From MASCANLAN <@t> PARTNERS.ORG Tue Oct 1 08:43:26 2013 From: MASCANLAN <@t> PARTNERS.ORG (Scanlan, Megan Anne) Date: Tue Oct 1 08:43:30 2013 Subject: [Histonet] human lymph nodes from a cadaver In-Reply-To: References: <5D5B227A054CE346AE2EE3890005CDF81B0C5656@PHSX10MB9.partners.org> Message-ID: <5D5B227A054CE346AE2EE3890005CDF81B0C56E4@PHSX10MB9.partners.org> Yup, that's standard with our cutting here but I will try giving them a lot more time on the ice bath once faced. Also at Linda Blazek we processed and embedded their tissue samples, so it's is already in the paraffin we use. Thank you, Megan Megan Scanlan Research Technician I Wellman Center for Photomedicine Massachusetts General Hospital 50 Blossom Street, EDW 214 Boston, MA 02114 617-726-6983 (Lab) 726-1206 (fax) -----Original Message----- From: Britton, Josette C [mailto:JCBRITTON@Cheshire-Med.COM] Sent: Tuesday, October 01, 2013 7:41 AM To: Scanlan, Megan Anne Subject: RE: [Histonet] human lymph nodes from a cadaver Face your blocks and soak them well in an ice water bath! Josie Britton HT (ASCP) QIHC Cheshire Medical Center Keene, NH 03431 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scanlan, Megan Anne Sent: Monday, September 30, 2013 3:52 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] human lymph nodes from a cadaver Hi all, Does anyone have experience cutting human lymph nodes from a cadaver in paraffin blocks? I am having quite a lot of trouble getting good sections, even using a brand new blade the sections end up with multiple blade marks streaking through the tissue and paraffin. I've contacted the researcher that gave me the samples to cut asking about how the cadaver was preserved and what fixatives they used. I'm thinking that the samples may not have been processed well enough since I've tried all the normal tricks for cutting paraffin's. There was one sample that seemed to have a calcium deposit or some very hardened tissue, not entirely sure but it nicked up the blade pretty good making sectioning impossible on that one. I'm not sure if the reason I am having trouble sectioning is because these came from a cadaver or because they are human lymph nodes, it is my first time cutting either of these things. Any and all advice and opinions are welcomed Thank you, Megan Megan Scanlan Research Technician I Wellman Center for Photomedicine Massachusetts General Hospital 50 Blossom Street, EDW 214 Boston, MA 02114 617-726-6983 (Lab) 781-635-6626 (Cell 726-1206 (fax) The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From rjr6 <@t> psu.edu Tue Oct 1 09:06:08 2013 From: rjr6 <@t> psu.edu (Roberta Horner) Date: Tue Oct 1 09:06:26 2013 Subject: [Histonet] Lab Chairs In-Reply-To: <3AD061FE740D464FAC7BF6B5CFB75707A8A6E24E@smcmail02.somerset-healthcare.com> References: <0BBA94171603A6468FFBA03A45A029FC0444C92FB7@MSEXCL.hscresex.local>, <5A33C952BB67F4468AF1F36D739212BCBD6EA58B@JERRY.Gia.com>, <3AD061FE740D464FAC7BF6B5CFB75707A8A6E24E@smcmail02.somerset-healthcare.com> Message-ID: I cannot find the company I originally order my saddle chairs from but the link below has a picture that looks a lot like what I have. The chairs I have are from CNS Chairs 800 Boylston St Suite 1600 Boston, MA 1-800-457-3120. Sorry it took a while to get the information I had to go back through my orders to find it. http://www.sitbetter.com/view/chair/ost-st205/backless-tall-stool-with-saddle-seat-and-seat-angle/?utm_campaign=shoppingengine&utm_medium=shoppingengine&utm_source=shopzilla&zmam=91792832&zmas=1&zmac=11&zmap=OST-ST205 Roberta Horner HT/HTL Penn State University Animal Diagnostic Lab -----Original Message----- From: Rathborne, Toni [mailto:trathborne@somerset-healthcare.com] Sent: Friday, September 27, 2013 7:36 AM To: Roberta Horner; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Lab Chairs Do you have a link to where we could see them? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Roberta Horner Sent: Friday, September 27, 2013 7:21 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Lab Chairs I have 2 saddle chairs and I really like them. You have to sit straight on them, no slouching. I have less back aches since I got them. Roberta Horner HT/HTL Penn State University Animal Diagnostic Lab _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek <@t> digestivespecialists.com Tue Oct 1 09:17:52 2013 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Tue Oct 1 09:18:02 2013 Subject: [Histonet] Lab Chairs In-Reply-To: References: <0BBA94171603A6468FFBA03A45A029FC0444C92FB7@MSEXCL.hscresex.local>, <5A33C952BB67F4468AF1F36D739212BCBD6EA58B@JERRY.Gia.com>, <3AD061FE740D464FAC7BF6B5CFB75707A8A6E24E@smcmail02.somerset-healthcare.com> Message-ID: <5A2BD13465E061429D6455C8D6B40E391661BCAF97@IBMB7Exchange.digestivespecialists.com> Mercedes Medical has something like that too. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Roberta Horner Sent: Tuesday, October 01, 2013 10:06 AM To: Rathborne, Toni; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Lab Chairs I cannot find the company I originally order my saddle chairs from but the link below has a picture that looks a lot like what I have. The chairs I have are from CNS Chairs 800 Boylston St Suite 1600 Boston, MA 1-800-457-3120. Sorry it took a while to get the information I had to go back through my orders to find it. http://www.sitbetter.com/view/chair/ost-st205/backless-tall-stool-with-saddle-seat-and-seat-angle/?utm_campaign=shoppingengine&utm_medium=shoppingengine&utm_source=shopzilla&zmam=91792832&zmas=1&zmac=11&zmap=OST-ST205 Roberta Horner HT/HTL Penn State University Animal Diagnostic Lab -----Original Message----- From: Rathborne, Toni [mailto:trathborne@somerset-healthcare.com] Sent: Friday, September 27, 2013 7:36 AM To: Roberta Horner; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Lab Chairs Do you have a link to where we could see them? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Roberta Horner Sent: Friday, September 27, 2013 7:21 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Lab Chairs I have 2 saddle chairs and I really like them. You have to sit straight on them, no slouching. I have less back aches since I got them. Roberta Horner HT/HTL Penn State University Animal Diagnostic Lab _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From flnails <@t> texaschildrens.org Tue Oct 1 09:27:06 2013 From: flnails <@t> texaschildrens.org (Nails, Felton) Date: Tue Oct 1 09:27:33 2013 Subject: [Histonet] BK Virus Message-ID: <327E034F1892504289B7A17EC71DF9F303D308@TCFMSG03.ad.texaschildrenshospital.org> Any ideas in histoland where I can find control tissue for BK Virus IHC? ______________________________________________________________________ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ______________________________________________________________________ From TMcNemar <@t> lmhealth.org Tue Oct 1 10:00:00 2013 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Tue Oct 1 10:00:18 2013 Subject: [Histonet] IHC trun-around-time Message-ID: Hello all, I am wondering how many places offer same-day staining of IHC. We are a small hospital based lab that averages about 70 IHC slides per week. We operate 5 days/week, 0600-1600. We give same day staining for IHC requests that are received by 10am and are using the Ventana Benchmark XT. Due to some issues that have developed, we may have to change the way we do things. With our current 10am cutoff plus possible pre-analytical changes the instrument will finish too late in the afternoon. The pathologists really like the same day service on IHC but I'm thinking that I may have to make the cutoff time earlier and anything after that will just have to wait. So I am wondering if anyone else of similar size, instrumentation, and workload, offers same-day staining for IHC. I appreciate your input. Thanks. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org ________________________________ This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From rjbuesa <@t> yahoo.com Tue Oct 1 10:10:18 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Oct 1 10:10:24 2013 Subject: [Histonet] human lymph nodes from a cadaver In-Reply-To: <5D5B227A054CE346AE2EE3890005CDF81B0C5656@PHSX10MB9.partners.org> References: <5D5B227A054CE346AE2EE3890005CDF81B0C5656@PHSX10MB9.partners.org> Message-ID: <1380640218.54520.YahooMailNeo@web163102.mail.bf1.yahoo.com> Your problems may reside on the time it took between death and autopsy. If more than 12 hours the LN may have started decay and fixation on these circumstances will be very problematic compromising the whole process after wards. That could be the root of your problems because if autopsy takes place soon the LN will present no problems Ren? J. ________________________________ From: "Scanlan, Megan Anne" To: "'histonet@lists.utsouthwestern.edu'" Sent: Monday, September 30, 2013 3:51 PM Subject: [Histonet] human lymph nodes from a cadaver Hi all, Does anyone have experience cutting human lymph nodes from a cadaver in paraffin blocks?? I am having quite a lot of trouble getting good sections, even using a brand new blade the sections end up with multiple blade marks streaking through the tissue and paraffin. I've contacted the researcher that gave me the samples to cut asking about how the cadaver was preserved and what fixatives they used.? I'm thinking that the samples may not have been processed well enough since I've tried all the normal tricks for cutting paraffin's.? There was one sample that seemed to have a calcium deposit or some very hardened tissue, not entirely sure but it nicked up the blade pretty good making sectioning impossible on that one. I'm not sure if the reason I am having trouble sectioning is because these came from a cadaver or because they are human lymph nodes, it is my first time cutting either of these things. Any and all advice and opinions are welcomed Thank you, ? ? Megan Megan Scanlan Research Technician I Wellman Center for Photomedicine Massachusetts General Hospital 50 Blossom Street, EDW 214 Boston, MA 02114 617-726-6983 (Lab) 781-635-6626 (Cell 726-1206 (fax) The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline. If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Oct 1 10:19:03 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Oct 1 10:19:07 2013 Subject: [Histonet] IHC trun-around-time In-Reply-To: References: Message-ID: <1380640743.58422.YahooMailNeo@web163104.mail.bf1.yahoo.com> >From what you wrote I imagine that the 10AM cutoff is for the pathologists requests from cases read the previous day, right? If that is the case, the pathologists are pushing to do something when the root of the problem is their diagnosis requiring IHC as confirmation tests. If the IHC are from same day cases that you gave them early in the morning and they read before 10AM I believe that your cutoff time is OK because you will need about 4h to complete the while cycle(recut? HIER?IHC procedure). I imagine that you are at the most 3 HTs and if that is the case you could also make the one doing the IHC to start at 0800?and finish at 1800 but in that case maybe the slides will not be on time. You have to work with the pathologists on this issue Ren? J.? ________________________________ From: Tom McNemar To: "histonet@lists.utsouthwestern.edu" Sent: Tuesday, October 1, 2013 11:00 AM Subject: [Histonet] IHC trun-around-time Hello all, I am wondering how many places offer same-day staining of IHC.? We are a small hospital based lab that averages about 70 IHC slides per week.? We operate 5 days/week, 0600-1600.? We give same day staining for IHC requests that are received by 10am and are using the Ventana Benchmark XT.? Due to some issues that have developed, we may have to change the way we do things. With our current 10am cutoff plus possible pre-analytical changes the instrument will finish too late in the afternoon.? The pathologists really like the same day service on IHC but I'm thinking that I may have to make the cutoff time earlier and anything after that will just have to wait. So I am wondering if anyone else of similar size, instrumentation, and workload, offers same-day staining for IHC.? I appreciate your input.? Thanks. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org ________________________________ This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Tue Oct 1 11:07:19 2013 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Tue Oct 1 11:07:33 2013 Subject: [Histonet] thawing and refreezing muscle? Message-ID: <761E2B5697F795489C8710BCC72141FF09BE2F@ex07.net.ucsf.edu> Histonetters, Does anyone have a good method for thawing muscle and refreezing for histochemistry? With good results? We have some bulk-frozen muscle (centimeter thick) and that is what they want to try. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center From Fawn.Bomar <@t> HalifaxRegional.com Tue Oct 1 12:06:53 2013 From: Fawn.Bomar <@t> HalifaxRegional.com (Fawn Bomar) Date: Tue Oct 1 12:07:05 2013 Subject: [Histonet] Validating new Benchmark instrument Message-ID: <0111BC10D77DC54EAB99B2DDA3BCE4B95A59BB@EXCH-2K10.hrhs.com> Hi everyone, I know that this subject has come up several times and I am sorry to bother everyone again but I was hoping to find some help. We are upgrading our Benchmark Immuno machines to the Benchmark XT immuno machine at the end of October. Does anyone have any suggestions on how to approach the validation process? Do we need to validate every antibody that we use as well as the kits? I know that there is also a gray area surrounding how many slides need to be run to consider the antibody validated, but what would be the suggested amount of slides to validate a new machine as I know this will be very costly. Does anybody have a log/checklist/spreadsheet that they would be willing to share to help document this validation of the machine and each of the antibodies? Thank you in advance for your help Fawn Bomar ------------------------------------------------------------- This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you From oneilb <@t> wvuhealthcare.com Tue Oct 1 12:11:27 2013 From: oneilb <@t> wvuhealthcare.com (Oneil, Beth Ann) Date: Tue Oct 1 12:11:35 2013 Subject: [Histonet] RE: IHC TAT Message-ID: <3CEB8EBCF9C7A648B9694B5696462A71287F6593@NT-EXMB2.wvuh.wvuhs.com> We previously had two Ventana Benchmarks and was only able to get one set of stains off per day. We had an 11 am cut-off which worked well. We would do overnight stains as well so at least we were able to take off stains the following morning. We replaced the Benchmarks with two Leica Bond III's and can get almost continuous flow with IHC. Beth Ann O'Neil, MT(ASCP)SC, HTL, QIHC Histology Supervisor/Technical Specialist West Virginia University Hospitals oneilb@wvuhealthcare.com 304-293-7629 (office) 304-293-6014 (lab) From LSebree <@t> uwhealth.org Tue Oct 1 12:11:42 2013 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Tue Oct 1 12:11:46 2013 Subject: [Histonet] RE: IHC trun-around-time In-Reply-To: References: Message-ID: <77DD817201982748BC67D7960F2F76AF069F22@UWHC-MBX12.uwhis.hosp.wisc.edu> Hey Tom, Although we have a higher workload than you, ~ 1500 slides/month, we offer same day TAT with a cut-off of noon. There are a couple exceptions, i.e. triple stains and HER2 dual ISH but otherwise, utilizing 4 Ventana Ultras, we have it all out by 4 pm or slightly later. Our hours are 5 am to 4 pm with 2.25 FTEs. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Tuesday, October 01, 2013 10:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC trun-around-time Hello all, I am wondering how many places offer same-day staining of IHC. We are a small hospital based lab that averages about 70 IHC slides per week. We operate 5 days/week, 0600-1600. We give same day staining for IHC requests that are received by 10am and are using the Ventana Benchmark XT. Due to some issues that have developed, we may have to change the way we do things. With our current 10am cutoff plus possible pre-analytical changes the instrument will finish too late in the afternoon. The pathologists really like the same day service on IHC but I'm thinking that I may have to make the cutoff time earlier and anything after that will just have to wait. So I am wondering if anyone else of similar size, instrumentation, and workload, offers same-day staining for IHC. I appreciate your input. Thanks. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org ________________________________ This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From McKenzie.Emily <@t> mhsil.com Tue Oct 1 12:15:01 2013 From: McKenzie.Emily <@t> mhsil.com (McKenzie, Emily) Date: Tue Oct 1 12:15:07 2013 Subject: [Histonet] IHC turnaround time Message-ID: Hello all, I am currently working on a Six Sigma Green Belt project and am looking for some data. Can any one give me their general turnaround times for IHC, from time of order to time of delivery? Thanks for all your help with this, Emily K. McKenzie BS, HT(ASCP) Memorial Medical Center?701 North First Street?Springfield, IL 62781 Ph: 217-788-3991?email: McKenzie.Emily@mhsil.com ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From gu.lang <@t> gmx.at Tue Oct 1 12:27:20 2013 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Tue Oct 1 12:29:02 2013 Subject: AW: [Histonet] IHC trun-around-time In-Reply-To: References: Message-ID: <000901cebecb$7c19d7b0$744d8710$@gmx.at> Hi Tom, we also do same-day-IHC. Our cut off is 10.30. We use Benchmark ultra with ultravision-kit. The run is started between 10.30-10.50 and needs about 3 hours (more or less). After the run is completed we need about 30 hours until delivering the slides. For faster processing we have an "online" order service for IHC. The doctors fill a Word-form and print it on our lab-printer. So the orders go in continously. The urgent cases (mamma-biopsies eg.) are cut in advance with the HE and relatively fast prepared for IHC. Usually the slides need no drying time in the oven. What issues? What changes? Regards Gudrun Lang -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Tom McNemar Gesendet: Dienstag, 01. Oktober 2013 17:00 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] IHC trun-around-time Hello all, I am wondering how many places offer same-day staining of IHC. We are a small hospital based lab that averages about 70 IHC slides per week. We operate 5 days/week, 0600-1600. We give same day staining for IHC requests that are received by 10am and are using the Ventana Benchmark XT. Due to some issues that have developed, we may have to change the way we do things. With our current 10am cutoff plus possible pre-analytical changes the instrument will finish too late in the afternoon. The pathologists really like the same day service on IHC but I'm thinking that I may have to make the cutoff time earlier and anything after that will just have to wait. So I am wondering if anyone else of similar size, instrumentation, and workload, offers same-day staining for IHC. I appreciate your input. Thanks. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org ________________________________ This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wbenton <@t> cua.md Tue Oct 1 12:46:47 2013 From: wbenton <@t> cua.md (Walter Benton) Date: Tue Oct 1 12:49:51 2013 Subject: [Histonet] RE: thawing and refreezing muscle? In-Reply-To: <761E2B5697F795489C8710BCC72141FF09BE2F@ex07.net.ucsf.edu> References: <761E2B5697F795489C8710BCC72141FF09BE2F@ex07.net.ucsf.edu> Message-ID: <0B8979A204680A42B93A52B486088CD939004207FF@CUAEXH1.GCU-MD.local> This may help. Never used it when I processed fresh/frozen muscle specimens but have heard good things. http://www.cell-ess.com/Nonfrozen_Transport_Medium_Preserves_and_Restores_Skeletal_Muscle_Enzymatic_Activity_and_Morphology.pdf http://www.lifebloodmedical.com/ Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) ChesapeakeUrology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy [Timothy.Morken@ucsfmedctr.org] Sent: Tuesday, October 01, 2013 12:07 PM To: Histonet Cc: jmitchell@neurology.wisc.edu Subject: [Histonet] thawing and refreezing muscle? Histonetters, Does anyone have a good method for thawing muscle and refreezing for histochemistry? With good results? We have some bulk-frozen muscle (centimeter thick) and that is what they want to try. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From joelleweaver <@t> hotmail.com Tue Oct 1 13:01:40 2013 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Tue Oct 1 13:01:46 2013 Subject: [Histonet] Validating new Benchmark instrument In-Reply-To: <0111BC10D77DC54EAB99B2DDA3BCE4B95A59BB@EXCH-2K10.hrhs.com> References: <0111BC10D77DC54EAB99B2DDA3BCE4B95A59BB@EXCH-2K10.hrhs.com> Message-ID: Fawn Unfortunately you have to validate each with new instrumentation, platform, change, fixation/processing alteration etc. I do at least 10 cases for IVD and 20 or so for ASR. You may be able to do less than your normal validation process and amount of slides for a completely new AB, since you are just trying to show that your previously validated AB works the same as your new platform/instrument. See what your medical director requires. You will have the information from your previous validation studies, titers, cases, etc to help make it a little more streamlined. It should be easier since you are using the same vendor, and probably the same clones and all that. You have some reports in the Ventana instruments that you might be able use to migrate over data. This might be more useful than using someone else's spreadsheet or data, especially if they don't use Ventana. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: Fawn.Bomar@HalifaxRegional.com > To: histonet@lists.utsouthwestern.edu > Date: Tue, 1 Oct 2013 17:06:53 +0000 > Subject: [Histonet] Validating new Benchmark instrument > > Hi everyone, > > > > I know that this subject has come up several times and I am sorry to bother everyone again but I was hoping to find some help. We are upgrading our Benchmark Immuno machines to the Benchmark XT immuno machine at the end of October. Does anyone have any suggestions on how to approach the validation process? Do we need to validate every antibody that we use as well as the kits? I know that there is also a gray area surrounding how many slides need to be run to consider the antibody validated, but what would be the suggested amount of slides to validate a new machine as I know this will be very costly. Does anybody have a log/checklist/spreadsheet that they would be willing to share to help document this validation of the machine and each of the antibodies? > > > > Thank you in advance for your help > > > > Fawn Bomar > ------------------------------------------------------------- > This electronic message may contain information that is > confidential or legally privileged. It is intended only > for the use of the individual(s) and entity named as recipients > in the message. > > If you are not an intended recipient of this message, please > notify the sender immediately and delete the material from any > computer. Do not deliver, distribute, or copy this message, and > do not disclose its contents or take any action in reliance on > the information it contains. > > Thank you > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Bauer.Karen <@t> mayo.edu Tue Oct 1 14:22:35 2013 From: Bauer.Karen <@t> mayo.edu (Bauer, Karen L.) Date: Tue Oct 1 14:22:39 2013 Subject: [Histonet] MOHS IPs Message-ID: <8A2A8E67E35BB24DAD9375F9E16310750E1160@MSGPEXCHA01A.mfad.mfroot.org> Hi all, We are in the process of validating some antibodies in our MOHS lab. The Melan A (Mart 1) antibody is working well, but it could be darker. We will be increasing the Ab incubation time to see if that will help. As for the Pan Keratin, we cannot get it to work at all. We use Protease 2 on our Ultra stainer for FFPE tissues in Histology, but this stain in MOHS is placed on fresh, unfixed tissue... by a manual drop method. Any time we've tried to use an enzyme retrieval, the tissue looks eaten up... even if we incubate if for a minute. Since the tissue is not fixed, I figured that no retrieval step would be needed, but the Pan Keratin refuses to work with or without retrieval. For those of you in MOHS labs that are using a manual staining method for Melan A and Pan Keratin, would you be willing to share your protocol with us? Thanks so much!! Karen Karen L. Bauer HTL/HT (ASCP) | Histology Supervisor | Pathology | MOHS Lab Supervisor | Dermatology | Phone: 715-838-3205 | bauer.karen@mayo.edu | Mayo Clinic Health System | 1221 Whipple Street | Eau Claire, WI 54702 | mayoclinichealthsystem.org From Elizabeth.Cameron <@t> jax.org Tue Oct 1 14:31:47 2013 From: Elizabeth.Cameron <@t> jax.org (Elizabeth Cameron) Date: Tue Oct 1 14:31:52 2013 Subject: [Histonet] Finetec Cryofilm? Message-ID: Hi all, Does anyone know of a supplier that carries Finetec Cryofilm? I can't seem to find anything. If anyone has used this, can you tell me how it compares to CryoJane tape? Thank you, Liz The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as possible. From hhawkins <@t> UTMB.EDU Tue Oct 1 15:35:23 2013 From: hhawkins <@t> UTMB.EDU (Hawkins, Hal K.) Date: Tue Oct 1 15:35:43 2013 Subject: [Histonet] RE: thawing and refreezing muscle? In-Reply-To: <0B8979A204680A42B93A52B486088CD939004207FF@CUAEXH1.GCU-MD.local> References: <761E2B5697F795489C8710BCC72141FF09BE2F@ex07.net.ucsf.edu>, <0B8979A204680A42B93A52B486088CD939004207FF@CUAEXH1.GCU-MD.local> Message-ID: <22624908330375439D6382C9F95093FF28DE01DB@GRMBX1.utmb.edu> We have had reasonable results based on rapid thawing of frozen samples in room temperature buffer followed by formalin fixation or rapid freezing in OCT. With such large samples, though, there are bound to be large ice crystals in the interior of the specimen regardless of the method used for freezing. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Walter Benton [wbenton@cua.md] Sent: Tuesday, October 01, 2013 12:46 PM To: Morken, Timothy; Histonet Cc: jmitchell@neurology.wisc.edu Subject: [Histonet] RE: thawing and refreezing muscle? This may help. Never used it when I processed fresh/frozen muscle specimens but have heard good things. http://www.cell-ess.com/Nonfrozen_Transport_Medium_Preserves_and_Restores_Skeletal_Muscle_Enzymatic_Activity_and_Morphology.pdf http://www.lifebloodmedical.com/ Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) ChesapeakeUrology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy [Timothy.Morken@ucsfmedctr.org] Sent: Tuesday, October 01, 2013 12:07 PM To: Histonet Cc: jmitchell@neurology.wisc.edu Subject: [Histonet] thawing and refreezing muscle? Histonetters, Does anyone have a good method for thawing muscle and refreezing for histochemistry? With good results? We have some bulk-frozen muscle (centimeter thick) and that is what they want to try. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From avogaro <@t> science.unitn.it Tue Oct 1 16:42:36 2013 From: avogaro <@t> science.unitn.it (Laura Avogaro) Date: Tue Oct 1 16:42:45 2013 Subject: [Histonet] RE: thawing and refreezing muscle? In-Reply-To: <22624908330375439D6382C9F95093FF28DE01DB@GRMBX1.utmb.edu> References: <761E2B5697F795489C8710BCC72141FF09BE2F@ex07.net.ucsf.edu>, <0B8979A204680A42B93A52B486088CD939004207FF@CUAEXH1.GCU-MD.local> <22624908330375439D6382C9F95093FF28DE01DB@GRMBX1.utmb.edu> Message-ID: <57123.87.14.0.247.1380663756.squirrel@www.science.unitn.it> I would like to fix my muscle tissue samples in formalin after freezing in OCT. Does anyone have experience or could give me some suggestion? I would like to avoid artifacts... Laura Avogaro Centre for Integrative Biology University of Trento Via delle Regole, 101 38123 Mattarello (TN) ? Italy > > We have had reasonable results based on rapid thawing of frozen samples in > room temperature buffer followed by formalin fixation or rapid freezing in > OCT. With such large samples, though, there are bound to be large ice crystals in the interior of the specimen regardless of the method used for > freezing. > > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu > [histonet-bounces@lists.utsouthwestern.edu] on behalf of Walter Benton [wbenton@cua.md] > Sent: Tuesday, October 01, 2013 12:46 PM > To: Morken, Timothy; Histonet > Cc: jmitchell@neurology.wisc.edu > Subject: [Histonet] RE: thawing and refreezing muscle? > > This may help. Never used it when I processed fresh/frozen muscle specimens but have heard good things. > > http://www.cell-ess.com/Nonfrozen_Transport_Medium_Preserves_and_Restores_Skeletal_Muscle_Enzymatic_Activity_and_Morphology.pdf > > http://www.lifebloodmedical.com/ > > Walter Benton HT(ASCP)QIHC > Histology Supervisor > Chesapeake Urology Associates > 806 Landmark Drive, Suite 127 > Glen Burnie, MD 21061 > 443-471-5850 (Direct) > 410-768-5961 (Lab) > 410-768-5965 (Fax) > ChesapeakeUrology.com > > Voted a Best Place to Work by > Baltimore and Modern Healthcare > Magazines. > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu > [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy [Timothy.Morken@ucsfmedctr.org] > Sent: Tuesday, October 01, 2013 12:07 PM > To: Histonet > Cc: jmitchell@neurology.wisc.edu > Subject: [Histonet] thawing and refreezing muscle? > > Histonetters, Does anyone have a good method for thawing muscle and refreezing for histochemistry? With good results? We have some bulk-frozen > muscle (centimeter thick) and that is what they want to try. > > Tim Morken > Supervisor, Electron Microscopy/Neuromuscular Special Studies > Department of Pathology > UC San Francisco Medical Center > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it > to the intended recipient, you are hereby notified that any dissemination, > distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the > transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Timothy.Morken <@t> ucsfmedctr.org Tue Oct 1 17:23:04 2013 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Tue Oct 1 17:23:22 2013 Subject: [Histonet] Thanks! RE: thawing and refreezing muscle? Message-ID: <761E2B5697F795489C8710BCC72141FF09BFCB@ex07.net.ucsf.edu> Thanks to everyone for their muscle refreezing methods. We ended up being able to quickly split the samples and cut without thawing. But we will try some out for the future, just in case. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Hawkins, Hal K. [mailto:hhawkins@UTMB.EDU] Sent: Tuesday, October 01, 2013 1:35 PM To: Walter Benton; Morken, Timothy; Histonet Cc: jmitchell@neurology.wisc.edu Subject: RE: thawing and refreezing muscle? We have had reasonable results based on rapid thawing of frozen samples in room temperature buffer followed by formalin fixation or rapid freezing in OCT. With such large samples, though, there are bound to be large ice crystals in the interior of the specimen regardless of the method used for freezing. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Walter Benton [wbenton@cua.md] Sent: Tuesday, October 01, 2013 12:46 PM To: Morken, Timothy; Histonet Cc: jmitchell@neurology.wisc.edu Subject: [Histonet] RE: thawing and refreezing muscle? This may help. Never used it when I processed fresh/frozen muscle specimens but have heard good things. http://www.cell-ess.com/Nonfrozen_Transport_Medium_Preserves_and_Restores_Skeletal_Muscle_Enzymatic_Activity_and_Morphology.pdf http://www.lifebloodmedical.com/ Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) ChesapeakeUrology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy [Timothy.Morken@ucsfmedctr.org] Sent: Tuesday, October 01, 2013 12:07 PM To: Histonet Cc: jmitchell@neurology.wisc.edu Subject: [Histonet] thawing and refreezing muscle? Histonetters, Does anyone have a good method for thawing muscle and refreezing for histochemistry? With good results? We have some bulk-frozen muscle (centimeter thick) and that is what they want to try. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Tony_Reilly <@t> health.qld.gov.au Tue Oct 1 18:15:18 2013 From: Tony_Reilly <@t> health.qld.gov.au (Tony Reilly) Date: Tue Oct 1 18:15:43 2013 Subject: [Histonet] thawing and refreezing muscle? In-Reply-To: <761E2B5697F795489C8710BCC72141FF09BE2F@ex07.net.ucsf.edu> References: <761E2B5697F795489C8710BCC72141FF09BE2F@ex07.net.ucsf.edu> Message-ID: <524BE427.411C.0039.0@health.qld.gov.au> Hi Tim A guy in our histology group gave a presentation debunking the theory that ice crystal artefact is irreversible. His lab receives frozen specimens from thousands of kilometres away from labs with no histology staff. If there was ice crystal artefact he defrosts the specimen at room temperature. Mop the specimen with tissues to remove as much OCT and moisture as possible. Allow the specimen to remain at room temperature on the bench for 20-30 minutes depending on the size of the specimen to allow evaporation of more moisture. Refreeze the specimen with OCT and cut sections. The result was absence of ice crystal artefact but some separation of muscle bundles which does not interfere with staining. It appears the ice crystals do not damage the fibres just cause separation. So removing the moisture eliminates the problem. If you have a lot of muscle perhaps you could separate a small amount to trial the procedure. regards Tony Tony Reilly B.App.Sc. , M.Sc. Chief Scientist, Anatomical Pathology Pathology Queensland-PA Laboratory ________________________________________________ Health Services Support Agency | Department of Health Level 1, Building 15,Princess Alexandra Hospital Ipswich Road,WOOLLOONGABBA Qld4102 Ph: 07 3176 2412 Mob: 0402 139411 Fax: 07 3176 2930 Email: tony_reilly@health.qld.gov.au Web: www.health.qld.gov.au/qhcss/ >>> "Morken, Timothy" 10/2/2013 2:07 am >>> Histonetters, Does anyone have a good method for thawing muscle and refreezing for histochemistry? With good results? We have some bulk-frozen muscle (centimeter thick) and that is what they want to try. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. 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Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ********************************************************************************** From ggracie <@t> stvincents.com.au Tue Oct 1 18:53:04 2013 From: ggracie <@t> stvincents.com.au (Gary Gracie) Date: Tue Oct 1 18:53:14 2013 Subject: [Histonet] IHC turn around time Message-ID: <00d2901d71df42319978e29cc95d55fb@SVMHSEXCH02.svmhs.stvincents.com.au> Dear Tom Upgrade to the benchmark Ultra and your problem is solved as you can add and remove slides continuously throughout the day. There is no "run" and no need for cut offs. Our upgrade was cost neutral. Regards Gary Gracie Senior Technical Officer IHC Laboratory Anatomical Pathology St Vincents Hospital Sydney, Australia Hello all, I am wondering how many places offer same-day staining of IHC. We are a small hospital based lab that averages about 70 IHC slides per week. We operate 5 days/week, 0600-1600. We give same day staining for IHC requests that are received by 10am and are using the Ventana Benchmark XT. Due to some issues that have developed, we may have to change the way we do things. With our current 10am cutoff plus possible pre-analytical changes the instrument will finish too late in the afternoon. The pathologists really like the same day service on IHC but I'm thinking that I may have to make the cutoff time earlier and anything after that will just have to wait. So I am wondering if anyone else of similar size, instrumentation, and workload, offers same-day staining for IHC. I appreciate your input. Thanks. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the system manager. This footnote also confirms that this email message has been virus scanned and although no viruses were detected by the system, St Vincents & Mater Health Sydney accepts no liability for any consequential damage resulting from email containing any computer viruses. ********************************************************************** From barbara.tibbs <@t> accuratediagnosticlabs.com Wed Oct 2 07:54:54 2013 From: barbara.tibbs <@t> accuratediagnosticlabs.com (Barbara Tibbs) Date: Wed Oct 2 07:55:21 2013 Subject: [Histonet] RE: MOHS IPs In-Reply-To: <8A2A8E67E35BB24DAD9375F9E16310750E1160@MSGPEXCHA01A.mfad.mfroot.org> References: <8A2A8E67E35BB24DAD9375F9E16310750E1160@MSGPEXCHA01A.mfad.mfroot.org> Message-ID: <18e2dbbd42434929b3cffaff78630b24@BL2PR04MB196.namprd04.prod.outlook.com> Hello Karen, Back in the old days of doing IHC manually on mostly fresh, frozen tissue I would immediately place the slide with the frozen section into a coplin jar of acetone to fix it. Keep the coplin jar of acetone in the cryostat. Leave it in the acetone for only 10 minutes. Try diluting the Protease 2 - 1:1 to make it gentler so it doesn't "eat" the tissue. This technique worked well when I was doing ER/PR on frozen breast tumors. Not sure it will work with skin but it's worth a try. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.tibbs@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Bauer, Karen L. Sent: Tuesday, October 01, 2013 5:22 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] MOHS IPs Hi all, We are in the process of validating some antibodies in our MOHS lab. The Melan A (Mart 1) antibody is working well, but it could be darker. We will be increasing the Ab incubation time to see if that will help. As for the Pan Keratin, we cannot get it to work at all. We use Protease 2 on our Ultra stainer for FFPE tissues in Histology, but this stain in MOHS is placed on fresh, unfixed tissue... by a manual drop method. Any time we've tried to use an enzyme retrieval, the tissue looks eaten up... even if we incubate if for a minute. Since the tissue is not fixed, I figured that no retrieval step would be needed, but the Pan Keratin refuses to work with or without retrieval. For those of you in MOHS labs that are using a manual staining method for Melan A and Pan Keratin, would you be willing to share your protocol with us? Thanks so much!! Karen Karen L. Bauer HTL/HT (ASCP) | Histology Supervisor | Pathology | MOHS Lab Supervisor | Dermatology | Phone: 715-838-3205 | bauer.karen@mayo.edu | Mayo Clinic Health System | 1221 Whipple Street | Eau Claire, WI 54702 | mayoclinichealthsystem.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Wed Oct 2 09:35:33 2013 From: pruegg <@t> ihctech.net (pruegg@ihctech.net) Date: Wed Oct 2 09:35:40 2013 Subject: [Histonet] RE: MOHS IPs Message-ID: <20131002073533.f86bd30e73b823f57b516b5451216a98.951d862201.wbe@email01.secureserver.net> I agree with Barbara try fixing in cold acetone/ethanol mix even just for a couple of minutes then go directly to buffer do not dry after what ever u u frozen sections i enzyme should help prevent ov incubating the primary? Are u usin detection? HRP/Dab or AEC or Alk.P/fast -------- Original Message -------- Subject: [Histonet] RE: MOHS From: Barbara Tibbs <[1]barbara.tibbs@accuratediagnosticlabs.com> D To: "Bauer, Karen L." <[2]Bauer.Karen@mayo.edu>, "'histonet@lists.utsouthwest <[3]histonet@lists.utsouthwestern.edu> Hello Karen, Back in the old days of doing IHC manually on mostly fresh, froze n tissue I would immediately place the slide with the frozen section into a acetone in the cr minutes. Try diluting the Pr doesn't "eat" the tissue. This te doing ER/PR on frozen breast tumors. Not su but it's worth a try. Barbara S. Tib Histology Supervisor Accurate Diagnostic Labs South Pl [4]barbara.tibbs@accuratediagnosticlabs.com 732-839-3374 C _____________________________________ From: [5]histonet-bounces@lists.utsouthwestern.edu <[6]histonet-bounces@lists.utsouthwestern Karen L. <[7]Bauer.Karen@mayo.edu> Sent: Tuesday, October 01, 20 To: '[8] Subject: [Histonet] MOHS IPs Hi all, We are in the process of validating some a lab. The Melan A (Mart 1) antibody is working well, darker. We will be increasing the Ab incubation time to se will help. As for the Pan Keratin, we cannot get it Protease 2 on our Ultra stainer for FFPE tissues in this stain in MOHS is placed on fresh, unfixed tissue... by drop method. Any time we've tried to use an enzyme retrieval, th tissue looks eaten up... even if we incubate if for a minute. would be retrieval. For those of you in MOHS labs that are using a manual staining me thod for Melan A and Pan Keratin, would you be willing to share your protoc Thanks so much!! Karen K MOHS Lab S [9]bauer.karen@mayo.edu<[10]mailto:bauer.karen@mayo.edu> | Mayo Clinic Health System | 122 [11]mayoclinichealthsystem.org _______________________________________________ Histonet ma [13]Histo [14]http://lists.utsouthwestern.edu/mailman/l _________________________________________ Histonet mailing list [15]Histonet@lists.utsouthwestern.edu [16]http://lists.utso References 1. 3D"mailto:barbara.tibbs@accurated 2. ="mailto:Bauer.Karen@mayo.edu" 3. 3D"mailto:histonet@lists.utsouthwestern.edu 4. 3D"mailto:barbara.tibbs@accuratediagnosticlabs.c 5. 3D"mailto:histonet-bounces@lists.utsouthwestern.edu 6. 3D"mailto:histo 7. 3D"mailto:Bauer.Karen 8. 3D"mailto:histonet@lists.utsouthwestern.edu" 9. 3D"mailto:bauer.kar 10. 3D"mailto:bauer.karen@mayo 11. 3D"http://mayoclinichealt=/ 12. 3D"http:/ 13. 3D"mailto:Histonet@lists.utsouthwestern.edu" 14. 3D"http://lists.utsouthweste=/ 15. 3D"mailto:Histonet@lists.u 16. file://localhost/tmp/3D"h From Bauer.Karen <@t> mayo.edu Wed Oct 2 09:47:26 2013 From: Bauer.Karen <@t> mayo.edu (Bauer, Karen L.) Date: Wed Oct 2 09:47:43 2013 Subject: [Histonet] RE: MOHS IPs In-Reply-To: <20131002073533.f86bd30e73b823f57b516b5451216a98.951d862201.wbe@email01.secureserver.net> References: <20131002073533.f86bd30e73b823f57b516b5451216a98.951d862201.wbe@email01.secureserver.net> Message-ID: <8A2A8E67E35BB24DAD9375F9E16310750E3549@MSGPEXCHA01A.mfad.mfroot.org> Thanks for the reply, We are pre-fixing in a formalin substitute, but we'll give the acetone a try. I have diluted the protease, but have not gotten any good results. We'll keep trying with that as well. We've tried multiple incubation times for the cytokeratin... From 5 minutes all the way up to 30 minutes... With no luck. We are using an enhanced polymer DAB. Thanks for the suggestions! I greatly appreciate it! :) Karen Karen L. Bauer HTL/HT (ASCP) | Histology Supervisor | Pathology | MOHS Lab Supervisor | Dermatology | Phone: 715-838-3205 | bauer.karen@mayo.edu | Mayo Clinic Health System | 1221 Whipple Street | Eau Claire, WI 54702 | mayoclinichealthsystem.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of pruegg@ihctech.net Sent: Wednesday, October 02, 2013 9:36 AM To: Barbara Tibbs; Bauer, Karen L.; 'histonet@lists.utsouthwestern.edu' Subject: RE: [Histonet] RE: MOHS IPs I agree with Barbara try fixing in cold acetone/ethanol mix even = just for a couple of minutes then go directly to buffer do not dry after=x, I would dilute the protease 1:4 with buffer (PBS or TBS what ever u u=) and do it for a short time. Enzyme digestion on frozen sections i=ricky but fixing a little and diluting the enzyme should help prevent ov= digestion. How long are u incubating the primary? Are u usin=n enhanced labeled polymer detection? HRP/Dab or AEC or Alk.P/fast=d? -------- Original Message -------- Subject: [Histonet] RE: MOHS =s From: Barbara Tibbs <[1]barbara.tibbs@accuratediagnosticlabs.com> D=e: Wed, October 02, 2013 5:54 am To: "Bauer, Karen L." <[2]Bauer.Karen@mayo.edu>, "'histonet@lists.utsouthwest=n.edu'" <[3]histonet@lists.utsouthwestern.edu> Hello Karen, =A Back in the old days of doing IHC manually on mostly fresh, froze n tissue I would immediately place the slide with the frozen section into a=plin jar of acetone to fix it. Keep the coplin jar of acetone in the cr=stat. Leave it in the acetone for only 10 minutes. Try diluting the Pr=ease 2 - 1:1 to make it gentler so it doesn't "eat" the tissue. This te=nique worked well when I was doing ER/PR on frozen breast tumors. Not su= it will work with skin but it's worth a try. Barbara S. Tib= Histology Supervisor Accurate Diagnostic Labs South Pl=nfield, NJ [4]barbara.tibbs@accuratediagnosticlabs.com 732-839-3374 C=l: 610-809-6508 _____________________________________=_ From: [5]histonet-bounces@lists.utsouthwestern.edu <[6]histonet-bounces@lists.utsouthwestern=du> on behalf of Bauer, Karen L. <[7]Bauer.Karen@mayo.edu> Sent: Tuesday, October 01, 20 5:22 PM To: '[8]=stonet@lists.utsouthwestern.edu' Subject: [Histonet] MOHS IPs Hi all, We are in the process of validating some a=ibodies in our MOHS lab. The Melan A (Mart 1) antibody is working well, =t it could be darker. We will be increasing the Ab incubation time to se=f that will help. As for the Pan Keratin, we cannot get it = work at all. We use Protease 2 on our Ultra stainer for FFPE tissues in=stology, but this stain in MOHS is placed on fresh, unfixed tissue... by=anual drop method. Any time we've tried to use an enzyme retrieval, th= tissue looks eaten up... even if we incubate if for a minute. =ASince the tissue is not fixed, I figured that no retrieval step would be=eded, but the Pan Keratin refuses to work with or without retrieval. =A For those of you in MOHS labs that are using a manual staining me thod for Melan A and Pan Keratin, would you be willing to share your protoc= with us? Thanks so much!! Karen K=en L. Bauer HTL/HT (ASCP) | Histology Supervisor | Pathology | MOHS Lab S=ervisor | Dermatology | Phone: 715-838-3205 | [9]bauer.karen@mayo.edu<[10]mailto:bauer.karen@mayo.edu> | Mayo Clinic Health System | 122=hipple Street | Eau Claire, WI 54702 | [11]mayoclinichealthsystem.org =A _______________________________________________ Histonet ma=ing list [13]Histo=t@lists.utsouthwestern.edu [14]http://lists.utsouthwestern.edu/mailman/l=tinfo/histonet _________________________________________=____ Histonet mailing list [15]Histonet@lists.utsouthwestern.edu [16]http://lists.utso=hwestern.edu/mailman/listinfo/histonet References 1. 3D"mailto:barbara.tibbs@accurated 2. ="mailto:Bauer.Karen@mayo.edu" 3. 3D"mailto:histonet@lists.utsouthwestern.edu 4. 3D"mailto:barbara.tibbs@accuratediagnosticlabs.c 5. 3D"mailto:histonet-bounces@lists.utsouthwestern.edu 6. 3D"mailto:histo 7. 3D"mailto:Bauer.Karen 8. 3D"mailto:histonet@lists.utsouthwestern.edu" 9. 3D"mailto:bauer.kar 10. 3D"mailto:bauer.karen@mayo 11. 3D"http://mayoclinichealt= 12. 3D"http:/ 13. 3D"mailto:Histonet@lists.utsouthwestern.edu" 14. 3D"http://lists.utsouthweste= 15. 3D"mailto:Histonet@lists.u 16. file://localhost/tmp/3D"h_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Sarah.Lewis <@t> nationwidechildrens.org Wed Oct 2 10:15:26 2013 From: Sarah.Lewis <@t> nationwidechildrens.org (Lewis, Sarah) Date: Wed Oct 2 10:15:42 2013 Subject: [Histonet] thawing and refreezing muscle? In-Reply-To: <524BE427.411C.0039.0@health.qld.gov.au> References: <761E2B5697F795489C8710BCC72141FF09BE2F@ex07.net.ucsf.edu> <524BE427.411C.0039.0@health.qld.gov.au> Message-ID: <4BAB3E4AF573804EA9929EFD1E158171243A605F@RESEXCHMBX01.CRII.ORG> I have tried this method as well and it works just as described below. Sarah E. Lewis HT, ASCP The Research Institute at Nationwide Children's Hospital Center for Gene Therapy Neuromuscular Division Rm WA3110 (614)722-2204 -----Original Message----- From: Tony Reilly [mailto:Tony_Reilly@health.qld.gov.au] Sent: Tuesday, October 01, 2013 7:15 PM To: Histonet; Timothy Morken Cc: jmitchell@neurology.wisc.edu Subject: Re: [Histonet] thawing and refreezing muscle? Hi Tim A guy in our histology group gave a presentation debunking the theory that ice crystal artefact is irreversible. His lab receives frozen specimens from thousands of kilometres away from labs with no histology staff. If there was ice crystal artefact he defrosts the specimen at room temperature. Mop the specimen with tissues to remove as much OCT and moisture as possible. Allow the specimen to remain at room temperature on the bench for 20-30 minutes depending on the size of the specimen to allow evaporation of more moisture. Refreeze the specimen with OCT and cut sections. The result was absence of ice crystal artefact but some separation of muscle bundles which does not interfere with staining. It appears the ice crystals do not damage the fibres just cause separation. So removing the moisture eliminates the problem. If you have a lot of muscle perhaps you could separate a small amount to trial the procedure. regards Tony Tony Reilly B.App.Sc. , M.Sc. Chief Scientist, Anatomical Pathology Pathology Queensland-PA Laboratory ________________________________________________ Health Services Support Agency | Department of Health Level 1, Building 15,Princess Alexandra Hospital Ipswich Road,WOOLLOONGABBA Qld4102 Ph: 07 3176 2412 Mob: 0402 139411 Fax: 07 3176 2930 Email: tony_reilly@health.qld.gov.au Web: www.health.qld.gov.au/qhcss/ >>> "Morken, Timothy" 10/2/2013 2:07 am >>> >>> Histonetters, Does anyone have a good method for thawing muscle and refreezing for histochemistry? With good results? We have some bulk-frozen muscle (centimeter thick) and that is what they want to try. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ********************************************************************************** From TJohnson <@t> gnf.org Wed Oct 2 10:23:30 2013 From: TJohnson <@t> gnf.org (Teri Johnson) Date: Wed Oct 2 10:23:43 2013 Subject: [Histonet] Re: MOHS IPs Message-ID: <9F3CFEE76E51B64991C7485270890B40497D0BC5@EX5.lj.gnf.org> Hi Karen, It could be that your cytokeratin antibody only recognizes a formalin fixed epitope conformation and enzyme digestion has nothing to do with it. Also try fixing in formalin and then try the stain again with and without the diluted enzyme digestion. Brief antigen retrieval (Citrate buffer pH 6.0, 30 minutes @ 60 degrees C) may also help. We have had good luck on minimally fixed frozen section staining using the lower temp AR with some antibodies. Intermediate filaments are best preserved with alcohol-based fixatives. You could also try using 95% ethanol fixative for 5 minutes prior to staining, rinse with buffer and proceed with staining, no digestion. Teri Johnson Manager, Histology Genomics Institute for Novartis Research Foundation San Diego, CA 858-332-4752 From smmiller <@t> comhs.org Wed Oct 2 11:10:25 2013 From: smmiller <@t> comhs.org (Sheila Miller) Date: Wed Oct 2 11:10:46 2013 Subject: [Histonet] Shipping to Countries Outside of the US Message-ID: <2711213F2132AB4F9DDC2F1A40F67FC5222A74DCE3@CFNIEXCH02.comhs.org> Hello All, A question was posed to me by our Cancer Resource Department... I am wondering if you/lab has had experience with shipping tissue specimens outside the country. We have a study where we may be doing this and they are asking if we have to have a special permit to ship outside our country. I believe it is going to Germany. Thank you, Sheila ____________________________________ This message and attachment(s), if any, is intended for the sole use of the individual and/or entity of which it is addressed, and may contain information that is privileged,confidential and prohibited from disclosure under applicable law. If you are not the addressee, or authorized to receive this on behalf of the addressee, you are hereby notified that you may not use, copy, disclose or distribute to anyone this message or any part thereof. If you have received this in error, please immediately advise the sender by e-mail and delete this information and all attachments from your computer and network. Thank you. ____________________________________ From turkekul <@t> gmail.com Wed Oct 2 12:11:22 2013 From: turkekul <@t> gmail.com (Mesru T) Date: Wed Oct 2 12:11:26 2013 Subject: [Histonet] Gram stain Message-ID: Hi All, Does anybody have a protocol for Gram stain on FFPE mouse intestine sections. We are looking to distinguish between Klebsiella and Vancomycin Resistant Enterococcus (VRE). I would like to avoid using Picric acid as some protocols suggest. Thanks in advance. From gu.lang <@t> gmx.at Wed Oct 2 12:38:56 2013 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Wed Oct 2 12:40:44 2013 Subject: [Histonet] Benchmark Ultra troubles Message-ID: <001a01cebf96$45a20a10$d0e61e30$@gmx.at> Hi Ventana-users, we have a special technical problem with our Ultra. It seems, that the liquid in the waste-tube is sometime pressed out of the hole on the platform like a fountain. You can imagine the nice surprise, when the oil drops from the Ultra-roof onto the carousel-top. Everything is oily. The technical service has'nt found a cause until now. The filter in the tube has been changed. Has anyone seen a similar problem? Solved the problem? Gudrun Lang From one_angel_secret <@t> yahoo.com Wed Oct 2 12:47:22 2013 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Wed Oct 2 12:47:38 2013 Subject: [Histonet] RE: MOHS IPs In-Reply-To: <8A2A8E67E35BB24DAD9375F9E16310750E3549@MSGPEXCHA01A.mfad.mfroot.org> References: <20131002073533.f86bd30e73b823f57b516b5451216a98.951d862201.wbe@email01.secureserver.net> <8A2A8E67E35BB24DAD9375F9E16310750E3549@MSGPEXCHA01A.mfad.mfroot.org> Message-ID: <628C0E60-8664-4DBC-B558-F3B5D9FEABF8@yahoo.com> What detection kit are you using? Sent from my iPhone On Oct 2, 2013, at 10:47 AM, "Bauer, Karen L." wrote: > Thanks for the reply, > > We are pre-fixing in a formalin substitute, but we'll give the acetone a try. I have diluted the protease, but have not gotten any good results. We'll keep trying with that as well. > > We've tried multiple incubation times for the cytokeratin... From 5 minutes all the way up to 30 minutes... With no luck. > > We are using an enhanced polymer DAB. > > Thanks for the suggestions! I greatly appreciate it! :) > > Karen > > Karen L. Bauer HTL/HT (ASCP) | Histology Supervisor | Pathology | MOHS Lab Supervisor | Dermatology | Phone: 715-838-3205 | bauer.karen@mayo.edu | Mayo Clinic Health System | 1221 Whipple Street | Eau Claire, WI 54702 | mayoclinichealthsystem.org > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of pruegg@ihctech.net > Sent: Wednesday, October 02, 2013 9:36 AM > To: Barbara Tibbs; Bauer, Karen L.; 'histonet@lists.utsouthwestern.edu' > Subject: RE: [Histonet] RE: MOHS IPs > > > I agree with Barbara try fixing in cold acetone/ethanol mix even = > just for a couple of minutes then go directly to buffer do not dry > after=x, I would dilute the protease 1:4 with buffer (PBS or TBS > what ever u u=) and do it for a short time. Enzyme digestion on > frozen sections i=ricky but fixing a little and diluting the > enzyme should help prevent ov= digestion. How long are u > incubating the primary? Are u usin=n enhanced labeled polymer > detection? HRP/Dab or AEC or Alk.P/fast=d? > > > > > > > > > -------- Original Message -------- > > Subject: [Histonet] RE: MOHS =s > > From: Barbara Tibbs <[1]barbara.tibbs@accuratediagnosticlabs.com> > > D=e: Wed, October 02, 2013 5:54 am > > To: "Bauer, Karen L." <[2]Bauer.Karen@mayo.edu>, > > "'histonet@lists.utsouthwest=n.edu'" > > <[3]histonet@lists.utsouthwestern.edu> > > > > Hello Karen, > =A > > Back in the old days of doing IHC manually on mostly fresh, froze n tissue I would immediately place the slide with the frozen section > into a=plin jar of acetone to fix it. Keep the coplin jar of > acetone in the cr=stat. Leave it in the acetone for only 10 > minutes. Try diluting the Pr=ease 2 - 1:1 to make it gentler so it > doesn't "eat" the tissue. This te=nique worked well when I was > doing ER/PR on frozen breast tumors. Not su= it will work with skin > but it's worth a try. > > > > Barbara S. Tib= > > Histology Supervisor > > Accurate Diagnostic Labs > > South Pl=nfield, NJ > > [4]barbara.tibbs@accuratediagnosticlabs.com > > 732-839-3374 > > C=l: 610-809-6508 > > > > > > _____________________________________=_ > > From: [5]histonet-bounces@lists.utsouthwestern.edu > <[6]histonet-bounces@lists.utsouthwestern=du> on behalf of Bauer, > Karen L. <[7]Bauer.Karen@mayo.edu> > > Sent: Tuesday, October 01, 20 5:22 PM > > To: '[8]=stonet@lists.utsouthwestern.edu' > > Subject: [Histonet] MOHS IPs > > > Hi all, > > > > We are in the process of validating some a=ibodies in our MOHS > lab. The Melan A (Mart 1) antibody is working well, =t it could be > darker. We will be increasing the Ab incubation time to se=f that > will help. > > > > As for the Pan Keratin, we cannot get it = work at all. We use > Protease 2 on our Ultra stainer for FFPE tissues in=stology, but > this stain in MOHS is placed on fresh, unfixed tissue... by=anual > drop method. Any time we've tried to use an enzyme retrieval, th= > tissue looks eaten up... even if we incubate if for a minute. > > > =ASince the tissue is not fixed, I figured that no retrieval step > would be=eded, but the Pan Keratin refuses to work with or without > retrieval. > =A > > For those of you in MOHS labs that are using a manual staining me thod for Melan A and Pan Keratin, would you be willing to share your > protoc= with us? > > > > Thanks so much!! > > > > Karen > > > > K=en L. Bauer HTL/HT (ASCP) | Histology Supervisor | Pathology | > MOHS Lab S=ervisor | Dermatology | Phone: 715-838-3205 | > [9]bauer.karen@mayo.edu<[10]mailto:bauer.karen@mayo.edu> | Mayo Clinic > Health System | 122=hipple Street | Eau Claire, WI 54702 | > [11]mayoclinichealthsystem.org rg/> > > > =A > > _______________________________________________ > > Histonet ma=ing list > > [13]Histo=t@lists.utsouthwestern.edu > > [14]http://lists.utsouthwestern.edu/mailman/l=tinfo/histonet > > > > _________________________________________=____ > > Histonet mailing list > > [15]Histonet@lists.utsouthwestern.edu > > [16]http://lists.utso=hwestern.edu/mailman/listinfo/histonet > > > > > > > > References > > 1. 3D"mailto:barbara.tibbs@accurated 2. ="mailto:Bauer.Karen@mayo.edu" > 3. 3D"mailto:histonet@lists.utsouthwestern.edu 4. 3D"mailto:barbara.tibbs@accuratediagnosticlabs.c 5. 3D"mailto:histonet-bounces@lists.utsouthwestern.edu 6. 3D"mailto:histo 7. 3D"mailto:Bauer.Karen 8. 3D"mailto:histonet@lists.utsouthwestern.edu" > 9. 3D"mailto:bauer.kar 10. 3D"mailto:bauer.karen@mayo 11. 3D"http://mayoclinichealt= > 12. 3D"http:/ 13. 3D"mailto:Histonet@lists.utsouthwestern.edu" > 14. 3D"http://lists.utsouthweste= > 15. 3D"mailto:Histonet@lists.u 16. file://localhost/tmp/3D"h_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From one_angel_secret <@t> yahoo.com Wed Oct 2 12:51:59 2013 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Wed Oct 2 12:52:14 2013 Subject: [Histonet] IHC turnaround time In-Reply-To: References: Message-ID: <75CAA15B-C45D-443E-87E9-E76143144E69@yahoo.com> In less than 8 hours I can get 96 Ihc stains done with our dako link and that is two runs Sent from my iPhone On Oct 1, 2013, at 1:15 PM, "McKenzie, Emily" wrote: > Hello all, > I am currently working on a Six Sigma Green Belt project and am looking for some data. Can any one give me their general turnaround times for IHC, from time of order to time of delivery? > Thanks for all your help with this, > > Emily K. McKenzie BS, HT(ASCP) > > Memorial Medical Center?701 North First Street?Springfield, IL 62781 > Ph: 217-788-3991?email: McKenzie.Emily@mhsil.com > > > > > ________________________________ > This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Wed Oct 2 13:15:17 2013 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Wed Oct 2 13:17:30 2013 Subject: [Histonet] Gram stain In-Reply-To: References: Message-ID: We have had great success with the Twort gram stain. From: Mesru T To: histonet@lists.utsouthwestern.edu Date: 10/02/2013 10:13 AM Subject: [Histonet] Gram stain Sent by: histonet-bounces@lists.utsouthwestern.edu Hi All, Does anybody have a protocol for Gram stain on FFPE mouse intestine sections. We are looking to distinguish between Klebsiella and Vancomycin Resistant Enterococcus (VRE). I would like to avoid using Picric acid as some protocols suggest. Thanks in advance. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From deganh <@t> upstate.edu Wed Oct 2 13:39:26 2013 From: deganh <@t> upstate.edu (Helene Degan) Date: Wed Oct 2 13:39:32 2013 Subject: [Histonet] Hi Message-ID: <524C301E0200007A000271AE@gatedom1.upstate.edu> Hi Im looking for an acid phosphatase enzyme procedure that might be out there the one we tried in the past was difficult to work up and get good reproducible results. Thanks Helene D Upstate University Medical Syracuse, NY From Robert-Eytalis <@t> RiversideHealthCare.net Wed Oct 2 13:40:31 2013 From: Robert-Eytalis <@t> RiversideHealthCare.net (Eytalis, Robert A) Date: Wed Oct 2 13:41:36 2013 Subject: [Histonet] RE: Validating new Benchmark instrument In-Reply-To: <0111BC10D77DC54EAB99B2DDA3BCE4B95A59BB@EXCH-2K10.hrhs.com> References: <0111BC10D77DC54EAB99B2DDA3BCE4B95A59BB@EXCH-2K10.hrhs.com> Message-ID: I am thinking there has to be a way to integrate the Validation into the Optimization the vendor does. I think you should be able to make microarray blocks/slides of at least 10 positive and more for ERPR per CAP during the optimization. Now, the vendor will say they only do Optimization, but there this is a way to get them to do the stains for you at least. You can create your own document for the Validation. When your manager negotiates the cost of the analyzer, see if the vendor can help out with the initial expense with an allowance. It sounds like you might be past this point. Has anyone else done it this way? Robert A. Eytalis Laboratory Manager robert-eytalis@riversidehealthcare.net Phone: (815) 935-7256 ext. 5186 (815) 935-7535 Fax (815) 935-7068 Riverside Medical Center 350 N. Wall Street - Kankakee, IL 60901 http://mail.riversidehealthcare.net/owa/redir.aspx?C=qIzWpCGMNkq2SuiIt1v_2X7GmC1aOtAI7VlOiEQ974hhCQzwyDH6yJklmjoBIK92OWDFwivhIZs.&URL=http%3a%2f%2fwww.riversidemc.net%2f | http://mail.riversidehealthcare.net/owa/redir.aspx?C=qIzWpCGMNkq2SuiIt1v_2X7GmC1aOtAI7VlOiEQ974hhCQzwyDH6yJklmjoBIK92OWDFwivhIZs.&URL=http%3a%2f%2fwww.facebook.com%2fRiversideMC ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Fawn Bomar [Fawn.Bomar@HalifaxRegional.com] Sent: Tuesday, October 01, 2013 12:06 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Validating new Benchmark instrument Hi everyone, I know that this subject has come up several times and I am sorry to bother everyone again but I was hoping to find some help. We are upgrading our Benchmark Immuno machines to the Benchmark XT immuno machine at the end of October. Does anyone have any suggestions on how to approach the validation process? Do we need to validate every antibody that we use as well as the kits? I know that there is also a gray area surrounding how many slides need to be run to consider the antibody validated, but what would be the suggested amount of slides to validate a new machine as I know this will be very costly. Does anybody have a log/checklist/spreadsheet that they would be willing to share to help document this validation of the machine and each of the antibodies? Thank you in advance for your help Fawn Bomar ------------------------------------------------------------- This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From plott <@t> uab.edu Wed Oct 2 13:51:22 2013 From: plott <@t> uab.edu (Patricia F Lott) Date: Wed Oct 2 13:51:30 2013 Subject: [Histonet] need lever clamp for blade of leica microtome Message-ID: <372C4AF089B6AE48B36F064FA891FF781380924D@UABEXMB3.ad.uab.edu> Does anyone have an extra lever clamp for a Leica microtome? I'd be happy to pay for it, and of course, for shipping! Thanks, Patty Lott UAB CMBD Core Lab 205-934-2007 From shive003 <@t> umn.edu Wed Oct 2 14:03:38 2013 From: shive003 <@t> umn.edu (Jan Shivers) Date: Wed Oct 2 14:03:44 2013 Subject: [Histonet] Shipping to Countries Outside of the US In-Reply-To: <2711213F2132AB4F9DDC2F1A40F67FC5222A74DCE3@CFNIEXCH02.comhs.org> References: <2711213F2132AB4F9DDC2F1A40F67FC5222A74DCE3@CFNIEXCH02.comhs.org> Message-ID: According to our Receiving department, each country/institution has its own permit requirements for receiving samples from outside of their own country. It is up to the shipper to inquire of the receiver as to what permits are needed to ship to that country (Germany, in your case). My source also said that FedEx would also have good information on this, since they ship nearly everywhere around the globe. Jan Shivers On Wed, Oct 2, 2013 at 11:10 AM, Sheila Miller wrote: > Hello All, > > A question was posed to me by our Cancer Resource Department... > > I am wondering if you/lab has had experience with shipping tissue > specimens outside the country. We have a study where we may be doing this > and they are asking if we have to have a special permit to ship outside our > country. I believe it is going to Germany. > > Thank you, > Sheila > > > ____________________________________ > > This message and attachment(s), if any, is intended for the sole use of > the individual and/or entity > of which it is addressed, and may contain information that is > privileged,confidential and prohibited > from disclosure under applicable law. If you are not the addressee, or > authorized to receive this on > behalf of the addressee, you are hereby notified that you may not use, > copy, disclose or distribute > to anyone this message or any part thereof. If you have received this in > error, please immediately advise > the sender by e-mail and delete this information and all attachments from > your computer and network. > Thank you. > ____________________________________ > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From yliang <@t> numirabio.com Wed Oct 2 14:24:21 2013 From: yliang <@t> numirabio.com (Yan Liang) Date: Wed Oct 2 14:24:26 2013 Subject: [Histonet] RE: Histotechnologist, (ASCP, QIHC certified) and Pathology assistant position in Seattle area In-Reply-To: References: Message-ID: <8DC126BD0D840947A161BBF46AB31688035AE3305C@DFW1MBX22.mex07a.mlsrvr.com> Needing a Histotechnologist (ASCP, QIHC certified) or Scientist (Histopathology) for a Seattle area. Five years of experience in routine histology including special stains, IHC and pathology knowledge. Send an updated resume to yliang@numirabio.com to be considered. A full job description is on the Numirabio.com .............................................. Yan Liang ?Ph.D. Senior Director, Histopathology Numira Biosciences Inc. 2023 120th Ave NE Bellevue, WA 98005 Phone: 425-777-4950 ext 201 Cell: 425-830-3090 Fax: 425-777-4949 yliang@numirabio.com www.numirabio.com www.altaportal.numirabio.com From TJohnson <@t> gnf.org Wed Oct 2 17:57:22 2013 From: TJohnson <@t> gnf.org (Teri Johnson) Date: Wed Oct 2 17:57:30 2013 Subject: [Histonet] Vacuum and pressure in tissue processing Message-ID: <9F3CFEE76E51B64991C7485270890B40497D12D6@EX5.lj.gnf.org> Dear friends, I recall hearing at a conference (or maybe it was just a casual conversation by an expert during a NSH symposium break) that vacuum and pressure in tissue processing really accomplishes very little. I do believe that using heat and agitation of the solutions provides more activity kinetically and therefore makes processing more efficient. Can someone affirm or deny the efficacy of vacuum and/or pressure in tissue processing, please? Thank you, as always, for your wisdom. Teri Johnson Manager, Histology Genomics Institute for Novartis Research Foundation San Diego, CA 858-332-4752 From geralddavydov <@t> gmail.com Wed Oct 2 20:04:01 2013 From: geralddavydov <@t> gmail.com (Gerald Davydov) Date: Wed Oct 2 20:04:05 2013 Subject: [Histonet] Trivew Message-ID: Please share protocol for Triview from Zetta Corporation for Venatna. From PEROWES10 <@t> juniata.edu Wed Oct 2 23:32:48 2013 From: PEROWES10 <@t> juniata.edu (Perow, Elliott S (PEROWES10)) Date: Wed Oct 2 23:32:58 2013 Subject: [Histonet] Inconsistent Sections with Cryostat Message-ID: Hello All, I have a Leica Cryocut 1800 with a Reichert-Jung 2020 microtome that has sat for 8 years and went through a move from one academic building to another. I am trying to section brook trout brain, but am having difficulty getting consistent sections. I will get a good section and then the next section will be a very very thin shaving of usually just the top portion of the OCT block. I have tried adjusting the clearance angle, made sure the blade and specimen is secured, replaced the blade with a new one, have tried different sectioning speeds, different thicknesses of the section and still continually only get a decent section every other turn. I was wondering if anyone has had any similar experiences or if maybe the machine just needs a maintenance check due to it being an older machine. I wasn't sure if the advancement mechanism may be off or if it is another issue that doesn't seem apparent to me. Any help would be greatly appreciated. Thanks, Elliott Perow Juniata College Biology POE CONFIDENTIALITY NOTICE: The materials in this electronic mail transmission (including all attachments) are private and confidential and are the property of the sender. The information contained in the material is privileged and is intended only for the use of the named addressee(s). If you are not the intended addressee, be advised that any unauthorized disclosure, copying, distribution or the taking of any action in reliance on the contents of this material is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender by replying to the e-mail, and then destroy it immediately. Thank you. From SStephenson <@t> lifecell.com Thu Oct 3 06:21:14 2013 From: SStephenson <@t> lifecell.com (Stephenson, Sheryl) Date: Thu Oct 3 06:21:21 2013 Subject: [Histonet] HT HistoDeck question... In-Reply-To: <9F3CFEE76E51B64991C7485270890B40497D12D6@EX5.lj.gnf.org> References: <9F3CFEE76E51B64991C7485270890B40497D12D6@EX5.lj.gnf.org> Message-ID: Hi, Please clarify why this answer to the HistoDeck study question is a) and not b). Here is the question: 'Frozen section slides cut from fresh, unfixed tissue specimens are optimally fixed in which of the following solutions? a) 37%-40% formaldehyde b) Cold acetone c) Acetic acid alcohol d) Alcoholic formalin Thanks, Sheryl Stephenson | Histology Technician _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From philip_manfre <@t> merck.com Thu Oct 3 06:54:56 2013 From: philip_manfre <@t> merck.com (Manfre, Philip) Date: Thu Oct 3 06:55:07 2013 Subject: [Histonet] RE: Inconsistent Sections with Cryostat In-Reply-To: References: Message-ID: <558A4571351D0C42BD923F403F4198C4B27103726B@USCTMXP51014.merck.com> I believe you may need to have the unit serviced. It sounds like something is not tight enough, perhaps the stage or blade holder unit. You said you secured everything which makes me think you have some issue with the cryostat itself. If a sharp blade, tightened blade and specimen, and varying the speed doesn't work, then you may need professional assistance form a service technician, especially if it is an older unit that has been moved around. Have you tried different temperatures? I don't think that is the problem but it is worth a try. Good luck! Cryosectioning can be an art in itself. Those decent sections are likely thicker than you intend, by the way. Classic "thick and thin". Philip Manfre, B.A., HT (ASCP) Associate Principal Scientist Merck Research Laboratories WP45-251 PO Box 4 West Point, PA 19486 215-652-9750 215-993-0383 (fax) philip_manfre@merck.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Perow, Elliott S (PEROWES10) Sent: Thursday, October 03, 2013 12:33 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Inconsistent Sections with Cryostat Hello All, I have a Leica Cryocut 1800 with a Reichert-Jung 2020 microtome that has sat for 8 years and went through a move from one academic building to another. I am trying to section brook trout brain, but am having difficulty getting consistent sections. I will get a good section and then the next section will be a very very thin shaving of usually just the top portion of the OCT block. I have tried adjusting the clearance angle, made sure the blade and specimen is secured, replaced the blade with a new one, have tried different sectioning speeds, different thicknesses of the section and still continually only get a decent section every other turn. I was wondering if anyone has had any similar experiences or if maybe the machine just needs a maintenance check due to it being an older machine. I wasn't sure if the advancement mechanism may be off or if it is another issue that doesn't seem apparent to me. Any help would be greatly appreciated. Thanks, Elliott Perow Juniata College Biology POE CONFIDENTIALITY NOTICE: The materials in this electronic mail transmission (including all attachments) are private and confidential and are the property of the sender. The information contained in the material is privileged and is intended only for the use of the named addressee(s). If you are not the intended addressee, be advised that any unauthorized disclosure, copying, distribution or the taking of any action in reliance on the contents of this material is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender by replying to the e-mail, and then destroy it immediately. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From lpwenk <@t> sbcglobal.net Thu Oct 3 07:15:56 2013 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Thu Oct 3 07:16:02 2013 Subject: [Histonet] HT HistoDeck question... In-Reply-To: References: <9F3CFEE76E51B64991C7485270890B40497D12D6@EX5.lj.gnf.org> Message-ID: <8FAB0141C65B42098CE4F110ECE9F372@HP2010> Personally, I think it's "a" is a wrong answer, and that you are correct that "b" is a better answer. My students and I have found a couple of other questions that we thought had the wrong answer indicated in the study set. Peggy A. Wenk, HTL(ASCP)SLS -----Original Message----- From: Stephenson, Sheryl Sent: Thursday, October 03, 2013 7:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HT HistoDeck question... Hi, Please clarify why this answer to the HistoDeck study question is a) and not b). Here is the question: 'Frozen section slides cut from fresh, unfixed tissue specimens are optimally fixed in which of the following solutions? a) 37%-40% formaldehyde b) Cold acetone c) Acetic acid alcohol d) Alcoholic formalin Thanks, Sheryl Stephenson | Histology Technician _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpwenk <@t> sbcglobal.net Thu Oct 3 07:21:55 2013 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Thu Oct 3 07:22:01 2013 Subject: [Histonet] RE: Inconsistent Sections with Cryostat In-Reply-To: <558A4571351D0C42BD923F403F4198C4B27103726B@USCTMXP51014.merck.com> References: <558A4571351D0C42BD923F403F4198C4B27103726B@USCTMXP51014.merck.com> Message-ID: I just attended Jan Minshew's workshop on cryostats at the NSH Symposium in Providence, RI, and she brought up something I had never thought of that causes thick and thin. If the handle that tightens the blade in the blade holder is over-tightened, the blade will become bowed, and that will cause thick-and-thin during sectioning. According to Jan, the handle should be tightened to be parallel to the angle of the blade holder "slope". A lot of times, the handle can be pushed further back towards the back of the cryostat, beyond being parallel with the slope. The thought seems to be, if tight is good, then tighter is better, but not in this case. Peggy A. Wenk, HTL(ASCP)SLS -----Original Message----- From: Manfre, Philip Sent: Thursday, October 03, 2013 7:54 AM To: Perow, Elliott S (PEROWES10) ; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Inconsistent Sections with Cryostat I believe you may need to have the unit serviced. It sounds like something is not tight enough, perhaps the stage or blade holder unit. You said you secured everything which makes me think you have some issue with the cryostat itself. If a sharp blade, tightened blade and specimen, and varying the speed doesn't work, then you may need professional assistance form a service technician, especially if it is an older unit that has been moved around. Have you tried different temperatures? I don't think that is the problem but it is worth a try. Good luck! Cryosectioning can be an art in itself. Those decent sections are likely thicker than you intend, by the way. Classic "thick and thin". Philip Manfre, B.A., HT (ASCP) Associate Principal Scientist Merck Research Laboratories WP45-251 PO Box 4 West Point, PA 19486 215-652-9750 215-993-0383 (fax) philip_manfre@merck.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Perow, Elliott S (PEROWES10) Sent: Thursday, October 03, 2013 12:33 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Inconsistent Sections with Cryostat Hello All, I have a Leica Cryocut 1800 with a Reichert-Jung 2020 microtome that has sat for 8 years and went through a move from one academic building to another. I am trying to section brook trout brain, but am having difficulty getting consistent sections. I will get a good section and then the next section will be a very very thin shaving of usually just the top portion of the OCT block. I have tried adjusting the clearance angle, made sure the blade and specimen is secured, replaced the blade with a new one, have tried different sectioning speeds, different thicknesses of the section and still continually only get a decent section every other turn. I was wondering if anyone has had any similar experiences or if maybe the machine just needs a maintenance check due to it being an older machine. I wasn't sure if the advancement mechanism may be off or if it is another issue that doesn't seem apparent to me. Any help would be greatly appreciated. Thanks, Elliott Perow Juniata College Biology POE CONFIDENTIALITY NOTICE: The materials in this electronic mail transmission (including all attachments) are private and confidential and are the property of the sender. The information contained in the material is privileged and is intended only for the use of the named addressee(s). If you are not the intended addressee, be advised that any unauthorized disclosure, copying, distribution or the taking of any action in reliance on the contents of this material is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender by replying to the e-mail, and then destroy it immediately. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Linda.Watson <@t> bms.com Thu Oct 3 08:40:06 2013 From: Linda.Watson <@t> bms.com (Watson, Linda) Date: Thu Oct 3 08:40:13 2013 Subject: [Histonet] HT HistoDeck question... In-Reply-To: <8FAB0141C65B42098CE4F110ECE9F372@HP2010> References: <9F3CFEE76E51B64991C7485270890B40497D12D6@EX5.lj.gnf.org> <8FAB0141C65B42098CE4F110ECE9F372@HP2010> Message-ID: <351A66CE7FB11D40AB8AC8FE5559EC7E11A975A411@ushpwbmsmmp008.one.ads.bms.com> For frozen cut sections, would the fixation also depend on what you plan on doing with it. For example, H&E, Special Stain or IHC? Please correct if I am wrong. I think that is a trick question!!! >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet- >bounces@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk >Sent: Thursday, October 03, 2013 8:16 AM >To: Stephenson, Sheryl; histonet@lists.utsouthwestern.edu >Subject: Re: [Histonet] HT HistoDeck question... > >Personally, I think it's "a" is a wrong answer, and that you are correct >that "b" is a better answer. My students and I have found a couple of >other >questions that we thought had the wrong answer indicated in the study >set. > >Peggy A. Wenk, HTL(ASCP)SLS >-----Original Message----- >From: Stephenson, Sheryl >Sent: Thursday, October 03, 2013 7:21 AM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] HT HistoDeck question... > >Hi, >Please clarify why this answer to the HistoDeck study question is a) >and >not b). > >Here is the question: > > 'Frozen section slides cut from fresh, unfixed tissue specimens are >optimally fixed in which of the following solutions? >a) 37%-40% formaldehyde >b) Cold acetone >c) Acetic acid alcohol >d) Alcoholic formalin > >Thanks, > > >Sheryl Stephenson | Histology Technician > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. From b-frederick <@t> northwestern.edu Thu Oct 3 08:42:34 2013 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Thu Oct 3 08:42:39 2013 Subject: [Histonet] HT HistoDeck question... In-Reply-To: <351A66CE7FB11D40AB8AC8FE5559EC7E11A975A411@ushpwbmsmmp008.one.ads.bms.com> References: <9F3CFEE76E51B64991C7485270890B40497D12D6@EX5.lj.gnf.org> <8FAB0141C65B42098CE4F110ECE9F372@HP2010> <351A66CE7FB11D40AB8AC8FE5559EC7E11A975A411@ushpwbmsmmp008.one.ads.bms.com> Message-ID: <62C639732D3F274DACED033EBDF6ADAF2F00D4EA@evcspmbx2.ads.northwestern.edu> We fix H&E's in 95% and our IHC protocol is acetone/alcohol fixation. Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Watson, Linda Sent: Thursday, October 03, 2013 8:40 AM To: Lee & Peggy Wenk; Stephenson, Sheryl; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HT HistoDeck question... For frozen cut sections, would the fixation also depend on what you plan on doing with it. For example, H&E, Special Stain or IHC? Please correct if I am wrong. I think that is a trick question!!! >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet- >bounces@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk >Sent: Thursday, October 03, 2013 8:16 AM >To: Stephenson, Sheryl; histonet@lists.utsouthwestern.edu >Subject: Re: [Histonet] HT HistoDeck question... > >Personally, I think it's "a" is a wrong answer, and that you are >correct that "b" is a better answer. My students and I have found a >couple of other questions that we thought had the wrong answer >indicated in the study set. > >Peggy A. Wenk, HTL(ASCP)SLS >-----Original Message----- >From: Stephenson, Sheryl >Sent: Thursday, October 03, 2013 7:21 AM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] HT HistoDeck question... > >Hi, >Please clarify why this answer to the HistoDeck study question is a) >and not b). > >Here is the question: > > 'Frozen section slides cut from fresh, unfixed tissue specimens are >optimally fixed in which of the following solutions? >a) 37%-40% formaldehyde >b) Cold acetone >c) Acetic acid alcohol >d) Alcoholic formalin > >Thanks, > > >Sheryl Stephenson | Histology Technician > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 <@t> earthlink.net Thu Oct 3 08:59:58 2013 From: relia1 <@t> earthlink.net (Pam Barker) Date: Thu Oct 3 09:00:10 2013 Subject: [Histonet] The NSH was AMAZING!!! Congratulations to the 2013 Leadership, Education and Advocacy Award Winners!! From Pam Barker and RELIA Solutions!! Message-ID: <006001cec040$d9e6e1f0$8db4a5d0$@earthlink.net> Hi Histonetters!!! How are you doing? The NSH was AMAZING!!!!! Providence was GORGEOUS!!!! The Speakers were BRILLIANT!!!! CONGRATULATIONS TO THE 2013 Leadership Education And Advocacy Award Winners: Histotechnologist of the Year-Wanda Jones J.B. McCormick Award- Peggy Wenk President's Award- Janet Dapson Lee G. Luna Foreign Travel Scholarship- M. Lamar Jones William Hacker Memorial Award- Anthony Wong Rosemary & Donald Ostermeier Memorial Award- Christiane Coady Ventana IHC Award- Tiana Baskin Biogenex Standardization in IHC Award-David Davis Biogenex Standardization in ISH Award Dale Telgenhoff Dako Standardization of IHC Award-Jennifer Wright Anne Preece Award -Sarah Mack Leica Leadership in Management Award-Nirmala Amin Leica Leadership in Teaching Award- Elaine Basham Newcomer Helping Hand Award- Jean Mitchell Epitomics IHC Award- Melissa Downing Jules Elias Excellence in IHC Award- James Burchette Since I have returned to the office my phone has been ringing off the hook with exciting new opportunities. Here is a list of my current openings. All of these are full time permanent positions. My clients offer excellent compensation, benefits and relocation assistance. And they are ready to interview and hire right away! HISTOLOGY SUPERVISORS/MANAGERS: Histology Supervisor - Tallahassee, FL Histology Supervisor - West Palm Beach, FL Histology Supervisor - Seattle, WA Lead Pathology Tech - East of Columbus, OH HISTOTECHNICIANS/HISTOTECHNOLOGISTS: Histology Tech with Electron Microscopy - Southern CA Histotechnician Day shift -East of Columbus, OH Histotechnician Day shift - Tyler, TX Histotechnologist - Days - Seattle, WA Histology Tech - West Palm Beach, FL Histotechnician - Days - Harrisonburg, VA Histotechnician - Nights - Nashville, TN Senior Histotechnologist with FISH - Louisville, KY OTHER OPPORTUNITIES MT (ASCP) with Molecular experience- RTP, SC Of course I can't put all the information about these opportunities in an e-mail. So if you or anyone you know might be interested in hearing more about any of these positions or want help with a job search in another area please contact me. I can be reached at 866-607-3542 or relia1@earthlink.net. Remember it never hurts to look. Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From SStephenson <@t> lifecell.com Thu Oct 3 09:02:56 2013 From: SStephenson <@t> lifecell.com (Stephenson, Sheryl) Date: Thu Oct 3 09:03:27 2013 Subject: [Histonet] HT HistoDeck question... In-Reply-To: <62C639732D3F274DACED033EBDF6ADAF2F00D4EA@evcspmbx2.ads.northwestern.edu> References: <9F3CFEE76E51B64991C7485270890B40497D12D6@EX5.lj.gnf.org> <8FAB0141C65B42098CE4F110ECE9F372@HP2010> <351A66CE7FB11D40AB8AC8FE5559EC7E11A975A411@ushpwbmsmmp008.one.ads.bms.com> <62C639732D3F274DACED033EBDF6ADAF2F00D4EA@evcspmbx2.ads.northwestern.edu> Message-ID: Thank you All! Sheryl Stephenson | Histology Technician ? -----Original Message----- From: Bernice Frederick [mailto:b-frederick@northwestern.edu] Sent: Thursday, October 03, 2013 9:43 AM To: Watson, Linda; Lee & Peggy Wenk; Stephenson, Sheryl; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HT HistoDeck question... We fix H&E's in 95% and our IHC protocol is acetone/alcohol fixation. Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Watson, Linda Sent: Thursday, October 03, 2013 8:40 AM To: Lee & Peggy Wenk; Stephenson, Sheryl; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HT HistoDeck question... For frozen cut sections, would the fixation also depend on what you plan on doing with it. For example, H&E, Special Stain or IHC? Please correct if I am wrong. I think that is a trick question!!! >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet- >bounces@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk >Sent: Thursday, October 03, 2013 8:16 AM >To: Stephenson, Sheryl; histonet@lists.utsouthwestern.edu >Subject: Re: [Histonet] HT HistoDeck question... > >Personally, I think it's "a" is a wrong answer, and that you are >correct that "b" is a better answer. My students and I have found a >couple of other questions that we thought had the wrong answer >indicated in the study set. > >Peggy A. Wenk, HTL(ASCP)SLS >-----Original Message----- >From: Stephenson, Sheryl >Sent: Thursday, October 03, 2013 7:21 AM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] HT HistoDeck question... > >Hi, >Please clarify why this answer to the HistoDeck study question is a) >and not b). > >Here is the question: > > 'Frozen section slides cut from fresh, unfixed tissue specimens are >optimally fixed in which of the following solutions? >a) 37%-40% formaldehyde >b) Cold acetone >c) Acetic acid alcohol >d) Alcoholic formalin > >Thanks, > > >Sheryl Stephenson | Histology Technician > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpwenk <@t> sbcglobal.net Thu Oct 3 09:21:42 2013 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Thu Oct 3 09:21:52 2013 Subject: [Histonet] HT HistoDeck question... In-Reply-To: <351A66CE7FB11D40AB8AC8FE5559EC7E11A975A411@ushpwbmsmmp008.one.ads.bms.com> References: <9F3CFEE76E51B64991C7485270890B40497D12D6@EX5.lj.gnf.org> <8FAB0141C65B42098CE4F110ECE9F372@HP2010> <351A66CE7FB11D40AB8AC8FE5559EC7E11A975A411@ushpwbmsmmp008.one.ads.bms.com> Message-ID: <335B9C2DCE414D1CB831DCCB07DEDC94@HP2010> I agree, there is probably more than one correct answer to this question, depending upon whether you are planning on doing stains for lipids, IHC, immunofluoresence or muscle enzymes. But I don't think (A) full strength 37-40% formaldehyde solution would ever be the correct answer. Unless you put a gauze in the bottom on the coplin jar, pour a little conc. formaldehyde on the gauze, put a lid on the coplin jar, and fix the section in formaldehyde vapors. But the question does say SOLUTION, not VAPOR. So I still think A is wrong. And most likely full strength acetic acid (C) is wrong - would eat the tissue off the slide. That leaves cold acetone (B) , which is good for some antibodies and some enzymes, or alcoholic formalin (D) which might be OK, but most of the time things either like alcohol and hate formalin, or they like formalin and don't like alcohol. So I would think most FS that we want to fix would not particularly like alcoholic formalin. And none of the solutions listed are good for lipids. So, given the question (with incomplete information) and the choices of answers, I would still side with (B) cold acetone. Now - a little aside - for the questions on the ASCP HT and HTL exams - if it is a new question, the people on the HT/HTL exam committee would be looking at it intensely before it goes on the exam for the first time. If the committee people are having problems answering it (like we are here), the question would be reworked until all the issues are resolved (such as putting in "for lymph node IHC" into the question). If it makes it past the committee, and the stats from the exam show that many people are having problems answering it, the question is pulled from the exam and is not used in the person's score. The question is then sent back to the HT/HTL exam committee, to try to figure out why examinees were having problems, and the question reworked again. As someone who has written exam questions at the school for 20+ years, I can tell you that it really is hard to write exam questions. I think I've covered everything in the question so that it is straight forward, and then half the students read something into the question that I never thought of, or come up with a written answer that I never considered. So I either have to throw out the question or give the point to the student, depending upon what's going on. And then mark the question for a re-write next year. And that doesn't include me marking the wrong answer on my master sheet. It happens! Peggy A. Wenk, HTL(ASCP)SLS -----Original Message----- From: Watson, Linda Sent: Thursday, October 03, 2013 9:40 AM To: Lee & Peggy Wenk ; Stephenson, Sheryl ; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HT HistoDeck question... For frozen cut sections, would the fixation also depend on what you plan on doing with it. For example, H&E, Special Stain or IHC? Please correct if I am wrong. I think that is a trick question!!! >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet- >bounces@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk >Sent: Thursday, October 03, 2013 8:16 AM >To: Stephenson, Sheryl; histonet@lists.utsouthwestern.edu >Subject: Re: [Histonet] HT HistoDeck question... > >Personally, I think it's "a" is a wrong answer, and that you are correct >that "b" is a better answer. My students and I have found a couple of >other >questions that we thought had the wrong answer indicated in the study >set. > >Peggy A. Wenk, HTL(ASCP)SLS >-----Original Message----- >From: Stephenson, Sheryl >Sent: Thursday, October 03, 2013 7:21 AM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] HT HistoDeck question... > >Hi, >Please clarify why this answer to the HistoDeck study question is a) >and >not b). > >Here is the question: > > 'Frozen section slides cut from fresh, unfixed tissue specimens are >optimally fixed in which of the following solutions? >a) 37%-40% formaldehyde >b) Cold acetone >c) Acetic acid alcohol >d) Alcoholic formalin > >Thanks, > > >Sheryl Stephenson | Histology Technician > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. From rjbuesa <@t> yahoo.com Thu Oct 3 09:29:48 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Oct 3 09:29:55 2013 Subject: [Histonet] Vacuum and pressure in tissue processing In-Reply-To: <9F3CFEE76E51B64991C7485270890B40497D12D6@EX5.lj.gnf.org> References: <9F3CFEE76E51B64991C7485270890B40497D12D6@EX5.lj.gnf.org> Message-ID: <1380810588.70746.YahooMailNeo@web163103.mail.bf1.yahoo.com> When tissue processing was manual there were some "gadgets" providing vacuum and those using it reported better results. The fact of the matter was that manual processing is so slow that anything you introduce will favor the process. Static tissue processors, i.e. those that only mover the specimens circumventing the manual transfer like the HistoKinete only improved processing if they were able to move the specimens, something they did by adding rotation inside the reagents vessels. Retort tissue processors introduced 3 novelties: vacuum, pressure and, most importantly, agitation that is nothing but empty/fill the whole retort every 20 minutes. This agitation is more important, as you point out, than the vacuum/pressure. Ren? J. ________________________________ From: Teri Johnson To: "histonet@lists.utsouthwestern.edu" Sent: Wednesday, October 2, 2013 6:57 PM Subject: [Histonet] Vacuum and pressure in tissue processing Dear friends, I recall hearing at a conference (or maybe it was just a casual conversation by an expert during a NSH symposium break) that vacuum and pressure in tissue processing really accomplishes very little. I do believe that using heat and agitation of the solutions provides more activity kinetically and therefore makes processing more efficient. Can someone affirm or deny the efficacy of vacuum and/or pressure in tissue processing, please? Thank you, as always, for your wisdom. Teri Johnson Manager, Histology Genomics Institute for Novartis Research Foundation San Diego, CA 858-332-4752 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From McKenzie.Emily <@t> mhsil.com Thu Oct 3 09:39:15 2013 From: McKenzie.Emily <@t> mhsil.com (McKenzie, Emily) Date: Thu Oct 3 09:39:23 2013 Subject: [Histonet] HT HistoDeck question... In-Reply-To: <335B9C2DCE414D1CB831DCCB07DEDC94@HP2010> References: <9F3CFEE76E51B64991C7485270890B40497D12D6@EX5.lj.gnf.org> <8FAB0141C65B42098CE4F110ECE9F372@HP2010> <351A66CE7FB11D40AB8AC8FE5559EC7E11A975A411@ushpwbmsmmp008.one.ads.bms.com> <335B9C2DCE414D1CB831DCCB07DEDC94@HP2010> Message-ID: Honestly I think this all depends on what they are actually asking. Are they asking what you fix the tissue in after the frozen is complete and you need to submit the remaining tissue for routine processing? If so, then A is the correct answer. Are they asking what you fix the tissue to the slide with to stain the frozen section for immediate diagnosis? If so, then the answer can be several possibilities depending on what you are going to be staining/ the process you will be staining with. I have worked in several different hospitals and each have a different protocol for there frozen section staining. In order to fix the tissue to the slide I have used 95% alcohol and acidic acid alcohol. The only time I have used formaldehyde is when I use the vapors for fat staining. I think this might be a question to take up with ASCP. They are the ones administering the test. Emily K. McKenzie BS, HT(ASCP) Memorial Medical Center?701 North First Street?Springfield, IL 62781 Ph: 217-788-3991?email: McKenzie.Emily@mhsil.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk Sent: Thursday, October 03, 2013 9:22 AM To: Watson, Linda; Stephenson, Sheryl; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] HT HistoDeck question... I agree, there is probably more than one correct answer to this question, depending upon whether you are planning on doing stains for lipids, IHC, immunofluoresence or muscle enzymes. But I don't think (A) full strength 37-40% formaldehyde solution would ever be the correct answer. Unless you put a gauze in the bottom on the coplin jar, pour a little conc. formaldehyde on the gauze, put a lid on the coplin jar, and fix the section in formaldehyde vapors. But the question does say SOLUTION, not VAPOR. So I still think A is wrong. And most likely full strength acetic acid (C) is wrong - would eat the tissue off the slide. That leaves cold acetone (B) , which is good for some antibodies and some enzymes, or alcoholic formalin (D) which might be OK, but most of the time things either like alcohol and hate formalin, or they like formalin and don't like alcohol. So I would think most FS that we want to fix would not particularly like alcoholic formalin. And none of the solutions listed are good for lipids. So, given the question (with incomplete information) and the choices of answers, I would still side with (B) cold acetone. Now - a little aside - for the questions on the ASCP HT and HTL exams - if it is a new question, the people on the HT/HTL exam committee would be looking at it intensely before it goes on the exam for the first time. If the committee people are having problems answering it (like we are here), the question would be reworked until all the issues are resolved (such as putting in "for lymph node IHC" into the question). If it makes it past the committee, and the stats from the exam show that many people are having problems answering it, the question is pulled from the exam and is not used in the person's score. The question is then sent back to the HT/HTL exam committee, to try to figure out why examinees were having problems, and the question reworked again. As someone who has written exam questions at the school for 20+ years, I can tell you that it really is hard to write exam questions. I think I've covered everything in the question so that it is straight forward, and then half the students read something into the question that I never thought of, or come up with a written answer that I never considered. So I either have to throw out the question or give the point to the student, depending upon what's going on. And then mark the question for a re-write next year. And that doesn't include me marking the wrong answer on my master sheet. It happens! Peggy A. Wenk, HTL(ASCP)SLS -----Original Message----- From: Watson, Linda Sent: Thursday, October 03, 2013 9:40 AM To: Lee & Peggy Wenk ; Stephenson, Sheryl ; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HT HistoDeck question... For frozen cut sections, would the fixation also depend on what you plan on doing with it. For example, H&E, Special Stain or IHC? Please correct if I am wrong. I think that is a trick question!!! >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet- >bounces@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk >Sent: Thursday, October 03, 2013 8:16 AM >To: Stephenson, Sheryl; histonet@lists.utsouthwestern.edu >Subject: Re: [Histonet] HT HistoDeck question... > >Personally, I think it's "a" is a wrong answer, and that you are >correct that "b" is a better answer. My students and I have found a >couple of other questions that we thought had the wrong answer >indicated in the study set. > >Peggy A. Wenk, HTL(ASCP)SLS >-----Original Message----- >From: Stephenson, Sheryl >Sent: Thursday, October 03, 2013 7:21 AM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] HT HistoDeck question... > >Hi, >Please clarify why this answer to the HistoDeck study question is a) >and not b). > >Here is the question: > > 'Frozen section slides cut from fresh, unfixed tissue specimens are >optimally fixed in which of the following solutions? >a) 37%-40% formaldehyde >b) Cold acetone >c) Acetic acid alcohol >d) Alcoholic formalin > >Thanks, > > >Sheryl Stephenson | Histology Technician > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From BGapinski <@t> pathgroup.com Thu Oct 3 09:46:43 2013 From: BGapinski <@t> pathgroup.com (Bruce Gapinski) Date: Thu Oct 3 09:46:48 2013 Subject: [Histonet] Microtomes Message-ID: Dear Histonians I'm in the market (please, no vendors) for a new microtome. I'd like an automated/manual type. What is on the market these days that is worthy? I know about Leica, but it's been many years since I've looked for these instruments and would love to know what you like and don't like about your microtomes? Respectfully, Bruce Gapinsk HT (ASCP) Chief Histologist Marin Medical Laboratories PathGroup SF ________________________________ Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you From algranth <@t> email.arizona.edu Thu Oct 3 10:30:26 2013 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Thu Oct 3 10:30:32 2013 Subject: [Histonet] HT HistoDeck question... In-Reply-To: References: <9F3CFEE76E51B64991C7485270890B40497D12D6@EX5.lj.gnf.org> <8FAB0141C65B42098CE4F110ECE9F372@HP2010> <351A66CE7FB11D40AB8AC8FE5559EC7E11A975A411@ushpwbmsmmp008.one.ads.bms.com> <335B9C2DCE414D1CB831DCCB07DEDC94@HP2010> Message-ID: I'd go with "A", but it really depends on what you are going to do with the sections after fixation. In the protocol for Oil Red O in Freida's second edition (that I use almost daily combined with some steps from PolyScientific's ORO protocol), step #1 says to fix in 40% formaldehyde. Doesn't say vapors so I plunk the slides in the liquid 40%. I have fixed frozen sections for regular H&E and other stains as well in 40% formaldehyde and they come out beautiful. Unless there is some specific request or reason to use cold acetone or alcohol this is what I use. Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 From rjbuesa <@t> yahoo.com Thu Oct 3 10:48:16 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Oct 3 10:48:22 2013 Subject: [Histonet] Microtomes In-Reply-To: References: Message-ID: <1380815296.64587.YahooMailNeo@web163103.mail.bf1.yahoo.com> Still Leica Ren? J. ________________________________ From: Bruce Gapinski To: "'histonet@lists.utsouthwestern.edu'" Sent: Thursday, October 3, 2013 10:46 AM Subject: [Histonet] Microtomes Dear Histonians ? ? ? ? ? ? ? ? I'm in the market (please, no vendors) for a new microtome. I'd like an automated/manual type. What is on the market these days that is worthy? I know about Leica, but it's been many years since I've looked for these instruments and would love to know what you like and don't like about your microtomes? Respectfully, Bruce Gapinsk HT (ASCP) Chief Histologist Marin Medical Laboratories PathGroup SF ________________________________ Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Thu Oct 3 10:58:42 2013 From: joelleweaver <@t> hotmail.com (=?utf-8?B?am9lbGxld2VhdmVyQGhvdG1haWwuY29t?=) Date: Thu Oct 3 10:58:53 2013 Subject: =?utf-8?B?UmU6IFtIaXN0b25ldF0gTWljcm90b21lcw==?= Message-ID: Still don't have use for fully automated. 1st microm , 2nd Leica Opinion only Sent from my Verizon Wireless 4G LTE Smartphone ----- Reply message ----- From: "Bruce Gapinski" To: "'histonet@lists.utsouthwestern.edu'" Subject: [Histonet] Microtomes Date: Thu, Oct 3, 2013 10:46 am Dear Histonians I'm in the market (please, no vendors) for a new microtome. I'd like an automated/manual type. What is on the market these days that is worthy? I know about Leica, but it's been many years since I've looked for these instruments and would love to know what you like and don't like about your microtomes? Respectfully, Bruce Gapinsk HT (ASCP) Chief Histologist Marin Medical Laboratories PathGroup SF ________________________________ Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BDeBrosse-Serra <@t> isisph.com Thu Oct 3 11:23:00 2013 From: BDeBrosse-Serra <@t> isisph.com (Bea DeBrosse-Serra) Date: Thu Oct 3 11:23:08 2013 Subject: [Histonet] RE: Microtomes In-Reply-To: References: Message-ID: Leica. Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bruce Gapinski Sent: Thursday, October 03, 2013 7:47 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Microtomes Dear Histonians I'm in the market (please, no vendors) for a new microtome. I'd like an automated/manual type. What is on the market these days that is worthy? I know about Leica, but it's been many years since I've looked for these instruments and would love to know what you like and don't like about your microtomes? Respectfully, Bruce Gapinsk HT (ASCP) Chief Histologist Marin Medical Laboratories PathGroup SF ________________________________ Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Haley.Huggins <@t> DignityHealth.org Thu Oct 3 12:22:07 2013 From: Haley.Huggins <@t> DignityHealth.org (Huggins, Haley - MRMC) Date: Thu Oct 3 12:22:13 2013 Subject: [Histonet] RE: Microtomes In-Reply-To: References: Message-ID: <4F36EC93A5737D4F8A2974E8FB8E260615F5730C6A@PHX-MSG-007-N2.chw.edu> I would go with Leica. I am getting them for my lab. Haley H. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bea DeBrosse-Serra Sent: Thursday, October 03, 2013 9:23 AM To: 'Bruce Gapinski'; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Microtomes Leica. Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bruce Gapinski Sent: Thursday, October 03, 2013 7:47 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Microtomes Dear Histonians I'm in the market (please, no vendors) for a new microtome. I'd like an automated/manual type. What is on the market these days that is worthy? I know about Leica, but it's been many years since I've looked for these instruments and would love to know what you like and don't like about your microtomes? Respectfully, Bruce Gapinsk HT (ASCP) Chief Histologist Marin Medical Laboratories PathGroup SF ________________________________ Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Thu Oct 3 13:03:35 2013 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Thu Oct 3 13:07:37 2013 Subject: [Histonet] HT HistoDeck question... In-Reply-To: References: <9F3CFEE76E51B64991C7485270890B40497D12D6@EX5.lj.gnf.org> <8FAB0141C65B42098CE4F110ECE9F372@HP2010> <351A66CE7FB11D40AB8AC8FE5559EC7E11A975A411@ushpwbmsmmp008.one.ads.bms.com> <335B9C2DCE414D1CB831DCCB07DEDC94@HP2010> Message-ID: We also use this oil red O method and use the 40% formaldehyde. The questions lacks enough information to correctly answer it. I am sure the author of the question had something in mind and other options didn't occur to him/her at the time. Jennifer From: "Grantham, Andrea L - (algranth)" To: Cc: HISTONET Date: 10/03/2013 08:33 AM Subject: Re: [Histonet] HT HistoDeck question... Sent by: histonet-bounces@lists.utsouthwestern.edu I'd go with "A", but it really depends on what you are going to do with the sections after fixation. In the protocol for Oil Red O in Freida's second edition (that I use almost daily combined with some steps from PolyScientific's ORO protocol), step #1 says to fix in 40% formaldehyde. Doesn't say vapors so I plunk the slides in the liquid 40%. I have fixed frozen sections for regular H&E and other stains as well in 40% formaldehyde and they come out beautiful. Unless there is some specific request or reason to use cold acetone or alcohol this is what I use. Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tony.auge <@t> gmail.com Thu Oct 3 13:20:17 2013 From: tony.auge <@t> gmail.com (Tony Auge) Date: Thu Oct 3 13:20:23 2013 Subject: [Histonet] RE: Microtomes In-Reply-To: <4F36EC93A5737D4F8A2974E8FB8E260615F5730C6A@PHX-MSG-007-N2.chw.edu> References: <4F36EC93A5737D4F8A2974E8FB8E260615F5730C6A@PHX-MSG-007-N2.chw.edu> Message-ID: Thermo Shandon Finesse is my favorite. Manual version requires oiling which is easy and gives you a good reason to keep it clean inside. I got one for about 6,000. The high end automatic version is the Finesse ME+ and is the best microtome I have ever seen. Someone that has never cut before could sit down and get perfect sections on their first block. The quote i got for that was about 12,000 which was too much for my lab. Tony Auge HTL (ASCP) QIHC Cell: (651) 373-4768 Email: tony.auge@gmail.com From cmiller <@t> physlab.com Thu Oct 3 13:31:09 2013 From: cmiller <@t> physlab.com (Cheri Miller) Date: Thu Oct 3 13:31:35 2013 Subject: [Histonet] Microtomes In-Reply-To: <1380815296.64587.YahooMailNeo@web163103.mail.bf1.yahoo.com> References: , <1380815296.64587.YahooMailNeo@web163103.mail.bf1.yahoo.com> Message-ID: Leica always Cheryl A. Miller HT ASCP cm Histology Supervisor Hygiene Officer Physicians Laboratory, P.C. 4140 F St. Omaha , NE. 68117 402 731 4145 ext. 532 Cell 402 493 0403 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa [rjbuesa@yahoo.com] Sent: Thursday, October 03, 2013 10:48 AM To: Bruce Gapinski; 'histonet@lists.utsouthwestern.edu' Subject: Re: [Histonet] Microtomes Still Leica Ren? J. ________________________________ From: Bruce Gapinski To: "'histonet@lists.utsouthwestern.edu'" Sent: Thursday, October 3, 2013 10:46 AM Subject: [Histonet] Microtomes Dear Histonians I'm in the market (please, no vendors) for a new microtome. I'd like an automated/manual type. What is on the market these days that is worthy? I know about Leica, but it's been many years since I've looked for these instruments and would love to know what you like and don't like about your microtomes? Respectfully, Bruce Gapinsk HT (ASCP) Chief Histologist Marin Medical Laboratories PathGroup SF ________________________________ Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From trathborne <@t> somerset-healthcare.com Thu Oct 3 13:33:56 2013 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Thu Oct 3 13:35:18 2013 Subject: [Histonet] HT HistoDeck question... In-Reply-To: References: <9F3CFEE76E51B64991C7485270890B40497D12D6@EX5.lj.gnf.org> <8FAB0141C65B42098CE4F110ECE9F372@HP2010> <351A66CE7FB11D40AB8AC8FE5559EC7E11A975A411@ushpwbmsmmp008.one.ads.bms.com> <335B9C2DCE414D1CB831DCCB07DEDC94@HP2010> Message-ID: <3AD061FE740D464FAC7BF6B5CFB75707A8A727C5@smcmail02.somerset-healthcare.com> For those of you who use the 40% formaldehyde, how long is you fixation time on frozen slides? We use 10% NBF with 1 minute to fix, but sometimes it gets hectic if you have multiple frozens all at once. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Thursday, October 03, 2013 2:04 PM To: Grantham, Andrea L - (algranth) Cc: HISTONET; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] HT HistoDeck question... We also use this oil red O method and use the 40% formaldehyde. The questions lacks enough information to correctly answer it. I am sure the author of the question had something in mind and other options didn't occur to him/her at the time. Jennifer From: "Grantham, Andrea L - (algranth)" To: Cc: HISTONET Date: 10/03/2013 08:33 AM Subject: Re: [Histonet] HT HistoDeck question... Sent by: histonet-bounces@lists.utsouthwestern.edu I'd go with "A", but it really depends on what you are going to do with the sections after fixation. In the protocol for Oil Red O in Freida's second edition (that I use almost daily combined with some steps from PolyScientific's ORO protocol), step #1 says to fix in 40% formaldehyde. Doesn't say vapors so I plunk the slides in the liquid 40%. I have fixed frozen sections for regular H&E and other stains as well in 40% formaldehyde and they come out beautiful. Unless there is some specific request or reason to use cold acetone or alcohol this is what I use. Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LSebree <@t> uwhealth.org Thu Oct 3 13:44:35 2013 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Thu Oct 3 13:44:40 2013 Subject: [Histonet] C3d by IHC on FFPE human specimens Message-ID: <77DD817201982748BC67D7960F2F76AF06C4A9@UWHC-MBX12.uwhis.hosp.wisc.edu> Does anyone in Histoland know of a reference lab that offers this? Thanks, Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 From benjamin <@t> histologistics.com Thu Oct 3 14:20:56 2013 From: benjamin <@t> histologistics.com (Benjamin) Date: Thu Oct 3 14:21:07 2013 Subject: [Histonet] HT HistoDeck question... In-Reply-To: <8FAB0141C65B42098CE4F110ECE9F372@HP2010> References: <9F3CFEE76E51B64991C7485270890B40497D12D6@EX5.lj.gnf.org> <8FAB0141C65B42098CE4F110ECE9F372@HP2010> Message-ID: <019671DE-7121-47E2-B51F-9474A3BA3F86@histologistics.com> It would dissolve the fat if you used acetone wouldnt it? Without knowing the tissue type or stain, the answer is A. All other choices dissolve fat I think. Good luck on your exam! Sent from my iPhone On Oct 3, 2013, at 8:15 AM, "Lee & Peggy Wenk" wrote: > Personally, I think it's "a" is a wrong answer, and that you are correct that "b" is a better answer. My students and I have found a couple of other questions that we thought had the wrong answer indicated in the study set. > > Peggy A. Wenk, HTL(ASCP)SLS > -----Original Message----- From: Stephenson, Sheryl > Sent: Thursday, October 03, 2013 7:21 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] HT HistoDeck question... > > Hi, > Please clarify why this answer to the HistoDeck study question is a) and not b). > > Here is the question: > > 'Frozen section slides cut from fresh, unfixed tissue specimens are optimally fixed in which of the following solutions? > a) 37%-40% formaldehyde > b) Cold acetone > c) Acetic acid alcohol > d) Alcoholic formalin > > Thanks, > > > Sheryl Stephenson | Histology Technician > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Thu Oct 3 14:21:45 2013 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Thu Oct 3 14:23:34 2013 Subject: AW: [Histonet] HT HistoDeck question... In-Reply-To: <3AD061FE740D464FAC7BF6B5CFB75707A8A727C5@smcmail02.somerset-healthcare.com> References: <9F3CFEE76E51B64991C7485270890B40497D12D6@EX5.lj.gnf.org> <8FAB0141C65B42098CE4F110ECE9F372@HP2010> <351A66CE7FB11D40AB8AC8FE5559EC7E11A975A411@ushpwbmsmmp008.one.ads.bms.com> <335B9C2DCE414D1CB831DCCB07DEDC94@HP2010> <3AD061FE740D464FAC7BF6B5CFB75707A8A727C5@smcmail02.somerset-healthcare.com> Message-ID: <000d01cec06d$ceb72380$6c256a80$@gmx.at> We use 36-40% formaldehyde for a minimal time of 2-3 dips for fast frozen HE. On the other side the slides stand in the solution as long as all frozens are already cut. Commercial formaldehyde contents a good part of methanol (a MSDS says 5-15%). So fast fixation is a combination of formaldehyde and alcohol fixation. Additional we use Harris hematoxylin, that's also mainly made of ethanol. So in my opinion all together fixation is rather due to alcohol in this way. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Rathborne, Toni Gesendet: Donnerstag, 03. Oktober 2013 20:34 An: 'Jennifer MacDonald'; Grantham, Andrea L - (algranth) Cc: HISTONET; histonet-bounces@lists.utsouthwestern.edu Betreff: RE: [Histonet] HT HistoDeck question... For those of you who use the 40% formaldehyde, how long is you fixation time on frozen slides? We use 10% NBF with 1 minute to fix, but sometimes it gets hectic if you have multiple frozens all at once. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Thursday, October 03, 2013 2:04 PM To: Grantham, Andrea L - (algranth) Cc: HISTONET; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] HT HistoDeck question... We also use this oil red O method and use the 40% formaldehyde. The questions lacks enough information to correctly answer it. I am sure the author of the question had something in mind and other options didn't occur to him/her at the time. Jennifer From: "Grantham, Andrea L - (algranth)" To: Cc: HISTONET Date: 10/03/2013 08:33 AM Subject: Re: [Histonet] HT HistoDeck question... Sent by: histonet-bounces@lists.utsouthwestern.edu I'd go with "A", but it really depends on what you are going to do with the sections after fixation. In the protocol for Oil Red O in Freida's second edition (that I use almost daily combined with some steps from PolyScientific's ORO protocol), step #1 says to fix in 40% formaldehyde. Doesn't say vapors so I plunk the slides in the liquid 40%. I have fixed frozen sections for regular H&E and other stains as well in 40% formaldehyde and they come out beautiful. Unless there is some specific request or reason to use cold acetone or alcohol this is what I use. Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jonesly <@t> mir.wustl.edu Thu Oct 3 14:32:51 2013 From: jonesly <@t> mir.wustl.edu (Jones, Lynne) Date: Thu Oct 3 14:33:02 2013 Subject: [Histonet] Question about fixation terminology Message-ID: Hello - Our research group does a fair amount of autoradiography with frozen sections and we sometimes perform IHC or routine stains. I am not a histologist (nor do we have one in our current group), so I assumed that the correct answer was alcoholic formalin, because the other options were either inappropriate or not fixatives. I was taught (by an old-school cell biologist) that both alcohol and acetone act as dehydrating agents when used on frozen sections, and are not true fixatives, because they don't cross-link proteins. "Real" fixatives don't just preserve against decay, they also modify the tissue (i.e., cross-linking or denaturing proteins). I am pretty sure I was taught that acetone does not "fix" tissue, and that "fixing" tissue to a slide is an imprecise/slang term derived from "affix". (This was at least a decade ago, so my memory could be faulty.) How is acetone a fixative? I thought it just replaced water, and preserved the structure of frozen sections by drying them out. (Please be kind - I'm not a histologist. I've sat at the cryostat sectioning mouse brains, and I know when we use it, but I don't understand what the acetone actually does.) How do you describe the difference between preserving tissue by drying (with acetone) and cross-linking the proteins (with alcoholic formalin)? I would really appreciate clarification from the guru's on HistoNet. I no longer spend much time in the lab, but I edit our manuscripts, so try make sure we use the correct terms in describing how the work is done. (Some reviewers get picky about terminology.) Thanks, Lynne Jones Lab Manager Radiochemistry Research Group Radiological Chemistry and Imaging Lab Washington University School of Medicine St. Louis, MO Message: 2 Date: Thu, 3 Oct 2013 10:21:42 -0400 From: "Lee & Peggy Wenk" > Subject: Re: [Histonet] HT HistoDeck question... To: "Watson, Linda" >, "Stephenson, Sheryl" >, > Message-ID: <335B9C2DCE414D1CB831DCCB07DEDC94@HP2010> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original I agree, there is probably more than one correct answer to this question, depending upon whether you are planning on doing stains for lipids, IHC, immunofluoresence or muscle enzymes. But I don't think (A) full strength 37-40% formaldehyde solution would ever be the correct answer. Unless you put a gauze in the bottom on the coplin jar, pour a little conc. formaldehyde on the gauze, put a lid on the coplin jar, and fix the section in formaldehyde vapors. But the question does say SOLUTION, not VAPOR. So I still think A is wrong. And most likely full strength acetic acid (C) is wrong - would eat the tissue off the slide. That leaves cold acetone (B) , which is good for some antibodies and some enzymes, or alcoholic formalin (D) which might be OK, but most of the time things either like alcohol and hate formalin, or they like formalin and don't like alcohol. So I would think most FS that we want to fix would not particularly like alcoholic formalin. And none of the solutions listed are good for lipids. So, given the question (with incomplete information) and the choices of answers, I would still side with (B) cold acetone. Now - a little aside - for the questions on the ASCP HT and HTL exams - if it is a new question, the people on the HT/HTL exam committee would be looking at it intensely before it goes on the exam for the first time. If the committee people are having problems answering it (like we are here), the question would be reworked until all the issues are resolved (such as putting in "for lymph node IHC" into the question). If it makes it past the committee, and the stats from the exam show that many people are having problems answering it, the question is pulled from the exam and is not used in the person's score. The question is then sent back to the HT/HTL exam committee, to try to figure out why examinees were having problems, and the question reworked again. As someone who has written exam questions at the school for 20+ years, I can tell you that it really is hard to write exam questions. I think I've covered everything in the question so that it is straight forward, and then half the students read something into the question that I never thought of, or come up with a written answer that I never considered. So I either have to throw out the question or give the point to the student, depending upon what's going on. And then mark the question for a re-write next year. And that doesn't include me marking the wrong answer on my master sheet. It happens! Peggy A. Wenk, HTL(ASCP)SLS -----Original Message----- From: Watson, Linda Sent: Thursday, October 03, 2013 9:40 AM To: Lee & Peggy Wenk ; Stephenson, Sheryl ; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HT HistoDeck question... For frozen cut sections, would the fixation also depend on what you plan on doing with it. For example, H&E, Special Stain or IHC? Please correct if I am wrong. I think that is a trick question!!! >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet- >bounces@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk >Sent: Thursday, October 03, 2013 8:16 AM >To: Stephenson, Sheryl; histonet@lists.utsouthwestern.edu >Subject: Re: [Histonet] HT HistoDeck question... > >Personally, I think it's "a" is a wrong answer, and that you are correct >that "b" is a better answer. My students and I have found a couple of >other >questions that we thought had the wrong answer indicated in the study >set. > >Peggy A. Wenk, HTL(ASCP)SLS >-----Original Message----- >From: Stephenson, Sheryl >Sent: Thursday, October 03, 2013 7:21 AM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] HT HistoDeck question... > >Hi, >Please clarify why this answer to the HistoDeck study question is a) >and >not b). > >Here is the question: > > 'Frozen section slides cut from fresh, unfixed tissue specimens are >optimally fixed in which of the following solutions? >a) 37%-40% formaldehyde >b) Cold acetone >c) Acetic acid alcohol >d) Alcoholic formalin > >Thanks, > > >Sheryl Stephenson | Histology Technician > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ________________________________ The material in this message is private and may contain Protected Healthcare Information (PHI). If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this email in error, please immediately notify the sender via telephone or return mail. From joelleweaver <@t> hotmail.com Thu Oct 3 15:21:11 2013 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Thu Oct 3 15:21:20 2013 Subject: [Histonet] Question about fixation terminology In-Reply-To: References: Message-ID: Surely I will find those that disagree with this post, however what I was "classically" trained about fixation categories generally falls within the information below...which I took the liberty of reposting here since it is pretty clear & straightforward from Leica. http://www.leicabiosystems.com/pathologyleaders/fixation-and-fixatives-1-the-process-of-fixation-and-the-nature-of-fixatives/ I hope this will help. " Traditionally fixing agents were termed ?coagulant? or ?non-coagulant? based on their effect on soluble proteins in solution. 7 Coagulant fixatives were said to result in a permeable meshwork of protein strands whereas non-coagulant fixatives which are additive in nature, formed extensive cross-links producing a less permeable gel. These terms are still encountered in modern histological literature but a more systematic approach has recently been taken to classification. 2 There are two major mechanisms which are important in fixation of proteins and protein complexes: denaturation, and addition and cross-link formation". Denaturation: " Most commonly this effect is induced by dehydrants such as the alcohols or acetone. These reagents remove and replace free water in cells and tissues and cause a change in the tertiary structure of proteins by destabilizing hydrophobic bonding. Hydrophobic areas, frequently found on the inside of protein molecules, are released from the repulsion of water and become free to occupy a greater area. hydrophilic areas of protein water molecules are loosely bound by hydrogen bonds and removal of water also destabilizes these bonds. The changes produced in the conformation of the protein molecules cause a change in the solubility of the protein, rendering water soluble proteins insoluble, a change that is largely irreversible if the protein is returned to an aqueous environment." 2, 7 Addition and cross-link formation: " The non-coagulant fixing agents chemically react with proteins and other cell and tissue components, becoming bound to them by addition and forming inter-molecular and intra-molecular cross-links. Because these agents are reactive compounds they bind to a variety of chemical groups in tissues, often affecting the charge at the site of attachment. This can have an effect on the subsequent staining characteristics of a particular protein as well as altering its molecular conformation and thus its solubility" . For further and more in depth discussion of formalin fixation ( and the article I am usually referred to in order to make corrections to my own chemistry in publications) is 1985!! Fox, et al. "Formaldehyde Fixation", Journal of Histochemistry and Cytochemistry, vol. 33, No. 8. pp. 845-855. pdf available at http://www.gofindpdf.com/readpdf/formaldehyde-fixation.html Joelle Weaver MAOM, HTL (ASCP) QIHC > From: jonesly@mir.wustl.edu > To: histonet@lists.utsouthwestern.edu > Date: Thu, 3 Oct 2013 19:32:51 +0000 > CC: > Subject: [Histonet] Question about fixation terminology > > Hello - > Our research group does a fair amount of autoradiography with frozen sections and we sometimes perform IHC or routine stains. I am not a histologist (nor do we have one in our current group), so I assumed that the correct answer was alcoholic formalin, because the other options were either inappropriate or not fixatives. > > I was taught (by an old-school cell biologist) that both alcohol and acetone act as dehydrating agents when used on frozen sections, and are not true fixatives, because they don't cross-link proteins. "Real" fixatives don't just preserve against decay, they also modify the tissue (i.e., cross-linking or denaturing proteins). I am pretty sure I was taught that acetone does not "fix" tissue, and that "fixing" tissue to a slide is an imprecise/slang term derived from "affix". (This was at least a decade ago, so my memory could be faulty.) > > How is acetone a fixative? I thought it just replaced water, and preserved the structure of frozen sections by drying them out. (Please be kind - I'm not a histologist. I've sat at the cryostat sectioning mouse brains, and I know when we use it, but I don't understand what the acetone actually does.) > > How do you describe the difference between preserving tissue by drying (with acetone) and cross-linking the proteins (with alcoholic formalin)? > > I would really appreciate clarification from the guru's on HistoNet. I no longer spend much time in the lab, but I edit our manuscripts, so try make sure we use the correct terms in describing how the work is done. (Some reviewers get picky about terminology.) > > Thanks, > > Lynne Jones > > Lab Manager > Radiochemistry Research Group > Radiological Chemistry and Imaging Lab > Washington University School of Medicine > St. Louis, MO > > > > > > > Message: 2 > > Date: Thu, 3 Oct 2013 10:21:42 -0400 > > From: "Lee & Peggy Wenk" > > > Subject: Re: [Histonet] HT HistoDeck question... > > To: "Watson, Linda" >, "Stephenson, Sheryl" > > >, > > > Message-ID: <335B9C2DCE414D1CB831DCCB07DEDC94@HP2010> > > Content-Type: text/plain; format=flowed; charset="iso-8859-1"; > > reply-type=original > > > > I agree, there is probably more than one correct answer to this question, > > depending upon whether you are planning on doing stains for lipids, IHC, > > immunofluoresence or muscle enzymes. > > > > But I don't think (A) full strength 37-40% formaldehyde solution would ever > > be the correct answer. Unless you put a gauze in the bottom on the coplin > > jar, pour a little conc. formaldehyde on the gauze, put a lid on the coplin > > jar, and fix the section in formaldehyde vapors. But the question does say > > SOLUTION, not VAPOR. So I still think A is wrong. And most likely full > > strength acetic acid (C) is wrong - would eat the tissue off the slide. > > > > That leaves cold acetone (B) , which is good for some antibodies and some > > enzymes, or alcoholic formalin (D) which might be OK, but most of the time > > things either like alcohol and hate formalin, or they like formalin and > > don't like alcohol. So I would think most FS that we want to fix would not > > particularly like alcoholic formalin. > > > > And none of the solutions listed are good for lipids. > > > > So, given the question (with incomplete information) and the choices of > > answers, I would still side with (B) cold acetone. > > > > Now - a little aside - for the questions on the ASCP HT and HTL exams - if > > it is a new question, the people on the HT/HTL exam committee would be > > looking at it intensely before it goes on the exam for the first time. If > > the committee people are having problems answering it (like we are here), > > the question would be reworked until all the issues are resolved (such as > > putting in "for lymph node IHC" into the question). If it makes it past the > > committee, and the stats from the exam show that many people are having > > problems answering it, the question is pulled from the exam and is not used > > in the person's score. The question is then sent back to the HT/HTL exam > > committee, to try to figure out why examinees were having problems, and the > > question reworked again. > > > > As someone who has written exam questions at the school for 20+ years, I can > > tell you that it really is hard to write exam questions. I think I've > > covered everything in the question so that it is straight forward, and then > > half the students read something into the question that I never thought of, > > or come up with a written answer that I never considered. So I either have > > to throw out the question or give the point to the student, depending upon > > what's going on. And then mark the question for a re-write next year. And > > that doesn't include me marking the wrong answer on my master sheet. It > > happens! > > > > Peggy A. Wenk, HTL(ASCP)SLS > > > > -----Original Message----- > > From: Watson, Linda > > Sent: Thursday, October 03, 2013 9:40 AM > > To: Lee & Peggy Wenk ; Stephenson, Sheryl ; > > histonet@lists.utsouthwestern.edu > > Subject: RE: [Histonet] HT HistoDeck question... > > > > For frozen cut sections, would the fixation also depend on what you plan on > > doing with it. For example, H&E, Special Stain or IHC? Please correct if I > > am wrong. I think that is a trick question!!! > > > > >-----Original Message----- > > >From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet- > > >bounces@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk > > >Sent: Thursday, October 03, 2013 8:16 AM > > >To: Stephenson, Sheryl; histonet@lists.utsouthwestern.edu > > >Subject: Re: [Histonet] HT HistoDeck question... > > > > > >Personally, I think it's "a" is a wrong answer, and that you are correct > > >that "b" is a better answer. My students and I have found a couple of > > >other > > >questions that we thought had the wrong answer indicated in the study > > >set. > > > > > >Peggy A. Wenk, HTL(ASCP)SLS > > >-----Original Message----- > > >From: Stephenson, Sheryl > > >Sent: Thursday, October 03, 2013 7:21 AM > > >To: histonet@lists.utsouthwestern.edu > > >Subject: [Histonet] HT HistoDeck question... > > > > > >Hi, > > >Please clarify why this answer to the HistoDeck study question is a) > > >and > > >not b). > > > > > >Here is the question: > > > > > > 'Frozen section slides cut from fresh, unfixed tissue specimens are > > >optimally fixed in which of the following solutions? > > >a) 37%-40% formaldehyde > > >b) Cold acetone > > >c) Acetic acid alcohol > > >d) Alcoholic formalin > > > > > >Thanks, > > > > > > > > >Sheryl Stephenson | Histology Technician > > > > > > > > > > > > > > >_______________________________________________ > > >Histonet mailing list > > >Histonet@lists.utsouthwestern.edu > > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ________________________________ > > The material in this message is private and may contain Protected Healthcare Information (PHI). If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this email in error, please immediately notify the sender via telephone or return mail. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sdysart <@t> mirnarx.com Thu Oct 3 15:28:44 2013 From: sdysart <@t> mirnarx.com (Sarah Dysart) Date: Thu Oct 3 15:28:52 2013 Subject: [Histonet] RE: Question about fixation terminology In-Reply-To: References: Message-ID: http://www.ihcworld.com/_protocols/general_ICC/fixation.htm This article specifically addresses the differences. You are right about the cross linking, but alcohol and acetone are still considered fixatives. Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jones, Lynne Sent: Thursday, October 03, 2013 2:33 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Question about fixation terminology Hello - Our research group does a fair amount of autoradiography with frozen sections and we sometimes perform IHC or routine stains. I am not a histologist (nor do we have one in our current group), so I assumed that the correct answer was alcoholic formalin, because the other options were either inappropriate or not fixatives. I was taught (by an old-school cell biologist) that both alcohol and acetone act as dehydrating agents when used on frozen sections, and are not true fixatives, because they don't cross-link proteins. "Real" fixatives don't just preserve against decay, they also modify the tissue (i.e., cross-linking or denaturing proteins). I am pretty sure I was taught that acetone does not "fix" tissue, and that "fixing" tissue to a slide is an imprecise/slang term derived from "affix". (This was at least a decade ago, so my memory could be faulty.) How is acetone a fixative? I thought it just replaced water, and preserved the structure of frozen sections by drying them out. (Please be kind - I'm not a histologist. I've sat at the cryostat sectioning mouse brains, and I know when we use it, but I don't understand what the acetone actually does.) How do you describe the difference between preserving tissue by drying (with acetone) and cross-linking the proteins (with alcoholic formalin)? I would really appreciate clarification from the guru's on HistoNet. I no longer spend much time in the lab, but I edit our manuscripts, so try make sure we use the correct terms in describing how the work is done. (Some reviewers get picky about terminology.) Thanks, Lynne Jones Lab Manager Radiochemistry Research Group Radiological Chemistry and Imaging Lab Washington University School of Medicine St. Louis, MO Message: 2 Date: Thu, 3 Oct 2013 10:21:42 -0400 From: "Lee & Peggy Wenk" > Subject: Re: [Histonet] HT HistoDeck question... To: "Watson, Linda" >, "Stephenson, Sheryl" >, > Message-ID: <335B9C2DCE414D1CB831DCCB07DEDC94@HP2010> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original I agree, there is probably more than one correct answer to this question, depending upon whether you are planning on doing stains for lipids, IHC, immunofluoresence or muscle enzymes. But I don't think (A) full strength 37-40% formaldehyde solution would ever be the correct answer. Unless you put a gauze in the bottom on the coplin jar, pour a little conc. formaldehyde on the gauze, put a lid on the coplin jar, and fix the section in formaldehyde vapors. But the question does say SOLUTION, not VAPOR. So I still think A is wrong. And most likely full strength acetic acid (C) is wrong - would eat the tissue off the slide. That leaves cold acetone (B) , which is good for some antibodies and some enzymes, or alcoholic formalin (D) which might be OK, but most of the time things either like alcohol and hate formalin, or they like formalin and don't like alcohol. So I would think most FS that we want to fix would not particularly like alcoholic formalin. And none of the solutions listed are good for lipids. So, given the question (with incomplete information) and the choices of answers, I would still side with (B) cold acetone. Now - a little aside - for the questions on the ASCP HT and HTL exams - if it is a new question, the people on the HT/HTL exam committee would be looking at it intensely before it goes on the exam for the first time. If the committee people are having problems answering it (like we are here), the question would be reworked until all the issues are resolved (such as putting in "for lymph node IHC" into the question). If it makes it past the committee, and the stats from the exam show that many people are having problems answering it, the question is pulled from the exam and is not used in the person's score. The question is then sent back to the HT/HTL exam committee, to try to figure out why examinees were having problems, and the question reworked again. As someone who has written exam questions at the school for 20+ years, I can tell you that it really is hard to write exam questions. I think I've covered everything in the question so that it is straight forward, and then half the students read something into the question that I never thought of, or come up with a written answer that I never considered. So I either have to throw out the question or give the point to the student, depending upon what's going on. And then mark the question for a re-write next year. And that doesn't include me marking the wrong answer on my master sheet. It happens! Peggy A. Wenk, HTL(ASCP)SLS -----Original Message----- From: Watson, Linda Sent: Thursday, October 03, 2013 9:40 AM To: Lee & Peggy Wenk ; Stephenson, Sheryl ; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HT HistoDeck question... For frozen cut sections, would the fixation also depend on what you plan on doing with it. For example, H&E, Special Stain or IHC? Please correct if I am wrong. I think that is a trick question!!! >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet- >bounces@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk >Sent: Thursday, October 03, 2013 8:16 AM >To: Stephenson, Sheryl; histonet@lists.utsouthwestern.edu >Subject: Re: [Histonet] HT HistoDeck question... > >Personally, I think it's "a" is a wrong answer, and that you are correct >that "b" is a better answer. My students and I have found a couple of >other >questions that we thought had the wrong answer indicated in the study >set. > >Peggy A. Wenk, HTL(ASCP)SLS >-----Original Message----- >From: Stephenson, Sheryl >Sent: Thursday, October 03, 2013 7:21 AM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] HT HistoDeck question... > >Hi, >Please clarify why this answer to the HistoDeck study question is a) >and >not b). > >Here is the question: > > 'Frozen section slides cut from fresh, unfixed tissue specimens are >optimally fixed in which of the following solutions? >a) 37%-40% formaldehyde >b) Cold acetone >c) Acetic acid alcohol >d) Alcoholic formalin > >Thanks, > > >Sheryl Stephenson | Histology Technician > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ________________________________ The material in this message is private and may contain Protected Healthcare Information (PHI). If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this email in error, please immediately notify the sender via telephone or return mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From deganh <@t> upstate.edu Thu Oct 3 15:38:15 2013 From: deganh <@t> upstate.edu (Helene Degan) Date: Thu Oct 3 15:38:21 2013 Subject: [Histonet] Bodian Message-ID: <524D9D770200007A0002721B@gatedom1.upstate.edu> Hi Thanks to every one that sent me Acid Phosphotase procedure for muscle biopsies. I also need some help with the bodian stain, we are not getting any staining on the slides, we have a procedure that they used years ago and now we can't get it working (we did purchase new chemicals) and we also tried a stain kit with very little results. Thanks again Helene Upstate Medical University From csegovia <@t> nsalabs.com Thu Oct 3 16:40:17 2013 From: csegovia <@t> nsalabs.com (Segovia, Claudia) Date: Thu Oct 3 16:40:24 2013 Subject: [Histonet] Proteinase K treatment Message-ID: <4EEF161B-5C24-481C-B35C-3A917FDDE2AA@nsalabs.com> Hello histoneters! This is my first time using the net. I hope to find here some answers to my problem. I am working with free floating sections embedded in gelatin. I need to use Proteinase K to detect alpha- synuclein aggregates in brain tissue. I tried concentrations in the range of 1 Ug/ml to 5Ug/ml, incubation time is 10 minutes at room temperature. I noticed that after several rinses the Proteinase K keeps being active, because the gelatin in the sections gets sticky and even disappears overtime. I did several rinses (4 X 10 min each) and I am afraid that the antibody will get degraded in the overnight incubation. How can I stop this enzymatic reaction? I will truly appreciate any insights, Thank you! Claudia N. Segovia Senior Neurohistologist Antibody Specialist NeuroScience Associates 10915 Lake Ridge Drive Knoxville, TN 37934 865-675-2245 csegovia@nsalabs.com STATEMENT OF CONFIDENTIALITY: The information contained in this electronic message and any attachments are intended for the exclusive use of the addressee(s) and may contain confidential or privileged information. If you are not the intended recipient, or the person responsible for delivering email to the intended recipient, be advised you have received this message in error and any use, dissemination, forwarding, printing or copying is strictly prohibited. Please notify NeuroScience Associates immediately at 865-675-2245 or at csegovia@nsalabs.com, and destroy all copies of this message and any attachments. You will be reimbursed for reasonable costs incurred in notifying us. From JMacDonald <@t> mtsac.edu Thu Oct 3 16:53:59 2013 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Thu Oct 3 16:54:10 2013 Subject: [Histonet] HT HistoDeck question... In-Reply-To: <3AD061FE740D464FAC7BF6B5CFB75707A8A727C5@smcmail02.somerset-healthcare.com> References: <9F3CFEE76E51B64991C7485270890B40497D12D6@EX5.lj.gnf.org> <8FAB0141C65B42098CE4F110ECE9F372@HP2010> <351A66CE7FB11D40AB8AC8FE5559EC7E11A975A411@ushpwbmsmmp008.one.ads.bms.com> <335B9C2DCE414D1CB831DCCB07DEDC94@HP2010> <3AD061FE740D464FAC7BF6B5CFB75707A8A727C5@smcmail02.somerset-healthcare.com> Message-ID: the protocol calls for 1 minute in the 40% formaldehyde and then rinse the sections well. From: "Rathborne, Toni" To: "'Jennifer MacDonald'" , "Grantham, Andrea L - (algranth)" Cc: HISTONET , "histonet-bounces@lists.utsouthwestern.edu" Date: 10/03/2013 11:42 AM Subject: RE: [Histonet] HT HistoDeck question... Sent by: histonet-bounces@lists.utsouthwestern.edu For those of you who use the 40% formaldehyde, how long is you fixation time on frozen slides? We use 10% NBF with 1 minute to fix, but sometimes it gets hectic if you have multiple frozens all at once. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [ mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Thursday, October 03, 2013 2:04 PM To: Grantham, Andrea L - (algranth) Cc: HISTONET; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] HT HistoDeck question... We also use this oil red O method and use the 40% formaldehyde. The questions lacks enough information to correctly answer it. I am sure the author of the question had something in mind and other options didn't occur to him/her at the time. Jennifer From: "Grantham, Andrea L - (algranth)" To: Cc: HISTONET Date: 10/03/2013 08:33 AM Subject: Re: [Histonet] HT HistoDeck question... Sent by: histonet-bounces@lists.utsouthwestern.edu I'd go with "A", but it really depends on what you are going to do with the sections after fixation. In the protocol for Oil Red O in Freida's second edition (that I use almost daily combined with some steps from PolyScientific's ORO protocol), step #1 says to fix in 40% formaldehyde. Doesn't say vapors so I plunk the slides in the liquid 40%. I have fixed frozen sections for regular H&E and other stains as well in 40% formaldehyde and they come out beautiful. Unless there is some specific request or reason to use cold acetone or alcohol this is what I use. Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From chardy <@t> csu.edu.au Thu Oct 3 17:43:22 2013 From: chardy <@t> csu.edu.au (Hardy, Cate) Date: Thu Oct 3 17:43:36 2013 Subject: [Histonet] RE:Gram stain (Mesru T) Histonet Digest, Vol 119, Issue 4 In-Reply-To: <20131003140646.9A5722CB7D_24D79F6F@seaprod02.csumain.csu.edu.au> References: <20131003140646.9A5722CB7D_24D79F6F@seaprod02.csumain.csu.edu.au> Message-ID: <144C8E2433CA3B4D9E080E5CDA803D0597623BC68C@MAIL01.CSUMain.csu.edu.au> We perform Gram Twort for our gram stains. It is essentially the same as the gram stain used in microbiology except that we counter stain with a neutral red fast green which is very effective. Cheers Cate Cate Hardy Senior Technical Officer Veterinary Diagnostic Laboratory School of Animal and Veterinary Sciences Locked bag 588 Charles Sturt University Wagga Wagga NSW 2678 T: 02 69334000 Email: chardy@csu.edu.au Email: vdl@csu.edu.au -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Friday, 4 October 2013 12:07 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 119, Issue 4 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Gram stain (Mesru T) 2. Benchmark Ultra troubles (Gudrun Lang) 3. Re: RE: MOHS IPs (Kim Donadio) 4. Re: IHC turnaround time (Kim Donadio) 5. Re: Gram stain (Jennifer MacDonald) 6. Hi (Helene Degan) 7. RE: Validating new Benchmark instrument (Eytalis, Robert A) 8. need lever clamp for blade of leica microtome (Patricia F Lott) 9. Re: Shipping to Countries Outside of the US (Jan Shivers) 10. RE: Histotechnologist, (ASCP, QIHC certified) and Pathology assistant position in Seattle area (Yan Liang) 11. Vacuum and pressure in tissue processing (Teri Johnson) 12. Trivew (Gerald Davydov) 13. Inconsistent Sections with Cryostat (Perow, Elliott S (PEROWES10)) 14. HT HistoDeck question... (Stephenson, Sheryl) 15. RE: Inconsistent Sections with Cryostat (Manfre, Philip) 16. Re: HT HistoDeck question... (Lee & Peggy Wenk) 17. Re: RE: Inconsistent Sections with Cryostat (Lee & Peggy Wenk) 18. RE: HT HistoDeck question... (Watson, Linda) 19. RE: HT HistoDeck question... (Bernice Frederick) 20. The NSH was AMAZING!!! Congratulations to the 2013 Leadership, Education and Advocacy Award Winners!! From Pam Barker and RELIA Solutions!! (Pam Barker) ---------------------------------------------------------------------- Message: 1 Date: Wed, 2 Oct 2013 13:11:22 -0400 From: Mesru T Subject: [Histonet] Gram stain To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hi All, Does anybody have a protocol for Gram stain on FFPE mouse intestine sections. We are looking to distinguish between Klebsiella and Vancomycin Resistant Enterococcus (VRE). I would like to avoid using Picric acid as some protocols suggest. Thanks in advance. ------------------------------ Message: 2 Date: Wed, 2 Oct 2013 19:38:56 +0200 From: "Gudrun Lang" Subject: [Histonet] Benchmark Ultra troubles To: Message-ID: <001a01cebf96$45a20a10$d0e61e30$@gmx.at> Content-Type: text/plain; charset="us-ascii" Hi Ventana-users, we have a special technical problem with our Ultra. It seems, that the liquid in the waste-tube is sometime pressed out of the hole on the platform like a fountain. You can imagine the nice surprise, when the oil drops from the Ultra-roof onto the carousel-top. Everything is oily. The technical service has'nt found a cause until now. The filter in the tube has been changed. Has anyone seen a similar problem? Solved the problem? Gudrun Lang ------------------------------ Message: 3 Date: Wed, 2 Oct 2013 13:47:22 -0400 From: Kim Donadio Subject: Re: [Histonet] RE: MOHS IPs To: "Bauer, Karen L." Cc: "histonet@lists.utsouthwestern.edu" Message-ID: <628C0E60-8664-4DBC-B558-F3B5D9FEABF8@yahoo.com> Content-Type: text/plain; charset=us-ascii What detection kit are you using? Sent from my iPhone On Oct 2, 2013, at 10:47 AM, "Bauer, Karen L." wrote: > Thanks for the reply, > > We are pre-fixing in a formalin substitute, but we'll give the acetone a try. I have diluted the protease, but have not gotten any good results. We'll keep trying with that as well. > > We've tried multiple incubation times for the cytokeratin... From 5 minutes all the way up to 30 minutes... With no luck. > > We are using an enhanced polymer DAB. > > Thanks for the suggestions! I greatly appreciate it! :) > > Karen > > Karen L. Bauer HTL/HT (ASCP) | Histology Supervisor | Pathology | MOHS > Lab Supervisor | Dermatology | Phone: 715-838-3205 | > bauer.karen@mayo.edu | Mayo Clinic Health System | 1221 Whipple Street > | Eau Claire, WI 54702 | mayoclinichealthsystem.org > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > pruegg@ihctech.net > Sent: Wednesday, October 02, 2013 9:36 AM > To: Barbara Tibbs; Bauer, Karen L.; 'histonet@lists.utsouthwestern.edu' > Subject: RE: [Histonet] RE: MOHS IPs > > > I agree with Barbara try fixing in cold acetone/ethanol mix even = > just for a couple of minutes then go directly to buffer do not dry > after=x, I would dilute the protease 1:4 with buffer (PBS or TBS > what ever u u=) and do it for a short time. Enzyme digestion on > frozen sections i=ricky but fixing a little and diluting the > enzyme should help prevent ov= digestion. How long are u > incubating the primary? Are u usin=n enhanced labeled polymer > detection? HRP/Dab or AEC or Alk.P/fast=d? > > > > > > > > > -------- Original Message -------- > > Subject: [Histonet] RE: MOHS =s > > From: Barbara Tibbs <[1]barbara.tibbs@accuratediagnosticlabs.com> > > D=e: Wed, October 02, 2013 5:54 am > > To: "Bauer, Karen L." <[2]Bauer.Karen@mayo.edu>, > > "'histonet@lists.utsouthwest=n.edu'" > > <[3]histonet@lists.utsouthwestern.edu> > > > > Hello Karen, > =A > > Back in the old days of doing IHC manually on mostly fresh, froze n tissue I would immediately place the slide with the frozen section > into a=plin jar of acetone to fix it. Keep the coplin jar of > acetone in the cr=stat. Leave it in the acetone for only 10 > minutes. Try diluting the Pr=ease 2 - 1:1 to make it gentler so it > doesn't "eat" the tissue. This te=nique worked well when I was > doing ER/PR on frozen breast tumors. Not su= it will work with skin > but it's worth a try. > > > > Barbara S. Tib= > > Histology Supervisor > > Accurate Diagnostic Labs > > South Pl=nfield, NJ > > [4]barbara.tibbs@accuratediagnosticlabs.com > > 732-839-3374 > > C=l: 610-809-6508 > > > > > > _____________________________________=_ > > From: [5]histonet-bounces@lists.utsouthwestern.edu > <[6]histonet-bounces@lists.utsouthwestern=du> on behalf of Bauer, > Karen L. <[7]Bauer.Karen@mayo.edu> > > Sent: Tuesday, October 01, 20 5:22 PM > > To: '[8]=stonet@lists.utsouthwestern.edu' > > Subject: [Histonet] MOHS IPs > > > Hi all, > > > > We are in the process of validating some a=ibodies in our MOHS > lab. The Melan A (Mart 1) antibody is working well, =t it could be > darker. We will be increasing the Ab incubation time to se=f that > will help. > > > > As for the Pan Keratin, we cannot get it = work at all. We use > Protease 2 on our Ultra stainer for FFPE tissues in=stology, but > this stain in MOHS is placed on fresh, unfixed tissue... by=anual > drop method. Any time we've tried to use an enzyme retrieval, th= > tissue looks eaten up... even if we incubate if for a minute. > > > =ASince the tissue is not fixed, I figured that no retrieval step > would be=eded, but the Pan Keratin refuses to work with or without > retrieval. > =A > > For those of you in MOHS labs that are using a manual staining me thod for Melan A and Pan Keratin, would you be willing to share your > protoc= with us? > > > > Thanks so much!! > > > > Karen > > > > K=en L. Bauer HTL/HT (ASCP) | Histology Supervisor | Pathology | > MOHS Lab S=ervisor | Dermatology | Phone: 715-838-3205 | > [9]bauer.karen@mayo.edu<[10]mailto:bauer.karen@mayo.edu> | Mayo Clinic > Health System | 122=hipple Street | Eau Claire, WI 54702 | > [11]mayoclinichealthsystem.org rg/> > > > =A > > _______________________________________________ > > Histonet ma=ing list > > [13]Histo=t@lists.utsouthwestern.edu > > [14]http://lists.utsouthwestern.edu/mailman/l=tinfo/histonet > > > > _________________________________________=____ > > Histonet mailing list > > [15]Histonet@lists.utsouthwestern.edu > > [16]http://lists.utso=hwestern.edu/mailman/listinfo/histonet > > > > > > > > References > > 1. 3D"mailto:barbara.tibbs@accurated 2. ="mailto:Bauer.Karen@mayo.edu" > 3. 3D"mailto:histonet@lists.utsouthwestern.edu 4. 3D"mailto:barbara.tibbs@accuratediagnosticlabs.c 5. 3D"mailto:histonet-bounces@lists.utsouthwestern.edu 6. 3D"mailto:histo 7. 3D"mailto:Bauer.Karen 8. 3D"mailto:histonet@lists.utsouthwestern.edu" > 9. 3D"mailto:bauer.kar 10. 3D"mailto:bauer.karen@mayo 11. > 3D"http://mayoclinichealt= 12. 3D"http:/ 13. 3D"mailto:Histonet@lists.utsouthwestern.edu" > 14. 3D"http://lists.utsouthweste= > 15. 3D"mailto:Histonet@lists.u 16. > file://localhost/tmp/3D"h_____________________________________________ > __ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Wed, 2 Oct 2013 13:51:59 -0400 From: Kim Donadio Subject: Re: [Histonet] IHC turnaround time To: "McKenzie, Emily" Cc: "histonet@lists.utsouthwestern.edu" Message-ID: <75CAA15B-C45D-443E-87E9-E76143144E69@yahoo.com> Content-Type: text/plain; charset=utf-8 In less than 8 hours I can get 96 Ihc stains done with our dako link and that is two runs Sent from my iPhone On Oct 1, 2013, at 1:15 PM, "McKenzie, Emily" wrote: > Hello all, > I am currently working on a Six Sigma Green Belt project and am looking for some data. Can any one give me their general turnaround times for IHC, from time of order to time of delivery? > Thanks for all your help with this, > > Emily K. McKenzie BS, HT(ASCP) > > Memorial Medical Center???701 North First Street???Springfield, IL > 62781 > Ph: 217-788-3991???email: McKenzie.Emily@mhsil.com > > > > > ________________________________ > This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Wed, 2 Oct 2013 11:15:17 -0700 From: Jennifer MacDonald Subject: Re: [Histonet] Gram stain To: Mesru T Cc: histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" We have had great success with the Twort gram stain. From: Mesru T To: histonet@lists.utsouthwestern.edu Date: 10/02/2013 10:13 AM Subject: [Histonet] Gram stain Sent by: histonet-bounces@lists.utsouthwestern.edu Hi All, Does anybody have a protocol for Gram stain on FFPE mouse intestine sections. We are looking to distinguish between Klebsiella and Vancomycin Resistant Enterococcus (VRE). I would like to avoid using Picric acid as some protocols suggest. Thanks in advance. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Wed, 02 Oct 2013 14:39:26 -0400 From: "Helene Degan" Subject: [Histonet] Hi To: Message-ID: <524C301E0200007A000271AE@gatedom1.upstate.edu> Content-Type: text/plain; charset=US-ASCII Hi Im looking for an acid phosphatase enzyme procedure that might be out there the one we tried in the past was difficult to work up and get good reproducible results. Thanks Helene D Upstate University Medical Syracuse, NY ------------------------------ Message: 7 Date: Wed, 2 Oct 2013 18:40:31 +0000 From: "Eytalis, Robert A" Subject: [Histonet] RE: Validating new Benchmark instrument To: Fawn Bomar , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" I am thinking there has to be a way to integrate the Validation into the Optimization the vendor does. I think you should be able to make microarray blocks/slides of at least 10 positive and more for ERPR per CAP during the optimization. Now, the vendor will say they only do Optimization, but there this is a way to get them to do the stains for you at least. You can create your own document for the Validation. When your manager negotiates the cost of the analyzer, see if the vendor can help out with the initial expense with an allowance. It sounds like you might be past this point. Has anyone else done it this way? Robert A. Eytalis Laboratory Manager robert-eytalis@riversidehealthcare.net Phone: (815) 935-7256 ext. 5186 (815) 935-7535 Fax (815) 935-7068 Riverside Medical Center 350 N. Wall Street - Kankakee, IL 60901 http://mail.riversidehealthcare.net/owa/redir.aspx?C=qIzWpCGMNkq2SuiIt1v_2X7GmC1aOtAI7VlOiEQ974hhCQzwyDH6yJklmjoBIK92OWDFwivhIZs.&URL=http%3a%2f%2fwww.riversidemc.net%2f | http://mail.riversidehealthcare.net/owa/redir.aspx?C=qIzWpCGMNkq2SuiIt1v_2X7GmC1aOtAI7VlOiEQ974hhCQzwyDH6yJklmjoBIK92OWDFwivhIZs.&URL=http%3a%2f%2fwww.facebook.com%2fRiversideMC ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Fawn Bomar [Fawn.Bomar@HalifaxRegional.com] Sent: Tuesday, October 01, 2013 12:06 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Validating new Benchmark instrument Hi everyone, I know that this subject has come up several times and I am sorry to bother everyone again but I was hoping to find some help. We are upgrading our Benchmark Immuno machines to the Benchmark XT immuno machine at the end of October. Does anyone have any suggestions on how to approach the validation process? Do we need to validate every antibody that we use as well as the kits? I know that there is also a gray area surrounding how many slides need to be run to consider the antibody validated, but what would be the suggested amount of slides to validate a new machine as I know this will be very costly. Does anybody have a log/checklist/spreadsheet that they would be willing to share to help document this validation of the machine and each of the antibodies? Thank you in advance for your help Fawn Bomar ------------------------------------------------------------- This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Wed, 2 Oct 2013 18:51:22 +0000 From: Patricia F Lott Subject: [Histonet] need lever clamp for blade of leica microtome To: "histonet@lists.utsouthwestern.edu" Message-ID: <372C4AF089B6AE48B36F064FA891FF781380924D@UABEXMB3.ad.uab.edu> Content-Type: text/plain; charset="us-ascii" Does anyone have an extra lever clamp for a Leica microtome? I'd be happy to pay for it, and of course, for shipping! Thanks, Patty Lott UAB CMBD Core Lab 205-934-2007 ------------------------------ Message: 9 Date: Wed, 2 Oct 2013 14:03:38 -0500 From: Jan Shivers Subject: Re: [Histonet] Shipping to Countries Outside of the US To: Sheila Miller Cc: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=ISO-8859-1 According to our Receiving department, each country/institution has its own permit requirements for receiving samples from outside of their own country. It is up to the shipper to inquire of the receiver as to what permits are needed to ship to that country (Germany, in your case). My source also said that FedEx would also have good information on this, since they ship nearly everywhere around the globe. Jan Shivers On Wed, Oct 2, 2013 at 11:10 AM, Sheila Miller wrote: > Hello All, > > A question was posed to me by our Cancer Resource Department... > > I am wondering if you/lab has had experience with shipping tissue > specimens outside the country. We have a study where we may be doing this > and they are asking if we have to have a special permit to ship outside our > country. I believe it is going to Germany. > > Thank you, > Sheila > > > ____________________________________ > > This message and attachment(s), if any, is intended for the sole use of > the individual and/or entity > of which it is addressed, and may contain information that is > privileged,confidential and prohibited > from disclosure under applicable law. If you are not the addressee, or > authorized to receive this on > behalf of the addressee, you are hereby notified that you may not use, > copy, disclose or distribute > to anyone this message or any part thereof. If you have received this in > error, please immediately advise > the sender by e-mail and delete this information and all attachments from > your computer and network. > Thank you. > ____________________________________ > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 10 Date: Wed, 2 Oct 2013 14:24:21 -0500 From: Yan Liang Subject: [Histonet] RE: Histotechnologist, (ASCP, QIHC certified) and Pathology assistant position in Seattle area To: Brannon Owens , "histonet@lists.utsouthwestern.edu" Message-ID: <8DC126BD0D840947A161BBF46AB31688035AE3305C@DFW1MBX22.mex07a.mlsrvr.com> Content-Type: text/plain; charset="iso-8859-1" Needing a Histotechnologist (ASCP, QIHC certified) or Scientist (Histopathology) for a Seattle area. Five years of experience in routine histology including special stains, IHC and pathology knowledge. Send an updated resume to yliang@numirabio.com to be considered. A full job description is on the Numirabio.com .............................................. Yan Liang ?Ph.D. Senior Director, Histopathology Numira Biosciences Inc. 2023 120th Ave NE Bellevue, WA 98005 Phone: 425-777-4950 ext 201 Cell: 425-830-3090 Fax: 425-777-4949 yliang@numirabio.com www.numirabio.com www.altaportal.numirabio.com ------------------------------ Message: 11 Date: Wed, 2 Oct 2013 22:57:22 +0000 From: Teri Johnson Subject: [Histonet] Vacuum and pressure in tissue processing To: "histonet@lists.utsouthwestern.edu" Message-ID: <9F3CFEE76E51B64991C7485270890B40497D12D6@EX5.lj.gnf.org> Content-Type: text/plain; charset="us-ascii" Dear friends, I recall hearing at a conference (or maybe it was just a casual conversation by an expert during a NSH symposium break) that vacuum and pressure in tissue processing really accomplishes very little. I do believe that using heat and agitation of the solutions provides more activity kinetically and therefore makes processing more efficient. Can someone affirm or deny the efficacy of vacuum and/or pressure in tissue processing, please? Thank you, as always, for your wisdom. Teri Johnson Manager, Histology Genomics Institute for Novartis Research Foundation San Diego, CA 858-332-4752 ------------------------------ Message: 12 Date: Wed, 2 Oct 2013 21:04:01 -0400 From: Gerald Davydov Subject: [Histonet] Trivew To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Please share protocol for Triview from Zetta Corporation for Venatna. ------------------------------ Message: 13 Date: Thu, 3 Oct 2013 04:32:48 +0000 From: "Perow, Elliott S (PEROWES10)" Subject: [Histonet] Inconsistent Sections with Cryostat To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hello All, I have a Leica Cryocut 1800 with a Reichert-Jung 2020 microtome that has sat for 8 years and went through a move from one academic building to another. I am trying to section brook trout brain, but am having difficulty getting consistent sections. I will get a good section and then the next section will be a very very thin shaving of usually just the top portion of the OCT block. I have tried adjusting the clearance angle, made sure the blade and specimen is secured, replaced the blade with a new one, have tried different sectioning speeds, different thicknesses of the section and still continually only get a decent section every other turn. I was wondering if anyone has had any similar experiences or if maybe the machine just needs a maintenance check due to it being an older machine. I wasn't sure if the advancement mechanism may be off or if it is another issue that doesn't seem apparent to me. Any help would be greatly appreciated. Thanks, Elliott Perow Juniata College Biology POE CONFIDENTIALITY NOTICE: The materials in this electronic mail transmission (including all attachments) are private and confidential and are the property of the sender. The information contained in the material is privileged and is intended only for the use of the named addressee(s). If you are not the intended addressee, be advised that any unauthorized disclosure, copying, distribution or the taking of any action in reliance on the contents of this material is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender by replying to the e-mail, and then destroy it immediately. Thank you. ------------------------------ Message: 14 Date: Thu, 3 Oct 2013 06:21:14 -0500 From: "Stephenson, Sheryl" Subject: [Histonet] HT HistoDeck question... To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Hi, Please clarify why this answer to the HistoDeck study question is a) and not b). Here is the question: 'Frozen section slides cut from fresh, unfixed tissue specimens are optimally fixed in which of the following solutions? a) 37%-40% formaldehyde b) Cold acetone c) Acetic acid alcohol d) Alcoholic formalin Thanks, Sheryl Stephenson | Histology Technician _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Thu, 3 Oct 2013 07:54:56 -0400 From: "Manfre, Philip" Subject: [Histonet] RE: Inconsistent Sections with Cryostat To: "Perow, Elliott S (PEROWES10)" , "histonet@lists.utsouthwestern.edu" Message-ID: <558A4571351D0C42BD923F403F4198C4B27103726B@USCTMXP51014.merck.com> Content-Type: text/plain; charset="us-ascii" I believe you may need to have the unit serviced. It sounds like something is not tight enough, perhaps the stage or blade holder unit. You said you secured everything which makes me think you have some issue with the cryostat itself. If a sharp blade, tightened blade and specimen, and varying the speed doesn't work, then you may need professional assistance form a service technician, especially if it is an older unit that has been moved around. Have you tried different temperatures? I don't think that is the problem but it is worth a try. Good luck! Cryosectioning can be an art in itself. Those decent sections are likely thicker than you intend, by the way. Classic "thick and thin". Philip Manfre, B.A., HT (ASCP) Associate Principal Scientist Merck Research Laboratories WP45-251 PO Box 4 West Point, PA 19486 215-652-9750 215-993-0383 (fax) philip_manfre@merck.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Perow, Elliott S (PEROWES10) Sent: Thursday, October 03, 2013 12:33 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Inconsistent Sections with Cryostat Hello All, I have a Leica Cryocut 1800 with a Reichert-Jung 2020 microtome that has sat for 8 years and went through a move from one academic building to another. I am trying to section brook trout brain, but am having difficulty getting consistent sections. I will get a good section and then the next section will be a very very thin shaving of usually just the top portion of the OCT block. I have tried adjusting the clearance angle, made sure the blade and specimen is secured, replaced the blade with a new one, have tried different sectioning speeds, different thicknesses of the section and still continually only get a decent section every other turn. I was wondering if anyone has had any similar experiences or if maybe the machine just needs a maintenance check due to it being an older machine. I wasn't sure if the advancement mechanism may be off or if it is another issue that doesn't seem apparent to me. Any help would be greatly appreciated. Thanks, Elliott Perow Juniata College Biology POE CONFIDENTIALITY NOTICE: The materials in this electronic mail transmission (including all attachments) are private and confidential and are the property of the sender. The information contained in the material is privileged and is intended only for the use of the named addressee(s). If you are not the intended addressee, be advised that any unauthorized disclosure, copying, distribution or the taking of any action in reliance on the contents of this material is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender by replying to the e-mail, and then destroy it immediately. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------ Message: 16 Date: Thu, 3 Oct 2013 08:15:56 -0400 From: "Lee & Peggy Wenk" Subject: Re: [Histonet] HT HistoDeck question... To: "Stephenson, Sheryl" , Message-ID: <8FAB0141C65B42098CE4F110ECE9F372@HP2010> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original Personally, I think it's "a" is a wrong answer, and that you are correct that "b" is a better answer. My students and I have found a couple of other questions that we thought had the wrong answer indicated in the study set. Peggy A. Wenk, HTL(ASCP)SLS -----Original Message----- From: Stephenson, Sheryl Sent: Thursday, October 03, 2013 7:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HT HistoDeck question... Hi, Please clarify why this answer to the HistoDeck study question is a) and not b). Here is the question: 'Frozen section slides cut from fresh, unfixed tissue specimens are optimally fixed in which of the following solutions? a) 37%-40% formaldehyde b) Cold acetone c) Acetic acid alcohol d) Alcoholic formalin Thanks, Sheryl Stephenson | Histology Technician _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 17 Date: Thu, 3 Oct 2013 08:21:55 -0400 From: "Lee & Peggy Wenk" Subject: Re: [Histonet] RE: Inconsistent Sections with Cryostat To: "Manfre, Philip" , "Perow, Elliott S \(PEROWES10\)" , Message-ID: Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original I just attended Jan Minshew's workshop on cryostats at the NSH Symposium in Providence, RI, and she brought up something I had never thought of that causes thick and thin. If the handle that tightens the blade in the blade holder is over-tightened, the blade will become bowed, and that will cause thick-and-thin during sectioning. According to Jan, the handle should be tightened to be parallel to the angle of the blade holder "slope". A lot of times, the handle can be pushed further back towards the back of the cryostat, beyond being parallel with the slope. The thought seems to be, if tight is good, then tighter is better, but not in this case. Peggy A. Wenk, HTL(ASCP)SLS -----Original Message----- From: Manfre, Philip Sent: Thursday, October 03, 2013 7:54 AM To: Perow, Elliott S (PEROWES10) ; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Inconsistent Sections with Cryostat I believe you may need to have the unit serviced. It sounds like something is not tight enough, perhaps the stage or blade holder unit. You said you secured everything which makes me think you have some issue with the cryostat itself. If a sharp blade, tightened blade and specimen, and varying the speed doesn't work, then you may need professional assistance form a service technician, especially if it is an older unit that has been moved around. Have you tried different temperatures? I don't think that is the problem but it is worth a try. Good luck! Cryosectioning can be an art in itself. Those decent sections are likely thicker than you intend, by the way. Classic "thick and thin". Philip Manfre, B.A., HT (ASCP) Associate Principal Scientist Merck Research Laboratories WP45-251 PO Box 4 West Point, PA 19486 215-652-9750 215-993-0383 (fax) philip_manfre@merck.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Perow, Elliott S (PEROWES10) Sent: Thursday, October 03, 2013 12:33 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Inconsistent Sections with Cryostat Hello All, I have a Leica Cryocut 1800 with a Reichert-Jung 2020 microtome that has sat for 8 years and went through a move from one academic building to another. I am trying to section brook trout brain, but am having difficulty getting consistent sections. I will get a good section and then the next section will be a very very thin shaving of usually just the top portion of the OCT block. I have tried adjusting the clearance angle, made sure the blade and specimen is secured, replaced the blade with a new one, have tried different sectioning speeds, different thicknesses of the section and still continually only get a decent section every other turn. I was wondering if anyone has had any similar experiences or if maybe the machine just needs a maintenance check due to it being an older machine. I wasn't sure if the advancement mechanism may be off or if it is another issue that doesn't seem apparent to me. Any help would be greatly appreciated. Thanks, Elliott Perow Juniata College Biology POE CONFIDENTIALITY NOTICE: The materials in this electronic mail transmission (including all attachments) are private and confidential and are the property of the sender. The information contained in the material is privileged and is intended only for the use of the named addressee(s). If you are not the intended addressee, be advised that any unauthorized disclosure, copying, distribution or the taking of any action in reliance on the contents of this material is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender by replying to the e-mail, and then destroy it immediately. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 18 Date: Thu, 3 Oct 2013 09:40:06 -0400 From: "Watson, Linda" Subject: RE: [Histonet] HT HistoDeck question... To: Lee & Peggy Wenk , "Stephenson, Sheryl" , "histonet@lists.utsouthwestern.edu" Message-ID: <351A66CE7FB11D40AB8AC8FE5559EC7E11A975A411@ushpwbmsmmp008.one.ads.bms.com> Content-Type: text/plain; charset="us-ascii" For frozen cut sections, would the fixation also depend on what you plan on doing with it. For example, H&E, Special Stain or IHC? Please correct if I am wrong. I think that is a trick question!!! >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet- >bounces@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk >Sent: Thursday, October 03, 2013 8:16 AM >To: Stephenson, Sheryl; histonet@lists.utsouthwestern.edu >Subject: Re: [Histonet] HT HistoDeck question... > >Personally, I think it's "a" is a wrong answer, and that you are correct >that "b" is a better answer. My students and I have found a couple of >other >questions that we thought had the wrong answer indicated in the study >set. > >Peggy A. Wenk, HTL(ASCP)SLS >-----Original Message----- >From: Stephenson, Sheryl >Sent: Thursday, October 03, 2013 7:21 AM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] HT HistoDeck question... > >Hi, >Please clarify why this answer to the HistoDeck study question is a) >and >not b). > >Here is the question: > > 'Frozen section slides cut from fresh, unfixed tissue specimens are >optimally fixed in which of the following solutions? >a) 37%-40% formaldehyde >b) Cold acetone >c) Acetic acid alcohol >d) Alcoholic formalin > >Thanks, > > >Sheryl Stephenson | Histology Technician > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. ------------------------------ Message: 19 Date: Thu, 3 Oct 2013 13:42:34 +0000 From: Bernice Frederick Subject: RE: [Histonet] HT HistoDeck question... To: "Watson, Linda" , Lee & Peggy Wenk , "Stephenson, Sheryl" , "histonet@lists.utsouthwestern.edu" Message-ID: <62C639732D3F274DACED033EBDF6ADAF2F00D4EA@evcspmbx2.ads.northwestern.edu> Content-Type: text/plain; charset="us-ascii" We fix H&E's in 95% and our IHC protocol is acetone/alcohol fixation. Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Watson, Linda Sent: Thursday, October 03, 2013 8:40 AM To: Lee & Peggy Wenk; Stephenson, Sheryl; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HT HistoDeck question... For frozen cut sections, would the fixation also depend on what you plan on doing with it. For example, H&E, Special Stain or IHC? Please correct if I am wrong. I think that is a trick question!!! >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet- >bounces@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk >Sent: Thursday, October 03, 2013 8:16 AM >To: Stephenson, Sheryl; histonet@lists.utsouthwestern.edu >Subject: Re: [Histonet] HT HistoDeck question... > >Personally, I think it's "a" is a wrong answer, and that you are >correct that "b" is a better answer. My students and I have found a >couple of other questions that we thought had the wrong answer >indicated in the study set. > >Peggy A. Wenk, HTL(ASCP)SLS >-----Original Message----- >From: Stephenson, Sheryl >Sent: Thursday, October 03, 2013 7:21 AM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] HT HistoDeck question... > >Hi, >Please clarify why this answer to the HistoDeck study question is a) >and not b). > >Here is the question: > > 'Frozen section slides cut from fresh, unfixed tissue specimens are >optimally fixed in which of the following solutions? >a) 37%-40% formaldehyde >b) Cold acetone >c) Acetic acid alcohol >d) Alcoholic formalin > >Thanks, > > >Sheryl Stephenson | Histology Technician > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 20 Date: Thu, 3 Oct 2013 09:59:58 -0400 From: "Pam Barker" Subject: [Histonet] The NSH was AMAZING!!! Congratulations to the 2013 Leadership, Education and Advocacy Award Winners!! From Pam Barker and RELIA Solutions!! To: "Histonet" Message-ID: <006001cec040$d9e6e1f0$8db4a5d0$@earthlink.net> Content-Type: text/plain; charset="us-ascii" Hi Histonetters!!! How are you doing? The NSH was AMAZING!!!!! Providence was GORGEOUS!!!! The Speakers were BRILLIANT!!!! CONGRATULATIONS TO THE 2013 Leadership Education And Advocacy Award Winners: Histotechnologist of the Year-Wanda Jones J.B. McCormick Award- Peggy Wenk President's Award- Janet Dapson Lee G. Luna Foreign Travel Scholarship- M. Lamar Jones William Hacker Memorial Award- Anthony Wong Rosemary & Donald Ostermeier Memorial Award- Christiane Coady Ventana IHC Award- Tiana Baskin Biogenex Standardization in IHC Award-David Davis Biogenex Standardization in ISH Award Dale Telgenhoff Dako Standardization of IHC Award-Jennifer Wright Anne Preece Award -Sarah Mack Leica Leadership in Management Award-Nirmala Amin Leica Leadership in Teaching Award- Elaine Basham Newcomer Helping Hand Award- Jean Mitchell Epitomics IHC Award- Melissa Downing Jules Elias Excellence in IHC Award- James Burchette Since I have returned to the office my phone has been ringing off the hook with exciting new opportunities. Here is a list of my current openings. All of these are full time permanent positions. My clients offer excellent compensation, benefits and relocation assistance. And they are ready to interview and hire right away! HISTOLOGY SUPERVISORS/MANAGERS: Histology Supervisor - Tallahassee, FL Histology Supervisor - West Palm Beach, FL Histology Supervisor - Seattle, WA Lead Pathology Tech - East of Columbus, OH HISTOTECHNICIANS/HISTOTECHNOLOGISTS: Histology Tech with Electron Microscopy - Southern CA Histotechnician Day shift -East of Columbus, OH Histotechnician Day shift - Tyler, TX Histotechnologist - Days - Seattle, WA Histology Tech - West Palm Beach, FL Histotechnician - Days - Harrisonburg, VA Histotechnician - Nights - Nashville, TN Senior Histotechnologist with FISH - Louisville, KY OTHER OPPORTUNITIES MT (ASCP) with Molecular experience- RTP, SC Of course I can't put all the information about these opportunities in an e-mail. So if you or anyone you know might be interested in hearing more about any of these positions or want help with a job search in another area please contact me. I can be reached at 866-607-3542 or relia1@earthlink.net. Remember it never hurts to look. Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 119, Issue 4 **************************************** Charles Sturt University | ALBURY-WODONGA | BATHURST | CANBERRA | DUBBO | GOULBURN | MELBOURNE | ONTARIO | ORANGE | PORT MACQUARIE | SYDNEY | WAGGA WAGGA | LEGAL NOTICE This email (and any attachment) is confidential and is intended for the use of the addressee(s) only. If you are not the intended recipient of this email, you must not copy, distribute, take any action in reliance on it or disclose it to anyone. Any confidentiality is not waived or lost by reason of mistaken delivery. Email should be checked for viruses and defects before opening. Charles Sturt University (CSU) does not accept liability for viruses or any consequence which arise as a result of this email transmission. Email communications with CSU may be subject to automated email filtering, which could result in the delay or deletion of a legitimate email before it is read at CSU. The views expressed in this email are not necessarily those of CSU. Charles Sturt University in Australia http://www.csu.edu.au The Grange Chancellery, Panorama Avenue, Bathurst NSW Australia 2795 (ABN: 83 878 708 551; CRICOS Provider Numbers: 00005F (NSW), 01947G (VIC), 02960B (ACT)). TEQSA Provider Number: PV12018 Charles Sturt University in Ontario http://www.charlessturt.ca 860 Harrington Court, Burlington Ontario Canada L7N 3N4 Registration: www.peqab.ca Consider the environment before printing this email. Disclaimer added by CodeTwo Exchange Rules 2007 http://www.codetwo.com From turkekul <@t> gmail.com Thu Oct 3 17:59:51 2013 From: turkekul <@t> gmail.com (Mesru T) Date: Thu Oct 3 17:59:56 2013 Subject: [Histonet] HistoDeck questions Message-ID: HistoDeck questions are companion of the Carson' s book. Carson fixes in 37-40% formaldehyde for OilRed O. Any acetone or alcohol will dissolve the fat and OilRedO will be negative or suboptimum. The answer should be "A" Mesru From melibur <@t> msn.com Thu Oct 3 19:17:14 2013 From: melibur <@t> msn.com (melissa b) Date: Thu Oct 3 19:17:21 2013 Subject: [Histonet] Dacie Iron Stain Message-ID: Hi Everyone, this is my first time posting, hope this works. One of our pathologists is interested in the Dacie iron stain for bone marrow specimens. Where can I purchase this stain or is this simply a method? I greatly appreciate your help! Melissa Anatomic Pathology Supervisor Nemours Children's Hospital From Tony_Reilly <@t> health.qld.gov.au Thu Oct 3 19:32:26 2013 From: Tony_Reilly <@t> health.qld.gov.au (Tony Reilly) Date: Thu Oct 3 19:32:56 2013 Subject: [Histonet] Bodian In-Reply-To: <524D9D770200007A0002721B@gatedom1.upstate.edu> References: <524D9D770200007A0002721B@gatedom1.upstate.edu> Message-ID: <524E993A.411C.0039.0@health.qld.gov.au> Hi Helene Have you tried a Bielschowsky instead. It is widely reported to provide better staining than the Bodian. http://www.ncbi.nlm.nih.gov/pubmed/2422580 regards Tony Tony Reilly B.App.Sc. , M.Sc. Chief Scientist, Anatomical Pathology Pathology Queensland-PA Laboratory ________________________________________________ Health Services Support Agency | Department of Health Level 1, Building 15,Princess Alexandra Hospital Ipswich Road,WOOLLOONGABBA Qld4102 Ph: 07 3176 2412 Mob: 0402 139411 Fax: 07 3176 2930 Email: tony_reilly@health.qld.gov.au Web: www.health.qld.gov.au/qhcss/ >>> "Helene Degan" 10/4/2013 6:38 am >>> Hi Thanks to every one that sent me Acid Phosphotase procedure for muscle biopsies. I also need some help with the bodian stain, we are not getting any staining on the slides, we have a procedure that they used years ago and now we can't get it working (we did purchase new chemicals) and we also tried a stain kit with very little results. Thanks again Helene Upstate Medical University _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ********************************************************************************** From subash.govender <@t> uct.ac.za Fri Oct 4 00:22:03 2013 From: subash.govender <@t> uct.ac.za (Subash Govender) Date: Fri Oct 4 00:22:22 2013 Subject: [Histonet] RE: Microtomes Message-ID: <32CD6307BAB24F4DAD1C6E6B3E8AA22349426D66@SRVWINEXC004.wf.uct.ac.za> Hi there We recently purchased a Leica microtome and all seems to be going very well. It cuts beautifully. Just warn students not to clean the machine with xylene as ours did and smudged the writing on the microtome. Good luck Subash Govender Anatomical Pathology Research Lab University of Cape Town Medical School South Africa Email: subash.govender@uct.ac.za ________________________________ UNIVERSITY OF CAPE TOWN This e-mail is subject to the UCT ICT policies and e-mail disclaimer published on our website at http://www.uct.ac.za/about/policies/emaildisclaimer/ or obtainable from +27 21 650 9111. This e-mail is intended only for the person(s) to whom it is addressed. If the e-mail has reached you in error, please notify the author. If you are not the intended recipient of the e-mail you may not use, disclose, copy, redirect or print the content. If this e-mail is not related to the business of UCT it is sent by the sender in the sender's individual capacity. From ruppert.amysue <@t> marshfieldclinic.org Fri Oct 4 00:55:59 2013 From: ruppert.amysue <@t> marshfieldclinic.org (Ruppert, Amysue) Date: Fri Oct 4 00:56:18 2013 Subject: [Histonet] HT HistoDeck question...&In-Reply-To=9F3CFEE76E51B64991C7485270890B40 Message-ID: <201310040555.r945m8BQ021923@mailhost2.mfldclin.edu> Please see Freida Carson's Histotechnology-A self Instructional text (2nd edition pg 98, or 3rd edition pps 117-118). There you will find that the correct answer is "A"- 37-40 % formaldehyde. In the Notes section she explains "Many laboratories use acetone or alcohol as the fixative for frozen section; however, concentrated formaldehyde yields morphologic preservation more like that seen in permanent sections. Fixation in alcoholic formalin is also good" Since the question asks for "optiomally" fixed, the correct answer would be "A" and not "D" hope this helps. AmySue Ruppert,HT(ASCP) MB(ASCP) Marshfield Labs,Histology ______________________________________________________________________ The contents of this message may contain private, protected and/or privileged information. If you received this message in error, you should destroy the e-mail message and any attachments or copies, and you are prohibited from retaining, distributing, disclosing or using any information contained within. Please contact the sender and advise of the erroneous delivery by return e-mail or telephone. Thank you for your cooperation. From SAllen <@t> dsmanitoba.ca Fri Oct 4 08:31:18 2013 From: SAllen <@t> dsmanitoba.ca (Sharon Allen) Date: Fri Oct 4 08:31:24 2013 Subject: [Histonet] AedestaT-cell/tissue preservation (ACTP) media for muscle bx's Message-ID: <092377D5AD912F4AA4241526CE3DC7B4010D1D@REG1MSMX0023.ad.wrha.mb.ca> I had read the article on: Nonfrozen Transport Medium Preserves and Restores Skeletal Muscle Enzymatic Activity and Morphology from the The Journal of Histotechnology / Vol. 32, No. 2 / June 2009 posted lately on the Histonet. I was wondering if anyone had used or heard of any further testing on the ACTP media for restoring & preserving frozen muscle bx's. I have a whole year of muscle bx's from the mid 90's with severe freezing artifact, unfortunately someone shut our -70?C freezer off instead of the alarm, on a Friday afternoon & was not discovered until Mon. morning. If anyone has any information I would appreciate it. It would be great to be able to restore these muscles when needed. Thank you, Sharon Allen Senior Medical Technologist Neuropathology Lab-MS435U Health Sciences Centre 820 Sherbrook Street Winnipeg,MB, CA R3A 1R9 e-mail: sallen@dsmanitoba.ca This email and/or any documents in this transmission is intended for the addressee(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original. Ce courriel et tout document dans cette transmission est destin? ? la personne ou aux personnes ? qui il est adress?. Il peut contenir des informations privil?gi?es ou confidentielles. Toute utilisation, divulgation, distribution, copie, ou diffusion non autoris?e est strictement d?fendue. Si vous n'?tes pas le destinataire de ce message, veuillez en informer l'exp?diteur imm?diatement et lui remettre l'original. From kgrobert <@t> rci.rutgers.edu Fri Oct 4 09:08:37 2013 From: kgrobert <@t> rci.rutgers.edu (kgrobert@rci.rutgers.edu) Date: Fri Oct 4 09:08:43 2013 Subject: [Histonet] Processing thin brain slices in. Histogel Message-ID: <0402891d325361897e673bfcaadfb7bf.squirrel@webmail.rci.rutgers.edu> We are trying to process thin slices of mouse cerebellum embedded in Histogel into paraffin, and we have encountered two problems: 1) the Histogel button with the slices inside it has been curling up in the processor ( before anyone asks, we're using our own cassettes, not the histoscreen ones that come in that starter kit), and pressing it flat with weights while embedding puts stress on the tissue....has anyone else seen this? 2) The Histogel, once embedded in paraffin, has occasionally chipped out of the block on sectioning. This, to me, implies an infiltration problem, but this stuff is supposed to be compatible with paraffin processing. Why would it not infiltrate? We ran the buttons on the biopsy program that came with our VIP 5. If you want details, I'll send them once the processor is done. The Histogel is still within its expiration date, and the curly batches were done with a fresh tube of Histogel from the fridge. Any and all ideas/suggestions/solutions are most welcome. Happy Friday, Kathleen Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology & Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (848) 445-1443 FAX (732) 445-6905 From rjbuesa <@t> yahoo.com Fri Oct 4 09:35:10 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Oct 4 09:35:15 2013 Subject: [Histonet] Bodian In-Reply-To: <524D9D770200007A0002721B@gatedom1.upstate.edu> References: <524D9D770200007A0002721B@gatedom1.upstate.edu> Message-ID: <1380897310.1472.YahooMailNeo@web163101.mail.bf1.yahoo.com> The fundamental problem with the Bodian stain is the silver protargol. Once you open your expensive bottle (only very few manufacturers of the powder) it starts to oxidize end in only a few days it is no longer able to react properly. That is why I developed a procedure where you prepare your own silver protargol FRESH before every procedure. This, although sound difficult, it is not. Under separate cover I am sending you a publication I did on this method and I am sure you will overcome all your difficulties. Ren? J. ? ________________________________ From: Helene Degan To: histonet@lists.utsouthwestern.edu Sent: Thursday, October 3, 2013 4:38 PM Subject: [Histonet] Bodian Hi Thanks to every one that sent me Acid Phosphotase procedure for muscle biopsies. I also need some help with the bodian stain, we are not? getting any staining on the slides, we have a procedure that they used years ago and now we can't get it working (we did purchase new chemicals) and we also tried a stain kit with very little results. Thanks again Helene Upstate Medical University _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 <@t> earthlink.net Fri Oct 4 10:14:00 2013 From: relia1 <@t> earthlink.net (Pam Barker) Date: Fri Oct 4 10:14:07 2013 Subject: [Histonet] RELIA Solutions Special Career Opportunity Alert Exclusive for Laboratory Directors and Managers !!! 10-03-2013 Message-ID: <035f01cec114$5b4ef150$11ecd3f0$@earthlink.net> Hello Histonetters!! TGIF!!! RELIA has been engaged by an acute care hospital in beautiful Southern CA (near San Diego) to assist in their search for a director of laboratory services. Previous experience in the role of laboratory management in an acute care setting is essential. Our client offers an excellent compensation and relocation package. If you would like more information or know of someone else who might be interested, please contact me at relia1@earthlink.net or 866-607-3542. I am available to discuss the opportunity at your convenience including after hours. Thanks-Pam All inquiries will be kept in the strictest of confidence and handled with the utmost discretion. Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From Ronald.Houston <@t> nationwidechildrens.org Fri Oct 4 10:16:31 2013 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Fri Oct 4 10:16:37 2013 Subject: [Histonet] Endogen Message-ID: Does anyone know if Endogen has gone out of business or have they been gobbled up by another larger conglomerate? Trying to get one of their antibodies and can no longer find their web-site Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org From Valerie.Hannen <@t> parrishmed.com Fri Oct 4 10:18:51 2013 From: Valerie.Hannen <@t> parrishmed.com (Hannen, Valerie) Date: Fri Oct 4 10:18:54 2013 Subject: [Histonet] Microtome steel blade holder Message-ID: <450B7A81EDA0C54E97C53D60F00776C3232B4BB667@isexstore03> Good Morning all... I am having a problem with my Microtome steel blade holder. One of the screws on the blade block that helps hold the blade tightly clamped has "stripped" itself out. I am wondering if anyone in out there in Histo-land who no longer is using steel blades, but still has the knife block that is used with steel blades, might be willing to part with it. I have a Leica 2025. Thanks ever so much!! Any and all help is greatly appreciated. Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.hannen@parrishmed.com =================== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" =================== From flnails <@t> texaschildrens.org Fri Oct 4 10:29:36 2013 From: flnails <@t> texaschildrens.org (Nails, Felton) Date: Fri Oct 4 10:30:12 2013 Subject: [Histonet] Body bags usage. Message-ID: <327E034F1892504289B7A17EC71DF9F303DD14@TCFMSG03.ad.texaschildrenshospital.org> When you have a patient that expires, do you require that the bodies be brought down in body bags for an autopsy or funeral home pick up? ______________________________________________________________________ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ______________________________________________________________________ From koellingr <@t> comcast.net Fri Oct 4 10:38:20 2013 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Fri Oct 4 10:38:42 2013 Subject: [Histonet] Endogen In-Reply-To: References: Message-ID: <1501943779.42749.1380901100050.JavaMail.root@comcast.net> I recently had the same question. They were gobbled. Try Endogen antibodies at Thermo Scientific conglomerate. Ray Seattle, WA ----- Original Message ----- From: "Ronald Houston" To: histonet@lists.utsouthwestern.edu Sent: Friday, October 4, 2013 8:16:31 AM Subject: [Histonet] Endogen Does anyone know if Endogen has gone out of business or have they been gobbled up by another larger conglomerate? Trying to get one of their antibodies and can no longer find their web-site Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From barbara.tibbs <@t> accuratediagnosticlabs.com Fri Oct 4 11:49:28 2013 From: barbara.tibbs <@t> accuratediagnosticlabs.com (Barbara Tibbs) Date: Fri Oct 4 11:49:59 2013 Subject: [Histonet] RE: Body bags usage. In-Reply-To: <327E034F1892504289B7A17EC71DF9F303DD14@TCFMSG03.ad.texaschildrenshospital.org> References: <327E034F1892504289B7A17EC71DF9F303DD14@TCFMSG03.ad.texaschildrenshospital.org> Message-ID: <90731ace7f1b4b628ea56f03a349be37@BL2PR04MB196.namprd04.prod.outlook.com> I don't work in a hospital now but when I did, on and off, for the past 30 years or so a body was always brought down to the morgue in a body bag or shroud. If a patient was morbidly obese the body was placed in a bariatric body bag and brought to the morgue. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.tibbs@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Nails, Felton Sent: Friday, October 04, 2013 1:29 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Body bags usage. When you have a patient that expires, do you require that the bodies be brought down in body bags for an autopsy or funeral home pick up? ______________________________________________________________________ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From robinsoc <@t> mercyhealth.com Fri Oct 4 12:09:30 2013 From: robinsoc <@t> mercyhealth.com (Cynthia Robinson) Date: Fri Oct 4 12:09:39 2013 Subject: [Histonet] Hep Par 1 validation Message-ID: <524EAFFA020000AF000101AD@nodcdmg2.no.trinity-health.org> I am wondering if anyone has hepatoblastoma....either in liver or metastatic tumor....in their tissue bank. I am working up Hep Par 1 and have a few cases but would like a few more. We are working up this antibody to use in metastatic case with unknown primary as a way to rule out possibilities. My pathologist would really like to see a case with tumor surrounded by normal liver, if possible. If you can help please contact me. I have control blocks I would be willing to trade. Thanks.....Cindi Cindi Robinson HT(ASCP) Mercy Medical Center Dunes Medical Laboratories 350 W Anchor Dr Dakota Dunes SD 57049 phone-712-279-2768 robinsoc@mercyhealth.com From Richard.Cartun <@t> hhchealth.org Fri Oct 4 12:24:00 2013 From: Richard.Cartun <@t> hhchealth.org (Cartun, Richard) Date: Fri Oct 4 12:24:13 2013 Subject: [Histonet] Hep Par 1 validation In-Reply-To: <524EAFFA020000AF000101AD@nodcdmg2.no.trinity-health.org> References: <524EAFFA020000AF000101AD@nodcdmg2.no.trinity-health.org> Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E01887DEF@HHCEXCHMB05.hhcsystem.org> FYI - be careful; other cancers besides HCC can show immunoreactivity for Hepatocyte antigen (clone OCH1E5). Arginase (rabbit mAb) is another marker that can be used for HCC and it is more specific than Hepatocyte antigen. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax richard.cartun@hhchealth.org ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Cynthia Robinson [robinsoc@mercyhealth.com] Sent: Friday, October 04, 2013 1:09 PM To: histonet Subject: [Histonet] Hep Par 1 validation I am wondering if anyone has hepatoblastoma....either in liver or metastatic tumor....in their tissue bank. I am working up Hep Par 1 and have a few cases but would like a few more. We are working up this antibody to use in metastatic case with unknown primary as a way to rule out possibilities. My pathologist would really like to see a case with tumor surrounded by normal liver, if possible. If you can help please contact me. I have control blocks I would be willing to trade. Thanks.....Cindi Cindi Robinson HT(ASCP) Mercy Medical Center Dunes Medical Laboratories 350 W Anchor Dr Dakota Dunes SD 57049 phone-712-279-2768 robinsoc@mercyhealth.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From turkekul <@t> gmail.com Fri Oct 4 12:37:08 2013 From: turkekul <@t> gmail.com (Mesru T) Date: Fri Oct 4 12:37:12 2013 Subject: [Histonet] HistoDeck question Message-ID: I have 2nd and 3rd edition of Freida Carson's Histotechnology-A self Instructional text book. When I read the : 2nd edition pg 98, or 3rd edition pps 117-118. I noticed that this is not for OilRed O staining but for forozen sections in general. When I read: Freida Carson's Histotechnology-A self Instructional text (2nd edition pps 151-152, or 3rd edition pps 184-185, I found out that Carson warns that no alcoholic fixative should be used for Oil Red O staining. Only Formaldehyde (10% formalin or 37% formaldehyde) or calcium-formol are suggested to be used. Carson also warns that any solution containing alcohol or organic solvents should be used with caution. She also mentions that isopropyl alcohol (IPA) or propylene glycol are safer and do not dissolve lipids as much. I personally also try to avoid using hematoxylin that contains alcohol. Correct Answer is A and all others are incorrect. Regards, Mesru From Cathie.Crukley <@t> lhsc.on.ca Fri Oct 4 14:11:00 2013 From: Cathie.Crukley <@t> lhsc.on.ca (Cathie Crukley) Date: Fri Oct 4 14:11:15 2013 Subject: [Histonet] Finetec Cryofilm? Message-ID: <524EDA84020000BF0002A802@lhscgwiao.lhsc.on.ca> There was an earlier email from Elizabeth Cameron looking for information on cryofilm. We have been purchasing cryofilm from a company called Section-Lab in Japan. She would be able to get information on their products from info@section-lab.jp Cathie Crukley London, Ontario -------------------------------------------------------------------------------- This information is directed in confidence solely to the person named above and may contain confidential and/or privileged material. This information may not otherwise be distributed, copied or disclosed. If you have received this e-mail in error, please notify the sender immediately via a return e-mail and destroy original message. Thank you for your cooperation. From brentrankin <@t> rankinbiomed.com Fri Oct 4 14:17:40 2013 From: brentrankin <@t> rankinbiomed.com (Brent Rankin) Date: Fri Oct 4 14:17:46 2013 Subject: [Histonet] Re: Microtome steel blade holder Message-ID: Hi Valerie, I have a couple different knife holders for the 20 series microtomes. Just making sure we are on the same page, you have a standard knife holder and not a disposable blade holder? If you can send me a picture of which one you have I might be able to help you out. Regards, Brent Rankin VP/ Business Development *Rankin Biomedical Corporation* *www.RankinBiomed.com* *brentrankin@rankinbiomed.com* 14515 Mackey Rd. Holly, MI 48442 (877) 882-3679 Toll Free (248) 625-1070 Fax Good Morning all... I am having a problem with my Microtome steel blade holder. One of the screws on the blade block that helps hold the blade tightly clamped has "stripped" itself out. I am wondering if anyone in out there in Histo-land who no longer is using steel blades, but still has the knife block that is used with steel blades, might be willing to part with it. I have a Leica 2025. Thanks ever so much!! Any and all help is greatly appreciated. Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.hannen@parrishmed.com From ander093 <@t> umn.edu Fri Oct 4 14:58:24 2013 From: ander093 <@t> umn.edu (LuAnn Anderson) Date: Fri Oct 4 14:58:30 2013 Subject: [Histonet] Bodian In-Reply-To: <1380897310.1472.YahooMailNeo@web163101.mail.bf1.yahoo.com> References: <524D9D770200007A0002721B@gatedom1.upstate.edu> <1380897310.1472.YahooMailNeo@web163101.mail.bf1.yahoo.com> Message-ID: <524F1DE0.1070501@umn.edu> Rene............could I please get a copy of that procedure ? I would greatly appreciate it. On 10/4/2013 9:35 AM, Rene J Buesa wrote: > The fundamental problem with the Bodian stain is the silver protargol. Once you open your expensive bottle (only very few manufacturers of the powder) it starts to oxidize end in only a few days it is no longer able to react properly. > That is why I developed a procedure where you prepare your own silver protargol FRESH before every procedure. This, although sound difficult, it is not. Under separate cover I am sending you a publication I did on this method and I am sure you will overcome all your difficulties. > Ren? J. > > > > ________________________________ > From: Helene Degan > To: histonet@lists.utsouthwestern.edu > Sent: Thursday, October 3, 2013 4:38 PM > Subject: [Histonet] Bodian > > > Hi > Thanks to every one that sent me Acid Phosphotase procedure for muscle biopsies. I also need some help with the bodian stain, we are not getting any staining on the slides, we have a procedure that they used years ago and now we can't get it working (we did purchase new chemicals) and we also tried a stain kit with very little results. > > Thanks again > > Helene > Upstate Medical University > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sdysart <@t> mirnarx.com Fri Oct 4 15:02:07 2013 From: sdysart <@t> mirnarx.com (Sarah Dysart) Date: Fri Oct 4 15:02:14 2013 Subject: [Histonet] NSH emails Message-ID: <9b3925813a564bb6a750e397f3104e61@BY2PR07MB106.namprd07.prod.outlook.com> Does anyone know how to get an email address of someone who presented? If not does anyone have the addresses for Melanie von Brandenstein or Heike Goebel? Thanks Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 From cathy.crumpton <@t> tuality.org Fri Oct 4 15:18:14 2013 From: cathy.crumpton <@t> tuality.org (Cathy Crumpton) Date: Fri Oct 4 15:18:27 2013 Subject: [Histonet] lower glands in colon not staining with IHC Message-ID: On our AE1/AE3 and CK 20 slides we are noticing a deminished staining in the lower glands of the colon sections. My first thought was inadequate fixation, however the BerEP4 stained evenly. This has been happening on different tissues, including small biopsies. Does anyone have any ideas as to something else that could be causing a lack of staining on the internal colon portions? We use Dako reagents and polymer kit and the skin and other tissues in the sausage block stain beautifully. Cathy Crumpton HT(ASCP), Lead Histotechnician Tuality Community Hospital 503-681-1292 From cshnewsletter <@t> gmail.com Fri Oct 4 16:00:03 2013 From: cshnewsletter <@t> gmail.com (CSH Newsletter) Date: Fri Oct 4 16:00:15 2013 Subject: [Histonet] Kawamoto's film method Message-ID: <188B4088-82AD-46C7-8E8B-B758FC271289@gmail.com> Hi Everyone, I'm trying to work out sectioning frozen undecalcified mouse bone marrow without using the CryoJane method. The UV light interferes with protease activity. I've been reading about the Kawamoto method and wanted to know people's opinion and how to get hold of the tape. Thanks in advance for your replies. Judi Ford Cytomx Therapeutics From lpwenk <@t> sbcglobal.net Fri Oct 4 17:22:12 2013 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Fri Oct 4 17:22:18 2013 Subject: [Histonet] NSH emails In-Reply-To: <9b3925813a564bb6a750e397f3104e61@BY2PR07MB106.namprd07.prod.outlook.com> References: <9b3925813a564bb6a750e397f3104e61@BY2PR07MB106.namprd07.prod.outlook.com> Message-ID: <23BCD315DD9E4A59967E5675236BA592@HP2010> 1. Convention booklet - At the convention, every attendee was given a spiral bound booklet, with thick glossy pages. The last tab is the Speaker Directory with Hospital/lab info, city/state, email addresses. 2. NSH Member Directory - go to NSH webpage (www.nsh.org ), go to Member Directory - right side, half way down page. Must be NSH member, and if you haven't registered a user name and password, need to do that. Can get addresses, phone numbers and email addresses if they are NSH members. 3. Call NSH Office - 443-535-4060 Peggy A. Wenk, HTL(ASCP)SLS -----Original Message----- From: Sarah Dysart Sent: Friday, October 04, 2013 4:02 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] NSH emails Does anyone know how to get an email address of someone who presented? If not does anyone have the addresses for Melanie von Brandenstein or Heike Goebel? Thanks Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From epchatfield <@t> ihis.org Mon Oct 7 04:58:58 2013 From: epchatfield <@t> ihis.org (Elizabeth Chatfield) Date: Mon Oct 7 04:59:08 2013 Subject: [Histonet] CD117 Message-ID: <52525BB2020000BC0006085C@gwia-out.peigov> Hi Folks, I'm looking for a new vendor for CD117. Up until now we purchased from Leica, they no longer produce it. Elizabeth QEH Charlottetown, PE ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. From trathborne <@t> somerset-healthcare.com Mon Oct 7 07:13:57 2013 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Mon Oct 7 07:15:06 2013 Subject: [Histonet] CD117 In-Reply-To: <52525BB2020000BC0006085C@gwia-out.peigov> References: <52525BB2020000BC0006085C@gwia-out.peigov> Message-ID: <3AD061FE740D464FAC7BF6B5CFB75707A8A82E2B@smcmail02.somerset-healthcare.com> We use Dako's polyclonal on our Bond III, diluted 1:400, catalog A4502. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chatfield Sent: Monday, October 07, 2013 5:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CD117 Hi Folks, I'm looking for a new vendor for CD117. Up until now we purchased from Leica, they no longer produce it. Elizabeth QEH Charlottetown, PE ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. From LSebree <@t> uwhealth.org Mon Oct 7 07:36:10 2013 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Mon Oct 7 07:36:18 2013 Subject: [Histonet] CD117 In-Reply-To: <52525BB2020000BC0006085C@gwia-out.peigov> References: <52525BB2020000BC0006085C@gwia-out.peigov> Message-ID: <77DD817201982748BC67D7960F2F76AF06C93D@UWHC-MBX12.uwhis.hosp.wisc.edu> Elizabeth, We get a rabbit monoclonal from Cell Marque, cat. # 117R, clone YR145 that we are happy with. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chatfield Sent: Monday, October 07, 2013 4:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CD117 Hi Folks, I'm looking for a new vendor for CD117. Up until now we purchased from Leica, they no longer produce it. Elizabeth QEH Charlottetown, PE ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. From BergerR1 <@t> email.chop.edu Mon Oct 7 08:53:27 2013 From: BergerR1 <@t> email.chop.edu (Berger, Rebecca) Date: Mon Oct 7 08:53:32 2013 Subject: [Histonet] Masson's Trichrome Message-ID: <5A590EB108038B4FA01F8CDB39C6541B012E8B64@EXCMBXPW7.chop.edu> Hi Histonet, Quick question about Masson's Trichrome. I'm staining several batches of slides by hand, and normally only use the phosphotungstic/phosphomolybdic acid solution, as well as the 1% acetic acid solution, just once and replace it each time I do a stain. Does anyone out there re-use the acid solution? It seems like a real waste (in many ways) to replace it each time I do the stain but I've yet to experiment with re-using them. Thanks for your help in advance, Becky Rebecca Berger, HT(ASCP)CM Research Technician Division of Orthopedic Surgery The Children's Hospital of Philadelphia Research Institute 3615 Civic Center Boulevard, ARC904 (Lab) Philadelphia, PA 19104 Phone: 267-425-2076 Email: bergerr1@email.chop.edu From Richard.Cartun <@t> hhchealth.org Mon Oct 7 09:13:29 2013 From: Richard.Cartun <@t> hhchealth.org (Cartun, Richard) Date: Mon Oct 7 09:13:36 2013 Subject: [Histonet] CD117 In-Reply-To: <52525BB2020000BC0006085C@gwia-out.peigov> References: <52525BB2020000BC0006085C@gwia-out.peigov> Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E01888021@HHCEXCHMB05.hhcsystem.org> In early 2012 we started using Cell Marque's rabbit mAb (clone YR145) for CD117 detection and we are very happy with it. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax richard.cartun@hhchealth.org ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Elizabeth Chatfield [epchatfield@ihis.org] Sent: Monday, October 07, 2013 5:58 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CD117 Hi Folks, I'm looking for a new vendor for CD117. Up until now we purchased from Leica, they no longer produce it. Elizabeth QEH Charlottetown, PE ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From CObregon <@t> mhs.net Mon Oct 7 09:32:36 2013 From: CObregon <@t> mhs.net (Obregon, Cecilia) Date: Mon Oct 7 09:32:51 2013 Subject: [Histonet] CD117 In-Reply-To: <52525BB2020000BC0006085C@gwia-out.peigov> References: <52525BB2020000BC0006085C@gwia-out.peigov> Message-ID: <598BB4A3A92D7F4DAA14C30E4AE70C2F27BB5F81@MHSEXMB05.mhs.net> We have been using the CD117 Rabbit Polyclonal from Dako (1:150) for many years now, and it works very well on our Ventana platform. Haven't had any problems with this antibody. Thank you, Cecilia M. Obregon Memorial Regional Hospital 3501 Johnson Street Hollywood, FL 33029 954-265-5317 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Elizabeth Chatfield [epchatfield@ihis.org] Sent: Monday, October 07, 2013 5:58 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CD117 Hi Folks, I'm looking for a new vendor for CD117. Up until now we purchased from Leica, they no longer produce it. Elizabeth QEH Charlottetown, PE ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. From Jonathan.Cremer <@t> med.kuleuven.be Mon Oct 7 09:32:40 2013 From: Jonathan.Cremer <@t> med.kuleuven.be (Jonathan Cremer) Date: Mon Oct 7 09:32:53 2013 Subject: [Histonet] RE: Masson's Trichrome In-Reply-To: <5A590EB108038B4FA01F8CDB39C6541B012E8B64@EXCMBXPW7.chop.edu> References: <5A590EB108038B4FA01F8CDB39C6541B012E8B64@EXCMBXPW7.chop.edu> Message-ID: The PMA-PTA can be reused, even when the color changes to green. I don't see a problem in using fresh 1% acetic acid with every batch of slides? I just make up a 1l bottle, or mix it quickly when doing the stain. Glacial AA isn't that expensive, and it's no problem to pour down the drain (household vinegar is more concentrated). Unless you have to hang on to every single molecule of dye stuff and have it hauled off by a waste company... --- Jonathan Cremer Laboratory Technician TARGID - KU Leuven Gasthuisberg CDG; Labo Experimentele Immunologie; Herestraat 49 bus 811; 3000 Leuven; Belgium ________________________________________ Van: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] namens Berger, Rebecca [BergerR1@email.chop.edu] Verzonden: maandag 7 oktober 2013 15:53 Aan: histonet@lists.utsouthwestern.edu Onderwerp: [Histonet] Masson's Trichrome Hi Histonet, Quick question about Masson's Trichrome. I'm staining several batches of slides by hand, and normally only use the phosphotungstic/phosphomolybdic acid solution, as well as the 1% acetic acid solution, just once and replace it each time I do a stain. Does anyone out there re-use the acid solution? It seems like a real waste (in many ways) to replace it each time I do the stain but I've yet to experiment with re-using them. Thanks for your help in advance, Becky Rebecca Berger, HT(ASCP)CM Research Technician Division of Orthopedic Surgery The Children's Hospital of Philadelphia Research Institute 3615 Civic Center Boulevard, ARC904 (Lab) Philadelphia, PA 19104 Phone: 267-425-2076 Email: bergerr1@email.chop.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Mon Oct 7 09:50:57 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Oct 7 10:00:55 2013 Subject: [Histonet] Masson's Trichrome In-Reply-To: <5A590EB108038B4FA01F8CDB39C6541B012E8B64@EXCMBXPW7.chop.edu> References: <5A590EB108038B4FA01F8CDB39C6541B012E8B64@EXCMBXPW7.chop.edu> Message-ID: <1381157457.28835.YahooMailNeo@web163102.mail.bf1.yahoo.com> Just make a quick calculation of how much "diluted" is the cost of the reagent and you will realize that re-using the solutions? is not worth it from the monetary point of view, and much less from the quality aspect. Do not reuse them. Ren? J. ________________________________ From: "Berger, Rebecca" To: "histonet@lists.utsouthwestern.edu" Sent: Monday, October 7, 2013 9:53 AM Subject: [Histonet] Masson's Trichrome Hi Histonet, Quick question about Masson's Trichrome. I'm staining several batches of slides by hand, and normally only use the phosphotungstic/phosphomolybdic acid solution, as well as the 1% acetic acid solution, just once and replace it each time I do a stain.? Does anyone out there re-use the acid solution? It seems like a real waste (in many ways) to replace it each time I do the stain but I've yet to experiment with re-using them. Thanks for your help in advance, Becky Rebecca Berger, HT(ASCP)CM Research Technician Division of Orthopedic Surgery The Children's Hospital of Philadelphia Research Institute 3615 Civic Center Boulevard, ARC904 (Lab) Philadelphia, PA 19104 Phone: 267-425-2076? Email: bergerr1@email.chop.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histo <@t> pathlab.us Mon Oct 7 10:26:16 2013 From: histo <@t> pathlab.us (Histology) Date: Mon Oct 7 10:26:47 2013 Subject: [Histonet] salt split Message-ID: <9C0B151B30AC5F4ABFD1B69D915F84CF33ED4E@dc01.DominionPath.local> Hi all- Has anyone had problems with the salt split method for DIFs not separating? It has been sitting in 1M NaCl for 4 days now and is still not budging. We do not do this method often and any suggestions would be greatly appreciated. Thanks, Mehndi Helgren Dominion Pathology Laboratories 733 Boush St. Suite 200 Norfolk, VA 23510 757-664-7901 histo@pathlab.us From ruio7 <@t> hotmail.com Mon Oct 7 10:29:19 2013 From: ruio7 <@t> hotmail.com (Rui TAHARA) Date: Mon Oct 7 10:29:27 2013 Subject: [Histonet] Undecalcified sample in paraffin and plastic media In-Reply-To: References: , <1C480BD1-5897-4998-8F58-F0636C015271@hotmail.com>, Message-ID: Thank you for your information. Since in our lab we have never used MMA and also no vacuum I decided to ask histological service to embed the sample.I will section it and stain them with Von kossa/Alcian blue by myself. The sample is being processed now and i will see if the images of sample embeded in MMA would work better than the paraffin one for my project. The technician told me its hard to obtain a good section embedded in MMA compared to paraffin. Would you give me some tips or protocol for MMA sectioning? Do i need to use adhesive to place the MMA section on the slide? In general does the staining time for paraffin sample work for MMA sample? Thank you, Rui Date: Wed, 2 Oct 2013 11:46:22 -0500 Subject: Re: [Histonet] Undecalcified sample in paraffin and plastic media From: ratliffjack@gmail.com To: ruio7@hotmail.com Rui, Did you need any additional assistance? Please let me know if there is anything I can do to be of assistance to you. Best Regards, Jack Jack L Ratliff, Owner/HistologistRatliff Histology Consultants, LLC389 Nichol Mill LaneFranklin, TN 37067 (615) 236-4901 (o)(615) 236-4962 (f)(317) 281-1975 (c) ratliffjack@gmail.comjratliff@ratliffhistology.com jratliff@ratliffhistology.com (coming soon) On Mon, Sep 30, 2013 at 7:49 AM, Jack Ratliff wrote: Rui, You will definitely want to consider using plastic media like methyl methacrylate (MMA). It will cause less shrinkage in the tissue during polymerization, you can still cut at a range of 4-12 microns using a rotary microtome and tungsten-carbide knife, any mineralization present in the tissue will infiltrate and polymerize well allowing for enhanced stabilization of tissue and section morphology throughout microtomy, and you can even deplastify the sections with certain MMA formulations to increase staining options. Please let me know if you do wish to continue with plastic media as I have helped many labs to get started with and/or to refine their current capabilities with MMA. Additionally, I would like to point out that I Chair the Hard Tissue Committee (HTC) for the National Society for Histotechnology (NSH). Membership with the NSH has several benefits that could also help you to move forward with your project at your own pace. For example, as a member you will have access to all archived publications of the Journal of Histotechnology (JOH). With this access to the JOH via Manny Publishing, the HTC has created a reference document that collates all relevant publications (1970's to present) that pertain to bone, biomaterials, medical device implants, resin histology, etc., so that one can easily locate and obtain publication information relevant to their niche specific needs. Rest assured that I will be happy to help you either way you choose to move forward. Best Regards, Jack On Sep 23, 2013, at 9:19 PM, Rui TAHARA wrote: > > > I have undecalcified paraffin embed samples > that were sectioned at 10 micron that I want to stain with Von kossa. Because > samples are embryonic quail heads (ossification starts to happen) and still > soft enough to section with standard rotary microtome with tungsten knife in paraffin. > > > My intention is to 3D reconstruct anatomies > based on histological sections. Because of this, I am wondering if I should actually > use plastic media rather than paraffin to keep the section shape as consistent > as possible. Does plastic embed material actually preserve the consistent shape > among sections better than paraffin embed sample? No winkle etc..? Is there any > other advantage that I actually should use the plastic media than paraffin for what > I want to do? I know downside of plastic media is that in general plastic > embedding process are lengthy and plastic embedding material are expensive than > the paraffin ones, and are mainly use for bone to support the hard material for > sectioning. > > When I sectioned some ossified samples, beak > start to fall off from section and the section show the lines from the possibly > scratched knife. Is this indication of paraffin media that does not provide enough > strength for sectioning? I thought it may possibly the poor infiltration. > > > > In our lab nobody has processed the plastic > embedding and sectioning (we have only standard microtome, no vaccum machine. Can > I section plastic embed sample with the standard microtome at 10 micron?) so I would > like to have any input before actually making a plastic embed sample. Any > suggestions would be appreciated. > > > Rui TAHARA > Biology Department > McGill University > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From one_angel_secret <@t> yahoo.com Mon Oct 7 10:57:44 2013 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Mon Oct 7 10:57:59 2013 Subject: [Histonet] HT HistoDeck question... In-Reply-To: References: <9F3CFEE76E51B64991C7485270890B40497D12D6@EX5.lj.gnf.org> <8FAB0141C65B42098CE4F110ECE9F372@HP2010> <351A66CE7FB11D40AB8AC8FE5559EC7E11A975A411@ushpwbmsmmp008.one.ads.bms.com> <335B9C2DCE414D1CB831DCCB07DEDC94@HP2010> Message-ID: <4720921C-FC87-4853-83E8-DEF8270466F7@yahoo.com> I think the problem with "a" is that 37% formaldehyde is what is considered 100% formalin not 10% nbf. So for all technical purposes I've never known of anyone fixing samples in 100% formalin either But I would agree it depends on what your doing with the sample if it got acetone or not. So i agree with you if they are asking what the fixative is for the fs to go for perm then they need to clarify and fix what they are saying for option "a". Im not sure but it seems like they are trying to pass 37% formaldehyde off as 10% formalin. Anyone else get that feeling? Sent from my iPhone On Oct 3, 2013, at 10:39 AM, "McKenzie, Emily" wrote: > Honestly I think this all depends on what they are actually asking. Are they asking what you fix the tissue in after the frozen is complete and you need to submit the remaining tissue for routine processing? If so, then A is the correct answer. > Are they asking what you fix the tissue to the slide with to stain the frozen section for immediate diagnosis? If so, then the answer can be several possibilities depending on what you are going to be staining/ the process you will be staining with. > I have worked in several different hospitals and each have a different protocol for there frozen section staining. In order to fix the tissue to the slide I have used 95% alcohol and acidic acid alcohol. The only time I have used formaldehyde is when I use the vapors for fat staining. > I think this might be a question to take up with ASCP. They are the ones administering the test. > > Emily K. McKenzie BS, HT(ASCP) > > Memorial Medical Center?701 North First Street?Springfield, IL 62781 > Ph: 217-788-3991?email: McKenzie.Emily@mhsil.com > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk > Sent: Thursday, October 03, 2013 9:22 AM > To: Watson, Linda; Stephenson, Sheryl; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] HT HistoDeck question... > > I agree, there is probably more than one correct answer to this question, depending upon whether you are planning on doing stains for lipids, IHC, immunofluoresence or muscle enzymes. > > But I don't think (A) full strength 37-40% formaldehyde solution would ever be the correct answer. Unless you put a gauze in the bottom on the coplin jar, pour a little conc. formaldehyde on the gauze, put a lid on the coplin jar, and fix the section in formaldehyde vapors. But the question does say SOLUTION, not VAPOR. So I still think A is wrong. And most likely full strength acetic acid (C) is wrong - would eat the tissue off the slide. > > That leaves cold acetone (B) , which is good for some antibodies and some enzymes, or alcoholic formalin (D) which might be OK, but most of the time things either like alcohol and hate formalin, or they like formalin and don't like alcohol. So I would think most FS that we want to fix would not particularly like alcoholic formalin. > > And none of the solutions listed are good for lipids. > > So, given the question (with incomplete information) and the choices of answers, I would still side with (B) cold acetone. > > Now - a little aside - for the questions on the ASCP HT and HTL exams - if it is a new question, the people on the HT/HTL exam committee would be looking at it intensely before it goes on the exam for the first time. If the committee people are having problems answering it (like we are here), the question would be reworked until all the issues are resolved (such as putting in "for lymph node IHC" into the question). If it makes it past the committee, and the stats from the exam show that many people are having problems answering it, the question is pulled from the exam and is not used in the person's score. The question is then sent back to the HT/HTL exam committee, to try to figure out why examinees were having problems, and the question reworked again. > > As someone who has written exam questions at the school for 20+ years, I can tell you that it really is hard to write exam questions. I think I've covered everything in the question so that it is straight forward, and then half the students read something into the question that I never thought of, or come up with a written answer that I never considered. So I either have to throw out the question or give the point to the student, depending upon what's going on. And then mark the question for a re-write next year. And that doesn't include me marking the wrong answer on my master sheet. It happens! > > Peggy A. Wenk, HTL(ASCP)SLS > > -----Original Message----- > From: Watson, Linda > Sent: Thursday, October 03, 2013 9:40 AM > To: Lee & Peggy Wenk ; Stephenson, Sheryl ; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] HT HistoDeck question... > > For frozen cut sections, would the fixation also depend on what you plan on doing with it. For example, H&E, Special Stain or IHC? Please correct if I am wrong. I think that is a trick question!!! > >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet- >> bounces@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk >> Sent: Thursday, October 03, 2013 8:16 AM >> To: Stephenson, Sheryl; histonet@lists.utsouthwestern.edu >> Subject: Re: [Histonet] HT HistoDeck question... >> >> Personally, I think it's "a" is a wrong answer, and that you are >> correct that "b" is a better answer. My students and I have found a >> couple of other questions that we thought had the wrong answer >> indicated in the study set. >> >> Peggy A. Wenk, HTL(ASCP)SLS >> -----Original Message----- >> From: Stephenson, Sheryl >> Sent: Thursday, October 03, 2013 7:21 AM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] HT HistoDeck question... >> >> Hi, >> Please clarify why this answer to the HistoDeck study question is a) >> and not b). >> >> Here is the question: >> >> 'Frozen section slides cut from fresh, unfixed tissue specimens are >> optimally fixed in which of the following solutions? >> a) 37%-40% formaldehyde >> b) Cold acetone >> c) Acetic acid alcohol >> d) Alcoholic formalin >> >> Thanks, >> >> >> Sheryl Stephenson | Histology Technician >> >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brannon <@t> alliedsearchpartners.com Mon Oct 7 12:54:48 2013 From: brannon <@t> alliedsearchpartners.com (Brannon Owens) Date: Mon Oct 7 12:54:59 2013 Subject: [Histonet] Histology Openings in New York Message-ID: We are currently recruiting for positions on multiple shifts for a client in New York. New York lab license is required to be considered. If interested please send me an updated resume, and as always a complete list of openings can be found on our website. 1) Grossing Technician- 3rd shift 2) Grossing Technician (per diem)- 3rd shift 3) Histotechnician/Histotechnologist- 1st shift 4) Histotechnician/Histotechnologist (IHC)- 2nd shift To view a complete list of Allied Search Partners current openings go to: http://www.alliedsearchpartners.com/careers.php -- Brannon Owens Recruitment Manager Allied Search Partners LinkedIn: http://www.linkedin.com/pub/brannon-owens/28/528/823 http://www.alliedsearchpartners.com T: 888.388.7571 ext. 106 F: 888.388.7572 From loriedaniels124 <@t> hotmail.com Mon Oct 7 20:21:47 2013 From: loriedaniels124 <@t> hotmail.com (lorie daniels) Date: Mon Oct 7 20:21:52 2013 Subject: [Histonet] (no subject) Message-ID: I would like to know if anyone out there has dealt with the new lica overnight processor. now when a person is going to do a 2 day delay, now my understanding is that if you do not press start this machine will not fill with formalin? but yet I was told it does? can someone please answer this for me Sent from Windows Mail From kim.p.kowsz <@t> pfizer.com Mon Oct 7 20:28:44 2013 From: kim.p.kowsz <@t> pfizer.com (Kowsz, Kim P) Date: Mon Oct 7 20:28:49 2013 Subject: [Histonet] histonet posting Message-ID: <33396311FD13C145AC7F059E86E8E5E650888189@NDHAMREXDE06.amer.pfizer.com> A brain artifact (vacuoles in the white matter) was noted in rat brain after extended fixation. An experiment showed that rat brains fixed in 10% neutral buffered formalin for 4, 10, 15, 20, or 25 days showed a progression of this artifact starting minimally at day 10 and getting progressively more prominent through day 25. The tissue processing schedule was standard throughout. The brains stayed in the same formalin through the fixation period. Have others experienced this artifact. I'm familiar with alcohol causing a similar artifact but have others observed a vacuole artifact in the white matter of brain with formalin fixation? Has my fixative deteriorated, if so, how and what is the chemical effect? Kim Kowsz Kim.p.kowsz@pfizer.com Pfizer Internal Use From shehnazster <@t> gmail.com Tue Oct 8 07:16:29 2013 From: shehnazster <@t> gmail.com (shehnaz khan) Date: Tue Oct 8 07:16:34 2013 Subject: [Histonet] analytical specificity and interferring substances Message-ID: Hello Histonetters, Could someone kindly shed some light on the follwing CAP standard and how to implement for histology and cytology: *Analytical Specificity / Interferring Substances:* For modified FDA-cleared / approved tests or laboratory developed tests, the results of each validation study include a sufficient number of samples to establish the test's analytical specificity. NOTE: The analytic specificity refers to the ability of a test or procedure to correctly identify or quantify an entity in the presence of interferring or cross-reactive substances that might be expected to be present. A million thanks S. Kahn From joelleweaver <@t> hotmail.com Tue Oct 8 07:59:24 2013 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Tue Oct 8 07:59:29 2013 Subject: [Histonet] analytical specificity and interferring substances In-Reply-To: References: Message-ID: In dealing with this in SOPs I referred to the calculation methods outlined in the CLSI guidelines for technical procedures such the ones published for IHC/ISH & FISH , which helped me since most of the probes are at least ASR and I will potentially work with LDT's ( particularly with probes) . By my recollection the discussions in these guidelines provides examples of studies and formulas for calculation and determination of analytic sensitivity and specificity ( sorry don't have handy at the moment to provide a citation or page# and put this together more than 6 months ago), but I got my copies of the guidelines from the NSH site, and they are easy to find. My approach was to base the minimum case/slide requirements for pilot and trials for LDT's to more strict parameters than my ASR methods, and I just made worksheets to record results and for sign off similar to other types of validation, just more detailed. The most challenging part is quantifying/establishing measurements & values. For any IHC evaluation this applies to, I used my usual qualitative scoring criteria (with an assigned quality score # for IHC) and carried this across IVD/ASR/LDT for consistency. When there is an ASCO or other score method, I incorporated those criteria. The probes are easier to define since the scoring is inherently more quantitative. I used the scoring criteria from the specification sheet for the most part for those. For doing the S & S trials, I had the initial calculations and documentation performed by the validating technologist, with review and final determination and calculation of analytic S & S- as well as the clinical application & utility to be made by the Medical Director. MD handles the report language. This was just my approach....I welcome critique and positive suggestions from the others. Joelle Weaver MAOM, HTL (ASCP) QIHC > Date: Tue, 8 Oct 2013 15:16:29 +0300 > From: shehnazster@gmail.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] analytical specificity and interferring substances > > Hello Histonetters, > > Could someone kindly shed some light on the follwing CAP standard and how > to implement for histology and cytology: > > *Analytical Specificity / Interferring Substances:* > For modified FDA-cleared / approved tests or laboratory developed tests, > the results of each validation study include a sufficient number of samples > to establish the test's analytical specificity. > NOTE: The analytic specificity refers to the ability of a test or procedure > to correctly identify or quantify an entity in the presence of interferring > or cross-reactive substances that might be expected to be present. > > A million thanks > > S. Kahn > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Andrew.Lisowski <@t> LeicaBiosystems.com Tue Oct 8 08:06:10 2013 From: Andrew.Lisowski <@t> LeicaBiosystems.com (Andrew.Lisowski@LeicaBiosystems.com) Date: Tue Oct 8 08:06:13 2013 Subject: [Histonet] test Message-ID: test (Embedded image moved to file: pic10712.jpg) Andrew R. Lisowski, M.S., HTL (A.S.C.P.) Technical Applications Manager, R&D Leica Biosystems, Inc. 5205 Route 12 Richmond, IL 60071 Office: 815-678-2000 x 6141 Mobile: 815-687-6186 Fax: 815-678-6044 Andrew.Lisowski@LeicaBiosystems.com _____________________________________________________________________ This e-mail has been scanned for viruses by Verizon Business Internet Managed Scanning Services - powered by MessageLabs. For further information visit http://www.verizonbusiness.com/uk From Andrew.Lisowski <@t> LeicaBiosystems.com Tue Oct 8 08:40:23 2013 From: Andrew.Lisowski <@t> LeicaBiosystems.com (Andrew.Lisowski@LeicaBiosystems.com) Date: Tue Oct 8 08:40:45 2013 Subject: [Histonet] Phantom rapid processor question Message-ID: Hello All, I am trying to learn more about this new processor where the manufacturer claims "Phantom Paraffin is designed to last up to two years in our paraffin bath". It also is a "single reagent processing", whatever it means.. Does anyone have this unit or knows how it uses its single reagent? is it IPA? is paraffin being heated to evaporate solvent carry-overs? I contacted the manufacturer but they never got back to me. thanks, Andrew (Embedded image moved to file: pic02600.jpg) Andrew R. Lisowski, M.S., HTL (A.S.C.P.) Technical Applications Manager, R&D Leica Biosystems, Inc. 5205 Route 12 Richmond, IL 60071 Office: 815-678-2000 x 6141 Mobile: 815-687-6186 Fax: 815-678-6044 Andrew.Lisowski@LeicaBiosystems.com _____________________________________________________________________ This e-mail has been scanned for viruses by Verizon Business Internet Managed Scanning Services - powered by MessageLabs. For further information visit http://www.verizonbusiness.com/uk From sdysart <@t> mirnarx.com Tue Oct 8 08:46:29 2013 From: sdysart <@t> mirnarx.com (Sarah Dysart) Date: Tue Oct 8 08:46:36 2013 Subject: [Histonet] In situ PCR Message-ID: <5996c8c5cc0d4c208db4c30309919658@BY2PR07MB106.namprd07.prod.outlook.com> Anyone out there do this? If so, during the PCR step you are amplifying your gene of interest, where does the amplified product go? Each step of the PCR (from how I am understanding this...I'm new to molecular biology protocols...) separates the double stranded sequence then copies it, and this goes on and on for 30-40 cycles...where does the product go? Does it just wash off? If not how is it binding to the tissue? Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 From Nancy_Schmitt <@t> pa-ucl.com Tue Oct 8 12:17:29 2013 From: Nancy_Schmitt <@t> pa-ucl.com (Nancy Schmitt) Date: Tue Oct 8 12:17:35 2013 Subject: [Histonet] FW: Dress codes Message-ID: <906B4DA90ED1DB4DB6C7E94D7CEE6C368142F56B@PEITHA.wad.pa-ucl.com> Not sure if my mail is going through or not........... Thank you Nancy Schmitt Nancy Schmitt HT, MLT(ASCP) Histology Coordinator United Clinical Laboratories 205 Bluff Street Dubuque, IA 52001 563-556-2010 ext. 142 nancy_schmitt@pa-ucl.com From: Nancy Schmitt Sent: Tuesday, October 08, 2013 8:58 AM To: 'histonet@lists.utsouthwestern.edu' Subject: Dress codes Good Morning- I am looking for input on dress codes - specifically shoe and pants guidelines and what regulatory agency your information comes from. It seems this can be a hot button topic:( Thank you for your input- Nancy Nancy Schmitt HT, MLT(ASCP) Histology Coordinator United Clinical Laboratories nancy_schmitt@pa-ucl.com NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From HornHV <@t> archildrens.org Tue Oct 8 12:22:57 2013 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Tue Oct 8 12:23:02 2013 Subject: [Histonet] RE: Dress codes In-Reply-To: <906B4DA90ED1DB4DB6C7E94D7CEE6C368142F56B@PEITHA.wad.pa-ucl.com> References: <906B4DA90ED1DB4DB6C7E94D7CEE6C368142F56B@PEITHA.wad.pa-ucl.com> Message-ID: <25A4DE08332B19499904459F00AAACB719C704FE31@EVS1.archildrens.org> We have a hospital dress code. It is not just for the lab. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt Sent: Tuesday, October 08, 2013 12:17 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FW: Dress codes Not sure if my mail is going through or not........... Thank you Nancy Schmitt Nancy Schmitt HT, MLT(ASCP) Histology Coordinator United Clinical Laboratories 205 Bluff Street Dubuque, IA 52001 563-556-2010 ext. 142 nancy_schmitt@pa-ucl.com From: Nancy Schmitt Sent: Tuesday, October 08, 2013 8:58 AM To: 'histonet@lists.utsouthwestern.edu' Subject: Dress codes Good Morning- I am looking for input on dress codes - specifically shoe and pants guidelines and what regulatory agency your information comes from. It seems this can be a hot button topic:( Thank you for your input- Nancy Nancy Schmitt HT, MLT(ASCP) Histology Coordinator United Clinical Laboratories nancy_schmitt@pa-ucl.com NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From Terra.Wineman <@t> novusint.com Tue Oct 8 12:34:49 2013 From: Terra.Wineman <@t> novusint.com (Wineman, Terra) Date: Tue Oct 8 12:34:56 2013 Subject: [Histonet] RE: Dress codes In-Reply-To: <25A4DE08332B19499904459F00AAACB719C704FE31@EVS1.archildrens.org> References: <906B4DA90ED1DB4DB6C7E94D7CEE6C368142F56B@PEITHA.wad.pa-ucl.com> <25A4DE08332B19499904459F00AAACB719C704FE31@EVS1.archildrens.org> Message-ID: <1EB8F245A303564EADF12AC7022FA74D70F1A7E5@NOVUS-EX01.novusint.com> In the research lab the dress code is an follows, close toed shoes this means no crocs or dress shoes with long pants, no shorts or dresses. Safety glasses per OSHA and nothing loose that might get caught in machinery. Terra Wineman, HTL (ASCP)CM Research Biologist 636-926-7476 phone terra.wineman@novusint.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Tuesday, October 08, 2013 12:23 PM To: 'Nancy Schmitt'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Dress codes We have a hospital dress code. It is not just for the lab. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt Sent: Tuesday, October 08, 2013 12:17 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FW: Dress codes Not sure if my mail is going through or not........... Thank you Nancy Schmitt Nancy Schmitt HT, MLT(ASCP) Histology Coordinator United Clinical Laboratories 205 Bluff Street Dubuque, IA 52001 563-556-2010 ext. 142 nancy_schmitt@pa-ucl.com From: Nancy Schmitt Sent: Tuesday, October 08, 2013 8:58 AM To: 'histonet@lists.utsouthwestern.edu' Subject: Dress codes Good Morning- I am looking for input on dress codes - specifically shoe and pants guidelines and what regulatory agency your information comes from. It seems this can be a hot button topic:( Thank you for your input- Nancy Nancy Schmitt HT, MLT(ASCP) Histology Coordinator United Clinical Laboratories nancy_schmitt@pa-ucl.com NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Terra.Wineman <@t> novusint.com Tue Oct 8 12:38:35 2013 From: Terra.Wineman <@t> novusint.com (Wineman, Terra) Date: Tue Oct 8 12:38:41 2013 Subject: [Histonet] RE: Dress codes References: <906B4DA90ED1DB4DB6C7E94D7CEE6C368142F56B@PEITHA.wad.pa-ucl.com> <25A4DE08332B19499904459F00AAACB719C704FE31@EVS1.archildrens.org> Message-ID: <1EB8F245A303564EADF12AC7022FA74D70F1A7F3@NOVUS-EX01.novusint.com> Regulatory agencies - OSHA, NIH, CDC for sure!! Terra Wineman, HTL (ASCP)CM Research Biologist 636-926-7476 phone terra.wineman@novusint.com -----Original Message----- From: Wineman, Terra Sent: Tuesday, October 08, 2013 12:35 PM To: 'Horn, Hazel V'; 'Nancy Schmitt'; histonet@lists.utsouthwestern.edu Subject: RE: Dress codes In the research lab the dress code is an follows, close toed shoes this means no crocs or dress shoes with long pants, no shorts or dresses. Safety glasses per OSHA and nothing loose that might get caught in machinery. Terra Wineman, HTL (ASCP)CM Research Biologist 636-926-7476 phone terra.wineman@novusint.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Tuesday, October 08, 2013 12:23 PM To: 'Nancy Schmitt'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Dress codes We have a hospital dress code. It is not just for the lab. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt Sent: Tuesday, October 08, 2013 12:17 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FW: Dress codes Not sure if my mail is going through or not........... Thank you Nancy Schmitt Nancy Schmitt HT, MLT(ASCP) Histology Coordinator United Clinical Laboratories 205 Bluff Street Dubuque, IA 52001 563-556-2010 ext. 142 nancy_schmitt@pa-ucl.com From: Nancy Schmitt Sent: Tuesday, October 08, 2013 8:58 AM To: 'histonet@lists.utsouthwestern.edu' Subject: Dress codes Good Morning- I am looking for input on dress codes - specifically shoe and pants guidelines and what regulatory agency your information comes from. It seems this can be a hot button topic:( Thank you for your input- Nancy Nancy Schmitt HT, MLT(ASCP) Histology Coordinator United Clinical Laboratories nancy_schmitt@pa-ucl.com NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TMcNemar <@t> lmhealth.org Tue Oct 8 12:49:56 2013 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Tue Oct 8 12:50:04 2013 Subject: [Histonet] RE: Dress codes In-Reply-To: <1EB8F245A303564EADF12AC7022FA74D70F1A7F3@NOVUS-EX01.novusint.com> References: <906B4DA90ED1DB4DB6C7E94D7CEE6C368142F56B@PEITHA.wad.pa-ucl.com> <25A4DE08332B19499904459F00AAACB719C704FE31@EVS1.archildrens.org> <1EB8F245A303564EADF12AC7022FA74D70F1A7F3@NOVUS-EX01.novusint.com> Message-ID: Hospital dress code. Scrub uniforms purchased through the hospital. No open toed shoes. None-uniformed employees are casual/business attire. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Wineman, Terra Sent: Tuesday, October 08, 2013 1:39 PM To: Wineman, Terra; Horn, Hazel V; 'Nancy Schmitt'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Dress codes Regulatory agencies - OSHA, NIH, CDC for sure!! Terra Wineman, HTL (ASCP)CM Research Biologist 636-926-7476 phone terra.wineman@novusint.com -----Original Message----- From: Wineman, Terra Sent: Tuesday, October 08, 2013 12:35 PM To: 'Horn, Hazel V'; 'Nancy Schmitt'; histonet@lists.utsouthwestern.edu Subject: RE: Dress codes In the research lab the dress code is an follows, close toed shoes this means no crocs or dress shoes with long pants, no shorts or dresses. Safety glasses per OSHA and nothing loose that might get caught in machinery. Terra Wineman, HTL (ASCP)CM Research Biologist 636-926-7476 phone terra.wineman@novusint.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Tuesday, October 08, 2013 12:23 PM To: 'Nancy Schmitt'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Dress codes We have a hospital dress code. It is not just for the lab. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt Sent: Tuesday, October 08, 2013 12:17 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FW: Dress codes Not sure if my mail is going through or not........... Thank you Nancy Schmitt Nancy Schmitt HT, MLT(ASCP) Histology Coordinator United Clinical Laboratories 205 Bluff Street Dubuque, IA 52001 563-556-2010 ext. 142 nancy_schmitt@pa-ucl.com From: Nancy Schmitt Sent: Tuesday, October 08, 2013 8:58 AM To: 'histonet@lists.utsouthwestern.edu' Subject: Dress codes Good Morning- I am looking for input on dress codes - specifically shoe and pants guidelines and what regulatory agency your information comes from. It seems this can be a hot button topic:( Thank you for your input- Nancy Nancy Schmitt HT, MLT(ASCP) Histology Coordinator United Clinical Laboratories nancy_schmitt@pa-ucl.com NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From PAMarcum <@t> uams.edu Tue Oct 8 13:00:50 2013 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Tue Oct 8 13:00:56 2013 Subject: [Histonet] RE: Dress codes In-Reply-To: References: <906B4DA90ED1DB4DB6C7E94D7CEE6C368142F56B@PEITHA.wad.pa-ucl.com> <25A4DE08332B19499904459F00AAACB719C704FE31@EVS1.archildrens.org> <1EB8F245A303564EADF12AC7022FA74D70F1A7F3@NOVUS-EX01.novusint.com> Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA32D315C5F6@Mail2Node2.ad.uams.edu> The hospital code for the laboratory is long pants, no open toed shoes, appropriate scrub or other tops. The hospital does not provide the scrubs here. We can buy what we want as long as they are not over the top. Most hospitals have similar codes that are linked to safety and professional appearance. Those who chose not to wear scrubs must still wear long pants, no open toed shoes and appropriate tops. If you are working in the lab and do not have scrubs on a lab coat is necessary. As it was explained to me long ago, the long pants and closed shoes protect you from spills of reagents or fluids that could cause health issues or even burns in the case of acids. The lab coats do the same and protect your clothing. Pam Marcum -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Tuesday, October 08, 2013 12:50 PM To: 'Wineman, Terra'; Horn, Hazel V; 'Nancy Schmitt'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Dress codes Hospital dress code. Scrub uniforms purchased through the hospital. No open toed shoes. None-uniformed employees are casual/business attire. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Wineman, Terra Sent: Tuesday, October 08, 2013 1:39 PM To: Wineman, Terra; Horn, Hazel V; 'Nancy Schmitt'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Dress codes Regulatory agencies - OSHA, NIH, CDC for sure!! Terra Wineman, HTL (ASCP)CM Research Biologist 636-926-7476 phone terra.wineman@novusint.com -----Original Message----- From: Wineman, Terra Sent: Tuesday, October 08, 2013 12:35 PM To: 'Horn, Hazel V'; 'Nancy Schmitt'; histonet@lists.utsouthwestern.edu Subject: RE: Dress codes In the research lab the dress code is an follows, close toed shoes this means no crocs or dress shoes with long pants, no shorts or dresses. Safety glasses per OSHA and nothing loose that might get caught in machinery. Terra Wineman, HTL (ASCP)CM Research Biologist 636-926-7476 phone terra.wineman@novusint.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Tuesday, October 08, 2013 12:23 PM To: 'Nancy Schmitt'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Dress codes We have a hospital dress code. It is not just for the lab. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt Sent: Tuesday, October 08, 2013 12:17 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FW: Dress codes Not sure if my mail is going through or not........... Thank you Nancy Schmitt Nancy Schmitt HT, MLT(ASCP) Histology Coordinator United Clinical Laboratories 205 Bluff Street Dubuque, IA 52001 563-556-2010 ext. 142 nancy_schmitt@pa-ucl.com From: Nancy Schmitt Sent: Tuesday, October 08, 2013 8:58 AM To: 'histonet@lists.utsouthwestern.edu' Subject: Dress codes Good Morning- I am looking for input on dress codes - specifically shoe and pants guidelines and what regulatory agency your information comes from. It seems this can be a hot button topic:( Thank you for your input- Nancy Nancy Schmitt HT, MLT(ASCP) Histology Coordinator United Clinical Laboratories nancy_schmitt@pa-ucl.com NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From algranth <@t> email.arizona.edu Tue Oct 8 13:43:50 2013 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Tue Oct 8 13:43:55 2013 Subject: [Histonet] RE: Dress codes In-Reply-To: <41D3A1AF6FEF0643BDC89E0516A6EA32D315C5F6@Mail2Node2.ad.uams.edu> References: <906B4DA90ED1DB4DB6C7E94D7CEE6C368142F56B@PEITHA.wad.pa-ucl.com> <25A4DE08332B19499904459F00AAACB719C704FE31@EVS1.archildrens.org> <1EB8F245A303564EADF12AC7022FA74D70F1A7F3@NOVUS-EX01.novusint.com> <41D3A1AF6FEF0643BDC89E0516A6EA32D315C5F6@Mail2Node2.ad.uams.edu> Message-ID: I'm sure that there is a dress code here but I don't think many adhere to it. Since I started to work here 15 yrs ago I've worn nothing but jeans, shorts, sandals (OMG!) like flip flops, and T-shirts. I have appropriate attire in a drawer just in case. I know you all might think this is awful, unsafe and whatever so fire away. Don't care. Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 From doug.porter <@t> caplab.org Tue Oct 8 14:23:41 2013 From: doug.porter <@t> caplab.org (Douglas Porter) Date: Tue Oct 8 14:21:40 2013 Subject: [Histonet] RE: Dress codes In-Reply-To: References: <906B4DA90ED1DB4DB6C7E94D7CEE6C368142F56B@PEITHA.wad.pa-ucl.com> <25A4DE08332B19499904459F00AAACB719C704FE31@EVS1.archildrens.org> <1EB8F245A303564EADF12AC7022FA74D70F1A7F3@NOVUS-EX01.novusint.com> Message-ID: <005001cec45b$e68ea860$b3abf920$@caplab.org> Some go so far as to say the shoes must not have ventilation in the toe area where spills can pass through. This requires that the shoes be suede, leather or some other non-ventilated somewhat repellant kind. No running shoes for example. Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.porter@caplab.org www.caplab.org ? ? The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, copying, forwarding or capture of this communication is strictly prohibited. If you have received this communication in error, please notify me immediately by return e-mail and delete this and all copies. Thank-you. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Tuesday, October 08, 2013 1:50 PM To: 'Wineman, Terra'; Horn, Hazel V; 'Nancy Schmitt'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Dress codes Hospital dress code. Scrub uniforms purchased through the hospital. No open toed shoes. None-uniformed employees are casual/business attire. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Wineman, Terra Sent: Tuesday, October 08, 2013 1:39 PM To: Wineman, Terra; Horn, Hazel V; 'Nancy Schmitt'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Dress codes Regulatory agencies - OSHA, NIH, CDC for sure!! Terra Wineman, HTL (ASCP)CM Research Biologist 636-926-7476 phone terra.wineman@novusint.com -----Original Message----- From: Wineman, Terra Sent: Tuesday, October 08, 2013 12:35 PM To: 'Horn, Hazel V'; 'Nancy Schmitt'; histonet@lists.utsouthwestern.edu Subject: RE: Dress codes In the research lab the dress code is an follows, close toed shoes this means no crocs or dress shoes with long pants, no shorts or dresses. Safety glasses per OSHA and nothing loose that might get caught in machinery. Terra Wineman, HTL (ASCP)CM Research Biologist 636-926-7476 phone terra.wineman@novusint.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Tuesday, October 08, 2013 12:23 PM To: 'Nancy Schmitt'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Dress codes We have a hospital dress code. It is not just for the lab. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt Sent: Tuesday, October 08, 2013 12:17 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FW: Dress codes Not sure if my mail is going through or not........... Thank you Nancy Schmitt Nancy Schmitt HT, MLT(ASCP) Histology Coordinator United Clinical Laboratories 205 Bluff Street Dubuque, IA 52001 563-556-2010 ext. 142 nancy_schmitt@pa-ucl.com From: Nancy Schmitt Sent: Tuesday, October 08, 2013 8:58 AM To: 'histonet@lists.utsouthwestern.edu' Subject: Dress codes Good Morning- I am looking for input on dress codes - specifically shoe and pants guidelines and what regulatory agency your information comes from. It seems this can be a hot button topic:( Thank you for your input- Nancy Nancy Schmitt HT, MLT(ASCP) Histology Coordinator United Clinical Laboratories nancy_schmitt@pa-ucl.com NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** ********************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kim.tournear <@t> yahoo.com Tue Oct 8 16:04:16 2013 From: kim.tournear <@t> yahoo.com (Kim Tournear) Date: Tue Oct 8 16:04:20 2013 Subject: [Histonet] RE: Dress codes In-Reply-To: References: <906B4DA90ED1DB4DB6C7E94D7CEE6C368142F56B@PEITHA.wad.pa-ucl.com> <25A4DE08332B19499904459F00AAACB719C704FE31@EVS1.archildrens.org> <1EB8F245A303564EADF12AC7022FA74D70F1A7F3@NOVUS-EX01.novusint.com> <41D3A1AF6FEF0643BDC89E0516A6EA32D315C5F6@Mail2Node2.ad.uams.edu> Message-ID: Hey andi! I wanna work for u. LOL Sent from the iPhone of Kim Tournear ?? ? On Oct 8, 2013, at 11:43 AM, "Grantham, Andrea L - (algranth)" wrote: > I'm sure that there is a dress code here but I don't think many adhere to it. Since I started to work here 15 yrs ago I've worn nothing but jeans, shorts, sandals (OMG!) like flip flops, and T-shirts. I have appropriate attire in a drawer just in case. I know you all might think this is awful, unsafe and whatever so fire away. Don't care. > > > Andrea Grantham, HT (ASCP) > Senior Research Specialist > University of Arizona > Cellular and Molecular Medicine > Histology Service Laboratory > P.O.Box 245044 > Tucson, AZ 85724 > > algranth@email.arizona.edu > Tel: 520.626.4415 Fax: 520.626.2097 > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ewj <@t> pigsqq.org Tue Oct 8 18:34:43 2013 From: ewj <@t> pigsqq.org (=?UTF-8?B?IkUuIFdheW5lIEpvaG5zb24g5pyx56iz5qOuIg==?=) Date: Tue Oct 8 18:34:56 2013 Subject: [Histonet] In situ PCR In-Reply-To: <5996c8c5cc0d4c208db4c30309919658@BY2PR07MB106.namprd07.prod.outlook.com> References: <5996c8c5cc0d4c208db4c30309919658@BY2PR07MB106.namprd07.prod.outlook.com> Message-ID: <52549693.6040708@pigsqq.org> We don't do in-situ PCR, but the principle is that with formalin-fixed tissues your amplified product is trapped in the protein matrix on the slide. On 3:59, Sarah Dysart wrote: > Anyone out there do this? If so, during the PCR step you are amplifying your gene of interest, where does the amplified product go? Each step of the PCR (from how I am understanding this...I'm new to molecular biology protocols...) separates the double stranded sequence then copies it, and this goes on and on for 30-40 cycles...where does the product go? Does it just wash off? If not how is it binding to the tissue? > > Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) > Histotechnologist > Mirna Therapeutics > 2150 Woodward Street > Suite 100 > Austin, Texas 78744 > (512)901-0900 ext. 6912 > > > From hlukey <@t> msn.com Tue Oct 8 21:09:54 2013 From: hlukey <@t> msn.com (Hugh Luk) Date: Tue Oct 8 21:10:00 2013 Subject: [Histonet] Asbestos microtomy advice? In-Reply-To: References: Message-ID: Hi folks, Odd question (one I never expected until this week): if a researcher was injecting fairly large asbestos loads (~75-100 injections) into research mice, would you use extra precautions while performing microtomy? On thousands of tissue blocks? The injection sites are so numerous, we easily note the colors of the type of asbestos fiber-clusters while grossing. Our concern, is mainly in the act of trimming and microtomizing the tissue. Noteworthy publications for histology? Grateful for any advice (in advance), Hugh UH cancer center Hawaii From Jonathan.Cremer <@t> med.kuleuven.be Wed Oct 9 01:53:08 2013 From: Jonathan.Cremer <@t> med.kuleuven.be (Jonathan Cremer) Date: Wed Oct 9 01:53:21 2013 Subject: [Histonet] RE: Dress codes In-Reply-To: <005001cec45b$e68ea860$b3abf920$@caplab.org> References: <906B4DA90ED1DB4DB6C7E94D7CEE6C368142F56B@PEITHA.wad.pa-ucl.com> <25A4DE08332B19499904459F00AAACB719C704FE31@EVS1.archildrens.org> <1EB8F245A303564EADF12AC7022FA74D70F1A7F3@NOVUS-EX01.novusint.com> , <005001cec45b$e68ea860$b3abf920$@caplab.org> Message-ID: If you work with acids and bases (and other nasties) as in a synthetic chemistry lab, it's even a very good idea to wear cotton socks and trousers. Synthetics can melt into your skin when splashed with said chemicals, cotton will just be carbonized, giving you time to undress or run to an emergency shower. --- Jonathan Cremer Laboratory Technician TARGID - KU Leuven Gasthuisberg CDG; Labo Experimentele Immunologie; Herestraat 49 bus 811; 3000 Leuven; Belgium ________________________________________ Van: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] namens Douglas Porter [doug.porter@caplab.org] Verzonden: dinsdag 8 oktober 2013 21:23 Aan: 'Tom McNemar'; 'Wineman, Terra'; 'Horn, Hazel V'; 'Nancy Schmitt'; histonet@lists.utsouthwestern.edu Onderwerp: RE: [Histonet] RE: Dress codes Some go so far as to say the shoes must not have ventilation in the toe area where spills can pass through. This requires that the shoes be suede, leather or some other non-ventilated somewhat repellant kind. No running shoes for example. Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.porter@caplab.org www.caplab.org The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, copying, forwarding or capture of this communication is strictly prohibited. If you have received this communication in error, please notify me immediately by return e-mail and delete this and all copies. Thank-you. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Tuesday, October 08, 2013 1:50 PM To: 'Wineman, Terra'; Horn, Hazel V; 'Nancy Schmitt'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Dress codes Hospital dress code. Scrub uniforms purchased through the hospital. No open toed shoes. None-uniformed employees are casual/business attire. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Wineman, Terra Sent: Tuesday, October 08, 2013 1:39 PM To: Wineman, Terra; Horn, Hazel V; 'Nancy Schmitt'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Dress codes Regulatory agencies - OSHA, NIH, CDC for sure!! Terra Wineman, HTL (ASCP)CM Research Biologist 636-926-7476 phone terra.wineman@novusint.com -----Original Message----- From: Wineman, Terra Sent: Tuesday, October 08, 2013 12:35 PM To: 'Horn, Hazel V'; 'Nancy Schmitt'; histonet@lists.utsouthwestern.edu Subject: RE: Dress codes In the research lab the dress code is an follows, close toed shoes this means no crocs or dress shoes with long pants, no shorts or dresses. Safety glasses per OSHA and nothing loose that might get caught in machinery. Terra Wineman, HTL (ASCP)CM Research Biologist 636-926-7476 phone terra.wineman@novusint.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Tuesday, October 08, 2013 12:23 PM To: 'Nancy Schmitt'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Dress codes We have a hospital dress code. It is not just for the lab. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt Sent: Tuesday, October 08, 2013 12:17 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FW: Dress codes Not sure if my mail is going through or not........... Thank you Nancy Schmitt Nancy Schmitt HT, MLT(ASCP) Histology Coordinator United Clinical Laboratories 205 Bluff Street Dubuque, IA 52001 563-556-2010 ext. 142 nancy_schmitt@pa-ucl.com From: Nancy Schmitt Sent: Tuesday, October 08, 2013 8:58 AM To: 'histonet@lists.utsouthwestern.edu' Subject: Dress codes Good Morning- I am looking for input on dress codes - specifically shoe and pants guidelines and what regulatory agency your information comes from. It seems this can be a hot button topic:( Thank you for your input- Nancy Nancy Schmitt HT, MLT(ASCP) Histology Coordinator United Clinical Laboratories nancy_schmitt@pa-ucl.com NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** ********************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tahseen <@t> brain.net.pk Wed Oct 9 07:37:38 2013 From: tahseen <@t> brain.net.pk (tahseen) Date: Wed Oct 9 07:37:51 2013 Subject: [Histonet] P16 Message-ID: Hi All, I am looking P16 for HP V in Sq.ca of oral cavity, which company and clone will be good. I use Ab cam pathologist not comfortable. Thanks, Muhammad Tahseen Supervisor Histology SKMCH&RC Lahore Pakistan From rjbuesa <@t> yahoo.com Wed Oct 9 09:25:55 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Oct 9 09:26:01 2013 Subject: [Histonet] Asbestos microtomy advice? In-Reply-To: References: Message-ID: <1381328755.11132.YahooMailNeo@web163101.mail.bf1.yahoo.com> If there are so many sites injected and so much injected, during trimming you may expose some fibers and "maybe" they can float in the air (?), and maybe you can inhale them (?) and maybe you can get "asbestosis" (doubtful) BUT if you want to be "better safe than sorry", use a simple mouth/nose mask. (I personally think this would be an overkill!) Ren? J. ________________________________ From: Hugh Luk To: "histonet@lists.utsouthwestern.edu" Sent: Tuesday, October 8, 2013 10:09 PM Subject: [Histonet] Asbestos microtomy advice? Hi folks, Odd question (one I never expected until this week): if a researcher was injecting fairly large asbestos loads (~75-100 injections) into research mice, would you use extra precautions while performing microtomy?? On thousands of tissue blocks?? The injection sites are so numerous, we easily note the colors of the type of asbestos fiber-clusters while grossing.? Our concern, is mainly in the act of trimming and microtomizing the tissue.? Noteworthy publications for histology? Grateful for any advice (in advance), Hugh UH cancer center Hawaii ??? ??? ??? ? ??? ??? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From koellingr <@t> comcast.net Wed Oct 9 09:59:51 2013 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Wed Oct 9 10:00:10 2013 Subject: [Histonet] Asbestos microtomy advice? In-Reply-To: <1381328755.11132.YahooMailNeo@web163101.mail.bf1.yahoo.com> References: <1381328755.11132.YahooMailNeo@web163101.mail.bf1.yahoo.com> Message-ID: <1897448581.882491.1381330791774.JavaMail.root@comcast.net> Hugh, I agree with Rene about the possibilities and yes a mask might be overkill. But far, far, far more than that for me, the description of this "research" as stated bothers me greatly. I am surely no expert on this particular line of research but it is my understanding that projects looking at mesothelioma and such involve inhalation of asbestos or minute injections into flanks or pleural cavities. No matter the route of administration, if you are UH, your IACUC (Institutional Animal Care and Use Committee) is a federally mandated law governing experimentation on and treatment of animals. Along with several other groups with lettered names. When we or anyone I know does experimentation on animals, they have to absolutely define the limited amount and route of administration and it cannot be altered without prior approval. I could be very wrong, but I can't even imagine any committee following the rules allowing authorization of 75-100 apparently haphazard injections (IP? SQ? ID?) into a mouse much less do this apparently many, many mice. For what purpose? If I was cutting tissues, I wouldn't be so concerned about myself but I would sure want some answers to this exact procedure as you described. Ray Koelling Seattle, WA ----- Original Message ----- From: "Rene J Buesa" To: "Hugh Luk" , histonet@lists.utsouthwestern.edu Sent: Wednesday, October 9, 2013 7:25:55 AM Subject: Re: [Histonet] Asbestos microtomy advice? If there are so many sites injected and so much injected, during trimming you may expose some fibers and "maybe" they can float in the air (?), and maybe you can inhale them (?) and maybe you can get "asbestosis" (doubtful) BUT if you want to be "better safe than sorry", use a simple mouth/nose mask. (I personally think this would be an overkill!) Ren? J. ________________________________ From: Hugh Luk To: "histonet@lists.utsouthwestern.edu" Sent: Tuesday, October 8, 2013 10:09 PM Subject: [Histonet] Asbestos microtomy advice? Hi folks, Odd question (one I never expected until this week): if a researcher was injecting fairly large asbestos loads (~75-100 injections) into research mice, would you use extra precautions while performing microtomy? On thousands of tissue blocks? The injection sites are so numerous, we easily note the colors of the type of asbestos fiber-clusters while grossing. Our concern, is mainly in the act of trimming and microtomizing the tissue. Noteworthy publications for histology? Grateful for any advice (in advance), Hugh UH cancer center Hawaii _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MWeirauch <@t> crittenton.com Wed Oct 9 11:12:32 2013 From: MWeirauch <@t> crittenton.com (Maray Weirauch) Date: Wed Oct 9 11:12:22 2013 Subject: [Histonet] Pathology charges Message-ID: We've been told that before our pathologists can order/run any of these stains (CPT 88313, 88342 or 88360) on our pathology specimens, we must obtain a pre-authorization from the insurance carrier or the payment will be denied. Has anyone else run into this? How are you efficiently handling that? Maray Weirauch From JEllin <@t> yumaregional.org Wed Oct 9 11:20:26 2013 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Wed Oct 9 11:20:37 2013 Subject: [Histonet] RE: Pathology charges In-Reply-To: References: Message-ID: I did not know this was going on,, where is this being stated at -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Maray Weirauch Sent: Wednesday, October 09, 2013 9:13 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Pathology charges We've been told that before our pathologists can order/run any of these stains (CPT 88313, 88342 or 88360) on our pathology specimens, we must obtain a pre-authorization from the insurance carrier or the payment will be denied. Has anyone else run into this? How are you efficiently handling that? Maray Weirauch _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ From Richard.Cartun <@t> hhchealth.org Wed Oct 9 11:34:05 2013 From: Richard.Cartun <@t> hhchealth.org (Cartun, Richard) Date: Wed Oct 9 11:34:11 2013 Subject: [Histonet] Pancreatic trypsin IHC Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E01888522@HHCEXCHMB05.hhcsystem.org> Is anyone using a commercially-available anti-pancreatic trypsin antibody for diagnostic IHC? Thank you! Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax richard.cartun@hhchealth.org This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From Joyce.Weems <@t> emoryhealthcare.org Wed Oct 9 11:44:32 2013 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Wed Oct 9 11:44:49 2013 Subject: [Histonet] RE: Pathology charges In-Reply-To: References: Message-ID: I haven't heard of this. We do them on a daily basis and I have heard of no problems. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Maray Weirauch Sent: Wednesday, October 09, 2013 12:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Pathology charges We've been told that before our pathologists can order/run any of these stains (CPT 88313, 88342 or 88360) on our pathology specimens, we must obtain a pre-authorization from the insurance carrier or the payment will be denied. Has anyone else run into this? How are you efficiently handling that? Maray Weirauch _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From lblazek <@t> digestivespecialists.com Wed Oct 9 11:56:56 2013 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Wed Oct 9 11:57:02 2013 Subject: [Histonet] RE: Pathology charges In-Reply-To: References: Message-ID: <5A2BD13465E061429D6455C8D6B40E391666DE2CFE@IBMB7Exchange.digestivespecialists.com> I know you have to have the correct ICD-9 codes attached but have not heard of pre-authorization. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Maray Weirauch Sent: Wednesday, October 09, 2013 12:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Pathology charges We've been told that before our pathologists can order/run any of these stains (CPT 88313, 88342 or 88360) on our pathology specimens, we must obtain a pre-authorization from the insurance carrier or the payment will be denied. Has anyone else run into this? How are you efficiently handling that? Maray Weirauch _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From twheelock <@t> mclean.harvard.edu Wed Oct 9 15:34:37 2013 From: twheelock <@t> mclean.harvard.edu (Tim Wheelock) Date: Wed Oct 9 15:35:00 2013 Subject: [Histonet] Immunostaining after prolonged formalin immersion? Message-ID: <5255BDDD.3090800@mclean.harvard.edu> Hi Everyone: If tissue has been sitting in 10 % neutral buffered formalin for 3-5 years, can excellent immunostaining still be done on it. I am guessing that some sort of antigen retrieval technique can be done on the tissue, but I am not sure, since we don't do immunostaining ourselves. Thanks, Tim Tim Wheelock Harvard Brain Bank McLean Hospital Belmont, MA From Richard.Cartun <@t> hhchealth.org Wed Oct 9 15:51:48 2013 From: Richard.Cartun <@t> hhchealth.org (Cartun, Richard) Date: Wed Oct 9 15:51:56 2013 Subject: [Histonet] Immunostaining after prolonged formalin immersion? In-Reply-To: <5255BDDD.3090800@mclean.harvard.edu> References: <5255BDDD.3090800@mclean.harvard.edu> Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E018886D7@HHCEXCHMB05.hhcsystem.org> I have obtained excellent immunoreactivity for some proteins on various tissues that have been in formalin for more than a year; however, you won't know unless you try it and there are "internal positive controls" to validate negative results. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax richard.cartun@hhchealth.org ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Tim Wheelock [twheelock@mclean.harvard.edu] Sent: Wednesday, October 09, 2013 4:34 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Immunostaining after prolonged formalin immersion? Hi Everyone: If tissue has been sitting in 10 % neutral buffered formalin for 3-5 years, can excellent immunostaining still be done on it. I am guessing that some sort of antigen retrieval technique can be done on the tissue, but I am not sure, since we don't do immunostaining ourselves. Thanks, Tim Tim Wheelock Harvard Brain Bank McLean Hospital Belmont, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From Richard.Cartun <@t> hhchealth.org Wed Oct 9 15:58:38 2013 From: Richard.Cartun <@t> hhchealth.org (Cartun, Richard) Date: Wed Oct 9 15:58:56 2013 Subject: [Histonet] Negative Reagent Controls in Diagnostic IHC.... Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E018886EC@HHCEXCHMB05.hhcsystem.org> My colleagues and I presented a poster at the NSH annual meeting in Providence recently titled, "Negative Reagent Controls in Diagnostic Immunohistochemistry: Do we need them?". I have received a few requests for the actual poster (PowerPoint slide). I will be happy to e-mail it to anyone who is interested. Oh, by the way, we have determined that they are not needed in our laboratory and by eliminating them we have saved our laboratory over $100,000 a year! Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax richard.cartun@hhchealth.org This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From ggracie <@t> stvincents.com.au Wed Oct 9 17:42:12 2013 From: ggracie <@t> stvincents.com.au (Gary Gracie) Date: Wed Oct 9 17:42:23 2013 Subject: [Histonet] p16 Message-ID: Dear Muhammad, We are using MTM p16. This is now supplied by Roche cat# 06594441001. It is only sold as a pre-dilute but I dilute 1/2 and it still works very well. Regards Gary Gracie Senior Technical Officer IHC Laboratory Anatomical Pathology St Vincents Hospital Sydney Australia Hi All, I am looking P16 for HP V in Sq.ca of oral cavity, which company and clone will be good. I use Ab cam pathologist not comfortable. Thanks, Muhammad Tahseen Supervisor Histology SKMCH&RC Lahore Pakistan ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the system manager. This footnote also confirms that this email message has been virus scanned and although no viruses were detected by the system, St Vincents & Mater Health Sydney accepts no liability for any consequential damage resulting from email containing any computer viruses. ********************************************************************** From BSullivan <@t> virtua.org Thu Oct 10 07:33:45 2013 From: BSullivan <@t> virtua.org (Sullivan, Beatrice) Date: Thu Oct 10 07:33:51 2013 Subject: [Histonet] RE: Negative Reagent Controls in Diagnostic IHC.... In-Reply-To: <9215BD4B0BA1B44D962A71C758B68D2E018886EC@HHCEXCHMB05.hhcsystem.org> References: <9215BD4B0BA1B44D962A71C758B68D2E018886EC@HHCEXCHMB05.hhcsystem.org> Message-ID: <6932520047F7EE46B512E9801344F160380CBE04@ExchangeMB-1.Virtua.org> Okay so my question is.................how did you address the non- use if you happen to be required? (Joint Commission) Beatrice L. Sullivan Corporate Histology Supervisor Virtua Voorhees -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cartun, Richard Sent: Wednesday, October 09, 2013 4:59 PM To: Histonet Subject: [Histonet] Negative Reagent Controls in Diagnostic IHC.... My colleagues and I presented a poster at the NSH annual meeting in Providence recently titled, "Negative Reagent Controls in Diagnostic Immunohistochemistry: Do we need them?". I have received a few requests for the actual poster (PowerPoint slide). I will be happy to e-mail it to anyone who is interested. Oh, by the way, we have determined that they are not needed in our laboratory and by eliminating them we have saved our laboratory over $100,000 a year! Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax richard.cartun@hhchealth.org This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message, and any included attachments, are from Virtua Health or its related affiliates and is intended only for the addressee(s). The information contained herein is privileged, proprietary or may include confidential information and/or protected patient health information. Any unauthorized review, forwarding, printing, copying, distributing, or otherwise disseminating or taking any action based on such information is strictly prohibited. If you have received this message in error, or have reason to believe you are not authorized to receive it, please delete this message promptly and notify the sender by e-mail with a copy to ISSECURITY@virtua.org. Thank you From LSebree <@t> uwhealth.org Thu Oct 10 07:39:35 2013 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Thu Oct 10 07:39:40 2013 Subject: [Histonet] RE: Negative Reagent Controls in Diagnostic IHC.... In-Reply-To: <9215BD4B0BA1B44D962A71C758B68D2E018886EC@HHCEXCHMB05.hhcsystem.org> References: <9215BD4B0BA1B44D962A71C758B68D2E018886EC@HHCEXCHMB05.hhcsystem.org> Message-ID: <77DD817201982748BC67D7960F2F76AF06D050@UWHC-MBX12.uwhis.hosp.wisc.edu> Richard, I wish we could eliminate them but not only do our negatives displaying some non-specific staining, seems to be tissue dependent, but our in-house QA people say we need to continue using them per the data sheets accompanying many of our antibodies. We use Ventana UltraView DAB detection, a multimer kit. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cartun, Richard Sent: Wednesday, October 09, 2013 3:59 PM To: Histonet Subject: [Histonet] Negative Reagent Controls in Diagnostic IHC.... My colleagues and I presented a poster at the NSH annual meeting in Providence recently titled, "Negative Reagent Controls in Diagnostic Immunohistochemistry: Do we need them?". I have received a few requests for the actual poster (PowerPoint slide). I will be happy to e-mail it to anyone who is interested. Oh, by the way, we have determined that they are not needed in our laboratory and by eliminating them we have saved our laboratory over $100,000 a year! Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax richard.cartun@hhchealth.org This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ecrespo <@t> cmblabs.com Thu Oct 10 11:53:17 2013 From: ecrespo <@t> cmblabs.com (Ed Crespo) Date: Thu Oct 10 11:53:25 2013 Subject: [Histonet] India Ink for inking surgical margin borders Message-ID: <21511BF5-BA90-4392-A9B4-FF159441BC5A@cmblabs.com> I normally purchase India Ink from one of our vendors, but know it's also sold and used at artist supply shops. Does anyone know if I can used the artist india ink for Pathology use? Really, the only issue would be if the ink stays on the tissue during processing right? Please advise. Ed Crespo, CT(ASCP) Anatomic Pathology Manager Safety Officer 10700 Walker Street Cypress, CA 90630 phone: 714 880.3330 fax: 714 816.1511 email: ecrespo@cmblabs.com cmblabs.com The contents of this e-mail message, including any attachments, are intended solely for the use of the person or entity to whom this e-mail is addressed. It contains information that may be privileged, proprietary, confidential, and protected from disclosure by applicable state and federal law. Any Protected Health Information (PHI) contained in this email is HIGHLY CONFIDENTIAL. It is intended for the exclusive use of the addressee. It is to be used only to aid in providing specific healthcare services to the patient(s). Any other use is a violation of Federal Law (HIPAA) and will be reported as such. If you are not the intended recipient of this message, or the employee or agent responsible for delivering it to the intended recipient, you are hereby advised that reading, disseminating, distributing, use, or copying of the contents of this message is strictly prohibited. If you have received this message in error, or are not the named recipient(s), please notify the sender immediately by reply e-mail or by phone at (714) 657-7369 and delete this message and any attachments from your computer and any archival/backup copies. Thank you. From mucram11 <@t> comcast.net Thu Oct 10 12:04:29 2013 From: mucram11 <@t> comcast.net (Pam Marcum) Date: Thu Oct 10 12:04:35 2013 Subject: [Histonet] India Ink for inking surgical margin borders In-Reply-To: <21511BF5-BA90-4392-A9B4-FF159441BC5A@cmblabs.com> Message-ID: <1397924649.1381088.1381424669310.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> The only warning I know of and I have used India Ink is to be sure it is the "permanent" India Ink not the washable.? If you buy the non-permanent it will come off in processing.? ? Pam Marcum ----- Original Message ----- From: "Ed Crespo" To: histonet@lists.utsouthwestern.edu Sent: Thursday, October 10, 2013 11:53:17 AM Subject: [Histonet] India Ink for inking surgical margin borders I normally purchase India Ink from one of our vendors, but know it's also sold and used at artist supply shops. ?Does anyone know if I can used the artist india ink for Pathology use? ?Really, the only issue would be if the ink stays on the tissue during processing right? Please advise. Ed Crespo, CT(ASCP) Anatomic Pathology Manager Safety Officer 10700 Walker Street Cypress, CA 90630 phone: 714 880.3330 fax: 714 816.1511 email: ecrespo@cmblabs.com cmblabs.com ? ? The contents of this e-mail message, including any attachments, are intended solely for the use of the person or entity to whom this e-mail is addressed. ?It contains information that may be privileged, proprietary, confidential, and protected from disclosure by applicable state and federal law. ?Any Protected Health Information (PHI) contained in this email is HIGHLY CONFIDENTIAL. ?It is intended for the exclusive use of the addressee. ?It is to be used only to aid in providing specific healthcare services to the patient(s). ?Any other use is a violation of Federal Law (HIPAA) and will be reported as such. ?If you are not the intended recipient of this message, or the employee or agent responsible for delivering it to the intended recipient, you are hereby advised that reading, disseminating, distributing, use, or copying of the contents of this message is strictly prohibited. ?If you have received this message in error, or are not the named recipient(s), please notify the sender immediately by reply e-mail or by phone at (714) 657-7369 and delete this message and any attachments from your computer and any archival/backup copies. ?Thank you. ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Allison.Scott <@t> harrishealth.org Thu Oct 10 12:24:30 2013 From: Allison.Scott <@t> harrishealth.org (Scott, Allison D) Date: Thu Oct 10 12:24:33 2013 Subject: [Histonet] Novec 7100 Engineerd Fluid Message-ID: Hello to all in histoland. Is there anyone using the Novec 7100 fluid for freezing frozen blocks in a low temp freezing bath. We were getting it from fisher but they have discontinued it. I am trying to find another vendor. Any help will be appreciated. Allison Scott HT(ASCP) Supervisor, Histology Lab LBJ Hospital Harris Health System Office: 713-566-2148 Lab: 713-566-5287 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. From rsrichmond <@t> gmail.com Thu Oct 10 12:42:31 2013 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Thu Oct 10 12:42:35 2013 Subject: [Histonet] Re: India Ink for inking surgical margin borders Message-ID: Ed Crespo, CT(ASCP) in Cypress CA asks: >>I normally purchase India Ink from one of our vendors, but know it's also sold at artist supply shops. Does anyone know if I can use the artist india ink for Pathology? Really, the only issue would be if the ink stays on the tissue during processing right? Please advise.<< I've used india ink from artist supply stores for marking surgical margins, for many years, and it's entirely satisfactory. A cheap source of colored particulate inks is tattoo inks - available in a huge range of colors - I have one pathologist client who's used them for years. The only downside is that you have to read some seriously yucky catalogs. Bob Richmond Samurai Pathologist Maryville TN From nto <@t> stowers.org Thu Oct 10 12:42:42 2013 From: nto <@t> stowers.org (Thomas, Nancy) Date: Thu Oct 10 12:42:46 2013 Subject: [Histonet] RE: Novec 7100 Engineerd Fluid In-Reply-To: References: Message-ID: Hi Allison, We use Novec 7000, but we also had to switch suppliers. Now it is purchased direct through the 3M company. You will find it on their website. Nancy Thomas Stowers Institute for Medical Research -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Thursday, October 10, 2013 12:25 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Novec 7100 Engineerd Fluid Hello to all in histoland. Is there anyone using the Novec 7100 fluid for freezing frozen blocks in a low temp freezing bath. We were getting it from fisher but they have discontinued it. I am trying to find another vendor. Any help will be appreciated. Allison Scott HT(ASCP) Supervisor, Histology Lab LBJ Hospital Harris Health System Office: 713-566-2148 Lab: 713-566-5287 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Oct 10 12:45:06 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Oct 10 12:45:10 2013 Subject: [Histonet] India Ink for inking surgical margin borders In-Reply-To: <21511BF5-BA90-4392-A9B4-FF159441BC5A@cmblabs.com> References: <21511BF5-BA90-4392-A9B4-FF159441BC5A@cmblabs.com> Message-ID: <1381427106.64466.YahooMailNeo@web163104.mail.bf1.yahoo.com> India Ink = India ink, no matter where you get it from. ren? J. ________________________________ From: Ed Crespo To: histonet@lists.utsouthwestern.edu Sent: Thursday, October 10, 2013 12:53 PM Subject: [Histonet] India Ink for inking surgical margin borders I normally purchase India Ink from one of our vendors, but know it's also sold and used at artist supply shops.? Does anyone know if I can used the artist india ink for Pathology use?? Really, the only issue would be if the ink stays on the tissue during processing right? Please advise. Ed Crespo, CT(ASCP) Anatomic Pathology Manager Safety Officer 10700 Walker Street Cypress, CA 90630 phone: 714 880.3330 fax: 714 816.1511 email: ecrespo@cmblabs.com cmblabs.com The contents of this e-mail message, including any attachments, are intended solely for the use of the person or entity to whom this e-mail is addressed.? It contains information that may be privileged, proprietary, confidential, and protected from disclosure by applicable state and federal law.? Any Protected Health Information (PHI) contained in this email is HIGHLY CONFIDENTIAL.? It is intended for the exclusive use of the addressee.? It is to be used only to aid in providing specific healthcare services to the patient(s).? Any other use is a violation of Federal Law (HIPAA) and will be reported as such.? If you are not the intended recipient of this message, or the employee or agent responsible for delivering it to the intended recipient, you are hereby advised that reading, disseminating, distributing, use, or copying of the contents of this message is strictly prohibited.? If you have received this message in error, or are not the named recipient(s), please notify the sender immediately by reply e-mail or by phone at (714) 657-7369 and delete this message and any attachments from your computer and any archival/backup copies.? Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From turkekul <@t> gmail.com Thu Oct 10 13:17:47 2013 From: turkekul <@t> gmail.com (Mesru T) Date: Thu Oct 10 13:17:53 2013 Subject: [Histonet] Re: Histonet Digest, Vol 119, Issue 13 In-Reply-To: <5256dd33.019dec0a.7ce6.ffff96c3SMTPIN_ADDED_MISSING@mx.google.com> References: <5256dd33.019dec0a.7ce6.ffff96c3SMTPIN_ADDED_MISSING@mx.google.com> Message-ID: Dear Histonetters, I am so happy to discover a new CD31 antibody on mouse tissues through Histonet. It really works well. Now I need p16 antibody that is good for FFPE mouse tissues. Appreciate your help as always! Regards, Mesru mskcc.org From TGoins <@t> mt.gov Thu Oct 10 13:18:05 2013 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Thu Oct 10 13:18:19 2013 Subject: [Histonet] Re: India Ink for inking surgical margin borders In-Reply-To: References: Message-ID: Tattoos Yucky? Guess I'm yucky. I got yucky way before tattoos became popular when the response was more akin to fringe element. Happy member of the Fringe. :) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Thursday, October 10, 2013 11:43 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: India Ink for inking surgical margin borders Ed Crespo, CT(ASCP) in Cypress CA asks: >>I normally purchase India Ink from one of our vendors, but know it's also sold at artist supply shops. Does anyone know if I can use the artist india ink for Pathology? Really, the only issue would be if the ink stays on the tissue during processing right? Please advise.<< I've used india ink from artist supply stores for marking surgical margins, for many years, and it's entirely satisfactory. A cheap source of colored particulate inks is tattoo inks - available in a huge range of colors - I have one pathologist client who's used them for years. The only downside is that you have to read some seriously yucky catalogs. Bob Richmond Samurai Pathologist Maryville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From patpxs <@t> gmail.com Thu Oct 10 13:36:26 2013 From: patpxs <@t> gmail.com (Paula Sicurello) Date: Thu Oct 10 13:36:30 2013 Subject: [Histonet] External UV for a Leica 1850? Message-ID: Hello Fellow Netters, Has anyone tried using some type of external UV source to decontaminate a Leica 1850 cryostat? I found out that it is not possible to retro fit the 1850 for UV. I would like to be able to avoid having to defrost, breakdown and bleach the cryostat everytime a suspected infectious tissue is cut in it. Suggestions kindly welcomed. Thanks! Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 HIPAA Privacy Notification: This message and any accompanying documents are covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, and contain information intended for the specific individual (s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying or the taking of any action based on the contents of this information is strictly prohibited . If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From Susan.Wert <@t> cchmc.org Thu Oct 10 14:33:20 2013 From: Susan.Wert <@t> cchmc.org (Wert, Susan (Susan Wert)) Date: Thu Oct 10 14:33:26 2013 Subject: [Histonet] Fluorochrome conjugated polymers for antibody detection Message-ID: <12F351B8515AD341B90AC3D5D7480C207D9EB890@MCEXMB2.chmccorp.cchmc.org> Hello; Can anyone tell me if there are anti-rabbit polymers (secondary antibody) that are conjugated to fluorchromes ? Or are the only polymers available conjugated to HRP? Susan Wert, PhD Cincinnati Children's Hospital Research Foundation Perinatal Institute/Pulmonary Biology From BAMoe <@t> gundersenhealth.org Thu Oct 10 14:52:27 2013 From: BAMoe <@t> gundersenhealth.org (Moe, Barbi A) Date: Thu Oct 10 14:52:32 2013 Subject: [Histonet] Thermo Fisher Slide Mate printer Message-ID: <8B4B31D17067E540AC760B4A30190B99014A2C7D77@LXEXMB03.gundluth.org> If anyone is currently utilizing this printer, could you please share pros and cons of your experience? Specifically interested if anyone has the unit interfaced with Power Path computer system. Thank you! Barb Moe Gundersen Health System 1910 South Ave La Crosse WI 54601 bamoe@gundersenhealth.org From Caroline.Pratt <@t> uphs.upenn.edu Thu Oct 10 14:57:44 2013 From: Caroline.Pratt <@t> uphs.upenn.edu (Pratt, Caroline) Date: Thu Oct 10 14:57:49 2013 Subject: [Histonet] Thermo Fisher Slide Mate printer In-Reply-To: <8B4B31D17067E540AC760B4A30190B99014A2C7D77@LXEXMB03.gundluth.org> References: <8B4B31D17067E540AC760B4A30190B99014A2C7D77@LXEXMB03.gundluth.org> Message-ID: We are using them at Penn Medicine Dermatopathology. We interface with our homegrown LIS. We like the printers. We have had some ribbon issues causing the print to move and some numbers to be cut off and the ink on the fisher slide can be wiped off so we are looking at other slides to see if they are more resistant to chemicals and oils with that printer, but overall we have been happy with them. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Moe, Barbi A Sent: Thursday, October 10, 2013 3:52 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Thermo Fisher Slide Mate printer If anyone is currently utilizing this printer, could you please share pros and cons of your experience? Specifically interested if anyone has the unit interfaced with Power Path computer system. Thank you! Barb Moe Gundersen Health System 1910 South Ave La Crosse WI 54601 bamoe@gundersenhealth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From joelleweaver <@t> hotmail.com Thu Oct 10 15:09:31 2013 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Thu Oct 10 15:09:36 2013 Subject: [Histonet] Negative Reagent Controls in Diagnostic IHC.... In-Reply-To: <9215BD4B0BA1B44D962A71C758B68D2E018886EC@HHCEXCHMB05.hhcsystem.org> References: <9215BD4B0BA1B44D962A71C758B68D2E018886EC@HHCEXCHMB05.hhcsystem.org> Message-ID: I would like to see the PP slide. I was unable to attend in Providence. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: Richard.Cartun@hhchealth.org > To: histonet@lists.utsouthwestern.edu > Date: Wed, 9 Oct 2013 20:58:38 +0000 > Subject: [Histonet] Negative Reagent Controls in Diagnostic IHC.... > > My colleagues and I presented a poster at the NSH annual meeting in Providence recently titled, "Negative Reagent Controls in Diagnostic Immunohistochemistry: Do we need them?". I have received a few requests for the actual poster (PowerPoint slide). I will be happy to e-mail it to anyone who is interested. > > > > Oh, by the way, we have determined that they are not needed in our laboratory and by eliminating them we have saved our laboratory over $100,000 a year! > > > > Richard > > > > Richard W. Cartun, MS, PhD > > Director, Histology & Immunopathology > > Director, Biospecimen Collection Programs > > Assistant Director, Anatomic Pathology > > Hartford Hospital > > 80 Seymour Street > > Hartford, CT 06102 > > (860) 545-1596 Office > > (860) 545-2204 Fax > > richard.cartun@hhchealth.org > > This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PAMarcum <@t> uams.edu Thu Oct 10 15:16:31 2013 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Thu Oct 10 15:16:59 2013 Subject: [Histonet] Thermo Fisher Slide Mate printer In-Reply-To: References: <8B4B31D17067E540AC760B4A30190B99014A2C7D77@LXEXMB03.gundluth.org> Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA32D315CB17@Mail2Node2.ad.uams.edu> We use the Slide Mate and we really like them. We did have one problem with the printer tape and then we got a different lot and things have been much better. The slides we use are the Thermo Colorfrost slides, in 6 colours and we have had no issues with the ink smearing or coming off. Pam Marcum -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pratt, Caroline Sent: Thursday, October 10, 2013 2:58 PM To: Moe, Barbi A; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Thermo Fisher Slide Mate printer We are using them at Penn Medicine Dermatopathology. We interface with our homegrown LIS. We like the printers. We have had some ribbon issues causing the print to move and some numbers to be cut off and the ink on the fisher slide can be wiped off so we are looking at other slides to see if they are more resistant to chemicals and oils with that printer, but overall we have been happy with them. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Moe, Barbi A Sent: Thursday, October 10, 2013 3:52 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Thermo Fisher Slide Mate printer If anyone is currently utilizing this printer, could you please share pros and cons of your experience? Specifically interested if anyone has the unit interfaced with Power Path computer system. Thank you! Barb Moe Gundersen Health System 1910 South Ave La Crosse WI 54601 bamoe@gundersenhealth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From lpwenk <@t> sbcglobal.net Thu Oct 10 15:34:20 2013 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Thu Oct 10 15:34:28 2013 Subject: [Histonet] External UV for a Leica 1850? In-Reply-To: References: Message-ID: <0C1B8698C3104911BE3A318A4E253DAB@HP2010> If you are a CAP accredited lab, CAP says that the cryostat must be defrosted and disinfectant decontaminated at regular intervals with a TB disinfectant. - - - ANP.23410 Cryostat Decontamination Phase II There is a documented procedure for the routine decontamination of the cryostat at defined intervals, and decontamination records are evident. NOTE: The cryostat must be defrosted and decontaminated by wiping all exposed surfaces with tuberculocidal disinfectant. The cryostat should be at room temperature during decontamination unless otherwise specified by the manufacturer. This should be done at an interval appropriate for the institution; this must be weekly for instruments used daily. Trimmings and sections of tissue that accumulate inside the cryostat must be removed during decontamination. Although not a requirement, steel mesh gloves should be worn when changing knife blades. - - - - Even if you can use a UV light, ALL debris/contaminants must be removed from the cryostat chamber BEFORE using the UV light. The germicidal effect of radiation is only good on the areas that the UV light can hit directly. So any little corners, or areas under metal plates, or areas under the OCT/tissue shavings will not be directly illuminated by the UV light, and thus will not be disinfected. There are also different types of UV lamps. I have heard that low efficiency UV lamps need a long period of time of being turned on to disinfect, and that this long exposure in a small area of the chamber of the cryostat can produce a high level of ozone in the chamber, so there could be an ozone exposure level to the tech using the cryostat. So, UV light can be used in CONJUNCTION with wiping out, chemical disinfecting, and defrosting. But I don't believe it can be used IN PLACE of wiping out, chemical disinfecting, and defrosting. Peggy A. Wenk, HTL(ASCP)SLS -----Original Message----- From: Paula Sicurello Sent: Thursday, October 10, 2013 2:36 PM To: HistoNet Subject: [Histonet] External UV for a Leica 1850? Hello Fellow Netters, Has anyone tried using some type of external UV source to decontaminate a Leica 1850 cryostat? I found out that it is not possible to retro fit the 1850 for UV. I would like to be able to avoid having to defrost, breakdown and bleach the cryostat everytime a suspected infectious tissue is cut in it. Suggestions kindly welcomed. Thanks! Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 HIPAA Privacy Notification: This message and any accompanying documents are covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, and contain information intended for the specific individual (s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying or the taking of any action based on the contents of this information is strictly prohibited . If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tkngflght <@t> yahoo.com Thu Oct 10 16:24:02 2013 From: tkngflght <@t> yahoo.com (Cheryl) Date: Thu Oct 10 16:24:07 2013 Subject: [Histonet] AFB Controls Message-ID: <1381440242.51001.YahooMailNeo@web161906.mail.bf1.yahoo.com> Help? We are about to run out of AFB control slides and haven't had a good loaded case in a while. ?Is there an easy way to come up with an AFB positive block or could someone lend me one to be replaced at a later date? (Go through too many to be cost effective to buy) OR is there something out in the world I can use to make a control? Please respond to tkngflght@yahoo.com ? Cheryl Kerry, HT(ASCP) ? 281.852.9457?Office 800.756.3309?Phone & Fax? From Terra.Wineman <@t> novusint.com Thu Oct 10 16:29:51 2013 From: Terra.Wineman <@t> novusint.com (Wineman, Terra) Date: Thu Oct 10 16:29:56 2013 Subject: [Histonet] AFB Controls In-Reply-To: <1381440242.51001.YahooMailNeo@web161906.mail.bf1.yahoo.com> References: <1381440242.51001.YahooMailNeo@web161906.mail.bf1.yahoo.com> Message-ID: <1EB8F245A303564EADF12AC7022FA74D70F1AC03@NOVUS-EX01.novusint.com> Sigma provides AFB control slides if you can't get block. The item number is A2299 Terra Wineman, HTL (ASCP)CM Research Biologist 636-926-7476 phone terra.wineman@novusint.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl Sent: Thursday, October 10, 2013 4:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] AFB Controls Help? We are about to run out of AFB control slides and haven't had a good loaded case in a while. ?Is there an easy way to come up with an AFB positive block or could someone lend me one to be replaced at a later date? (Go through too many to be cost effective to buy) OR is there something out in the world I can use to make a control? Please respond to tkngflght@yahoo.com ? Cheryl Kerry, HT(ASCP) ? 281.852.9457?Office 800.756.3309?Phone & Fax? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hans <@t> histologistics.com Thu Oct 10 16:45:19 2013 From: hans <@t> histologistics.com (Hans B Snyder) Date: Thu Oct 10 16:45:26 2013 Subject: [Histonet] AFB Controls In-Reply-To: <1381440242.51001.YahooMailNeo@web161906.mail.bf1.yahoo.com> References: <1381440242.51001.YahooMailNeo@web161906.mail.bf1.yahoo.com> Message-ID: I know source medical products has them cheaper than newcomer supply. E-mail and ask for Walter. Hans B Snyder Histologistics 60 Prescott Street Worcester, MA 01605 508-308-7800 hans@histologistics.com On Thu, Oct 10, 2013 at 5:24 PM, Cheryl wrote: > Help? > > We are about to run out of AFB control slides and haven't had a good > loaded case in a while. Is there an easy way to come up with an AFB > positive block or could someone lend me one to be replaced at a later date? > (Go through too many to be cost effective to buy) > > OR is there something out in the world I can use to make a control? > > Please respond to tkngflght@yahoo.com > > Cheryl Kerry, HT(ASCP) > > 281.852.9457 Office > 800.756.3309 Phone & Fax > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From lpwenk <@t> sbcglobal.net Thu Oct 10 17:39:42 2013 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Thu Oct 10 17:39:50 2013 Subject: [Histonet] AFB Controls In-Reply-To: <1381440242.51001.YahooMailNeo@web161906.mail.bf1.yahoo.com> References: <1381440242.51001.YahooMailNeo@web161906.mail.bf1.yahoo.com> Message-ID: <8339AA3AE1AE4E37957CB38707F95315@HP2010> Make your own. Take some fresh lung (slightly edematous is better, if you can get it). Cut into 2x2 mm cubes (or largest 3x3 mm cubes). Contact Microbiology, and have them make a broth with a non-pathogenic AFB in a large tube (e.g., plastic centrifuge tube). Put lung cubes in broth. Incubate overnight (I found that room temp is usually OK). Next morning, add 10% NBF. Wait about an hour or so, then dispose of NBF, and add fresh 10% NBF. (The first NBF is diluted by the broth, and by allowing the NBF to sit in the broth for a while, it kills the bacteria. Adding the 2nd NBF allows the tissue to be fixed in 10% NBF, rather than diluted NBF.) Allow to fix most of the day, put tissue in cassettes, process as usual, embed, and you have lots of AFB controls for really cheap. Write up a cost containment (how much it cost you to make X number of blocks that you can get Y number of control slides from vs. the cost of buying the same number of Y slides from a vendor.) Management will love you for your cost containment. Works for gram +, gram -, and fungus (get the correct broth). Will work for spirochete too, but our micro lab has only a LARGE non-pathogenic spirochete, which is much larger than syphilis. So doing a Steiner stain would most likely yield a false-negative. (When the large spirochetes control is seen, the little syphilis would not be stained.) Please realize, these controls may not work for IHC. Better check on the genus. They work great for Kinyoun/Ziehl-Neelsen/Fites, Brown and Hopps/Brown and Brenn, GMS/PAS, Steiner/Warthin-Starry. Peggy Wenk, HTL(ASCP)SLS -----Original Message----- From: Cheryl Sent: Thursday, October 10, 2013 5:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] AFB Controls Help? We are about to run out of AFB control slides and haven't had a good loaded case in a while. Is there an easy way to come up with an AFB positive block or could someone lend me one to be replaced at a later date? (Go through too many to be cost effective to buy) OR is there something out in the world I can use to make a control? Please respond to tkngflght@yahoo.com Cheryl Kerry, HT(ASCP) 281.852.9457 Office 800.756.3309 Phone & Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From abeharry798 <@t> gmail.com Thu Oct 10 19:19:20 2013 From: abeharry798 <@t> gmail.com (Andrea) Date: Thu Oct 10 19:19:30 2013 Subject: [Histonet] Negative Reagent Controls in Diagnostic IHC.... In-Reply-To: <9215BD4B0BA1B44D962A71C758B68D2E018886EC@HHCEXCHMB05.hhcsystem.org> References: <9215BD4B0BA1B44D962A71C758B68D2E018886EC@HHCEXCHMB05.hhcsystem.org> Message-ID: <4DA02DF3-8E48-4808-A9E8-29CCDE2698B8@gmail.com> Hi Richard, I would appreciate a copy of the power point. thanks, Andrea On 2013-10-09, at 4:58 PM, "Cartun, Richard" wrote: > My colleagues and I presented a poster at the NSH annual meeting in Providence recently titled, "Negative Reagent Controls in Diagnostic Immunohistochemistry: Do we need them?". I have received a few requests for the actual poster (PowerPoint slide). I will be happy to e-mail it to anyone who is interested. > > > > Oh, by the way, we have determined that they are not needed in our laboratory and by eliminating them we have saved our laboratory over $100,000 a year! > > > > Richard > > > > Richard W. Cartun, MS, PhD > > Director, Histology & Immunopathology > > Director, Biospecimen Collection Programs > > Assistant Director, Anatomic Pathology > > Hartford Hospital > > 80 Seymour Street > > Hartford, CT 06102 > > (860) 545-1596 Office > > (860) 545-2204 Fax > > richard.cartun@hhchealth.org > > This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Traczyk7 <@t> aol.com Thu Oct 10 20:50:07 2013 From: Traczyk7 <@t> aol.com (Traczyk7@aol.com) Date: Thu Oct 10 20:50:12 2013 Subject: [Histonet] External UV for a Leica 1850? Message-ID: Paula, A UV insert in available from Hacker Instruments. (1-800-4-HACKER) It was not designed specifically for an 1850, but I don't see why it would not work out for you. Give Jim Mullen a call, he should be able to give you the device dimensions and power specifications. Have a great day. Dorothy Dorothy Traczyk BS, HT(ASCP) Histology Technician Monmouth Medical Center Long Branch, NJ 07740 In a message dated 10/10/2013 2:36:42 P.M. Eastern Daylight Time, patpxs@gmail.com writes: Hello Fellow Netters, Has anyone tried using some type of external UV source to decontaminate a Leica 1850 cryostat? I found out that it is not possible to retro fit the 1850 for UV. I would like to be able to avoid having to defrost, breakdown and bleach the cryostat everytime a suspected infectious tissue is cut in it. Suggestions kindly welcomed. Thanks! Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 HIPAA Privacy Notification: This message and any accompanying documents are covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, and contain information intended for the specific individual (s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying or the taking of any action based on the contents of this information is strictly prohibited . If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> gmail.com Thu Oct 10 21:27:50 2013 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Thu Oct 10 21:27:54 2013 Subject: [Histonet] Re: Novec 7100 Engineerd Fluid Message-ID: 3M? Novec? Engineered Fluid HFE-7100 is made by 3M. They should be able to tell you how to get it. I haven't had my hands on it yet for frozen sections, but it's being very highly recommended. Bob Richmond Samurai Pathologist Maryville, TN From rsrichmond <@t> gmail.com Thu Oct 10 21:36:55 2013 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Thu Oct 10 21:36:58 2013 Subject: [Histonet] Re: AFB Controls Message-ID: With due deference to Peggy Wenk, I'm a little hesitant about AFB and other bacterial and fungal controls prepared by artificially inoculating normal lung tissue, particularly with AFB other than Mycobacterium tuberculosis. I don't understand why people don't use animal material here. Surely some enterprising vendor could find a researcher who's infecting guinea pigs with Myco. tuberculosis. The best AFB control material I've ever seen came from rhesus monkeys imported from India (a practice now prohibited) for research purposes. The veterinarians tuberculin tested the monkeys, put down the ones that tested positive, and autopsied them. Perfectly preserved tissue, loaded with red bugs, and HIPAA doesn't give a hoot about monkeys. Bob Richmond Samurai Pathologist Maryville TN From rsrichmond <@t> gmail.com Thu Oct 10 21:50:04 2013 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Thu Oct 10 21:50:08 2013 Subject: [Histonet] Re: India Ink for inking surgical margin borders Message-ID: Pam Marcum notes that >>The only warning I know of (and I have used India Ink) is to be sure it is the "permanent" India Ink not the washable. If you buy the non-permanent it will come off in processing.<< India ink is a suspension of carbon black (basically soot) in a suitable vehicle. It's quite "permanent" - there is no "washable" India ink. Ask at the art supply store if you're unsure of what you're buying. if you blot the specimen thoroughly dry before you ink it, you don't need "fixatives" for the ink like acetic acid, acetone, or Bouin fixative. I never use them. Ink won't stick to a cauterized surface (like a LEEP or a lumpectomy specimen) but the pathologist can see those cauterized margins under the microscope anyway. I didn't say tattoos were yucky - I said the catalogs were yucky. But bear with an old man who doesn't think they make girls any more like they did in 1955 (fortunately I've got one). As more and more restrictions are put on the tools grossing pathologists, PAs, and technologists can have, it becomes more important to know how to obtain tools and supplies in the "real world". I can't replace my 25 year old Satterlee amputation saw, so I cut fractured femoral heads with a seven dollar hacksaw I bought at Home Depot. Bob Richmond Samurai Pathologist Maryville TN From chesarato <@t> hotmail.com Thu Oct 10 21:50:29 2013 From: chesarato <@t> hotmail.com (Cesar Francisco Romero) Date: Thu Oct 10 21:50:32 2013 Subject: [Histonet] RE: India Ink for inking surgical margin borders Message-ID: I use Artist China ( India ) Ink. The secret is to immerse the blocks in Bouin?s Fixative to coagulate the Ink. After that put the tissue blocks directly in the First Alcohol. Don?t go back to formalin. Cesar Romero Buenos Aires Argentina From Fawn.Bomar <@t> HalifaxRegional.com Fri Oct 11 06:48:52 2013 From: Fawn.Bomar <@t> HalifaxRegional.com (Fawn Bomar) Date: Fri Oct 11 06:49:12 2013 Subject: [Histonet] Validating on new Benchmark Message-ID: <0111BC10D77DC54EAB99B2DDA3BCE4B95A5F4D@EXCH-2K10.hrhs.com> Hi everyone, I have a few more questions that I was hoping I could get some help on. 1. How did everyone go about obtaining the positive and negative controls-(did you choose patient cases or go with generic controls such as extra tonsil, appendix, colon,etc...) 2. For the negative controls do you have to find separate negatives or can you use the internal negative controls if the tissue has it. 3. Do you have to do a minimum of 10 positive and negatives or can we do 5 (other than the ER/PR and Her2) Thank you Fawn ------------------------------------------------------------- This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you From tkngflght <@t> yahoo.com Fri Oct 11 07:34:23 2013 From: tkngflght <@t> yahoo.com (Cheryl) Date: Fri Oct 11 07:34:28 2013 Subject: [Histonet] AFB Control MANY THANKS! Message-ID: <1381494863.30942.YahooMailNeo@web161904.mail.bf1.yahoo.com> Many thanks to all who responded!? We'll be in good shape in a few days. ? Appreciate the depth of knowledge with which you are all so generous~~always! ? Happy Friday! ? Cheryl Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab by helping one GREAT?Tech at a time.? 281.852.9457?Office 800.756.3309?Phone & Fax? admin@fullstaff.org? From LSebree <@t> uwhealth.org Fri Oct 11 07:47:28 2013 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Fri Oct 11 07:47:36 2013 Subject: [Histonet] RE: Validating on new Benchmark In-Reply-To: <0111BC10D77DC54EAB99B2DDA3BCE4B95A5F4D@EXCH-2K10.hrhs.com> References: <0111BC10D77DC54EAB99B2DDA3BCE4B95A5F4D@EXCH-2K10.hrhs.com> Message-ID: <77DD817201982748BC67D7960F2F76AF06D272@UWHC-MBX12.uwhis.hosp.wisc.edu> Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fawn Bomar Sent: Friday, October 11, 2013 6:49 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Validating on new Benchmark Hi everyone, I have a few more questions that I was hoping I could get some help on. 1. How did everyone go about obtaining the positive and negative controls-(did you choose patient cases or go with generic controls such as extra tonsil, appendix, colon,etc...) We used both. 2. For the negative controls do you have to find separate negatives or can you use the internal negative controls if the tissue has it. Our in-house QA people determined we need to have separate negative cases. 3. Do you have to do a minimum of 10 positive and negatives or can we do 5 (other than the ER/PR and Her2) CAP is vague on this saying that for hard-to-find positive cases, less than 5 +/- will suffice. Thank you Fawn ------------------------------------------------------------- This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From billodonnell <@t> catholichealth.net Fri Oct 11 08:16:54 2013 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Fri Oct 11 08:17:16 2013 Subject: [Histonet] Re: AFB Controls In-Reply-To: References: Message-ID: The only caution I would add to animal tissues is that they should be securely stored. In 1994, a group of animal rights activists opened the slide box and set all of my control slides free. Oh, yes, it's Friday. Bill -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Thursday, October 10, 2013 9:37 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: AFB Controls With due deference to Peggy Wenk, I'm a little hesitant about AFB and other bacterial and fungal controls prepared by artificially inoculating normal lung tissue, particularly with AFB other than Mycobacterium tuberculosis. I don't understand why people don't use animal material here. Surely some enterprising vendor could find a researcher who's infecting guinea pigs with Myco. tuberculosis. The best AFB control material I've ever seen came from rhesus monkeys imported from India (a practice now prohibited) for research purposes. The veterinarians tuberculin tested the monkeys, put down the ones that tested positive, and autopsied them. Perfectly preserved tissue, loaded with red bugs, and HIPAA doesn't give a hoot about monkeys. Bob Richmond Samurai Pathologist Maryville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. From Joyce.Weems <@t> emoryhealthcare.org Fri Oct 11 08:41:21 2013 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Fri Oct 11 08:41:30 2013 Subject: [Histonet] Re: AFB Controls In-Reply-To: References: Message-ID: Well that sure helped the animals.. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Friday, October 11, 2013 9:17 AM To: Bob Richmond; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: AFB Controls The only caution I would add to animal tissues is that they should be securely stored. In 1994, a group of animal rights activists opened the slide box and set all of my control slides free. Oh, yes, it's Friday. Bill -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Thursday, October 10, 2013 9:37 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: AFB Controls With due deference to Peggy Wenk, I'm a little hesitant about AFB and other bacterial and fungal controls prepared by artificially inoculating normal lung tissue, particularly with AFB other than Mycobacterium tuberculosis. I don't understand why people don't use animal material here. Surely some enterprising vendor could find a researcher who's infecting guinea pigs with Myco. tuberculosis. The best AFB control material I've ever seen came from rhesus monkeys imported from India (a practice now prohibited) for research purposes. The veterinarians tuberculin tested the monkeys, put down the ones that tested positive, and autopsied them. Perfectly preserved tissue, loaded with red bugs, and HIPAA doesn't give a hoot about monkeys. Bob Richmond Samurai Pathologist Maryville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From ccrowder <@t> vetmed.lsu.edu Fri Oct 11 09:48:59 2013 From: ccrowder <@t> vetmed.lsu.edu (Cheryl Crowder) Date: Fri Oct 11 09:49:42 2013 Subject: [Histonet] India ink Message-ID: Recently I needed to mark some previously fixed and decaled bones. I had neither tattoo ink or India ink. As an alternative, I rinsed the tissue, blotted it dry and marked the surface with a fine point cassette/slide marker. I use both StatLab and Leica. It worked like a charm. Because the ink is permanent there was no problem losing it during processing and the fine points left just small dots. The only thing is that the tissues should be blotted well as moisture seems to cause a little bleeding. Cheryl Crowder, HTL(ASCP) From billodonnell <@t> catholichealth.net Fri Oct 11 10:02:56 2013 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Fri Oct 11 10:03:12 2013 Subject: [Histonet] CPT question Message-ID: I know that there is a technical component for histologic prep for a bone marrow aspirate and biopsy. Is there a way of charging for the technician who goes to the surgery suite or patient room to help a clinician collect the bone marrow? William (Bill) O'Donnell, HT (ASCP) QIHC Senior Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 SERENITY is not freedom from the storm, but peace amid the storm. Cultivate it in PRAYER! -----Original Message----- From: Weems, Joyce K. [mailto:Joyce.Weems@emoryhealthcare.org] Sent: Friday, October 11, 2013 8:41 AM To: O'Donnell, Bill; Bob Richmond; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: AFB Controls Well that sure helped the animals.. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Friday, October 11, 2013 9:17 AM To: Bob Richmond; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: AFB Controls The only caution I would add to animal tissues is that they should be securely stored. In 1994, a group of animal rights activists opened the slide box and set all of my control slides free. Oh, yes, it's Friday. Bill -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Thursday, October 10, 2013 9:37 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: AFB Controls With due deference to Peggy Wenk, I'm a little hesitant about AFB and other bacterial and fungal controls prepared by artificially inoculating normal lung tissue, particularly with AFB other than Mycobacterium tuberculosis. I don't understand why people don't use animal material here. Surely some enterprising vendor could find a researcher who's infecting guinea pigs with Myco. tuberculosis. The best AFB control material I've ever seen came from rhesus monkeys imported from India (a practice now prohibited) for research purposes. The veterinarians tuberculin tested the monkeys, put down the ones that tested positive, and autopsied them. Perfectly preserved tissue, loaded with red bugs, and HIPAA doesn't give a hoot about monkeys. Bob Richmond Samurai Pathologist Maryville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From Wanda.Smith <@t> HCAhealthcare.com Fri Oct 11 10:37:48 2013 From: Wanda.Smith <@t> HCAhealthcare.com (Wanda.Smith@HCAhealthcare.com) Date: Fri Oct 11 10:38:29 2013 Subject: [Histonet] India Ink for inking surgical margin borders In-Reply-To: <21511BF5-BA90-4392-A9B4-FF159441BC5A@cmblabs.com> References: <21511BF5-BA90-4392-A9B4-FF159441BC5A@cmblabs.com> Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BA27E31F7135@NADCWPMSGCMS03.hca.corpad.net> We sued India Ink from an art supply store for years. WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC? 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ed Crespo Sent: Thursday, October 10, 2013 12:53 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] India Ink for inking surgical margin borders I normally purchase India Ink from one of our vendors, but know it's also sold and used at artist supply shops. Does anyone know if I can used the artist india ink for Pathology use? Really, the only issue would be if the ink stays on the tissue during processing right? Please advise. Ed Crespo, CT(ASCP) Anatomic Pathology Manager Safety Officer 10700 Walker Street Cypress, CA 90630 phone: 714 880.3330 fax: 714 816.1511 email: ecrespo@cmblabs.com cmblabs.com The contents of this e-mail message, including any attachments, are intended solely for the use of the person or entity to whom this e-mail is addressed. It contains information that may be privileged, proprietary, confidential, and protected from disclosure by applicable state and federal law. Any Protected Health Information (PHI) contained in this email is HIGHLY CONFIDENTIAL. It is intended for the exclusive use of the addressee. It is to be used only to aid in providing specific healthcare services to the patient(s). Any other use is a violation of Federal Law (HIPAA) and will be reported as such. If you are not the intended recipient of this message, or the employee or agent responsible for delivering it to the intended recipient, you are hereby advised that reading, disseminating, distributing, use, or copying of the contents of this message is strictly prohibited. If you have received this message in error, or are not the named recipient(s), please notify the sender immediately by reply e-mail or by phone at (714) 657-7369 and delete this message and any attachments from your computer and any archival/backup copies. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Fri Oct 11 10:43:48 2013 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Fri Oct 11 10:43:53 2013 Subject: [Histonet] Re: AFB Controls In-Reply-To: References: , , Message-ID: Thanks....I needed a good giggle Joelle Weaver MAOM, HTL (ASCP) QIHC > From: Joyce.Weems@emoryhealthcare.org > To: billodonnell@catholichealth.net; rsrichmond@gmail.com; histonet@lists.utsouthwestern.edu > Date: Fri, 11 Oct 2013 13:41:21 +0000 > Subject: RE: [Histonet] Re: AFB Controls > CC: > > Well that sure helped the animals.. > > Joyce Weems > Pathology Manager > 678-843-7376 Phone > 678-843-7831 Fax > joyce.weems@emoryhealthcare.org > > > > www.saintjosephsatlanta.org > 5665 Peachtree Dunwoody Road > Atlanta, GA 30342 > > This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill > Sent: Friday, October 11, 2013 9:17 AM > To: Bob Richmond; Histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Re: AFB Controls > > The only caution I would add to animal tissues is that they should be securely stored. In 1994, a group of animal rights activists opened the slide box and set all of my control slides free. > > Oh, yes, it's Friday. > > Bill > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bob Richmond > Sent: Thursday, October 10, 2013 9:37 PM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: AFB Controls > > With due deference to Peggy Wenk, I'm a little hesitant about AFB and other bacterial and fungal controls prepared by artificially inoculating normal lung tissue, particularly with AFB other than Mycobacterium tuberculosis. > > I don't understand why people don't use animal material here. Surely some enterprising vendor could find a researcher who's infecting guinea pigs with Myco. tuberculosis. > > The best AFB control material I've ever seen came from rhesus monkeys imported from India (a practice now prohibited) for research purposes. The veterinarians tuberculin tested the monkeys, put down the ones that tested positive, and autopsied them. > > Perfectly preserved tissue, loaded with red bugs, and HIPAA doesn't give a hoot about monkeys. > > Bob Richmond > Samurai Pathologist > Maryville TN > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ________________________________ > > This e-mail message (including any attachments) is for the sole use of > the intended recipient(s) and may contain confidential and privileged > information. If the reader of this message is not the intended > recipient, you are hereby notified that any dissemination, distribution > or copying of this message (including any attachments) is strictly > prohibited. > > If you have received this message in error, please contact > the sender by reply e-mail message and destroy all copies of the > original message (including attachments). > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek <@t> digestivespecialists.com Fri Oct 11 10:44:29 2013 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Fri Oct 11 10:44:35 2013 Subject: [Histonet] India Ink for inking surgical margin borders In-Reply-To: <9E2D36CE2D7CBA4A94D9B22E8328A3BA27E31F7135@NADCWPMSGCMS03.hca.corpad.net> References: <21511BF5-BA90-4392-A9B4-FF159441BC5A@cmblabs.com> <9E2D36CE2D7CBA4A94D9B22E8328A3BA27E31F7135@NADCWPMSGCMS03.hca.corpad.net> Message-ID: <5A2BD13465E061429D6455C8D6B40E391666DE3186@IBMB7Exchange.digestivespecialists.com> LOL It's Friday............... I can't resist! You SUED India Ink? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Wanda.Smith@HCAhealthcare.com Sent: Friday, October 11, 2013 11:38 AM To: ecrespo@cmblabs.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] India Ink for inking surgical margin borders We sued India Ink from an art supply store for years. WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC? 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ed Crespo Sent: Thursday, October 10, 2013 12:53 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] India Ink for inking surgical margin borders I normally purchase India Ink from one of our vendors, but know it's also sold and used at artist supply shops. Does anyone know if I can used the artist india ink for Pathology use? Really, the only issue would be if the ink stays on the tissue during processing right? Please advise. Ed Crespo, CT(ASCP) Anatomic Pathology Manager Safety Officer 10700 Walker Street Cypress, CA 90630 phone: 714 880.3330 fax: 714 816.1511 email: ecrespo@cmblabs.com cmblabs.com The contents of this e-mail message, including any attachments, are intended solely for the use of the person or entity to whom this e-mail is addressed. It contains information that may be privileged, proprietary, confidential, and protected from disclosure by applicable state and federal law. Any Protected Health Information (PHI) contained in this email is HIGHLY CONFIDENTIAL. It is intended for the exclusive use of the addressee. It is to be used only to aid in providing specific healthcare services to the patient(s). Any other use is a violation of Federal Law (HIPAA) and will be reported as such. If you are not the intended recipient of this message, or the employee or agent responsible for delivering it to the intended recipient, you are hereby advised that reading, disseminating, distributing, use, or copying of the contents of this message is strictly prohibited. If you have received this message in error, or are not the named recipient(s), please notify the sender immediately by reply e-mail or by phone at (714) 657-7369 and delete this message and any attachments from your computer and any archival/backup copies. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From melissa <@t> alliedsearchpartners.com Fri Oct 11 11:01:43 2013 From: melissa <@t> alliedsearchpartners.com (Melissa Phelan) Date: Fri Oct 11 11:01:51 2013 Subject: [Histonet] Pathologists Assistant Needed for 3 Months in a New York Lab Message-ID: <011801cec69b$2e4557d0$8ad00770$@alliedsearchpartners.com> Hello and TGIF, I have a need for a Pathologist Assistant in a New York lab to cover for 3 months. This is an immediate need. Please contact me if this is of interest to you. Thank you! To view a complete list of Allied Search Partners current openings go to: http://www.alliedsearchpartners.com/careers.php Melissa Phelan President, Laboratory Staffing Allied Search Partners LinkedIn: www.linkedin.com/in/melissaphelan/ T: 888.388.7571 ext. 102 F: 888.388.7572 *If you wish to no longer receive emails from Allied Search Partners please respond to this email message with "remove." This email including its attachments is intended only for the confidential use of the individual to whom it is addressed. If you are not the intended recipient, any use, dissemination, distribution or copying of this message or its attachments is prohibited. If you have received this message in error, please notify us immediately, and delete this message and its attachments permanently from your system. From tgenade <@t> gmail.com Fri Oct 11 12:16:30 2013 From: tgenade <@t> gmail.com (Tyrone Genade) Date: Fri Oct 11 12:16:42 2013 Subject: [Histonet] Rankin Biomedical: reliable? Message-ID: Hello, Does anyone have any experience with the refurbished equipment sold by Rankin Biomedical? If so, please let me know privately. I need to motivate for a microtome & crypstat for the lab and since funding is tight I'm giving serious thought to going with what Rankin Biomedical has to offer. Thanks -- Tyrone Genade http://tgenade.freeshell.org email: tgenade@gmail.com tel: +27-84-632-1925 (c) ******************************************************************************** Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord. To find out how to receive this FREE gift visit http://www.alpha.org. From barryrittman <@t> gmail.com Fri Oct 11 12:20:28 2013 From: barryrittman <@t> gmail.com (Barry Rittman) Date: Fri Oct 11 12:20:36 2013 Subject: [Histonet] India Ink for inking surgical margin borders In-Reply-To: <5A2BD13465E061429D6455C8D6B40E391666DE3186@IBMB7Exchange.digestivespecialists.com> References: <21511BF5-BA90-4392-A9B4-FF159441BC5A@cmblabs.com> <9E2D36CE2D7CBA4A94D9B22E8328A3BA27E31F7135@NADCWPMSGCMS03.hca.corpad.net> <5A2BD13465E061429D6455C8D6B40E391666DE3186@IBMB7Exchange.digestivespecialists.com> Message-ID: One group of dyes that I do not believe has ben mentioned is the Procion group of dyes. These are chloro-s-triazone dyes that are used extensively in textile dyeing and are resistant to all the solvents of which I am aware and will survive processing through paraffin wax. Readily available at many art stores. They come in a wide variety of colors . They interact with proteins. Their drawback is that many are fluorescent (and were originally intended as markers for hard tissue growth due to their solvent resistance). Barry On Fri, Oct 11, 2013 at 10:44 AM, Blazek, Linda < lblazek@digestivespecialists.com> wrote: > LOL It's Friday............... I can't resist! You SUED India Ink? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Wanda.Smith@HCAhealthcare.com > Sent: Friday, October 11, 2013 11:38 AM > To: ecrespo@cmblabs.com; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] India Ink for inking surgical margin borders > > We sued India Ink from an art supply store for years. > > WANDA G. SMITH, HTL(ASCP)HT > Pathology Supervisor > TRIDENT MEDICAL CENTER > 9330 Medical Plaza Drive > Charleston, SC 29406 > 843-847-4586 > 843-847-4296 fax > > This email and any files transmitted with it may contain PRIVILEGED or > CONFIDENTIAL information and may be read or used only by the intended > recipient. If you are not the intended recipient of the email or any of its > attachments, please be advised that you have received this email in error > and that any use, dissemination, distribution, forwarding, printing, or > copying of this email or any attached files is strictly prohibited. If you > have received this email in error, please immediately purge it and all > attachments and notify the sender by reply email or contact the sender at > the number listed. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ed Crespo > Sent: Thursday, October 10, 2013 12:53 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] India Ink for inking surgical margin borders > > I normally purchase India Ink from one of our vendors, but know it's also > sold and used at artist supply shops. Does anyone know if I can used the > artist india ink for Pathology use? Really, the only issue would be if the > ink stays on the tissue during processing right? Please advise. > > Ed Crespo, CT(ASCP) > Anatomic Pathology Manager > Safety Officer > > > 10700 Walker Street > Cypress, CA 90630 > phone: 714 880.3330 > fax: 714 816.1511 > email: ecrespo@cmblabs.com > cmblabs.com > > > The contents of this e-mail message, including any attachments, are > intended solely for the use of the person or entity to whom this e-mail is > addressed. It contains information that may be privileged, proprietary, > confidential, and protected from disclosure by applicable state and federal > law. Any Protected Health Information (PHI) contained in this email is > HIGHLY CONFIDENTIAL. It is intended for the exclusive use of the > addressee. It is to be used only to aid in providing specific healthcare > services to the patient(s). Any other use is a violation of Federal Law > (HIPAA) and will be reported as such. If you are not the intended > recipient of this message, or the employee or agent responsible for > delivering it to the intended recipient, you are hereby advised that > reading, disseminating, distributing, use, or copying of the contents of > this message is strictly prohibited. If you have received this message in > error, or are not the named recipient(s), please notify the sender > immediately by reply e-mail or by phone at (714) 657-7369 and delete this > message and any attachments from your computer and any archival/backup > copies. Thank you. > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From dreynold <@t> mdanderson.org Fri Oct 11 13:53:41 2013 From: dreynold <@t> mdanderson.org (Reynolds,Donna M) Date: Fri Oct 11 13:54:44 2013 Subject: [Histonet] RE: Vol 119, Issue 14 polymer flurochromes Message-ID: <01C7405EC5F26E40A9A64015F13B461C01889545@D1PWPEXMBX04.mdanderson.edu> About 3 years ago I ask a vendor who was selling polymers for animal tissue if I could get it with fluchromes. He told me it couldn't be done. I haven't seen any on the market so I am guessing no one has figured out how to do it. It would sure be nice for research work. Donna Reynolds HT(ASCP) Chief Histo Tech, IHC Lab Dept. Cancer Biology, U T M D. Anderson Cancer Center 713 794-1066 ------------------------------ Message: 9 Date: Thu, 10 Oct 2013 19:33:20 +0000 From: "Wert, Susan (Susan Wert)" Subject: [Histonet] Fluorochrome conjugated polymers for antibody detection To: "histonet@lists.utsouthwestern.edu" Message-ID: <12F351B8515AD341B90AC3D5D7480C207D9EB890@MCEXMB2.chmccorp.cchmc.org> Content-Type: text/plain; charset="us-ascii" Hello; Can anyone tell me if there are anti-rabbit polymers (secondary antibody) that are conjugated to fluorchromes ? Or are the only polymers available conjugated to HRP? Susan Wert, PhD Cincinnati Children's Hospital Research Foundation Perinatal Institute/Pulmonary Biology ------------------------------ * From lu_ze <@t> sbcglobal.net Fri Oct 11 14:37:50 2013 From: lu_ze <@t> sbcglobal.net (Lu Ze) Date: Fri Oct 11 14:37:54 2013 Subject: [Histonet] Mouse lung IHC false positive/background issue Message-ID: <1381520270.88478.YahooMailNeo@web181304.mail.ne1.yahoo.com> Hello everyone, I forwarded message from my colleague, Rebecca (see below) to see if any one with IHC experience on mouse lung tissue have suggestion. =========================================================================================== I'm having trouble with my IHC. I am staining for human MTDH (specific for human) on mouse tumor and lung sections from my xenograft mouse model. All of my tumors stained positive, but not the lung sections (which could possibly be the results). My real problem is that my negative control normal lung section stained positive for MTDH. I saw positive staining on the tissue near the outside and in the bronchioles. I know the problem is not the secondary antibody because I did 2 slides (normal lung and previously positive tumor) without primary ab and they showed no positive staining. My antibody is a rabbit monoclonal antibody at 1:200 for 1 hour. I'm blocking with 1% BSA in TBST for 1 hour. I'm using DAB substrate kit for developing. What would you recommend? Thank you, Ze ================================ Ze Lu, Ph.D. Optimum Therapeutics, LLC 9363 Towne Centre Dr., Suite 110 San Diego, CA 92121 From NMargaryan <@t> luriechildrens.org Fri Oct 11 15:01:55 2013 From: NMargaryan <@t> luriechildrens.org (Margaryan, Naira) Date: Fri Oct 11 15:01:59 2013 Subject: [Histonet] Mouse lung IHC false positive/background issue In-Reply-To: <1381520270.88478.YahooMailNeo@web181304.mail.ne1.yahoo.com> References: <1381520270.88478.YahooMailNeo@web181304.mail.ne1.yahoo.com> Message-ID: <34B2EDA118548A4EB35D6E650345BA6431E67859@SV-EX08.childrensmemorial.org> I have same problem for other Abs using in on mouse lung tissues... My secondary and tertiary have no problems on other tissues for negative controls as well.... Naira ************** Confidentiality Note: This message (including any attachments) is intended for the use of the named recipient(s) only and may contain confidential and/or proprietary information. If you are not the intended recipient, please contact the sender and delete this message. Any unauthorized use of the information contained in this message is prohibited. Ranked nationally in all 10 pediatric specialties by U.S. News & World Report (LCHOC Ver 1.0) This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. If verification is required please request a hard-copy version. (LCHOC VER 1.0) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lu Ze Sent: Friday, October 11, 2013 2:38 PM To: histonet@lists.utsouthwestern.edu Cc: rebeliza@gmail.com Subject: [Histonet] Mouse lung IHC false positive/background issue Hello everyone, I forwarded message from my colleague, Rebecca (see below) to see if any one with IHC experience on mouse lung tissue have suggestion. =========================================================================================== I'm having trouble with my IHC. I am staining for human MTDH (specific for human) on mouse tumor and lung sections from my xenograft mouse model. All of my tumors stained positive, but not the lung sections (which could possibly be the results). My real problem is that my negative control normal lung section stained positive for MTDH. I saw positive staining on the tissue near the outside and in the bronchioles. I know the problem is not the secondary antibody because I did 2 slides (normal lung and previously positive tumor) without primary ab and they showed no positive staining. My antibody is a rabbit monoclonal antibody at 1:200 for 1 hour. I'm blocking with 1% BSA in TBST for 1 hour. I'm using DAB substrate kit for developing. What would you recommend? Thank you, Ze ================================ Ze Lu, Ph.D. Optimum Therapeutics, LLC 9363 Towne Centre Dr., Suite 110 San Diego, CA 92121 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From one_angel_secret <@t> yahoo.com Fri Oct 11 15:46:44 2013 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Fri Oct 11 15:48:01 2013 Subject: [Histonet] Rankin Biomedical: reliable? In-Reply-To: References: Message-ID: <294D39E2-5B8C-4702-824C-4BAAC0AFF651@yahoo.com> Love them I've bought a few things from them Sent from my iPhone On Oct 11, 2013, at 1:16 PM, Tyrone Genade wrote: > Hello, > > Does anyone have any experience with the refurbished equipment sold by Rankin > Biomedical? If so, please let me know privately. > > I need to motivate for a microtome & crypstat for the lab and since funding > is tight I'm giving serious thought to going with what Rankin Biomedical > has to offer. > > Thanks > -- > Tyrone Genade > http://tgenade.freeshell.org > email: tgenade@gmail.com > tel: +27-84-632-1925 (c) > ******************************************************************************** > Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord. > To find out how to receive this FREE gift visit http://www.alpha.org. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tjey <@t> hotmail.com Fri Oct 11 16:54:52 2013 From: tjey <@t> hotmail.com (Tanya Ewing-Finchem) Date: Fri Oct 11 16:54:58 2013 Subject: [Histonet] Negative Reagent Controls in Diagnostic IHC In-Reply-To: References: Message-ID: The Negative side of NO Negative controls?" An interesting paper on the discussion around Negative controls. Here is the link: http://www.darkdaily.com/white-papers/identifying-false-positive-results-in-immunohistochemical-assays-the-value-of-negative-reagent-controls-in-the-clinical-laboratory-93013#ixzz2gg8GjvIA > Message: 3 > Date: Wed, 9 Oct 2013 20:58:38 +0000 > From: "Cartun, Richard" > Subject: [Histonet] Negative Reagent Controls in Diagnostic IHC.... > To: Histonet > Message-ID: > <9215BD4B0BA1B44D962A71C758B68D2E018886EC@HHCEXCHMB05.hhcsystem.org> > Content-Type: text/plain; charset="iso-8859-1" > > My colleagues and I presented a poster at the NSH annual meeting in Providence recently titled, "Negative Reagent Controls in Diagnostic Immunohistochemistry: Do we need them?". I have received a few requests for the actual poster (PowerPoint slide). I will be happy to e-mail it to anyone who is interested. > > > > Oh, by the way, we have determined that they are not needed in our laboratory and by eliminating them we have saved our laboratory over $100,000 a year! > > > > Richard > > > > Richard W. Cartun, MS, PhD > > Director, Histology & Immunopathology > > Director, Biospecimen Collection Programs > > Assistant Director, Anatomic Pathology > > Hartford Hospital > > 80 Seymour Street > > Hartford, CT 06102 > > (860) 545-1596 Office > > (860) 545-2204 Fax > > richard.cartun@hhchealth.org > > This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. > > ************* From pruegg <@t> ihctech.net Fri Oct 11 20:28:25 2013 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Fri Oct 11 20:28:35 2013 Subject: [Histonet] Mouse lung IHC false positive/background issue In-Reply-To: <34B2EDA118548A4EB35D6E650345BA6431E67859@SV-EX08.childrensmemorial.org> References: <1381520270.88478.YahooMailNeo@web181304.mail.ne1.yahoo.com>, <34B2EDA118548A4EB35D6E650345BA6431E67859@SV-EX08.childrensmemorial.org> Message-ID: if any part of your detection is something anti mouse you will get binding to all mouse tissue Ig not just the Ig associated with the antibody u are using. What is the host species of the antibody being used? Most labeled polymer detection systems use a link step which is usually rab anti mouse for mouse antibodies to link the rab portion to the labeled polymer 3rd step which is usually goat anti rab if you are using a rab antibody on mouse tissue u can skip the link step and go directly to the goat anti rab labeled polymer, avoiding anti mouse reagents. From: NMargaryan@luriechildrens.org To: lu_ze@sbcglobal.net; histonet@lists.utsouthwestern.edu Date: Fri, 11 Oct 2013 20:01:55 +0000 Subject: RE: [Histonet] Mouse lung IHC false positive/background issue CC: rebeliza@gmail.com I have same problem for other Abs using in on mouse lung tissues... My secondary and tertiary have no problems on other tissues for negative controls as well.... Naira ************** Confidentiality Note: This message (including any attachments) is intended for the use of the named recipient(s) only and may contain confidential and/or proprietary information. If you are not the intended recipient, please contact the sender and delete this message. Any unauthorized use of the information contained in this message is prohibited. Ranked nationally in all 10 pediatric specialties by U.S. News & World Report (LCHOC Ver 1.0) This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. If verification is required please request a hard-copy version. (LCHOC VER 1.0) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lu Ze Sent: Friday, October 11, 2013 2:38 PM To: histonet@lists.utsouthwestern.edu Cc: rebeliza@gmail.com Subject: [Histonet] Mouse lung IHC false positive/background issue Hello everyone, I forwarded message from my colleague, Rebecca (see below) to see if any one with IHC experience on mouse lung tissue have suggestion. =========================================================================================== I'm having trouble with my IHC. I am staining for human MTDH (specific for human) on mouse tumor and lung sections from my xenograft mouse model. All of my tumors stained positive, but not the lung sections (which could possibly be the results). My real problem is that my negative control normal lung section stained positive for MTDH. I saw positive staining on the tissue near the outside and in the bronchioles. I know the problem is not the secondary antibody because I did 2 slides (normal lung and previously positive tumor) without primary ab and they showed no positive staining. My antibody is a rabbit monoclonal antibody at 1:200 for 1 hour. I'm blocking with 1% BSA in TBST for 1 hour. I'm using DAB substrate kit for developing. What would you recommend? Thank you, Ze ================================ Ze Lu, Ph.D. Optimum Therapeutics, LLC 9363 Towne Centre Dr., Suite 110 San Diego, CA 92121 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> gmail.com Sat Oct 12 12:15:08 2013 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Sat Oct 12 12:15:14 2013 Subject: [Histonet] Re: India Ink for marking surgical margin borders Message-ID: Barry Rittman observes: >>One group of dyes that I do not believe has ben mentioned is the Procion group of dyes. These are chloro-s-triazone dyes that are used extensively in textile dyeing and are resistant to all the solvents of which I am aware and will survive processing through paraffin wax. Readily available at many art stores. They come in a wide variety of colors. They interact with proteins. Their drawback is that many are fluorescent (and were originally intended as markers for hard tissue growth due to their solvent resistance).<< But if they aren't in insoluble particles, then I won't be able to see them under the microscope! Bob Richmond Samurai Pathologist Maryville TN From histotalk <@t> yahoo.com Sat Oct 12 14:17:36 2013 From: histotalk <@t> yahoo.com (David Kemler) Date: Sat Oct 12 14:17:41 2013 Subject: [Histonet] PEGGY WENK HistoTALKS Special Guest Message-ID: <1381605456.83805.YahooMailNeo@web121502.mail.ne1.yahoo.com> Hello Everyone - ? PEGGY WENK joins us for tomorrow night's?HistoTALK show http://www.histotalk.com/. Peggy has been a guest on the show before to talk about safety. On tomorrow's 40th program, also the NSH, GSH and FSH's?40th Anniversary, Peggy shares with us her personal and professional experiences with breast cancer. A must listen, especially since October is Breast Cancer Awareness Month. ? Yours, Dave From tgenade <@t> gmail.com Sat Oct 12 15:08:56 2013 From: tgenade <@t> gmail.com (Tyrone Genade) Date: Sat Oct 12 15:09:01 2013 Subject: [Histonet] Re: Rankin Biomedical: reliable? Message-ID: Hello, Thanks, everyone, who emailed me regarding Rankin. No negative reviews. :-) Thanks very much From barryrittman <@t> gmail.com Sun Oct 13 07:22:17 2013 From: barryrittman <@t> gmail.com (Barry Rittman) Date: Sun Oct 13 07:22:26 2013 Subject: [Histonet] Re: India Ink for marking surgical margin borders In-Reply-To: References: Message-ID: Bob Good point - it is true that they are not in particles when used as a solution - but the colors can also be seen by visible light as well as by fluorescence. When I used to mark biopsy specimens it was mainly to allow correct orientation of the block in the wax and to allow the marked area to be seen when orienting and cutting the block rather than visualizing in a section. Barry On Sat, Oct 12, 2013 at 12:15 PM, Bob Richmond wrote: > Barry Rittman observes: >>One group of dyes that I do not believe has ben > mentioned is the Procion group of dyes. These are chloro-s-triazone dyes > that are used extensively in textile dyeing and are resistant to all the > solvents of which I am aware and will survive processing through paraffin > wax. Readily available at many art stores. They come in a wide variety of > colors. They interact with proteins. Their drawback is that many are > fluorescent (and were originally intended as markers for hard tissue growth > due to their solvent resistance).<< > > But if they aren't in insoluble particles, then I won't be able to see them > under the microscope! > > Bob Richmond > Samurai Pathologist > Maryville TN > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From turkekum <@t> mskcc.org Sun Oct 13 07:58:31 2013 From: turkekum <@t> mskcc.org (Turkekul, Mesruh/Sloan-Kettering Institute) Date: Sun Oct 13 07:58:41 2013 Subject: [Histonet] Re: Histonet Digest, Vol 119, Issue 16 In-Reply-To: <52598043.0880b60a.2052.ffff8817SMTPIN_ADDED_MISSING@mx.google.com> References: <52598043.0880b60a.2052.ffff8817SMTPIN_ADDED_MISSING@mx.google.com> Message-ID: Hi Histonetters, There is a company that makes polymers for Immunofluorescence. Please visit www.maxvisionbio.com Mesru From ibernard <@t> uab.edu Sun Oct 13 08:57:07 2013 From: ibernard <@t> uab.edu (Ian R Bernard) Date: Sun Oct 13 08:57:22 2013 Subject: [Histonet] Cytopathology Quest. Message-ID: Per text books, for cytology specimens, 95% Ethyl Alcohol is the fixative of choice. Are there any studies or references that approve 95% isopropyl or denatured alcohol as a suitable substitute? Ian Bernard From rsrichmond <@t> gmail.com Sun Oct 13 12:56:05 2013 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Sun Oct 13 12:56:15 2013 Subject: [Histonet] Re: India Ink for marking surgical margin borders Message-ID: Barry Rittman observes: >>When I used to mark biopsy specimens it was mainly to allow correct orientation of the block in the wax and to allow the marked area to be seen when orienting and cutting the block rather than visualizing in a section.<< Good point I hadn't thought of. In surgical pathology, if the embedder needs to see the ink, than the pathologist needs to see it too. That wouldn't be true for all research applications. Bob Richmond Samurai Pathologist Maryville TN From afimbres <@t> uci.edu Mon Oct 14 11:58:05 2013 From: afimbres <@t> uci.edu (Fimbres, Amber) Date: Mon Oct 14 11:58:09 2013 Subject: [Histonet] Cytopathology Quest. In-Reply-To: References: Message-ID: According to Koss' Diagnostic Cytology and its Histopathologic Bases (1979), 100% methanol is equivalent to 95% ethanol which is equivalent to 95% reagent (denatured) alcohol which is equivalent to 80% propanol which is equivalent to 80% isopropanol. So in theory, if you ran out of 95% reagent alcohol and only had isopropanol on hand, as long as it was 80% it would be OK to use. Have I tried this? Honestly...no. Amber Fimbres, CT(ASCP)HTL -----Original Message----- From: Ian R Bernard [mailto:ibernard@uab.edu] Sent: Sunday, October 13, 2013 6:57 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cytopathology Quest. Per text books, for cytology specimens, 95% Ethyl Alcohol is the fixative of choice. Are there any studies or references that approve 95% isopropyl or denatured alcohol as a suitable substitute? Ian Bernard ________________________________ This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. From ibernard <@t> uab.edu Mon Oct 14 15:52:25 2013 From: ibernard <@t> uab.edu (Ian R Bernard) Date: Mon Oct 14 15:52:33 2013 Subject: [Histonet] Cytopathology Quest. In-Reply-To: References: Message-ID: Thanks for your reference and input IB -----Original Message----- From: Fimbres, Amber [mailto:afimbres@uci.edu] Sent: Monday, October 14, 2013 11:58 AM To: Ian R Bernard; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Cytopathology Quest. According to Koss' Diagnostic Cytology and its Histopathologic Bases (1979), 100% methanol is equivalent to 95% ethanol which is equivalent to 95% reagent (denatured) alcohol which is equivalent to 80% propanol which is equivalent to 80% isopropanol. So in theory, if you ran out of 95% reagent alcohol and only had isopropanol on hand, as long as it was 80% it would be OK to use. Have I tried this? Honestly...no. Amber Fimbres, CT(ASCP)HTL -----Original Message----- From: Ian R Bernard [mailto:ibernard@uab.edu] Sent: Sunday, October 13, 2013 6:57 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cytopathology Quest. Per text books, for cytology specimens, 95% Ethyl Alcohol is the fixative of choice. Are there any studies or references that approve 95% isopropyl or denatured alcohol as a suitable substitute? Ian Bernard ________________________________ This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. From sadey <@t> hotmail.ca Mon Oct 14 18:21:58 2013 From: sadey <@t> hotmail.ca (Sheila Adey) Date: Mon Oct 14 18:22:03 2013 Subject: [Histonet] Standardized Histo Requisitions Message-ID: Hi Everyone:We have issues with our requisitions not being filled in specifically. I'm considering building a standardized Histo req for Dermatology, Endo and the OR.Does anyone already have one in place that they would like to share? ThanksSheila From bethcoxx <@t> gmail.com Tue Oct 15 12:47:22 2013 From: bethcoxx <@t> gmail.com (Beth Cox) Date: Tue Oct 15 12:47:28 2013 Subject: [Histonet] Re: Cytopathology Quest - alcohols In-Reply-To: <525d74c4.09b5b60a.670d.ffffd1d3SMTPIN_ADDED_MISSING@mx.google.com> References: <525d74c4.09b5b60a.670d.ffffd1d3SMTPIN_ADDED_MISSING@mx.google.com> Message-ID: <525D7FAA.3010004@gmail.com> Regarding alcohols in cytology: Amber, you are right about those alcohols being equivalents for *shrinkage rates* (and since size is one of the criteria for cyto, shrinkage rates are important) but that does NOT indicate they perform the same. Reagent alcohol (but not all other denatured alcohols) has very similar performance and shrinkage to ethyl alcohol and is considered an good acceptable substitute. 80% isopropal alcohol has similar shrinkage but not quite the same performance as ethyl and is considered an adequate substitute for fixation in emergency situations - the same is true for absolute methyl alcohol. Beth Cox, SCT/HTL(ASCP)QIHC > ---------------------------------------------------------------------- > > Message: 1 > Date: Mon, 14 Oct 2013 20:52:25 +0000 > From: Ian R Bernard > Subject: RE: [Histonet] Cytopathology Quest. > To: "Fimbres, Amber" , > "histonet@lists.utsouthwestern.edu" > > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > Thanks for your reference and input > IB > > -----Original Message----- > From: Fimbres, Amber [mailto:afimbres@uci.edu] > Sent: Monday, October 14, 2013 11:58 AM > To: Ian R Bernard; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Cytopathology Quest. > > According to Koss' Diagnostic Cytology and its Histopathologic Bases (1979), 100% methanol is equivalent to 95% ethanol which is equivalent to 95% reagent (denatured) alcohol which is equivalent to 80% propanol which is equivalent to 80% isopropanol. So in theory, if you ran out of 95% reagent alcohol and only had isopropanol on hand, as long as it was 80% it would be OK to use. Have I tried this? Honestly...no. > > Amber Fimbres, CT(ASCP)HTL > > -----Original Message----- > From: Ian R Bernard [mailto:ibernard@uab.edu] > Sent: Sunday, October 13, 2013 6:57 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Cytopathology Quest. > > Per text books, for cytology specimens, 95% Ethyl Alcohol is the fixative of choice. Are there any studies or references that approve 95% isopropyl or denatured alcohol as a suitable substitute? > > Ian Bernard From allyse124 <@t> gmail.com Tue Oct 15 13:43:32 2013 From: allyse124 <@t> gmail.com (Allyse Mazzarelli) Date: Tue Oct 15 13:43:37 2013 Subject: [Histonet] Autofluorescence on Retina Tissue Message-ID: Hi all! Question for all you that may be more immuno-experienced than I: I've consistently run immunofluorescence on pig retina and I seem to have a severe case of autofluorescence/background. I've played around with primary and secondary antibody ratios, but that doesn't seem to help my case. The primary antibodies I use are goat-anti-FLT.1, and mouse-anti-rhodopsin. The secondary antibodies I use are AlexaFluor donkey-anti-goat488 & rabbit-anti-mouse555 (For my experiments, each slide contained only one primary antibody, and it's corresponding secondary. For imaging purposes, I did not double-label on these slides. E.g. FLT.1 was labeled with the 488 secondary, and rhodopsin was labeled with the 555 secondary).I re-hydrate, conduct antigen retrieval, and block as per normal IHC protocol. However, when imaging, I noticed that both slides, labeled with either rhodopsin or FLT.1 seem to "bleed" through to the next filter. For example, mouse-anti-rhodopsin labeled with the 555 secondary works beautifully at a 1:600 ratio. However, when I switch to the FITC filter on my scope, all the retinal tissue appears green on the slides, even though it has ONLY the 555 secondary and NO 488. I've noticed this for the FLT.1 antibody as well (i.e. switch to red filter and tissue fluoresces even though no slide saw the 555 secondary antibody). As I mentioned, I decreased the ratios of all antibodies, but that still doesn't eliminate the problem. If anyone has any ideas as to how I go about fixing this, please let me know. I've only been in the field for a very short period of time, so if I missed something in my description, don't hesitate to ask! Thanks for whatever help you can direct my way! From billodonnell <@t> catholichealth.net Wed Oct 16 10:51:02 2013 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Wed Oct 16 10:51:25 2013 Subject: [Histonet] Tissue marking dyes Message-ID: I am wanting to 'cut' the dense tissue dye(s) by TBS (probably same as Cancer Diagnostics or any other). Orange is very thick, so is green. I'm being a bit lazy here, but I was wondering if anyone else is going this? Do you use water, alcohol or H2O2? Any direction would be helpful. Thanks - Bill William (Bill) O'Donnell, HT (ASCP) QIHC Senior Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 SERENITY is not freedom from the storm, but peace amid the storm. Cultivate it in PRAYER! This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. From ASelf <@t> georgetownhospitalsystem.org Wed Oct 16 11:08:21 2013 From: ASelf <@t> georgetownhospitalsystem.org (Amy Self) Date: Wed Oct 16 11:08:29 2013 Subject: [Histonet] Histology Assistant Job Description Message-ID: Happy Wednesday Everyone, Does anyone have a histology assistant job description that they could share with me? Thanks in advance for your help, Amy Self Histology Lab Senior Tech Lab Georgetown Memorial Hospital 606 Black River Road Georgetown, SC 29440 843-520-8711 ASelf@georgetownhospitalsystem.org NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. From histo <@t> pathlab.us Wed Oct 16 11:46:57 2013 From: histo <@t> pathlab.us (Histology) Date: Wed Oct 16 11:47:26 2013 Subject: [Histonet] manual IHC staining Message-ID: <9C0B151B30AC5F4ABFD1B69D915F84CF33EDEC@dc01.DominionPath.local> Hi all- Does anyone out there still do manual, offline IHC staining? We currently do about 20,000 IHC slides per year and my boss would like to consider dropping the automatic stainers and doing them by hand. If you do manual staining, how long does it take, how much tech time is needed, and what about CAP inspections? Thanks in advance, Mehndi Helgren Dominion Pathology Laboratories 733 Boush St. Suite 200 Norfolk, VA 23510 757-664-7901 histo@pathlab.us From Fawn.Bomar <@t> HalifaxRegional.com Wed Oct 16 11:52:32 2013 From: Fawn.Bomar <@t> HalifaxRegional.com (Fawn Bomar) Date: Wed Oct 16 11:52:39 2013 Subject: [Histonet] Old equipment Message-ID: <0111BC10D77DC54EAB99B2DDA3BCE4B95FFADA@EXCH-2K10.hrhs.com> Would anyone be interested in purchasing some old equipment? We have 2 Ventana Benchmarks that will be available in late November/ early December and a Leica RM 2155 microtome. The Benchmarks work, my company just upgraded to the Benchmark XT. The Leica microtome was having some issues but we couldn't get service on it so we bought a new one. Thank you Fawn Bomar ------------------------------------------------------------- This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you From rjbuesa <@t> yahoo.com Wed Oct 16 12:21:45 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Oct 16 12:21:50 2013 Subject: [Histonet] manual IHC staining In-Reply-To: <9C0B151B30AC5F4ABFD1B69D915F84CF33EDEC@dc01.DominionPath.local> References: <9C0B151B30AC5F4ABFD1B69D915F84CF33EDEC@dc01.DominionPath.local> Message-ID: <1381944105.83753.YahooMailNeo@web163103.mail.bf1.yahoo.com> There is no problem with CAP inspections if you do them manually. The tech time is the same for HIER, but it increases for the actual procedure. Please go to http://www.histosearch.com/rene.html and you will find times for manual IHC Generally speaking it will be around 10 mins. per slide per procedure. Ren? J. ________________________________ From: Histology To: histonet@lists.utsouthwestern.edu Sent: Wednesday, October 16, 2013 12:46 PM Subject: [Histonet] manual IHC staining Hi all- Does anyone out there still do manual, offline IHC staining?? We currently do about 20,000 IHC slides per year and my boss would like to consider dropping the automatic stainers and doing them by hand. If you do manual staining, how long does it take, how much tech time is needed, and what about CAP inspections?? Thanks in advance, Mehndi Helgren Dominion Pathology Laboratories 733 Boush St. Suite 200 Norfolk, VA? 23510 757-664-7901 histo@pathlab.us _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tgenade <@t> gmail.com Wed Oct 16 12:29:22 2013 From: tgenade <@t> gmail.com (Tyrone Genade) Date: Wed Oct 16 12:29:30 2013 Subject: [Histonet] Re: Histonet Digest, Vol 119, Issue 20 In-Reply-To: <525ec61b.a355b60a.7731.ffffc793SMTPIN_ADDED_MISSING@mx.google.com> References: <525ec61b.a355b60a.7731.ffffc793SMTPIN_ADDED_MISSING@mx.google.com> Message-ID: Hello Allyse, Some questions to your question: > Date: Tue, 15 Oct 2013 14:43:32 -0400 > From: Allyse Mazzarelli > Subject: [Histonet] Autofluorescence on Retina Tissue > I've consistently run immunofluorescence on pig retina and I seem to have a > severe case of autofluorescence/background. How are you fixing the tissue? Are you using NH4Cl, 0.1 M glycine etc... to quench auto-fluorescence? Where do you see the auto-florescence? Which layers of the retina? Do you have a negative control slides (no primaries or secondaries as well as no primaries with secondaries)? What do they look like? What type of microscope are you using? Confocal? Are you using a nuclear stain such as hoechst? In my experience there is normally a lot of auto-fluorescence in the retina to start with. Fixation tends to make this worse... Using 0.1 M glycine helped me a lot in reducing this problem but the retinal pigment epithelium layer and the chorion behind it would still auto-fluoresce terribly. The former is due to lipofuscin so maybe a H2O2 blocking step may take care of it... Anyone tried? But now I digress... Unless you have negative controls you don't know what the source of the auto-fluorescence is. Do you have controls, what do they look like? I was lucky, as I was working with a confocal and could tune out a lot of the background fluorescence. If you work with a normal fluorescence microscope you may have issues, especially if you have tick sections. IF you are using hoechst as a counter stain for nuclei you may be using too much of the dye. I had to use as little as 0.5 ug/mL for fixed section before I stopped getting bleed-through into other channels from the Hoechst. It showed up in the green and red channels. Hope this helps... -- Tyrone Genade http://tgenade.freeshell.org email: tgenade@gmail.com tel: +27-84-632-1925 (c) ******************************************************************************** Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord. To find out how to receive this FREE gift visit http://www.alpha.org. From SHUNTER <@t> beaumont.edu Wed Oct 16 12:37:57 2013 From: SHUNTER <@t> beaumont.edu (Sue Hunter) Date: Wed Oct 16 12:38:02 2013 Subject: [Histonet] RE: manual IHC staining In-Reply-To: <9C0B151B30AC5F4ABFD1B69D915F84CF33EDEC@dc01.DominionPath.local> References: <9C0B151B30AC5F4ABFD1B69D915F84CF33EDEC@dc01.DominionPath.local> Message-ID: Wow - I can't imagine wanting to go to manual staining for 20,000 slides per year. That's about 80-100 per day. We used to do manual staining but only about 20-40 slides per day. Unless you are running a very limited panel of antibodies, you will be hopping to keep up with all the steps. I wouldn't think you would be batching all slides in one run, so you will have timers going off all the time. You or your techs will not have time to do anything else but stain, wash, apply reagents, wash, etc. You might want to do some trial runs for timing, work flows etc before you make the decision to ditch the autostainer. I would also consider consistency of staining , correct antibodies being added to the correct slides, and amount of reagents (no matter how careful you are, you will put more on than the stainer). Just my thoughts. We do 30-35,000 slides per year, we have three Ventana Ultras and they are busy all the time. and so are my 2 techs. Sue Sue Hunter, Supervisor Advanced Diagnostics Beaumont Health System Royal Oak MI 248-898-5146 shunter@beaumont.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Histology Sent: Wednesday, October 16, 2013 12:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] manual IHC staining Hi all- Does anyone out there still do manual, offline IHC staining? We currently do about 20,000 IHC slides per year and my boss would like to consider dropping the automatic stainers and doing them by hand. If you do manual staining, how long does it take, how much tech time is needed, and what about CAP inspections? Thanks in advance, Mehndi Helgren Dominion Pathology Laboratories 733 Boush St. Suite 200 Norfolk, VA 23510 757-664-7901 histo@pathlab.us _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Wed Oct 16 12:29:28 2013 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Wed Oct 16 12:54:33 2013 Subject: [Histonet] RE: manual IHC staining In-Reply-To: <9C0B151B30AC5F4ABFD1B69D915F84CF33EDEC@dc01.DominionPath.local> References: <9C0B151B30AC5F4ABFD1B69D915F84CF33EDEC@dc01.DominionPath.local> Message-ID: <761E2B5697F795489C8710BCC72141FF09E6A1@ex07.net.ucsf.edu> Mehndi, Why? Is your automation costing a lot? Your average is 76 slides/day. That is a substantial number. If your automation is costing a lot, then look for a less costly alternative, like the BioCare or Thermo Fisher (Lab Vision) instruments. Those are completely open systems that can have very low costs if you are careful choosing reagents. We do maybe 50 IF slides per day in our kidney lab and would not get rid of automation. In fact, I would only get rid of automation if we were batching slides only a couple days per week. It saves so much hands on time that only if you have extra staff and very cheap labor costs is it worth dropping. My cost calculations show that even at only 50 slides per day we spend only 10% of our total cost of the test on the Dako (Autostainer Plus, same as Lab Vision 480) automation portion (including service contract costs). The major factor is the cost of reagents. And reagent cost won't change much with manual unless you change all your reagents. But your labor costs will skyrocket. I recommend keeping automation, but evaluate your platform for excess costs. Tim Morken UCSF Pathology -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Histology Sent: Wednesday, October 16, 2013 9:47 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] manual IHC staining Hi all- Does anyone out there still do manual, offline IHC staining? We currently do about 20,000 IHC slides per year and my boss would like to consider dropping the automatic stainers and doing them by hand. If you do manual staining, how long does it take, how much tech time is needed, and what about CAP inspections? Thanks in advance, Mehndi Helgren Dominion Pathology Laboratories 733 Boush St. Suite 200 Norfolk, VA 23510 757-664-7901 histo@pathlab.us _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWatson <@t> gnf.org Wed Oct 16 12:58:39 2013 From: JWatson <@t> gnf.org (James Watson) Date: Wed Oct 16 12:58:44 2013 Subject: [Histonet] Autofluorescence on Retina Tissue In-Reply-To: References: Message-ID: Not sure if this will help with your retinas but we use the copper sulfate treatment on all our fluorescent stains and it eliminates autofluorescence in the RBC completely and helps with other types of autofluorescence. The treatment to be use depends on the cause of the autofluorescence. We counter stain our slides with DAPI. Here are all of our present autofluorescent treatments. Autofluorescence Quenching 1) The best ways to address the issue of autofluorescence in order of preference: a) Avoid it i) Not always possible. b) Try to filter it out during image acquisition i) Difficult due to the broad emission spectrum. c) Chemically remove it i) Can also reduce "real" signal. 2) 10 mM Copper Sulfate a) This treatment is primarily for inhibition of autofluorescence in Red Blood cells, but helps decrease autofluorescence in connective tissue. b) 10 mM Copper Sulfate i) Cupric Sulfate...............1.25 gm. ii) 50 mM Ammonium acetate (pH5)........500.0 ml iii) Adjust pH to 5.0 with 1.0 M NaOH c) 50 mM Ammonium acetate (pH5) i) Ammonium acetate.............1.93 gm. ii) Distilled water................500.0 ml iii) Adjust pH to 5.0 with 1.0 M HCl D) Treatment Procedure i) Rinse in PBS 2 times for 10 minutes each. ii) Rinse in distilled water 5 minutes. iii) Place slides in 10mM copper sulfate for 8 minutes. iv) Return slides to distilled water and check for autofluorescence with microscope. v) If needed return slides to 10mM copper sulfate for a couple of more minutes and check again. vi) Rinse slides for 5 minutes in distilled water. vii) Counterstain with DAPI 5 minutes. viii) Rinse in PBS 2 times for 10 minutes each. ix) Coverslip slides with appropriate mounting media. 3) 2.0 mM Glycine a) Used primarily for autofluorescence caused by free aldehyde groups. b) 2.0 mM Glycine i) Glycine...................3.9 gm. ii) Distilled Water...............26.0 ml c) Treatment Procedure i) Deparaffinize and rehydrate slides to H2O ii) Rinse in PBS 2 times for 10 minutes each. iii) Rinse in distilled water 5 minutes. iv) Place slides in 2.0 mM Glycine for 20-60 minutes. v) Rinse slides for 5 minutes in distilled water. vi) Rinse in PBS 2 times for 10 minutes each. vii) Continue with normal staining procedure 4) Sodium Borohydride a) The use of this reagent is particularly suited to reduce the reversible Schiff's bases that are formed by the aldehyde-NH2 reaction and lead to autofluorescence, especially when using glutaraldehyde. If you can use paraformaldehyde for fixation, the reduction step is often unnecessary and autofluorescence is low. This material has a high potential for explosion and is very caustic. b) The protocol was prepared by Jennifer Kramer and a similar procedure is described by Beisker, et al. (Beisker et al. 1987). i) Immediately before use (1) Make up a 1 mg/ml solution of sodium borohydride in a physiological buffer such as PBS. (a) The solution will be fizzy like carbonated water. Preparing this solution on ice and performing all subsequent incubations on ice has also been recommended. ii) Apply this solution immediately (while fizzing) to cells or tissue sections. (1) For glutaraldehyde fixed cell monolayers incubate in the sodium borohydride solution for 4 minutes. Replace with fresh sodium borohydride solution for another 4 minutes. (2) For paraformaldehyde fixed paraffin embedded 7 ?m sections incubate 3 times, 10 minutes each in sodium borohydride solution. 5) Sudan Black a) This treatment is primarily for inhibition of autofluorescence in Lipfuscin. b) 0.3% Sudan Black (w/v) in 70% EtOH (v/v) stirred in the dark for 2 hours c) Apply to slide for 10 minutes after the secondary antibody application. d) Rinse quickly with PBS 8 times and mount e) For FITC and Alexa 594 this does not reduce the emission signal noticeably James Watson HT? ASCP GNF? Genomics Institute of the Novartis Research Foundation Tel??? 858-332-4647 Fax?? 858-812-1915 jwatson@gnf.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Allyse Mazzarelli Sent: Tuesday, October 15, 2013 11:44 AM To: Histonet Subject: [Histonet] Autofluorescence on Retina Tissue Hi all! Question for all you that may be more immuno-experienced than I: I've consistently run immunofluorescence on pig retina and I seem to have a severe case of autofluorescence/background. I've played around with primary and secondary antibody ratios, but that doesn't seem to help my case. The primary antibodies I use are goat-anti-FLT.1, and mouse-anti-rhodopsin. The secondary antibodies I use are AlexaFluor donkey-anti-goat488 & rabbit-anti-mouse555 (For my experiments, each slide contained only one primary antibody, and it's corresponding secondary. For imaging purposes, I did not double-label on these slides. E.g. FLT.1 was labeled with the 488 secondary, and rhodopsin was labeled with the 555 secondary).I re-hydrate, conduct antigen retrieval, and block as per normal IHC protocol. However, when imaging, I noticed that both slides, labeled with either rhodopsin or FLT.1 seem to "bleed" through to the next filter. For example, mouse-anti-rhodopsin labeled with the 555 secondary works beautifully at a 1:600 ratio. However, when I switch to the FITC filter on my scope, all the retinal tissue appears green on the slides, even though it has ONLY the 555 secondary and NO 488. I've noticed this for the FLT.1 antibody as well (i.e. switch to red filter and tissue fluoresces even though no slide saw the 555 secondary antibody). As I mentioned, I decreased the ratios of all antibodies, but that still doesn't eliminate the problem. If anyone has any ideas as to how I go about fixing this, please let me know. I've only been in the field for a very short period of time, so if I missed something in my description, don't hesitate to ask! Thanks for whatever help you can direct my way! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From melissa <@t> alliedsearchpartners.com Wed Oct 16 13:22:20 2013 From: melissa <@t> alliedsearchpartners.com (Melissa Phelan) Date: Wed Oct 16 13:22:31 2013 Subject: [Histonet] North FL Histology Supervisor Job & South FL Histology Supervisor Job Message-ID: Hello, I currently have two available permanent positions open for a Histology Supervisor. One is in North Florida and one is in South Florida. Please let me know if you would like more details. Thank you, To view a complete list of Allied Search Partners current openings go to: http://www.alliedsearchpartners.com/careers.php -- Melissa Phelan President, Laboratory Staffing Allied Search Partners www.linkedin.com/in/melissaphelan/ http://www.alliedsearchpartners.com T: 888.388.7571 ext. 102 F: 888.388.7572 *If you wish to no longer receive emails from Allied Search Partners please respond to this email message with "remove." This email including its attachments is intended only for the confidential use of the individual to whom it is addressed. If you are not the intended recipient, any use, dissemination, distribution or copying of this message or its attachments is prohibited. If you have received this message in error, please notify us immediately, and delete this message and its attachments permanently from your system. From amosbrooks <@t> gmail.com Wed Oct 16 15:31:42 2013 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Wed Oct 16 15:31:46 2013 Subject: [Histonet] Manual IHC In-Reply-To: References: Message-ID: Hi, I have been doing manual IHC for overflow of what doesn't fit in the Autostainer or for when I do not have enough to really justify running it (10 slides or so). Recently, I have been doing it exclusively since my Autostainer went belly up. Given the numbers you provided I assume you are doing about 75-80 slides per day. While I think this is entirely possible to do, you will find yourself dedicating pretty much the whole day to running these sliked and not having much time for anything else. Advantages: More direct control over each slide, More control over reagent volumes etc. Equipment costs. Disadvantages: It's really easy to get things mixed up and put the wrong reagent on the wrong slide unless you have a really good OCD eye to detail. Time variability (how long to rinse the first to last slide). Some tips if you do this: the Shandon Sequenza is great for applying reagents without fussing over keeping everything level. Rinse the slides *really* well. Be careful with the DAB since you don't need to worry much about it when using an instrument. My personal opinion on this is that given the number of slides you seem to be running an instrument is certainly justified. Best of luck, Amos Brooks From Haley.Huggins <@t> DignityHealth.org Wed Oct 16 15:52:53 2013 From: Haley.Huggins <@t> DignityHealth.org (Huggins, Haley - MRMC) Date: Wed Oct 16 15:53:01 2013 Subject: [Histonet] Cytology Procedure Message-ID: <4F36EC93A5737D4F8A2974E8FB8E260615F57315FF@PHX-MSG-007-N2.chw.edu> Hello all, I am looking for some assistance in Cytology procedures, specifically for non-gyn cytology. I am not as versed in Cytology as I am in Histology, so I am looking for some help with any procedures I might need. I know this is histonet, but I am sure a number of you have had to do cytology from time to time. Thanks in advance for any help. Haley Huggins, HT(ASCP)cm Pathology/Histology Manager Marian Medical Center 1400 East Church St Santa Maria, CA 93454 805-739-3170 (path lab) 805-739-3153 (office) 303-652-7453 (cell) 805-332-8697 (rightfax) From billodonnell <@t> catholichealth.net Wed Oct 16 15:59:28 2013 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Wed Oct 16 15:59:53 2013 Subject: [Histonet] RE: Tissue marking dyes In-Reply-To: References: Message-ID: Thank you all so much for your help and suggestions. I want to apologize for singling out a single company. It turns out, upon further examination, that we have inks from at least three different companies. TBS is actually the least problematic of the whole bunch. I won't mention who makes the others. Bill -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Wednesday, October 16, 2013 10:51 AM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Tissue marking dyes I am wanting to 'cut' the dense tissue dye(s) by TBS (probably same as Cancer Diagnostics or any other). Orange is very thick, so is green. I'm being a bit lazy here, but I was wondering if anyone else is going this? Do you use water, alcohol or H2O2? Any direction would be helpful. Thanks - Bill William (Bill) O'Donnell, HT (ASCP) QIHC Senior Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 SERENITY is not freedom from the storm, but peace amid the storm. Cultivate it in PRAYER! This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From McKenzie.Emily <@t> mhsil.com Wed Oct 16 19:31:20 2013 From: McKenzie.Emily <@t> mhsil.com (McKenzie, Emily) Date: Wed Oct 16 19:31:30 2013 Subject: [Histonet] Desperately seeking information!!! Message-ID: Hello all, A few weeks ago I sent out an information seeking email regarding IHC turnaround time. I did not get much in the way of responses. I figured there was not enough information provided to answer the general questions I was asking. I am having trouble obtaining an national average for IHC turnaround time. I am wondering if all you fellow histoneters out there would be willing to give me some info so I can see were we stand in comparison to facilities of similar size. The facility I work at turns out anywhere from 90-150 IHC stained slides daily. We have an average of 160 cases with around 700 H&E stained slides daily. I have listed a few questions below, if any of you would be so kind as to take the time to answer them it would be greatly appreciated. What is the rough estimate of cases and initial H&E stained slides that are turned out daily? Roughly, how many IHC stained slides do you turn out in a day? On average, what is your IHC turnaround time? What tissues are you working with (general surgical, dermatology's, research etc)? How many techs do you have that can perform IHC staining? Who is your instrumentation through? At the end of the day/run, is there a stain log printed? If so, who signs off on the positive/negative? If there are any other processes/procedures you feel are imperative to your IHC turnaround time please feel free to comment or offer suggestions. Thank you for taking the time to help us to improve our processes. If you have any questions or concerns please let me know. Again, thank you for your help, Emily K. McKenzie BS, HT(ASCP) Memorial Medical Center?701 North First Street?Springfield, IL 62781 Ph: 217-788-3991?email: McKenzie.Emily@mhsil.com ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From slakmon <@t> yahoo.com Wed Oct 16 21:54:45 2013 From: slakmon <@t> yahoo.com (Philip Slakmon) Date: Wed Oct 16 21:54:49 2013 Subject: [Histonet] Diamond Knives Message-ID: <1381978485.36940.YahooMailNeo@web141202.mail.bf1.yahoo.com> Good Evening, I would appreciate getting feedback on what you think of the different brands of Diamond Knives, pro's and con's quality, service, delivery, warranty, price, ... Thank you, Philip From lblazek <@t> digestivespecialists.com Thu Oct 17 07:50:04 2013 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Thu Oct 17 07:50:10 2013 Subject: [Histonet] job opening Dayton Oh area Message-ID: <5A2BD13465E061429D6455C8D6B40E39166713D6F5@IBMB7Exchange.digestivespecialists.com> Come join our team! We have an opening for a full time tech to perform all routine tissue processing embedding, cutting and staining, perform a minimum number of special stains and re-cuts, perform immuno stains on an automated immuno stainer, also perform histology lab maintenance to include maintenance of instrumentation, assist in compliance with CLIA regulations, providing completed preventative maintenance and temperature log sheets for Histology lab. Email me for additional information or send your resume if you're interested in joining our great team! Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Digestive Specialists, Inc Phone: (937) 396-2623 Email: lblazek@digestivespecialists.com From PAMarcum <@t> uams.edu Thu Oct 17 08:17:04 2013 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Thu Oct 17 08:17:10 2013 Subject: [Histonet] Diamond Knives In-Reply-To: <1381978485.36940.YahooMailNeo@web141202.mail.bf1.yahoo.com> References: <1381978485.36940.YahooMailNeo@web141202.mail.bf1.yahoo.com> Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA32D315D6EE@Mail2Node2.ad.uams.edu> Delaware Diamond Knife has always been an excellent company to deal with on diamond knives and tungsten carbide. Pam Marcum -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Philip Slakmon Sent: Wednesday, October 16, 2013 9:55 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Diamond Knives Good Evening, I would appreciate getting feedback on what you think of the different brands of Diamond Knives, pro's and con's quality, service, delivery, warranty, price, ... Thank you, Philip _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From kim.tournear <@t> yahoo.com Thu Oct 17 08:39:02 2013 From: kim.tournear <@t> yahoo.com (Kim Tournear) Date: Thu Oct 17 08:41:41 2013 Subject: [Histonet] free equipment Message-ID: <1382017142.12900.YahooMailNeo@web162602.mail.bf1.yahoo.com> Hi, I work in a derm lab in Tucson AZ. I have a Shandon PathCenter and a Leica Coverslipper (CV5000) I'm giving away. They both need repairs. Free to anyone who wants to pay for the shipping. ? You can respond back at this address for addn'l info. ~Kim~? OU ROCKS!!!! ~Don't be afraid your life will end, be afraid it will never begin~ From barbara.tibbs <@t> accuratediagnosticlabs.com Thu Oct 17 09:01:55 2013 From: barbara.tibbs <@t> accuratediagnosticlabs.com (Barbara Tibbs) Date: Thu Oct 17 09:02:16 2013 Subject: [Histonet] RE: Cytology Procedure In-Reply-To: <4F36EC93A5737D4F8A2974E8FB8E260615F57315FF@PHX-MSG-007-N2.chw.edu> References: <4F36EC93A5737D4F8A2974E8FB8E260615F57315FF@PHX-MSG-007-N2.chw.edu> Message-ID: <3f6a91c719db41e0a33e6b5eb1f94d13@BL2PR04MB196.namprd04.prod.outlook.com> Hi Haley, I've done cytoprep on and off for the past few decades. What exactly do you want to know about non-gyn prep? The first step would be to ask the pathologist what he/she would like prepared i.e. cell block, slides, stains. It's very important to keep the pathologist in the loop when setting all this up. If you could supply more information I could give you some ideas of what I have done in the past. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.tibbs@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Huggins, Haley - MRMC Sent: Wednesday, October 16, 2013 6:52 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Cytology Procedure Hello all, I am looking for some assistance in Cytology procedures, specifically for non-gyn cytology. I am not as versed in Cytology as I am in Histology, so I am looking for some help with any procedures I might need. I know this is histonet, but I am sure a number of you have had to do cytology from time to time. Thanks in advance for any help. Haley Huggins, HT(ASCP)cm Pathology/Histology Manager Marian Medical Center 1400 East Church St Santa Maria, CA 93454 805-739-3170 (path lab) 805-739-3153 (office) 303-652-7453 (cell) 805-332-8697 (rightfax) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From zerfasp <@t> ors.od.nih.gov Thu Oct 17 12:07:16 2013 From: zerfasp <@t> ors.od.nih.gov (Zerfas, Patricia (NIH/OD/ORS) [E]) Date: Thu Oct 17 12:08:48 2013 Subject: [Histonet] maintenance agreement for autostainer Message-ID: <997D36BD8D0ACF418AF900F4E781DE440287F755@MLBXv03.nih.gov> Do you or do you know someone that offers a service maintenance agreement for a 10+ year old DAKO autostainer? Thanks, Patricia M. Zerfas Building 28A, Room 112, MSC 5230 9000 Rockville Pike Bethesda, MD USA 301-496-4464 Fax 301-402-1068 From hans <@t> histologistics.com Thu Oct 17 12:19:43 2013 From: hans <@t> histologistics.com (Hans B Snyder) Date: Thu Oct 17 12:19:48 2013 Subject: [Histonet] Used cytology stainer Message-ID: Hello, We are in need of a super small slide stainer, anything in working condition that is programable. Does anyone have an extra stainer they would like to get rid of? Thank you Hans B Snyder Histologistics 60 Prescott Street Worcester, MA 01605 508-308-7800 hans@histologistics.com From rjr6 <@t> psu.edu Thu Oct 17 12:24:56 2013 From: rjr6 <@t> psu.edu (Roberta Horner) Date: Thu Oct 17 12:25:18 2013 Subject: [Histonet] RE: maintenance agreement for autostainer In-Reply-To: <997D36BD8D0ACF418AF900F4E781DE440287F755@MLBXv03.nih.gov> References: <997D36BD8D0ACF418AF900F4E781DE440287F755@MLBXv03.nih.gov> Message-ID: Check with Dolbey-Jamison. They are out of Philadelphia. 800-220-3073 Roberta Horner HT/HTL Animal Diagnostic Lab Penn State University -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Zerfas, Patricia (NIH/OD/ORS) [E] Sent: Thursday, October 17, 2013 1:07 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] maintenance agreement for autostainer Do you or do you know someone that offers a service maintenance agreement for a 10+ year old DAKO autostainer? Thanks, Patricia M. Zerfas Building 28A, Room 112, MSC 5230 9000 Rockville Pike Bethesda, MD USA 301-496-4464 Fax 301-402-1068 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From elizabeth.heimrich <@t> bms.com Thu Oct 17 13:07:21 2013 From: elizabeth.heimrich <@t> bms.com (Heimrich, Elizabeth) Date: Thu Oct 17 13:07:33 2013 Subject: [Histonet] HT HistoDeck question frozen section fixation Message-ID: <26912ED7286DEB4F8BF000D658C67307048974AC29@ushpwbmsmmp008.one.ads.bms.com> Hi all, Since the key to the question lies in Optimal Fixation, the answer to this question is A, and it is taken right out of the Histotechnology self-instructional text 2nd edition pg 98. (See note) Under Frozen Sections I've included a procedure I have used successfully in the past. It differs from the text book by the addition of the acid hematin pigment removal steps 1. Cut the frozen section and fix in 37%-40% FORMALDEHYDE for 20 sec 2. Cut frozen section and place slides in 37% Formaldehyde (unbuffered) for 30sec -1min (have kept them in formaldehyde longer~20min with no adverse events 3. Treat for acid hematin pigment with saturated alcoholic picric acid (Polyscientific R&D cat# S2357) for 20-30mins 4. Rinse in 95% EtOH 2x 5. 70% EtOH 1x 6. Running tap H20 until all yellow pigment has been removed 7. Rinse in dH2O and stain with H&E protocol Carson's NOTE: Many labs use acetone or alcohol for frozen section fixation; however Conc. Formaldehyde yields morphologic preservation as seen in permanent sections. Hope this helps. Beth ________________________________ This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. From Elizabeth.Cameron <@t> jax.org Thu Oct 17 13:41:32 2013 From: Elizabeth.Cameron <@t> jax.org (Elizabeth Cameron) Date: Thu Oct 17 13:41:38 2013 Subject: [Histonet] Autosection? Message-ID: Has anyone out there tried the Tissue Tek Autosection? I would love to get some feedback on it. Thanks. The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as possible. From SHUNTER <@t> beaumont.edu Thu Oct 17 15:30:25 2013 From: SHUNTER <@t> beaumont.edu (Sue Hunter) Date: Thu Oct 17 15:30:41 2013 Subject: [Histonet] RE: maintenance agreement for autostainer In-Reply-To: <997D36BD8D0ACF418AF900F4E781DE440287F755@MLBXv03.nih.gov> References: <997D36BD8D0ACF418AF900F4E781DE440287F755@MLBXv03.nih.gov> Message-ID: Try Victor Wong, Autostainer Technical Solutions 888-505-9545 cell 646-378-9222 www.autostainertech.com Not sure if he is still in business, he was located in New Windsor NY Sue Sue Hunter, Supervisor Advanced Diagnostics Beaumont Health System Royal Oak MI 248-898-5146 shunter@beaumont.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Zerfas, Patricia (NIH/OD/ORS) [E] Sent: Thursday, October 17, 2013 1:07 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] maintenance agreement for autostainer Do you or do you know someone that offers a service maintenance agreement for a 10+ year old DAKO autostainer? Thanks, Patricia M. Zerfas Building 28A, Room 112, MSC 5230 9000 Rockville Pike Bethesda, MD USA 301-496-4464 Fax 301-402-1068 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kim.tournear <@t> yahoo.com Thu Oct 17 17:31:47 2013 From: kim.tournear <@t> yahoo.com (Kim Tournear) Date: Thu Oct 17 17:31:54 2013 Subject: [Histonet] Re: free equipment. Message-ID: <4782635D-B6A4-4DF4-A5E9-1EA8E6415F56@yahoo.com> The pathcenter needs a rotor and the coverslipper needs a motherboard. Sent from the iPhone of Kim Tournear ?? ? From Nancy_Schmitt <@t> pa-ucl.com Fri Oct 18 09:04:42 2013 From: Nancy_Schmitt <@t> pa-ucl.com (Nancy Schmitt) Date: Fri Oct 18 09:04:49 2013 Subject: [Histonet] Pathology Assistant Message-ID: <906B4DA90ED1DB4DB6C7E94D7CEE6C368150AB04@PEITHA.wad.pa-ucl.com> Good Morning- Regulations state that when you have a PA their grossing needs to be reviewed within 24 hours. How is everyone handling this for Friday workload? Thank you for your input- Nancy Nancy Schmitt HT, MLT(ASCP) Histology Coordinator United Clinical Laboratories NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From relia1 <@t> earthlink.net Fri Oct 18 09:24:06 2013 From: relia1 <@t> earthlink.net (Pam Barker) Date: Fri Oct 18 09:24:18 2013 Subject: [Histonet] RELIA HOT HISTOLOGY JOB ALERT!!!! LEADING Edge Lab in Louisville, KY Histotechnologist needed IHC FISH ISH !!!!!! TGIF!!!! Message-ID: <001801cecc0d$b4b6e7d0$1e24b770$@earthlink.net> Hi Histonetters!! TGIF!!! I hope everyone is ending their week on a high note and getting ready to enjoy another lovely Fall weekend. Before you head off I want to tell you about a position I am pretty excited about. My company RELIA has been engaged by a leading edge laboratory to assist them in their search for a histotechnologist for their growing staff in Louisville, KY. ASCP HT/HTL and 2-3 years of histology experience along with IHC is required. FISH ISH and grossing of small specimens is preferred. My client offers excellent compensation, benefits, relocation assistance and the opportunity to train in and work on the forefront of molecular diagnostics and cytogenetics. If you would like more details please give me a call at 866-607-3542 or shoot me an email at relia1@earthlink.net Have a great weekend!! Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From ncosenza <@t> siumed.edu Sat Oct 19 09:50:32 2013 From: ncosenza <@t> siumed.edu (ncosenza@siumed.edu) Date: Sat Oct 19 09:50:35 2013 Subject: [Histonet] fixation question Message-ID: <20131019095032.hm9jsk4grusks8gc@webmail.siumed.edu> Hello Histonetters: Our lab has a resident wanting to start a project involving drug effect on tumor size. Since he is wanting to compare tumor size at certain time points after drug treatment (and do IHC staining intensity), he wonders how much affect varying fixation times will have on the tissue. We are not doing the processing or embedding ourselves so we can't control for such variation. Is this a legitimate concern? If one piece of tissue sits in fix 24 hours before processing/embedding, will the morphology be drastically different than a second piece that sits longer (or shorter) than 24 hours? Is so, would fixing the tissue ourselves for a set time and then putting in some sort of buffer or ethanol before we ship the tissue to the company who embeds it make a difference? The resident is really trying to minimize swelling or excess shrinkage of the tissue. Thanks! From chesarato <@t> hotmail.com Sun Oct 20 12:35:26 2013 From: chesarato <@t> hotmail.com (Cesar Francisco Romero) Date: Sun Oct 20 12:35:31 2013 Subject: [Histonet] RE: Fixation Question Message-ID: The total Osmolarity of the fixing solution should be isosmotic with tissue fluids in order to prevent shrinkage or swelling of cells. The best way to avoid shrinkage is to prepare Formalin with Physiological or Isotonic Saline Solution. The solution is 9 grams of sodium chloride ( NaCl ) dissolved in Distilled Water, to a total volume of 1000 ml. To prepare the fixative use 100 ml of pure Formalin ( Formalin 37 ~ 40 % ) and 900 ml of Isotonic Saline Solution. Cesar Romero Buenos Aires Argentina From chesarato <@t> hotmail.com Sun Oct 20 13:26:21 2013 From: chesarato <@t> hotmail.com (Cesar Francisco Romero) Date: Sun Oct 20 13:26:33 2013 Subject: [Histonet] Re: Fixation Question Message-ID: I forgot to say that the rest of the tissue processing can cause shrinkage. If the paraffin is too hot you will get fried tissues. Here is a Link with very good information about tissue processing. http://users.adam.com.au/royellis/tp/tp.htm From JMacDonald <@t> mtsac.edu Mon Oct 21 01:32:07 2013 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Mon Oct 21 01:32:14 2013 Subject: [Histonet] California Society for Histotechnology Meeting Message-ID: The California Society for Histotechnology annual symposium is scheduled for May 2-4, 2014 at the Hilton Woodland Hills (southern CA, LA area). We are now accepting abstracts. If you are interested in speaking please contact me and I will send you an application. Thank you, Jennifer MacDonald CSH Secretary jmacdonaldcsh@gmail.com jmacdonald@mtsac.edu From gu.lang <@t> gmx.at Mon Oct 21 02:37:32 2013 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Mon Oct 21 02:38:10 2013 Subject: AW: [Histonet] fixation question In-Reply-To: <20131019095032.hm9jsk4grusks8gc@webmail.siumed.edu> References: <20131019095032.hm9jsk4grusks8gc@webmail.siumed.edu> Message-ID: <000001cece30$67a37240$36ea56c0$@gmx.at> Hi! In my opinion 24 hours should be the minimal duration for fixation in 4% buffered formaldehyde. The shorter the time the more differences may occur. It is important to prepare the specimens in the same way at the grossing - means same size of tissue in the cassette or at least minimal size of 3 mm diameter. 24 hours of a whole organ in formalin is not the same as 24 hours of a small tissue-block. Look at the article of Cecil Fox about formaldehyde. He says that shrinkage is not due to formalin, but more caused by the processing, when lipids and water are removed. The better the crosslinking, the less sensitive is the tissue to shrinkage or swelling. Immunhisto can be adopted to longer fixation, but too short fixation may give impact on the morphology, that can't be recovered. Shrinkage during processing can be intensified by very long times in absolute ethanol and xylen. Let you inform about the processing protocol of the company. The protocol should fit to your specimens. On the other side inform the company about your fixation times. They may prolong the antigen retrieval for better results. My advice without knowledge of your special experiment is a fixationtime of 24-72 hours. Bye Gudrun Lang -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von ncosenza@siumed.edu Gesendet: Samstag, 19. Oktober 2013 16:51 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] fixation question Hello Histonetters: Our lab has a resident wanting to start a project involving drug effect on tumor size. Since he is wanting to compare tumor size at certain time points after drug treatment (and do IHC staining intensity), he wonders how much affect varying fixation times will have on the tissue. We are not doing the processing or embedding ourselves so we can't control for such variation. Is this a legitimate concern? If one piece of tissue sits in fix 24 hours before processing/embedding, will the morphology be drastically different than a second piece that sits longer (or shorter) than 24 hours? Is so, would fixing the tissue ourselves for a set time and then putting in some sort of buffer or ethanol before we ship the tissue to the company who embeds it make a difference? The resident is really trying to minimize swelling or excess shrinkage of the tissue. Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ree3 <@t> leicester.ac.uk Mon Oct 21 02:50:47 2013 From: ree3 <@t> leicester.ac.uk (Edwards, Richard E.) Date: Mon Oct 21 02:51:03 2013 Subject: [Histonet] another fixation question Message-ID: <7722595275A4DD4FA225B92CDBF174A101B0DAD7145F@EXC-MBX3.cfs.le.ac.uk> Hi All, I am looking into the pros and cons of fixation in acetone(4C) and 10% neutral buffered formalin, my opinion is that NBF wins all the time, I would warmly welcome any further opinions on this topic, many thanks. Richard Edwards University of Leicester U.K. From njoydobro <@t> aol.com Mon Oct 21 10:16:54 2013 From: njoydobro <@t> aol.com (njoydobro@aol.com) Date: Mon Oct 21 10:16:59 2013 Subject: [Histonet] Steamer & PT Module ? Message-ID: <8D09C87B5DCCEDD-73C-2BF87@webmail-vm004.sysops.aol.com> good morning Histonetters, ? ???? Does anyone out there know of a COMMERCIAL grade/approved steamer for antigen retrieval use.? Our lab will not let us use one of traditional Black?& Decker steamers that most use.? It must say for commercial use or be labeled as "Laboratory". ? If not, does anyone use the PT Module from Thermo-Scientifc?? Thoughts? ? Anyone have one of these they wouldl like to sell? ? Thanks, Gene ? From Terra.Wineman <@t> novusint.com Mon Oct 21 10:29:38 2013 From: Terra.Wineman <@t> novusint.com (Wineman, Terra) Date: Mon Oct 21 10:30:02 2013 Subject: [Histonet] Steamer & PT Module ? In-Reply-To: <8D09C87B5DCCEDD-73C-2BF87@webmail-vm004.sysops.aol.com> References: <8D09C87B5DCCEDD-73C-2BF87@webmail-vm004.sysops.aol.com> Message-ID: <1EB8F245A303564EADF12AC7022FA74D70F1B712@NOVUS-EX01.novusint.com> Biocare has a decloaker that we use for HIER that works great. Cell Marque and other major suppliers have various decloaker models. Terra Wineman, HTL (ASCP)CM Research Biologist 636-926-7476 phone terra.wineman@novusint.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of njoydobro@aol.com Sent: Monday, October 21, 2013 10:17 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Steamer & PT Module ? good morning Histonetters, ? ???? Does anyone out there know of a COMMERCIAL grade/approved steamer for antigen retrieval use.? Our lab will not let us use one of traditional Black?& Decker steamers that most use.? It must say for commercial use or be labeled as "Laboratory". ? If not, does anyone use the PT Module from Thermo-Scientifc?? Thoughts? ? Anyone have one of these they wouldl like to sell? ? Thanks, Gene ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wbenton <@t> cua.md Mon Oct 21 10:32:13 2013 From: wbenton <@t> cua.md (Walter Benton) Date: Mon Oct 21 10:32:46 2013 Subject: [Histonet] Steamer & PT Module ? In-Reply-To: <8D09C87B5DCCEDD-73C-2BF87@webmail-vm004.sysops.aol.com> References: <8D09C87B5DCCEDD-73C-2BF87@webmail-vm004.sysops.aol.com> Message-ID: <0B8979A204680A42B93A52B486088CD93931ABEF67@CUAEXH1.GCU-MD.local> Biocare and Dako should have something that meets your needs. Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) ChesapeakeUrology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of njoydobro@aol.com [njoydobro@aol.com] Sent: Monday, October 21, 2013 11:16 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Steamer & PT Module ? good morning Histonetters, ? ???? Does anyone out there know of a COMMERCIAL grade/approved steamer for antigen retrieval use.? Our lab will not let us use one of traditional Black?& Decker steamers that most use.? It must say for commercial use or be labeled as "Laboratory". ? If not, does anyone use the PT Module from Thermo-Scientifc?? Thoughts? ? Anyone have one of these they wouldl like to sell? ? Thanks, Gene ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From njoydobro <@t> aol.com Mon Oct 21 10:34:50 2013 From: njoydobro <@t> aol.com (njoydobro@aol.com) Date: Mon Oct 21 10:34:54 2013 Subject: [Histonet] Steamer & PT Module ? In-Reply-To: <0B8979A204680A42B93A52B486088CD93931ABEF67@CUAEXH1.GCU-MD.local> References: <0B8979A204680A42B93A52B486088CD93931ABEF67@CUAEXH1.GCU-MD.local> Message-ID: <8D09C8A3764219D-73C-2C2E4@webmail-vm004.sysops.aol.com> We are in of?a STEAMER NOT A PRESSURE COOKER...... ? -----Original Message----- From: Walter Benton <wbenton@cua.md> To: njoydobro <njoydobro@aol.com>; histonet <histonet@lists.utsouthwestern.edu> Sent: Mon, Oct 21, 2013 11:32 am Subject: RE: [Histonet] Steamer & PT Module ? Biocare and Dako should have something that meets your needs. Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) ChesapeakeUrology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of njoydobro@aol.com [njoydobro@aol.com] Sent: Monday, October 21, 2013 11:16 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Steamer & PT Module ? good morning Histonetters, ? ???? Does anyone out there know of a COMMERCIAL grade/approved steamer for antigen retrieval use.? Our lab will not let us use one of traditional Black?& Decker steamers that most use.? It must say for commercial use or be labeled as "Laboratory". ? If not, does anyone use the PT Module from Thermo-Scientifc?? Thoughts? ? Anyone have one of these they wouldl like to sell? ? Thanks, Gene ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From TJohnson <@t> gnf.org Mon Oct 21 10:49:33 2013 From: TJohnson <@t> gnf.org (Teri Johnson) Date: Mon Oct 21 10:49:39 2013 Subject: [Histonet] Re: fixation question Message-ID: <9F3CFEE76E51B64991C7485270890B40497D6A80@EX5.lj.gnf.org> Fixation and processing has a huge effect on tissue size. My advice is to have them do the measurements prior to fixation upon harvest. That makes the fixation issue completely irrelevant. If you want to later validate it, you can control fixation time by using the same time (whatever you determine is appropriate) across multiple specimens and then store them in 70% ethanol to send. The important thing is to standardize everything you can. Teri Johnson Manager, Histology Genomics Institute for Novartis Research Foundation San Diego, CA 858-332-4752 From TJohnson <@t> gnf.org Mon Oct 21 12:17:24 2013 From: TJohnson <@t> gnf.org (Teri Johnson) Date: Mon Oct 21 12:17:33 2013 Subject: [Histonet] Re: fixation question Message-ID: <9F3CFEE76E51B64991C7485270890B40497D6D0C@EX5.lj.gnf.org> Dr. Charles Scouten addressed this issue regarding changes of brain due to different perfusion fixation techniques. He has an image showing the difference between a fresh brain, one pressure perfused with sucrose followed by 4% paraformaldehyde/glutaraldehyde, and the last one with gravity flow perfusion using the same materials. Ref: http://www.leicabiosystems.com/pathologyleaders/sacrifice-perfusion-in-animal-research/ My thought is that if you see shrinkage of the material depending on perfusion fixation methods, you will likely see it with immersion fixation. Teri Johnson Manager, Histology Genomics Institute for Novartis Research Foundation San Diego, CA 858-332-4752 From catherinesimonson <@t> gmail.com Mon Oct 21 13:14:36 2013 From: catherinesimonson <@t> gmail.com (Catherine Simonson) Date: Mon Oct 21 13:14:41 2013 Subject: [Histonet] Leica slide printers Message-ID: I was wondering if anybody out there in Histoland had any experience with the new Leica slide printer? What are your thoughts? Thanks in advance, Catherine From barbara.tibbs <@t> accuratediagnosticlabs.com Mon Oct 21 14:27:24 2013 From: barbara.tibbs <@t> accuratediagnosticlabs.com (Barbara Tibbs) Date: Mon Oct 21 14:27:38 2013 Subject: [Histonet] Leica slide printers In-Reply-To: References: Message-ID: The Leica IP S? Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.tibbs@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Catherine Simonson Sent: Monday, October 21, 2013 4:14 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica slide printers I was wondering if anybody out there in Histoland had any experience with the new Leica slide printer? What are your thoughts? Thanks in advance, Catherine _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From adesupo2002 <@t> hotmail.com Mon Oct 21 22:50:06 2013 From: adesupo2002 <@t> hotmail.com (ADESUPO ADESUYI) Date: Mon Oct 21 22:50:16 2013 Subject: [Histonet] Inadequacy of biopsy Message-ID: Hi, I am wondering how you guys in histo land treats biopsy that is inadequate for evaluation. If there is a policy that addresses this issue that you guys will like to share with me, I will really appreciate it. Lately we have been receiving biopsies that are not big enough for evaluation and this has been causing a lot of problems. Thanking you guys for your anticipated cooperation. Banjo Adesuyi From dmccaig <@t> ckha.on.ca Tue Oct 22 06:32:28 2013 From: dmccaig <@t> ckha.on.ca (Diana McCaig) Date: Tue Oct 22 06:32:45 2013 Subject: [Histonet] Endometrial biopsies Message-ID: In the past when we receive these samples there has been no issues cutting them. Over the past few months there has been a tendency for these blocks to shred into fine strips when cut. Almost like there was sand in the blocks. Mollifex, decal, warm or cold, doesn't matter they still shred. Getting levels is such a challenge. I have inquired at the collection site to determine if there is a change in the process of collecting that could cause this. I have ensured the samples are not left on gauze or something to allow them to dry out. Even blocks with lots of blood and/or mucous will shred as well as blocks with only a scanty amount present. Is anyone else experiencing this. Diana Chatham Kent Health Alliance From lcolbert <@t> pathmdlabs.com Tue Oct 22 08:04:36 2013 From: lcolbert <@t> pathmdlabs.com (Laurie Colbert) Date: Tue Oct 22 08:08:47 2013 Subject: [Histonet] RE: Endometrial biopsies In-Reply-To: References: Message-ID: <12ECD7346266D74691EC2BFC75285E452F361E27@BFL323E10.pathmdlabs.local> Diana, This has always been an issue for us. We wrap currettings in lens paper, and my best guess is that the embedders scrape the paper to get the tissue off and scrape up small amounts of the lens paper, which then shreds. I tell them to be careful and try not to scrape too hard, but it doesn't always help. Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Diana McCaig Sent: Tuesday, October 22, 2013 4:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Endometrial biopsies In the past when we receive these samples there has been no issues cutting them. Over the past few months there has been a tendency for these blocks to shred into fine strips when cut. Almost like there was sand in the blocks. Mollifex, decal, warm or cold, doesn't matter they still shred. Getting levels is such a challenge. I have inquired at the collection site to determine if there is a change in the process of collecting that could cause this. I have ensured the samples are not left on gauze or something to allow them to dry out. Even blocks with lots of blood and/or mucous will shred as well as blocks with only a scanty amount present. Is anyone else experiencing this. Diana Chatham Kent Health Alliance _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mhale <@t> MiracaLS.com Tue Oct 22 09:23:55 2013 From: mhale <@t> MiracaLS.com (Hale, Meredith) Date: Tue Oct 22 09:24:05 2013 Subject: [Histonet] CLIA Message-ID: <0E828EC51C7CC445A51E53F81B64E8C737A2D2@s-irv-exchmb.PathologyPartners.intranet> Has anyone out there been told by CLIA you must run a negative control along with a positive control for HP special stains , not IHC ? I know we do this for AFB but never have for an HP special stain . Also the new IQCP CLIA is rolling out it states that if your lab doesn't enroll in the voluntary IQCP that you must perform tow external controls on each test system for each day . Is this what they may be referring to ? Thanks in advance . Meredith Hale HT (ASCP)cm Director External Sales Support Miraca Life Sciences 6655 North MacArthur Blvd. Irving , Texas 75039 Office: 214-596-2219 Cell: 469-648-8253 Fax: 1-866-688-3280 mhale@miracals.com> From jerrysedgewick <@t> gmail.com Tue Oct 22 09:42:11 2013 From: jerrysedgewick <@t> gmail.com (jerry sedgewick) Date: Tue Oct 22 09:42:15 2013 Subject: [Histonet] golgi stain In-Reply-To: <12ECD7346266D74691EC2BFC75285E452F361E27@BFL323E10.pathmdlabs.local> References: <12ECD7346266D74691EC2BFC75285E452F361E27@BFL323E10.pathmdlabs.local> Message-ID: <52668EC3.304@gmail.com> I am looking for 2 slides that have tissue stained with a golgi stain. I'm working with a company that is interested in creating an imaging product, and they are interested in testing with a golgi stain. Unfortunately, I can't find such a slide at Triarch or Carolina Biological Supply. I wondered if anyone would know where I could get these slides. Jerry Sedgewick jerry@imagingandanalysis.com From shawnleslie <@t> embarqmail.com Tue Oct 22 10:07:01 2013 From: shawnleslie <@t> embarqmail.com (Shawn Leslie ) Date: Tue Oct 22 10:07:05 2013 Subject: [Histonet] Low Temp question Message-ID: <1213515045.960380.1382454421024.JavaMail.root@embarqmail.com> Goodmorning all, ????I have a question concerning the Ventana Benchmark XT. We are constantly having low temperature errors during almost every run. The immunos look fine but we are still concerned. Has anyone else that uses the Benchmark experienced this also ? We have noticed that the silver coating on the heating pads is wearing off. Ventana indicated to us that it's nothing to be concerned about. Any hel p would be appreciated. Shawn Leslie HT (ASCP) From flnails <@t> texaschildrens.org Tue Oct 22 10:09:57 2013 From: flnails <@t> texaschildrens.org (Nails, Felton) Date: Tue Oct 22 10:10:02 2013 Subject: [Histonet] Leica slide printers In-Reply-To: References: Message-ID: <327E034F1892504289B7A17EC71DF9F304AF01@TCFMSG03.ad.texaschildrenshospital.org> If you are referring to the IPS slide writer, I would say continue looking it is very unreliable. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Catherine Simonson Sent: Monday, October 21, 2013 1:15 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica slide printers I was wondering if anybody out there in Histoland had any experience with the new Leica slide printer? What are your thoughts? Thanks in advance, Catherine _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ______________________________________________________________________ From barbara.tibbs <@t> accuratediagnosticlabs.com Tue Oct 22 10:21:07 2013 From: barbara.tibbs <@t> accuratediagnosticlabs.com (Barbara Tibbs) Date: Tue Oct 22 10:21:26 2013 Subject: [Histonet] Leica slide printers In-Reply-To: <327E034F1892504289B7A17EC71DF9F304AF01@TCFMSG03.ad.texaschildrenshospital.org> References: , <327E034F1892504289B7A17EC71DF9F304AF01@TCFMSG03.ad.texaschildrenshospital.org> Message-ID: <70eb78f0d6d740999241d7a4a66338fb@BL2PR04MB196.namprd04.prod.outlook.com> I have had no problem with the Leica IPS. I'm fastidious about maintenance so perhaps that's the secret to my success. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.tibbs@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Nails, Felton Sent: Tuesday, October 22, 2013 1:09 PM To: 'Catherine Simonson'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Leica slide printers If you are referring to the IPS slide writer, I would say continue looking it is very unreliable. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Catherine Simonson Sent: Monday, October 21, 2013 1:15 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica slide printers I was wondering if anybody out there in Histoland had any experience with the new Leica slide printer? What are your thoughts? Thanks in advance, Catherine _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TMcNemar <@t> lmhealth.org Tue Oct 22 10:27:56 2013 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Tue Oct 22 10:28:05 2013 Subject: [Histonet] Low Temp question In-Reply-To: <1213515045.960380.1382454421024.JavaMail.root@embarqmail.com> References: <1213515045.960380.1382454421024.JavaMail.root@embarqmail.com> Message-ID: We see these continually as well. We are told that it is a software glitch and not really a problem with the heat. I was told that they are working on it. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shawn Leslie Sent: Tuesday, October 22, 2013 11:07 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Low Temp question Goodmorning all, I have a question concerning the Ventana Benchmark XT. We are constantly having low temperature errors during almost every run. The immunos look fine but we are still concerned. Has anyone else that uses the Benchmark experienced this also ? We have noticed that the silver coating on the heating pads is wearing off. Ventana indicated to us that it's nothing to be concerned about. Any hel p would be appreciated. Shawn Leslie HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From flnails <@t> texaschildrens.org Tue Oct 22 10:46:06 2013 From: flnails <@t> texaschildrens.org (Nails, Felton) Date: Tue Oct 22 10:47:11 2013 Subject: [Histonet] Leica slide printers In-Reply-To: <70eb78f0d6d740999241d7a4a66338fb@BL2PR04MB196.namprd04.prod.outlook.com> References: , <327E034F1892504289B7A17EC71DF9F304AF01@TCFMSG03.ad.texaschildrenshospital.org> <70eb78f0d6d740999241d7a4a66338fb@BL2PR04MB196.namprd04.prod.outlook.com> Message-ID: <327E034F1892504289B7A17EC71DF9F304AFE3@TCFMSG03.ad.texaschildrenshospital.org> Maybe, but Leica actually replaced mine within the first year because of all of the problems. -----Original Message----- From: Barbara Tibbs [mailto:barbara.tibbs@accuratediagnosticlabs.com] Sent: Tuesday, October 22, 2013 10:21 AM To: Nails, Felton; 'Catherine Simonson'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Leica slide printers I have had no problem with the Leica IPS. I'm fastidious about maintenance so perhaps that's the secret to my success. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.tibbs@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Nails, Felton Sent: Tuesday, October 22, 2013 1:09 PM To: 'Catherine Simonson'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Leica slide printers If you are referring to the IPS slide writer, I would say continue looking it is very unreliable. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Catherine Simonson Sent: Monday, October 21, 2013 1:15 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica slide printers I was wondering if anybody out there in Histoland had any experience with the new Leica slide printer? What are your thoughts? Thanks in advance, Catherine _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ______________________________________________________________________ From jerrysedgewick <@t> gmail.com Tue Oct 22 11:40:27 2013 From: jerrysedgewick <@t> gmail.com (jerry sedgewick) Date: Tue Oct 22 11:40:33 2013 Subject: [Histonet] golgi stain In-Reply-To: <327E034F1892504289B7A17EC71DF9F304AFE3@TCFMSG03.ad.texaschildrenshospital.org> References: , <327E034F1892504289B7A17EC71DF9F304AF01@TCFMSG03.ad.texaschildrenshospital.org> <70eb78f0d6d740999241d7a4a66338fb@BL2PR04MB196.namprd04.prod.outlook.com> <327E034F1892504289B7A17EC71DF9F304AFE3@TCFMSG03.ad.texaschildrenshospital.org> Message-ID: <5266AA7B.7000707@gmail.com> To be clearer about the particular golgi stain used for microscope slides that I need (ultimately for commercial purposes to develop an imaging product), the kit mentioned below would be appropriate (or something like it): *FD Rapid GolgiStain Kit from FD NeuroTechnologies Inc (Cat # PK401)* Thanks! Jerry Sedgewick jerry@imagingandanalysis.com From SHEILA.HERRINGTON <@t> interiorhealth.ca Tue Oct 22 13:04:45 2013 From: SHEILA.HERRINGTON <@t> interiorhealth.ca (HERRINGTON, SHEILA) Date: Tue Oct 22 13:05:04 2013 Subject: [Histonet] Low Temp question In-Reply-To: References: <1213515045.960380.1382454421024.JavaMail.root@embarqmail.com> Message-ID: <9D5F3F245A5FE0458E46E9A71E3EE08C06CD37F350@DC1SERV352.interiorhealth.ca> I also have been having low temp errors. I use Benchmark Ultras and they are not a batch stainer, so pads are heated independently. I noticed the staining on the particular pad with the error was not quite to the usual standard so I have stopped using that pad. Have contacted Ventana for a case number, but have not got an answer. Is this a new problem for you as well? This only started a few weeks ago for us. Sheila Herrington Technical Lead Histopathology and Immunohistochemistry Kelowna General Hospital 2268 Pandosy Street, Kelowna, B.C. V1Y 1T2 250-862-4300 ext 7587 or 7510 Sheila.herrington@interiorhealth.ca -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Tuesday, October 22, 2013 8:28 AM To: 'Shawn Leslie '; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Low Temp question We see these continually as well. We are told that it is a software glitch and not really a problem with the heat. I was told that they are working on it. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shawn Leslie Sent: Tuesday, October 22, 2013 11:07 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Low Temp question Goodmorning all, I have a question concerning the Ventana Benchmark XT. We are constantly having low temperature errors during almost every run. The immunos look fine but we are still concerned. Has anyone else that uses the Benchmark experienced this also ? We have noticed that the silver coating on the heating pads is wearing off. Ventana indicated to us that it's nothing to be concerned about. Any hel p would be appreciated. Shawn Leslie HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From jshelley <@t> sanfordburnham.org Tue Oct 22 13:16:10 2013 From: jshelley <@t> sanfordburnham.org (John Shelley) Date: Tue Oct 22 13:16:16 2013 Subject: [Histonet] Low Temp question In-Reply-To: <9D5F3F245A5FE0458E46E9A71E3EE08C06CD37F350@DC1SERV352.interiorhealth.ca> References: <1213515045.960380.1382454421024.JavaMail.root@embarqmail.com> <9D5F3F245A5FE0458E46E9A71E3EE08C06CD37F350@DC1SERV352.interiorhealth.ca> Message-ID: I had a whole tray changed out because of this type of problem. The company has said that the way the Benchmark /Discovery XT's were made that liquid is getting under the pad and causing some board issues. My service guy had said that this would not be a problem with the Ultra's but now I wonder. I would press Ventana since you had seen a drop-off in staining this means something is mechanically going wrong with your pad, especially if you have a service contract or it is under warranty. Since the Ultra tray is independent from one another, it is most likely just this pad and tray. Kind Regards! ? John J Shelley Research Specialist, Histology Core Facility Sanford-Burnham Medical Research Institute at Lake Nona 6400 Sanger Road??????????????????????????????? Orlando, FL 32827??????????????????????????????????? Tel: (407) 745-2000 Ext.2517 Lab: (407) 745-2119 Fax: (407) 745-2001 email:? jshelley@sanfordburnham.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of HERRINGTON, SHEILA Sent: Tuesday, October 22, 2013 2:05 PM To: 'Tom McNemar'; 'Shawn Leslie '; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Low Temp question I also have been having low temp errors. I use Benchmark Ultras and they are not a batch stainer, so pads are heated independently. I noticed the staining on the particular pad with the error was not quite to the usual standard so I have stopped using that pad. Have contacted Ventana for a case number, but have not got an answer. Is this a new problem for you as well? This only started a few weeks ago for us. Sheila Herrington Technical Lead Histopathology and Immunohistochemistry Kelowna General Hospital 2268 Pandosy Street, Kelowna, B.C. V1Y 1T2 250-862-4300 ext 7587 or 7510 Sheila.herrington@interiorhealth.ca -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Tuesday, October 22, 2013 8:28 AM To: 'Shawn Leslie '; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Low Temp question We see these continually as well. We are told that it is a software glitch and not really a problem with the heat. I was told that they are working on it. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shawn Leslie Sent: Tuesday, October 22, 2013 11:07 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Low Temp question Goodmorning all, I have a question concerning the Ventana Benchmark XT. We are constantly having low temperature errors during almost every run. The immunos look fine but we are still concerned. Has anyone else that uses the Benchmark experienced this also ? We have noticed that the silver coating on the heating pads is wearing off. Ventana indicated to us that it's nothing to be concerned about. Any hel p would be appreciated. Shawn Leslie HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From jbro22 <@t> lsuhsc.edu Tue Oct 22 14:05:30 2013 From: jbro22 <@t> lsuhsc.edu (Browning, Jeffrey A.) Date: Tue Oct 22 14:05:38 2013 Subject: [Histonet] Electron Microscopy Vacancy Message-ID: <2E3D5719BC42374E982EF0AD611F236292840FE6@SH-ExchMB1.master.lsuhsc.edu> Histonetters, Our Electron Microscopy Lab is seeking a technician for processing, microtomy, and/or microscopy of kidney, muscle, cilia, and other patient specimens for transmission electron microscope. Practical experience is desired; healthcare licensure is not required. Please email me directly for more information. Thanks! Jeff Browning, HTL(ASCP) Technical Director, Anatomic Pathology Department of Pathology LSU Health Science Center - Shreveport 1501 Kings Highway Shreveport, LA 71103 (318) 675-5872 jbro22@lsuhsc.edu From billodonnell <@t> catholichealth.net Tue Oct 22 14:33:53 2013 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Tue Oct 22 14:33:59 2013 Subject: [Histonet] RE: Endometrial biopsies In-Reply-To: <12ECD7346266D74691EC2BFC75285E452F361E27@BFL323E10.pathmdlabs.local> References: <12ECD7346266D74691EC2BFC75285E452F361E27@BFL323E10.pathmdlabs.local> Message-ID: I have found that if using tissue paper wraps for bloody tissues, that if I wet them first with water, the paper does not need to be scraped so often or so violently. - Bill -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Tuesday, October 22, 2013 8:05 AM To: Diana McCaig; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Endometrial biopsies Diana, This has always been an issue for us. We wrap currettings in lens paper, and my best guess is that the embedders scrape the paper to get the tissue off and scrape up small amounts of the lens paper, which then shreds. I tell them to be careful and try not to scrape too hard, but it doesn't always help. Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Diana McCaig Sent: Tuesday, October 22, 2013 4:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Endometrial biopsies In the past when we receive these samples there has been no issues cutting them. Over the past few months there has been a tendency for these blocks to shred into fine strips when cut. Almost like there was sand in the blocks. Mollifex, decal, warm or cold, doesn't matter they still shred. Getting levels is such a challenge. I have inquired at the collection site to determine if there is a change in the process of collecting that could cause this. I have ensured the samples are not left on gauze or something to allow them to dry out. Even blocks with lots of blood and/or mucous will shred as well as blocks with only a scanty amount present. Is anyone else experiencing this. Diana Chatham Kent Health Alliance _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. From sdysart <@t> mirnarx.com Tue Oct 22 15:05:55 2013 From: sdysart <@t> mirnarx.com (Sarah Dysart) Date: Tue Oct 22 15:06:01 2013 Subject: [Histonet] PAXgene Tissue Container Message-ID: <2a099777514648f18556edf4347efb2b@BY2PR07MB106.namprd07.prod.outlook.com> Has anyone ever used this? Apparently it contains two proprietary reagents (a fixative and a transport media). They specifically say it's not formalin (so alcohol or the likes I assume). Just curious if anyone has used it and how it worked for them. Thanks http://www.qiagen.com/products/catalog/sample-technologies/rna-sample-technologies/dna-rna-protein/paxgene-tissue-containers#productdetails Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 From carol.wilson <@t> ricerca.com Tue Oct 22 15:10:10 2013 From: carol.wilson <@t> ricerca.com (Wilson, Carol) Date: Tue Oct 22 15:10:14 2013 Subject: [Histonet] RE: Leica Slide Printers Message-ID: <19848F1C0886A5409C729CC2136EDAFF4B2ACE693A@IAD2MBX09.mex02.mlsrvr.com> We have had our slide printer for going on 6 years and have had minimal problems. It does require good maintenance habits though, as do all slide printers. We also purchased the PM/service contract on this and the cassette printer when the warranty ran out and I think they are worth the cost since service calls always seem to cost a lot even if it is something minor. Regards, Carol Carol Wilson, HT(ASCP) Associate Scientist III Team Leader/Histopathology Ricerca Biosciences, LLC From mw <@t> personifysearch.com Wed Oct 23 07:15:07 2013 From: mw <@t> personifysearch.com (Matt Ward) Date: Wed Oct 23 07:15:17 2013 Subject: [Histonet] New Technical Sales Opportunity - IHC Antibodies Message-ID: <049101cecfe9$84e8c520$8eba4f60$@personifysearch.com> Good morning, We have had a world leading cancer diagnostics client open a new opportunity in the Southwest. Our client is searching for an Antibody Sales Specialist ideally based in TX, OK, AR, LA, or AZ. We are looking for histology professionals who have a strong background in IHC and antibodies, to drive antibodies sales in the region. Please contact me directly to learn more about the opportunity. mw@personifysearch.com , or 800.875.6188 ext. 103 Thank you, Matt Ward Account Executive Personify 5020 Weston Parkway Suite 315 Cary NC 27513 (Tel) 800.875.6188 direct ext 103 (Fax) 919.460.0642 www.personifysearch.com From CDavis <@t> che-east.org Wed Oct 23 07:51:06 2013 From: CDavis <@t> che-east.org (Davis, Cassie) Date: Wed Oct 23 07:51:14 2013 Subject: [Histonet] Shedding biopsies Message-ID: <08861B9CF6C7774E874635A4818AE37B013EA7450A@CHEXCMS01.one.ads.che.org> Hi Diana, RE: "In the past when we receive these samples there has been no issues cutting them. Over the past few months there has been a tendency for these blocks to shred into fine strips when cut. Almost like there was sand in the blocks. Mollifex, decal, warm or cold, doesn't matter they still shred. Getting levels is such a challenge. I have inquired at the collection site to determine if there is a change in the process of collecting that could cause this. I have ensured the samples are not left on gauze or something to allow them to dry out. Even blocks with lots of blood and/or mucous will shred as well as blocks with only a scanty amount present." There was a problem a few/many years ago with a paraffin manufacture using a cardboard conveyor belt for the pellets. Apparently, small pieces of cardboard making it's way in with the paraffin. You can check your melting tub and see if there is a tan residual in the bottom this will let you know if "foreign" material may be responsible for your problem. Cassandra Davis Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From lcolbert <@t> pathmdlabs.com Wed Oct 23 09:59:52 2013 From: lcolbert <@t> pathmdlabs.com (Laurie Colbert) Date: Wed Oct 23 10:04:12 2013 Subject: [Histonet] slide drying oven Message-ID: <12ECD7346266D74691EC2BFC75285E452F362040@BFL323E10.pathmdlabs.local> Can anyone recommend a small, forced air slide drying oven? Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab From foreightl <@t> gmail.com Wed Oct 23 11:35:05 2013 From: foreightl <@t> gmail.com (Patrick Laurie) Date: Wed Oct 23 11:35:11 2013 Subject: [Histonet] slide drying oven In-Reply-To: <12ECD7346266D74691EC2BFC75285E452F362040@BFL323E10.pathmdlabs.local> References: <12ECD7346266D74691EC2BFC75285E452F362040@BFL323E10.pathmdlabs.local> Message-ID: Hi Laurie, Biocare has one that I have used, they call it the desert chamber pro, one of the few that is setup and designed expressly for drying slides. It works very well, and has different heating options and a timer. However, all you may need is a basic mechanical convection oven, Boekel has a couple of what they call digital incubators with a fan that can go from ambient to 90 degrees. Finally, if cost is a consideration, I've purchased from Affordableovens.com. I got a relatively inexpensive mechanical convection oven for $1500 that works well. Good luck, Patrick Laurie Celligent Diagnostics Charlotte, NC On Wed, Oct 23, 2013 at 10:59 AM, Laurie Colbert wrote: > Can anyone recommend a small, forced air slide drying oven? > > Laurie Colbert, HT (ASCP) > Histology Supervisor > PATH MD > 8158 Beverly Blvd. > Los Angeles, CA 90048 > (323) 648-3214 direct > (424) 245-7284 main lab > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie(HT)ASCP QIHC Histology Manager Celligent Diagnostics, LLC 101 East W.T. Harris Blvd | Suite 1212 | Charlotte, NC 28262 Work: 704-970-3300 Cell: 704-266-0869 From Vickroy.Jim <@t> mhsil.com Wed Oct 23 12:16:17 2013 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Wed Oct 23 12:16:26 2013 Subject: [Histonet] Amacr antibody from DAKO Message-ID: I am checking into ordering the prediluted amacr antibody from DAKO since it is an IVD. Could anyone share the protocol they are using on the Benchmark Ultra with this antibody and the standard I-view detection kit? James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From joelleweaver <@t> hotmail.com Wed Oct 23 12:35:06 2013 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Wed Oct 23 12:35:10 2013 Subject: [Histonet] HTL / CG In-Reply-To: References: Message-ID: Hello fellow histology netters I have been asked by my employer to search for and retain an individual who possesses an HTL (ASCP) certification, but who also has a CG (ASCP) certification ( or at least have solid experience in the arena of FISH & cytogenetics). I have not personally come across anyone like that in my own personal, mostly clinical histology career. Perhaps it is more common in research? Can anyone offer an opinion or insight into how common the above combination of education, training and certification(s) may be? I tried to contact the BOR/BOC for a non-identified statistic on that, but have not gotten a reply. Appreciate any assistance. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: McKenzie.Emily@mhsil.com > To: histonet@lists.utsouthwestern.edu > Date: Wed, 16 Oct 2013 19:31:20 -0500 > Subject: [Histonet] Desperately seeking information!!! > > Hello all, > A few weeks ago I sent out an information seeking email regarding IHC turnaround time. I did not get much in the way of responses. I figured there was not enough information provided to answer the general questions I was asking. I am having trouble obtaining an national average for IHC turnaround time. > I am wondering if all you fellow histoneters out there would be willing to give me some info so I can see were we stand in comparison to facilities of similar size. The facility I work at turns out anywhere from 90-150 IHC stained slides daily. We have an average of 160 cases with around 700 H&E stained slides daily. I have listed a few questions below, if any of you would be so kind as to take the time to answer them it would be greatly appreciated. > > What is the rough estimate of cases and initial H&E stained slides that are turned out daily? > > Roughly, how many IHC stained slides do you turn out in a day? > > On average, what is your IHC turnaround time? > > What tissues are you working with (general surgical, dermatology's, research etc)? > > How many techs do you have that can perform IHC staining? > > Who is your instrumentation through? > > At the end of the day/run, is there a stain log printed? > > If so, who signs off on the positive/negative? > > If there are any other processes/procedures you feel are imperative to your IHC turnaround time please feel free to comment or offer suggestions. > > Thank you for taking the time to help us to improve our processes. If you have any questions or concerns please let me know. > Again, thank you for your help, > > > Emily K. McKenzie BS, HT(ASCP) > > Memorial Medical Center?701 North First Street?Springfield, IL 62781 > Ph: 217-788-3991?email: McKenzie.Emily@mhsil.com > > > > > ________________________________ > This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Vickroy.Jim <@t> mhsil.com Wed Oct 23 12:37:07 2013 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Wed Oct 23 12:37:11 2013 Subject: [Histonet] ASR antibodies verses IVD antibodies Message-ID: In older CAP regulations ASR's had to be handled separately than IVD antibodies including a disclaimer acquired from the FDA. I don't find that in the current CAP checklist. Has this been eliminated and if so how are people handling validation of ASR antibodies? Are they using the validation they use for new antibodies introduced to the lab? Jim James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From patrick.lewis <@t> seattlechildrens.org Wed Oct 23 12:54:19 2013 From: patrick.lewis <@t> seattlechildrens.org (Lewis, Patrick) Date: Wed Oct 23 12:54:29 2013 Subject: [Histonet] Can someone recommend a good labeling pen for cryotubes. Message-ID: <3903BE18914F4440834F0E620415FFCA381ECFA3@PPWEXD01a.childrens.sea.kids> Hi everyone, Can someone recommend a good pen to use to label cryo tubes. The VWR pens we have been using are apparently discontinued. I would like something that writes as fine as an ultrafine sharpie, but is less likely to smear and is more long lasting, and solvent tolerant. Thanks Patrick. CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From Joyce.Weems <@t> emoryhealthcare.org Wed Oct 23 12:56:55 2013 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Wed Oct 23 12:57:02 2013 Subject: [Histonet] RE: Can someone recommend a good labeling pen for cryotubes. In-Reply-To: <3903BE18914F4440834F0E620415FFCA381ECFA3@PPWEXD01a.childrens.sea.kids> References: <3903BE18914F4440834F0E620415FFCA381ECFA3@PPWEXD01a.childrens.sea.kids> Message-ID: We have had good luck with the Statmark Pen from StatLab - Item # SMP-BK. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lewis, Patrick Sent: Wednesday, October 23, 2013 1:54 PM To: 'Histonet@lists.utsouthwestern.edu' Subject: [Histonet] Can someone recommend a good labeling pen for cryotubes. Hi everyone, Can someone recommend a good pen to use to label cryo tubes. The VWR pens we have been using are apparently discontinued. I would like something that writes as fine as an ultrafine sharpie, but is less likely to smear and is more long lasting, and solvent tolerant. Thanks Patrick. CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From Timothy.Morken <@t> ucsfmedctr.org Wed Oct 23 12:58:55 2013 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Wed Oct 23 12:59:11 2013 Subject: [Histonet] RE: ASR antibodies verses IVD antibodies In-Reply-To: References: Message-ID: <761E2B5697F795489C8710BCC72141FF0A7B50@ex07.net.ucsf.edu> Jim, Since companies are not allowed, by law, to tell you any method information about ASR's, it is the responsibility of the lab to do a comprehensive validation according to CLIA regulations. Treat it as if it were an antibody some researcher gave you and you need to figure out EVERYTHING to complete a validation: specificity and sensitivity, reproducibility, etc. That all has to be documented. Tim Morken UCSF Pathology -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Wednesday, October 23, 2013 10:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ASR antibodies verses IVD antibodies In older CAP regulations ASR's had to be handled separately than IVD antibodies including a disclaimer acquired from the FDA. I don't find that in the current CAP checklist. Has this been eliminated and if so how are people handling validation of ASR antibodies? Are they using the validation they use for new antibodies introduced to the lab? Jim James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wbenton <@t> cua.md Wed Oct 23 12:58:47 2013 From: wbenton <@t> cua.md (Walter Benton) Date: Wed Oct 23 13:00:21 2013 Subject: [Histonet] RE: Can someone recommend a good labeling pen for cryotubes. In-Reply-To: <3903BE18914F4440834F0E620415FFCA381ECFA3@PPWEXD01a.childrens.sea.kids> References: <3903BE18914F4440834F0E620415FFCA381ECFA3@PPWEXD01a.childrens.sea.kids> Message-ID: <0B8979A204680A42B93A52B486088CD93931ABEF87@CUAEXH1.GCU-MD.local> Are you using the red label "Industrial Sharpie" already, if not give them a try? I would also suggest using a slide marking pen since they are resistant to many solvents used in the lab like alcohol and xylene. Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) ChesapeakeUrology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lewis, Patrick [patrick.lewis@seattlechildrens.org] Sent: Wednesday, October 23, 2013 1:54 PM To: 'Histonet@lists.utsouthwestern.edu' Subject: [Histonet] Can someone recommend a good labeling pen for cryotubes. Hi everyone, Can someone recommend a good pen to use to label cryo tubes. The VWR pens we have been using are apparently discontinued. I would like something that writes as fine as an ultrafine sharpie, but is less likely to smear and is more long lasting, and solvent tolerant. Thanks Patrick. CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From one_angel_secret <@t> yahoo.com Wed Oct 23 13:21:46 2013 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Wed Oct 23 13:22:20 2013 Subject: [Histonet] CLIA In-Reply-To: <0E828EC51C7CC445A51E53F81B64E8C737A2D2@s-irv-exchmb.PathologyPartners.intranet> References: <0E828EC51C7CC445A51E53F81B64E8C737A2D2@s-irv-exchmb.PathologyPartners.intranet> Message-ID: I can only speak from a path lab point of view and from reading the IQCP guidelines it doesn't allow you to use these new measures for path. Path is exempt. and in path I've always used controls for each test. My understanding is in some specialties they are going to let them make individualized qc measures if they want to do those risk assessments etc. I'm still going over it but this is the just of what I see so far. Hope this helps. Sent from my iPhone On Oct 22, 2013, at 10:23 AM, "Hale, Meredith" wrote: > Has anyone out there been told by CLIA you must run a negative control along with a positive control for HP special stains , not IHC ? I know we do this for AFB but never have for an HP special stain . > > Also the new IQCP CLIA is rolling out it states that if your lab doesn't enroll in the voluntary IQCP that you must perform tow external controls on each test system for each day . Is this what they may be referring to ? Thanks in advance . > > > > Meredith Hale HT (ASCP)cm > Director External Sales Support > > Miraca Life Sciences > 6655 North MacArthur Blvd. > Irving , Texas 75039 > Office: 214-596-2219 > Cell: 469-648-8253 > Fax: 1-866-688-3280 > mhale@miracals.com> > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mhale <@t> MiracaLS.com Wed Oct 23 13:24:01 2013 From: mhale <@t> MiracaLS.com (Hale, Meredith) Date: Wed Oct 23 13:24:07 2013 Subject: [Histonet] CLIA In-Reply-To: References: <0E828EC51C7CC445A51E53F81B64E8C737A2D2@s-irv-exchmb.PathologyPartners.intranet> Message-ID: <0E828EC51C7CC445A51E53F81B64E8C737E797@s-irv-exchmb.PathologyPartners.intranet> Thanks Kim, I agree seems more clinical ... -----Original Message----- From: Kim Donadio [mailto:one_angel_secret@yahoo.com] Sent: Wednesday, October 23, 2013 1:22 PM To: Hale, Meredith Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] CLIA I can only speak from a path lab point of view and from reading the IQCP guidelines it doesn't allow you to use these new measures for path. Path is exempt. and in path I've always used controls for each test. My understanding is in some specialties they are going to let them make individualized qc measures if they want to do those risk assessments etc. I'm still going over it but this is the just of what I see so far. Hope this helps. Sent from my iPhone On Oct 22, 2013, at 10:23 AM, "Hale, Meredith" wrote: > Has anyone out there been told by CLIA you must run a negative control along with a positive control for HP special stains , not IHC ? I know we do this for AFB but never have for an HP special stain . > > Also the new IQCP CLIA is rolling out it states that if your lab doesn't enroll in the voluntary IQCP that you must perform tow external controls on each test system for each day . Is this what they may be referring to ? Thanks in advance . > > > > Meredith Hale HT (ASCP)cm > Director External Sales Support > > Miraca Life Sciences > 6655 North MacArthur Blvd. > Irving , Texas 75039 > Office: 214-596-2219 > Cell: 469-648-8253 > Fax: 1-866-688-3280 > mhale@miracals.com> > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From foreightl <@t> gmail.com Wed Oct 23 14:27:32 2013 From: foreightl <@t> gmail.com (Patrick Laurie) Date: Wed Oct 23 14:27:41 2013 Subject: [Histonet] RE: Can someone recommend a good labeling pen for cryotubes. In-Reply-To: <0B8979A204680A42B93A52B486088CD93931ABEF87@CUAEXH1.GCU-MD.local> References: <3903BE18914F4440834F0E620415FFCA381ECFA3@PPWEXD01a.childrens.sea.kids> <0B8979A204680A42B93A52B486088CD93931ABEF87@CUAEXH1.GCU-MD.local> Message-ID: I second the statmark pens from stat-lab. They are very solvent resistant, don't dry out that easy. They are however a little on the pricey side, at about $5.00 a pen. Patrick Laurie On Wed, Oct 23, 2013 at 1:58 PM, Walter Benton wrote: > Are you using the red label "Industrial Sharpie" already, if not give them > a try? > > I would also suggest using a slide marking pen since they are resistant to > many solvents used in the lab like alcohol and xylene. > > Walter Benton HT(ASCP)QIHC > Histology Supervisor > Chesapeake Urology Associates > 806 Landmark Drive, Suite 127 > Glen Burnie, MD 21061 > 443-471-5850 (Direct) > 410-768-5961 (Lab) > 410-768-5965 (Fax) > ChesapeakeUrology.com > > Voted a Best Place to Work by > Baltimore and Modern Healthcare > Magazines. > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [ > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lewis, Patrick [ > patrick.lewis@seattlechildrens.org] > Sent: Wednesday, October 23, 2013 1:54 PM > To: 'Histonet@lists.utsouthwestern.edu' > Subject: [Histonet] Can someone recommend a good labeling pen for > cryotubes. > > Hi everyone, > > Can someone recommend a good pen to use to label cryo tubes. The VWR > pens we have been using are apparently discontinued. > > I would like something that writes as fine as an ultrafine sharpie, but is > less likely to smear and is more long lasting, and solvent tolerant. > > Thanks > > Patrick. > > > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is > for the sole use of the intended recipient(s) and may contain confidential > and privileged information protected by law. Any unauthorized review, use, > disclosure or distribution is prohibited. If you are not the intended > recipient, please contact the sender by reply e-mail and destroy all copies > of the original message. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > CONFIDENTIALITY NOTICE: The information contained in this electronic > message is intended solely for the personal and confidential use of the > designated recipient(s) named above and may contain information that is > protected from disclosure under applicable law. If you are not the > intended recipient, or the employee or agent responsible for delivering it > to the intended recipient, you are hereby notified that any dissemination, > distribution or copying of this transmission is strictly prohibited. If you > have received this transmission in error, please notify the transmitting > person/department immediately by email or telephone (410) 581-5881 and > delete the message without making a copy. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie(HT)ASCP QIHC Histology Manager Celligent Diagnostics, LLC 101 East W.T. Harris Blvd | Suite 1212 | Charlotte, NC 28262 Work: 704-970-3300 Cell: 704-266-0869 From joelleweaver <@t> hotmail.com Wed Oct 23 14:58:25 2013 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Wed Oct 23 14:58:31 2013 Subject: [Histonet] HTL / CG In-Reply-To: <7FF33CF7105F23409F31E748D906B489A02678E66C@R04BYNMSGB2.r04.med.va.gov> References: , , <7FF33CF7105F23409F31E748D906B489A02678E66C@R04BYNMSGB2.r04.med.va.gov> Message-ID: Thank you for the information and insight. You are indeed multi-talented. Sounds like whatever direction you go, you will be successful. I am not expecting to find such a combination as HTL/CG, with extensive experience easily. I just wanted to have some numbers, opinions, and information to return if it takes a long while- and some kind of explanation for being empty-handed when them come asking. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: Taylor.Clifford@va.gov > To: joelleweaver@hotmail.com > Date: Wed, 23 Oct 2013 15:22:34 -0400 > Subject: RE: [Histonet] HTL / CG > > I would say pretty uncommon! > > I graduated with my Bachelor's in Agricultural Biotechnology and as a side bar did my AAS in Histotechnology so my current supervisor was incredibly pleased with the fact that I had a solid background in both biomolecular science and current biotechnology techniques as well as the histology/histotechnology background. He's the PI of a neuropathology lab where he has done basic and advanced immunohistochemistry and various biomolecular testing for 40+ years and he said I was the first HT to also have the biotechnology background. > > I graduated from SUNY Cobleskill where Dr. Colony has been the program director for a number of years and I was also her first student to do both programs (Benefitted the program greatly to have a student tutor the following year on campus since most students finish their AAS and are gone). > I have looked into continuing my education for the CG certification but I'm still getting my foot in the door here at the research lab and studying for GRE's for entrance into either a PhD or DVM program so I don't want to add any more to my plate at the moment! > > Good luck on your search!! > > > Taylor CM Clifford > Research Associate > Albany Research Institute > 113 Holland Avenue > Albany, NY 12208 > 518-626-5664 > Taylor.Clifford@va.gov > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelle weaver > Sent: Wednesday, October 23, 2013 1:35 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] HTL / CG > > Hello fellow histology netters > I have been asked by my employer to search for and retain an individual who possesses an HTL (ASCP) certification, but who also has a CG (ASCP) certification ( or at least have solid experience in the arena of FISH & cytogenetics). I have not personally come across anyone like that in my own personal, mostly clinical histology career. Perhaps it is more common in research? > > Can anyone offer an opinion or insight into how common the above combination of education, training and certification(s) may be? > I tried to contact the BOR/BOC for a non-identified statistic on that, but have not gotten a reply. > Appreciate any assistance. > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > From: McKenzie.Emily@mhsil.com > > To: histonet@lists.utsouthwestern.edu > > Date: Wed, 16 Oct 2013 19:31:20 -0500 > > Subject: [Histonet] Desperately seeking information!!! > > > > Hello all, > > A few weeks ago I sent out an information seeking email regarding IHC turnaround time. I did not get much in the way of responses. I figured there was not enough information provided to answer the general questions I was asking. I am having trouble obtaining an national average for IHC turnaround time. > > I am wondering if all you fellow histoneters out there would be willing to give me some info so I can see were we stand in comparison to facilities of similar size. The facility I work at turns out anywhere from 90-150 IHC stained slides daily. We have an average of 160 cases with around 700 H&E stained slides daily. I have listed a few questions below, if any of you would be so kind as to take the time to answer them it would be greatly appreciated. > > > > What is the rough estimate of cases and initial H&E stained slides that are turned out daily? > > > > Roughly, how many IHC stained slides do you turn out in a day? > > > > On average, what is your IHC turnaround time? > > > > What tissues are you working with (general surgical, dermatology's, research etc)? > > > > How many techs do you have that can perform IHC staining? > > > > Who is your instrumentation through? > > > > At the end of the day/run, is there a stain log printed? > > > > If so, who signs off on the positive/negative? > > > > If there are any other processes/procedures you feel are imperative to your IHC turnaround time please feel free to comment or offer suggestions. > > > > Thank you for taking the time to help us to improve our processes. If you have any questions or concerns please let me know. > > Again, thank you for your help, > > > > > > Emily K. McKenzie BS, HT(ASCP) > > > > Memorial Medical Center?701 North First Street?Springfield, IL 62781 > > Ph: 217-788-3991?email: McKenzie.Emily@mhsil.com > > > > > > > > > > ________________________________ > > This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Wed Oct 23 15:16:36 2013 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Wed Oct 23 15:16:49 2013 Subject: [Histonet] HTL / CG In-Reply-To: References: Message-ID: <761E2B5697F795489C8710BCC72141FF0A7BD9@ex07.net.ucsf.edu> Joelle, I think you will have a very hard time finding someone with both, especially dual ASCP certification. I've met or hear of anyone with dual certification, or even working in both. It may be the best you will find is someone who has certification in one and some practical experience in the other. Or someone willing to cross-train. Good luck! Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelle weaver Sent: Wednesday, October 23, 2013 10:35 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HTL / CG Hello fellow histology netters I have been asked by my employer to search for and retain an individual who possesses an HTL (ASCP) certification, but who also has a CG (ASCP) certification ( or at least have solid experience in the arena of FISH & cytogenetics). I have not personally come across anyone like that in my own personal, mostly clinical histology career. Perhaps it is more common in research? Can anyone offer an opinion or insight into how common the above combination of education, training and certification(s) may be? I tried to contact the BOR/BOC for a non-identified statistic on that, but have not gotten a reply. Appreciate any assistance. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: McKenzie.Emily@mhsil.com > To: histonet@lists.utsouthwestern.edu > Date: Wed, 16 Oct 2013 19:31:20 -0500 > Subject: [Histonet] Desperately seeking information!!! > > Hello all, > A few weeks ago I sent out an information seeking email regarding IHC turnaround time. I did not get much in the way of responses. I figured there was not enough information provided to answer the general questions I was asking. I am having trouble obtaining an national average for IHC turnaround time. > I am wondering if all you fellow histoneters out there would be willing to give me some info so I can see were we stand in comparison to facilities of similar size. The facility I work at turns out anywhere from 90-150 IHC stained slides daily. We have an average of 160 cases with around 700 H&E stained slides daily. I have listed a few questions below, if any of you would be so kind as to take the time to answer them it would be greatly appreciated. > > What is the rough estimate of cases and initial H&E stained slides that are turned out daily? > > Roughly, how many IHC stained slides do you turn out in a day? > > On average, what is your IHC turnaround time? > > What tissues are you working with (general surgical, dermatology's, research etc)? > > How many techs do you have that can perform IHC staining? > > Who is your instrumentation through? > > At the end of the day/run, is there a stain log printed? > > If so, who signs off on the positive/negative? > > If there are any other processes/procedures you feel are imperative to your IHC turnaround time please feel free to comment or offer suggestions. > > Thank you for taking the time to help us to improve our processes. If you have any questions or concerns please let me know. > Again, thank you for your help, > > > Emily K. McKenzie BS, HT(ASCP) > > Memorial Medical Center?701 North First Street?Springfield, IL 62781 > Ph: 217-788-3991?email: McKenzie.Emily@mhsil.com > > > > > ________________________________ > This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From algranth <@t> email.arizona.edu Wed Oct 23 17:01:04 2013 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Wed Oct 23 17:01:41 2013 Subject: [Histonet] cutting thick brain frozen sections for Golgi stain Message-ID: <8EFB117C-B86F-46E0-8557-0CAB684521D5@email.arizona.edu> I need to cut 80 micron sections of frozen brain. Tried today at -18 degrees C but the brains kept cracking. I read that the temp should be -3 to -13 degrees C. Has anybody cut sections this thick of brain? Can you pass along any words of inspiration? Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 From Klaus.Kruttwig <@t> ucsf.edu Wed Oct 23 17:22:29 2013 From: Klaus.Kruttwig <@t> ucsf.edu (Kruttwig, Klaus) Date: Wed Oct 23 17:22:41 2013 Subject: [Histonet] RE: cutting thick brain frozen sections for Golgi stain In-Reply-To: <8EFB117C-B86F-46E0-8557-0CAB684521D5@email.arizona.edu> References: <8EFB117C-B86F-46E0-8557-0CAB684521D5@email.arizona.edu> Message-ID: Hi Andrea, did you try it with a vibratome? If not, I would recommend it. Thanks, Klaus ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Grantham, Andrea L - (algranth) [algranth@email.arizona.edu] Sent: Wednesday, October 23, 2013 3:01 PM To: HISTONET Subject: [Histonet] cutting thick brain frozen sections for Golgi stain I need to cut 80 micron sections of frozen brain. Tried today at -18 degrees C but the brains kept cracking. I read that the temp should be -3 to -13 degrees C. Has anybody cut sections this thick of brain? Can you pass along any words of inspiration? Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lentwistle <@t> ucsd.edu Wed Oct 23 19:02:48 2013 From: lentwistle <@t> ucsd.edu (Entwistle, Laura) Date: Wed Oct 23 19:05:23 2013 Subject: [Histonet] RE: cutting thick brain frozen sections for Golgi stain In-Reply-To: References: <8EFB117C-B86F-46E0-8557-0CAB684521D5@email.arizona.edu> Message-ID: I routinely cut 50 or even 200um sections. The brain should have some variations of light beige to it. If it is too white, it is too cold. What are you using? I have found that a microtome works much better for thicker slices than a cryostat. Good luck! Laura -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kruttwig, Klaus Sent: Wednesday, October 23, 2013 3:22 PM To: Grantham, Andrea L - (algranth); HISTONET Subject: [Histonet] RE: cutting thick brain frozen sections for Golgi stain Hi Andrea, did you try it with a vibratome? If not, I would recommend it. Thanks, Klaus ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Grantham, Andrea L - (algranth) [algranth@email.arizona.edu] Sent: Wednesday, October 23, 2013 3:01 PM To: HISTONET Subject: [Histonet] cutting thick brain frozen sections for Golgi stain I need to cut 80 micron sections of frozen brain. Tried today at -18 degrees C but the brains kept cracking. I read that the temp should be -3 to -13 degrees C. Has anybody cut sections this thick of brain? Can you pass along any words of inspiration? Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ggracie <@t> stvincents.com.au Wed Oct 23 20:28:34 2013 From: ggracie <@t> stvincents.com.au (Gary Gracie) Date: Wed Oct 23 20:29:04 2013 Subject: [Histonet] Low Temp problem Message-ID: <6b31d66bf60d43f7b100c9ae7c190926@SVMHSEXCH02.svmhs.stvincents.com.au> I am also running Ventana Benchmark Ultras. This low temp problem has started to occur regularly to individual heater pads (five in total over several weeks, across two machines). Ventana have promptly replaced the pad on each occasion. Gary Gracie Senior Technical Officer IHC Laboratory Anatomical Pathology St Vincents Hospital Sydney, Australia Message: 1 Date: Tue, 22 Oct 2013 11:04:45 -0700 From: "HERRINGTON, SHEILA" Subject: RE: [Histonet] Low Temp question To: 'Tom McNemar' , 'Shawn Leslie ' , "Histonet@lists.utsouthwestern.edu" Message-ID: <9D5F3F245A5FE0458E46E9A71E3EE08C06CD37F350@DC1SERV352.interiorhealth.ca> Content-Type: text/plain; charset="utf-8" I also have been having low temp errors. I use Benchmark Ultras and they are not a batch stainer, so pads are heated independently. I noticed the staining on the particular pad with the error was not quite to the usual standard so I have stopped using that pad. Have contacted Ventana for a case number, but have not got an answer. Is this a new problem for you as well? This only started a few weeks ago for us. Sheila Herrington Technical Lead Histopathology and Immunohistochemistry Kelowna General Hospital 2268 Pandosy Street, Kelowna, B.C. V1Y 1T2 250-862-4300 ext 7587 or 7510 Sheila.herrington@interiorhealth.ca -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Tuesday, October 22, 2013 8:28 AM To: 'Shawn Leslie '; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Low Temp question We see these continually as well. We are told that it is a software glitch and not really a problem with the heat. I was told that they are working on it. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shawn Leslie Sent: Tuesday, October 22, 2013 11:07 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Low Temp question Goodmorning all, I have a question concerning the Ventana Benchmark XT. We are constantly having low temperature errors during almost every run. The immunos look fine but we are still concerned. Has anyone else that uses the Benchmark experienced this also ? We have noticed that the silver coating on the heating pads is wearing off. Ventana indicated to us that it's nothing to be concerned about. Any hel p would be appreciated. Shawn Leslie HT (ASCP) ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the system manager. This footnote also confirms that this email message has been virus scanned and although no viruses were detected by the system, St Vincents & Mater Health Sydney accepts no liability for any consequential damage resulting from email containing any computer viruses. ********************************************************************** From TMcNemar <@t> lmhealth.org Thu Oct 24 04:40:33 2013 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Thu Oct 24 04:42:56 2013 Subject: [Histonet] RE: Low Temp problem In-Reply-To: <6b31d66bf60d43f7b100c9ae7c190926@SVMHSEXCH02.svmhs.stvincents.com.au> References: <6b31d66bf60d43f7b100c9ae7c190926@SVMHSEXCH02.svmhs.stvincents.com.au> Message-ID: We have had pads replaced as well but the problem continues. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gary Gracie Sent: Wednesday, October 23, 2013 9:29 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Low Temp problem I am also running Ventana Benchmark Ultras. This low temp problem has started to occur regularly to individual heater pads (five in total over several weeks, across two machines). Ventana have promptly replaced the pad on each occasion. Gary Gracie Senior Technical Officer IHC Laboratory Anatomical Pathology St Vincents Hospital Sydney, Australia Message: 1 Date: Tue, 22 Oct 2013 11:04:45 -0700 From: "HERRINGTON, SHEILA" Subject: RE: [Histonet] Low Temp question To: 'Tom McNemar' , 'Shawn Leslie ' , "Histonet@lists.utsouthwestern.edu" Message-ID: <9D5F3F245A5FE0458E46E9A71E3EE08C06CD37F350@DC1SERV352.interiorhealth.ca> Content-Type: text/plain; charset="utf-8" I also have been having low temp errors. I use Benchmark Ultras and they are not a batch stainer, so pads are heated independently. I noticed the staining on the particular pad with the error was not quite to the usual standard so I have stopped using that pad. Have contacted Ventana for a case number, but have not got an answer. Is this a new problem for you as well? This only started a few weeks ago for us. Sheila Herrington Technical Lead Histopathology and Immunohistochemistry Kelowna General Hospital 2268 Pandosy Street, Kelowna, B.C. V1Y 1T2 250-862-4300 ext 7587 or 7510 Sheila.herrington@interiorhealth.ca -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Tuesday, October 22, 2013 8:28 AM To: 'Shawn Leslie '; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Low Temp question We see these continually as well. We are told that it is a software glitch and not really a problem with the heat. I was told that they are working on it. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shawn Leslie Sent: Tuesday, October 22, 2013 11:07 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Low Temp question Goodmorning all, I have a question concerning the Ventana Benchmark XT. We are constantly having low temperature errors during almost every run. The immunos look fine but we are still concerned. Has anyone else that uses the Benchmark experienced this also ? We have noticed that the silver coating on the heating pads is wearing off. Ventana indicated to us that it's nothing to be concerned about. Any hel p would be appreciated. Shawn Leslie HT (ASCP) ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the system manager. This footnote also confirms that this email message has been virus scanned and although no viruses were detected by the system, St Vincents & Mater Health Sydney accepts no liability for any consequential damage resulting from email containing any computer viruses. ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From borisa <@t> trinity-health.org Thu Oct 24 06:09:50 2013 From: borisa <@t> trinity-health.org (Anthony F. Boris) Date: Thu Oct 24 06:10:01 2013 Subject: [Histonet] Breast core needle biopsy procedure question. Message-ID: I am sending this on behalf of our Director. Thanks in advance for any and all responses. Tony Boris I am the Medical Director of Surgical Pathology at St. Joseph Mercy Hospital Oakland, located in Pontiac MI. I staff our weekly breast cancer treatment planning conference and am responsible for overseeing breast cancer ancillary marker testing. I am writing to see if you could share your AP procedure for processing core needle biopsies of breast tissue. I believe that we have a good process for assuring proper fixation on our cores and excisions. We sometimes run out of tissue in the core when working up a positive case, though. Our current procedure is to do H&E's at 3 levels on all breast cores. The reviewing pathologist can then order additional IHC as needed for diagnosis, followed by additional sections for ER, PR and Her2 IHC, followed by additional sections for FISH sendout if necessary. We try to anticipate what may be needed and cut blanks for ancillary markers as soon as possible so as not to exhaust diagnostic tissue, but occasionally fail at that. This morning one of my associates was faced with just that situation, leading to reconsideration of our SOP for the breast cores. Should we look at just one H&E section, for instance, and based on that either get deeper H&E to exclude something significant that is not present in the first section, or get additional diagnostic and prognostic studies done beginning at level 2 followed by an H&E at the end of the deck? At any rate, I wanted to know what the routine procedure is for handling these cores at U of M. I would appreciate it if you could forward a copy of your AP procedure, or outline the same in an email Thank you for your time. Sincerely, Jim Furlong, MD From Melissa.Kuhnla <@t> chsli.org Thu Oct 24 06:35:10 2013 From: Melissa.Kuhnla <@t> chsli.org (Kuhnla, Melissa) Date: Thu Oct 24 06:35:25 2013 Subject: [Histonet] Low Temp problem In-Reply-To: <6b31d66bf60d43f7b100c9ae7c190926@SVMHSEXCH02.svmhs.stvincents.com.au> References: <6b31d66bf60d43f7b100c9ae7c190926@SVMHSEXCH02.svmhs.stvincents.com.au> Message-ID: We are also having similar issues. Our instruments are about five years old. I wonder if at a certain point they just start to fade out..?.. Very interesting to see the issue here on histonet. Must be more common that we realize. Melissa Kuhnla Lead Technologist for IHC and ISH Catholic Health Services of Long Island Hauppauge, NY 11788 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gary Gracie Sent: Wednesday, October 23, 2013 9:29 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Low Temp problem I am also running Ventana Benchmark Ultras. This low temp problem has started to occur regularly to individual heater pads (five in total over several weeks, across two machines). Ventana have promptly replaced the pad on each occasion. Gary Gracie Senior Technical Officer IHC Laboratory Anatomical Pathology St Vincents Hospital Sydney, Australia Message: 1 Date: Tue, 22 Oct 2013 11:04:45 -0700 From: "HERRINGTON, SHEILA" Subject: RE: [Histonet] Low Temp question To: 'Tom McNemar' , 'Shawn Leslie ' , "Histonet@lists.utsouthwestern.edu" Message-ID: <9D5F3F245A5FE0458E46E9A71E3EE08C06CD37F350@DC1SERV352.interiorhealth.ca > Content-Type: text/plain; charset="utf-8" I also have been having low temp errors. I use Benchmark Ultras and they are not a batch stainer, so pads are heated independently. I noticed the staining on the particular pad with the error was not quite to the usual standard so I have stopped using that pad. Have contacted Ventana for a case number, but have not got an answer. Is this a new problem for you as well? This only started a few weeks ago for us. Sheila Herrington Technical Lead Histopathology and Immunohistochemistry Kelowna General Hospital 2268 Pandosy Street, Kelowna, B.C. V1Y 1T2 250-862-4300 ext 7587 or 7510 Sheila.herrington@interiorhealth.ca -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Tuesday, October 22, 2013 8:28 AM To: 'Shawn Leslie '; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Low Temp question We see these continually as well. We are told that it is a software glitch and not really a problem with the heat. I was told that they are working on it. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shawn Leslie Sent: Tuesday, October 22, 2013 11:07 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Low Temp question Goodmorning all, I have a question concerning the Ventana Benchmark XT. We are constantly having low temperature errors during almost every run. The immunos look fine but we are still concerned. Has anyone else that uses the Benchmark experienced this also ? We have noticed that the silver coating on the heating pads is wearing off. Ventana indicated to us that it's nothing to be concerned about. Any hel p would be appreciated. Shawn Leslie HT (ASCP) ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the system manager. This footnote also confirms that this email message has been virus scanned and although no viruses were detected by the system, St Vincents & Mater Health Sydney accepts no liability for any consequential damage resulting from email containing any computer viruses. ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. From debgranato <@t> yahoo.com Thu Oct 24 07:15:20 2013 From: debgranato <@t> yahoo.com (Debbie Granato) Date: Thu Oct 24 07:15:25 2013 Subject: [Histonet] Grossing Prostate Biopsies Message-ID: For anyone who grosses prostate needle biopsies- does anyone place 2 sites in one cassette , marking one site with ink to identify the site? I would appreciate any opinions or ideas on this technique. Debbie Granato HT(ASCP) Sent from my iPhone From LRaff <@t> uropartners.com Thu Oct 24 07:22:15 2013 From: LRaff <@t> uropartners.com (Lester Raff MD) Date: Thu Oct 24 07:22:19 2013 Subject: [Histonet] Grossing Prostate Biopsies In-Reply-To: References: Message-ID: Oppenheimer Urology Lab does that. I don't like the idea, but I guess it is a cost savings. We do not do it here. Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0230 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Debbie Granato Sent: Thursday, October 24, 2013 7:15 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Grossing Prostate Biopsies For anyone who grosses prostate needle biopsies- does anyone place 2 sites in one cassette , marking one site with ink to identify the site? I would appreciate any opinions or ideas on this technique. Debbie Granato HT(ASCP) Sent from my iPhone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akbitting <@t> geisinger.edu Thu Oct 24 07:29:20 2013 From: akbitting <@t> geisinger.edu (Bitting, Angela K.) Date: Thu Oct 24 07:29:26 2013 Subject: [Histonet] Low Temp problem In-Reply-To: References: <6b31d66bf60d43f7b100c9ae7c190926@SVMHSEXCH02.svmhs.stvincents.com.au> Message-ID: <77F52EFAB8B1694B885E277C48FCD0F64CEA5009@GHSEXMBX1W8K1V.geisinger.edu> It happens to us too. Ours aren't that old though. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kuhnla, Melissa Sent: Thursday, October 24, 2013 7:35 AM To: Gary Gracie; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Low Temp problem We are also having similar issues. Our instruments are about five years old. I wonder if at a certain point they just start to fade out..?.. Very interesting to see the issue here on histonet. Must be more common that we realize. Melissa Kuhnla Lead Technologist for IHC and ISH Catholic Health Services of Long Island Hauppauge, NY 11788 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gary Gracie Sent: Wednesday, October 23, 2013 9:29 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Low Temp problem I am also running Ventana Benchmark Ultras. This low temp problem has started to occur regularly to individual heater pads (five in total over several weeks, across two machines). Ventana have promptly replaced the pad on each occasion. Gary Gracie Senior Technical Officer IHC Laboratory Anatomical Pathology St Vincents Hospital Sydney, Australia Message: 1 Date: Tue, 22 Oct 2013 11:04:45 -0700 From: "HERRINGTON, SHEILA" Subject: RE: [Histonet] Low Temp question To: 'Tom McNemar' , 'Shawn Leslie ' , "Histonet@lists.utsouthwestern.edu" Message-ID: <9D5F3F245A5FE0458E46E9A71E3EE08C06CD37F350@DC1SERV352.interiorhealth.ca > Content-Type: text/plain; charset="utf-8" I also have been having low temp errors. I use Benchmark Ultras and they are not a batch stainer, so pads are heated independently. I noticed the staining on the particular pad with the error was not quite to the usual standard so I have stopped using that pad. Have contacted Ventana for a case number, but have not got an answer. Is this a new problem for you as well? This only started a few weeks ago for us. Sheila Herrington Technical Lead Histopathology and Immunohistochemistry Kelowna General Hospital 2268 Pandosy Street, Kelowna, B.C. V1Y 1T2 250-862-4300 ext 7587 or 7510 Sheila.herrington@interiorhealth.ca -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Tuesday, October 22, 2013 8:28 AM To: 'Shawn Leslie '; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Low Temp question We see these continually as well. We are told that it is a software glitch and not really a problem with the heat. I was told that they are working on it. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shawn Leslie Sent: Tuesday, October 22, 2013 11:07 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Low Temp question Goodmorning all, I have a question concerning the Ventana Benchmark XT. We are constantly having low temperature errors during almost every run. The immunos look fine but we are still concerned. Has anyone else that uses the Benchmark experienced this also ? We have noticed that the silver coating on the heating pads is wearing off. Ventana indicated to us that it's nothing to be concerned about. Any hel p would be appreciated. Shawn Leslie HT (ASCP) ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the system manager. This footnote also confirms that this email message has been virus scanned and although no viruses were detected by the system, St Vincents & Mater Health Sydney accepts no liability for any consequential damage resulting from email containing any computer viruses. ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. From KSimeone <@t> leavittmgt.com Thu Oct 24 07:41:04 2013 From: KSimeone <@t> leavittmgt.com (Delray Beach Pathology Kari Simeone) Date: Thu Oct 24 07:41:39 2013 Subject: [Histonet] Part time Histo Tech position DELRAY BEACH, FLORIDA Message-ID: Hi Histonetters! We are looking for a part time tech here in our very busy Delray Derm Lab. This is a permanent part time position with full time growth potential. PLEASE NO HEAD HUNTERS/PLACEMENT SERVICES!!! Email your resume to ksimeone@leavittmgt.com if interested. *part time position Mon-Wed 5p-10p (starting time semi-flexible) also vacation coverage shifts available *MUST be licensed as a FL histotechnologist or technician *MUST have at least 2 years experience (dermatology preferred) *very proficient in embedding and microtomy *experience in grossing and immunohistochemistry (BOND) a plus *must be self motivated and a team player *knowledge in operating Ventana and Leica equipment desired (not necessary) Kari M Simeone Histology/Immunohistochemistry Specialist Supervisor Alternate Laboratory Supervisor ADCS Clinics, LLC Advanced Dermatology and Cosmetic Surgery Ameriderm ksimeone@leavittmgt.com www.advancedderm.com From blgaylord <@t> ael.com Thu Oct 24 08:24:19 2013 From: blgaylord <@t> ael.com (Brian L. Gaylord) Date: Thu Oct 24 08:24:32 2013 Subject: [Histonet] RE: HTL / CG (joelle weaver) Message-ID: <89D631A52B5AAF4FBB65E0E96B5A32DCB8CD92@s-ex02.americanesoterics.com> I agree that the best you will probably find is someone with certification in one discipline that will be willing to train in the other. I am a CT and HTL and even though the fields are so closely related, people like me with both certs (and experience) are very few. Brian Gaylord CT, HTL (ASCP) (QIHC) Histology Supervisor AEL Memphis 1701 Century Center Cove Memphis, TN 38134 (901) 432-8550 This message contains privileged and confidential information intended only for the use of the addressee named above. If you are not the intended recipient of this message you must not disseminate, copy or take any action in reliance on it." From rsrichmond <@t> gmail.com Thu Oct 24 09:22:47 2013 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Thu Oct 24 09:22:50 2013 Subject: [Histonet] Re: ASR antibodies verses IVD antibodies Message-ID: Jim Vickroy in US area code 217 asks for "ASR antibodies verses IVD antibodies" so here are some verses for the occasion. (Happy Friday Eve!) What's an ASR? What's an IVD? (I do know FDA and sometimes CAP.) Endless abbreviations leave me with frustrations. Please, spell it out, for this aging lout! ("In older CAP regulations ASR's had to be handled separately than IVD antibodies including a disclaimer acquired from the FDA. I don't find that in the current CAP checklist.") Bob Richmond Samurai Pathologist Maryville TN From algranth <@t> email.arizona.edu Thu Oct 24 10:06:44 2013 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Thu Oct 24 10:08:10 2013 Subject: [Histonet] RE: cutting thick brain frozen sections for Golgi stain In-Reply-To: <1382625551.15098.YahooMailNeo@web162102.mail.bf1.yahoo.com> References: <8EFB117C-B86F-46E0-8557-0CAB684521D5@email.arizona.edu> <1382625551.15098.YahooMailNeo@web162102.mail.bf1.yahoo.com> Message-ID: <55414E20-2CF1-453F-B541-FB0828F3D1C0@email.arizona.edu> I'm going to try sectioning at higher temps today and I hope that it works. Thanks all for the suggestions. We used to have a vibratome but the thing only worked right for certain kinds of samples. I don't see us getting another one since the occasion to use something like that happens very infrequently. Too bad. Yes, the brains were fixed - treated with mercury and have a gray-green cast to them (wearing gloves). They were also sucrose cryoprotected. Andi Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 From eshoy <@t> flash.net Thu Oct 24 10:40:09 2013 From: eshoy <@t> flash.net (Eric Hoy) Date: Thu Oct 24 10:40:17 2013 Subject: [Histonet] Re: ASR antibodies verses IVD antibodies In-Reply-To: Message-ID: Dr. Richmond, I frequently share your frustration with acronyms and abbreviations, but these are two that I actually know. ASR is Analyte Specific Reagent, a term that is applied to many of the monoclonal antibodies that we use in the clinical immunology lab, and also to antibodies used in Immunohistochemistry (IHC). This means that the manufacturer hasn't done the studies necessary to get FDA (Finicky Dumb Aristocrats) clearance to sell it as an In Vitro Diagnostic (IVD), and so each lab that wants to use it has to go through a process of validation to show that the reagent does what we want it to do. Another layer of bureaucracy added to our lives to "improve" the quality of what we do. Regards, Eric Hoy (another aging lout) =================================================== Eric S. Hoy, Ph.D., SI(ASCP) Clinical Associate Professor Department of Health Care Sciences The University of Texas Southwestern Medical Center Dallas, Texas Email: eshoy@flash.net =================================================== On 10/24/13 9:22 AM, "Bob Richmond" wrote: > Jim Vickroy in US area code 217 asks for "ASR antibodies verses IVD > antibodies" so here are some verses for the occasion. (Happy Friday Eve!) > > What's an ASR? > What's an IVD? > (I do know FDA > and sometimes CAP.) > Endless abbreviations > leave me with frustrations. > Please, spell it out, > for this aging lout! > > ("In older CAP regulations ASR's had to be handled separately than IVD > antibodies including a disclaimer acquired from the FDA. I don't find that > in the current CAP checklist.") > > Bob Richmond > Samurai Pathologist > Maryville TN > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TJohnson <@t> gnf.org Thu Oct 24 10:41:39 2013 From: TJohnson <@t> gnf.org (Teri Johnson) Date: Thu Oct 24 10:41:44 2013 Subject: [Histonet] Re: cutting thick brain frozen section for Golgi stain Message-ID: <9F3CFEE76E51B64991C7485270890B40497D8B63@EX5.lj.gnf.org> Hi Andi, I second the option for Vibrotome section. I think that will be your best bet and should provide the least amount of artifact. If you must use the cryostat, I agree that -18 is way too cold, go warmer than you are ordinarily comfortable with. If the OCT can't handle it, you might try using gelatin/sucrose as an embedding medium. Will you be staining free-floating sections? Teri Johnson Manager, Histology Genomics Institute for Novartis Research Foundation San Diego, CA 858-332-4752 From algranth <@t> email.arizona.edu Thu Oct 24 10:55:00 2013 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Thu Oct 24 10:55:26 2013 Subject: [Histonet] Re: cutting thick brain frozen section for Golgi stain In-Reply-To: <9F3CFEE76E51B64991C7485270890B40497D8B63@EX5.lj.gnf.org> References: <9F3CFEE76E51B64991C7485270890B40497D8B63@EX5.lj.gnf.org> Message-ID: I wish I could do free floating sections! These are mounted on a slide. 8-( Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 From madeathridge <@t> pastnashville.com Thu Oct 24 11:50:55 2013 From: madeathridge <@t> pastnashville.com (Mary Ann Deathridge) Date: Thu Oct 24 11:51:45 2013 Subject: [Histonet] Texas Red Message-ID: <64f204eb$34930784$397f78c5$@com> 1) Does anybody use a "Texas Red filter" for viewing IHC slides stained with Sox10, red chromagen? I have used several different co.'s red chromagen. We get positive staining. However, if we get as vibrant as my pathologist likes then there is a slight blush. He returned from a meeting and said that "Texas Red" was being used. I found it to be a filter for the scope. The filters cost approx. $600-900. Thoughts? 2) Anyone using ERG antibody for dermpath cases? Vendor? Thanks in advance ! ! ! ! Hi Joyce, hope you are doing well (^_^) Maryann Deathridge, BS, HT (ASCP) Lab Manager Pathology Assoc. of St. Thomas Nashville, TN 37205 615-298-4100, Fax: 615-298-4141 From TJohnson <@t> gnf.org Thu Oct 24 12:17:32 2013 From: TJohnson <@t> gnf.org (Teri Johnson) Date: Thu Oct 24 12:17:36 2013 Subject: [Histonet] Re: Texas Red Message-ID: <9F3CFEE76E51B64991C7485270890B40497D8BD8@EX5.lj.gnf.org> Dear Mary Ann, I'll (hopefully) beat Gayle Callis to the punch on red chromogen/fluorescent detection. According to her experience, you need to slightly understain the red chromogen to achieve the best fluorescent signal. I don't know if it has to do with saturation but too much red deposition = less fluorescence. We also use a Texas Red excitation filter on our scope. Texas Red is a fluorophore used for labeling in IHC (among other things), and the filter is one that will excite at that wavelength. It also excites the Alexa594 fluorophore which is what we use extensively. Teri Johnson Manager, Histology Genomics Institute for Novartis Research Foundation San Diego, CA 858-332-4752 From candice_camille <@t> yahoo.com Thu Oct 24 12:41:33 2013 From: candice_camille <@t> yahoo.com (Candice Smoots) Date: Thu Oct 24 12:41:37 2013 Subject: [Histonet] cutting thick brain frozen sections for Golgi stain In-Reply-To: <8EFB117C-B86F-46E0-8557-0CAB684521D5@email.arizona.edu> References: <8EFB117C-B86F-46E0-8557-0CAB684521D5@email.arizona.edu> Message-ID: <1382636493.33540.YahooMailNeo@web165001.mail.bf1.yahoo.com> Hi ? I often section brain sections at 50 microns. However, I use a sliding microtome. For me, those are the best at sectioning thick brain sections. Vibratomes are great too. I would use the cryostat as a last resort. I also agree that if it is too cold, it wont section correctly and will begin to crack. Monitor the tem,p and make sure that it is not too cold. I remain yours truely, Candice Camille ________________________________ From: "Grantham, Andrea L - (algranth)" To: HISTONET Sent: Wednesday, October 23, 2013 5:01 PM Subject: [Histonet] cutting thick brain frozen sections for Golgi stain I need to cut 80 micron sections of frozen brain. Tried today at -18 degrees C but the brains kept cracking. I read that the temp should be -3 to -13 degrees C. Has anybody cut sections this thick of brain? Can you pass along any words of inspiration? Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415? ? Fax: 520.626.2097 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Connie.Dieringer <@t> va.gov Thu Oct 24 13:10:25 2013 From: Connie.Dieringer <@t> va.gov (Dieringer, Connie J.) Date: Thu Oct 24 13:11:54 2013 Subject: [Histonet] Granulocyte Colony Stimulating Factor testing Message-ID: Does anyone know of a reference lab that performs staining or any testing on tissue for Granulocyte Colony Stimulating Factor? I have found labs that perform the testing on blood, but haven't been able to locate one yet that does this testing on tissue. Thank you! Connie Dieringer, HTL (ASCP) Histology Supervisor Michael E. DeBakey VA Medical Center Houston, TX Rm. 3A-215A, Bldg. 100 Tel.: 713-794-7259 Pager: 281-551-0171 Email: connie.dieringer@va.gov From HornHV <@t> archildrens.org Thu Oct 24 13:44:42 2013 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Thu Oct 24 13:44:47 2013 Subject: [Histonet] freezing spray Message-ID: <25A4DE08332B19499904459F00AAACB719C704FE84@EVS1.archildrens.org> Our hospital has not allowed freezing spray to be used in the frozen section lab for many years. We now have a new group of doctors who want to use the spray. The docs think the frozen sections take too long to freeze. Yet, they meet the frozen section TAT for more than 98% of our cases. I think it's worth not using it for the one case where no one suspects TB but the patient will be positive. Do you allow freezing spray in your frozen section lab? Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From trathborne <@t> somerset-healthcare.com Thu Oct 24 14:01:51 2013 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Thu Oct 24 14:02:19 2013 Subject: [Histonet] RE: freezing spray In-Reply-To: <25A4DE08332B19499904459F00AAACB719C704FE84@EVS1.archildrens.org> References: <25A4DE08332B19499904459F00AAACB719C704FE84@EVS1.archildrens.org> Message-ID: <3AD061FE740D464FAC7BF6B5CFB75707A8AE24F6@smcmail02.somerset-healthcare.com> If they are hospital employees, you should have a little more say in what goes on in the lab. Is there a policy which addresses this? If not, now might be a good opportunity to draft one and have the Medical Director sign off on it. You can also get your Infection Control department involved if you need additional support. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Thursday, October 24, 2013 2:45 PM To: histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] freezing spray Our hospital has not allowed freezing spray to be used in the frozen section lab for many years. We now have a new group of doctors who want to use the spray. The docs think the frozen sections take too long to freeze. Yet, they meet the frozen section TAT for more than 98% of our cases. I think it's worth not using it for the one case where no one suspects TB but the patient will be positive. Do you allow freezing spray in your frozen section lab? Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Thu Oct 24 14:28:05 2013 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Thu Oct 24 14:28:10 2013 Subject: [Histonet] HTL / CG In-Reply-To: References: , , , , Message-ID: Mark Sounds like I will be in similar circumstance soon. I am not a molecular person and I am not certified in molecular OR cytogenetics. Just HTL/QIHC, I do have degrees but it never made that much difference really so far. I have been trained in FISH and FISH enumeration (barely- just starting). I do the IHC and ISH, routine histology, specials, training, hiring, SOP writing, validation, purchasing, CAP stuff, etc. I am a bench Histotechnologist only. Believe me, all they ever say to me is that they wish I knew more cytogenetics/FISH, flow cytometry and/or PCR. So seems to me, that whatever you know/do outside histology DOES in fact put you in higher demand. Bravo to you. Sure you don't want to come and help me out? Joelle Weaver MAOM, HTL (ASCP) QIHC Date: Thu, 24 Oct 2013 11:55:28 -0700 Subject: Re: [Histonet] HTL / CG From: marktarango@gmail.com To: joelleweaver@hotmail.com haha I hope that is true. I'm the de facto lead tech in my department and I'm trying to get everyone up to speed on cutting and FISH pretreatment/scoring. Its going pretty well. I think it's VERY helpful to have someone who can move between both areas. Histology asked me to cut the molecular orders today since they're short-handed. I love helping out in histology and IHC. If the pay was right I would move about anywhere but I don't know that I'm exactly what you're looking for. I don't have my BA/BS and am not certified in molecular. I also don't have experience in conventional cytogenetics (g-banding). I'm also not the best PCR tech although I help out in that area too. Mark On Wed, Oct 23, 2013 at 4:04 PM, joelle weaver wrote: That is what they want, but they also want a BA/BS and prefer certification. Yes, I had a feeling it is pretty rare. You must be in HIGH demand. Do you perform manually? have a scanner? do the slide analysis and scoring? Joelle Weaver MAOM, HTL (ASCP) QIHC Date: Wed, 23 Oct 2013 15:41:24 -0700 Subject: Re: [Histonet] HTL / CG From: marktarango@gmail.com To: joelleweaver@hotmail.com I'm not certified as a CG(ASCP) but I do FISH all day long on tissue and cell based preps. I would say it's pretty uncommon to find someone who has a molecular and histology background. On Wed, Oct 23, 2013 at 10:35 AM, joelle weaver wrote: Hello fellow histology netters I have been asked by my employer to search for and retain an individual who possesses an HTL (ASCP) certification, but who also has a CG (ASCP) certification ( or at least have solid experience in the arena of FISH & cytogenetics). I have not personally come across anyone like that in my own personal, mostly clinical histology career. Perhaps it is more common in research? Can anyone offer an opinion or insight into how common the above combination of education, training and certification(s) may be? I tried to contact the BOR/BOC for a non-identified statistic on that, but have not gotten a reply. Appreciate any assistance. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: McKenzie.Emily@mhsil.com > To: histonet@lists.utsouthwestern.edu > Date: Wed, 16 Oct 2013 19:31:20 -0500 > Subject: [Histonet] Desperately seeking information!!! > > Hello all, > A few weeks ago I sent out an information seeking email regarding IHC turnaround time. I did not get much in the way of responses. I figured there was not enough information provided to answer the general questions I was asking. I am having trouble obtaining an national average for IHC turnaround time. > I am wondering if all you fellow histoneters out there would be willing to give me some info so I can see were we stand in comparison to facilities of similar size. The facility I work at turns out anywhere from 90-150 IHC stained slides daily. We have an average of 160 cases with around 700 H&E stained slides daily. I have listed a few questions below, if any of you would be so kind as to take the time to answer them it would be greatly appreciated. > > What is the rough estimate of cases and initial H&E stained slides that are turned out daily? > > Roughly, how many IHC stained slides do you turn out in a day? > > On average, what is your IHC turnaround time? > > What tissues are you working with (general surgical, dermatology's, research etc)? > > How many techs do you have that can perform IHC staining? > > Who is your instrumentation through? > > At the end of the day/run, is there a stain log printed? > > If so, who signs off on the positive/negative? > > If there are any other processes/procedures you feel are imperative to your IHC turnaround time please feel free to comment or offer suggestions. > > Thank you for taking the time to help us to improve our processes. If you have any questions or concerns please let me know. > Again, thank you for your help, > > > Emily K. McKenzie BS, HT(ASCP) > > Memorial Medical Center?701 North First Street?Springfield, IL 62781 > Ph: 217-788-3991?email: McKenzie.Emily@mhsil.com > > > > > ________________________________ > This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Thu Oct 24 18:22:26 2013 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Thu Oct 24 18:22:40 2013 Subject: [Histonet] freezing spray In-Reply-To: <25A4DE08332B19499904459F00AAACB719C704FE84@EVS1.archildrens.org> References: <25A4DE08332B19499904459F00AAACB719C704FE84@EVS1.archildrens.org> Message-ID: Reasons against the use of freezing spray for frozen sections: From the Federal Registry 1910.1030: ?All procedures involving blood or other potential infectious materials shall be performed in such a manner as to minimize splashing, spraying, spattering, or generation of droplets of these substances.? From NCCLS (now CLSI) Document M29-T: ?Frozen sections done on unfixed tissue pose a high risk because accidents are common. Freezing of tissue does not inactivate infectious agents. Freezing propellants under pressure should not be used for frozen sections as they may cause spattering of droplets of infectious material.? From: "Horn, Hazel V" To: "histonet (histonet@lists.utsouthwestern.edu)" Date: 10/24/2013 11:47 AM Subject: [Histonet] freezing spray Sent by: histonet-bounces@lists.utsouthwestern.edu Our hospital has not allowed freezing spray to be used in the frozen section lab for many years. We now have a new group of doctors who want to use the spray. The docs think the frozen sections take too long to freeze. Yet, they meet the frozen section TAT for more than 98% of our cases. I think it's worth not using it for the one case where no one suspects TB but the patient will be positive. Do you allow freezing spray in your frozen section lab? Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From leah_simmons22 <@t> hotmail.com Thu Oct 24 20:33:43 2013 From: leah_simmons22 <@t> hotmail.com (Leah Simmons) Date: Thu Oct 24 20:33:51 2013 Subject: [Histonet] Microtome Blade safety, in or out when not in use? Message-ID: Hello all :-) I am doing a quick microtome blade safety survey, When you finish work, do you leave your blade in the microtome behind the blade guard or do you take it out? If you take it out and it is a new blade or a blade still useful for trimming where do you store it? Thank you for your feedback, I really appreciate it. Regards Leah Simmons From chesarato <@t> hotmail.com Thu Oct 24 21:14:58 2013 From: chesarato <@t> hotmail.com (Cesar Francisco Romero) Date: Thu Oct 24 21:15:02 2013 Subject: [Histonet] RE: Grossing Prostate Biopsies Message-ID: I gross prostate needle biopsies every week. I receive 12 samples but convert them in 6 paraffin blocks. I stain the Medial Samples of each level and each side with Eosin and the Lateral Samples with EA - 36 .Example: Base Lateral Right ( EA - 36 ), with Base Medial Right ( Eosin ).Base Lateral Left ( EA - 36 ), with Base Medial Left ( Eosin ) and so on. I wash the excess of colorant with 96 alcohol, wrap the samples of each sextant in one paper and put them in one cassette. Then I put the Cassette in the First Alcohol to avoid washing the Stains with aqueous formalin. It is very important to cut the cylinders so they do not exceed 5 mm. long. You avoid the coiling and facilitate the embedding. After all that it is very easy to see the colors in the paraffin block.It saves time and money and there is no errors if you are a tidy quiet person. Cesar RomeroBuenos AiresArgentina From JMacDonald <@t> mtsac.edu Thu Oct 24 22:32:20 2013 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Thu Oct 24 22:32:06 2013 Subject: [Histonet] Microtome Blade safety, in or out when not in use? In-Reply-To: References: Message-ID: We save the blade to use for trimming. We store the blade in a plastic 5-slide mailer. From: Leah Simmons To: "histonet@lists.utsouthwestern.edu" Date: 10/24/2013 06:36 PM Subject: [Histonet] Microtome Blade safety, in or out when not in use? Sent by: histonet-bounces@lists.utsouthwestern.edu Hello all :-) I am doing a quick microtome blade safety survey, When you finish work, do you leave your blade in the microtome behind the blade guard or do you take it out? If you take it out and it is a new blade or a blade still useful for trimming where do you store it? Thank you for your feedback, I really appreciate it. Regards Leah Simmons _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TMcNemar <@t> lmhealth.org Fri Oct 25 04:41:09 2013 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Fri Oct 25 04:41:21 2013 Subject: [Histonet] RE: freezing spray In-Reply-To: <25A4DE08332B19499904459F00AAACB719C704FE84@EVS1.archildrens.org> References: <25A4DE08332B19499904459F00AAACB719C704FE84@EVS1.archildrens.org> Message-ID: We do not us it. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Thursday, October 24, 2013 2:45 PM To: histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] freezing spray Our hospital has not allowed freezing spray to be used in the frozen section lab for many years. We now have a new group of doctors who want to use the spray. The docs think the frozen sections take too long to freeze. Yet, they meet the frozen section TAT for more than 98% of our cases. I think it's worth not using it for the one case where no one suspects TB but the patient will be positive. Do you allow freezing spray in your frozen section lab? Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From patpxs <@t> gmail.com Fri Oct 25 06:02:47 2013 From: patpxs <@t> gmail.com (Paula Sicurello) Date: Fri Oct 25 06:02:54 2013 Subject: [Histonet] Microtome Blade safety, in or out when not in use? In-Reply-To: References: Message-ID: Left in but covered with the blade guard. Not spanking new, but usable (for facing) get stored in an old box that the slides came in. I like the slide mailer idea, and will switch to that. -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 HIPAA Privacy Notification: This message and any accompanying documents are covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, and contain information intended for the specific individual (s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying or the taking of any action based on the contents of this information is strictly prohibited . If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. On Thu, Oct 24, 2013 at 9:33 PM, Leah Simmons wrote: > Hello all :-) > I am doing a quick microtome blade safety survey, > When you finish work, do you leave your blade in the microtome behind the > blade guard or do you take it out? > If you take it out and it is a new blade or a blade still useful for > trimming where do you store it? > Thank you for your feedback, I really appreciate it. > Regards > Leah Simmons > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Sherrian.McAnn <@t> va.gov Fri Oct 25 08:46:09 2013 From: Sherrian.McAnn <@t> va.gov (McAnn, Sherrian) Date: Fri Oct 25 08:46:48 2013 Subject: [Histonet] Microtome Blade safety, in or out when not in use? In-Reply-To: References: Message-ID: <61E2B58CECEF384094A363989D47C0900A53078F@VHAV17MSGA2.v17.med.va.gov> I was taught that when leaving your microtome for any length of time to always take the blade out. We had a tech that had the habit of leaving the blade on her microtome and even though she had the "safety guard" up someone from biomed still managed to lean on it and get cut (go figure) If I want to save a slightly used blade to maybe trim with the next time then I will put it into a slide mailer (plastic with attached lid) but that is a safety issue . The safety officer says that when a blade is used and taken out then it should be thrown away..because the more it is being handled the greater the chance of injury. Just saying what they told me. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello Sent: Friday, October 25, 2013 6:03 AM To: Leah Simmons Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Microtome Blade safety, in or out when not in use? Left in but covered with the blade guard. Not spanking new, but usable (for facing) get stored in an old box that the slides came in. I like the slide mailer idea, and will switch to that. -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 HIPAA Privacy Notification: This message and any accompanying documents are covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, and contain information intended for the specific individual (s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying or the taking of any action based on the contents of this information is strictly prohibited . If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. On Thu, Oct 24, 2013 at 9:33 PM, Leah Simmons wrote: > Hello all :-) > I am doing a quick microtome blade safety survey, When you finish > work, do you leave your blade in the microtome behind the blade guard > or do you take it out? > If you take it out and it is a new blade or a blade still useful for > trimming where do you store it? > Thank you for your feedback, I really appreciate it. > Regards > Leah Simmons > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PAMarcum <@t> uams.edu Fri Oct 25 10:04:41 2013 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Fri Oct 25 10:04:51 2013 Subject: [Histonet] Microtome Blade safety, in or out when not in use? In-Reply-To: <61E2B58CECEF384094A363989D47C0900A53078F@VHAV17MSGA2.v17.med.va.gov> References: <61E2B58CECEF384094A363989D47C0900A53078F@VHAV17MSGA2.v17.med.va.gov> Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA32D315E6F3@Mail2Node2.ad.uams.edu> The rule here is" a blade is cheaper than a cut". Anytime you are walking away and returning within a few minutes to cut use the knife guard otherwise throw the blade out. It is an accident looking to happen. Recently we had a tech decide not use the knife guard and seriously cut himself by misjudging the distance from his elbow reaching an knife he was no longer able to use. Pam Marcum -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of McAnn, Sherrian Sent: Friday, October 25, 2013 8:46 AM To: Paula Sicurello; Leah Simmons Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Microtome Blade safety, in or out when not in use? I was taught that when leaving your microtome for any length of time to always take the blade out. We had a tech that had the habit of leaving the blade on her microtome and even though she had the "safety guard" up someone from biomed still managed to lean on it and get cut (go figure) If I want to save a slightly used blade to maybe trim with the next time then I will put it into a slide mailer (plastic with attached lid) but that is a safety issue . The safety officer says that when a blade is used and taken out then it should be thrown away..because the more it is being handled the greater the chance of injury. Just saying what they told me. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello Sent: Friday, October 25, 2013 6:03 AM To: Leah Simmons Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Microtome Blade safety, in or out when not in use? Left in but covered with the blade guard. Not spanking new, but usable (for facing) get stored in an old box that the slides came in. I like the slide mailer idea, and will switch to that. -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 HIPAA Privacy Notification: This message and any accompanying documents are covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, and contain information intended for the specific individual (s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying or the taking of any action based on the contents of this information is strictly prohibited . If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. On Thu, Oct 24, 2013 at 9:33 PM, Leah Simmons wrote: > Hello all :-) > I am doing a quick microtome blade safety survey, When you finish > work, do you leave your blade in the microtome behind the blade guard > or do you take it out? > If you take it out and it is a new blade or a blade still useful for > trimming where do you store it? > Thank you for your feedback, I really appreciate it. > Regards > Leah Simmons > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From thisisann <@t> aol.com Fri Oct 25 10:27:54 2013 From: thisisann <@t> aol.com (Ann Specian) Date: Fri Oct 25 10:27:57 2013 Subject: [Histonet] ANP 23045 The performance of all instruments and equipment is verified before use. Message-ID: <8D09FADE8F20F63-13E8-9C64@webmail-d159.sysops.aol.com> Has anyone written a procedure for this new checklist item? If so, what "procedure" are you using to verify equipment/instrument performance prior to use? Ann From dellav <@t> musc.edu Fri Oct 25 10:28:48 2013 From: dellav <@t> musc.edu (della Speranza, Vinnie) Date: Fri Oct 25 10:28:55 2013 Subject: [Histonet] hands free scalpel blade system ? Message-ID: I would appreciate hearing from anyone who is using a hands-free scalpel blade system that works well. If you are not familiar with what I referring to, we are looking to a means of removing used scalpel blades from scalpel handles and installing new blades hands free, without the need to touch the blades. We currently use the Bladex system but are searching for something that works more reliably. If you know of an alternative to Bladex I would appreciate hearing from you. Thank you, Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Ave. MSC 908 Charleston, SC 29425 Ph. 843-792-6353 Fax. 843-7928974 From Sherrian.McAnn <@t> va.gov Fri Oct 25 11:34:39 2013 From: Sherrian.McAnn <@t> va.gov (McAnn, Sherrian) Date: Fri Oct 25 11:35:10 2013 Subject: [Histonet] Microtome Blade safety, in or out when not in use? In-Reply-To: <41D3A1AF6FEF0643BDC89E0516A6EA32D315E6F3@Mail2Node2.ad.uams.edu> References: <61E2B58CECEF384094A363989D47C0900A53078F@VHAV17MSGA2.v17.med.va.gov> <41D3A1AF6FEF0643BDC89E0516A6EA32D315E6F3@Mail2Node2.ad.uams.edu> Message-ID: <61E2B58CECEF384094A363989D47C0900A530845@VHAV17MSGA2.v17.med.va.gov> AMEN TO THAT! -----Original Message----- From: Marcum, Pamela A [mailto:PAMarcum@uams.edu] Sent: Friday, October 25, 2013 10:05 AM To: McAnn, Sherrian; Paula Sicurello; Leah Simmons Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Microtome Blade safety, in or out when not in use? The rule here is" a blade is cheaper than a cut". Anytime you are walking away and returning within a few minutes to cut use the knife guard otherwise throw the blade out. It is an accident looking to happen. Recently we had a tech decide not use the knife guard and seriously cut himself by misjudging the distance from his elbow reaching an knife he was no longer able to use. Pam Marcum -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of McAnn, Sherrian Sent: Friday, October 25, 2013 8:46 AM To: Paula Sicurello; Leah Simmons Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Microtome Blade safety, in or out when not in use? I was taught that when leaving your microtome for any length of time to always take the blade out. We had a tech that had the habit of leaving the blade on her microtome and even though she had the "safety guard" up someone from biomed still managed to lean on it and get cut (go figure) If I want to save a slightly used blade to maybe trim with the next time then I will put it into a slide mailer (plastic with attached lid) but that is a safety issue . The safety officer says that when a blade is used and taken out then it should be thrown away..because the more it is being handled the greater the chance of injury. Just saying what they told me. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello Sent: Friday, October 25, 2013 6:03 AM To: Leah Simmons Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Microtome Blade safety, in or out when not in use? Left in but covered with the blade guard. Not spanking new, but usable (for facing) get stored in an old box that the slides came in. I like the slide mailer idea, and will switch to that. -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 HIPAA Privacy Notification: This message and any accompanying documents are covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, and contain information intended for the specific individual (s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying or the taking of any action based on the contents of this information is strictly prohibited . If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. On Thu, Oct 24, 2013 at 9:33 PM, Leah Simmons wrote: > Hello all :-) > I am doing a quick microtome blade safety survey, When you finish > work, do you leave your blade in the microtome behind the blade guard > or do you take it out? > If you take it out and it is a new blade or a blade still useful for > trimming where do you store it? > Thank you for your feedback, I really appreciate it. > Regards > Leah Simmons > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From Timothy.Morken <@t> ucsfmedctr.org Fri Oct 25 11:43:05 2013 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Fri Oct 25 11:43:21 2013 Subject: [Histonet] Microtome Blade safety, in or out when not in use? In-Reply-To: References: Message-ID: <761E2B5697F795489C8710BCC72141FF0A8203@ex07.net.ucsf.edu> I always take it out. You never know if someone will come along and do something... In our lab the mircrotomes do not strictly "belong" to any particular tech, and they are used by different people at different times of day or different shifts. Tim Morken Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Leah Simmons Sent: Thursday, October 24, 2013 6:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Microtome Blade safety, in or out when not in use? Hello all :-) I am doing a quick microtome blade safety survey, When you finish work, do you leave your blade in the microtome behind the blade guard or do you take it out? If you take it out and it is a new blade or a blade still useful for trimming where do you store it? Thank you for your feedback, I really appreciate it. Regards Leah Simmons _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TJohnson <@t> gnf.org Fri Oct 25 12:21:05 2013 From: TJohnson <@t> gnf.org (Teri Johnson) Date: Fri Oct 25 12:21:12 2013 Subject: [Histonet] Re: freezing spray Message-ID: <9F3CFEE76E51B64991C7485270890B40497D8FF5@EX5.lj.gnf.org> Hazel, Jennifer Mac provided the best answer as to why they should not use it. Apparently "because I said so" isn't working. Maybe give them the "Mom-look" when saying it? Seriously, they have other options if they want to freeze the samples quicker. We always had a dewar with liquid nitrogen in our grossing room available for doing snap freezing. A quick dunk in that should do the trick. You can bring in some dry ice and that might work as well. Quicker than slow freezing in the cryostat, but not as quick as snap freezing. Newer cryostats have a quick freeze platform with peltier cooling, so updating an old cryostat for safety reasons might be another option. Teri Johnson Manager, Histology Genomics Institute for Novartis Research Foundation San Diego, CA 858-332-4752 From tpodawiltz <@t> lrgh.org Fri Oct 25 12:45:18 2013 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Fri Oct 25 12:45:24 2013 Subject: [Histonet] Microtome Blade safety, in or out when not in use? NO PHI In-Reply-To: <61E2B58CECEF384094A363989D47C0900A530845@VHAV17MSGA2.v17.med.va.gov> References: <61E2B58CECEF384094A363989D47C0900A53078F@VHAV17MSGA2.v17.med.va.gov> <41D3A1AF6FEF0643BDC89E0516A6EA32D315E6F3@Mail2Node2.ad.uams.edu> <61E2B58CECEF384094A363989D47C0900A530845@VHAV17MSGA2.v17.med.va.gov> Message-ID: <38667E7FB77ECD4E91BFAEB8D986386325010F1DA4@LRGHEXVS1.practice.lrgh.org> We do not re-use the blades or leave them in the microtome it is against our safety practices. We handle the blades twice, when we place them in the blade holder and when we remove them and drop them into the sharps container. Tom Podawiltz HT (ASCP) Histology Section Head/Laboratory Safety Officer. LRGHealthcare Laconia, NH 03246 603-524-3211 ext: 3220 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of McAnn, Sherrian Sent: Friday, October 25, 2013 12:35 PM To: Marcum, Pamela A; Paula Sicurello; Leah Simmons Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Microtome Blade safety, in or out when not in use? AMEN TO THAT! -----Original Message----- From: Marcum, Pamela A [mailto:PAMarcum@uams.edu] Sent: Friday, October 25, 2013 10:05 AM To: McAnn, Sherrian; Paula Sicurello; Leah Simmons Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Microtome Blade safety, in or out when not in use? The rule here is" a blade is cheaper than a cut". Anytime you are walking away and returning within a few minutes to cut use the knife guard otherwise throw the blade out. It is an accident looking to happen. Recently we had a tech decide not use the knife guard and seriously cut himself by misjudging the distance from his elbow reaching an knife he was no longer able to use. Pam Marcum -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of McAnn, Sherrian Sent: Friday, October 25, 2013 8:46 AM To: Paula Sicurello; Leah Simmons Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Microtome Blade safety, in or out when not in use? I was taught that when leaving your microtome for any length of time to always take the blade out. We had a tech that had the habit of leaving the blade on her microtome and even though she had the "safety guard" up someone from biomed still managed to lean on it and get cut (go figure) If I want to save a slightly used blade to maybe trim with the next time then I will put it into a slide mailer (plastic with attached lid) but that is a safety issue . The safety officer says that when a blade is used and taken out then it should be thrown away..because the more it is being handled the greater the chance of injury. Just saying what they told me. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello Sent: Friday, October 25, 2013 6:03 AM To: Leah Simmons Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Microtome Blade safety, in or out when not in use? Left in but covered with the blade guard. Not spanking new, but usable (for facing) get stored in an old box that the slides came in. I like the slide mailer idea, and will switch to that. -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 HIPAA Privacy Notification: This message and any accompanying documents are covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, and contain information intended for the specific individual (s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying or the taking of any action based on the contents of this information is strictly prohibited . If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. On Thu, Oct 24, 2013 at 9:33 PM, Leah Simmons wrote: > Hello all :-) > I am doing a quick microtome blade safety survey, When you finish > work, do you leave your blade in the microtome behind the blade guard > or do you take it out? > If you take it out and it is a new blade or a blade still useful for > trimming where do you store it? > Thank you for your feedback, I really appreciate it. > Regards > Leah Simmons > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From Joyce.Weems <@t> emoryhealthcare.org Fri Oct 25 13:07:02 2013 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Fri Oct 25 13:07:12 2013 Subject: [Histonet] Question about Lyme Disease Message-ID: Have a patient request for testing but all we have is FFPE blocks. Does anyone know anyone who tests on FFPE? Thanks!! Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From Richard.Cartun <@t> hhchealth.org Fri Oct 25 13:25:01 2013 From: Richard.Cartun <@t> hhchealth.org (Cartun, Richard) Date: Fri Oct 25 13:25:07 2013 Subject: [Histonet] RE: Question about Lyme Disease In-Reply-To: References: Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E0188A8BB@HHCEXCHMB05.hhcsystem.org> In my experience, it's not worth looking at tissue for the spirochete using histochemical stains or IHC. There are labs that do PCR off FFPE-tissue. Has serology been done? Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax richard.cartun@hhchealth.org ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Weems, Joyce K. [Joyce.Weems@emoryhealthcare.org] Sent: Friday, October 25, 2013 2:07 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Question about Lyme Disease Have a patient request for testing but all we have is FFPE blocks. Does anyone know anyone who tests on FFPE? Thanks!! Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From escott8 <@t> comcast.net Fri Oct 25 16:51:48 2013 From: escott8 <@t> comcast.net (escott8@comcast.net) Date: Fri Oct 25 16:52:22 2013 Subject: [Histonet] RE(4); 98 Message-ID: <1061160911.7185925.1382737908065.JavaMail.root@comcast.net> referral program http://buckroyd.com/wp-content/SALEBESTSELLER.php Sent: 10/25/2013 2:51:30 PM >From escott8 From escott8 <@t> comcast.net Fri Oct 25 22:57:16 2013 From: escott8 <@t> comcast.net (escott8@comcast.net) Date: Fri Oct 25 22:57:50 2013 Subject: [Histonet] re: 99 Message-ID: <1841970417.7316240.1382759836852.JavaMail.root@comcast.net> referral link http://topproline.com/images/SALEBESTSELLER.php Sent: 10/25/2013 8:56:57 PM >From escott8 From Gary_Steinke <@t> vwr.com Sat Oct 26 12:05:22 2013 From: Gary_Steinke <@t> vwr.com (Gary_Steinke@vwr.com) Date: Sat Oct 26 12:05:46 2013 Subject: [Histonet] Gary Steinke is out of the office Message-ID: I will be out of the office starting 10/26/2013 and will not return until 02/28/2014. Gary is no longer with VWR. Please contact Customer Care at healthcareservice@vwr.com or by phone at 877-881-1192. Thank you! From adesupo2002 <@t> hotmail.com Sun Oct 27 20:26:30 2013 From: adesupo2002 <@t> hotmail.com (ADESUPO ADESUYI) Date: Sun Oct 27 20:26:37 2013 Subject: [Histonet] Bechmarks for Inadequate Specimens Message-ID: Please I will appreciate it, if you guys here in histo-land could tell me if there were any national standards or benchmarks for inadequate specimen rates concerning the submission of tissue to Pathology for grossing and analysis. Thanks, Adesupo Adesuyi From Nancy_Schmitt <@t> pa-ucl.com Mon Oct 28 08:34:55 2013 From: Nancy_Schmitt <@t> pa-ucl.com (Nancy Schmitt) Date: Mon Oct 28 08:35:00 2013 Subject: [Histonet] Microtome Blade safety, in or out when not in use? In-Reply-To: <20131025170407.18BA91AA036@mail.pa-ucl.com> References: <20131025170407.18BA91AA036@mail.pa-ucl.com> Message-ID: <906B4DA90ED1DB4DB6C7E94D7CEE6C368150E62F@PEITHA.wad.pa-ucl.com> Hi Leah- We leave the blade in behind the guard and we also lock the wheel when we leave the microtome. Nancy Histology Coordinator Dubuque, IA _______________________________________________________ Message: 8 Date: Fri, 25 Oct 2013 12:33:43 +1100 From: Leah Simmons Subject: [Histonet] Microtome Blade safety, in or out when not in use? To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hello all :-) I am doing a quick microtome blade safety survey, When you finish work, do you leave your blade in the microtome behind the blade guard or do you take it out? If you take it out and it is a new blade or a blade still useful for trimming where do you store it? Thank you for your feedback, I really appreciate it. Regards Leah Simmons NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From mkapnge <@t> yahoo.com Mon Oct 28 12:03:36 2013 From: mkapnge <@t> yahoo.com (Mwanza Kapnge) Date: Mon Oct 28 12:03:47 2013 Subject: [Histonet] RE: Looking for HTL or HT position for US Work experience Message-ID: <1382979816.67533.YahooMailNeo@web140803.mail.bf1.yahoo.com> Dear Histonet Job Coordinator, I am HTL trained and worked in United Kingdom.? Now that I am in United States I would like to continue to practice.? The ASCP after evaluating my education and training has asked me to look for?a lab in United States for my work experience here so that I can sit for ASCP exam for certification. I would be very pleased if I can find a place. I have?10 years?work?experience in histology lab. Sincerely, ? ? Mwanz kapnge From Rhonda.Gregoire <@t> gov.mb.ca Mon Oct 28 12:19:47 2013 From: Rhonda.Gregoire <@t> gov.mb.ca (Gregoire, Rhonda (MAFRI)) Date: Mon Oct 28 12:20:10 2013 Subject: [Histonet] LUXOL FAST BLUE Message-ID: Hi Thomas, Would you be able to share your Luxol Fast Blue procedure, I would like to try differentiating with the hydoquinone solution. We are cutting thicker sections (15um) in our lab so would have to adjust the timing. Is it still possible to over-differentiate using this method? Thanks Rhonda Gregoire, MLT Supervisor, Clinical Pathology Veterinary Diagnostic Services Manitoba Agriculture, Food and Rural Development 545 University Crescent Winnipeg, MB R3T 5S6 phone 204-945-7641 fax 204-945-7646 email Rhonda.Gregoire@gov.mb.ca From brett_connolly <@t> merck.com Mon Oct 28 12:56:12 2013 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Mon Oct 28 12:56:20 2013 Subject: [Histonet] looking for sheep lung blocks Message-ID: Hi all, Does anyone know where I could get some FFPE blocks of normal sheep lung? If so, please contact me via e-mail directly. Thanks, Brett Brett M. Connolly, Ph.D. Principal Scientist, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From jeanross <@t> udel.edu Mon Oct 28 13:25:04 2013 From: jeanross <@t> udel.edu (Jean Ross) Date: Mon Oct 28 13:25:07 2013 Subject: [Histonet] cryostat service Message-ID: Hi everyone, I am interested in finding out if there is anyone in or close to Delaware that services and repairs cryostats. Any recommendations would be much appreciated. Thanks, Jean -- Jean Ross Delaware Biotechnology Institute, BioImaging Center University of Delaware 15 Innovation Way Suite 117 Newark, DE 19711 From trathborne <@t> somerset-healthcare.com Mon Oct 28 13:29:46 2013 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Mon Oct 28 13:30:08 2013 Subject: [Histonet] cryostat service In-Reply-To: References: Message-ID: <3AD061FE740D464FAC7BF6B5CFB75707A8B08929@smcmail02.somerset-healthcare.com> Avantik is located in NJ and services cryostats in addition to microtomes, processors and stainers. www.avantik-us.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jean Ross Sent: Monday, October 28, 2013 2:25 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cryostat service Hi everyone, I am interested in finding out if there is anyone in or close to Delaware that services and repairs cryostats. Any recommendations would be much appreciated. Thanks, Jean -- Jean Ross Delaware Biotechnology Institute, BioImaging Center University of Delaware 15 Innovation Way Suite 117 Newark, DE 19711 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From barbara.tibbs <@t> accuratediagnosticlabs.com Mon Oct 28 13:31:19 2013 From: barbara.tibbs <@t> accuratediagnosticlabs.com (Barbara Tibbs) Date: Mon Oct 28 13:31:31 2013 Subject: [Histonet] cryostat service In-Reply-To: References: Message-ID: Hello, BelAir is an excellent repair service company located in NJ. They will come to Delaware for repairs. Look them up online for the contact information. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.tibbs@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Jean Ross Sent: Monday, October 28, 2013 4:25 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cryostat service Hi everyone, I am interested in finding out if there is anyone in or close to Delaware that services and repairs cryostats. Any recommendations would be much appreciated. Thanks, Jean -- Jean Ross Delaware Biotechnology Institute, BioImaging Center University of Delaware 15 Innovation Way Suite 117 Newark, DE 19711 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wbenton <@t> cua.md Mon Oct 28 13:34:38 2013 From: wbenton <@t> cua.md (Walter Benton) Date: Mon Oct 28 13:35:57 2013 Subject: [Histonet] cryostat service In-Reply-To: <3AD061FE740D464FAC7BF6B5CFB75707A8B08929@smcmail02.somerset-healthcare.com> References: , <3AD061FE740D464FAC7BF6B5CFB75707A8B08929@smcmail02.somerset-healthcare.com> Message-ID: <0B8979A204680A42B93A52B486088CD93931ABEF9D@CUAEXH1.GCU-MD.local> Bel Air Instruments and Dolbey Jamison Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) ChesapeakeUrology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni [trathborne@somerset-healthcare.com] Sent: Monday, October 28, 2013 2:29 PM To: 'Jean Ross'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] cryostat service Avantik is located in NJ and services cryostats in addition to microtomes, processors and stainers. www.avantik-us.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jean Ross Sent: Monday, October 28, 2013 2:25 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cryostat service Hi everyone, I am interested in finding out if there is anyone in or close to Delaware that services and repairs cryostats. Any recommendations would be much appreciated. Thanks, Jean -- Jean Ross Delaware Biotechnology Institute, BioImaging Center University of Delaware 15 Innovation Way Suite 117 Newark, DE 19711 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From craigak12 <@t> gmail.com Mon Oct 28 16:17:55 2013 From: craigak12 <@t> gmail.com (Jb) Date: Mon Oct 28 16:18:02 2013 Subject: [Histonet] Thermo equipment: Message-ID: Does anyone have experience/input on Thermo Scientific equipment? Ex the slide labeling system (slideMate), processor, cassette printing system (printMate). We are considering purchasing this equipment and would like to hear as much input as possible. Good/bad, other suggestions, etc. Thank you- Sent from my iPhone From badzrosari <@t> yahoo.com Mon Oct 28 22:41:34 2013 From: badzrosari <@t> yahoo.com (Bernadette del Rosario) Date: Mon Oct 28 22:41:39 2013 Subject: [Histonet] Lymphnode retreival solution Message-ID: <1383018094.78802.YahooMailNeo@web121503.mail.ne1.yahoo.com> Hello there.I wonder how long you fix the fats in the Penfix solution?How about in GEWF solution?? From Colleen_Herring <@t> bshsi.org Tue Oct 29 05:23:58 2013 From: Colleen_Herring <@t> bshsi.org (Herring, Colleen) Date: Tue Oct 29 05:24:08 2013 Subject: [Histonet] Validation Message-ID: <3AE5857C7EC73F4E983F39C97DF811C0034565@EDC-EXMB2-01.ads.bshsi.com> We are getting the new Ventana Nexus in next month and I would like any input. After I optimize all the stains what is the amount of positive controls do I run on each to validate? Thanks in advance ________________________________________________________________________________________________________ The information in this communication is intended to be confidential to the Individual(s) and/or Entity to whom it is addressed. It may contain information of a Privileged and/or Confidential nature, which is subject to Federal and/or State privacy regulations. In the event that you are not the intended recipient or the agent of the intended recipient, do not copy or use the information contained within this communication, or allow it to be read, copied or utilized in any manner, by any other person(s). Should this communication be received in error, please notify the sender immediately either by response e-mail or by phone, and permanently delete the original e-mail, attachment(s), and any copies. ________________________________________________________________________________________________________ From Beth.Fye <@t> HCAhealthcare.com Tue Oct 29 08:09:54 2013 From: Beth.Fye <@t> HCAhealthcare.com (Beth.Fye@HCAhealthcare.com) Date: Tue Oct 29 08:10:53 2013 Subject: [Histonet] RE: Breast Core needle biopsy procedure question. Message-ID: <938F8EC5A524D34EB5796E23E52781D361CA3D1C4D@NADCWPMSGCMS05.hca.corpad.net> I hope this helps. We had problems here before implementing this. Here is what we have listed for breast cores in our microtomy procedure: Breast Core Biopsy Breast core biopsy containing calcium is designated as CA ++ on the side of the block. This is normally the "A" block of a multiple block breast core case. The following protocol should be followed when cutting breast core CA ++ block. Trim the block sparingly RIBBON #1 - Take three superficial sections; one from the beginning, one from the middle, and one from the end of the ribbon. Label slides S1, S2, S3. These will be stained H&E. Cut three levels and three spare slides as follows: RIBBON #2 - One Spare Slide RIBBON #3 - LEVEL 1 RIBBON #4 - One Spare Slide RIBBON #5 - LEVEL 2 RIBBON #6 - One Spare Slide RIBBON #7 - LEVEL 3 Label spare slides 1, 2, 3 Save spare slides for further testing. All other slides should be stained for Hematoxylin & Eosin. Beth A. Fye, CT (ASCP) Pathology Technical Manager HCA Richmond Hospital Laboratories office: (804) 228-6564 fax: (877)699-3895 From PAMarcum <@t> uams.edu Tue Oct 29 08:13:53 2013 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Tue Oct 29 08:13:59 2013 Subject: [Histonet] Thermo equipment: In-Reply-To: References: Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA32D315ED72@Mail2Node2.ad.uams.edu> We have had the Thermo system with the slide writers and cassette writers for a little over two and a half years. We did have issues when we got the system as it was new to the market and had some bugs. Thermo fixed the problems and Thermo stood by us until we got everything we needed. They did it very quickly to make us a very happy group of Histologists and the Gross Room techs. We now have two other labs who bought the system a year after we did and all the bugs were gone. They have had no problems and love the systems as much as we do. We do decal nightly on bone marrows and have never had a cassette label fade or come off in the solution. It was an absolute necessity for us to know the information did not fade and the cassette writer was the only I was comfortable would hold up for this as it literally "burns" the number into the plastic. Pam Marcum UAMS -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jb Sent: Monday, October 28, 2013 4:18 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Thermo equipment: Does anyone have experience/input on Thermo Scientific equipment? Ex the slide labeling system (slideMate), processor, cassette printing system (printMate). We are considering purchasing this equipment and would like to hear as much input as possible. Good/bad, other suggestions, etc. Thank you- Sent from my iPhone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From llang <@t> aipathology.com Tue Oct 29 08:21:37 2013 From: llang <@t> aipathology.com (LeAnn Lang) Date: Tue Oct 29 08:21:45 2013 Subject: [Histonet] 99000 - Can we or can we not bill for it Message-ID: <704247D5A09D004C9E6B115138D1703AB2B952@hpserv001.aipathology.local> Can someone clarify for me if a pathology lab can bill for 99000? I am getting contradicting information online. CAP says: Reprinted from December 2000 CAP TODAY Q: My practice is interested in using code 99000, Handling and/or conveyance of specimen for transfer from the physician's office to a laboratory. What is the proper application of this code? A: Code 99000 can be used when the physician incurs costs for handling or transporting a specimen to the laboratory, or both. The American Medical Association has clarified that 99000 can be used to capture the work involved in preparing a specimen for transportation to the laboratory, or in transporting the specimen. Preparation for transportation is reported regardless of whether the specimen is delivered by the physician office or a courier (CPT Assistant, October 1999). Preparation may include specimen centrifugation, separating serum, labeling tubes and envelopes, packing the specimen before transport, completing laboratory forms, and supplying necessary insurance information and other documents. Medicare and most private insurers, however, do not recognize this code. Another group I talked with says they are getting paid for the services due to costs of supplies and courier charges. Nowhere does it specifically state that a pathologist can bill for the specimen handling. Anyone else billing with this code or have information to help us out! Thank you, LeAnn <>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<> LeAnn Lang Associates in Pathology Practice Administrator Phone: 715-847-0075 (ext 50259) llang@aipathology.com From billodonnell <@t> catholichealth.net Tue Oct 29 08:42:10 2013 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Tue Oct 29 08:42:26 2013 Subject: [Histonet] Thermo equipment: In-Reply-To: <41D3A1AF6FEF0643BDC89E0516A6EA32D315ED72@Mail2Node2.ad.uams.edu> References: <41D3A1AF6FEF0643BDC89E0516A6EA32D315ED72@Mail2Node2.ad.uams.edu> Message-ID: I ewcho Pam's message. Buggy at first, but stayed buggy for a long time w the cassette printer. They have replaced the heads on cassette writer a number of times, but we now have one that works great and we are happy with them. I have no experience w their processor. - Bill -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marcum, Pamela A Sent: Tuesday, October 29, 2013 8:14 AM To: 'Jb'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Thermo equipment: We have had the Thermo system with the slide writers and cassette writers for a little over two and a half years. We did have issues when we got the system as it was new to the market and had some bugs. Thermo fixed the problems and Thermo stood by us until we got everything we needed. They did it very quickly to make us a very happy group of Histologists and the Gross Room techs. We now have two other labs who bought the system a year after we did and all the bugs were gone. They have had no problems and love the systems as much as we do. We do decal nightly on bone marrows and have never had a cassette label fade or come off in the solution. It was an absolute necessity for us to know the information did not fade and the cassette writer was the only I was comfortable would hold up for this as it literally "burns" the number into the plastic. Pam Marcum UAMS -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jb Sent: Monday, October 28, 2013 4:18 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Thermo equipment: Does anyone have experience/input on Thermo Scientific equipment? Ex the slide labeling system (slideMate), processor, cassette printing system (printMate). We are considering purchasing this equipment and would like to hear as much input as possible. Good/bad, other suggestions, etc. Thank you- Sent from my iPhone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. From PAMarcum <@t> uams.edu Tue Oct 29 09:06:58 2013 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Tue Oct 29 09:07:08 2013 Subject: [Histonet] Thermo equipment: In-Reply-To: References: <41D3A1AF6FEF0643BDC89E0516A6EA32D315ED72@Mail2Node2.ad.uams.edu> Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA32D315EDDA@Mail2Node2.ad.uams.edu> We also have 4 Excelsiors in our area. We replaced the first VIP processor 4 years ago when it finally gave up on us. I will say the VIP was over twenty years old when it died. We then purchased the second Excelsior a year later so we have a dedicated biopsy unit. Tragedy hit again as the last two VIPS (same age) died and we needed to have consistency in processing and training. We have had very good success with the processors and any issues at all with service have been taken care of quickly. They are great processors and the gently agitation helped us shorten some of our processes and the pathologist are much happier. Pam Marcum UAMS -----Original Message----- From: O'Donnell, Bill [mailto:billodonnell@catholichealth.net] Sent: Tuesday, October 29, 2013 8:42 AM To: Marcum, Pamela A; 'Jb'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Thermo equipment: I ewcho Pam's message. Buggy at first, but stayed buggy for a long time w the cassette printer. They have replaced the heads on cassette writer a number of times, but we now have one that works great and we are happy with them. I have no experience w their processor. - Bill -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marcum, Pamela A Sent: Tuesday, October 29, 2013 8:14 AM To: 'Jb'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Thermo equipment: We have had the Thermo system with the slide writers and cassette writers for a little over two and a half years. We did have issues when we got the system as it was new to the market and had some bugs. Thermo fixed the problems and Thermo stood by us until we got everything we needed. They did it very quickly to make us a very happy group of Histologists and the Gross Room techs. We now have two other labs who bought the system a year after we did and all the bugs were gone. They have had no problems and love the systems as much as we do. We do decal nightly on bone marrows and have never had a cassette label fade or come off in the solution. It was an absolute necessity for us to know the information did not fade and the cassette writer was the only I was comfortable would hold up for this as it literally "burns" the number into the plastic. Pam Marcum UAMS -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jb Sent: Monday, October 28, 2013 4:18 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Thermo equipment: Does anyone have experience/input on Thermo Scientific equipment? Ex the slide labeling system (slideMate), processor, cassette printing system (printMate). We are considering purchasing this equipment and would like to hear as much input as possible. Good/bad, other suggestions, etc. Thank you- Sent from my iPhone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. From GKeyser <@t> uwhealth.org Tue Oct 29 13:47:07 2013 From: GKeyser <@t> uwhealth.org (Keyser Gerald T) Date: Tue Oct 29 13:47:12 2013 Subject: [Histonet] Kisser's Mounting Media (Glycerol jelly) Message-ID: <5226C88C65EBFF4BAD552D68DC6E8FFE01D1CD@UWHC-MBX12.uwhis.hosp.wisc.edu> I'm toying with making my own aqueous mounting media. Although I will not likely use this in actual cases, it would still be nice. Aqueous mounting media is kind of expensive. But, there are some questions that need to be answered. The Slides need to be kept for 10 years. - Will the adhesive yellow with time? - Will the adhesive continue to have complete coverage over time. Over drying? Cracking? - Since the mounting media is made from glycerin, gelatin, and water, will microbial invasion of the slides be a problem? I can add a anti-microbial agent to the recipe. Anyone have long term experience with this recipe? From barryrittman <@t> gmail.com Tue Oct 29 14:40:48 2013 From: barryrittman <@t> gmail.com (Barry Rittman) Date: Tue Oct 29 14:40:52 2013 Subject: [Histonet] Kisser's Mounting Media (Glycerol jelly) In-Reply-To: <5226C88C65EBFF4BAD552D68DC6E8FFE01D1CD@UWHC-MBX12.uwhis.hosp.wisc.edu> References: <5226C88C65EBFF4BAD552D68DC6E8FFE01D1CD@UWHC-MBX12.uwhis.hosp.wisc.edu> Message-ID: You can use thymol or merthiolate to prevent mold. You can always seal the edges of the coverslip. Barry On Tue, Oct 29, 2013 at 1:47 PM, Keyser Gerald T wrote: > I'm toying with making my own aqueous mounting media. Although I will not > likely use this in actual cases, it would still be nice. Aqueous mounting > media is kind of expensive. But, there are some questions that need to be > answered. > > The Slides need to be kept for 10 years. > > - Will the adhesive yellow with time? > > - Will the adhesive continue to have complete coverage over time. Over > drying? Cracking? > > - Since the mounting media is made from glycerin, gelatin, and water, will > microbial invasion of the slides be a problem? I can add a anti-microbial > agent to the recipe. > > Anyone have long term experience with this recipe? > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Tony.Reilly <@t> health.qld.gov.au Tue Oct 29 18:01:02 2013 From: Tony.Reilly <@t> health.qld.gov.au (Tony Reilly) Date: Tue Oct 29 18:01:24 2013 Subject: [Histonet] Kisser's Mounting Media (Glycerol jelly) In-Reply-To: References: <5226C88C65EBFF4BAD552D68DC6E8FFE01D1CD@UWHC-MBX12.uwhis.hosp.wisc.edu> Message-ID: Hello In the dark old days we would seal the edges of the coverslip with nail polish. Regards Tony Tony Reilly B.App.Sc. , M.Sc. Chief Scientist, Anatomical Pathology Pathology Queensland-PA Laboratory ________________________________________________ Health Services Support Agency | Department of Health Level 1, Building 15,Princess Alexandra Hospital Ipswich Road,WOOLLOONGABBA Qld 4102 Ph: 07 3176 2412 Mob: 0402 139411 Fax: 07 3176 2930 Email: tony.reilly@health.qld.gov.au Web: www.health.qld.gov.au/qhcss/ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barry Rittman Sent: Wednesday, 30 October 2013 5:41 AM To: Tony Reilly Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Kisser's Mounting Media (Glycerol jelly) You can use thymol or merthiolate to prevent mold. You can always seal the edges of the coverslip. Barry On Tue, Oct 29, 2013 at 1:47 PM, Keyser Gerald T wrote: > I'm toying with making my own aqueous mounting media. Although I will not > likely use this in actual cases, it would still be nice. Aqueous mounting > media is kind of expensive. But, there are some questions that need to be > answered. > > The Slides need to be kept for 10 years. > > - Will the adhesive yellow with time? > > - Will the adhesive continue to have complete coverage over time. Over > drying? Cracking? > > - Since the mounting media is made from glycerin, gelatin, and water, will > microbial invasion of the slides be a problem? I can add a anti-microbial > agent to the recipe. > > Anyone have long term experience with this recipe? > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ********************************************************************************** From barryrittman <@t> gmail.com Tue Oct 29 18:26:39 2013 From: barryrittman <@t> gmail.com (Barry Rittman) Date: Tue Oct 29 18:26:46 2013 Subject: [Histonet] Kisser's Mounting Media (Glycerol jelly) In-Reply-To: References: <5226C88C65EBFF4BAD552D68DC6E8FFE01D1CD@UWHC-MBX12.uwhis.hosp.wisc.edu> Message-ID: Nail varnish works well Alternatives include using pink dental wax that melts at relatively low temperature or a mixture of Vaseline/lanoline/paraffin wax (equal parts mixed with heat). Both seal well and are impervious to aqueous solutions. Barry On Tue, Oct 29, 2013 at 6:01 PM, Tony Reilly wrote: > Hello > > In the dark old days we would seal the edges of the coverslip with nail > polish. > > Regards > Tony > > > > Tony Reilly B.App.Sc. , M.Sc. > > Chief Scientist, Anatomical Pathology > > Pathology Queensland-PA Laboratory > > ________________________________________________ > Health Services Support Agency | Department of Health > > > > Level 1, Building 15,Princess Alexandra Hospital > > Ipswich Road,WOOLLOONGABBA Qld 4102 > Ph: 07 3176 2412 > Mob: 0402 139411 > > Fax: 07 3176 2930 > > Email: tony.reilly@health.qld.gov.au > > Web: www.health.qld.gov.au/qhcss/ > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barry Rittman > Sent: Wednesday, 30 October 2013 5:41 AM > To: Tony Reilly > Cc: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Kisser's Mounting Media (Glycerol jelly) > > You can use thymol or merthiolate to prevent mold. > You can always seal the edges of the coverslip. > Barry > > > > On Tue, Oct 29, 2013 at 1:47 PM, Keyser Gerald T >wrote: > > > I'm toying with making my own aqueous mounting media. Although I will not > > likely use this in actual cases, it would still be nice. Aqueous mounting > > media is kind of expensive. But, there are some questions that need to be > > answered. > > > > The Slides need to be kept for 10 years. > > > > - Will the adhesive yellow with time? > > > > - Will the adhesive continue to have complete coverage over time. Over > > drying? Cracking? > > > > - Since the mounting media is made from glycerin, gelatin, and water, > will > > microbial invasion of the slides be a problem? I can add a anti-microbial > > agent to the recipe. > > > > Anyone have long term experience with this recipe? > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ******************************************************************************** > This email, including any attachments sent with it, is confidential and > for the sole use of the intended recipient(s). This confidentiality is not > waived or lost, if you receive it and you are not the intended > recipient(s), or if it is transmitted/received in error. > Any unauthorised use, alteration, disclosure, distribution or review of > this email is strictly prohibited. The information contained in this > email, including any attachment sent with it, may be subject to a statutory > duty of confidentiality if it relates to health service matters. > If you are not the intended recipient(s), or if you have received this > email in error, you are asked to immediately notify the sender by telephone > collect on Australia +61 1800 198 175 or by return email. You should > also delete this email, and any copies, from your computer system network > and destroy any hard copies produced. > If not an intended recipient of this email, you must not copy, distribute > or take any action(s) that relies on it; any form of disclosure, > modification, distribution and/or publication of this email is also > prohibited. > Although Queensland Health takes all reasonable steps to ensure this email > does not contain malicious software, Queensland Health does not accept > responsibility for the consequences if any person's computer inadvertently > suffers any disruption to services, loss of information, harm or is > infected with a virus, other malicious computer programme or code that may > occur as a consequence of receiving this email. > Unless stated otherwise, this email represents only the views of the > sender and not the views of the Queensland Government. > > ********************************************************************************** > > From escott8 <@t> comcast.net Wed Oct 30 09:16:17 2013 From: escott8 <@t> comcast.net (escott8@comcast.net) Date: Wed Oct 30 09:16:36 2013 Subject: [Histonet] RE; 6 Message-ID: <1188707850.10382332.1383142577992.JavaMail.root@comcast.net> referral program http://no-faxing-payday-loan.com/personalloan/TATI.php Sent: 10/30/2013 7:16:12 AM >From escott8 From ihcs <@t> smithsys.net Tue Oct 29 21:32:50 2013 From: ihcs <@t> smithsys.net (ihcs-wojcieszyn) Date: Wed Oct 30 09:31:48 2013 Subject: [Histonet] Did you see the listings concerning payday loans????????? Message-ID: <92553F6A-410B-11E3-870A-003065A5E3AC@smithsys.net> Why this even get on your system??????????? From histo <@t> pathlab.us Wed Oct 30 10:59:52 2013 From: histo <@t> pathlab.us (Histology) Date: Wed Oct 30 10:59:58 2013 Subject: [Histonet] Ventana vs. Leica IHC stainers Message-ID: <9C0B151B30AC5F4ABFD1B69D915F84CF33EF2F@dc01.DominionPath.local> Hi all, I'm trying to decide between a Ventana Ultra and a Leica Bond IHC stainer. Has anyone tried both of these? If so, care to share your thoughts on both? Particularly interested in hazardous waste, tissue washing, and DIF procedures. Thanks to all in advance!! Mehndi Helgren Dominion Pathology Laboratories 733 Boush St. Suite 200 Norfolk, VA 23510 757-664-7901 histo@pathlab.us From joelleweaver <@t> hotmail.com Wed Oct 30 14:19:57 2013 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Wed Oct 30 14:20:03 2013 Subject: [Histonet] Did you see the listings concerning payday loans????????? In-Reply-To: <92553F6A-410B-11E3-870A-003065A5E3AC@smithsys.net> References: <92553F6A-410B-11E3-870A-003065A5E3AC@smithsys.net> Message-ID: I get that post & link about once per week via the histonet Joelle Weaver MAOM, HTL (ASCP) QIHC > Date: Tue, 29 Oct 2013 20:32:50 -0600 > From: ihcs@smithsys.net > To: Histonet@lists.utsouthwestern.edu > CC: > Subject: [Histonet] Did you see the listings concerning payday loans????????? > > > Why this even get on your system??????????? > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pscott <@t> scigene.com Wed Oct 30 15:26:55 2013 From: pscott <@t> scigene.com (Paul Scott) Date: Wed Oct 30 15:27:17 2013 Subject: [Histonet] Re: Kisser's Mounting Media (Glycerol jelly) Message-ID: <002601ced5ae$6041bdb0$20c53910$@com> Hi Histonetters, My company makes a temporary coverslip sealant called CytoBond that was designed as a superior replacement for rubber cement in FISH but we are getting increasing interest for IHC applications. The seal withstands high temperatures without requiring humidification and freezing, yet is easily removed in one piece. CytoBond has been on the market a couple of years with an ever increasing customer base. Full product information available here: http://www.scigene.com/documents/M225_CytoBond.pdf and here http://www.scigene.com/details.php?pid=1216 I can send FREE samples to interested parties, you can contact me using the information below. Many thanks, paul Paul Scott SciGene 470 Lakeside Drive, Ste F, Sunnyvale, CA 94085-4720 408-733-7337 x 305 pscott@scigene.com Automating FISH and CMA Workflows www.SciGene.com From stephen.jew <@t> sydney.edu.au Wed Oct 30 16:40:57 2013 From: stephen.jew <@t> sydney.edu.au (Stephen KumJew) Date: Wed Oct 30 16:41:56 2013 Subject: [Histonet] esterase Message-ID: I was wondering if anyone knew of an esterase stain that doesn't stain the neuromuscular junction too dark. Have tried a modified technique by BJ Davis which uses naphthyl acetate, acetone 0.2M sodium phosphate and azotised pararosaniline but the staining was a dark red/black. Tried reducing times and temperature and reducing the pararosaniline but no change. Thanks Stephen STEPHEN KUM JEW | Senior Technical Officer Discipline of Pathology School of Medical Sciences | Blackburn Building | D06, The University of Sydney | NSW | 2006 T +61 2 9036 9027| F +61 2 9351 3429 E stephen.jew@sydney.edu.au | W http://sydney.edu.au/medicine/pathology/ This email plus any attachments to it are confidential. Any unauthorised use is strictly prohibited. If you receive this email in error, please delete it and any attachments. Please consider the environment before printing this email. From Joyce.Weems <@t> emoryhealthcare.org Wed Oct 30 16:57:39 2013 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Wed Oct 30 16:58:00 2013 Subject: [Histonet] Kappa and Lambda ISH Message-ID: For those of you doing these successfully on bone marrows - aspirate and biopsy, would you please contact me off the list? We're having issues with the tissues... Thanks for your help! Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From Tony.Reilly <@t> health.qld.gov.au Wed Oct 30 18:16:43 2013 From: Tony.Reilly <@t> health.qld.gov.au (Tony Reilly) Date: Wed Oct 30 18:17:52 2013 Subject: [Histonet] RE: Ventana vs. Leica IHC stainers In-Reply-To: <9C0B151B30AC5F4ABFD1B69D915F84CF33EF2F@dc01.DominionPath.local> References: <9C0B151B30AC5F4ABFD1B69D915F84CF33EF2F@dc01.DominionPath.local> Message-ID: Hi Mehndi I have worked in labs that have used either the Ventana or the Bond. I have also been involved in evaluating both side by side in my current lab. As far as IHC is involved the Bond staining is in some case stronger but no more sensitive so it depends if you want punchy staining or more delicate staining. When asked our pathologists did not have a preference and were happy to get either instrument. Not sure about the Bond now but when we evaluated in 2010 their CISH was not up and working. We are very happy with the SISH on the Ventana. If you only want to do IHC either will do the job and you should make your judgement on price and after sales customer service. They will both want to charge you for their proprietary reagents based on your workload. If you have a large workload that is fine, but if you have a relatively small workload you may be better off looking at some of the open IHC systems available. Regards Tony Tony Reilly B.App.Sc. , M.Sc. Chief Scientist, Anatomical Pathology Pathology Queensland-PA Laboratory ________________________________________________ Health Services Support Agency | Department of Health Level 1, Building 15,Princess Alexandra Hospital Ipswich Road,WOOLLOONGABBA Qld 4102 Ph: 07 3176 2412 Mob: 0402 139411 Fax: 07 3176 2930 Email: tony.reilly@health.qld.gov.au Web: www.health.qld.gov.au/qhcss/ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Histology Sent: Thursday, 31 October 2013 2:00 AM To: Tony Reilly; histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana vs. Leica IHC stainers Hi all, I'm trying to decide between a Ventana Ultra and a Leica Bond IHC stainer. Has anyone tried both of these? If so, care to share your thoughts on both? Particularly interested in hazardous waste, tissue washing, and DIF procedures. Thanks to all in advance!! Mehndi Helgren Dominion Pathology Laboratories 733 Boush St. Suite 200 Norfolk, VA 23510 757-664-7901 histo@pathlab.us _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. 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Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ********************************************************************************** From benoit.delatour <@t> upmc.fr Thu Oct 31 02:57:12 2013 From: benoit.delatour <@t> upmc.fr (=?UTF-8?B?QmVub8OudCBEZWxhdG91cg==?=) Date: Thu Oct 31 02:55:07 2013 Subject: [Histonet] Remove OCT from frozen tissue block Message-ID: <52720D58.8010301@upmc.fr> Dear histoneters, We got mice brains that were initially sucrose-cryoprotected and then embedded in OCT - flash frozen and stored at -80?C. These tissues were initially prepared for subsequent cryostat sectioning but due to technical considerations we need to cut them using a sliding microtome to get thick (40?m) floating sections. We therefore would like to remove OCT before cutting. As OCT is water soluble it is expected that placing the OCT blocks in buffer would help removing the embedding media but thawing the tissue and then re-freezing it on the microtome stage might not be the best way to proceed (expected formation of ice crystals with known associated histological artefacts)... We would appreciate any alternative solutions. Thanks! Beno?t From escott8 <@t> comcast.net Thu Oct 31 03:21:45 2013 From: escott8 <@t> comcast.net (escott8@comcast.net) Date: Thu Oct 31 03:22:17 2013 Subject: [Histonet] re(2); 33 Message-ID: <1193952506.13210.1383207705065.JavaMail.root@comcast.net> referral program http://homegate-ng.com/Scripts/TATI.php Sent: 10/31/2013 1:21:37 AM >From escott8 From patpxs <@t> gmail.com Thu Oct 31 06:14:45 2013 From: patpxs <@t> gmail.com (Paula Sicurello) Date: Thu Oct 31 06:14:53 2013 Subject: [Histonet] Remove OCT from frozen tissue block In-Reply-To: <52720D58.8010301@upmc.fr> References: <52720D58.8010301@upmc.fr> Message-ID: Hi Benoit, You can just let the OCT thaw at room temperature then blot away the melted OCT. To speed up the thawing process carefully cut away as much of the OCT as you can with a scalpel or razor blade and then let it thaw. I hope this information is helpful. Paula On Thu, Oct 31, 2013 at 3:57 AM, Beno?t Delatour wrote: > Dear histoneters, > We got mice brains that were initially sucrose-cryoprotected and then > embedded in OCT - flash frozen and stored at -80?C. These tissues were > initially prepared for subsequent cryostat sectioning but due to technical > considerations we need to cut them using a sliding microtome to get thick > (40?m) floating sections. We therefore would like to remove OCT before > cutting. As OCT is water soluble it is expected that placing the OCT blocks > in buffer would help removing the embedding media but thawing the tissue > and then re-freezing it on the microtome stage might not be the best way to > proceed (expected formation of ice crystals with known associated > histological artefacts)... > We would appreciate any alternative solutions. Thanks! > Beno?t > > > ______________________________**_________________ > Histonet mailing list > Histonet@lists.utsouthwestern.**edu > http://lists.utsouthwestern.**edu/mailman/listinfo/histonet > -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 HIPAA Privacy Notification: This message and any accompanying documents are covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, and contain information intended for the specific individual (s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying or the taking of any action based on the contents of this information is strictly prohibited . If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From ibernard <@t> uab.edu Thu Oct 31 06:47:30 2013 From: ibernard <@t> uab.edu (Ian R Bernard) Date: Thu Oct 31 06:47:38 2013 Subject: [Histonet] Ideal Workflow Lab Design for Histo/Cyto lab Processes Message-ID: Histonetters, I'm looking for information or references concerning the ideal workflow design for a Histo/Cyto lab services. Need to consider ergonomics, work space, equipment, safety, etc. I know there are info. Just need source and experts to chime in. Journal of Histotechnology Advance article etc. Please reply to my work email as well. Ian Bernard From mward <@t> wakehealth.edu Thu Oct 31 07:18:36 2013 From: mward <@t> wakehealth.edu (Martha Ward-Pathology) Date: Thu Oct 31 07:18:44 2013 Subject: [Histonet] cell block staining issues In-Reply-To: <52720D58.8010301@upmc.fr> References: <52720D58.8010301@upmc.fr> Message-ID: We have something of a mystery here and I am hoping someone can help. My cytopath docs are saying that our B72.3 (TAG-72) antibody is not working well in cell blocks created from fluids (plural), etc. and that this is a fairly recent development (over the past 5-6 weeks or so). The surgical cases we have stained look great as does our control, which is placed on each slide. We have not changed lot numbers of antibody or made any up recently and the antibody is not expired, or even close. The only thing we can find that is different is that cytology has been rinsing the needles out with a product called Cytolyt, instead of saline and they started this around the first of August. Does anyone know if this product could cause any interference with our staining? The docs just say that the tumor cells are not staining and we are sort of at a loss here. Thanks in advance for your help. Martha Ward, MT (ASCP) QIHC Manager [Wake Forest Baptist Health] Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mward@wakehealth.edu From TMcNemar <@t> lmhealth.org Thu Oct 31 08:16:42 2013 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Thu Oct 31 08:16:52 2013 Subject: [Histonet] RE: cell block staining issues In-Reply-To: References: <52720D58.8010301@upmc.fr> Message-ID: We have not used B72.3 for quite awhile now but all of our NGYNs are either collected in or processed with Cytolyt and we have had no issues. Sorry, I know this isn't much help. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martha Ward-Pathology Sent: Thursday, October 31, 2013 8:19 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cell block staining issues We have something of a mystery here and I am hoping someone can help. My cytopath docs are saying that our B72.3 (TAG-72) antibody is not working well in cell blocks created from fluids (plural), etc. and that this is a fairly recent development (over the past 5-6 weeks or so). The surgical cases we have stained look great as does our control, which is placed on each slide. We have not changed lot numbers of antibody or made any up recently and the antibody is not expired, or even close. The only thing we can find that is different is that cytology has been rinsing the needles out with a product called Cytolyt, instead of saline and they started this around the first of August. Does anyone know if this product could cause any interference with our staining? The docs just say that the tumor cells are not staining and we are sort of at a loss here. Thanks in advance for your help. Martha Ward, MT (ASCP) QIHC Manager [Wake Forest Baptist Health] Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mward@wakehealth.edu This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From mward <@t> wakehealth.edu Thu Oct 31 08:25:01 2013 From: mward <@t> wakehealth.edu (Martha Ward-Pathology) Date: Thu Oct 31 08:25:06 2013 Subject: [Histonet] RE: cell block staining issues In-Reply-To: References: <52720D58.8010301@upmc.fr> Message-ID: Well actually it does help because we are trying to eliminate possible causes and it is nice to know that someone else uses Cytolyt with no problems. It is just frustrating because we are doing nothing different that I can find. The next step is to see if the samples are coming from the same doctor/clinic and see if they are doing anything different. Martha -----Original Message----- From: Tom McNemar [mailto:TMcNemar@lmhealth.org] Sent: Thursday, October 31, 2013 9:17 AM To: Martha Ward-Pathology; histonet@lists.utsouthwestern.edu Subject: RE: cell block staining issues We have not used B72.3 for quite awhile now but all of our NGYNs are either collected in or processed with Cytolyt and we have had no issues. Sorry, I know this isn't much help. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martha Ward-Pathology Sent: Thursday, October 31, 2013 8:19 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cell block staining issues We have something of a mystery here and I am hoping someone can help. My cytopath docs are saying that our B72.3 (TAG-72) antibody is not working well in cell blocks created from fluids (plural), etc. and that this is a fairly recent development (over the past 5-6 weeks or so). The surgical cases we have stained look great as does our control, which is placed on each slide. We have not changed lot numbers of antibody or made any up recently and the antibody is not expired, or even close. The only thing we can find that is different is that cytology has been rinsing the needles out with a product called Cytolyt, instead of saline and they started this around the first of August. Does anyone know if this product could cause any interference with our staining? The docs just say that the tumor cells are not staining and we are sort of at a loss here. Thanks in advance for your help. Martha Ward, MT (ASCP) QIHC Manager [Wake Forest Baptist Health] Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mward@wakehealth.edu This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From billodonnell <@t> catholichealth.net Thu Oct 31 09:05:28 2013 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Thu Oct 31 09:05:38 2013 Subject: [Histonet] Thermo Slide Mate Message-ID: A note to vendors - I am in need of a roll of ink for the Thermo Slide Mate. I am told that our distributer is out and it will be 4-6 weeks wait. This is an unacceptable wait. Who else is making/marketing this product? I will gladly make a trial purchase. Contact me directly via email bill@deaconbill.com . Please do not post to Histonet - thanks - Bill William (Bill) O'Donnell, HT (ASCP) QIHC Senior Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 SERENITY is not freedom from the storm, but peace amid the storm. This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. From jerrysedgewick <@t> gmail.com Thu Oct 31 10:22:47 2013 From: jerrysedgewick <@t> gmail.com (jerry sedgewick) Date: Thu Oct 31 10:22:59 2013 Subject: [Histonet] 6 biggest problems with color brightfield images In-Reply-To: <1188707850.10382332.1383142577992.JavaMail.root@comcast.net> References: <1188707850.10382332.1383142577992.JavaMail.root@comcast.net> Message-ID: <527275C7.40403@gmail.com> Just in case you're interested, I have written an easy-to-follow guide on 6 problems with color brightfield images, and 6 solutions. See the posting at a blogsite: http://color-integrity.com/?p=15 Enjoy, Jerry Sedgewick From jerrysedgewick <@t> gmail.com Thu Oct 31 10:24:07 2013 From: jerrysedgewick <@t> gmail.com (jerry sedgewick) Date: Thu Oct 31 10:24:18 2013 Subject: [Histonet] 6 biggest problems with color brightfield images In-Reply-To: <1188707850.10382332.1383142577992.JavaMail.root@comcast.net> References: <1188707850.10382332.1383142577992.JavaMail.root@comcast.net> Message-ID: <52727617.9050108@gmail.com> The link in the last email was incorrect. Here is the correct link: http://color-integrity.com/?p=61 From hans <@t> histologistics.com Thu Oct 31 11:56:05 2013 From: hans <@t> histologistics.com (Hans B Snyder) Date: Thu Oct 31 11:56:14 2013 Subject: [Histonet] StainMate Stainer Message-ID: Hello, Does anyone know if the Stainmate stainer can be programed with 50 steps or more? I have looking to buy one but need it for a purpose other than cytology and histology. It needs to be have 50 steps or more for this application. Thank you Hans B Snyder Histologistics 60 Prescott Street Worcester, MA 01605 508-308-7800 hans@histologistics.com From lharris <@t> samhealth.org Thu Oct 31 13:01:59 2013 From: lharris <@t> samhealth.org (Lori Harris) Date: Thu Oct 31 13:02:04 2013 Subject: [Histonet] RE: Ideal Workflow Lab Design for Histo/Cyto lab Processes In-Reply-To: References: Message-ID: <450EDC37E404D142AF67D7314C954C8A3C8474B5F9@SHSMAILVI01.int.samhealth.net> I would also be interested. Thanks. Lori A. Harris, HT (ASCP) 541-768-6078 Histology Section Lead GSRMC Pathology Lab 3600 NW Samaritan Drive Corvallis, OR 97330 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ian R Bernard Sent: Thursday, October 31, 2013 4:48 AM To: Histonet@lists.utsouthwestern.edu Cc: BERNARD, IAN R MSgt USAF USAFA 10 MDSS/SGSH Subject: [Histonet] Ideal Workflow Lab Design for Histo/Cyto lab Processes Histonetters, I'm looking for information or references concerning the ideal workflow design for a Histo/Cyto lab services. Need to consider ergonomics, work space, equipment, safety, etc. I know there are info. Just need source and experts to chime in. Journal of Histotechnology Advance article etc. Please reply to my work email as well. Ian Bernard _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From sae2001 <@t> med.cornell.edu Thu Oct 31 13:11:12 2013 From: sae2001 <@t> med.cornell.edu (Scott A. Ely) Date: Thu Oct 31 13:11:42 2013 Subject: [Histonet] RE: Histonet Digest, Vol 119, Issue 35 In-Reply-To: References: Message-ID: We have tried both and got consistently better results with Leica. In addition to the better results, we have found their support much better. I have been told the same story, Leica>>>Ventana, by colleagues at other institutions. I hope you find this helpful. ------------------------------------------------------------------------------- Scott Ely, MD MPH Associate Director, Hematopathology Fellowship Program Section of Hematopathology Department of Pathology Weill Medical College of Cornell University New York Presbyterian Hospital Room: Starr 715 525 E. 68th Street New York, NY 10065 PH: 212-746-2442 FAX: 212-746-2009 http://www.cornellphysicians.com/scottely/ Legal Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the original intended recipient(s) selected by Dr. Ely and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the recipient specified by Dr. Ely, please contact the sender by reply e-mail and destroy all copies of the original message. ------------------------------------------------------------------------------- ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of histonet-request@lists.utsouthwestern.edu [histonet-request@lists.utsouthwestern.edu] Sent: Thursday, October 31, 2013 1:06 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 119, Issue 35 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: Did you see the listings concerning payday loans????????? (joelle weaver) 2. Re: Kisser's Mounting Media (Glycerol jelly) (Paul Scott) 3. esterase (Stephen KumJew) 4. Kappa and Lambda ISH (Weems, Joyce K.) 5. RE: Ventana vs. Leica IHC stainers (Tony Reilly) 6. Remove OCT from frozen tissue block (Beno?t Delatour) 7. re(2); 33 (escott8@comcast.net) 8. Re: Remove OCT from frozen tissue block (Paula Sicurello) 9. Ideal Workflow Lab Design for Histo/Cyto lab Processes (Ian R Bernard) 10. cell block staining issues (Martha Ward-Pathology) 11. RE: cell block staining issues (Tom McNemar) 12. RE: cell block staining issues (Martha Ward-Pathology) 13. Thermo Slide Mate (O'Donnell, Bill) 14. 6 biggest problems with color brightfield images (jerry sedgewick) 15. 6 biggest problems with color brightfield images (jerry sedgewick) 16. StainMate Stainer (Hans B Snyder) ---------------------------------------------------------------------- Message: 1 Date: Wed, 30 Oct 2013 19:19:57 +0000 From: joelle weaver Subject: RE: [Histonet] Did you see the listings concerning payday loans????????? To: ihcs-wojcieszyn , "Histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" I get that post & link about once per week via the histonet Joelle Weaver MAOM, HTL (ASCP) QIHC > Date: Tue, 29 Oct 2013 20:32:50 -0600 > From: ihcs@smithsys.net > To: Histonet@lists.utsouthwestern.edu > CC: > Subject: [Histonet] Did you see the listings concerning payday loans????????? > > > Why this even get on your system??????????? > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Wed, 30 Oct 2013 13:26:55 -0700 From: "Paul Scott" Subject: [Histonet] Re: Kisser's Mounting Media (Glycerol jelly) To: Message-ID: <002601ced5ae$6041bdb0$20c53910$@com> Content-Type: text/plain; charset="us-ascii" Hi Histonetters, My company makes a temporary coverslip sealant called CytoBond that was designed as a superior replacement for rubber cement in FISH but we are getting increasing interest for IHC applications. The seal withstands high temperatures without requiring humidification and freezing, yet is easily removed in one piece. CytoBond has been on the market a couple of years with an ever increasing customer base. Full product information available here: http://www.scigene.com/documents/M225_CytoBond.pdf and here http://www.scigene.com/details.php?pid=1216 I can send FREE samples to interested parties, you can contact me using the information below. Many thanks, paul Paul Scott SciGene 470 Lakeside Drive, Ste F, Sunnyvale, CA 94085-4720 408-733-7337 x 305 pscott@scigene.com Automating FISH and CMA Workflows www.SciGene.com ------------------------------ Message: 3 Date: Wed, 30 Oct 2013 21:40:57 +0000 From: Stephen KumJew Subject: [Histonet] esterase To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" I was wondering if anyone knew of an esterase stain that doesn't stain the neuromuscular junction too dark. Have tried a modified technique by BJ Davis which uses naphthyl acetate, acetone 0.2M sodium phosphate and azotised pararosaniline but the staining was a dark red/black. Tried reducing times and temperature and reducing the pararosaniline but no change. Thanks Stephen STEPHEN KUM JEW | Senior Technical Officer Discipline of Pathology School of Medical Sciences | Blackburn Building | D06, The University of Sydney | NSW | 2006 T +61 2 9036 9027| F +61 2 9351 3429 E stephen.jew@sydney.edu.au | W http://sydney.edu.au/medicine/pathology/ This email plus any attachments to it are confidential. Any unauthorised use is strictly prohibited. If you receive this email in error, please delete it and any attachments. Please consider the environment before printing this email. ------------------------------ Message: 4 Date: Wed, 30 Oct 2013 21:57:39 +0000 From: "Weems, Joyce K." Subject: [Histonet] Kappa and Lambda ISH To: Histonet Message-ID: Content-Type: text/plain; charset="us-ascii" For those of you doing these successfully on bone marrows - aspirate and biopsy, would you please contact me off the list? We're having issues with the tissues... Thanks for your help! Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). ------------------------------ Message: 5 Date: Thu, 31 Oct 2013 09:16:43 +1000 From: Tony Reilly Subject: [Histonet] RE: Ventana vs. Leica IHC stainers To: Histology , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Hi Mehndi I have worked in labs that have used either the Ventana or the Bond. I have also been involved in evaluating both side by side in my current lab. As far as IHC is involved the Bond staining is in some case stronger but no more sensitive so it depends if you want punchy staining or more delicate staining. When asked our pathologists did not have a preference and were happy to get either instrument. Not sure about the Bond now but when we evaluated in 2010 their CISH was not up and working. We are very happy with the SISH on the Ventana. If you only want to do IHC either will do the job and you should make your judgement on price and after sales customer service. They will both want to charge you for their proprietary reagents based on your workload. If you have a large workload that is fine, but if you have a relatively small workload you may be better off looking at some of the open IHC systems available. Regards Tony Tony Reilly B.App.Sc. , M.Sc. Chief Scientist, Anatomical Pathology Pathology Queensland-PA Laboratory ________________________________________________ Health Services Support Agency | Department of Health Level 1, Building 15,Princess Alexandra Hospital Ipswich Road,WOOLLOONGABBA Qld 4102 Ph: 07 3176 2412 Mob: 0402 139411 Fax: 07 3176 2930 Email: tony.reilly@health.qld.gov.au Web: www.health.qld.gov.au/qhcss/ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Histology Sent: Thursday, 31 October 2013 2:00 AM To: Tony Reilly; histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana vs. Leica IHC stainers Hi all, I'm trying to decide between a Ventana Ultra and a Leica Bond IHC stainer. Has anyone tried both of these? If so, care to share your thoughts on both? Particularly interested in hazardous waste, tissue washing, and DIF procedures. Thanks to all in advance!! Mehndi Helgren Dominion Pathology Laboratories 733 Boush St. Suite 200 Norfolk, VA 23510 757-664-7901 histo@pathlab.us _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. 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Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ********************************************************************************** ------------------------------ Message: 6 Date: Thu, 31 Oct 2013 08:57:12 +0100 From: Beno?t Delatour Subject: [Histonet] Remove OCT from frozen tissue block To: histonet@lists.utsouthwestern.edu Message-ID: <52720D58.8010301@upmc.fr> Content-Type: text/plain; charset=UTF-8; format=flowed Dear histoneters, We got mice brains that were initially sucrose-cryoprotected and then embedded in OCT - flash frozen and stored at -80??C. These tissues were initially prepared for subsequent cryostat sectioning but due to technical considerations we need to cut them using a sliding microtome to get thick (40??m) floating sections. We therefore would like to remove OCT before cutting. As OCT is water soluble it is expected that placing the OCT blocks in buffer would help removing the embedding media but thawing the tissue and then re-freezing it on the microtome stage might not be the best way to proceed (expected formation of ice crystals with known associated histological artefacts)... We would appreciate any alternative solutions. Thanks! Beno??t ------------------------------ Message: 7 Date: Thu, 31 Oct 2013 08:21:45 +0000 (UTC) From: escott8@comcast.net Subject: [Histonet] re(2); 33 To: histonet@lists.utsouthwestern.edu, Joyce.Weems@emoryhealthcare.org, childrenfirst75@aol.com, lilzkin4@icloud.com, escott8@comcast.net Message-ID: <1193952506.13210.1383207705065.JavaMail.root@comcast.net> Content-Type: text/plain; charset=utf-8 referral program http://homegate-ng.com/Scripts/TATI.php Sent: 10/31/2013 1:21:37 AM >From escott8 ------------------------------ Message: 8 Date: Thu, 31 Oct 2013 07:14:45 -0400 From: Paula Sicurello Subject: Re: [Histonet] Remove OCT from frozen tissue block To: benoit.delatour@upmc.fr Cc: HistoNet Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hi Benoit, You can just let the OCT thaw at room temperature then blot away the melted OCT. To speed up the thawing process carefully cut away as much of the OCT as you can with a scalpel or razor blade and then let it thaw. I hope this information is helpful. Paula On Thu, Oct 31, 2013 at 3:57 AM, Beno?t Delatour wrote: > Dear histoneters, > We got mice brains that were initially sucrose-cryoprotected and then > embedded in OCT - flash frozen and stored at -80?C. These tissues were > initially prepared for subsequent cryostat sectioning but due to technical > considerations we need to cut them using a sliding microtome to get thick > (40?m) floating sections. We therefore would like to remove OCT before > cutting. As OCT is water soluble it is expected that placing the OCT blocks > in buffer would help removing the embedding media but thawing the tissue > and then re-freezing it on the microtome stage might not be the best way to > proceed (expected formation of ice crystals with known associated > histological artefacts)... > We would appreciate any alternative solutions. Thanks! > Beno?t > > > ______________________________**_________________ > Histonet mailing list > Histonet@lists.utsouthwestern.**edu > http://lists.utsouthwestern.**edu/mailman/listinfo/histonet > -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 HIPAA Privacy Notification: This message and any accompanying documents are covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, and contain information intended for the specific individual (s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying or the taking of any action based on the contents of this information is strictly prohibited . If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. ------------------------------ Message: 9 Date: Thu, 31 Oct 2013 11:47:30 +0000 From: Ian R Bernard Subject: [Histonet] Ideal Workflow Lab Design for Histo/Cyto lab Processes To: "Histonet@lists.utsouthwestern.edu" Cc: "BERNARD, IAN R MSgt USAF USAFA 10 MDSS/SGSH" Message-ID: Content-Type: text/plain; charset="us-ascii" Histonetters, I'm looking for information or references concerning the ideal workflow design for a Histo/Cyto lab services. Need to consider ergonomics, work space, equipment, safety, etc. I know there are info. Just need source and experts to chime in. Journal of Histotechnology Advance article etc. Please reply to my work email as well. Ian Bernard ------------------------------ Message: 10 Date: Thu, 31 Oct 2013 12:18:36 +0000 From: Martha Ward-Pathology Subject: [Histonet] cell block staining issues To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="utf-8" We have something of a mystery here and I am hoping someone can help. My cytopath docs are saying that our B72.3 (TAG-72) antibody is not working well in cell blocks created from fluids (plural), etc. and that this is a fairly recent development (over the past 5-6 weeks or so). The surgical cases we have stained look great as does our control, which is placed on each slide. We have not changed lot numbers of antibody or made any up recently and the antibody is not expired, or even close. The only thing we can find that is different is that cytology has been rinsing the needles out with a product called Cytolyt, instead of saline and they started this around the first of August. Does anyone know if this product could cause any interference with our staining? The docs just say that the tumor cells are not staining and we are sort of at a loss here. Thanks in advance for your help. Martha Ward, MT (ASCP) QIHC Manager [Wake Forest Baptist Health] Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mward@wakehealth.edu ------------------------------ Message: 11 Date: Thu, 31 Oct 2013 09:16:42 -0400 From: Tom McNemar Subject: [Histonet] RE: cell block staining issues To: 'Martha Ward-Pathology' , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="utf-8" We have not used B72.3 for quite awhile now but all of our NGYNs are either collected in or processed with Cytolyt and we have had no issues. Sorry, I know this isn't much help. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martha Ward-Pathology Sent: Thursday, October 31, 2013 8:19 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cell block staining issues We have something of a mystery here and I am hoping someone can help. My cytopath docs are saying that our B72.3 (TAG-72) antibody is not working well in cell blocks created from fluids (plural), etc. and that this is a fairly recent development (over the past 5-6 weeks or so). The surgical cases we have stained look great as does our control, which is placed on each slide. We have not changed lot numbers of antibody or made any up recently and the antibody is not expired, or even close. The only thing we can find that is different is that cytology has been rinsing the needles out with a product called Cytolyt, instead of saline and they started this around the first of August. Does anyone know if this product could cause any interference with our staining? The docs just say that the tumor cells are not staining and we are sort of at a loss here. Thanks in advance for your help. Martha Ward, MT (ASCP) QIHC Manager [Wake Forest Baptist Health] Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mward@wakehealth.edu This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ------------------------------ Message: 12 Date: Thu, 31 Oct 2013 13:25:01 +0000 From: Martha Ward-Pathology Subject: [Histonet] RE: cell block staining issues To: Tom McNemar , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="utf-8" Well actually it does help because we are trying to eliminate possible causes and it is nice to know that someone else uses Cytolyt with no problems. It is just frustrating because we are doing nothing different that I can find. The next step is to see if the samples are coming from the same doctor/clinic and see if they are doing anything different. Martha -----Original Message----- From: Tom McNemar [mailto:TMcNemar@lmhealth.org] Sent: Thursday, October 31, 2013 9:17 AM To: Martha Ward-Pathology; histonet@lists.utsouthwestern.edu Subject: RE: cell block staining issues We have not used B72.3 for quite awhile now but all of our NGYNs are either collected in or processed with Cytolyt and we have had no issues. Sorry, I know this isn't much help. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martha Ward-Pathology Sent: Thursday, October 31, 2013 8:19 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cell block staining issues We have something of a mystery here and I am hoping someone can help. My cytopath docs are saying that our B72.3 (TAG-72) antibody is not working well in cell blocks created from fluids (plural), etc. and that this is a fairly recent development (over the past 5-6 weeks or so). The surgical cases we have stained look great as does our control, which is placed on each slide. We have not changed lot numbers of antibody or made any up recently and the antibody is not expired, or even close. The only thing we can find that is different is that cytology has been rinsing the needles out with a product called Cytolyt, instead of saline and they started this around the first of August. Does anyone know if this product could cause any interference with our staining? The docs just say that the tumor cells are not staining and we are sort of at a loss here. Thanks in advance for your help. Martha Ward, MT (ASCP) QIHC Manager [Wake Forest Baptist Health] Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mward@wakehealth.edu This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ------------------------------ Message: 13 Date: Thu, 31 Oct 2013 14:05:28 +0000 From: "O'Donnell, Bill" Subject: [Histonet] Thermo Slide Mate To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" A note to vendors - I am in need of a roll of ink for the Thermo Slide Mate. I am told that our distributer is out and it will be 4-6 weeks wait. This is an unacceptable wait. Who else is making/marketing this product? I will gladly make a trial purchase. Contact me directly via email bill@deaconbill.com . Please do not post to Histonet - thanks - Bill William (Bill) O'Donnell, HT (ASCP) QIHC Senior Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 SERENITY is not freedom from the storm, but peace amid the storm. This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. ------------------------------ Message: 14 Date: Thu, 31 Oct 2013 10:22:47 -0500 From: jerry sedgewick Subject: [Histonet] 6 biggest problems with color brightfield images To: histonet@lists.utsouthwestern.edu Message-ID: <527275C7.40403@gmail.com> Content-Type: text/plain; charset=UTF-8; format=flowed Just in case you're interested, I have written an easy-to-follow guide on 6 problems with color brightfield images, and 6 solutions. See the posting at a blogsite: http://color-integrity.com/?p=15 Enjoy, Jerry Sedgewick ------------------------------ Message: 15 Date: Thu, 31 Oct 2013 10:24:07 -0500 From: jerry sedgewick Subject: [Histonet] 6 biggest problems with color brightfield images To: histonet@lists.utsouthwestern.edu Message-ID: <52727617.9050108@gmail.com> Content-Type: text/plain; charset=UTF-8; format=flowed The link in the last email was incorrect. Here is the correct link: http://color-integrity.com/?p=61 ------------------------------ Message: 16 Date: Thu, 31 Oct 2013 12:56:05 -0400 From: Hans B Snyder Subject: [Histonet] StainMate Stainer To: Histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hello, Does anyone know if the Stainmate stainer can be programed with 50 steps or more? I have looking to buy one but need it for a purpose other than cytology and histology. It needs to be have 50 steps or more for this application. Thank you Hans B Snyder Histologistics 60 Prescott Street Worcester, MA 01605 508-308-7800 hans@histologistics.com ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 119, Issue 35 ***************************************** From mroark <@t> sfmc.net Thu Oct 31 15:21:47 2013 From: mroark <@t> sfmc.net (Matthew D. Roark) Date: Thu Oct 31 15:21:57 2013 Subject: [Histonet] Specimen Tracking Systems Message-ID: <3e87cff0fdcd4c46bd90911b828a42f5@BLUPR04MB199.namprd04.prod.outlook.com> What systems are people using for specimen tracking? I'm looking for the whole package of cassette printers, work station slide printers, tracking software, etc... Any help and recommendations would be appreciated! Matthew Roark- HT/HTL(ASCP)CM Histology Specialist Saint Francis Medical Center 211 Saint Francis Drive Cape Girardeau, MO 63703 573-331-3982 mroark@sfmc.net http://www.sfmc.net From Richard.Cartun <@t> hhchealth.org Thu Oct 31 15:44:56 2013 From: Richard.Cartun <@t> hhchealth.org (Cartun, Richard) Date: Thu Oct 31 15:45:06 2013 Subject: [Histonet] Pancreatic tryptase Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E0188CB80@HHCEXCHMB05.hhcsystem.org> I may have asked this question before, but, if I did, I did not receive any replies. Is anyone doing IHC for pancreatic tryptase and, if so, where do you get your antibody? Thank you! Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax richard.cartun@hhchealth.org This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From wdesalvo.cac <@t> outlook.com Thu Oct 31 17:00:46 2013 From: wdesalvo.cac <@t> outlook.com (WILLIAM DESALVO) Date: Thu Oct 31 17:00:58 2013 Subject: [Histonet] Specimen Tracking Systems In-Reply-To: <3e87cff0fdcd4c46bd90911b828a42f5@BLUPR04MB199.namprd04.prod.outlook.com> References: <3e87cff0fdcd4c46bd90911b828a42f5@BLUPR04MB199.namprd04.prod.outlook.com> Message-ID: My lab chose the Ventana Vantage System w/ Thermo PrintMate cassette printers. We are a multi-location system and Vantage works well for us. We wanted a robust tracking and flexible quality assurance system and Ventana provided what we needed and wanted. I suggest you look at the big four to start your evaluation: Ventana - Vantage Leica- Cerebro Thermo- OmniTrax General Data - HTS The larger companies have more interfaces built for the different LIS used in Histology. There are many smaller companies that offer tracking systems, but make sure they can connect w/ your LIS. After product selection, support is critical. Sunquest and Cerner Millennium now offer tracking and quality assurance modules. And I imagine there are other tracking systems built into more LIS. You will also want to evaluate multiple printers for your cassettes. Always keep in mind the amount of information you will be adding to the cassette a this will reduce the number of cassettes/ minute. Start w/ the tracking system vendor and see what the have validated. Consider the upkeep and maintenance of the printer. We choose heat transfer, but there are Laser and Ink-jet that work really well. We chose to point of generation and wanted a very small footprint to place the printers in the grossing stations. We also choose not to use a slide printing system and decided the bar coded labels at microtomy was the best solution for us. We wanted less maintenance and steady throughput. Easier to load a roll of labels than keeping a printer up and running. Last, but certainly not least, support. You will need a ton of support to work through this project: interfaces, IT upgrades, software adjustments, WORKFLOW changes and training. Plan and re-plan before you make your selection. And negotiate hard, the pricing is all over the place for the systems. William DeSalvo, BS HTL(ASCP) Production Manager-Anatomic Pathology Chair, NSH Quality Management Committee Board Member, Digital Pathology Association Owner/Consultant, Collaborative Advantage Consulting > From: mroark@sfmc.net > To: histonet@lists.utsouthwestern.edu > Date: Thu, 31 Oct 2013 20:21:47 +0000 > Subject: [Histonet] Specimen Tracking Systems > > What systems are people using for specimen tracking? I'm looking for the whole package of cassette printers, work station slide printers, tracking software, etc... > > Any help and recommendations would be appreciated! > > > > Matthew Roark- HT/HTL(ASCP)CM > Histology Specialist > Saint Francis Medical Center > 211 Saint Francis Drive > Cape Girardeau, MO 63703 > 573-331-3982 > mroark@sfmc.net > http://www.sfmc.net > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Thu Oct 31 17:44:49 2013 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Thu Oct 31 18:01:59 2013 Subject: [Histonet] Specimen Tracking Systems In-Reply-To: References: <3e87cff0fdcd4c46bd90911b828a42f5@BLUPR04MB199.namprd04.prod.outlook.com> Message-ID: <761E2B5697F795489C8710BCC72141FF0AE4FF@ex07.net.ucsf.edu> I agree with Bill on this. We have Cerner Copath Plus and looked at Cerebro, Vantage and Omnitrax. Only Cerebro and Vantage were capable of working with Copath. But the problem is that Copath requires you purchase their tracking system to use as an HL-7 interface to any third party system. AND there is not two-way communication between the systems. You MUST enter orders (cases, blocks, stains) in copath and it ports orders to the other system. The only feedback is status updates to copath - you cannot add items in the other system and expect them to show up in copath. That requirement effectively doubled the price of the system. So we are using only the Copath AB&T system, which we are starting to implement now. They will finally have touch screen capability in the spring so we were satisfied with that. Touch screens were a primary spec we demanded. The key to tracking is how the tracking system works with your current LIS. Can it communicate in a true bi-directional fashion? Is it just an add on and all the tracking info is in the tracking component, not the LIS? There are tradeoffs and you need to figure out which you want to live with. We spent two years investigating all these systems, doing site visits, and going through a 6-month total LEAN evaluation. The one thing every institution told us was: LEAN your system BEFORE adding barcoding. Barcoding will NOT fix any inefficiencies you have. And you are pretty much stuck with what ever system you barcode - warts and all. So do all the hard work up front. We thought we were prepared but are daily finding all kinds of little details that have to be worked out. Detail out every single little thing about all your workflows before starting anything. Streamline everything. Cassette printing is the heart of barcoding. The barcoded (actually usually 2D matrix codes) on the cassette drive grossing, tracking through processing, embedding and cutting. If that does not work reliably your system will not work. We went with Leica cassette printers. They are huge, but fast (5 sec per cass). We were going to use thermo, but they had their printhead problem and we dropped the order. They are 20+ seconds per cassttes, tho have a very compact footprint. You will find there are tradeoffs to everything. Slide labeling is the other half of the equation. They must be reliable and survive all histology procedures. We will use thermal transfer label printers rather than direct slide (too expensive- we will have a printer at each microtome- and cumbersome to change out slide types). Slide labeling is a HUGE deal - you have to be sure it works for everything and can be used in your immuno stainers and coverslipping machines. We are using either Brady StainerBondz labels or LabTags Xylituff labels. These were the only ones that had the size we wanted (23 x 19 mm) and survived all processes. General data StainerShield are also good, but only come in 22 x 22 size. Plus are the most expensive. You will find it much easier if you use labels and ribbons from one manufacturer, designed to use with specific printers. That simplifies a lot of the effort. We have tried to mix and match but it is very difficult because all manufacturers do not make matching labels and ribbons for all printers. Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO Sent: Thursday, October 31, 2013 3:01 PM To: Matthew D. Roark; histonet Subject: RE: [Histonet] Specimen Tracking Systems My lab chose the Ventana Vantage System w/ Thermo PrintMate cassette printers. We are a multi-location system and Vantage works well for us. We wanted a robust tracking and flexible quality assurance system and Ventana provided what we needed and wanted. I suggest you look at the big four to start your evaluation: Ventana - Vantage Leica- Cerebro Thermo- OmniTrax General Data - HTS The larger companies have more interfaces built for the different LIS used in Histology. There are many smaller companies that offer tracking systems, but make sure they can connect w/ your LIS. After product selection, support is critical. Sunquest and Cerner Millennium now offer tracking and quality assurance modules. And I imagine there are other tracking systems built into more LIS. You will also want to evaluate multiple printers for your cassettes. Always keep in mind the amount of information you will be adding to the cassette a this will reduce the number of cassettes/ minute. Start w/ the tracking system vendor and see what the have validated. Consider the upkeep and maintenance of the printer. We choose heat transfer, but there are Laser and Ink-jet that work really well. We chose to point of generation and wanted a very small footprint to place the printers in the grossing stations. We also choose not to use a slide printing system and decided the bar coded labels at microtomy was the best solution for us. We wanted less maintenance and steady throughput. Easier to load a roll of labels than keeping a printer up and running. Last, but certainly not least, support. You will need a ton of support to work through this project: interfaces, IT upgrades, software adjustments, WORKFLOW changes and training. Plan and re-plan before you make your selection. And negotiate hard, the pricing is all over the place for the systems. William DeSalvo, BS HTL(ASCP) Production Manager-Anatomic Pathology Chair, NSH Quality Management Committee Board Member, Digital Pathology Association Owner/Consultant, Collaborative Advantage Consulting > From: mroark@sfmc.net > To: histonet@lists.utsouthwestern.edu > Date: Thu, 31 Oct 2013 20:21:47 +0000 > Subject: [Histonet] Specimen Tracking Systems > > What systems are people using for specimen tracking? I'm looking for the whole package of cassette printers, work station slide printers, tracking software, etc... > > Any help and recommendations would be appreciated! > > > > Matthew Roark- HT/HTL(ASCP)CM > Histology Specialist > Saint Francis Medical Center > 211 Saint Francis Drive > Cape Girardeau, MO 63703 > 573-331-3982 > mroark@sfmc.net > http://www.sfmc.net > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet