[Histonet] RE: cutting thick brain frozen sections for Golgi stain

[Grantham, Andrea L - (algranth)]

I'm going to try sectioning at higher temps today and I hope that it works. Thanks all for the suggestions.
We used to have a vibratome but the thing only worked right for certain kinds of samples. I don't see us getting another one since the occasion to use something like that happens very infrequently. Too bad.
Yes, the brains were fixed - treated with mercury and have a gray-green cast to them (wearing gloves). They were also sucrose cryoprotected.


Andi




Andrea Grantham, HT (ASCP)
Senior Research Specialist
University of Arizona
Cellular and Molecular Medicine
Histology Service Laboratory
P.O.Box 245044
Tucson, AZ 85724

algranth <@t> email.arizona.edu<mailto:algranth <@t> email.arizona.edu>
Tel: 520.626.4415     Fax: 520.626.2097