[Histonet] RE: F480 antibody staining

[Elizabeth Chlipala]

Cindy

We use that antibody with dako's rabbit anti-rat (mouse absorbed) and then envision rabbit after that, we use proteinase K for digestion.  If you don't want to use the polymer reagent you can use strep avidin instead.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 881-0763 cell
(303) 682-9060 fax
liz <@t> premierlab.com

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Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Cynthia Hutchinson [tilycyn <@t> auburn.edu]
Sent: Friday, November 22, 2013 3:29 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] F480 antibody staining

Can anyone share a clean staining protocol utilizing a rat monoclonal (I have rat anti mouse cloneCI:A3-1 from AbD) on mouse tissues?
Thanks,
Cindy
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