.net> References: , , , , , , <41D3A1AF6FEF0643BDC89E0516A6EA32D31581E1@Mail2Node2.ad.uams.edu><41D3A1AF6FEF0643BDC89E0516A6EA32D3158371@Mail2Node2.ad.uams.edu> <41D3A1AF6FEF0643BDC89E0516A6EA32D31584FA@Mail2Node2.ad.uams.edu>, , <00e501ceaf00$dcf222c0$96d66840$@earthlink.net> Message-ID: Thanks for your point of view and insights Pam- we need to know what employers are seeking to keep marketable. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: relia1@earthlink.net > To: lpwenk@sbcglobal.net > Date: Wed, 11 Sep 2013 11:09:04 -0400 > Subject: RE: [Histonet] Unregistered HT > CC: histonet@lists.utsouthwestern.edu > > My 2 cents... > So the real "shortage" in the field is ASCP HT/HTL certified techs with 2+ > years of experience who are CLIA qualified to gross. Just saying... I know > a great many uncertified experienced techs and even more entry level ASCP > certified recent graduates of histology schools who are desperately looking > for positions and willing to relocate anywhere and work any shift... > > Thanks-Pam > > Right Place, Right Time, Right Move with RELIA! > > Thank You! > Pam M. Barker > > Pam Barker > President/Senior Recruiting Specialist-Histology > RELIA Solutions > Specialists in Allied Healthcare Recruiting > 5703 Red Bug Lake Road #330 > Winter Springs, FL 32708-4969 > Phone: (407)657-2027 > Cell: (407)353-5070 > FAX: (407)678-2788 > E-mail: relia1@earthlink.net > www.facebook.com/PamBarkerRELIA > www.linkedin.com/in/reliasolutions > www.twitter.com/pamatrelia > > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy > Wenk > Sent: Wednesday, September 11, 2013 11:01 AM > To: Marcum, Pamela A; 'Weems, Joyce K.'; 'JenniferMacDonald' > Cc: histonet@lists.utsouthwestern.edu; > histonet-bounces@lists.utsouthwestern.edu > Subject: Re: [Histonet] Unregistered HT > > Just a comment on the comment about there being "not enough" HT/HTL programs > in the US. Agreed. There are 38 HT and 7 HTL, for a total of 45. Compare > that with 223 MLS/MT and 233 MLT, for a total of 456, and we histotechs have > 1/10 the number of programs as med techs. > > If you are interested in starting a HT or HTL program in your area, either > in a hospital or a college, or would like information about what it would > take to start one, Sarah Bajer and I are presenting at Providence, RI, > Workshop #2 on How to Start a HT or HTL School, on Saturday Sept. 21, from > 8:00 am - 11:30 am. > > Come and collect the information. To register, go to the NSH website > http://www.histoconvention.org/ > > Peggy A. Wenk, HTL(ASCP)SLS > > -----Original Message----- > From: Marcum, Pamela A > Sent: Wednesday, September 11, 2013 9:18 AM > To: 'Weems, Joyce K.' ; 'JenniferMacDonald' > Cc: histonet@lists.utsouthwestern.edu ; > histonet-bounces@lists.utsouthwestern.edu > Subject: RE: [Histonet] Unregistered HT > > We have heard for years that NSH is working on changing the status to > Laboratory Professionals and so far as I have heard nothing happening to > make the change. A large part of this is ASCP/all organizations > inspecting/licensing are still looking at us as non-skilled labor that > requires only OJT training or an AA/AS degree at best. We have a few one > year schools and some online however; not enough to cover the need for HTs > and HTLs. > > We need ASCP/CAP/CLIA/all organizations that inspect us and tell us how to > run the laboratories to actually understand we have changed in the last 25 > years and no longer just cut and do H&Es. I am not sure where to go as I > have been bringing this up for years and see no movement. Take the test to > be an inspector for CAP and you will see the problem clearly. It is 98% > Clinical Laboratory and maybe 2% Anatomic Pathology. If you don't know > Clinical you will have a problem passing the test without help. > > Pam Marcum > > -----Original Message----- > From: Weems, Joyce K. [mailto:Joyce.Weems@emoryhealthcare.org] > Sent: Wednesday, September 11, 2013 8:08 AM > To: 'Jennifer MacDonald'; Marcum, Pamela A > Cc: histonet@lists.utsouthwestern.edu; > histonet-bounces@lists.utsouthwestern.edu > Subject: RE: [Histonet] Unregistered HT > > And the reason so many have been fighting for this for years. If a lab were > looking for a Medical Technologist there would be no question. > > > Joyce Weems > Pathology Manager > 678-843-7376 Phone > 678-843-7831 Fax > joyce.weems@emoryhealthcare.org > > > > www.saintjosephsatlanta.org > 5665 Peachtree Dunwoody Road > Atlanta, GA 30342 > > This e-mail, including any attachments is the property of Saint Joseph's > Hospital and is intended for the sole use of the intended recipient(s). It > may contain information that is privileged and confidential. Any > unauthorized review, use, disclosure, or distribution is prohibited. If you > are not the intended recipient, please delete this message, and reply to the > > sender regarding the error in a separate email. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer > MacDonald > Sent: Tuesday, September 10, 2013 7:32 PM > To: Marcum, Pamela A > Cc: histonet@lists.utsouthwestern.edu; > histonet-bounces@lists.utsouthwestern.edu > Subject: RE: [Histonet] Unregistered HT > > As long as we do not need certification, licensure and minium education > requirements we will not be recognized as Laboratory Professionals. > > > > From: "Marcum, Pamela A" > To: "'joelle weaver'" , "'Emily Sours'" > , "histonet@lists.utsouthwestern.edu" > > Date: 09/10/2013 01:12 PM > Subject: RE: [Histonet] Unregistered HT > Sent by: histonet-bounces@lists.utsouthwestern.edu > > > > I agree we have huge gray areas and not all histology schools are as good as > > they could be for what we are facing in Histology. I keep harping on the > fact that until we are recognized as Laboratory Professionals we will stay > in this limbo. The rules determining complex testing should be revisited to > > what is done in Histology Laboratories today and not what we did 30 or more > years ago. The Clinical Laboratory is now so automated it is hard to find > anyone in most areas who can even remember doing any manual testing. The > Micro lab is the closest to being as manual as areas of Histology. > > I am in a small market and finding a registered Histologist is harder for > us. I would love to have 8 to choose from and interview. > > Pam Marcum > > From: joelle weaver [mailto:joelleweaver@hotmail.com] > Sent: Tuesday, September 10, 2013 2:59 PM > To: Marcum, Pamela A; 'Emily Sours'; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Unregistered HT > > Well I am mostly clinical...but I think that organizations can set > standards outside and beyond what CAP,CLIA etc stipulate. For the position I > > have now, I had to submit all my transcripts from high school up through > masters in addition to proof of my ASCP certification, IHC qualification, > continuing education, and professional association activity. There is a lot > of gray area out there. They seem to have not had trouble getting applicants > > though ( and I know this varies by market), there were over 8 candidates for > > an HT opening, which I thought was a pretty good turn out. > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > From: PAMarcum@uams.edu > > To: talulahgosh@gmail.com; joelleweaver@hotmail.com > > CC: histonet@lists.utsouthwestern.edu > > Subject: RE: [Histonet] Unregistered HT > > Date: Tue, 10 Sep 2013 13:53:26 +0000 > > > > Research is a different area and not controlled by CAP, CLIA and other > hospital licensing or accreditation organizations. We are bound by the rules > > of these organizations and while I agree with you to a point. We do need > minimums for training and registration by recognized licensing bodies when > patient tissue is being processed for histological examination. I am sure no > > one thinks of this often however; there are medical legal issues with > insurance we have that do not apply for research. It is also clear that > registration does not mean we don't have registered people who are not as > good as they should be for excellent patient care. > > > > I have worked in research and while I would not ever say the hiring of > non-registered people is a problem for research. It is often a specialty > area that requires knowing more than routine Histology. I have done plastics > > in research that could not ever be used in routine Histology due to the time > > factors and in some cases limited use with staining applications, especially > > IHC for some procedures. Many other areas in research require more > specialized training than would be used in a routine area. I would also add > some really great techs are in many phases of research. I know MT who work > in Histology and are not registered as the MT BS, overrides the HT > requirement for many institutions. > > > > Many factors must be considered for both research and routine > > Histology > that cover far more than just hiring registered people in certain areas of > the laboratory. > > > > Pam Marcum > > > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu< > mailto:histonet-bounces@lists.utsouthwestern.edu> [ > mailto:histonet-bounces@lists.utsouthwestern.edu] mailto:histonet-bounces@lists.utsouthwestern.edu]> On Behalf Of Emily Sours > > Sent: Tuesday, September 10, 2013 8:20 AM > > To: joelle weaver > > Cc: histonet@lists.utsouthwestern.edu< > mailto:histonet@lists.utsouthwestern.edu> > > Subject: Re: [Histonet] Unregistered HT > > > > Do employers consider lab techs to be proficient enough? I've been > > doing > ISH and immuno for 13 years, but I'm not certified as I do research. Maybe > there aren't a lot of lab techs out there? Just wondering since you might be > > missing someone awesome by hiring only certified people. > > > > "By bitching and bitching and bitching, they could exhaust the drama > > of > their own horror stories. Grow bored. Only then could they accept a new > story for their lives. Move forward." > > > > -Chuck Palahniuk, "Haunted" > > > > > > On Mon, Sep 9, 2013 at 6:49 PM, joelle weaver > > mailto:joelleweaver@hotmail.com>>wrote: > > > > > All I have is a "histology assistant" description I put together. It > > > is mostly clerical, instrument up keep and other duties. My employer > > > does not hire uncertified techs- due to CLIA license, grossing, FISH > > > & molecular duties ( high complexity). I hope to be a clinical site > > > eventually, and then they will have to complete their certification > > > within one year -if they are hired on. > > > If you think that will help, I can send it on. > > > > > > > > > > > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > > > > > Date: Mon, 9 Sep 2013 15:39:12 -0500 > > > > From: delsuec@gmail.com > > > > To: histonet@lists.utsouthwestern.edu< > mailto:histonet@lists.utsouthwestern.edu> > > > > Subject: [Histonet] Unregistered HT > > > > > > > > Hi all, > > > > > > > > I'm wondering if anyone can share a job description for an > > > > unregistered > > > HT > > > > position. I also need to know if unregistered HT's are allowed to > > > > cut frozen sections. I know there is a limit to what they can do, > > > > especially where IHC and special staining are concerned, but I > > > > really don't know > > > about > > > > frozens, since it's a lot like regular microtomy, except for the > > > > H&E staining of the slide. I also understand that facilities might > > > > have different descriptions, but what I'm really looking for is > > > > what CAP > > > allows > > > > them to do. > > > > > > > > Thanks for your help, > > > > > > > > Deloris Carter, HT(ASCP) > > > > SMMC > > > > Shawnee, KS > > > > _______________________________________________ > > > > Histonet mailing list > > > > Histonet@lists.utsouthwestern.edu< > mailto:Histonet@lists.utsouthwestern.edu> > > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu< > mailto:Histonet@lists.utsouthwestern.edu> > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu< > mailto:Histonet@lists.utsouthwestern.edu> > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ---------------------------------------------------------------------- > > Confidentiality Notice: This e-mail message, including any > > attachments, > is for the sole use of the intended recipient(s) and may contain > confidential and privileged information. Any unauthorized review, use, > disclosure or distribution is prohibited. If you are not the intended > recipient, please contact the sender by reply e-mail and destroy all copies > of the original message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ________________________________ > > This e-mail message (including any attachments) is for the sole use of the > intended recipient(s) and may contain confidential and privileged > information. If the reader of this message is not the intended recipient, > you are hereby notified that any dissemination, distribution or copying of > this message (including any attachments) is strictly prohibited. > > If you have received this message in error, please contact the sender by > reply e-mail message and destroy all copies of the original message > (including attachments). > > ---------------------------------------------------------------------- > Confidentiality Notice: This e-mail message, including any attachments, is > for the sole use of the intended recipient(s) and may contain confidential > and privileged information. Any unauthorized review, use, disclosure or > distribution is prohibited. If you are not the intended recipient, please > contact the sender by reply e-mail and destroy all copies of the original > message. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PAMarcum <@t> uams.edu Wed Sep 11 10:16:03 2013 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Wed Sep 11 10:16:16 2013 Subject: [Histonet] Unregistered HT In-Reply-To: References: <41D3A1AF6FEF0643BDC89E0516A6EA32D31581E1@Mail2Node2.ad.uams.edu> <41D3A1AF6FEF0643BDC89E0516A6EA32D3158371@Mail2Node2.ad.uams.edu> <25A4DE08332B19499904459F00AAACB719C704FD68@EVS1.archildrens.org> <16F90B93CA23D446980B3D591FD02DAD08A835@UWHC-MBX14.uwhis.hosp.wisc.edu> <41D3A1AF6FEF0643BDC89E0516A6EA32D3158578@Mail2Node2.ad.uams.edu> Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA32D31585BD@Mail2Node2.ad.uams.edu> Many states and regions are affiliated with NSH and have state societies that have annual meetings with one or two days of seminars and workshops that are reasonably priced and hopefully close enough to allow attendance. The National Society for Histotechnology has a meeting at a specified location every year that is 5 days long and covers clinical, veterinary, research and other topics in management. The meetings are listed on the NSH.org website if you are interested in looking at all available meetings for most states and regions. These are great educational opportunities for everyone whether you are a registered HT/HTL/student or researcher with special interests. Pam Marcum -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Wednesday, September 11, 2013 9:57 AM To: histonet@lists.utsouthwestern.edu Subject: Fwd: [Histonet] Unregistered HT How do you gain credits? Do you get them for attending seminars or do you have to take actual courses? Are these the CME's I always see noted for lectures? I never knew what those were for. Emily "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." -Chuck Palahniuk, "Haunted" On Wed, Sep 11, 2013 at 10:46 AM, Marcum, Pamela A wrote: > Agreed and now that the registries for HT and HTL require credits to > remain registered it does help with keeping people in the area > learning and growing. The point that other than the front office for NSH is a well > taken point and the rest are volunteers. I am not always sure the front > office understands hospitals, private labs, universities etc. are not > sending anyone to meetings or really encouraging further education for > most lab staffs. Histology here can get registration paid for and > everything else is on the person attending for transportation/room and > board and all other expenses we are not able to go to meetings in far flung places. > > Pam Marcum > > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From ewj <@t> pigsqq.org Wed Sep 11 10:17:39 2013 From: ewj <@t> pigsqq.org (=?UTF-8?B?IkUuIFdheW5lIEpvaG5zb24g5pyx56iz5qOuIg==?=) Date: Wed Sep 11 10:17:51 2013 Subject: [Histonet] (no subject) In-Reply-To: References: Message-ID: <52308993.60808@pigsqq.org> We work in the veterinary medical field and 98% of our work is with swine diseases including some feed additive safety evaluations. We provide pictures of all the lesions that we see as part of the report we provide to farms. It definitely takes more of our time than it would to give a pathomorphologic description in English and Chinese but we believe in transparency even though it might expose us somehow, realizing that some things in pathology are art. We are providing service and we think the best way is to be as transparent as possible in everything that we do. Stonewalling and secrecy generally breed contempt. We try to be at our best even when we dont feel like it. It is true that our subjects have economic value only and they are expendable and the pigs are unlikely to see us in court. But I think that even in situations where there are emotions as with pets, and where there are real human concerns about quality of care for human patients, transparency and participation of all those concerned in the diagnositic and evaluative process generally creates good will and cooperation and good outcomes. On 3:59, CHRISTIE GOWAN wrote: > Hi Clay, > > Most hospitals have a tissue committee that makes decisions about surgical specimens. These decisions become hospital policy. Most hospitals state that once a specimen is removed it essentially becomes the property of the hospital. Release of specimens is usually after it has been accessioned and grossed in for pathology reveiw. There is usually a form for that type of release. The hospital would be at risk if they let the specimen leave without following hospital policy. I am not saying it is right or wrong but I can see the logic in it. Once the specimen has been processed through the hospitals pathology department then blocks and slides can be requested for 2nd opinions. Good luck in your search for answers. > > Christie Gowan > > > > To: histonet@lists.utsouthwestern.edu > Date: Thu, 12 Sep 2013 12:38:10 +0000 > From: claymilks@hotmail.com > Subject: [Histonet] (no subject) > > > > > I need a little help on a Patient?s rights question. > > > It is my understanding that when a patient has a procedure, the patient has the right to request that those specimens be examined by a laboratory of their choosing. i.e. EGD, colonoscopy, etc > > > I am in Arkansas. > > > My father had a procedure yesterday at a local hospital. I manage a pathology laboratory that specializes in the type of tissue that the procedure procured. When it was requested that the tissue be sent to my laboratory, the hospital staff refused to fulfill the request. We asked multiple times for a release form so my father could take his tissue with him. The administration employee we spoke to said there was no such thing and that patients were not allowed to take their specimens. One employee going so far as stating that if we wanted the tissue sent somewhere aside from their contracted laboratory, that the procedure would be canceled and my father would have to go somewhere else. My father, not wanting to cause a fuss, let the issue go. > > > Questions: > > Is it legal for a hospital to require that tissue specimens be sent to a lab they are contracted with? > > > Are there any other actions we could have taken to make our requests be honored? > > > > This is not meant to insult the hospital, but to give an explanation of the situation for context in answering the questions. > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From gu.lang <@t> gmx.at Wed Sep 11 10:19:17 2013 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Wed Sep 11 10:19:24 2013 Subject: [Histonet] edge effect IHC Message-ID: <002101ceaf02$4981ecd0$dc85c670$@gmx.at> Hi! Can someone give me a nice description of the "edge effect" in IHC? Is there a common opinion about the causes? Thank you Gudrun Lang From ttruscot <@t> vetmed.wsu.edu Wed Sep 11 10:21:24 2013 From: ttruscot <@t> vetmed.wsu.edu (Truscott, Tom) Date: Wed Sep 11 10:21:28 2013 Subject: [Histonet] Qdots Message-ID: <9EF5279EBDFE6E4FB6605E8F183A00275BB6E610@CVM76.vetmed.wsu.edu> If anyone has had success with solving background issues with Ventana's Q-Dot labeling, would you please contact me? Thankyou, Tom Truscott From sadrew <@t> wisc.edu Wed Sep 11 10:48:04 2013 From: sadrew <@t> wisc.edu (Sally Ann Drew) Date: Wed Sep 11 10:48:07 2013 Subject: [Histonet] Qdots In-Reply-To: <9EF5279EBDFE6E4FB6605E8F183A00275BB6E610@CVM76.vetmed.wsu.edu> References: <9EF5279EBDFE6E4FB6605E8F183A00275BB6E610@CVM76.vetmed.wsu.edu> Message-ID: <009f01ceaf06$4d926f30$e8b74d90$@wisc.edu> Please share the info? We're just beginning to use Qdots-but haven't yet on the Discovery, so it would be nice to have a heads-up about any issues others are having! Thank you! _Sally Sally Ann Drew,MT(ASCP) Dept. of Pathology TRIP Lab Manager CSC, K4/435 608.265.4378 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Truscott, Tom Sent: Wednesday, September 11, 2013 10:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Qdots If anyone has had success with solving background issues with Ventana's Q-Dot labeling, would you please contact me? Thankyou, Tom Truscott _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Wed Sep 11 10:54:03 2013 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Wed Sep 11 10:54:19 2013 Subject: [Histonet] Unregistered HT In-Reply-To: <25A4DE08332B19499904459F00AAACB719C704FD68@EVS1.archildrens.org> References: , , , <41D3A1AF6FEF0643BDC89E0516A6EA32D31581E1@Mail2Node2.ad.uams.edu> <41D3A1AF6FEF0643BDC89E0516A6EA32D3158371@Mail2Node2.ad.uams.edu> <25A4DE08332B19499904459F00AAACB719C704FD68@EVS1.archildrens.org> Message-ID: <761E2B5697F795489C8710BCC72141FF0992DB@ex07.net.ucsf.edu> This discussion comes up every year and, of course is never resolved to our satisfaction because histotechs really have only a little say in it. Personally I think labs are better off with certified techs. Uncertified techs should start as lab assistants and earn their certification before getting a histotech title. The histotech title should be for someone who at least starts as a general histotech who can do the gamut of histology work - embedding, cutting, H&E, specials and IHC/ISH. From that base they can branch out or specialize. It would be a dream to require formal schooling, as with med techs, but there are so few schools that it is just impractical. A certification is a baseline that shows a person who is OJT has learned the minimum required to understand histotechnology. However, because we don't report out any results on our own authority there is no impetus for a pathologist or institution to make the position more than it is: a technical position that does do require fancy work, but in the end "only" provides the materials for someone else to use for interpretation, decisions and reporting. We don't even pick the sample, the test or even determine how to do the test - that is all determined by the Technical Director of the lab, usually a board certified pathologist. Our primary job is to provide good quality materials so they can do their job well. That does involved a lot of knowledge, organization and skill, but it is not at the level of making actual patient care decisions. Pathologists Assistants get a bit more "respect" because they are required to make judgment decisions on how to sample a particular case. Even then, that sampling is strictly detailed in procedures developed by pathologists. The most we could ask for is licensing that requires certain qualifications to be a histotech. However, that has some drawbacks as well, namely the restriction of the profession to licensed people, and so is a barrier to entry. Would it lead to pay raises? Does anyone have studies showing pay before and after licesure requirements? It is questionable whether it enhances the quality of the lab since most histotechs are OJT anyway, and simple licensure may not increase actual quality of work by an individual. (does anyone know of any studies that look at quality in states with and without licensing?). The CLIA requirements at least set a baseline for education, if not actual certification. Asking pathologists to support universal certification and/or licensure is problematic - many independent labs won't support that because, as in licensure, it decreases the pool and increases costs (ie, pay). Since the pathologist is the person deemed responsible for quality and lab results, setting the bar higher is only in the interests of the technologists, not the pathologist. Now, some enlightened pathologists understand that better-educated and better-trained techs are good for the overall. So in the end the histotech community, along with a few enlightened pathologists have to lobby for anything they want. But what is that? Increased pay? More say in lab operations? My experience is that you can rise as high as you want if you take opportunities that come up. But that may mean either spending many years in one place slowly moving up, or moving around to take other opportunities. It will depend on the individual. In either case using your time in the lab to learn whatever there is to learn, even in other departments, or on various committees, pays off in the long run. And that includes management. The last thing you should do is limit yourself to a job description someone gives you. Take that as the base line, not the limit. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Wednesday, September 11, 2013 6:30 AM To: 'Weems, Joyce K.'; 'Jennifer MacDonald'; Marcum, Pamela A Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT Histology does not get the respect or the recognition because histologists do not report results. All of the complex testing we do is overlooked because the pathologists report the results. CLIA standards are based on result reporting. The CAP has looked the other way for years because pathologists would hire unregistered techs. If pathologists would demand only registered techs half our battle would be won. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Wednesday, September 11, 2013 8:08 AM To: 'Jennifer MacDonald'; Marcum, Pamela A Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT And the reason so many have been fighting for this for years. If a lab were looking for a Medical Technologist there would be no question. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Tuesday, September 10, 2013 7:32 PM To: Marcum, Pamela A Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT As long as we do not need certification, licensure and minium education requirements we will not be recognized as Laboratory Professionals. From: "Marcum, Pamela A" To: "'joelle weaver'" , "'Emily Sours'" , "histonet@lists.utsouthwestern.edu" Date: 09/10/2013 01:12 PM Subject: RE: [Histonet] Unregistered HT Sent by: histonet-bounces@lists.utsouthwestern.edu I agree we have huge gray areas and not all histology schools are as good as they could be for what we are facing in Histology. I keep harping on the fact that until we are recognized as Laboratory Professionals we will stay in this limbo. The rules determining complex testing should be revisited to what is done in Histology Laboratories today and not what we did 30 or more years ago. The Clinical Laboratory is now so automated it is hard to find anyone in most areas who can even remember doing any manual testing. The Micro lab is the closest to being as manual as areas of Histology. I am in a small market and finding a registered Histologist is harder for us. I would love to have 8 to choose from and interview. Pam Marcum From: joelle weaver [mailto:joelleweaver@hotmail.com] Sent: Tuesday, September 10, 2013 2:59 PM To: Marcum, Pamela A; 'Emily Sours'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT Well I am mostly clinical...but I think that organizations can set standards outside and beyond what CAP,CLIA etc stipulate. For the position I have now, I had to submit all my transcripts from high school up through masters in addition to proof of my ASCP certification, IHC qualification, continuing education, and professional association activity. There is a lot of gray area out there. They seem to have not had trouble getting applicants though ( and I know this varies by market), there were over 8 candidates for an HT opening, which I thought was a pretty good turn out. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: PAMarcum@uams.edu > To: talulahgosh@gmail.com; joelleweaver@hotmail.com > CC: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Unregistered HT > Date: Tue, 10 Sep 2013 13:53:26 +0000 > > Research is a different area and not controlled by CAP, CLIA and other hospital licensing or accreditation organizations. We are bound by the rules of these organizations and while I agree with you to a point. We do need minimums for training and registration by recognized licensing bodies when patient tissue is being processed for histological examination. I am sure no one thinks of this often however; there are medical legal issues with insurance we have that do not apply for research. It is also clear that registration does not mean we don't have registered people who are not as good as they should be for excellent patient care. > > I have worked in research and while I would not ever say the hiring of non-registered people is a problem for research. It is often a specialty area that requires knowing more than routine Histology. I have done plastics in research that could not ever be used in routine Histology due to the time factors and in some cases limited use with staining applications, especially IHC for some procedures. Many other areas in research require more specialized training than would be used in a routine area. I would also add some really great techs are in many phases of research. I know MT who work in Histology and are not registered as the MT BS, overrides the HT requirement for many institutions. > > Many factors must be considered for both research and routine > Histology that cover far more than just hiring registered people in certain areas of the laboratory. > > Pam Marcum > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu< mailto:histonet-bounces@lists.utsouthwestern.edu> [ mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours > Sent: Tuesday, September 10, 2013 8:20 AM > To: joelle weaver > Cc: histonet@lists.utsouthwestern.edu< mailto:histonet@lists.utsouthwestern.edu> > Subject: Re: [Histonet] Unregistered HT > > Do employers consider lab techs to be proficient enough? I've been > doing ISH and immuno for 13 years, but I'm not certified as I do research. Maybe there aren't a lot of lab techs out there? Just wondering since you might be missing someone awesome by hiring only certified people. > > "By bitching and bitching and bitching, they could exhaust the drama > of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." > > -Chuck Palahniuk, "Haunted" > > > On Mon, Sep 9, 2013 at 6:49 PM, joelle weaver > >wrote: > > > All I have is a "histology assistant" description I put together. It > > is mostly clerical, instrument up keep and other duties. My employer > > does not hire uncertified techs- due to CLIA license, grossing, FISH > > & molecular duties ( high complexity). I hope to be a clinical site > > eventually, and then they will have to complete their certification > > within one year -if they are hired on. > > If you think that will help, I can send it on. > > > > > > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > > > Date: Mon, 9 Sep 2013 15:39:12 -0500 > > > From: delsuec@gmail.com > > > To: histonet@lists.utsouthwestern.edu< mailto:histonet@lists.utsouthwestern.edu> > > > Subject: [Histonet] Unregistered HT > > > > > > Hi all, > > > > > > I'm wondering if anyone can share a job description for an > > > unregistered > > HT > > > position. I also need to know if unregistered HT's are allowed to > > > cut frozen sections. I know there is a limit to what they can do, > > > especially where IHC and special staining are concerned, but I > > > really don't know > > about > > > frozens, since it's a lot like regular microtomy, except for the > > > H&E staining of the slide. I also understand that facilities might > > > have different descriptions, but what I'm really looking for is > > > what CAP > > allows > > > them to do. > > > > > > Thanks for your help, > > > > > > Deloris Carter, HT(ASCP) > > > SMMC > > > Shawnee, KS > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu< mailto:Histonet@lists.utsouthwestern.edu> > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu< mailto:Histonet@lists.utsouthwestern.edu> > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu< mailto:Histonet@lists.utsouthwestern.edu> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ---------------------------------------------------------------------- > Confidentiality Notice: This e-mail message, including any > attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HornHV <@t> archildrens.org Wed Sep 11 10:59:11 2013 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Wed Sep 11 10:59:18 2013 Subject: [Histonet] Unregistered HT In-Reply-To: <761E2B5697F795489C8710BCC72141FF0992DB@ex07.net.ucsf.edu> References: , , , <41D3A1AF6FEF0643BDC89E0516A6EA32D31581E1@Mail2Node2.ad.uams.edu> <41D3A1AF6FEF0643BDC89E0516A6EA32D3158371@Mail2Node2.ad.uams.edu> <25A4DE08332B19499904459F00AAACB719C704FD68@EVS1.archildrens.org> <761E2B5697F795489C8710BCC72141FF0992DB@ex07.net.ucsf.edu> Message-ID: <25A4DE08332B19499904459F00AAACB719C704FD6A@EVS1.archildrens.org> Well said, Tim. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Wednesday, September 11, 2013 10:54 AM To: Histonet Subject: RE: [Histonet] Unregistered HT This discussion comes up every year and, of course is never resolved to our satisfaction because histotechs really have only a little say in it. Personally I think labs are better off with certified techs. Uncertified techs should start as lab assistants and earn their certification before getting a histotech title. The histotech title should be for someone who at least starts as a general histotech who can do the gamut of histology work - embedding, cutting, H&E, specials and IHC/ISH. From that base they can branch out or specialize. It would be a dream to require formal schooling, as with med techs, but there are so few schools that it is just impractical. A certification is a baseline that shows a person who is OJT has learned the minimum required to understand histotechnology. However, because we don't report out any results on our own authority there is no impetus for a pathologist or institution to make the position more than it is: a technical position that does do require fancy work, but in the end "only" provides the materials for someone else to use for interpretation, decisions and reporting. We don't even pick the sample, the test or even determine how to do the test - that is all determined by the Technical Director of the lab, usually a board certified pathologist. Our primary job is to provide good quality materials so they can do their job well. That does involved a lot of knowledge, organization and skill, but it is not at the level of making actual patient care decisions. Pathologists Assistants get a bit more "respect" because they are required to make judgment decisions on how to sample a particular case. Even then, that sampling is strictly detailed in procedures developed by pathologists. The most we could ask for is licensing that requires certain qualifications to be a histotech. However, that has some drawbacks as well, namely the restriction of the profession to licensed people, and so is a barrier to entry. Would it lead to pay raises? Does anyone have studies showing pay before and after licesure requirements? It is questionable whether it enhances the quality of the lab since most histotechs are OJT anyway, and simple licensure may not increase actual quality of work by an individual. (does anyone know of any studies that look at quality in states with and without licensing?). The CLIA requirements at least set a baseline for education, if not actual certification. Asking pathologists to support universal certification and/or licensure is problematic - many independent labs won't support that because, as in licensure, it decreases the pool and increases costs (ie, pay). Since the pathologist is the person deemed responsible for quality and lab results, setting the bar higher is only in the interests of the technologists, not the pathologist. Now, some enlightened pathologists understand that better-educated and better-trained techs are good for the overall. So in the end the histotech community, along with a few enlightened pathologists have to lobby for anything they want. But what is that? Increased pay? More say in lab operations? My experience is that you can rise as high as you want if you take opportunities that come up. But that may mean either spending many years in one place slowly moving up, or moving around to take other opportunities. It will depend on the individual. In either case using your time in the lab to learn whatever there is to learn, even in other departments, or on various committees, pays off in the long run. And that includes management. The last thing you should do is limit yourself to a job description someone gives you. Take that as the base line, not the limit. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Wednesday, September 11, 2013 6:30 AM To: 'Weems, Joyce K.'; 'Jennifer MacDonald'; Marcum, Pamela A Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT Histology does not get the respect or the recognition because histologists do not report results. All of the complex testing we do is overlooked because the pathologists report the results. CLIA standards are based on result reporting. The CAP has looked the other way for years because pathologists would hire unregistered techs. If pathologists would demand only registered techs half our battle would be won. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Wednesday, September 11, 2013 8:08 AM To: 'Jennifer MacDonald'; Marcum, Pamela A Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT And the reason so many have been fighting for this for years. If a lab were looking for a Medical Technologist there would be no question. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Tuesday, September 10, 2013 7:32 PM To: Marcum, Pamela A Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT As long as we do not need certification, licensure and minium education requirements we will not be recognized as Laboratory Professionals. From: "Marcum, Pamela A" To: "'joelle weaver'" , "'Emily Sours'" , "histonet@lists.utsouthwestern.edu" Date: 09/10/2013 01:12 PM Subject: RE: [Histonet] Unregistered HT Sent by: histonet-bounces@lists.utsouthwestern.edu I agree we have huge gray areas and not all histology schools are as good as they could be for what we are facing in Histology. I keep harping on the fact that until we are recognized as Laboratory Professionals we will stay in this limbo. The rules determining complex testing should be revisited to what is done in Histology Laboratories today and not what we did 30 or more years ago. The Clinical Laboratory is now so automated it is hard to find anyone in most areas who can even remember doing any manual testing. The Micro lab is the closest to being as manual as areas of Histology. I am in a small market and finding a registered Histologist is harder for us. I would love to have 8 to choose from and interview. Pam Marcum From: joelle weaver [mailto:joelleweaver@hotmail.com] Sent: Tuesday, September 10, 2013 2:59 PM To: Marcum, Pamela A; 'Emily Sours'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT Well I am mostly clinical...but I think that organizations can set standards outside and beyond what CAP,CLIA etc stipulate. For the position I have now, I had to submit all my transcripts from high school up through masters in addition to proof of my ASCP certification, IHC qualification, continuing education, and professional association activity. There is a lot of gray area out there. They seem to have not had trouble getting applicants though ( and I know this varies by market), there were over 8 candidates for an HT opening, which I thought was a pretty good turn out. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: PAMarcum@uams.edu > To: talulahgosh@gmail.com; joelleweaver@hotmail.com > CC: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Unregistered HT > Date: Tue, 10 Sep 2013 13:53:26 +0000 > > Research is a different area and not controlled by CAP, CLIA and other hospital licensing or accreditation organizations. We are bound by the rules of these organizations and while I agree with you to a point. We do need minimums for training and registration by recognized licensing bodies when patient tissue is being processed for histological examination. I am sure no one thinks of this often however; there are medical legal issues with insurance we have that do not apply for research. It is also clear that registration does not mean we don't have registered people who are not as good as they should be for excellent patient care. > > I have worked in research and while I would not ever say the hiring of non-registered people is a problem for research. It is often a specialty area that requires knowing more than routine Histology. I have done plastics in research that could not ever be used in routine Histology due to the time factors and in some cases limited use with staining applications, especially IHC for some procedures. Many other areas in research require more specialized training than would be used in a routine area. I would also add some really great techs are in many phases of research. I know MT who work in Histology and are not registered as the MT BS, overrides the HT requirement for many institutions. > > Many factors must be considered for both research and routine > Histology that cover far more than just hiring registered people in certain areas of the laboratory. > > Pam Marcum > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu< mailto:histonet-bounces@lists.utsouthwestern.edu> [ mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours > Sent: Tuesday, September 10, 2013 8:20 AM > To: joelle weaver > Cc: histonet@lists.utsouthwestern.edu< mailto:histonet@lists.utsouthwestern.edu> > Subject: Re: [Histonet] Unregistered HT > > Do employers consider lab techs to be proficient enough? I've been > doing ISH and immuno for 13 years, but I'm not certified as I do research. Maybe there aren't a lot of lab techs out there? Just wondering since you might be missing someone awesome by hiring only certified people. > > "By bitching and bitching and bitching, they could exhaust the drama > of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." > > -Chuck Palahniuk, "Haunted" > > > On Mon, Sep 9, 2013 at 6:49 PM, joelle weaver > >wrote: > > > All I have is a "histology assistant" description I put together. It > > is mostly clerical, instrument up keep and other duties. My employer > > does not hire uncertified techs- due to CLIA license, grossing, FISH > > & molecular duties ( high complexity). I hope to be a clinical site > > eventually, and then they will have to complete their certification > > within one year -if they are hired on. > > If you think that will help, I can send it on. > > > > > > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > > > Date: Mon, 9 Sep 2013 15:39:12 -0500 > > > From: delsuec@gmail.com > > > To: histonet@lists.utsouthwestern.edu< mailto:histonet@lists.utsouthwestern.edu> > > > Subject: [Histonet] Unregistered HT > > > > > > Hi all, > > > > > > I'm wondering if anyone can share a job description for an > > > unregistered > > HT > > > position. I also need to know if unregistered HT's are allowed to > > > cut frozen sections. I know there is a limit to what they can do, > > > especially where IHC and special staining are concerned, but I > > > really don't know > > about > > > frozens, since it's a lot like regular microtomy, except for the > > > H&E staining of the slide. I also understand that facilities might > > > have different descriptions, but what I'm really looking for is > > > what CAP > > allows > > > them to do. > > > > > > Thanks for your help, > > > > > > Deloris Carter, HT(ASCP) > > > SMMC > > > Shawnee, KS > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu< mailto:Histonet@lists.utsouthwestern.edu> > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu< mailto:Histonet@lists.utsouthwestern.edu> > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu< mailto:Histonet@lists.utsouthwestern.edu> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ---------------------------------------------------------------------- > Confidentiality Notice: This e-mail message, including any > attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From billodonnell <@t> catholichealth.net Wed Sep 11 11:02:21 2013 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Wed Sep 11 11:02:35 2013 Subject: [Histonet] Unregistered HT In-Reply-To: <484323625.958457.1378907135764.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> References: <484323625.958457.1378907135764.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Message-ID: This is precisely why I stopped giving NSH my money. When they start to earn it, I will reinstate my membership. I suppose they could argue that they can't do it without my money - they had 25 years of my membership - and all I got was a discount to meetings my institution can't afford to send me to and, I will admit, a decent magazine.. Now if I could just withhold taxes until the Government starts doing what we elected it to do... WAAAAAy off topic- Bill -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pam Marcum Sent: Wednesday, September 11, 2013 8:46 AM To: Joyce K. Weems Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Unregistered HT We also need NSH to step up and do what they said they would years ago instead of planning more and more meetings per year and increasing what we pay them for not doing anything to help us.? They seem to support anything ASCP/CAP/CLIA etc tells them and we are left footing the bill in more ways than monetarily.? NSH in Providence is in a week and I cannot go this time so why can't those going start asking where the proposal brought up years ago hasn't moved forward.? I would have been happy to bring it up from the floor in the general membership meeting.? Pam Marcum ----- Original Message ----- From: "Joyce K. Weems" To: histonet@lists.utsouthwestern.edu Sent: Wednesday, September 11, 2013 8:39:03 AM Subject: RE: [Histonet] Unregistered HT They are only as good as we are, so we need them to fight for us!! Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). ?It may contain information that is privileged and confidential. ?Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: Horn, Hazel V [mailto:HornHV@archildrens.org] Sent: Wednesday, September 11, 2013 9:30 AM To: Weems, Joyce K.; 'Jennifer MacDonald'; Marcum, Pamela A Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT Histology does not get the respect or the recognition because histologists do not report results. ?All of the complex testing we do is overlooked because the pathologists report the results. ? CLIA standards are based on result reporting. ? The CAP has looked the other way for years because pathologists would hire unregistered techs. ?If pathologists would demand only registered techs half our battle would be won. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Wednesday, September 11, 2013 8:08 AM To: 'Jennifer MacDonald'; Marcum, Pamela A Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT And the reason so many have been fighting for this for years. If a lab were looking for a Medical Technologist there would be no question. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). ?It may contain information that is privileged and confidential. ?Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Tuesday, September 10, 2013 7:32 PM To: Marcum, Pamela A Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT As long as we do not need certification, licensure and minium education requirements we will not be recognized as Laboratory Professionals. From: ? "Marcum, Pamela A" To: ? ? "'joelle weaver'" , "'Emily Sours'" , "histonet@lists.utsouthwestern.edu" Date: ? 09/10/2013 01:12 PM Subject: ? ? ? ?RE: [Histonet] Unregistered HT Sent by: ? ? ? ?histonet-bounces@lists.utsouthwestern.edu I agree we have huge gray areas and not all histology schools are as good as they could be for what we are facing in Histology. ?I keep harping on the fact that until we are recognized as Laboratory Professionals we will stay in this limbo. ?The rules determining complex testing should be revisited to what is done in Histology Laboratories today and not what we did 30 or more years ago. ?The Clinical Laboratory is now so automated it is hard to find anyone in most areas who can even remember doing any manual testing. ?The Micro lab is the closest to being as manual as areas of Histology. I am in a small market and finding a registered Histologist is harder for us. ?I would love to have 8 to choose from and interview. Pam Marcum From: joelle weaver [mailto:joelleweaver@hotmail.com] Sent: Tuesday, September 10, 2013 2:59 PM To: Marcum, Pamela A; 'Emily Sours'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT ?Well I am ?mostly clinical...but I think that organizations can set standards outside and beyond what CAP,CLIA etc stipulate. For the position I have now, I had to submit all my transcripts from high school up through masters in addition to ?proof of my ASCP certification, IHC qualification, continuing education, and professional association activity. There is a lot of gray area out there. They seem to have not had trouble getting applicants though ( and I know this varies by market), there were over 8 candidates for an HT opening, which I thought was a pretty good turn out. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: PAMarcum@uams.edu > To: talulahgosh@gmail.com; joelleweaver@hotmail.com > CC: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Unregistered HT > Date: Tue, 10 Sep 2013 13:53:26 +0000 > > Research is a different area and not controlled by CAP, CLIA and other hospital licensing or accreditation organizations. We are bound by the rules of these organizations and while I agree with you to a point. We do need minimums for training and registration by recognized licensing bodies when patient tissue is being processed for histological examination. I am sure no one thinks of this often however; there are medical legal issues with insurance we have that do not apply for research. It is also clear that registration does not mean we don't have registered people who are not as good as they should be for excellent patient care. > > I have worked in research and while I would not ever say the hiring of non-registered people is a problem for research. It is often a specialty area that requires knowing more than routine Histology. I have done plastics in research that could not ever be used in routine Histology due to the time factors and in some cases limited use with staining applications, especially IHC for some procedures. Many other areas in research require more specialized training than would be used in a routine area. I would also add some really great techs are in many phases of research. I know MT who work in Histology and are not registered as the MT BS, overrides the HT requirement for many institutions. > > Many factors must be considered for both research and routine > Histology that cover far more than just hiring registered people in certain areas of the laboratory. > > Pam Marcum > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu< mailto:histonet-bounces@lists.utsouthwestern.edu> [ mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours > Sent: Tuesday, September 10, 2013 8:20 AM > To: joelle weaver > Cc: histonet@lists.utsouthwestern.edu< mailto:histonet@lists.utsouthwestern.edu> > Subject: Re: [Histonet] Unregistered HT > > Do employers consider lab techs to be proficient enough? I've been > doing ISH and immuno for 13 years, but I'm not certified as I do research. Maybe there aren't a lot of lab techs out there? Just wondering since you might be missing someone awesome by hiring only certified people. > > "By bitching and bitching and bitching, they could exhaust the drama > of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." > > -Chuck Palahniuk, "Haunted" > > > On Mon, Sep 9, 2013 at 6:49 PM, joelle weaver > >wrote: > > > All I have is a "histology assistant" description I put together. It > > is mostly clerical, instrument up keep and other duties. My employer > > does not hire uncertified techs- due to CLIA license, grossing, FISH > > & molecular duties ( high complexity). I hope to be a clinical site > > eventually, and then they will have to complete their certification > > within one year -if they are hired on. > > If you think that will help, I can send it on. > > > > > > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > > > Date: Mon, 9 Sep 2013 15:39:12 -0500 > > > From: delsuec@gmail.com > > > To: histonet@lists.utsouthwestern.edu< mailto:histonet@lists.utsouthwestern.edu> > > > Subject: [Histonet] Unregistered HT > > > > > > Hi all, > > > > > > I'm wondering if anyone can share a job description for an > > > unregistered > > HT > > > position. I also need to know if unregistered HT's are allowed to > > > cut frozen sections. I know there is a limit to what they can do, > > > especially where IHC and special staining are concerned, but I > > > really don't know > > about > > > frozens, since it's a lot like regular microtomy, except for the > > > H&E staining of the slide. I also understand that facilities might > > > have different descriptions, but what I'm really looking for is > > > what CAP > > allows > > > them to do. > > > > > > Thanks for your help, > > > > > > Deloris Carter, HT(ASCP) > > > SMMC > > > Shawnee, KS > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu< mailto:Histonet@lists.utsouthwestern.edu> > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu< mailto:Histonet@lists.utsouthwestern.edu> > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu< mailto:Histonet@lists.utsouthwestern.edu> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ---------------------------------------------------------------------- > Confidentiality Notice: This e-mail message, including any > attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. From Richard.Cartun <@t> hhchealth.org Wed Sep 11 11:13:48 2013 From: Richard.Cartun <@t> hhchealth.org (Cartun, Richard) Date: Wed Sep 11 11:13:55 2013 Subject: [Histonet] (no subject) In-Reply-To: References: Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E01882970@HHCEXCHMB05.hhcsystem.org> Hospital "By-Laws" established by the Medical Staff and Administration may prevent tissue obtained within the hospital from going outside to another laboratory. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax richard.cartun@hhchealth.org ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of clay milks [claymilks@hotmail.com] Sent: Thursday, September 12, 2013 8:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) I need a little help on a Patient?s rights question. It is my understanding that when a patient has a procedure, the patient has the right to request that those specimens be examined by a laboratory of their choosing. i.e. EGD, colonoscopy, etc I am in Arkansas. My father had a procedure yesterday at a local hospital. I manage a pathology laboratory that specializes in the type of tissue that the procedure procured. When it was requested that the tissue be sent to my laboratory, the hospital staff refused to fulfill the request. We asked multiple times for a release form so my father could take his tissue with him. The administration employee we spoke to said there was no such thing and that patients were not allowed to take their specimens. One employee going so far as stating that if we wanted the tissue sent somewhere aside from their contracted laboratory, that the procedure would be canceled and my father would have to go somewhere else. My father, not wanting to cause a fuss, let the issue go. Questions: Is it legal for a hospital to require that tissue specimens be sent to a lab they are contracted with? Are there any other actions we could have taken to make our requests be honored? This is not meant to insult the hospital, but to give an explanation of the situation for context in answering the questions. This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From Timothy.Morken <@t> ucsfmedctr.org Wed Sep 11 11:35:53 2013 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Wed Sep 11 11:36:07 2013 Subject: [Histonet] edge effect IHC In-Reply-To: <002101ceaf02$4981ecd0$dc85c670$@gmx.at> References: <002101ceaf02$4981ecd0$dc85c670$@gmx.at> Message-ID: <761E2B5697F795489C8710BCC72141FF09934D@ex07.net.ucsf.edu> I've seen edge effect in two instances: 1) Fixation artifact: the tissue is differentially fixed so the edge is fixed more than the center. That makes staining different on the edge vs the center. This occurs when a piece is fixed whole, not sliced. Or could be a thick piece of tissue that does not get fully fixed in the center. The staining will be best in the area that has the proper fixation - and that could be the edge or the interior, or there may be a gradient of staining toward the interior and the "proper" staining will be seen somewhere on the gradient. 2) Drying artifact: liquid applied to the slide dries around the edges of the tissue so the outside edge is dry, or almost dry, and the center is still wet. Usually in this case the outside edge will have a lot of background, the interior will often be properly stained. The edge could be differentially moist or dry, so the staining or background is inconsistent in the tissue. Tim Morken Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: Wednesday, September 11, 2013 8:19 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] edge effect IHC Hi! Can someone give me a nice description of the "edge effect" in IHC? Is there a common opinion about the causes? Thank you Gudrun Lang _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JEllin <@t> yumaregional.org Wed Sep 11 11:41:50 2013 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Wed Sep 11 11:42:04 2013 Subject: [Histonet] Unregistered HT In-Reply-To: <761E2B5697F795489C8710BCC72141FF0992DB@ex07.net.ucsf.edu> References: , , , <41D3A1AF6FEF0643BDC89E0516A6EA32D31581E1@Mail2Node2.ad.uams.edu> <41D3A1AF6FEF0643BDC89E0516A6EA32D3158371@Mail2Node2.ad.uams.edu> <25A4DE08332B19499904459F00AAACB719C704FD68@EVS1.archildrens.org> <761E2B5697F795489C8710BCC72141FF0992DB@ex07.net.ucsf.edu> Message-ID: Tim Well said!!!!!!!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Wednesday, September 11, 2013 8:54 AM To: Histonet Subject: RE: [Histonet] Unregistered HT This discussion comes up every year and, of course is never resolved to our satisfaction because histotechs really have only a little say in it. Personally I think labs are better off with certified techs. Uncertified techs should start as lab assistants and earn their certification before getting a histotech title. The histotech title should be for someone who at least starts as a general histotech who can do the gamut of histology work - embedding, cutting, H&E, specials and IHC/ISH. From that base they can branch out or specialize. It would be a dream to require formal schooling, as with med techs, but there are so few schools that it is just impractical. A certification is a baseline that shows a person who is OJT has learned the minimum required to understand histotechnology. However, because we don't report out any results on our own authority there is no impetus for a pathologist or institution to make the position more than it is: a technical position that does do require fancy work, but in the end "only" provides the materials for someone else to use for interpretation, decisions and reporting. We don't even pick the sample, the test or even determine how to do the test - that is all determined by the Technical Director of the lab, usually a board certified pathologist. Our primary job is to provide good quality materials so they can do their job well. That does involved a lot of knowledge, organization and skill, but it is not at the level of making actual patient care decisions. Pathologists Assistants get a bit more "respect" because they are required to make judgment decisions on how to sample a particular case. Even then, that sampling is strictly detailed in procedures developed by pathologists. The most we could ask for is licensing that requires certain qualifications to be a histotech. However, that has some drawbacks as well, namely the restriction of the profession to licensed people, and so is a barrier to entry. Would it lead to pay raises? Does anyone have studies showing pay before and after licesure requirements? It is questionable whether it enhances the quality of the lab since most histotechs are OJT anyway, and simple licensure may not increase actual quality of work by an individual. (does anyone know of any studies that look at quality in states with and without licensing?). The CLIA requirements at least set a baseline for education, if not actual certification. Asking pathologists to support universal certification and/or licensure is problematic - many independent labs won't support that because, as in licensure, it decreases the pool and increases costs (ie, pay). Since the pathologist is the person deemed responsible for quality and lab results, setting the bar higher is only in the interests of the technologists, not the pathologist. Now, some enlightened pathologists understand that better-educated and better-trained techs are good for the overall. So in the end the histotech community, along with a few enlightened pathologists have to lobby for anything they want. But what is that? Increased pay? More say in lab operations? My experience is that you can rise as high as you want if you take opportunities that come up. But that may mean either spending many years in one place slowly moving up, or moving around to take other opportunities. It will depend on the individual. In either case using your time in the lab to learn whatever there is to learn, even in other departments, or on various committees, pays off in the long run. And that includes management. The last thing you should do is limit yourself to a job description someone gives you. Take that as the base line, not the limit. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Wednesday, September 11, 2013 6:30 AM To: 'Weems, Joyce K.'; 'Jennifer MacDonald'; Marcum, Pamela A Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT Histology does not get the respect or the recognition because histologists do not report results. All of the complex testing we do is overlooked because the pathologists report the results. CLIA standards are based on result reporting. The CAP has looked the other way for years because pathologists would hire unregistered techs. If pathologists would demand only registered techs half our battle would be won. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Wednesday, September 11, 2013 8:08 AM To: 'Jennifer MacDonald'; Marcum, Pamela A Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT And the reason so many have been fighting for this for years. If a lab were looking for a Medical Technologist there would be no question. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Tuesday, September 10, 2013 7:32 PM To: Marcum, Pamela A Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT As long as we do not need certification, licensure and minium education requirements we will not be recognized as Laboratory Professionals. From: "Marcum, Pamela A" To: "'joelle weaver'" , "'Emily Sours'" , "histonet@lists.utsouthwestern.edu" Date: 09/10/2013 01:12 PM Subject: RE: [Histonet] Unregistered HT Sent by: histonet-bounces@lists.utsouthwestern.edu I agree we have huge gray areas and not all histology schools are as good as they could be for what we are facing in Histology. I keep harping on the fact that until we are recognized as Laboratory Professionals we will stay in this limbo. The rules determining complex testing should be revisited to what is done in Histology Laboratories today and not what we did 30 or more years ago. The Clinical Laboratory is now so automated it is hard to find anyone in most areas who can even remember doing any manual testing. The Micro lab is the closest to being as manual as areas of Histology. I am in a small market and finding a registered Histologist is harder for us. I would love to have 8 to choose from and interview. Pam Marcum From: joelle weaver [mailto:joelleweaver@hotmail.com] Sent: Tuesday, September 10, 2013 2:59 PM To: Marcum, Pamela A; 'Emily Sours'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT Well I am mostly clinical...but I think that organizations can set standards outside and beyond what CAP,CLIA etc stipulate. For the position I have now, I had to submit all my transcripts from high school up through masters in addition to proof of my ASCP certification, IHC qualification, continuing education, and professional association activity. There is a lot of gray area out there. They seem to have not had trouble getting applicants though ( and I know this varies by market), there were over 8 candidates for an HT opening, which I thought was a pretty good turn out. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: PAMarcum@uams.edu > To: talulahgosh@gmail.com; joelleweaver@hotmail.com > CC: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Unregistered HT > Date: Tue, 10 Sep 2013 13:53:26 +0000 > > Research is a different area and not controlled by CAP, CLIA and other hospital licensing or accreditation organizations. We are bound by the rules of these organizations and while I agree with you to a point. We do need minimums for training and registration by recognized licensing bodies when patient tissue is being processed for histological examination. I am sure no one thinks of this often however; there are medical legal issues with insurance we have that do not apply for research. It is also clear that registration does not mean we don't have registered people who are not as good as they should be for excellent patient care. > > I have worked in research and while I would not ever say the hiring of non-registered people is a problem for research. It is often a specialty area that requires knowing more than routine Histology. I have done plastics in research that could not ever be used in routine Histology due to the time factors and in some cases limited use with staining applications, especially IHC for some procedures. Many other areas in research require more specialized training than would be used in a routine area. I would also add some really great techs are in many phases of research. I know MT who work in Histology and are not registered as the MT BS, overrides the HT requirement for many institutions. > > Many factors must be considered for both research and routine > Histology that cover far more than just hiring registered people in certain areas of the laboratory. > > Pam Marcum > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu< mailto:histonet-bounces@lists.utsouthwestern.edu> [ mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours > Sent: Tuesday, September 10, 2013 8:20 AM > To: joelle weaver > Cc: histonet@lists.utsouthwestern.edu< mailto:histonet@lists.utsouthwestern.edu> > Subject: Re: [Histonet] Unregistered HT > > Do employers consider lab techs to be proficient enough? I've been > doing ISH and immuno for 13 years, but I'm not certified as I do research. Maybe there aren't a lot of lab techs out there? Just wondering since you might be missing someone awesome by hiring only certified people. > > "By bitching and bitching and bitching, they could exhaust the drama > of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." > > -Chuck Palahniuk, "Haunted" > > > On Mon, Sep 9, 2013 at 6:49 PM, joelle weaver > >wrote: > > > All I have is a "histology assistant" description I put together. It > > is mostly clerical, instrument up keep and other duties. My employer > > does not hire uncertified techs- due to CLIA license, grossing, FISH > > & molecular duties ( high complexity). I hope to be a clinical site > > eventually, and then they will have to complete their certification > > within one year -if they are hired on. > > If you think that will help, I can send it on. > > > > > > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > > > Date: Mon, 9 Sep 2013 15:39:12 -0500 > > > From: delsuec@gmail.com > > > To: histonet@lists.utsouthwestern.edu< mailto:histonet@lists.utsouthwestern.edu> > > > Subject: [Histonet] Unregistered HT > > > > > > Hi all, > > > > > > I'm wondering if anyone can share a job description for an > > > unregistered > > HT > > > position. I also need to know if unregistered HT's are allowed to > > > cut frozen sections. I know there is a limit to what they can do, > > > especially where IHC and special staining are concerned, but I > > > really don't know > > about > > > frozens, since it's a lot like regular microtomy, except for the > > > H&E staining of the slide. I also understand that facilities might > > > have different descriptions, but what I'm really looking for is > > > what CAP > > allows > > > them to do. > > > > > > Thanks for your help, > > > > > > Deloris Carter, HT(ASCP) > > > SMMC > > > Shawnee, KS > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu< mailto:Histonet@lists.utsouthwestern.edu> > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu< mailto:Histonet@lists.utsouthwestern.edu> > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu< mailto:Histonet@lists.utsouthwestern.edu> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ---------------------------------------------------------------------- > Confidentiality Notice: This e-mail message, including any > attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ From TJohnson <@t> gnf.org Wed Sep 11 11:50:36 2013 From: TJohnson <@t> gnf.org (Teri Johnson) Date: Wed Sep 11 11:50:47 2013 Subject: [Histonet] Re: edge effect IHC Message-ID: <9F3CFEE76E51B64991C7485270890B40497C1ACF@EX5.lj.gnf.org> Ditto to what Tim said (as usual!) What is often referred to as "edge artifact" is sometimes excellent staining due to the edges fixed early and well, and the interior of the tissue poorly penetrated and not as well fixed. You can usually tell because the staining pattern is correct and well demarcated, and easily distinguishable from the type seen in drying artifact. Teri Johnson Manager, Histology Genomics Institute for Novartis Research Foundation San Diego, CA 858-332-4752 From relia1 <@t> earthlink.net Wed Sep 11 11:54:09 2013 From: relia1 <@t> earthlink.net (Pam Barker) Date: Wed Sep 11 11:54:13 2013 Subject: [Histonet] RELIA Histology Careers Weekly UPdate 9-11-2013 Message-ID: <019f01ceaf0f$88fa7a50$9aef6ef0$@earthlink.net> Hi Histonetters!!! Do you know what day it is (picture big hairy camel here!) ?????? I think you do! LOLOL I have a couple of great new opportunities to share. If you or anyone you know is interested in more details I can be reached toll free at 866-607-3542 or relia1@earthlink.net. If you are starting to save up your Christmas money remember I pay a referral fee to you if I place someone that you refer to me. Here are the newest opportunities: Histology Supervisor - Tallahassee, FL Night Shift Histotech - Austin, TX Night Shift Grossing Tech - Austin, TX Lead Pathology Tech - Zanesville, OH Histotech - Harrisonburg, VA Histotech - Tyler, TX Thanks and have a great day!! Pam Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From fbozkurt <@t> gmail.com Wed Sep 11 12:05:23 2013 From: fbozkurt <@t> gmail.com (Mehmet Fatih BOZKURT) Date: Wed Sep 11 12:05:35 2013 Subject: [Histonet] Re: edge effect IHC In-Reply-To: <9F3CFEE76E51B64991C7485270890B40497C1ACF@EX5.lj.gnf.org> References: <9F3CFEE76E51B64991C7485270890B40497C1ACF@EX5.lj.gnf.org> Message-ID: Sometimes edges of tissues can get more stain due to disruption of edge's cells more than center during the antigen retrieval. But this is not false positivity. It must pay attention to this situation On Wed, Sep 11, 2013 at 7:50 PM, Teri Johnson wrote: > Ditto to what Tim said (as usual!) What is often referred to as "edge > artifact" is sometimes excellent staining due to the edges fixed early and > well, and the interior of the tissue poorly penetrated and not as well > fixed. You can usually tell because the staining pattern is correct and > well demarcated, and easily distinguishable from the type seen in drying > artifact. > > Teri Johnson > Manager, Histology > Genomics Institute for > Novartis Research > Foundation > San Diego, CA > 858-332-4752 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Mehmet Fatih BOZKURT, DVM, PhD Afyon Kocatepe University Faculty of Veterinary Medicine Department of Pathology 03030, ANS Campus Afyonkarahisar-TURKEY Tel: +902722281312-16173/16237 From rjbuesa <@t> yahoo.com Wed Sep 11 12:15:21 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Sep 11 12:15:30 2013 Subject: [Histonet] Unregistered HT In-Reply-To: References: <484323625.958457.1378907135764.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Message-ID: <1378919721.61659.YahooMailNeo@web163105.mail.bf1.yahoo.com> This very long thread deals with a very complicated and ages long issue so I would like to add my opinion. The fundamental issue is that the pathologists do not respect the histotechs because for them the only thing that matters is that the sections are good, well stained and finished on time. That is all! If they can get some well trained chimpanzees doing these tasks they would be OK with that and they do not give a dam about how much we make or what we know as long as the sections are goo, well stained and on time. Sometimes they decide to "do something" is a histotech completely sick and tired of being disrespected threatens to leave to other lab. The other factor against the histotechs are the managers that prefer to pay the least amount possible and see a histotech with higher education as a potential "money pit" for their budget because they will have to pay them more. Additionally some histotech with higher education are not the best from the quality results point of view and perhaps those with more experience and quality of work are those old histotechs with 20 or more years of experience that usually have been grandfathered and some not even graduated from high school. When I started in this trade (1952) I remember that I was in pre-medical year and learning how to do the basics (embed, section, stain) with the hope of being contracted at the "wonderful" salary of $30/month but that was not to be because the professor head of the department gave the position to a cousin of him and I was supposed to train her, something that did not occur because I left and he had to start all over again. Hiring a janitor or a?cleaning lady to do histology work was not an infrequent occurrence in the mid 1950's and even 30 years later. Why? Again because what the pathologists wanted out of the histotech i.e. good sections the cheapest the better. How this can be solved? It has to start with the pathologists and the administrators, and also with the histotech. We need to demand respect and I coincide that the NSH has done little in that respect. The issue is not only to study and become more knowledgeable in our trade, that is of paramount importance to do a better job, to be able to understand the procedures and be able to trouble shoot but unfortunately many histotechs see this trade as "a decent way or earning a salary" and that is all. Two final caveats: not all pathologists?are born equal, neither all?administrators. Ren? J.? ________________________________ From Jessica.Vacca <@t> HCAhealthcare.com Wed Sep 11 12:19:42 2013 From: Jessica.Vacca <@t> HCAhealthcare.com (Jessica.Vacca@HCAhealthcare.com) Date: Wed Sep 11 12:19:52 2013 Subject: [Histonet] (no subject) In-Reply-To: <9215BD4B0BA1B44D962A71C758B68D2E01882970@HHCEXCHMB05.hhcsystem.org> References: <9215BD4B0BA1B44D962A71C758B68D2E01882970@HHCEXCHMB05.hhcsystem.org> Message-ID: <938D716CD445614ABBB817517557B6F407F1088ACE@NADCWPMSGCMS09.hca.corpad.net> Ask for a recut of slides unstained to be sent for 2nd opinion to your lab or as a patient request unstained slides. I think the billing is the ultimate issue as well a chain of custody for specimens removed-When working at a hospital level the hospital labs have contracted and listed prices for certain reference labs. Usually the reference lab ends up billing the hospital for something that was not submitted from them and there is where the problem lies. You can have the slides sent by you with a method of payment submitted for the consult fee or additional tests. Also the pathologist may have a contract that says specimen collected at the hospital is there's to read and at the time of surgery your dad may have signed something in regards to okaying that a separate bill from the pathology department may be expected should the insurance not cover it. Just a couple of thoughts -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cartun, Richard Sent: Wednesday, September 11, 2013 11:14 AM To: clay milks; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] (no subject) Hospital "By-Laws" established by the Medical Staff and Administration may prevent tissue obtained within the hospital from going outside to another laboratory. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax richard.cartun@hhchealth.org ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of clay milks [claymilks@hotmail.com] Sent: Thursday, September 12, 2013 8:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) I need a little help on a Patient's rights question. It is my understanding that when a patient has a procedure, the patient has the right to request that those specimens be examined by a laboratory of their choosing. i.e. EGD, colonoscopy, etc I am in Arkansas. My father had a procedure yesterday at a local hospital. I manage a pathology laboratory that specializes in the type of tissue that the procedure procured. When it was requested that the tissue be sent to my laboratory, the hospital staff refused to fulfill the request. We asked multiple times for a release form so my father could take his tissue with him. The administration employee we spoke to said there was no such thing and that patients were not allowed to take their specimens. One employee going so far as stating that if we wanted the tissue sent somewhere aside from their contracted laboratory, that the procedure would be canceled and my father would have to go somewhere else. My father, not wanting to cause a fuss, let the issue go. Questions: Is it legal for a hospital to require that tissue specimens be sent to a lab they are contracted with? Are there any other actions we could have taken to make our requests be honored? This is not meant to insult the hospital, but to give an explanation of the situation for context in answering the questions. This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CThornton <@t> dahlchase.com Wed Sep 11 12:20:09 2013 From: CThornton <@t> dahlchase.com (Clare Thornton) Date: Wed Sep 11 12:20:16 2013 Subject: [Histonet] Unregistered HT In-Reply-To: References: <41D3A1AF6FEF0643BDC89E0516A6EA32D31581E1@Mail2Node2.ad.uams.edu> <41D3A1AF6FEF0643BDC89E0516A6EA32D3158371@Mail2Node2.ad.uams.edu> Message-ID: My apologies, the editorial was in the September 2011 issue of Journal of Histotechnology. I have a pdf of it, will be happy to email. Clare J. Thornton, HTL(ASCP)QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Clare Thornton Sent: Wednesday, September 11, 2013 10:20 AM To: 'Emily Sours' Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT Look for a position paper presented by members of the NSH BOD in the Journal of Histotechnology after the national convention in Vancouver last year. I'm not sure of the exact issue (thought I still had it on my desk). This will explain what being a laboratory professional is and why it is so important that as histotechnologists we are viewed as such. Clare J. Thornton, HTL(ASCP)QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Wednesday, September 11, 2013 9:58 AM Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Unregistered HT Do you get paid more with that title? Just curious, I'm not sure what "laboratory professional" means, as I'm not in that kind of lab. Or is it a respect thing? Emily "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." -Chuck Palahniuk, "Haunted" On Tue, Sep 10, 2013 at 7:32 PM, Jennifer MacDonald wrote: > As long as we do not need certification, licensure and minium > education requirements we will not be recognized as Laboratory Professionals. > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Sep 11 12:30:15 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Sep 11 12:30:21 2013 Subject: [Histonet] Unregistered HT In-Reply-To: References: <41D3A1AF6FEF0643BDC89E0516A6EA32D31581E1@Mail2Node2.ad.uams.edu> <41D3A1AF6FEF0643BDC89E0516A6EA32D3158371@Mail2Node2.ad.uams.edu> Message-ID: <1378920615.79308.YahooMailNeo@web163104.mail.bf1.yahoo.com> The issue remains the WE cannot impose on others how we are viewed. No matter how many brain storming meetings there are about "demanding" respect the issue remains that those who have to respect us have to do so. Short of a national strike (absolutely impossible to achieve) or a national union (that will be fought to the death) the only thing we can do is to stand for our rights of being treated as professionals every time somebody, either pathologist or administrator, disrespects us. And who, may I ask, will be able to do that in an economic situation as the present one? Who is going to risk his or her job just "to demand respect"? That is an illusion. Unfortunately the economic situation impedes any action and those who can will keep disrespecting us as long as they can get away with it. Having a good "position statement" about "what does it mean to be a professional" is an exercise in futility that probably for those who wrote it may have?meant being able to have 1 or 2 days paid without actual work to do. Ren? J. ________________________________ From: Clare Thornton To: Clare Thornton ; 'Emily Sours' Cc: "histonet@lists.utsouthwestern.edu" Sent: Wednesday, September 11, 2013 1:20 PM Subject: RE: [Histonet] Unregistered HT My apologies, the editorial was in the September 2011 issue of Journal of Histotechnology.? I have a pdf of it, will be happy to email. Clare J. Thornton, HTL(ASCP)QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Clare Thornton Sent: Wednesday, September 11, 2013 10:20 AM To: 'Emily Sours' Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT Look for a position paper presented by members of the NSH BOD in the Journal of Histotechnology after the national convention in Vancouver last year.? I'm not sure of the exact issue (thought I still had it on my desk).? This will explain what being a laboratory professional is and why it is so important that as histotechnologists we are viewed as such. Clare J. Thornton, HTL(ASCP)QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Wednesday, September 11, 2013 9:58 AM Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Unregistered HT Do you get paid more with that title? Just curious, I'm not sure what "laboratory professional" means, as I'm not in that kind of lab. Or is it a respect thing? Emily "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." -Chuck Palahniuk, "Haunted" On Tue, Sep 10, 2013 at 7:32 PM, Jennifer MacDonald wrote: > As long as we do not need certification, licensure and minium > education requirements we will not be recognized as Laboratory Professionals. > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lcolbert <@t> pathmdlabs.com Wed Sep 11 12:45:43 2013 From: lcolbert <@t> pathmdlabs.com (Laurie Colbert) Date: Wed Sep 11 12:49:21 2013 Subject: [Histonet] Validation Sign off Message-ID: <12ECD7346266D74691EC2BFC75285E452F33E9F1@BFL323E10.pathmdlabs.local> When validating IHC antibodies and detection kits (either initially or with new lots), does a pathologist have to sign off on the validation , or can it be his/her designee (such as a PhD) that signs off? The pathologist will be signing the IHC procedures. Laurie Colbert, HT (ASCP) From Timothy.Morken <@t> ucsfmedctr.org Wed Sep 11 13:08:39 2013 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Wed Sep 11 13:08:57 2013 Subject: [Histonet] RE: Validation Sign off In-Reply-To: <12ECD7346266D74691EC2BFC75285E452F33E9F1@BFL323E10.pathmdlabs.local> References: <12ECD7346266D74691EC2BFC75285E452F33E9F1@BFL323E10.pathmdlabs.local> Message-ID: <761E2B5697F795489C8710BCC72141FF0993E3@ex07.net.ucsf.edu> Laurie, it can be a designee, but that should be detailed in your validation procedure so it is clear the designee is acting for the Technical Director. Tim Morken Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Wednesday, September 11, 2013 10:46 AM To: Histonet Post (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Validation Sign off When validating IHC antibodies and detection kits (either initially or with new lots), does a pathologist have to sign off on the validation , or can it be his/her designee (such as a PhD) that signs off? The pathologist will be signing the IHC procedures. Laurie Colbert, HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek <@t> digestivespecialists.com Wed Sep 11 13:15:27 2013 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Wed Sep 11 13:15:32 2013 Subject: [Histonet] Unregistered HT In-Reply-To: <1378920615.79308.YahooMailNeo@web163104.mail.bf1.yahoo.com> References: <41D3A1AF6FEF0643BDC89E0516A6EA32D31581E1@Mail2Node2.ad.uams.edu> <41D3A1AF6FEF0643BDC89E0516A6EA32D3158371@Mail2Node2.ad.uams.edu> <1378920615.79308.YahooMailNeo@web163104.mail.bf1.yahoo.com> Message-ID: <5A2BD13465E061429D6455C8D6B40E39165E330DA5@IBMB7Exchange.digestivespecialists.com> Wow Ren?, I didn't realize you had such a demeaning and disrespected career. I have been in this profession for almost 40 years. I have worked for some of the greatest pathologists you could ask for. They have respected me, asked my opinions and shared a great deal of time at the microscope with me. Maybe it has to do with attitude...... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, September 11, 2013 1:30 PM To: Clare Thornton; 'Emily Sours' Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Unregistered HT The issue remains the WE cannot impose on others how we are viewed. No matter how many brain storming meetings there are about "demanding" respect the issue remains that those who have to respect us have to do so. Short of a national strike (absolutely impossible to achieve) or a national union (that will be fought to the death) the only thing we can do is to stand for our rights of being treated as professionals every time somebody, either pathologist or administrator, disrespects us. And who, may I ask, will be able to do that in an economic situation as the present one? Who is going to risk his or her job just "to demand respect"? That is an illusion. Unfortunately the economic situation impedes any action and those who can will keep disrespecting us as long as they can get away with it. Having a good "position statement" about "what does it mean to be a professional" is an exercise in futility that probably for those who wrote it may have?meant being able to have 1 or 2 days paid without actual work to do. Ren? J. ________________________________ From: Clare Thornton To: Clare Thornton ; 'Emily Sours' Cc: "histonet@lists.utsouthwestern.edu" Sent: Wednesday, September 11, 2013 1:20 PM Subject: RE: [Histonet] Unregistered HT My apologies, the editorial was in the September 2011 issue of Journal of Histotechnology.? I have a pdf of it, will be happy to email. Clare J. Thornton, HTL(ASCP)QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Clare Thornton Sent: Wednesday, September 11, 2013 10:20 AM To: 'Emily Sours' Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT Look for a position paper presented by members of the NSH BOD in the Journal of Histotechnology after the national convention in Vancouver last year.? I'm not sure of the exact issue (thought I still had it on my desk).? This will explain what being a laboratory professional is and why it is so important that as histotechnologists we are viewed as such. Clare J. Thornton, HTL(ASCP)QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Wednesday, September 11, 2013 9:58 AM Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Unregistered HT Do you get paid more with that title? Just curious, I'm not sure what "laboratory professional" means, as I'm not in that kind of lab. Or is it a respect thing? Emily "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." -Chuck Palahniuk, "Haunted" On Tue, Sep 10, 2013 at 7:32 PM, Jennifer MacDonald wrote: > As long as we do not need certification, licensure and minium > education requirements we will not be recognized as Laboratory Professionals. > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sdysart <@t> mirnarx.com Wed Sep 11 13:30:15 2013 From: sdysart <@t> mirnarx.com (Sarah Dysart) Date: Wed Sep 11 13:30:25 2013 Subject: [Histonet] Unregistered HT In-Reply-To: <5A2BD13465E061429D6455C8D6B40E39165E330DA5@IBMB7Exchange.digestivespecialists.com> References: <41D3A1AF6FEF0643BDC89E0516A6EA32D31581E1@Mail2Node2.ad.uams.edu> <41D3A1AF6FEF0643BDC89E0516A6EA32D3158371@Mail2Node2.ad.uams.edu> <1378920615.79308.YahooMailNeo@web163104.mail.bf1.yahoo.com> <5A2BD13465E061429D6455C8D6B40E39165E330DA5@IBMB7Exchange.digestivespecialists.com> Message-ID: I don't think that histotechs are disrespected at all. There is always going to be that one person in any job that thinks they are better than you because of whatever reason. HTs are not the highest paid people in the lab (or the lowest for that matter), and I think a lot of times people in management, PhD, or MD positions see us in a light that mirrors our salaries. The fact of the matter is these days you have to basically have a college degree to become a HT, and hopefully as the "old-timers" who didn't have to have a degree retire out of the field we will be paid more according to the education that a lot of us paid so much money to obtain. I still hold firm to the belief that you get what you give. If you act like someone who should be disrespected you will be. If you suggest new ideas for improvement, go over and beyond your "pay grade", then eventually you will be paid for that financially and with respect. If I couldn't live like that I think that would be a very difficult and frustrating way to live every day. Therefore, I choose to have a good attitude about being away from my family everyday (because I have to have a job), and do the best I can to make others have a reason to respect me. Just my two cents...I will step down from my soapbox now =) Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Wednesday, September 11, 2013 1:15 PM To: Rene J Buesa; Clare Thornton; 'Emily Sours' Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT Wow Ren?, I didn't realize you had such a demeaning and disrespected career. I have been in this profession for almost 40 years. I have worked for some of the greatest pathologists you could ask for. They have respected me, asked my opinions and shared a great deal of time at the microscope with me. Maybe it has to do with attitude...... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, September 11, 2013 1:30 PM To: Clare Thornton; 'Emily Sours' Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Unregistered HT The issue remains the WE cannot impose on others how we are viewed. No matter how many brain storming meetings there are about "demanding" respect the issue remains that those who have to respect us have to do so. Short of a national strike (absolutely impossible to achieve) or a national union (that will be fought to the death) the only thing we can do is to stand for our rights of being treated as professionals every time somebody, either pathologist or administrator, disrespects us. And who, may I ask, will be able to do that in an economic situation as the present one? Who is going to risk his or her job just "to demand respect"? That is an illusion. Unfortunately the economic situation impedes any action and those who can will keep disrespecting us as long as they can get away with it. Having a good "position statement" about "what does it mean to be a professional" is an exercise in futility that probably for those who wrote it may have?meant being able to have 1 or 2 days paid without actual work to do. Ren? J. ________________________________ From: Clare Thornton To: Clare Thornton ; 'Emily Sours' Cc: "histonet@lists.utsouthwestern.edu" Sent: Wednesday, September 11, 2013 1:20 PM Subject: RE: [Histonet] Unregistered HT My apologies, the editorial was in the September 2011 issue of Journal of Histotechnology.? I have a pdf of it, will be happy to email. Clare J. Thornton, HTL(ASCP)QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Clare Thornton Sent: Wednesday, September 11, 2013 10:20 AM To: 'Emily Sours' Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT Look for a position paper presented by members of the NSH BOD in the Journal of Histotechnology after the national convention in Vancouver last year.? I'm not sure of the exact issue (thought I still had it on my desk).? This will explain what being a laboratory professional is and why it is so important that as histotechnologists we are viewed as such. Clare J. Thornton, HTL(ASCP)QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Wednesday, September 11, 2013 9:58 AM Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Unregistered HT Do you get paid more with that title? Just curious, I'm not sure what "laboratory professional" means, as I'm not in that kind of lab. Or is it a respect thing? Emily "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." -Chuck Palahniuk, "Haunted" On Tue, Sep 10, 2013 at 7:32 PM, Jennifer MacDonald wrote: > As long as we do not need certification, licensure and minium > education requirements we will not be recognized as Laboratory Professionals. > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Donna.Willis <@t> baylorhealth.edu Wed Sep 11 13:30:52 2013 From: Donna.Willis <@t> baylorhealth.edu (Willis, Donna G.) Date: Wed Sep 11 13:30:56 2013 Subject: [Histonet] RE: Validation Sign off In-Reply-To: <761E2B5697F795489C8710BCC72141FF0993E3@ex07.net.ucsf.edu> References: <12ECD7346266D74691EC2BFC75285E452F33E9F1@BFL323E10.pathmdlabs.local> <761E2B5697F795489C8710BCC72141FF0993E3@ex07.net.ucsf.edu> Message-ID: <2572B4D63B62E64A8078D8BBE34D4078338550@BHDASVEXML2.bhcs.pvt> Laure, You also need to have a policy that states what areas the CLIA Director is allowing a designee to represent. Donna Willis, HT/HTL (ASCP) Anatomic Pathology Manager Baylor University Medical Center-Dallas ph. 214-820-2465 office ph. 214-725-6184 mobile donna.willis@baylorhealth.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Wednesday, September 11, 2013 1:09 PM To: Laurie Colbert; Histonet Post (histonet@lists.utsouthwestern.edu) Subject: [Histonet] RE: Validation Sign off Laurie, it can be a designee, but that should be detailed in your validation procedure so it is clear the designee is acting for the Technical Director. Tim Morken Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Wednesday, September 11, 2013 10:46 AM To: Histonet Post (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Validation Sign off When validating IHC antibodies and detection kits (either initially or with new lots), does a pathologist have to sign off on the validation , or can it be his/her designee (such as a PhD) that signs off? The pathologist will be signing the IHC procedures. Laurie Colbert, HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This e-mail may contain confidential and/or privileged information. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. From mw <@t> personifysearch.com Wed Sep 11 13:38:36 2013 From: mw <@t> personifysearch.com (Matt Ward) Date: Wed Sep 11 13:38:40 2013 Subject: [Histonet] Field Histology Specialist - West Coast Message-ID: <718ea7d18c167e142d4aff21196fdea8@mail.gmail.com> Good afternoon! We have had a new opportunity open with a world leading manufacturer of histology products. Our client is growing and searching for a histology professional to join their team on the West Coast. The ideal candidate will be based in CA, and will work in the field with sales reps as the technical specialist. This opportunity is perfect for someone who is looking to break out of the laboratory and into the field. The opportunity offers a competitive base salary + bonus + great benefits. If this sounds like something you would be interested in learning more about, please contact me at mw@personifysearch.com, or call 800.875.6188 ext. 103. I look forward to connecting soon! Best Regards, Matt Ward *Account Executive* *Personify* 5020 Weston Parkway Suite 315 Cary NC 27513 (Tel) 800.875.6188 direct ext 103 (Fax) 919.460.0642 www.personifysearch.com From PAMarcum <@t> uams.edu Wed Sep 11 13:57:22 2013 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Wed Sep 11 13:57:47 2013 Subject: [Histonet] Unregistered HT In-Reply-To: References: <41D3A1AF6FEF0643BDC89E0516A6EA32D31581E1@Mail2Node2.ad.uams.edu> <41D3A1AF6FEF0643BDC89E0516A6EA32D3158371@Mail2Node2.ad.uams.edu> <1378920615.79308.YahooMailNeo@web163104.mail.bf1.yahoo.com> <5A2BD13465E061429D6455C8D6B40E39165E330DA5@IBMB7Exchange.digestivespecialists.com> Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA32D31586ED@Mail2Node2.ad.uams.edu> Sorry Sarah some of us old timers do have BS and even MS degrees and still have to fight for every dime. The whole field needs to be improved without any thought of how long someone has been in Histology. We have the issue of people who have HTs being called HTLs due to the way the two registries were created. In the early 1980s the only thing you could get was the HT registry no matter how much education you had worked to get. Then we got the HTL for management skills as it was stated and for several years HTs could grandfather in with no additional education above HS. Then in about 1984 the HTL started to require the education level of BS. Unfortunately, since the two registries were to some extend mixed by the overlap of needing or not needing a degree the whole field is confused about who is what. I have been called a histologist before I took my HT (with a BS) and HTL although I did not bother to grandfather in. Yet I am called both and really don't care which I am called as long as I am respected. An MS made no difference either way. The real issue is most administration people and HR Departments don't know the difference between an HT and HTL and have no idea what to do. I have seen HTs with a HS call themselves HTLs and no one knew the difference at the administration/HR levels. (Some of those older people who started and may not even have a HS degree yet; are among the best histologist I have seen in 50 years. Some with BS and/or MS degrees were terrible. Some MTs came over with CP pay and could be great or awful.) Education is not answer. The issue is straightening out who we are and what we do as either registry area. Here we had a salary survey and found most of the employers just paid the same for everything to avoid the issue or due to total misunderstanding of their even being a difference. Now everyone is the same. No one knew how to solve it to everyone's satisfaction and the histologist were not asked. If you can say you have a BS you may get paid a little higher and only have an HT and be called an HTL. Pam Marcum -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sarah Dysart Sent: Wednesday, September 11, 2013 1:30 PM To: Blazek, Linda; Rene J Buesa; Clare Thornton; 'Emily Sours' Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT I don't think that histotechs are disrespected at all. There is always going to be that one person in any job that thinks they are better than you because of whatever reason. HTs are not the highest paid people in the lab (or the lowest for that matter), and I think a lot of times people in management, PhD, or MD positions see us in a light that mirrors our salaries. The fact of the matter is these days you have to basically have a college degree to become a HT, and hopefully as the "old-timers" who didn't have to have a degree retire out of the field we will be paid more according to the education that a lot of us paid so much money to obtain. I still hold firm to the belief that you get what you give. If you act like someone who should be disrespected you will be. If you suggest new ideas for improvement, go over and beyond your "pay grade", then eventually you will be paid for that financially and with respect. If I couldn't live like that I think that would be a very difficult and frustrating way to live every day. Therefore, I choose to have a good attitude about being away from my family everyday (because I have to have a job), and do the best I can to make others have a reason to respect me. Just my two cents...I will step down from my soapbox now =) Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Wednesday, September 11, 2013 1:15 PM To: Rene J Buesa; Clare Thornton; 'Emily Sours' Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT Wow Ren?, I didn't realize you had such a demeaning and disrespected career. I have been in this profession for almost 40 years. I have worked for some of the greatest pathologists you could ask for. They have respected me, asked my opinions and shared a great deal of time at the microscope with me. Maybe it has to do with attitude...... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, September 11, 2013 1:30 PM To: Clare Thornton; 'Emily Sours' Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Unregistered HT The issue remains the WE cannot impose on others how we are viewed. No matter how many brain storming meetings there are about "demanding" respect the issue remains that those who have to respect us have to do so. Short of a national strike (absolutely impossible to achieve) or a national union (that will be fought to the death) the only thing we can do is to stand for our rights of being treated as professionals every time somebody, either pathologist or administrator, disrespects us. And who, may I ask, will be able to do that in an economic situation as the present one? Who is going to risk his or her job just "to demand respect"? That is an illusion. Unfortunately the economic situation impedes any action and those who can will keep disrespecting us as long as they can get away with it. Having a good "position statement" about "what does it mean to be a professional" is an exercise in futility that probably for those who wrote it may have?meant being able to have 1 or 2 days paid without actual work to do. Ren? J. ________________________________ From: Clare Thornton To: Clare Thornton ; 'Emily Sours' Cc: "histonet@lists.utsouthwestern.edu" Sent: Wednesday, September 11, 2013 1:20 PM Subject: RE: [Histonet] Unregistered HT My apologies, the editorial was in the September 2011 issue of Journal of Histotechnology.? I have a pdf of it, will be happy to email. Clare J. Thornton, HTL(ASCP)QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Clare Thornton Sent: Wednesday, September 11, 2013 10:20 AM To: 'Emily Sours' Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT Look for a position paper presented by members of the NSH BOD in the Journal of Histotechnology after the national convention in Vancouver last year.? I'm not sure of the exact issue (thought I still had it on my desk).? This will explain what being a laboratory professional is and why it is so important that as histotechnologists we are viewed as such. Clare J. Thornton, HTL(ASCP)QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Wednesday, September 11, 2013 9:58 AM Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Unregistered HT Do you get paid more with that title? Just curious, I'm not sure what "laboratory professional" means, as I'm not in that kind of lab. Or is it a respect thing? Emily "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." -Chuck Palahniuk, "Haunted" On Tue, Sep 10, 2013 at 7:32 PM, Jennifer MacDonald wrote: > As long as we do not need certification, licensure and minium > education requirements we will not be recognized as Laboratory Professionals. > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From sdysart <@t> mirnarx.com Wed Sep 11 14:07:34 2013 From: sdysart <@t> mirnarx.com (Sarah Dysart) Date: Wed Sep 11 14:07:50 2013 Subject: [Histonet] Unregistered HT In-Reply-To: <41D3A1AF6FEF0643BDC89E0516A6EA32D31586ED@Mail2Node2.ad.uams.edu> References: <41D3A1AF6FEF0643BDC89E0516A6EA32D31581E1@Mail2Node2.ad.uams.edu> <41D3A1AF6FEF0643BDC89E0516A6EA32D3158371@Mail2Node2.ad.uams.edu> <1378920615.79308.YahooMailNeo@web163104.mail.bf1.yahoo.com> <5A2BD13465E061429D6455C8D6B40E39165E330DA5@IBMB7Exchange.digestivespecialists.com> <41D3A1AF6FEF0643BDC89E0516A6EA32D31586ED@Mail2Node2.ad.uams.edu> Message-ID: <055f378f3add412a8a95c34a241bb988@BY2PR07MB106.namprd07.prod.outlook.com> So as not to have my inbox completely filled with emails... None of the below statements was meant in any way as disrespectful to ANYONE! 1. "Old timers". Just a term I have heard used by people who have been in the field for a long time. I've been doing histology since 1999...I do not consider myself one of these yet. 2. A degree. I am not saying that all "old timers" are without a degree or any post HS education. Correct, many non-degreed workers are better than a degreed one. I just think someone with a college degree should be paid accordingly. If they are horrible at their job...they will probably be fired. If you didn't finish your degree and still want to...go do it. 3. Chill out people...it's Wednesday...sheesh... Some of the emails I have got offline completely demonstrate my point...give respect and you will be respected. If you send me a tacky email...I'm probably going to be tacky right back to you... 4. These were all just my opinions...take it or leave it. I hope everyone has a great rest of your day, and some people I think need to go home and have a drink =) Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: Marcum, Pamela A [mailto:PAMarcum@uams.edu] Sent: Wednesday, September 11, 2013 1:57 PM To: Sarah Dysart; Blazek, Linda; Rene J Buesa; Clare Thornton; 'Emily Sours' Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT Sorry Sarah some of us old timers do have BS and even MS degrees and still have to fight for every dime. The whole field needs to be improved without any thought of how long someone has been in Histology. We have the issue of people who have HTs being called HTLs due to the way the two registries were created. In the early 1980s the only thing you could get was the HT registry no matter how much education you had worked to get. Then we got the HTL for management skills as it was stated and for several years HTs could grandfather in with no additional education above HS. Then in about 1984 the HTL started to require the education level of BS. Unfortunately, since the two registries were to some extend mixed by the overlap of needing or not needing a degree the whole field is confused about who is what. I have been called a histologist before I took my HT (with a BS) and HTL although I did not bother to grandfather in. Yet I am called both and really don't care which I am called as long as I am respected. An MS made no difference either way. The real issue is most administration people and HR Departments don't know the difference between an HT and HTL and have no idea what to do. I have seen HTs with a HS call themselves HTLs and no one knew the difference at the administration/HR levels. (Some of those older people who started and may not even have a HS degree yet; are among the best histologist I have seen in 50 years. Some with BS and/or MS degrees were terrible. Some MTs came over with CP pay and could be great or awful.) Education is not answer. The issue is straightening out who we are and what we do as either registry area. Here we had a salary survey and found most of the employers just paid the same for everything to avoid the issue or due to total misunderstanding of their even being a difference. Now everyone is the same. No one knew how to solve it to everyone's satisfaction and the histologist were not asked. If you can say you have a BS you may get paid a little higher and only have an HT and be called an HTL. Pam Marcum -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sarah Dysart Sent: Wednesday, September 11, 2013 1:30 PM To: Blazek, Linda; Rene J Buesa; Clare Thornton; 'Emily Sours' Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT I don't think that histotechs are disrespected at all. There is always going to be that one person in any job that thinks they are better than you because of whatever reason. HTs are not the highest paid people in the lab (or the lowest for that matter), and I think a lot of times people in management, PhD, or MD positions see us in a light that mirrors our salaries. The fact of the matter is these days you have to basically have a college degree to become a HT, and hopefully as the "old-timers" who didn't have to have a degree retire out of the field we will be paid more according to the education that a lot of us paid so much money to obtain. I still hold firm to the belief that you get what you give. If you act like someone who should be disrespected you will be. If you suggest new ideas for improvement, go over and beyond your "pay grade", then eventually you will be paid for that financially and with respect. If I couldn't live like that I think that would be a very difficult and frustrating way to live every day. Therefore, I choose to have a good attitude about being away from my family everyday (because I have to have a job), and do the best I can to make others have a reason to respect me. Just my two cents...I will step down from my soapbox now =) Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Wednesday, September 11, 2013 1:15 PM To: Rene J Buesa; Clare Thornton; 'Emily Sours' Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT Wow Ren?, I didn't realize you had such a demeaning and disrespected career. I have been in this profession for almost 40 years. I have worked for some of the greatest pathologists you could ask for. They have respected me, asked my opinions and shared a great deal of time at the microscope with me. Maybe it has to do with attitude...... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, September 11, 2013 1:30 PM To: Clare Thornton; 'Emily Sours' Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Unregistered HT The issue remains the WE cannot impose on others how we are viewed. No matter how many brain storming meetings there are about "demanding" respect the issue remains that those who have to respect us have to do so. Short of a national strike (absolutely impossible to achieve) or a national union (that will be fought to the death) the only thing we can do is to stand for our rights of being treated as professionals every time somebody, either pathologist or administrator, disrespects us. And who, may I ask, will be able to do that in an economic situation as the present one? Who is going to risk his or her job just "to demand respect"? That is an illusion. Unfortunately the economic situation impedes any action and those who can will keep disrespecting us as long as they can get away with it. Having a good "position statement" about "what does it mean to be a professional" is an exercise in futility that probably for those who wrote it may have?meant being able to have 1 or 2 days paid without actual work to do. Ren? J. ________________________________ From: Clare Thornton To: Clare Thornton ; 'Emily Sours' Cc: "histonet@lists.utsouthwestern.edu" Sent: Wednesday, September 11, 2013 1:20 PM Subject: RE: [Histonet] Unregistered HT My apologies, the editorial was in the September 2011 issue of Journal of Histotechnology.? I have a pdf of it, will be happy to email. Clare J. Thornton, HTL(ASCP)QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Clare Thornton Sent: Wednesday, September 11, 2013 10:20 AM To: 'Emily Sours' Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT Look for a position paper presented by members of the NSH BOD in the Journal of Histotechnology after the national convention in Vancouver last year.? I'm not sure of the exact issue (thought I still had it on my desk).? This will explain what being a laboratory professional is and why it is so important that as histotechnologists we are viewed as such. Clare J. Thornton, HTL(ASCP)QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Wednesday, September 11, 2013 9:58 AM Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Unregistered HT Do you get paid more with that title? Just curious, I'm not sure what "laboratory professional" means, as I'm not in that kind of lab. Or is it a respect thing? Emily "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." -Chuck Palahniuk, "Haunted" On Tue, Sep 10, 2013 at 7:32 PM, Jennifer MacDonald wrote: > As long as we do not need certification, licensure and minium > education requirements we will not be recognized as Laboratory Professionals. > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From talulahgosh <@t> gmail.com Wed Sep 11 14:24:28 2013 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Wed Sep 11 14:24:34 2013 Subject: [Histonet] Unregistered HT In-Reply-To: <055f378f3add412a8a95c34a241bb988@BY2PR07MB106.namprd07.prod.outlook.com> References: <41D3A1AF6FEF0643BDC89E0516A6EA32D31581E1@Mail2Node2.ad.uams.edu> <41D3A1AF6FEF0643BDC89E0516A6EA32D3158371@Mail2Node2.ad.uams.edu> <1378920615.79308.YahooMailNeo@web163104.mail.bf1.yahoo.com> <5A2BD13465E061429D6455C8D6B40E39165E330DA5@IBMB7Exchange.digestivespecialists.com> <41D3A1AF6FEF0643BDC89E0516A6EA32D31586ED@Mail2Node2.ad.uams.edu> <055f378f3add412a8a95c34a241bb988@BY2PR07MB106.namprd07.prod.outlook.com> Message-ID: Seriously, I can go home?! I'm going to tell my boss that Sarah Dysart said I could go home. When she asks who that is, I'll just look at her and repeat your name. Slowly. And walk away. Emily "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." -Chuck Palahniuk, "Haunted" On Wed, Sep 11, 2013 at 3:07 PM, Sarah Dysart wrote: > > I hope everyone has a great rest of your day, and some people I think need > to go home and have a drink =) > > Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) > Histotechnologist > Mirna Therapeutics > 2150 Woodward Street > Suite 100 > Austin, Texas 78744 > (512)901-0900 ext. 6912 > > > From gu.lang <@t> gmx.at Wed Sep 11 14:27:05 2013 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Wed Sep 11 14:27:12 2013 Subject: AW: [Histonet] Re: edge effect IHC In-Reply-To: References: <9F3CFEE76E51B64991C7485270890B40497C1ACF@EX5.lj.gnf.org> Message-ID: <001301ceaf24$e704d4a0$b50e7de0$@gmx.at> Thank you for your kind responses. Are there any circumstances when an edge-effect could be misinterpreted as a specific nuclear staining like ER-IHC? =false positive staining Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Mehmet Fatih BOZKURT Gesendet: Mittwoch, 11. September 2013 19:05 An: Teri Johnson; Histonet@lists.utsouthwestern.edu Betreff: Re: [Histonet] Re: edge effect IHC Sometimes edges of tissues can get more stain due to disruption of edge's cells more than center during the antigen retrieval. But this is not false positivity. It must pay attention to this situation On Wed, Sep 11, 2013 at 7:50 PM, Teri Johnson wrote: > Ditto to what Tim said (as usual!) What is often referred to as "edge > artifact" is sometimes excellent staining due to the edges fixed early > and well, and the interior of the tissue poorly penetrated and not as > well fixed. You can usually tell because the staining pattern is > correct and well demarcated, and easily distinguishable from the type > seen in drying artifact. > > Teri Johnson > Manager, Histology > Genomics Institute for > Novartis Research > Foundation > San Diego, CA > 858-332-4752 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Mehmet Fatih BOZKURT, DVM, PhD Afyon Kocatepe University Faculty of Veterinary Medicine Department of Pathology 03030, ANS Campus Afyonkarahisar-TURKEY Tel: +902722281312-16173/16237 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From billodonnell <@t> catholichealth.net Wed Sep 11 14:34:56 2013 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Wed Sep 11 14:35:03 2013 Subject: [Histonet] Unregistered HT In-Reply-To: References: <41D3A1AF6FEF0643BDC89E0516A6EA32D31581E1@Mail2Node2.ad.uams.edu> <41D3A1AF6FEF0643BDC89E0516A6EA32D3158371@Mail2Node2.ad.uams.edu> <1378920615.79308.YahooMailNeo@web163104.mail.bf1.yahoo.com> <5A2BD13465E061429D6455C8D6B40E39165E330DA5@IBMB7Exchange.digestivespecialists.com> <41D3A1AF6FEF0643BDC89E0516A6EA32D31586ED@Mail2Node2.ad.uams.edu> <055f378f3add412a8a95c34a241bb988@BY2PR07MB106.namprd07.prod.outlook.com> Message-ID: Sarah & Emily, It's been one of those Wednesdays for the record books, I may not make it all the way home before I have a drink. (Just kidding - I live 8 blocks from work - I can make it home first.... but only if I want too) Ya'll have a great day - - Bill -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Wednesday, September 11, 2013 2:24 PM Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Unregistered HT Seriously, I can go home?! I'm going to tell my boss that Sarah Dysart said I could go home. When she asks who that is, I'll just look at her and repeat your name. Slowly. And walk away. Emily "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." -Chuck Palahniuk, "Haunted" On Wed, Sep 11, 2013 at 3:07 PM, Sarah Dysart wrote: > > I hope everyone has a great rest of your day, and some people I think > need to go home and have a drink =) > > Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna > Therapeutics > 2150 Woodward Street > Suite 100 > Austin, Texas 78744 > (512)901-0900 ext. 6912 > > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. From TJohnson <@t> gnf.org Wed Sep 11 15:14:17 2013 From: TJohnson <@t> gnf.org (Teri Johnson) Date: Wed Sep 11 15:14:22 2013 Subject: [Histonet] Re: Unregistered HT Message-ID: <9F3CFEE76E51B64991C7485270890B40497C1FBD@EX5.lj.gnf.org> At the risk of dipping my toe in to this pool, I have just a few thoughts on the matter. I will not really touch on any prior points as I think they have already had enough discussion. My comments are merely things I think about, and hopefully it broadens how people look at this issue instead of running in circles like we seem to be doing. 1 - I think what will eventually drive the HT classification into a professional category is the upcoming shortage of techs in the workforce. Either the companies better get really busy and come up with some fantastic automation that can replace this aging workforce (of which I am one), or there won't be any bodies sitting at the microtomes, cryostats, embedding centers, and stainers. There are a bunch of BS-biology degreed people out there looking for work and direction. And not all of them can or want to get into Medical school. They are smart, very technologically savvy, and will likely completely change the landscape of the workforce as we know it. 2 - Any move to becoming degreed or certified professionals should mimic the European (and other) model of Laboratory Scientist. I have said before that there is such a strong line dividing MT(ASCP) and HT/HTL(ASCP) in the US, maybe it would be a step in the right direction for us to think about consolidating our science with Medical Technology. We have Chemistry, Hematology, Blood Bank, Microbiology, etc as specialties, why not Histotechnology? Interestingly, most Med Techs I know who have done histology really didn't like it much. So I don't think we have to worry about losing our place in the lab. Do I think we will go in this direction? Probably not. I think that ship has sailed. But we would be remiss not to at least consider it as an option. 3 - "Opportunity looks a lot like hard work." - Ashton Kutcher *donning my special humpday flame retardant suit* Teri Johnson Manager, Histology Genomics Institute for Novartis Research Foundation San Diego, CA 858-332-4752 From tapiarita <@t> yahoo.com Wed Sep 11 15:18:53 2013 From: tapiarita <@t> yahoo.com (tapiarita) Date: Wed Sep 11 15:18:30 2013 Subject: [Histonet] Validation Sign off Message-ID: ?Based on FDA?s ruling on class reagents in IHC, the legal responsibility for validation and knowing the relevant parameters is put squarely on the shoulders of the lab director.? True, the legal responsibility rests with the lab director.. Sent from my Samsung Galaxy S?4 -------- Original message -------- From: Laurie Colbert Date: 09/11/2013 10:45 AM (GMT-07:00) To: "Histonet Post (histonet@lists.utsouthwestern.edu)" Subject: [Histonet] Validation Sign off When validating IHC antibodies and detection kits (either initially or with new lots), does a pathologist have to sign off on the validation , or can it be his/her designee (such as a PhD) that signs off?? The pathologist will be signing the IHC procedures. Laurie Colbert, HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pev <@t> xs4all.nl Wed Sep 11 15:31:12 2013 From: pev <@t> xs4all.nl (P.E. Visser) Date: Wed Sep 11 15:31:00 2013 Subject: [Histonet] Oil red O In-Reply-To: <1378756749.99197.YahooMailNeo@web163104.mail.bf1.yahoo.com> References: <000501cead94$80227530$80675f90$@xs4all.nl> <1378755896.2531.YahooMailNeo@web163105.mail.bf1.yahoo.com> <1378756749.99197.YahooMailNeo@web163104.mail.bf1.yahoo.com> Message-ID: <005c01ceaf2d$dd6d25b0$98477110$@xs4all.nl> Dear Rene, Jennifer and others my question is as Jennifer MacDonald suggested I may agree that the question is irrelevant but the question was rased and I have no clue. Regards Piet visser Van: Rene J Buesa [mailto:rjbuesa@yahoo.com] Verzonden: maandag 9 september 2013 21:59 Aan: Jennifer MacDonald CC: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu; P.E. Visser Onderwerp: Re: [Histonet] Oil red O It is for Sudan Red 5B. All these "apothecary" names are essentially meaningless Ren? J. From: Jennifer MacDonald < JMacDonald@mtsac.edu> To: Cc: " histonet@lists.utsouthwestern.edu" < histonet@lists.utsouthwestern.edu>; histonet-bounces@lists.utsouthwestern.edu; P.E. Visser < pev@xs4all.nl> Sent: Monday, September 9, 2013 3:53 PM Subject: Re: [Histonet] Oil red O I believe the question is what is the "O" signify. as in OG6 the O is for orange. From: Rene J Buesa To: "P.E. Visser" , "histonet@lists.utsouthwestern.edu" Date: 09/09/2013 12:46 PM Subject: Re: [Histonet] Oil red O Sent by: histonet-bounces@lists.utsouthwestern.edu Do you mean that you were asked to do Oil Red O (ORO) stain? It is described in any technology book. I piece of advise: stain the nuclei with hametoxyline first and after that stain with ORO Ren? J. ________________________________ From: P.E. Visser To: histonet@lists.utsouthwestern.edu Sent: Monday, September 9, 2013 3:40 PM Subject: [Histonet] Oil red O Hi all I was requested where the O stands for. who has any suggestion. Regards Piet Visser Histotech Bronovo The Netherlands _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Haley.Huggins <@t> DignityHealth.org Wed Sep 11 15:46:00 2013 From: Haley.Huggins <@t> DignityHealth.org (Huggins, Haley - MRMC) Date: Wed Sep 11 15:46:06 2013 Subject: [Histonet] Equipment selection Message-ID: <4F36EC93A5737D4F8A2974E8FB8E260615F5698BE8@PHX-MSG-007-N2.chw.edu> Hi all, I am trying to decide on what equipment to purchase. I have picked the processor, microtomes, embedding centers and a few other pieces, but now I still need to decide on the H&E Stainer and the immunostainer. My options I have in front of me are for the H&E stainer are Sakura Prisma stainer/film coverslipper and the Leica ST5020-CV5030 stainer/glass coverslipper. For the Immunostainer, I am looking at the Dako Autostainer and the Leica Bond Max. Any advice on any of these instruments would be greatly appreciated. I am trying to finalize my decision pretty quickly. Thank you in advance for any input. From TNMayer <@t> mdanderson.org Wed Sep 11 16:04:02 2013 From: TNMayer <@t> mdanderson.org (Mayer,Toysha N) Date: Wed Sep 11 16:04:07 2013 Subject: [Histonet] RE:Unregistered HT Message-ID: <47E9B2C01DDDD94881EACD2DC44EBC8802B24F2C@D1PWPEXMBX05.mdanderson.edu> I actually agree with Rene on some points. In the past, and in some current labs, that mentality of just get the work done prevails. It happens more in small labs than in larger ones now. However the tide is starting to change. While it may be years before the old regime mentality retires out (we all know that pathologists don't really retire they hang around forever, no offense Samurai pathologist), management is changing. We must demonstrate to ourselves that we matter. Too many places still allow the docs to control the lab and it has hindered us. It has to do with the separation of medicine and business. Not all docs think monkeys can do our job, I know some that understand our value. I don't know what category to place myself in, an old timer or a young buck, I have 20 years in and have paid my dues. I was disrespected, overlooked and scraped floors, but I stayed around. Sometimes I take offense at those who discredit the OJT route, I did it. But I also had a BS degree. I take offense at those who look down on me because I do have a degree, and passed my test. Those were my choices, and I wanted to better position myself for promotion. Some really good techs have no degree, or certification and I would consult with them in a heartbeat. I care about what I do, it matters, so I take pride in my sections and stains. To get our respect we should support our schools (not just because I teach) because they are the ones who can demonstrate the proper skills to students. To do this realize that schools need students to stay open, so send some their way. The faculty cannot teach without them. Be willing to serve as a clinical site to teach what you know, especially if you think they are doing it wrong. Remember that the faculty are under pressure to graduate students so make sure that you support them, don't get mad when they take in more students. We all want to eat. Suggest to your HT's that they go back to get a BS to move up. Facilities are beginning to require supervisors and managers to have that 4yr degree. Everyone should mentor others to keep things going. Lastly, as techs we need to get out of the 'I am not going to train someone to push me out' mentality. We can all learn from each other. That is why so many new techs can only operate an instrument and not understand the theory. It makes the whole field look bad. Unregistered HT's were provided the opportunity to take the test. Some could not afford it, others did not see the need. Now some are locked into a job and cannot leave because of it. Hospitals are requiring certification for employment that is good. It is a step towards improving the field. I will never say that registered techs are better than unregistered ones, but I will say that those letters behind your name can get you a little further ahead nowadays. My mama taught me that. Sincerely, Toysha N. Mayer, MBA, HT(ASCP) tnmayer@mdanderson.org Instructor/Education Coordinator Program in Histotechnology School of Health Professions MD Anderson Cancer Center 713-563.3481 This very long thread deals with a very complicated and ages long issue so I would like to add my opinion. The fundamental issue is that the pathologists do not respect the histotechs because for them the only thing that matters is that the sections are good, well stained and finished on time. That is all! If they can get some well trained chimpanzees doing these tasks they would be OK with that and they do not give a dam about how much we make or what we know as long as the sections are goo, well stained and on time. Sometimes they decide to "do something" is a histotech completely sick and tired of being disrespected threatens to leave to other lab. The other factor against the histotechs are the managers that prefer to pay the least amount possible and see a histotech with higher education as a potential "money pit" for their budget because they will have to pay them more. Additionally some histotech with higher education are not the best from the quality results point of view and perhaps those with more experience and quality of work are those old histotechs with 20 or more years of experience that usually have been grandfathered and some not even graduated from high school. When I started in this trade (1952) I remember that I was in pre-medical year and learning how to do the basics (embed, section, stain) with the hope of being contracted at the "wonderful" salary of $30/month but that was not to be because the professor head of the department gave the position to a cousin of him and I was supposed to train her, something that did not occur because I left and he had to start all over again. Hiring a janitor or a?cleaning lady to do histology work was not an infrequent occurrence in the mid 1950's and even 30 years later. Why? Again because what the pathologists wanted out of the histotech i.e. good sections the cheapest the better. How this can be solved? It has to start with the pathologists and the administrators, and also with the histotech. We need to demand respect and I coincide that the NSH has done little in that respect. The issue is not only to study and become more knowledgeable in our trade, that is of paramount importance to do a better job, to be able to understand the procedures and be able to trouble shoot but unfortunately many histotechs see this trade as "a decent way or earning a salary" and that is all. Two final caveats: not all pathologists?are born equal, neither all?administrators. Ren? J.? From njmcvay234 <@t> gmail.com Wed Sep 11 17:38:09 2013 From: njmcvay234 <@t> gmail.com (nancy mcvay) Date: Wed Sep 11 17:38:12 2013 Subject: [Histonet] Help Message-ID: Get me off your mailing list. I have tried and keep getting these things. Over and over you repeat the same questions and answers , over and over, over and over, over and over, over and over. Do you get the message?"???? Do you get the message????? Do you get the message????? Get me off your mailing list Get me off your mailing list Today I received four different email tirades from you. Please get me off your mailing list. I am to the point of reporting it as spam. njmcvay234@gmail.com From VEBain <@t> mdanderson.org Wed Sep 11 17:56:51 2013 From: VEBain <@t> mdanderson.org (Bain,Virginia) Date: Wed Sep 11 17:57:55 2013 Subject: [Histonet] Help In-Reply-To: Message-ID: Googling 'unsubscribe histonet' points me to this link: http://lists.utsouthwestern.edu/mailman/listinfo/histonet If you scroll to the bottom of the page you will find a box where you can type your e-mail address to unsubscribe or edit options. Have you tried that? Cheers. -- Virginia Bain Postdoctoral Fellow Richie Lab 512-237-6443 On 9/11/13 5:38 PM, "nancy mcvay" wrote: >Get me off your mailing list. >I have tried and keep getting these things. >Over and over you repeat the same questions and answers , over and over, >over and over, over and over, over and over. > >Do you get the message?"???? >Do you get the message????? >Do you get the message????? >Get me off your mailing list >Get me off your mailing list > > >Today I received four different email tirades from you. > > >Please get me off your mailing list. > >I am to the point of reporting it as spam. > >njmcvay234@gmail.com >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MYang <@t> emc.org Wed Sep 11 18:12:33 2013 From: MYang <@t> emc.org (Yang, Mari) Date: Wed Sep 11 18:12:38 2013 Subject: [Histonet] Cytotech Per Diem Position in Rancho Mirage, CA Message-ID: <5F4186AA4871E74CB729E1B43BAAF731429E8B@NT269.info.sys> I just wanted to share that I have a per diem Cytotech position available. If interested, please visit www.emc.org. Eisenhower Medical Center located in Rancho Mirage, CA is a fantastic place to work. The beautiful winters and lush community atmosphere is most desirable. The Cytology team is a close-knit team with wonderful peer relations. Most important, the non-gyn cases are extremely interesting! Thank you all for your interest and I hope to receive many applications. Warm regards, Mari Mari Yang, MHA, CT(ASCP)CMHTLCM Cytology Supervisor Eisenhower Medical Center Tel: 760.773.2009 P Save a tree, please don't print this e-mail unless you really need to. Confidentiality Note: The preceding e-mail message (including any attachments) contains information that may be confidential, protected by applicable legal privileges, or constitute non-public information. It is intended to be conveyed only to the designated recipient(s). If you are not an intended recipient of this message, please notify the sender by replying to this message and then delete it from your system. Use, dissemination, distribution or reproduction of this message by unintended recipients is not authorized and may be unlawful. From joelleweaver <@t> hotmail.com Wed Sep 11 18:25:04 2013 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Wed Sep 11 18:25:08 2013 Subject: [Histonet] Equipment selection In-Reply-To: <4F36EC93A5737D4F8A2974E8FB8E260615F5698BE8@PHX-MSG-007-N2.chw.edu> References: <4F36EC93A5737D4F8A2974E8FB8E260615F5698BE8@PHX-MSG-007-N2.chw.edu> Message-ID: I think all of these you listed are solid instruments. I am building a lab now, so recently had to pick instruments myself and would have liked to have had these options! From your list I would personally pick the Prisma and Bond Max , just on my personal experiences. I have a Leica ST5020 now, and while it is a good stainer, I hate the clips with the older model I have. Consider if you will use glass or tape coverslipping, the multi-stainer glass coverslipper that goes with it is high maintenance, though it works fine when it works. I personally think the Prisma is great, has super throughput, nice staining. But used to only have tape coverslipping, but may have glass coverslips availible now-if that is a requirement of your pathologists. So check into that. The need for a very open IHC platform ( considering if you do research v. clinical, use mostly concentrated v. prediluted antibodies, the training level of the staff, and your throughput needs as considerations; for example) may help in your choice. The DAKO is awesome , but check whether it still needs more off line pretreatment steps. This adds to the tech time, which might matter considering your staffing and volumes. The staining is fantastic if you can work with it and have staffing and your volumes are managable. The Bond does all steps in automated runs, if you wish; I personally consider it middle ground in terms of "open-ness" somewhere between Ventana and Dako autostainer of years ago. The Bond is a super instrument in my opinion for the right lab situation. I use one now, and it works for me ( I do RTU and a fair number of concentrates) and a good number if ISH probes, and do all the tech work myself. I value the fast TAT with consistent staining-it does the job I need it to do. Good luck Joelle Weaver MAOM, HTL (ASCP) QIHC > From: Haley.Huggins@DignityHealth.org > To: histonet@lists.utsouthwestern.edu > Date: Wed, 11 Sep 2013 13:46:00 -0700 > Subject: [Histonet] Equipment selection > > Hi all, > > I am trying to decide on what equipment to purchase. I have picked the processor, microtomes, embedding centers and a few other pieces, but now I still need to decide on the H&E Stainer and the immunostainer. My options I have in front of me are for the H&E stainer are Sakura Prisma stainer/film coverslipper and the Leica ST5020-CV5030 stainer/glass coverslipper. For the Immunostainer, I am looking at the Dako Autostainer and the Leica Bond Max. Any advice on any of these instruments would be greatly appreciated. I am trying to finalize my decision pretty quickly. Thank you in advance for any input. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Wed Sep 11 18:43:48 2013 From: jqb7 <@t> cdc.gov (Sanders, Jeanine (CDC/OID/NCEZID)) Date: Wed Sep 11 18:44:20 2013 Subject: [Histonet] Unregistered HT In-Reply-To: References: <41D3A1AF6FEF0643BDC89E0516A6EA32D31581E1@Mail2Node2.ad.uams.edu> <41D3A1AF6FEF0643BDC89E0516A6EA32D3158371@Mail2Node2.ad.uams.edu> <1378920615.79308.YahooMailNeo@web163104.mail.bf1.yahoo.com> <5A2BD13465E061429D6455C8D6B40E39165E330DA5@IBMB7Exchange.digestivespecialists.com> <41D3A1AF6FEF0643BDC89E0516A6EA32D31586ED@Mail2Node2.ad.uams.edu> <055f378f3add412a8a95c34a241bb988@BY2PR07MB106.namprd07.prod.outlook.com> Message-ID: Do it! For all of us! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Wednesday, September 11, 2013 3:24 PM Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Unregistered HT Seriously, I can go home?! I'm going to tell my boss that Sarah Dysart said I could go home. When she asks who that is, I'll just look at her and repeat your name. Slowly. And walk away. Emily "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." -Chuck Palahniuk, "Haunted" On Wed, Sep 11, 2013 at 3:07 PM, Sarah Dysart wrote: > > I hope everyone has a great rest of your day, and some people I think > need to go home and have a drink =) > > Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna > Therapeutics > 2150 Woodward Street > Suite 100 > Austin, Texas 78744 > (512)901-0900 ext. 6912 > > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jaylundgren <@t> gmail.com Wed Sep 11 18:51:57 2013 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Wed Sep 11 18:52:03 2013 Subject: [Histonet] Help In-Reply-To: References: Message-ID: lol On Wed, Sep 11, 2013 at 5:56 PM, Bain,Virginia wrote: > Googling 'unsubscribe histonet' points me to this link: > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > If you scroll to the bottom of the page you will find a box where you can > type your e-mail address to unsubscribe or edit options. Have you tried > that? > > Cheers. > > -- > Virginia Bain > Postdoctoral Fellow > Richie Lab > 512-237-6443 > > > > > > On 9/11/13 5:38 PM, "nancy mcvay" wrote: > > >Get me off your mailing list. > >I have tried and keep getting these things. > >Over and over you repeat the same questions and answers , over and over, > >over and over, over and over, over and over. > > > >Do you get the message?"???? > >Do you get the message????? > >Do you get the message????? > >Get me off your mailing list > >Get me off your mailing list > > > > > >Today I received four different email tirades from you. > > > > > >Please get me off your mailing list. > > > >I am to the point of reporting it as spam. > > > >njmcvay234@gmail.com > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From lowenjeff <@t> hotmail.com Wed Sep 11 19:21:52 2013 From: lowenjeff <@t> hotmail.com (jeff lowen) Date: Wed Sep 11 19:21:57 2013 Subject: [Histonet] Unregistered HT In-Reply-To: References: , , , <41D3A1AF6FEF0643BDC89E0516A6EA32D31581E1@Mail2Node2.ad.uams.edu>, , <41D3A1AF6FEF0643BDC89E0516A6EA32D3158371@Mail2Node2.ad.uams.edu>, , , , , <1378920615.79308.YahooMailNeo@web163104.mail.bf1.yahoo.com>, <5A2BD13465E061429D6455C8D6B40E39165E330DA5@IBMB7Exchange.digestivespecialists.com>, , <41D3A1AF6FEF0643BDC89E0516A6EA32D31586ED@Mail2Node2.ad.uams.edu>, <055f378f3add412a8a95c34a241bb988@BY2PR07MB106.namprd07.prod.outlook.com>, , Message-ID: We may not be considered "laboratory professionals", but we might just have more fun than they do ? From: jqb7@cdc.gov To: talulahgosh@gmail.com Date: Wed, 11 Sep 2013 23:43:48 +0000 Subject: RE: [Histonet] Unregistered HT CC: histonet@lists.utsouthwestern.edu Do it! For all of us! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Wednesday, September 11, 2013 3:24 PM Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Unregistered HT Seriously, I can go home?! I'm going to tell my boss that Sarah Dysart said I could go home. When she asks who that is, I'll just look at her and repeat your name. Slowly. And walk away. Emily "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." -Chuck Palahniuk, "Haunted" On Wed, Sep 11, 2013 at 3:07 PM, Sarah Dysart wrote: > > I hope everyone has a great rest of your day, and some people I think > need to go home and have a drink =) > > Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna > Therapeutics > 2150 Woodward Street > Suite 100 > Austin, Texas 78744 > (512)901-0900 ext. 6912 > > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From adesupo2002 <@t> hotmail.com Wed Sep 11 20:02:51 2013 From: adesupo2002 <@t> hotmail.com (ADESUPO ADESUYI) Date: Wed Sep 11 20:02:56 2013 Subject: [Histonet] Histology Lab Plan Message-ID: Hi, Please I will appreciate it if you guys could share your histology lab plan with me especially the lean type. We are in the process of building a new lean histology lab and all new suggestions will be appreciated. Thanks, Adesupo. From Susan.Walzer <@t> HCAHealthcare.com Thu Sep 12 02:16:23 2013 From: Susan.Walzer <@t> HCAHealthcare.com (Susan.Walzer@HCAHealthcare.com) Date: Thu Sep 12 02:16:30 2013 Subject: [Histonet] Unregistered HT In-Reply-To: References: <41D3A1AF6FEF0643BDC89E0516A6EA32D31581E1@Mail2Node2.ad.uams.edu> <41D3A1AF6FEF0643BDC89E0516A6EA32D3158371@Mail2Node2.ad.uams.edu> Message-ID: <4BF03F5404EBDE409AF9232DA74B9DED2FA66183BA@FWDCWPMSGCMS09.hca.corpad.net> I had a pathologist who said we were all " laboratory scientists", now if the pay reflected that.... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Wednesday, September 11, 2013 9:58 AM Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Unregistered HT Do you get paid more with that title? Just curious, I'm not sure what "laboratory professional" means, as I'm not in that kind of lab. Or is it a respect thing? Emily "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." -Chuck Palahniuk, "Haunted" On Tue, Sep 10, 2013 at 7:32 PM, Jennifer MacDonald wrote: > As long as we do not need certification, licensure and minium > education requirements we will not be recognized as Laboratory Professionals. > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BSullivan <@t> virtua.org Thu Sep 12 06:39:29 2013 From: BSullivan <@t> virtua.org (Sullivan, Beatrice) Date: Thu Sep 12 06:39:41 2013 Subject: [Histonet] RE: Unregistered HT In-Reply-To: <47E9B2C01DDDD94881EACD2DC44EBC8802B24F2C@D1PWPEXMBX05.mdanderson.edu> References: <47E9B2C01DDDD94881EACD2DC44EBC8802B24F2C@D1PWPEXMBX05.mdanderson.edu> Message-ID: <6932520047F7EE46B512E9801344F160380C92F2@ExchangeMB-1.Virtua.org> Amen to that ! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mayer,Toysha N Sent: Wednesday, September 11, 2013 5:04 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE:Unregistered HT I actually agree with Rene on some points. In the past, and in some current labs, that mentality of just get the work done prevails. It happens more in small labs than in larger ones now. However the tide is starting to change. While it may be years before the old regime mentality retires out (we all know that pathologists don't really retire they hang around forever, no offense Samurai pathologist), management is changing. We must demonstrate to ourselves that we matter. Too many places still allow the docs to control the lab and it has hindered us. It has to do with the separation of medicine and business. Not all docs think monkeys can do our job, I know some that understand our value. I don't know what category to place myself in, an old timer or a young buck, I have 20 years in and have paid my dues. I was disrespected, overlooked and scraped floors, but I stayed around. Sometimes I take offense at those who discredit the OJT route, I did it. But I also had a BS degree. I take offense at those who look down on me because I do have a degree, and passed my test. Those were my choices, and I wanted to better position myself for promotion. Some really good techs have no degree, or certification and I would consult with them in a heartbeat. I care about what I do, it matters, so I take pride in my sections and stains. To get our respect we should support our schools (not just because I teach) because they are the ones who can demonstrate the proper skills to students. To do this realize that schools need students to stay open, so send some their way. The faculty cannot teach without them. Be willing to serve as a clinical site to teach what you know, especially if you think they are doing it wrong. Remember that the faculty are under pressure to graduate students so make sure that you support them, don't get mad when they take in more students. We all want to eat. Suggest to your HT's that they go back to get a BS to move up. Facilities are beginning to require supervisors and managers to have that 4yr degree. Everyone should mentor others to keep things going. Lastly, as techs we need to get out of the 'I am not going to train someone to push me out' mentality. We can all learn from each other. That is why so many new techs can only operate an instrument and not understand the theory. It makes the whole field look bad. Unregistered HT's were provided the opportunity to take the test. Some could not afford it, others did not see the need. Now some are locked into a job and cannot leave because of it. Hospitals are requiring certification for employment that is good. It is a step towards improving the field. I will never say that registered techs are better than unregistered ones, but I will say that those letters behind your name can get you a little further ahead nowadays. My mama taught me that. Sincerely, Toysha N. Mayer, MBA, HT(ASCP) tnmayer@mdanderson.org Instructor/Education Coordinator Program in Histotechnology School of Health Professions MD Anderson Cancer Center 713-563.3481 This very long thread deals with a very complicated and ages long issue so I would like to add my opinion. The fundamental issue is that the pathologists do not respect the histotechs because for them the only thing that matters is that the sections are good, well stained and finished on time. That is all! If they can get some well trained chimpanzees doing these tasks they would be OK with that and they do not give a dam about how much we make or what we know as long as the sections are goo, well stained and on time. Sometimes they decide to "do something" is a histotech completely sick and tired of being disrespected threatens to leave to other lab. The other factor against the histotechs are the managers that prefer to pay the least amount possible and see a histotech with higher education as a potential "money pit" for their budget because they will have to pay them more. Additionally some histotech with higher education are not the best from the quality results point of view and perhaps those with more experience and quality of work are those old histotechs with 20 or more years of experience that usually have been grandfathered and some not even graduated from high school. When I started in this trade (1952) I remember that I was in pre-medical year and learning how to do the basics (embed, section, stain) with the hope of being contracted at the "wonderful" salary of $30/month but that was not to be because the professor head of the department gave the position to a cousin of him and I was supposed to train her, something that did not occur because I left and he had to start all over again. Hiring a janitor or a?cleaning lady to do histology work was not an infrequent occurrence in the mid 1950's and even 30 years later. Why? Again because what the pathologists wanted out of the histotech i.e. good sections the cheapest the better. How this can be solved? It has to start with the pathologists and the administrators, and also with the histotech. We need to demand respect and I coincide that the NSH has done little in that respect. The issue is not only to study and become more knowledgeable in our trade, that is of paramount importance to do a better job, to be able to understand the procedures and be able to trouble shoot but unfortunately many histotechs see this trade as "a decent way or earning a salary" and that is all. Two final caveats: not all pathologists?are born equal, neither all?administrators. Ren? J.? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message, and any included attachments, are from Virtua Health or its related affiliates and is intended only for the addressee(s). The information contained herein is privileged, proprietary or may include confidential information and/or protected patient health information. Any unauthorized review, forwarding, printing, copying, distributing, or otherwise disseminating or taking any action based on such information is strictly prohibited. If you have received this message in error, or have reason to believe you are not authorized to receive it, please delete this message promptly and notify the sender by e-mail with a copy to ISSECURITY@virtua.org. Thank you From HornHV <@t> archildrens.org Thu Sep 12 07:12:20 2013 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Thu Sep 12 07:12:29 2013 Subject: [Histonet] RE: Equipment selection In-Reply-To: <4F36EC93A5737D4F8A2974E8FB8E260615F5698BE8@PHX-MSG-007-N2.chw.edu> References: <4F36EC93A5737D4F8A2974E8FB8E260615F5698BE8@PHX-MSG-007-N2.chw.edu> Message-ID: <25A4DE08332B19499904459F00AAACB719C704FD7C@EVS1.archildrens.org> We have the Leica stainer and coverslipper and we like it. The only drawback to our stainer is it only has 1 oven. But the stainer and the coverslipper have been reliable and are easy to operate. We have the Leica Bond for immunos and we love it! It's user friendly, very reliable and we have many options for antibody vendors. Leica is a great company for support. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Huggins, Haley - MRMC Sent: Wednesday, September 11, 2013 3:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Equipment selection Hi all, I am trying to decide on what equipment to purchase. I have picked the processor, microtomes, embedding centers and a few other pieces, but now I still need to decide on the H&E Stainer and the immunostainer. My options I have in front of me are for the H&E stainer are Sakura Prisma stainer/film coverslipper and the Leica ST5020-CV5030 stainer/glass coverslipper. For the Immunostainer, I am looking at the Dako Autostainer and the Leica Bond Max. Any advice on any of these instruments would be greatly appreciated. I am trying to finalize my decision pretty quickly. Thank you in advance for any input. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From rjbuesa <@t> yahoo.com Thu Sep 12 07:32:26 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Sep 12 07:32:37 2013 Subject: [Histonet] Equipment selection In-Reply-To: <4F36EC93A5737D4F8A2974E8FB8E260615F5698BE8@PHX-MSG-007-N2.chw.edu> References: <4F36EC93A5737D4F8A2974E8FB8E260615F5698BE8@PHX-MSG-007-N2.chw.edu> Message-ID: <1378989146.57715.YahooMailNeo@web163104.mail.bf1.yahoo.com> I used the Sakura and the Dako, but it seems that the Leica Bond Max is getting popularity. Why don't you? go to a lab where they are in use and take a look? Ren? J. ________________________________ From: "Huggins, Haley - MRMC" To: "histonet@lists.utsouthwestern.edu" Sent: Wednesday, September 11, 2013 4:46 PM Subject: [Histonet] Equipment selection Hi all, I am trying to decide on what equipment to purchase. I have picked the processor, microtomes, embedding centers and a few other pieces, but now I still need to decide on the H&E Stainer and the immunostainer. My options I have in front of me are for the H&E stainer are Sakura Prisma stainer/film coverslipper and the Leica ST5020-CV5030 stainer/glass coverslipper. For the Immunostainer, I am looking at the Dako Autostainer and the Leica Bond Max. Any advice on any of these instruments would be greatly appreciated. I am trying to finalize my decision pretty quickly. Thank you in advance for any input. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From talulahgosh <@t> gmail.com Thu Sep 12 07:51:45 2013 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Thu Sep 12 07:51:50 2013 Subject: [Histonet] Help In-Reply-To: References: Message-ID: FACEPALM.JPG this is not even worth an actual jpg. sigh. "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." -Chuck Palahniuk, "Haunted" On Wed, Sep 11, 2013 at 7:51 PM, Jay Lundgren wrote: > lol > > > On Wed, Sep 11, 2013 at 5:56 PM, Bain,Virginia >wrote: > > > Googling 'unsubscribe histonet' points me to this link: > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > If you scroll to the bottom of the page you will find a box where you can > > type your e-mail address to unsubscribe or edit options. Have you tried > > that? > > > > Cheers. > > > > -- > > Virginia Bain > > Postdoctoral Fellow > > Richie Lab > > 512-237-6443 > > > > > > > > > > > > On 9/11/13 5:38 PM, "nancy mcvay" wrote: > > > > >Get me off your mailing list. > > >I have tried and keep getting these things. > > >Over and over you repeat the same questions and answers , over and > over, > > >over and over, over and over, over and over. > > > > > >Do you get the message?"???? > > >Do you get the message????? > > >Do you get the message????? > > >Get me off your mailing list > > >Get me off your mailing list > > > > > > > > >Today I received four different email tirades from you. > > > > > > > > >Please get me off your mailing list. > > > > > >I am to the point of reporting it as spam. > > > > > >njmcvay234@gmail.com > > >_______________________________________________ > > >Histonet mailing list > > >Histonet@lists.utsouthwestern.edu > > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From mward <@t> wakehealth.edu Thu Sep 12 08:06:55 2013 From: mward <@t> wakehealth.edu (Martha Ward-Pathology) Date: Thu Sep 12 08:07:01 2013 Subject: [Histonet] Minimum sample size for breast markers In-Reply-To: <522F1138020000BC0005ECF4@gwia-out.peigov> References: <522F1138020000BC0005ECF4@gwia-out.peigov> Message-ID: Is there a minimum number of cells that should be present when testing for ER, PR and Her2? I have looked on the CAP website but cannot see anything about this. We are constantly getting these small biopsies or FNAs for testing and I sometimes wonder what the lowest threshold is for testing...20 tumor cells, 50 tumor cells???? Any information would be appreciated. Thanks in advance for your help. ? Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard ?\? Winston-Salem, NC 27157 p 336.716.2109 ?\? f 336.716.5890 ? mward@wakehealth.edu ? ? ? From talulahgosh <@t> gmail.com Thu Sep 12 08:11:19 2013 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Thu Sep 12 08:11:27 2013 Subject: [Histonet] Unregistered HT In-Reply-To: <4BF03F5404EBDE409AF9232DA74B9DED2FA66183BA@FWDCWPMSGCMS09.hca.corpad.net> References: <41D3A1AF6FEF0643BDC89E0516A6EA32D31581E1@Mail2Node2.ad.uams.edu> <41D3A1AF6FEF0643BDC89E0516A6EA32D3158371@Mail2Node2.ad.uams.edu> <4BF03F5404EBDE409AF9232DA74B9DED2FA66183BA@FWDCWPMSGCMS09.hca.corpad.net> Message-ID: Ha, surely you kid! I didn't go into science for the money, but only because I love it. Even though I get paid horribly (seriously, I qualify for Medicaid when the government includes my husband's income!), I love being in a lab. This is with a BS in Microbiology. I try not to think about the money part of it. Emily "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." -Chuck Palahniuk, "Haunted" On Thu, Sep 12, 2013 at 3:16 AM, wrote: > I had a pathologist who said we were all " laboratory scientists", now if > the pay reflected that.... > > > From Lisa.White3 <@t> va.gov Thu Sep 12 08:16:25 2013 From: Lisa.White3 <@t> va.gov (White, Lisa M.) Date: Thu Sep 12 08:17:50 2013 Subject: [Histonet] ThinPrep Message-ID: <2B2ECF33934F5D4996D8BE03EFDF397607D8A092@VHAV09MSGA3.v09.med.va.gov> Does anyone know a CAP regulation for validation of ThinPrep? Lisa White HT(ASCP) Supervisory HT James H. Quillen VAMC Corner of Veterans Way and Lamont VAMC Warehouse BLDG. 205 PO Box 4000 PLMS 113 Mountain Home, TN 37684 423-979-3567 423-979-3401 fax From rsrichmond <@t> gmail.com Thu Sep 12 08:22:06 2013 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Thu Sep 12 08:22:12 2013 Subject: [Histonet] Re: Unregistered HT Message-ID: Since somebody mentioned the Samurai Pathologist (who is now 74 years old and in his 50th year in pathology) - I agree with most of what's been said here and I won't repeat it. >From the pathologist's viewpoint - remember that most pathologists are now on salary (or soon will be) and don't have a dog in the fight about doing the job as cheaply as possible. I think that a very large part of the problem is that most pathologists haven't a clue as to what goes on in the histology lab (that's why we cram cassettes full of fatty breast tissue), and that pathologists need to acquire this knowledge in residency, to the degree that they can teach and trouble-shoot or work with senior technologists who can. It's particularly important that pathologists learn to embed. Edwards Deming was an economist who grew up in "operations research" during World War 2. After the War he tried to get the automotive industry to adopt his methods. The executives laughed at him (and still do in the business schools, I think), so he took his ideas to Japan, where they built the Japanese automative industry. Deming's major idea (if I understand him correctly) was that workers need constant specific feedback about what they're doing. I think that the establishment of effective feedback from pathologist to histotechnologist is the first step in solving the problem we've been talking about. And I think that means a pathologist sitting down with a histotechnologist and reviewing some of the day's slides every blessed day. Bob Richmond Samurai Pathologist Maryville TN From rjbuesa <@t> yahoo.com Thu Sep 12 08:22:34 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Sep 12 08:22:38 2013 Subject: [Histonet] Minimum sample size for breast markers In-Reply-To: References: <522F1138020000BC0005ECF4@gwia-out.peigov> Message-ID: <1378992154.20733.YahooMailNeo@web163104.mail.bf1.yahoo.com> I do not think this is the adequate forum to ask this question because you may get a wrong answer that could?determine your decision. Your pathologist is the one who should?decide about this issue. Ren? J. ________________________________ From: Martha Ward-Pathology To: "histonet@lists.utsouthwestern.edu" Sent: Thursday, September 12, 2013 9:06 AM Subject: [Histonet] Minimum sample size for breast markers Is there a minimum number of cells that should be present when testing for ER, PR and Her2? I have looked on the CAP website but cannot see anything about this.? We are constantly getting these small biopsies or FNAs for testing and I sometimes wonder what the lowest threshold is for testing...20 tumor cells, 50 tumor cells????? Any information would be appreciated.? Thanks in advance for your help. ? Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard ?\? Winston-Salem, NC 27157 p 336.716.2109 ?\? f 336.716.5890 ? mward@wakehealth.edu ? ? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lowenjeff <@t> hotmail.com Thu Sep 12 08:31:05 2013 From: lowenjeff <@t> hotmail.com (jeff lowen) Date: Thu Sep 12 08:31:09 2013 Subject: [Histonet] Re: Unregistered HT In-Reply-To: References: Message-ID: This also brings up the "made in China" situation where it can be crap or perfectly good, depending on raw materials but more important, how closely and by who are the production lines being monitored for QA and QC.They can make stuff as good as anybody, maybe? > Date: Thu, 12 Sep 2013 09:22:06 -0400 > From: rsrichmond@gmail.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: Unregistered HT > > Since somebody mentioned the Samurai Pathologist (who is now 74 years old > and in his 50th year in pathology) - > > I agree with most of what's been said here and I won't repeat it. > > >From the pathologist's viewpoint - remember that most pathologists are now > on salary (or soon will be) and don't have a dog in the fight about doing > the job as cheaply as possible. > > I think that a very large part of the problem is that most pathologists > haven't a clue as to what goes on in the histology lab (that's why we cram > cassettes full of fatty breast tissue), and that pathologists need to > acquire this knowledge in residency, to the degree that they can teach and > trouble-shoot or work with senior technologists who can. It's particularly > important that pathologists learn to embed. > > Edwards Deming was an economist who grew up in "operations research" during > World War 2. After the War he tried to get the automotive industry to adopt > his methods. The executives laughed at him (and still do in the business > schools, I think), so he took his ideas to Japan, where they built the > Japanese automative industry. Deming's major idea (if I understand him > correctly) was that workers need constant specific feedback about what > they're doing. > > I think that the establishment of effective feedback from pathologist to > histotechnologist is the first step in solving the problem we've been > talking about. And I think that means a pathologist sitting down with a > histotechnologist and reviewing some of the day's slides every blessed day. > > Bob Richmond > Samurai Pathologist > Maryville TN > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PAMarcum <@t> uams.edu Thu Sep 12 08:41:44 2013 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Thu Sep 12 08:41:50 2013 Subject: [Histonet] Re: Unregistered HT In-Reply-To: References: Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA32D31589F6@Mail2Node2.ad.uams.edu> Thank you Bob! I remember when pathology residents had to spend some time in histology learning at least enough to know we were a team not enemies. Residents now barely get to see the Histology Lab so expecting them to see us as anything other than labor is a part of the issue. Their world is changing and I am not sure how it will play out for AP as whole. Deming was very sure as you stated that worker feedback and more importantly input into the process of every area of manufacturing was the key to having a both a creative and happy workforce. Deming wanted to encourage creativity for the workers so they were part of the whole process. We are rarely asked for input in any area of the work arena due to the tiered system that has developed in all areas of employment today. It is usually the squeaky wheel that get the grease or in this improvements in any area of the workplace and life. Pam Marcum -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Thursday, September 12, 2013 8:22 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Unregistered HT Since somebody mentioned the Samurai Pathologist (who is now 74 years old and in his 50th year in pathology) - I agree with most of what's been said here and I won't repeat it. >From the pathologist's viewpoint - remember that most pathologists are >now on salary (or soon will be) and don't have a dog in the fight about doing the job as cheaply as possible. I think that a very large part of the problem is that most pathologists haven't a clue as to what goes on in the histology lab (that's why we cram cassettes full of fatty breast tissue), and that pathologists need to acquire this knowledge in residency, to the degree that they can teach and trouble-shoot or work with senior technologists who can. It's particularly important that pathologists learn to embed. Edwards Deming was an economist who grew up in "operations research" during World War 2. After the War he tried to get the automotive industry to adopt his methods. The executives laughed at him (and still do in the business schools, I think), so he took his ideas to Japan, where they built the Japanese automative industry. Deming's major idea (if I understand him correctly) was that workers need constant specific feedback about what they're doing. I think that the establishment of effective feedback from pathologist to histotechnologist is the first step in solving the problem we've been talking about. And I think that means a pathologist sitting down with a histotechnologist and reviewing some of the day's slides every blessed day. Bob Richmond Samurai Pathologist Maryville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From Richard.Cartun <@t> hhchealth.org Thu Sep 12 08:54:09 2013 From: Richard.Cartun <@t> hhchealth.org (Cartun, Richard) Date: Thu Sep 12 08:54:14 2013 Subject: [Histonet] RE: Minimum sample size for breast markers In-Reply-To: References: <522F1138020000BC0005ECF4@gwia-out.peigov>, Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E01882D59@HHCEXCHMB05.hhcsystem.org> There is no minimum as far as I'm concerned. I have tested as few as "5" malignant cells; however, I always put a statement in the report indicating "limited tumor present for evaluation". It is then up to the clinician to determine whether to start (or continue) therapy or to get another sample for testing (if possible). Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax richard.cartun@hhchealth.org ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Martha Ward-Pathology [mward@wakehealth.edu] Sent: Thursday, September 12, 2013 9:06 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Minimum sample size for breast markers Is there a minimum number of cells that should be present when testing for ER, PR and Her2? I have looked on the CAP website but cannot see anything about this. We are constantly getting these small biopsies or FNAs for testing and I sometimes wonder what the lowest threshold is for testing...20 tumor cells, 50 tumor cells???? Any information would be appreciated. Thanks in advance for your help. Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mward@wakehealth.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From joelleweaver <@t> hotmail.com Thu Sep 12 08:58:15 2013 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Thu Sep 12 08:58:19 2013 Subject: [Histonet] Re: Unregistered HT In-Reply-To: References: Message-ID: This is one of the best posts I have read on this subject, along with Tim Morken's positive one from yesterday. Feedback ! Collaboration ! Learning! That is what we need to bring all us up. It comes down to you and what you want to get out of it, but glad to hear the Pathologists view. Managers have to be on board too. It takes everyone to generate change. And maybe people didn't understand Deming at the time, but they had definitely come to appreciate Deming by the time I studied business, and my business professors were not laughing at his ideas. Food for thought. Joelle Weaver MAOM, HTL (ASCP) QIHC > Date: Thu, 12 Sep 2013 09:22:06 -0400 > From: rsrichmond@gmail.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: Unregistered HT > > Since somebody mentioned the Samurai Pathologist (who is now 74 years old > and in his 50th year in pathology) - > > I agree with most of what's been said here and I won't repeat it. > > >From the pathologist's viewpoint - remember that most pathologists are now > on salary (or soon will be) and don't have a dog in the fight about doing > the job as cheaply as possible. > > I think that a very large part of the problem is that most pathologists > haven't a clue as to what goes on in the histology lab (that's why we cram > cassettes full of fatty breast tissue), and that pathologists need to > acquire this knowledge in residency, to the degree that they can teach and > trouble-shoot or work with senior technologists who can. It's particularly > important that pathologists learn to embed. > > Edwards Deming was an economist who grew up in "operations research" during > World War 2. After the War he tried to get the automotive industry to adopt > his methods. The executives laughed at him (and still do in the business > schools, I think), so he took his ideas to Japan, where they built the > Japanese automative industry. Deming's major idea (if I understand him > correctly) was that workers need constant specific feedback about what > they're doing. > > I think that the establishment of effective feedback from pathologist to > histotechnologist is the first step in solving the problem we've been > talking about. And I think that means a pathologist sitting down with a > histotechnologist and reviewing some of the day's slides every blessed day. > > Bob Richmond > Samurai Pathologist > Maryville TN > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TGoins <@t> mt.gov Thu Sep 12 09:02:37 2013 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Thu Sep 12 09:02:49 2013 Subject: [Histonet] Help In-Reply-To: References: Message-ID: Over and over and over and over? You must have missed the unsubscribe instructions that have been discussed over and over and over and over . . . . . . . -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of nancy mcvay Sent: Wednesday, September 11, 2013 4:38 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Help Get me off your mailing list. I have tried and keep getting these things. Over and over you repeat the same questions and answers , over and over, over and over, over and over, over and over. Do you get the message?"???? Do you get the message????? Do you get the message????? Get me off your mailing list Get me off your mailing list Today I received four different email tirades from you. Please get me off your mailing list. I am to the point of reporting it as spam. njmcvay234@gmail.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From corey <@t> rankinbiomed.com Thu Sep 12 09:15:47 2013 From: corey <@t> rankinbiomed.com (Corey Hubbard) Date: Thu Sep 12 09:15:50 2013 Subject: [Histonet] (no subject) Message-ID: corey@rankinbiomedical.com Regards, Corey Hubbard Corey Hubbard Sales Administrator *Welcome to Rankin Biomedical * *Rankin Biomedical Corporation* 14515 Mackey Rd. Holly, MI 48442 (877) 882-3679 Toll Free (248) 625-1070 Fax *~~ Equipping your Lab for Success ~~* ** This is a confidential transmission from Rankin Biomedical Corporation and is intended only for the individual or entity designated above. If you are not the addressee, please note that any disclosure, copying, distribution, or use of or reliance upon the information contained in and transmitted with this e-mail by or to anyone other than the recipient designated above is unauthorized and strictly prohibited. If you have received this transmission in error, please destroy/delete it and notify us immediately at 248 625-4104. ** From JMaslanka <@t> stpetes.org Thu Sep 12 09:22:33 2013 From: JMaslanka <@t> stpetes.org (JMaslanka@stpetes.org) Date: Thu Sep 12 09:23:41 2013 Subject: [Histonet] Unsubscribe Comment Message-ID: Just like the Eagles' "Hotel California" "you can check out (unsubscribe) but you can NEVER leave." An early Happy Friday the Thirteenth. Joe Maslanka BS, CT,HT (ASCP) Anatomical Pathology Technical Supervisor St Peter's Hospital,MT 59601 (P)(406) 447-2406 (F)(406)444-2126 Give thanks for ALL things..... "Kindness is the language the blind can see & the deaf can hear- Mark Twain From mward <@t> wakehealth.edu Thu Sep 12 09:24:10 2013 From: mward <@t> wakehealth.edu (Martha Ward-Pathology) Date: Thu Sep 12 09:24:14 2013 Subject: [Histonet] Minimum sample size for breast markers In-Reply-To: <1378992154.20733.YahooMailNeo@web163104.mail.bf1.yahoo.com> References: <522F1138020000BC0005ECF4@gwia-out.peigov> <1378992154.20733.YahooMailNeo@web163104.mail.bf1.yahoo.com> Message-ID: Thanks Rene. I realize that it is ultimately up to the Pathologist but I was wondering if there are any written guideline/suggestions because I have had pathologists ask me if there is minimum. From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: Thursday, September 12, 2013 9:23 AM To: Martha Ward-Pathology; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Minimum sample size for breast markers I do not think this is the adequate forum to ask this question because you may get a wrong answer that could determine your decision. Your pathologist is the one who should decide about this issue. Ren? J. From: Martha Ward-Pathology > To: "histonet@lists.utsouthwestern.edu" > Sent: Thursday, September 12, 2013 9:06 AM Subject: [Histonet] Minimum sample size for breast markers Is there a minimum number of cells that should be present when testing for ER, PR and Her2? I have looked on the CAP website but cannot see anything about this. We are constantly getting these small biopsies or FNAs for testing and I sometimes wonder what the lowest threshold is for testing...20 tumor cells, 50 tumor cells???? Any information would be appreciated. Thanks in advance for your help. Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mward@wakehealth.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lowenjeff <@t> hotmail.com Thu Sep 12 09:40:44 2013 From: lowenjeff <@t> hotmail.com (jeff lowen) Date: Thu Sep 12 09:40:48 2013 Subject: [Histonet] Unsubscribe Comment In-Reply-To: References: Message-ID: How true, though these days we can "delete", which is pretty much what the Eagles did to more than a few hotel rooms, or so they say? > To: histonet@lists.utsouthwestern.edu > From: JMaslanka@stpetes.org > Date: Thu, 12 Sep 2013 08:22:33 -0600 > Subject: [Histonet] Unsubscribe Comment > > Just like the Eagles' "Hotel California" "you can check out (unsubscribe) > but you can NEVER leave." > An early Happy Friday the Thirteenth. > > > Joe Maslanka BS, CT,HT (ASCP) > Anatomical Pathology Technical Supervisor > St Peter's Hospital,MT 59601 > (P)(406) 447-2406 > (F)(406)444-2126 > > Give thanks for ALL things..... > "Kindness is the language the blind can see & the deaf can hear- Mark > Twain > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Sep 12 09:55:46 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Sep 12 09:55:52 2013 Subject: [Histonet] Minimum sample size for breast markers In-Reply-To: References: <522F1138020000BC0005ECF4@gwia-out.peigov> <1378992154.20733.YahooMailNeo@web163104.mail.bf1.yahoo.com> Message-ID: <1378997746.79101.YahooMailNeo@web163105.mail.bf1.yahoo.com> No, there are no written instructions on the subject. You just have to state how many cells you found and the clinician is supposed to act accordingly. Ren? J. ________________________________ From: Martha Ward-Pathology To: Rene J Buesa ; "histonet@lists.utsouthwestern.edu" Sent: Thursday, September 12, 2013 10:24 AM Subject: RE: [Histonet] Minimum sample size for breast markers Thanks Rene.? I realize that it is ultimately up to the Pathologist but I was wondering if there are any written guideline/suggestions because I have had pathologists ask me if there is minimum. ? From:Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: Thursday, September 12, 2013 9:23 AM To: Martha Ward-Pathology; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Minimum sample size for breast markers ? I do not think this is the adequate forum to ask this question because you may get a wrong answer that could?determine your decision. Your pathologist is the one who should?decide about this issue. Ren?J. ? From:Martha Ward-Pathology To: "histonet@lists.utsouthwestern.edu" Sent: Thursday, September 12, 2013 9:06 AM Subject: [Histonet] Minimum sample size for breast markers Is there a minimum number of cells that should be present when testing for ER, PR and Her2? I have looked on the CAP website but cannot see anything about this.? We are constantly getting these small biopsies or FNAs for testing and I sometimes wonder what the lowest threshold is for testing...20 tumor cells, 50 tumor cells????? Any information would be appreciated.? Thanks in advance for your help. ? Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard ?\? Winston-Salem, NC 27157 p 336.716.2109 ?\? f 336.716.5890 ? mward@wakehealth.edu ? ? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mward <@t> wakehealth.edu Thu Sep 12 10:19:47 2013 From: mward <@t> wakehealth.edu (Martha Ward-Pathology) Date: Thu Sep 12 10:19:53 2013 Subject: [Histonet] Minimum sample size for breast markers In-Reply-To: <1378997746.79101.YahooMailNeo@web163105.mail.bf1.yahoo.com> References: <522F1138020000BC0005ECF4@gwia-out.peigov> <1378992154.20733.YahooMailNeo@web163104.mail.bf1.yahoo.com> <1378997746.79101.YahooMailNeo@web163105.mail.bf1.yahoo.com> Message-ID: Thanks everyone.... From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: Thursday, September 12, 2013 10:56 AM To: Martha Ward-Pathology; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Minimum sample size for breast markers No, there are no written instructions on the subject. You just have to state how many cells you found and the clinician is supposed to act accordingly. Ren? J. From: Martha Ward-Pathology > To: Rene J Buesa >; "histonet@lists.utsouthwestern.edu" > Sent: Thursday, September 12, 2013 10:24 AM Subject: RE: [Histonet] Minimum sample size for breast markers Thanks Rene. I realize that it is ultimately up to the Pathologist but I was wondering if there are any written guideline/suggestions because I have had pathologists ask me if there is minimum. From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: Thursday, September 12, 2013 9:23 AM To: Martha Ward-Pathology; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Minimum sample size for breast markers I do not think this is the adequate forum to ask this question because you may get a wrong answer that could determine your decision. Your pathologist is the one who should decide about this issue. Ren? J. From: Martha Ward-Pathology > To: "histonet@lists.utsouthwestern.edu" > Sent: Thursday, September 12, 2013 9:06 AM Subject: [Histonet] Minimum sample size for breast markers Is there a minimum number of cells that should be present when testing for ER, PR and Her2? I have looked on the CAP website but cannot see anything about this. We are constantly getting these small biopsies or FNAs for testing and I sometimes wonder what the lowest threshold is for testing...20 tumor cells, 50 tumor cells???? Any information would be appreciated. Thanks in advance for your help. Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mward@wakehealth.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From azdudley <@t> hotmail.com Thu Sep 12 10:24:26 2013 From: azdudley <@t> hotmail.com (anita dudley) Date: Thu Sep 12 10:24:29 2013 Subject: [Histonet] ANP.21460 Message-ID: would someone please share how they document the positive tissue controls, we check them all before going out of the lab and marked we have checked. what else are people doing? thanks so much for your input Anita Dudley Providence Hospital Mobile Alabama From HornHV <@t> archildrens.org Thu Sep 12 10:29:46 2013 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Thu Sep 12 10:29:52 2013 Subject: [Histonet] Re: Unregistered HT In-Reply-To: References: Message-ID: <25A4DE08332B19499904459F00AAACB719C704FD84@EVS1.archildrens.org> I agree! You are wise. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Thursday, September 12, 2013 8:22 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Unregistered HT Since somebody mentioned the Samurai Pathologist (who is now 74 years old and in his 50th year in pathology) - I agree with most of what's been said here and I won't repeat it. >From the pathologist's viewpoint - remember that most pathologists are >now on salary (or soon will be) and don't have a dog in the fight about doing the job as cheaply as possible. I think that a very large part of the problem is that most pathologists haven't a clue as to what goes on in the histology lab (that's why we cram cassettes full of fatty breast tissue), and that pathologists need to acquire this knowledge in residency, to the degree that they can teach and trouble-shoot or work with senior technologists who can. It's particularly important that pathologists learn to embed. Edwards Deming was an economist who grew up in "operations research" during World War 2. After the War he tried to get the automotive industry to adopt his methods. The executives laughed at him (and still do in the business schools, I think), so he took his ideas to Japan, where they built the Japanese automative industry. Deming's major idea (if I understand him correctly) was that workers need constant specific feedback about what they're doing. I think that the establishment of effective feedback from pathologist to histotechnologist is the first step in solving the problem we've been talking about. And I think that means a pathologist sitting down with a histotechnologist and reviewing some of the day's slides every blessed day. Bob Richmond Samurai Pathologist Maryville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From Donna.Willis <@t> baylorhealth.edu Thu Sep 12 10:54:28 2013 From: Donna.Willis <@t> baylorhealth.edu (Willis, Donna G.) Date: Thu Sep 12 10:54:39 2013 Subject: [Histonet] ANP.21460 In-Reply-To: References: Message-ID: <2572B4D63B62E64A8078D8BBE34D407833939C@BHDASVEXML2.bhcs.pvt> Your pathologist need to note in the report that both the positive and negative control worked appropriately. Donna Willis, HT/HTL (ASCP) Anatomic Pathology Manager Baylor University Medical Center-Dallas ph. 214-820-2465 office ph. 214-725-6184 mobile donna.willis@baylorhealth.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of anita dudley Sent: Thursday, September 12, 2013 10:24 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] ANP.21460 would someone please share how they document the positive tissue controls, we check them all before going out of the lab and marked we have checked. what else are people doing? thanks so much for your input Anita Dudley Providence Hospital Mobile Alabama _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This e-mail may contain confidential and/or privileged information. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. From Haley.Huggins <@t> DignityHealth.org Thu Sep 12 10:55:39 2013 From: Haley.Huggins <@t> DignityHealth.org (Huggins, Haley - MRMC) Date: Thu Sep 12 10:55:57 2013 Subject: [Histonet] Equipment selection In-Reply-To: <1378989146.57715.YahooMailNeo@web163104.mail.bf1.yahoo.com> References: <4F36EC93A5737D4F8A2974E8FB8E260615F5698BE8@PHX-MSG-007-N2.chw.edu> <1378989146.57715.YahooMailNeo@web163104.mail.bf1.yahoo.com> Message-ID: <4F36EC93A5737D4F8A2974E8FB8E260615F5698C69@PHX-MSG-007-N2.chw.edu> I actually know all the equipment. Well, I know 3 of the pieces really well, the Leica Bond Max is new to me. I did go to another lab to see it as well as the Leica stainer/coverslipper. I was impressed with the Bond Max, not as much with the coverslipper part of the stainer/coverslipper. The big issue I am having is if I want to go with the Bond Max, I technically need to go with the Leica stainer/coverslipper because it is lumped into a big package deal. If I want the Sakura Prisma stainer/film coverslipper, I go with the Dako immunostainer. I am having trouble deciding. It is boiling down to money, which sucks since I might be forced to go with something that I might not be happy with in the future. Haley From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: Thursday, September 12, 2013 5:32 AM To: Huggins, Haley - MRMC; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Equipment selection I used the Sakura and the Dako, but it seems that the Leica Bond Max is getting popularity. Why don't you go to a lab where they are in use and take a look? Ren? J. From: "Huggins, Haley - MRMC" > To: "histonet@lists.utsouthwestern.edu" > Sent: Wednesday, September 11, 2013 4:46 PM Subject: [Histonet] Equipment selection Hi all, I am trying to decide on what equipment to purchase. I have picked the processor, microtomes, embedding centers and a few other pieces, but now I still need to decide on the H&E Stainer and the immunostainer. My options I have in front of me are for the H&E stainer are Sakura Prisma stainer/film coverslipper and the Leica ST5020-CV5030 stainer/glass coverslipper. For the Immunostainer, I am looking at the Dako Autostainer and the Leica Bond Max. Any advice on any of these instruments would be greatly appreciated. I am trying to finalize my decision pretty quickly. Thank you in advance for any input. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Sep 12 11:07:56 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Sep 12 11:08:12 2013 Subject: [Histonet] Equipment selection In-Reply-To: <4F36EC93A5737D4F8A2974E8FB8E260615F5698C69@PHX-MSG-007-N2.chw.edu> References: <4F36EC93A5737D4F8A2974E8FB8E260615F5698BE8@PHX-MSG-007-N2.chw.edu> <1378989146.57715.YahooMailNeo@web163104.mail.bf1.yahoo.com> <4F36EC93A5737D4F8A2974E8FB8E260615F5698C69@PHX-MSG-007-N2.chw.edu> Message-ID: <1379002076.53612.YahooMailNeo@web163105.mail.bf1.yahoo.com> If buying the Bond means that you are forced to "swallow" the Leica coverslipper go with Sakura and Dako. If they are making that "package" is because the Bond sells well?but they are having to "swallow" the coverslipper. Price is important but in the long run it is better to increase productivity and avoid problems. The Sakura is much faster than the Leica coverslipper. Ren? J. ________________________________ From: "Huggins, Haley - MRMC" To: Rene J Buesa ; "histonet@lists.utsouthwestern.edu" Sent: Thursday, September 12, 2013 11:55 AM Subject: RE: [Histonet] Equipment selection I actually know all the equipment. Well, I know 3 of the pieces really well, the Leica Bond Max is new to me. I did go to another lab to see it as well as the Leica stainer/coverslipper. I was impressed with the Bond Max, not as much with the coverslipper part of the stainer/coverslipper. The big issue I am having is if I want to go with the Bond Max, I technically need to go with the Leica stainer/coverslipper because it is lumped into a big package deal. If I want the Sakura Prisma stainer/film coverslipper, I go with the Dako immunostainer. I am having trouble deciding. It is boiling down to money, which sucks since I might be forced to go with something that I might not be happy with in the future. ? Haley ? From:Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: Thursday, September 12, 2013 5:32 AM To: Huggins, Haley - MRMC; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Equipment selection ? I used the Sakura and the Dako, but it seems that the Leica Bond Max is getting popularity. Why don't you? go to a lab where they are in use and take a look? Ren?J. ? From:"Huggins, Haley - MRMC" To: "histonet@lists.utsouthwestern.edu" Sent: Wednesday, September 11, 2013 4:46 PM Subject: [Histonet] Equipment selection Hi all, I am trying to decide on what equipment to purchase. I have picked the processor, microtomes, embedding centers and a few other pieces, but now I still need to decide on the H&E Stainer and the immunostainer. My options I have in front of me are for the H&E stainer are Sakura Prisma stainer/film coverslipper and the Leica ST5020-CV5030 stainer/glass coverslipper. For the Immunostainer, I am looking at the Dako Autostainer and the Leica Bond Max. Any advice on any of these instruments would be greatly appreciated. I am trying to finalize my decision pretty quickly. Thank you in advance for any input. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Haley.Huggins <@t> DignityHealth.org Thu Sep 12 11:10:47 2013 From: Haley.Huggins <@t> DignityHealth.org (Huggins, Haley - MRMC) Date: Thu Sep 12 11:10:55 2013 Subject: [Histonet] Equipment selection In-Reply-To: <1379002076.53612.YahooMailNeo@web163105.mail.bf1.yahoo.com> References: <4F36EC93A5737D4F8A2974E8FB8E260615F5698BE8@PHX-MSG-007-N2.chw.edu> <1378989146.57715.YahooMailNeo@web163104.mail.bf1.yahoo.com> <4F36EC93A5737D4F8A2974E8FB8E260615F5698C69@PHX-MSG-007-N2.chw.edu> <1379002076.53612.YahooMailNeo@web163105.mail.bf1.yahoo.com> Message-ID: <4F36EC93A5737D4F8A2974E8FB8E260615F5698C6D@PHX-MSG-007-N2.chw.edu> Thanks, that is a good point. From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: Thursday, September 12, 2013 9:08 AM To: Huggins, Haley - MRMC; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Equipment selection If buying the Bond means that you are forced to "swallow" the Leica coverslipper go with Sakura and Dako. If they are making that "package" is because the Bond sells well but they are having to "swallow" the coverslipper. Price is important but in the long run it is better to increase productivity and avoid problems. The Sakura is much faster than the Leica coverslipper. Ren? J. From: "Huggins, Haley - MRMC" > To: Rene J Buesa >; "histonet@lists.utsouthwestern.edu" > Sent: Thursday, September 12, 2013 11:55 AM Subject: RE: [Histonet] Equipment selection I actually know all the equipment. Well, I know 3 of the pieces really well, the Leica Bond Max is new to me. I did go to another lab to see it as well as the Leica stainer/coverslipper. I was impressed with the Bond Max, not as much with the coverslipper part of the stainer/coverslipper. The big issue I am having is if I want to go with the Bond Max, I technically need to go with the Leica stainer/coverslipper because it is lumped into a big package deal. If I want the Sakura Prisma stainer/film coverslipper, I go with the Dako immunostainer. I am having trouble deciding. It is boiling down to money, which sucks since I might be forced to go with something that I might not be happy with in the future. Haley From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: Thursday, September 12, 2013 5:32 AM To: Huggins, Haley - MRMC; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Equipment selection I used the Sakura and the Dako, but it seems that the Leica Bond Max is getting popularity. Why don't you go to a lab where they are in use and take a look? Ren? J. From: "Huggins, Haley - MRMC" > To: "histonet@lists.utsouthwestern.edu" > Sent: Wednesday, September 11, 2013 4:46 PM Subject: [Histonet] Equipment selection Hi all, I am trying to decide on what equipment to purchase. I have picked the processor, microtomes, embedding centers and a few other pieces, but now I still need to decide on the H&E Stainer and the immunostainer. My options I have in front of me are for the H&E stainer are Sakura Prisma stainer/film coverslipper and the Leica ST5020-CV5030 stainer/glass coverslipper. For the Immunostainer, I am looking at the Dako Autostainer and the Leica Bond Max. Any advice on any of these instruments would be greatly appreciated. I am trying to finalize my decision pretty quickly. Thank you in advance for any input. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PAMarcum <@t> uams.edu Thu Sep 12 11:30:13 2013 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Thu Sep 12 11:30:20 2013 Subject: [Histonet] Equipment selection In-Reply-To: <1379002076.53612.YahooMailNeo@web163105.mail.bf1.yahoo.com> References: <4F36EC93A5737D4F8A2974E8FB8E260615F5698BE8@PHX-MSG-007-N2.chw.edu> <1378989146.57715.YahooMailNeo@web163104.mail.bf1.yahoo.com> <4F36EC93A5737D4F8A2974E8FB8E260615F5698C69@PHX-MSG-007-N2.chw.edu> <1379002076.53612.YahooMailNeo@web163105.mail.bf1.yahoo.com> Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA32D3158B57@Mail2Node2.ad.uams.edu> Actually the coverslipper from Leica is very good and can be linked to the stainer. It is also a glass coverslipper not tape. It is always about money and that may be the thing that decides no matter what any of prefers. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, September 12, 2013 11:08 AM To: Huggins, Haley - MRMC; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Equipment selection If buying the Bond means that you are forced to "swallow" the Leica coverslipper go with Sakura and Dako. If they are making that "package" is because the Bond sells well?but they are having to "swallow" the coverslipper. Price is important but in the long run it is better to increase productivity and avoid problems. The Sakura is much faster than the Leica coverslipper. Ren? J. ________________________________ From: "Huggins, Haley - MRMC" To: Rene J Buesa ; "histonet@lists.utsouthwestern.edu" Sent: Thursday, September 12, 2013 11:55 AM Subject: RE: [Histonet] Equipment selection I actually know all the equipment. Well, I know 3 of the pieces really well, the Leica Bond Max is new to me. I did go to another lab to see it as well as the Leica stainer/coverslipper. I was impressed with the Bond Max, not as much with the coverslipper part of the stainer/coverslipper. The big issue I am having is if I want to go with the Bond Max, I technically need to go with the Leica stainer/coverslipper because it is lumped into a big package deal. If I want the Sakura Prisma stainer/film coverslipper, I go with the Dako immunostainer. I am having trouble deciding. It is boiling down to money, which sucks since I might be forced to go with something that I might not be happy with in the future. ? Haley ? From:Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: Thursday, September 12, 2013 5:32 AM To: Huggins, Haley - MRMC; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Equipment selection ? I used the Sakura and the Dako, but it seems that the Leica Bond Max is getting popularity. Why don't you? go to a lab where they are in use and take a look? Ren?J. ? From:"Huggins, Haley - MRMC" To: "histonet@lists.utsouthwestern.edu" Sent: Wednesday, September 11, 2013 4:46 PM Subject: [Histonet] Equipment selection Hi all, I am trying to decide on what equipment to purchase. I have picked the processor, microtomes, embedding centers and a few other pieces, but now I still need to decide on the H&E Stainer and the immunostainer. My options I have in front of me are for the H&E stainer are Sakura Prisma stainer/film coverslipper and the Leica ST5020-CV5030 stainer/glass coverslipper. For the Immunostainer, I am looking at the Dako Autostainer and the Leica Bond Max. Any advice on any of these instruments would be greatly appreciated. I am trying to finalize my decision pretty quickly. Thank you in advance for any input. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From Dorothy.L.Webb <@t> HealthPartners.Com Thu Sep 12 11:45:41 2013 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Thu Sep 12 11:45:50 2013 Subject: [Histonet] Need your expertise Message-ID: <65365F35C0F2EF4D846EC3CA73E49C4302ACEFF2C823@HPEMX3.HealthPartners.int> We will finally be going green by recycling formalin. I would appreciate any advice or opinions on true recycling VS. a filtering type of system. As we all know, experience with a system or product is the best way to gain true knowledge! Also, I would appreciate knowing how others are handling extra levels of prostate needle bx's. We currently place them on charged slides and keep until the case is signed out, but it seems there should be a better way of handling this due to storage of the slides not to mention the extra cost involved! I know this is becoming more of a needed process with all of the RNA/DNA testing being done on diagnosed cancers. As always, I do appreciate any and all comments, advice, or words of wisdom!! Hope to see some of you in Providence late next week! Dorothy Webb, HT (ASCP) Regions Histology Technical Specialist 651-254-2962 ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 From thigginsht <@t> msn.com Thu Sep 12 11:55:43 2013 From: thigginsht <@t> msn.com (Tim Higgins) Date: Thu Sep 12 11:55:47 2013 Subject: [Histonet] RE: Histonet Digest, Vol 118, Issue 23 In-Reply-To: References: Message-ID: Hey Martha, You have to forgive Rene J. she speaks sometimes and has nothing good to say but keeps speaking. There is never a bad question, if you don't have a good answer then keep it to yourself as I usually do. "Adequate forum". really? I guess no one on Histonet is allowed to share their personal protocols, procedures, opinions, and experiences on here anymore. Come on Rene J., next time shhhhhhhhh!! I should have just hit the "Delete" button and follow my own advise! Tim N. Higgins, HT (ASCP), QIHC > Message: 7 > Date: Thu, 12 Sep 2013 06:22:34 -0700 (PDT) > From: Rene J Buesa > Subject: Re: [Histonet] Minimum sample size for breast markers > To: Martha Ward-Pathology , > "histonet@lists.utsouthwestern.edu" > > Message-ID: > <1378992154.20733.YahooMailNeo@web163104.mail.bf1.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > I do not think this is the adequate forum to ask this question because you may get a wrong answer that could determine your decision. > Your pathologist is the one who should decide about this issue. > Ren? J. > > > ________________________________ > From: Martha Ward-Pathology > To: "histonet@lists.utsouthwestern.edu" > Sent: Thursday, September 12, 2013 9:06 AM > Subject: [Histonet] Minimum sample size for breast markers > > > > > Is there a minimum number of cells that should be present when testing for ER, PR and Her2? I have looked on the CAP website but cannot see anything about this. We are constantly getting these small biopsies or FNAs for testing and I sometimes wonder what the lowest threshold is for testing...20 tumor cells, 50 tumor cells???? Any information would be appreciated. Thanks in advance for your help. > > Martha Ward, MT (ASCP) QIHC > Manager > > Molecular Diagnostics Lab > Medical Center Boulevard \ Winston-Salem, NC 27157 > p 336.716.2109 \ f 336.716.5890 > mward@wakehealth.edu > From estellamireles <@t> gmail.com Thu Sep 12 12:15:18 2013 From: estellamireles <@t> gmail.com (Stella Mireles) Date: Thu Sep 12 12:15:25 2013 Subject: [Histonet] Fat Stain on Paraffin Slide ? Message-ID: Ok Experts - Please advise me on a stain for Fat which I can do on paraffin sections? Could you expand on your own experience with such an animal. Thanks to All Stella Houston, Tx From madeleinehuey <@t> gmail.com Thu Sep 12 12:18:02 2013 From: madeleinehuey <@t> gmail.com (Madeleine Huey) Date: Thu Sep 12 12:18:09 2013 Subject: [Histonet] Re: Histonet Digest, Vol 118, Issue 24 In-Reply-To: <5231f38d.e9363c0a.4773.ffffb744SMTPIN_ADDED_MISSING@mx.google.com> References: <5231f38d.e9363c0a.4773.ffffb744SMTPIN_ADDED_MISSING@mx.google.com> Message-ID: From:"Huggins, Haley - MRMC" To: "histonet@lists.utsouthwestern.edu" Sent: Wednesday, September 11, 2013 4:46 PM Subject: [Histonet] Equipment selection Hi all, I am trying to decide on what equipment to purchase. I have picked the processor, microtomes, embedding centers and a few other pieces, but now I still need to decide on the H&E Stainer and the immunostainer. My options I have in front of me are for the H&E stainer are Sakura Prisma stainer/film coverslipper and the Leica ST5020-CV5030 stainer/glass coverslipper. For the Immunostainer, I am looking at the Dako Autostainer and the Leica Bond Max. Any advice on any of these instruments would be greatly appreciated. I am trying to finalize my decision pretty quickly. Thank you in advance for any input. Haley, I have experiences with all the above equipments and here's my inf; 1) Leica H&E autostainer with glass coverslipper - smaller size (use lesser counter space) cheaper (buy 2 equivalent to 2 Sakura Prisma + film coverslipper and smaller. On Thu, Sep 12, 2013 at 10:02 AM, wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. RE: Equipment selection (Huggins, Haley - MRMC) > 2. Re: Equipment selection (Rene J Buesa) > 3. RE: Equipment selection (Huggins, Haley - MRMC) > 4. RE: Equipment selection (Marcum, Pamela A) > 5. Need your expertise (Webb, Dorothy L) > 6. RE: Histonet Digest, Vol 118, Issue 23 (Tim Higgins) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Thu, 12 Sep 2013 08:55:39 -0700 > From: "Huggins, Haley - MRMC" > Subject: RE: [Histonet] Equipment selection > To: Rene J Buesa , > "histonet@lists.utsouthwestern.edu" > > Message-ID: > <4F36EC93A5737D4F8A2974E8FB8E260615F5698C69@PHX-MSG-007-N2.chw.edu > > > Content-Type: text/plain; charset="iso-8859-1" > > I actually know all the equipment. Well, I know 3 of the pieces really > well, the Leica Bond Max is new to me. I did go to another lab to see it as > well as the Leica stainer/coverslipper. I was impressed with the Bond Max, > not as much with the coverslipper part of the stainer/coverslipper. The big > issue I am having is if I want to go with the Bond Max, I technically need > to go with the Leica stainer/coverslipper because it is lumped into a big > package deal. If I want the Sakura Prisma stainer/film coverslipper, I go > with the Dako immunostainer. I am having trouble deciding. It is boiling > down to money, which sucks since I might be forced to go with something > that I might not be happy with in the future. > > Haley > > From: Rene J Buesa [mailto:rjbuesa@yahoo.com] > Sent: Thursday, September 12, 2013 5:32 AM > To: Huggins, Haley - MRMC; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Equipment selection > > I used the Sakura and the Dako, but it seems that the Leica Bond Max is > getting popularity. Why don't you go to a lab where they are in use and > take a look? > Ren? J. > > From: "Huggins, Haley - MRMC" Haley.Huggins@DignityHealth.org>> > To: "histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu>" > > Sent: Wednesday, September 11, 2013 4:46 PM > Subject: [Histonet] Equipment selection > > Hi all, > > I am trying to decide on what equipment to purchase. I have picked the > processor, microtomes, embedding centers and a few other pieces, but now I > still need to decide on the H&E Stainer and the immunostainer. My options I > have in front of me are for the H&E stainer are Sakura Prisma stainer/film > coverslipper and the Leica ST5020-CV5030 stainer/glass coverslipper. For > the Immunostainer, I am looking at the Dako Autostainer and the Leica Bond > Max. Any advice on any of these instruments would be greatly appreciated. I > am trying to finalize my decision pretty quickly. Thank you in advance for > any input. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Message: 2 > Date: Thu, 12 Sep 2013 09:07:56 -0700 (PDT) > From: Rene J Buesa > Subject: Re: [Histonet] Equipment selection > To: "Huggins, Haley - MRMC" , > "histonet@lists.utsouthwestern.edu" > > Message-ID: > <1379002076.53612.YahooMailNeo@web163105.mail.bf1.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > If buying the Bond means that you are forced to "swallow" the Leica > coverslipper go with Sakura and Dako. > If they are making that "package" is because the Bond sells well but they > are having to "swallow" the coverslipper. Price is important but in the > long run it is better to increase productivity and avoid problems. The > Sakura is much faster than the Leica coverslipper. > Ren? J. > > > ________________________________ > From: "Huggins, Haley - MRMC" > To: Rene J Buesa ; "histonet@lists.utsouthwestern.edu" > > Sent: Thursday, September 12, 2013 11:55 AM > Subject: RE: [Histonet] Equipment selection > > > > I actually know all the equipment. Well, I know 3 of the pieces really > well, the Leica Bond Max is new to me. I did go to another lab to see it as > well as the Leica stainer/coverslipper. I was impressed with the Bond Max, > not as much with the coverslipper part of the stainer/coverslipper. The big > issue I am having is if I want to go with the Bond Max, I technically need > to go with the Leica stainer/coverslipper because it is lumped into a big > package deal. If I want the Sakura Prisma stainer/film coverslipper, I go > with the Dako immunostainer. I am having trouble deciding. It is boiling > down to money, which sucks since I might be forced to go with something > that I might not be happy with in the future. > > Haley > > From:Rene J Buesa [mailto:rjbuesa@yahoo.com] > Sent: Thursday, September 12, 2013 5:32 AM > To: Huggins, Haley - MRMC; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Equipment selection > > I used the Sakura and the Dako, but it seems that the Leica Bond Max is > getting popularity. Why don't you go to a lab where they are in use and > take a look? > Ren?J. > > From:"Huggins, Haley - MRMC" > To: "histonet@lists.utsouthwestern.edu" > > Sent: Wednesday, September 11, 2013 4:46 PM > Subject: [Histonet] Equipment selection > > Hi all, > > I am trying to decide on what equipment to purchase. I have picked the > processor, microtomes, embedding centers and a few other pieces, but now I > still need to decide on the H&E Stainer and the immunostainer. My options I > have in front of me are for the H&E stainer are Sakura Prisma stainer/film > coverslipper and the Leica ST5020-CV5030 stainer/glass coverslipper. For > the Immunostainer, I am looking at the Dako Autostainer and the Leica Bond > Max. Any advice on any of these instruments would be greatly appreciated. I > am trying to finalize my decision pretty quickly. Thank you in advance for > any input. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > Message: 3 > Date: Thu, 12 Sep 2013 09:10:47 -0700 > From: "Huggins, Haley - MRMC" > Subject: RE: [Histonet] Equipment selection > To: Rene J Buesa , > "histonet@lists.utsouthwestern.edu" > > Message-ID: > <4F36EC93A5737D4F8A2974E8FB8E260615F5698C6D@PHX-MSG-007-N2.chw.edu > > > Content-Type: text/plain; charset="iso-8859-1" > > Thanks, that is a good point. > > From: Rene J Buesa [mailto:rjbuesa@yahoo.com] > Sent: Thursday, September 12, 2013 9:08 AM > To: Huggins, Haley - MRMC; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Equipment selection > > If buying the Bond means that you are forced to "swallow" the Leica > coverslipper go with Sakura and Dako. > If they are making that "package" is because the Bond sells well but they > are having to "swallow" the coverslipper. Price is important but in the > long run it is better to increase productivity and avoid problems. The > Sakura is much faster than the Leica coverslipper. > Ren? J. > > From: "Huggins, Haley - MRMC" Haley.Huggins@DignityHealth.org>> > To: Rene J Buesa >; " > histonet@lists.utsouthwestern.edu" > histonet@lists.utsouthwestern.edu>> > Sent: Thursday, September 12, 2013 11:55 AM > Subject: RE: [Histonet] Equipment selection > > I actually know all the equipment. Well, I know 3 of the pieces really > well, the Leica Bond Max is new to me. I did go to another lab to see it as > well as the Leica stainer/coverslipper. I was impressed with the Bond Max, > not as much with the coverslipper part of the stainer/coverslipper. The big > issue I am having is if I want to go with the Bond Max, I technically need > to go with the Leica stainer/coverslipper because it is lumped into a big > package deal. If I want the Sakura Prisma stainer/film coverslipper, I go > with the Dako immunostainer. I am having trouble deciding. It is boiling > down to money, which sucks since I might be forced to go with something > that I might not be happy with in the future. > > Haley > > From: Rene J Buesa [mailto:rjbuesa@yahoo.com] > Sent: Thursday, September 12, 2013 5:32 AM > To: Huggins, Haley - MRMC; histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu> > Subject: Re: [Histonet] Equipment selection > > I used the Sakura and the Dako, but it seems that the Leica Bond Max is > getting popularity. Why don't you go to a lab where they are in use and > take a look? > Ren? J. > > From: "Huggins, Haley - MRMC" Haley.Huggins@DignityHealth.org>> > To: "histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu>" > > Sent: Wednesday, September 11, 2013 4:46 PM > Subject: [Histonet] Equipment selection > > Hi all, > > I am trying to decide on what equipment to purchase. I have picked the > processor, microtomes, embedding centers and a few other pieces, but now I > still need to decide on the H&E Stainer and the immunostainer. My options I > have in front of me are for the H&E stainer are Sakura Prisma stainer/film > coverslipper and the Leica ST5020-CV5030 stainer/glass coverslipper. For > the Immunostainer, I am looking at the Dako Autostainer and the Leica Bond > Max. Any advice on any of these instruments would be greatly appreciated. I > am trying to finalize my decision pretty quickly. Thank you in advance for > any input. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 4 > Date: Thu, 12 Sep 2013 16:30:13 +0000 > From: "Marcum, Pamela A" > Subject: RE: [Histonet] Equipment selection > To: "'Rene J Buesa'" , "Huggins, Haley - MRMC" > , " > histonet@lists.utsouthwestern.edu" > > Message-ID: > <41D3A1AF6FEF0643BDC89E0516A6EA32D3158B57@Mail2Node2.ad.uams.edu> > Content-Type: text/plain; charset="iso-8859-1" > > Actually the coverslipper from Leica is very good and can be linked to the > stainer. It is also a glass coverslipper not tape. It is always about > money and that may be the thing that decides no matter what any of prefers. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa > Sent: Thursday, September 12, 2013 11:08 AM > To: Huggins, Haley - MRMC; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Equipment selection > > If buying the Bond means that you are forced to "swallow" the Leica > coverslipper go with Sakura and Dako. > If they are making that "package" is because the Bond sells well but they > are having to "swallow" the coverslipper. Price is important but in the > long run it is better to increase productivity and avoid problems. The > Sakura is much faster than the Leica coverslipper. > Ren? J. > > > ________________________________ > From: "Huggins, Haley - MRMC" > To: Rene J Buesa ; "histonet@lists.utsouthwestern.edu" > > Sent: Thursday, September 12, 2013 11:55 AM > Subject: RE: [Histonet] Equipment selection > > > > I actually know all the equipment. Well, I know 3 of the pieces really > well, the Leica Bond Max is new to me. I did go to another lab to see it as > well as the Leica stainer/coverslipper. I was impressed with the Bond Max, > not as much with the coverslipper part of the stainer/coverslipper. The big > issue I am having is if I want to go with the Bond Max, I technically need > to go with the Leica stainer/coverslipper because it is lumped into a big > package deal. If I want the Sakura Prisma stainer/film coverslipper, I go > with the Dako immunostainer. I am having trouble deciding. It is boiling > down to money, which sucks since I might be forced to go with something > that I might not be happy with in the future. > > Haley > > From:Rene J Buesa [mailto:rjbuesa@yahoo.com] > Sent: Thursday, September 12, 2013 5:32 AM > To: Huggins, Haley - MRMC; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Equipment selection > > I used the Sakura and the Dako, but it seems that the Leica Bond Max is > getting popularity. Why don't you go to a lab where they are in use and > take a look? > Ren?J. > > From:"Huggins, Haley - MRMC" > To: "histonet@lists.utsouthwestern.edu" > > Sent: Wednesday, September 11, 2013 4:46 PM > Subject: [Histonet] Equipment selection > > Hi all, > > I am trying to decide on what equipment to purchase. I have picked the > processor, microtomes, embedding centers and a few other pieces, but now I > still need to decide on the H&E Stainer and the immunostainer. My options I > have in front of me are for the H&E stainer are Sakura Prisma stainer/film > coverslipper and the Leica ST5020-CV5030 stainer/glass coverslipper. For > the Immunostainer, I am looking at the Dako Autostainer and the Leica Bond > Max. Any advice on any of these instruments would be greatly appreciated. I > am trying to finalize my decision pretty quickly. Thank you in advance for > any input. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ---------------------------------------------------------------------- > Confidentiality Notice: This e-mail message, including any attachments, is > for the sole use of the intended recipient(s) and may contain confidential > and privileged information. Any unauthorized review, use, disclosure or > distribution is prohibited. If you are not the intended recipient, please > contact the sender by reply e-mail and destroy all copies of the original > message. > > > > ------------------------------ > > Message: 5 > Date: Thu, 12 Sep 2013 11:45:41 -0500 > From: "Webb, Dorothy L" > Subject: [Histonet] Need your expertise > To: "'histonet@lists.utsouthwestern.edu'" > > Message-ID: > < > 65365F35C0F2EF4D846EC3CA73E49C4302ACEFF2C823@HPEMX3.HealthPartners.int> > > Content-Type: text/plain; charset="us-ascii" > > We will finally be going green by recycling formalin. I would appreciate > any advice or opinions on true recycling VS. a filtering type of system. As > we all know, experience with a system or product is the best way to gain > true knowledge! > > Also, I would appreciate knowing how others are handling extra levels of > prostate needle bx's. We currently place them on charged slides and keep > until the case is signed out, but it seems there should be a better way of > handling this due to storage of the slides not to mention the extra cost > involved! I know this is becoming more of a needed process with all of the > RNA/DNA testing being done on diagnosed cancers. > > As always, I do appreciate any and all comments, advice, or words of > wisdom!! Hope to see some of you in Providence late next week! > > Dorothy Webb, HT (ASCP) > Regions Histology Technical Specialist > 651-254-2962 > > > > ________________________________ > This e-mail and any files transmitted with it are confidential and are > intended solely for the use of the individual or entity to whom they are > addressed. If you are not the intended recipient or the individual > responsible for delivering the e-mail to the intended recipient, please be > advised that you have received this e-mail in error and that any use, > dissemination, forwarding, printing, or copying of this e-mail is strictly > prohibited. > > If you have received this communication in error, please return it to the > sender immediately and delete the original message and any copy of it from > your computer system. If you have any questions concerning this message, > please contact the sender. Disclaimer R001.0 > > > ------------------------------ > > Message: 6 > Date: Thu, 12 Sep 2013 11:55:43 -0500 > From: Tim Higgins > Subject: [Histonet] RE: Histonet Digest, Vol 118, Issue 23 > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > Hey Martha, > > You have to forgive Rene J. she speaks sometimes and has nothing good to > say but keeps speaking. There is never a bad question, if you don't have a > good answer then keep it to yourself as I usually do. > > "Adequate forum". really? I guess no one on Histonet is allowed to share > their personal protocols, procedures, opinions, and experiences on here > anymore. > > Come on Rene J., next time shhhhhhhhh!! > > I should have just hit the "Delete" button and follow my own advise! > > Tim N. Higgins, HT (ASCP), QIHC > > > > Message: 7 > > Date: Thu, 12 Sep 2013 06:22:34 -0700 (PDT) > > From: Rene J Buesa > > Subject: Re: [Histonet] Minimum sample size for breast markers > > To: Martha Ward-Pathology , > > "histonet@lists.utsouthwestern.edu" > > > > Message-ID: > > <1378992154.20733.YahooMailNeo@web163104.mail.bf1.yahoo.com> > > Content-Type: text/plain; charset=iso-8859-1 > > > > I do not think this is the adequate forum to ask this question because > you may get a wrong answer that could determine your decision. > > Your pathologist is the one who should decide about this issue. > > Ren? J. > > > > > > ________________________________ > > From: Martha Ward-Pathology > > To: "histonet@lists.utsouthwestern.edu" < > histonet@lists.utsouthwestern.edu> > > Sent: Thursday, September 12, 2013 9:06 AM > > Subject: [Histonet] Minimum sample size for breast markers > > > > > > > > > > Is there a minimum number of cells that should be present when testing > for ER, PR and Her2? I have looked on the CAP website but cannot see > anything about this. We are constantly getting these small biopsies or > FNAs for testing and I sometimes wonder what the lowest threshold is for > testing...20 tumor cells, 50 tumor cells???? Any information would be > appreciated. Thanks in advance for your help. > > > > Martha Ward, MT (ASCP) QIHC > > Manager > > > > Molecular Diagnostics Lab > > Medical Center Boulevard \ Winston-Salem, NC 27157 > > p 336.716.2109 \ f 336.716.5890 > > mward@wakehealth.edu > > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 118, Issue 24 > ***************************************** > From CDavis <@t> che-east.org Thu Sep 12 12:27:10 2013 From: CDavis <@t> che-east.org (Davis, Cassie) Date: Thu Sep 12 12:27:21 2013 Subject: [Histonet] Sakura Prisma stainer/film coverslipper In-Reply-To: References: Message-ID: <08861B9CF6C7774E874635A4818AE37B0125D3213C@CHEXCMS01.one.ads.che.org> Hi Haley, we've been using this H & E stainer and tape coverslipper for about 5 years with pretty good results even though we do not have the piece to link the two together. The very few problems we have had are related to lack of daily maintenance which is minimal. We are in the process of "handing it down" to cytology and trying to use the new Symphony for H &E because of the tracking system option. The folks that do the microtomy are not happy because using the trays are more time consuming due to the "learning curve" (we are told), which is the trade-off. I hope this helps. Cassandra Davis CDavis@che-east.org 302-575-8095 ________________________________________ From: "Huggins, Haley - MRMC" > To: "histonet@lists.utsouthwestern.edu" > Sent: Wednesday, September 11, 2013 4:46 PM Subject: [Histonet] Equipment selection Hi all, I am trying to decide on what equipment to purchase. I have picked the processor, microtomes, embedding centers and a few other pieces, but now I still need to decide on the H&E Stainer and the immunostainer. My options I have in front of me are for the H&E stainer are Sakura Prisma stainer/film coverslipper and the Leica ST5020-CV5030 stainer/glass coverslipper. For the Immunostainer, I am looking at the Dako Autostainer and the Leica Bond Max. Any advice on any of these instruments would be greatly appreciated. I am trying to finalize my decision pretty quickly. Thank you in advance for any input. Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From madeleinehuey <@t> gmail.com Thu Sep 12 12:34:57 2013 From: madeleinehuey <@t> gmail.com (Madeleine Huey) Date: Thu Sep 12 12:35:05 2013 Subject: [Histonet] Re: Histonet Digest, Vol 118, Issue 24 In-Reply-To: References: <5231f38d.e9363c0a.4773.ffffb744SMTPIN_ADDED_MISSING@mx.google.com> Message-ID: O..o.oop! PC problem. Haley, I have experiences with all the above equipment?s and here's my inf; 1) Leica H&E Autostainer + glass coverslipper - smaller size therefore use lesser counter space 2) Lesser cost $$ for Leica HE Autostainer / coverslipper 3) Bond Max/Bond III could do IHC & ISH simultaneously in one Autostainer, while DAKO IHC autostainer can do only IHC (no heating elements) 4) Bond can do DELAY start while you are sleeping & continuos flow as well, while DAKO need removed slides after AR & run immediately (batches) Madeleine Huey BS, HTL/QIHC (ASCP) Supervisor - IHC & Histology (Pathology) On Thu, Sep 12, 2013 at 10:18 AM, Madeleine Huey wrote: > From:"Huggins, Haley - MRMC" > To: "histonet@lists.utsouthwestern.edu" > > Sent: Wednesday, September 11, 2013 4:46 PM > Subject: [Histonet] Equipment selection > > Hi all, > > I am trying to decide on what equipment to purchase. I have picked the > processor, microtomes, embedding centers and a few other pieces, but now I > still need to decide on the H&E Stainer and the immunostainer. My options I > have in front of me are for the H&E stainer are Sakura Prisma stainer/film > coverslipper and the Leica ST5020-CV5030 stainer/glass coverslipper. For > the Immunostainer, I am looking at the Dako Autostainer and the Leica Bond > Max. Any advice on any of these instruments would be greatly appreciated. I > am trying to finalize my decision pretty quickly. Thank you in advance for > any input. > > Haley, > I have experiences with all the above equipments and here's my inf; > 1) Leica H&E autostainer with glass coverslipper - smaller size (use > lesser counter space) > cheaper (buy 2 equivalent to 2 Sakura Prisma + film coverslipper and > smaller. > > > On Thu, Sep 12, 2013 at 10:02 AM, < > histonet-request@lists.utsouthwestern.edu> wrote: > >> Send Histonet mailing list submissions to >> histonet@lists.utsouthwestern.edu >> >> To subscribe or unsubscribe via the World Wide Web, visit >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> or, via email, send a message with subject or body 'help' to >> histonet-request@lists.utsouthwestern.edu >> >> You can reach the person managing the list at >> histonet-owner@lists.utsouthwestern.edu >> >> When replying, please edit your Subject line so it is more specific >> than "Re: Contents of Histonet digest..." >> >> >> Today's Topics: >> >> 1. RE: Equipment selection (Huggins, Haley - MRMC) >> 2. Re: Equipment selection (Rene J Buesa) >> 3. RE: Equipment selection (Huggins, Haley - MRMC) >> 4. RE: Equipment selection (Marcum, Pamela A) >> 5. Need your expertise (Webb, Dorothy L) >> 6. RE: Histonet Digest, Vol 118, Issue 23 (Tim Higgins) >> >> >> ---------------------------------------------------------------------- >> >> Message: 1 >> Date: Thu, 12 Sep 2013 08:55:39 -0700 >> From: "Huggins, Haley - MRMC" >> Subject: RE: [Histonet] Equipment selection >> To: Rene J Buesa , >> "histonet@lists.utsouthwestern.edu" >> >> Message-ID: >> < >> 4F36EC93A5737D4F8A2974E8FB8E260615F5698C69@PHX-MSG-007-N2.chw.edu> >> Content-Type: text/plain; charset="iso-8859-1" >> >> I actually know all the equipment. Well, I know 3 of the pieces really >> well, the Leica Bond Max is new to me. I did go to another lab to see it as >> well as the Leica stainer/coverslipper. I was impressed with the Bond Max, >> not as much with the coverslipper part of the stainer/coverslipper. The big >> issue I am having is if I want to go with the Bond Max, I technically need >> to go with the Leica stainer/coverslipper because it is lumped into a big >> package deal. If I want the Sakura Prisma stainer/film coverslipper, I go >> with the Dako immunostainer. I am having trouble deciding. It is boiling >> down to money, which sucks since I might be forced to go with something >> that I might not be happy with in the future. >> >> Haley >> >> From: Rene J Buesa [mailto:rjbuesa@yahoo.com] >> Sent: Thursday, September 12, 2013 5:32 AM >> To: Huggins, Haley - MRMC; histonet@lists.utsouthwestern.edu >> Subject: Re: [Histonet] Equipment selection >> >> I used the Sakura and the Dako, but it seems that the Leica Bond Max is >> getting popularity. Why don't you go to a lab where they are in use and >> take a look? >> Ren? J. >> >> From: "Huggins, Haley - MRMC" > Haley.Huggins@DignityHealth.org>> >> To: "histonet@lists.utsouthwestern.edu> histonet@lists.utsouthwestern.edu>" > > >> Sent: Wednesday, September 11, 2013 4:46 PM >> Subject: [Histonet] Equipment selection >> >> Hi all, >> >> I am trying to decide on what equipment to purchase. I have picked the >> processor, microtomes, embedding centers and a few other pieces, but now I >> still need to decide on the H&E Stainer and the immunostainer. My options I >> have in front of me are for the H&E stainer are Sakura Prisma stainer/film >> coverslipper and the Leica ST5020-CV5030 stainer/glass coverslipper. For >> the Immunostainer, I am looking at the Dako Autostainer and the Leica Bond >> Max. Any advice on any of these instruments would be greatly appreciated. I >> am trying to finalize my decision pretty quickly. Thank you in advance for >> any input. >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu> Histonet@lists.utsouthwestern.edu> >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> ------------------------------ >> >> Message: 2 >> Date: Thu, 12 Sep 2013 09:07:56 -0700 (PDT) >> From: Rene J Buesa >> Subject: Re: [Histonet] Equipment selection >> To: "Huggins, Haley - MRMC" , >> "histonet@lists.utsouthwestern.edu" >> >> Message-ID: >> <1379002076.53612.YahooMailNeo@web163105.mail.bf1.yahoo.com> >> Content-Type: text/plain; charset=iso-8859-1 >> >> If buying the Bond means that you are forced to "swallow" the Leica >> coverslipper go with Sakura and Dako. >> If they are making that "package" is because the Bond sells well but they >> are having to "swallow" the coverslipper. Price is important but in the >> long run it is better to increase productivity and avoid problems. The >> Sakura is much faster than the Leica coverslipper. >> Ren? J. >> >> >> ________________________________ >> From: "Huggins, Haley - MRMC" >> To: Rene J Buesa ; "histonet@lists.utsouthwestern.edu" >> >> Sent: Thursday, September 12, 2013 11:55 AM >> Subject: RE: [Histonet] Equipment selection >> >> >> >> I actually know all the equipment. Well, I know 3 of the pieces really >> well, the Leica Bond Max is new to me. I did go to another lab to see it as >> well as the Leica stainer/coverslipper. I was impressed with the Bond Max, >> not as much with the coverslipper part of the stainer/coverslipper. The big >> issue I am having is if I want to go with the Bond Max, I technically need >> to go with the Leica stainer/coverslipper because it is lumped into a big >> package deal. If I want the Sakura Prisma stainer/film coverslipper, I go >> with the Dako immunostainer. I am having trouble deciding. It is boiling >> down to money, which sucks since I might be forced to go with something >> that I might not be happy with in the future. >> >> Haley >> >> From:Rene J Buesa [mailto:rjbuesa@yahoo.com] >> Sent: Thursday, September 12, 2013 5:32 AM >> To: Huggins, Haley - MRMC; histonet@lists.utsouthwestern.edu >> Subject: Re: [Histonet] Equipment selection >> >> I used the Sakura and the Dako, but it seems that the Leica Bond Max is >> getting popularity. Why don't you go to a lab where they are in use and >> take a look? >> Ren?J. >> >> From:"Huggins, Haley - MRMC" >> To: "histonet@lists.utsouthwestern.edu" < >> histonet@lists.utsouthwestern.edu> >> Sent: Wednesday, September 11, 2013 4:46 PM >> Subject: [Histonet] Equipment selection >> >> Hi all, >> >> I am trying to decide on what equipment to purchase. I have picked the >> processor, microtomes, embedding centers and a few other pieces, but now I >> still need to decide on the H&E Stainer and the immunostainer. My options I >> have in front of me are for the H&E stainer are Sakura Prisma stainer/film >> coverslipper and the Leica ST5020-CV5030 stainer/glass coverslipper. For >> the Immunostainer, I am looking at the Dako Autostainer and the Leica Bond >> Max. Any advice on any of these instruments would be greatly appreciated. I >> am trying to finalize my decision pretty quickly. Thank you in advance for >> any input. >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> ------------------------------ >> >> Message: 3 >> Date: Thu, 12 Sep 2013 09:10:47 -0700 >> From: "Huggins, Haley - MRMC" >> Subject: RE: [Histonet] Equipment selection >> To: Rene J Buesa , >> "histonet@lists.utsouthwestern.edu" >> >> Message-ID: >> < >> 4F36EC93A5737D4F8A2974E8FB8E260615F5698C6D@PHX-MSG-007-N2.chw.edu> >> Content-Type: text/plain; charset="iso-8859-1" >> >> Thanks, that is a good point. >> >> From: Rene J Buesa [mailto:rjbuesa@yahoo.com] >> Sent: Thursday, September 12, 2013 9:08 AM >> To: Huggins, Haley - MRMC; histonet@lists.utsouthwestern.edu >> Subject: Re: [Histonet] Equipment selection >> >> If buying the Bond means that you are forced to "swallow" the Leica >> coverslipper go with Sakura and Dako. >> If they are making that "package" is because the Bond sells well but they >> are having to "swallow" the coverslipper. Price is important but in the >> long run it is better to increase productivity and avoid problems. The >> Sakura is much faster than the Leica coverslipper. >> Ren? J. >> >> From: "Huggins, Haley - MRMC" > Haley.Huggins@DignityHealth.org>> >> To: Rene J Buesa >; " >> histonet@lists.utsouthwestern.edu> histonet@lists.utsouthwestern.edu>" > > >> Sent: Thursday, September 12, 2013 11:55 AM >> Subject: RE: [Histonet] Equipment selection >> >> I actually know all the equipment. Well, I know 3 of the pieces really >> well, the Leica Bond Max is new to me. I did go to another lab to see it as >> well as the Leica stainer/coverslipper. I was impressed with the Bond Max, >> not as much with the coverslipper part of the stainer/coverslipper. The big >> issue I am having is if I want to go with the Bond Max, I technically need >> to go with the Leica stainer/coverslipper because it is lumped into a big >> package deal. If I want the Sakura Prisma stainer/film coverslipper, I go >> with the Dako immunostainer. I am having trouble deciding. It is boiling >> down to money, which sucks since I might be forced to go with something >> that I might not be happy with in the future. >> >> Haley >> >> From: Rene J Buesa [mailto:rjbuesa@yahoo.com] >> Sent: Thursday, September 12, 2013 5:32 AM >> To: Huggins, Haley - MRMC; histonet@lists.utsouthwestern.edu> histonet@lists.utsouthwestern.edu> >> Subject: Re: [Histonet] Equipment selection >> >> I used the Sakura and the Dako, but it seems that the Leica Bond Max is >> getting popularity. Why don't you go to a lab where they are in use and >> take a look? >> Ren? J. >> >> From: "Huggins, Haley - MRMC" > Haley.Huggins@DignityHealth.org>> >> To: "histonet@lists.utsouthwestern.edu> histonet@lists.utsouthwestern.edu>" > > >> Sent: Wednesday, September 11, 2013 4:46 PM >> Subject: [Histonet] Equipment selection >> >> Hi all, >> >> I am trying to decide on what equipment to purchase. I have picked the >> processor, microtomes, embedding centers and a few other pieces, but now I >> still need to decide on the H&E Stainer and the immunostainer. My options I >> have in front of me are for the H&E stainer are Sakura Prisma stainer/film >> coverslipper and the Leica ST5020-CV5030 stainer/glass coverslipper. For >> the Immunostainer, I am looking at the Dako Autostainer and the Leica Bond >> Max. Any advice on any of these instruments would be greatly appreciated. I >> am trying to finalize my decision pretty quickly. Thank you in advance for >> any input. >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu> Histonet@lists.utsouthwestern.edu> >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> ------------------------------ >> >> Message: 4 >> Date: Thu, 12 Sep 2013 16:30:13 +0000 >> From: "Marcum, Pamela A" >> Subject: RE: [Histonet] Equipment selection >> To: "'Rene J Buesa'" , "Huggins, Haley - MRMC" >> , " >> histonet@lists.utsouthwestern.edu" >> >> Message-ID: >> <41D3A1AF6FEF0643BDC89E0516A6EA32D3158B57@Mail2Node2.ad.uams.edu> >> Content-Type: text/plain; charset="iso-8859-1" >> >> Actually the coverslipper from Leica is very good and can be linked to >> the stainer. It is also a glass coverslipper not tape. It is always about >> money and that may be the thing that decides no matter what any of prefers. >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu [mailto: >> histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa >> Sent: Thursday, September 12, 2013 11:08 AM >> To: Huggins, Haley - MRMC; histonet@lists.utsouthwestern.edu >> Subject: Re: [Histonet] Equipment selection >> >> If buying the Bond means that you are forced to "swallow" the Leica >> coverslipper go with Sakura and Dako. >> If they are making that "package" is because the Bond sells well but they >> are having to "swallow" the coverslipper. Price is important but in the >> long run it is better to increase productivity and avoid problems. The >> Sakura is much faster than the Leica coverslipper. >> Ren? J. >> >> >> ________________________________ >> From: "Huggins, Haley - MRMC" >> To: Rene J Buesa ; "histonet@lists.utsouthwestern.edu" >> >> Sent: Thursday, September 12, 2013 11:55 AM >> Subject: RE: [Histonet] Equipment selection >> >> >> >> I actually know all the equipment. Well, I know 3 of the pieces really >> well, the Leica Bond Max is new to me. I did go to another lab to see it as >> well as the Leica stainer/coverslipper. I was impressed with the Bond Max, >> not as much with the coverslipper part of the stainer/coverslipper. The big >> issue I am having is if I want to go with the Bond Max, I technically need >> to go with the Leica stainer/coverslipper because it is lumped into a big >> package deal. If I want the Sakura Prisma stainer/film coverslipper, I go >> with the Dako immunostainer. I am having trouble deciding. It is boiling >> down to money, which sucks since I might be forced to go with something >> that I might not be happy with in the future. >> >> Haley >> >> From:Rene J Buesa [mailto:rjbuesa@yahoo.com] >> Sent: Thursday, September 12, 2013 5:32 AM >> To: Huggins, Haley - MRMC; histonet@lists.utsouthwestern.edu >> Subject: Re: [Histonet] Equipment selection >> >> I used the Sakura and the Dako, but it seems that the Leica Bond Max is >> getting popularity. Why don't you go to a lab where they are in use and >> take a look? >> Ren?J. >> >> From:"Huggins, Haley - MRMC" >> To: "histonet@lists.utsouthwestern.edu" < >> histonet@lists.utsouthwestern.edu> >> Sent: Wednesday, September 11, 2013 4:46 PM >> Subject: [Histonet] Equipment selection >> >> Hi all, >> >> I am trying to decide on what equipment to purchase. I have picked the >> processor, microtomes, embedding centers and a few other pieces, but now I >> still need to decide on the H&E Stainer and the immunostainer. My options I >> have in front of me are for the H&E stainer are Sakura Prisma stainer/film >> coverslipper and the Leica ST5020-CV5030 stainer/glass coverslipper. For >> the Immunostainer, I am looking at the Dako Autostainer and the Leica Bond >> Max. Any advice on any of these instruments would be greatly appreciated. I >> am trying to finalize my decision pretty quickly. Thank you in advance for >> any input. >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> ---------------------------------------------------------------------- >> Confidentiality Notice: This e-mail message, including any attachments, >> is for the sole use of the intended recipient(s) and may contain >> confidential and privileged information. Any unauthorized review, use, >> disclosure or distribution is prohibited. If you are not the intended >> recipient, please contact the sender by reply e-mail and destroy all copies >> of the original message. >> >> >> >> ------------------------------ >> >> Message: 5 >> Date: Thu, 12 Sep 2013 11:45:41 -0500 >> From: "Webb, Dorothy L" >> Subject: [Histonet] Need your expertise >> To: "'histonet@lists.utsouthwestern.edu'" >> >> Message-ID: >> < >> 65365F35C0F2EF4D846EC3CA73E49C4302ACEFF2C823@HPEMX3.HealthPartners.int> >> >> Content-Type: text/plain; charset="us-ascii" >> >> We will finally be going green by recycling formalin. I would appreciate >> any advice or opinions on true recycling VS. a filtering type of system. As >> we all know, experience with a system or product is the best way to gain >> true knowledge! >> >> Also, I would appreciate knowing how others are handling extra levels of >> prostate needle bx's. We currently place them on charged slides and keep >> until the case is signed out, but it seems there should be a better way of >> handling this due to storage of the slides not to mention the extra cost >> involved! I know this is becoming more of a needed process with all of the >> RNA/DNA testing being done on diagnosed cancers. >> >> As always, I do appreciate any and all comments, advice, or words of >> wisdom!! Hope to see some of you in Providence late next week! >> >> Dorothy Webb, HT (ASCP) >> Regions Histology Technical Specialist >> 651-254-2962 >> >> >> >> ________________________________ >> This e-mail and any files transmitted with it are confidential and are >> intended solely for the use of the individual or entity to whom they are >> addressed. If you are not the intended recipient or the individual >> responsible for delivering the e-mail to the intended recipient, please be >> advised that you have received this e-mail in error and that any use, >> dissemination, forwarding, printing, or copying of this e-mail is strictly >> prohibited. >> >> If you have received this communication in error, please return it to the >> sender immediately and delete the original message and any copy of it from >> your computer system. If you have any questions concerning this message, >> please contact the sender. Disclaimer R001.0 >> >> >> ------------------------------ >> >> Message: 6 >> Date: Thu, 12 Sep 2013 11:55:43 -0500 >> From: Tim Higgins >> Subject: [Histonet] RE: Histonet Digest, Vol 118, Issue 23 >> To: "histonet@lists.utsouthwestern.edu" >> >> Message-ID: >> Content-Type: text/plain; charset="iso-8859-1" >> >> Hey Martha, >> >> You have to forgive Rene J. she speaks sometimes and has nothing good to >> say but keeps speaking. There is never a bad question, if you don't have a >> good answer then keep it to yourself as I usually do. >> >> "Adequate forum". really? I guess no one on Histonet is allowed to share >> their personal protocols, procedures, opinions, and experiences on here >> anymore. >> >> Come on Rene J., next time shhhhhhhhh!! >> >> I should have just hit the "Delete" button and follow my own advise! >> >> Tim N. Higgins, HT (ASCP), QIHC >> >> >> > Message: 7 >> > Date: Thu, 12 Sep 2013 06:22:34 -0700 (PDT) >> > From: Rene J Buesa >> > Subject: Re: [Histonet] Minimum sample size for breast markers >> > To: Martha Ward-Pathology , >> > "histonet@lists.utsouthwestern.edu" >> > >> > Message-ID: >> > <1378992154.20733.YahooMailNeo@web163104.mail.bf1.yahoo.com> >> > Content-Type: text/plain; charset=iso-8859-1 >> > >> > I do not think this is the adequate forum to ask this question because >> you may get a wrong answer that could determine your decision. >> > Your pathologist is the one who should decide about this issue. >> > Ren? J. >> > >> > >> > ________________________________ >> > From: Martha Ward-Pathology >> > To: "histonet@lists.utsouthwestern.edu" < >> histonet@lists.utsouthwestern.edu> >> > Sent: Thursday, September 12, 2013 9:06 AM >> > Subject: [Histonet] Minimum sample size for breast markers >> > >> > >> > >> > >> > Is there a minimum number of cells that should be present when testing >> for ER, PR and Her2? I have looked on the CAP website but cannot see >> anything about this. We are constantly getting these small biopsies or >> FNAs for testing and I sometimes wonder what the lowest threshold is for >> testing...20 tumor cells, 50 tumor cells???? Any information would be >> appreciated. Thanks in advance for your help. >> > >> > Martha Ward, MT (ASCP) QIHC >> > Manager >> > >> > Molecular Diagnostics Lab >> > Medical Center Boulevard \ Winston-Salem, NC 27157 >> > p 336.716.2109 \ f 336.716.5890 >> > mward@wakehealth.edu >> > >> >> >> ------------------------------ >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> End of Histonet Digest, Vol 118, Issue 24 >> ***************************************** >> > > From rsrichmond <@t> gmail.com Thu Sep 12 12:55:58 2013 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Thu Sep 12 12:56:01 2013 Subject: [Histonet] Re: Minimum sample size for breast markers Message-ID: Pathologists need to be very ready to note that a breast cancer sample size is small, and that the markers need to be repeated on the lumpectomy or mastectomy specimen. This is easier said than done. Popping a Dolly-size breast into too-small container and dribbling a little formalin on it is not going to preserve the specimen adequately. Even with a moderate-sized lumpectomy specimen the formaldehyde penetration is going to be chancy. This is an issue that surgeons, OR nurses, and too many pathologists are unable to understand. The pathologist has to arrange to receive the specimen promptly and get the tumor cut into formalin. Easier said than done. The woman's cancer care depends on this getting done. It's non-trivial. And colon cancer is distinctly the next frontier for this problem. Bob Richmond Samurai Pathologist Maryville TN From PAMarcum <@t> uams.edu Thu Sep 12 13:01:58 2013 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Thu Sep 12 13:02:08 2013 Subject: [Histonet] Re: Histonet Digest, Vole 118, Issue 24 Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA32D3158C08@Mail2Node2.ad.uams.edu> Note: Dako has a new Autostainer for IHC, IS, CASH etc. so it may wise to talk to them about that before making a decision. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Madeleine Huey Sent: Thursday, September 12, 2013 12:35 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Histonet Digest, Vol 118, Issue 24 O..o.oop! PC problem. Haley, I have experiences with all the above equipment's and here's my inf; 1) Leica H&E Autostainer + glass coverslipper - smaller size therefore use lesser counter space 2) Lesser cost $$ for Leica HE Autostainer / coverslipper 3) Bond Max/Bond III could do IHC & ISH simultaneously in one Autostainer, while DAKO IHC autostainer can do only IHC (no heating elements) 4) Bond can do DELAY start while you are sleeping & continuos flow as well, while DAKO need removed slides after AR & run immediately (batches) Madeleine Huey BS, HTL/QIHC (ASCP) Supervisor - IHC & Histology (Pathology) On Thu, Sep 12, 2013 at 10:18 AM, Madeleine Huey wrote: > From:"Huggins, Haley - MRMC" > To: "histonet@lists.utsouthwestern.edu" > > > Sent: Wednesday, September 11, 2013 4:46 PM > Subject: [Histonet] Equipment selection > > Hi all, > > I am trying to decide on what equipment to purchase. I have picked the > processor, microtomes, embedding centers and a few other pieces, but > now I still need to decide on the H&E Stainer and the immunostainer. > My options I have in front of me are for the H&E stainer are Sakura > Prisma stainer/film coverslipper and the Leica ST5020-CV5030 > stainer/glass coverslipper. For the Immunostainer, I am looking at the > Dako Autostainer and the Leica Bond Max. Any advice on any of these > instruments would be greatly appreciated. I am trying to finalize my > decision pretty quickly. Thank you in advance for any input. > > Haley, > I have experiences with all the above equipments and here's my inf; > 1) Leica H&E autostainer with glass coverslipper - smaller size (use > lesser counter space) cheaper (buy 2 equivalent to 2 Sakura Prisma + > film coverslipper and smaller. > > > On Thu, Sep 12, 2013 at 10:02 AM, < > histonet-request@lists.utsouthwestern.edu> wrote: > >> Send Histonet mailing list submissions to >> histonet@lists.utsouthwestern.edu >> >> To subscribe or unsubscribe via the World Wide Web, visit >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> or, via email, send a message with subject or body 'help' to >> histonet-request@lists.utsouthwestern.edu >> >> You can reach the person managing the list at >> histonet-owner@lists.utsouthwestern.edu >> >> When replying, please edit your Subject line so it is more specific >> than "Re: Contents of Histonet digest..." >> >> >> Today's Topics: >> >> 1. RE: Equipment selection (Huggins, Haley - MRMC) >> 2. Re: Equipment selection (Rene J Buesa) >> 3. RE: Equipment selection (Huggins, Haley - MRMC) >> 4. RE: Equipment selection (Marcum, Pamela A) >> 5. Need your expertise (Webb, Dorothy L) >> 6. RE: Histonet Digest, Vol 118, Issue 23 (Tim Higgins) >> >> >> --------------------------------------------------------------------- >> - >> >> Message: 1 >> Date: Thu, 12 Sep 2013 08:55:39 -0700 >> From: "Huggins, Haley - MRMC" >> Subject: RE: [Histonet] Equipment selection >> To: Rene J Buesa , >> "histonet@lists.utsouthwestern.edu" >> >> Message-ID: >> < >> 4F36EC93A5737D4F8A2974E8FB8E260615F5698C69@PHX-MSG-007-N2.chw.edu> >> Content-Type: text/plain; charset="iso-8859-1" >> >> I actually know all the equipment. Well, I know 3 of the pieces >> really well, the Leica Bond Max is new to me. I did go to another lab >> to see it as well as the Leica stainer/coverslipper. I was impressed >> with the Bond Max, not as much with the coverslipper part of the >> stainer/coverslipper. The big issue I am having is if I want to go >> with the Bond Max, I technically need to go with the Leica >> stainer/coverslipper because it is lumped into a big package deal. If >> I want the Sakura Prisma stainer/film coverslipper, I go with the >> Dako immunostainer. I am having trouble deciding. It is boiling down >> to money, which sucks since I might be forced to go with something that I might not be happy with in the future. >> >> Haley >> >> From: Rene J Buesa [mailto:rjbuesa@yahoo.com] >> Sent: Thursday, September 12, 2013 5:32 AM >> To: Huggins, Haley - MRMC; histonet@lists.utsouthwestern.edu >> Subject: Re: [Histonet] Equipment selection >> >> I used the Sakura and the Dako, but it seems that the Leica Bond Max >> is getting popularity. Why don't you go to a lab where they are in >> use and take a look? >> Ren? J. >> >> From: "Huggins, Haley - MRMC" > Haley.Huggins@DignityHealth.org>> >> To: "histonet@lists.utsouthwestern.edu> histonet@lists.utsouthwestern.edu>" >> > > >> Sent: Wednesday, September 11, 2013 4:46 PM >> Subject: [Histonet] Equipment selection >> >> Hi all, >> >> I am trying to decide on what equipment to purchase. I have picked >> the processor, microtomes, embedding centers and a few other pieces, >> but now I still need to decide on the H&E Stainer and the >> immunostainer. My options I have in front of me are for the H&E >> stainer are Sakura Prisma stainer/film coverslipper and the Leica >> ST5020-CV5030 stainer/glass coverslipper. For the Immunostainer, I am >> looking at the Dako Autostainer and the Leica Bond Max. Any advice on >> any of these instruments would be greatly appreciated. I am trying to >> finalize my decision pretty quickly. Thank you in advance for any input. >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu> Histonet@lists.utsouthwestern.edu> >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> ------------------------------ >> >> Message: 2 >> Date: Thu, 12 Sep 2013 09:07:56 -0700 (PDT) >> From: Rene J Buesa >> Subject: Re: [Histonet] Equipment selection >> To: "Huggins, Haley - MRMC" , >> "histonet@lists.utsouthwestern.edu" >> >> Message-ID: >> <1379002076.53612.YahooMailNeo@web163105.mail.bf1.yahoo.com> >> Content-Type: text/plain; charset=iso-8859-1 >> >> If buying the Bond means that you are forced to "swallow" the Leica >> coverslipper go with Sakura and Dako. >> If they are making that "package" is because the Bond sells well but >> they are having to "swallow" the coverslipper. Price is important but >> in the long run it is better to increase productivity and avoid >> problems. The Sakura is much faster than the Leica coverslipper. >> Ren? J. >> >> >> ________________________________ >> From: "Huggins, Haley - MRMC" >> To: Rene J Buesa ; "histonet@lists.utsouthwestern.edu" >> >> Sent: Thursday, September 12, 2013 11:55 AM >> Subject: RE: [Histonet] Equipment selection >> >> >> >> I actually know all the equipment. Well, I know 3 of the pieces >> really well, the Leica Bond Max is new to me. I did go to another lab >> to see it as well as the Leica stainer/coverslipper. I was impressed >> with the Bond Max, not as much with the coverslipper part of the >> stainer/coverslipper. The big issue I am having is if I want to go >> with the Bond Max, I technically need to go with the Leica >> stainer/coverslipper because it is lumped into a big package deal. If >> I want the Sakura Prisma stainer/film coverslipper, I go with the >> Dako immunostainer. I am having trouble deciding. It is boiling down >> to money, which sucks since I might be forced to go with something that I might not be happy with in the future. >> >> Haley >> >> From:Rene J Buesa [mailto:rjbuesa@yahoo.com] >> Sent: Thursday, September 12, 2013 5:32 AM >> To: Huggins, Haley - MRMC; histonet@lists.utsouthwestern.edu >> Subject: Re: [Histonet] Equipment selection >> >> I used the Sakura and the Dako, but it seems that the Leica Bond Max >> is getting popularity. Why don't you go to a lab where they are in >> use and take a look? >> Ren?J. >> >> From:"Huggins, Haley - MRMC" >> To: "histonet@lists.utsouthwestern.edu" < >> histonet@lists.utsouthwestern.edu> >> Sent: Wednesday, September 11, 2013 4:46 PM >> Subject: [Histonet] Equipment selection >> >> Hi all, >> >> I am trying to decide on what equipment to purchase. I have picked >> the processor, microtomes, embedding centers and a few other pieces, >> but now I still need to decide on the H&E Stainer and the >> immunostainer. My options I have in front of me are for the H&E >> stainer are Sakura Prisma stainer/film coverslipper and the Leica >> ST5020-CV5030 stainer/glass coverslipper. For the Immunostainer, I am >> looking at the Dako Autostainer and the Leica Bond Max. Any advice on >> any of these instruments would be greatly appreciated. I am trying to >> finalize my decision pretty quickly. Thank you in advance for any input. >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> ------------------------------ >> >> Message: 3 >> Date: Thu, 12 Sep 2013 09:10:47 -0700 >> From: "Huggins, Haley - MRMC" >> Subject: RE: [Histonet] Equipment selection >> To: Rene J Buesa , >> "histonet@lists.utsouthwestern.edu" >> >> Message-ID: >> < >> 4F36EC93A5737D4F8A2974E8FB8E260615F5698C6D@PHX-MSG-007-N2.chw.edu> >> Content-Type: text/plain; charset="iso-8859-1" >> >> Thanks, that is a good point. >> >> From: Rene J Buesa [mailto:rjbuesa@yahoo.com] >> Sent: Thursday, September 12, 2013 9:08 AM >> To: Huggins, Haley - MRMC; histonet@lists.utsouthwestern.edu >> Subject: Re: [Histonet] Equipment selection >> >> If buying the Bond means that you are forced to "swallow" the Leica >> coverslipper go with Sakura and Dako. >> If they are making that "package" is because the Bond sells well but >> they are having to "swallow" the coverslipper. Price is important but >> in the long run it is better to increase productivity and avoid >> problems. The Sakura is much faster than the Leica coverslipper. >> Ren? J. >> >> From: "Huggins, Haley - MRMC" > Haley.Huggins@DignityHealth.org>> >> To: Rene J Buesa >; " >> histonet@lists.utsouthwestern.edu> histonet@lists.utsouthwestern.edu>" >> > > >> Sent: Thursday, September 12, 2013 11:55 AM >> Subject: RE: [Histonet] Equipment selection >> >> I actually know all the equipment. Well, I know 3 of the pieces >> really well, the Leica Bond Max is new to me. I did go to another lab >> to see it as well as the Leica stainer/coverslipper. I was impressed >> with the Bond Max, not as much with the coverslipper part of the >> stainer/coverslipper. The big issue I am having is if I want to go >> with the Bond Max, I technically need to go with the Leica >> stainer/coverslipper because it is lumped into a big package deal. If >> I want the Sakura Prisma stainer/film coverslipper, I go with the >> Dako immunostainer. I am having trouble deciding. It is boiling down >> to money, which sucks since I might be forced to go with something that I might not be happy with in the future. >> >> Haley >> >> From: Rene J Buesa [mailto:rjbuesa@yahoo.com] >> Sent: Thursday, September 12, 2013 5:32 AM >> To: Huggins, Haley - MRMC; histonet@lists.utsouthwestern.edu> histonet@lists.utsouthwestern.edu> >> Subject: Re: [Histonet] Equipment selection >> >> I used the Sakura and the Dako, but it seems that the Leica Bond Max >> is getting popularity. Why don't you go to a lab where they are in >> use and take a look? >> Ren? J. >> >> From: "Huggins, Haley - MRMC" > Haley.Huggins@DignityHealth.org>> >> To: "histonet@lists.utsouthwestern.edu> histonet@lists.utsouthwestern.edu>" >> > > >> Sent: Wednesday, September 11, 2013 4:46 PM >> Subject: [Histonet] Equipment selection >> >> Hi all, >> >> I am trying to decide on what equipment to purchase. I have picked >> the processor, microtomes, embedding centers and a few other pieces, >> but now I still need to decide on the H&E Stainer and the >> immunostainer. My options I have in front of me are for the H&E >> stainer are Sakura Prisma stainer/film coverslipper and the Leica >> ST5020-CV5030 stainer/glass coverslipper. For the Immunostainer, I am >> looking at the Dako Autostainer and the Leica Bond Max. Any advice on >> any of these instruments would be greatly appreciated. I am trying to >> finalize my decision pretty quickly. Thank you in advance for any input. >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu> Histonet@lists.utsouthwestern.edu> >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> ------------------------------ >> >> Message: 4 >> Date: Thu, 12 Sep 2013 16:30:13 +0000 >> From: "Marcum, Pamela A" >> Subject: RE: [Histonet] Equipment selection >> To: "'Rene J Buesa'" , "Huggins, Haley - MRMC" >> , " >> histonet@lists.utsouthwestern.edu" >> >> Message-ID: >> >> <41D3A1AF6FEF0643BDC89E0516A6EA32D3158B57@Mail2Node2.ad.uams.edu> >> Content-Type: text/plain; charset="iso-8859-1" >> >> Actually the coverslipper from Leica is very good and can be linked >> to the stainer. It is also a glass coverslipper not tape. It is >> always about money and that may be the thing that decides no matter what any of prefers. >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu [mailto: >> histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa >> Sent: Thursday, September 12, 2013 11:08 AM >> To: Huggins, Haley - MRMC; histonet@lists.utsouthwestern.edu >> Subject: Re: [Histonet] Equipment selection >> >> If buying the Bond means that you are forced to "swallow" the Leica >> coverslipper go with Sakura and Dako. >> If they are making that "package" is because the Bond sells well but >> they are having to "swallow" the coverslipper. Price is important but >> in the long run it is better to increase productivity and avoid >> problems. The Sakura is much faster than the Leica coverslipper. >> Ren? J. >> >> >> ________________________________ >> From: "Huggins, Haley - MRMC" >> To: Rene J Buesa ; "histonet@lists.utsouthwestern.edu" >> >> Sent: Thursday, September 12, 2013 11:55 AM >> Subject: RE: [Histonet] Equipment selection >> >> >> >> I actually know all the equipment. Well, I know 3 of the pieces >> really well, the Leica Bond Max is new to me. I did go to another lab >> to see it as well as the Leica stainer/coverslipper. I was impressed >> with the Bond Max, not as much with the coverslipper part of the >> stainer/coverslipper. The big issue I am having is if I want to go >> with the Bond Max, I technically need to go with the Leica >> stainer/coverslipper because it is lumped into a big package deal. If >> I want the Sakura Prisma stainer/film coverslipper, I go with the >> Dako immunostainer. I am having trouble deciding. It is boiling down >> to money, which sucks since I might be forced to go with something that I might not be happy with in the future. >> >> Haley >> >> From:Rene J Buesa [mailto:rjbuesa@yahoo.com] >> Sent: Thursday, September 12, 2013 5:32 AM >> To: Huggins, Haley - MRMC; histonet@lists.utsouthwestern.edu >> Subject: Re: [Histonet] Equipment selection >> >> I used the Sakura and the Dako, but it seems that the Leica Bond Max >> is getting popularity. Why don't you go to a lab where they are in >> use and take a look? >> Ren?J. >> >> From:"Huggins, Haley - MRMC" >> To: "histonet@lists.utsouthwestern.edu" < >> histonet@lists.utsouthwestern.edu> >> Sent: Wednesday, September 11, 2013 4:46 PM >> Subject: [Histonet] Equipment selection >> >> Hi all, >> >> I am trying to decide on what equipment to purchase. I have picked >> the processor, microtomes, embedding centers and a few other pieces, >> but now I still need to decide on the H&E Stainer and the >> immunostainer. My options I have in front of me are for the H&E >> stainer are Sakura Prisma stainer/film coverslipper and the Leica >> ST5020-CV5030 stainer/glass coverslipper. For the Immunostainer, I am >> looking at the Dako Autostainer and the Leica Bond Max. Any advice on >> any of these instruments would be greatly appreciated. I am trying to >> finalize my decision pretty quickly. Thank you in advance for any input. >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> --------------------------------------------------------------------- >> - Confidentiality Notice: This e-mail message, including any >> attachments, is for the sole use of the intended recipient(s) and may >> contain confidential and privileged information. Any unauthorized >> review, use, disclosure or distribution is prohibited. If you are not >> the intended recipient, please contact the sender by reply e-mail and >> destroy all copies of the original message. >> >> >> >> ------------------------------ >> >> Message: 5 >> Date: Thu, 12 Sep 2013 11:45:41 -0500 >> From: "Webb, Dorothy L" >> Subject: [Histonet] Need your expertise >> To: "'histonet@lists.utsouthwestern.edu'" >> >> Message-ID: >> < >> 65365F35C0F2EF4D846EC3CA73E49C4302ACEFF2C823@HPEMX3.HealthPartners.in >> t> >> >> Content-Type: text/plain; charset="us-ascii" >> >> We will finally be going green by recycling formalin. I would >> appreciate any advice or opinions on true recycling VS. a filtering >> type of system. As we all know, experience with a system or product >> is the best way to gain true knowledge! >> >> Also, I would appreciate knowing how others are handling extra levels >> of prostate needle bx's. We currently place them on charged slides >> and keep until the case is signed out, but it seems there should be a >> better way of handling this due to storage of the slides not to >> mention the extra cost involved! I know this is becoming more of a >> needed process with all of the RNA/DNA testing being done on diagnosed cancers. >> >> As always, I do appreciate any and all comments, advice, or words of >> wisdom!! Hope to see some of you in Providence late next week! >> >> Dorothy Webb, HT (ASCP) >> Regions Histology Technical Specialist >> 651-254-2962 >> >> >> >> ________________________________ >> This e-mail and any files transmitted with it are confidential and >> are intended solely for the use of the individual or entity to whom >> they are addressed. If you are not the intended recipient or the >> individual responsible for delivering the e-mail to the intended >> recipient, please be advised that you have received this e-mail in >> error and that any use, dissemination, forwarding, printing, or >> copying of this e-mail is strictly prohibited. >> >> If you have received this communication in error, please return it to >> the sender immediately and delete the original message and any copy >> of it from your computer system. If you have any questions concerning >> this message, please contact the sender. Disclaimer R001.0 >> >> >> ------------------------------ >> >> Message: 6 >> Date: Thu, 12 Sep 2013 11:55:43 -0500 >> From: Tim Higgins >> Subject: [Histonet] RE: Histonet Digest, Vol 118, Issue 23 >> To: "histonet@lists.utsouthwestern.edu" >> >> Message-ID: >> Content-Type: text/plain; charset="iso-8859-1" >> >> Hey Martha, >> >> You have to forgive Rene J. she speaks sometimes and has nothing good >> to say but keeps speaking. There is never a bad question, if you >> don't have a good answer then keep it to yourself as I usually do. >> >> "Adequate forum". really? I guess no one on Histonet is allowed to >> share their personal protocols, procedures, opinions, and experiences >> on here anymore. >> >> Come on Rene J., next time shhhhhhhhh!! >> >> I should have just hit the "Delete" button and follow my own advise! >> >> Tim N. Higgins, HT (ASCP), QIHC >> >> >> > Message: 7 >> > Date: Thu, 12 Sep 2013 06:22:34 -0700 (PDT) >> > From: Rene J Buesa >> > Subject: Re: [Histonet] Minimum sample size for breast markers >> > To: Martha Ward-Pathology , >> > "histonet@lists.utsouthwestern.edu" >> > >> > Message-ID: >> > <1378992154.20733.YahooMailNeo@web163104.mail.bf1.yahoo.com> >> > Content-Type: text/plain; charset=iso-8859-1 >> > >> > I do not think this is the adequate forum to ask this question >> > because >> you may get a wrong answer that could determine your decision. >> > Your pathologist is the one who should decide about this issue. >> > Ren? J. >> > >> > >> > ________________________________ >> > From: Martha Ward-Pathology >> > To: "histonet@lists.utsouthwestern.edu" < >> histonet@lists.utsouthwestern.edu> >> > Sent: Thursday, September 12, 2013 9:06 AM >> > Subject: [Histonet] Minimum sample size for breast markers >> > >> > >> > >> > >> > Is there a minimum number of cells that should be present when >> > testing >> for ER, PR and Her2? I have looked on the CAP website but cannot see >> anything about this. We are constantly getting these small biopsies >> or FNAs for testing and I sometimes wonder what the lowest threshold >> is for >> testing...20 tumor cells, 50 tumor cells???? Any information would >> be appreciated. Thanks in advance for your help. >> > >> > Martha Ward, MT (ASCP) QIHC >> > Manager >> > >> > Molecular Diagnostics Lab >> > Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 >> > \ f 336.716.5890 mward@wakehealth.edu >> > >> >> >> ------------------------------ >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> End of Histonet Digest, Vol 118, Issue 24 >> ***************************************** >> > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From pev <@t> xs4all.nl Thu Sep 12 13:03:22 2013 From: pev <@t> xs4all.nl (P.E. Visser) Date: Thu Sep 12 13:03:08 2013 Subject: [Histonet] Equipment selection Message-ID: <000801ceafe2$5fbdff80$1f39fe80$@xs4all.nl> Hi Maybe you know but sakura also offers a glas coverslipper in combination with their prisma stainer. i have worked with both strainers and definitely would select the prisma. the Leica system splashes (or drops) solutions on its way from bath to bath. look at an older system and you will see stain drops where you don't expect them. also there water bathes are assigned per position to a stain that is way less flexible then the sakura system where the watherbathes form a groop and the stainer selects which one is free. in our case we limmeted the amount of different stains in both machines to allow higher HE trughput. I wil gladly supply you with more information. Piet visser ----------------------------------------- Hi all, I am trying to decide on what equipment to purchase. I have picked the processor, microtomes, embedding centers and a few other pieces, but now I still need to decide on the H&E Stainer and the immunostainer. My options I have in front of me are for the H&E stainer are Sakura Prisma stainer/film coverslipper and the Leica ST5020-CV5030 stainer/glass coverslipper. For the Immunostainer, I am looking at the Dako Autostainer and the Leica Bond Max. Any advice on any of these instruments would be greatly appreciated. I am trying to finalize my decision pretty quickly. Thank you in advance for any input. From PAMarcum <@t> uams.edu Thu Sep 12 13:03:43 2013 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Thu Sep 12 13:03:49 2013 Subject: [Histonet] Re: Histonet Digest, Vol 118, Issue 24 In-Reply-To: References: <5231f38d.e9363c0a.4773.ffffb744SMTPIN_ADDED_MISSING@mx.google.com> Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA32D3158C1D@Mail2Node2.ad.uams.edu> Sorry I think that went out with me messing up spell check. Hit the button too fast. Anyway I am sure the point is there Dako - new - unit - better ability for IHC, ISH, CISH etc. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Madeleine Huey Sent: Thursday, September 12, 2013 12:35 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Histonet Digest, Vol 118, Issue 24 O..o.oop! PC problem. Haley, I have experiences with all the above equipment's and here's my inf; 1) Leica H&E Autostainer + glass coverslipper - smaller size therefore use lesser counter space 2) Lesser cost $$ for Leica HE Autostainer / coverslipper 3) Bond Max/Bond III could do IHC & ISH simultaneously in one Autostainer, while DAKO IHC autostainer can do only IHC (no heating elements) 4) Bond can do DELAY start while you are sleeping & continuos flow as well, while DAKO need removed slides after AR & run immediately (batches) Madeleine Huey BS, HTL/QIHC (ASCP) Supervisor - IHC & Histology (Pathology) On Thu, Sep 12, 2013 at 10:18 AM, Madeleine Huey wrote: > From:"Huggins, Haley - MRMC" > To: "histonet@lists.utsouthwestern.edu" > > > Sent: Wednesday, September 11, 2013 4:46 PM > Subject: [Histonet] Equipment selection > > Hi all, > > I am trying to decide on what equipment to purchase. I have picked the > processor, microtomes, embedding centers and a few other pieces, but > now I still need to decide on the H&E Stainer and the immunostainer. > My options I have in front of me are for the H&E stainer are Sakura > Prisma stainer/film coverslipper and the Leica ST5020-CV5030 > stainer/glass coverslipper. For the Immunostainer, I am looking at the > Dako Autostainer and the Leica Bond Max. Any advice on any of these > instruments would be greatly appreciated. I am trying to finalize my > decision pretty quickly. Thank you in advance for any input. > > Haley, > I have experiences with all the above equipments and here's my inf; > 1) Leica H&E autostainer with glass coverslipper - smaller size (use > lesser counter space) cheaper (buy 2 equivalent to 2 Sakura Prisma + > film coverslipper and smaller. > > > On Thu, Sep 12, 2013 at 10:02 AM, < > histonet-request@lists.utsouthwestern.edu> wrote: > >> Send Histonet mailing list submissions to >> histonet@lists.utsouthwestern.edu >> >> To subscribe or unsubscribe via the World Wide Web, visit >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> or, via email, send a message with subject or body 'help' to >> histonet-request@lists.utsouthwestern.edu >> >> You can reach the person managing the list at >> histonet-owner@lists.utsouthwestern.edu >> >> When replying, please edit your Subject line so it is more specific >> than "Re: Contents of Histonet digest..." >> >> >> Today's Topics: >> >> 1. RE: Equipment selection (Huggins, Haley - MRMC) >> 2. Re: Equipment selection (Rene J Buesa) >> 3. RE: Equipment selection (Huggins, Haley - MRMC) >> 4. RE: Equipment selection (Marcum, Pamela A) >> 5. Need your expertise (Webb, Dorothy L) >> 6. RE: Histonet Digest, Vol 118, Issue 23 (Tim Higgins) >> >> >> --------------------------------------------------------------------- >> - >> >> Message: 1 >> Date: Thu, 12 Sep 2013 08:55:39 -0700 >> From: "Huggins, Haley - MRMC" >> Subject: RE: [Histonet] Equipment selection >> To: Rene J Buesa , >> "histonet@lists.utsouthwestern.edu" >> >> Message-ID: >> < >> 4F36EC93A5737D4F8A2974E8FB8E260615F5698C69@PHX-MSG-007-N2.chw.edu> >> Content-Type: text/plain; charset="iso-8859-1" >> >> I actually know all the equipment. Well, I know 3 of the pieces >> really well, the Leica Bond Max is new to me. I did go to another lab >> to see it as well as the Leica stainer/coverslipper. I was impressed >> with the Bond Max, not as much with the coverslipper part of the >> stainer/coverslipper. The big issue I am having is if I want to go >> with the Bond Max, I technically need to go with the Leica >> stainer/coverslipper because it is lumped into a big package deal. If >> I want the Sakura Prisma stainer/film coverslipper, I go with the >> Dako immunostainer. I am having trouble deciding. It is boiling down >> to money, which sucks since I might be forced to go with something that I might not be happy with in the future. >> >> Haley >> >> From: Rene J Buesa [mailto:rjbuesa@yahoo.com] >> Sent: Thursday, September 12, 2013 5:32 AM >> To: Huggins, Haley - MRMC; histonet@lists.utsouthwestern.edu >> Subject: Re: [Histonet] Equipment selection >> >> I used the Sakura and the Dako, but it seems that the Leica Bond Max >> is getting popularity. Why don't you go to a lab where they are in >> use and take a look? >> Ren? J. >> >> From: "Huggins, Haley - MRMC" > Haley.Huggins@DignityHealth.org>> >> To: "histonet@lists.utsouthwestern.edu> histonet@lists.utsouthwestern.edu>" >> > > >> Sent: Wednesday, September 11, 2013 4:46 PM >> Subject: [Histonet] Equipment selection >> >> Hi all, >> >> I am trying to decide on what equipment to purchase. I have picked >> the processor, microtomes, embedding centers and a few other pieces, >> but now I still need to decide on the H&E Stainer and the >> immunostainer. My options I have in front of me are for the H&E >> stainer are Sakura Prisma stainer/film coverslipper and the Leica >> ST5020-CV5030 stainer/glass coverslipper. For the Immunostainer, I am >> looking at the Dako Autostainer and the Leica Bond Max. Any advice on >> any of these instruments would be greatly appreciated. I am trying to >> finalize my decision pretty quickly. Thank you in advance for any input. >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu> Histonet@lists.utsouthwestern.edu> >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> ------------------------------ >> >> Message: 2 >> Date: Thu, 12 Sep 2013 09:07:56 -0700 (PDT) >> From: Rene J Buesa >> Subject: Re: [Histonet] Equipment selection >> To: "Huggins, Haley - MRMC" , >> "histonet@lists.utsouthwestern.edu" >> >> Message-ID: >> <1379002076.53612.YahooMailNeo@web163105.mail.bf1.yahoo.com> >> Content-Type: text/plain; charset=iso-8859-1 >> >> If buying the Bond means that you are forced to "swallow" the Leica >> coverslipper go with Sakura and Dako. >> If they are making that "package" is because the Bond sells well but >> they are having to "swallow" the coverslipper. Price is important but >> in the long run it is better to increase productivity and avoid >> problems. The Sakura is much faster than the Leica coverslipper. >> Ren? J. >> >> >> ________________________________ >> From: "Huggins, Haley - MRMC" >> To: Rene J Buesa ; "histonet@lists.utsouthwestern.edu" >> >> Sent: Thursday, September 12, 2013 11:55 AM >> Subject: RE: [Histonet] Equipment selection >> >> >> >> I actually know all the equipment. Well, I know 3 of the pieces >> really well, the Leica Bond Max is new to me. I did go to another lab >> to see it as well as the Leica stainer/coverslipper. I was impressed >> with the Bond Max, not as much with the coverslipper part of the >> stainer/coverslipper. The big issue I am having is if I want to go >> with the Bond Max, I technically need to go with the Leica >> stainer/coverslipper because it is lumped into a big package deal. If >> I want the Sakura Prisma stainer/film coverslipper, I go with the >> Dako immunostainer. I am having trouble deciding. It is boiling down >> to money, which sucks since I might be forced to go with something that I might not be happy with in the future. >> >> Haley >> >> From:Rene J Buesa [mailto:rjbuesa@yahoo.com] >> Sent: Thursday, September 12, 2013 5:32 AM >> To: Huggins, Haley - MRMC; histonet@lists.utsouthwestern.edu >> Subject: Re: [Histonet] Equipment selection >> >> I used the Sakura and the Dako, but it seems that the Leica Bond Max >> is getting popularity. Why don't you go to a lab where they are in >> use and take a look? >> Ren?J. >> >> From:"Huggins, Haley - MRMC" >> To: "histonet@lists.utsouthwestern.edu" < >> histonet@lists.utsouthwestern.edu> >> Sent: Wednesday, September 11, 2013 4:46 PM >> Subject: [Histonet] Equipment selection >> >> Hi all, >> >> I am trying to decide on what equipment to purchase. I have picked >> the processor, microtomes, embedding centers and a few other pieces, >> but now I still need to decide on the H&E Stainer and the >> immunostainer. My options I have in front of me are for the H&E >> stainer are Sakura Prisma stainer/film coverslipper and the Leica >> ST5020-CV5030 stainer/glass coverslipper. For the Immunostainer, I am >> looking at the Dako Autostainer and the Leica Bond Max. Any advice on >> any of these instruments would be greatly appreciated. I am trying to >> finalize my decision pretty quickly. Thank you in advance for any input. >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> ------------------------------ >> >> Message: 3 >> Date: Thu, 12 Sep 2013 09:10:47 -0700 >> From: "Huggins, Haley - MRMC" >> Subject: RE: [Histonet] Equipment selection >> To: Rene J Buesa , >> "histonet@lists.utsouthwestern.edu" >> >> Message-ID: >> < >> 4F36EC93A5737D4F8A2974E8FB8E260615F5698C6D@PHX-MSG-007-N2.chw.edu> >> Content-Type: text/plain; charset="iso-8859-1" >> >> Thanks, that is a good point. >> >> From: Rene J Buesa [mailto:rjbuesa@yahoo.com] >> Sent: Thursday, September 12, 2013 9:08 AM >> To: Huggins, Haley - MRMC; histonet@lists.utsouthwestern.edu >> Subject: Re: [Histonet] Equipment selection >> >> If buying the Bond means that you are forced to "swallow" the Leica >> coverslipper go with Sakura and Dako. >> If they are making that "package" is because the Bond sells well but >> they are having to "swallow" the coverslipper. Price is important but >> in the long run it is better to increase productivity and avoid >> problems. The Sakura is much faster than the Leica coverslipper. >> Ren? J. >> >> From: "Huggins, Haley - MRMC" > Haley.Huggins@DignityHealth.org>> >> To: Rene J Buesa >; " >> histonet@lists.utsouthwestern.edu> histonet@lists.utsouthwestern.edu>" >> > > >> Sent: Thursday, September 12, 2013 11:55 AM >> Subject: RE: [Histonet] Equipment selection >> >> I actually know all the equipment. Well, I know 3 of the pieces >> really well, the Leica Bond Max is new to me. I did go to another lab >> to see it as well as the Leica stainer/coverslipper. I was impressed >> with the Bond Max, not as much with the coverslipper part of the >> stainer/coverslipper. The big issue I am having is if I want to go >> with the Bond Max, I technically need to go with the Leica >> stainer/coverslipper because it is lumped into a big package deal. If >> I want the Sakura Prisma stainer/film coverslipper, I go with the >> Dako immunostainer. I am having trouble deciding. It is boiling down >> to money, which sucks since I might be forced to go with something that I might not be happy with in the future. >> >> Haley >> >> From: Rene J Buesa [mailto:rjbuesa@yahoo.com] >> Sent: Thursday, September 12, 2013 5:32 AM >> To: Huggins, Haley - MRMC; histonet@lists.utsouthwestern.edu> histonet@lists.utsouthwestern.edu> >> Subject: Re: [Histonet] Equipment selection >> >> I used the Sakura and the Dako, but it seems that the Leica Bond Max >> is getting popularity. Why don't you go to a lab where they are in >> use and take a look? >> Ren? J. >> >> From: "Huggins, Haley - MRMC" > Haley.Huggins@DignityHealth.org>> >> To: "histonet@lists.utsouthwestern.edu> histonet@lists.utsouthwestern.edu>" >> > > >> Sent: Wednesday, September 11, 2013 4:46 PM >> Subject: [Histonet] Equipment selection >> >> Hi all, >> >> I am trying to decide on what equipment to purchase. I have picked >> the processor, microtomes, embedding centers and a few other pieces, >> but now I still need to decide on the H&E Stainer and the >> immunostainer. My options I have in front of me are for the H&E >> stainer are Sakura Prisma stainer/film coverslipper and the Leica >> ST5020-CV5030 stainer/glass coverslipper. For the Immunostainer, I am >> looking at the Dako Autostainer and the Leica Bond Max. Any advice on >> any of these instruments would be greatly appreciated. I am trying to >> finalize my decision pretty quickly. Thank you in advance for any input. >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu> Histonet@lists.utsouthwestern.edu> >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> ------------------------------ >> >> Message: 4 >> Date: Thu, 12 Sep 2013 16:30:13 +0000 >> From: "Marcum, Pamela A" >> Subject: RE: [Histonet] Equipment selection >> To: "'Rene J Buesa'" , "Huggins, Haley - MRMC" >> , " >> histonet@lists.utsouthwestern.edu" >> >> Message-ID: >> >> <41D3A1AF6FEF0643BDC89E0516A6EA32D3158B57@Mail2Node2.ad.uams.edu> >> Content-Type: text/plain; charset="iso-8859-1" >> >> Actually the coverslipper from Leica is very good and can be linked >> to the stainer. It is also a glass coverslipper not tape. It is >> always about money and that may be the thing that decides no matter what any of prefers. >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu [mailto: >> histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa >> Sent: Thursday, September 12, 2013 11:08 AM >> To: Huggins, Haley - MRMC; histonet@lists.utsouthwestern.edu >> Subject: Re: [Histonet] Equipment selection >> >> If buying the Bond means that you are forced to "swallow" the Leica >> coverslipper go with Sakura and Dako. >> If they are making that "package" is because the Bond sells well but >> they are having to "swallow" the coverslipper. Price is important but >> in the long run it is better to increase productivity and avoid >> problems. The Sakura is much faster than the Leica coverslipper. >> Ren? J. >> >> >> ________________________________ >> From: "Huggins, Haley - MRMC" >> To: Rene J Buesa ; "histonet@lists.utsouthwestern.edu" >> >> Sent: Thursday, September 12, 2013 11:55 AM >> Subject: RE: [Histonet] Equipment selection >> >> >> >> I actually know all the equipment. Well, I know 3 of the pieces >> really well, the Leica Bond Max is new to me. I did go to another lab >> to see it as well as the Leica stainer/coverslipper. I was impressed >> with the Bond Max, not as much with the coverslipper part of the >> stainer/coverslipper. The big issue I am having is if I want to go >> with the Bond Max, I technically need to go with the Leica >> stainer/coverslipper because it is lumped into a big package deal. If >> I want the Sakura Prisma stainer/film coverslipper, I go with the >> Dako immunostainer. I am having trouble deciding. It is boiling down >> to money, which sucks since I might be forced to go with something that I might not be happy with in the future. >> >> Haley >> >> From:Rene J Buesa [mailto:rjbuesa@yahoo.com] >> Sent: Thursday, September 12, 2013 5:32 AM >> To: Huggins, Haley - MRMC; histonet@lists.utsouthwestern.edu >> Subject: Re: [Histonet] Equipment selection >> >> I used the Sakura and the Dako, but it seems that the Leica Bond Max >> is getting popularity. Why don't you go to a lab where they are in >> use and take a look? >> Ren?J. >> >> From:"Huggins, Haley - MRMC" >> To: "histonet@lists.utsouthwestern.edu" < >> histonet@lists.utsouthwestern.edu> >> Sent: Wednesday, September 11, 2013 4:46 PM >> Subject: [Histonet] Equipment selection >> >> Hi all, >> >> I am trying to decide on what equipment to purchase. I have picked >> the processor, microtomes, embedding centers and a few other pieces, >> but now I still need to decide on the H&E Stainer and the >> immunostainer. My options I have in front of me are for the H&E >> stainer are Sakura Prisma stainer/film coverslipper and the Leica >> ST5020-CV5030 stainer/glass coverslipper. For the Immunostainer, I am >> looking at the Dako Autostainer and the Leica Bond Max. Any advice on >> any of these instruments would be greatly appreciated. I am trying to >> finalize my decision pretty quickly. Thank you in advance for any input. >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> --------------------------------------------------------------------- >> - Confidentiality Notice: This e-mail message, including any >> attachments, is for the sole use of the intended recipient(s) and may >> contain confidential and privileged information. Any unauthorized >> review, use, disclosure or distribution is prohibited. If you are not >> the intended recipient, please contact the sender by reply e-mail and >> destroy all copies of the original message. >> >> >> >> ------------------------------ >> >> Message: 5 >> Date: Thu, 12 Sep 2013 11:45:41 -0500 >> From: "Webb, Dorothy L" >> Subject: [Histonet] Need your expertise >> To: "'histonet@lists.utsouthwestern.edu'" >> >> Message-ID: >> < >> 65365F35C0F2EF4D846EC3CA73E49C4302ACEFF2C823@HPEMX3.HealthPartners.in >> t> >> >> Content-Type: text/plain; charset="us-ascii" >> >> We will finally be going green by recycling formalin. I would >> appreciate any advice or opinions on true recycling VS. a filtering >> type of system. As we all know, experience with a system or product >> is the best way to gain true knowledge! >> >> Also, I would appreciate knowing how others are handling extra levels >> of prostate needle bx's. We currently place them on charged slides >> and keep until the case is signed out, but it seems there should be a >> better way of handling this due to storage of the slides not to >> mention the extra cost involved! I know this is becoming more of a >> needed process with all of the RNA/DNA testing being done on diagnosed cancers. >> >> As always, I do appreciate any and all comments, advice, or words of >> wisdom!! Hope to see some of you in Providence late next week! >> >> Dorothy Webb, HT (ASCP) >> Regions Histology Technical Specialist >> 651-254-2962 >> >> >> >> ________________________________ >> This e-mail and any files transmitted with it are confidential and >> are intended solely for the use of the individual or entity to whom >> they are addressed. If you are not the intended recipient or the >> individual responsible for delivering the e-mail to the intended >> recipient, please be advised that you have received this e-mail in >> error and that any use, dissemination, forwarding, printing, or >> copying of this e-mail is strictly prohibited. >> >> If you have received this communication in error, please return it to >> the sender immediately and delete the original message and any copy >> of it from your computer system. If you have any questions concerning >> this message, please contact the sender. Disclaimer R001.0 >> >> >> ------------------------------ >> >> Message: 6 >> Date: Thu, 12 Sep 2013 11:55:43 -0500 >> From: Tim Higgins >> Subject: [Histonet] RE: Histonet Digest, Vol 118, Issue 23 >> To: "histonet@lists.utsouthwestern.edu" >> >> Message-ID: >> Content-Type: text/plain; charset="iso-8859-1" >> >> Hey Martha, >> >> You have to forgive Rene J. she speaks sometimes and has nothing good >> to say but keeps speaking. There is never a bad question, if you >> don't have a good answer then keep it to yourself as I usually do. >> >> "Adequate forum". really? I guess no one on Histonet is allowed to >> share their personal protocols, procedures, opinions, and experiences >> on here anymore. >> >> Come on Rene J., next time shhhhhhhhh!! >> >> I should have just hit the "Delete" button and follow my own advise! >> >> Tim N. Higgins, HT (ASCP), QIHC >> >> >> > Message: 7 >> > Date: Thu, 12 Sep 2013 06:22:34 -0700 (PDT) >> > From: Rene J Buesa >> > Subject: Re: [Histonet] Minimum sample size for breast markers >> > To: Martha Ward-Pathology , >> > "histonet@lists.utsouthwestern.edu" >> > >> > Message-ID: >> > <1378992154.20733.YahooMailNeo@web163104.mail.bf1.yahoo.com> >> > Content-Type: text/plain; charset=iso-8859-1 >> > >> > I do not think this is the adequate forum to ask this question >> > because >> you may get a wrong answer that could determine your decision. >> > Your pathologist is the one who should decide about this issue. >> > Ren? J. >> > >> > >> > ________________________________ >> > From: Martha Ward-Pathology >> > To: "histonet@lists.utsouthwestern.edu" < >> histonet@lists.utsouthwestern.edu> >> > Sent: Thursday, September 12, 2013 9:06 AM >> > Subject: [Histonet] Minimum sample size for breast markers >> > >> > >> > >> > >> > Is there a minimum number of cells that should be present when >> > testing >> for ER, PR and Her2? I have looked on the CAP website but cannot see >> anything about this. We are constantly getting these small biopsies >> or FNAs for testing and I sometimes wonder what the lowest threshold >> is for >> testing...20 tumor cells, 50 tumor cells???? Any information would >> be appreciated. Thanks in advance for your help. >> > >> > Martha Ward, MT (ASCP) QIHC >> > Manager >> > >> > Molecular Diagnostics Lab >> > Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 >> > \ f 336.716.5890 mward@wakehealth.edu >> > >> >> >> ------------------------------ >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> End of Histonet Digest, Vol 118, Issue 24 >> ***************************************** >> > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From TNMayer <@t> mdanderson.org Thu Sep 12 13:22:39 2013 From: TNMayer <@t> mdanderson.org (Mayer,Toysha N) Date: Thu Sep 12 13:23:45 2013 Subject: [Histonet] RE: Equipment selection (Huggins, Haley - MRMC) Message-ID: <47E9B2C01DDDD94881EACD2DC44EBC8802B29FF2@D1PWPEXMBX05.mdanderson.edu> See if they can switch out something else for whatever you are unsure of. Add a cryostat, or cassette writer instead of the coverslipper if that is not what you want. They may go for that. Sincerely, Toysha N. Mayer, MBA, HT(ASCP) tnmayer@mdanderson.org Instructor/Education Coordinator Program in Histotechnology School of Health Professions MD Anderson Cancer Center 713-563.3481 To: Rene J Buesa , "histonet@lists.utsouthwestern.edu" Message-ID: <4F36EC93A5737D4F8A2974E8FB8E260615F5698C69@PHX-MSG-007-N2.chw.edu> Content-Type: text/plain; charset="iso-8859-1" I actually know all the equipment. Well, I know 3 of the pieces really well, the Leica Bond Max is new to me. I did go to another lab to see it as well as the Leica stainer/coverslipper. I was impressed with the Bond Max, not as much with the coverslipper part of the stainer/coverslipper. The big issue I am having is if I want to go with the Bond Max, I technically need to go with the Leica stainer/coverslipper because it is lumped into a big package deal. If I want the Sakura Prisma stainer/film coverslipper, I go with the Dako immunostainer. I am having trouble deciding. It is boiling down to money, which sucks since I might be forced to go with something that I might not be happy with in the future. Haley To: Huggins, Haley - MRMC; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Equipment selection I used the Sakura and the Dako, but it seems that the Leica Bond Max is getting popularity. Why don't you go to a lab where they are in use and take a look? Ren? J. From: "Huggins, Haley - MRMC" > To: "histonet@lists.utsouthwestern.edu" > Sent: Wednesday, September 11, 2013 4:46 PM Subject: [Histonet] Equipment selection Hi all, I am trying to decide on what equipment to purchase. I have picked the processor, microtomes, embedding centers and a few other pieces, but now I still need to decide on the H&E Stainer and the immunostainer. My options I have in front of me are for the H&E stainer are Sakura Prisma stainer/film coverslipper and the Leica ST5020-CV5030 stainer/glass coverslipper. For the Immunostainer, I am looking at the Dako Autostainer and the Leica Bond Max. Any advice on any of these instruments would be greatly appreciated. I am trying to finalize my decision pretty quickly. Thank you in advance for any input. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Thu Sep 12 13:40:09 2013 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Thu Sep 12 13:40:14 2013 Subject: [Histonet] Equipment selection In-Reply-To: <000801ceafe2$5fbdff80$1f39fe80$@xs4all.nl> References: <000801ceafe2$5fbdff80$1f39fe80$@xs4all.nl> Message-ID: Yes, agree. It is my personal favorite Joelle Weaver MAOM, HTL (ASCP) QIHC > From: pev@xs4all.nl > To: Haley.Huggins@DignityHealth.org > Date: Thu, 12 Sep 2013 20:03:22 +0200 > Subject: [Histonet] Equipment selection > CC: histonet@lists.utsouthwestern.edu > > Hi > > Maybe you know but sakura also offers a glas coverslipper in combination > with their prisma stainer. > > i have worked with both strainers and definitely would select the prisma. > the Leica system splashes (or drops) solutions on its way from bath to bath. > look at an older system and you will see stain drops where you don't expect > them. also there water bathes are assigned per position to a stain that is > way less flexible then the sakura system where the watherbathes form a groop > and the stainer selects which one is free. in our case we limmeted the > amount of different stains in both machines to allow higher HE trughput. > > I wil gladly supply you with more information. > > > > Piet visser > > > > ----------------------------------------- > > > > Hi all, > > > > I am trying to decide on what equipment to purchase. I have picked the > processor, microtomes, embedding centers and a few other pieces, but now I > still need to decide on the H&E Stainer and the immunostainer. My options I > have in front of me are for the H&E stainer are Sakura Prisma stainer/film > coverslipper and the Leica ST5020-CV5030 stainer/glass coverslipper. For the > Immunostainer, I am looking at the Dako Autostainer and the Leica Bond Max. > Any advice on any of these instruments would be greatly appreciated. I am > trying to finalize my decision pretty quickly. Thank you in advance for any > input. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BDeBrosse-Serra <@t> isisph.com Thu Sep 12 13:44:05 2013 From: BDeBrosse-Serra <@t> isisph.com (Bea DeBrosse-Serra) Date: Thu Sep 12 13:44:24 2013 Subject: [Histonet] Equipment selection In-Reply-To: <4F36EC93A5737D4F8A2974E8FB8E260615F5698C69@PHX-MSG-007-N2.chw.edu> References: <4F36EC93A5737D4F8A2974E8FB8E260615F5698BE8@PHX-MSG-007-N2.chw.edu> <1378989146.57715.YahooMailNeo@web163104.mail.bf1.yahoo.com> <4F36EC93A5737D4F8A2974E8FB8E260615F5698C69@PHX-MSG-007-N2.chw.edu> Message-ID: As far as stainer and coverslipper, I think it comes down to personal preference. I've used both, and I think both are very good. When it comes to Immunostainer, I'm familiar with Ventana, Dako, Thermo Scientific Autostainer (Labvision) and Bond Max. I do not like the Dako/Thermo platform at all. I really, really like the Bond Max. But then again, that is my personal preference and opinion. Bea Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Huggins, Haley - MRMC Sent: Thursday, September 12, 2013 8:56 AM To: Rene J Buesa; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Equipment selection I actually know all the equipment. Well, I know 3 of the pieces really well, the Leica Bond Max is new to me. I did go to another lab to see it as well as the Leica stainer/coverslipper. I was impressed with the Bond Max, not as much with the coverslipper part of the stainer/coverslipper. The big issue I am having is if I want to go with the Bond Max, I technically need to go with the Leica stainer/coverslipper because it is lumped into a big package deal. If I want the Sakura Prisma stainer/film coverslipper, I go with the Dako immunostainer. I am having trouble deciding. It is boiling down to money, which sucks since I might be forced to go with something that I might not be happy with in the future. Haley From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: Thursday, September 12, 2013 5:32 AM To: Huggins, Haley - MRMC; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Equipment selection I used the Sakura and the Dako, but it seems that the Leica Bond Max is getting popularity. Why don't you go to a lab where they are in use and take a look? Ren? J. From: "Huggins, Haley - MRMC" > To: "histonet@lists.utsouthwestern.edu" > Sent: Wednesday, September 11, 2013 4:46 PM Subject: [Histonet] Equipment selection Hi all, I am trying to decide on what equipment to purchase. I have picked the processor, microtomes, embedding centers and a few other pieces, but now I still need to decide on the H&E Stainer and the immunostainer. My options I have in front of me are for the H&E stainer are Sakura Prisma stainer/film coverslipper and the Leica ST5020-CV5030 stainer/glass coverslipper. For the Immunostainer, I am looking at the Dako Autostainer and the Leica Bond Max. Any advice on any of these instruments would be greatly appreciated. I am trying to finalize my decision pretty quickly. Thank you in advance for any input. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mward <@t> wakehealth.edu Thu Sep 12 14:19:31 2013 From: mward <@t> wakehealth.edu (Martha Ward-Pathology) Date: Thu Sep 12 14:19:40 2013 Subject: [Histonet] Re: Minimum sample size for breast markers In-Reply-To: References: Message-ID: Thanks to everyone that responded to my question. It has encouraged a nice discussion and I have learned from it. ? Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard ?\? Winston-Salem, NC 27157 p 336.716.2109 ?\? f 336.716.5890 ? mward@wakehealth.edu ? ? ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Thursday, September 12, 2013 1:56 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Minimum sample size for breast markers Pathologists need to be very ready to note that a breast cancer sample size is small, and that the markers need to be repeated on the lumpectomy or mastectomy specimen. This is easier said than done. Popping a Dolly-size breast into too-small container and dribbling a little formalin on it is not going to preserve the specimen adequately. Even with a moderate-sized lumpectomy specimen the formaldehyde penetration is going to be chancy. This is an issue that surgeons, OR nurses, and too many pathologists are unable to understand. The pathologist has to arrange to receive the specimen promptly and get the tumor cut into formalin. Easier said than done. The woman's cancer care depends on this getting done. It's non-trivial. And colon cancer is distinctly the next frontier for this problem. Bob Richmond Samurai Pathologist Maryville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Sep 12 15:33:10 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Sep 12 15:33:19 2013 Subject: [Histonet] Fat Stain on Paraffin Slide ? In-Reply-To: References: Message-ID: <1379017990.55039.YahooMailNeo@web163106.mail.bf1.yahoo.com> Sudan Black Ren? J. ________________________________ From: Stella Mireles To: "histonet@lists.utsouthwestern.edu" Sent: Thursday, September 12, 2013 1:15 PM Subject: [Histonet] Fat Stain on Paraffin Slide ? Ok Experts - Please advise me on a stain for Fat which I can do on paraffin sections? Could you expand on your own experience with such an animal. Thanks to All Stella Houston, Tx _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TNMayer <@t> mdanderson.org Thu Sep 12 15:36:38 2013 From: TNMayer <@t> mdanderson.org (Mayer,Toysha N) Date: Thu Sep 12 15:36:43 2013 Subject: [Histonet] RE: Histonet Digest, Vol 118, Issue 23 In-Reply-To: References: Message-ID: <47E9B2C01DDDD94881EACD2DC44EBC8802B2AA18@D1PWPEXMBX05.mdanderson.edu> To the Samurai Pathologist, I, in no way was attempting to 'lump you into a derogatory statement' about older pathologists. According to your posts, you value the knowledge and experience of the techs you encounter. I respect and applaud that. Wish we had more vocal ones like you. Please forgive me if it sounded that way. I am a big enough girl to apologize for an inadvertent insult. Not my intention. Some older pathologists have only been in one or two labs in their careers and are reluctant to realize that times have changed and that techs have to be acknowledged and recognized for their work. Sincerely, Toysha N. Mayer, MBA, HT(ASCP) tnmayer@mdanderson.org Instructor/Education Coordinator Program in Histotechnology School of Health Professions MD Anderson Cancer Center 713-563.3481 Message: 9 Date: Thu, 12 Sep 2013 13:41:44 +0000 From: "Marcum, Pamela A" Subject: RE: [Histonet] Re: Unregistered HT To: "'Bob Richmond'" , "Histonet@lists.utsouthwestern.edu" Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA32D31589F6@Mail2Node2.ad.uams.edu> Content-Type: text/plain; charset="us-ascii" Thank you Bob! I remember when pathology residents had to spend some time in histology learning at least enough to know we were a team not enemies. Residents now barely get to see the Histology Lab so expecting them to see us as anything other than labor is a part of the issue. Their world is changing and I am not sure how it will play out for AP as whole. Deming was very sure as you stated that worker feedback and more importantly input into the process of every area of manufacturing was the key to having a both a creative and happy workforce. Deming wanted to encourage creativity for the workers so they were part of the whole process. We are rarely asked for input in any area of the work arena due to the tiered system that has developed in all areas of employment today. It is usually the squeaky wheel that get the grease or in this improvements in any area of the workplace and life. Pam Marcum -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Thursday, September 12, 2013 8:22 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Unregistered HT Since somebody mentioned the Samurai Pathologist (who is now 74 years old and in his 50th year in pathology) - I agree with most of what's been said here and I won't repeat it. >From the pathologist's viewpoint - remember that most pathologists are >now on salary (or soon will be) and don't have a dog in the fight about doing the job as cheaply as possible. I think that a very large part of the problem is that most pathologists haven't a clue as to what goes on in the histology lab (that's why we cram cassettes full of fatty breast tissue), and that pathologists need to acquire this knowledge in residency, to the degree that they can teach and trouble-shoot or work with senior technologists who can. It's particularly important that pathologists learn to embed. Edwards Deming was an economist who grew up in "operations research" during World War 2. After the War he tried to get the automotive industry to adopt his methods. The executives laughed at him (and still do in the business schools, I think), so he took his ideas to Japan, where they built the Japanese automative industry. Deming's major idea (if I understand him correctly) was that workers need constant specific feedback about what they're doing. I think that the establishment of effective feedback from pathologist to histotechnologist is the first step in solving the problem we've been talking about. And I think that means a pathologist sitting down with a histotechnologist and reviewing some of the day's slides every blessed day. Bob Richmond Samurai Pathologist Maryville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From liz <@t> premierlab.com Thu Sep 12 15:40:41 2013 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Thu Sep 12 15:40:43 2013 Subject: [Histonet] Fat Stain on Paraffin Slide ? In-Reply-To: <1379017990.55039.YahooMailNeo@web163106.mail.bf1.yahoo.com> References: <1379017990.55039.YahooMailNeo@web163106.mail.bf1.yahoo.com> Message-ID: <14E2C6176416974295479C64A11CB9AE019BB56FE0E9@SBS2K8.premierlab.local> Forgive me if I'm missing something here but I'm under the impression that you can not stain for fat on paraffin section unless they have been rendered unsoluable via post fixation in something like osmium . The fat would be removed during the processing cycle in the alcohols and xylenes. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Laboratory Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 Work (303) 682-3949 Fax (303) 682-9060 Cell (303) 881-0763 liz@premierlab.com www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, September 12, 2013 2:33 PM To: Stella Mireles; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Fat Stain on Paraffin Slide ? Sudan Black Ren? J. ________________________________ From: Stella Mireles To: "histonet@lists.utsouthwestern.edu" Sent: Thursday, September 12, 2013 1:15 PM Subject: [Histonet] Fat Stain on Paraffin Slide ? Ok Experts - Please advise me on a stain for Fat which I can do on paraffin sections? Could you expand on your own experience with such an animal. Thanks to All Stella Houston, Tx _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Sep 12 15:49:24 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Sep 12 15:49:29 2013 Subject: [Histonet] Need your expertise In-Reply-To: <65365F35C0F2EF4D846EC3CA73E49C4302ACEFF2C823@HPEMX3.HealthPartners.int> References: <65365F35C0F2EF4D846EC3CA73E49C4302ACEFF2C823@HPEMX3.HealthPartners.int> Message-ID: <1379018964.91211.YahooMailNeo@web163102.mail.bf1.yahoo.com> Formalin is extremely dangerous, everybody know that, so my course of action has always been to interact with formalin the least possible. I did not recycle xylene, nor I recommend you to do that. Of the 2 possibilities you mention?the absolute worse is what you call "true recycling" of distillation. Not only you will be more exposed to the fumes but at the end you will have to work with the distillate in order to prepare NBF and later aliquot it. The second option (filtering) is less dangerous but you will have to collect the filtered liquid, check for its strength and aliquot it. The fact that you recycle formalin will not make you "greener" because, do you think that the specialized companies disposing of formalin dump it in the environment? They eliminate it in an innocuous form. Buy formalin prefilled vials and do not mess with it. Your health and that of your colleagues is worth more than a few dollars you are going to save. That is my sincere advise. Ren? J. ________________________________ From: "Webb, Dorothy L" To: "'histonet@lists.utsouthwestern.edu'" Sent: Thursday, September 12, 2013 12:45 PM Subject: [Histonet] Need your expertise We will finally be going green by recycling formalin.? I would appreciate any advice or opinions on true recycling VS. a filtering type of system. As we all know, experience with a system or product is the best way to gain true knowledge! Also, I would appreciate knowing how others are handling extra levels of prostate needle bx's.? We currently place them on charged slides and keep until the case is signed out, but it seems there should be a better way of handling this due to storage of the slides not to mention the extra cost involved! I know this is becoming more of a needed process with all of the RNA/DNA testing being done on diagnosed cancers. As always, I do appreciate any and all comments, advice, or words of wisdom!!? Hope to see some of you in Providence late next week! Dorothy Webb, HT (ASCP) Regions Histology Technical Specialist 651-254-2962 ? ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From llewllew <@t> shaw.ca Thu Sep 12 15:54:01 2013 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Thu Sep 12 15:54:12 2013 Subject: [Histonet] Fat Stain on Paraffin Slide ? In-Reply-To: <14E2C6176416974295479C64A11CB9AE019BB56FE0E9@SBS2K8.premierlab.local> References: <1379017990.55039.YahooMailNeo@web163106.mail.bf1.yahoo.com> <14E2C6176416974295479C64A11CB9AE019BB56FE0E9@SBS2K8.premierlab.local> Message-ID: <523229E9.5070709@shaw.ca> I always used Chiffele and Putt's oil red O in propylene glycol for paraffin sections(http://stainsfile.info/StainsFile/stain/lipid/chiffelleputtpropgly.htm). Althugh triglycerides (i.e. "fat") is removed from tissues during paraffin processing, some lipids are not, or are only partially removed. Lipofuscins are an exaample, so is myelin to a lesser degree. usually the resistant lipids are those bound to protein. Oil red O is actually a good stain for lipofuscin. Of course, do not dehydrate or clear the section after staining, and use an aqueous mounting medium. Bryan Llewellyn Elizabeth Chlipala wrote: > Forgive me if I'm missing something here but I'm under the impression that you can not stain for fat on paraffin section unless they have been rendered unsoluable via post fixation in something like osmium . The fat would be removed during the processing cycle in the alcohols and xylenes. > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > Laboratory Manager > Premier Laboratory, LLC > PO Box 18592 > Boulder, CO 80308 > Work (303) 682-3949 > Fax (303) 682-9060 > Cell (303) 881-0763 > liz@premierlab.com > www.premierlab.com > > Ship to address: > > 1567 Skyway Drive, Unit E > Longmont, CO 80504 > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa > Sent: Thursday, September 12, 2013 2:33 PM > To: Stella Mireles; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Fat Stain on Paraffin Slide ? > > Sudan Black > Ren? J. > > > ________________________________ > From: Stella Mireles > To: "histonet@lists.utsouthwestern.edu" > Sent: Thursday, September 12, 2013 1:15 PM > Subject: [Histonet] Fat Stain on Paraffin Slide ? > > > Ok Experts - Please advise me on a stain for Fat which I can do on paraffin sections? > Could you expand on your own experience with such an animal. > > Thanks to All > Stella > Houston, Tx > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From rjbuesa <@t> yahoo.com Thu Sep 12 15:59:31 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Sep 12 15:59:36 2013 Subject: [Histonet] Fat Stain on Paraffin Slide ? In-Reply-To: <14E2C6176416974295479C64A11CB9AE019BB56FE0E9@SBS2K8.premierlab.local> References: <1379017990.55039.YahooMailNeo@web163106.mail.bf1.yahoo.com> <14E2C6176416974295479C64A11CB9AE019BB56FE0E9@SBS2K8.premierlab.local> Message-ID: <1379019571.15841.YahooMailNeo@web163103.mail.bf1.yahoo.com> Sudan Black?stains lipofucsins in FFPE tissues. If you add osmium tetroxide to a tissue after fixation it will fix/stain the fats black?and that stain will "survive" the processing protocol. Ren? J.? ________________________________ From: Elizabeth Chlipala To: Rene J Buesa ; Stella Mireles ; "histonet@lists.utsouthwestern.edu" Sent: Thursday, September 12, 2013 4:40 PM Subject: RE: [Histonet] Fat Stain on Paraffin Slide ? Forgive me if I'm missing something here but I'm under the impression that you can not stain for fat on paraffin section unless they have been rendered unsoluable via post fixation in something like osmium .? The fat would be removed during the processing cycle in the alcohols and xylenes. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Laboratory Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 Work (303) 682-3949 Fax (303) 682-9060 Cell (303) 881-0763 liz@premierlab.com www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, September 12, 2013 2:33 PM To: Stella Mireles; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Fat Stain on Paraffin Slide ? Sudan Black Ren? J. ________________________________ From: Stella Mireles To: "histonet@lists.utsouthwestern.edu" Sent: Thursday, September 12, 2013 1:15 PM Subject: [Histonet] Fat Stain on Paraffin Slide ? Ok Experts - Please advise me on a stain for Fat which I can do on paraffin sections? Could you expand on your own experience with such an animal. Thanks to All Stella Houston, Tx _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amber.mckenzie <@t> gastrodocs.net Thu Sep 12 17:03:23 2013 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Thu Sep 12 17:03:29 2013 Subject: [Histonet] Redesigning a lab In-Reply-To: <1379019571.15841.YahooMailNeo@web163103.mail.bf1.yahoo.com> References: <1379017990.55039.YahooMailNeo@web163106.mail.bf1.yahoo.com> <14E2C6176416974295479C64A11CB9AE019BB56FE0E9@SBS2K8.premierlab.local> <1379019571.15841.YahooMailNeo@web163103.mail.bf1.yahoo.com> Message-ID: <5A33C952BB67F4468AF1F36D739212BCBD6E50FC@JERRY.Gia.com> I am currently redesigning our GI lab. I wanted everyone's opinion on if you could redesign your lab, what would you change/add/delete? Would you do counters high or low? What flooring would you use? What brand/type of countertop would you use? I'm trying to design it for lots of extra space for growth, plus a room attached for blocks/slides for 10 years! Any suggestions? Do most of you have a DI tap in your lab sink or did you buy a water system you have to use or do you buy your water weekly from a supplier? Thanks! From annigyg <@t> gmail.com Fri Sep 13 00:46:55 2013 From: annigyg <@t> gmail.com (Anne) Date: Fri Sep 13 00:47:14 2013 Subject: [Histonet] Re: Histonet Digest, Vol 118, Issue 24 In-Reply-To: <5231f38d.e9363c0a.4773.ffffb744SMTPIN_ADDED_MISSING@mx.google.com> References: <5231f38d.e9363c0a.4773.ffffb744SMTPIN_ADDED_MISSING@mx.google.com> Message-ID: <605D7189-8F2B-404F-A0F6-2516A651D0B1@gmail.com> Choose the FILM coverslipper. Sakura and DAKO over Leica anytime. Annieinarabia Sent from my iPhone On 12 Sep 2013, at 9:02 PM, histonet-request@lists.utsouthwestern.edu wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. RE: Equipment selection (Huggins, Haley - MRMC) > 2. Re: Equipment selection (Rene J Buesa) > 3. RE: Equipment selection (Huggins, Haley - MRMC) > 4. RE: Equipment selection (Marcum, Pamela A) > 5. Need your expertise (Webb, Dorothy L) > 6. RE: Histonet Digest, Vol 118, Issue 23 (Tim Higgins) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Thu, 12 Sep 2013 08:55:39 -0700 > From: "Huggins, Haley - MRMC" > Subject: RE: [Histonet] Equipment selection > To: Rene J Buesa , > "histonet@lists.utsouthwestern.edu" > > Message-ID: > <4F36EC93A5737D4F8A2974E8FB8E260615F5698C69@PHX-MSG-007-N2.chw.edu> > Content-Type: text/plain; charset="iso-8859-1" > > I actually know all the equipment. Well, I know 3 of the pieces really well, the Leica Bond Max is new to me. I did go to another lab to see it as well as the Leica stainer/coverslipper. I was impressed with the Bond Max, not as much with the coverslipper part of the stainer/coverslipper. The big issue I am having is if I want to go with the Bond Max, I technically need to go with the Leica stainer/coverslipper because it is lumped into a big package deal. If I want the Sakura Prisma stainer/film coverslipper, I go with the Dako immunostainer. I am having trouble deciding. It is boiling down to money, which sucks since I might be forced to go with something that I might not be happy with in the future. > > Haley > > From: Rene J Buesa [mailto:rjbuesa@yahoo.com] > Sent: Thursday, September 12, 2013 5:32 AM > To: Huggins, Haley - MRMC; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Equipment selection > > I used the Sakura and the Dako, but it seems that the Leica Bond Max is getting popularity. Why don't you go to a lab where they are in use and take a look? > Ren? J. > > From: "Huggins, Haley - MRMC" > > To: "histonet@lists.utsouthwestern.edu" > > Sent: Wednesday, September 11, 2013 4:46 PM > Subject: [Histonet] Equipment selection > > Hi all, > > I am trying to decide on what equipment to purchase. I have picked the processor, microtomes, embedding centers and a few other pieces, but now I still need to decide on the H&E Stainer and the immunostainer. My options I have in front of me are for the H&E stainer are Sakura Prisma stainer/film coverslipper and the Leica ST5020-CV5030 stainer/glass coverslipper. For the Immunostainer, I am looking at the Dako Autostainer and the Leica Bond Max. Any advice on any of these instruments would be greatly appreciated. I am trying to finalize my decision pretty quickly. Thank you in advance for any input. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Message: 2 > Date: Thu, 12 Sep 2013 09:07:56 -0700 (PDT) > From: Rene J Buesa > Subject: Re: [Histonet] Equipment selection > To: "Huggins, Haley - MRMC" , > "histonet@lists.utsouthwestern.edu" > > Message-ID: > <1379002076.53612.YahooMailNeo@web163105.mail.bf1.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > If buying the Bond means that you are forced to "swallow" the Leica coverslipper go with Sakura and Dako. > If they are making that "package" is because the Bond sells well but they are having to "swallow" the coverslipper. Price is important but in the long run it is better to increase productivity and avoid problems. The Sakura is much faster than the Leica coverslipper. > Ren? J. > > > ________________________________ > From: "Huggins, Haley - MRMC" > To: Rene J Buesa ; "histonet@lists.utsouthwestern.edu" > Sent: Thursday, September 12, 2013 11:55 AM > Subject: RE: [Histonet] Equipment selection > > > > I actually know all the equipment. Well, I know 3 of the pieces really well, the Leica Bond Max is new to me. I did go to another lab to see it as well as the Leica stainer/coverslipper. I was impressed with the Bond Max, not as much with the coverslipper part of the stainer/coverslipper. The big issue I am having is if I want to go with the Bond Max, I technically need to go with the Leica stainer/coverslipper because it is lumped into a big package deal. If I want the Sakura Prisma stainer/film coverslipper, I go with the Dako immunostainer. I am having trouble deciding. It is boiling down to money, which sucks since I might be forced to go with something that I might not be happy with in the future. > > Haley > > From:Rene J Buesa [mailto:rjbuesa@yahoo.com] > Sent: Thursday, September 12, 2013 5:32 AM > To: Huggins, Haley - MRMC; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Equipment selection > > I used the Sakura and the Dako, but it seems that the Leica Bond Max is getting popularity. Why don't you go to a lab where they are in use and take a look? > Ren?J. > > From:"Huggins, Haley - MRMC" > To: "histonet@lists.utsouthwestern.edu" > Sent: Wednesday, September 11, 2013 4:46 PM > Subject: [Histonet] Equipment selection > > Hi all, > > I am trying to decide on what equipment to purchase. I have picked the processor, microtomes, embedding centers and a few other pieces, but now I still need to decide on the H&E Stainer and the immunostainer. My options I have in front of me are for the H&E stainer are Sakura Prisma stainer/film coverslipper and the Leica ST5020-CV5030 stainer/glass coverslipper. For the Immunostainer, I am looking at the Dako Autostainer and the Leica Bond Max. Any advice on any of these instruments would be greatly appreciated. I am trying to finalize my decision pretty quickly. Thank you in advance for any input. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > Message: 3 > Date: Thu, 12 Sep 2013 09:10:47 -0700 > From: "Huggins, Haley - MRMC" > Subject: RE: [Histonet] Equipment selection > To: Rene J Buesa , > "histonet@lists.utsouthwestern.edu" > > Message-ID: > <4F36EC93A5737D4F8A2974E8FB8E260615F5698C6D@PHX-MSG-007-N2.chw.edu> > Content-Type: text/plain; charset="iso-8859-1" > > Thanks, that is a good point. > > From: Rene J Buesa [mailto:rjbuesa@yahoo.com] > Sent: Thursday, September 12, 2013 9:08 AM > To: Huggins, Haley - MRMC; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Equipment selection > > If buying the Bond means that you are forced to "swallow" the Leica coverslipper go with Sakura and Dako. > If they are making that "package" is because the Bond sells well but they are having to "swallow" the coverslipper. Price is important but in the long run it is better to increase productivity and avoid problems. The Sakura is much faster than the Leica coverslipper. > Ren? J. > > From: "Huggins, Haley - MRMC" > > To: Rene J Buesa >; "histonet@lists.utsouthwestern.edu" > > Sent: Thursday, September 12, 2013 11:55 AM > Subject: RE: [Histonet] Equipment selection > > I actually know all the equipment. Well, I know 3 of the pieces really well, the Leica Bond Max is new to me. I did go to another lab to see it as well as the Leica stainer/coverslipper. I was impressed with the Bond Max, not as much with the coverslipper part of the stainer/coverslipper. The big issue I am having is if I want to go with the Bond Max, I technically need to go with the Leica stainer/coverslipper because it is lumped into a big package deal. If I want the Sakura Prisma stainer/film coverslipper, I go with the Dako immunostainer. I am having trouble deciding. It is boiling down to money, which sucks since I might be forced to go with something that I might not be happy with in the future. > > Haley > > From: Rene J Buesa [mailto:rjbuesa@yahoo.com] > Sent: Thursday, September 12, 2013 5:32 AM > To: Huggins, Haley - MRMC; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Equipment selection > > I used the Sakura and the Dako, but it seems that the Leica Bond Max is getting popularity. Why don't you go to a lab where they are in use and take a look? > Ren? J. > > From: "Huggins, Haley - MRMC" > > To: "histonet@lists.utsouthwestern.edu" > > Sent: Wednesday, September 11, 2013 4:46 PM > Subject: [Histonet] Equipment selection > > Hi all, > > I am trying to decide on what equipment to purchase. I have picked the processor, microtomes, embedding centers and a few other pieces, but now I still need to decide on the H&E Stainer and the immunostainer. My options I have in front of me are for the H&E stainer are Sakura Prisma stainer/film coverslipper and the Leica ST5020-CV5030 stainer/glass coverslipper. For the Immunostainer, I am looking at the Dako Autostainer and the Leica Bond Max. Any advice on any of these instruments would be greatly appreciated. I am trying to finalize my decision pretty quickly. Thank you in advance for any input. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 4 > Date: Thu, 12 Sep 2013 16:30:13 +0000 > From: "Marcum, Pamela A" > Subject: RE: [Histonet] Equipment selection > To: "'Rene J Buesa'" , "Huggins, Haley - MRMC" > , "histonet@lists.utsouthwestern.edu" > > Message-ID: > <41D3A1AF6FEF0643BDC89E0516A6EA32D3158B57@Mail2Node2.ad.uams.edu> > Content-Type: text/plain; charset="iso-8859-1" > > Actually the coverslipper from Leica is very good and can be linked to the stainer. It is also a glass coverslipper not tape. It is always about money and that may be the thing that decides no matter what any of prefers. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa > Sent: Thursday, September 12, 2013 11:08 AM > To: Huggins, Haley - MRMC; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Equipment selection > > If buying the Bond means that you are forced to "swallow" the Leica coverslipper go with Sakura and Dako. > If they are making that "package" is because the Bond sells well but they are having to "swallow" the coverslipper. Price is important but in the long run it is better to increase productivity and avoid problems. The Sakura is much faster than the Leica coverslipper. > Ren? J. > > > ________________________________ > From: "Huggins, Haley - MRMC" > To: Rene J Buesa ; "histonet@lists.utsouthwestern.edu" > Sent: Thursday, September 12, 2013 11:55 AM > Subject: RE: [Histonet] Equipment selection > > > > I actually know all the equipment. Well, I know 3 of the pieces really well, the Leica Bond Max is new to me. I did go to another lab to see it as well as the Leica stainer/coverslipper. I was impressed with the Bond Max, not as much with the coverslipper part of the stainer/coverslipper. The big issue I am having is if I want to go with the Bond Max, I technically need to go with the Leica stainer/coverslipper because it is lumped into a big package deal. If I want the Sakura Prisma stainer/film coverslipper, I go with the Dako immunostainer. I am having trouble deciding. It is boiling down to money, which sucks since I might be forced to go with something that I might not be happy with in the future. > > Haley > > From:Rene J Buesa [mailto:rjbuesa@yahoo.com] > Sent: Thursday, September 12, 2013 5:32 AM > To: Huggins, Haley - MRMC; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Equipment selection > > I used the Sakura and the Dako, but it seems that the Leica Bond Max is getting popularity. Why don't you go to a lab where they are in use and take a look? > Ren?J. > > From:"Huggins, Haley - MRMC" > To: "histonet@lists.utsouthwestern.edu" > Sent: Wednesday, September 11, 2013 4:46 PM > Subject: [Histonet] Equipment selection > > Hi all, > > I am trying to decide on what equipment to purchase. I have picked the processor, microtomes, embedding centers and a few other pieces, but now I still need to decide on the H&E Stainer and the immunostainer. My options I have in front of me are for the H&E stainer are Sakura Prisma stainer/film coverslipper and the Leica ST5020-CV5030 stainer/glass coverslipper. For the Immunostainer, I am looking at the Dako Autostainer and the Leica Bond Max. Any advice on any of these instruments would be greatly appreciated. I am trying to finalize my decision pretty quickly. Thank you in advance for any input. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ---------------------------------------------------------------------- > Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. > > > > ------------------------------ > > Message: 5 > Date: Thu, 12 Sep 2013 11:45:41 -0500 > From: "Webb, Dorothy L" > Subject: [Histonet] Need your expertise > To: "'histonet@lists.utsouthwestern.edu'" > > Message-ID: > <65365F35C0F2EF4D846EC3CA73E49C4302ACEFF2C823@HPEMX3.HealthPartners.int> > > Content-Type: text/plain; charset="us-ascii" > > We will finally be going green by recycling formalin. I would appreciate any advice or opinions on true recycling VS. a filtering type of system. As we all know, experience with a system or product is the best way to gain true knowledge! > > Also, I would appreciate knowing how others are handling extra levels of prostate needle bx's. We currently place them on charged slides and keep until the case is signed out, but it seems there should be a better way of handling this due to storage of the slides not to mention the extra cost involved! I know this is becoming more of a needed process with all of the RNA/DNA testing being done on diagnosed cancers. > > As always, I do appreciate any and all comments, advice, or words of wisdom!! Hope to see some of you in Providence late next week! > > Dorothy Webb, HT (ASCP) > Regions Histology Technical Specialist > 651-254-2962 > > > > ________________________________ > This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. > > If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 > > > ------------------------------ > > Message: 6 > Date: Thu, 12 Sep 2013 11:55:43 -0500 > From: Tim Higgins > Subject: [Histonet] RE: Histonet Digest, Vol 118, Issue 23 > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > Hey Martha, > > You have to forgive Rene J. she speaks sometimes and has nothing good to say but keeps speaking. There is never a bad question, if you don't have a good answer then keep it to yourself as I usually do. > > "Adequate forum". really? I guess no one on Histonet is allowed to share their personal protocols, procedures, opinions, and experiences on here anymore. > > Come on Rene J., next time shhhhhhhhh!! > > I should have just hit the "Delete" button and follow my own advise! > > Tim N. Higgins, HT (ASCP), QIHC > > >> Message: 7 >> Date: Thu, 12 Sep 2013 06:22:34 -0700 (PDT) >> From: Rene J Buesa >> Subject: Re: [Histonet] Minimum sample size for breast markers >> To: Martha Ward-Pathology , >> "histonet@lists.utsouthwestern.edu" >> >> Message-ID: >> <1378992154.20733.YahooMailNeo@web163104.mail.bf1.yahoo.com> >> Content-Type: text/plain; charset=iso-8859-1 >> >> I do not think this is the adequate forum to ask this question because you may get a wrong answer that could determine your decision. >> Your pathologist is the one who should decide about this issue. >> Ren? J. >> >> >> ________________________________ >> From: Martha Ward-Pathology >> To: "histonet@lists.utsouthwestern.edu" >> Sent: Thursday, September 12, 2013 9:06 AM >> Subject: [Histonet] Minimum sample size for breast markers >> >> >> >> >> Is there a minimum number of cells that should be present when testing for ER, PR and Her2? I have looked on the CAP website but cannot see anything about this. We are constantly getting these small biopsies or FNAs for testing and I sometimes wonder what the lowest threshold is for testing...20 tumor cells, 50 tumor cells???? Any information would be appreciated. Thanks in advance for your help. >> >> Martha Ward, MT (ASCP) QIHC >> Manager >> >> Molecular Diagnostics Lab >> Medical Center Boulevard \ Winston-Salem, NC 27157 >> p 336.716.2109 \ f 336.716.5890 >> mward@wakehealth.edu > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 118, Issue 24 > ***************************************** From annigyg <@t> gmail.com Fri Sep 13 00:52:45 2013 From: annigyg <@t> gmail.com (Anne) Date: Fri Sep 13 00:53:01 2013 Subject: [Histonet] Re: Histonet Digest, Vol 118, Issue 24 In-Reply-To: <5231f38d.e9363c0a.4773.ffffb744SMTPIN_ADDED_MISSING@mx.google.com> References: <5231f38d.e9363c0a.4773.ffffb744SMTPIN_ADDED_MISSING@mx.google.com> Message-ID: My goodness ignorance abounds...Rene J is one of the most respected voices on this forum. And 'she' is actually a 'he' you dimwits. I would take his advice above almost anyone on this forum. You youngsters should try to emulate rather than denigrate. Rene you rock!!! Annieinarabia Sent from my iPhone On 12 Sep 2013, at 9:02 PM, histonet-request@lists.utsouthwestern.edu wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. RE: Equipment selection (Huggins, Haley - MRMC) > 2. Re: Equipment selection (Rene J Buesa) > 3. RE: Equipment selection (Huggins, Haley - MRMC) > 4. RE: Equipment selection (Marcum, Pamela A) > 5. Need your expertise (Webb, Dorothy L) > 6. RE: Histonet Digest, Vol 118, Issue 23 (Tim Higgins) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Thu, 12 Sep 2013 08:55:39 -0700 > From: "Huggins, Haley - MRMC" > Subject: RE: [Histonet] Equipment selection > To: Rene J Buesa , > "histonet@lists.utsouthwestern.edu" > > Message-ID: > <4F36EC93A5737D4F8A2974E8FB8E260615F5698C69@PHX-MSG-007-N2.chw.edu> > Content-Type: text/plain; charset="iso-8859-1" > > I actually know all the equipment. Well, I know 3 of the pieces really well, the Leica Bond Max is new to me. I did go to another lab to see it as well as the Leica stainer/coverslipper. I was impressed with the Bond Max, not as much with the coverslipper part of the stainer/coverslipper. The big issue I am having is if I want to go with the Bond Max, I technically need to go with the Leica stainer/coverslipper because it is lumped into a big package deal. If I want the Sakura Prisma stainer/film coverslipper, I go with the Dako immunostainer. I am having trouble deciding. It is boiling down to money, which sucks since I might be forced to go with something that I might not be happy with in the future. > > Haley > > From: Rene J Buesa [mailto:rjbuesa@yahoo.com] > Sent: Thursday, September 12, 2013 5:32 AM > To: Huggins, Haley - MRMC; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Equipment selection > > I used the Sakura and the Dako, but it seems that the Leica Bond Max is getting popularity. Why don't you go to a lab where they are in use and take a look? > Ren? J. > > From: "Huggins, Haley - MRMC" > > To: "histonet@lists.utsouthwestern.edu" > > Sent: Wednesday, September 11, 2013 4:46 PM > Subject: [Histonet] Equipment selection > > Hi all, > > I am trying to decide on what equipment to purchase. I have picked the processor, microtomes, embedding centers and a few other pieces, but now I still need to decide on the H&E Stainer and the immunostainer. My options I have in front of me are for the H&E stainer are Sakura Prisma stainer/film coverslipper and the Leica ST5020-CV5030 stainer/glass coverslipper. For the Immunostainer, I am looking at the Dako Autostainer and the Leica Bond Max. Any advice on any of these instruments would be greatly appreciated. I am trying to finalize my decision pretty quickly. Thank you in advance for any input. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Message: 2 > Date: Thu, 12 Sep 2013 09:07:56 -0700 (PDT) > From: Rene J Buesa > Subject: Re: [Histonet] Equipment selection > To: "Huggins, Haley - MRMC" , > "histonet@lists.utsouthwestern.edu" > > Message-ID: > <1379002076.53612.YahooMailNeo@web163105.mail.bf1.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > If buying the Bond means that you are forced to "swallow" the Leica coverslipper go with Sakura and Dako. > If they are making that "package" is because the Bond sells well but they are having to "swallow" the coverslipper. Price is important but in the long run it is better to increase productivity and avoid problems. The Sakura is much faster than the Leica coverslipper. > Ren? J. > > > ________________________________ > From: "Huggins, Haley - MRMC" > To: Rene J Buesa ; "histonet@lists.utsouthwestern.edu" > Sent: Thursday, September 12, 2013 11:55 AM > Subject: RE: [Histonet] Equipment selection > > > > I actually know all the equipment. Well, I know 3 of the pieces really well, the Leica Bond Max is new to me. I did go to another lab to see it as well as the Leica stainer/coverslipper. I was impressed with the Bond Max, not as much with the coverslipper part of the stainer/coverslipper. The big issue I am having is if I want to go with the Bond Max, I technically need to go with the Leica stainer/coverslipper because it is lumped into a big package deal. If I want the Sakura Prisma stainer/film coverslipper, I go with the Dako immunostainer. I am having trouble deciding. It is boiling down to money, which sucks since I might be forced to go with something that I might not be happy with in the future. > > Haley > > From:Rene J Buesa [mailto:rjbuesa@yahoo.com] > Sent: Thursday, September 12, 2013 5:32 AM > To: Huggins, Haley - MRMC; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Equipment selection > > I used the Sakura and the Dako, but it seems that the Leica Bond Max is getting popularity. Why don't you go to a lab where they are in use and take a look? > Ren?J. > > From:"Huggins, Haley - MRMC" > To: "histonet@lists.utsouthwestern.edu" > Sent: Wednesday, September 11, 2013 4:46 PM > Subject: [Histonet] Equipment selection > > Hi all, > > I am trying to decide on what equipment to purchase. I have picked the processor, microtomes, embedding centers and a few other pieces, but now I still need to decide on the H&E Stainer and the immunostainer. My options I have in front of me are for the H&E stainer are Sakura Prisma stainer/film coverslipper and the Leica ST5020-CV5030 stainer/glass coverslipper. For the Immunostainer, I am looking at the Dako Autostainer and the Leica Bond Max. Any advice on any of these instruments would be greatly appreciated. I am trying to finalize my decision pretty quickly. Thank you in advance for any input. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > Message: 3 > Date: Thu, 12 Sep 2013 09:10:47 -0700 > From: "Huggins, Haley - MRMC" > Subject: RE: [Histonet] Equipment selection > To: Rene J Buesa , > "histonet@lists.utsouthwestern.edu" > > Message-ID: > <4F36EC93A5737D4F8A2974E8FB8E260615F5698C6D@PHX-MSG-007-N2.chw.edu> > Content-Type: text/plain; charset="iso-8859-1" > > Thanks, that is a good point. > > From: Rene J Buesa [mailto:rjbuesa@yahoo.com] > Sent: Thursday, September 12, 2013 9:08 AM > To: Huggins, Haley - MRMC; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Equipment selection > > If buying the Bond means that you are forced to "swallow" the Leica coverslipper go with Sakura and Dako. > If they are making that "package" is because the Bond sells well but they are having to "swallow" the coverslipper. Price is important but in the long run it is better to increase productivity and avoid problems. The Sakura is much faster than the Leica coverslipper. > Ren? J. > > From: "Huggins, Haley - MRMC" > > To: Rene J Buesa >; "histonet@lists.utsouthwestern.edu" > > Sent: Thursday, September 12, 2013 11:55 AM > Subject: RE: [Histonet] Equipment selection > > I actually know all the equipment. Well, I know 3 of the pieces really well, the Leica Bond Max is new to me. I did go to another lab to see it as well as the Leica stainer/coverslipper. I was impressed with the Bond Max, not as much with the coverslipper part of the stainer/coverslipper. The big issue I am having is if I want to go with the Bond Max, I technically need to go with the Leica stainer/coverslipper because it is lumped into a big package deal. If I want the Sakura Prisma stainer/film coverslipper, I go with the Dako immunostainer. I am having trouble deciding. It is boiling down to money, which sucks since I might be forced to go with something that I might not be happy with in the future. > > Haley > > From: Rene J Buesa [mailto:rjbuesa@yahoo.com] > Sent: Thursday, September 12, 2013 5:32 AM > To: Huggins, Haley - MRMC; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Equipment selection > > I used the Sakura and the Dako, but it seems that the Leica Bond Max is getting popularity. Why don't you go to a lab where they are in use and take a look? > Ren? J. > > From: "Huggins, Haley - MRMC" > > To: "histonet@lists.utsouthwestern.edu" > > Sent: Wednesday, September 11, 2013 4:46 PM > Subject: [Histonet] Equipment selection > > Hi all, > > I am trying to decide on what equipment to purchase. I have picked the processor, microtomes, embedding centers and a few other pieces, but now I still need to decide on the H&E Stainer and the immunostainer. My options I have in front of me are for the H&E stainer are Sakura Prisma stainer/film coverslipper and the Leica ST5020-CV5030 stainer/glass coverslipper. For the Immunostainer, I am looking at the Dako Autostainer and the Leica Bond Max. Any advice on any of these instruments would be greatly appreciated. I am trying to finalize my decision pretty quickly. Thank you in advance for any input. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 4 > Date: Thu, 12 Sep 2013 16:30:13 +0000 > From: "Marcum, Pamela A" > Subject: RE: [Histonet] Equipment selection > To: "'Rene J Buesa'" , "Huggins, Haley - MRMC" > , "histonet@lists.utsouthwestern.edu" > > Message-ID: > <41D3A1AF6FEF0643BDC89E0516A6EA32D3158B57@Mail2Node2.ad.uams.edu> > Content-Type: text/plain; charset="iso-8859-1" > > Actually the coverslipper from Leica is very good and can be linked to the stainer. It is also a glass coverslipper not tape. It is always about money and that may be the thing that decides no matter what any of prefers. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa > Sent: Thursday, September 12, 2013 11:08 AM > To: Huggins, Haley - MRMC; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Equipment selection > > If buying the Bond means that you are forced to "swallow" the Leica coverslipper go with Sakura and Dako. > If they are making that "package" is because the Bond sells well but they are having to "swallow" the coverslipper. Price is important but in the long run it is better to increase productivity and avoid problems. The Sakura is much faster than the Leica coverslipper. > Ren? J. > > > ________________________________ > From: "Huggins, Haley - MRMC" > To: Rene J Buesa ; "histonet@lists.utsouthwestern.edu" > Sent: Thursday, September 12, 2013 11:55 AM > Subject: RE: [Histonet] Equipment selection > > > > I actually know all the equipment. Well, I know 3 of the pieces really well, the Leica Bond Max is new to me. I did go to another lab to see it as well as the Leica stainer/coverslipper. I was impressed with the Bond Max, not as much with the coverslipper part of the stainer/coverslipper. The big issue I am having is if I want to go with the Bond Max, I technically need to go with the Leica stainer/coverslipper because it is lumped into a big package deal. If I want the Sakura Prisma stainer/film coverslipper, I go with the Dako immunostainer. I am having trouble deciding. It is boiling down to money, which sucks since I might be forced to go with something that I might not be happy with in the future. > > Haley > > From:Rene J Buesa [mailto:rjbuesa@yahoo.com] > Sent: Thursday, September 12, 2013 5:32 AM > To: Huggins, Haley - MRMC; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Equipment selection > > I used the Sakura and the Dako, but it seems that the Leica Bond Max is getting popularity. Why don't you go to a lab where they are in use and take a look? > Ren?J. > > From:"Huggins, Haley - MRMC" > To: "histonet@lists.utsouthwestern.edu" > Sent: Wednesday, September 11, 2013 4:46 PM > Subject: [Histonet] Equipment selection > > Hi all, > > I am trying to decide on what equipment to purchase. I have picked the processor, microtomes, embedding centers and a few other pieces, but now I still need to decide on the H&E Stainer and the immunostainer. My options I have in front of me are for the H&E stainer are Sakura Prisma stainer/film coverslipper and the Leica ST5020-CV5030 stainer/glass coverslipper. For the Immunostainer, I am looking at the Dako Autostainer and the Leica Bond Max. Any advice on any of these instruments would be greatly appreciated. I am trying to finalize my decision pretty quickly. Thank you in advance for any input. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ---------------------------------------------------------------------- > Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. > > > > ------------------------------ > > Message: 5 > Date: Thu, 12 Sep 2013 11:45:41 -0500 > From: "Webb, Dorothy L" > Subject: [Histonet] Need your expertise > To: "'histonet@lists.utsouthwestern.edu'" > > Message-ID: > <65365F35C0F2EF4D846EC3CA73E49C4302ACEFF2C823@HPEMX3.HealthPartners.int> > > Content-Type: text/plain; charset="us-ascii" > > We will finally be going green by recycling formalin. I would appreciate any advice or opinions on true recycling VS. a filtering type of system. As we all know, experience with a system or product is the best way to gain true knowledge! > > Also, I would appreciate knowing how others are handling extra levels of prostate needle bx's. We currently place them on charged slides and keep until the case is signed out, but it seems there should be a better way of handling this due to storage of the slides not to mention the extra cost involved! I know this is becoming more of a needed process with all of the RNA/DNA testing being done on diagnosed cancers. > > As always, I do appreciate any and all comments, advice, or words of wisdom!! Hope to see some of you in Providence late next week! > > Dorothy Webb, HT (ASCP) > Regions Histology Technical Specialist > 651-254-2962 > > > > ________________________________ > This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. > > If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 > > > ------------------------------ > > Message: 6 > Date: Thu, 12 Sep 2013 11:55:43 -0500 > From: Tim Higgins > Subject: [Histonet] RE: Histonet Digest, Vol 118, Issue 23 > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > Hey Martha, > > You have to forgive Rene J. she speaks sometimes and has nothing good to say but keeps speaking. There is never a bad question, if you don't have a good answer then keep it to yourself as I usually do. > > "Adequate forum". really? I guess no one on Histonet is allowed to share their personal protocols, procedures, opinions, and experiences on here anymore. > > Come on Rene J., next time shhhhhhhhh!! > > I should have just hit the "Delete" button and follow my own advise! > > Tim N. Higgins, HT (ASCP), QIHC > > >> Message: 7 >> Date: Thu, 12 Sep 2013 06:22:34 -0700 (PDT) >> From: Rene J Buesa >> Subject: Re: [Histonet] Minimum sample size for breast markers >> To: Martha Ward-Pathology , >> "histonet@lists.utsouthwestern.edu" >> >> Message-ID: >> <1378992154.20733.YahooMailNeo@web163104.mail.bf1.yahoo.com> >> Content-Type: text/plain; charset=iso-8859-1 >> >> I do not think this is the adequate forum to ask this question because you may get a wrong answer that could determine your decision. >> Your pathologist is the one who should decide about this issue. >> Ren? J. >> >> >> ________________________________ >> From: Martha Ward-Pathology >> To: "histonet@lists.utsouthwestern.edu" >> Sent: Thursday, September 12, 2013 9:06 AM >> Subject: [Histonet] Minimum sample size for breast markers >> >> >> >> >> Is there a minimum number of cells that should be present when testing for ER, PR and Her2? I have looked on the CAP website but cannot see anything about this. We are constantly getting these small biopsies or FNAs for testing and I sometimes wonder what the lowest threshold is for testing...20 tumor cells, 50 tumor cells???? Any information would be appreciated. Thanks in advance for your help. >> >> Martha Ward, MT (ASCP) QIHC >> Manager >> >> Molecular Diagnostics Lab >> Medical Center Boulevard \ Winston-Salem, NC 27157 >> p 336.716.2109 \ f 336.716.5890 >> mward@wakehealth.edu > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 118, Issue 24 > ***************************************** From mikael.niku <@t> helsinki.fi Fri Sep 13 01:41:26 2013 From: mikael.niku <@t> helsinki.fi (Mikael Niku) Date: Fri Sep 13 01:41:38 2013 Subject: [Histonet] Microscope camera with good LIVE image (for teaching)? Message-ID: <5232B396.208@helsinki.fi> Hello! I'm looking for a digital camera for our histology teaching lab (for showing and explaining the slides on the screen). Essentially, a fast live image with good colours and reasonable resolution are needed. Plus a simple, easy to use software of course. And preferably not TERRIBLY expensive. It seems it's still a bit difficult to find such a solution. Any ideas? With best regards, Mikael Niku University of Helsinki, Finland From thigginsht <@t> msn.com Fri Sep 13 09:16:31 2013 From: thigginsht <@t> msn.com (Tim Higgins) Date: Fri Sep 13 09:16:36 2013 Subject: [Histonet] Dimwits Message-ID: Hello Annieinarabia, You are quite the advocate for Sir Rene J. of the most respected voices on this forum. Truly if you want to be respected, give respect and do not degrade other as he does from time to time (in my opinion). Give substance in your responses and not comment using some pompous response to a valid question. Like I said to someone off line, its not that he doesn't have knowledge (so I here, don't know him personally) to answer with useful creditable answers just at time he doesn't. I am sure everyone appreciates his sharing of knowledge, just be constructive in your responses. I don't comment on a subject unless I have useful information to share, never to belittle the person asking who is in search of a solution to a problem. Take that approach and less people will be annoyed with the responses. FYI, there is only one dimwit attached to this response unless your suggesting everyone on Histonet are dimwits. Also, not sure why you are attacking the "youngsters" on this forum, this forum is for the young, old and middle-aged. Have a fantastic Friday!!!! TiminTexas not Tim M. (Don't want anyone mad at him on accident) Message: 13 Date: Fri, 13 Sep 2013 09:52:45 +0400 From: Anne Subject: [Histonet] Re: Histonet Digest, Vol 118, Issue 24 To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=utf-8 My goodness ignorance abounds...Rene J is one of the most respected voices on this forum. And 'she' is actually a 'he' you dimwits. I would take his advice above almost anyone on this forum. You youngsters should try to emulate rather than denigrate. Rene you rock!!! Annieinarabia From PAMarcum <@t> uams.edu Fri Sep 13 09:31:33 2013 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Fri Sep 13 09:31:40 2013 Subject: [Histonet] Dimwits In-Reply-To: References: Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA32D3158ECD@Mail2Node2.ad.uams.edu> Thank you and well said!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tim Higgins Sent: Friday, September 13, 2013 9:17 AM To: annigyg@gmail.com; histonet@lists.utsouthwestern.edu Subject: [Histonet] Dimwits Hello Annieinarabia, You are quite the advocate for Sir Rene J. of the most respected voices on this forum. Truly if you want to be respected, give respect and do not degrade other as he does from time to time (in my opinion). Give substance in your responses and not comment using some pompous response to a valid question. Like I said to someone off line, its not that he doesn't have knowledge (so I here, don't know him personally) to answer with useful creditable answers just at time he doesn't. I am sure everyone appreciates his sharing of knowledge, just be constructive in your responses. I don't comment on a subject unless I have useful information to share, never to belittle the person asking who is in search of a solution to a problem. Take that approach and less people will be annoyed with the responses. FYI, there is only one dimwit attached to this response unless your suggesting everyone on Histonet are dimwits. Also, not sure why you are attacking the "youngsters" on this forum, this forum is for the young, old and middle-aged. Have a fantastic Friday!!!! TiminTexas not Tim M. (Don't want anyone mad at him on accident) Message: 13 Date: Fri, 13 Sep 2013 09:52:45 +0400 From: Anne Subject: [Histonet] Re: Histonet Digest, Vol 118, Issue 24 To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=utf-8 My goodness ignorance abounds...Rene J is one of the most respected voices on this forum. And 'she' is actually a 'he' you dimwits. I would take his advice above almost anyone on this forum. You youngsters should try to emulate rather than denigrate. Rene you rock!!! Annieinarabia _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From drbugge <@t> gmail.com Fri Sep 13 09:45:23 2013 From: drbugge <@t> gmail.com (Dawn Bugge) Date: Fri Sep 13 09:45:27 2013 Subject: [Histonet] Pathology Software: Vital Axis Message-ID: Hello Histoworld, Does anyone have reviews for a software called Vital Axis. We are trying to pick a software for our lab that can do all histology/pathology ends of the business. Any reviews good or bad would be greatly appreciated. Thanks. -- Dawn R Bugge Seattle Histology From arguellod <@t> ihn.org Fri Sep 13 10:07:20 2013 From: arguellod <@t> ihn.org (Arguello, Daniel) Date: Fri Sep 13 10:07:25 2013 Subject: [Histonet] Microscope camera with good LIVE image (for teaching)? In-Reply-To: <5232B396.208@helsinki.fi> References: <5232B396.208@helsinki.fi> Message-ID: http://arcturuslabs.com/index.php?page=store I'd also get an Apple TV to project the image to any flat screen TV or monitor. This set-up will save you hundreds (if not thousands) compared to a microscope attachment setting. iPhone camera resolution is great. Let me know if you want me to e-mail you some sample images. Hope this helps. Sincerely, Daniel Arg?ello, BS, CT(ASCP)CM Anatomic Pathology Coordinator Inspira Medical Center Woodbury Telephone (856) 853-2030 Telephone (856) 845-0100 Ext 2808 Fax (856) 853-2183 arguellod@ihn.org WEB: http://www.inspirahealthnetwork.org/ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mikael Niku Sent: Friday, September 13, 2013 2:41 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Microscope camera with good LIVE image (for teaching)? Hello! I'm looking for a digital camera for our histology teaching lab (for showing and explaining the slides on the screen). Essentially, a fast live image with good colours and reasonable resolution are needed. Plus a simple, easy to use software of course. And preferably not TERRIBLY expensive. It seems it's still a bit difficult to find such a solution. Any ideas? With best regards, Mikael Niku University of Helsinki, Finland _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------------------------------------- The information contained in this e-mail is confidential, privileged, or otherwise protected from disclosure. It is intended only for the use of the authorized individual as indicated in the e-mail. Any unauthorized disclosure, copying, distribution or taking of any action based on the contents of this material is strictly prohibited. Review by any individual other than the intended recipient does not waive or give up the physician-patient privilege. If you have received this e-mail in error, please delete it immediately. ------------------------------------------------------------------------------------------------------------- From koellingr <@t> comcast.net Fri Sep 13 10:17:02 2013 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Fri Sep 13 10:17:24 2013 Subject: [Histonet] Dimwits and off topic In-Reply-To: <41D3A1AF6FEF0643BDC89E0516A6EA32D3158ECD@Mail2Node2.ad.uams.edu> Message-ID: <207323944.2612421.1379085422199.JavaMail.root@sz0001a.emeryville.ca.mail.comcast.net> I absolutely agree with "well said" and I know I'm going to just hate myself in the morning for doing this but I can't stop. I hope everyone (I've mentioned the book to our school board and every kid I mentor in biotechnology and tutor in math) will get "The Dumbest Generation:How the digital age stupifies young Americans and jeapordizes our future" by Mark Bauerlein, professor of English at Emory University. And although directed at the 20's crowd and under, it sure seems that same mentality is seeping over to the "oldsters". The lady who plowed into me last week walking down the hallway totally oblivious and completely immersed in her texting, had to be at least 45 years old. It used to be you had to be rude and uncivil to someones face-to face or behind their back to an individual. Now you can say anything you want, anytime at all, without thinking a bit, to the whole world and often times with anonymity. I agree with the assessment that Rene has good things to offer. 95% of the time and I file those things away. But there are many good people on the HistoNet, they need not be mentioned for people know who they are out there, but even so, not sure any of them have been or should be officially or unofficially elevated to "a most respected voice on the Net". As far as histologists (started in 1967 although moved more molecular in the 90's in graduate school) and the respect or disrespect discussions, some of the postings in last couple weeks give credibility to why there might be more disrespect than respect. Is at least some of this stuff that has come through, on a "professional" forum, much better and more higher level thinking than high schoolers tossing notes back and forth when the teachers back is turned or pulling someone aside to spread the gossip "do you know what someone told a friend, who told a friend who told me that........." I know I shouldn't but have to hit send and for all the negative e-mail I'll get, I'll read it, won't respond to it but I hope it at least evokes a thought or 2 about civility and humanity that e-mails seem to be taking away. Sent from my desktop computer. Ray in Seattle ----- Original Message ----- From: "Pamela A Marcum" To: "Tim Higgins" , annigyg@gmail.com, histonet@lists.utsouthwestern.edu Sent: Friday, September 13, 2013 7:31:33 AM Subject: RE: [Histonet] Dimwits Thank you and well said!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tim Higgins Sent: Friday, September 13, 2013 9:17 AM To: annigyg@gmail.com; histonet@lists.utsouthwestern.edu Subject: [Histonet] Dimwits Hello Annieinarabia, You are quite the advocate for Sir Rene J. of the most respected voices on this forum. Truly if you want to be respected, give respect and do not degrade other as he does from time to time (in my opinion). Give substance in your responses and not comment using some pompous response to a valid question. Like I said to someone off line, its not that he doesn't have knowledge (so I here, don't know him personally) to answer with useful creditable answers just at time he doesn't. I am sure everyone appreciates his sharing of knowledge, just be constructive in your responses. I don't comment on a subject unless I have useful information to share, never to belittle the person asking who is in search of a solution to a problem. Take that approach and less people will be annoyed with the responses. FYI, there is only one dimwit attached to this response unless your suggesting everyone on Histonet are dimwits. Also, not sure why you are attacking the "youngsters" on this forum, this forum is for the young, old and middle-aged. Have a fantastic Friday!!!! TiminTexas not Tim M. (Don't want anyone mad at him on accident) Message: 13 Date: Fri, 13 Sep 2013 09:52:45 +0400 From: Anne Subject: [Histonet] Re: Histonet Digest, Vol 118, Issue 24 To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=utf-8 My goodness ignorance abounds...Rene J is one of the most respected voices on this forum. And 'she' is actually a 'he' you dimwits. I would take his advice above almost anyone on this forum. You youngsters should try to emulate rather than denigrate. Rene you rock!!! Annieinarabia _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Ronald.Houston <@t> nationwidechildrens.org Fri Sep 13 10:31:35 2013 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Fri Sep 13 10:31:43 2013 Subject: [Histonet] Dimwits In-Reply-To: <41D3A1AF6FEF0643BDC89E0516A6EA32D3158ECD@Mail2Node2.ad.uams.edu> References: <41D3A1AF6FEF0643BDC89E0516A6EA32D3158ECD@Mail2Node2.ad.uams.edu> Message-ID: Time to log off Histonet again - stop acting like children and be the professionals you are (or should be). And you wonder why we get no respect??????? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marcum, Pamela A Sent: Friday, September 13, 2013 10:32 AM To: 'Tim Higgins'; annigyg@gmail.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Dimwits Thank you and well said!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tim Higgins Sent: Friday, September 13, 2013 9:17 AM To: annigyg@gmail.com; histonet@lists.utsouthwestern.edu Subject: [Histonet] Dimwits Hello Annieinarabia, You are quite the advocate for Sir Rene J. of the most respected voices on this forum. Truly if you want to be respected, give respect and do not degrade other as he does from time to time (in my opinion). Give substance in your responses and not comment using some pompous response to a valid question. Like I said to someone off line, its not that he doesn't have knowledge (so I here, don't know him personally) to answer with useful creditable answers just at time he doesn't. I am sure everyone appreciates his sharing of knowledge, just be constructive in your responses. I don't comment on a subject unless I have useful information to share, never to belittle the person asking who is in search of a solution to a problem. Take that approach and less people will be annoyed with the responses. FYI, there is only one dimwit attached to this response unless your suggesting everyone on Histonet are dimwits. Also, not sure why you are attacking the "youngsters" on this forum, this forum is for the young, old and middle-aged. Have a fantastic Friday!!!! TiminTexas not Tim M. (Don't want anyone mad at him on accident) Message: 13 Date: Fri, 13 Sep 2013 09:52:45 +0400 From: Anne Subject: [Histonet] Re: Histonet Digest, Vol 118, Issue 24 To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=utf-8 My goodness ignorance abounds...Rene J is one of the most respected voices on this forum. And 'she' is actually a 'he' you dimwits. I would take his advice above almost anyone on this forum. You youngsters should try to emulate rather than denigrate. Rene you rock!!! Annieinarabia _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dperez56 <@t> gmail.com Fri Sep 13 12:08:01 2013 From: dperez56 <@t> gmail.com (Doris Perez) Date: Fri Sep 13 12:08:09 2013 Subject: [Histonet] Histology jobs Message-ID: Sent from my iPhone From bhughes <@t> netsoftusa.com Fri Sep 13 12:57:29 2013 From: bhughes <@t> netsoftusa.com (bhughes@netsoftusa.com) Date: Fri Sep 13 12:57:39 2013 Subject: [Histonet] Sakura Prisma stainer/film coverslipper In-Reply-To: <08861B9CF6C7774E874635A4818AE37B0125D3213C@CHEXCMS01.one.ads.che.org> References: <08861B9CF6C7774E874635A4818AE37B0125D3213C@CHEXCMS01.one.ads.che.org> Message-ID: <20130913135729.572vc9agask88os8@hostingmail.earthlink.net> Just a thought in answering Cassie's comments related to the Symphony product. ?I am in the software business and a number of companies are now attempting to integrate the tracking system into their hardware products in an attempt to make them "appear" to be more full featured. ?If specimen or custody tracking is a factor in your decision making you need to seriously consider what you may end up with when there are other software products out there that are far superior and less costly thus allowing you to purchase the hardware based solely on its merits in the histology process. ?I can provide you with a source to the software if tracking is an interest and you will be shocked by its combination of functionality/cost value. ?To prove my point, there are a number of very large laboratories in the country using this product. ?Contact me for more info. Bill Hughes NetSoft Inc. 866-463-8763 Office - Ext. 111 On Thu, 12 Sep 2013 13:27:10 -0400, "Davis, Cassie" wrote: Hi Haley, we've been using this H & E stainer and tape coverslipper for about 5 years with pretty good results even though we do not have the piece to link the two together. The very few problems we have had are related to lack of daily maintenance which is minimal. We are in the process of "handing it down" to cytology and trying to use the new Symphony for H &E because of the tracking system option. The folks that do the microtomy are not happy because using the trays are more time consuming due to the "learning curve" (we are told), which is the trade-off. I hope this helps. > > Cassandra Davis > CDavis@che-east.org > 302-575-8095 > ________________________________________ > From: "Huggins, Haley - MRMC" > > > To: > "histonet@lists.utsouthwestern.edu" > > > Sent: Wednesday, September 11, 2013 4:46 PM > Subject: [Histonet] Equipment selection > > Hi all, > > I am trying to decide on what equipment to purchase. I have picked > the processor, microtomes, embedding centers and a few other pieces, > but now I still need to decide on the H&E Stainer and the > immunostainer. My options I have in front of me are for the H&E > stainer are Sakura Prisma stainer/film coverslipper and the Leica > ST5020-CV5030 stainer/glass coverslipper. For the Immunostainer, I am > looking at the Dako Autostainer and the Leica Bond Max. Any advice on > any of these instruments would be greatly appreciated. I am trying to > finalize my decision pretty quickly. Thank you in advance for any > input. > > > > Confidentiality Notice: > This e-mail, including any attachments is the > property of Catholic Health East and is intended > for the sole use of the intended recipient(s). It may contain > information that is privileged and > confidential. Any unauthorized review, use, > disclosure, or distribution is prohibited. If you are > not the intended recipient, please delete this message, and > reply to the sender regarding the error in a separate email. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From llewllew <@t> shaw.ca Fri Sep 13 13:29:05 2013 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Fri Sep 13 13:29:21 2013 Subject: [Histonet] Microscope camera with good LIVE image (for teaching)? In-Reply-To: <5232B396.208@helsinki.fi> References: <5232B396.208@helsinki.fi> Message-ID: <52335971.8060107@shaw.ca> I bought a 2MP digital camera from Microscopes.net for a trinocular microscope for home use and it works fine. The software displays a full screen image and cost just under $200. Provided it is not going to get very heavy use, this should do what you want. The URL is (http://www.microscopenet.com/microscope-accessories-cameras-c-89_49.html). If you want better resolution, they have cameras up to 14MP, at higher prices, of course. Bryan Llewellyn Mikael Niku wrote: > Hello! > > I'm looking for a digital camera for our histology teaching lab (for > showing and explaining the slides on the screen). > Essentially, a fast live image with good colours and reasonable > resolution are needed. Plus a simple, easy to use software of course. > And preferably not TERRIBLY expensive. > > It seems it's still a bit difficult to find such a solution. Any ideas? > > With best regards, > Mikael Niku > University of Helsinki, Finland > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From kim-lake <@t> uiowa.edu Fri Sep 13 13:40:24 2013 From: kim-lake <@t> uiowa.edu (Lake, Kim S) Date: Fri Sep 13 13:40:44 2013 Subject: [Histonet] Can I microwave Sukura racks? Message-ID: Our lab just got a new-to-us Sukura DRS 2000, and I was wondering if the racks can be microwaved. Does anyone microwave just the part that holds slides, and have you had any problem with warping? I would like to microwave a rack for 2 minutes to fully dry my slides before staining. Thanks! Kim Lake Laboratory Manager Oral Pathology Laboratory University of Iowa College of Dentistry Phone (319) 384 4433 Fax (319) 353 5569 From koellingr <@t> comcast.net Fri Sep 13 16:45:21 2013 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Fri Sep 13 16:45:54 2013 Subject: [Histonet] OT OT OT off topic histologically but still devastatingly sad In-Reply-To: <907069281.2624449.1379108218718.JavaMail.root@sz0001a.emeryville.ca.mail.comcast.net> Message-ID: <1445547596.2624640.1379108721429.JavaMail.root@sz0001a.emeryville.ca.mail.comcast.net> Just hours after I sent out my recent e-mail regarding the digital and internet conundrum and how uncivil it can tend to make humans by its indiscriminate use without first any thought, here is a headline copy/paste from the national news: Police: Girl, 12, Bullied Online Before Suicide Is the digital world and the internet the panacea and great wonder that it is purported to be? Are we really as kind and as civil and advanced as humans as we believe we are? Just some non-histologic food for thought. Ray in Seattle From rjbuesa <@t> yahoo.com Sat Sep 14 11:41:15 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Sep 14 11:43:59 2013 Subject: [Histonet] Can I microwave Sukura racks? In-Reply-To: References: Message-ID: <1379176875.16697.YahooMailNeo@web163101.mail.bf1.yahoo.com> If you refer to those brownish ones, they are made of hard plastic and can be microwaved. Remove the handles though. Ren? J. ________________________________ From: "Lake, Kim S" To: "histonet@lists.utsouthwestern.edu" Sent: Friday, September 13, 2013 2:40 PM Subject: [Histonet] Can I microwave Sukura racks? Our lab just got a new-to-us Sukura DRS 2000, and I was wondering if the racks can be microwaved.? Does anyone microwave just the part that holds slides, and have you had any problem with warping?? I would like to microwave a rack for 2 minutes to fully dry my slides before staining. Thanks! Kim Lake Laboratory Manager Oral Pathology Laboratory University of Iowa College of Dentistry Phone (319) 384 4433 Fax (319) 353 5569 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joewalker <@t> rrmc.org Sat Sep 14 13:44:39 2013 From: joewalker <@t> rrmc.org (Joe W. Walker, Jr.) Date: Sat Sep 14 13:44:50 2013 Subject: [Histonet] RE: ThinPrep In-Reply-To: <2B2ECF33934F5D4996D8BE03EFDF397607D8A092@VHAV09MSGA3.v09.med.va.gov> References: <2B2ECF33934F5D4996D8BE03EFDF397607D8A092@VHAV09MSGA3.v09.med.va.gov> Message-ID: <3C2378778400AD448ADA6FD6BDB7CCCC18077B5D@RRMBX03.rrmc.local> Hi Lisa, I am not aware of a specific CAP regulation/question related to the validation of the ThinPrep processor. However, there are general questions related to ensuring your devices are functioning as intended prior to using them on patient specimens. This should have been done when the device was initially setup in the lab and with the help of the manufacturer. They should help you verify that the instrument is working as designed. Joe W. Walker, Jr. MS, SCT(ASCP)CM Anatomical Pathology Manager Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P: 802.747.1790 F: 802.747.6525 Email joewalker@rrmc.org www.rrmc.org Our Vision: To be the Best Community Healthcare System in New England Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet Recognition? and the Governor's Award for Performance Excellence -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of White, Lisa M. Sent: Thursday, September 12, 2013 9:16 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ThinPrep Does anyone know a CAP regulation for validation of ThinPrep? Lisa White HT(ASCP) Supervisory HT James H. Quillen VAMC Corner of Veterans Way and Lamont VAMC Warehouse BLDG. 205 PO Box 4000 PLMS 113 Mountain Home, TN 37684 423-979-3567 423-979-3401 fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. Thank You From debgranato <@t> yahoo.com Mon Sep 16 10:29:29 2013 From: debgranato <@t> yahoo.com (Debbie Granato) Date: Mon Sep 16 10:32:13 2013 Subject: [Histonet] Urine Cytology Message-ID: <1379345369.7648.YahooMailNeo@web161205.mail.bf1.yahoo.com> Good Morning! ? Can anyone please tell me the longest that a fixed urine cytology can stay in the refrigerator before processing. ? ?I appreciate your help and input! ? Debbie Granato HT(ASCP) From MYang <@t> emc.org Mon Sep 16 11:30:52 2013 From: MYang <@t> emc.org (Yang, Mari) Date: Mon Sep 16 11:31:05 2013 Subject: [Histonet] RE: ThinPrep In-Reply-To: <3C2378778400AD448ADA6FD6BDB7CCCC18077B5D@RRMBX03.rrmc.local> References: <2B2ECF33934F5D4996D8BE03EFDF397607D8A092@VHAV09MSGA3.v09.med.va.gov> <3C2378778400AD448ADA6FD6BDB7CCCC18077B5D@RRMBX03.rrmc.local> Message-ID: <5F4186AA4871E74CB729E1B43BAAF7310417B2EB@NT269.info.sys> Lisa, If the ThinPrep processor is new to your organization, then the requirements below may apply. CYP.05257 Implementation/Verification Protocol Phase II There is documentation of adherence to the manufacturer's recommended protocol(s) for implementation and verification of new instruments. NOTE: Before implementing use of new gynecologic liquid-based methods and instruments, automated preparations, and automated screening instruments, the laboratory must verify and document the functioning of the instrument in its own specific laboratory environment, including the capability of the instrument to replace existing procedure(s), if applicable. If the manufacturer does not provide verification and instrument monitoring recommendations, the laboratory must document the specific verification procedure used. Evidence of Compliance: ? Records of completed instrument/method verification consistent with manufacturer's recommendations OR records of an alternative documented verification procedure approved by the section director REFERENCES 1) Chang AR, et al. Can technology expedite the cervical cancer screening process? A Hong Kong experience using the AutoPap Primary Screening System with location-guided screening capability. Am J Clin Pathol. 2002; 117:399-437-443 2) Wilbur DC, et al. Location-guided screening of liquid-based cervical cytology specimens. A potential improvement in accuracy and productivity is demonstrated in a preclinical feasibility trial. Am J Clin Pathol. 2002; 118:399-407 3) Department of Health and Human Services, Centers for Medicare and Medicaid Services. Clinical laboratory improvement amendments of 1988; final rule. Fed Register. 2003(Jan 24):3707 [42 CFR493.1253, 493.1255, and 493.1274(g)] Good Luck! Mari Mari Yang, MHA, CT(ASCP)CMHTLCM Cytology Supervisor Eisenhower Medical Center Tel: 760.773.2009 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe W. Walker, Jr. Sent: Saturday, September 14, 2013 11:45 AM To: White, Lisa M.; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: ThinPrep Hi Lisa, I am not aware of a specific CAP regulation/question related to the validation of the ThinPrep processor. However, there are general questions related to ensuring your devices are functioning as intended prior to using them on patient specimens. This should have been done when the device was initially setup in the lab and with the help of the manufacturer. They should help you verify that the instrument is working as designed. Joe W. Walker, Jr. MS, SCT(ASCP)CM Anatomical Pathology Manager Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P: 802.747.1790 F: 802.747.6525 Email joewalker@rrmc.org www.rrmc.org Our Vision: To be the Best Community Healthcare System in New England Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet Recognition? and the Governor's Award for Performance Excellence -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of White, Lisa M. Sent: Thursday, September 12, 2013 9:16 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ThinPrep Does anyone know a CAP regulation for validation of ThinPrep? Lisa White HT(ASCP) Supervisory HT James H. Quillen VAMC Corner of Veterans Way and Lamont VAMC Warehouse BLDG. 205 PO Box 4000 PLMS 113 Mountain Home, TN 37684 423-979-3567 423-979-3401 fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. Thank You _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From chesarato <@t> hotmail.com Mon Sep 16 12:17:56 2013 From: chesarato <@t> hotmail.com (Cesar Francisco Romero) Date: Mon Sep 16 12:18:05 2013 Subject: [Histonet] Myc Protein Antibody and Protocol Message-ID: Dear People from Histonet I need to know if somebody is using Anti Myc Protein Antibody in the Panel for Large Cell Lymphomas. I will appreciate any advice on the brand of the antibody and the protocol to make it work. I do not use any Automated Stainer, only Manual Immunohistochemistry. Thank You All in advance. Sincerely yours Cesar Romero Buenos Aires Argentina From Klaus.Kruttwig <@t> ucsf.edu Mon Sep 16 12:32:27 2013 From: Klaus.Kruttwig <@t> ucsf.edu (Kruttwig, Klaus) Date: Mon Sep 16 12:37:10 2013 Subject: [Histonet] Myc Protein Antibody and Protocol In-Reply-To: References: Message-ID: Hi Cesar, I am using a anti-Myc antibody for immunohistochemistry on paraffin and frozen sections of mouse tumors and I know from co-workers that it works on other tumors of human origin very well, at least for Western Blotting. The antibody is from Epitomics (Cat# 1472-1) and it is really great. I use it for paraffin IHC in a 1:500 to 1:1000 dilution. Thanks, Klaus Klaus Kruttwig Department of Cell and Tissue Biology University of Californa - San Francisco San Francisco, CA, USA ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Cesar Francisco Romero [chesarato@hotmail.com] Sent: Monday, September 16, 2013 10:17 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Myc Protein Antibody and Protocol Dear People from Histonet I need to know if somebody is using Anti Myc Protein Antibody in the Panel for Large Cell Lymphomas. I will appreciate any advice on the brand of the antibody and the protocol to make it work. I do not use any Automated Stainer, only Manual Immunohistochemistry. Thank You All in advance. Sincerely yours Cesar Romero Buenos Aires Argentina _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jorourke <@t> allied360.com Mon Sep 16 12:50:30 2013 From: jorourke <@t> allied360.com (Judy O'Rourke) Date: Mon Sep 16 12:50:43 2013 Subject: [Histonet] Article: Anatomic Pathology Takes a Leap in Quality Process Improvement Message-ID: William DeSalvo, BS HTL(ASCP), has written the cover article in CLP's September issue: Anatomic Pathology Takes a Leap in Quality Process Improvement Found here in the digital edition (please flip to page 8): http://edition.pagesuite-professional.co.uk//launch.aspx?pbid=db6b2441-3876-40c8-8eb3-8a480a616c4e Regards, Judy O'Rourke Editor/CLP From cjohnson <@t> nmda.nmsu.edu Mon Sep 16 15:52:59 2013 From: cjohnson <@t> nmda.nmsu.edu (Johnson, Carole) Date: Mon Sep 16 15:53:13 2013 Subject: [Histonet] Amyloid Precursor Protein (APP) by IHC Message-ID: Hello All, Does anyone out there in Histoland have experience with this antibody? I have been asked to look into running IHC on pig brain on the Bond Max platform. I am looking into the antibodies from LS Bio and Invitrogen/LifeSciences, and would appreciate any insight you could provide. Thanks in advance for your help! If you prefer, you may reply privately at the email below. Carole Johnson, HT(ASCP)cm Histopathology Section Supervisor New Mexico Department of Agriculture Veterinary Diagnostic Services cjohnson@nmda.nmsu.edu Confidentiality Notice: New Mexico has a very broad public records law. Most written communications to or from state employees are public records. Your e-mail communications may therefore be subject to public disclosure. This e-mail, including all attachments is for the sole use of the intended recipients. Any unauthorized review, use, disclosure or distribution is prohibited unless specifically provided under the New Mexico Inspection of Public Records Act. From mbmphoto <@t> gmail.com Mon Sep 16 22:35:01 2013 From: mbmphoto <@t> gmail.com (Maria Mejia) Date: Mon Sep 16 22:35:14 2013 Subject: [Histonet] searching for miles tissue tech II vacuum paraffin infiltator Message-ID: <397AA33D-0C12-4D28-8A9B-810F4A3744A9@gmail.com> I'm searching for a Miles tissue tek II vacuum paraffin infiltrator that doesn't cost an arm & a leg! I'm hoping someone out there has one for a reasonable price! Please let me hear from you. Regards Maria Mejia San Francisco, CA 94103 From lpwenk <@t> sbcglobal.net Tue Sep 17 05:37:32 2013 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Tue Sep 17 05:37:48 2013 Subject: [Histonet] Unsubscribe for NSH Symposium Message-ID: <652A8C1276A7440584DFC95F344D03A1@HP2010> For those going to the NSH Symposium, PLEASE unsubscribe, rather than having everyone on the email list read your return message of ?I am out of the office? for every single Histonet email sent to you. Reminder on how: - Go to the bottom on any Histonet email - Click on the internet link (the one that starts http://) - Scroll to the bottom, fill in the information in the last ?rectangle? Peggy A. Wenk, HTL(ASCP)SLS From caspake <@t> yahoo.com Tue Sep 17 08:58:33 2013 From: caspake <@t> yahoo.com (Cindy) Date: Tue Sep 17 08:58:45 2013 Subject: [Histonet] Nail specimens Message-ID: <290D1B1B-B4D2-4628-A555-D13664C68CD7@yahoo.com> Does anyone have a procedure for cutting nails and getting them to stay on the slide? Help please! Thanks, Cindy Sent from my iPhone From lowenjeff <@t> hotmail.com Tue Sep 17 09:27:04 2013 From: lowenjeff <@t> hotmail.com (jeff lowen) Date: Tue Sep 17 09:27:12 2013 Subject: [Histonet] Nail specimens In-Reply-To: <290D1B1B-B4D2-4628-A555-D13664C68CD7@yahoo.com> References: <290D1B1B-B4D2-4628-A555-D13664C68CD7@yahoo.com> Message-ID: Use + charged slide and microwave prior to routine drying/melting in your slide drying oven.Also, "Nair" can help soften nails for cutting. Face in, coat cut surface with it and let stand for ?(1/2 hr.), wipe off Nair, chill block and cut as routine. > From: caspake@yahoo.com > Date: Tue, 17 Sep 2013 06:58:33 -0700 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Nail specimens > > Does anyone have a procedure for cutting nails and getting them to stay on the slide? Help please! > > Thanks, > Cindy > > Sent from my iPhone > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Vickroy.Jim <@t> mhsil.com Tue Sep 17 09:36:40 2013 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Tue Sep 17 09:36:48 2013 Subject: [Histonet] Preventative Maintenance on reagent and formalin recyclers Message-ID: I am wondering if people have a pm annually or are some stretching it out to do a pm every two years? Thanks James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From rjbuesa <@t> yahoo.com Tue Sep 17 09:49:16 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Sep 17 09:49:24 2013 Subject: [Histonet] Nail specimens In-Reply-To: <290D1B1B-B4D2-4628-A555-D13664C68CD7@yahoo.com> References: <290D1B1B-B4D2-4628-A555-D13664C68CD7@yahoo.com> Message-ID: <1379429356.28358.YahooMailNeo@web163105.mail.bf1.yahoo.com> Cindy: Nails need special treatment: 1- fix the nails for 24 hours 2- place them in a 10% aq. sol. of either sodium hydroxide or potassium hydroxide until when you touch them they are flexible. Wash them in water before you touch them.?Softening the keratin?in the nail will take about 30-45 minutes. 3- wash them in running water until when you touch them your fingers do not have a "soapy" feeling. 4- process and embed as usual 5- use (+) charged slides for the sections 6- heat the sections for 5-10 nim. at 60?C in an oven before staining. If you want I can send you a more detailed procedure. Ren? J. ________________________________ From: Cindy To: "histonet@lists.utsouthwestern.edu" Sent: Tuesday, September 17, 2013 9:58 AM Subject: [Histonet] Nail specimens Does anyone have a procedure for cutting nails and getting them to stay on the slide? Help please! Thanks, Cindy Sent from my iPhone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amurvosh <@t> advancederm.net Tue Sep 17 10:23:44 2013 From: amurvosh <@t> advancederm.net (Anne Murvosh) Date: Tue Sep 17 10:23:57 2013 Subject: [Histonet] Flammable Liquid Message-ID: <4AD6A4E531E8C943A730559B6B81DF07C0D210@dc.Advancederm.net> Can anyone tell me how many gallons of Flammable liquid can be stored in a space per CLIA regulations. There website is useless. Thanks Anne From cheastys <@t> svm.vetmed.wisc.edu Tue Sep 17 10:26:13 2013 From: cheastys <@t> svm.vetmed.wisc.edu (Sandra Cheasty) Date: Tue Sep 17 10:26:16 2013 Subject: [Histonet] Processing Problem Message-ID: Hi All, I need help with two issues. I have a researcher who is sending us porcine skin, about 2mm x 2mm x 1mm tall. Issue 1: There is a lesion in the center, and although he wants the skin sectioned through the lesion eventually, he says if he bisects the chunk of skin before fixation, the lesion becomes distorted. So, he is fixing them in the 2x2x1 chunks, and the 10% formalin (and subsequent processing reagents) are not penetrating. Does anyone know of either a more penetrating fixative or a less distorting one so we can bisect the skin before fixation? Issue 2: Even on smaller sections that fixed and processed well, we are having issues with the porcine skin sections staying on the slides. We use Superfrost Plus slides, drain them, air dry them overnight, and then they go on the "Extra Oven" program on the stainer. (25 minutes in the oven.) Any suggestions on other slides, drying techniques to try? My background is that of a certified Histologist for 30 years, with experience in many labs in various parts of the country. This research project has me stymied! Thanks for your help! Sandy UW Madison School of Veterinary Medicine From BDeBrosse-Serra <@t> isisph.com Tue Sep 17 10:46:11 2013 From: BDeBrosse-Serra <@t> isisph.com (Bea DeBrosse-Serra) Date: Tue Sep 17 10:46:25 2013 Subject: [Histonet] Preventative Maintenance on reagent and formalin recyclers In-Reply-To: References: Message-ID: Annually. Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Tuesday, September 17, 2013 7:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Preventative Maintenance on reagent and formalin recyclers I am wondering if people have a pm annually or are some stretching it out to do a pm every two years? Thanks James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Sep 17 10:47:21 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Sep 17 10:47:29 2013 Subject: [Histonet] Processing Problem In-Reply-To: References: Message-ID: <1379432841.47247.YahooMailNeo@web163101.mail.bf1.yahoo.com> A sample 2mmx2mmx1mm is quite small and there should not be any problems with either fixation of processing but there is where the problem my reside. 1- fixing in 10% formalin: is it neutral buffered? For such a small piece 24 hours will be enough but to be absolutely sure try leaving the pieces for 48 hours. 2- sectioning should not be a problem but I advise that you check the times in the reagents. Such small pieces should be less time in the alcohols. Since it is pig skin try using more time in the "ante-medium". What are you using, xylene? And give extra time in the infiltration steps (melted paraffin wax). With these precautions you should have no problems sectioning the samples. Ren? J. ________________________________ From: Sandra Cheasty To: "histonet@lists.utsouthwestern.edu" Cc: Sandra Cheasty Sent: Tuesday, September 17, 2013 11:26 AM Subject: [Histonet] Processing Problem Hi All, ? ? ? ? ? ? ? ? I need help with two issues. I have a researcher who is sending us porcine skin, about 2mm x 2mm x 1mm tall. Issue 1: There is a lesion in the center, and although he wants the skin sectioned through the lesion eventually, he says if he bisects the chunk of skin before fixation, the lesion becomes distorted. So, he is fixing them in the 2x2x1 chunks, and the 10% formalin (and subsequent processing reagents) are not penetrating. Does anyone know of either a more penetrating fixative or a less distorting one so we can bisect the skin before fixation? Issue 2: Even on smaller sections that fixed and processed well, we are having issues with the porcine skin sections staying on the slides. We use Superfrost Plus slides, drain them, air dry them overnight, and then they go on the "Extra Oven" program on the stainer. (25 minutes in the oven.) Any suggestions on other slides, drying techniques to try? My background is that of a certified Histologist for 30 years, with experience in many labs in various parts of the country. This research project has me stymied! Thanks for your help! Sandy UW Madison School of Veterinary Medicine _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Tue Sep 17 10:49:57 2013 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Tue Sep 17 10:49:56 2013 Subject: [Histonet] RE: Processing Problem In-Reply-To: References: Message-ID: <14E2C6176416974295479C64A11CB9AE019BB56FE12F@SBS2K8.premierlab.local> Sandra Sandra We section large porcine skin samples all of the time. When there are lesions present we normally like to bisect after fixation, that way we get a nice even edge to section. You want to bisect slightly uneven (1mm) so once you trim in you are at the center of the lesion. It's very important that you try to capture sections that are at the center of the lesion, so great care must be taken in grossing, embedding and trimming in samples like these. These samples heal from the outside in so if sectioning is not consistently in the center you can bias/skew the results. Are you sure that that samples are 2mm x 2mm x 1mm or did you mean to say 2cm x 2 cm x 1cm that makes more sense to me. These samples will need to remain in fixative for at least 24 hours prior to you bisecting and trimming, after you bisect them I would continue to fix for an additional 24 to 48 hours. If the lesion is a punch it will be about 8 mm in diameter, your trimmed piece of tissue should be 2cm x 5-6mm x 1cm (LxWxD). We process these types of samples on a longer processing cycle. 1.5 to 2 hours per station with additional absolute and xylenes steps so there are 3 absolute and 3 xylenes and 4 paraffins . If they are processed well they should stay on the slide when they are sectioned when using a good plus slide. Good Luck Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Laboratory Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 Work (303) 682-3949 Fax (303) 682-9060 Cell (303) 881-0763 liz@premierlab.com www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sandra Cheasty Sent: Tuesday, September 17, 2013 9:26 AM To: histonet@lists.utsouthwestern.edu Cc: Sandra Cheasty Subject: [Histonet] Processing Problem Hi All, I need help with two issues. I have a researcher who is sending us porcine skin, about 2mm x 2mm x 1mm tall. Issue 1: There is a lesion in the center, and although he wants the skin sectioned through the lesion eventually, he says if he bisects the chunk of skin before fixation, the lesion becomes distorted. So, he is fixing them in the 2x2x1 chunks, and the 10% formalin (and subsequent processing reagents) are not penetrating. Does anyone know of either a more penetrating fixative or a less distorting one so we can bisect the skin before fixation? Issue 2: Even on smaller sections that fixed and processed well, we are having issues with the porcine skin sections staying on the slides. We use Superfrost Plus slides, drain them, air dry them overnight, and then they go on the "Extra Oven" program on the stainer. (25 minutes in the oven.) Any suggestions on other slides, drying techniques to try? My background is that of a certified Histologist for 30 years, with experience in many labs in various parts of the country. This research project has me stymied! Thanks for your help! Sandy UW Madison School of Veterinary Medicine _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Sep 17 10:56:27 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Sep 17 10:56:32 2013 Subject: [Histonet] Flammable Liquid In-Reply-To: <4AD6A4E531E8C943A730559B6B81DF07C0D210@dc.Advancederm.net> References: <4AD6A4E531E8C943A730559B6B81DF07C0D210@dc.Advancederm.net> Message-ID: <1379433387.36830.YahooMailNeo@web163104.mail.bf1.yahoo.com> The standard is 1 gallon per every 24 square feet of lab area, so if your lab has 240 square feet surface, you can store 10 gals of flammable liquids. Ren? J. ________________________________ From: Anne Murvosh To: histonet@lists.utsouthwestern.edu Sent: Tuesday, September 17, 2013 11:23 AM Subject: [Histonet] Flammable Liquid Can anyone tell me how many gallons of Flammable liquid can be stored in a space per CLIA regulations.? There website is useless.? Thanks Anne _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From shive003 <@t> umn.edu Tue Sep 17 11:07:00 2013 From: shive003 <@t> umn.edu (Jan Shivers) Date: Tue Sep 17 11:07:06 2013 Subject: [Histonet] Amyloid Precursor Protein (APP) by IHC In-Reply-To: References: Message-ID: Carole, This may or may not be of help - Millipore's xAPP (MAB348) works on horses; HIER using high pH retrieval solution. I don't have data on reactivity on pigs yet. Good luck, Jan Shivers UMN Vet Diag Lab St. Paul, MN On Mon, Sep 16, 2013 at 3:52 PM, Johnson, Carole wrote: > Hello All, > > Does anyone out there in Histoland have experience with this antibody? I > have been asked to look into running IHC on pig brain on the Bond Max > platform. I am looking into the antibodies from LS Bio and > Invitrogen/LifeSciences, and would appreciate any insight you could > provide. Thanks in advance for your help! If you prefer, you may reply > privately at the email below. > > Carole Johnson, HT(ASCP)cm > Histopathology Section Supervisor > New Mexico Department of Agriculture > Veterinary Diagnostic Services > > cjohnson@nmda.nmsu.edu > > > > > Confidentiality Notice: New Mexico has a very broad public records law. > Most written communications to or from state employees are public records. > Your e-mail communications may therefore be subject to public disclosure. > This e-mail, including all attachments is for the sole use of the intended > recipients. Any unauthorized review, use, disclosure or distribution is > prohibited unless specifically provided under the New Mexico Inspection of > Public Records Act. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From trathborne <@t> somerset-healthcare.com Tue Sep 17 11:14:48 2013 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Tue Sep 17 11:15:36 2013 Subject: [Histonet] Flammable Liquid In-Reply-To: <1379433387.36830.YahooMailNeo@web163104.mail.bf1.yahoo.com> References: <4AD6A4E531E8C943A730559B6B81DF07C0D210@dc.Advancederm.net> <1379433387.36830.YahooMailNeo@web163104.mail.bf1.yahoo.com> Message-ID: <3AD061FE740D464FAC7BF6B5CFB75707A8A687CE@smcmail02.somerset-healthcare.com> Is this on the CLIA website? I agree that it's difficult to find anything on there. If you're able to provide a direct link that would be great! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, September 17, 2013 11:56 AM To: Anne Murvosh; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Flammable Liquid The standard is 1 gallon per every 24 square feet of lab area, so if your lab has 240 square feet surface, you can store 10 gals of flammable liquids. Ren? J. ________________________________ From: Anne Murvosh To: histonet@lists.utsouthwestern.edu Sent: Tuesday, September 17, 2013 11:23 AM Subject: [Histonet] Flammable Liquid Can anyone tell me how many gallons of Flammable liquid can be stored in a space per CLIA regulations.? There website is useless.? Thanks Anne _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Tue Sep 17 11:21:39 2013 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Tue Sep 17 11:21:37 2013 Subject: [Histonet] Preventative Maintenance on reagent and formalin recyclers In-Reply-To: References: Message-ID: <14E2C6176416974295479C64A11CB9AE019BB56FE134@SBS2K8.premierlab.local> James We PM and calibrate annually, except for one piece of equipment - we PM the recycler every two years. We are GLP compliant and not clinical. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Laboratory Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 Work (303) 682-3949 Fax (303) 682-9060 Cell (303) 881-0763 liz@premierlab.com www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Tuesday, September 17, 2013 8:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Preventative Maintenance on reagent and formalin recyclers I am wondering if people have a pm annually or are some stretching it out to do a pm every two years? Thanks James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wbenton <@t> cua.md Tue Sep 17 11:21:35 2013 From: wbenton <@t> cua.md (Walter Benton) Date: Tue Sep 17 11:21:50 2013 Subject: [Histonet] Flammable Liquid In-Reply-To: <3AD061FE740D464FAC7BF6B5CFB75707A8A687CE@smcmail02.somerset-healthcare.com> References: <4AD6A4E531E8C943A730559B6B81DF07C0D210@dc.Advancederm.net> <1379433387.36830.YahooMailNeo@web163104.mail.bf1.yahoo.com>, <3AD061FE740D464FAC7BF6B5CFB75707A8A687CE@smcmail02.somerset-healthcare.com> Message-ID: <0B8979A204680A42B93A52B486088CD93900420788@CUAEXH1.GCU-MD.local> https://www.osha.gov/dte/library/flammable_liquids/flammable_liquids.html Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) ChesapeakeUrology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni [trathborne@somerset-healthcare.com] Sent: Tuesday, September 17, 2013 12:14 PM To: 'Rene J Buesa'; Anne Murvosh; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Flammable Liquid Is this on the CLIA website? I agree that it's difficult to find anything on there. If you're able to provide a direct link that would be great! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, September 17, 2013 11:56 AM To: Anne Murvosh; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Flammable Liquid The standard is 1 gallon per every 24 square feet of lab area, so if your lab has 240 square feet surface, you can store 10 gals of flammable liquids. Ren? J. ________________________________ From: Anne Murvosh To: histonet@lists.utsouthwestern.edu Sent: Tuesday, September 17, 2013 11:23 AM Subject: [Histonet] Flammable Liquid Can anyone tell me how many gallons of Flammable liquid can be stored in a space per CLIA regulations. There website is useless. Thanks Anne _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From lcolbert <@t> pathmdlabs.com Tue Sep 17 11:45:04 2013 From: lcolbert <@t> pathmdlabs.com (Laurie Colbert) Date: Tue Sep 17 11:48:05 2013 Subject: [Histonet] Nail specimens In-Reply-To: <290D1B1B-B4D2-4628-A555-D13664C68CD7@yahoo.com> References: <290D1B1B-B4D2-4628-A555-D13664C68CD7@yahoo.com> Message-ID: <12ECD7346266D74691EC2BFC75285E452F340358@BFL323E10.pathmdlabs.local> We purchase a solution called "Nail Prep" from Stat Lab. It is wonderful!! We had a horrible time with nails before trying this. Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cindy Sent: Tuesday, September 17, 2013 6:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Nail specimens Does anyone have a procedure for cutting nails and getting them to stay on the slide? Help please! Thanks, Cindy Sent from my iPhone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Erin.Martin <@t> ucsf.edu Tue Sep 17 12:39:08 2013 From: Erin.Martin <@t> ucsf.edu (Martin, Erin) Date: Tue Sep 17 12:39:24 2013 Subject: [Histonet] Cutting Nails Message-ID: <24B7B291CC88D04AB663958E77A1F59D181B48@ex09.net.ucsf.edu> We use a 50% solution of Nair in water to soak nail blocks after we have faced into them. Then albumin to keep them on the slides. Works great! Erin Martin, Histology Supervisor UCSF Dermatopathology Service 415-353-7248 Confidentiality Notice The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential and/or priviledged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited. If you receive this in error, please contact the sender and delete the material from any computer. From lowenjeff <@t> hotmail.com Tue Sep 17 13:08:08 2013 From: lowenjeff <@t> hotmail.com (jeff lowen) Date: Tue Sep 17 13:08:13 2013 Subject: [Histonet] Cutting Nails In-Reply-To: <24B7B291CC88D04AB663958E77A1F59D181B48@ex09.net.ucsf.edu> References: <24B7B291CC88D04AB663958E77A1F59D181B48@ex09.net.ucsf.edu> Message-ID: Thanks, and I defer all future nail issues to you...lol...ditto all other skin. > From: Erin.Martin@ucsf.edu > To: histonet@lists.utsouthwestern.edu > Date: Tue, 17 Sep 2013 17:39:08 +0000 > Subject: [Histonet] Cutting Nails > > We use a 50% solution of Nair in water to soak nail blocks after we have faced into them. Then albumin to keep them on the slides. Works great! > > > > Erin Martin, Histology Supervisor > UCSF Dermatopathology Service > 415-353-7248 > > Confidentiality Notice > The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential and/or priviledged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited. If you receive this in error, please contact the sender and delete the material from any computer. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lcolbert <@t> pathmdlabs.com Tue Sep 17 14:27:56 2013 From: lcolbert <@t> pathmdlabs.com (Laurie Colbert) Date: Tue Sep 17 14:31:00 2013 Subject: [Histonet] Punch Biopsies Message-ID: <12ECD7346266D74691EC2BFC75285E452F340418@BFL323E10.pathmdlabs.local> We are having trouble cutting punch bx's. Depending on their size, they are either left whole, bisected, or trisected. We process them on a 9 hour run with other big tissue (skin excisions, tonsils, nasal tissue, breast tissue, etc). Most of the time, the punches seemed to not be processed well - they are hard to section and we get incomplete sections. For the most part, all of the other tissue is fine to cut. Is there a trick to cutting/processing punches? We have never had so much trouble with them! Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD From rjbuesa <@t> yahoo.com Tue Sep 17 14:58:17 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Sep 17 14:58:31 2013 Subject: [Histonet] Punch Biopsies In-Reply-To: <12ECD7346266D74691EC2BFC75285E452F340418@BFL323E10.pathmdlabs.local> References: <12ECD7346266D74691EC2BFC75285E452F340418@BFL323E10.pathmdlabs.local> Message-ID: <1379447897.3594.YahooMailNeo@web163104.mail.bf1.yahoo.com> Regardless of the size I always bisected punch biopsies and then embedded them side-by-side resting on their flat surfaces. You have to realize that otherwise you were going to lose half the biopsy?during the trimming step. Also, if the biopsy is bisected processing is facilitated. Place the skin stratum facing the blade, if the dermis is in front of the blade, the skin will be teared off. Ren? J. ________________________________ From: Laurie Colbert To: "Histonet Post (histonet@lists.utsouthwestern.edu)" Sent: Tuesday, September 17, 2013 3:27 PM Subject: [Histonet] Punch Biopsies We are having trouble cutting punch bx's.? Depending on their size, they are either left whole, bisected, or trisected.? We process them on a 9 hour run with other big tissue (skin excisions, tonsils, nasal tissue, breast tissue, etc).? Most of the time, the punches seemed to not be processed well - they are hard to section and we get incomplete sections.? For the most part, all of the other tissue is fine to cut.? Is there a trick to cutting/processing punches? We have never had so much trouble with them! Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wgray19 <@t> sc.rr.com Tue Sep 17 17:15:32 2013 From: wgray19 <@t> sc.rr.com (Wanda Shotsberger AGray) Date: Tue Sep 17 17:15:39 2013 Subject: [Histonet] Re: Nail specimens Message-ID: <1412dfcb470.277c.145c840c2545cc8d226fb8e47258f83d@sc.rr.com> From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cindy Sent: Tuesday, September 17, 2013 6:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Nail specimens Does anyone have a procedure for cutting nails and getting them to stay on the slide? Help please! Thanks, Cindy Cindy, We soak faced nail blocks in 1N sodium hydroxide for about 20 mins, then section @ 5um (pathologist preference), mount on charged slides and dry @ 65?C overnight. Cut beautifully and have very few wash offs, even after DPAS staining. Good luck! Wanda Shotsberger HT/HTL Sent with AquaMail for Android http://www.aqua-mail.com From lowenjeff <@t> hotmail.com Tue Sep 17 17:33:03 2013 From: lowenjeff <@t> hotmail.com (jeff lowen) Date: Tue Sep 17 17:33:09 2013 Subject: [Histonet] Nail specimens In-Reply-To: References: <290D1B1B-B4D2-4628-A555-D13664C68CD7@yahoo.com>, Message-ID: with all the answers, r u sorry u asked the question?.....lol From: lowenjeff@hotmail.com To: caspake@yahoo.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Nail specimens Date: Tue, 17 Sep 2013 07:27:04 -0700 Use + charged slide and microwave prior to routine drying/melting in your slide drying oven.Also, "Nair" can help soften nails for cutting. Face in, coat cut surface with it and let stand for ?(1/2 hr.), wipe off Nair, chill block and cut as routine. > From: caspake@yahoo.com > Date: Tue, 17 Sep 2013 06:58:33 -0700 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Nail specimens > > Does anyone have a procedure for cutting nails and getting them to stay on the slide? Help please! > > Thanks, > Cindy > > Sent from my iPhone > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lowenjeff <@t> hotmail.com Tue Sep 17 17:44:10 2013 From: lowenjeff <@t> hotmail.com (jeff lowen) Date: Tue Sep 17 17:44:14 2013 Subject: [Histonet] Nail specimens In-Reply-To: References: <290D1B1B-B4D2-4628-A555-D13664C68CD7@yahoo.com>, , , Message-ID: and one more thing, go with the answer from the guy who works in Dermpath at UCSF, if he doesn't know, well ? > From: lowenjeff@hotmail.com > To: caspake@yahoo.com; histonet@lists.utsouthwestern.edu > Date: Tue, 17 Sep 2013 15:33:03 -0700 > Subject: RE: [Histonet] Nail specimens > CC: > > with all the answers, r u sorry u asked the question?.....lol > > From: lowenjeff@hotmail.com > To: caspake@yahoo.com; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Nail specimens > Date: Tue, 17 Sep 2013 07:27:04 -0700 > > > > > Use + charged slide and microwave prior to routine drying/melting in your slide drying oven.Also, "Nair" can help soften nails for cutting. Face in, coat cut surface with it and let stand for ?(1/2 hr.), wipe off Nair, chill block and cut as routine. > > > From: caspake@yahoo.com > > Date: Tue, 17 Sep 2013 06:58:33 -0700 > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] Nail specimens > > > > Does anyone have a procedure for cutting nails and getting them to stay on the slide? Help please! > > > > Thanks, > > Cindy > > > > Sent from my iPhone > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Klaus.Kruttwig <@t> ucsf.edu Tue Sep 17 17:52:12 2013 From: Klaus.Kruttwig <@t> ucsf.edu (Kruttwig, Klaus) Date: Tue Sep 17 17:56:56 2013 Subject: [Histonet] longterm storage of tissue samples in 70% Ethanol Message-ID: Dear all, I have a question about longterm storage of histology samples and was wondering if someone can help me. I am not sure if my current sample storage system is optimal in terms of antigen- and histology preservation. Do you think it is okay to store 4% paraformaldehyde fixated samples before paraffin embedding for approx 2-3 years in 70% EtOH at 4 degree celsius? Or do I risk a decrease in specimen quality? Any help will be highly appreciated. Thanks, Klaus Klaus Kruttwig Department of Cell and Tissue Biology University of California - San Francisco San Franscisco, CA, USA From ibernard <@t> uab.edu Tue Sep 17 19:16:00 2013 From: ibernard <@t> uab.edu (Ian R Bernard) Date: Tue Sep 17 19:16:05 2013 Subject: [Histonet] Gastro Tissue for Validation of Processing Protocol Message-ID: Our lab is looking to acquire actual (gastric) or alternate similar tissue to validate a gastro tissue processing protocol. Any suggestions on obtaining endoscopic biopsy specimens or alternate tissue types to validate the gastric biopsy protocol? Ian R. Bernard Ian R. Bernard, MSHA, HT (ASCP) NCOIC-Manager, Anatomic Pathology Lab 10th Medical Group USAF Academy, CO 80840 From ggracie <@t> stvincents.com.au Tue Sep 17 19:31:06 2013 From: ggracie <@t> stvincents.com.au (Gary Gracie) Date: Tue Sep 17 19:31:15 2013 Subject: [Histonet] MYC antibody Message-ID: <4f2bf777310843f78fd4315523a6fffb@SVMHSEXCH02.svmhs.stvincents.com.au> Dear People from Histonet I need to know if somebody is using Anti Myc Protein Antibody in the Panel for Large Cell Lymphomas. I will appreciate any advice on the brand of the antibody and the protocol to make it work. I do not use any Automated Stainer, only Manual Immunohistochemistry. Thank You All in advance. Sincerely yours Cesar Romero Buenos Aires Argentina Dear Cesar We are also using the Myc from Epitomics for routine diagnostic lymphoma panels on humans. Dilution is 1/50. We are running Ventana Benchmark Ultras. Happy to suggest a protocol if you are using same. Regards Gary Gracie Senior Technical Officer IHC Laboratory Anatomical Pathology St Vincents Hospital Sydney, Australia ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the system manager. This footnote also confirms that this email message has been virus scanned and although no viruses were detected by the system, St Vincents & Mater Health Sydney accepts no liability for any consequential damage resulting from email containing any computer viruses. ********************************************************************** From Richard.Cartun <@t> hhchealth.org Tue Sep 17 19:40:47 2013 From: Richard.Cartun <@t> hhchealth.org (Cartun, Richard) Date: Tue Sep 17 19:40:53 2013 Subject: [Histonet] RE: MYC antibody In-Reply-To: <4f2bf777310843f78fd4315523a6fffb@SVMHSEXCH02.svmhs.stvincents.com.au> References: <4f2bf777310843f78fd4315523a6fffb@SVMHSEXCH02.svmhs.stvincents.com.au> Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E01884389@HHCEXCHMB05.hhcsystem.org> Epitomics has a good rabbit mAb. We do not do this routinely. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax richard.cartun@hhchealth.org ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Gary Gracie [ggracie@stvincents.com.au] Sent: Tuesday, September 17, 2013 8:31 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] MYC antibody Dear People from Histonet I need to know if somebody is using Anti Myc Protein Antibody in the Panel for Large Cell Lymphomas. I will appreciate any advice on the brand of the antibody and the protocol to make it work. I do not use any Automated Stainer, only Manual Immunohistochemistry. Thank You All in advance. Sincerely yours Cesar Romero Buenos Aires Argentina Dear Cesar We are also using the Myc from Epitomics for routine diagnostic lymphoma panels on humans. Dilution is 1/50. We are running Ventana Benchmark Ultras. Happy to suggest a protocol if you are using same. Regards Gary Gracie Senior Technical Officer IHC Laboratory Anatomical Pathology St Vincents Hospital Sydney, Australia ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the system manager. This footnote also confirms that this email message has been virus scanned and although no viruses were detected by the system, St Vincents & Mater Health Sydney accepts no liability for any consequential damage resulting from email containing any computer viruses. ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From leila.etemadi <@t> med.lu.se Wed Sep 18 02:58:09 2013 From: leila.etemadi <@t> med.lu.se (Leila Etemadi) Date: Wed Sep 18 02:58:36 2013 Subject: [Histonet] melanosome Message-ID: Hi every body, Is any one work on detecting melanosome with IHC technique ( preferably in the nervous system)?, I will be thankful to know your recommended antibody special animal laboratory. Cheers, Leila :-) From ree3 <@t> leicester.ac.uk Wed Sep 18 07:29:00 2013 From: ree3 <@t> leicester.ac.uk (Edwards, Richard E.) Date: Wed Sep 18 07:29:21 2013 Subject: [Histonet] complement immunohistochem. Message-ID: <7722595275A4DD4FA225B92CDBF174A101B0D6D980AF@EXC-MBX3.cfs.le.ac.uk> Hi All, I have been asked to immunostain for complement factors C3 and C1q, any help/opinions gratefully received, apparently cryostat is the preferred option, but most of our biopsies to date are in acetone-fixed GMA processed or formalin-fixed paraffin processed, many thanks in advance. Richard Edwards Leicester University U.K. From Dolores_Fischer <@t> baxter.com Wed Sep 18 08:27:26 2013 From: Dolores_Fischer <@t> baxter.com (Fischer, Dolores) Date: Wed Sep 18 08:27:32 2013 Subject: [Histonet] ZINC formalin vs NBF for IHC Message-ID: <7CA324454CDDCA40A56FC3C51923C0E4167E0D5A@026-SN2MPN1-031.026d.mgd.msft.net> Good morning everyone! I would like opinions on the use of zinc formalin for IHC. Pros and cons?? A new pathologist would like us to use zinc formalin for working out a protocol for CD34 and/or CD31 in mouse. I have always worked up needed protocols in 10% NBF with no problems and feel using zinc formalin will only throw in a variable that we don't need to add. I also feel that NBF is the fixative of choice for most antibodies and procedures. (small survey what do you use?) Am I not being open minded enough? What zinc formalin is recommended for use in a VIP processor and for IHC? I have read that there may be precipitation problems with this fixative. Opinions are appreciated, I did try to search the archives but didn't find too much information on this subject. I am trying to build a case for NOT using zinc formalin. I believe our pathologist is trying to save us the antigen retrieval step in IHC. Opinions?? Thanks all, Dolores The information transmitted is intended only for the person(s) or entity to which it is addressed and may contain confidential and/or legally privileged material. Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive privilege or confidentiality. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by entities other than the intended recipient is prohibited. If you receive this in error, please contact the sender and delete the material from any computer. For Translation: http://www.baxter.com/email_disclaimer From rjbuesa <@t> yahoo.com Wed Sep 18 09:21:25 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Sep 18 09:24:04 2013 Subject: [Histonet] longterm storage of tissue samples in 70% Ethanol In-Reply-To: References: Message-ID: <1379514085.84569.YahooMailNeo@web163103.mail.bf1.yahoo.com> Why do?you have to store fixed specimens for that long? If you are going to process them anyway, why don't you just process them and store as paraffin blocks? It will be much safer and you can be sure of the antigens preservation. Ren? J. ________________________________ From: "Kruttwig, Klaus" To: "histonet@lists.utsouthwestern.edu" Sent: Tuesday, September 17, 2013 6:52 PM Subject: [Histonet] longterm storage of tissue samples in 70% Ethanol Dear all, I have a question about longterm storage of histology samples and was wondering if someone can help me. I am not sure if my current sample storage system is optimal in terms of antigen- and histology preservation. Do you think it is okay to store 4% paraformaldehyde fixated samples before paraffin embedding for approx 2-3 years in 70% EtOH at 4 degree celsius? Or do I risk a decrease in specimen quality? Any help will be highly appreciated. Thanks, Klaus Klaus Kruttwig Department of Cell and Tissue Biology University of California - San Francisco San Franscisco, CA, USA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From contact <@t> excaliburpathology.com Wed Sep 18 09:24:58 2013 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Wed Sep 18 09:25:04 2013 Subject: [Histonet] ZINC formalin vs NBF for IHC In-Reply-To: <7CA324454CDDCA40A56FC3C51923C0E4167E0D5A@026-SN2MPN1-031.026d.mgd.msft.net> References: <7CA324454CDDCA40A56FC3C51923C0E4167E0D5A@026-SN2MPN1-031.026d.mgd.msft.net> Message-ID: <1379514298.32129.YahooMailNeo@web5702.biz.mail.ne1.yahoo.com> CD34 and CD31 do work better with a zinc formalin fixation, but you still need AR. The zinc will cause a precipitate from the phosphates. A few specimens will not create enough to be a problem. If a large number are needed, rinse the tissues first in PBS or 70%EtOH or process on the last run before changing the processor. ? Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 8901 S. Santa Fe, Suite G Oklahoma City, OK 73139 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ________________________________ From: "Fischer, Dolores" To: "histonet@lists.utsouthwestern.edu" Sent: Wednesday, September 18, 2013 8:27 AM Subject: [Histonet] ZINC formalin vs NBF for IHC Good morning everyone! I would like opinions on the use of zinc formalin for IHC.? Pros and cons??? A new pathologist would like us to use zinc formalin for working out a protocol for CD34 and/or CD31 in mouse.? I have always worked up needed protocols in 10% NBF with no problems and feel using zinc formalin will only throw in a variable that we don't need to add.? I also feel that NBF is the fixative of choice for most antibodies and procedures.? (small survey what do you use?)? Am I not being open minded enough? What zinc formalin is recommended for use in a VIP processor and for IHC?? I have read that there may be precipitation problems with this fixative.? Opinions are appreciated, I did try to search the archives but didn't find too much information on this subject.? I am trying to build a case for NOT using zinc formalin.? I believe our pathologist is trying to save us the antigen retrieval step in IHC.? Opinions?? Thanks all, Dolores The information transmitted is intended only for the person(s) or entity to which it is addressed and may contain confidential and/or legally privileged material. Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive privilege or confidentiality. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by entities other than the intended recipient is prohibited. If you receive this in error, please contact the sender and delete the material from any computer. For Translation: http://www.baxter.com/email_disclaimer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpwenk <@t> sbcglobal.net Wed Sep 18 09:30:12 2013 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Wed Sep 18 09:30:25 2013 Subject: [Histonet] Handouts - NSH Symposium Message-ID: <2E25C3323E2A4F61A1B2C619275A63AB@HP2010> Giving everyone going to the NSH Symposium a heads up, in case they missed reading the NSH email about handouts. NSH will NOT be providing printed out handouts for the workshops. They are going green. NSH sent out an email around Sept. 10 (that?s when I received the email), that had a subject line of ?download your handouts?. If you want handouts for the workshops you signed up for, the email directed you to a link, in which you put in your email address and a password they provided you. You then go to your workshops/lectures that you signed up for, and print off the PDF?s of the handouts. I?ve talked with a couple of people recently, who didn?t realize this, so I thought getting this information out another way through Histonet might be helpful to those attending. Peggy A. Wenk, HTL(ASCP)SLS From cmiller <@t> gladstone.ucsf.edu Wed Sep 18 09:43:54 2013 From: cmiller <@t> gladstone.ucsf.edu (Caroline Miller) Date: Wed Sep 18 09:44:03 2013 Subject: [Histonet] longterm storage of tissue samples in 70% Ethanol In-Reply-To: <1379514085.84569.YahooMailNeo@web163103.mail.bf1.yahoo.com> References: <1379514085.84569.YahooMailNeo@web163103.mail.bf1.yahoo.com> Message-ID: <5906ED10-0B9E-4EA4-BA86-A60C949CA9D8@gladstone.ucsf.edu> Sometimes in the research field or is necessary to store up samples without processing, either waiting for other samples from the set, or the cost to the researcher of processing tissue that they might not need We always advise our users to fix adequately and then store in 70% until they process. We have never had a problem with this, even when samples have been stored like this for months The theory is that there is just enough alcohol to prevent dehydration or rehydration and it is in 'steady state' Any concrete evidence to this fact would be greatly welcomed though, I am going on completely empirical evidence! Yours Caroline Caroline Miller Gladstone Institutes www.gladstoneinstitutes.org Tel: 415 7342566 Cell: 415 2187297 On Sep 18, 2013, at 7:21 AM, Rene J Buesa wrote: > Why do you have to store fixed specimens for that long? If you are going to process them anyway, why don't you just process them and store as paraffin blocks? It will be much safer and you can be sure of the antigens preservation. > Ren? J. > > > ________________________________ > From: "Kruttwig, Klaus" > To: "histonet@lists.utsouthwestern.edu" > Sent: Tuesday, September 17, 2013 6:52 PM > Subject: [Histonet] longterm storage of tissue samples in 70% Ethanol > > > Dear all, > > I have a question about longterm storage of histology samples and was wondering if someone can help me. > I am not sure if my current sample storage system is optimal in terms of antigen- and histology preservation. Do you think it is okay to store 4% paraformaldehyde fixated samples before paraffin embedding for approx 2-3 years in 70% EtOH at 4 degree celsius? Or do I risk a decrease in specimen quality? > > Any help will be highly appreciated. > > Thanks, > Klaus > > Klaus Kruttwig > Department of Cell and Tissue Biology > University of California - San Francisco > San Franscisco, CA, USA > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Nagy_Natalie <@t> holyokehealth.com Wed Sep 18 09:43:44 2013 From: Nagy_Natalie <@t> holyokehealth.com (Natalie Nagy) Date: Wed Sep 18 09:44:07 2013 Subject: [Histonet] Bringing new antibodies in house - Ki-67,P40,PAX8 Message-ID: <523983E00200000F0033C7E8@hh11.holyokehealth.com> Hello Everyone, I am currently looking to bring a few new antibodies in house; KI-67,P40, and PAX8. I have not worked with these antibodies yet and wanted to get some opinions from everyone. For P40 and PAX8; are you using a monoclonal or polyclonal? Also what company are you getting them from? My hospital deals mainly with Dako, Biocare, and Cellmarque. We are a very small facility that does HIER with a waterbath, and then manual IHC pipetting with the old sequenza coverplate tiles. So usually we prefer concentrate for antibodies not pre-dilute because of the longer expiration times, and of course the cost. So I guess what I am asking is, what clone do you prefer. What company do you get it from? How much does it cost? And have you ever run it on a manual setup, if so how was it to work with? Thanks for all the help in advance, Natalie J. Nagy Histology Supervisor Holyoke Medical Center nagy_natalie@holyokehealth.com CONFIDENTIALITY NOTICE: This email communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please reply to the sender immediately and destroy all copies of this communication and any attachments. For further information regarding Holyoke Medical Center's privacy policy, Please visit our Internet web site at http://www.holyokehealth.com From rjbuesa <@t> yahoo.com Wed Sep 18 10:12:29 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Sep 18 10:12:34 2013 Subject: [Histonet] longterm storage of tissue samples in 70% Ethanol In-Reply-To: <5906ED10-0B9E-4EA4-BA86-A60C949CA9D8@gladstone.ucsf.edu> References: <1379514085.84569.YahooMailNeo@web163103.mail.bf1.yahoo.com> <5906ED10-0B9E-4EA4-BA86-A60C949CA9D8@gladstone.ucsf.edu> Message-ID: <1379517149.77683.YahooMailNeo@web163102.mail.bf1.yahoo.com> Caroline: I see your point and it is not infrequent that tissues are stored in 70EthOL for long periods but, as far as I know, there are no studies on the subject. Since you are in the field of research why don't you do an experiment? You could take different types of tissues, the most used perhaps, an fix them?under optimal conditions. Then immediately process 1 piece of each and store it included in paraffin. The rest store them in 70EthOL and process one piece monthly during the first year; bimonthly during the second and quarterly during the third year. At the end make sections of each one and evaluate the epitope signal and characteristics. You may come up with a very interesting paper to publish and solve this long lasting problem. Just an idea. Ren? J. ________________________________ From: Caroline Miller To: Rene J Buesa Cc: "Kruttwig, Klaus" ; "histonet@lists.utsouthwestern.edu" Sent: Wednesday, September 18, 2013 10:43 AM Subject: Re: [Histonet] longterm storage of tissue samples in 70% Ethanol Sometimes in the research field or is necessary to store up samples without processing, either waiting for other samples from the set, or the cost to the researcher of processing tissue that they might not need We always advise our users to fix adequately and then store in 70% until they process. We have never had a problem with this, even when samples have been stored like this for months The theory is that there is just enough alcohol to prevent dehydration or rehydration and it is in 'steady state' Any concrete evidence to this fact would be greatly welcomed though, I am going on completely empirical evidence! Yours Caroline Caroline Miller Gladstone Institutes www.gladstoneinstitutes.org Tel: 415 7342566 Cell: 415 2187297 On Sep 18, 2013, at 7:21 AM, Rene J Buesa wrote: > Why do you have to store fixed specimens for that long? If you are going to process them anyway, why don't you just process them and store as paraffin blocks? It will be much safer and you can be sure of the antigens preservation. > Ren? J. > > > ________________________________ > From: "Kruttwig, Klaus" > To: "histonet@lists.utsouthwestern.edu" > Sent: Tuesday, September 17, 2013 6:52 PM > Subject: [Histonet] longterm storage of tissue samples in 70% Ethanol > > > Dear all, > > I have a question about longterm storage of histology samples and was wondering if someone can help me. > I am not sure if my current sample storage system is optimal in terms of antigen- and histology preservation. Do you think it is okay to store 4% paraformaldehyde fixated samples before paraffin embedding for approx 2-3 years in 70% EtOH at 4 degree celsius? Or do I risk a decrease in specimen quality? > > Any help will be highly appreciated. > > Thanks, > Klaus > > Klaus Kruttwig > Department of Cell and Tissue Biology > University of California - San Francisco > San Franscisco, CA, USA > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Klaus.Kruttwig <@t> ucsf.edu Wed Sep 18 11:23:55 2013 From: Klaus.Kruttwig <@t> ucsf.edu (Kruttwig, Klaus) Date: Wed Sep 18 11:28:47 2013 Subject: [Histonet] longterm storage of tissue samples in 70% Ethanol In-Reply-To: <1379517149.77683.YahooMailNeo@web163102.mail.bf1.yahoo.com> References: <1379514085.84569.YahooMailNeo@web163103.mail.bf1.yahoo.com> <5906ED10-0B9E-4EA4-BA86-A60C949CA9D8@gladstone.ucsf.edu>, <1379517149.77683.YahooMailNeo@web163102.mail.bf1.yahoo.com> Message-ID: Dear All, thank you very much for your replies. It's exactly the point that embedding is quite expensive and my tissue collection is growing. Also another reason is that I have seen a couple of papers stating that antigenicity loss in formalin-fixed paraffin-embedded tissue is also not guaranteed and some protein degradation can occur. As a result I thought storing in 70% EtOH might be better... I was really thinking about publishing a paper on it, as Rene suggested, maybe including two methods of antigen retrieval conditions. ________________________________ From: Rene J Buesa [rjbuesa@yahoo.com] Sent: Wednesday, September 18, 2013 8:12 AM To: Miller, Caroline Cc: Kruttwig, Klaus; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] longterm storage of tissue samples in 70% Ethanol Caroline: I see your point and it is not infrequent that tissues are stored in 70EthOL for long periods but, as far as I know, there are no studies on the subject. Since you are in the field of research why don't you do an experiment? You could take different types of tissues, the most used perhaps, an fix them under optimal conditions. Then immediately process 1 piece of each and store it included in paraffin. The rest store them in 70EthOL and process one piece monthly during the first year; bimonthly during the second and quarterly during the third year. At the end make sections of each one and evaluate the epitope signal and characteristics. You may come up with a very interesting paper to publish and solve this long lasting problem. Just an idea. Ren? J. From: Caroline Miller To: Rene J Buesa Cc: "Kruttwig, Klaus" ; "histonet@lists.utsouthwestern.edu" Sent: Wednesday, September 18, 2013 10:43 AM Subject: Re: [Histonet] longterm storage of tissue samples in 70% Ethanol Sometimes in the research field or is necessary to store up samples without processing, either waiting for other samples from the set, or the cost to the researcher of processing tissue that they might not need We always advise our users to fix adequately and then store in 70% until they process. We have never had a problem with this, even when samples have been stored like this for months The theory is that there is just enough alcohol to prevent dehydration or rehydration and it is in 'steady state' Any concrete evidence to this fact would be greatly welcomed though, I am going on completely empirical evidence! Yours Caroline Caroline Miller Gladstone Institutes www.gladstoneinstitutes.org Tel: 415 7342566 Cell: 415 2187297 On Sep 18, 2013, at 7:21 AM, Rene J Buesa > wrote: > Why do you have to store fixed specimens for that long? If you are going to process them anyway, why don't you just process them and store as paraffin blocks? It will be much safer and you can be sure of the antigens preservation. > Ren? J. > > > ________________________________ > From: "Kruttwig, Klaus" > > To: "histonet@lists.utsouthwestern.edu" > > Sent: Tuesday, September 17, 2013 6:52 PM > Subject: [Histonet] longterm storage of tissue samples in 70% Ethanol > > > Dear all, > > I have a question about longterm storage of histology samples and was wondering if someone can help me. > I am not sure if my current sample storage system is optimal in terms of antigen- and histology preservation. Do you think it is okay to store 4% paraformaldehyde fixated samples before paraffin embedding for approx 2-3 years in 70% EtOH at 4 degree celsius? Or do I risk a decrease in specimen quality? > > Any help will be highly appreciated. > > Thanks, > Klaus > > Klaus Kruttwig > Department of Cell and Tissue Biology > University of California - San Francisco > San Franscisco, CA, USA > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TJohnson <@t> gnf.org Wed Sep 18 12:00:28 2013 From: TJohnson <@t> gnf.org (Teri Johnson) Date: Wed Sep 18 12:00:42 2013 Subject: [Histonet] Re: ZINC formalin vs NBF for IHC Message-ID: <9F3CFEE76E51B64991C7485270890B40497C6238@EX5.lj.gnf.org> Hi Dolores, First I need to correct something answered previously. Paula Pierce is absolute correct that zinc will precipitate in the presence of phosphates. You do not want to move them out of Zinc Formalin into regular buffered formalin. However she did say to rinse tissues in PBS or 70% EtOH, but PBS is a phosphate buffer so do not use that. Use regular water, DI or tap, instead. Then place it in alcohol or even regular buffered formalin. Do not put Zinc Formalin on your processor. Ideally you would want to fix in Zinc Formalin and then start processing in alcohol on your processor. Most places have formalin as their first two stations on the processor so it is difficult to change that in the bigger scheme. When we used this Zinc Formalin in the clinical lab, we would fix for 6-7 hours in Zinc formalin, rinse in water, and then put on the processor with the rest of the tissues and it worked ok. It does make a difference in antigenicity with some antibodies. But we were never able to completely get rid of antigen retrieval. We just got better staining. You would need to go to a non-formalin type fixative (glycoxal) or use Beckstead's Zinc fixative. That's not something you would want to use routinely but only in special situations. Morphology doesn't look the same and that can cause some issues for some pathologists. There is a good CD31 antibody out there that works in NBF fixed mouse tissue. Dianova makes a rat anti-mouse, DIA-310 is the catalog number. I don't have one listed for CD34, but I bet someone out there has one. As for your processes, only you and your pathologist can determine what works best for you. We had no issue using Zinc Formalin in a clinical lab for lymphoma workup and bone marrow specimens. Do not over-fix, it penetrates and hardens tissues more quickly than NBF. Rinse in water and then process using 70% alcohol as your first step if you can. It is possible to successfully integrate this into your lab even if you have to go into NBF on the processor. You will need Zinc formalin fixed control tissues processed the same and will need to re-validate your antibody staining using those. That usually only requires a titration run, which is what you would do with a new antibody lot anyway. Teri Johnson Manager, Histology Genomics Institute for Novartis Research Foundation San Diego, CA 858-332-4752 From cls71877 <@t> gmail.com Wed Sep 18 12:02:31 2013 From: cls71877 <@t> gmail.com (Cristi Rigazio) Date: Wed Sep 18 12:02:43 2013 Subject: [Histonet] Handouts - NSH Symposium In-Reply-To: <2E25C3323E2A4F61A1B2C619275A63AB@HP2010> References: <2E25C3323E2A4F61A1B2C619275A63AB@HP2010> Message-ID: Thank you Peggy! Sent from my iPhone On Sep 18, 2013, at 7:30 AM, "Lee & Peggy Wenk" wrote: > Giving everyone going to the NSH Symposium a heads up, in case they missed reading the NSH email about handouts. > > NSH will NOT be providing printed out handouts for the workshops. They are going green. > > NSH sent out an email around Sept. 10 (that?s when I received the email), that had a subject line of ?download your handouts?. If you want handouts for the workshops you signed up for, the email directed you to a link, in which you put in your email address and a password they provided you. You then go to your workshops/lectures that you signed up for, and print off the PDF?s of the handouts. > > I?ve talked with a couple of people recently, who didn?t realize this, so I thought getting this information out another way through Histonet might be helpful to those attending. > > Peggy A. Wenk, HTL(ASCP)SLS > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Wed Sep 18 12:22:00 2013 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Wed Sep 18 12:21:57 2013 Subject: [Histonet] RE: Re: ZINC formalin vs NBF for IHC In-Reply-To: <9F3CFEE76E51B64991C7485270890B40497C6238@EX5.lj.gnf.org> References: <9F3CFEE76E51B64991C7485270890B40497C6238@EX5.lj.gnf.org> Message-ID: <14E2C6176416974295479C64A11CB9AE019BB56FE15E@SBS2K8.premierlab.local> If you are looking for a good antibody to CD34 that works in mouse - here is one. CD34 abcam ab8158 MEC 14.7 Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Laboratory Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 Work (303) 682-3949 Fax (303) 682-9060 Cell (303) 881-0763 liz@premierlab.com www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Teri Johnson Sent: Wednesday, September 18, 2013 11:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: ZINC formalin vs NBF for IHC Hi Dolores, First I need to correct something answered previously. Paula Pierce is absolute correct that zinc will precipitate in the presence of phosphates. You do not want to move them out of Zinc Formalin into regular buffered formalin. However she did say to rinse tissues in PBS or 70% EtOH, but PBS is a phosphate buffer so do not use that. Use regular water, DI or tap, instead. Then place it in alcohol or even regular buffered formalin. Do not put Zinc Formalin on your processor. Ideally you would want to fix in Zinc Formalin and then start processing in alcohol on your processor. Most places have formalin as their first two stations on the processor so it is difficult to change that in the bigger scheme. When we used this Zinc Formalin in the clinical lab, we would fix for 6-7 hours in Zinc formalin, rinse in water, and then put on the processor with the rest of the tissues and it worked ok. It does make a difference in antigenicity with some antibodies. But we were never able to completely get rid of antigen retrieval. We just got better staining. You would need to go to a non-formalin type fixative (glycoxal) or use Beckstead's Zinc fixative. That's not something you would want to use routinely but only in special situations. Morphology doesn't look the same and that can cause some issues for some pathologists. There is a good CD31 antibody out there that works in NBF fixed mouse tissue. Dianova makes a rat anti-mouse, DIA-310 is the catalog number. I don't have one listed for CD34, but I bet someone out there has one. As for your processes, only you and your pathologist can determine what works best for you. We had no issue using Zinc Formalin in a clinical lab for lymphoma workup and bone marrow specimens. Do not over-fix, it penetrates and hardens tissues more quickly than NBF. Rinse in water and then process using 70% alcohol as your first step if you can. It is possible to successfully integrate this into your lab even if you have to go into NBF on the processor. You will need Zinc formalin fixed control tissues processed the same and will need to re-validate your antibody staining using those. That usually only requires a titration run, which is what you would do with a new antibody lot anyway. Teri Johnson Manager, Histology Genomics Institute for Novartis Research Foundation San Diego, CA 858-332-4752 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From barbara.tibbs <@t> accuratediagnosticlabs.com Wed Sep 18 12:48:03 2013 From: barbara.tibbs <@t> accuratediagnosticlabs.com (Barbara Tibbs) Date: Wed Sep 18 12:48:30 2013 Subject: [Histonet] RE: Gastro Tissue for Validation of Processing Protocol In-Reply-To: References: Message-ID: What I did when validating a processor for small biopsies was to cut tiny, biopsy-sized sections of tissue from larger specimens such as stomach, colon, skin, etc. We inquired at a local hospital to see if we could get specimens and they graciously donated tissues that otherwise would have been discarded. The validation process went well and I use the protocol to process patient biopsies at present. If you have access to these types of tissue, particularly stomach since that is what is your interest, you might try this technique. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.tibbs@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Ian R Bernard Sent: Tuesday, September 17, 2013 11:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Gastro Tissue for Validation of Processing Protocol Our lab is looking to acquire actual (gastric) or alternate similar tissue to validate a gastro tissue processing protocol. Any suggestions on obtaining endoscopic biopsy specimens or alternate tissue types to validate the gastric biopsy protocol? Ian R. Bernard Ian R. Bernard, MSHA, HT (ASCP) NCOIC-Manager, Anatomic Pathology Lab 10th Medical Group USAF Academy, CO 80840 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Tracey.Lenek <@t> cls.ab.ca Wed Sep 18 12:51:48 2013 From: Tracey.Lenek <@t> cls.ab.ca (Tracey Lenek) Date: Wed Sep 18 12:51:54 2013 Subject: [Histonet] Cerner Millennium Message-ID: <2DFAEEFF192A9141ABACFF88BE613BF3B5506547D8@EXMBXC1.crha.bewell.ca> Hi, We are a large multi-site facility and are in the midst of investigating the potential for Pathologists to order ancillary/additional testing in PTOE within our Millennium LIS. As we have blocks at multiple locations and a large number of Pathologists, the list of ordering requests becomes quite large and difficult for our technical stream to manage. Does anyone have any advice as to how to better manage the on line ordering process using Cerner Millennium? Many thanks, Tracey Lenek Anatomic Pathology/Cytology Manager Calgary Lab Services 403-770-3448 ________________________________ This message and any attached documents are only for the use of the intended recipient(s), are confidential and may contain privileged information. Any unauthorized review, use, retransmission, or other disclosure is strictly prohibited. If you have received this message in error, please notify the sender immediately, and then delete the original message. Thank you. From algranth <@t> email.arizona.edu Wed Sep 18 13:06:36 2013 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Wed Sep 18 13:07:38 2013 Subject: [Histonet] section thickness for Picro sirius red stain Message-ID: I've been doing this PSR stain for a few years now on various types of tissue sections of pretty much routine thickness (4-5 microns). I was asked today if the stain would penetrate a thicker section - like 40 or more microns. Even a vibratome section. The tissue is pig optic nerve and a small bit of surrounding tissue. When stained with PSR, the routine thickness sections were awesome. Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 From cforster <@t> umn.edu Wed Sep 18 13:42:15 2013 From: cforster <@t> umn.edu (Colleen Forster) Date: Wed Sep 18 13:42:25 2013 Subject: [Histonet] unsubscribe for NSH Message-ID: <5239F407.9050709@umn.edu> From chapcl <@t> yahoo.com Wed Sep 18 13:44:23 2013 From: chapcl <@t> yahoo.com (Will Chappell) Date: Wed Sep 18 13:44:32 2013 Subject: [Histonet] unsubscribe for NSH In-Reply-To: <5239F407.9050709@umn.edu> References: <5239F407.9050709@umn.edu> Message-ID: <75305FD8-9880-434E-957D-6976FC03435D@yahoo.com> Oh dear lord. Not Colleen!! Why man hath wrought?? Sent from my iPhone On Sep 18, 2013, at 11:42 AM, Colleen Forster wrote: > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Wed Sep 18 13:49:23 2013 From: jqb7 <@t> cdc.gov (Sanders, Jeanine (CDC/OID/NCEZID)) Date: Wed Sep 18 13:50:11 2013 Subject: [Histonet] unsubscribe for NSH In-Reply-To: <75305FD8-9880-434E-957D-6976FC03435D@yahoo.com> References: <5239F407.9050709@umn.edu> <75305FD8-9880-434E-957D-6976FC03435D@yahoo.com> Message-ID: I am laughing so hard right now................surely she is pranking us? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Will Chappell Sent: Wednesday, September 18, 2013 2:44 PM Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] unsubscribe for NSH Oh dear lord. Not Colleen!! Why man hath wrought?? Sent from my iPhone On Sep 18, 2013, at 11:42 AM, Colleen Forster wrote: > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From trathborne <@t> somerset-healthcare.com Wed Sep 18 13:54:47 2013 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Wed Sep 18 13:55:31 2013 Subject: [Histonet] RE: Cerner Millennium In-Reply-To: <2DFAEEFF192A9141ABACFF88BE613BF3B5506547D8@EXMBXC1.crha.bewell.ca> References: <2DFAEEFF192A9141ABACFF88BE613BF3B5506547D8@EXMBXC1.crha.bewell.ca> Message-ID: <3AD061FE740D464FAC7BF6B5CFB75707A8A6A326@smcmail02.somerset-healthcare.com> Have you tried asking on UCern? uCern Admin or https://cernercare.com/accounts/login?returnTo=https%3A%2F%2Fconnect.ucern.com%2Fjiverp%2Fopenidendpoint.jspa%3Fprovider%3Dhttps%253A%252F%252Fcernercare.com%252Faccounts%252Fopenid%26destination%3D%252Femaildigestprofile.jspa# uCern is a forum similar to histonet. You should be able to click on the link above and select preferences. I receive weekly updates, and have found some very good suggestions on there. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tracey Lenek Sent: Wednesday, September 18, 2013 1:52 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Cerner Millennium Hi, We are a large multi-site facility and are in the midst of investigating the potential for Pathologists to order ancillary/additional testing in PTOE within our Millennium LIS. As we have blocks at multiple locations and a large number of Pathologists, the list of ordering requests becomes quite large and difficult for our technical stream to manage. Does anyone have any advice as to how to better manage the on line ordering process using Cerner Millennium? Many thanks, Tracey Lenek Anatomic Pathology/Cytology Manager Calgary Lab Services 403-770-3448 ________________________________ This message and any attached documents are only for the use of the intended recipient(s), are confidential and may contain privileged information. Any unauthorized review, use, retransmission, or other disclosure is strictly prohibited. If you have received this message in error, please notify the sender immediately, and then delete the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brett_connolly <@t> merck.com Wed Sep 18 13:56:34 2013 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Wed Sep 18 13:56:45 2013 Subject: [Histonet] unsubscribe for NSH In-Reply-To: <75305FD8-9880-434E-957D-6976FC03435D@yahoo.com> References: <5239F407.9050709@umn.edu> <75305FD8-9880-434E-957D-6976FC03435D@yahoo.com> Message-ID: Hey - give her SOME credit...she didn't 'uscribe' , 'unscribe' or 'unsuscribe' -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Will Chappell Sent: Wednesday, September 18, 2013 2:44 PM Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] unsubscribe for NSH Oh dear lord. Not Colleen!! Why man hath wrought?? Sent from my iPhone On Sep 18, 2013, at 11:42 AM, Colleen Forster wrote: > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From turkekul <@t> gmail.com Wed Sep 18 17:29:09 2013 From: turkekul <@t> gmail.com (Mesru T) Date: Wed Sep 18 17:29:14 2013 Subject: [Histonet] ZInc formalin Message-ID: Dear Histonetters, I do not think there is a good CD31 (PECAM-1) antibody that will work on FFPE mouse tissues. If you have one please let me know. We use CD34 rat mono (Abcam) and Meca-32 Rat mono (Dev Hybridoma Bank Iowa Univ.) to label endothelial cells of blood vessels in FFPE mouse tissues. There is a CD31 Rat mono from BD that will work of 10% NBF fixed, sucrose protected cryo embedded mouse tissues with antigen retrieval. The same CD31 Rat mono from BD will also work on Zinc Fixative from BD cat# 51-7538KZ ( it is a solution of ZnCl2 and maybe other zinc salts but no aldehyde) fixed paraffin embedded mouse tissues. The morphology of the section will be suboptimal due to this novel fixative. If anybody has any experince with this Zinc Fixative please would you share? Regards, Mesruh Turkekul mskcc.org From liz <@t> premierlab.com Wed Sep 18 18:51:38 2013 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Wed Sep 18 18:52:30 2013 Subject: [Histonet] ZInc formalin In-Reply-To: References: Message-ID: <14E2C6176416974295479C64A11CB9AE019BB5702266@SBS2K8.premierlab.local> Dianova has an excellent antibody for CD31 in mice for FFPE tissue samples. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 881-0763 cell (303) 682-9060 fax liz@premierlab.com Ship to address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mesru T [turkekul@gmail.com] Sent: Wednesday, September 18, 2013 4:29 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ZInc formalin Dear Histonetters, I do not think there is a good CD31 (PECAM-1) antibody that will work on FFPE mouse tissues. If you have one please let me know. We use CD34 rat mono (Abcam) and Meca-32 Rat mono (Dev Hybridoma Bank Iowa Univ.) to label endothelial cells of blood vessels in FFPE mouse tissues. There is a CD31 Rat mono from BD that will work of 10% NBF fixed, sucrose protected cryo embedded mouse tissues with antigen retrieval. The same CD31 Rat mono from BD will also work on Zinc Fixative from BD cat# 51-7538KZ ( it is a solution of ZnCl2 and maybe other zinc salts but no aldehyde) fixed paraffin embedded mouse tissues. The morphology of the section will be suboptimal due to this novel fixative. If anybody has any experince with this Zinc Fixative please would you share? Regards, Mesruh Turkekul mskcc.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ggracie <@t> stvincents.com.au Wed Sep 18 19:20:51 2013 From: ggracie <@t> stvincents.com.au (Gary Gracie) Date: Wed Sep 18 19:21:01 2013 Subject: [Histonet] p40 antibody Message-ID: Dear Natalie, I can't help you with PAX8, and for Ki67 we use a Ventana pre-dilute. I can recommend Biocare Medical polyclonal p40, cat#ACI3030B. I use it diluted 1/100. Regards Gary Gracie Senior Technical Officer IHC Laboratory Anatomical Pathology St Vincents Hospital Sydney, Australia Hello Everyone, I am currently looking to bring a few new antibodies in house; KI-67,P40, and PAX8. I have not worked with these antibodies yet and wanted to get some opinions from everyone. For P40 and PAX8; are you using a monoclonal or polyclonal? Also what company are you getting them from? My hospital deals mainly with Dako, Biocare, and Cellmarque. We are a very small facility that does HIER with a waterbath, and then manual IHC pipetting with the old sequenza coverplate tiles. So usually we prefer concentrate for antibodies not pre-dilute because of the longer expiration times, and of course the cost. So I guess what I am asking is, what clone do you prefer. What company do you get it from? How much does it cost? And have you ever run it on a manual setup, if so how was it to work with? Thanks for all the help in advance, Natalie J. Nagy Histology Supervisor Holyoke Medical Center nagy_natalie@holyokehealth.com ************************** ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the system manager. This footnote also confirms that this email message has been virus scanned and although no viruses were detected by the system, St Vincents & Mater Health Sydney accepts no liability for any consequential damage resulting from email containing any computer viruses. ********************************************************************** From turkekul <@t> gmail.com Wed Sep 18 20:32:49 2013 From: turkekul <@t> gmail.com (Mesruh Turkekul) Date: Wed Sep 18 20:32:56 2013 Subject: [Histonet] ZInc formalin In-Reply-To: <14E2C6176416974295479C64A11CB9AE019BB5702266@SBS2K8.premierlab.local> References: <14E2C6176416974295479C64A11CB9AE019BB5702266@SBS2K8.premierlab.local> Message-ID: Hi Liz, I checked the web site and the images of FFPE sections stained with CD31 from Dianova. It seems that it mostly stains large blood vessels. Which I have experienced with most CD31 Ab on the market. Does this ab stain all the capillaries in your experiments? Thanks again, Mesruh Sent from my iPad On Sep 18, 2013, at 7:51 PM, Elizabeth Chlipala wrote: > Dianova has an excellent antibody for CD31 in mice for FFPE tissue samples. > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > Premier Laboratory, LLC > PO Box 18592 > Boulder, CO 80308 > (303) 682-3949 office > (303) 881-0763 cell > (303) 682-9060 fax > liz@premierlab.com > > Ship to address: > > Premier Laboratory, LLC > 1567 Skyway Drive, Unit E > Longmont, CO 80504 > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mesru T [turkekul@gmail.com] > Sent: Wednesday, September 18, 2013 4:29 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] ZInc formalin > > Dear Histonetters, > > I do not think there is a good CD31 (PECAM-1) antibody that will work on > FFPE mouse tissues. If you have one please let me know. We use CD34 rat > mono (Abcam) and Meca-32 Rat mono (Dev Hybridoma Bank Iowa Univ.) to label > endothelial cells of blood vessels in FFPE mouse tissues. > There is a CD31 Rat mono from BD that will work of 10% NBF fixed, sucrose > protected cryo embedded mouse tissues with antigen retrieval. > The same CD31 Rat mono from BD will also work on Zinc Fixative from BD > cat# 51-7538KZ ( it is a solution of ZnCl2 and maybe other zinc salts but > no aldehyde) fixed paraffin embedded mouse tissues. The morphology of the > section will be suboptimal due to this novel fixative. > If anybody has any experince with this Zinc Fixative please would you share? > > Regards, > > Mesruh Turkekul > mskcc.org > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From fbozkurt <@t> gmail.com Wed Sep 18 23:40:31 2013 From: fbozkurt <@t> gmail.com (Mehmet Fatih BOZKURT) Date: Wed Sep 18 23:40:40 2013 Subject: [Histonet] ZInc formalin In-Reply-To: References: <14E2C6176416974295479C64A11CB9AE019BB5702266@SBS2K8.premierlab.local> Message-ID: Santa Cruz, SC-1506 is a good goat polyclonal antibody for mouse tissues. also there is rabbit IgG that is coded SC-1506R. On Thu, Sep 19, 2013 at 4:32 AM, Mesruh Turkekul wrote: > Hi Liz, > > I checked the web site and the images of FFPE sections stained with CD31 > from Dianova. It seems that it mostly stains large blood vessels. Which I > have experienced with most CD31 Ab on the market. Does this ab stain all > the capillaries in your experiments? > > Thanks again, > Mesruh > > Sent from my iPad > > On Sep 18, 2013, at 7:51 PM, Elizabeth Chlipala > wrote: > > > Dianova has an excellent antibody for CD31 in mice for FFPE tissue > samples. > > > > Liz > > > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > > Premier Laboratory, LLC > > PO Box 18592 > > Boulder, CO 80308 > > (303) 682-3949 office > > (303) 881-0763 cell > > (303) 682-9060 fax > > liz@premierlab.com > > > > Ship to address: > > > > Premier Laboratory, LLC > > 1567 Skyway Drive, Unit E > > Longmont, CO 80504 > > ________________________________________ > > From: histonet-bounces@lists.utsouthwestern.edu [ > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mesru T [ > turkekul@gmail.com] > > Sent: Wednesday, September 18, 2013 4:29 PM > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] ZInc formalin > > > > Dear Histonetters, > > > > I do not think there is a good CD31 (PECAM-1) antibody that will work on > > FFPE mouse tissues. If you have one please let me know. We use CD34 rat > > mono (Abcam) and Meca-32 Rat mono (Dev Hybridoma Bank Iowa Univ.) to > label > > endothelial cells of blood vessels in FFPE mouse tissues. > > There is a CD31 Rat mono from BD that will work of 10% NBF fixed, sucrose > > protected cryo embedded mouse tissues with antigen retrieval. > > The same CD31 Rat mono from BD will also work on Zinc Fixative from BD > > cat# 51-7538KZ ( it is a solution of ZnCl2 and maybe other zinc salts but > > no aldehyde) fixed paraffin embedded mouse tissues. The morphology of the > > section will be suboptimal due to this novel fixative. > > If anybody has any experince with this Zinc Fixative please would you > share? > > > > Regards, > > > > Mesruh Turkekul > > mskcc.org > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Mehmet Fatih BOZKURT, DVM, PhD Afyon Kocatepe University Faculty of Veterinary Medicine Department of Pathology 03030, ANS Campus Afyonkarahisar-TURKEY Tel: +902722281312-16173/16237 From trathborne <@t> somerset-healthcare.com Thu Sep 19 08:55:11 2013 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Thu Sep 19 08:55:37 2013 Subject: [Histonet] Thermo Scientific HM 550 Cryostat Message-ID: <3AD061FE740D464FAC7BF6B5CFB75707A8A6A6F9@smcmail02.somerset-healthcare.com> Looking for opinions on this cryostat. Has anyone used it? What is the Cold D like to use? How often is the solution replaced? What about the Vacutome? It sounds like a nice feature along with the Automatic Cryo-Approach. Appreciate all responses. Thanks, Toni From liz <@t> premierlab.com Thu Sep 19 09:42:02 2013 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Thu Sep 19 09:42:02 2013 Subject: [Histonet] ZInc formalin In-Reply-To: References: <14E2C6176416974295479C64A11CB9AE019BB5702266@SBS2K8.premierlab.local> Message-ID: <14E2C6176416974295479C64A11CB9AE019BB56FE177@SBS2K8.premierlab.local> Hello All Unless something has changed, this is how I remember it. That antibody used to work for mouse on FFPE tissue samples ( I had a lot that did work and it was great, but that was years ago) and then the goat died or something like that??????. From what I understand now the current lot numbers do not work on FFPE tissues samples. Has something changed recently that this antibody will work again in FFPE mouse tissues, if so I'm interested. That antibody worked on multiple species quite well before. Thanks Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Laboratory Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 Work (303) 682-3949 Fax (303) 682-9060 Cell (303) 881-0763 liz@premierlab.com www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mehmet Fatih BOZKURT Sent: Wednesday, September 18, 2013 10:41 PM To: Mesruh Turkekul; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] ZInc formalin Santa Cruz, SC-1506 is a good goat polyclonal antibody for mouse tissues. also there is rabbit IgG that is coded SC-1506R. On Thu, Sep 19, 2013 at 4:32 AM, Mesruh Turkekul wrote: > Hi Liz, > > I checked the web site and the images of FFPE sections stained with > CD31 from Dianova. It seems that it mostly stains large blood vessels. > Which I have experienced with most CD31 Ab on the market. Does this ab > stain all the capillaries in your experiments? > > Thanks again, > Mesruh > > Sent from my iPad > > On Sep 18, 2013, at 7:51 PM, Elizabeth Chlipala > wrote: > > > Dianova has an excellent antibody for CD31 in mice for FFPE tissue > samples. > > > > Liz > > > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO > > Box 18592 Boulder, CO 80308 > > (303) 682-3949 office > > (303) 881-0763 cell > > (303) 682-9060 fax > > liz@premierlab.com > > > > Ship to address: > > > > Premier Laboratory, LLC > > 1567 Skyway Drive, Unit E > > Longmont, CO 80504 > > ________________________________________ > > From: histonet-bounces@lists.utsouthwestern.edu [ > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mesru T [ > turkekul@gmail.com] > > Sent: Wednesday, September 18, 2013 4:29 PM > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] ZInc formalin > > > > Dear Histonetters, > > > > I do not think there is a good CD31 (PECAM-1) antibody that will > > work on FFPE mouse tissues. If you have one please let me know. We > > use CD34 rat mono (Abcam) and Meca-32 Rat mono (Dev Hybridoma Bank > > Iowa Univ.) to > label > > endothelial cells of blood vessels in FFPE mouse tissues. > > There is a CD31 Rat mono from BD that will work of 10% NBF fixed, > > sucrose protected cryo embedded mouse tissues with antigen retrieval. > > The same CD31 Rat mono from BD will also work on Zinc Fixative from > > BD cat# 51-7538KZ ( it is a solution of ZnCl2 and maybe other zinc > > salts but no aldehyde) fixed paraffin embedded mouse tissues. The > > morphology of the section will be suboptimal due to this novel fixative. > > If anybody has any experince with this Zinc Fixative please would > > you > share? > > > > Regards, > > > > Mesruh Turkekul > > mskcc.org > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Mehmet Fatih BOZKURT, DVM, PhD Afyon Kocatepe University Faculty of Veterinary Medicine Department of Pathology 03030, ANS Campus Afyonkarahisar-TURKEY Tel: +902722281312-16173/16237 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tkngflght <@t> yahoo.com Thu Sep 19 09:55:26 2013 From: tkngflght <@t> yahoo.com (Cheryl) Date: Thu Sep 19 09:58:06 2013 Subject: [Histonet] Seeking refurb Leica 2235 Microtome Message-ID: <1379602526.79174.YahooMailNeo@web161901.mail.bf1.yahoo.com> Hi Guys- ? Looking?to purchase?a refurbished Leica 2235 Microtome with a low profile blade holder.?? Vendor responses welcome. ? Thank you! Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab by helping one GREAT?Tech at a time.? 281.852.9457?Office 800.756.3309?Phone & Fax? admin@fullstaff.org From Valerie.Hannen <@t> parrishmed.com Thu Sep 19 10:04:20 2013 From: Valerie.Hannen <@t> parrishmed.com (Hannen, Valerie) Date: Thu Sep 19 10:04:29 2013 Subject: [Histonet] Bunsen Burner Message-ID: <450B7A81EDA0C54E97C53D60F00776C3232B4BB657@isexstore03> Hi all.. We are having a discussion/ disagreement in our department as far as whether using a bunsen burner at the embedding center is against fire codes. What is the consensus?? Thanks, Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.hannen@parrishmed.com =================== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" =================== From mpence <@t> grhs.net Thu Sep 19 10:10:50 2013 From: mpence <@t> grhs.net (Mike Pence) Date: Thu Sep 19 10:11:11 2013 Subject: [Histonet] RE: Bunsen Burner In-Reply-To: <450B7A81EDA0C54E97C53D60F00776C3232B4BB657@isexstore03> References: <450B7A81EDA0C54E97C53D60F00776C3232B4BB657@isexstore03> Message-ID: No open flames! :( -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie Sent: Thursday, September 19, 2013 10:04 AM To: Histonet Post (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Bunsen Burner Hi all.. We are having a discussion/ disagreement in our department as far as whether using a bunsen burner at the embedding center is against fire codes. What is the consensus?? Thanks, Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.hannen@parrishmed.com ============= "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ============= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Sep 19 10:20:40 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Sep 19 10:20:44 2013 Subject: [Histonet] Bunsen Burner In-Reply-To: <450B7A81EDA0C54E97C53D60F00776C3232B4BB657@isexstore03> References: <450B7A81EDA0C54E97C53D60F00776C3232B4BB657@isexstore03> Message-ID: <1379604040.97660.YahooMailNeo@web163103.mail.bf1.yahoo.com> Nowadays an "open flame" (= Bunsen burner) in a histolab with lots of flammables is against most fire department regulations. I do not think those regulations specifically name "Bunsen burner", just "open flame". Ren? J. ________________________________ From: "Hannen, Valerie" To: "Histonet Post (histonet@lists.utsouthwestern.edu)" Sent: Thursday, September 19, 2013 11:04 AM Subject: [Histonet] Bunsen Burner Hi all.. We are having a discussion/ disagreement in our department as far as whether using a bunsen burner at the embedding center is against fire codes. What is the consensus?? Thanks, Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.hannen@parrishmed.com ====================================="This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" =====================================_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Erin.Martin <@t> ucsf.edu Thu Sep 19 10:31:45 2013 From: Erin.Martin <@t> ucsf.edu (Martin, Erin) Date: Thu Sep 19 10:31:59 2013 Subject: [Histonet] Re: Bunsen Burner Message-ID: <24B7B291CC88D04AB663958E77A1F59D182C94@ex09.net.ucsf.edu> No open flames. Erin Martin, Histology Supervisor UCSF Dermatopathology Service 415-353-7248 Confidentiality Notice The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential and/or priviledged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited. If you receive this in error, please contact the sender and delete the material from any computer. From Mark.Elliott <@t> hli.ubc.ca Thu Sep 19 10:35:18 2013 From: Mark.Elliott <@t> hli.ubc.ca (Mark Elliott) Date: Thu Sep 19 10:35:26 2013 Subject: [Histonet] Antibodies to Cathepsin proforms Message-ID: <523AB746020000D60006551D@mail.hli.ubc.ca> I hope everyone is gearing up for the NSH Meeting this weekend. Would love to be there but unfortunately can't make it this year. Hope you all have a great time. I have been asked if there are antibodies to the various Cathepsins which will differentiate between the proform and the active forms of the cathepsin. Zymography was suggested as the better way to go but was asked to see about the antibody sensitivity. Thanks Mark From billodonnell <@t> catholichealth.net Thu Sep 19 10:56:24 2013 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Thu Sep 19 10:56:37 2013 Subject: [Histonet] RE: Bunsen Burner In-Reply-To: <450B7A81EDA0C54E97C53D60F00776C3232B4BB657@isexstore03> References: <450B7A81EDA0C54E97C53D60F00776C3232B4BB657@isexstore03> Message-ID: No open flames - which makes it hard to light our cigars. (Just kidding.... but I remember when...) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie Sent: Thursday, September 19, 2013 10:04 AM To: Histonet Post (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Bunsen Burner Hi all.. We are having a discussion/ disagreement in our department as far as whether using a bunsen burner at the embedding center is against fire codes. What is the consensus?? Thanks, Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.hannen@parrishmed.com ============= "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ============= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. From lowenjeff <@t> hotmail.com Thu Sep 19 11:17:09 2013 From: lowenjeff <@t> hotmail.com (jeff lowen) Date: Thu Sep 19 11:17:18 2013 Subject: [Histonet] RE: Bunsen Burner In-Reply-To: References: <450B7A81EDA0C54E97C53D60F00776C3232B4BB657@isexstore03>, Message-ID: yeah and these darn gloves get in the way of drinking my coffee and enjoying my sticky bun. > From: billodonnell@catholichealth.net > To: Valerie.Hannen@parrishmed.com; histonet@lists.utsouthwestern.edu > Date: Thu, 19 Sep 2013 15:56:24 +0000 > CC: > Subject: [Histonet] RE: Bunsen Burner > > No open flames - which makes it hard to light our cigars. (Just kidding.... but I remember when...) > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie > Sent: Thursday, September 19, 2013 10:04 AM > To: Histonet Post (histonet@lists.utsouthwestern.edu) > Subject: [Histonet] Bunsen Burner > > Hi all.. > > We are having a discussion/ disagreement in our department as far as whether using a bunsen burner at the embedding center is against fire codes. > > What is the consensus?? > > > Thanks, > > Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) > Histology Section Chief > Parrish Medical Center > 951 N. Washington Ave. > Titusville, Florida 32976 > Phone:(321) 268-6333 ext. 7506 > Fax: (321) 268-6149 > valerie.hannen@parrishmed.com > > > ============= > "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" > ============= > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kim-lake <@t> uiowa.edu Thu Sep 19 11:49:08 2013 From: kim-lake <@t> uiowa.edu (Lake, Kim S) Date: Thu Sep 19 11:49:25 2013 Subject: [Histonet] Microwaving Sakura racks Message-ID: Last week I posted a question asking if Sakura autostainer racks held up in the microwave. As usual, I got some great responses from the histonet community, and I wanted to share with anyone who might be interested, and for the benefit of the archives. Thanks to everyone who responded! --I have done it but they don't do well they will melt and become very brittle . --You can microwave them. They don't tend to last as long, but where I used to work in Colorado we would dry certain slides in the microwave for around 5 minutes and still put them in the oven for an additional 5 minutes to make sure they were full dry. You can do a trial before going full speed ahead with it. I wouldn't recommend it all the time. We only did it on our morning special stain slides and if we were in a time crunch. It is still probably best to dry them in the oven. --In my lab we routinely microwave those racks in boiling citrate buffer for 20 minutes, so they seem to be indestructible! --If you refer to those brownish ones, they are made of hard plastic and can be microwaved. Remove the handles though. Kim Lake Laboratory Manager Oral Pathology Laboratory University of Iowa College of Dentistry Phone (319) 384 4433 Fax (319) 353 5569 From TWELLEN <@t> LHS.ORG Thu Sep 19 12:35:31 2013 From: TWELLEN <@t> LHS.ORG (Wellen, Terry D :GS Histology) Date: Thu Sep 19 12:35:38 2013 Subject: [Histonet] Venata Her2 on Ultra Message-ID: <5F46C87AD649864F9FA8273E8E31507A02739CA7@SWM2006.lhsnt.legacyhs> Is anybody doing Her2 on the Ventana Ultra Platform? I am having trouble optimizing to my Pathologists satisfaction. Terrence D. Wellen HT(ASCP) Technical Specialist Legacy Good Samaritan Hospital Portland, OR 503-413-8954 From b427297 <@t> aol.com Thu Sep 19 12:43:31 2013 From: b427297 <@t> aol.com (BILL O'CONNOR) Date: Thu Sep 19 12:43:36 2013 Subject: [Histonet] ZInc formalin In-Reply-To: <14E2C6176416974295479C64A11CB9AE019BB56FE177@SBS2K8.premierlab.local> References: <14E2C6176416974295479C64A11CB9AE019BB5702266@SBS2K8.premierlab.local> <14E2C6176416974295479C64A11CB9AE019BB56FE177@SBS2K8.premierlab.local> Message-ID: <8D08376E3B6229C-C74-FBB0@webmail-d285.sysops.aol.com> Santa Cruz had the only really good antibody for CD31 in mouse that worked on FFPE. Yes, Liz - their goat died. I remember them telling me that about 10 years ago. I was working on a project at the time where CD31 was so very important in murine xenografts. No one has every really been able to get another antibody that was as good as that one. They tried to clone the goat, but no mas. Jackie -----Original Message----- From: Elizabeth Chlipala To: Mehmet Fatih BOZKURT ; Mesruh Turkekul ; Histonet Sent: Thu, Sep 19, 2013 9:48 am Subject: RE: [Histonet] ZInc formalin Hello All Unless something has changed, this is how I remember it. That antibody used o work for mouse on FFPE tissue samples ( I had a lot that did work and it was reat, but that was years ago) and then the goat died or something like hat??????. From what I understand now the current lot numbers do not work on FPE tissues samples. Has something changed recently that this antibody will ork again in FFPE mouse tissues, if so I'm interested. That antibody worked on ultiple species quite well before. Thanks Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC aboratory Manager remier Laboratory, LLC O Box 18592 oulder, CO 80308 ork (303) 682-3949 ax (303) 682-9060 ell (303) 881-0763 iz@premierlab.com ww.premierlab.com Ship to address: 1567 Skyway Drive, Unit E ongmont, CO 80504 -----Original Message----- rom: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] n Behalf Of Mehmet Fatih BOZKURT ent: Wednesday, September 18, 2013 10:41 PM o: Mesruh Turkekul; Histonet@lists.utsouthwestern.edu ubject: Re: [Histonet] ZInc formalin Santa Cruz, SC-1506 is a good goat polyclonal antibody for mouse tissues. lso there is rabbit IgG that is coded SC-1506R. n Thu, Sep 19, 2013 at 4:32 AM, Mesruh Turkekul wrote: > Hi Liz, I checked the web site and the images of FFPE sections stained with CD31 from Dianova. It seems that it mostly stains large blood vessels. Which I have experienced with most CD31 Ab on the market. Does this ab stain all the capillaries in your experiments? Thanks again, Mesruh Sent from my iPad On Sep 18, 2013, at 7:51 PM, Elizabeth Chlipala wrote: > Dianova has an excellent antibody for CD31 in mice for FFPE tissue samples. > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO > Box 18592 Boulder, CO 80308 > (303) 682-3949 office > (303) 881-0763 cell > (303) 682-9060 fax > liz@premierlab.com > > Ship to address: > > Premier Laboratory, LLC > 1567 Skyway Drive, Unit E > Longmont, CO 80504 > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [ histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mesru T [ turkekul@gmail.com] > Sent: Wednesday, September 18, 2013 4:29 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] ZInc formalin > > Dear Histonetters, > > I do not think there is a good CD31 (PECAM-1) antibody that will > work on FFPE mouse tissues. If you have one please let me know. We > use CD34 rat mono (Abcam) and Meca-32 Rat mono (Dev Hybridoma Bank > Iowa Univ.) to label > endothelial cells of blood vessels in FFPE mouse tissues. > There is a CD31 Rat mono from BD that will work of 10% NBF fixed, > sucrose protected cryo embedded mouse tissues with antigen retrieval. > The same CD31 Rat mono from BD will also work on Zinc Fixative from > BD cat# 51-7538KZ ( it is a solution of ZnCl2 and maybe other zinc > salts but no aldehyde) fixed paraffin embedded mouse tissues. The > morphology of the section will be suboptimal due to this novel fixative. > If anybody has any experince with this Zinc Fixative please would > you share? > > Regards, > > Mesruh Turkekul > mskcc.org > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- ehmet Fatih BOZKURT, DVM, PhD fyon Kocatepe University aculty of Veterinary Medicine epartment of Pathology 3030, ANS Campus fyonkarahisar-TURKEY el: +902722281312-16173/16237 ______________________________________________ istonet mailing list istonet@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ istonet mailing list istonet@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet From patpxs <@t> gmail.com Thu Sep 19 12:58:09 2013 From: patpxs <@t> gmail.com (Paula Sicurello) Date: Thu Sep 19 12:58:14 2013 Subject: [Histonet] RE: Bunsen Burner In-Reply-To: References: <450B7A81EDA0C54E97C53D60F00776C3232B4BB657@isexstore03> Message-ID: No open flames allowed. Bill, If you use nitrile gloves, the sticky buns don't stick! -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 HIPAA Privacy Notification: This message and any accompanying documents are covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, and contain information intended for the specific individual (s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying or the taking of any action based on the contents of this information is strictly prohibited . If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. On Thu, Sep 19, 2013 at 12:17 PM, jeff lowen wrote: > yeah and these darn gloves get in the way of drinking my coffee and > enjoying my sticky bun. > > > From: billodonnell@catholichealth.net > > To: Valerie.Hannen@parrishmed.com; histonet@lists.utsouthwestern.edu > > Date: Thu, 19 Sep 2013 15:56:24 +0000 > > CC: > > Subject: [Histonet] RE: Bunsen Burner > > > > No open flames - which makes it hard to light our cigars. (Just > kidding.... but I remember when...) > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie > > Sent: Thursday, September 19, 2013 10:04 AM > > To: Histonet Post (histonet@lists.utsouthwestern.edu) > > Subject: [Histonet] Bunsen Burner > > > > Hi all.. > > > > We are having a discussion/ disagreement in our department as far as > whether using a bunsen burner at the embedding center is against fire codes. > > > > What is the consensus?? > > > > > > Thanks, > > > > Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) > > Histology Section Chief > > Parrish Medical Center > > 951 N. Washington Ave. > > Titusville, Florida 32976 > > Phone:(321) 268-6333 ext. 7506 > > Fax: (321) 268-6149 > > valerie.hannen@parrishmed.com > > > > > > ============= > > "This email is intended solely for the use of the individual to whom it > is addressed and may contain information that is privileged, confidential > or otherwise exempt from disclosure under applicable law. If the reader of > this email is not the intended recipient or the employee or agent > responsible for delivering the message to the intended recipient, you are > hereby notified that any dissemination, distribution, or copying of this > communication is strictly prohibited. If you have received this > communication in error, please immediately delete this message. Thank you" > > ============= > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > This electronic mail and any attached documents are intended solely for > the named addressee(s) and contain confidential information. If you are not > an addressee, or responsible for delivering this email to an addressee, you > have received this email in error and are notified that reading, copying, > or disclosing this email is prohibited. If you received this email in > error, immediately reply to the sender and delete the message completely > from your computer system. > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From lowenjeff <@t> hotmail.com Thu Sep 19 13:09:25 2013 From: lowenjeff <@t> hotmail.com (jeff lowen) Date: Thu Sep 19 13:09:31 2013 Subject: [Histonet] RE: Bunsen Burner In-Reply-To: References: <450B7A81EDA0C54E97C53D60F00776C3232B4BB657@isexstore03>, , , Message-ID: will hot coffee melt the nitrile? Date: Thu, 19 Sep 2013 13:58:09 -0400 Subject: Re: [Histonet] RE: Bunsen Burner From: patpxs@gmail.com To: lowenjeff@hotmail.com CC: billodonnell@catholichealth.net; valerie.hannen@parrishmed.com; histonet@lists.utsouthwestern.edu No open flames allowed. Bill, If you use nitrile gloves, the sticky buns don't stick! -- Paula Sicurello, HTL (ASCP)Supervisor, Clinical Electron Microscopy Laboratory Duke University Health SystemRm.#251M, Duke South, Green ZoneDurham, North Carolina 27710P: 919.684.2091 HIPAA Privacy Notification: This message and any accompanying documents are covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, and contain information intended for the specific individual (s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying or the taking of any action based on the contents of this information is strictly prohibited . If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. On Thu, Sep 19, 2013 at 12:17 PM, jeff lowen wrote: yeah and these darn gloves get in the way of drinking my coffee and enjoying my sticky bun. > From: billodonnell@catholichealth.net > To: Valerie.Hannen@parrishmed.com; histonet@lists.utsouthwestern.edu > Date: Thu, 19 Sep 2013 15:56:24 +0000 > CC: > Subject: [Histonet] RE: Bunsen Burner > > No open flames - which makes it hard to light our cigars. (Just kidding.... but I remember when...) > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie > Sent: Thursday, September 19, 2013 10:04 AM > To: Histonet Post (histonet@lists.utsouthwestern.edu) > Subject: [Histonet] Bunsen Burner > > Hi all.. > > We are having a discussion/ disagreement in our department as far as whether using a bunsen burner at the embedding center is against fire codes. > > What is the consensus?? > > > Thanks, > > Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) > Histology Section Chief > Parrish Medical Center > 951 N. Washington Ave. > Titusville, Florida 32976 > Phone:(321) 268-6333 ext. 7506 > Fax: (321) 268-6149 > valerie.hannen@parrishmed.com > > > ============= > "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" > ============= > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From turkekul <@t> gmail.com Thu Sep 19 13:10:56 2013 From: turkekul <@t> gmail.com (Mesru T) Date: Thu Sep 19 13:10:59 2013 Subject: [Histonet] ZInc formalin In-Reply-To: <8D08376E3B6229C-C74-FBB0@webmail-d285.sysops.aol.com> References: <14E2C6176416974295479C64A11CB9AE019BB5702266@SBS2K8.premierlab.local> <14E2C6176416974295479C64A11CB9AE019BB56FE177@SBS2K8.premierlab.local> <8D08376E3B6229C-C74-FBB0@webmail-d285.sysops.aol.com> Message-ID: Hi All, Actually we have tried the new lots of the dead goat antibody with Ultamap kit (polymer) on Ventana and worked pretty well. But the kit was so expensive and we did not continue. Mesru From madeathridge <@t> pastnashville.com Thu Sep 19 16:22:48 2013 From: madeathridge <@t> pastnashville.com (Mary Ann Deathridge) Date: Thu Sep 19 16:23:38 2013 Subject: [Histonet] Used histology equipment Message-ID: <6092483a$19e08623$3aa66978$@com> Hi Histonetters: I know a pathologist that is trying to find a microtome and tissue processor that is quite cheap for sale or donation He is trying help a mission lab in India acquire instruments. Thanks in advance. From chuckcabral11 <@t> yahoo.com Thu Sep 19 18:58:07 2013 From: chuckcabral11 <@t> yahoo.com (Chuck Cabral) Date: Thu Sep 19 18:58:12 2013 Subject: [Histonet] Bunsen Burner Message-ID: <1379635087.18241.YahooMailNeo@web161401.mail.bf1.yahoo.com> Oh wow we use alcohol lamps in our embedding stations. So we are breaking fire codes? We are in the SF Bay area. From lowenjeff <@t> hotmail.com Thu Sep 19 19:21:37 2013 From: lowenjeff <@t> hotmail.com (jeff lowen) Date: Thu Sep 19 19:21:41 2013 Subject: [Histonet] Bunsen Burner In-Reply-To: <1379635087.18241.YahooMailNeo@web161401.mail.bf1.yahoo.com> References: <1379635087.18241.YahooMailNeo@web161401.mail.bf1.yahoo.com> Message-ID: and I in San Diego, shhhh don't tell anybody > Date: Thu, 19 Sep 2013 16:58:07 -0700 > From: chuckcabral11@yahoo.com > To: histonet@lists.utsouthwestern.edu > Subject: re: [Histonet] Bunsen Burner > > Oh wow we use alcohol lamps in our embedding stations. So we are breaking fire codes? We are in the SF Bay area. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CDavis <@t> che-east.org Fri Sep 20 08:36:49 2013 From: CDavis <@t> che-east.org (Davis, Cassie) Date: Fri Sep 20 08:36:54 2013 Subject: [Histonet] RE: bunsen burner at the embedding center Message-ID: <08861B9CF6C7774E874635A4818AE37B013EA744F8@CHEXCMS01.one.ads.che.org> Hi Valerie, When I started in Histo in 90' everybody used the alcohol burners...Open flame concern became a concerned and the separate forcep warmers were purchase because the old embedding centers did not have the nice warmers like the new ones do. The last place I worked at had an old embedding center when I started but we weren't allowed open flames. Fortunately, we found an unused Bacteria Incinerator that Micro. wasn't using and used that until that embedding center died. That worked great! Cassandra Davis CDavis@che-east.org 302-575-8095 From: "Hannen, Valerie" To: "Histonet Post (histonet@lists.utsouthwestern.edu)" Sent: Thursday, September 19, 2013 11:04 AM Subject: [Histonet] Bunsen Burner Hi all.. We are having a discussion/ disagreement in our department as far as whether using a bunsen burner at the embedding center is against fire codes. What is the consensus?? Thanks, Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.hannen@parrishmed.com Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From jburch01 <@t> gmail.com Fri Sep 20 08:41:15 2013 From: jburch01 <@t> gmail.com (Jim Burchette) Date: Fri Sep 20 08:41:24 2013 Subject: [Histonet] RE: bunsen burner at the embedding center In-Reply-To: <08861B9CF6C7774E874635A4818AE37B013EA744F8@CHEXCMS01.one.ads.che.org> References: <08861B9CF6C7774E874635A4818AE37B013EA744F8@CHEXCMS01.one.ads.che.org> Message-ID: Back in the 70's we would boil metal base molds in water using a bunsen burner and a 3 legged ring stand. On Sep 20, 2013 9:37 AM, "Davis, Cassie" wrote: > Hi Valerie, > When I started in Histo in 90' everybody used the alcohol > burners...Open flame concern became a concerned and the separate forcep > warmers were purchase because the old embedding centers did not have the > nice warmers like the new ones do. The last place I worked at had an old > embedding center when I started but we weren't allowed open flames. > Fortunately, we found an unused Bacteria Incinerator that Micro. wasn't > using and used that until that embedding center died. That worked great! > > Cassandra Davis > CDavis@che-east.org > 302-575-8095 > > > From: "Hannen, Valerie" > To: "Histonet Post (histonet@lists.utsouthwestern.edu)" < > histonet@lists.utsouthwestern.edu> > Sent: Thursday, September 19, 2013 11:04 AM > Subject: [Histonet] Bunsen Burner > > > Hi all.. > > We are having a discussion/ disagreement in our department as far as > whether using a bunsen burner at the embedding center is against fire codes. > > What is the consensus?? > > > Thanks, > > Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) > Histology Section Chief > Parrish Medical Center > 951 N. Washington Ave. > Titusville, Florida 32976 > Phone:(321) 268-6333 ext. 7506 > Fax: (321) 268-6149 > valerie.hannen@parrishmed.com > > Confidentiality Notice: > This e-mail, including any attachments is the > property of Catholic Health East and is intended > for the sole use of the intended recipient(s). > It may contain information that is privileged and > confidential. Any unauthorized review, use, > disclosure, or distribution is prohibited. If you are > not the intended recipient, please delete this message, and > reply to the sender regarding the error in a separate email. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From ree3 <@t> leicester.ac.uk Fri Sep 20 08:45:32 2013 From: ree3 <@t> leicester.ac.uk (Edwards, Richard E.) Date: Fri Sep 20 08:46:05 2013 Subject: [Histonet] RE: bunsen burner at the embedding center In-Reply-To: References: <08861B9CF6C7774E874635A4818AE37B013EA744F8@CHEXCMS01.one.ads.che.org> Message-ID: <7722595275A4DD4FA225B92CDBF174A101B0D8D01AF1@EXC-MBX3.cfs.le.ac.uk> And sit around smoking as we mounted the sections from xylene, in an open lab.... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jim Burchette Sent: 20 September 2013 14:41 To: Davis, Cassie Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: bunsen burner at the embedding center Back in the 70's we would boil metal base molds in water using a bunsen burner and a 3 legged ring stand. On Sep 20, 2013 9:37 AM, "Davis, Cassie" wrote: > Hi Valerie, > When I started in Histo in 90' everybody used the alcohol > burners...Open flame concern became a concerned and the separate > forcep warmers were purchase because the old embedding centers did not > have the nice warmers like the new ones do. The last place I worked at > had an old embedding center when I started but we weren't allowed open flames. > Fortunately, we found an unused Bacteria Incinerator that Micro. > wasn't using and used that until that embedding center died. That worked great! > > Cassandra Davis > CDavis@che-east.org > 302-575-8095 > > > From: "Hannen, Valerie" > To: "Histonet Post (histonet@lists.utsouthwestern.edu)" < > histonet@lists.utsouthwestern.edu> > Sent: Thursday, September 19, 2013 11:04 AM > Subject: [Histonet] Bunsen Burner > > > Hi all.. > > We are having a discussion/ disagreement in our department as far as > whether using a bunsen burner at the embedding center is against fire codes. > > What is the consensus?? > > > Thanks, > > Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief > Parrish Medical Center > 951 N. Washington Ave. > Titusville, Florida 32976 > Phone:(321) 268-6333 ext. 7506 > Fax: (321) 268-6149 > valerie.hannen@parrishmed.com > > Confidentiality Notice: > This e-mail, including any attachments is the property of Catholic > Health East and is intended for the sole use of the intended > recipient(s). > It may contain information that is privileged and confidential. Any > unauthorized review, use, disclosure, or distribution is prohibited. > If you are not the intended recipient, please delete this message, and > reply to the sender regarding the error in a separate email. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Joyce.Weems <@t> emoryhealthcare.org Fri Sep 20 08:48:14 2013 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Fri Sep 20 08:48:34 2013 Subject: [Histonet] RE: bunsen burner at the embedding center In-Reply-To: <7722595275A4DD4FA225B92CDBF174A101B0D8D01AF1@EXC-MBX3.cfs.le.ac.uk> References: <08861B9CF6C7774E874635A4818AE37B013EA744F8@CHEXCMS01.one.ads.che.org> <7722595275A4DD4FA225B92CDBF174A101B0D8D01AF1@EXC-MBX3.cfs.le.ac.uk> Message-ID: With no ventilation.... Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Edwards, Richard E. Sent: Friday, September 20, 2013 9:46 AM To: 'Jim Burchette'; Davis, Cassie Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: bunsen burner at the embedding center And sit around smoking as we mounted the sections from xylene, in an open lab.... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jim Burchette Sent: 20 September 2013 14:41 To: Davis, Cassie Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: bunsen burner at the embedding center Back in the 70's we would boil metal base molds in water using a bunsen burner and a 3 legged ring stand. On Sep 20, 2013 9:37 AM, "Davis, Cassie" wrote: > Hi Valerie, > When I started in Histo in 90' everybody used the alcohol > burners...Open flame concern became a concerned and the separate > forcep warmers were purchase because the old embedding centers did not > have the nice warmers like the new ones do. The last place I worked at > had an old embedding center when I started but we weren't allowed open flames. > Fortunately, we found an unused Bacteria Incinerator that Micro. > wasn't using and used that until that embedding center died. That worked great! > > Cassandra Davis > CDavis@che-east.org > 302-575-8095 > > > From: "Hannen, Valerie" > To: "Histonet Post (histonet@lists.utsouthwestern.edu)" < > histonet@lists.utsouthwestern.edu> > Sent: Thursday, September 19, 2013 11:04 AM > Subject: [Histonet] Bunsen Burner > > > Hi all.. > > We are having a discussion/ disagreement in our department as far as > whether using a bunsen burner at the embedding center is against fire codes. > > What is the consensus?? > > > Thanks, > > Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief > Parrish Medical Center > 951 N. Washington Ave. > Titusville, Florida 32976 > Phone:(321) 268-6333 ext. 7506 > Fax: (321) 268-6149 > valerie.hannen@parrishmed.com > > Confidentiality Notice: > This e-mail, including any attachments is the property of Catholic > Health East and is intended for the sole use of the intended > recipient(s). > It may contain information that is privileged and confidential. Any > unauthorized review, use, disclosure, or distribution is prohibited. > If you are not the intended recipient, please delete this message, and > reply to the sender regarding the error in a separate email. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From Dolores_Fischer <@t> baxter.com Fri Sep 20 08:53:09 2013 From: Dolores_Fischer <@t> baxter.com (Fischer, Dolores) Date: Fri Sep 20 08:53:18 2013 Subject: [Histonet] RE: bunsen burner at the embedding center In-Reply-To: <7722595275A4DD4FA225B92CDBF174A101B0D8D01AF1@EXC-MBX3.cfs.le.ac.uk> References: <08861B9CF6C7774E874635A4818AE37B013EA744F8@CHEXCMS01.one.ads.che.org> <7722595275A4DD4FA225B92CDBF174A101B0D8D01AF1@EXC-MBX3.cfs.le.ac.uk> Message-ID: <7CA324454CDDCA40A56FC3C51923C0E4167E16EB@026-SN2MPN1-031.026d.mgd.msft.net> I almost walked into room with a 50 gallon drum of ether holding my lit cigarette...............caught myself at the last minute..........good ol' days! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Edwards, Richard E. Sent: Friday, September 20, 2013 8:46 AM To: 'Jim Burchette'; Davis, Cassie Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: bunsen burner at the embedding center And sit around smoking as we mounted the sections from xylene, in an open lab.... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jim Burchette Sent: 20 September 2013 14:41 To: Davis, Cassie Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: bunsen burner at the embedding center Back in the 70's we would boil metal base molds in water using a bunsen burner and a 3 legged ring stand. On Sep 20, 2013 9:37 AM, "Davis, Cassie" wrote: > Hi Valerie, > When I started in Histo in 90' everybody used the alcohol > burners...Open flame concern became a concerned and the separate > forcep warmers were purchase because the old embedding centers did not > have the nice warmers like the new ones do. The last place I worked at > had an old embedding center when I started but we weren't allowed open flames. > Fortunately, we found an unused Bacteria Incinerator that Micro. > wasn't using and used that until that embedding center died. That worked great! > > Cassandra Davis > CDavis@che-east.org > 302-575-8095 > > > From: "Hannen, Valerie" > To: "Histonet Post (histonet@lists.utsouthwestern.edu)" < > histonet@lists.utsouthwestern.edu> > Sent: Thursday, September 19, 2013 11:04 AM > Subject: [Histonet] Bunsen Burner > > > Hi all.. > > We are having a discussion/ disagreement in our department as far as > whether using a bunsen burner at the embedding center is against fire codes. > > What is the consensus?? > > > Thanks, > > Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief > Parrish Medical Center > 951 N. Washington Ave. > Titusville, Florida 32976 > Phone:(321) 268-6333 ext. 7506 > Fax: (321) 268-6149 > valerie.hannen@parrishmed.com > > Confidentiality Notice: > This e-mail, including any attachments is the property of Catholic > Health East and is intended for the sole use of the intended > recipient(s). > It may contain information that is privileged and confidential. Any > unauthorized review, use, disclosure, or distribution is prohibited. > If you are not the intended recipient, please delete this message, and > reply to the sender regarding the error in a separate email. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted is intended only for the person(s) or entity to which it is addressed and may contain confidential and/or legally privileged material. Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive privilege or confidentiality. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by entities other than the intended recipient is prohibited. If you receive this in error, please contact the sender and delete the material from any computer. For Translation: http://www.baxter.com/email_disclaimer From mthomas <@t> littonlab.com Fri Sep 20 08:53:47 2013 From: mthomas <@t> littonlab.com (Marla Thomas) Date: Fri Sep 20 08:54:18 2013 Subject: [Histonet] RE: bunsen burner at the embedding center In-Reply-To: References: <08861B9CF6C7774E874635A4818AE37B013EA744F8@CHEXCMS01.one.ads.che.org> <7722595275A4DD4FA225B92CDBF174A101B0D8D01AF1@EXC-MBX3.cfs.le.ac.uk> Message-ID: ...making solutions (mucicarmine, hematoxylin) and heating them over open flames. Gas jets on each counter top about 5 feet apart. Good ole days! Marla Thomas, HT(ASCP) Business Manager Litton Pathology Associates, PC 700 NW Hunter Dr. Blue Springs, MO 64015 Phone: 816-229-6449 Fax:816-874-4400 CONFIDENTIALITY NOTICE This message and any included attachments are from Litton Pathology Associates, P.C. and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call 816-229-6449 and ask for the HIPAA/Compliance Coordinator. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Friday, September 20, 2013 8:48 AM To: 'Edwards, Richard E.'; 'Jim Burchette'; Davis, Cassie Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: bunsen burner at the embedding center With no ventilation.... Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Edwards, Richard E. Sent: Friday, September 20, 2013 9:46 AM To: 'Jim Burchette'; Davis, Cassie Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: bunsen burner at the embedding center And sit around smoking as we mounted the sections from xylene, in an open lab.... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jim Burchette Sent: 20 September 2013 14:41 To: Davis, Cassie Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: bunsen burner at the embedding center Back in the 70's we would boil metal base molds in water using a bunsen burner and a 3 legged ring stand. On Sep 20, 2013 9:37 AM, "Davis, Cassie" wrote: > Hi Valerie, > When I started in Histo in 90' everybody used the alcohol > burners...Open flame concern became a concerned and the separate > forcep warmers were purchase because the old embedding centers did not > have the nice warmers like the new ones do. The last place I worked at > had an old embedding center when I started but we weren't allowed open flames. > Fortunately, we found an unused Bacteria Incinerator that Micro. > wasn't using and used that until that embedding center died. That worked great! > > Cassandra Davis > CDavis@che-east.org > 302-575-8095 > > > From: "Hannen, Valerie" > To: "Histonet Post (histonet@lists.utsouthwestern.edu)" < > histonet@lists.utsouthwestern.edu> > Sent: Thursday, September 19, 2013 11:04 AM > Subject: [Histonet] Bunsen Burner > > > Hi all.. > > We are having a discussion/ disagreement in our department as far as > whether using a bunsen burner at the embedding center is against fire codes. > > What is the consensus?? > > > Thanks, > > Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief > Parrish Medical Center > 951 N. Washington Ave. > Titusville, Florida 32976 > Phone:(321) 268-6333 ext. 7506 > Fax: (321) 268-6149 > valerie.hannen@parrishmed.com > > Confidentiality Notice: > This e-mail, including any attachments is the property of Catholic > Health East and is intended for the sole use of the intended > recipient(s). > It may contain information that is privileged and confidential. Any > unauthorized review, use, disclosure, or distribution is prohibited. > If you are not the intended recipient, please delete this message, and > reply to the sender regarding the error in a separate email. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Dolores_Fischer <@t> baxter.com Fri Sep 20 08:56:00 2013 From: Dolores_Fischer <@t> baxter.com (Fischer, Dolores) Date: Fri Sep 20 08:56:08 2013 Subject: [Histonet] RE: bunsen burner at the embedding center In-Reply-To: References: <08861B9CF6C7774E874635A4818AE37B013EA744F8@CHEXCMS01.one.ads.che.org> <7722595275A4DD4FA225B92CDBF174A101B0D8D01AF1@EXC-MBX3.cfs.le.ac.uk> Message-ID: <7CA324454CDDCA40A56FC3C51923C0E4167E1700@026-SN2MPN1-031.026d.mgd.msft.net> Yes, my first job, trimming tissue in a building referred to as the "barn". After working there for an extended period techs coming back to the main building would reek of formalin................ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Friday, September 20, 2013 8:48 AM To: 'Edwards, Richard E.'; 'Jim Burchette'; Davis, Cassie Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: bunsen burner at the embedding center With no ventilation.... Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Edwards, Richard E. Sent: Friday, September 20, 2013 9:46 AM To: 'Jim Burchette'; Davis, Cassie Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: bunsen burner at the embedding center And sit around smoking as we mounted the sections from xylene, in an open lab.... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jim Burchette Sent: 20 September 2013 14:41 To: Davis, Cassie Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: bunsen burner at the embedding center Back in the 70's we would boil metal base molds in water using a bunsen burner and a 3 legged ring stand. On Sep 20, 2013 9:37 AM, "Davis, Cassie" wrote: > Hi Valerie, > When I started in Histo in 90' everybody used the alcohol > burners...Open flame concern became a concerned and the separate > forcep warmers were purchase because the old embedding centers did not > have the nice warmers like the new ones do. The last place I worked at > had an old embedding center when I started but we weren't allowed open flames. > Fortunately, we found an unused Bacteria Incinerator that Micro. > wasn't using and used that until that embedding center died. That worked great! > > Cassandra Davis > CDavis@che-east.org > 302-575-8095 > > > From: "Hannen, Valerie" > To: "Histonet Post (histonet@lists.utsouthwestern.edu)" < > histonet@lists.utsouthwestern.edu> > Sent: Thursday, September 19, 2013 11:04 AM > Subject: [Histonet] Bunsen Burner > > > Hi all.. > > We are having a discussion/ disagreement in our department as far as > whether using a bunsen burner at the embedding center is against fire codes. > > What is the consensus?? > > > Thanks, > > Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief > Parrish Medical Center > 951 N. Washington Ave. > Titusville, Florida 32976 > Phone:(321) 268-6333 ext. 7506 > Fax: (321) 268-6149 > valerie.hannen@parrishmed.com > > Confidentiality Notice: > This e-mail, including any attachments is the property of Catholic > Health East and is intended for the sole use of the intended > recipient(s). > It may contain information that is privileged and confidential. Any > unauthorized review, use, disclosure, or distribution is prohibited. > If you are not the intended recipient, please delete this message, and > reply to the sender regarding the error in a separate email. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted is intended only for the person(s) or entity to which it is addressed and may contain confidential and/or legally privileged material. Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive privilege or confidentiality. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by entities other than the intended recipient is prohibited. If you receive this in error, please contact the sender and delete the material from any computer. For Translation: http://www.baxter.com/email_disclaimer From billodonnell <@t> catholichealth.net Fri Sep 20 09:09:01 2013 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Fri Sep 20 09:10:27 2013 Subject: [Histonet] RE: bunsen burner at the embedding center In-Reply-To: References: <08861B9CF6C7774E874635A4818AE37B013EA744F8@CHEXCMS01.one.ads.che.org> <7722595275A4DD4FA225B92CDBF174A101B0D8D01AF1@EXC-MBX3.cfs.le.ac.uk> Message-ID: .... and it was always snowing and I had to walk 19 miles to work uphill....both ways. . We had to stock our cryostat with fresh cut blocks of ice from the river 10 miles away. (uphill, both ways) We had to start our Bunsen burner with steel and flint and we used our fingers instead of forceps. G. Gorden Liddy was a wimp compared to us Kids today don't know how good they got it! (They also don't know who G. Gordon Liddy was but can instinctively Google it) - Just some Friday fun for what has turned into a fun thread. - Oh wait, I forgot, this forum is for professional questions - so- Can you please unsubscribe me? Have a great weekend! - Bill -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Friday, September 20, 2013 8:48 AM To: 'Edwards, Richard E.'; 'Jim Burchette'; Davis, Cassie Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: bunsen burner at the embedding center With no ventilation.... Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Edwards, Richard E. Sent: Friday, September 20, 2013 9:46 AM To: 'Jim Burchette'; Davis, Cassie Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: bunsen burner at the embedding center And sit around smoking as we mounted the sections from xylene, in an open lab.... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jim Burchette Sent: 20 September 2013 14:41 To: Davis, Cassie Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: bunsen burner at the embedding center Back in the 70's we would boil metal base molds in water using a bunsen burner and a 3 legged ring stand. On Sep 20, 2013 9:37 AM, "Davis, Cassie" wrote: > Hi Valerie, > When I started in Histo in 90' everybody used the alcohol > burners...Open flame concern became a concerned and the separate > forcep warmers were purchase because the old embedding centers did not > have the nice warmers like the new ones do. The last place I worked at > had an old embedding center when I started but we weren't allowed open flames. > Fortunately, we found an unused Bacteria Incinerator that Micro. > wasn't using and used that until that embedding center died. That worked great! > > Cassandra Davis > CDavis@che-east.org > 302-575-8095 > > > From: "Hannen, Valerie" > To: "Histonet Post (histonet@lists.utsouthwestern.edu)" < > histonet@lists.utsouthwestern.edu> > Sent: Thursday, September 19, 2013 11:04 AM > Subject: [Histonet] Bunsen Burner > > > Hi all.. > > We are having a discussion/ disagreement in our department as far as > whether using a bunsen burner at the embedding center is against fire codes. > > What is the consensus?? > > > Thanks, > > Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief > Parrish Medical Center > 951 N. Washington Ave. > Titusville, Florida 32976 > Phone:(321) 268-6333 ext. 7506 > Fax: (321) 268-6149 > valerie.hannen@parrishmed.com > > Confidentiality Notice: > This e-mail, including any attachments is the property of Catholic > Health East and is intended for the sole use of the intended > recipient(s). > It may contain information that is privileged and confidential. Any > unauthorized review, use, disclosure, or distribution is prohibited. > If you are not the intended recipient, please delete this message, and > reply to the sender regarding the error in a separate email. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. From paw555 <@t> yahoo.com Fri Sep 20 09:14:21 2013 From: paw555 <@t> yahoo.com (pam plumlee) Date: Fri Sep 20 09:14:37 2013 Subject: [Histonet] Digipath Slides Scanner Message-ID: <1379686461.49842.YahooMailNeo@web120903.mail.ne1.yahoo.com> Histonetters:? Hoping to get some input on the Digipath Slide Scanning system from some users.? Also, if anyone has one south of Los Angeles?and is willing to let me come evaluate it-even better!? Thanks, Pam ? Pamela A. Plumlee H.T. (ASCP) Lead Histologist Histologist?|? bioTheranostics, Inc. 9640 Towne Centre Drive, Suite 200?|? San Diego, CA 92121 T ?| ?(858) 587-5881 ext.3881 F ?|? (858) 587-5871 pam.plumlee@biotheranostics.com http://www.biotheranostics.com/ From lblazek <@t> digestivespecialists.com Fri Sep 20 09:16:04 2013 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Fri Sep 20 09:16:16 2013 Subject: [Histonet] RE: bunsen burner at the embedding center In-Reply-To: References: <08861B9CF6C7774E874635A4818AE37B013EA744F8@CHEXCMS01.one.ads.che.org> <7722595275A4DD4FA225B92CDBF174A101B0D8D01AF1@EXC-MBX3.cfs.le.ac.uk> Message-ID: <5A2BD13465E061429D6455C8D6B40E39165E403D92@IBMB7Exchange.digestivespecialists.com> Embedding center? We had to pour melted paraffin into metal grid molds. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Friday, September 20, 2013 10:09 AM To: Weems, Joyce K.; 'Edwards, Richard E.'; 'Jim Burchette'; Davis, Cassie Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: bunsen burner at the embedding center .... and it was always snowing and I had to walk 19 miles to work uphill....both ways. . We had to stock our cryostat with fresh cut blocks of ice from the river 10 miles away. (uphill, both ways) We had to start our Bunsen burner with steel and flint and we used our fingers instead of forceps. G. Gorden Liddy was a wimp compared to us Kids today don't know how good they got it! (They also don't know who G. Gordon Liddy was but can instinctively Google it) - Just some Friday fun for what has turned into a fun thread. - Oh wait, I forgot, this forum is for professional questions - so- Can you please unsubscribe me? Have a great weekend! - Bill -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Friday, September 20, 2013 8:48 AM To: 'Edwards, Richard E.'; 'Jim Burchette'; Davis, Cassie Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: bunsen burner at the embedding center With no ventilation.... Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Edwards, Richard E. Sent: Friday, September 20, 2013 9:46 AM To: 'Jim Burchette'; Davis, Cassie Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: bunsen burner at the embedding center And sit around smoking as we mounted the sections from xylene, in an open lab.... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jim Burchette Sent: 20 September 2013 14:41 To: Davis, Cassie Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: bunsen burner at the embedding center Back in the 70's we would boil metal base molds in water using a bunsen burner and a 3 legged ring stand. On Sep 20, 2013 9:37 AM, "Davis, Cassie" wrote: > Hi Valerie, > When I started in Histo in 90' everybody used the alcohol > burners...Open flame concern became a concerned and the separate > forcep warmers were purchase because the old embedding centers did not > have the nice warmers like the new ones do. The last place I worked at > had an old embedding center when I started but we weren't allowed open flames. > Fortunately, we found an unused Bacteria Incinerator that Micro. > wasn't using and used that until that embedding center died. That worked great! > > Cassandra Davis > CDavis@che-east.org > 302-575-8095 > > > From: "Hannen, Valerie" > To: "Histonet Post (histonet@lists.utsouthwestern.edu)" < > histonet@lists.utsouthwestern.edu> > Sent: Thursday, September 19, 2013 11:04 AM > Subject: [Histonet] Bunsen Burner > > > Hi all.. > > We are having a discussion/ disagreement in our department as far as > whether using a bunsen burner at the embedding center is against fire codes. > > What is the consensus?? > > > Thanks, > > Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief > Parrish Medical Center > 951 N. Washington Ave. > Titusville, Florida 32976 > Phone:(321) 268-6333 ext. 7506 > Fax: (321) 268-6149 > valerie.hannen@parrishmed.com > > Confidentiality Notice: > This e-mail, including any attachments is the property of Catholic > Health East and is intended for the sole use of the intended > recipient(s). > It may contain information that is privileged and confidential. Any > unauthorized review, use, disclosure, or distribution is prohibited. > If you are not the intended recipient, please delete this message, and > reply to the sender regarding the error in a separate email. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Dolores_Fischer <@t> baxter.com Fri Sep 20 09:17:52 2013 From: Dolores_Fischer <@t> baxter.com (Fischer, Dolores) Date: Fri Sep 20 09:18:04 2013 Subject: [Histonet] RE: bunsen burner at the embedding center In-Reply-To: References: <08861B9CF6C7774E874635A4818AE37B013EA744F8@CHEXCMS01.one.ads.che.org> <7722595275A4DD4FA225B92CDBF174A101B0D8D01AF1@EXC-MBX3.cfs.le.ac.uk> Message-ID: <7CA324454CDDCA40A56FC3C51923C0E4167E172C@026-SN2MPN1-031.026d.mgd.msft.net> Hmmmm......sounds like a histo survivor game in the making. Yes it's Friday and I'm not playing at Nationals, so a little Friday reminiscing is a good thing! Happy day everyone! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Friday, September 20, 2013 9:09 AM To: Weems, Joyce K.; 'Edwards, Richard E.'; 'Jim Burchette'; Davis, Cassie Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: bunsen burner at the embedding center .... and it was always snowing and I had to walk 19 miles to work uphill....both ways. . We had to stock our cryostat with fresh cut blocks of ice from the river 10 miles away. (uphill, both ways) We had to start our Bunsen burner with steel and flint and we used our fingers instead of forceps. G. Gorden Liddy was a wimp compared to us Kids today don't know how good they got it! (They also don't know who G. Gordon Liddy was but can instinctively Google it) - Just some Friday fun for what has turned into a fun thread. - Oh wait, I forgot, this forum is for professional questions - so- Can you please unsubscribe me? Have a great weekend! - Bill -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Friday, September 20, 2013 8:48 AM To: 'Edwards, Richard E.'; 'Jim Burchette'; Davis, Cassie Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: bunsen burner at the embedding center With no ventilation.... Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Edwards, Richard E. Sent: Friday, September 20, 2013 9:46 AM To: 'Jim Burchette'; Davis, Cassie Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: bunsen burner at the embedding center And sit around smoking as we mounted the sections from xylene, in an open lab.... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jim Burchette Sent: 20 September 2013 14:41 To: Davis, Cassie Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: bunsen burner at the embedding center Back in the 70's we would boil metal base molds in water using a bunsen burner and a 3 legged ring stand. On Sep 20, 2013 9:37 AM, "Davis, Cassie" wrote: > Hi Valerie, > When I started in Histo in 90' everybody used the alcohol > burners...Open flame concern became a concerned and the separate > forcep warmers were purchase because the old embedding centers did not > have the nice warmers like the new ones do. The last place I worked at > had an old embedding center when I started but we weren't allowed open flames. > Fortunately, we found an unused Bacteria Incinerator that Micro. > wasn't using and used that until that embedding center died. That worked great! > > Cassandra Davis > CDavis@che-east.org > 302-575-8095 > > > From: "Hannen, Valerie" > To: "Histonet Post (histonet@lists.utsouthwestern.edu)" < > histonet@lists.utsouthwestern.edu> > Sent: Thursday, September 19, 2013 11:04 AM > Subject: [Histonet] Bunsen Burner > > > Hi all.. > > We are having a discussion/ disagreement in our department as far as > whether using a bunsen burner at the embedding center is against fire codes. > > What is the consensus?? > > > Thanks, > > Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief > Parrish Medical Center > 951 N. Washington Ave. > Titusville, Florida 32976 > Phone:(321) 268-6333 ext. 7506 > Fax: (321) 268-6149 > valerie.hannen@parrishmed.com > > Confidentiality Notice: > This e-mail, including any attachments is the property of Catholic > Health East and is intended for the sole use of the intended > recipient(s). > It may contain information that is privileged and confidential. Any > unauthorized review, use, disclosure, or distribution is prohibited. > If you are not the intended recipient, please delete this message, and > reply to the sender regarding the error in a separate email. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted is intended only for the person(s) or entity to which it is addressed and may contain confidential and/or legally privileged material. Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive privilege or confidentiality. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by entities other than the intended recipient is prohibited. If you receive this in error, please contact the sender and delete the material from any computer. For Translation: http://www.baxter.com/email_disclaimer From anolan <@t> prometheushealthcare.com Fri Sep 20 09:18:09 2013 From: anolan <@t> prometheushealthcare.com (anolan@prometheushealthcare.com) Date: Fri Sep 20 09:18:10 2013 Subject: [Histonet] Histology Opportunity- Boston, MA Message-ID: <003d01ceb60c$3da4db00$b8ee9100$@prometheushealthcare.com> Hi all, I'm currently recruiting for several histology opportunities in Boston, MA. Please follow up with me directly with any questions or to apply. Anna Nolan Recruiter Prometheus Healthcare Direct Line 301-693-8908 Office 301-693-9057 Fax 301-368-2478 anolan @prometheushealthcare.com http://www.linkedin.com/pub/annelise-nolan/55/ba0/ab6 www.prometheushealthcare.com From BSullivan <@t> virtua.org Fri Sep 20 09:19:19 2013 From: BSullivan <@t> virtua.org (Sullivan, Beatrice) Date: Fri Sep 20 09:19:30 2013 Subject: [Histonet] RE: bunsen burner at the embedding center In-Reply-To: References: <08861B9CF6C7774E874635A4818AE37B013EA744F8@CHEXCMS01.one.ads.che.org> <7722595275A4DD4FA225B92CDBF174A101B0D8D01AF1@EXC-MBX3.cfs.le.ac.uk> Message-ID: <6932520047F7EE46B512E9801344F160380CA22D@ExchangeMB-1.Virtua.org> Toooooooooooooooooo funny !!!!!!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Friday, September 20, 2013 10:09 AM To: Weems, Joyce K.; 'Edwards, Richard E.'; 'Jim Burchette'; Davis, Cassie Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: bunsen burner at the embedding center .... and it was always snowing and I had to walk 19 miles to work uphill....both ways. . We had to stock our cryostat with fresh cut blocks of ice from the river 10 miles away. (uphill, both ways) We had to start our Bunsen burner with steel and flint and we used our fingers instead of forceps. G. Gorden Liddy was a wimp compared to us Kids today don't know how good they got it! (They also don't know who G. Gordon Liddy was but can instinctively Google it) - Just some Friday fun for what has turned into a fun thread. - Oh wait, I forgot, this forum is for professional questions - so- Can you please unsubscribe me? Have a great weekend! - Bill -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Friday, September 20, 2013 8:48 AM To: 'Edwards, Richard E.'; 'Jim Burchette'; Davis, Cassie Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: bunsen burner at the embedding center With no ventilation.... Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Edwards, Richard E. Sent: Friday, September 20, 2013 9:46 AM To: 'Jim Burchette'; Davis, Cassie Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: bunsen burner at the embedding center And sit around smoking as we mounted the sections from xylene, in an open lab.... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jim Burchette Sent: 20 September 2013 14:41 To: Davis, Cassie Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: bunsen burner at the embedding center Back in the 70's we would boil metal base molds in water using a bunsen burner and a 3 legged ring stand. On Sep 20, 2013 9:37 AM, "Davis, Cassie" wrote: > Hi Valerie, > When I started in Histo in 90' everybody used the alcohol > burners...Open flame concern became a concerned and the separate > forcep warmers were purchase because the old embedding centers did not > have the nice warmers like the new ones do. The last place I worked at > had an old embedding center when I started but we weren't allowed open flames. > Fortunately, we found an unused Bacteria Incinerator that Micro. > wasn't using and used that until that embedding center died. That worked great! > > Cassandra Davis > CDavis@che-east.org > 302-575-8095 > > > From: "Hannen, Valerie" > To: "Histonet Post (histonet@lists.utsouthwestern.edu)" < > histonet@lists.utsouthwestern.edu> > Sent: Thursday, September 19, 2013 11:04 AM > Subject: [Histonet] Bunsen Burner > > > Hi all.. > > We are having a discussion/ disagreement in our department as far as > whether using a bunsen burner at the embedding center is against fire codes. > > What is the consensus?? > > > Thanks, > > Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief > Parrish Medical Center > 951 N. Washington Ave. > Titusville, Florida 32976 > Phone:(321) 268-6333 ext. 7506 > Fax: (321) 268-6149 > valerie.hannen@parrishmed.com > > Confidentiality Notice: > This e-mail, including any attachments is the property of Catholic > Health East and is intended for the sole use of the intended > recipient(s). > It may contain information that is privileged and confidential. Any > unauthorized review, use, disclosure, or distribution is prohibited. > If you are not the intended recipient, please delete this message, and > reply to the sender regarding the error in a separate email. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message, and any included attachments, are from Virtua Health or its related affiliates and is intended only for the addressee(s). The information contained herein is privileged, proprietary or may include confidential information and/or protected patient health information. Any unauthorized review, forwarding, printing, copying, distributing, or otherwise disseminating or taking any action based on such information is strictly prohibited. If you have received this message in error, or have reason to believe you are not authorized to receive it, please delete this message promptly and notify the sender by e-mail with a copy to ISSECURITY@virtua.org. Thank you From ree3 <@t> leicester.ac.uk Fri Sep 20 09:20:06 2013 From: ree3 <@t> leicester.ac.uk (Edwards, Richard E.) Date: Fri Sep 20 09:20:19 2013 Subject: [Histonet] RE: bunsen burner at the embedding center In-Reply-To: References: <08861B9CF6C7774E874635A4818AE37B013EA744F8@CHEXCMS01.one.ads.che.org> <7722595275A4DD4FA225B92CDBF174A101B0D8D01AF1@EXC-MBX3.cfs.le.ac.uk> Message-ID: <7722595275A4DD4FA225B92CDBF174A101B0D8D01AF3@EXC-MBX3.cfs.le.ac.uk> Well we had to go down the blast furnace, grab a bit of steel and make it into a microtome knife before we were even allowed in the lab......................not to mention finding and cutting down a Haematoxylum campechianum tree before lunch.. -----Original Message----- From: O'Donnell, Bill [mailto:billodonnell@catholichealth.net] Sent: 20 September 2013 15:09 To: Weems, Joyce K.; 'Edwards, Richard E.'; 'Jim Burchette'; Davis, Cassie Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: bunsen burner at the embedding center .... and it was always snowing and I had to walk 19 miles to work uphill....both ways. . We had to stock our cryostat with fresh cut blocks of ice from the river 10 miles away. (uphill, both ways) We had to start our Bunsen burner with steel and flint and we used our fingers instead of forceps. G. Gorden Liddy was a wimp compared to us Kids today don't know how good they got it! (They also don't know who G. Gordon Liddy was but can instinctively Google it) - Just some Friday fun for what has turned into a fun thread. - Oh wait, I forgot, this forum is for professional questions - so- Can you please unsubscribe me? Have a great weekend! - Bill -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Friday, September 20, 2013 8:48 AM To: 'Edwards, Richard E.'; 'Jim Burchette'; Davis, Cassie Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: bunsen burner at the embedding center With no ventilation.... Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Edwards, Richard E. Sent: Friday, September 20, 2013 9:46 AM To: 'Jim Burchette'; Davis, Cassie Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: bunsen burner at the embedding center And sit around smoking as we mounted the sections from xylene, in an open lab.... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jim Burchette Sent: 20 September 2013 14:41 To: Davis, Cassie Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: bunsen burner at the embedding center Back in the 70's we would boil metal base molds in water using a bunsen burner and a 3 legged ring stand. On Sep 20, 2013 9:37 AM, "Davis, Cassie" wrote: > Hi Valerie, > When I started in Histo in 90' everybody used the alcohol > burners...Open flame concern became a concerned and the separate > forcep warmers were purchase because the old embedding centers did not > have the nice warmers like the new ones do. The last place I worked at > had an old embedding center when I started but we weren't allowed open flames. > Fortunately, we found an unused Bacteria Incinerator that Micro. > wasn't using and used that until that embedding center died. That worked great! > > Cassandra Davis > CDavis@che-east.org > 302-575-8095 > > > From: "Hannen, Valerie" > To: "Histonet Post (histonet@lists.utsouthwestern.edu)" < > histonet@lists.utsouthwestern.edu> > Sent: Thursday, September 19, 2013 11:04 AM > Subject: [Histonet] Bunsen Burner > > > Hi all.. > > We are having a discussion/ disagreement in our department as far as > whether using a bunsen burner at the embedding center is against fire codes. > > What is the consensus?? > > > Thanks, > > Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief > Parrish Medical Center > 951 N. Washington Ave. > Titusville, Florida 32976 > Phone:(321) 268-6333 ext. 7506 > Fax: (321) 268-6149 > valerie.hannen@parrishmed.com > > Confidentiality Notice: > This e-mail, including any attachments is the property of Catholic > Health East and is intended for the sole use of the intended > recipient(s). > It may contain information that is privileged and confidential. Any > unauthorized review, use, disclosure, or distribution is prohibited. > If you are not the intended recipient, please delete this message, and > reply to the sender regarding the error in a separate email. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. From Dolores_Fischer <@t> baxter.com Fri Sep 20 09:25:09 2013 From: Dolores_Fischer <@t> baxter.com (Fischer, Dolores) Date: Fri Sep 20 09:25:19 2013 Subject: [Histonet] RE: bunsen burner at the embedding center In-Reply-To: <7722595275A4DD4FA225B92CDBF174A101B0D8D01AF3@EXC-MBX3.cfs.le.ac.uk> References: <08861B9CF6C7774E874635A4818AE37B013EA744F8@CHEXCMS01.one.ads.che.org> <7722595275A4DD4FA225B92CDBF174A101B0D8D01AF1@EXC-MBX3.cfs.le.ac.uk> <7722595275A4DD4FA225B92CDBF174A101B0D8D01AF3@EXC-MBX3.cfs.le.ac.uk> Message-ID: <7CA324454CDDCA40A56FC3C51923C0E4167E1767@026-SN2MPN1-031.026d.mgd.msft.net> We had to go out in the fields and catch mice and rats before we could begin our studies...........(first job, before animal regulations) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Edwards, Richard E. Sent: Friday, September 20, 2013 9:20 AM To: 'O'Donnell, Bill'; Weems, Joyce K.; 'Edwards, Richard E.'; 'Jim Burchette'; Davis, Cassie Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: bunsen burner at the embedding center Well we had to go down the blast furnace, grab a bit of steel and make it into a microtome knife before we were even allowed in the lab......................not to mention finding and cutting down a Haematoxylum campechianum tree before lunch.. -----Original Message----- From: O'Donnell, Bill [mailto:billodonnell@catholichealth.net] Sent: 20 September 2013 15:09 To: Weems, Joyce K.; 'Edwards, Richard E.'; 'Jim Burchette'; Davis, Cassie Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: bunsen burner at the embedding center .... and it was always snowing and I had to walk 19 miles to work uphill....both ways. . We had to stock our cryostat with fresh cut blocks of ice from the river 10 miles away. (uphill, both ways) We had to start our Bunsen burner with steel and flint and we used our fingers instead of forceps. G. Gorden Liddy was a wimp compared to us Kids today don't know how good they got it! (They also don't know who G. Gordon Liddy was but can instinctively Google it) - Just some Friday fun for what has turned into a fun thread. - Oh wait, I forgot, this forum is for professional questions - so- Can you please unsubscribe me? Have a great weekend! - Bill -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Friday, September 20, 2013 8:48 AM To: 'Edwards, Richard E.'; 'Jim Burchette'; Davis, Cassie Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: bunsen burner at the embedding center With no ventilation.... Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Edwards, Richard E. Sent: Friday, September 20, 2013 9:46 AM To: 'Jim Burchette'; Davis, Cassie Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: bunsen burner at the embedding center And sit around smoking as we mounted the sections from xylene, in an open lab.... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jim Burchette Sent: 20 September 2013 14:41 To: Davis, Cassie Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: bunsen burner at the embedding center Back in the 70's we would boil metal base molds in water using a bunsen burner and a 3 legged ring stand. On Sep 20, 2013 9:37 AM, "Davis, Cassie" wrote: > Hi Valerie, > When I started in Histo in 90' everybody used the alcohol > burners...Open flame concern became a concerned and the separate > forcep warmers were purchase because the old embedding centers did not > have the nice warmers like the new ones do. The last place I worked at > had an old embedding center when I started but we weren't allowed open flames. > Fortunately, we found an unused Bacteria Incinerator that Micro. > wasn't using and used that until that embedding center died. That worked great! > > Cassandra Davis > CDavis@che-east.org > 302-575-8095 > > > From: "Hannen, Valerie" > To: "Histonet Post (histonet@lists.utsouthwestern.edu)" < > histonet@lists.utsouthwestern.edu> > Sent: Thursday, September 19, 2013 11:04 AM > Subject: [Histonet] Bunsen Burner > > > Hi all.. > > We are having a discussion/ disagreement in our department as far as > whether using a bunsen burner at the embedding center is against fire codes. > > What is the consensus?? > > > Thanks, > > Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief > Parrish Medical Center > 951 N. Washington Ave. > Titusville, Florida 32976 > Phone:(321) 268-6333 ext. 7506 > Fax: (321) 268-6149 > valerie.hannen@parrishmed.com > > Confidentiality Notice: > This e-mail, including any attachments is the property of Catholic > Health East and is intended for the sole use of the intended > recipient(s). > It may contain information that is privileged and confidential. Any > unauthorized review, use, disclosure, or distribution is prohibited. > If you are not the intended recipient, please delete this message, and > reply to the sender regarding the error in a separate email. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted is intended only for the person(s) or entity to which it is addressed and may contain confidential and/or legally privileged material. Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive privilege or confidentiality. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by entities other than the intended recipient is prohibited. If you receive this in error, please contact the sender and delete the material from any computer. For Translation: http://www.baxter.com/email_disclaimer From Joyce.Weems <@t> emoryhealthcare.org Fri Sep 20 09:28:29 2013 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Fri Sep 20 09:28:52 2013 Subject: [Histonet] RE: bunsen burner at the embedding center In-Reply-To: <7CA324454CDDCA40A56FC3C51923C0E4167E1767@026-SN2MPN1-031.026d.mgd.msft.net> References: <08861B9CF6C7774E874635A4818AE37B013EA744F8@CHEXCMS01.one.ads.che.org> <7722595275A4DD4FA225B92CDBF174A101B0D8D01AF1@EXC-MBX3.cfs.le.ac.uk> <7722595275A4DD4FA225B92CDBF174A101B0D8D01AF3@EXC-MBX3.cfs.le.ac.uk> <7CA324454CDDCA40A56FC3C51923C0E4167E1767@026-SN2MPN1-031.026d.mgd.msft.net> Message-ID: And then there was the time someone left the refrigerator door open and the frogs got loose.... Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fischer, Dolores Sent: Friday, September 20, 2013 10:25 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: bunsen burner at the embedding center We had to go out in the fields and catch mice and rats before we could begin our studies...........(first job, before animal regulations) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Edwards, Richard E. Sent: Friday, September 20, 2013 9:20 AM To: 'O'Donnell, Bill'; Weems, Joyce K.; 'Edwards, Richard E.'; 'Jim Burchette'; Davis, Cassie Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: bunsen burner at the embedding center Well we had to go down the blast furnace, grab a bit of steel and make it into a microtome knife before we were even allowed in the lab......................not to mention finding and cutting down a Haematoxylum campechianum tree before lunch.. -----Original Message----- From: O'Donnell, Bill [mailto:billodonnell@catholichealth.net] Sent: 20 September 2013 15:09 To: Weems, Joyce K.; 'Edwards, Richard E.'; 'Jim Burchette'; Davis, Cassie Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: bunsen burner at the embedding center .... and it was always snowing and I had to walk 19 miles to work uphill....both ways. . We had to stock our cryostat with fresh cut blocks of ice from the river 10 miles away. (uphill, both ways) We had to start our Bunsen burner with steel and flint and we used our fingers instead of forceps. G. Gorden Liddy was a wimp compared to us Kids today don't know how good they got it! (They also don't know who G. Gordon Liddy was but can instinctively Google it) - Just some Friday fun for what has turned into a fun thread. - Oh wait, I forgot, this forum is for professional questions - so- Can you please unsubscribe me? Have a great weekend! - Bill -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Friday, September 20, 2013 8:48 AM To: 'Edwards, Richard E.'; 'Jim Burchette'; Davis, Cassie Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: bunsen burner at the embedding center With no ventilation.... Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Edwards, Richard E. Sent: Friday, September 20, 2013 9:46 AM To: 'Jim Burchette'; Davis, Cassie Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: bunsen burner at the embedding center And sit around smoking as we mounted the sections from xylene, in an open lab.... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jim Burchette Sent: 20 September 2013 14:41 To: Davis, Cassie Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: bunsen burner at the embedding center Back in the 70's we would boil metal base molds in water using a bunsen burner and a 3 legged ring stand. On Sep 20, 2013 9:37 AM, "Davis, Cassie" wrote: > Hi Valerie, > When I started in Histo in 90' everybody used the alcohol > burners...Open flame concern became a concerned and the separate > forcep warmers were purchase because the old embedding centers did not > have the nice warmers like the new ones do. The last place I worked at > had an old embedding center when I started but we weren't allowed open flames. > Fortunately, we found an unused Bacteria Incinerator that Micro. > wasn't using and used that until that embedding center died. That worked great! > > Cassandra Davis > CDavis@che-east.org > 302-575-8095 > > > From: "Hannen, Valerie" > To: "Histonet Post (histonet@lists.utsouthwestern.edu)" < > histonet@lists.utsouthwestern.edu> > Sent: Thursday, September 19, 2013 11:04 AM > Subject: [Histonet] Bunsen Burner > > > Hi all.. > > We are having a discussion/ disagreement in our department as far as > whether using a bunsen burner at the embedding center is against fire codes. > > What is the consensus?? > > > Thanks, > > Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief > Parrish Medical Center > 951 N. Washington Ave. > Titusville, Florida 32976 > Phone:(321) 268-6333 ext. 7506 > Fax: (321) 268-6149 > valerie.hannen@parrishmed.com > > Confidentiality Notice: > This e-mail, including any attachments is the property of Catholic > Health East and is intended for the sole use of the intended > recipient(s). > It may contain information that is privileged and confidential. Any > unauthorized review, use, disclosure, or distribution is prohibited. > If you are not the intended recipient, please delete this message, and > reply to the sender regarding the error in a separate email. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted is intended only for the person(s) or entity to which it is addressed and may contain confidential and/or legally privileged material. Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive privilege or confidentiality. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by entities other than the intended recipient is prohibited. If you receive this in error, please contact the sender and delete the material from any computer. For Translation: http://www.baxter.com/email_disclaimer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Caroline.Pratt <@t> uphs.upenn.edu Fri Sep 20 09:34:38 2013 From: Caroline.Pratt <@t> uphs.upenn.edu (Pratt, Caroline) Date: Fri Sep 20 09:34:44 2013 Subject: [Histonet] Medicare Retractions Message-ID: Has anyone out there in an Office Lab or Independent Lab seen Medicare rejections or retractions lately on the TC portion of biopsies originating in practices that are billing Outpatient Hospital? I would appreciate anyone's input. Thanks! Caroline M. Pratt, MBA Practice Administrator Dermpath 3020 Market Street, Ste 201 Philadelphia, PA 19104 Phone 215-349-8178 Cell 610-800-1381 Fax 215-662-6150 The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From rsrichmond <@t> gmail.com Fri Sep 20 09:35:55 2013 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Fri Sep 20 09:35:58 2013 Subject: [Histonet] Re: bunsen burner at the embedding center Message-ID: I distinctly remember when I was a resident at Johns Hopkins in the late 1960s that the histotechs would smoke while staining and coverslipping without much ventilation. When I suggested to the chief technologist (who later died of smoking related disease at 65, but at least he hadn't set himself afire) that this wasn't such a good idea, he responded by stubbing out a lighted cigarette in a Stender dish full of xylene (apparently you can do this trick with gasoline also, but don't try it at home please). Buffering formalin was prohibited back then, and they removed the formalin pigment by passing the sections through a concentrated solution (20 or 30%) of picric acid in acetone. I'm glad he didn't try the cigarette trick in THAT Stender dish. Fast-forward nearly half a century, and in the three labs I'm working in I'm still grossing formalin-fixed tissue with minimal ventilation, but at least people aren't allowed to smoke in the lab any more. Bob Richmond Samurai Pathologist Maryville TN From TNMayer <@t> mdanderson.org Fri Sep 20 09:36:12 2013 From: TNMayer <@t> mdanderson.org (Mayer,Toysha N) Date: Fri Sep 20 09:36:16 2013 Subject: [Histonet] RE: Histonet Digest, Vol 118, Issue 35 In-Reply-To: <5269405b-b747-4b59-82a5-a8d3fe7571c2@D1PWPEXHUBCAS04.mdanderson.edu> References: <5269405b-b747-4b59-82a5-a8d3fe7571c2@D1PWPEXHUBCAS04.mdanderson.edu> Message-ID: <47E9B2C01DDDD94881EACD2DC44EBC8802B45E32@D1PWPEXMBX05.mdanderson.edu> Hey, I'm boiling some molds now on a hot plate, in hot soapy water, in a metal paraffin pot. Oh and the left over coffee from yesterday might melt the nitrile. Depending on who made it. Sincerely, Toysha N. Mayer, MBA, HT(ASCP) tnmayer@mdanderson.org Instructor/Education Coordinator Program in Histotechnology School of Health Professions MD Anderson Cancer Center 713-563.3481 Message: 4 Date: Thu, 19 Sep 2013 11:09:25 -0700 From: jeff lowen Subject: RE: [Histonet] RE: Bunsen Burner To: Paula Sicurello Cc: "Histonet Post \(histonet@lists.utsouthwestern.edu\)" , "Hannen, Valerie" Message-ID: Content-Type: text/plain; charset="iso-8859-1" will hot coffee melt the nitrile? Date: Thu, 19 Sep 2013 13:58:09 -0400 Subject: Re: [Histonet] RE: Bunsen Burner From: patpxs@gmail.com To: lowenjeff@hotmail.com CC: billodonnell@catholichealth.net; valerie.hannen@parrishmed.com; histonet@lists.utsouthwestern.edu Message: 10 Date: Fri, 20 Sep 2013 09:41:15 -0400 From: Jim Burchette Subject: Re: [Histonet] RE: bunsen burner at the embedding center To: "Davis, Cassie" Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Back in the 70's we would boil metal base molds in water using a bunsen burner and a 3 legged ring stand. On Sep 20, 2013 9:37 AM, "Davis, Cassie" wrote: > Hi Valerie, > When I started in Histo in 90' everybody used the alcohol > burners...Open flame concern became a concerned and the separate forcep > warmers were purchase because the old embedding centers did not have the > nice warmers like the new ones do. The last place I worked at had an old > embedding center when I started but we weren't allowed open flames. > Fortunately, we found an unused Bacteria Incinerator that Micro. wasn't > using and used that until that embedding center died. That worked great! > > Cassandra Davis > CDavis@che-east.org > 302-575-8095 From lowenjeff <@t> hotmail.com Fri Sep 20 09:39:25 2013 From: lowenjeff <@t> hotmail.com (jeff lowen) Date: Fri Sep 20 09:39:31 2013 Subject: [Histonet] RE: Histonet Digest, Vol 118, Issue 35 In-Reply-To: <47E9B2C01DDDD94881EACD2DC44EBC8802B45E32@D1PWPEXMBX05.mdanderson.edu> References: <5269405b-b747-4b59-82a5-a8d3fe7571c2@D1PWPEXHUBCAS04.mdanderson.edu>, <47E9B2C01DDDD94881EACD2DC44EBC8802B45E32@D1PWPEXMBX05.mdanderson.edu> Message-ID: depending on who made the coffee....or who made the gloves ? > From: TNMayer@mdanderson.org > To: histonet@lists.utsouthwestern.edu > Date: Fri, 20 Sep 2013 14:36:12 +0000 > Subject: [Histonet] RE: Histonet Digest, Vol 118, Issue 35 > > Hey, I'm boiling some molds now on a hot plate, in hot soapy water, in a metal paraffin pot. > > Oh and the left over coffee from yesterday might melt the nitrile. Depending on who made it. > > Sincerely, > > Toysha N. Mayer, MBA, HT(ASCP) > tnmayer@mdanderson.org > Instructor/Education Coordinator > Program in Histotechnology > School of Health Professions > MD Anderson Cancer Center > 713-563.3481 > > > Message: 4 > Date: Thu, 19 Sep 2013 11:09:25 -0700 > From: jeff lowen > Subject: RE: [Histonet] RE: Bunsen Burner > To: Paula Sicurello > Cc: "Histonet Post \(histonet@lists.utsouthwestern.edu\)" > , "Hannen, Valerie" > > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > will hot coffee melt the nitrile? > > Date: Thu, 19 Sep 2013 13:58:09 -0400 > Subject: Re: [Histonet] RE: Bunsen Burner > From: patpxs@gmail.com > To: lowenjeff@hotmail.com > CC: billodonnell@catholichealth.net; valerie.hannen@parrishmed.com; histonet@lists.utsouthwestern.edu > > Message: 10 > Date: Fri, 20 Sep 2013 09:41:15 -0400 > From: Jim Burchette > Subject: Re: [Histonet] RE: bunsen burner at the embedding center > To: "Davis, Cassie" > Cc: histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset=ISO-8859-1 > > Back in the 70's we would boil metal base molds in water using a bunsen > burner and a 3 legged ring stand. > On Sep 20, 2013 9:37 AM, "Davis, Cassie" wrote: > > > Hi Valerie, > > When I started in Histo in 90' everybody used the alcohol > > burners...Open flame concern became a concerned and the separate forcep > > warmers were purchase because the old embedding centers did not have the > > nice warmers like the new ones do. The last place I worked at had an old > > embedding center when I started but we weren't allowed open flames. > > Fortunately, we found an unused Bacteria Incinerator that Micro. wasn't > > using and used that until that embedding center died. That worked great! > > > > Cassandra Davis > > CDavis@che-east.org > > 302-575-8095 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mucram11 <@t> comcast.net Fri Sep 20 09:50:24 2013 From: mucram11 <@t> comcast.net (Pam Marcum) Date: Fri Sep 20 09:50:47 2013 Subject: [Histonet] Re: bunsen burner at the embedding center In-Reply-To: Message-ID: <327830906.1114116.1379688624620.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> I actually knew the person you are speaking of and it was his favorite trick.? We should be careful these younger people are using gloves for everything now to protect themselves.??W hen I started in Histology even the pathologists cleaned the paraffin off their hands with xylene and encouraged everyone to do the same .? We used so many things that are now not even allowed to be open on this planet and did know how dangerous or serious the possible issues could be over time.? Pam Marcum ----- Original Message ----- From: "Bob Richmond" To: "Histonet@lists.utsouthwestern.edu" Sent: Friday, September 20, 2013 9:35:55 AM Subject: [Histonet] Re: bunsen burner at the embedding center I distinctly remember when I was a resident at Johns Hopkins in the late 1960s that the histotechs would smoke while staining and coverslipping without much ventilation. When I suggested to the chief technologist (who later died of smoking related disease at 65, but at least he hadn't set himself afire) that this wasn't such a good idea, he responded by stubbing out a lighted cigarette in a Stender dish full of xylene (apparently you can do this trick with gasoline also, but don't try it at home please). Buffering formalin was prohibited back then, and they removed the formalin pigment by passing the sections through a concentrated solution (20 or 30%) of picric acid in acetone. I'm glad he didn't try the cigarette trick in THAT Stender dish. Fast-forward nearly half a century, and in the three labs I'm working in I'm still grossing formalin-fixed tissue with minimal ventilation, but at least people aren't allowed to smoke in the lab any more. Bob Richmond Samurai Pathologist Maryville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tpodawiltz <@t> lrgh.org Fri Sep 20 10:21:43 2013 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Fri Sep 20 10:21:50 2013 Subject: [Histonet] RE: bunsen burner at the embedding center In-Reply-To: References: <08861B9CF6C7774E874635A4818AE37B013EA744F8@CHEXCMS01.one.ads.che.org> <7722595275A4DD4FA225B92CDBF174A101B0D8D01AF1@EXC-MBX3.cfs.le.ac.uk> Message-ID: <38667E7FB77ECD4E91BFAEB8D98638632500FE660C@LRGHEXVS1.practice.lrgh.org> This thread reminds me of the time in the Navy, while assigned to the histology department full time we sometimes had to cover nights in hematology. I noticed the light on in the histology lab. I quietly opened the door to see one of the night chemistry techs smoking a joint while he stood next to our staining fume hood. I coughed and he tossed the joint into the sink. When asked by the chief if I was writing him up for getting stoned on duty I answered "No I am writing him up for being stupid, he could of blown both of us up" Tom -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Friday, September 20, 2013 10:09 AM To: Weems, Joyce K.; 'Edwards, Richard E.'; 'Jim Burchette'; Davis, Cassie Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: bunsen burner at the embedding center .... and it was always snowing and I had to walk 19 miles to work uphill....both ways. . We had to stock our cryostat with fresh cut blocks of ice from the river 10 miles away. (uphill, both ways) We had to start our Bunsen burner with steel and flint and we used our fingers instead of forceps. G. Gorden Liddy was a wimp compared to us Kids today don't know how good they got it! (They also don't know who G. Gordon Liddy was but can instinctively Google it) - Just some Friday fun for what has turned into a fun thread. - Oh wait, I forgot, this forum is for professional questions - so- Can you please unsubscribe me? Have a great weekend! - Bill -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Friday, September 20, 2013 8:48 AM To: 'Edwards, Richard E.'; 'Jim Burchette'; Davis, Cassie Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: bunsen burner at the embedding center With no ventilation.... Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Edwards, Richard E. Sent: Friday, September 20, 2013 9:46 AM To: 'Jim Burchette'; Davis, Cassie Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: bunsen burner at the embedding center And sit around smoking as we mounted the sections from xylene, in an open lab.... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jim Burchette Sent: 20 September 2013 14:41 To: Davis, Cassie Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: bunsen burner at the embedding center Back in the 70's we would boil metal base molds in water using a bunsen burner and a 3 legged ring stand. On Sep 20, 2013 9:37 AM, "Davis, Cassie" wrote: > Hi Valerie, > When I started in Histo in 90' everybody used the alcohol > burners...Open flame concern became a concerned and the separate > forcep warmers were purchase because the old embedding centers did not > have the nice warmers like the new ones do. The last place I worked at > had an old embedding center when I started but we weren't allowed open flames. > Fortunately, we found an unused Bacteria Incinerator that Micro. > wasn't using and used that until that embedding center died. That worked great! > > Cassandra Davis > CDavis@che-east.org > 302-575-8095 > > > From: "Hannen, Valerie" > To: "Histonet Post (histonet@lists.utsouthwestern.edu)" < > histonet@lists.utsouthwestern.edu> > Sent: Thursday, September 19, 2013 11:04 AM > Subject: [Histonet] Bunsen Burner > > > Hi all.. > > We are having a discussion/ disagreement in our department as far as > whether using a bunsen burner at the embedding center is against fire codes. > > What is the consensus?? > > > Thanks, > > Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief > Parrish Medical Center > 951 N. Washington Ave. > Titusville, Florida 32976 > Phone:(321) 268-6333 ext. 7506 > Fax: (321) 268-6149 > valerie.hannen@parrishmed.com > > Confidentiality Notice: > This e-mail, including any attachments is the property of Catholic > Health East and is intended for the sole use of the intended > recipient(s). > It may contain information that is privileged and confidential. Any > unauthorized review, use, disclosure, or distribution is prohibited. > If you are not the intended recipient, please delete this message, and > reply to the sender regarding the error in a separate email. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From lowenjeff <@t> hotmail.com Fri Sep 20 10:25:10 2013 From: lowenjeff <@t> hotmail.com (jeff lowen) Date: Fri Sep 20 10:25:22 2013 Subject: [Histonet] Re: bunsen burner at the embedding center In-Reply-To: <327830906.1114116.1379688624620.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> References: , <327830906.1114116.1379688624620.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Message-ID: yet here we are......how much of what they tell us is hyperbole? > Date: Fri, 20 Sep 2013 14:50:24 +0000 > From: mucram11@comcast.net > To: rsrichmond@gmail.com > Subject: Re: [Histonet] Re: bunsen burner at the embedding center > CC: histonet@lists.utsouthwestern.edu > > > > I actually knew the person you are speaking of and it was his favorite trick. We should be careful these younger people are using gloves for everything now to protect themselves. W hen I started in Histology even the pathologists cleaned the paraffin off their hands with xylene and encouraged everyone to do the same . We used so many things that are now not even allowed to be open on this planet and did know how dangerous or serious the possible issues could be over time. > > > Pam Marcum > > > > ----- Original Message ----- > From: "Bob Richmond" > To: "Histonet@lists.utsouthwestern.edu" > Sent: Friday, September 20, 2013 9:35:55 AM > Subject: [Histonet] Re: bunsen burner at the embedding center > > I distinctly remember when I was a resident at Johns Hopkins in the late > 1960s that the histotechs would smoke while staining and coverslipping > without much ventilation. When I suggested to the chief technologist (who > later died of smoking related disease at 65, but at least he hadn't set > himself afire) that this wasn't such a good idea, he responded by stubbing > out a lighted cigarette in a Stender dish full of xylene (apparently you > can do this trick with gasoline also, but don't try it at home please). > > Buffering formalin was prohibited back then, and they removed the formalin > pigment by passing the sections through a concentrated solution (20 or 30%) > of picric acid in acetone. I'm glad he didn't try the cigarette trick in > THAT Stender dish. > > Fast-forward nearly half a century, and in the three labs I'm working in > I'm still grossing formalin-fixed tissue with minimal ventilation, but at > least people aren't allowed to smoke in the lab any more. > > Bob Richmond > Samurai Pathologist > Maryville TN > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From patpxs <@t> gmail.com Fri Sep 20 10:44:50 2013 From: patpxs <@t> gmail.com (Paula Sicurello) Date: Fri Sep 20 10:44:55 2013 Subject: [Histonet] Re: bunsen burner at the embedding center In-Reply-To: References: <327830906.1114116.1379688624620.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Message-ID: I worked for the Navy Hospital in San Diego, way back in the day, and we did an extraction with fuming HCl (straight, 100% HCl for folks who didn't know what fuming meant). Several people would get nosebleeds every time they performed the extraction. I finally got one of those fans that looks like an airplane propellor in a cage and pried open the painted shut windows. I dared anyone to stop me, no one did! 1 was quite gutsy for a 22 year old. The Petty Officers had me make 10N NaOH and forgot to tell me how hot it got. I almost dropped it when I picked it up. Plus I had to swim to work, both ways in a rip tide-battleing sharks and jelly fish, while sloshing around in an earthquake! It is San Diego after all. Happy Friday! Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 HIPAA Privacy Notification: This message and any accompanying documents are covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, and contain information intended for the specific individual (s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying or the taking of any action based on the contents of this information is strictly prohibited . If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. On Fri, Sep 20, 2013 at 11:25 AM, jeff lowen wrote: > yet here we are......how much of what they tell us is hyperbole? > > > Date: Fri, 20 Sep 2013 14:50:24 +0000 > > From: mucram11@comcast.net > > To: rsrichmond@gmail.com > > Subject: Re: [Histonet] Re: bunsen burner at the embedding center > > CC: histonet@lists.utsouthwestern.edu > > > > > > > > I actually knew the person you are speaking of and it was his favorite > trick. We should be careful these younger people are using gloves for > everything now to protect themselves. W hen I started in Histology even > the pathologists cleaned the paraffin off their hands with xylene and > encouraged everyone to do the same . We used so many things that are now > not even allowed to be open on this planet and did know how dangerous or > serious the possible issues could be over time. > > > > > > Pam Marcum > > > > > > > > ----- Original Message ----- > > From: "Bob Richmond" > > To: "Histonet@lists.utsouthwestern.edu" < > histonet@lists.utsouthwestern.edu> > > Sent: Friday, September 20, 2013 9:35:55 AM > > Subject: [Histonet] Re: bunsen burner at the embedding center > > > > I distinctly remember when I was a resident at Johns Hopkins in the late > > 1960s that the histotechs would smoke while staining and coverslipping > > without much ventilation. When I suggested to the chief technologist (who > > later died of smoking related disease at 65, but at least he hadn't set > > himself afire) that this wasn't such a good idea, he responded by > stubbing > > out a lighted cigarette in a Stender dish full of xylene (apparently you > > can do this trick with gasoline also, but don't try it at home please). > > > > Buffering formalin was prohibited back then, and they removed the > formalin > > pigment by passing the sections through a concentrated solution (20 or > 30%) > > of picric acid in acetone. I'm glad he didn't try the cigarette trick in > > THAT Stender dish. > > > > Fast-forward nearly half a century, and in the three labs I'm working in > > I'm still grossing formalin-fixed tissue with minimal ventilation, but at > > least people aren't allowed to smoke in the lab any more. > > > > Bob Richmond > > Samurai Pathologist > > Maryville TN > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Vickroy.Jim <@t> mhsil.com Fri Sep 20 11:15:00 2013 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Fri Sep 20 11:15:05 2013 Subject: [Histonet] RE: EM instruments in SIU In-Reply-To: <523C6571.6070805@siumed.edu> References: <523C6571.6070805@siumed.edu> Message-ID: Here is the website for questions to other histotechs of which some do TEM. histonet@lists.utsouthwestern.edu As far as your instruments .......I am familiar with all except the TEM scope itself. I will return on Oct 8th and after that we can schedule a time for me to meet you over there. Have a good day. Jim From: Wei Du [mailto:wdu@siumed.edu] Sent: Friday, September 20, 2013 10:11 AM To: Vickroy, Jim Subject: EM instruments in SIU Hi Jim, I was nice talking to you and thank you for touring me your Pathological Lab. The EM instruments in our lab are listed as below: TEM instruments: Glass Knife Maker: model-LTB, 7800 Ultra Microtome for Thick Section: model---DuPont, MT-2 B Ultra Microtome for Thin Section: model--- RMC, MT-7 TEM: model---Hitachi, H-7000 with a digital camera Let me know if there are any other information you need. I will contact you after Oct 6. By the way, I met Dr. Gao yesterday when I was walking to parking lot and told him about the lab tour you had given and what we were going to do next. He highly recommended you. Wish you have good time in your vacation. Many many thanks, Wei ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From CDavis <@t> che-east.org Fri Sep 20 12:13:33 2013 From: CDavis <@t> che-east.org (Davis, Cassie) Date: Fri Sep 20 12:13:37 2013 Subject: [Histonet] The Good Old Days... Message-ID: <08861B9CF6C7774E874635A4818AE37B013EA744FA@CHEXCMS01.one.ads.che.org> I enjoy hearing sincere reminiscing...Even though us "kids" don't know how good we have it, some of us enjoy having an "old tech" beside us on the bench. I find weeding through the sarcasm can be profitable and in doing so have learned so much. What the old techs did on a daily basis, we only did in the practice lab and when the automated instruments and pre-made solutions that we have come to rely on fail, experience is so very valuable. Only by their blood, sweat and tears have we benefitted however, we have so far to go, let's do it together. Cassandra Davis CDavis@che-east.org 302-575-8095 Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From cls71877 <@t> gmail.com Fri Sep 20 12:39:40 2013 From: cls71877 <@t> gmail.com (Cristi Rigazio) Date: Fri Sep 20 12:39:51 2013 Subject: [Histonet] The Good Old Days... In-Reply-To: <08861B9CF6C7774E874635A4818AE37B013EA744FA@CHEXCMS01.one.ads.che.org> References: <08861B9CF6C7774E874635A4818AE37B013EA744FA@CHEXCMS01.one.ads.che.org> Message-ID: <8B7DDA80-17BB-4CA8-9877-B4648FA063FD@gmail.com> Hear hear! I agree and was just saying I love hearing the stories (although I am not young). Thanks all for sharing these memories and lessons! Sent from my iPhone On Sep 20, 2013, at 10:13 AM, "Davis, Cassie" wrote: > I enjoy hearing sincere reminiscing...Even though us "kids" don't know how good we have it, some of us enjoy having an "old tech" beside us on the bench. I find weeding through the sarcasm can be profitable and in doing so have learned so much. What the old techs did on a daily basis, we only did in the practice lab and when the automated instruments and pre-made solutions that we have come to rely on fail, experience is so very valuable. Only by their blood, sweat and tears have we benefitted however, we have so far to go, let's do it together. > > Cassandra Davis > CDavis@che-east.org > 302-575-8095 > > > > > > Confidentiality Notice: > This e-mail, including any attachments is the > property of Catholic Health East and is intended > for the sole use of the intended recipient(s). > It may contain information that is privileged and > confidential. Any unauthorized review, use, > disclosure, or distribution is prohibited. If you are > not the intended recipient, please delete this message, and > reply to the sender regarding the error in a separate email. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hfedor <@t> jhmi.edu Fri Sep 20 12:43:09 2013 From: hfedor <@t> jhmi.edu (Helen Fedor) Date: Fri Sep 20 12:43:29 2013 Subject: [Histonet] The Good Old Days... In-Reply-To: <8B7DDA80-17BB-4CA8-9877-B4648FA063FD@gmail.com> References: <08861B9CF6C7774E874635A4818AE37B013EA744FA@CHEXCMS01.one.ads.che.org> <8B7DDA80-17BB-4CA8-9877-B4648FA063FD@gmail.com> Message-ID: You can tell it is Friday. :) Helen -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cristi Rigazio Sent: Friday, September 20, 2013 1:40 PM To: Davis, Cassie Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] The Good Old Days... Hear hear! I agree and was just saying I love hearing the stories (although I am not young). Thanks all for sharing these memories and lessons! Sent from my iPhone On Sep 20, 2013, at 10:13 AM, "Davis, Cassie" wrote: > I enjoy hearing sincere reminiscing...Even though us "kids" don't know how good we have it, some of us enjoy having an "old tech" beside us on the bench. I find weeding through the sarcasm can be profitable and in doing so have learned so much. What the old techs did on a daily basis, we only did in the practice lab and when the automated instruments and pre-made solutions that we have come to rely on fail, experience is so very valuable. Only by their blood, sweat and tears have we benefitted however, we have so far to go, let's do it together. > > Cassandra Davis > CDavis@che-east.org > 302-575-8095 > > > > > > Confidentiality Notice: > This e-mail, including any attachments is the property of Catholic > Health East and is intended for the sole use of the intended > recipient(s). > It may contain information that is privileged and confidential. Any > unauthorized review, use, disclosure, or distribution is prohibited. > If you are not the intended recipient, please delete this message, and > reply to the sender regarding the error in a separate email. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kim.tournear <@t> yahoo.com Fri Sep 20 12:55:00 2013 From: kim.tournear <@t> yahoo.com (Kim Tournear) Date: Fri Sep 20 12:55:07 2013 Subject: [Histonet] Re: bunsen burner at the embedding center In-Reply-To: References: <327830906.1114116.1379688624620.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Message-ID: <28B87A1E-A7DE-40F1-B38A-9C39734BF5FB@yahoo.com> How many of us smoked and drank coffee at our microtomes? Or while doing autopsies? Those were the days and a lot of us are still around. Lol Sent from the iPhone of Kim Tournear ?? ? On Sep 20, 2013, at 8:44 AM, Paula Sicurello wrote: > I worked for the Navy Hospital in San Diego, way back in the day, and we > did an extraction with fuming HCl (straight, 100% HCl for folks who didn't > know what fuming meant). Several people would get nosebleeds every time > they performed the extraction. I finally got one of those fans that looks > like an airplane propellor in a cage and pried open the painted shut > windows. I dared anyone to stop me, no one did! 1 was quite gutsy for a > 22 year old. > The Petty Officers had me make 10N NaOH and forgot to tell me how hot it > got. I almost dropped it when I picked it up. > > Plus I had to swim to work, both ways in a rip tide-battleing sharks and > jelly fish, while sloshing around in an earthquake! It is San Diego after > all. > > Happy Friday! > > Paula > -- > Paula Sicurello, HTL (ASCP) > Supervisor, Clinical Electron Microscopy Laboratory > Duke University Health System > Rm.#251M, Duke South, Green Zone > Durham, North Carolina 27710 > P: 919.684.2091 > HIPAA Privacy Notification: This message and any accompanying documents are > covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, > and contain information intended for the specific individual (s) only. This > information is confidential. If you are not the intended recipient or an > agent responsible for delivering it to the intended recipient, you are > hereby notified that you have received this document in error and that any > review, dissemination, copying or the taking of any action based on the > contents of this information is strictly prohibited . If you have received > this communication in error, please notify us immediately by e-mail, and > delete the original message. > > > On Fri, Sep 20, 2013 at 11:25 AM, jeff lowen wrote: > >> yet here we are......how much of what they tell us is hyperbole? >> >>> Date: Fri, 20 Sep 2013 14:50:24 +0000 >>> From: mucram11@comcast.net >>> To: rsrichmond@gmail.com >>> Subject: Re: [Histonet] Re: bunsen burner at the embedding center >>> CC: histonet@lists.utsouthwestern.edu >>> >>> >>> >>> I actually knew the person you are speaking of and it was his favorite >> trick. We should be careful these younger people are using gloves for >> everything now to protect themselves. W hen I started in Histology even >> the pathologists cleaned the paraffin off their hands with xylene and >> encouraged everyone to do the same . We used so many things that are now >> not even allowed to be open on this planet and did know how dangerous or >> serious the possible issues could be over time. >>> >>> >>> Pam Marcum >>> >>> >>> >>> ----- Original Message ----- >>> From: "Bob Richmond" >>> To: "Histonet@lists.utsouthwestern.edu" < >> histonet@lists.utsouthwestern.edu> >>> Sent: Friday, September 20, 2013 9:35:55 AM >>> Subject: [Histonet] Re: bunsen burner at the embedding center >>> >>> I distinctly remember when I was a resident at Johns Hopkins in the late >>> 1960s that the histotechs would smoke while staining and coverslipping >>> without much ventilation. When I suggested to the chief technologist (who >>> later died of smoking related disease at 65, but at least he hadn't set >>> himself afire) that this wasn't such a good idea, he responded by >> stubbing >>> out a lighted cigarette in a Stender dish full of xylene (apparently you >>> can do this trick with gasoline also, but don't try it at home please). >>> >>> Buffering formalin was prohibited back then, and they removed the >> formalin >>> pigment by passing the sections through a concentrated solution (20 or >> 30%) >>> of picric acid in acetone. I'm glad he didn't try the cigarette trick in >>> THAT Stender dish. >>> >>> Fast-forward nearly half a century, and in the three labs I'm working in >>> I'm still grossing formalin-fixed tissue with minimal ventilation, but at >>> least people aren't allowed to smoke in the lab any more. >>> >>> Bob Richmond >>> Samurai Pathologist >>> Maryville TN >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vtobias <@t> uw.edu Fri Sep 20 13:00:31 2013 From: vtobias <@t> uw.edu (Victor A. Tobias) Date: Fri Sep 20 13:00:59 2013 Subject: [Histonet] The Good Old Days... In-Reply-To: <8B7DDA80-17BB-4CA8-9877-B4648FA063FD@gmail.com> References: <08861B9CF6C7774E874635A4818AE37B013EA744FA@CHEXCMS01.one.ads.che.org> <8B7DDA80-17BB-4CA8-9877-B4648FA063FD@gmail.com> Message-ID: Seems like no one has touched upon the fine art of knife sharpening. Sometimes I felt like an executioner sharpening my axe to the point of splitting a hair. Heaven forbid if you hit a staple. That part of the blade was no useless for days to weeks until the daily sharpening ground it out. Life is pretty good when you just pull out another blade from the plastic box. Victor Victor Tobias HT(ASCP) Clinical Applications Analyst Harborview Medical Center Dept of Pathology Room NJB 244 Ninth & Jefferson Seattle, WA 98104 vtobias@u.washington.edu 206-744-2735 206-744-8240 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cristi Rigazio Sent: Friday, September 20, 2013 10:40 AM To: Davis, Cassie Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] The Good Old Days... Hear hear! I agree and was just saying I love hearing the stories (although I am not young). Thanks all for sharing these memories and lessons! Sent from my iPhone On Sep 20, 2013, at 10:13 AM, "Davis, Cassie" wrote: > I enjoy hearing sincere reminiscing...Even though us "kids" don't know how good we have it, some of us enjoy having an "old tech" beside us on the bench. I find weeding through the sarcasm can be profitable and in doing so have learned so much. What the old techs did on a daily basis, we only did in the practice lab and when the automated instruments and pre-made solutions that we have come to rely on fail, experience is so very valuable. Only by their blood, sweat and tears have we benefitted however, we have so far to go, let's do it together. > > Cassandra Davis > CDavis@che-east.org > 302-575-8095 > > > > > > Confidentiality Notice: > This e-mail, including any attachments is the > property of Catholic Health East and is intended > for the sole use of the intended recipient(s). > It may contain information that is privileged and > confidential. Any unauthorized review, use, > disclosure, or distribution is prohibited. If you are > not the intended recipient, please delete this message, and > reply to the sender regarding the error in a separate email. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From estelahisto <@t> gmail.com Fri Sep 20 13:11:52 2013 From: estelahisto <@t> gmail.com (Estela Molina) Date: Fri Sep 20 13:11:55 2013 Subject: [Histonet] Unsuscribe Message-ID: From CDavis <@t> che-east.org Fri Sep 20 13:12:24 2013 From: CDavis <@t> che-east.org (Davis, Cassie) Date: Fri Sep 20 13:13:42 2013 Subject: [Histonet] RE: knife sharpening Message-ID: <08861B9CF6C7774E874635A4818AE37B013EA744FB@CHEXCMS01.one.ads.che.org> I thank God everyday the knife sharpening is over. The hair still stands up on the back of my neck at the memories of two senior techs who injured themselves while sharpening their knives. Cassandra Davis CDavis@che-east.org Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From lblazek <@t> digestivespecialists.com Fri Sep 20 13:14:42 2013 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Fri Sep 20 13:14:48 2013 Subject: [Histonet] Re: bunsen burner at the embedding center In-Reply-To: <28B87A1E-A7DE-40F1-B38A-9C39734BF5FB@yahoo.com> References: <327830906.1114116.1379688624620.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> <28B87A1E-A7DE-40F1-B38A-9C39734BF5FB@yahoo.com> Message-ID: <5A2BD13465E061429D6455C8D6B40E39165E403E7D@IBMB7Exchange.digestivespecialists.com> Lots of us and we survived! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Tournear Sent: Friday, September 20, 2013 1:55 PM To: Paula Sicurello Cc: Histonet@lists.utsouthwestern.edu; Bob Richmond Subject: Re: [Histonet] Re: bunsen burner at the embedding center How many of us smoked and drank coffee at our microtomes? Or while doing autopsies? Those were the days and a lot of us are still around. Lol Sent from the iPhone of Kim Tournear ?? ? On Sep 20, 2013, at 8:44 AM, Paula Sicurello wrote: > I worked for the Navy Hospital in San Diego, way back in the day, and > we did an extraction with fuming HCl (straight, 100% HCl for folks who > didn't know what fuming meant). Several people would get nosebleeds > every time they performed the extraction. I finally got one of those > fans that looks like an airplane propellor in a cage and pried open > the painted shut windows. I dared anyone to stop me, no one did! 1 > was quite gutsy for a > 22 year old. > The Petty Officers had me make 10N NaOH and forgot to tell me how hot > it got. I almost dropped it when I picked it up. > > Plus I had to swim to work, both ways in a rip tide-battleing sharks > and jelly fish, while sloshing around in an earthquake! It is San > Diego after all. > > Happy Friday! > > Paula > -- > Paula Sicurello, HTL (ASCP) > Supervisor, Clinical Electron Microscopy Laboratory Duke University > Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina > 27710 > P: 919.684.2091 > HIPAA Privacy Notification: This message and any accompanying > documents are covered by the Electronic Communications Privacy Act, 18 > U.S.C. 2510-2521, and contain information intended for the specific > individual (s) only. This information is confidential. If you are not > the intended recipient or an agent responsible for delivering it to > the intended recipient, you are hereby notified that you have received > this document in error and that any review, dissemination, copying or > the taking of any action based on the contents of this information is > strictly prohibited . If you have received this communication in > error, please notify us immediately by e-mail, and delete the original message. > > > On Fri, Sep 20, 2013 at 11:25 AM, jeff lowen wrote: > >> yet here we are......how much of what they tell us is hyperbole? >> >>> Date: Fri, 20 Sep 2013 14:50:24 +0000 >>> From: mucram11@comcast.net >>> To: rsrichmond@gmail.com >>> Subject: Re: [Histonet] Re: bunsen burner at the embedding center >>> CC: histonet@lists.utsouthwestern.edu >>> >>> >>> >>> I actually knew the person you are speaking of and it was his >>> favorite >> trick. We should be careful these younger people are using gloves >> for everything now to protect themselves. W hen I started in >> Histology even the pathologists cleaned the paraffin off their hands >> with xylene and encouraged everyone to do the same . We used so many >> things that are now not even allowed to be open on this planet and >> did know how dangerous or serious the possible issues could be over time. >>> >>> >>> Pam Marcum >>> >>> >>> >>> ----- Original Message ----- >>> From: "Bob Richmond" >>> To: "Histonet@lists.utsouthwestern.edu" < >> histonet@lists.utsouthwestern.edu> >>> Sent: Friday, September 20, 2013 9:35:55 AM >>> Subject: [Histonet] Re: bunsen burner at the embedding center >>> >>> I distinctly remember when I was a resident at Johns Hopkins in the >>> late 1960s that the histotechs would smoke while staining and >>> coverslipping without much ventilation. When I suggested to the >>> chief technologist (who later died of smoking related disease at 65, >>> but at least he hadn't set himself afire) that this wasn't such a >>> good idea, he responded by >> stubbing >>> out a lighted cigarette in a Stender dish full of xylene (apparently >>> you can do this trick with gasoline also, but don't try it at home please). >>> >>> Buffering formalin was prohibited back then, and they removed the >> formalin >>> pigment by passing the sections through a concentrated solution (20 >>> or >> 30%) >>> of picric acid in acetone. I'm glad he didn't try the cigarette >>> trick in THAT Stender dish. >>> >>> Fast-forward nearly half a century, and in the three labs I'm >>> working in I'm still grossing formalin-fixed tissue with minimal >>> ventilation, but at least people aren't allowed to smoke in the lab any more. >>> >>> Bob Richmond >>> Samurai Pathologist >>> Maryville TN >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek <@t> digestivespecialists.com Fri Sep 20 13:15:20 2013 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Fri Sep 20 13:15:27 2013 Subject: [Histonet] The Good Old Days... In-Reply-To: References: <08861B9CF6C7774E874635A4818AE37B013EA744FA@CHEXCMS01.one.ads.che.org> <8B7DDA80-17BB-4CA8-9877-B4648FA063FD@gmail.com> Message-ID: <5A2BD13465E061429D6455C8D6B40E39165E403E7E@IBMB7Exchange.digestivespecialists.com> How many can strop a knife? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victor A. Tobias Sent: Friday, September 20, 2013 2:01 PM To: Cristi Rigazio; Davis, Cassie Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] The Good Old Days... Seems like no one has touched upon the fine art of knife sharpening. Sometimes I felt like an executioner sharpening my axe to the point of splitting a hair. Heaven forbid if you hit a staple. That part of the blade was no useless for days to weeks until the daily sharpening ground it out. Life is pretty good when you just pull out another blade from the plastic box. Victor Victor Tobias HT(ASCP) Clinical Applications Analyst Harborview Medical Center Dept of Pathology Room NJB 244 Ninth & Jefferson Seattle, WA 98104 vtobias@u.washington.edu 206-744-2735 206-744-8240 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cristi Rigazio Sent: Friday, September 20, 2013 10:40 AM To: Davis, Cassie Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] The Good Old Days... Hear hear! I agree and was just saying I love hearing the stories (although I am not young). Thanks all for sharing these memories and lessons! Sent from my iPhone On Sep 20, 2013, at 10:13 AM, "Davis, Cassie" wrote: > I enjoy hearing sincere reminiscing...Even though us "kids" don't know how good we have it, some of us enjoy having an "old tech" beside us on the bench. I find weeding through the sarcasm can be profitable and in doing so have learned so much. What the old techs did on a daily basis, we only did in the practice lab and when the automated instruments and pre-made solutions that we have come to rely on fail, experience is so very valuable. Only by their blood, sweat and tears have we benefitted however, we have so far to go, let's do it together. > > Cassandra Davis > CDavis@che-east.org > 302-575-8095 > > > > > > Confidentiality Notice: > This e-mail, including any attachments is the property of Catholic > Health East and is intended for the sole use of the intended > recipient(s). > It may contain information that is privileged and confidential. Any > unauthorized review, use, disclosure, or distribution is prohibited. > If you are not the intended recipient, please delete this message, and > reply to the sender regarding the error in a separate email. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mtitford <@t> aol.com Fri Sep 20 13:20:33 2013 From: mtitford <@t> aol.com (mtitford@aol.com) Date: Fri Sep 20 13:20:38 2013 Subject: [Histonet] More about the good old days.. Message-ID: <8D084453AD6E037-1D80-391C@webmail-d278.sysops.aol.com> And the stuff we poured down the sink without a second thought.... From PAMarcum <@t> uams.edu Fri Sep 20 13:22:30 2013 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Fri Sep 20 13:22:56 2013 Subject: [Histonet] The Good Old Days... In-Reply-To: <5A2BD13465E061429D6455C8D6B40E39165E403E7E@IBMB7Exchange.digestivespecialists.com> References: <08861B9CF6C7774E874635A4818AE37B013EA744FA@CHEXCMS01.one.ads.che.org> <8B7DDA80-17BB-4CA8-9877-B4648FA063FD@gmail.com> <5A2BD13465E061429D6455C8D6B40E39165E403E7E@IBMB7Exchange.digestivespecialists.com> Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA32D315A21E@Mail2Node2.ad.uams.edu> I still can and learned from someone older than me years ago to save you any good cotton denim possible. If you stretch taut and gently run the edge of a sharpened knife over it, the material will remove fine grit and further smooth the edge. I can still remember the first person I saw drop a knife off the knife sharpener and become the luckiest person alive. She tried to catch it as a reflex and luckily caught on the heel not the edge. We were all screaming drop it and jump back. Pam -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Friday, September 20, 2013 1:15 PM To: Victor A. Tobias; Cristi Rigazio; Davis, Cassie Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] The Good Old Days... How many can strop a knife? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victor A. Tobias Sent: Friday, September 20, 2013 2:01 PM To: Cristi Rigazio; Davis, Cassie Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] The Good Old Days... Seems like no one has touched upon the fine art of knife sharpening. Sometimes I felt like an executioner sharpening my axe to the point of splitting a hair. Heaven forbid if you hit a staple. That part of the blade was no useless for days to weeks until the daily sharpening ground it out. Life is pretty good when you just pull out another blade from the plastic box. Victor Victor Tobias HT(ASCP) Clinical Applications Analyst Harborview Medical Center Dept of Pathology Room NJB 244 Ninth & Jefferson Seattle, WA 98104 vtobias@u.washington.edu 206-744-2735 206-744-8240 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cristi Rigazio Sent: Friday, September 20, 2013 10:40 AM To: Davis, Cassie Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] The Good Old Days... Hear hear! I agree and was just saying I love hearing the stories (although I am not young). Thanks all for sharing these memories and lessons! Sent from my iPhone On Sep 20, 2013, at 10:13 AM, "Davis, Cassie" wrote: > I enjoy hearing sincere reminiscing...Even though us "kids" don't know how good we have it, some of us enjoy having an "old tech" beside us on the bench. I find weeding through the sarcasm can be profitable and in doing so have learned so much. What the old techs did on a daily basis, we only did in the practice lab and when the automated instruments and pre-made solutions that we have come to rely on fail, experience is so very valuable. Only by their blood, sweat and tears have we benefitted however, we have so far to go, let's do it together. > > Cassandra Davis > CDavis@che-east.org > 302-575-8095 > > > > > > Confidentiality Notice: > This e-mail, including any attachments is the property of Catholic > Health East and is intended for the sole use of the intended > recipient(s). > It may contain information that is privileged and confidential. Any > unauthorized review, use, disclosure, or distribution is prohibited. > If you are not the intended recipient, please delete this message, and > reply to the sender regarding the error in a separate email. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From mthomas <@t> littonlab.com Fri Sep 20 13:27:45 2013 From: mthomas <@t> littonlab.com (Marla Thomas) Date: Fri Sep 20 13:28:24 2013 Subject: [Histonet] The Good Old Days... In-Reply-To: <5A2BD13465E061429D6455C8D6B40E39165E403E7E@IBMB7Exchange.digestivespecialists.com> References: <08861B9CF6C7774E874635A4818AE37B013EA744FA@CHEXCMS01.one.ads.che.org> <8B7DDA80-17BB-4CA8-9877-B4648FA063FD@gmail.com> <5A2BD13465E061429D6455C8D6B40E39165E403E7E@IBMB7Exchange.digestivespecialists.com> Message-ID: <7eca1cd1d06645e484bdd00cb73c4f95@BLUPR07MB178.namprd07.prod.outlook.com> Hone and Strap!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Friday, September 20, 2013 1:15 PM To: Victor A. Tobias; Cristi Rigazio; Davis, Cassie Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] The Good Old Days... How many can strop a knife? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victor A. Tobias Sent: Friday, September 20, 2013 2:01 PM To: Cristi Rigazio; Davis, Cassie Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] The Good Old Days... Seems like no one has touched upon the fine art of knife sharpening. Sometimes I felt like an executioner sharpening my axe to the point of splitting a hair. Heaven forbid if you hit a staple. That part of the blade was no useless for days to weeks until the daily sharpening ground it out. Life is pretty good when you just pull out another blade from the plastic box. Victor Victor Tobias HT(ASCP) Clinical Applications Analyst Harborview Medical Center Dept of Pathology Room NJB 244 Ninth & Jefferson Seattle, WA 98104 vtobias@u.washington.edu 206-744-2735 206-744-8240 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cristi Rigazio Sent: Friday, September 20, 2013 10:40 AM To: Davis, Cassie Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] The Good Old Days... Hear hear! I agree and was just saying I love hearing the stories (although I am not young). Thanks all for sharing these memories and lessons! Sent from my iPhone On Sep 20, 2013, at 10:13 AM, "Davis, Cassie" wrote: > I enjoy hearing sincere reminiscing...Even though us "kids" don't know how good we have it, some of us enjoy having an "old tech" beside us on the bench. I find weeding through the sarcasm can be profitable and in doing so have learned so much. What the old techs did on a daily basis, we only did in the practice lab and when the automated instruments and pre-made solutions that we have come to rely on fail, experience is so very valuable. Only by their blood, sweat and tears have we benefitted however, we have so far to go, let's do it together. > > Cassandra Davis > CDavis@che-east.org > 302-575-8095 > > > > > > Confidentiality Notice: > This e-mail, including any attachments is the property of Catholic > Health East and is intended for the sole use of the intended > recipient(s). > It may contain information that is privileged and confidential. Any > unauthorized review, use, disclosure, or distribution is prohibited. > If you are not the intended recipient, please delete this message, and > reply to the sender regarding the error in a separate email. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TGoins <@t> mt.gov Fri Sep 20 13:33:21 2013 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Fri Sep 20 13:33:32 2013 Subject: [Histonet] The Good Old Days... In-Reply-To: <5A2BD13465E061429D6455C8D6B40E39165E403E7E@IBMB7Exchange.digestivespecialists.com> References: <08861B9CF6C7774E874635A4818AE37B013EA744FA@CHEXCMS01.one.ads.che.org> <8B7DDA80-17BB-4CA8-9877-B4648FA063FD@gmail.com> <5A2BD13465E061429D6455C8D6B40E39165E403E7E@IBMB7Exchange.digestivespecialists.com> Message-ID: I've stropped blades until they looked like scythes - and I got pretty good at adjusting those three screws to line up my block on the microtome. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Friday, September 20, 2013 12:15 PM To: Victor A. Tobias; Cristi Rigazio; Davis, Cassie Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] The Good Old Days... How many can strop a knife? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victor A. Tobias Sent: Friday, September 20, 2013 2:01 PM To: Cristi Rigazio; Davis, Cassie Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] The Good Old Days... Seems like no one has touched upon the fine art of knife sharpening. Sometimes I felt like an executioner sharpening my axe to the point of splitting a hair. Heaven forbid if you hit a staple. That part of the blade was no useless for days to weeks until the daily sharpening ground it out. Life is pretty good when you just pull out another blade from the plastic box. Victor Victor Tobias HT(ASCP) Clinical Applications Analyst Harborview Medical Center Dept of Pathology Room NJB 244 Ninth & Jefferson Seattle, WA 98104 vtobias@u.washington.edu 206-744-2735 206-744-8240 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cristi Rigazio Sent: Friday, September 20, 2013 10:40 AM To: Davis, Cassie Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] The Good Old Days... Hear hear! I agree and was just saying I love hearing the stories (although I am not young). Thanks all for sharing these memories and lessons! Sent from my iPhone On Sep 20, 2013, at 10:13 AM, "Davis, Cassie" wrote: > I enjoy hearing sincere reminiscing...Even though us "kids" don't know how good we have it, some of us enjoy having an "old tech" beside us on the bench. I find weeding through the sarcasm can be profitable and in doing so have learned so much. What the old techs did on a daily basis, we only did in the practice lab and when the automated instruments and pre-made solutions that we have come to rely on fail, experience is so very valuable. Only by their blood, sweat and tears have we benefitted however, we have so far to go, let's do it together. > > Cassandra Davis > CDavis@che-east.org > 302-575-8095 > > > > > > Confidentiality Notice: > This e-mail, including any attachments is the property of Catholic > Health East and is intended for the sole use of the intended > recipient(s). > It may contain information that is privileged and confidential. Any > unauthorized review, use, disclosure, or distribution is prohibited. > If you are not the intended recipient, please delete this message, and > reply to the sender regarding the error in a separate email. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vtobias <@t> uw.edu Fri Sep 20 13:43:47 2013 From: vtobias <@t> uw.edu (Victor A. Tobias) Date: Fri Sep 20 13:44:34 2013 Subject: [Histonet] More about the good old days.. In-Reply-To: <8D084453AD6E037-1D80-391C@webmail-d278.sysops.aol.com> References: <8D084453AD6E037-1D80-391C@webmail-d278.sysops.aol.com> Message-ID: I gave plenty of thought to the ETOH going down the drain. Victor Tobias HT(ASCP) Clinical Applications Analyst Harborview Medical Center Dept of Pathology Room NJB 244 Ninth & Jefferson Seattle, WA 98104 vtobias@u.washington.edu 206-744-2735 206-744-8240 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of mtitford@aol.com Sent: Friday, September 20, 2013 11:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] More about the good old days.. And the stuff we poured down the sink without a second thought.... _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From patpxs <@t> gmail.com Fri Sep 20 14:09:51 2013 From: patpxs <@t> gmail.com (Paula Sicurello) Date: Fri Sep 20 14:09:55 2013 Subject: [Histonet] More about the good old days.. In-Reply-To: References: <8D084453AD6E037-1D80-391C@webmail-d278.sysops.aol.com> Message-ID: I had a co-worker who would drink CocaCola mixed with the sealed, glass flask 100% ETOH every Friday. -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 HIPAA Privacy Notification: This message and any accompanying documents are covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, and contain information intended for the specific individual (s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying or the taking of any action based on the contents of this information is strictly prohibited . If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. On Fri, Sep 20, 2013 at 2:43 PM, Victor A. Tobias wrote: > I gave plenty of thought to the ETOH going down the drain. > > Victor Tobias HT(ASCP) > Clinical Applications Analyst > Harborview Medical Center > Dept of Pathology Room NJB 244 > Ninth & Jefferson > Seattle, WA 98104 > vtobias@u.washington.edu > 206-744-2735 > 206-744-8240 Fax > ================================================= > Privileged, confidential or patient identifiable information may be > contained in this message. This information is meant only for the use > of the intended recipients. If you are not the intended recipient, or > if the message has been addressed to you in error, do not read, > disclose, reproduce, distribute, disseminate or otherwise use this > transmission. Instead, please notify the sender by reply e-mail, and > then destroy all copies of the message and any attachments. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of mtitford@aol.com > Sent: Friday, September 20, 2013 11:21 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] More about the good old days.. > > And the stuff we poured down the sink without a second thought.... > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From JWatson <@t> gnf.org Fri Sep 20 14:20:19 2013 From: JWatson <@t> gnf.org (James Watson) Date: Fri Sep 20 14:20:24 2013 Subject: [Histonet] The Good Old Days... In-Reply-To: References: <08861B9CF6C7774E874635A4818AE37B013EA744FA@CHEXCMS01.one.ads.che.org> <8B7DDA80-17BB-4CA8-9877-B4648FA063FD@gmail.com> <5A2BD13465E061429D6455C8D6B40E39165E403E7E@IBMB7Exchange.digestivespecialists.com> Message-ID: OK 1. Making formalin and Grossing with an old metal table fan to blow the formalin fumes away. 2. Finding the technicon hung up in the morning with the samples dried out. 3. Figuring out how to reprocess the tissue 4. Pan embedding and having the paper labels float away from the samples in the pan. a. Bunsen burner needed 5. Big kitchen knife heated over a Bunsen burner to make blocks 6. Soaking re-useable metal cassettes in a bucket of xylene. (next to where you were pan embedding) 7. Sharpening a knife on multiple stones then stropping on leather then cloth. 8. Over tightening the microtome clamp on the paraffin block and having to re-embed because the block broke. 9. Making staining solutions (some of us still do this). a. Making Schiffs regent without a hood b. Cresyl violet stain powder floating around the room, watch where you put your hands c. Silver nitrate spilled on the counter where you just put your hand then wiped your face. 10. Staining slides in open staining dishes with no fume hood. 11. Wiping the whole room down with xylene to clean the wax up. 12. Autopsies in the afternoon, 13. Whole human brains soaking in the sink in 5 gallons of 20% formalin overnight. Oh boy, really miss them good old days. Life was easier then! Hey Teri, When are we going to get our new coverslipper in? We were spoiled by the demo unit. Jamie James Watson HT? ASCP GNF? Genomics Institute of the Novartis Research Foundation Tel??? 858-332-4647 Fax?? 858-812-1915 jwatson@gnf.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Goins, Tresa Sent: Friday, September 20, 2013 11:33 AM To: Blazek, Linda; Victor A. Tobias; Cristi Rigazio; Davis, Cassie Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] The Good Old Days... I've stropped blades until they looked like scythes - and I got pretty good at adjusting those three screws to line up my block on the microtome. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Friday, September 20, 2013 12:15 PM To: Victor A. Tobias; Cristi Rigazio; Davis, Cassie Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] The Good Old Days... How many can strop a knife? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victor A. Tobias Sent: Friday, September 20, 2013 2:01 PM To: Cristi Rigazio; Davis, Cassie Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] The Good Old Days... Seems like no one has touched upon the fine art of knife sharpening. Sometimes I felt like an executioner sharpening my axe to the point of splitting a hair. Heaven forbid if you hit a staple. That part of the blade was no useless for days to weeks until the daily sharpening ground it out. Life is pretty good when you just pull out another blade from the plastic box. Victor Victor Tobias HT(ASCP) Clinical Applications Analyst Harborview Medical Center Dept of Pathology Room NJB 244 Ninth & Jefferson Seattle, WA 98104 vtobias@u.washington.edu 206-744-2735 206-744-8240 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cristi Rigazio Sent: Friday, September 20, 2013 10:40 AM To: Davis, Cassie Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] The Good Old Days... Hear hear! I agree and was just saying I love hearing the stories (although I am not young). Thanks all for sharing these memories and lessons! Sent from my iPhone On Sep 20, 2013, at 10:13 AM, "Davis, Cassie" wrote: > I enjoy hearing sincere reminiscing...Even though us "kids" don't know how good we have it, some of us enjoy having an "old tech" beside us on the bench. I find weeding through the sarcasm can be profitable and in doing so have learned so much. What the old techs did on a daily basis, we only did in the practice lab and when the automated instruments and pre-made solutions that we have come to rely on fail, experience is so very valuable. Only by their blood, sweat and tears have we benefitted however, we have so far to go, let's do it together. > > Cassandra Davis > CDavis@che-east.org > 302-575-8095 > > > > > > Confidentiality Notice: > This e-mail, including any attachments is the property of Catholic > Health East and is intended for the sole use of the intended > recipient(s). > It may contain information that is privileged and confidential. Any > unauthorized review, use, disclosure, or distribution is prohibited. > If you are not the intended recipient, please delete this message, and > reply to the sender regarding the error in a separate email. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek <@t> digestivespecialists.com Fri Sep 20 14:24:00 2013 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Fri Sep 20 14:24:05 2013 Subject: [Histonet] The Good Old Days... In-Reply-To: References: <08861B9CF6C7774E874635A4818AE37B013EA744FA@CHEXCMS01.one.ads.che.org> <8B7DDA80-17BB-4CA8-9877-B4648FA063FD@gmail.com> <5A2BD13465E061429D6455C8D6B40E39165E403E7E@IBMB7Exchange.digestivespecialists.com> Message-ID: <5A2BD13465E061429D6455C8D6B40E39165E403EC3@IBMB7Exchange.digestivespecialists.com> One has been missed! Doing gross without gloves. There was a pathologist that claimed to be able to feel the lymph nodes much better without gloves. -----Original Message----- From: James Watson [mailto:JWatson@gnf.org] Sent: Friday, September 20, 2013 3:20 PM To: 'Goins, Tresa'; Blazek, Linda; Victor A. Tobias; Cristi Rigazio; Davis, Cassie Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] The Good Old Days... OK 1. Making formalin and Grossing with an old metal table fan to blow the formalin fumes away. 2. Finding the technicon hung up in the morning with the samples dried out. 3. Figuring out how to reprocess the tissue 4. Pan embedding and having the paper labels float away from the samples in the pan. a. Bunsen burner needed 5. Big kitchen knife heated over a Bunsen burner to make blocks 6. Soaking re-useable metal cassettes in a bucket of xylene. (next to where you were pan embedding) 7. Sharpening a knife on multiple stones then stropping on leather then cloth. 8. Over tightening the microtome clamp on the paraffin block and having to re-embed because the block broke. 9. Making staining solutions (some of us still do this). a. Making Schiffs regent without a hood b. Cresyl violet stain powder floating around the room, watch where you put your hands c. Silver nitrate spilled on the counter where you just put your hand then wiped your face. 10. Staining slides in open staining dishes with no fume hood. 11. Wiping the whole room down with xylene to clean the wax up. 12. Autopsies in the afternoon, 13. Whole human brains soaking in the sink in 5 gallons of 20% formalin overnight. Oh boy, really miss them good old days. Life was easier then! Hey Teri, When are we going to get our new coverslipper in? We were spoiled by the demo unit. Jamie James Watson HT? ASCP GNF? Genomics Institute of the Novartis Research Foundation Tel??? 858-332-4647 Fax?? 858-812-1915 jwatson@gnf.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Goins, Tresa Sent: Friday, September 20, 2013 11:33 AM To: Blazek, Linda; Victor A. Tobias; Cristi Rigazio; Davis, Cassie Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] The Good Old Days... I've stropped blades until they looked like scythes - and I got pretty good at adjusting those three screws to line up my block on the microtome. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Friday, September 20, 2013 12:15 PM To: Victor A. Tobias; Cristi Rigazio; Davis, Cassie Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] The Good Old Days... How many can strop a knife? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victor A. Tobias Sent: Friday, September 20, 2013 2:01 PM To: Cristi Rigazio; Davis, Cassie Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] The Good Old Days... Seems like no one has touched upon the fine art of knife sharpening. Sometimes I felt like an executioner sharpening my axe to the point of splitting a hair. Heaven forbid if you hit a staple. That part of the blade was no useless for days to weeks until the daily sharpening ground it out. Life is pretty good when you just pull out another blade from the plastic box. Victor Victor Tobias HT(ASCP) Clinical Applications Analyst Harborview Medical Center Dept of Pathology Room NJB 244 Ninth & Jefferson Seattle, WA 98104 vtobias@u.washington.edu 206-744-2735 206-744-8240 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cristi Rigazio Sent: Friday, September 20, 2013 10:40 AM To: Davis, Cassie Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] The Good Old Days... Hear hear! I agree and was just saying I love hearing the stories (although I am not young). Thanks all for sharing these memories and lessons! Sent from my iPhone On Sep 20, 2013, at 10:13 AM, "Davis, Cassie" wrote: > I enjoy hearing sincere reminiscing...Even though us "kids" don't know how good we have it, some of us enjoy having an "old tech" beside us on the bench. I find weeding through the sarcasm can be profitable and in doing so have learned so much. What the old techs did on a daily basis, we only did in the practice lab and when the automated instruments and pre-made solutions that we have come to rely on fail, experience is so very valuable. Only by their blood, sweat and tears have we benefitted however, we have so far to go, let's do it together. > > Cassandra Davis > CDavis@che-east.org > 302-575-8095 > > > > > > Confidentiality Notice: > This e-mail, including any attachments is the property of Catholic > Health East and is intended for the sole use of the intended > recipient(s). > It may contain information that is privileged and confidential. Any > unauthorized review, use, disclosure, or distribution is prohibited. > If you are not the intended recipient, please delete this message, and > reply to the sender regarding the error in a separate email. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From billodonnell <@t> catholichealth.net Fri Sep 20 14:26:31 2013 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Fri Sep 20 14:27:07 2013 Subject: [Histonet] The Good Old Days... In-Reply-To: <5A2BD13465E061429D6455C8D6B40E39165E403E7E@IBMB7Exchange.digestivespecialists.com> References: <08861B9CF6C7774E874635A4818AE37B013EA744FA@CHEXCMS01.one.ads.che.org> <8B7DDA80-17BB-4CA8-9877-B4648FA063FD@gmail.com> <5A2BD13465E061429D6455C8D6B40E39165E403E7E@IBMB7Exchange.digestivespecialists.com> Message-ID: I can! I can! And still strop my straight razor every day as well as all the grossing knives and my kitchen knives as often as needed. (Different strops) - Bill -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Friday, September 20, 2013 1:15 PM To: Victor A. Tobias; Cristi Rigazio; Davis, Cassie Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] The Good Old Days... How many can strop a knife? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victor A. Tobias Sent: Friday, September 20, 2013 2:01 PM To: Cristi Rigazio; Davis, Cassie Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] The Good Old Days... Seems like no one has touched upon the fine art of knife sharpening. Sometimes I felt like an executioner sharpening my axe to the point of splitting a hair. Heaven forbid if you hit a staple. That part of the blade was no useless for days to weeks until the daily sharpening ground it out. Life is pretty good when you just pull out another blade from the plastic box. Victor Victor Tobias HT(ASCP) Clinical Applications Analyst Harborview Medical Center Dept of Pathology Room NJB 244 Ninth & Jefferson Seattle, WA 98104 vtobias@u.washington.edu 206-744-2735 206-744-8240 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cristi Rigazio Sent: Friday, September 20, 2013 10:40 AM To: Davis, Cassie Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] The Good Old Days... Hear hear! I agree and was just saying I love hearing the stories (although I am not young). Thanks all for sharing these memories and lessons! Sent from my iPhone On Sep 20, 2013, at 10:13 AM, "Davis, Cassie" wrote: > I enjoy hearing sincere reminiscing...Even though us "kids" don't know how good we have it, some of us enjoy having an "old tech" beside us on the bench. I find weeding through the sarcasm can be profitable and in doing so have learned so much. What the old techs did on a daily basis, we only did in the practice lab and when the automated instruments and pre-made solutions that we have come to rely on fail, experience is so very valuable. Only by their blood, sweat and tears have we benefitted however, we have so far to go, let's do it together. > > Cassandra Davis > CDavis@che-east.org > 302-575-8095 > > > > > > Confidentiality Notice: > This e-mail, including any attachments is the property of Catholic > Health East and is intended for the sole use of the intended > recipient(s). > It may contain information that is privileged and confidential. Any > unauthorized review, use, disclosure, or distribution is prohibited. > If you are not the intended recipient, please delete this message, and > reply to the sender regarding the error in a separate email. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. From dont8know8me <@t> yahoo.com Sat Sep 21 06:36:51 2013 From: dont8know8me <@t> yahoo.com (richard peralta) Date: Sat Sep 21 06:37:10 2013 Subject: [Histonet] Salutations Histonet! Message-ID: <20130921113656.OXQL3894.eastrmfepo103.cox.net@eastrmimpo210> Sat, 21 Sep 2013 13:36:51 http://trillionairescoach.billionairescoach.com/wpThumbnails/reddit.php?afbqmcr544ggwshv richard peralta dont8know8me@yahoo.com ~~~~~~~~~~~~~~~~~~~~~~~~~~~~ I heard it was really gnarly: she sucked down a bowl of multi-purpose deodorizing disinfectant and then SMASH! -- Heathers From SteveM <@t> mcclainlab.com Sun Sep 22 09:52:41 2013 From: SteveM <@t> mcclainlab.com (Steve McClain) Date: Sun Sep 22 09:53:07 2013 Subject: [Histonet] Grossing without gloves Message-ID: <012ADA4B5CC00F4AB5E4BAA399E0A5DF5BF106A4@ML1.McClainLabs.local> If you look back 100 years to when gloves were first used in medicine, you will find that gloves were initially used in surgery to protect hands of surgeons and nurse-assistants from caustic effects of carbolic acid and not directly to prevent patient infection. Grossing without gloves is but one of the sins we pathologists were known to have committed in the good old days. Some pathologists formerly grossed without gloves and a few even smoked cigarettes while grossing. ALL 8 community hospital labs where this pathologist worked in the late 1980's and early 1990's had poor ventilation. When it came time to build my own lab, that experience was not forgotten. Steve 631 361 4000 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Saturday, September 21, 2013 1:05 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 118, Issue 39 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: The Good Old Days... (Blazek, Linda) 2. RE: The Good Old Days... (O'Donnell, Bill) 3. Salutations Histonet! (richard peralta) ---------------------------------------------------------------------- Message: 1 Date: Fri, 20 Sep 2013 15:24:00 -0400 From: "Blazek, Linda" Subject: RE: [Histonet] The Good Old Days... To: James Watson , "'Goins, Tresa'" , "Victor A. Tobias" , Cristi Rigazio , "Davis, Cassie" Cc: "histonet@lists.utsouthwestern.edu" Message-ID: <5A2BD13465E061429D6455C8D6B40E39165E403EC3@IBMB7Exchange.digestivespecialists.com> Content-Type: text/plain; charset="iso-8859-1" One has been missed! Doing gross without gloves. There was a pathologist that claimed to be able to feel the lymph nodes much better without gloves. -----Original Message----- From: James Watson [mailto:JWatson@gnf.org] Sent: Friday, September 20, 2013 3:20 PM To: 'Goins, Tresa'; Blazek, Linda; Victor A. Tobias; Cristi Rigazio; Davis, Cassie Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] The Good Old Days... OK 1. Making formalin and Grossing with an old metal table fan to blow the formalin fumes away. 2. Finding the technicon hung up in the morning with the samples dried out. 3. Figuring out how to reprocess the tissue 4. Pan embedding and having the paper labels float away from the samples in the pan. a. Bunsen burner needed 5. Big kitchen knife heated over a Bunsen burner to make blocks 6. Soaking re-useable metal cassettes in a bucket of xylene. (next to where you were pan embedding) 7. Sharpening a knife on multiple stones then stropping on leather then cloth. 8. Over tightening the microtome clamp on the paraffin block and having to re-embed because the block broke. 9. Making staining solutions (some of us still do this). a. Making Schiffs regent without a hood b. Cresyl violet stain powder floating around the room, watch where you put your hands c. Silver nitrate spilled on the counter where you just put your hand then wiped your face. 10. Staining slides in open staining dishes with no fume hood. 11. Wiping the whole room down with xylene to clean the wax up. 12. Autopsies in the afternoon, 13. Whole human brains soaking in the sink in 5 gallons of 20% formalin overnight. Oh boy, really miss them good old days. Life was easier then! Hey Teri, When are we going to get our new coverslipper in? We were spoiled by the demo unit. Jamie James Watson HT? ASCP GNF? Genomics Institute of the Novartis Research Foundation Tel??? 858-332-4647 Fax?? 858-812-1915 jwatson@gnf.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Goins, Tresa Sent: Friday, September 20, 2013 11:33 AM To: Blazek, Linda; Victor A. Tobias; Cristi Rigazio; Davis, Cassie Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] The Good Old Days... I've stropped blades until they looked like scythes - and I got pretty good at adjusting those three screws to line up my block on the microtome. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Friday, September 20, 2013 12:15 PM To: Victor A. Tobias; Cristi Rigazio; Davis, Cassie Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] The Good Old Days... How many can strop a knife? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victor A. Tobias Sent: Friday, September 20, 2013 2:01 PM To: Cristi Rigazio; Davis, Cassie Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] The Good Old Days... Seems like no one has touched upon the fine art of knife sharpening. Sometimes I felt like an executioner sharpening my axe to the point of splitting a hair. Heaven forbid if you hit a staple. That part of the blade was no useless for days to weeks until the daily sharpening ground it out. Life is pretty good when you just pull out another blade from the plastic box. Victor Victor Tobias HT(ASCP) Clinical Applications Analyst Harborview Medical Center Dept of Pathology Room NJB 244 Ninth & Jefferson Seattle, WA 98104 vtobias@u.washington.edu 206-744-2735 206-744-8240 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cristi Rigazio Sent: Friday, September 20, 2013 10:40 AM To: Davis, Cassie Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] The Good Old Days... Hear hear! I agree and was just saying I love hearing the stories (although I am not young). Thanks all for sharing these memories and lessons! Sent from my iPhone On Sep 20, 2013, at 10:13 AM, "Davis, Cassie" wrote: > I enjoy hearing sincere reminiscing...Even though us "kids" don't know how good we have it, some of us enjoy having an "old tech" beside us on the bench. I find weeding through the sarcasm can be profitable and in doing so have learned so much. What the old techs did on a daily basis, we only did in the practice lab and when the automated instruments and pre-made solutions that we have come to rely on fail, experience is so very valuable. Only by their blood, sweat and tears have we benefitted however, we have so far to go, let's do it together. > > Cassandra Davis > CDavis@che-east.org > 302-575-8095 > > > > > > Confidentiality Notice: > This e-mail, including any attachments is the property of Catholic > Health East and is intended for the sole use of the intended > recipient(s). > It may contain information that is privileged and confidential. Any > unauthorized review, use, disclosure, or distribution is prohibited. > If you are not the intended recipient, please delete this message, and > reply to the sender regarding the error in a separate email. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Fri, 20 Sep 2013 19:26:31 +0000 From: "O'Donnell, Bill" Subject: RE: [Histonet] The Good Old Days... To: "Blazek, Linda" , "Victor A. Tobias" , Cristi Rigazio , "Davis, Cassie" Cc: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" I can! I can! And still strop my straight razor every day as well as all the grossing knives and my kitchen knives as often as needed. (Different strops) - Bill -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Friday, September 20, 2013 1:15 PM To: Victor A. Tobias; Cristi Rigazio; Davis, Cassie Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] The Good Old Days... How many can strop a knife? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victor A. Tobias Sent: Friday, September 20, 2013 2:01 PM To: Cristi Rigazio; Davis, Cassie Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] The Good Old Days... Seems like no one has touched upon the fine art of knife sharpening. Sometimes I felt like an executioner sharpening my axe to the point of splitting a hair. Heaven forbid if you hit a staple. That part of the blade was no useless for days to weeks until the daily sharpening ground it out. Life is pretty good when you just pull out another blade from the plastic box. Victor Victor Tobias HT(ASCP) Clinical Applications Analyst Harborview Medical Center Dept of Pathology Room NJB 244 Ninth & Jefferson Seattle, WA 98104 vtobias@u.washington.edu 206-744-2735 206-744-8240 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cristi Rigazio Sent: Friday, September 20, 2013 10:40 AM To: Davis, Cassie Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] The Good Old Days... Hear hear! I agree and was just saying I love hearing the stories (although I am not young). Thanks all for sharing these memories and lessons! Sent from my iPhone On Sep 20, 2013, at 10:13 AM, "Davis, Cassie" wrote: > I enjoy hearing sincere reminiscing...Even though us "kids" don't know how good we have it, some of us enjoy having an "old tech" beside us on the bench. I find weeding through the sarcasm can be profitable and in doing so have learned so much. What the old techs did on a daily basis, we only did in the practice lab and when the automated instruments and pre-made solutions that we have come to rely on fail, experience is so very valuable. Only by their blood, sweat and tears have we benefitted however, we have so far to go, let's do it together. > > Cassandra Davis > CDavis@che-east.org > 302-575-8095 > > > > > > Confidentiality Notice: > This e-mail, including any attachments is the property of Catholic > Health East and is intended for the sole use of the intended > recipient(s). > It may contain information that is privileged and confidential. Any > unauthorized review, use, disclosure, or distribution is prohibited. > If you are not the intended recipient, please delete this message, and > reply to the sender regarding the error in a separate email. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. ------------------------------ Message: 3 Date: Sat, 21 Sep 2013 13:36:51 +0200 From: "richard peralta" Subject: [Histonet] Salutations Histonet! To: "Histonet" Message-ID: <20130921113656.OXQL3894.eastrmfepo103.cox.net@eastrmimpo210> Content-Type: text/plain; charset="us-ascii" Sat, 21 Sep 2013 13:36:51 http://trillionairescoach.billionairescoach.com/wpThumbnails/reddit.php?afbqmcr544ggwshv richard peralta dont8know8me@yahoo.com ~~~~~~~~~~~~~~~~~~~~~~~~~~~~ I heard it was really gnarly: she sucked down a bowl of multi-purpose deodorizing disinfectant and then SMASH! -- Heathers ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 118, Issue 39 ***************************************** From rsrichmond <@t> gmail.com Sun Sep 22 17:16:03 2013 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Sun Sep 22 17:16:07 2013 Subject: [Histonet] Re: Grossing without gloves Message-ID: Steve McClain describes grossing without gloves. When I was a pathology resident around 1970, we usually wore gloves to handle gross specimens, always if the specimen was in formalin. We always did frozen sections without gloves. The cryostat had just been introduced, and many of the old-timers continued to use the old wet knife method. I confess I still do frozens without gloves if nobody's watching. I also should probably mention that I've worked with three pathologists of about my generation who caught tuberculosis in line of duty, one of whom probably died of it. Bob Richmond Samurai Pathologist Maryville TN From Ken_Marissael <@t> vwr.com Mon Sep 23 13:05:30 2013 From: Ken_Marissael <@t> vwr.com (Ken_Marissael@vwr.com) Date: Mon Sep 23 13:05:41 2013 Subject: [Histonet] Ken Marissael is out of the office Message-ID: I will be out of the office starting 09/23/2013 and will not return until 09/25/2013. I will be away working at the NSH Show in Providence. During my absense Kerri O'Brien will be covering my territory. She can be reached via e-mail (kerri_obrien@vwr.com or by cell (551-497-0848) If you have an emergency, please contact - healthcareservice.com or customer service at 877-881-1192. From brannon <@t> alliedsearchpartners.com Mon Sep 23 13:43:09 2013 From: brannon <@t> alliedsearchpartners.com (Brannon Owens) Date: Mon Sep 23 13:43:21 2013 Subject: [Histonet] Histology Supervisor- southwest FL Message-ID: Needing a Histology Supervisor for a southwest FL area client. Five years of experience (including supervisory experience) in routine histology including special stains and IHC. Send an updated resume to brannon@alliedsearchpartners.com to be considered. A full job description is on the Allied Search Partners website. Brannon Owens Recruitment Manager Allied Search Partners LinkedIn: http://www.linkedin.com/pub/brannon-owens/28/528/823 http://www.alliedsearchpartners.com T: 888.388.7571 ext. 106 F: 888.388.7572 From mbplab <@t> yahoo.com Mon Sep 23 16:00:57 2013 From: mbplab <@t> yahoo.com (Mary Benoit) Date: Mon Sep 23 16:01:03 2013 Subject: [Histonet] edge effect on IHC Message-ID: <1379970057.43816.YahooMailNeo@web142505.mail.bf1.yahoo.com> I have been aware of some "edge effect" on an occasional slide, but lately we noticed the effect is showing up in the area of the slide that is farthest from the labels. We use DAKO Link Autostainer and their pretreatment baths for everything. One case in particular we stained a large melanoma met to colon that was well fixed and processed that stained only the edge, however when we sent block to Neogenomics reference lab, their slide stained evenly over the entire tumor area. We both used a melanoma cocktail. Not sure what is going on. Any suggestions? Thank you Mary F Benoit, MT(ASCP) The Pathology Laboratory 830 Bayou Pines Drive Lake Charles, LA 70601 mbplab@yahoo.com From ruio7 <@t> hotmail.com Mon Sep 23 21:19:01 2013 From: ruio7 <@t> hotmail.com (Rui TAHARA) Date: Mon Sep 23 21:19:08 2013 Subject: [Histonet] Undecalcified sample in paraffin and plastic media Message-ID: I have undecalcified paraffin embed samples that were sectioned at 10 micron that I want to stain with Von kossa. Because samples are embryonic quail heads (ossification starts to happen) and still soft enough to section with standard rotary microtome with tungsten knife in paraffin. My intention is to 3D reconstruct anatomies based on histological sections. Because of this, I am wondering if I should actually use plastic media rather than paraffin to keep the section shape as consistent as possible. Does plastic embed material actually preserve the consistent shape among sections better than paraffin embed sample? No winkle etc..? Is there any other advantage that I actually should use the plastic media than paraffin for what I want to do? I know downside of plastic media is that in general plastic embedding process are lengthy and plastic embedding material are expensive than the paraffin ones, and are mainly use for bone to support the hard material for sectioning. When I sectioned some ossified samples, beak start to fall off from section and the section show the lines from the possibly scratched knife. Is this indication of paraffin media that does not provide enough strength for sectioning? I thought it may possibly the poor infiltration. In our lab nobody has processed the plastic embedding and sectioning (we have only standard microtome, no vaccum machine. Can I section plastic embed sample with the standard microtome at 10 micron?) so I would like to have any input before actually making a plastic embed sample. Any suggestions would be appreciated. Rui TAHARA Biology Department McGill University From sccrshlly <@t> yahoo.com Tue Sep 24 07:16:08 2013 From: sccrshlly <@t> yahoo.com (Shelly Coker) Date: Tue Sep 24 07:16:13 2013 Subject: [Histonet] (no subject) Message-ID: <20130924121605.FTGY3897.eastrmfepo102.cox.net@eastrmimpo209> Tue, 24 Sep 2013 07:16:08 http://mcleodsculpture.com/butcher/bbc.php?zxxyuddb975xyr Shelly Coker sccrshlly@yahoo.com ******************** YOU KLINGON BASTARD! YOU ATE MY LUNCH!! -- Vince Farquharson From mw <@t> personifysearch.com Tue Sep 24 07:23:06 2013 From: mw <@t> personifysearch.com (Matt Ward) Date: Tue Sep 24 07:23:16 2013 Subject: [Histonet] New Histology Opportunities Message-ID: <01ba01ceb920$d2eafa70$78c0ef50$@personifysearch.com> Good morning Histonet! We have recently had a few new opportunities open with a world leading manufacturer of Histology products. Please contact me directly at mw@personifysearch.com or at 800.875.6188 ext. 103 to learn more. R&D Histotech - Richmond, IL Field Histology Support Specialist- IHC - Northern NJ/NYC Field Histology Support Specialist - California Have a wonderful day! Matt Ward Account Executive Personify 5020 Weston Parkway Suite 315 Cary NC 27513 (Tel) 800.875.6188 direct ext 103 (Fax) 919.460.0642 www.personifysearch.com From SHUNTER <@t> beaumont.edu Tue Sep 24 07:23:53 2013 From: SHUNTER <@t> beaumont.edu (Sue Hunter) Date: Tue Sep 24 07:23:59 2013 Subject: [Histonet] RE: Venata Her2 on Ultra In-Reply-To: <5F46C87AD649864F9FA8273E8E31507A02739CA7@SWM2006.lhsnt.legacyhs> References: <5F46C87AD649864F9FA8273E8E31507A02739CA7@SWM2006.lhsnt.legacyhs> Message-ID: We have also tried to optimize the HER2 on the Ultra - our pathologists is not happy with how strong it is. He is very concerned with over calling the positives and causing unnecessary treatments. We currently use the Herceptest from Dako on a Lab Vision stainer but may have to eventually move to the Ultra. We FISH all of our cases, but if the IHC is reported out as positive, then the clinician will put the patient on therapy which has serious side effects and is very costly. If it is not truly a positive tumor, the patient should not be receiving the therapy. A very difficult problem for us. Sue Sue Hunter, Supervisor Advanced Diagnostics Beaumont Health System Royal Oak MI 248-898-5146 shunter@beaumont.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Wellen, Terry D :GS Histology Sent: Thursday, September 19, 2013 1:36 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Venata Her2 on Ultra Is anybody doing Her2 on the Ventana Ultra Platform? I am having trouble optimizing to my Pathologists satisfaction. Terrence D. Wellen HT(ASCP) Technical Specialist Legacy Good Samaritan Hospital Portland, OR 503-413-8954 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SHUNTER <@t> beaumont.edu Tue Sep 24 07:29:33 2013 From: SHUNTER <@t> beaumont.edu (Sue Hunter) Date: Tue Sep 24 07:29:39 2013 Subject: [Histonet] Peggy Wenks award Message-ID: For those of you who may not be attending the National Convention or did not hear, we at Beaumont are so proud to announce that Peggy "Amazing" Wenk has won the J.B. McCormick Award. This very prestigious award was presented by Dr. McCormick and was given to Peggy for her outstanding and exceptional service to the NSH. Please join us in congratulating Peggy on this well deserved recognition and award. Sue Sue Hunter, Supervisor Advanced Diagnostics Beaumont Health System Royal Oak MI 248-898-5146 shunter@beaumont.edu From Joyce.Weems <@t> emoryhealthcare.org Tue Sep 24 07:57:27 2013 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Tue Sep 24 07:57:48 2013 Subject: [Histonet] RE: Peggy Wenks award In-Reply-To: References: Message-ID: Congrats, Peggy. Very well deserved!! Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sue Hunter Sent: Tuesday, September 24, 2013 8:30 AM To: 'Histonet (histonet@lists.utsouthwestern.edu)'; histonet-bounces@lists.utsouthwestern.edu Subject: [Histonet] Peggy Wenks award For those of you who may not be attending the National Convention or did not hear, we at Beaumont are so proud to announce that Peggy "Amazing" Wenk has won the J.B. McCormick Award. This very prestigious award was presented by Dr. McCormick and was given to Peggy for her outstanding and exceptional service to the NSH. Please join us in congratulating Peggy on this well deserved recognition and award. Sue Sue Hunter, Supervisor Advanced Diagnostics Beaumont Health System Royal Oak MI 248-898-5146 shunter@beaumont.edu ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From akemiat3377 <@t> yahoo.com Tue Sep 24 09:07:18 2013 From: akemiat3377 <@t> yahoo.com (Akemi Allison) Date: Tue Sep 24 09:07:23 2013 Subject: [Histonet] Jules Elias IHC Award Message-ID: <1380031638.48208.YahooMailNeo@web140605.mail.bf1.yahoo.com> Congratulations?Jim Burchette?on receiving the Jules Elias IHC Award. Well deserved! ?Just emailed Jules to let him know. ? Akemi Allison BS, HT(ASCP)HTL Director Phoenix Lab Consulting E-Mail: akemiat3377@yahoo.com From anolan <@t> prometheushealthcare.com Tue Sep 24 09:29:40 2013 From: anolan <@t> prometheushealthcare.com (anolan@prometheushealthcare.com) Date: Tue Sep 24 09:29:32 2013 Subject: [Histonet] Histology Supervisor Opportunity- Tallahassee, FL Message-ID: <003801ceb932$82f0b700$88d22500$@prometheushealthcare.com> Hi All, I'm currently recruiting for a histology supervisor for a private lab in Tallahassee, FL. A minimum of 5 years previous supervisory experience is required. The ideal candidate will possess excellent leadership, interpersonal, and customer service skills. Must be HT or HTL(ASCP). Florida license (or eligibility) is required. If you'd like to apply please follow up with me directly via email or telephone. Anna Nolan Recruiter Prometheus Healthcare Direct Line 301-693-8908 Fax 301-368-2478 anolan @prometheushealthcare.com http://www.linkedin.com/pub/annelise-nolan/55/ba0/ab6 www.prometheushealthcare.com From rjbuesa <@t> yahoo.com Tue Sep 24 09:35:54 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Sep 24 09:35:59 2013 Subject: [Histonet] edge effect on IHC In-Reply-To: <1379970057.43816.YahooMailNeo@web142505.mail.bf1.yahoo.com> References: <1379970057.43816.YahooMailNeo@web142505.mail.bf1.yahoo.com> Message-ID: <1380033354.24204.YahooMailNeo@web163103.mail.bf1.yahoo.com> Sometimes that "edge effect" is caused by drying when there is not enough reagents. If you are using an automated stainer, please check the amount of delivered reagents. Ren? J. ________________________________ From: Mary Benoit To: "histonet@lists.utsouthwestern.edu" Sent: Monday, September 23, 2013 5:00 PM Subject: [Histonet] edge effect on IHC I have been aware of some "edge effect" on an occasional slide, but lately we noticed the effect is showing up in the area of the slide that is farthest from the labels. We use DAKO Link Autostainer and their pretreatment baths for everything. One case in particular we stained a large melanoma met to colon that was well fixed and processed that stained only the edge, however when we sent block to Neogenomics reference lab, their slide stained evenly over the entire tumor area. We both used a melanoma cocktail. Not sure what is going on. Any suggestions? Thank you Mary F Benoit, MT(ASCP) The Pathology Laboratory 830 Bayou Pines Drive Lake Charles, LA 70601 mbplab@yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From philip_manfre <@t> merck.com Tue Sep 24 09:51:23 2013 From: philip_manfre <@t> merck.com (Manfre, Philip) Date: Tue Sep 24 09:51:28 2013 Subject: [Histonet] edge effect on IHC In-Reply-To: <1380033354.24204.YahooMailNeo@web163103.mail.bf1.yahoo.com> References: <1379970057.43816.YahooMailNeo@web142505.mail.bf1.yahoo.com> <1380033354.24204.YahooMailNeo@web163103.mail.bf1.yahoo.com> Message-ID: <558A4571351D0C42BD923F403F4198C4B270B11270@USCTMXP51014.merck.com> I concur with Rene'. That was my first thought. Edges drying up on an autostainer due to hydrophobia or insufficient amounts of solution. Stainers usually have the option to adjust the volume that is applied and sometimes even the location on the slide (zones). Thorough deparaffinization is also essential to avoid the hydrophobic repelling of solutions and wash buffers. Also be sure your reagents have some form of surfactant or detergent (e.g.Tween 20) in them to break the surface tension and allow the even spread of whatever is being applied. Phil. Philip Manfre, B.A., HT (ASCP) Associate Principal Scientist Merck Research Laboratories WP45-251 PO Box 4 West Point, PA 19486 215-652-9750 215-993-0383 (fax) philip_manfre@merck.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, September 24, 2013 10:36 AM To: Mary Benoit; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] edge effect on IHC Sometimes that "edge effect" is caused by drying when there is not enough reagents. If you are using an automated stainer, please check the amount of delivered reagents. Ren? J. ________________________________ From: Mary Benoit To: "histonet@lists.utsouthwestern.edu" Sent: Monday, September 23, 2013 5:00 PM Subject: [Histonet] edge effect on IHC I have been aware of some "edge effect" on an occasional slide, but lately we noticed the effect is showing up in the area of the slide that is farthest from the labels. We use DAKO Link Autostainer and their pretreatment baths for everything. One case in particular we stained a large melanoma met to colon that was well fixed and processed that stained only the edge, however when we sent block to Neogenomics reference lab, their slide stained evenly over the entire tumor area. We both used a melanoma cocktail. Not sure what is going on. Any suggestions? Thank you Mary F Benoit, MT(ASCP) The Pathology Laboratory 830 Bayou Pines Drive Lake Charles, LA 70601 mbplab@yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From cmiller <@t> gladstone.ucsf.edu Tue Sep 24 10:13:10 2013 From: cmiller <@t> gladstone.ucsf.edu (Caroline Miller) Date: Tue Sep 24 10:13:19 2013 Subject: [Histonet] edge effect on IHC In-Reply-To: <558A4571351D0C42BD923F403F4198C4B270B11270@USCTMXP51014.merck.com> References: <1379970057.43816.YahooMailNeo@web142505.mail.bf1.yahoo.com> <1380033354.24204.YahooMailNeo@web163103.mail.bf1.yahoo.com> <558A4571351D0C42BD923F403F4198C4B270B11270@USCTMXP51014.merck.com> Message-ID: <78C1201F-E54D-4670-9249-AB31CAACF761@gladstone.ucsf.edu> However, I use the Shandon sequenza, and I still see edge effect. I always thought it was a fixation related. Maybe even that the edge staining is 'more real' than the rest!! Histology, it's all voodoo!! Caroline Caroline Miller Gladstone Institutes www.gladstoneinstitutes.org Tel: 415 7342566 Cell: 415 2187297 On Sep 24, 2013, at 7:51 AM, "Manfre, Philip" wrote: > I concur with Rene'. That was my first thought. Edges drying up on an autostainer due to hydrophobia or insufficient amounts of solution. Stainers usually have the option to adjust the volume that is applied and sometimes even the location on the slide (zones). Thorough deparaffinization is also essential to avoid the hydrophobic repelling of solutions and wash buffers. Also be sure your reagents have some form of surfactant or detergent (e.g.Tween 20) in them to break the surface tension and allow the even spread of whatever is being applied. > > Phil. > > > Philip Manfre, B.A., HT (ASCP) > Associate Principal Scientist > Merck Research Laboratories > WP45-251 > PO Box 4 > West Point, PA 19486 > > 215-652-9750 > 215-993-0383 (fax) > philip_manfre@merck.com > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa > Sent: Tuesday, September 24, 2013 10:36 AM > To: Mary Benoit; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] edge effect on IHC > > Sometimes that "edge effect" is caused by drying when there is not enough reagents. If you are using an automated stainer, please check the amount of delivered reagents. > Ren? J. > > > ________________________________ > From: Mary Benoit > To: "histonet@lists.utsouthwestern.edu" > Sent: Monday, September 23, 2013 5:00 PM > Subject: [Histonet] edge effect on IHC > > > I have been aware of some "edge effect" on an occasional slide, but lately we noticed the effect is showing up in the area of the slide that is farthest from the labels. We use DAKO Link Autostainer and their pretreatment baths for everything. One case in particular we stained a large melanoma met to colon that was well fixed and processed that stained only the edge, however when we sent block to Neogenomics reference lab, their slide stained evenly over the entire tumor area. We both used a melanoma cocktail. Not sure what is going on. Any suggestions? > Thank you > Mary F Benoit, MT(ASCP) > The Pathology Laboratory > 830 Bayou Pines Drive > Lake Charles, LA 70601 > mbplab@yahoo.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Notice: This e-mail message, together with any attachments, contains > information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, > New Jersey, USA 08889), and/or its affiliates Direct contact information > for affiliates is available at > http://www.merck.com/contact/contacts.html) that may be confidential, > proprietary copyrighted and/or legally privileged. It is intended solely > for the use of the individual or entity named on this message. If you are > not the intended recipient, and have received this message in error, > please notify us immediately by reply e-mail and then delete it from > your system. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 <@t> earthlink.net Tue Sep 24 10:31:59 2013 From: relia1 <@t> earthlink.net (Pam Barker) Date: Tue Sep 24 10:31:59 2013 Subject: [Histonet] RELIA Hot Histology Job Opportunity Update 09/24/2013 It's a Beautiful Fall Day in Providence, Rhode Island at the 40th Annual NSH Symposium and Convention Message-ID: <032301ceb93b$35db0c00$a1912400$@earthlink.net> Hi Histonetters!! I hope everyone is having a great day. Especially those of you who have the opportunity to be attending the NSH/SC currently being held in beautiful Providence Rhode Island. From what I understand it is a typical sunny crisp cool day and the ticket auction is in full swing in the exhibit hall as the morning sessions are coming to a close. For those of us here at home I have a few opportunities I would like to tell you about. These are full time permanent positions. All of my clients offer excellent compensation, benefits and in most cases relocation assistance And without further ado . Here they are: Histology Supervisor -Tallahasse, FL Histology Supervisor - Boston, MA Lead Histology Tech - Boston, MA Lead Histology Tech - Columbus, OH Histotechnician - Harrisonburg, VA Histotechnician - Columbus, OH Histotechnician - Nashville, TN Histotechnician - Tyler, TX Histotechnician - Austin, TX Histotech - Augusta, GA Histotech - Atlanta, GA If you or anyone you know is interested in any of these positions or would like to engage in a customized job search please contact me. I would be happy to help you and if we both help your friend or coworker you will earn a referral fee. Have A Great Day!! Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From Timothy.Morken <@t> ucsfmedctr.org Tue Sep 24 10:37:23 2013 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Tue Sep 24 10:37:41 2013 Subject: [Histonet] Labels for Bond Stainers using LIS interface Message-ID: <761E2B5697F795489C8710BCC72141FF09AEEE@ex07.net.ucsf.edu> Histonetters, For those labs using a direct LIS link to Leica Bond stainers, and are using a label printed from the LIS to ID slides for the Bond: Have you been able to validate the use of labels larger than the 15x22 that Leica uses for the Bond system? Would you please tell me what size label, and what label vendors and product numbers you use. We are planning to use Zebra thermal printers. We have heard that up to 22 x 22 will work, but I don't have specific references or suppliers for those labels. Thanks for ANY info! Tim Morken Lead, Pathology Barcoding Department of Pathology UC San Francisco Medical Center 505 Parnassus Ave, Box 1656 Room S570 San Francisco, CA 94143 (415) 353-1266 (ph) (415) 514-3403 (fax) tim.morken@ucsfmedctr.org From rjbuesa <@t> yahoo.com Tue Sep 24 10:43:25 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Sep 24 10:43:29 2013 Subject: [Histonet] edge effect on IHC In-Reply-To: <78C1201F-E54D-4670-9249-AB31CAACF761@gladstone.ucsf.edu> References: <1379970057.43816.YahooMailNeo@web142505.mail.bf1.yahoo.com> <1380033354.24204.YahooMailNeo@web163103.mail.bf1.yahoo.com> <558A4571351D0C42BD923F403F4198C4B270B11270@USCTMXP51014.merck.com> <78C1201F-E54D-4670-9249-AB31CAACF761@gladstone.ucsf.edu> Message-ID: <1380037405.10236.YahooMailNeo@web163104.mail.bf1.yahoo.com> That artifact cannot be fixation related because if there is some part of the sample that is well fixed is the outer limit, where the NBF enters. It does not matter what stainer you use, they all?can deliver incorrect amounts of reagents. Ren? J.? ? ________________________________ From: Caroline Miller To: "Manfre, Philip" Cc: Rene J Buesa ; Mary Benoit ; "histonet@lists.utsouthwestern.edu" Sent: Tuesday, September 24, 2013 11:13 AM Subject: Re: [Histonet] edge effect on IHC However, I use the Shandon sequenza, and I still see edge effect. I always thought it was a fixation related. Maybe even that the edge staining is 'more real' than the rest!! Histology, it's all voodoo!! Caroline Caroline Miller Gladstone Institutes www.gladstoneinstitutes.org Tel: 415 7342566 Cell: 415 2187297 On Sep 24, 2013, at 7:51 AM, "Manfre, Philip" wrote: > I concur with Rene'.? That was my first thought. Edges drying up on an autostainer due to hydrophobia or insufficient amounts of solution. Stainers usually have the option to adjust the volume that is applied and sometimes even the location on the slide (zones).? Thorough deparaffinization is also essential to avoid the hydrophobic repelling of solutions and wash buffers.? Also be sure your reagents have some form of surfactant or detergent (e.g.Tween 20) in them to break the surface tension and allow the even spread of whatever is being applied. > > Phil. > > > Philip Manfre, B.A., HT (ASCP) > Associate Principal Scientist > Merck Research Laboratories > WP45-251 > PO Box 4 > West Point, PA 19486 > > 215-652-9750 > 215-993-0383 (fax) > philip_manfre@merck.com > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa > Sent: Tuesday, September 24, 2013 10:36 AM > To: Mary Benoit; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] edge effect on IHC > > Sometimes that "edge effect" is caused by drying when there is not enough reagents. If you are using an automated stainer, please check the amount of delivered reagents. > Ren? J. > > > ________________________________ > From: Mary Benoit > To: "histonet@lists.utsouthwestern.edu" > Sent: Monday, September 23, 2013 5:00 PM > Subject: [Histonet] edge effect on IHC > > > I have been aware of some "edge effect" on an occasional slide, but lately we noticed the effect is showing up in the area of the slide that is farthest from the labels. We use DAKO Link Autostainer and their pretreatment baths for everything. One case in particular we stained a large melanoma met to colon that was well fixed and processed that stained only the edge, however when we sent block to Neogenomics reference lab, their slide stained evenly over the entire tumor area. We both used a melanoma cocktail. Not sure what is going on. Any suggestions? > Thank you > Mary F Benoit, MT(ASCP) > The Pathology Laboratory > 830 Bayou Pines Drive > Lake Charles, LA 70601 > mbplab@yahoo.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Notice:? This e-mail message, together with any attachments, contains > information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, > New Jersey, USA 08889), and/or its affiliates Direct contact information > for affiliates is available at > http://www.merck.com/contact/contacts.html) that may be confidential, > proprietary copyrighted and/or legally privileged. It is intended solely > for the use of the individual or entity named on this message. If you are > not the intended recipient, and have received this message in error, > please notify us immediately by reply e-mail and then delete it from > your system. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jclark <@t> pcnm.com Tue Sep 24 12:53:56 2013 From: jclark <@t> pcnm.com (Joanne Clark) Date: Tue Sep 24 12:54:07 2013 Subject: [Histonet] Submitting Questions In-Reply-To: <20130924170732.4B94DA7DF06@mx10.myoutlookonline.com> References: <20130924170732.4B94DA7DF06@mx10.myoutlookonline.com> Message-ID: <0494A7D4E8CC254EA2FB81464982E378B4ABA154@S10MAILD001N3.SH10.lan> Can anyone out in histoland explain to me why my submitted questions are not making it through to histonet? I submitted a question on the 19th of September, and I still have not seen it appear in the group emails I get daily. I sent the question to the correct address (I checked it to be sure). When I respond to someones question, I can see my responses, I just can't seem to get an original question submitted. Can anyone tell me what I am doing wrong? Disclaimer: This electronic message may contain information that is proprietary, confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From CThornton <@t> dahlchase.com Tue Sep 24 13:40:15 2013 From: CThornton <@t> dahlchase.com (Clare Thornton) Date: Tue Sep 24 13:40:21 2013 Subject: [Histonet] ER/PR results tracking Message-ID: How is everyone tracking their ER/PR results to comply with the following: ANP.22970 Annual Result Comparison Phase I For immunohistochemical and FISH/ISH tests that provide independent predictive information, the laboratory at least annually compares its patient results with published benchmarks, and evaluates interobserver variability among the pathologists in the laboratory. NOTE: Individuals interpreting the assay must also have their concordance compared with each other and this concordance should also be at least 95%. With specific reference to estrogen and progesterone receptor studies: in general, the overall proportion of ER-negative breast cancers (invasive and DCIS) should not exceed 30%. The average is somewhat lower in postmenopausal than premenopausal women (approximately 20% vs. 35%). The average is considerably lower in well-differentiated carcinomas (<10%) and certain special types of invasive carcinomas (<10% in lobular, tubular, and mucinous types). The proportion of PgR-negative cases is 10-15% higher than for ER in each of these settings. Investigation is warranted if the proportion of negative cases is significantly higher in any of these settings. Our LIS doesn't have an easy way of compiling these results. Basically, we are stuck with searching all cases with ER/PR done and manually reading and recording results. Does anyone have a good shortcut or idea of how to compile these results when your LIS doesn't keep track of the results for you? Thanks in advance! Clare J. Thornton, HTL(ASCP)QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com From flnails <@t> texaschildrens.org Tue Sep 24 13:57:44 2013 From: flnails <@t> texaschildrens.org (Nails, Felton) Date: Tue Sep 24 13:57:53 2013 Subject: [Histonet] RE: Submitting Questions In-Reply-To: <0494A7D4E8CC254EA2FB81464982E378B4ABA154@S10MAILD001N3.SH10.lan> References: <20130924170732.4B94DA7DF06@mx10.myoutlookonline.com> <0494A7D4E8CC254EA2FB81464982E378B4ABA154@S10MAILD001N3.SH10.lan> Message-ID: <327E034F1892504289B7A17EC71DF9F303B9D1@TCFMSG03.ad.texaschildrenshospital.org> I am in the same situation??? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joanne Clark Sent: Tuesday, September 24, 2013 12:54 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Submitting Questions Can anyone out in histoland explain to me why my submitted questions are not making it through to histonet? I submitted a question on the 19th of September, and I still have not seen it appear in the group emails I get daily. I sent the question to the correct address (I checked it to be sure). When I respond to someones question, I can see my responses, I just can't seem to get an original question submitted. Can anyone tell me what I am doing wrong? Disclaimer: This electronic message may contain information that is proprietary, confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ______________________________________________________________________ From Mark.Elliott <@t> hli.ubc.ca Tue Sep 24 14:09:04 2013 From: Mark.Elliott <@t> hli.ubc.ca (Mark Elliott) Date: Tue Sep 24 14:09:11 2013 Subject: [Histonet] Ceramide antibodies Message-ID: <524180E0020000D6000659E5@mail.hli.ubc.ca> I have been asked to do some staining for ceramide in human lungs. Anyone have recommendations for an antibody that works well on human tissues? Any tips would be greatly appreciated. This is for either frozen tissue or FFPE. Thanks Mark From doug.porter <@t> caplab.org Tue Sep 24 14:11:25 2013 From: doug.porter <@t> caplab.org (Douglas Porter) Date: Tue Sep 24 14:09:26 2013 Subject: [Histonet] RE: Submitting Questions In-Reply-To: <327E034F1892504289B7A17EC71DF9F303B9D1@TCFMSG03.ad.texaschildrenshospital.org> References: <20130924170732.4B94DA7DF06@mx10.myoutlookonline.com> <0494A7D4E8CC254EA2FB81464982E378B4ABA154@S10MAILD001N3.SH10.lan> <327E034F1892504289B7A17EC71DF9F303B9D1@TCFMSG03.ad.texaschildrenshospital.org> Message-ID: <003701ceb959$ddea70c0$99bf5240$@caplab.org> Check to make sure your Histonet email address is correct. When you reply to one the email is already entered. That may be why you can see your responses and not your original messages. Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.porter@caplab.org www.caplab.org ? ? The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, copying, forwarding or capture of this communication is strictly prohibited. If you have received this communication in error, please notify me immediately by return e-mail and delete this and all copies. Thank-you. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nails, Felton Sent: Tuesday, September 24, 2013 2:58 PM To: 'Joanne Clark'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Submitting Questions I am in the same situation??? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joanne Clark Sent: Tuesday, September 24, 2013 12:54 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Submitting Questions Can anyone out in histoland explain to me why my submitted questions are not making it through to histonet? I submitted a question on the 19th of September, and I still have not seen it appear in the group emails I get daily. I sent the question to the correct address (I checked it to be sure). When I respond to someones question, I can see my responses, I just can't seem to get an original question submitted. Can anyone tell me what I am doing wrong? Disclaimer: This electronic message may contain information that is proprietary, confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jorourke <@t> allied360.com Tue Sep 24 14:15:23 2013 From: jorourke <@t> allied360.com (Judy O'Rourke) Date: Tue Sep 24 14:15:47 2013 Subject: [Histonet] Article: Anatomic Pathology Takes a Leap in Quality Process Improvement Message-ID: William DeSalvo, BS HTL(ASCP), has written the cover article in CLP's September issue: Anatomic Pathology Takes a Leap in Quality Process Improvement Found here in the digital edition (page 8): http://edition.pagesuite-professional.co.uk//launch.aspx?pbid=db6b2441-3876-40c8-8eb3-8a480a616c4e Regards, Judy O'Rourke Editor/CLP From Mark.Elliott <@t> hli.ubc.ca Tue Sep 24 14:40:58 2013 From: Mark.Elliott <@t> hli.ubc.ca (Mark Elliott) Date: Tue Sep 24 14:41:01 2013 Subject: [Histonet] Re: Histonet Digest, Vol 118, Issue 42 In-Reply-To: References: Message-ID: <5241885A020000D600065A10@mail.hli.ubc.ca> Well done Peggy!!! Mark From mward <@t> wakehealth.edu Tue Sep 24 14:54:09 2013 From: mward <@t> wakehealth.edu (Martha Ward-Pathology) Date: Tue Sep 24 14:54:16 2013 Subject: [Histonet] RE: Submitting Questions In-Reply-To: <327E034F1892504289B7A17EC71DF9F303B9D1@TCFMSG03.ad.texaschildrenshospital.org> References: <20130924170732.4B94DA7DF06@mx10.myoutlookonline.com> <0494A7D4E8CC254EA2FB81464982E378B4ABA154@S10MAILD001N3.SH10.lan> <327E034F1892504289B7A17EC71DF9F303B9D1@TCFMSG03.ad.texaschildrenshospital.org> Message-ID: I have had the same problem over the past few months... ? Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard ?\? Winston-Salem, NC 27157 p 336.716.2109 ?\? f 336.716.5890 ? mward@wakehealth.edu ? ? ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nails, Felton Sent: Tuesday, September 24, 2013 2:58 PM To: 'Joanne Clark'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Submitting Questions I am in the same situation??? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joanne Clark Sent: Tuesday, September 24, 2013 12:54 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Submitting Questions Can anyone out in histoland explain to me why my submitted questions are not making it through to histonet? I submitted a question on the 19th of September, and I still have not seen it appear in the group emails I get daily. I sent the question to the correct address (I checked it to be sure). When I respond to someones question, I can see my responses, I just can't seem to get an original question submitted. Can anyone tell me what I am doing wrong? Disclaimer: This electronic message may contain information that is proprietary, confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Tue Sep 24 15:44:18 2013 From: pruegg <@t> ihctech.net (pruegg@ihctech.net) Date: Tue Sep 24 15:44:23 2013 Subject: [Histonet] RE: Submitting Questions Message-ID: <20130924134418.f86bd30e73b823f57b516b5451216a98.3f1c6353d6.wbe@email01.secureserver.net> do u see this question posted? -------- Original Message --------< Submitting Questions From: Martha Ward- Date: Tue, September 24, 2013 12:54 pm To: "Nails, Felt Clark' <[3] <[5]histonet@lists.utsouthwestern.edu> I have had the same problem over the past few months... &n Martha Ward, MT (ASCP) QIHC Manager Molecular Medical Center Boulevard \ Winston-Salem p 336.716.2109 \ f 336.716.5890 < -----Original Message---- From: [6] [[7]mailto:histonet-bounces@lists.utsouthweste Nails, Felton Sent: Tuesday, September 24, 2 To: 'Joanne Clark'; [8]histonet@lists.utsouthwestern.edu Subject: [Histo I am in the same situation??? -----Original Message----- From: [9]histonet-bounces@lists.utsouthwestern. o:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joanne Clark< To: [11]histonet@lists.utsouthwestern.edu< [Histonet] Submitting Questions Can anyone out questions are not making it thr question on the 19th of September, and I s appear in the group emails I get daily. I sent the q correct address (I checked it to be sure). When I respond t someones question, I can see my responses, I just can't seem to get an or wrong? Disclaimer: This electronic message may cont is proprietary, confidential, or legally privileged or is intended only for the use of the individual(s) and entity the message. If you are not an intended recipient of this message please notify the sender immediately and delete the material from your co disclose information it contains _______________________________________________ Histon [12] [13]http://lists.utsouthwestern.edu/mail ____________________________________ __________________________________ CONFIDENTIALITY NOTICE: The privileged. If you representative of the int that any review, dissemination, o attachments, if any, or the information prohibited. If you have received this e-mail in error immediately notify the sender by return e-mail and delete this e- mail from your computer system. Thank you. ________________________ ______________________________________________ _______________ Histonet mailing list [14]Histonet@lists.utsouthwestern [15]http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Hist [16]Histonet@lists.utsouthwestern.edu [17]http://lists.utsouthwestern.edu/ma References 1. 3D"mailto:mward@wakehealth.edu" 2. 3D"mailto:flnails@texaschildrens.org" 3. 3D"mailto:jclark@pcnm.com" 4. 3D"mailto:histonet@lists.utsouthwestern.e 5. 3D"mailto:hist 6. 3D"mailto:histonet-bounces@lists.utsouthwestern.edu" 7. 3D"mailto:histonet-b 8. 3D"mailto:histonet@lists.utso 9. 3D"mailto:histon 10. 3D"mailto:histonet-bounces@lists.utsouthwestern.edu" 11. file://localhost/tmp/3D"mailt 12. 3D"mailto:Histonet@lists.utsouthwestern.edu" 13. 3D"http://lists.utsouth=/ 14. ="mailto:Histonet@lists.utsouthwestern.edu" 15. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/ 16. 3D"mailto:Histonet@lists.utsouthwestern.edu 17. 3D"http://lists.utsou=/ From dmccaig <@t> ckha.on.ca Tue Sep 24 15:55:40 2013 From: dmccaig <@t> ckha.on.ca (Diana McCaig) Date: Tue Sep 24 15:55:47 2013 Subject: [Histonet] PowerPath QA Message-ID: Is anyone using this module and are you willing to share any documents regarding it. Or opinions on what think of it for a QA program Sincere thanks Diana From vtobias <@t> uw.edu Tue Sep 24 15:56:26 2013 From: vtobias <@t> uw.edu (Victor A. Tobias) Date: Tue Sep 24 16:00:36 2013 Subject: [Histonet] Ventana Vantage and PowerPath Message-ID: Is anyone out there using this combination of software? If so, please contact me directly. Thanks Victor Victor Tobias HT(ASCP) Clinical Applications Analyst Harborview Medical Center Dept of Pathology Room NJB 244 Ninth & Jefferson Seattle, WA 98104 vtobias@u.washington.edu 206-744-2735 206-744-8240 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. From Richard.Cartun <@t> hhchealth.org Tue Sep 24 16:04:52 2013 From: Richard.Cartun <@t> hhchealth.org (Cartun, Richard) Date: Tue Sep 24 16:04:57 2013 Subject: [Histonet] RE: ER/PR results tracking In-Reply-To: References: Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E01886220@HHCEXCHMB05.hhcsystem.org> Hi Clare: Unfortunately, I have to do it manually using an Excel spreadsheet that I created. For every case we test (invasive, DCIS, and metastatic), I keep track of the specimen number, block designation, the time the tissue is placed in formalin, ER IHC result, PR IHC result, HER2 IHC result, HER2 FISH (if we do it and "we don't do many"), Oncotype DX result (if performed), and comments such as "repeat on excisional tumor", "S/P neoadjuvant chemotherapy (with or without Herceptin), "biopsy immunoprofile confirmed", etc. It's a lot of work, but I have exceptionally good data going back to 2011. For example, my positive rates for invasive carcinoma (114 cases) during the period from January-March, 2013 are: ER - 88.6% PR - 70.2% HER2 - 15.8% I just gave talks on this at the ASCP and NSH meetings. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax richard.cartun@hhchealth.org ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Clare Thornton [CThornton@dahlchase.com] Sent: Tuesday, September 24, 2013 2:40 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ER/PR results tracking How is everyone tracking their ER/PR results to comply with the following: ANP.22970 Annual Result Comparison Phase I For immunohistochemical and FISH/ISH tests that provide independent predictive information, the laboratory at least annually compares its patient results with published benchmarks, and evaluates interobserver variability among the pathologists in the laboratory. NOTE: Individuals interpreting the assay must also have their concordance compared with each other and this concordance should also be at least 95%. With specific reference to estrogen and progesterone receptor studies: in general, the overall proportion of ER-negative breast cancers (invasive and DCIS) should not exceed 30%. The average is somewhat lower in postmenopausal than premenopausal women (approximately 20% vs. 35%). The average is considerably lower in well-differentiated carcinomas (<10%) and certain special types of invasive carcinomas (<10% in lobular, tubular, and mucinous types). The proportion of PgR-negative cases is 10-15% higher than for ER in each of these settings. Investigation is warranted if the proportion of negative cases is significantly higher in any of these settings. Our LIS doesn't have an easy way of compiling these results. Basically, we are stuck with searching all cases with ER/PR done and manually reading and recording results. Does anyone have a good shortcut or idea of how to compile these results when your LIS doesn't keep track of the results for you? Thanks in advance! Clare J. Thornton, HTL(ASCP)QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From tony.henwood <@t> health.nsw.gov.au Tue Sep 24 18:09:15 2013 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Tue Sep 24 18:09:44 2013 Subject: [Histonet] RE: Peggy Wenks award In-Reply-To: References: Message-ID: <6D6BD1DE8A5571489398B392A38A71579D29FF73@xmdb04.nch.kids> Congratulations, well deserved from down-under Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Tuesday, 24 September 2013 10:57 PM To: 'Sue Hunter'; 'Histonet (histonet@lists.utsouthwestern.edu)'; histonet-bounces@lists.utsouthwestern.edu Subject: [Histonet] RE: Peggy Wenks award Congrats, Peggy. Very well deserved!! Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sue Hunter Sent: Tuesday, September 24, 2013 8:30 AM To: 'Histonet (histonet@lists.utsouthwestern.edu)'; histonet-bounces@lists.utsouthwestern.edu Subject: [Histonet] Peggy Wenks award For those of you who may not be attending the National Convention or did not hear, we at Beaumont are so proud to announce that Peggy "Amazing" Wenk has won the J.B. McCormick Award. This very prestigious award was presented by Dr. McCormick and was given to Peggy for her outstanding and exceptional service to the NSH. Please join us in congratulating Peggy on this well deserved recognition and award. Sue Sue Hunter, Supervisor Advanced Diagnostics Beaumont Health System Royal Oak MI 248-898-5146 shunter@beaumont.edu ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From histotalk <@t> yahoo.com Tue Sep 24 18:54:42 2013 From: histotalk <@t> yahoo.com (David Kemler) Date: Tue Sep 24 18:54:48 2013 Subject: [Histonet] RE: Peggy Wenks award In-Reply-To: <6D6BD1DE8A5571489398B392A38A71579D29FF73@xmdb04.nch.kids> References: <6D6BD1DE8A5571489398B392A38A71579D29FF73@xmdb04.nch.kids> Message-ID: <1380066882.48974.YahooMailNeo@web121503.mail.ne1.yahoo.com> I interviewed Peggy Sunday morning?at 8 am and she was as bubbly and humble as always. What an amazing lady!?More than likely, her 53 minute interview will be aired on HistoTALK http://www.histotalk.com/ the 2nd Sunday of October, the 14th. A most appropriate time.? ? Yours, Dave ________________________________ From: Tony Henwood (SCHN) To: "'Weems, Joyce K.'" ; 'Sue Hunter' ; "'Histonet (histonet@lists.utsouthwestern.edu)'" ; "histonet-bounces@lists.utsouthwestern.edu" Sent: Tuesday, September 24, 2013 7:09 PM Subject: [Histonet] RE: Peggy Wenks award Congratulations, well deserved from down-under Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Tuesday, 24 September 2013 10:57 PM To: 'Sue Hunter'; 'Histonet (histonet@lists.utsouthwestern.edu)'; histonet-bounces@lists.utsouthwestern.edu Subject: [Histonet] RE: Peggy Wenks award Congrats, Peggy. Very well deserved!! Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org http://www.saintjosephsatlanta.org/ 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s).? It may contain information that is privileged and confidential.? Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sue Hunter Sent: Tuesday, September 24, 2013 8:30 AM To: 'Histonet (histonet@lists.utsouthwestern.edu)'; histonet-bounces@lists.utsouthwestern.edu Subject: [Histonet] Peggy Wenks award For those of you who may not be attending the National Convention or did not hear, we at Beaumont are so proud to announce that? Peggy "Amazing" Wenk has won the J.B. McCormick Award.? This very prestigious award was presented by Dr. McCormick and was given to Peggy for her outstanding and exceptional service to the NSH. Please join us in congratulating Peggy on this well deserved recognition and award. Sue Sue Hunter, Supervisor Advanced Diagnostics Beaumont Health System Royal Oak MI 248-898-5146 shunter@beaumont.edu ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From thigginsht <@t> msn.com Wed Sep 25 07:37:50 2013 From: thigginsht <@t> msn.com (Tim Higgins) Date: Wed Sep 25 07:37:55 2013 Subject: [Histonet] edge effect on IHC In-Reply-To: References: Message-ID: Hey Mary, I would suggest observing your stainer to ensure the proper drop zones are being performed and not just dropping closest to the label. I used Dako for years and loved them but you do have to make sure it dispenses properly from time to time. They use drop zones unlike Ventana which uses a vortex type of mixing. Good luck! Thanks, Timothy N. Higgins, HT (ASCP), QIHC > Date: Mon, 23 Sep 2013 14:00:57 -0700 (PDT) > From: Mary Benoit > Subject: [Histonet] edge effect on IHC > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > <1379970057.43816.YahooMailNeo@web142505.mail.bf1.yahoo.com> > Content-Type: text/plain; charset=us-ascii > > I have been aware of some "edge effect" on an occasional slide, but lately we noticed the effect is showing up in the area of the slide that is farthest from the labels. We use DAKO Link Autostainer and their pretreatment baths for everything. One case in particular we stained a large melanoma met to colon that was well fixed and processed that stained only the edge, however when we sent block to Neogenomics reference lab, their slide stained evenly over the entire tumor area. We both used a melanoma cocktail. Not sure what is going on. Any suggestions? > Thank you > Mary F Benoit, MT(ASCP) > The Pathology Laboratory > 830 Bayou Pines Drive > Lake Charles, LA 70601 > mbplab@yahoo.com From shive003 <@t> umn.edu Wed Sep 25 09:58:06 2013 From: shive003 <@t> umn.edu (Jan Shivers) Date: Wed Sep 25 09:58:17 2013 Subject: [Histonet] edge effect on IHC In-Reply-To: References: Message-ID: Also make sure that the tissue never dries out on the trimming table before it even gets to formalin. That can also be a source of edge effect. Jan Shivers UMN On Wed, Sep 25, 2013 at 7:37 AM, Tim Higgins wrote: > Hey Mary, > > > > I would suggest observing your > stainer to ensure the proper drop zones are being performed and not just > dropping closest to the label. I used Dako for years and loved them but > you do > have to make sure it dispenses properly from time to time. They use drop > zones > unlike Ventana which uses a vortex type of mixing. > > > > Good luck! > > > > Thanks, > > > > Timothy N. Higgins, HT (ASCP), QIHC > > > > > > > Date: Mon, 23 Sep 2013 14:00:57 -0700 (PDT) > > From: Mary Benoit > > Subject: [Histonet] edge effect on IHC > > To: "histonet@lists.utsouthwestern.edu" > > > > Message-ID: > > <1379970057.43816.YahooMailNeo@web142505.mail.bf1.yahoo.com> > > Content-Type: text/plain; charset=us-ascii > > > > I have been aware of some "edge effect" on an occasional slide, but > lately we noticed the effect is showing up in the area of the slide that is > farthest from the labels. We use DAKO Link Autostainer and their > pretreatment baths for everything. One case in particular we stained a > large melanoma met to colon that was well fixed and processed that stained > only the edge, however when we sent block to Neogenomics reference lab, > their slide stained evenly over the entire tumor area. We both used a > melanoma cocktail. Not sure what is going on. Any suggestions? > > Thank you > > Mary F Benoit, MT(ASCP) > > The Pathology Laboratory > > 830 Bayou Pines Drive > > Lake Charles, LA 70601 > > mbplab@yahoo.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From reynaniteraven <@t> gmail.com Wed Sep 25 10:15:46 2013 From: reynaniteraven <@t> gmail.com (Jackie Lair) Date: Wed Sep 25 10:11:49 2013 Subject: [Histonet] RE: Peggy Wenks award In-Reply-To: <6D6BD1DE8A5571489398B392A38A71579D29FF73@xmdb04.nch.kids> References: <6D6BD1DE8A5571489398B392A38A71579D29FF73@xmdb04.nch.kids> Message-ID: <6B319F96-AAFF-487D-9292-30C873758BEA@gmail.com> Congrats Peggy!!! :) Jackie Lair Sent from my iPhone On Sep 24, 2013, at 4:09 PM, "Tony Henwood (SCHN)" wrote: > Congratulations, well deserved from down-under > > Regards > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) > Laboratory Manager & Senior Scientist > Tel: 612 9845 3306 > Fax: 612 9845 3318 > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead > Locked Bag 4001, Westmead NSW 2145, AUSTRALIA > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. > Sent: Tuesday, 24 September 2013 10:57 PM > To: 'Sue Hunter'; 'Histonet (histonet@lists.utsouthwestern.edu)'; histonet-bounces@lists.utsouthwestern.edu > Subject: [Histonet] RE: Peggy Wenks award > > Congrats, Peggy. Very well deserved!! > > Joyce Weems > Pathology Manager > 678-843-7376 Phone > 678-843-7831 Fax > joyce.weems@emoryhealthcare.org > > > > www.saintjosephsatlanta.org > 5665 Peachtree Dunwoody Road > Atlanta, GA 30342 > > This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sue Hunter > Sent: Tuesday, September 24, 2013 8:30 AM > To: 'Histonet (histonet@lists.utsouthwestern.edu)'; histonet-bounces@lists.utsouthwestern.edu > Subject: [Histonet] Peggy Wenks award > > For those of you who may not be attending the National Convention or did not hear, we at Beaumont are so proud to announce that Peggy "Amazing" Wenk has won the J.B. McCormick Award. This very prestigious award was presented by Dr. McCormick and was given to Peggy for her outstanding and exceptional service to the NSH. > Please join us in congratulating Peggy on this well deserved recognition and award. > Sue > > Sue Hunter, Supervisor > Advanced Diagnostics > Beaumont Health System > Royal Oak MI > 248-898-5146 > shunter@beaumont.edu > > > > ________________________________ > > This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. > > If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ********************************************************************************* > This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. > > Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. > > This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. > ********************************************************************************* > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From claycal44 <@t> yahoo.com Wed Sep 25 10:49:03 2013 From: claycal44 <@t> yahoo.com (nancy lowen) Date: Wed Sep 25 10:49:09 2013 Subject: [Histonet] RE: Submitting Questions In-Reply-To: Message-ID: <1380124143.69165.YahooMailBasic@web125606.mail.ne1.yahoo.com> Me too! -------------------------------------------- On Tue, 9/24/13, Martha Ward-Pathology wrote: Subject: [Histonet] RE: Submitting Questions To: "Nails, Felton" , "'Joanne Clark'" , "histonet@lists.utsouthwestern.edu" Date: Tuesday, September 24, 2013, 12:54 PM I have had the same problem over the past few months... ? Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard ?\? Winston-Salem, NC 27157 p 336.716.2109 ?\? f 336.716.5890 ? mward@wakehealth.edu ? ? ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nails, Felton Sent: Tuesday, September 24, 2013 2:58 PM To: 'Joanne Clark'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Submitting Questions I am in the same situation??? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joanne Clark Sent: Tuesday, September 24, 2013 12:54 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Submitting Questions Can anyone out in histoland explain to me why my submitted questions are not making it through to histonet?? I submitted a question on the 19th of September, and I still have not seen it appear in the group emails I get daily.? I sent the question to the correct address (I checked it to be sure).? When I respond to someones question, I can see my responses, I just can't seem to get an original question submitted.? Can anyone tell me what I am doing wrong? Disclaimer: This electronic message may contain information that is proprietary, confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or? privileged.? If you are not the intended recipient or an? authorized representative of the intended recipient, you? are hereby notified that any review, dissemination, or? copying of this e-mail and its attachments, if any, or? the information contained herein is prohibited.? If you? have received this e-mail in error, please immediately? notify the sender by return e-mail and delete this e-mail? from your computer system.? Thank you. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bbrinegarhtl <@t> gmail.com Wed Sep 25 10:56:58 2013 From: bbrinegarhtl <@t> gmail.com (Beth Brinegar) Date: Wed Sep 25 10:57:07 2013 Subject: [Histonet] QIHC Message-ID: Hello Histoland! I have been an HTL for about a year and a half now and I'm looking to go for my QIHC - any suggestions for studying? What are the best resources for study? Are there any good websites or practice questions? Any study tips or must-have resources? I've looked at the ASCP website and I have most of the books on there plus some others that were handed down to me. Any assistance would be greatly appreciated!! Thanks! Beth Brinegar HTL(ASCP) Anatomic Pathology Supervisor Mercy Medical Center Cedar Rapids, IA 52403 From flnails <@t> texaschildrens.org Wed Sep 25 11:05:23 2013 From: flnails <@t> texaschildrens.org (Nails, Felton) Date: Wed Sep 25 11:07:55 2013 Subject: [Histonet] RE: Submitting Questions In-Reply-To: <1380124143.69165.YahooMailBasic@web125606.mail.ne1.yahoo.com> References: <1380124143.69165.YahooMailBasic@web125606.mail.ne1.yahoo.com> Message-ID: <327E034F1892504289B7A17EC71DF9F303BBC6@TCFMSG03.ad.texaschildrenshospital.org> I have even tried copying the following link (histonet@lists.utsouthwestern.edu) and pasting it and I'm still unable to make a post. -----Original Message----- From: nancy lowen [mailto:claycal44@yahoo.com] Sent: Wednesday, September 25, 2013 10:49 AM To: Nails, Felton; 'Joanne Clark'; histonet@lists.utsouthwestern.edu; Martha Ward-Pathology Subject: Re: [Histonet] RE: Submitting Questions Me too! -------------------------------------------- On Tue, 9/24/13, Martha Ward-Pathology wrote: Subject: [Histonet] RE: Submitting Questions To: "Nails, Felton" , "'Joanne Clark'" , "histonet@lists.utsouthwestern.edu" Date: Tuesday, September 24, 2013, 12:54 PM I have had the same problem over the past few months... ? Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard ?\? Winston-Salem, NC 27157 p 336.716.2109 ?\? f 336.716.5890 mward@wakehealth.edu ? ? ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nails, Felton Sent: Tuesday, September 24, 2013 2:58 PM To: 'Joanne Clark'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Submitting Questions I am in the same situation??? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joanne Clark Sent: Tuesday, September 24, 2013 12:54 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Submitting Questions Can anyone out in histoland explain to me why my submitted questions are not making it through to histonet?? I submitted a question on the 19th of September, and I still have not seen it appear in the group emails I get daily.? I sent the question to the correct address (I checked it to be sure).? When I respond to someones question, I can see my responses, I just can't seem to get an original question submitted.? Can anyone tell me what I am doing wrong? Disclaimer: This electronic message may contain information that is proprietary, confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or? privileged.? If you are not the intended recipient or an? authorized representative of the intended recipient, you? are hereby notified that any review, dissemination, or? copying of this e-mail and its attachments, if any, or? the information contained herein is prohibited.? If you? have received this e-mail in error, please immediately? notify the sender by return e-mail and delete this e-mail? from your computer system.? Thank you. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ______________________________________________________________________ From BDeBrosse-Serra <@t> isisph.com Wed Sep 25 11:10:06 2013 From: BDeBrosse-Serra <@t> isisph.com (Bea DeBrosse-Serra) Date: Wed Sep 25 11:10:11 2013 Subject: [Histonet] QIHC In-Reply-To: References: Message-ID: Dako IHC hand book. Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Beth Brinegar Sent: Wednesday, September 25, 2013 8:57 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] QIHC Hello Histoland! I have been an HTL for about a year and a half now and I'm looking to go for my QIHC - any suggestions for studying? What are the best resources for study? Are there any good websites or practice questions? Any study tips or must-have resources? I've looked at the ASCP website and I have most of the books on there plus some others that were handed down to me. Any assistance would be greatly appreciated!! Thanks! Beth Brinegar HTL(ASCP) Anatomic Pathology Supervisor Mercy Medical Center Cedar Rapids, IA 52403 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From doug.porter <@t> caplab.org Wed Sep 25 11:20:19 2013 From: doug.porter <@t> caplab.org (Douglas Porter) Date: Wed Sep 25 11:18:23 2013 Subject: [Histonet] RE: Submitting Questions In-Reply-To: <327E034F1892504289B7A17EC71DF9F303BBC6@TCFMSG03.ad.texaschildrenshospital.org> References: <1380124143.69165.YahooMailBasic@web125606.mail.ne1.yahoo.com> <327E034F1892504289B7A17EC71DF9F303BBC6@TCFMSG03.ad.texaschildrenshospital.org> Message-ID: <002601ceba0b$218f7760$64ae6620$@caplab.org> I have .jpg files in my signature for initial emails but not in my reply emails. I sent a test message yesterday and got the following reply from the server: Your mail to 'Histonet' with the subject Test Message! Is being held until the list moderator can review it for approval. The reason it is being held: Message body is too big: 119656 bytes with a limit of 60 KB Either the message will get posted to the list, or you will receive notification of the moderator's decision. If you would like to cancel this posting, please visit the following URL: http://lists.utsouthwestern.edu/mailman/confirm/histonet/9abe483614aeb7cc29e e5aa890b57bc164701b31 I suspect the .jpg files are what is causing the problem as the message was only "Test Message!" I will strip out the .jpg files and try again. Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.porter@caplab.org www.caplab.org ? ? The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, copying, forwarding or capture of this communication is strictly prohibited. If you have received this communication in error, please notify me immediately by return e-mail and delete this and all copies. Thank-you. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nails, Felton Sent: Wednesday, September 25, 2013 12:05 PM To: 'nancy lowen'; 'Joanne Clark'; histonet@lists.utsouthwestern.edu; Martha Ward-Pathology Subject: RE: [Histonet] RE: Submitting Questions I have even tried copying the following link (histonet@lists.utsouthwestern.edu) and pasting it and I'm still unable to make a post. -----Original Message----- From: nancy lowen [mailto:claycal44@yahoo.com] Sent: Wednesday, September 25, 2013 10:49 AM To: Nails, Felton; 'Joanne Clark'; histonet@lists.utsouthwestern.edu; Martha Ward-Pathology Subject: Re: [Histonet] RE: Submitting Questions Me too! -------------------------------------------- On Tue, 9/24/13, Martha Ward-Pathology wrote: Subject: [Histonet] RE: Submitting Questions To: "Nails, Felton" , "'Joanne Clark'" , "histonet@lists.utsouthwestern.edu" Date: Tuesday, September 24, 2013, 12:54 PM I have had the same problem over the past few months... ? Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard ?\? Winston-Salem, NC 27157 p 336.716.2109 ?\? f 336.716.5890 mward@wakehealth.edu ? ? ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nails, Felton Sent: Tuesday, September 24, 2013 2:58 PM To: 'Joanne Clark'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Submitting Questions I am in the same situation??? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joanne Clark Sent: Tuesday, September 24, 2013 12:54 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Submitting Questions Can anyone out in histoland explain to me why my submitted questions are not making it through to histonet?? I submitted a question on the 19th of September, and I still have not seen it appear in the group emails I get daily.? I sent the question to the correct address (I checked it to be sure).? When I respond to someones question, I can see my responses, I just can't seem to get an original question submitted.? Can anyone tell me what I am doing wrong? Disclaimer: This electronic message may contain information that is proprietary, confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or? privileged.? If you are not the intended recipient or an? authorized representative of the intended recipient, you? are hereby notified that any review, dissemination, or? copying of this e-mail and its attachments, if any, or? the information contained herein is prohibited.? If you? have received this e-mail in error, please immediately? notify the sender by return e-mail and delete this e-mail? from your computer system.? Thank you. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From doug.porter <@t> caplab.org Wed Sep 25 11:22:21 2013 From: doug.porter <@t> caplab.org (Douglas Porter) Date: Wed Sep 25 11:21:36 2013 Subject: [Histonet] Test message w/o .jpg files! Message-ID: <002701ceba0b$6a1794e0$3e46bea0$@caplab.org> Test message w/o .jpg files in email signature. Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.porter@caplab.org www.caplab.org The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, copying, forwarding or capture of this communication is strictly prohibited. If you have received this communication in error, please notify me immediately by return e-mail and delete this and all copies. Thank-you. From doug.porter <@t> caplab.org Wed Sep 25 11:31:01 2013 From: doug.porter <@t> caplab.org (Douglas Porter) Date: Wed Sep 25 11:29:01 2013 Subject: [Histonet] Test message w/o .jpg files! In-Reply-To: <002701ceba0b$6a1794e0$3e46bea0$@caplab.org> References: <002701ceba0b$6a1794e0$3e46bea0$@caplab.org> Message-ID: <002c01ceba0c$9fcfe960$df6fbc20$@caplab.org> This message posted within five minutes of sending it. The one from yesterday with the .jpg files in the email signature I suspect will never be posted. Thus ends the experiment. If you want to make sure your emails get posted, make sure you have no pictures/icons in your signature. Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.porter@caplab.org www.caplab.org ? ? The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, copying, forwarding or capture of this communication is strictly prohibited. If you have received this communication in error, please notify me immediately by return e-mail and delete this and all copies. Thank-you. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas Porter Sent: Wednesday, September 25, 2013 12:22 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Test message w/o .jpg files! Test message w/o .jpg files in email signature. Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.porter@caplab.org www.caplab.org The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, copying, forwarding or capture of this communication is strictly prohibited. If you have received this communication in error, please notify me immediately by return e-mail and delete this and all copies. Thank-you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Wed Sep 25 11:50:07 2013 From: joelleweaver <@t> hotmail.com (=?utf-8?B?am9lbGxld2VhdmVyQGhvdG1haWwuY29t?=) Date: Wed Sep 25 11:49:42 2013 Subject: =?utf-8?B?UmU6IFtIaXN0b25ldF0gUUlIQw==?= Message-ID: Ditto,Dako on website ad pdf Sent from my Verizon Wireless 4G LTE Smartphone ----- Reply message ----- From: "Bea DeBrosse-Serra" To: "'Beth Brinegar'" , "histonet@lists.utsouthwestern.edu" Subject: [Histonet] QIHC Date: Wed, Sep 25, 2013 12:10 pm Dako IHC hand book. Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Beth Brinegar Sent: Wednesday, September 25, 2013 8:57 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] QIHC Hello Histoland! I have been an HTL for about a year and a half now and I'm looking to go for my QIHC - any suggestions for studying? What are the best resources for study? Are there any good websites or practice questions? Any study tips or must-have resources? I've looked at the ASCP website and I have most of the books on there plus some others that were handed down to me. Any assistance would be greatly appreciated!! Thanks! Beth Brinegar HTL(ASCP) Anatomic Pathology Supervisor Mercy Medical Center Cedar Rapids, IA 52403 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Joyce.Weems <@t> emoryhealthcare.org Wed Sep 25 11:51:33 2013 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Wed Sep 25 11:51:42 2013 Subject: [Histonet] Test message w/o .jpg files! In-Reply-To: <002701ceba0b$6a1794e0$3e46bea0$@caplab.org> References: <002701ceba0b$6a1794e0$3e46bea0$@caplab.org> Message-ID: Thank you for figuring this out!! Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas Porter Sent: Wednesday, September 25, 2013 12:22 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Test message w/o .jpg files! Test message w/o .jpg files in email signature. Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.porter@caplab.org www.caplab.org The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, copying, forwarding or capture of this communication is strictly prohibited. If you have received this communication in error, please notify me immediately by return e-mail and delete this and all copies. Thank-you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From flnails <@t> texaschildrens.org Wed Sep 25 12:03:17 2013 From: flnails <@t> texaschildrens.org (Nails, Felton) Date: Wed Sep 25 12:03:42 2013 Subject: [Histonet] test Message-ID: <327E034F1892504289B7A17EC71DF9F303BE06@TCFMSG03.ad.texaschildrenshospital.org> ______________________________________________________________________ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ______________________________________________________________________ From joelleweaver <@t> hotmail.com Wed Sep 25 12:15:54 2013 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Wed Sep 25 12:15:59 2013 Subject: [Histonet] QIHC In-Reply-To: References: Message-ID: geez, sorry my messages are posting twice. Joelle Weaver MAOM, HTL (ASCP) QIHC To: BDeBrosse-Serra@isisph.com; bbrinegarhtl@gmail.com; histonet@lists.utsouthwestern.edu From: joelleweaver@hotmail.com Subject: Re: [Histonet] QIHC Date: Wed, 25 Sep 2013 12:50:07 -0400 Ditto,Dako on website ad pdf Sent from my Verizon Wireless 4G LTE Smartphone ----- Reply message ----- From: "Bea DeBrosse-Serra" To: "'Beth Brinegar'" , "histonet@lists.utsouthwestern.edu" Subject: [Histonet] QIHC Date: Wed, Sep 25, 2013 12:10 pm Dako IHC hand book. Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Beth Brinegar Sent: Wednesday, September 25, 2013 8:57 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] QIHC Hello Histoland! I have been an HTL for about a year and a half now and I'm looking to go for my QIHC - any suggestions for studying? What are the best resources for study? Are there any good websites or practice questions? Any study tips or must-have resources? I've looked at the ASCP website and I have most of the books on there plus some others that were handed down to me. Any assistance would be greatly appreciated!! Thanks! Beth Brinegar HTL(ASCP) Anatomic Pathology Supervisor Mercy Medical Center Cedar Rapids, IA 52403 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From thomas.huynh <@t> mdanderson.org Wed Sep 25 13:06:54 2013 From: thomas.huynh <@t> mdanderson.org (Huynh,Thomas) Date: Wed Sep 25 13:07:00 2013 Subject: [Histonet] Powerpath QA Message-ID: Ms. McCaig We are using it for quite some time; it is not the best out there but if you can contact me, I would be able to go into detail further. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Wednesday, September 25, 2013 10:53 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 118, Issue 43 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Submitting Questions (Joanne Clark) 2. ER/PR results tracking (Clare Thornton) 3. RE: Submitting Questions (Nails, Felton) 4. Ceramide antibodies (Mark Elliott) 5. RE: RE: Submitting Questions (Douglas Porter) 6. Article: Anatomic Pathology Takes a Leap in Quality Process Improvement (Judy O'Rourke) 7. Re: Histonet Digest, Vol 118, Issue 42 (Mark Elliott) 8. RE: Submitting Questions (Martha Ward-Pathology) 9. RE: RE: Submitting Questions (pruegg@ihctech.net) 10. PowerPath QA (Diana McCaig) 11. Ventana Vantage and PowerPath (Victor A. Tobias) 12. RE: ER/PR results tracking (Cartun, Richard) 13. RE: Peggy Wenks award (Tony Henwood (SCHN)) 14. Re: RE: Peggy Wenks award (David Kemler) 15. RE: edge effect on IHC (Tim Higgins) 16. Re: edge effect on IHC (Jan Shivers) 17. Re: RE: Peggy Wenks award (Jackie Lair) 18. Re: RE: Submitting Questions (nancy lowen) ---------------------------------------------------------------------- Message: 1 Date: Tue, 24 Sep 2013 17:53:56 +0000 From: Joanne Clark Subject: [Histonet] Submitting Questions To: "histonet@lists.utsouthwestern.edu" Message-ID: <0494A7D4E8CC254EA2FB81464982E378B4ABA154@S10MAILD001N3.SH10.lan> Content-Type: text/plain; charset="us-ascii" Can anyone out in histoland explain to me why my submitted questions are not making it through to histonet? I submitted a question on the 19th of September, and I still have not seen it appear in the group emails I get daily. I sent the question to the correct address (I checked it to be sure). When I respond to someones question, I can see my responses, I just can't seem to get an original question submitted. Can anyone tell me what I am doing wrong? Disclaimer: This electronic message may contain information that is proprietary, confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ------------------------------ Message: 2 Date: Tue, 24 Sep 2013 14:40:15 -0400 From: Clare Thornton Subject: [Histonet] ER/PR results tracking To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" How is everyone tracking their ER/PR results to comply with the following: ANP.22970 Annual Result Comparison Phase I For immunohistochemical and FISH/ISH tests that provide independent predictive information, the laboratory at least annually compares its patient results with published benchmarks, and evaluates interobserver variability among the pathologists in the laboratory. NOTE: Individuals interpreting the assay must also have their concordance compared with each other and this concordance should also be at least 95%. With specific reference to estrogen and progesterone receptor studies: in general, the overall proportion of ER-negative breast cancers (invasive and DCIS) should not exceed 30%. The average is somewhat lower in postmenopausal than premenopausal women (approximately 20% vs. 35%). The average is considerably lower in well-differentiated carcinomas (<10%) and certain special types of invasive carcinomas (<10% in lobular, tubular, and mucinous types). The proportion of PgR-negative cases is 10-15% higher than for ER in each of these settings. Investigation is warranted if the proportion of negative cases is significantly higher in any of these settings. Our LIS doesn't have an easy way of compiling these results. Basically, we are stuck with searching all cases with ER/PR done and manually reading and recording results. Does anyone have a good shortcut or idea of how to compile these results when your LIS doesn't keep track of the results for you? Thanks in advance! Clare J. Thornton, HTL(ASCP)QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com ------------------------------ Message: 3 Date: Tue, 24 Sep 2013 18:57:44 +0000 From: "Nails, Felton" Subject: [Histonet] RE: Submitting Questions To: 'Joanne Clark' , "histonet@lists.utsouthwestern.edu" Message-ID: <327E034F1892504289B7A17EC71DF9F303B9D1@TCFMSG03.ad.texaschildrenshospital.org> Content-Type: text/plain; charset="us-ascii" I am in the same situation??? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joanne Clark Sent: Tuesday, September 24, 2013 12:54 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Submitting Questions Can anyone out in histoland explain to me why my submitted questions are not making it through to histonet? I submitted a question on the 19th of September, and I still have not seen it appear in the group emails I get daily. I sent the question to the correct address (I checked it to be sure). When I respond to someones question, I can see my responses, I just can't seem to get an original question submitted. Can anyone tell me what I am doing wrong? Disclaimer: This electronic message may contain information that is proprietary, confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ______________________________________________________________________ ------------------------------ Message: 4 Date: Tue, 24 Sep 2013 12:09:04 -0700 From: "Mark Elliott" Subject: [Histonet] Ceramide antibodies To: Message-ID: <524180E0020000D6000659E5@mail.hli.ubc.ca> Content-Type: text/plain; charset="us-ascii" I have been asked to do some staining for ceramide in human lungs. Anyone have recommendations for an antibody that works well on human tissues? Any tips would be greatly appreciated. This is for either frozen tissue or FFPE. Thanks Mark ------------------------------ Message: 5 Date: Tue, 24 Sep 2013 15:11:25 -0400 From: "Douglas Porter" Subject: RE: [Histonet] RE: Submitting Questions To: "'Nails, Felton'" , Message-ID: <003701ceb959$ddea70c0$99bf5240$@caplab.org> Content-Type: text/plain; charset="iso-8859-1" Check to make sure your Histonet email address is correct. When you reply to one the email is already entered. That may be why you can see your responses and not your original messages. Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.porter@caplab.org www.caplab.org ? ? The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, copying, forwarding or capture of this communication is strictly prohibited. If you have received this communication in error, please notify me immediately by return e-mail and delete this and all copies. Thank-you. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nails, Felton Sent: Tuesday, September 24, 2013 2:58 PM To: 'Joanne Clark'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Submitting Questions I am in the same situation??? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joanne Clark Sent: Tuesday, September 24, 2013 12:54 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Submitting Questions Can anyone out in histoland explain to me why my submitted questions are not making it through to histonet? I submitted a question on the 19th of September, and I still have not seen it appear in the group emails I get daily. I sent the question to the correct address (I checked it to be sure). When I respond to someones question, I can see my responses, I just can't seem to get an original question submitted. Can anyone tell me what I am doing wrong? Disclaimer: This electronic message may contain information that is proprietary, confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Tue, 24 Sep 2013 19:15:23 +0000 From: Judy O'Rourke Subject: [Histonet] Article: Anatomic Pathology Takes a Leap in Quality Process Improvement To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" William DeSalvo, BS HTL(ASCP), has written the cover article in CLP's September issue: Anatomic Pathology Takes a Leap in Quality Process Improvement Found here in the digital edition (page 8): http://edition.pagesuite-professional.co.uk//launch.aspx?pbid=db6b2441-3876-40c8-8eb3-8a480a616c4e Regards, Judy O'Rourke Editor/CLP ------------------------------ Message: 7 Date: Tue, 24 Sep 2013 12:40:58 -0700 From: "Mark Elliott" Subject: [Histonet] Re: Histonet Digest, Vol 118, Issue 42 To: Message-ID: <5241885A020000D600065A10@mail.hli.ubc.ca> Content-Type: text/plain; charset="us-ascii" Well done Peggy!!! Mark ------------------------------ Message: 8 Date: Tue, 24 Sep 2013 19:54:09 +0000 From: Martha Ward-Pathology Subject: [Histonet] RE: Submitting Questions To: "Nails, Felton" , 'Joanne Clark' , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" I have had the same problem over the past few months... ? Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard ?\? Winston-Salem, NC 27157 p 336.716.2109 ?\? f 336.716.5890 ? mward@wakehealth.edu ? ? ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nails, Felton Sent: Tuesday, September 24, 2013 2:58 PM To: 'Joanne Clark'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Submitting Questions I am in the same situation??? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joanne Clark Sent: Tuesday, September 24, 2013 12:54 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Submitting Questions Can anyone out in histoland explain to me why my submitted questions are not making it through to histonet? I submitted a question on the 19th of September, and I still have not seen it appear in the group emails I get daily. I sent the question to the correct address (I checked it to be sure). When I respond to someones question, I can see my responses, I just can't seem to get an original question submitted. Can anyone tell me what I am doing wrong? Disclaimer: This electronic message may contain information that is proprietary, confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Tue, 24 Sep 2013 13:44:18 -0700 From: Subject: RE: [Histonet] RE: Submitting Questions To: "Martha Ward-Pathology" , "Nails, Felton" , "'Joanne Clark'" , "histonet@lists.utsouthwestern.edu" Message-ID: <20130924134418.f86bd30e73b823f57b516b5451216a98.3f1c6353d6.wbe@email01.secureserver.net> Content-Type: text/plain; charset="utf-8" do u see this question posted? -------- Original Message --------< Submitting Questions From: Martha Ward- Date: Tue, September 24, 2013 12:54 pm To: "Nails, Felt Clark' <[3] <[5]histonet@lists.utsouthwestern.edu> I have had the same problem over the past few months... &n Martha Ward, MT (ASCP) QIHC Manager Molecular Medical Center Boulevard \ Winston-Salem p 336.716.2109 \ f 336.716.5890 < -----Original Message---- From: [6] [[7]mailto:histonet-bounces@lists.utsouthweste Nails, Felton Sent: Tuesday, September 24, 2 To: 'Joanne Clark'; [8]histonet@lists.utsouthwestern.edu Subject: [Histo I am in the same situation??? -----Original Message----- From: [9]histonet-bounces@lists.utsouthwestern. o:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joanne Clark< To: [11]histonet@lists.utsouthwestern.edu< [Histonet] Submitting Questions Can anyone out questions are not making it thr question on the 19th of September, and I s appear in the group emails I get daily. I sent the q correct address (I checked it to be sure). When I respond t someones question, I can see my responses, I just can't seem to get an or wrong? Disclaimer: This electronic message may cont is proprietary, confidential, or legally privileged or is intended only for the use of the individual(s) and entity the message. If you are not an intended recipient of this message please notify the sender immediately and delete the material from your co disclose information it contains _______________________________________________ Histon [12] [13]http://lists.utsouthwestern.edu/mail ____________________________________ __________________________________ CONFIDENTIALITY NOTICE: The privileged. If you representative of the int that any review, dissemination, o attachments, if any, or the information prohibited. If you have received this e-mail in error immediately notify the sender by return e-mail and delete this e- mail from your computer system. Thank you. ________________________ ______________________________________________ _______________ Histonet mailing list [14]Histonet@lists.utsouthwestern [15]http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Hist [16]Histonet@lists.utsouthwestern.edu [17]http://lists.utsouthwestern.edu/ma References 1. 3D"mailto:mward@wakehealth.edu" 2. 3D"mailto:flnails@texaschildrens.org" 3. 3D"mailto:jclark@pcnm.com" 4. 3D"mailto:histonet@lists.utsouthwestern.e 5. 3D"mailto:hist 6. 3D"mailto:histonet-bounces@lists.utsouthwestern.edu" 7. 3D"mailto:histonet-b 8. 3D"mailto:histonet@lists.utso 9. 3D"mailto:histon 10. 3D"mailto:histonet-bounces@lists.utsouthwestern.edu" 11. file://localhost/tmp/3D"mailt 12. 3D"mailto:Histonet@lists.utsouthwestern.edu" 13. 3D"http://lists.utsouth=/ 14. ="mailto:Histonet@lists.utsouthwestern.edu" 15. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/ 16. 3D"mailto:Histonet@lists.utsouthwestern.edu 17. 3D"http://lists.utsou=/ ------------------------------ Message: 10 Date: Tue, 24 Sep 2013 20:55:40 +0000 From: Diana McCaig Subject: [Histonet] PowerPath QA To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Is anyone using this module and are you willing to share any documents regarding it. Or opinions on what think of it for a QA program Sincere thanks Diana ------------------------------ Message: 11 Date: Tue, 24 Sep 2013 20:56:26 +0000 From: "Victor A. Tobias" Subject: [Histonet] Ventana Vantage and PowerPath To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Is anyone out there using this combination of software? If so, please contact me directly. Thanks Victor Victor Tobias HT(ASCP) Clinical Applications Analyst Harborview Medical Center Dept of Pathology Room NJB 244 Ninth & Jefferson Seattle, WA 98104 vtobias@u.washington.edu 206-744-2735 206-744-8240 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. ------------------------------ Message: 12 Date: Tue, 24 Sep 2013 21:04:52 +0000 From: "Cartun, Richard" Subject: [Histonet] RE: ER/PR results tracking To: Clare Thornton , "histonet@lists.utsouthwestern.edu" Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E01886220@HHCEXCHMB05.hhcsystem.org> Content-Type: text/plain; charset="us-ascii" Hi Clare: Unfortunately, I have to do it manually using an Excel spreadsheet that I created. For every case we test (invasive, DCIS, and metastatic), I keep track of the specimen number, block designation, the time the tissue is placed in formalin, ER IHC result, PR IHC result, HER2 IHC result, HER2 FISH (if we do it and "we don't do many"), Oncotype DX result (if performed), and comments such as "repeat on excisional tumor", "S/P neoadjuvant chemotherapy (with or without Herceptin), "biopsy immunoprofile confirmed", etc. It's a lot of work, but I have exceptionally good data going back to 2011. For example, my positive rates for invasive carcinoma (114 cases) during the period from January-March, 2013 are: ER - 88.6% PR - 70.2% HER2 - 15.8% I just gave talks on this at the ASCP and NSH meetings. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax richard.cartun@hhchealth.org ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Clare Thornton [CThornton@dahlchase.com] Sent: Tuesday, September 24, 2013 2:40 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ER/PR results tracking How is everyone tracking their ER/PR results to comply with the following: ANP.22970 Annual Result Comparison Phase I For immunohistochemical and FISH/ISH tests that provide independent predictive information, the laboratory at least annually compares its patient results with published benchmarks, and evaluates interobserver variability among the pathologists in the laboratory. NOTE: Individuals interpreting the assay must also have their concordance compared with each other and this concordance should also be at least 95%. With specific reference to estrogen and progesterone receptor studies: in general, the overall proportion of ER-negative breast cancers (invasive and DCIS) should not exceed 30%. The average is somewhat lower in postmenopausal than premenopausal women (approximately 20% vs. 35%). The average is considerably lower in well-differentiated carcinomas (<10%) and certain special types of invasive carcinomas (<10% in lobular, tubular, and mucinous types). The proportion of PgR-negative cases is 10-15% higher than for ER in each of these settings. Investigation is warranted if the proportion of negative cases is significantly higher in any of these settings. Our LIS doesn't have an easy way of compiling these results. Basically, we are stuck with searching all cases with ER/PR done and manually reading and recording results. Does anyone have a good shortcut or idea of how to compile these results when your LIS doesn't keep track of the results for you? Thanks in advance! Clare J. Thornton, HTL(ASCP)QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. ------------------------------ Message: 13 Date: Tue, 24 Sep 2013 23:09:15 +0000 From: "Tony Henwood (SCHN)" Subject: [Histonet] RE: Peggy Wenks award To: "'Weems, Joyce K.'" , "'Sue Hunter'" , "'Histonet (histonet@lists.utsouthwestern.edu)'" , "histonet-bounces@lists.utsouthwestern.edu" Message-ID: <6D6BD1DE8A5571489398B392A38A71579D29FF73@xmdb04.nch.kids> Content-Type: text/plain; charset="us-ascii" Congratulations, well deserved from down-under Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Tuesday, 24 September 2013 10:57 PM To: 'Sue Hunter'; 'Histonet (histonet@lists.utsouthwestern.edu)'; histonet-bounces@lists.utsouthwestern.edu Subject: [Histonet] RE: Peggy Wenks award Congrats, Peggy. Very well deserved!! Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sue Hunter Sent: Tuesday, September 24, 2013 8:30 AM To: 'Histonet (histonet@lists.utsouthwestern.edu)'; histonet-bounces@lists.utsouthwestern.edu Subject: [Histonet] Peggy Wenks award For those of you who may not be attending the National Convention or did not hear, we at Beaumont are so proud to announce that Peggy "Amazing" Wenk has won the J.B. McCormick Award. This very prestigious award was presented by Dr. McCormick and was given to Peggy for her outstanding and exceptional service to the NSH. Please join us in congratulating Peggy on this well deserved recognition and award. Sue Sue Hunter, Supervisor Advanced Diagnostics Beaumont Health System Royal Oak MI 248-898-5146 shunter@beaumont.edu ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* ------------------------------ Message: 14 Date: Tue, 24 Sep 2013 16:54:42 -0700 (PDT) From: David Kemler Subject: Re: [Histonet] RE: Peggy Wenks award To: Fellow HistoNetters Message-ID: <1380066882.48974.YahooMailNeo@web121503.mail.ne1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I interviewed Peggy Sunday morning?at 8 am and she was as bubbly and humble as always. What an amazing lady!?More than likely, her 53 minute interview will be aired on HistoTALK http://www.histotalk.com/ the 2nd Sunday of October, the 14th. A most appropriate time.? ? Yours, Dave ________________________________ From: Tony Henwood (SCHN) To: "'Weems, Joyce K.'" ; 'Sue Hunter' ; "'Histonet (histonet@lists.utsouthwestern.edu)'" ; "histonet-bounces@lists.utsouthwestern.edu" Sent: Tuesday, September 24, 2013 7:09 PM Subject: [Histonet] RE: Peggy Wenks award Congratulations, well deserved from down-under Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Tuesday, 24 September 2013 10:57 PM To: 'Sue Hunter'; 'Histonet (histonet@lists.utsouthwestern.edu)'; histonet-bounces@lists.utsouthwestern.edu Subject: [Histonet] RE: Peggy Wenks award Congrats, Peggy. Very well deserved!! Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org http://www.saintjosephsatlanta.org/ 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s).? It may contain information that is privileged and confidential.? Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sue Hunter Sent: Tuesday, September 24, 2013 8:30 AM To: 'Histonet (histonet@lists.utsouthwestern.edu)'; histonet-bounces@lists.utsouthwestern.edu Subject: [Histonet] Peggy Wenks award For those of you who may not be attending the National Convention or did not hear, we at Beaumont are so proud to announce that? Peggy "Amazing" Wenk has won the J.B. McCormick Award.? This very prestigious award was presented by Dr. McCormick and was given to Peggy for her outstanding and exceptional service to the NSH. Please join us in congratulating Peggy on this well deserved recognition and award. Sue Sue Hunter, Supervisor Advanced Diagnostics Beaumont Health System Royal Oak MI 248-898-5146 shunter@beaumont.edu ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Wed, 25 Sep 2013 07:37:50 -0500 From: Tim Higgins Subject: RE: [Histonet] edge effect on IHC To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hey Mary, I would suggest observing your stainer to ensure the proper drop zones are being performed and not just dropping closest to the label. I used Dako for years and loved them but you do have to make sure it dispenses properly from time to time. They use drop zones unlike Ventana which uses a vortex type of mixing. Good luck! Thanks, Timothy N. Higgins, HT (ASCP), QIHC > Date: Mon, 23 Sep 2013 14:00:57 -0700 (PDT) > From: Mary Benoit > Subject: [Histonet] edge effect on IHC > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > <1379970057.43816.YahooMailNeo@web142505.mail.bf1.yahoo.com> > Content-Type: text/plain; charset=us-ascii > > I have been aware of some "edge effect" on an occasional slide, but lately we noticed the effect is showing up in the area of the slide that is farthest from the labels. We use DAKO Link Autostainer and their pretreatment baths for everything. One case in particular we stained a large melanoma met to colon that was well fixed and processed that stained only the edge, however when we sent block to Neogenomics reference lab, their slide stained evenly over the entire tumor area. We both used a melanoma cocktail. Not sure what is going on. Any suggestions? > Thank you > Mary F Benoit, MT(ASCP) > The Pathology Laboratory > 830 Bayou Pines Drive > Lake Charles, LA 70601 > mbplab@yahoo.com ------------------------------ Message: 16 Date: Wed, 25 Sep 2013 09:58:06 -0500 From: Jan Shivers Subject: Re: [Histonet] edge effect on IHC To: Tim Higgins Cc: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Also make sure that the tissue never dries out on the trimming table before it even gets to formalin. That can also be a source of edge effect. Jan Shivers UMN On Wed, Sep 25, 2013 at 7:37 AM, Tim Higgins wrote: > Hey Mary, > > > > I would suggest observing your > stainer to ensure the proper drop zones are being performed and not just > dropping closest to the label. I used Dako for years and loved them but > you do > have to make sure it dispenses properly from time to time. They use drop > zones > unlike Ventana which uses a vortex type of mixing. > > > > Good luck! > > > > Thanks, > > > > Timothy N. Higgins, HT (ASCP), QIHC > > > > > > > Date: Mon, 23 Sep 2013 14:00:57 -0700 (PDT) > > From: Mary Benoit > > Subject: [Histonet] edge effect on IHC > > To: "histonet@lists.utsouthwestern.edu" > > > > Message-ID: > > <1379970057.43816.YahooMailNeo@web142505.mail.bf1.yahoo.com> > > Content-Type: text/plain; charset=us-ascii > > > > I have been aware of some "edge effect" on an occasional slide, but > lately we noticed the effect is showing up in the area of the slide that is > farthest from the labels. We use DAKO Link Autostainer and their > pretreatment baths for everything. One case in particular we stained a > large melanoma met to colon that was well fixed and processed that stained > only the edge, however when we sent block to Neogenomics reference lab, > their slide stained evenly over the entire tumor area. We both used a > melanoma cocktail. Not sure what is going on. Any suggestions? > > Thank you > > Mary F Benoit, MT(ASCP) > > The Pathology Laboratory > > 830 Bayou Pines Drive > > Lake Charles, LA 70601 > > mbplab@yahoo.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 17 Date: Wed, 25 Sep 2013 08:15:46 -0700 From: Jackie Lair Subject: Re: [Histonet] RE: Peggy Wenks award To: "Tony Henwood (SCHN)" Cc: "Histonet \(histonet@lists.utsouthwestern.edu\)" , "histonet-bounces@lists.utsouthwestern.edu" , Sue Hunter Message-ID: <6B319F96-AAFF-487D-9292-30C873758BEA@gmail.com> Content-Type: text/plain; charset=us-ascii Congrats Peggy!!! :) Jackie Lair Sent from my iPhone On Sep 24, 2013, at 4:09 PM, "Tony Henwood (SCHN)" wrote: > Congratulations, well deserved from down-under > > Regards > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) > Laboratory Manager & Senior Scientist > Tel: 612 9845 3306 > Fax: 612 9845 3318 > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead > Locked Bag 4001, Westmead NSW 2145, AUSTRALIA > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. > Sent: Tuesday, 24 September 2013 10:57 PM > To: 'Sue Hunter'; 'Histonet (histonet@lists.utsouthwestern.edu)'; histonet-bounces@lists.utsouthwestern.edu > Subject: [Histonet] RE: Peggy Wenks award > > Congrats, Peggy. Very well deserved!! > > Joyce Weems > Pathology Manager > 678-843-7376 Phone > 678-843-7831 Fax > joyce.weems@emoryhealthcare.org > > > > www.saintjosephsatlanta.org > 5665 Peachtree Dunwoody Road > Atlanta, GA 30342 > > This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sue Hunter > Sent: Tuesday, September 24, 2013 8:30 AM > To: 'Histonet (histonet@lists.utsouthwestern.edu)'; histonet-bounces@lists.utsouthwestern.edu > Subject: [Histonet] Peggy Wenks award > > For those of you who may not be attending the National Convention or did not hear, we at Beaumont are so proud to announce that Peggy "Amazing" Wenk has won the J.B. McCormick Award. This very prestigious award was presented by Dr. McCormick and was given to Peggy for her outstanding and exceptional service to the NSH. > Please join us in congratulating Peggy on this well deserved recognition and award. > Sue > > Sue Hunter, Supervisor > Advanced Diagnostics > Beaumont Health System > Royal Oak MI > 248-898-5146 > shunter@beaumont.edu > > > > ________________________________ > > This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. > > If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ********************************************************************************* > This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. > > Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. > > This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. > ********************************************************************************* > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 18 Date: Wed, 25 Sep 2013 08:49:03 -0700 (PDT) From: nancy lowen Subject: Re: [Histonet] RE: Submitting Questions To: FeltonNails , 'Joanne Clark' , "histonet@lists.utsouthwestern.edu" , Martha Ward-Pathology Message-ID: <1380124143.69165.YahooMailBasic@web125606.mail.ne1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Me too! -------------------------------------------- On Tue, 9/24/13, Martha Ward-Pathology wrote: Subject: [Histonet] RE: Submitting Questions To: "Nails, Felton" , "'Joanne Clark'" , "histonet@lists.utsouthwestern.edu" Date: Tuesday, September 24, 2013, 12:54 PM I have had the same problem over the past few months... ? Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard ?\? Winston-Salem, NC 27157 p 336.716.2109 ?\? f 336.716.5890 ? mward@wakehealth.edu ? ? ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nails, Felton Sent: Tuesday, September 24, 2013 2:58 PM To: 'Joanne Clark'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Submitting Questions I am in the same situation??? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joanne Clark Sent: Tuesday, September 24, 2013 12:54 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Submitting Questions Can anyone out in histoland explain to me why my submitted questions are not making it through to histonet?? I submitted a question on the 19th of September, and I still have not seen it appear in the group emails I get daily.? I sent the question to the correct address (I checked it to be sure).? When I respond to someones question, I can see my responses, I just can't seem to get an original question submitted.? Can anyone tell me what I am doing wrong? Disclaimer: This electronic message may contain information that is proprietary, confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or? privileged.? If you are not the intended recipient or an? authorized representative of the intended recipient, you? are hereby notified that any review, dissemination, or? copying of this e-mail and its attachments, if any, or? the information contained herein is prohibited.? If you? have received this e-mail in error, please immediately? notify the sender by return e-mail and delete this e-mail? from your computer system.? Thank you. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 118, Issue 43 ***************************************** From flnails <@t> texaschildrens.org Wed Sep 25 13:19:33 2013 From: flnails <@t> texaschildrens.org (Nails, Felton) Date: Wed Sep 25 13:19:48 2013 Subject: [Histonet] 1 Message-ID: <327E034F1892504289B7A17EC71DF9F303BE35@TCFMSG03.ad.texaschildrenshospital.org> test ______________________________________________________________________ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ______________________________________________________________________ From Bruce_Palmatier <@t> vwr.com Wed Sep 25 13:45:10 2013 From: Bruce_Palmatier <@t> vwr.com (Bruce_Palmatier@vwr.com) Date: Wed Sep 25 13:45:20 2013 Subject: [Histonet] AUTO: Bruce Palmatier is out of the office (returning 09/27/2013) Message-ID: I am out of the office until 09/27/2013. I will be out of the office from Sep 25-26th. If you have a question about an order or need assistance placing an order, or if the matter is urgent, please call 877-881-1192 between 8am and 8pm and a VWR Healthcare Service associate will assist you. For quote requests and pricing-related inquiries, I will respond with 24 hours. Thank You, Bruce Palmatier Market Portfolio Manager VWR Healthcare bruce_palmatier@vwr.com mobile: 484.319.5563 fax: 484-881-7307 Customer Service: 877.881.1192 Fax: 484.881.6486 Customer Service email: HEALTHCARESERVICE@VWR.COM Note: This is an automated response to your message "Histonet Digest, Vol 118, Issue 44" sent on 9/25/2013 1:13:07 PM. This is the only notification you will receive while this person is away. From histotalk <@t> yahoo.com Wed Sep 25 14:04:25 2013 From: histotalk <@t> yahoo.com (David Kemler) Date: Wed Sep 25 14:04:31 2013 Subject: [Histonet] RE: Peggy Wenks award In-Reply-To: <1380066882.48974.YahooMailNeo@web121503.mail.ne1.yahoo.com> References: <6D6BD1DE8A5571489398B392A38A71579D29FF73@xmdb04.nch.kids> <1380066882.48974.YahooMailNeo@web121503.mail.ne1.yahoo.com> Message-ID: <1380135865.83890.YahooMailNeo@web121502.mail.ne1.yahoo.com> I interviewed Peggy Sunday morning?at 8 am and she was as bubbly and humble as always. What an amazing lady!?More than likely, her 53 minute interview will be aired on HistoTALK http://www.histotalk.com/ the 2nd Sunday of October, the 14th. A most appropriate time.? ? Yours, Dave ________________________________ From: Tony Henwood (SCHN) To: "'Weems, Joyce K.'" ; 'Sue Hunter' ; "'Histonet (histonet@lists.utsouthwestern.edu)'" ; "histonet-bounces@lists.utsouthwestern.edu" Sent: Tuesday, September 24, 2013 7:09 PM Subject: [Histonet] RE: Peggy Wenks award Congratulations, well deserved from down-under Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Tuesday, 24 September 2013 10:57 PM To: 'Sue Hunter'; 'Histonet (histonet@lists.utsouthwestern.edu)'; histonet-bounces@lists.utsouthwestern.edu Subject: [Histonet] RE: Peggy Wenks award Congrats, Peggy. Very well deserved!! Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org http://www.saintjosephsatlanta.org/ 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s).? It may contain information that is privileged and confidential.? Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sue Hunter Sent: Tuesday, September 24, 2013 8:30 AM To: 'Histonet (histonet@lists.utsouthwestern.edu)'; histonet-bounces@lists.utsouthwestern.edu Subject: [Histonet] Peggy Wenks award For those of you who may not be attending the National Convention or did not hear, we at Beaumont are so proud to announce that? Peggy "Amazing" Wenk has won the J.B. McCormick Award.? This very prestigious award was presented by Dr. McCormick and was given to Peggy for her outstanding and exceptional service to the NSH. Please join us in congratulating Peggy on this well deserved recognition and award. Sue Sue Hunter, Supervisor Advanced Diagnostics Beaumont Health System Royal Oak MI 248-898-5146 shunter@beaumont.edu ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jclark <@t> pcnm.com Wed Sep 25 14:54:57 2013 From: jclark <@t> pcnm.com (Joanne Clark) Date: Wed Sep 25 14:55:14 2013 Subject: [Histonet] Cyclin D1 Message-ID: <0494A7D4E8CC254EA2FB81464982E378B4ABA35C@S10MAILD001N3.SH10.lan> Is anyone running Cyclin D1 on the Leica Bond? I have their ready to use antibody, but it hasn't been worked up on the Bond and my first try has left me with more background than I would like. Does anyone have it worked up with a protocol they would like to share? Joanne Clark, HT(ASCP)CM Director of Histology Pathology Consultants of New Mexico Disclaimer: This electronic message may contain information that is proprietary, confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From jclark <@t> pcnm.com Wed Sep 25 15:00:36 2013 From: jclark <@t> pcnm.com (Joanne Clark) Date: Wed Sep 25 15:02:00 2013 Subject: [Histonet] RE: Submitting Questions In-Reply-To: <002601ceba0b$218f7760$64ae6620$@caplab.org> References: <1380124143.69165.YahooMailBasic@web125606.mail.ne1.yahoo.com> <327E034F1892504289B7A17EC71DF9F303BBC6@TCFMSG03.ad.texaschildrenshospital.org> <002601ceba0b$218f7760$64ae6620$@caplab.org> Message-ID: <0494A7D4E8CC254EA2FB81464982E378B4ABA388@S10MAILD001N3.SH10.lan> I bet that's it. I re-submitted my question and took out all the logo's and fancy stuff after my name. Hopefully it will now appear. -----Original Message----- From: Douglas Porter [mailto:doug.porter@caplab.org] Sent: Wednesday, September 25, 2013 10:20 AM To: 'Nails, Felton'; 'nancy lowen'; Joanne Clark; histonet@lists.utsouthwestern.edu; 'Martha Ward-Pathology' Subject: RE: [Histonet] RE: Submitting Questions I have .jpg files in my signature for initial emails but not in my reply emails. I sent a test message yesterday and got the following reply from the server: Your mail to 'Histonet' with the subject Test Message! Is being held until the list moderator can review it for approval. The reason it is being held: Message body is too big: 119656 bytes with a limit of 60 KB Either the message will get posted to the list, or you will receive notification of the moderator's decision. If you would like to cancel this posting, please visit the following URL: http://lists.utsouthwestern.edu/mailman/confirm/histonet/9abe483614aeb7cc29e e5aa890b57bc164701b31 I suspect the .jpg files are what is causing the problem as the message was only "Test Message!" I will strip out the .jpg files and try again. Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.porter@caplab.org www.caplab.org ? ? The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, copying, forwarding or capture of this communication is strictly prohibited. If you have received this communication in error, please notify me immediately by return e-mail and delete this and all copies. Thank-you. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nails, Felton Sent: Wednesday, September 25, 2013 12:05 PM To: 'nancy lowen'; 'Joanne Clark'; histonet@lists.utsouthwestern.edu; Martha Ward-Pathology Subject: RE: [Histonet] RE: Submitting Questions I have even tried copying the following link (histonet@lists.utsouthwestern.edu) and pasting it and I'm still unable to make a post. -----Original Message----- From: nancy lowen [mailto:claycal44@yahoo.com] Sent: Wednesday, September 25, 2013 10:49 AM To: Nails, Felton; 'Joanne Clark'; histonet@lists.utsouthwestern.edu; Martha Ward-Pathology Subject: Re: [Histonet] RE: Submitting Questions Me too! -------------------------------------------- On Tue, 9/24/13, Martha Ward-Pathology wrote: Subject: [Histonet] RE: Submitting Questions To: "Nails, Felton" , "'Joanne Clark'" , "histonet@lists.utsouthwestern.edu" Date: Tuesday, September 24, 2013, 12:54 PM I have had the same problem over the past few months... ? Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard ?\? Winston-Salem, NC 27157 p 336.716.2109 ?\? f 336.716.5890 mward@wakehealth.edu ? ? ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nails, Felton Sent: Tuesday, September 24, 2013 2:58 PM To: 'Joanne Clark'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Submitting Questions I am in the same situation??? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joanne Clark Sent: Tuesday, September 24, 2013 12:54 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Submitting Questions Can anyone out in histoland explain to me why my submitted questions are not making it through to histonet?? I submitted a question on the 19th of September, and I still have not seen it appear in the group emails I get daily.? I sent the question to the correct address (I checked it to be sure).? When I respond to someones question, I can see my responses, I just can't seem to get an original question submitted.? Can anyone tell me what I am doing wrong? Disclaimer: This electronic message may contain information that is proprietary, confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or? privileged. If you are not the intended recipient or an? authorized representative of the intended recipient, you? are hereby notified that any review, dissemination, or? copying of this e-mail and its attachments, if any, or the information contained herein is prohibited.? If you? have received this e-mail in error, please immediately? notify the sender by return e-mail and delete this e-mail? from your computer system.? Thank you. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Disclaimer: This electronic message may contain information that is proprietary, confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From PAMarcum <@t> uams.edu Wed Sep 25 15:14:46 2013 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Wed Sep 25 15:14:55 2013 Subject: [Histonet] RE: Submitting Questions In-Reply-To: <0494A7D4E8CC254EA2FB81464982E378B4ABA388@S10MAILD001N3.SH10.lan> References: <1380124143.69165.YahooMailBasic@web125606.mail.ne1.yahoo.com> <327E034F1892504289B7A17EC71DF9F303BBC6@TCFMSG03.ad.texaschildrenshospital.org> <002601ceba0b$218f7760$64ae6620$@caplab.org> <0494A7D4E8CC254EA2FB81464982E378B4ABA388@S10MAILD001N3.SH10.lan> Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA32D315AB22@Mail2Node2.ad.uams.edu> I have also piggybacked on another HistoNet e-mail when nothing else worked. Always with an apology to the original submitter. Pam Marcum -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joanne Clark Sent: Wednesday, September 25, 2013 3:01 PM To: Douglas Porter; 'Nails, Felton'; 'nancy lowen'; histonet@lists.utsouthwestern.edu; 'Martha Ward-Pathology' Subject: RE: [Histonet] RE: Submitting Questions I bet that's it. I re-submitted my question and took out all the logo's and fancy stuff after my name. Hopefully it will now appear. -----Original Message----- From: Douglas Porter [mailto:doug.porter@caplab.org] Sent: Wednesday, September 25, 2013 10:20 AM To: 'Nails, Felton'; 'nancy lowen'; Joanne Clark; histonet@lists.utsouthwestern.edu; 'Martha Ward-Pathology' Subject: RE: [Histonet] RE: Submitting Questions I have .jpg files in my signature for initial emails but not in my reply emails. I sent a test message yesterday and got the following reply from the server: Your mail to 'Histonet' with the subject Test Message! Is being held until the list moderator can review it for approval. The reason it is being held: Message body is too big: 119656 bytes with a limit of 60 KB Either the message will get posted to the list, or you will receive notification of the moderator's decision. If you would like to cancel this posting, please visit the following URL: http://lists.utsouthwestern.edu/mailman/confirm/histonet/9abe483614aeb7cc29e e5aa890b57bc164701b31 I suspect the .jpg files are what is causing the problem as the message was only "Test Message!" I will strip out the .jpg files and try again. Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.porter@caplab.org www.caplab.org ? ? The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, copying, forwarding or capture of this communication is strictly prohibited. If you have received this communication in error, please notify me immediately by return e-mail and delete this and all copies. Thank-you. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nails, Felton Sent: Wednesday, September 25, 2013 12:05 PM To: 'nancy lowen'; 'Joanne Clark'; histonet@lists.utsouthwestern.edu; Martha Ward-Pathology Subject: RE: [Histonet] RE: Submitting Questions I have even tried copying the following link (histonet@lists.utsouthwestern.edu) and pasting it and I'm still unable to make a post. -----Original Message----- From: nancy lowen [mailto:claycal44@yahoo.com] Sent: Wednesday, September 25, 2013 10:49 AM To: Nails, Felton; 'Joanne Clark'; histonet@lists.utsouthwestern.edu; Martha Ward-Pathology Subject: Re: [Histonet] RE: Submitting Questions Me too! -------------------------------------------- On Tue, 9/24/13, Martha Ward-Pathology wrote: Subject: [Histonet] RE: Submitting Questions To: "Nails, Felton" , "'Joanne Clark'" , "histonet@lists.utsouthwestern.edu" Date: Tuesday, September 24, 2013, 12:54 PM I have had the same problem over the past few months... ? Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard ?\? Winston-Salem, NC 27157 p 336.716.2109 ?\? f 336.716.5890 mward@wakehealth.edu ? ? ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nails, Felton Sent: Tuesday, September 24, 2013 2:58 PM To: 'Joanne Clark'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Submitting Questions I am in the same situation??? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joanne Clark Sent: Tuesday, September 24, 2013 12:54 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Submitting Questions Can anyone out in histoland explain to me why my submitted questions are not making it through to histonet?? I submitted a question on the 19th of September, and I still have not seen it appear in the group emails I get daily.? I sent the question to the correct address (I checked it to be sure).? When I respond to someones question, I can see my responses, I just can't seem to get an original question submitted.? Can anyone tell me what I am doing wrong? Disclaimer: This electronic message may contain information that is proprietary, confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or? privileged. If you are not the intended recipient or an? authorized representative of the intended recipient, you? are hereby notified that any review, dissemination, or? copying of this e-mail and its attachments, if any, or the information contained herein is prohibited.? If you? have received this e-mail in error, please immediately? notify the sender by return e-mail and delete this e-mail? from your computer system.? Thank you. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Disclaimer: This electronic message may contain information that is proprietary, confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From Donna.Millard <@t> prlnet.com Wed Sep 25 15:27:59 2013 From: Donna.Millard <@t> prlnet.com (Donna Millard) Date: Wed Sep 25 15:28:06 2013 Subject: [Histonet] Cytology processing staffing Message-ID: <64D11CD058311340A1966C234C6B0B890FA311FA@PRLEXCH02.prlnet.com> For those of you also over cytology, how do you staff for cytology processors? I can't find a benchmark for # of PAPs and nongyns / processor. Our processors accession, process, answer phone calls, call on requisition discrepancies, send specimens for molecular testing... Feedback is appreciated. Donna Millard Director of Anatomic Pathology Physicians Reference Laboratory, LLC 7800 W. 110th Street,Overland Park, KS 66210 Direct: 913-339-0485 Fax: 913-319-4156 CONFIDENTIALITY NOTICE This message and any included attachments are from Physicians Reference Laboratory, LLC and are intended only for the addressee.The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call PRL's corporate offices in Overland Park, Kansas, U.S.A at (913)338-4070 From mucram11 <@t> comcast.net Wed Sep 25 15:29:27 2013 From: mucram11 <@t> comcast.net (Pam Marcum) Date: Wed Sep 25 15:29:35 2013 Subject: [Histonet] Question for Our IHC Area Message-ID: <1679103112.1187928.1380140967070.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Is anyone having an issue with Ventana's Pan cytokeratin?? Please answer offline. ? Pam Marcum From mhanna <@t> histosearch.com Wed Sep 25 17:20:11 2013 From: mhanna <@t> histosearch.com (mhanna@histosearch.com) Date: Wed Sep 25 17:20:22 2013 Subject: [Histonet] RE: Submitting Questions In-Reply-To: <41D3A1AF6FEF0643BDC89E0516A6EA32D315AB22@Mail2Node2.ad.uams.edu> References: <1380124143.69165.YahooMailBasic@web125606.mail.ne1.yahoo.com> <327E034F1892504289B7A17EC71DF9F303BBC6@TCFMSG03.ad.texaschildrenshospital.org> <002601ceba0b$218f7760$64ae6620$@caplab.org> <0494A7D4E8CC254EA2FB81464982E378B4ABA388@S10MAILD001N3.SH10.lan> <41D3A1AF6FEF0643BDC89E0516A6EA32D315AB22@Mail2Node2.ad.uams.edu> Message-ID: <5243619B.8000906@histosearch.com> All emails with any attachments will be blocked by the listserver. This includes jpg, gif and png image files, as well as anything else attached to your email. This is because it is possible to code viruses and other malware in attachments and the server is trying to protect us. Here is the information from the initial email we all received when we subscribed to Histonet (Subject: Welcome to the "Histonet" mailing list) : "IMPORTANT INFORMATION: Please do not send images or any other attachments with your message. The server will block all attachments to protect the list against viruses." The server actually blocks and deletes the entire email with the attachment. It is right before the section explaining how to unsubscribe... Best Regards, Marvin Hanna On 9/25/2013 4:14 PM, Marcum, Pamela A wrote: > I have also piggybacked on another HistoNet e-mail when nothing else worked. Always with an apology to the original submitter. Pam Marcum > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joanne Clark > Sent: Wednesday, September 25, 2013 3:01 PM > To: Douglas Porter; 'Nails, Felton'; 'nancy lowen'; histonet@lists.utsouthwestern.edu; 'Martha Ward-Pathology' > Subject: RE: [Histonet] RE: Submitting Questions > > I bet that's it. I re-submitted my question and took out all the logo's and fancy stuff after my name. Hopefully it will now appear. > > -----Original Message----- > From: Douglas Porter [mailto:doug.porter@caplab.org] > Sent: Wednesday, September 25, 2013 10:20 AM > To: 'Nails, Felton'; 'nancy lowen'; Joanne Clark; histonet@lists.utsouthwestern.edu; 'Martha Ward-Pathology' > Subject: RE: [Histonet] RE: Submitting Questions > > I have .jpg files in my signature for initial emails but not in my reply emails. I sent a test message yesterday and got the following reply from the server: > > Your mail to 'Histonet' with the subject > > Test Message! > > Is being held until the list moderator can review it for approval. > > The reason it is being held: > > Message body is too big: 119656 bytes with a limit of 60 KB > > Either the message will get posted to the list, or you will receive notification of the moderator's decision. If you would like to cancel this posting, please visit the following URL: > > > http://lists.utsouthwestern.edu/mailman/confirm/histonet/9abe483614aeb7cc29e > e5aa890b57bc164701b31 > > I suspect the .jpg files are what is causing the problem as the message was only "Test Message!" I will strip out the .jpg files and try again. > > > Douglas A. Porter, HT (ASCP) > Grossing Technician > IT Coordinator > Cancer Registrar > > CAP-Lab, PLC > 2508 South Cedar Street > Lansing, MI 48910-3138 > > 517-372-5520 (phone) > 517-372-5540 (fax) > > doug.porter@caplab.org > > www.caplab.org > > > The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, copying, forwarding or capture of this communication is strictly prohibited. If you have received this communication in error, please notify me immediately by return e-mail and delete this and all copies. Thank-you. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nails, Felton > Sent: Wednesday, September 25, 2013 12:05 PM > To: 'nancy lowen'; 'Joanne Clark'; histonet@lists.utsouthwestern.edu; Martha Ward-Pathology > Subject: RE: [Histonet] RE: Submitting Questions > > I have even tried copying the following link > (histonet@lists.utsouthwestern.edu) and pasting it and I'm still unable to make a post. > > -----Original Message----- > From: nancy lowen [mailto:claycal44@yahoo.com] > Sent: Wednesday, September 25, 2013 10:49 AM > To: Nails, Felton; 'Joanne Clark'; histonet@lists.utsouthwestern.edu; Martha Ward-Pathology > Subject: Re: [Histonet] RE: Submitting Questions > > Me too! > -------------------------------------------- > On Tue, 9/24/13, Martha Ward-Pathology wrote: > > Subject: [Histonet] RE: Submitting Questions > To: "Nails, Felton" , "'Joanne Clark'" > , "histonet@lists.utsouthwestern.edu" > > Date: Tuesday, September 24, 2013, 12:54 PM > > I have had the same problem over the > past few months... > > Martha Ward, MT (ASCP) QIHC > Manager > > Molecular Diagnostics Lab > Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f > 336.716.5890 mward@wakehealth.edu > > > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] > On Behalf Of Nails, Felton > Sent: Tuesday, September 24, 2013 2:58 PM > To: 'Joanne Clark'; histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: Submitting Questions > > I am in the same situation??? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] > On Behalf Of Joanne Clark > Sent: Tuesday, September 24, 2013 12:54 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Submitting Questions > > Can anyone out in histoland explain to me why my submitted questions are not making it through to histonet? I submitted a question on the 19th of September, and I still have not seen it appear in the group emails I get daily. I sent the question to the correct address (I checked it to be sure). When I respond to someones question, I can see my responses, I just can't seem to get an original question submitted. Can anyone tell me what I am doing wrong? > > > > > Disclaimer: This electronic message may contain information that is proprietary, confidential, or legally privileged or protected. It is intended only for the use of the > individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ______________________________________________________________________ > CONFIDENTIALITY NOTICE: > The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. > ______________________________________________________________________ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ______________________________________________________________________ > CONFIDENTIALITY NOTICE: > The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. > ______________________________________________________________________ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > Disclaimer: This electronic message may contain information that is proprietary, confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ---------------------------------------------------------------------- > Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From kate.lillard <@t> yahoo.com Thu Sep 26 02:50:19 2013 From: kate.lillard <@t> yahoo.com (Kate Lillard) Date: Thu Sep 26 02:57:59 2013 Subject: [Histonet] O Message-ID: Oaloaka Sent from my iPhone From DianaRip1 <@t> aol.com Thu Sep 26 07:15:53 2013 From: DianaRip1 <@t> aol.com (DianaRip1@aol.com) Date: Thu Sep 26 07:15:58 2013 Subject: [Histonet] Good Old Days Message-ID: <804e9.651971a1.3f757f79@aol.com> Ahhh yes. I must truly be old. I remember all of these things with a smile. Diana Ripley From flnails <@t> texaschildrens.org Thu Sep 26 12:02:39 2013 From: flnails <@t> texaschildrens.org (Nails, Felton) Date: Thu Sep 26 12:03:24 2013 Subject: [Histonet] test Message-ID: <327E034F1892504289B7A17EC71DF9F303C3B5@TCFMSG03.ad.texaschildrenshospital.org> 9-26-2013 ______________________________________________________________________ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ______________________________________________________________________ From egray <@t> hsc.wvu.edu Thu Sep 26 13:13:13 2013 From: egray <@t> hsc.wvu.edu (Gray, Ed) Date: Thu Sep 26 13:13:24 2013 Subject: [Histonet] RE: Cytology processing staffing Message-ID: <0BBA94171603A6468FFBA03A45A029FC0444C92FB7@MSEXCL.hscresex.local> We have two people processing approximately 8000 PAPs, 2200 Non-Gyns and 600 FNAs. In addition to the duties you mentioned our folks also pickup specimens twice a day in various locations (roughly 30 min per run); file slides and paperwork for cytology; assist with filing surgical pathology; merge patients in our APLIS; and of course the ever-present "other duties as assigned." Not a great benchmark but maybe it will help. Ed Gray | Pathology IT Analyst | Phone: 304-293-2945 | Fax 304-293-1627 | WVU Healthcare l egray@wvuhealthcare.com Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ---------------------------------------------------------------------- Message: 1 Date: Wed, 25 Sep 2013 20:27:59 +0000 From: Donna Millard > Subject: [Histonet] Cytology processing staffing To: "histonet@lists.utsouthwestern.edu" > Message-ID: <64D11CD058311340A1966C234C6B0B890FA311FA@PRLEXCH02.prlnet.com> Content-Type: text/plain; charset="us-ascii" For those of you also over cytology, how do you staff for cytology processors? I can't find a benchmark for # of PAPs and nongyns / processor. Our processors accession, process, answer phone calls, call on requisition discrepancies, send specimens for molecular testing... Feedback is appreciated. Donna Millard Director of Anatomic Pathology Physicians Reference Laboratory, LLC 7800 W. 110th Street,Overland Park, KS 66210 Direct: 913-339-0485 Fax: 913-319-4156 CONFIDENTIALITY NOTICE This message and any included attachments are from Physicians Reference Laboratory, LLC and are intended only for the addressee.The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call PRL's corporate offices in Overland Park, Kansas, U.S.A at (913)338-4070 ------------------------------ From amber.mckenzie <@t> gastrodocs.net Thu Sep 26 13:27:52 2013 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Thu Sep 26 13:27:57 2013 Subject: [Histonet] Lab Chairs In-Reply-To: <0BBA94171603A6468FFBA03A45A029FC0444C92FB7@MSEXCL.hscresex.local> References: <0BBA94171603A6468FFBA03A45A029FC0444C92FB7@MSEXCL.hscresex.local> Message-ID: <5A33C952BB67F4468AF1F36D739212BCBD6EA58B@JERRY.Gia.com> We are looking at buying new chairs for our lab...any suggestions? From melissa <@t> alliedsearchpartners.com Thu Sep 26 14:54:07 2013 From: melissa <@t> alliedsearchpartners.com (Melissa Phelan) Date: Thu Sep 26 14:54:28 2013 Subject: [Histonet] Histotech Needed for Vacation Coverage-Aurora, CO (October) Message-ID: Hello, I am looking for a Histotech who has recent experience in a dermatology lab to cover for 3 days in October within a lab in Aurora, CO area. See below for details and please contact me with your resume with salary requirements if you can be available. Allied Search Partners, the leading provider of Laboratory professionals in the US is looking for a tech who can cover for a permanent Histotech's vacation. Requirements: ? Must live in the Aurora, CO area ? Must have recent experience with Dermatological Specimens ? ASCP preferred ? Associates or Bachelor?s Degree preferred Schedule: 7am-4pm When: Oct. 16th, 17th, 18th (Wednesday, Thursday, and Friday) If interested, please send resume to Melissa@alliedsearchpartners.com or fax to 888-388-7572. Please also send salary requirements. To view a complete list of Allied Search Partners current openings go to: http://www.alliedsearchpartners.com/careers.php -- Melissa Phelan President, Laboratory Staffing Allied Search Partners www.linkedin.com/in/melissaphelan/ http://www.alliedsearchpartners.com T: 888.388.7571 ext. 102 F: 888.388.7572 *If you wish to no longer receive emails from Allied Search Partners please respond to this email message with "remove." This email including its attachments is intended only for the confidential use of the individual to whom it is addressed. If you are not the intended recipient, any use, dissemination, distribution or copying of this message or its attachments is prohibited. If you have received this message in error, please notify us immediately, and delete this message and its attachments permanently from your system. From lowenjeff <@t> hotmail.com Thu Sep 26 15:25:42 2013 From: lowenjeff <@t> hotmail.com (jeff lowen) Date: Thu Sep 26 15:25:51 2013 Subject: [Histonet] Lab Chairs In-Reply-To: <5A33C952BB67F4468AF1F36D739212BCBD6EA58B@JERRY.Gia.com> References: <0BBA94171603A6468FFBA03A45A029FC0444C92FB7@MSEXCL.hscresex.local>, <5A33C952BB67F4468AF1F36D739212BCBD6EA58B@JERRY.Gia.com> Message-ID: bean bags > From: amber.mckenzie@gastrodocs.net > To: histonet@lists.utsouthwestern.edu > Date: Thu, 26 Sep 2013 18:27:52 +0000 > Subject: [Histonet] Lab Chairs > > We are looking at buying new chairs for our lab...any suggestions? > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amber.mckenzie <@t> gastrodocs.net Thu Sep 26 15:55:47 2013 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Thu Sep 26 15:56:00 2013 Subject: [Histonet] Height detail In-Reply-To: References: Message-ID: <5A33C952BB67F4468AF1F36D739212BCBD6EA704@JERRY.Gia.com> What's the height limit you can stack blocks/slides according to CAP? From chapcl <@t> yahoo.com Thu Sep 26 16:01:17 2013 From: chapcl <@t> yahoo.com (Will Chappell) Date: Thu Sep 26 16:01:30 2013 Subject: [Histonet] Height detail In-Reply-To: <5A33C952BB67F4468AF1F36D739212BCBD6EA704@JERRY.Gia.com> References: <5A33C952BB67F4468AF1F36D739212BCBD6EA704@JERRY.Gia.com> Message-ID: <5DC05C96-5D6D-4911-927E-990A492BDBC0@yahoo.com> None according to cap. Maybe something from OSHA. Must maintain a certain distance from ceiling (8-12 inches I think) and must be up off floor (if stored in lab). They must be safe, however that is subjective. Will Chappell, HTL(ASCP) QIHC And available. Sent from my iPhone > On Sep 26, 2013, at 1:55 PM, Amber McKenzie wrote: > > What's the height limit you can stack blocks/slides according to CAP? > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjr6 <@t> psu.edu Fri Sep 27 06:20:57 2013 From: rjr6 <@t> psu.edu (Roberta Horner) Date: Fri Sep 27 06:23:17 2013 Subject: [Histonet] Lab Chairs In-Reply-To: References: <0BBA94171603A6468FFBA03A45A029FC0444C92FB7@MSEXCL.hscresex.local>, <5A33C952BB67F4468AF1F36D739212BCBD6EA58B@JERRY.Gia.com>, Message-ID: I have 2 saddle chairs and I really like them. You have to sit straight on them, no slouching. I have less back aches since I got them. Roberta Horner HT/HTL Penn State University Animal Diagnostic Lab From trathborne <@t> somerset-healthcare.com Fri Sep 27 06:36:05 2013 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Fri Sep 27 06:36:58 2013 Subject: [Histonet] Lab Chairs In-Reply-To: References: <0BBA94171603A6468FFBA03A45A029FC0444C92FB7@MSEXCL.hscresex.local>, <5A33C952BB67F4468AF1F36D739212BCBD6EA58B@JERRY.Gia.com>, Message-ID: <3AD061FE740D464FAC7BF6B5CFB75707A8A6E24E@smcmail02.somerset-healthcare.com> Do you have a link to where we could see them? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Roberta Horner Sent: Friday, September 27, 2013 7:21 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Lab Chairs I have 2 saddle chairs and I really like them. You have to sit straight on them, no slouching. I have less back aches since I got them. Roberta Horner HT/HTL Penn State University Animal Diagnostic Lab _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From arguellod <@t> ihn.org Fri Sep 27 08:36:32 2013 From: arguellod <@t> ihn.org (Arguello, Daniel) Date: Fri Sep 27 08:37:24 2013 Subject: [Histonet] Shredding biopsies Message-ID: <46FD76128C5055478050297AEAC97E170C89F343@EXCHANGE-1.corporate.lan> Dear Histonetters, I need your help troubleshooting an issue we've had with our colon biopsies since we switched from Safefix (alcohol fixative) to 10% formalin. The complaint I get from the pathologists is that the tissue on these biopsies seems to be shredding, specially on the edge of the tissue. I am wondering if it is a over-fixation issue. We use Soft block (http://www.polysciences.com/sitedata/poly/assets/datasheets/718.pdf), but it seems like it doesn't help much. I would be very grateful if you can share your processing schedule for biopsies or any other tip that may help us resolve this issue. Thanks in advance. Sincerely, Daniel Arg?ello, BS, CT(ASCP)CM Anatomic Pathology Coordinator Inspira Medical Center Woodbury Telephone (856) 853-2030 Telephone (856) 845-0100 Ext 2808 Fax (856) 853-2183 arguellod@ihn.org WEB: http://www.inspirahealthnetwork.org/ From joewalker <@t> rrmc.org Fri Sep 27 08:48:00 2013 From: joewalker <@t> rrmc.org (Joe W. Walker, Jr.) Date: Fri Sep 27 08:48:06 2013 Subject: [Histonet] Urine Cytology In-Reply-To: <1379345369.7648.YahooMailNeo@web161205.mail.bf1.yahoo.com> References: <1379345369.7648.YahooMailNeo@web161205.mail.bf1.yahoo.com> Message-ID: <3C2378778400AD448ADA6FD6BDB7CCCC180DCD2B@RRMBX03.rrmc.local> Unfortunately, It would depend on the fixative used and the ratio of fixative to specimen. Do you happen to know this information? Joe W. Walker, Jr. MS, SCT(ASCP)CM Anatomical Pathology Manager Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P: 802.747.1790 F: 802.747.6525 Email joewalker@rrmc.org www.rrmc.org Our Vision: To be the Best Community Healthcare System in New England Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet Recognition? and the Governor's Award for Performance Excellence -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Debbie Granato Sent: Monday, September 16, 2013 11:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Urine Cytology Good Morning! Can anyone please tell me the longest that a fixed urine cytology can stay in the refrigerator before processing. I appreciate your help and input! Debbie Granato HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. Thank You From joewalker <@t> rrmc.org Fri Sep 27 08:51:26 2013 From: joewalker <@t> rrmc.org (Joe W. Walker, Jr.) Date: Fri Sep 27 08:51:34 2013 Subject: [Histonet] RE: Cytology processing staffing In-Reply-To: <0BBA94171603A6468FFBA03A45A029FC0444C92FB7@MSEXCL.hscresex.local> References: <0BBA94171603A6468FFBA03A45A029FC0444C92FB7@MSEXCL.hscresex.local> Message-ID: <3C2378778400AD448ADA6FD6BDB7CCCC180DCD71@RRMBX03.rrmc.local> Unfortunately, I am unaware of an official benchmark. We have a similar volume to Ed's but the job is accomplished by 1 person. We do receive the majority of our orders for Pap's, Non's and FNA's electronically so her paperwork duties are minimum. Prior to when we made a transition to electronic orders and interfaced orders we had 1.5 FTE accomplishing the task. I hope this helps, Joe W. Walker, Jr. MS, SCT(ASCP)CM Anatomical Pathology Manager Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P: 802.747.1790 F: 802.747.6525 Email joewalker@rrmc.org www.rrmc.org Our Vision: To be the Best Community Healthcare System in New England Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet Recognition? and the Governor's Award for Performance Excellence -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gray, Ed Sent: Thursday, September 26, 2013 2:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Cytology processing staffing We have two people processing approximately 8000 PAPs, 2200 Non-Gyns and 600 FNAs. In addition to the duties you mentioned our folks also pickup specimens twice a day in various locations (roughly 30 min per run); file slides and paperwork for cytology; assist with filing surgical pathology; merge patients in our APLIS; and of course the ever-present "other duties as assigned." Not a great benchmark but maybe it will help. Ed Gray | Pathology IT Analyst | Phone: 304-293-2945 | Fax 304-293-1627 | WVU Healthcare l egray@wvuhealthcare.com Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ---------------------------------------------------------------------- Message: 1 Date: Wed, 25 Sep 2013 20:27:59 +0000 From: Donna Millard > Subject: [Histonet] Cytology processing staffing To: "histonet@lists.utsouthwestern.edu" > Message-ID: <64D11CD058311340A1966C234C6B0B890FA311FA@PRLEXCH02.prlnet.com> Content-Type: text/plain; charset="us-ascii" For those of you also over cytology, how do you staff for cytology processors? I can't find a benchmark for # of PAPs and nongyns / processor. Our processors accession, process, answer phone calls, call on requisition discrepancies, send specimens for molecular testing... Feedback is appreciated. Donna Millard Director of Anatomic Pathology Physicians Reference Laboratory, LLC 7800 W. 110th Street,Overland Park, KS 66210 Direct: 913-339-0485 Fax: 913-319-4156 CONFIDENTIALITY NOTICE This message and any included attachments are from Physicians Reference Laboratory, LLC and are intended only for the addressee.The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call PRL's corporate offices in Overland Park, Kansas, U.S.A at (913)338-4070 ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. Thank You From one_angel_secret <@t> yahoo.com Fri Sep 27 12:45:38 2013 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Fri Sep 27 12:45:51 2013 Subject: [Histonet] Height detail In-Reply-To: <5A33C952BB67F4468AF1F36D739212BCBD6EA704@JERRY.Gia.com> References: <5A33C952BB67F4468AF1F36D739212BCBD6EA704@JERRY.Gia.com> Message-ID: <95F6E043-FD0B-46B1-9A77-A20B7A72F1EF@yahoo.com> You can't stack anything higher than 18 inches from the ceiling. This is the only stack law I'm personally aware of. Sent from my iPhone On Sep 26, 2013, at 4:55 PM, Amber McKenzie wrote: > What's the height limit you can stack blocks/slides according to CAP? > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From billodonnell <@t> catholichealth.net Fri Sep 27 13:29:52 2013 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Fri Sep 27 13:30:10 2013 Subject: [Histonet] Height detail In-Reply-To: <95F6E043-FD0B-46B1-9A77-A20B7A72F1EF@yahoo.com> References: <5A33C952BB67F4468AF1F36D739212BCBD6EA704@JERRY.Gia.com> <95F6E043-FD0B-46B1-9A77-A20B7A72F1EF@yahoo.com> Message-ID: CAP has no height limit. Your local fire marshal or safety department may well have a ceiling distance minimum. Most places I have been have the 18" distance from ceiling so that fire sprinklers are not blocked - but please check with your safety dept. as yours may well be different. William (Bill) O'Donnell, HT (ASCP) QIHC Senior Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 SERENITY is not freedom from the storm, but peace amid the storm. Cultivate it in PRAYER! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Donadio Sent: Friday, September 27, 2013 12:46 PM To: Amber McKenzie Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Height detail You can't stack anything higher than 18 inches from the ceiling. This is the only stack law I'm personally aware of. Sent from my iPhone On Sep 26, 2013, at 4:55 PM, Amber McKenzie wrote: > What's the height limit you can stack blocks/slides according to CAP? > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. From DFRANKHOUSER <@t> lewistownhospital.org Fri Sep 27 13:58:03 2013 From: DFRANKHOUSER <@t> lewistownhospital.org (Frankhouser, Deena) Date: Fri Sep 27 13:58:19 2013 Subject: [Histonet] continuing ed Message-ID: Hi I am interested in free CME credits in Histology. Please let me know if any members know of any free education courses. Thank you Deena Frankhouser dfrankhouser@lewistownhospital.org From liz <@t> premierlab.com Fri Sep 27 14:04:45 2013 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Fri Sep 27 14:04:57 2013 Subject: [Histonet] RE: continuing ed In-Reply-To: References: Message-ID: <14E2C6176416974295479C64A11CB9AE019BB56FE1DD@SBS2K8.premierlab.local> Both Sakura and Leica have free webinars that will give you CEU credits. I think you can sign up for them on line. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Laboratory Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 Work (303) 682-3949 Fax (303) 682-9060 Cell (303) 881-0763 liz@premierlab.com www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Frankhouser, Deena Sent: Friday, September 27, 2013 12:58 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] continuing ed Hi I am interested in free CME credits in Histology. Please let me know if any members know of any free education courses. Thank you Deena Frankhouser dfrankhouser@lewistownhospital.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mthomas <@t> littonlab.com Fri Sep 27 14:10:06 2013 From: mthomas <@t> littonlab.com (Marla Thomas) Date: Fri Sep 27 14:10:22 2013 Subject: [Histonet] Height detail In-Reply-To: References: <5A33C952BB67F4468AF1F36D739212BCBD6EA704@JERRY.Gia.com> <95F6E043-FD0B-46B1-9A77-A20B7A72F1EF@yahoo.com> Message-ID: <5bae6ce3beab4ae38e21e5622fae4400@BLUPR07MB178.namprd07.prod.outlook.com> OSHA requires 18" distance from the ceiling. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Friday, September 27, 2013 1:30 PM To: Kim Donadio; Amber McKenzie Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Height detail CAP has no height limit. Your local fire marshal or safety department may well have a ceiling distance minimum. Most places I have been have the 18" distance from ceiling so that fire sprinklers are not blocked - but please check with your safety dept. as yours may well be different. William (Bill) O'Donnell, HT (ASCP) QIHC Senior Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 SERENITY is not freedom from the storm, but peace amid the storm. Cultivate it in PRAYER! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Donadio Sent: Friday, September 27, 2013 12:46 PM To: Amber McKenzie Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Height detail You can't stack anything higher than 18 inches from the ceiling. This is the only stack law I'm personally aware of. Sent from my iPhone On Sep 26, 2013, at 4:55 PM, Amber McKenzie wrote: > What's the height limit you can stack blocks/slides according to CAP? > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Lisa.Freeman <@t> fda.hhs.gov Fri Sep 27 14:22:25 2013 From: Lisa.Freeman <@t> fda.hhs.gov (Freeman, Lisa*) Date: Fri Sep 27 14:22:36 2013 Subject: [Histonet] Fatty Mammary Gland Processing Message-ID: <0F928833A4CE744793039B781368BED01276FEA8@FDSWP3313.fda.gov> I would greatly appreciate any input you have on processing fatty rodent mammary glands. Our current processing protocol for mammary gland/large animal is 16 hours long. 1 hour X 8 ETOH (graded from 70% to 100%) 1 hour 20 minutes X 3 Xylene 1 hour 20 minutes X 3 paraffin The rodents are very large and are not processing adequately. We have reprocessed the samples with success. Thank you, Lisa Freeman, HT Histology Supervisor Toxicologic Pathology Associates National Center for Toxicological Research 3900 NCTR RD Jefferson, AR 72079 Phone: 870-543-7234 E-mail: Lisa.Freeman@fda.hhs.gov From Benjamin.Dubansky <@t> unt.edu Fri Sep 27 15:51:06 2013 From: Benjamin.Dubansky <@t> unt.edu (Dubansky, Benjamin) Date: Fri Sep 27 15:51:25 2013 Subject: [Histonet] Microwave Processors Message-ID: <22A8598E6721584AA9B6BFC09DAED911FA4CE0@GABMBx03.ad.unt.edu> Can some of you weigh in on how you feel about microwave histoprocessors? I am terrified that my samples will end up like raisins. "They" swear that these milestone processors are just as good as any other method, in terms of quality. I'ts all I have right now and it is very tempting to use it, although I am considering doing things the old fashioned way. And I am talking about pre-processor old fashioned way. I am that scared. Ben From Timothy.Morken <@t> ucsfmedctr.org Fri Sep 27 17:12:06 2013 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Fri Sep 27 17:14:29 2013 Subject: [Histonet] RE: Microwave Processors In-Reply-To: <22A8598E6721584AA9B6BFC09DAED911FA4CE0@GABMBx03.ad.unt.edu> References: <22A8598E6721584AA9B6BFC09DAED911FA4CE0@GABMBx03.ad.unt.edu> Message-ID: <761E2B5697F795489C8710BCC72141FF09B9E2@ex07.net.ucsf.edu> Ben, we used a Thermo Histowave II (made by Electron Beam Sciences) for many years for rush transplant biopsies. It worked Ok and the results were about 90% of the quality of regularly-processed tissue - however I think the slightly lessened quality was the shorter fixation time the bx had - maybe 2 hours from excision to starting processing. We did not do formalin fixation in the MW processor. The MW tissue processor part took about 15 minutes and required several manual changes of solutions, so was in a fume hood. It was more of a hassle to keep melted paraffin around than to run the processor. We kept the MW containers and paraffin ready in a dedicated oven. We used it daily so it was routine. The thing finally died and we now use a Peloris processor for Rush bx. The results are similar. Again, probably due to short fixation. MW processing should not be any scarier than any other processing. It simply requires validation, in which you define the results you expect and then work towards that in the workup. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dubansky, Benjamin Sent: Friday, September 27, 2013 1:51 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Microwave Processors Can some of you weigh in on how you feel about microwave histoprocessors? I am terrified that my samples will end up like raisins. "They" swear that these milestone processors are just as good as any other method, in terms of quality. I'ts all I have right now and it is very tempting to use it, although I am considering doing things the old fashioned way. And I am talking about pre-processor old fashioned way. I am that scared. Ben _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ratliffjack <@t> hotmail.com Mon Sep 30 07:49:29 2013 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Mon Sep 30 07:49:39 2013 Subject: [Histonet] Undecalcified sample in paraffin and plastic media In-Reply-To: References: Message-ID: <1C480BD1-5897-4998-8F58-F0636C015271@hotmail.com> Rui, You will definitely want to consider using plastic media like methyl methacrylate (MMA). It will cause less shrinkage in the tissue during polymerization, you can still cut at a range of 4-12 microns using a rotary microtome and tungsten-carbide knife, any mineralization present in the tissue will infiltrate and polymerize well allowing for enhanced stabilization of tissue and section morphology throughout microtomy, and you can even deplastify the sections with certain MMA formulations to increase staining options. Please let me know if you do wish to continue with plastic media as I have helped many labs to get started with and/or to refine their current capabilities with MMA. Additionally, I would like to point out that I Chair the Hard Tissue Committee (HTC) for the National Society for Histotechnology (NSH). Membership with the NSH has several benefits that could also help you to move forward with your project at your own pace. For example, as a member you will have access to all archived publications of the Journal of Histotechnology (JOH). With this access to the JOH via Manny Publishing, the HTC has created a reference document that collates all relevant publications (1970's to present) that pertain to bone, biomaterials, medical device implants, resin histology, etc., so that one can easily locate and obtain publication information relevant to their niche specific needs. Rest assured that I will be happy to help you either way you choose to move forward. Best Regards, Jack On Sep 23, 2013, at 9:19 PM, Rui TAHARA wrote: > > > I have undecalcified paraffin embed samples > that were sectioned at 10 micron that I want to stain with Von kossa. Because > samples are embryonic quail heads (ossification starts to happen) and still > soft enough to section with standard rotary microtome with tungsten knife in paraffin. > > > My intention is to 3D reconstruct anatomies > based on histological sections. Because of this, I am wondering if I should actually > use plastic media rather than paraffin to keep the section shape as consistent > as possible. Does plastic embed material actually preserve the consistent shape > among sections better than paraffin embed sample? No winkle etc..? Is there any > other advantage that I actually should use the plastic media than paraffin for what > I want to do? I know downside of plastic media is that in general plastic > embedding process are lengthy and plastic embedding material are expensive than > the paraffin ones, and are mainly use for bone to support the hard material for > sectioning. > > When I sectioned some ossified samples, beak > start to fall off from section and the section show the lines from the possibly > scratched knife. Is this indication of paraffin media that does not provide enough > strength for sectioning? I thought it may possibly the poor infiltration. > > > > In our lab nobody has processed the plastic > embedding and sectioning (we have only standard microtome, no vaccum machine. Can > I section plastic embed sample with the standard microtome at 10 micron?) so I would > like to have any input before actually making a plastic embed sample. Any > suggestions would be appreciated. > > > Rui TAHARA > Biology Department > McGill University > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TNMayer <@t> mdanderson.org Mon Sep 30 08:15:42 2013 From: TNMayer <@t> mdanderson.org (Mayer,Toysha N) Date: Mon Sep 30 08:15:51 2013 Subject: [Histonet] RE: Microwave Processors Message-ID: <47E9B2C01DDDD94881EACD2DC44EBC8802B5782A@D1PWPEXMBX05.mdanderson.edu> Tim is right, fixation is the key to microwave processing. Size of the tissue is very important for fixation, so be aware of that as well. Once you work out the times per change of reagent the tissues should be just fine. To avoid being concerned about the melted paraffin, invest in a paraffin tank. You know like the big coffee pot dispensers. It will be well worth it in the end. One other thing is to make sure when you dispose of the alcohol that you do not accidentally get water on the tissue. I used to pour the alcohol down the drain (before we had to put it in a waste container) and would wash it down with water, let's just say the tissue got water on them and the results were bad. MW are good for same day biopsies because they can shorten the TAT. It works great for GI bx. Sincerely, Toysha N. Mayer, MBA, HT(ASCP) tnmayer@mdanderson.org Instructor/Education Coordinator Program in Histotechnology School of Health Professions MD Anderson Cancer Center 713-563.3481 Message: 8 Date: Fri, 27 Sep 2013 22:12:06 +0000 From: "Morken, Timothy" Subject: [Histonet] RE: Microwave Processors To: "Dubansky, Benjamin" , "histonet@lists.utsouthwestern.edu" Message-ID: <761E2B5697F795489C8710BCC72141FF09B9E2@ex07.net.ucsf.edu> Content-Type: text/plain; charset=us-ascii Ben, we used a Thermo Histowave II (made by Electron Beam Sciences) for many years for rush transplant biopsies. It worked Ok and the results were about 90% of the quality of regularly-processed tissue - however I think the slightly lessened quality was the shorter fixation time the bx had - maybe 2 hours from excision to starting processing. We did not do formalin fixation in the MW processor. The MW tissue processor part took about 15 minutes and required several manual changes of solutions, so was in a fume hood. It was more of a hassle to keep melted paraffin around than to run the processor. We kept the MW containers and paraffin ready in a dedicated oven. We used it daily so it was routine. The thing finally died and we now use a Peloris processor for Rush bx. The results are similar. Again, probably due to short fixation. MW processing should not be any scarier than any other processing. It simply requires validation, in which you define the results you expect and then work towards that in the workup. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dubansky, Benjamin Sent: Friday, September 27, 2013 1:51 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Microwave Processors Can some of you weigh in on how you feel about microwave histoprocessors? I am terrified that my samples will end up like raisins. "They" swear that these milestone processors are just as good as any other method, in terms of quality. I'ts all I have right now and it is very tempting to use it, although I am considering doing things the old fashioned way. And I am talking about pre-processor old fashioned way. I am that scared. Ben _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 118, Issue 48 ***************************************** From ASelf <@t> georgetownhospitalsystem.org Mon Sep 30 09:32:59 2013 From: ASelf <@t> georgetownhospitalsystem.org (Amy Self) Date: Mon Sep 30 09:33:06 2013 Subject: [Histonet] HT Position in SC Message-ID: Georgetown Memorial Hospital is looking to hire an HT/HTL. This is a full time position with hours varying from 37.5 to 40.0 weekly. If anyone is interested and would like to apply please visit the Geogetown Memorial Hospital website for details or if you would like to speak with me for details I can be reached at 843-527-7179. Thanks, Amy Self Histology Lab Senior Tech Lab Georgetown Memorial Hospital 606 Black River Road Georgetown, SC 29440 843-520-8711 ASelf@georgetownhospitalsystem.org NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. From ree3 <@t> leicester.ac.uk Mon Sep 30 10:33:32 2013 From: ree3 <@t> leicester.ac.uk (Edwards, Richard E.) Date: Mon Sep 30 10:33:39 2013 Subject: [Histonet] Kimura stain, sorry a bit off topic Message-ID: <7722595275A4DD4FA225B92CDBF174A101B0D8D01B73@EXC-MBX3.cfs.le.ac.uk> Hi there, does anybody know the advantage of this stain over a straight toluidine blue stain for wbcs??, many thanks. Richard Edwards Leicester university U.K. From rizzaa <@t> uw.edu Mon Sep 30 11:49:28 2013 From: rizzaa <@t> uw.edu (Tony Rizzardi) Date: Mon Sep 30 11:51:46 2013 Subject: [Histonet] Beecher TMA arrayer service contract/calibration necessary? Message-ID: <002d01cebdfd$0725b3d0$15711b70$@uw.edu> We are currently using a Beecher MTA-1 tissue microarrayer and are wondering 1) if the equipment needs occasional calibration (and which vendors offer this service) 2) if any vendors offer service contracts for this equipment in case something breaks during routine use I've looked into several vendors already with no luck - any suggestions? Thanks! Tony Rizzardi University of Washington From srwilkes <@t> gmail.com Mon Sep 30 14:21:49 2013 From: srwilkes <@t> gmail.com (squigly144 .) Date: Mon Sep 30 14:21:55 2013 Subject: [Histonet] Stability of DAB signal Message-ID: Hi Histonetters! Can anyone point me in the direction of an article that tests the stability of DAB signal on a stained and coverslipped slide? There is a lot of literature about the stability of the DAB reagent, but I am having difficulty finding any reference to the stabiltiy of the signal/chromogen stability post staining/coverslipping, with slides stored under normal conditions. Has anyone performed these studies or has data to support stability studies? Thanks in advance! Steven srwilkes@gmail.com From MASCANLAN <@t> PARTNERS.ORG Mon Sep 30 14:51:56 2013 From: MASCANLAN <@t> PARTNERS.ORG (Scanlan, Megan Anne) Date: Mon Sep 30 14:52:02 2013 Subject: [Histonet] human lymph nodes from a cadaver Message-ID: <5D5B227A054CE346AE2EE3890005CDF81B0C5656@PHSX10MB9.partners.org> Hi all, Does anyone have experience cutting human lymph nodes from a cadaver in paraffin blocks? I am having quite a lot of trouble getting good sections, even using a brand new blade the sections end up with multiple blade marks streaking through the tissue and paraffin. I've contacted the researcher that gave me the samples to cut asking about how the cadaver was preserved and what fixatives they used. I'm thinking that the samples may not have been processed well enough since I've tried all the normal tricks for cutting paraffin's. There was one sample that seemed to have a calcium deposit or some very hardened tissue, not entirely sure but it nicked up the blade pretty good making sectioning impossible on that one. I'm not sure if the reason I am having trouble sectioning is because these came from a cadaver or because they are human lymph nodes, it is my first time cutting either of these things. Any and all advice and opinions are welcomed Thank you, Megan Megan Scanlan Research Technician I Wellman Center for Photomedicine Massachusetts General Hospital 50 Blossom Street, EDW 214 Boston, MA 02114 617-726-6983 (Lab) 781-635-6626 (Cell 726-1206 (fax) The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail.