[Histonet] PDL-1/PD-1

[Milne, Katy]

Hi Pat, 

We've been working with PD-1 and PD-L1.

For PD-1, we use the MRQ-22 clone from Cell Marque, it has worked really well for us.
We use it at 1/250 for 30min with Biocare reagents (Mach2 Mouse polymers), I've been doing mostly multicolour IHC with it.

For PD-L1 we use a mouse MAb from MBL cat # D230-3 clone 27A2.  It was the same Ab used in this paper - PMID 17360651
I've tried a few different concentrations with this one so it's best to see what it does in your hands.  In our hands, most of our samples come up positive (ovarian cancer) but it's not as striking as it would be for membrane or nuclear markers.

If you have any other questions, please let me know.

Katy


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Message: 3
Date: Wed, 20 Nov 2013 12:35:40 -0700
From: "Bell, Pat" <Pat.Bell <@t> ucdenver.edu>
Subject: [Histonet] PDL-1/PD-1
To: "histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>
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	<64DB27005E2FD3439E88502D7A5C912101024B94AE27 <@t> CORTEZ.ucdenver.pvt>
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Hello to everyone!

Has anyone used PDL-1/PD-1 on FFPE tissue?  Could I please get a copy of your protocol and where you purchased the antibody?

Thank you so much.
Pat

Pat Bell HT(ASCP)
Sr. PRA
University of Colorado, Denver
Medical Oncology; MS 8117
12801 E 17th Ave
Aurora, Colorado 80045
303-724-6077
pat.bell <@t> ucdenver.edu<mailto:pat.bell <@t> ucdenver.edu>