From joelleweaver <@t> hotmail.com Fri Nov 1 07:14:54 2013 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Fri Nov 1 07:14:58 2013 Subject: [Histonet] Specimen Tracking Systems In-Reply-To: <761E2B5697F795489C8710BCC72141FF0AE4FF@ex07.net.ucsf.edu> References: <3e87cff0fdcd4c46bd90911b828a42f5@BLUPR04MB199.namprd04.prod.outlook.com>, , <761E2B5697F795489C8710BCC72141FF0AE4FF@ex07.net.ucsf.edu> Message-ID: Thanks Tim, you have excellent points and advice. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: Timothy.Morken@ucsfmedctr.org > To: wdesalvo.cac@outlook.com; mroark@sfmc.net; histonet@lists.utsouthwestern.edu > Date: Thu, 31 Oct 2013 22:44:49 +0000 > Subject: RE: [Histonet] Specimen Tracking Systems > CC: > > I agree with Bill on this. We have Cerner Copath Plus and looked at Cerebro, Vantage and Omnitrax. Only Cerebro and Vantage were capable of working with Copath. But the problem is that Copath requires you purchase their tracking system to use as an HL-7 interface to any third party system. AND there is not two-way communication between the systems. You MUST enter orders (cases, blocks, stains) in copath and it ports orders to the other system. The only feedback is status updates to copath - you cannot add items in the other system and expect them to show up in copath. That requirement effectively doubled the price of the system. So we are using only the > Copath AB&T system, which we are starting to implement now. They will finally have touch screen capability in the spring so we were satisfied with that. Touch screens were a primary spec we demanded. > > The key to tracking is how the tracking system works with your current LIS. Can it communicate in a true bi-directional fashion? Is it just an add on and all the tracking info is in the tracking component, not the LIS? There are tradeoffs and you need to figure out which you want to live with. > > We spent two years investigating all these systems, doing site visits, and going through a 6-month total LEAN evaluation. The one thing every institution told us was: LEAN your system BEFORE adding barcoding. Barcoding will NOT fix any inefficiencies you have. And you are pretty much stuck with what ever system you barcode - warts and all. So do all the hard work up front. > > We thought we were prepared but are daily finding all kinds of little details that have to be worked out. Detail out every single little thing about all your workflows before starting anything. Streamline everything. > > Cassette printing is the heart of barcoding. The barcoded (actually usually 2D matrix codes) on the cassette drive grossing, tracking through processing, embedding and cutting. If that does not work reliably your system will not work. > > We went with Leica cassette printers. They are huge, but fast (5 sec per cass). We were going to use thermo, but they had their printhead problem and we dropped the order. They are 20+ seconds per cassttes, tho have a very compact footprint. You will find there are tradeoffs to everything. > > Slide labeling is the other half of the equation. They must be reliable and survive all histology procedures. > > We will use thermal transfer label printers rather than direct slide (too expensive- we will have a printer at each microtome- and cumbersome to change out slide types). Slide labeling is a HUGE deal - you have to be sure it works for everything and can be used in your immuno stainers and coverslipping machines. We are using either Brady StainerBondz labels or LabTags Xylituff labels. These were the only ones that had the size we wanted (23 x 19 mm) and survived all processes. General data StainerShield are also good, but only come in 22 x 22 size. Plus are the most expensive. > > You will find it much easier if you use labels and ribbons from one manufacturer, designed to use with specific printers. That simplifies a lot of the effort. We have tried to mix and match but it is very difficult because all manufacturers do not make matching labels and ribbons for all printers. > > > > Tim Morken > Supervisor, Electron Microscopy and Neuromuscular Special Studies > UC San Francisco Medical Center > San Francisco, CA > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO > Sent: Thursday, October 31, 2013 3:01 PM > To: Matthew D. Roark; histonet > Subject: RE: [Histonet] Specimen Tracking Systems > > My lab chose the Ventana Vantage System w/ Thermo PrintMate cassette printers. We are a multi-location system and Vantage works well for us. We wanted a robust tracking and flexible quality assurance system and Ventana provided what we needed and wanted. > > I suggest you look at the big four to start your evaluation: > Ventana - Vantage > Leica- Cerebro > Thermo- OmniTrax > General Data - HTS > > The larger companies have more interfaces built for the different LIS used in Histology. There are many smaller companies that offer tracking systems, but make sure they can connect w/ your LIS. After product selection, support is critical. > > Sunquest and Cerner Millennium now offer tracking and quality assurance modules. And I imagine there are other tracking systems built into more LIS. > > You will also want to evaluate multiple printers for your cassettes. Always keep in mind the amount of information you will be adding to the cassette a this will reduce the number of cassettes/ minute. Start w/ the tracking system vendor and see what the have validated. Consider the upkeep and maintenance of the printer. We choose heat transfer, but there are Laser and Ink-jet that work really well. We chose to point of generation and wanted a very small footprint to place the printers in the grossing stations. > > We also choose not to use a slide printing system and decided the bar coded labels at microtomy was the best solution for us. We wanted less maintenance and steady throughput. Easier to load a roll of labels than keeping a printer up and running. > > Last, but certainly not least, support. You will need a ton of support to work through this project: interfaces, IT upgrades, software adjustments, WORKFLOW changes and training. Plan and re-plan before you make your selection. And negotiate hard, the pricing is all over the place for the systems. > > > William DeSalvo, BS HTL(ASCP) > Production Manager-Anatomic Pathology > Chair, NSH Quality Management Committee > Board Member, Digital Pathology Association Owner/Consultant, Collaborative Advantage Consulting > > > > From: mroark@sfmc.net > > To: histonet@lists.utsouthwestern.edu > > Date: Thu, 31 Oct 2013 20:21:47 +0000 > > Subject: [Histonet] Specimen Tracking Systems > > > > What systems are people using for specimen tracking? I'm looking for the whole package of cassette printers, work station slide printers, tracking software, etc... > > > > Any help and recommendations would be appreciated! > > > > > > > > Matthew Roark- HT/HTL(ASCP)CM > > Histology Specialist > > Saint Francis Medical Center > > 211 Saint Francis Drive > > Cape Girardeau, MO 63703 > > 573-331-3982 > > mroark@sfmc.net > > http://www.sfmc.net > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From KSimeone <@t> leavittmgt.com Fri Nov 1 07:55:34 2013 From: KSimeone <@t> leavittmgt.com (Delray Beach Pathology Kari Simeone) Date: Fri Nov 1 07:55:58 2013 Subject: [Histonet] Part time Histo Tech position DELRAY BEACH, FLORIDA (in case you missed it) Message-ID: Hi Histonetters! We are still interviewing for a part time tech here in our very busy Delray Derm Lab. This is a permanent part time position with full time growth potential. PLEASE NO HEAD HUNTERS/PLACEMENT SERVICES!!! Email your resume to ksimeone@leavittmgt.com if interested. *part time position Mon-Wed 5p-10p (starting time semi-flexible) also vacation coverage shifts available *MUST be licensed as a FL histotechnologist or technician *MUST have at least 2 years experience (dermatology preferred) *very proficient in embedding and microtomy *experience in grossing and immunohistochemistry (BOND) a plus *must be self motivated and a team player *knowledge in operating Ventana and Leica equipment desired (not necessary) Kari M Simeone Histology/Immunohistochemistry Specialist Supervisor Alternate Laboratory Supervisor ADCS Clinics, LLC Advanced Dermatology and Cosmetic Surgery Ameriderm ksimeone@leavittmgt.com www.advancedderm.com The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message. From Donna.Willis <@t> baylorhealth.edu Fri Nov 1 08:46:51 2013 From: Donna.Willis <@t> baylorhealth.edu (Willis, Donna G.) Date: Fri Nov 1 08:46:57 2013 Subject: [Histonet] Specimen Tracking Systems In-Reply-To: <761E2B5697F795489C8710BCC72141FF0AE4FF@ex07.net.ucsf.edu> References: <3e87cff0fdcd4c46bd90911b828a42f5@BLUPR04MB199.namprd04.prod.outlook.com> <761E2B5697F795489C8710BCC72141FF0AE4FF@ex07.net.ucsf.edu> Message-ID: <2572B4D63B62E64A8078D8BBE34D407801A38FF5@BHDASVEXML2.bhcs.pvt> Tim, What version of Cerner CoPath Plus are you using? We are looking at changing our current LIS. We have Vantage. Thanks, Donna Willis, HT/HTL (ASCP) Anatomic Pathology Manager Baylor University Medical Center-Dallas ph. 214-820-2465 office ph. 214-725-6184 mobile donna.willis@baylorhealth.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Thursday, October 31, 2013 5:45 PM To: 'WILLIAM DESALVO'; Matthew D. Roark; histonet Subject: RE: [Histonet] Specimen Tracking Systems I agree with Bill on this. We have Cerner Copath Plus and looked at Cerebro, Vantage and Omnitrax. Only Cerebro and Vantage were capable of working with Copath. But the problem is that Copath requires you purchase their tracking system to use as an HL-7 interface to any third party system. AND there is not two-way communication between the systems. You MUST enter orders (cases, blocks, stains) in copath and it ports orders to the other system. The only feedback is status updates to copath - you cannot add items in the other system and expect them to show up in copath. That requirement effectively doubled the price of the system. So we are using only the Copath AB&T system, which we are starting to implement now. They will finally have touch screen capability in the spring so we were satisfied with that. Touch screens were a primary spec we demanded. The key to tracking is how the tracking system works with your current LIS. Can it communicate in a true bi-directional fashion? Is it just an add on and all the tracking info is in the tracking component, not the LIS? There are tradeoffs and you need to figure out which you want to live with. We spent two years investigating all these systems, doing site visits, and going through a 6-month total LEAN evaluation. The one thing every institution told us was: LEAN your system BEFORE adding barcoding. Barcoding will NOT fix any inefficiencies you have. And you are pretty much stuck with what ever system you barcode - warts and all. So do all the hard work up front. We thought we were prepared but are daily finding all kinds of little details that have to be worked out. Detail out every single little thing about all your workflows before starting anything. Streamline everything. Cassette printing is the heart of barcoding. The barcoded (actually usually 2D matrix codes) on the cassette drive grossing, tracking through processing, embedding and cutting. If that does not work reliably your system will not work. We went with Leica cassette printers. They are huge, but fast (5 sec per cass). We were going to use thermo, but they had their printhead problem and we dropped the order. They are 20+ seconds per cassttes, tho have a very compact footprint. You will find there are tradeoffs to everything. Slide labeling is the other half of the equation. They must be reliable and survive all histology procedures. We will use thermal transfer label printers rather than direct slide (too expensive- we will have a printer at each microtome- and cumbersome to change out slide types). Slide labeling is a HUGE deal - you have to be sure it works for everything and can be used in your immuno stainers and coverslipping machines. We are using either Brady StainerBondz labels or LabTags Xylituff labels. These were the only ones that had the size we wanted (23 x 19 mm) and survived all processes. General data StainerShield are also good, but only come in 22 x 22 size. Plus are the most expensive. You will find it much easier if you use labels and ribbons from one manufacturer, designed to use with specific printers. That simplifies a lot of the effort. We have tried to mix and match but it is very difficult because all manufacturers do not make matching labels and ribbons for all printers. Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO Sent: Thursday, October 31, 2013 3:01 PM To: Matthew D. Roark; histonet Subject: RE: [Histonet] Specimen Tracking Systems My lab chose the Ventana Vantage System w/ Thermo PrintMate cassette printers. We are a multi-location system and Vantage works well for us. We wanted a robust tracking and flexible quality assurance system and Ventana provided what we needed and wanted. I suggest you look at the big four to start your evaluation: Ventana - Vantage Leica- Cerebro Thermo- OmniTrax General Data - HTS The larger companies have more interfaces built for the different LIS used in Histology. There are many smaller companies that offer tracking systems, but make sure they can connect w/ your LIS. After product selection, support is critical. Sunquest and Cerner Millennium now offer tracking and quality assurance modules. And I imagine there are other tracking systems built into more LIS. You will also want to evaluate multiple printers for your cassettes. Always keep in mind the amount of information you will be adding to the cassette a this will reduce the number of cassettes/ minute. Start w/ the tracking system vendor and see what the have validated. Consider the upkeep and maintenance of the printer. We choose heat transfer, but there are Laser and Ink-jet that work really well. We chose to point of generation and wanted a very small footprint to place the printers in the grossing stations. We also choose not to use a slide printing system and decided the bar coded labels at microtomy was the best solution for us. We wanted less maintenance and steady throughput. Easier to load a roll of labels than keeping a printer up and running. Last, but certainly not least, support. You will need a ton of support to work through this project: interfaces, IT upgrades, software adjustments, WORKFLOW changes and training. Plan and re-plan before you make your selection. And negotiate hard, the pricing is all over the place for the systems. William DeSalvo, BS HTL(ASCP) Production Manager-Anatomic Pathology Chair, NSH Quality Management Committee Board Member, Digital Pathology Association Owner/Consultant, Collaborative Advantage Consulting > From: mroark@sfmc.net > To: histonet@lists.utsouthwestern.edu > Date: Thu, 31 Oct 2013 20:21:47 +0000 > Subject: [Histonet] Specimen Tracking Systems > > What systems are people using for specimen tracking? I'm looking for the whole package of cassette printers, work station slide printers, tracking software, etc... > > Any help and recommendations would be appreciated! > > > > Matthew Roark- HT/HTL(ASCP)CM > Histology Specialist > Saint Francis Medical Center > 211 Saint Francis Drive > Cape Girardeau, MO 63703 > 573-331-3982 > mroark@sfmc.net > https://urldefense.proofpoint.com/v1/url?u=http://www.sfmc.net/&k=eEbcMSXJ22m%2Bwpc%2B3zaDpA%3D%3D%0A&r=tcwXtHSgC9tIe4wFOXToKDTqAni3dMiqUwIdlw6Tfz0%3D%0A&m=mqM4469AscayER1x0EafWMCsIx%2FaNdwu8iUmIE1dnL0%3D%0A&s=304d54960ab388213e6775e21d200758d6b6a5cbbb532fde95d35d582b73d259 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > https://urldefense.proofpoint.com/v1/url?u=http://lists.utsouthwestern.edu/mailman/listinfo/histonet&k=eEbcMSXJ22m%2Bwpc%2B3zaDpA%3D%3D%0A&r=tcwXtHSgC9tIe4wFOXToKDTqAni3dMiqUwIdlw6Tfz0%3D%0A&m=mqM4469AscayER1x0EafWMCsIx%2FaNdwu8iUmIE1dnL0%3D%0A&s=66fc9dc20690db1cc60e961ea00d81571975a436bea1399dd1e83c245965f6fe _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v1/url?u=http://lists.utsouthwestern.edu/mailman/listinfo/histonet&k=eEbcMSXJ22m%2Bwpc%2B3zaDpA%3D%3D%0A&r=tcwXtHSgC9tIe4wFOXToKDTqAni3dMiqUwIdlw6Tfz0%3D%0A&m=mqM4469AscayER1x0EafWMCsIx%2FaNdwu8iUmIE1dnL0%3D%0A&s=66fc9dc20690db1cc60e961ea00d81571975a436bea1399dd1e83c245965f6fe _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v1/url?u=http://lists.utsouthwestern.edu/mailman/listinfo/histonet&k=eEbcMSXJ22m%2Bwpc%2B3zaDpA%3D%3D%0A&r=tcwXtHSgC9tIe4wFOXToKDTqAni3dMiqUwIdlw6Tfz0%3D%0A&m=mqM4469AscayER1x0EafWMCsIx%2FaNdwu8iUmIE1dnL0%3D%0A&s=66fc9dc20690db1cc60e961ea00d81571975a436bea1399dd1e83c245965f6fe ********************************************************************** This e-mail may contain confidential and/or privileged information. 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From JEllin <@t> yumaregional.org Fri Nov 1 08:54:16 2013 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Fri Nov 1 08:54:28 2013 Subject: [Histonet] Specimen Tracking Systems In-Reply-To: <761E2B5697F795489C8710BCC72141FF0AE4FF@ex07.net.ucsf.edu> References: <3e87cff0fdcd4c46bd90911b828a42f5@BLUPR04MB199.namprd04.prod.outlook.com> <761E2B5697F795489C8710BCC72141FF0AE4FF@ex07.net.ucsf.edu> Message-ID: I would like to chime in on this Bill and Tim both gave great examples of what it takes to move in this direction. But I would also like to understand why there are so many challenges in getting the data back into the APLIS with updates and orders. I would tell people the biggest concern that I have the data being produced by a system should be accessible across all gradients of the workflow and not just the unique tracking system. Not only is this tracking system positioned to help out histology with what is being explained below, but it is the foundation work in taking steps to get to the next level when talking Digital Pathology, molecular, Personalized medicine, Pathologist cockpit, and algorithm analysis. With the looming cuts coming down the pike, we have to make sure the vendors understand we cannot have barriers when it comes to interface and interoperability with our system. At the same token we cannot be charges an arm, leg and torso for interfacing what is rightfully ours and that is the patients data we produce. Bill and Tim excellent job in giving a crash course in Tracking system 101,, I do believe we should see more of this explained at professional societies meeting and also what the new environments we are going to be working in because of this technology. Just my two cents Jesus Ellin -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Thursday, October 31, 2013 3:45 PM To: 'WILLIAM DESALVO'; Matthew D. Roark; histonet Subject: RE: [Histonet] Specimen Tracking Systems I agree with Bill on this. We have Cerner Copath Plus and looked at Cerebro, Vantage and Omnitrax. Only Cerebro and Vantage were capable of working with Copath. But the problem is that Copath requires you purchase their tracking system to use as an HL-7 interface to any third party system. AND there is not two-way communication between the systems. You MUST enter orders (cases, blocks, stains) in copath and it ports orders to the other system. The only feedback is status updates to copath - you cannot add items in the other system and expect them to show up in copath. That requirement effectively doubled the price of the system. So we are using only the Copath AB&T system, which we are starting to implement now. They will finally have touch screen capability in the spring so we were satisfied with that. Touch screens were a primary spec we demanded. The key to tracking is how the tracking system works with your current LIS. Can it communicate in a true bi-directional fashion? Is it just an add on and all the tracking info is in the tracking component, not the LIS? There are tradeoffs and you need to figure out which you want to live with. We spent two years investigating all these systems, doing site visits, and going through a 6-month total LEAN evaluation. The one thing every institution told us was: LEAN your system BEFORE adding barcoding. Barcoding will NOT fix any inefficiencies you have. And you are pretty much stuck with what ever system you barcode - warts and all. So do all the hard work up front. We thought we were prepared but are daily finding all kinds of little details that have to be worked out. Detail out every single little thing about all your workflows before starting anything. Streamline everything. Cassette printing is the heart of barcoding. The barcoded (actually usually 2D matrix codes) on the cassette drive grossing, tracking through processing, embedding and cutting. If that does not work reliably your system will not work. We went with Leica cassette printers. They are huge, but fast (5 sec per cass). We were going to use thermo, but they had their printhead problem and we dropped the order. They are 20+ seconds per cassttes, tho have a very compact footprint. You will find there are tradeoffs to everything. Slide labeling is the other half of the equation. They must be reliable and survive all histology procedures. We will use thermal transfer label printers rather than direct slide (too expensive- we will have a printer at each microtome- and cumbersome to change out slide types). Slide labeling is a HUGE deal - you have to be sure it works for everything and can be used in your immuno stainers and coverslipping machines. We are using either Brady StainerBondz labels or LabTags Xylituff labels. These were the only ones that had the size we wanted (23 x 19 mm) and survived all processes. General data StainerShield are also good, but only come in 22 x 22 size. Plus are the most expensive. You will find it much easier if you use labels and ribbons from one manufacturer, designed to use with specific printers. That simplifies a lot of the effort. We have tried to mix and match but it is very difficult because all manufacturers do not make matching labels and ribbons for all printers. Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO Sent: Thursday, October 31, 2013 3:01 PM To: Matthew D. Roark; histonet Subject: RE: [Histonet] Specimen Tracking Systems My lab chose the Ventana Vantage System w/ Thermo PrintMate cassette printers. We are a multi-location system and Vantage works well for us. We wanted a robust tracking and flexible quality assurance system and Ventana provided what we needed and wanted. I suggest you look at the big four to start your evaluation: Ventana - Vantage Leica- Cerebro Thermo- OmniTrax General Data - HTS The larger companies have more interfaces built for the different LIS used in Histology. There are many smaller companies that offer tracking systems, but make sure they can connect w/ your LIS. After product selection, support is critical. Sunquest and Cerner Millennium now offer tracking and quality assurance modules. And I imagine there are other tracking systems built into more LIS. You will also want to evaluate multiple printers for your cassettes. Always keep in mind the amount of information you will be adding to the cassette a this will reduce the number of cassettes/ minute. Start w/ the tracking system vendor and see what the have validated. Consider the upkeep and maintenance of the printer. We choose heat transfer, but there are Laser and Ink-jet that work really well. We chose to point of generation and wanted a very small footprint to place the printers in the grossing stations. We also choose not to use a slide printing system and decided the bar coded labels at microtomy was the best solution for us. We wanted less maintenance and steady throughput. Easier to load a roll of labels than keeping a printer up and running. Last, but certainly not least, support. You will need a ton of support to work through this project: interfaces, IT upgrades, software adjustments, WORKFLOW changes and training. Plan and re-plan before you make your selection. And negotiate hard, the pricing is all over the place for the systems. William DeSalvo, BS HTL(ASCP) Production Manager-Anatomic Pathology Chair, NSH Quality Management Committee Board Member, Digital Pathology Association Owner/Consultant, Collaborative Advantage Consulting > From: mroark@sfmc.net > To: histonet@lists.utsouthwestern.edu > Date: Thu, 31 Oct 2013 20:21:47 +0000 > Subject: [Histonet] Specimen Tracking Systems > > What systems are people using for specimen tracking? I'm looking for the whole package of cassette printers, work station slide printers, tracking software, etc... > > Any help and recommendations would be appreciated! > > > > Matthew Roark- HT/HTL(ASCP)CM > Histology Specialist > Saint Francis Medical Center > 211 Saint Francis Drive > Cape Girardeau, MO 63703 > 573-331-3982 > mroark@sfmc.net > http://www.sfmc.net > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ From epchatfield <@t> ihis.org Fri Nov 1 09:49:24 2013 From: epchatfield <@t> ihis.org (Elizabeth Chatfield) Date: Fri Nov 1 09:49:35 2013 Subject: [Histonet] Specimen Labeling Message-ID: <52739544020000BC000620E8@gwia-out.peigov> Happy Friday! I'm curious about what the majority of labs accept for specimen labeling, in addition to the usual, ie: name and medical record number/PHN. What is your policy concerning specimen type/source do you require this to be on all specimen containers as well as paper requisitions? Is this a requirement for multiple specimens on the same patient only? or do you require it for single specimen as well? Thank you! Elizabeth ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. From KSimeone <@t> leavittmgt.com Fri Nov 1 10:05:33 2013 From: KSimeone <@t> leavittmgt.com (Delray Beach Pathology Kari Simeone) Date: Fri Nov 1 10:05:46 2013 Subject: [Histonet] RE: Ventana vs. Leica IHC stainers Message-ID: Hi Mehndi. I only use the Leica Bond for my IHC so I cannot give you comparison results/opinions, however, Leica customer service FAR outweighs any other benefit in my experience. It is TOP NOTCH (at least in my region). They bend over backwards for me. I use Ventana Nexus and though their field tech is fantastic, their equipment lacks. That's just the special stainer. Leica also has a HUGE library of RTU antibodies which is great for multiple users and/or new users until they are fully trained. Just my penny's worth! Good luck :-) Kari M Simeone Histology/Immunohistochemistry Specialist Supervisor Alternate Laboratory Supervisor ADCS Clinics, LLC Advanced Dermatology and Cosmetic Surgery Ameriderm ksimeone@leavittmgt.com www.advancedderm.com The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message. From Timothy.Morken <@t> ucsfmedctr.org Fri Nov 1 10:05:34 2013 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Fri Nov 1 10:05:47 2013 Subject: [Histonet] Specimen Tracking Systems In-Reply-To: References: <3e87cff0fdcd4c46bd90911b828a42f5@BLUPR04MB199.namprd04.prod.outlook.com> <761E2B5697F795489C8710BCC72141FF0AE4FF@ex07.net.ucsf.edu> Message-ID: <761E2B5697F795489C8710BCC72141FF0AE601@ex07.net.ucsf.edu> Jesus, the topic of bi-directional interfaces is fraught with both technical and political issues. Technically it is certainly possible (As a software developer colleague of mine always told us - 'don't ask if I can do it, just tell me what you want. It's just software'). The issue is who pays for it, and who wants it. As I see it, the large LIS companies got caught flat-footed by the desire of histology labs to move to barcoding. Indeed, when I worked for a company that manufactured immunostainers we approached several large LIS companies about integrating our bar-code-driven instrument with their systems (we had already prototyped it in our lab with our own mockup of an LIS). I kid you not, the response of each and every one of them was "Why would you want to do that?" Next, they said they could do it if we had a customer that would pay for it. Here in Silicon Valley, where products are developed on the premise that people don't know what they want until you give it to them, that's like admitting you are clueless. Most of these LIS companies are not very proactive and are largely unimaginative in their outlook. That is why Ventana, Leica and others were able to step into that gap and develop their own systems. And at places like U of W they developed their own tracking systems and fully integrated it to their LIS because their LIS did not have anything even planned for barcoding. But now these same LIS companies have finally figured out that that the barcoding ship is leaving the dock and they are scrambling to develop their own systems to compete with the stand-alone systems like Vantage and Cerebro. Due to that competition these LIS companies may not be willing to allow true bi-directional communication - it totally undercuts anything they may develop. They are already at least 5 years behind the curve and can't allow the other companies to gain any further advantage. Tim Morken -----Original Message----- From: Jesus Ellin [mailto:JEllin@yumaregional.org] Sent: Friday, November 01, 2013 6:54 AM To: Morken, Timothy; 'WILLIAM DESALVO'; Matthew D. Roark; histonet Subject: RE: [Histonet] Specimen Tracking Systems I would like to chime in on this Bill and Tim both gave great examples of what it takes to move in this direction. But I would also like to understand why there are so many challenges in getting the data back into the APLIS with updates and orders. I would tell people the biggest concern that I have the data being produced by a system should be accessible across all gradients of the workflow and not just the unique tracking system. Not only is this tracking system positioned to help out histology with what is being explained below, but it is the foundation work in taking steps to get to the next level when talking Digital Pathology, molecular, Personalized medicine, Pathologist cockpit, and algorithm analysis. With the looming cuts coming down the pike, we have to make sure the vendors understand we cannot have barriers when it comes to interface and interoperability with our system. At the same token we cannot be charges an arm, leg and torso for interfacing what is rightfully ours and that is the patients data we produce. Bill and Tim excellent job in giving a crash course in Tracking system 101,, I do believe we should see more of this explained at professional societies meeting and also what the new environments we are going to be working in because of this technology. Just my two cents Jesus Ellin -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Thursday, October 31, 2013 3:45 PM To: 'WILLIAM DESALVO'; Matthew D. Roark; histonet Subject: RE: [Histonet] Specimen Tracking Systems I agree with Bill on this. We have Cerner Copath Plus and looked at Cerebro, Vantage and Omnitrax. Only Cerebro and Vantage were capable of working with Copath. But the problem is that Copath requires you purchase their tracking system to use as an HL-7 interface to any third party system. AND there is not two-way communication between the systems. You MUST enter orders (cases, blocks, stains) in copath and it ports orders to the other system. The only feedback is status updates to copath - you cannot add items in the other system and expect them to show up in copath. That requirement effectively doubled the price of the system. So we are using only the Copath AB&T system, which we are starting to implement now. They will finally have touch screen capability in the spring so we were satisfied with that. Touch screens were a primary spec we demanded. The key to tracking is how the tracking system works with your current LIS. Can it communicate in a true bi-directional fashion? Is it just an add on and all the tracking info is in the tracking component, not the LIS? There are tradeoffs and you need to figure out which you want to live with. We spent two years investigating all these systems, doing site visits, and going through a 6-month total LEAN evaluation. The one thing every institution told us was: LEAN your system BEFORE adding barcoding. Barcoding will NOT fix any inefficiencies you have. And you are pretty much stuck with what ever system you barcode - warts and all. So do all the hard work up front. We thought we were prepared but are daily finding all kinds of little details that have to be worked out. Detail out every single little thing about all your workflows before starting anything. Streamline everything. Cassette printing is the heart of barcoding. The barcoded (actually usually 2D matrix codes) on the cassette drive grossing, tracking through processing, embedding and cutting. If that does not work reliably your system will not work. We went with Leica cassette printers. They are huge, but fast (5 sec per cass). We were going to use thermo, but they had their printhead problem and we dropped the order. They are 20+ seconds per cassttes, tho have a very compact footprint. You will find there are tradeoffs to everything. Slide labeling is the other half of the equation. They must be reliable and survive all histology procedures. We will use thermal transfer label printers rather than direct slide (too expensive- we will have a printer at each microtome- and cumbersome to change out slide types). Slide labeling is a HUGE deal - you have to be sure it works for everything and can be used in your immuno stainers and coverslipping machines. We are using either Brady StainerBondz labels or LabTags Xylituff labels. These were the only ones that had the size we wanted (23 x 19 mm) and survived all processes. General data StainerShield are also good, but only come in 22 x 22 size. Plus are the most expensive. You will find it much easier if you use labels and ribbons from one manufacturer, designed to use with specific printers. That simplifies a lot of the effort. We have tried to mix and match but it is very difficult because all manufacturers do not make matching labels and ribbons for all printers. Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO Sent: Thursday, October 31, 2013 3:01 PM To: Matthew D. Roark; histonet Subject: RE: [Histonet] Specimen Tracking Systems My lab chose the Ventana Vantage System w/ Thermo PrintMate cassette printers. We are a multi-location system and Vantage works well for us. We wanted a robust tracking and flexible quality assurance system and Ventana provided what we needed and wanted. I suggest you look at the big four to start your evaluation: Ventana - Vantage Leica- Cerebro Thermo- OmniTrax General Data - HTS The larger companies have more interfaces built for the different LIS used in Histology. There are many smaller companies that offer tracking systems, but make sure they can connect w/ your LIS. After product selection, support is critical. Sunquest and Cerner Millennium now offer tracking and quality assurance modules. And I imagine there are other tracking systems built into more LIS. You will also want to evaluate multiple printers for your cassettes. Always keep in mind the amount of information you will be adding to the cassette a this will reduce the number of cassettes/ minute. Start w/ the tracking system vendor and see what the have validated. Consider the upkeep and maintenance of the printer. We choose heat transfer, but there are Laser and Ink-jet that work really well. We chose to point of generation and wanted a very small footprint to place the printers in the grossing stations. We also choose not to use a slide printing system and decided the bar coded labels at microtomy was the best solution for us. We wanted less maintenance and steady throughput. Easier to load a roll of labels than keeping a printer up and running. Last, but certainly not least, support. You will need a ton of support to work through this project: interfaces, IT upgrades, software adjustments, WORKFLOW changes and training. Plan and re-plan before you make your selection. And negotiate hard, the pricing is all over the place for the systems. William DeSalvo, BS HTL(ASCP) Production Manager-Anatomic Pathology Chair, NSH Quality Management Committee Board Member, Digital Pathology Association Owner/Consultant, Collaborative Advantage Consulting > From: mroark@sfmc.net > To: histonet@lists.utsouthwestern.edu > Date: Thu, 31 Oct 2013 20:21:47 +0000 > Subject: [Histonet] Specimen Tracking Systems > > What systems are people using for specimen tracking? I'm looking for the whole package of cassette printers, work station slide printers, tracking software, etc... > > Any help and recommendations would be appreciated! > > > > Matthew Roark- HT/HTL(ASCP)CM > Histology Specialist > Saint Francis Medical Center > 211 Saint Francis Drive > Cape Girardeau, MO 63703 > 573-331-3982 > mroark@sfmc.net > http://www.sfmc.net > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ From wdesalvo.cac <@t> outlook.com Fri Nov 1 10:17:38 2013 From: wdesalvo.cac <@t> outlook.com (WILLIAM DESALVO) Date: Fri Nov 1 10:17:44 2013 Subject: [Histonet] Specimen Tracking Systems In-Reply-To: References: <3e87cff0fdcd4c46bd90911b828a42f5@BLUPR04MB199.namprd04.prod.outlook.com>, , <761E2B5697F795489C8710BCC72141FF0AE4FF@ex07.net.ucsf.edu>, Message-ID: I completely agree w/ Jesus and Tim. It is way to difficult to connect our data and move Anatomic Pathology to a level plane w/ the rest of Healthcare. We need to demand from our vendors systems that will to allow us seamless integration and communication of our data. Too often, the solutions for AP are piecemealed together, proprietary and just not up to standard. We need tracking and quality systems that will work with all our equipment and we need equipment that will integrate into all information systems. As we move to delivery of personalized medicine, AP will become one of the major centers of data and information and we cannot afford to spend the time and effort to recreate the wheel every time a lab wants to connect. The technology upgrade we need in AP is tried and true, even old, technology. We need this upgrade now. We need an overhaul of the equipment and processes in AP and we must demand new and useful innovation if we are to meet the demands of the ever changing Healthcare market. I also hope more individuals share their experiences at all the professional societies. We need the vendors to hear of the need from the technical, professional and patient perspectives. We will need to engage w/ our vendors to help them understand our needs. William DeSalvo, BS HTL(ASCP) > From: JEllin@yumaregional.org > To: Timothy.Morken@ucsfmedctr.org; wdesalvo.cac@outlook.com; mroark@sfmc.net; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Specimen Tracking Systems > Date: Fri, 1 Nov 2013 13:54:16 +0000 > > I would like to chime in on this Bill and Tim both gave great examples of what it takes to move in this direction. But I would also like to understand why there are so many challenges in getting the data back into the APLIS with updates and orders. I would tell people the biggest concern that I have the data being produced by a system should be accessible across all gradients of the workflow and not just the unique tracking system. Not only is this tracking system positioned to help out histology with what is being explained below, but it is the foundation work in taking steps to get to the next level when talking Digital Pathology, molecular, Personalized medicine, Pathologist cockpit, and algorithm analysis. > > With the looming cuts coming down the pike, we have to make sure the vendors understand we cannot have barriers when it comes to interface and interoperability with our system. At the same token we cannot be charges an arm, leg and torso for interfacing what is rightfully ours and that is the patients data we produce. > > Bill and Tim excellent job in giving a crash course in Tracking system 101,, I do believe we should see more of this explained at professional societies meeting and also what the new environments we are going to be working in because of this technology. > > Just my two cents > > Jesus Ellin > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy > Sent: Thursday, October 31, 2013 3:45 PM > To: 'WILLIAM DESALVO'; Matthew D. Roark; histonet > Subject: RE: [Histonet] Specimen Tracking Systems > > I agree with Bill on this. We have Cerner Copath Plus and looked at Cerebro, Vantage and Omnitrax. Only Cerebro and Vantage were capable of working with Copath. But the problem is that Copath requires you purchase their tracking system to use as an HL-7 interface to any third party system. AND there is not two-way communication between the systems. You MUST enter orders (cases, blocks, stains) in copath and it ports orders to the other system. The only feedback is status updates to copath - you cannot add items in the other system and expect them to show up in copath. That requirement effectively doubled the price of the system. So we are using only the Copath AB&T system, which we are starting to implement now. They will finally have touch screen capability in the spring so we were satisfied with that. Touch screens were a primary spec we demanded. > > The key to tracking is how the tracking system works with your current LIS. Can it communicate in a true bi-directional fashion? Is it just an add on and all the tracking info is in the tracking component, not the LIS? There are tradeoffs and you need to figure out which you want to live with. > > We spent two years investigating all these systems, doing site visits, and going through a 6-month total LEAN evaluation. The one thing every institution told us was: LEAN your system BEFORE adding barcoding. Barcoding will NOT fix any inefficiencies you have. And you are pretty much stuck with what ever system you barcode - warts and all. So do all the hard work up front. > > We thought we were prepared but are daily finding all kinds of little details that have to be worked out. Detail out every single little thing about all your workflows before starting anything. Streamline everything. > > Cassette printing is the heart of barcoding. The barcoded (actually usually 2D matrix codes) on the cassette drive grossing, tracking through processing, embedding and cutting. If that does not work reliably your system will not work. > > We went with Leica cassette printers. They are huge, but fast (5 sec per cass). We were going to use thermo, but they had their printhead problem and we dropped the order. They are 20+ seconds per cassttes, tho have a very compact footprint. You will find there are tradeoffs to everything. > > Slide labeling is the other half of the equation. They must be reliable and survive all histology procedures. > > We will use thermal transfer label printers rather than direct slide (too expensive- we will have a printer at each microtome- and cumbersome to change out slide types). Slide labeling is a HUGE deal - you have to be sure it works for everything and can be used in your immuno stainers and coverslipping machines. We are using either Brady StainerBondz labels or LabTags Xylituff labels. These were the only ones that had the size we wanted (23 x 19 mm) and survived all processes. General data StainerShield are also good, but only come in 22 x 22 size. Plus are the most expensive. > > You will find it much easier if you use labels and ribbons from one manufacturer, designed to use with specific printers. That simplifies a lot of the effort. We have tried to mix and match but it is very difficult because all manufacturers do not make matching labels and ribbons for all printers. > > > > Tim Morken > Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO > Sent: Thursday, October 31, 2013 3:01 PM > To: Matthew D. Roark; histonet > Subject: RE: [Histonet] Specimen Tracking Systems > > My lab chose the Ventana Vantage System w/ Thermo PrintMate cassette printers. We are a multi-location system and Vantage works well for us. We wanted a robust tracking and flexible quality assurance system and Ventana provided what we needed and wanted. > > I suggest you look at the big four to start your evaluation: > Ventana - Vantage > Leica- Cerebro > Thermo- OmniTrax > General Data - HTS > > The larger companies have more interfaces built for the different LIS used in Histology. There are many smaller companies that offer tracking systems, but make sure they can connect w/ your LIS. After product selection, support is critical. > > Sunquest and Cerner Millennium now offer tracking and quality assurance modules. And I imagine there are other tracking systems built into more LIS. > > You will also want to evaluate multiple printers for your cassettes. Always keep in mind the amount of information you will be adding to the cassette a this will reduce the number of cassettes/ minute. Start w/ the tracking system vendor and see what the have validated. Consider the upkeep and maintenance of the printer. We choose heat transfer, but there are Laser and Ink-jet that work really well. We chose to point of generation and wanted a very small footprint to place the printers in the grossing stations. > > We also choose not to use a slide printing system and decided the bar coded labels at microtomy was the best solution for us. We wanted less maintenance and steady throughput. Easier to load a roll of labels than keeping a printer up and running. > > Last, but certainly not least, support. You will need a ton of support to work through this project: interfaces, IT upgrades, software adjustments, WORKFLOW changes and training. Plan and re-plan before you make your selection. And negotiate hard, the pricing is all over the place for the systems. > > > William DeSalvo, BS HTL(ASCP) > Production Manager-Anatomic Pathology > Chair, NSH Quality Management Committee > Board Member, Digital Pathology Association Owner/Consultant, Collaborative Advantage Consulting > > > > From: mroark@sfmc.net > > To: histonet@lists.utsouthwestern.edu > > Date: Thu, 31 Oct 2013 20:21:47 +0000 > > Subject: [Histonet] Specimen Tracking Systems > > > > What systems are people using for specimen tracking? I'm looking for the whole package of cassette printers, work station slide printers, tracking software, etc... > > > > Any help and recommendations would be appreciated! > > > > > > > > Matthew Roark- HT/HTL(ASCP)CM > > Histology Specialist > > Saint Francis Medical Center > > 211 Saint Francis Drive > > Cape Girardeau, MO 63703 > > 573-331-3982 > > mroark@sfmc.net > > http://www.sfmc.net > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ______________________________________________________________________ > This message is confidential, intended only for the named > recipient(s) and may contain information that is privileged > or exempt from disclosure under applicable law. If you are > not the intended recipient(s), you are notified that the > dissemination, distribution, or copying of this message is > strictly prohibited. If you receive this message in error, > or are not the named recipient(s), please notify the sender > at either the e-mail, fax, address, or telephone number > listed above and delete this e-mail from your computer. > Thank You. > ______________________________________________________________________ From JEllin <@t> yumaregional.org Fri Nov 1 10:35:55 2013 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Fri Nov 1 10:36:06 2013 Subject: [Histonet] Specimen Tracking Systems In-Reply-To: References: <3e87cff0fdcd4c46bd90911b828a42f5@BLUPR04MB199.namprd04.prod.outlook.com>, , <761E2B5697F795489C8710BCC72141FF0AE4FF@ex07.net.ucsf.edu>, Message-ID: Amen to Both Bill and Tim,, I have fought this battle for years and as to put it in Tim's words " The Ship has left the DOCK" but now we MUST demand this,, to stay a float and to move forward this is no longer an option, but rather a integral part of the ship From: WILLIAM DESALVO [mailto:wdesalvo.cac@outlook.com] Sent: Friday, November 01, 2013 8:18 AM To: Jesus Ellin; 'Morken, Timothy'; Matthew D. Roark; histonet Subject: RE: [Histonet] Specimen Tracking Systems I completely agree w/ Jesus and Tim. It is way to difficult to connect our data and move Anatomic Pathology to a level plane w/ the rest of Healthcare. We need to demand from our vendors systems that will to allow us seamless integration and communication of our data. Too often, the solutions for AP are piecemealed together, proprietary and just not up to standard. We need tracking and quality systems that will work with all our equipment and we need equipment that will integrate into all information systems. As we move to delivery of personalized medicine, AP will become one of the major centers of data and information and we cannot afford to spend the time and effort to recreate the wheel every time a lab wants to connect. The technology upgrade we need in AP is tried and true, even old, technology. We need this upgrade now. We need an overhaul of the equipment and processes in AP and we must demand new and useful innovation if we are to meet the demands of the ever changing Healthcare market. I also hope more individuals share their experiences at all the professional societies. We need the vendors to hear of the need from the technical, professional and patient perspectives. We will need to engage w/ our vendors to help them understand our needs. William DeSalvo, BS HTL(ASCP) > From: JEllin@yumaregional.org > To: Timothy.Morken@ucsfmedctr.org; wdesalvo.cac@outlook.com; mroark@sfmc.net; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Specimen Tracking Systems > Date: Fri, 1 Nov 2013 13:54:16 +0000 > > I would like to chime in on this Bill and Tim both gave great examples of what it takes to move in this direction. But I would also like to understand why there are so many challenges in getting the data back into the APLIS with updates and orders. I would tell people the biggest concern that I have the data being produced by a system should be accessible across all gradients of the workflow and not just the unique tracking system. Not only is this tracking system positioned to help out histology with what is being explained below, but it is the foundation work in taking steps to get to the next level when talking Digital Pathology, molecular, Personalized medicine, Pathologist cockpit, and algorithm analysis. > > With the looming cuts coming down the pike, we have to make sure the vendors understand we cannot have barriers when it comes to interface and interoperability with our system. At the same token we cannot be charges an arm, leg and torso for interfacing what is rightfully ours and that is the patients data we produce. > > Bill and Tim excellent job in giving a crash course in Tracking system 101,, I do believe we should see more of this explained at professional societies meeting and also what the new environments we are going to be working in because of this technology. > > Just my two cents > > Jesus Ellin > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy > Sent: Thursday, October 31, 2013 3:45 PM > To: 'WILLIAM DESALVO'; Matthew D. Roark; histonet > Subject: RE: [Histonet] Specimen Tracking Systems > > I agree with Bill on this. We have Cerner Copath Plus and looked at Cerebro, Vantage and Omnitrax. Only Cerebro and Vantage were capable of working with Copath. But the problem is that Copath requires you purchase their tracking system to use as an HL-7 interface to any third party system. AND there is not two-way communication between the systems. You MUST enter orders (cases, blocks, stains) in copath and it ports orders to the other system. The only feedback is status updates to copath - you cannot add items in the other system and expect them to show up in copath. That requirement effectively doubled the price of the system. So we are using only the Copath AB&T system, which we are starting to implement now. They will finally have touch screen capability in the spring so we were satisfied with that. Touch screens were a primary spec we demanded. > > The key to tracking is how the tracking system works with your current LIS. Can it communicate in a true bi-directional fashion? Is it just an add on and all the tracking info is in the tracking component, not the LIS? There are tradeoffs and you need to figure out which you want to live with. > > We spent two years investigating all these systems, doing site visits, and going through a 6-month total LEAN evaluation. The one thing every institution told us was: LEAN your system BEFORE adding barcoding. Barcoding will NOT fix any inefficiencies you have. And you are pretty much stuck with what ever system you barcode - warts and all. So do all the hard work up front. > > We thought we were prepared but are daily finding all kinds of little details that have to be worked out. Detail out every single little thing about all your workflows before starting anything. Streamline everything. > > Cassette printing is the heart of barcoding. The barcoded (actually usually 2D matrix codes) on the cassette drive grossing, tracking through processing, embedding and cutting. If that does not work reliably your system will not work. > > We went with Leica cassette printers. They are huge, but fast (5 sec per cass). We were going to use thermo, but they had their printhead problem and we dropped the order. They are 20+ seconds per cassttes, tho have a very compact footprint. You will find there are tradeoffs to everything. > > Slide labeling is the other half of the equation. They must be reliable and survive all histology procedures. > > We will use thermal transfer label printers rather than direct slide (too expensive- we will have a printer at each microtome- and cumbersome to change out slide types). Slide labeling is a HUGE deal - you have to be sure it works for everything and can be used in your immuno stainers and coverslipping machines. We are using either Brady StainerBondz labels or LabTags Xylituff labels. These were the only ones that had the size we wanted (23 x 19 mm) and survived all processes. General data StainerShield are also good, but only come in 22 x 22 size. Plus are the most expensive. > > You will find it much easier if you use labels and ribbons from one manufacturer, designed to use with specific printers. That simplifies a lot of the effort. We have tried to mix and match but it is very difficult because all manufacturers do not make matching labels and ribbons for all printers. > > > > Tim Morken > Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO > Sent: Thursday, October 31, 2013 3:01 PM > To: Matthew D. Roark; histonet > Subject: RE: [Histonet] Specimen Tracking Systems > > My lab chose the Ventana Vantage System w/ Thermo PrintMate cassette printers. We are a multi-location system and Vantage works well for us. We wanted a robust tracking and flexible quality assurance system and Ventana provided what we needed and wanted. > > I suggest you look at the big four to start your evaluation: > Ventana - Vantage > Leica- Cerebro > Thermo- OmniTrax > General Data - HTS > > The larger companies have more interfaces built for the different LIS used in Histology. There are many smaller companies that offer tracking systems, but make sure they can connect w/ your LIS. After product selection, support is critical. > > Sunquest and Cerner Millennium now offer tracking and quality assurance modules. And I imagine there are other tracking systems built into more LIS. > > You will also want to evaluate multiple printers for your cassettes. Always keep in mind the amount of information you will be adding to the cassette a this will reduce the number of cassettes/ minute. Start w/ the tracking system vendor and see what the have validated. Consider the upkeep and maintenance of the printer. We choose heat transfer, but there are Laser and Ink-jet that work really well. We chose to point of generation and wanted a very small footprint to place the printers in the grossing stations. > > We also choose not to use a slide printing system and decided the bar coded labels at microtomy was the best solution for us. We wanted less maintenance and steady throughput. Easier to load a roll of labels than keeping a printer up and running. > > Last, but certainly not least, support. You will need a ton of support to work through this project: interfaces, IT upgrades, software adjustments, WORKFLOW changes and training. Plan and re-plan before you make your selection. And negotiate hard, the pricing is all over the place for the systems. > > > William DeSalvo, BS HTL(ASCP) > Production Manager-Anatomic Pathology > Chair, NSH Quality Management Committee > Board Member, Digital Pathology Association Owner/Consultant, Collaborative Advantage Consulting > > > > From: mroark@sfmc.net > > To: histonet@lists.utsouthwestern.edu > > Date: Thu, 31 Oct 2013 20:21:47 +0000 > > Subject: [Histonet] Specimen Tracking Systems > > > > What systems are people using for specimen tracking? I'm looking for the whole package of cassette printers, work station slide printers, tracking software, etc... > > > > Any help and recommendations would be appreciated! > > > > > > > > Matthew Roark- HT/HTL(ASCP)CM > > Histology Specialist > > Saint Francis Medical Center > > 211 Saint Francis Drive > > Cape Girardeau, MO 63703 > > 573-331-3982 > > mroark@sfmc.net> > > http://www.sfmc.net> > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ______________________________________________________________________ > This message is confidential, intended only for the named > recipient(s) and may contain information that is privileged > or exempt from disclosure under applicable law. If you are > not the intended recipient(s), you are notified that the > dissemination, distribution, or copying of this message is > strictly prohibited. If you receive this message in error, > or are not the named recipient(s), please notify the sender > at either the e-mail, fax, address, or telephone number > listed above and delete this e-mail from your computer. > Thank You. > ______________________________________________________________________ ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ From Caroline.Pratt <@t> uphs.upenn.edu Fri Nov 1 10:39:49 2013 From: Caroline.Pratt <@t> uphs.upenn.edu (Pratt, Caroline) Date: Fri Nov 1 10:39:54 2013 Subject: [Histonet] Specimen Labeling In-Reply-To: <52739544020000BC000620E8@gwia-out.peigov> References: <52739544020000BC000620E8@gwia-out.peigov> Message-ID: We accept single specimens without a site if the patient's full name and dob match the protocol exactly. Most physicians label the site, but we won't send a single sight case back for that reason. We do request and prefer the site as well. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chatfield Sent: Friday, November 01, 2013 10:49 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Specimen Labeling Happy Friday! I'm curious about what the majority of labs accept for specimen labeling, in addition to the usual, ie: name and medical record number/PHN. What is your policy concerning specimen type/source do you require this to be on all specimen containers as well as paper requisitions? Is this a requirement for multiple specimens on the same patient only? or do you require it for single specimen as well? Thank you! Elizabeth ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From Bauer.Karen <@t> mayo.edu Fri Nov 1 11:02:51 2013 From: Bauer.Karen <@t> mayo.edu (Bauer, Karen L.) Date: Fri Nov 1 11:02:57 2013 Subject: [Histonet] Specimen Tracking Systems In-Reply-To: <761E2B5697F795489C8710BCC72141FF0AE601@ex07.net.ucsf.edu> References: <3e87cff0fdcd4c46bd90911b828a42f5@BLUPR04MB199.namprd04.prod.outlook.com> <761E2B5697F795489C8710BCC72141FF0AE4FF@ex07.net.ucsf.edu> <761E2B5697F795489C8710BCC72141FF0AE601@ex07.net.ucsf.edu> Message-ID: <8A2A8E67E35BB24DAD9375F9E16310750FE7F7@MSGPEXCHA01A.mfad.mfroot.org> Jesus, Tim, and Bill... Wonderful communication about specimen tracking, bi-directional capabilities, and creating a histology lab that will be effective in the years to come!! I loved reading your information! We also have the Vantage system and it's helped us decrease our slide labeling error rate to 0%! It's wonderful and I'm so glad we finally had it implemented last April. Our workflow seems much more efficient, since we no longer have to perform all of those manual reconciliation steps. Yes, the bi-directionality of the systems (we have Sunquest CoPath) is a downfall of the software, but the pros of Vantage outweighed that flaw. I'm hoping that in the years to come, that will be fixed. A new question for the Histo group... We are trying to get away from printed grossing working drafts that are submitted with the slides and delivered to the pathologists. We would like the docs to scan the slide at their microscope and have the patient information show up on their computer. The pathologists still want the paper requisition from the specimen, so I suggested to have the requisition scanned and attached in the digital format. This way, when the doc scans the slide, the CoPath working draft, the patient clinical hx, and the scanned req slip can be viewed. Is anyone doing this in their lab right now? If so, I would really like to hear more about how you and your LIS made that happen. Thanks so much and Happy Friday!!!!! :) Karen Karen L. Bauer HTL/HT (ASCP) | Histology Supervisor | Pathology | MOHS Lab Supervisor | Dermatology | Phone: 715-838-3205 | bauer.karen@mayo.edu | Mayo Clinic Health System | 1221 Whipple Street | Eau Claire, WI 54702 | mayoclinichealthsystem.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Friday, November 01, 2013 10:06 AM To: 'Jesus Ellin'; 'WILLIAM DESALVO'; Matthew D. Roark; histonet Subject: RE: [Histonet] Specimen Tracking Systems Jesus, the topic of bi-directional interfaces is fraught with both technical and political issues. Technically it is certainly possible (As a software developer colleague of mine always told us - 'don't ask if I can do it, just tell me what you want. It's just software'). The issue is who pays for it, and who wants it. As I see it, the large LIS companies got caught flat-footed by the desire of histology labs to move to barcoding. Indeed, when I worked for a company that manufactured immunostainers we approached several large LIS companies about integrating our bar-code-driven instrument with their systems (we had already prototyped it in our lab with our own mockup of an LIS). I kid you not, the response of each and every one of them was "Why would you want to do that?" Next, they said they could do it if we had a customer that would pay for it. Here in Silicon Valley, where products are developed on the premise that people don't know what they want until you give it to them, that's like admitting you are clueless. Most of these LIS companies are not very proactive and are largely unimaginative in their outlook. That is why Ventana, Leica and others were able to step into that gap and develop their own systems. And at places like U of W they developed their own tracking systems and fully integrated it to their LIS because their LIS did not have anything even planned for barcoding. But now these same LIS companies have finally figured out that that the barcoding ship is leaving the dock and they are scrambling to develop their own systems to compete with the stand-alone systems like Vantage and Cerebro. Due to that competition these LIS companies may not be willing to allow true bi-directional communication - it totally undercuts anything they may develop. They are already at least 5 years behind the curve and can't allow the other companies to gain any further advantage. Tim Morken -----Original Message----- From: Jesus Ellin [mailto:JEllin@yumaregional.org] Sent: Friday, November 01, 2013 6:54 AM To: Morken, Timothy; 'WILLIAM DESALVO'; Matthew D. Roark; histonet Subject: RE: [Histonet] Specimen Tracking Systems I would like to chime in on this Bill and Tim both gave great examples of what it takes to move in this direction. But I would also like to understand why there are so many challenges in getting the data back into the APLIS with updates and orders. I would tell people the biggest concern that I have the data being produced by a system should be accessible across all gradients of the workflow and not just the unique tracking system. Not only is this tracking system positioned to help out histology with what is being explained below, but it is the foundation work in taking steps to get to the next level when talking Digital Pathology, molecular, Personalized medicine, Pathologist cockpit, and algorithm analysis. With the looming cuts coming down the pike, we have to make sure the vendors understand we cannot have barriers when it comes to interface and interoperability with our system. At the same token we cannot be charges an arm, leg and torso for interfacing what is rightfully ours and that is the patients data we produce. Bill and Tim excellent job in giving a crash course in Tracking system 101,, I do believe we should see more of this explained at professional societies meeting and also what the new environments we are going to be working in because of this technology. Just my two cents Jesus Ellin -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Thursday, October 31, 2013 3:45 PM To: 'WILLIAM DESALVO'; Matthew D. Roark; histonet Subject: RE: [Histonet] Specimen Tracking Systems I agree with Bill on this. We have Cerner Copath Plus and looked at Cerebro, Vantage and Omnitrax. Only Cerebro and Vantage were capable of working with Copath. But the problem is that Copath requires you purchase their tracking system to use as an HL-7 interface to any third party system. AND there is not two-way communication between the systems. You MUST enter orders (cases, blocks, stains) in copath and it ports orders to the other system. The only feedback is status updates to copath - you cannot add items in the other system and expect them to show up in copath. That requirement effectively doubled the price of the system. So we are using only the Copath AB&T system, which we are starting to implement now. They will finally have touch screen capability in the spring so we were satisfied with that. Touch screens were a primary spec we demanded. The key to tracking is how the tracking system works with your current LIS. Can it communicate in a true bi-directional fashion? Is it just an add on and all the tracking info is in the tracking component, not the LIS? There are tradeoffs and you need to figure out which you want to live with. We spent two years investigating all these systems, doing site visits, and going through a 6-month total LEAN evaluation. The one thing every institution told us was: LEAN your system BEFORE adding barcoding. Barcoding will NOT fix any inefficiencies you have. And you are pretty much stuck with what ever system you barcode - warts and all. So do all the hard work up front. We thought we were prepared but are daily finding all kinds of little details that have to be worked out. Detail out every single little thing about all your workflows before starting anything. Streamline everything. Cassette printing is the heart of barcoding. The barcoded (actually usually 2D matrix codes) on the cassette drive grossing, tracking through processing, embedding and cutting. If that does not work reliably your system will not work. We went with Leica cassette printers. They are huge, but fast (5 sec per cass). We were going to use thermo, but they had their printhead problem and we dropped the order. They are 20+ seconds per cassttes, tho have a very compact footprint. You will find there are tradeoffs to everything. Slide labeling is the other half of the equation. They must be reliable and survive all histology procedures. We will use thermal transfer label printers rather than direct slide (too expensive- we will have a printer at each microtome- and cumbersome to change out slide types). Slide labeling is a HUGE deal - you have to be sure it works for everything and can be used in your immuno stainers and coverslipping machines. We are using either Brady StainerBondz labels or LabTags Xylituff labels. These were the only ones that had the size we wanted (23 x 19 mm) and survived all processes. General data StainerShield are also good, but only come in 22 x 22 size. Plus are the most expensive. You will find it much easier if you use labels and ribbons from one manufacturer, designed to use with specific printers. That simplifies a lot of the effort. We have tried to mix and match but it is very difficult because all manufacturers do not make matching labels and ribbons for all printers. Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO Sent: Thursday, October 31, 2013 3:01 PM To: Matthew D. Roark; histonet Subject: RE: [Histonet] Specimen Tracking Systems My lab chose the Ventana Vantage System w/ Thermo PrintMate cassette printers. We are a multi-location system and Vantage works well for us. We wanted a robust tracking and flexible quality assurance system and Ventana provided what we needed and wanted. I suggest you look at the big four to start your evaluation: Ventana - Vantage Leica- Cerebro Thermo- OmniTrax General Data - HTS The larger companies have more interfaces built for the different LIS used in Histology. There are many smaller companies that offer tracking systems, but make sure they can connect w/ your LIS. After product selection, support is critical. Sunquest and Cerner Millennium now offer tracking and quality assurance modules. And I imagine there are other tracking systems built into more LIS. You will also want to evaluate multiple printers for your cassettes. Always keep in mind the amount of information you will be adding to the cassette a this will reduce the number of cassettes/ minute. Start w/ the tracking system vendor and see what the have validated. Consider the upkeep and maintenance of the printer. We choose heat transfer, but there are Laser and Ink-jet that work really well. We chose to point of generation and wanted a very small footprint to place the printers in the grossing stations. We also choose not to use a slide printing system and decided the bar coded labels at microtomy was the best solution for us. We wanted less maintenance and steady throughput. Easier to load a roll of labels than keeping a printer up and running. Last, but certainly not least, support. You will need a ton of support to work through this project: interfaces, IT upgrades, software adjustments, WORKFLOW changes and training. Plan and re-plan before you make your selection. And negotiate hard, the pricing is all over the place for the systems. William DeSalvo, BS HTL(ASCP) Production Manager-Anatomic Pathology Chair, NSH Quality Management Committee Board Member, Digital Pathology Association Owner/Consultant, Collaborative Advantage Consulting > From: mroark@sfmc.net > To: histonet@lists.utsouthwestern.edu > Date: Thu, 31 Oct 2013 20:21:47 +0000 > Subject: [Histonet] Specimen Tracking Systems > > What systems are people using for specimen tracking? I'm looking for the whole package of cassette printers, work station slide printers, tracking software, etc... > > Any help and recommendations would be appreciated! > > > > Matthew Roark- HT/HTL(ASCP)CM > Histology Specialist > Saint Francis Medical Center > 211 Saint Francis Drive > Cape Girardeau, MO 63703 > 573-331-3982 > mroark@sfmc.net > http://www.sfmc.net > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Fri Nov 1 11:23:21 2013 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Fri Nov 1 11:23:35 2013 Subject: [Histonet] Specimen Tracking Systems In-Reply-To: <8A2A8E67E35BB24DAD9375F9E16310750FE7F7@MSGPEXCHA01A.mfad.mfroot.org> References: <3e87cff0fdcd4c46bd90911b828a42f5@BLUPR04MB199.namprd04.prod.outlook.com> <761E2B5697F795489C8710BCC72141FF0AE4FF@ex07.net.ucsf.edu> <761E2B5697F795489C8710BCC72141FF0AE601@ex07.net.ucsf.edu> <8A2A8E67E35BB24DAD9375F9E16310750FE7F7@MSGPEXCHA01A.mfad.mfroot.org> Message-ID: <761E2B5697F795489C8710BCC72141FF0AE6B0@ex07.net.ucsf.edu> Karen, we are going to move to a system as you describe. At accessioning all paperwork will be scanned and none will follow the specimen. The critical trick is getting residents and pathologists to look at screens rather than hard copies. We spend way too much time printing and collating hard copies of reports ever day. Tim Morken Pathology UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: Bauer, Karen L. [mailto:Bauer.Karen@mayo.edu] Sent: Friday, November 01, 2013 9:03 AM To: Morken, Timothy; 'Jesus Ellin'; 'WILLIAM DESALVO'; 'Matthew D. Roark'; 'histonet' Subject: RE: [Histonet] Specimen Tracking Systems Importance: Low Jesus, Tim, and Bill... Wonderful communication about specimen tracking, bi-directional capabilities, and creating a histology lab that will be effective in the years to come!! I loved reading your information! We also have the Vantage system and it's helped us decrease our slide labeling error rate to 0%! It's wonderful and I'm so glad we finally had it implemented last April. Our workflow seems much more efficient, since we no longer have to perform all of those manual reconciliation steps. Yes, the bi-directionality of the systems (we have Sunquest CoPath) is a downfall of the software, but the pros of Vantage outweighed that flaw. I'm hoping that in the years to come, that will be fixed. A new question for the Histo group... We are trying to get away from printed grossing working drafts that are submitted with the slides and delivered to the pathologists. We would like the docs to scan the slide at their microscope and have the patient information show up on their computer. The pathologists still want the paper requisition from the specimen, so I suggested to have the requisition scanned and attached in the digital format. This way, when the doc scans the slide, the CoPath working draft, the patient clinical hx, and the scanned req slip can be viewed. Is anyone doing this in their lab right now? If so, I would really like to hear more about how you and your LIS made that happen. Thanks so much and Happy Friday!!!!! :) Karen Karen L. Bauer HTL/HT (ASCP) | Histology Supervisor | Pathology | MOHS Lab Supervisor | Dermatology | Phone: 715-838-3205 | bauer.karen@mayo.edu | Mayo Clinic Health System | 1221 Whipple Street | Eau Claire, WI 54702 | mayoclinichealthsystem.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Friday, November 01, 2013 10:06 AM To: 'Jesus Ellin'; 'WILLIAM DESALVO'; Matthew D. Roark; histonet Subject: RE: [Histonet] Specimen Tracking Systems Jesus, the topic of bi-directional interfaces is fraught with both technical and political issues. Technically it is certainly possible (As a software developer colleague of mine always told us - 'don't ask if I can do it, just tell me what you want. It's just software'). The issue is who pays for it, and who wants it. As I see it, the large LIS companies got caught flat-footed by the desire of histology labs to move to barcoding. Indeed, when I worked for a company that manufactured immunostainers we approached several large LIS companies about integrating our bar-code-driven instrument with their systems (we had already prototyped it in our lab with our own mockup of an LIS). I kid you not, the response of each and every one of them was "Why would you want to do that?" Next, they said they could do it if we had a customer that would pay for it. Here in Silicon Valley, where products are developed on the premise that people don't know what they want until you give it to them, that's like admitting you are clueless. Most of these LIS companies are not very proactive and are largely unimaginative in their outlook. That is why Ventana, Leica and others were able to step into that gap and develop their own systems. And at places like U of W they developed their own tracking systems and fully integrated it to their LIS because their LIS did not have anything even planned for barcoding. But now these same LIS companies have finally figured out that that the barcoding ship is leaving the dock and they are scrambling to develop their own systems to compete with the stand-alone systems like Vantage and Cerebro. Due to that competition these LIS companies may not be willing to allow true bi-directional communication - it totally undercuts anything they may develop. They are already at least 5 years behind the curve and can't allow the other companies to gain any further advantage. Tim Morken -----Original Message----- From: Jesus Ellin [mailto:JEllin@yumaregional.org] Sent: Friday, November 01, 2013 6:54 AM To: Morken, Timothy; 'WILLIAM DESALVO'; Matthew D. Roark; histonet Subject: RE: [Histonet] Specimen Tracking Systems I would like to chime in on this Bill and Tim both gave great examples of what it takes to move in this direction. But I would also like to understand why there are so many challenges in getting the data back into the APLIS with updates and orders. I would tell people the biggest concern that I have the data being produced by a system should be accessible across all gradients of the workflow and not just the unique tracking system. Not only is this tracking system positioned to help out histology with what is being explained below, but it is the foundation work in taking steps to get to the next level when talking Digital Pathology, molecular, Personalized medicine, Pathologist cockpit, and algorithm analysis. With the looming cuts coming down the pike, we have to make sure the vendors understand we cannot have barriers when it comes to interface and interoperability with our system. At the same token we cannot be charges an arm, leg and torso for interfacing what is rightfully ours and that is the patients data we produce. Bill and Tim excellent job in giving a crash course in Tracking system 101,, I do believe we should see more of this explained at professional societies meeting and also what the new environments we are going to be working in because of this technology. Just my two cents Jesus Ellin -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Thursday, October 31, 2013 3:45 PM To: 'WILLIAM DESALVO'; Matthew D. Roark; histonet Subject: RE: [Histonet] Specimen Tracking Systems I agree with Bill on this. We have Cerner Copath Plus and looked at Cerebro, Vantage and Omnitrax. Only Cerebro and Vantage were capable of working with Copath. But the problem is that Copath requires you purchase their tracking system to use as an HL-7 interface to any third party system. AND there is not two-way communication between the systems. You MUST enter orders (cases, blocks, stains) in copath and it ports orders to the other system. The only feedback is status updates to copath - you cannot add items in the other system and expect them to show up in copath. That requirement effectively doubled the price of the system. So we are using only the Copath AB&T system, which we are starting to implement now. They will finally have touch screen capability in the spring so we were satisfied with that. Touch screens were a primary spec we demanded. The key to tracking is how the tracking system works with your current LIS. Can it communicate in a true bi-directional fashion? Is it just an add on and all the tracking info is in the tracking component, not the LIS? There are tradeoffs and you need to figure out which you want to live with. We spent two years investigating all these systems, doing site visits, and going through a 6-month total LEAN evaluation. The one thing every institution told us was: LEAN your system BEFORE adding barcoding. Barcoding will NOT fix any inefficiencies you have. And you are pretty much stuck with what ever system you barcode - warts and all. So do all the hard work up front. We thought we were prepared but are daily finding all kinds of little details that have to be worked out. Detail out every single little thing about all your workflows before starting anything. Streamline everything. Cassette printing is the heart of barcoding. The barcoded (actually usually 2D matrix codes) on the cassette drive grossing, tracking through processing, embedding and cutting. If that does not work reliably your system will not work. We went with Leica cassette printers. They are huge, but fast (5 sec per cass). We were going to use thermo, but they had their printhead problem and we dropped the order. They are 20+ seconds per cassttes, tho have a very compact footprint. You will find there are tradeoffs to everything. Slide labeling is the other half of the equation. They must be reliable and survive all histology procedures. We will use thermal transfer label printers rather than direct slide (too expensive- we will have a printer at each microtome- and cumbersome to change out slide types). Slide labeling is a HUGE deal - you have to be sure it works for everything and can be used in your immuno stainers and coverslipping machines. We are using either Brady StainerBondz labels or LabTags Xylituff labels. These were the only ones that had the size we wanted (23 x 19 mm) and survived all processes. General data StainerShield are also good, but only come in 22 x 22 size. Plus are the most expensive. You will find it much easier if you use labels and ribbons from one manufacturer, designed to use with specific printers. That simplifies a lot of the effort. We have tried to mix and match but it is very difficult because all manufacturers do not make matching labels and ribbons for all printers. Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO Sent: Thursday, October 31, 2013 3:01 PM To: Matthew D. Roark; histonet Subject: RE: [Histonet] Specimen Tracking Systems My lab chose the Ventana Vantage System w/ Thermo PrintMate cassette printers. We are a multi-location system and Vantage works well for us. We wanted a robust tracking and flexible quality assurance system and Ventana provided what we needed and wanted. I suggest you look at the big four to start your evaluation: Ventana - Vantage Leica- Cerebro Thermo- OmniTrax General Data - HTS The larger companies have more interfaces built for the different LIS used in Histology. There are many smaller companies that offer tracking systems, but make sure they can connect w/ your LIS. After product selection, support is critical. Sunquest and Cerner Millennium now offer tracking and quality assurance modules. And I imagine there are other tracking systems built into more LIS. You will also want to evaluate multiple printers for your cassettes. Always keep in mind the amount of information you will be adding to the cassette a this will reduce the number of cassettes/ minute. Start w/ the tracking system vendor and see what the have validated. Consider the upkeep and maintenance of the printer. We choose heat transfer, but there are Laser and Ink-jet that work really well. We chose to point of generation and wanted a very small footprint to place the printers in the grossing stations. We also choose not to use a slide printing system and decided the bar coded labels at microtomy was the best solution for us. We wanted less maintenance and steady throughput. Easier to load a roll of labels than keeping a printer up and running. Last, but certainly not least, support. You will need a ton of support to work through this project: interfaces, IT upgrades, software adjustments, WORKFLOW changes and training. Plan and re-plan before you make your selection. And negotiate hard, the pricing is all over the place for the systems. William DeSalvo, BS HTL(ASCP) Production Manager-Anatomic Pathology Chair, NSH Quality Management Committee Board Member, Digital Pathology Association Owner/Consultant, Collaborative Advantage Consulting > From: mroark@sfmc.net > To: histonet@lists.utsouthwestern.edu > Date: Thu, 31 Oct 2013 20:21:47 +0000 > Subject: [Histonet] Specimen Tracking Systems > > What systems are people using for specimen tracking? I'm looking for the whole package of cassette printers, work station slide printers, tracking software, etc... > > Any help and recommendations would be appreciated! > > > > Matthew Roark- HT/HTL(ASCP)CM > Histology Specialist > Saint Francis Medical Center > 211 Saint Francis Drive > Cape Girardeau, MO 63703 > 573-331-3982 > mroark@sfmc.net > http://www.sfmc.net > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mike <@t> pathview.com Fri Nov 1 11:28:30 2013 From: mike <@t> pathview.com (Michael Mihalik) Date: Fri Nov 1 11:28:43 2013 Subject: [Histonet] Specimen Tracking Systems In-Reply-To: References: <3e87cff0fdcd4c46bd90911b828a42f5@BLUPR04MB199.namprd04.prod.outlook.com>, , <761E2B5697F795489C8710BCC72141FF0AE4FF@ex07.net.ucsf.edu>, Message-ID: <005601ced71f$6743aed0$35cb0c70$@pathview.com> I pretty much agree with everything that has been already stated, but I thought I would add the perspective of an LIS vendor to the conversation. If anyone wishes to further discuss any of statements, publicly or privately, I'm more than willing to do so. 1. Tracking systems can work with minimal to no interfaces, but though effective, they can be less than optimal and limited in scope. It's all about the workflow (how easy it is to do something because if it's difficult, it won't happen) and it all depends on your objective. For instance, if all you want to do is gather statistics on personnel and turnaround times for points tracked within your tracking system, then interfaces, especially 'data back to the LIS' are not as necessary. On the other hand, if that is your sole objective, I would counter that there are many more benefits to be gained from a tracking system. Think of this scenario: A tech has found a problem or suspects a problem with a block or slide. She/he documents it in the tracking system, but how does that information get to the pathologist when she/he is looking at the slide? The tracking system has the quality comment and can therefore report out statistics, but that doesn't help the pathologist during the analysis of the slide. 2. Why is it so hard to communicate this data from the LIS to a middleware tracking system and vice versa? In a lot of ways, we've already read the answer. There is no technical reason for this. It's just more software. On the other hand, look at the complexity involved. It's not just a matter of sending information to and from the tracking system, it's a matter of where and how the LIS displays and interacts with the data. By the time, a vendor has truly worked this out in their own system, they have essentially written a tracking system themselves. Furthermore, to truly write a tightly coupled interface means that the LIS vendor is constantly expending effort to match the changes of the tracking system vendor. With this much effort involved, why not build a tracking system within the LIS? 3. To answer my last question, to truly a build a good tracking system, you have to rewrite your LIS from the ground up because tracking and quality can and should permeate all aspects of the workflow process. If a vendor has a certain amount of money to spend on either marketing /sales or on development, what will they do? I would propose that most vendors are now trying to reach some compromise. The vendor won't rewrite extensive parts of their system because that's too expensive, but they will patch some 'piece of software' on to the existing LIS and then use the remaining monies to market/sell their solution. Remember, this is just my opinion. I don't really know what other vendors do. ...but back to the original email. The 30 second bit of advice is this: Define your objectives and then pursue a solution that will help you meet your objectives. If I were in your shoes, I'd prepare my list of objectives by a. looking at the problems in my lab that I'm trying to solve, b. looking at the problems and benefits other people have dealt with (like you're doing now via histonet (thank you again histonet)), and c. looking at the 'features/benefits' that various vendors talk about. Distill a list and go from there, but and this is a big but, try to find some way to factor in the support and relationship you will have with the vendor. Purchasing a computer solution today is truly like being married. Divorces are expensive and an unbalanced or incompatible relationship is painful on a day in, day out basis. Michael Mihalik PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jesus Ellin Sent: Friday, November 01, 2013 8:36 AM To: 'WILLIAM DESALVO'; 'Morken, Timothy'; Matthew D. Roark; histonet Subject: RE: [Histonet] Specimen Tracking Systems Amen to Both Bill and Tim,, I have fought this battle for years and as to put it in Tim's words " The Ship has left the DOCK" but now we MUST demand this,, to stay a float and to move forward this is no longer an option, but rather a integral part of the ship From: WILLIAM DESALVO [mailto:wdesalvo.cac@outlook.com] Sent: Friday, November 01, 2013 8:18 AM To: Jesus Ellin; 'Morken, Timothy'; Matthew D. Roark; histonet Subject: RE: [Histonet] Specimen Tracking Systems I completely agree w/ Jesus and Tim. It is way to difficult to connect our data and move Anatomic Pathology to a level plane w/ the rest of Healthcare. We need to demand from our vendors systems that will to allow us seamless integration and communication of our data. Too often, the solutions for AP are piecemealed together, proprietary and just not up to standard. We need tracking and quality systems that will work with all our equipment and we need equipment that will integrate into all information systems. As we move to delivery of personalized medicine, AP will become one of the major centers of data and information and we cannot afford to spend the time and effort to recreate the wheel every time a lab wants to connect. The technology upgrade we need in AP is tried and true, even old, technology. We need this upgrade now. We need an overhaul of the equipment and processes in AP and we must demand new and useful innovation if we are to meet the demands of the ever changing Healthcare market. I also hope more individuals share their experiences at all the professional societies. We need the vendors to hear of the need from the technical, professional and patient perspectives. We will need to engage w/ our vendors to help them understand our needs. William DeSalvo, BS HTL(ASCP) > From: JEllin@yumaregional.org > To: Timothy.Morken@ucsfmedctr.org; wdesalvo.cac@outlook.com; > mroark@sfmc.net; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Specimen Tracking Systems > Date: Fri, 1 Nov 2013 13:54:16 +0000 > > I would like to chime in on this Bill and Tim both gave great examples of what it takes to move in this direction. But I would also like to understand why there are so many challenges in getting the data back into the APLIS with updates and orders. I would tell people the biggest concern that I have the data being produced by a system should be accessible across all gradients of the workflow and not just the unique tracking system. Not only is this tracking system positioned to help out histology with what is being explained below, but it is the foundation work in taking steps to get to the next level when talking Digital Pathology, molecular, Personalized medicine, Pathologist cockpit, and algorithm analysis. > > With the looming cuts coming down the pike, we have to make sure the vendors understand we cannot have barriers when it comes to interface and interoperability with our system. At the same token we cannot be charges an arm, leg and torso for interfacing what is rightfully ours and that is the patients data we produce. > > Bill and Tim excellent job in giving a crash course in Tracking system 101,, I do believe we should see more of this explained at professional societies meeting and also what the new environments we are going to be working in because of this technology. > > Just my two cents > > Jesus Ellin > > > -----Original Message----- > From: > histonet-bounces@lists.utsouthwestern.edu s.utsouthwestern.edu> > [mailto:histonet-bounces@lists.utsouthwestern.edu] onet-bounces@lists.utsouthwestern.edu]> On Behalf Of Morken, Timothy > Sent: Thursday, October 31, 2013 3:45 PM > To: 'WILLIAM DESALVO'; Matthew D. Roark; histonet > Subject: RE: [Histonet] Specimen Tracking Systems > > I agree with Bill on this. We have Cerner Copath Plus and looked at Cerebro, Vantage and Omnitrax. Only Cerebro and Vantage were capable of working with Copath. But the problem is that Copath requires you purchase their tracking system to use as an HL-7 interface to any third party system. AND there is not two-way communication between the systems. You MUST enter orders (cases, blocks, stains) in copath and it ports orders to the other system. The only feedback is status updates to copath - you cannot add items in the other system and expect them to show up in copath. That requirement effectively doubled the price of the system. So we are using only the Copath AB&T system, which we are starting to implement now. They will finally have touch screen capability in the spring so we were satisfied with that. Touch screens were a primary spec we demanded. > > The key to tracking is how the tracking system works with your current LIS. Can it communicate in a true bi-directional fashion? Is it just an add on and all the tracking info is in the tracking component, not the LIS? There are tradeoffs and you need to figure out which you want to live with. > > We spent two years investigating all these systems, doing site visits, and going through a 6-month total LEAN evaluation. The one thing every institution told us was: LEAN your system BEFORE adding barcoding. Barcoding will NOT fix any inefficiencies you have. And you are pretty much stuck with what ever system you barcode - warts and all. So do all the hard work up front. > > We thought we were prepared but are daily finding all kinds of little details that have to be worked out. Detail out every single little thing about all your workflows before starting anything. Streamline everything. > > Cassette printing is the heart of barcoding. The barcoded (actually usually 2D matrix codes) on the cassette drive grossing, tracking through processing, embedding and cutting. If that does not work reliably your system will not work. > > We went with Leica cassette printers. They are huge, but fast (5 sec per cass). We were going to use thermo, but they had their printhead problem and we dropped the order. They are 20+ seconds per cassttes, tho have a very compact footprint. You will find there are tradeoffs to everything. > > Slide labeling is the other half of the equation. They must be reliable and survive all histology procedures. > > We will use thermal transfer label printers rather than direct slide (too expensive- we will have a printer at each microtome- and cumbersome to change out slide types). Slide labeling is a HUGE deal - you have to be sure it works for everything and can be used in your immuno stainers and coverslipping machines. We are using either Brady StainerBondz labels or LabTags Xylituff labels. These were the only ones that had the size we wanted (23 x 19 mm) and survived all processes. General data StainerShield are also good, but only come in 22 x 22 size. Plus are the most expensive. > > You will find it much easier if you use labels and ribbons from one manufacturer, designed to use with specific printers. That simplifies a lot of the effort. We have tried to mix and match but it is very difficult because all manufacturers do not make matching labels and ribbons for all printers. > > > > Tim Morken > Supervisor, Electron Microscopy and Neuromuscular Special Studies UC > San Francisco Medical Center San Francisco, CA > > -----Original Message----- > From: > histonet-bounces@lists.utsouthwestern.edu s.utsouthwestern.edu> > [mailto:histonet-bounces@lists.utsouthwestern.edu] onet-bounces@lists.utsouthwestern.edu]> On Behalf Of WILLIAM DESALVO > Sent: Thursday, October 31, 2013 3:01 PM > To: Matthew D. Roark; histonet > Subject: RE: [Histonet] Specimen Tracking Systems > > My lab chose the Ventana Vantage System w/ Thermo PrintMate cassette printers. We are a multi-location system and Vantage works well for us. We wanted a robust tracking and flexible quality assurance system and Ventana provided what we needed and wanted. > > I suggest you look at the big four to start your evaluation: > Ventana - Vantage > Leica- Cerebro > Thermo- OmniTrax > General Data - HTS > > The larger companies have more interfaces built for the different LIS used in Histology. There are many smaller companies that offer tracking systems, but make sure they can connect w/ your LIS. After product selection, support is critical. > > Sunquest and Cerner Millennium now offer tracking and quality assurance modules. And I imagine there are other tracking systems built into more LIS. > > You will also want to evaluate multiple printers for your cassettes. Always keep in mind the amount of information you will be adding to the cassette a this will reduce the number of cassettes/ minute. Start w/ the tracking system vendor and see what the have validated. Consider the upkeep and maintenance of the printer. We choose heat transfer, but there are Laser and Ink-jet that work really well. We chose to point of generation and wanted a very small footprint to place the printers in the grossing stations. > > We also choose not to use a slide printing system and decided the bar coded labels at microtomy was the best solution for us. We wanted less maintenance and steady throughput. Easier to load a roll of labels than keeping a printer up and running. > > Last, but certainly not least, support. You will need a ton of support to work through this project: interfaces, IT upgrades, software adjustments, WORKFLOW changes and training. Plan and re-plan before you make your selection. And negotiate hard, the pricing is all over the place for the systems. > > > William DeSalvo, BS HTL(ASCP) > Production Manager-Anatomic Pathology > Chair, NSH Quality Management Committee Board Member, Digital > Pathology Association Owner/Consultant, Collaborative Advantage > Consulting > > > > From: mroark@sfmc.net > > To: > > histonet@lists.utsouthwestern.edu > rn.edu> > > Date: Thu, 31 Oct 2013 20:21:47 +0000 > > Subject: [Histonet] Specimen Tracking Systems > > > > What systems are people using for specimen tracking? I'm looking for the whole package of cassette printers, work station slide printers, tracking software, etc... > > > > Any help and recommendations would be appreciated! > > > > > > > > Matthew Roark- HT/HTL(ASCP)CM > > Histology Specialist > > Saint Francis Medical Center > > 211 Saint Francis Drive > > Cape Girardeau, MO 63703 > > 573-331-3982 > > mroark@sfmc.net > to:mroark@sfmc.net>> > > http://www.sfmc.net > /www.sfmc.net/>> > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > rn.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu .edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu .edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ______________________________________________________________________ > This message is confidential, intended only for the named > recipient(s) and may contain information that is privileged or exempt > from disclosure under applicable law. If you are not the intended > recipient(s), you are notified that the dissemination, distribution, > or copying of this message is strictly prohibited. If you receive this > message in error, or are not the named recipient(s), please notify the > sender at either the e-mail, fax, address, or telephone number listed > above and delete this e-mail from your computer. > Thank You. > ______________________________________________________________________ ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Bauer.Karen <@t> mayo.edu Fri Nov 1 11:46:16 2013 From: Bauer.Karen <@t> mayo.edu (Bauer, Karen L.) Date: Fri Nov 1 11:46:23 2013 Subject: [Histonet] Specimen Tracking Systems In-Reply-To: <761E2B5697F795489C8710BCC72141FF0AE6B0@ex07.net.ucsf.edu> References: <3e87cff0fdcd4c46bd90911b828a42f5@BLUPR04MB199.namprd04.prod.outlook.com> <761E2B5697F795489C8710BCC72141FF0AE4FF@ex07.net.ucsf.edu> <761E2B5697F795489C8710BCC72141FF0AE601@ex07.net.ucsf.edu> <8A2A8E67E35BB24DAD9375F9E16310750FE7F7@MSGPEXCHA01A.mfad.mfroot.org> <761E2B5697F795489C8710BCC72141FF0AE6B0@ex07.net.ucsf.edu> Message-ID: <8A2A8E67E35BB24DAD9375F9E16310750FE930@MSGPEXCHA01A.mfad.mfroot.org> Thanks for the reply Tim! Yes... I agree, and too much paper is wasted, since digital copies all available in the software that we utilize. We have no residents or PA.s in our lab, and the Histotechs probably do 80% of the daily gross. We also do 100% of the accessioning of all surgical specimens. We would be more than happy to get away from using the paper reqs, plus it would keep the paper copies out of the gross room and clean. There is the potential for paper slips to get contaminated when grossing specimens and then they are given to the transcriptionists. This process could be so much better. Karen Karen L. Bauer HTL/HT (ASCP) | Histology Supervisor | Pathology | MOHS Lab Supervisor | Dermatology | Phone: 715-838-3205 | bauer.karen@mayo.edu | Mayo Clinic Health System | 1221 Whipple Street | Eau Claire, WI 54702 | mayoclinichealthsystem.org -----Original Message----- From: Morken, Timothy [mailto:Timothy.Morken@ucsfmedctr.org] Sent: Friday, November 01, 2013 11:23 AM To: Bauer, Karen L.; 'Jesus Ellin'; 'WILLIAM DESALVO'; 'Matthew D. Roark'; 'histonet' Subject: RE: [Histonet] Specimen Tracking Systems Karen, we are going to move to a system as you describe. At accessioning all paperwork will be scanned and none will follow the specimen. The critical trick is getting residents and pathologists to look at screens rather than hard copies. We spend way too much time printing and collating hard copies of reports ever day. Tim Morken Pathology UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: Bauer, Karen L. [mailto:Bauer.Karen@mayo.edu] Sent: Friday, November 01, 2013 9:03 AM To: Morken, Timothy; 'Jesus Ellin'; 'WILLIAM DESALVO'; 'Matthew D. Roark'; 'histonet' Subject: RE: [Histonet] Specimen Tracking Systems Importance: Low Jesus, Tim, and Bill... Wonderful communication about specimen tracking, bi-directional capabilities, and creating a histology lab that will be effective in the years to come!! I loved reading your information! We also have the Vantage system and it's helped us decrease our slide labeling error rate to 0%! It's wonderful and I'm so glad we finally had it implemented last April. Our workflow seems much more efficient, since we no longer have to perform all of those manual reconciliation steps. Yes, the bi-directionality of the systems (we have Sunquest CoPath) is a downfall of the software, but the pros of Vantage outweighed that flaw. I'm hoping that in the years to come, that will be fixed. A new question for the Histo group... We are trying to get away from printed grossing working drafts that are submitted with the slides and delivered to the pathologists. We would like the docs to scan the slide at their microscope and have the patient information show up on their computer. The pathologists still want the paper requisition from the specimen, so I suggested to have the requisition scanned and attached in the digital format. This way, when the doc scans the slide, the CoPath working draft, the patient clinical hx, and the scanned req slip can be viewed. Is anyone doing this in their lab right now? If so, I would really like to hear more about how you and your LIS made that happen. Thanks so much and Happy Friday!!!!! :) Karen Karen L. Bauer HTL/HT (ASCP) | Histology Supervisor | Pathology | MOHS Lab Supervisor | Dermatology | Phone: 715-838-3205 | bauer.karen@mayo.edu | Mayo Clinic Health System | 1221 Whipple Street | Eau Claire, WI 54702 | mayoclinichealthsystem.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Friday, November 01, 2013 10:06 AM To: 'Jesus Ellin'; 'WILLIAM DESALVO'; Matthew D. Roark; histonet Subject: RE: [Histonet] Specimen Tracking Systems Jesus, the topic of bi-directional interfaces is fraught with both technical and political issues. Technically it is certainly possible (As a software developer colleague of mine always told us - 'don't ask if I can do it, just tell me what you want. It's just software'). The issue is who pays for it, and who wants it. As I see it, the large LIS companies got caught flat-footed by the desire of histology labs to move to barcoding. Indeed, when I worked for a company that manufactured immunostainers we approached several large LIS companies about integrating our bar-code-driven instrument with their systems (we had already prototyped it in our lab with our own mockup of an LIS). I kid you not, the response of each and every one of them was "Why would you want to do that?" Next, they said they could do it if we had a customer that would pay for it. Here in Silicon Valley, where products are developed on the premise that people don't know what they want until you give it to them, that's like admitting you are clueless. Most of these LIS companies are not very proactive and are largely unimaginative in their outlook. That is why Ventana, Leica and others were able to step into that gap and develop their own systems. And at places like U of W they developed their own tracking systems and fully integrated it to their LIS because their LIS did not have anything even planned for barcoding. But now these same LIS companies have finally figured out that that the barcoding ship is leaving the dock and they are scrambling to develop their own systems to compete with the stand-alone systems like Vantage and Cerebro. Due to that competition these LIS companies may not be willing to allow true bi-directional communication - it totally undercuts anything they may develop. They are already at least 5 years behind the curve and can't allow the other companies to gain any further advantage. Tim Morken -----Original Message----- From: Jesus Ellin [mailto:JEllin@yumaregional.org] Sent: Friday, November 01, 2013 6:54 AM To: Morken, Timothy; 'WILLIAM DESALVO'; Matthew D. Roark; histonet Subject: RE: [Histonet] Specimen Tracking Systems I would like to chime in on this Bill and Tim both gave great examples of what it takes to move in this direction. But I would also like to understand why there are so many challenges in getting the data back into the APLIS with updates and orders. I would tell people the biggest concern that I have the data being produced by a system should be accessible across all gradients of the workflow and not just the unique tracking system. Not only is this tracking system positioned to help out histology with what is being explained below, but it is the foundation work in taking steps to get to the next level when talking Digital Pathology, molecular, Personalized medicine, Pathologist cockpit, and algorithm analysis. With the looming cuts coming down the pike, we have to make sure the vendors understand we cannot have barriers when it comes to interface and interoperability with our system. At the same token we cannot be charges an arm, leg and torso for interfacing what is rightfully ours and that is the patients data we produce. Bill and Tim excellent job in giving a crash course in Tracking system 101,, I do believe we should see more of this explained at professional societies meeting and also what the new environments we are going to be working in because of this technology. Just my two cents Jesus Ellin -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Thursday, October 31, 2013 3:45 PM To: 'WILLIAM DESALVO'; Matthew D. Roark; histonet Subject: RE: [Histonet] Specimen Tracking Systems I agree with Bill on this. We have Cerner Copath Plus and looked at Cerebro, Vantage and Omnitrax. Only Cerebro and Vantage were capable of working with Copath. But the problem is that Copath requires you purchase their tracking system to use as an HL-7 interface to any third party system. AND there is not two-way communication between the systems. You MUST enter orders (cases, blocks, stains) in copath and it ports orders to the other system. The only feedback is status updates to copath - you cannot add items in the other system and expect them to show up in copath. That requirement effectively doubled the price of the system. So we are using only the Copath AB&T system, which we are starting to implement now. They will finally have touch screen capability in the spring so we were satisfied with that. Touch screens were a primary spec we demanded. The key to tracking is how the tracking system works with your current LIS. Can it communicate in a true bi-directional fashion? Is it just an add on and all the tracking info is in the tracking component, not the LIS? There are tradeoffs and you need to figure out which you want to live with. We spent two years investigating all these systems, doing site visits, and going through a 6-month total LEAN evaluation. The one thing every institution told us was: LEAN your system BEFORE adding barcoding. Barcoding will NOT fix any inefficiencies you have. And you are pretty much stuck with what ever system you barcode - warts and all. So do all the hard work up front. We thought we were prepared but are daily finding all kinds of little details that have to be worked out. Detail out every single little thing about all your workflows before starting anything. Streamline everything. Cassette printing is the heart of barcoding. The barcoded (actually usually 2D matrix codes) on the cassette drive grossing, tracking through processing, embedding and cutting. If that does not work reliably your system will not work. We went with Leica cassette printers. They are huge, but fast (5 sec per cass). We were going to use thermo, but they had their printhead problem and we dropped the order. They are 20+ seconds per cassttes, tho have a very compact footprint. You will find there are tradeoffs to everything. Slide labeling is the other half of the equation. They must be reliable and survive all histology procedures. We will use thermal transfer label printers rather than direct slide (too expensive- we will have a printer at each microtome- and cumbersome to change out slide types). Slide labeling is a HUGE deal - you have to be sure it works for everything and can be used in your immuno stainers and coverslipping machines. We are using either Brady StainerBondz labels or LabTags Xylituff labels. These were the only ones that had the size we wanted (23 x 19 mm) and survived all processes. General data StainerShield are also good, but only come in 22 x 22 size. Plus are the most expensive. You will find it much easier if you use labels and ribbons from one manufacturer, designed to use with specific printers. That simplifies a lot of the effort. We have tried to mix and match but it is very difficult because all manufacturers do not make matching labels and ribbons for all printers. Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO Sent: Thursday, October 31, 2013 3:01 PM To: Matthew D. Roark; histonet Subject: RE: [Histonet] Specimen Tracking Systems My lab chose the Ventana Vantage System w/ Thermo PrintMate cassette printers. We are a multi-location system and Vantage works well for us. We wanted a robust tracking and flexible quality assurance system and Ventana provided what we needed and wanted. I suggest you look at the big four to start your evaluation: Ventana - Vantage Leica- Cerebro Thermo- OmniTrax General Data - HTS The larger companies have more interfaces built for the different LIS used in Histology. There are many smaller companies that offer tracking systems, but make sure they can connect w/ your LIS. After product selection, support is critical. Sunquest and Cerner Millennium now offer tracking and quality assurance modules. And I imagine there are other tracking systems built into more LIS. You will also want to evaluate multiple printers for your cassettes. Always keep in mind the amount of information you will be adding to the cassette a this will reduce the number of cassettes/ minute. Start w/ the tracking system vendor and see what the have validated. Consider the upkeep and maintenance of the printer. We choose heat transfer, but there are Laser and Ink-jet that work really well. We chose to point of generation and wanted a very small footprint to place the printers in the grossing stations. We also choose not to use a slide printing system and decided the bar coded labels at microtomy was the best solution for us. We wanted less maintenance and steady throughput. Easier to load a roll of labels than keeping a printer up and running. Last, but certainly not least, support. You will need a ton of support to work through this project: interfaces, IT upgrades, software adjustments, WORKFLOW changes and training. Plan and re-plan before you make your selection. And negotiate hard, the pricing is all over the place for the systems. William DeSalvo, BS HTL(ASCP) Production Manager-Anatomic Pathology Chair, NSH Quality Management Committee Board Member, Digital Pathology Association Owner/Consultant, Collaborative Advantage Consulting > From: mroark@sfmc.net > To: histonet@lists.utsouthwestern.edu > Date: Thu, 31 Oct 2013 20:21:47 +0000 > Subject: [Histonet] Specimen Tracking Systems > > What systems are people using for specimen tracking? I'm looking for the whole package of cassette printers, work station slide printers, tracking software, etc... > > Any help and recommendations would be appreciated! > > > > Matthew Roark- HT/HTL(ASCP)CM > Histology Specialist > Saint Francis Medical Center > 211 Saint Francis Drive > Cape Girardeau, MO 63703 > 573-331-3982 > mroark@sfmc.net > http://www.sfmc.net > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mike <@t> pathview.com Fri Nov 1 11:47:09 2013 From: mike <@t> pathview.com (Michael Mihalik) Date: Fri Nov 1 11:47:27 2013 Subject: [Histonet] Specimen Tracking Systems In-Reply-To: <761E2B5697F795489C8710BCC72141FF0AE6B0@ex07.net.ucsf.edu> References: <3e87cff0fdcd4c46bd90911b828a42f5@BLUPR04MB199.namprd04.prod.outlook.com> <761E2B5697F795489C8710BCC72141FF0AE4FF@ex07.net.ucsf.edu> <761E2B5697F795489C8710BCC72141FF0AE601@ex07.net.ucsf.edu> <8A2A8E67E35BB24DAD9375F9E16310750FE7F7@MSGPEXCHA01A.mfad.mfroot.org> <761E2B5697F795489C8710BCC72141FF0AE6B0@ex07.net.ucsf.edu> Message-ID: <006701ced722$023b1750$06b145f0$@pathview.com> Just a quick thought.... If you scan your requisition into the LIS after accessioning, it might not be a bad idea to have your grossing personnel review the requisition at grossing to confirm that the patient name/specimen on the paperwork matches what's in the LIS. Yes, with barcodes this shouldn't happen, but it's a nice double check. Michael Mihalik PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Friday, November 01, 2013 9:23 AM To: 'Bauer, Karen L.'; 'Jesus Ellin'; 'WILLIAM DESALVO'; 'Matthew D. Roark'; 'histonet' Subject: RE: [Histonet] Specimen Tracking Systems Karen, we are going to move to a system as you describe. At accessioning all paperwork will be scanned and none will follow the specimen. The critical trick is getting residents and pathologists to look at screens rather than hard copies. We spend way too much time printing and collating hard copies of reports ever day. Tim Morken Pathology UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: Bauer, Karen L. [mailto:Bauer.Karen@mayo.edu] Sent: Friday, November 01, 2013 9:03 AM To: Morken, Timothy; 'Jesus Ellin'; 'WILLIAM DESALVO'; 'Matthew D. Roark'; 'histonet' Subject: RE: [Histonet] Specimen Tracking Systems Importance: Low Jesus, Tim, and Bill... Wonderful communication about specimen tracking, bi-directional capabilities, and creating a histology lab that will be effective in the years to come!! I loved reading your information! We also have the Vantage system and it's helped us decrease our slide labeling error rate to 0%! It's wonderful and I'm so glad we finally had it implemented last April. Our workflow seems much more efficient, since we no longer have to perform all of those manual reconciliation steps. Yes, the bi-directionality of the systems (we have Sunquest CoPath) is a downfall of the software, but the pros of Vantage outweighed that flaw. I'm hoping that in the years to come, that will be fixed. A new question for the Histo group... We are trying to get away from printed grossing working drafts that are submitted with the slides and delivered to the pathologists. We would like the docs to scan the slide at their microscope and have the patient information show up on their computer. The pathologists still want the paper requisition from the specimen, so I suggested to have the requisition scanned and attached in the digital format. This way, when the doc scans the slide, the CoPath working draft, the patient clinical hx, and the scanned req slip can be viewed. Is anyone doing this in their lab right now? If so, I would really like to hear more about how you and your LIS made that happen. Thanks so much and Happy Friday!!!!! :) Karen Karen L. Bauer HTL/HT (ASCP) | Histology Supervisor | Pathology | MOHS Lab Supervisor | Dermatology | Phone: 715-838-3205 | bauer.karen@mayo.edu | Mayo Clinic Health System | 1221 Whipple Street | Eau Claire, WI 54702 | mayoclinichealthsystem.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Friday, November 01, 2013 10:06 AM To: 'Jesus Ellin'; 'WILLIAM DESALVO'; Matthew D. Roark; histonet Subject: RE: [Histonet] Specimen Tracking Systems Jesus, the topic of bi-directional interfaces is fraught with both technical and political issues. Technically it is certainly possible (As a software developer colleague of mine always told us - 'don't ask if I can do it, just tell me what you want. It's just software'). The issue is who pays for it, and who wants it. As I see it, the large LIS companies got caught flat-footed by the desire of histology labs to move to barcoding. Indeed, when I worked for a company that manufactured immunostainers we approached several large LIS companies about integrating our bar-code-driven instrument with their systems (we had already prototyped it in our lab with our own mockup of an LIS). I kid you not, the response of each and every one of them was "Why would you want to do that?" Next, they said they could do it if we had a customer that would pay for it. Here in Silicon Valley, where products are developed on the premise that people don't know what they want until you give it to them, that's like admitting you are clueless. Most of these LIS companies are not very proactive and are largely unimaginative in their outlook. That is why Ventana, Leica and others were able to step into that gap and develop their own systems. And at places like U of W they developed their own tracking systems and fully integrated it to their LIS because their LIS did not have anything even planned for barcoding. But now these same LIS companies have finally figured out that that the barcoding ship is leaving the dock and they are scrambling to develop their own systems to compete with the stand-alone systems like Vantage and Cerebro. Due to that competition these LIS companies may not be willing to allow true bi-directional communication - it totally undercuts anything they may develop. They are already at least 5 years behind the curve and can't allow the other companies to gain any further advantage. Tim Morken -----Original Message----- From: Jesus Ellin [mailto:JEllin@yumaregional.org] Sent: Friday, November 01, 2013 6:54 AM To: Morken, Timothy; 'WILLIAM DESALVO'; Matthew D. Roark; histonet Subject: RE: [Histonet] Specimen Tracking Systems I would like to chime in on this Bill and Tim both gave great examples of what it takes to move in this direction. But I would also like to understand why there are so many challenges in getting the data back into the APLIS with updates and orders. I would tell people the biggest concern that I have the data being produced by a system should be accessible across all gradients of the workflow and not just the unique tracking system. Not only is this tracking system positioned to help out histology with what is being explained below, but it is the foundation work in taking steps to get to the next level when talking Digital Pathology, molecular, Personalized medicine, Pathologist cockpit, and algorithm analysis. With the looming cuts coming down the pike, we have to make sure the vendors understand we cannot have barriers when it comes to interface and interoperability with our system. At the same token we cannot be charges an arm, leg and torso for interfacing what is rightfully ours and that is the patients data we produce. Bill and Tim excellent job in giving a crash course in Tracking system 101,, I do believe we should see more of this explained at professional societies meeting and also what the new environments we are going to be working in because of this technology. Just my two cents Jesus Ellin -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Thursday, October 31, 2013 3:45 PM To: 'WILLIAM DESALVO'; Matthew D. Roark; histonet Subject: RE: [Histonet] Specimen Tracking Systems I agree with Bill on this. We have Cerner Copath Plus and looked at Cerebro, Vantage and Omnitrax. Only Cerebro and Vantage were capable of working with Copath. But the problem is that Copath requires you purchase their tracking system to use as an HL-7 interface to any third party system. AND there is not two-way communication between the systems. You MUST enter orders (cases, blocks, stains) in copath and it ports orders to the other system. The only feedback is status updates to copath - you cannot add items in the other system and expect them to show up in copath. That requirement effectively doubled the price of the system. So we are using only the Copath AB&T system, which we are starting to implement now. They will finally have touch screen capability in the spring so we were satisfied with that. Touch screens were a primary spec we demanded. The key to tracking is how the tracking system works with your current LIS. Can it communicate in a true bi-directional fashion? Is it just an add on and all the tracking info is in the tracking component, not the LIS? There are tradeoffs and you need to figure out which you want to live with. We spent two years investigating all these systems, doing site visits, and going through a 6-month total LEAN evaluation. The one thing every institution told us was: LEAN your system BEFORE adding barcoding. Barcoding will NOT fix any inefficiencies you have. And you are pretty much stuck with what ever system you barcode - warts and all. So do all the hard work up front. We thought we were prepared but are daily finding all kinds of little details that have to be worked out. Detail out every single little thing about all your workflows before starting anything. Streamline everything. Cassette printing is the heart of barcoding. The barcoded (actually usually 2D matrix codes) on the cassette drive grossing, tracking through processing, embedding and cutting. If that does not work reliably your system will not work. We went with Leica cassette printers. They are huge, but fast (5 sec per cass). We were going to use thermo, but they had their printhead problem and we dropped the order. They are 20+ seconds per cassttes, tho have a very compact footprint. You will find there are tradeoffs to everything. Slide labeling is the other half of the equation. They must be reliable and survive all histology procedures. We will use thermal transfer label printers rather than direct slide (too expensive- we will have a printer at each microtome- and cumbersome to change out slide types). Slide labeling is a HUGE deal - you have to be sure it works for everything and can be used in your immuno stainers and coverslipping machines. We are using either Brady StainerBondz labels or LabTags Xylituff labels. These were the only ones that had the size we wanted (23 x 19 mm) and survived all processes. General data StainerShield are also good, but only come in 22 x 22 size. Plus are the most expensive. You will find it much easier if you use labels and ribbons from one manufacturer, designed to use with specific printers. That simplifies a lot of the effort. We have tried to mix and match but it is very difficult because all manufacturers do not make matching labels and ribbons for all printers. Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO Sent: Thursday, October 31, 2013 3:01 PM To: Matthew D. Roark; histonet Subject: RE: [Histonet] Specimen Tracking Systems My lab chose the Ventana Vantage System w/ Thermo PrintMate cassette printers. We are a multi-location system and Vantage works well for us. We wanted a robust tracking and flexible quality assurance system and Ventana provided what we needed and wanted. I suggest you look at the big four to start your evaluation: Ventana - Vantage Leica- Cerebro Thermo- OmniTrax General Data - HTS The larger companies have more interfaces built for the different LIS used in Histology. There are many smaller companies that offer tracking systems, but make sure they can connect w/ your LIS. After product selection, support is critical. Sunquest and Cerner Millennium now offer tracking and quality assurance modules. And I imagine there are other tracking systems built into more LIS. You will also want to evaluate multiple printers for your cassettes. Always keep in mind the amount of information you will be adding to the cassette a this will reduce the number of cassettes/ minute. Start w/ the tracking system vendor and see what the have validated. Consider the upkeep and maintenance of the printer. We choose heat transfer, but there are Laser and Ink-jet that work really well. We chose to point of generation and wanted a very small footprint to place the printers in the grossing stations. We also choose not to use a slide printing system and decided the bar coded labels at microtomy was the best solution for us. We wanted less maintenance and steady throughput. Easier to load a roll of labels than keeping a printer up and running. Last, but certainly not least, support. You will need a ton of support to work through this project: interfaces, IT upgrades, software adjustments, WORKFLOW changes and training. Plan and re-plan before you make your selection. And negotiate hard, the pricing is all over the place for the systems. William DeSalvo, BS HTL(ASCP) Production Manager-Anatomic Pathology Chair, NSH Quality Management Committee Board Member, Digital Pathology Association Owner/Consultant, Collaborative Advantage Consulting > From: mroark@sfmc.net > To: histonet@lists.utsouthwestern.edu > Date: Thu, 31 Oct 2013 20:21:47 +0000 > Subject: [Histonet] Specimen Tracking Systems > > What systems are people using for specimen tracking? I'm looking for the whole package of cassette printers, work station slide printers, tracking software, etc... > > Any help and recommendations would be appreciated! > > > > Matthew Roark- HT/HTL(ASCP)CM > Histology Specialist > Saint Francis Medical Center > 211 Saint Francis Drive > Cape Girardeau, MO 63703 > 573-331-3982 > mroark@sfmc.net > http://www.sfmc.net > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Fri Nov 1 11:49:58 2013 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Fri Nov 1 11:50:10 2013 Subject: [Histonet] Specimen Tracking Systems In-Reply-To: <006701ced722$023b1750$06b145f0$@pathview.com> References: <3e87cff0fdcd4c46bd90911b828a42f5@BLUPR04MB199.namprd04.prod.outlook.com> <761E2B5697F795489C8710BCC72141FF0AE4FF@ex07.net.ucsf.edu> <761E2B5697F795489C8710BCC72141FF0AE601@ex07.net.ucsf.edu> <8A2A8E67E35BB24DAD9375F9E16310750FE7F7@MSGPEXCHA01A.mfad.mfroot.org> <761E2B5697F795489C8710BCC72141FF0AE6B0@ex07.net.ucsf.edu> <006701ced722$023b1750$06b145f0$@pathview.com> Message-ID: <761E2B5697F795489C8710BCC72141FF0AE72A@ex07.net.ucsf.edu> Michael, yes that is the sort of workflow item that has to be considered. We plan to use the scanned image at the gross bench to double check. Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: Michael Mihalik [mailto:mike@pathview.com] Sent: Friday, November 01, 2013 9:47 AM To: Morken, Timothy; 'Bauer, Karen L.'; 'Jesus Ellin'; 'WILLIAM DESALVO'; 'Matthew D. Roark'; 'histonet' Subject: RE: [Histonet] Specimen Tracking Systems Just a quick thought.... If you scan your requisition into the LIS after accessioning, it might not be a bad idea to have your grossing personnel review the requisition at grossing to confirm that the patient name/specimen on the paperwork matches what's in the LIS. Yes, with barcodes this shouldn't happen, but it's a nice double check. Michael Mihalik PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Friday, November 01, 2013 9:23 AM To: 'Bauer, Karen L.'; 'Jesus Ellin'; 'WILLIAM DESALVO'; 'Matthew D. Roark'; 'histonet' Subject: RE: [Histonet] Specimen Tracking Systems Karen, we are going to move to a system as you describe. At accessioning all paperwork will be scanned and none will follow the specimen. The critical trick is getting residents and pathologists to look at screens rather than hard copies. We spend way too much time printing and collating hard copies of reports ever day. Tim Morken Pathology UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: Bauer, Karen L. [mailto:Bauer.Karen@mayo.edu] Sent: Friday, November 01, 2013 9:03 AM To: Morken, Timothy; 'Jesus Ellin'; 'WILLIAM DESALVO'; 'Matthew D. Roark'; 'histonet' Subject: RE: [Histonet] Specimen Tracking Systems Importance: Low Jesus, Tim, and Bill... Wonderful communication about specimen tracking, bi-directional capabilities, and creating a histology lab that will be effective in the years to come!! I loved reading your information! We also have the Vantage system and it's helped us decrease our slide labeling error rate to 0%! It's wonderful and I'm so glad we finally had it implemented last April. Our workflow seems much more efficient, since we no longer have to perform all of those manual reconciliation steps. Yes, the bi-directionality of the systems (we have Sunquest CoPath) is a downfall of the software, but the pros of Vantage outweighed that flaw. I'm hoping that in the years to come, that will be fixed. A new question for the Histo group... We are trying to get away from printed grossing working drafts that are submitted with the slides and delivered to the pathologists. We would like the docs to scan the slide at their microscope and have the patient information show up on their computer. The pathologists still want the paper requisition from the specimen, so I suggested to have the requisition scanned and attached in the digital format. This way, when the doc scans the slide, the CoPath working draft, the patient clinical hx, and the scanned req slip can be viewed. Is anyone doing this in their lab right now? If so, I would really like to hear more about how you and your LIS made that happen. Thanks so much and Happy Friday!!!!! :) Karen Karen L. Bauer HTL/HT (ASCP) | Histology Supervisor | Pathology | MOHS Lab Supervisor | Dermatology | Phone: 715-838-3205 | bauer.karen@mayo.edu | Mayo Clinic Health System | 1221 Whipple Street | Eau Claire, WI 54702 | mayoclinichealthsystem.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Friday, November 01, 2013 10:06 AM To: 'Jesus Ellin'; 'WILLIAM DESALVO'; Matthew D. Roark; histonet Subject: RE: [Histonet] Specimen Tracking Systems Jesus, the topic of bi-directional interfaces is fraught with both technical and political issues. Technically it is certainly possible (As a software developer colleague of mine always told us - 'don't ask if I can do it, just tell me what you want. It's just software'). The issue is who pays for it, and who wants it. As I see it, the large LIS companies got caught flat-footed by the desire of histology labs to move to barcoding. Indeed, when I worked for a company that manufactured immunostainers we approached several large LIS companies about integrating our bar-code-driven instrument with their systems (we had already prototyped it in our lab with our own mockup of an LIS). I kid you not, the response of each and every one of them was "Why would you want to do that?" Next, they said they could do it if we had a customer that would pay for it. Here in Silicon Valley, where products are developed on the premise that people don't know what they want until you give it to them, that's like admitting you are clueless. Most of these LIS companies are not very proactive and are largely unimaginative in their outlook. That is why Ventana, Leica and others were able to step into that gap and develop their own systems. And at places like U of W they developed their own tracking systems and fully integrated it to their LIS because their LIS did not have anything even planned for barcoding. But now these same LIS companies have finally figured out that that the barcoding ship is leaving the dock and they are scrambling to develop their own systems to compete with the stand-alone systems like Vantage and Cerebro. Due to that competition these LIS companies may not be willing to allow true bi-directional communication - it totally undercuts anything they may develop. They are already at least 5 years behind the curve and can't allow the other companies to gain any further advantage. Tim Morken -----Original Message----- From: Jesus Ellin [mailto:JEllin@yumaregional.org] Sent: Friday, November 01, 2013 6:54 AM To: Morken, Timothy; 'WILLIAM DESALVO'; Matthew D. Roark; histonet Subject: RE: [Histonet] Specimen Tracking Systems I would like to chime in on this Bill and Tim both gave great examples of what it takes to move in this direction. But I would also like to understand why there are so many challenges in getting the data back into the APLIS with updates and orders. I would tell people the biggest concern that I have the data being produced by a system should be accessible across all gradients of the workflow and not just the unique tracking system. Not only is this tracking system positioned to help out histology with what is being explained below, but it is the foundation work in taking steps to get to the next level when talking Digital Pathology, molecular, Personalized medicine, Pathologist cockpit, and algorithm analysis. With the looming cuts coming down the pike, we have to make sure the vendors understand we cannot have barriers when it comes to interface and interoperability with our system. At the same token we cannot be charges an arm, leg and torso for interfacing what is rightfully ours and that is the patients data we produce. Bill and Tim excellent job in giving a crash course in Tracking system 101,, I do believe we should see more of this explained at professional societies meeting and also what the new environments we are going to be working in because of this technology. Just my two cents Jesus Ellin -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Thursday, October 31, 2013 3:45 PM To: 'WILLIAM DESALVO'; Matthew D. Roark; histonet Subject: RE: [Histonet] Specimen Tracking Systems I agree with Bill on this. We have Cerner Copath Plus and looked at Cerebro, Vantage and Omnitrax. Only Cerebro and Vantage were capable of working with Copath. But the problem is that Copath requires you purchase their tracking system to use as an HL-7 interface to any third party system. AND there is not two-way communication between the systems. You MUST enter orders (cases, blocks, stains) in copath and it ports orders to the other system. The only feedback is status updates to copath - you cannot add items in the other system and expect them to show up in copath. That requirement effectively doubled the price of the system. So we are using only the Copath AB&T system, which we are starting to implement now. They will finally have touch screen capability in the spring so we were satisfied with that. Touch screens were a primary spec we demanded. The key to tracking is how the tracking system works with your current LIS. Can it communicate in a true bi-directional fashion? Is it just an add on and all the tracking info is in the tracking component, not the LIS? There are tradeoffs and you need to figure out which you want to live with. We spent two years investigating all these systems, doing site visits, and going through a 6-month total LEAN evaluation. The one thing every institution told us was: LEAN your system BEFORE adding barcoding. Barcoding will NOT fix any inefficiencies you have. And you are pretty much stuck with what ever system you barcode - warts and all. So do all the hard work up front. We thought we were prepared but are daily finding all kinds of little details that have to be worked out. Detail out every single little thing about all your workflows before starting anything. Streamline everything. Cassette printing is the heart of barcoding. The barcoded (actually usually 2D matrix codes) on the cassette drive grossing, tracking through processing, embedding and cutting. If that does not work reliably your system will not work. We went with Leica cassette printers. They are huge, but fast (5 sec per cass). We were going to use thermo, but they had their printhead problem and we dropped the order. They are 20+ seconds per cassttes, tho have a very compact footprint. You will find there are tradeoffs to everything. Slide labeling is the other half of the equation. They must be reliable and survive all histology procedures. We will use thermal transfer label printers rather than direct slide (too expensive- we will have a printer at each microtome- and cumbersome to change out slide types). Slide labeling is a HUGE deal - you have to be sure it works for everything and can be used in your immuno stainers and coverslipping machines. We are using either Brady StainerBondz labels or LabTags Xylituff labels. These were the only ones that had the size we wanted (23 x 19 mm) and survived all processes. General data StainerShield are also good, but only come in 22 x 22 size. Plus are the most expensive. You will find it much easier if you use labels and ribbons from one manufacturer, designed to use with specific printers. That simplifies a lot of the effort. We have tried to mix and match but it is very difficult because all manufacturers do not make matching labels and ribbons for all printers. Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO Sent: Thursday, October 31, 2013 3:01 PM To: Matthew D. Roark; histonet Subject: RE: [Histonet] Specimen Tracking Systems My lab chose the Ventana Vantage System w/ Thermo PrintMate cassette printers. We are a multi-location system and Vantage works well for us. We wanted a robust tracking and flexible quality assurance system and Ventana provided what we needed and wanted. I suggest you look at the big four to start your evaluation: Ventana - Vantage Leica- Cerebro Thermo- OmniTrax General Data - HTS The larger companies have more interfaces built for the different LIS used in Histology. There are many smaller companies that offer tracking systems, but make sure they can connect w/ your LIS. After product selection, support is critical. Sunquest and Cerner Millennium now offer tracking and quality assurance modules. And I imagine there are other tracking systems built into more LIS. You will also want to evaluate multiple printers for your cassettes. Always keep in mind the amount of information you will be adding to the cassette a this will reduce the number of cassettes/ minute. Start w/ the tracking system vendor and see what the have validated. Consider the upkeep and maintenance of the printer. We choose heat transfer, but there are Laser and Ink-jet that work really well. We chose to point of generation and wanted a very small footprint to place the printers in the grossing stations. We also choose not to use a slide printing system and decided the bar coded labels at microtomy was the best solution for us. We wanted less maintenance and steady throughput. Easier to load a roll of labels than keeping a printer up and running. Last, but certainly not least, support. You will need a ton of support to work through this project: interfaces, IT upgrades, software adjustments, WORKFLOW changes and training. Plan and re-plan before you make your selection. And negotiate hard, the pricing is all over the place for the systems. William DeSalvo, BS HTL(ASCP) Production Manager-Anatomic Pathology Chair, NSH Quality Management Committee Board Member, Digital Pathology Association Owner/Consultant, Collaborative Advantage Consulting > From: mroark@sfmc.net > To: histonet@lists.utsouthwestern.edu > Date: Thu, 31 Oct 2013 20:21:47 +0000 > Subject: [Histonet] Specimen Tracking Systems > > What systems are people using for specimen tracking? I'm looking for > the whole package of cassette printers, work station slide printers, tracking software, etc... > > Any help and recommendations would be appreciated! > > > > Matthew Roark- HT/HTL(ASCP)CM > Histology Specialist > Saint Francis Medical Center > 211 Saint Francis Drive > Cape Girardeau, MO 63703 > 573-331-3982 > mroark@sfmc.net > http://www.sfmc.net > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JEllin <@t> yumaregional.org Fri Nov 1 11:53:35 2013 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Fri Nov 1 11:53:44 2013 Subject: [Histonet] Specimen Tracking Systems In-Reply-To: <761E2B5697F795489C8710BCC72141FF0AE72A@ex07.net.ucsf.edu> References: <3e87cff0fdcd4c46bd90911b828a42f5@BLUPR04MB199.namprd04.prod.outlook.com> <761E2B5697F795489C8710BCC72141FF0AE4FF@ex07.net.ucsf.edu> <761E2B5697F795489C8710BCC72141FF0AE601@ex07.net.ucsf.edu> <8A2A8E67E35BB24DAD9375F9E16310750FE7F7@MSGPEXCHA01A.mfad.mfroot.org> <761E2B5697F795489C8710BCC72141FF0AE6B0@ex07.net.ucsf.edu> <006701ced722$023b1750$06b145f0$@pathview.com> <761E2B5697F795489C8710BCC72141FF0AE72A@ex07.net.ucsf.edu> Message-ID: We scan in at the point of accessioning and do one piece flow. As Tim stated, even though the APLIS and technology can do certain things, it is only as good and streamlined as the process and people behind it. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Friday, November 01, 2013 9:50 AM To: 'Michael Mihalik'; 'histonet' Subject: RE: [Histonet] Specimen Tracking Systems Michael, yes that is the sort of workflow item that has to be considered. We plan to use the scanned image at the gross bench to double check. Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: Michael Mihalik [mailto:mike@pathview.com] Sent: Friday, November 01, 2013 9:47 AM To: Morken, Timothy; 'Bauer, Karen L.'; 'Jesus Ellin'; 'WILLIAM DESALVO'; 'Matthew D. Roark'; 'histonet' Subject: RE: [Histonet] Specimen Tracking Systems Just a quick thought.... If you scan your requisition into the LIS after accessioning, it might not be a bad idea to have your grossing personnel review the requisition at grossing to confirm that the patient name/specimen on the paperwork matches what's in the LIS. Yes, with barcodes this shouldn't happen, but it's a nice double check. Michael Mihalik PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Friday, November 01, 2013 9:23 AM To: 'Bauer, Karen L.'; 'Jesus Ellin'; 'WILLIAM DESALVO'; 'Matthew D. Roark'; 'histonet' Subject: RE: [Histonet] Specimen Tracking Systems Karen, we are going to move to a system as you describe. At accessioning all paperwork will be scanned and none will follow the specimen. The critical trick is getting residents and pathologists to look at screens rather than hard copies. We spend way too much time printing and collating hard copies of reports ever day. Tim Morken Pathology UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: Bauer, Karen L. [mailto:Bauer.Karen@mayo.edu] Sent: Friday, November 01, 2013 9:03 AM To: Morken, Timothy; 'Jesus Ellin'; 'WILLIAM DESALVO'; 'Matthew D. Roark'; 'histonet' Subject: RE: [Histonet] Specimen Tracking Systems Importance: Low Jesus, Tim, and Bill... Wonderful communication about specimen tracking, bi-directional capabilities, and creating a histology lab that will be effective in the years to come!! I loved reading your information! We also have the Vantage system and it's helped us decrease our slide labeling error rate to 0%! It's wonderful and I'm so glad we finally had it implemented last April. Our workflow seems much more efficient, since we no longer have to perform all of those manual reconciliation steps. Yes, the bi-directionality of the systems (we have Sunquest CoPath) is a downfall of the software, but the pros of Vantage outweighed that flaw. I'm hoping that in the years to come, that will be fixed. A new question for the Histo group... We are trying to get away from printed grossing working drafts that are submitted with the slides and delivered to the pathologists. We would like the docs to scan the slide at their microscope and have the patient information show up on their computer. The pathologists still want the paper requisition from the specimen, so I suggested to have the requisition scanned and attached in the digital format. This way, when the doc scans the slide, the CoPath working draft, the patient clinical hx, and the scanned req slip can be viewed. Is anyone doing this in their lab right now? If so, I would really like to hear more about how you and your LIS made that happen. Thanks so much and Happy Friday!!!!! :) Karen Karen L. Bauer HTL/HT (ASCP) | Histology Supervisor | Pathology | MOHS Lab Supervisor | Dermatology | Phone: 715-838-3205 | bauer.karen@mayo.edu | Mayo Clinic Health System | 1221 Whipple Street | Eau Claire, WI 54702 | mayoclinichealthsystem.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Friday, November 01, 2013 10:06 AM To: 'Jesus Ellin'; 'WILLIAM DESALVO'; Matthew D. Roark; histonet Subject: RE: [Histonet] Specimen Tracking Systems Jesus, the topic of bi-directional interfaces is fraught with both technical and political issues. Technically it is certainly possible (As a software developer colleague of mine always told us - 'don't ask if I can do it, just tell me what you want. It's just software'). The issue is who pays for it, and who wants it. As I see it, the large LIS companies got caught flat-footed by the desire of histology labs to move to barcoding. Indeed, when I worked for a company that manufactured immunostainers we approached several large LIS companies about integrating our bar-code-driven instrument with their systems (we had already prototyped it in our lab with our own mockup of an LIS). I kid you not, the response of each and every one of them was "Why would you want to do that?" Next, they said they could do it if we had a customer that would pay for it. Here in Silicon Valley, where products are developed on the premise that people don't know what they want until you give it to them, that's like admitting you are clueless. Most of these LIS companies are not very proactive and are largely unimaginative in their outlook. That is why Ventana, Leica and others were able to step into that gap and develop their own systems. And at places like U of W they developed their own tracking systems and fully integrated it to their LIS because their LIS did not have anything even planned for barcoding. But now these same LIS companies have finally figured out that that the barcoding ship is leaving the dock and they are scrambling to develop their own systems to compete with the stand-alone systems like Vantage and Cerebro. Due to that competition these LIS companies may not be willing to allow true bi-directional communication - it totally undercuts anything they may develop. They are already at least 5 years behind the curve and can't allow the other companies to gain any further advantage. Tim Morken -----Original Message----- From: Jesus Ellin [mailto:JEllin@yumaregional.org] Sent: Friday, November 01, 2013 6:54 AM To: Morken, Timothy; 'WILLIAM DESALVO'; Matthew D. Roark; histonet Subject: RE: [Histonet] Specimen Tracking Systems I would like to chime in on this Bill and Tim both gave great examples of what it takes to move in this direction. But I would also like to understand why there are so many challenges in getting the data back into the APLIS with updates and orders. I would tell people the biggest concern that I have the data being produced by a system should be accessible across all gradients of the workflow and not just the unique tracking system. Not only is this tracking system positioned to help out histology with what is being explained below, but it is the foundation work in taking steps to get to the next level when talking Digital Pathology, molecular, Personalized medicine, Pathologist cockpit, and algorithm analysis. With the looming cuts coming down the pike, we have to make sure the vendors understand we cannot have barriers when it comes to interface and interoperability with our system. At the same token we cannot be charges an arm, leg and torso for interfacing what is rightfully ours and that is the patients data we produce. Bill and Tim excellent job in giving a crash course in Tracking system 101,, I do believe we should see more of this explained at professional societies meeting and also what the new environments we are going to be working in because of this technology. Just my two cents Jesus Ellin -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Thursday, October 31, 2013 3:45 PM To: 'WILLIAM DESALVO'; Matthew D. Roark; histonet Subject: RE: [Histonet] Specimen Tracking Systems I agree with Bill on this. We have Cerner Copath Plus and looked at Cerebro, Vantage and Omnitrax. Only Cerebro and Vantage were capable of working with Copath. But the problem is that Copath requires you purchase their tracking system to use as an HL-7 interface to any third party system. AND there is not two-way communication between the systems. You MUST enter orders (cases, blocks, stains) in copath and it ports orders to the other system. The only feedback is status updates to copath - you cannot add items in the other system and expect them to show up in copath. That requirement effectively doubled the price of the system. So we are using only the Copath AB&T system, which we are starting to implement now. They will finally have touch screen capability in the spring so we were satisfied with that. Touch screens were a primary spec we demanded. The key to tracking is how the tracking system works with your current LIS. Can it communicate in a true bi-directional fashion? Is it just an add on and all the tracking info is in the tracking component, not the LIS? There are tradeoffs and you need to figure out which you want to live with. We spent two years investigating all these systems, doing site visits, and going through a 6-month total LEAN evaluation. The one thing every institution told us was: LEAN your system BEFORE adding barcoding. Barcoding will NOT fix any inefficiencies you have. And you are pretty much stuck with what ever system you barcode - warts and all. So do all the hard work up front. We thought we were prepared but are daily finding all kinds of little details that have to be worked out. Detail out every single little thing about all your workflows before starting anything. Streamline everything. Cassette printing is the heart of barcoding. The barcoded (actually usually 2D matrix codes) on the cassette drive grossing, tracking through processing, embedding and cutting. If that does not work reliably your system will not work. We went with Leica cassette printers. They are huge, but fast (5 sec per cass). We were going to use thermo, but they had their printhead problem and we dropped the order. They are 20+ seconds per cassttes, tho have a very compact footprint. You will find there are tradeoffs to everything. Slide labeling is the other half of the equation. They must be reliable and survive all histology procedures. We will use thermal transfer label printers rather than direct slide (too expensive- we will have a printer at each microtome- and cumbersome to change out slide types). Slide labeling is a HUGE deal - you have to be sure it works for everything and can be used in your immuno stainers and coverslipping machines. We are using either Brady StainerBondz labels or LabTags Xylituff labels. These were the only ones that had the size we wanted (23 x 19 mm) and survived all processes. General data StainerShield are also good, but only come in 22 x 22 size. Plus are the most expensive. You will find it much easier if you use labels and ribbons from one manufacturer, designed to use with specific printers. That simplifies a lot of the effort. We have tried to mix and match but it is very difficult because all manufacturers do not make matching labels and ribbons for all printers. Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO Sent: Thursday, October 31, 2013 3:01 PM To: Matthew D. Roark; histonet Subject: RE: [Histonet] Specimen Tracking Systems My lab chose the Ventana Vantage System w/ Thermo PrintMate cassette printers. We are a multi-location system and Vantage works well for us. We wanted a robust tracking and flexible quality assurance system and Ventana provided what we needed and wanted. I suggest you look at the big four to start your evaluation: Ventana - Vantage Leica- Cerebro Thermo- OmniTrax General Data - HTS The larger companies have more interfaces built for the different LIS used in Histology. There are many smaller companies that offer tracking systems, but make sure they can connect w/ your LIS. After product selection, support is critical. Sunquest and Cerner Millennium now offer tracking and quality assurance modules. And I imagine there are other tracking systems built into more LIS. You will also want to evaluate multiple printers for your cassettes. Always keep in mind the amount of information you will be adding to the cassette a this will reduce the number of cassettes/ minute. Start w/ the tracking system vendor and see what the have validated. Consider the upkeep and maintenance of the printer. We choose heat transfer, but there are Laser and Ink-jet that work really well. We chose to point of generation and wanted a very small footprint to place the printers in the grossing stations. We also choose not to use a slide printing system and decided the bar coded labels at microtomy was the best solution for us. We wanted less maintenance and steady throughput. Easier to load a roll of labels than keeping a printer up and running. Last, but certainly not least, support. You will need a ton of support to work through this project: interfaces, IT upgrades, software adjustments, WORKFLOW changes and training. Plan and re-plan before you make your selection. And negotiate hard, the pricing is all over the place for the systems. William DeSalvo, BS HTL(ASCP) Production Manager-Anatomic Pathology Chair, NSH Quality Management Committee Board Member, Digital Pathology Association Owner/Consultant, Collaborative Advantage Consulting > From: mroark@sfmc.net > To: histonet@lists.utsouthwestern.edu > Date: Thu, 31 Oct 2013 20:21:47 +0000 > Subject: [Histonet] Specimen Tracking Systems > > What systems are people using for specimen tracking? I'm looking for > the whole package of cassette printers, work station slide printers, tracking software, etc... > > Any help and recommendations would be appreciated! > > > > Matthew Roark- HT/HTL(ASCP)CM > Histology Specialist > Saint Francis Medical Center > 211 Saint Francis Drive > Cape Girardeau, MO 63703 > 573-331-3982 > mroark@sfmc.net > http://www.sfmc.net > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ From brett_connolly <@t> merck.com Fri Nov 1 12:50:53 2013 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Fri Nov 1 12:50:58 2013 Subject: [Histonet] Jak 3 antibody Message-ID: HI all, Anyone have a recommendation for a Jak 3 antibody for FFPE tissue. We tried a couple from Santa Cruz without any success. Thanks, Brett Brett M. Connolly, Ph.D. Principal Scientist, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From Shelly.Cooke <@t> centrahealth.com Fri Nov 1 13:32:24 2013 From: Shelly.Cooke <@t> centrahealth.com (Shelly.Cooke@centrahealth.com) Date: Fri Nov 1 13:32:27 2013 Subject: [Histonet] Ventana vs Leica IHC Message-ID:
We've never used a Leica IHC stainer in our lab so I can't give a comparison of the two but we have had 3 Ventana instruments in our lab over the years. We have the Ultra Benchmark now and it is a big upgrade from the XT because of the individual test drawers. Our machine has Ultimate Reagent Access so we can start runs early and add reagents and slides throughout the day as orders come in. We don't have to wait and batch our runs and possibly run out of space on the machine like we did with the XT. The Ventana Ultra has improved workflow in our lab and the support has been exceptional in our area.
 
Shelly Cooke
Histology Supervisor
Pathology Consultants
1914 Thomson Drive
Lynchburg, VA 24501





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 From tbraud <@t> holyredeemer.com Fri Nov 1 14:05:10 2013 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Fri Nov 1 14:05:10 2013 Subject: [Histonet] Billing question Message-ID: I have a billing question that I would sure like to hear what others are doing, please. In AP billing, when billing for CPT #88321 "Consultation and report on referred slides prepared elsewhere" do you bill both a technical and professional component, or professional only? Any input would be appreciated. Sincerely, Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. From Joyce.Weems <@t> emoryhealthcare.org Fri Nov 1 14:13:27 2013 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Fri Nov 1 14:13:35 2013 Subject: [Histonet] RE: Billing question In-Reply-To: References: Message-ID: Professional only.. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Terri Braud Sent: Friday, November 01, 2013 3:05 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Billing question I have a billing question that I would sure like to hear what others are doing, please. In AP billing, when billing for CPT #88321 "Consultation and report on referred slides prepared elsewhere" do you bill both a technical and professional component, or professional only? Any input would be appreciated. Sincerely, Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From rheyna <@t> lumc.edu Fri Nov 1 18:04:10 2013 From: rheyna <@t> lumc.edu (Roger Heyna) Date: Fri Nov 1 18:04:26 2013 Subject: [Histonet] Lead Tech Job Description Message-ID: <5273ED1A020000230008651F@gwgwia1.luhs.org> Hi Everyone, I hope you all had a fun Halloween. I am looking for a few examples of job descriptions for lead (or senior) technicians in histology. I'm curious about what responsibilities you assign to your lead histotechs. Examples of tasks would be staff scheduling, supply ordering, daily task assignments, etc. If you send a copy of the actual job description, it would help me construct one on my end. Thanks in advance for your help! Roger Maywood, IL From pev <@t> xs4all.nl Sat Nov 2 11:10:41 2013 From: pev <@t> xs4all.nl (P.E. Visser) Date: Sat Nov 2 11:10:51 2013 Subject: [Histonet] request for validation form example. Message-ID: <000b01ced7e6$14c24910$3e46db30$@xs4all.nl> dear frends, probably I am number 1000000000 who asks this question. but I searched the internet for hours and could not find any sample. we are starting to create a QA procedure for validating our special stains and the improvements we make in the recepies. who is willing to send me a word file or pdf that I can use as a sampel? many thanks in advance Piet pathology Bronovo The Hage ( The Netherlands) From Richard.Cartun <@t> hhchealth.org Sun Nov 3 14:49:44 2013 From: Richard.Cartun <@t> hhchealth.org (Cartun, Richard) Date: Sun Nov 3 14:49:52 2013 Subject: [Histonet] RE: cell block staining issues In-Reply-To: References: <52720D58.8010301@upmc.fr>, Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E01893432@HHCEXCHMB05.hhcsystem.org> I always recommend that cytology specimens be collected (or rinsed) in sterile saline or RPMI. Although fixation in alcohol can preserve some proteins very nicely, it can also damage others. CytoLyt contains methanol. Interesting that your Cytopath MDs still use B72.3; most labs have stopped using it. I have to admit that I still offer it, but we don't do it that often. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax richard.cartun@hhchealth.org ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Martha Ward-Pathology [mward@wakehealth.edu] Sent: Thursday, October 31, 2013 8:18 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cell block staining issues We have something of a mystery here and I am hoping someone can help. My cytopath docs are saying that our B72.3 (TAG-72) antibody is not working well in cell blocks created from fluids (plural), etc. and that this is a fairly recent development (over the past 5-6 weeks or so). The surgical cases we have stained look great as does our control, which is placed on each slide. We have not changed lot numbers of antibody or made any up recently and the antibody is not expired, or even close. The only thing we can find that is different is that cytology has been rinsing the needles out with a product called Cytolyt, instead of saline and they started this around the first of August. Does anyone know if this product could cause any interference with our staining? The docs just say that the tumor cells are not staining and we are sort of at a loss here. Thanks in advance for your help. Martha Ward, MT (ASCP) QIHC Manager [Wake Forest Baptist Health] Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mward@wakehealth.edu This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From lpwenk <@t> sbcglobal.net Mon Nov 4 07:22:15 2013 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Mon Nov 4 07:22:22 2013 Subject: [Histonet] 2014 NSH Teleconferences/Webinars Message-ID: <65529D60E818484C979B2AB93EA7C719@HP2010> Promotion about NSH teleconferences for next year ? if not interested, delete now. FYI ? the 2014 NSH webinar schedule is now available, and so is signing up. http://www.nsh.org/content/2014-nsh-laboratory-webinar-series-registration-now-open If this link doesn?t work, go to NSH webpage www.nsh.org Part way down on the left it says 2014 NSH Laboratory Webinar Series Registration Now Open Just click on that. One each month, usually 4th Wed of month (unless NSH Symposium or a major holiday interferes), from 1-2 pm Eastern Time. Can have as many people attend as you want, and each gets 1 hour CE. Your lab also gets a link afterwards, and for those who couldn?t attend (working, off-shift, vacation, etc.), they can participate up to 2 years later, and still get CE. $125 for each month, $1350 for all 12 months if you sign up by 1/22/14 (savings of $150, or better than buy 11, get 1 free). IHC, histology, autopsy, safety ergonomics. Peggy A. Wenk, HTL(ASCP)SLS NSH Webinar Coordinator (No, I don?t get any money for doing this job or advertising it ? I?m a volunteer) From egray <@t> hsc.wvu.edu Mon Nov 4 07:35:21 2013 From: egray <@t> hsc.wvu.edu (Gray, Ed) Date: Mon Nov 4 07:35:37 2013 Subject: [Histonet] Billing question Message-ID: <113b961853594d70a058b7da8b6bbe68@BN1PR05MB406.namprd05.prod.outlook.com> Professional only Ed Gray | Pathology IT Analyst | Phone: 304-293-2945 | Fax 304-293-1627 | WVU Healthcare l egray@wvuhealthcare.com Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Terri Braud Sent: Friday, November 01, 2013 3:05 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Billing question I have a billing question that I would sure like to hear what others are doing, please. In AP billing, when billing for CPT #88321 "Consultation and report on referred slides prepared elsewhere" do you bill both a technical and professional component, or professional only? Any input would be appreciated. Sincerely, Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. From ree3 <@t> leicester.ac.uk Mon Nov 4 08:39:24 2013 From: ree3 <@t> leicester.ac.uk (Edwards, Richard E.) Date: Mon Nov 4 08:39:52 2013 Subject: [Histonet] basement membranes Message-ID: <7722595275A4DD4FA225B92CDBF174A101B0DAD714EB@EXC-MBX3.cfs.le.ac.uk> Best technique, tinctorial or otherwise of identifying, with a view to measuring their width, many thanks. Richard Edwards Leicester University U.K. From ahc53 <@t> georgetown.edu Mon Nov 4 09:20:52 2013 From: ahc53 <@t> georgetown.edu (Anna Coffey) Date: Mon Nov 4 09:20:57 2013 Subject: [Histonet] Problems with H&E Bleeding Message-ID: Hello Histonetters, We have recently noticed some bleeding of our H&E stained tissues and I was curious to hear if anyone has had problems with this before. There will just be a faint halo of stain around the tissue. This seems to be happening inconsistently (various tissues, even differences within the same stained batch) and has now occurred with both paraffin-embedded and frozen tissue, so with 2 different programs in our autostainer. If you've seen this in your lab before, were you able to isolate the cause and fix it? I'd be very appreciative of any advice you may have about what we can do to fix this problem. Thanks, Anna -- Anna Coffey Senior Histology Technician Department of Oncology Histopathology and Tissue Shared Resource LR-10 Pre-Clinical Sciences Building Lombardi Comprehensive Cancer Center Georgetown University 202-687-7890 ahc53@georgetown.edu From Vickroy.Jim <@t> mhsil.com Mon Nov 4 09:38:04 2013 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Mon Nov 4 09:38:08 2013 Subject: [Histonet] Aspergillus tissue blocks for controls Message-ID: We are in desperate needs of obtaining GMS controls. In the old days we had all the fungal specimens we needed. Today it is getting hard to find these tissues or controls. Does anyone have any idea where we can get tissue blocks with Aspergillus or does anyone have any other suggestions about GMS controls. James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From lcolbert <@t> pathmdlabs.com Mon Nov 4 09:42:50 2013 From: lcolbert <@t> pathmdlabs.com (Laurie Colbert) Date: Mon Nov 4 09:47:08 2013 Subject: [Histonet] Problems with H&E Bleeding In-Reply-To: References: Message-ID: <12ECD7346266D74691EC2BFC75285E452F362E77@BFL323E10.pathmdlabs.local> You probably have water and/or alcohol contamination in your xylenes. Make sure your dehydrating alcohols after the eosin are really clean. Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anna Coffey Sent: Monday, November 04, 2013 7:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Problems with H&E Bleeding Hello Histonetters, We have recently noticed some bleeding of our H&E stained tissues and I was curious to hear if anyone has had problems with this before. There will just be a faint halo of stain around the tissue. This seems to be happening inconsistently (various tissues, even differences within the same stained batch) and has now occurred with both paraffin-embedded and frozen tissue, so with 2 different programs in our autostainer. If you've seen this in your lab before, were you able to isolate the cause and fix it? I'd be very appreciative of any advice you may have about what we can do to fix this problem. Thanks, Anna -- Anna Coffey Senior Histology Technician Department of Oncology Histopathology and Tissue Shared Resource LR-10 Pre-Clinical Sciences Building Lombardi Comprehensive Cancer Center Georgetown University 202-687-7890 ahc53@georgetown.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpwenk <@t> sbcglobal.net Mon Nov 4 12:38:25 2013 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Mon Nov 4 12:38:30 2013 Subject: [Histonet] basement membranes In-Reply-To: <7722595275A4DD4FA225B92CDBF174A101B0DAD714EB@EXC-MBX3.cfs.le.ac.uk> References: <7722595275A4DD4FA225B92CDBF174A101B0DAD714EB@EXC-MBX3.cfs.le.ac.uk> Message-ID: <3BC14F9010C4499A88E5351B42787F0B@HP2010> Electron Microscope. Even if you did a periodic acid-methenamine silver stain (PASM, Jones), which in my opinion is the best histology stain, since it is a silver stain, you can get different thicknesses of basement membrane (bm) by leaving it in longer or shorter than is optimal for that patient's bm thickness. Peggy A. Wenk, HTL(ASCP)SLS -----Original Message----- From: Edwards, Richard E. Sent: Monday, November 04, 2013 9:39 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] basement membranes Best technique, tinctorial or otherwise of identifying, with a view to measuring their width, many thanks. Richard Edwards Leicester University U.K. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CDavis <@t> che-east.org Mon Nov 4 12:45:26 2013 From: CDavis <@t> che-east.org (Davis, Cassie) Date: Mon Nov 4 12:45:27 2013 Subject: [Histonet] (no subject) Message-ID: <08861B9CF6C7774E874635A4818AE37B01C9AC44F5@CHEXCMS01.one.ads.che.org> Jim, We get our Fungus, GMS Aspergillus controls (slides) and many of our hard to find control slides from Newcomer Supply 1-800-383-7799 or 608-831-7888 Cassandra Davis CDavis@che-east.org 302-575-8095 Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From CDavis <@t> che-east.org Mon Nov 4 12:46:23 2013 From: CDavis <@t> che-east.org (Davis, Cassie) Date: Mon Nov 4 12:47:14 2013 Subject: [Histonet] GMS fungus controls In-Reply-To: <08861B9CF6C7774E874635A4818AE37B01C9AC44F5@CHEXCMS01.one.ads.che.org> References: <08861B9CF6C7774E874635A4818AE37B01C9AC44F5@CHEXCMS01.one.ads.che.org> Message-ID: <08861B9CF6C7774E874635A4818AE37B01C9AC44F9@CHEXCMS01.one.ads.che.org> Jim, We get our Fungus, GMS Aspergillus controls (slides) and many of our hard to find control slides from Newcomer Supply 1-800-383-7799 or 608-831-7888 Cassandra Davis CDavis@che-east.org 302-575-8095 Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From foreightl <@t> gmail.com Mon Nov 4 14:07:06 2013 From: foreightl <@t> gmail.com (Patrick Laurie) Date: Mon Nov 4 14:07:12 2013 Subject: [Histonet] Aspergillus tissue blocks for controls In-Reply-To: References: Message-ID: Hi Jim, I have found a couple of ways. First, if you are fortunate to have a micro department nearby, they can make a very nice one for you (a Histotip in Sakura's Histologic). Another method, which doesn't work the best, can be moldy orange peel. And the easiest, you can create your own (moldy sausage) or even take some human tissue (fresh lung works the best) and leave it with some moldy meat, then you can get a great one. Good luck! On Mon, Nov 4, 2013 at 10:38 AM, Vickroy, Jim wrote: > We are in desperate needs of obtaining GMS controls. In the old days we > had all the fungal specimens we needed. Today it is getting hard to find > these tissues or controls. Does anyone have any idea where we can get > tissue blocks with Aspergillus or does anyone have any other suggestions > about GMS controls. > > James Vickroy BS, HT(ASCP) > > Surgical and Autopsy Pathology Technical Supervisor > Memorial Medical Center > 217-788-4046 > > > ________________________________ > This message (including any attachments) contains confidential information > intended for a specific individual and purpose, and is protected by law. If > you are not the intended recipient, you should delete this message. Any > disclosure, copying, or distribution of this message, or the taking of any > action based on it, is strictly prohibited. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie(HT)ASCP QIHC Histology Manager Celligent Diagnostics, LLC 101 East W.T. Harris Blvd | Suite 1212 | Charlotte, NC 28262 Work: 704-970-3300 Cell: 704-266-0869 From pev <@t> xs4all.nl Mon Nov 4 14:28:33 2013 From: pev <@t> xs4all.nl (P.E. Visser) Date: Mon Nov 4 14:28:36 2013 Subject: [Histonet] Problems with H&E Bleeding Message-ID: <002301ced99c$7098c9f0$51ca5dd0$@xs4all.nl> Dear anna I had while working for Baker chemicals, a customer in Germany who had a similar problem there H&E turned purple. the red disappeared. the environment around the section contained little red droplets (only visible under high magnification). after some time (because at first they clamed noting was changed) it became clear that they skipped ethanol 100 % and just used the cheaper 96% instead. when evaluated they tested 3 slides and that worked. but after 300 - 500 slides the xylene was saturated with water. eosin being soluble in water migrates from the section to the water emulate in the xylene. so I would test the ethanol you use. 3 times 100% is in my view a must. I also on time in Scotland was confronted with xylene that was sold as non recycled, but in fact it was. and sadly the supplier used an open container to speed up cooling down. the rain was the source of the water. it lead to several problems and took a long time before the we could find the source of these problems. wish you success with solving yours. regards piet From Bruce_Palmatier <@t> vwr.com Mon Nov 4 15:00:59 2013 From: Bruce_Palmatier <@t> vwr.com (Bruce_Palmatier@vwr.com) Date: Mon Nov 4 15:01:29 2013 Subject: [Histonet] AUTO: Bruce Palmatier is out of the office (returning 11/08/2013) Message-ID: I am out of the office until 11/08/2013. I will be out of the office from Nov 4- Nov 8. If you have a question about an order or need assistance placing an order, or if the matter is urgent, please call 877-881-1192 between 8am and 8pm and a VWR Healthcare Service associate will assist you. For other matters, I will respond to emails within 24 hours. If you require pricing information, quotes, product information, or other assistance, Tim Rafferty is the new VWR Healthcare Sales Rep who's taken my place. He can be reached at timothy_rafferty@vwr.com or (717) 668-9045. Thank You, Bruce Palmatier Market Portfolio Manager VWR Healthcare bruce_palmatier@vwr.com mobile: 484.319.5563 fax: 484-881-7307 Customer Service: 877.881.1192 Fax: 484.881.6486 Customer Service email: HEALTHCARESERVICE@VWR.COM Note: This is an automated response to your message "Histonet Digest, Vol 120, Issue 5" sent on 11/4/2013 1:06:11 PM. This is the only notification you will receive while this person is away. From hans <@t> histologistics.com Mon Nov 4 15:46:37 2013 From: hans <@t> histologistics.com (Hans B Snyder) Date: Mon Nov 4 15:46:42 2013 Subject: [Histonet] Aspergillus tissue blocks for controls In-Reply-To: References: Message-ID: We have tried the moldy peel also but the pathologists did not like this and would not accept any controls of this form. I don't blame them they want to see it naturally occurring. Hans B Snyder Histologistics 60 Prescott Street Worcester, MA 01605 508-308-7800 hans@histologistics.com On Mon, Nov 4, 2013 at 3:07 PM, Patrick Laurie wrote: > Hi Jim, > > I have found a couple of ways. First, if you are fortunate to have a micro > department nearby, they can make a very nice one for you (a Histotip in > Sakura's Histologic). Another method, which doesn't work the best, can be > moldy orange peel. And the easiest, you can create your own (moldy > sausage) or even take some human tissue (fresh lung works the best) and > leave it with some moldy meat, then you can get a great one. > > Good luck! > > > On Mon, Nov 4, 2013 at 10:38 AM, Vickroy, Jim > wrote: > > > We are in desperate needs of obtaining GMS controls. In the old days we > > had all the fungal specimens we needed. Today it is getting hard to find > > these tissues or controls. Does anyone have any idea where we can get > > tissue blocks with Aspergillus or does anyone have any other suggestions > > about GMS controls. > > > > James Vickroy BS, HT(ASCP) > > > > Surgical and Autopsy Pathology Technical Supervisor > > Memorial Medical Center > > 217-788-4046 > > > > > > ________________________________ > > This message (including any attachments) contains confidential > information > > intended for a specific individual and purpose, and is protected by law. > If > > you are not the intended recipient, you should delete this message. Any > > disclosure, copying, or distribution of this message, or the taking of > any > > action based on it, is strictly prohibited. > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > -- > > Patrick Laurie(HT)ASCP QIHC > > Histology Manager > > Celligent Diagnostics, LLC > > 101 East W.T. Harris Blvd | Suite 1212 | Charlotte, NC 28262 > > Work: 704-970-3300 Cell: 704-266-0869 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From dmccaig <@t> ckha.on.ca Mon Nov 4 16:31:18 2013 From: dmccaig <@t> ckha.on.ca (Diana McCaig) Date: Mon Nov 4 16:31:30 2013 Subject: [Histonet] Aspergillus tissue blocks for controls In-Reply-To: References: Message-ID: As well we have used mushroom and other moldy food products but it only demonstrates the mold and not tissue elements. But you can mince tissue and mix it with the fungus or mold so it one big segment and this gave more desirable results. Diana -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hans B Snyder Sent: November-04-13 4:47 PM To: Patrick Laurie Cc: histonet@lists.utsouthwestern.edu; Vickroy, Jim Subject: Re: [Histonet] Aspergillus tissue blocks for controls We have tried the moldy peel also but the pathologists did not like this and would not accept any controls of this form. I don't blame them they want to see it naturally occurring. Hans B Snyder Histologistics 60 Prescott Street Worcester, MA 01605 508-308-7800 hans@histologistics.com On Mon, Nov 4, 2013 at 3:07 PM, Patrick Laurie wrote: > Hi Jim, > > I have found a couple of ways. First, if you are fortunate to have a > micro department nearby, they can make a very nice one for you (a > Histotip in Sakura's Histologic). Another method, which doesn't work > the best, can be moldy orange peel. And the easiest, you can create > your own (moldy > sausage) or even take some human tissue (fresh lung works the best) > and leave it with some moldy meat, then you can get a great one. > > Good luck! > > > On Mon, Nov 4, 2013 at 10:38 AM, Vickroy, Jim > wrote: > > > We are in desperate needs of obtaining GMS controls. In the old days we > > had all the fungal specimens we needed. Today it is getting hard to find > > these tissues or controls. Does anyone have any idea where we can get > > tissue blocks with Aspergillus or does anyone have any other > > suggestions about GMS controls. > > > > James Vickroy BS, HT(ASCP) > > > > Surgical and Autopsy Pathology Technical Supervisor Memorial > > Medical Center > > 217-788-4046 > > > > > > ________________________________ > > This message (including any attachments) contains confidential > information > > intended for a specific individual and purpose, and is protected by law. > If > > you are not the intended recipient, you should delete this message. > > Any disclosure, copying, or distribution of this message, or the > > taking of > any > > action based on it, is strictly prohibited. > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > -- > > Patrick Laurie(HT)ASCP QIHC > > Histology Manager > > Celligent Diagnostics, LLC > > 101 East W.T. Harris Blvd | Suite 1212 | Charlotte, NC 28262 > > Work: 704-970-3300 Cell: 704-266-0869 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Valerie.Hannen <@t> parrishmed.com Tue Nov 5 04:47:53 2013 From: Valerie.Hannen <@t> parrishmed.com (Hannen, Valerie) Date: Tue Nov 5 05:04:10 2013 Subject: [Histonet] FDA Disclaimer Message-ID: <450B7A81EDA0C54E97C53D60F00776C3232B4BB696@isexstore03> Good Morning... I have a question about the FDA disclaimer for Immuno's. If we are not doing the staining of Immuno's in our lab, but our Pathologists are interpreting those that are stained at our reference lab, are we still required to put the FDA disclaimer on our Path reports for those antibodies that require the disclaimer? Thanks so much!! Valerie Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.hannen@parrishmed.com =================== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" =================== From Allison.Scott <@t> harrishealth.org Tue Nov 5 08:50:15 2013 From: Allison.Scott <@t> harrishealth.org (Scott, Allison D) Date: Tue Nov 5 08:50:22 2013 Subject: [Histonet] FW: The results of your email commands In-Reply-To: References: Message-ID: -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Sent: Tuesday, November 05, 2013 8:48 AM To: Scott, Allison D Subject: The results of your email commands The results of your email command are provided below. Attached is your original message. - Results: Ignoring non-text/plain MIME parts - Unprocessed: Sent: Tuesday, November 05, 2013 8:47 AM To: histonet-request@lists.utsouthwestern.edu Subject: FW: FaxitronX-Ray digital imaging From: Scott, Allison D Sent: Tuesday, November 05, 2013 8:46 AM To: histonet-request@lists.utsouthwestern.edu Subject: Faxitron/X-Ray digital imaging Hello to all in histoland. Our chief pathologist is interested in getting = a faxitron xray digital imaging machine. Is anyone out there using this te= chnology and can you suggest a good company in which to order one from. An= y help in this will be greatly appreciated. Allison Scott HT(ASCP) Supervisor, Histology Lab LBJ Hospital Harris Health System - Ignored: Office: 713-566-2148 Lab: 713-566-5287 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from = your computer system. To the extent the information in this e-mail and any attachments contain = protected health information as defined by the Health Insurance Portability = and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and = 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/o= r = privileged. This e-mail may also be confidential and/or privileged under = Texas law. The e-mail is for the use of only the individual or entity name= d = above. If you are not the intended recipient, or any authorized = representative of the intended recipient, you are hereby notified that any = review, dissemination or copying of this e-mail and its attachments is = strictly prohibited. - Done. CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. From relia1 <@t> earthlink.net Tue Nov 5 09:08:46 2013 From: relia1 <@t> earthlink.net (Pam Barker) Date: Tue Nov 5 09:09:02 2013 Subject: [Histonet] Hot Histology Career Opportunities Alert from Pam Barker at RELIA Solutions!! Even if you are happy where you are take a look please we offer a 500.00 referral fee and it's almost time for Christmas shopping!!! 11/5/2013 Message-ID: <00dc01ceda38$ecf3afa0$c6db0ee0$@earthlink.net> Hi Histonetters!! I hope you are off to the start of a great week on this beautiful Fall Day. I wanted to drop you a line about some of the opportunities that I am currently working on. These clients aren't just "kicking tires" they are ready to interview and ready to hire. If you are looking to make a change now or make a commitment now and the actual change after the holidays if you are the right person for my client they will do what it takes to get you I guarantee it!! So grab a cup of coffee or a mug of tea and something pumpkin flavored (isn't everything pumpkin flavored this time of year?) and please take a minute to peruse my list of current openings. All of the positions I work with are fulltime permanent positions with some of the best facilities Nationwide. My clients offer excellent compensation including competitive salaries, great benefits, relocation assistance/sign on bonuses. Here is a list of my current openings: HISTOLOGY SUPERVISORS/MANAGERS: WA OH MA GA Pathology Supervisor - Seattle, WA Lead Pathology Tech - East of Columbus, OH Histology Supervisor - Boston, MA Histology Supervisor - Atlanta, GA HISTOTECHNICIANS/HISTOTECHNOLOGISTS: VA OH NM MA Histology Tech - Roanoke, VA Histotechnician Day shift -East of Columbus, OH Grossing Histotechnician Dayshift - Las Cruces, NM Histotechnician - Nights - Nashville, TN Night Shift Grossing Histotech - Boston, MA If you or any of your friends would like more information on any of these positions or if you would like to discuss opportunities in other areas or future job searches please contact me. I would be more than happy to assist you. You can reach me at 866-607-3542 or relia1@earthlink.net Remember I offer over 20 years of recruiting and employment counseling experience, knowledgeable, confidential and responsive service to you and your friends and a permanent placement practice dedicated to the histology profession. I work with facilities nationwide and I will keep your resume confidential. I will only represent you to jobs you tell me you are interested in looking into. Remember. It never hurts to keep an eye open even if you are happy in your present job. Pam - 866-607-3542 (866-60RELIA) Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From ryeo <@t> foxmail.wchosp.org Tue Nov 5 09:17:26 2013 From: ryeo <@t> foxmail.wchosp.org (Richard Yeo) Date: Tue Nov 5 09:17:42 2013 Subject: [Histonet] (no subject) Message-ID: <17E893BEFAF44C0EAD05AF8876EDAF80.MAI@foxmail.wchosp.org> I have an abundance of fungus controls. I need afb and h.pylori. I would be me know an Thanks Richard Yeo ryeo@foxma From Allison.Scott <@t> harrishealth.org Tue Nov 5 10:00:11 2013 From: Allison.Scott <@t> harrishealth.org (Scott, Allison D) Date: Tue Nov 5 10:00:15 2013 Subject: [Histonet] Faxitron Xray digital imaging Message-ID: Hello to all in histoland. Our chief pathologist is interested in getting a faxitron xray digital imaging machine. Is anyone out there using this technology and can you suggest a company in which to order one from. Any help in this will be greatly appreciated. Allison Scott HT(ASCP) Supervisor, Histology Lab LBJ Hospital Harris Health System Office: 713-566-2148 Lab: 713-566-5287 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. From wdesalvo.cac <@t> outlook.com Tue Nov 5 10:47:16 2013 From: wdesalvo.cac <@t> outlook.com (WILLIAM DESALVO) Date: Tue Nov 5 10:47:22 2013 Subject: [Histonet] Faxitron Xray digital imaging In-Reply-To: References: Message-ID: We have used the Faxitron instrument for the past 3 years at multiple sites. Faxitron creates a digital image of a specimen quickly and safely and a t multiple magnifications. The instrument is placed in either Surgery or Surgical Pathology (SP) and we use it primarily on breast cases to assist in orientation and gross dissection, identifying radioactive seeds or locating micro calcification. The instrument is easy to use, safe and you can connect to multiple LIS systems and a Hospital PACS. There is no code to charge for use when used in SP, only when used by Radiology or Surgery. There is only one company that manufactures and sells the instrument: Faxitron Bioptics, LLC Tucson, AZ 877-910-0030 The are willing to bring a unit to your site for demonstration and you may be able to talk them into leaving for a evaluation. William DeSalvo, BS HTL(ASCP) > From: Allison.Scott@harrishealth.org > To: histonet@lists.utsouthwestern.edu > Date: Tue, 5 Nov 2013 16:00:11 +0000 > Subject: [Histonet] Faxitron Xray digital imaging > > Hello to all in histoland. Our chief pathologist is interested in getting a faxitron xray digital imaging machine. Is anyone out there using this technology and can you suggest a company in which to order one from. Any help in this will be greatly appreciated. > > > > > Allison Scott HT(ASCP) > Supervisor, Histology Lab > LBJ Hospital > Harris Health System > Office: 713-566-2148 > Lab: 713-566-5287 > > > CONFIDENTIALITY NOTICE: > If you have received this e-mail in error, please immediately notify the > sender by return e-mail and delete this e-mail and any attachments from > your computer system. > > To the extent the information in this e-mail and any attachments contain > protected health information as defined by the Health Insurance Portability > and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and > 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or > privileged. This e-mail may also be confidential and/or privileged under > Texas law. The e-mail is for the use of only the individual or entity named > above. If you are not the intended recipient, or any authorized > representative of the intended recipient, you are hereby notified that any > review, dissemination or copying of this e-mail and its attachments is > strictly prohibited. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From thigginsht <@t> msn.com Tue Nov 5 13:03:54 2013 From: thigginsht <@t> msn.com (Tim Higgins) Date: Tue Nov 5 13:03:58 2013 Subject: [Histonet] FW: Fungus Controls In-Reply-To: References: Message-ID: I have used orange peel for years. I never had a problem with the pathologist not approving the control for use on patient stains. Its "naturally occurring", a petri dish would not be naturally occurring in my mind. Once I verified the orange peel does stain the fungus the same way it does in human tissue by doing a side by side staining the pathologist were fine with it. Actually happy I was able to save some money. Most controls you get from other source are not human either, usually it some type of animal. Try the orange peel. I did like the idea of using the some type of moldy meat or having your micro department do something for you!!!! I have never tried that, but if it works that would be awesome. Thanks, Timothy N. Higgins, HT (ASCP), QIHC > Message: 4 > Date: Mon, 4 Nov 2013 15:07:06 -0500 > From: Patrick Laurie > Subject: Re: [Histonet] Aspergillus tissue blocks for controls > To: "Vickroy, Jim" > Cc: "histonet@lists.utsouthwestern.edu" > > Message-ID: > > Content-Type: text/plain; charset=ISO-8859-1 > > Hi Jim, > > I have found a couple of ways. First, if you are fortunate to have a micro > department nearby, they can make a very nice one for you (a Histotip in > Sakura's Histologic). Another method, which doesn't work the best, can be > moldy orange peel. And the easiest, you can create your own (moldy > sausage) or even take some human tissue (fresh lung works the best) and > leave it with some moldy meat, then you can get a great one. > > Good luck! > > > On Mon, Nov 4, 2013 at 10:38 AM, Vickroy, Jim wrote: > > > We are in desperate needs of obtaining GMS controls. In the old days we > > had all the fungal specimens we needed. Today it is getting hard to find > > these tissues or controls. Does anyone have any idea where we can get > > tissue blocks with Aspergillus or does anyone have any other suggestions > > about GMS controls. > > > > James Vickroy BS, HT(ASCP) > > > > Surgical and Autopsy Pathology Technical Supervisor > > Memorial Medical Center > > 217-788-4046 > > > > From egray <@t> hsc.wvu.edu Tue Nov 5 13:20:42 2013 From: egray <@t> hsc.wvu.edu (Gray, Ed) Date: Tue Nov 5 13:22:45 2013 Subject: [Histonet] RE: Histonet Digest, Vol 120, Issue 6 In-Reply-To: References: Message-ID: <6a889df9e216449bbac73ab5d795aa71@BN1PR05MB406.namprd05.prod.outlook.com> You actually need the performing lab's disclaimer. We've sent stains and other procedures to several other labs. We refer to the disclaimers as ASR's (analyte specific reagents) and build templates for each lab's specific comments in our APLIS. Ed Gray | Pathology IT Analyst | Phone: 304-293-2945 | Fax 304-293-1627 | WVU Healthcare l egray@wvuhealthcare.com Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. Message: 9 Date: Tue, 5 Nov 2013 05:47:53 -0500 From: "Hannen, Valerie" Subject: [Histonet] FDA Disclaimer To: "Histonet Post (histonet@lists.utsouthwestern.edu)" Message-ID: <450B7A81EDA0C54E97C53D60F00776C3232B4BB696@isexstore03> Content-Type: text/plain; charset="us-ascii" Good Morning... I have a question about the FDA disclaimer for Immuno's. If we are not doing the staining of Immuno's in our lab, but our Pathologists are interpreting those that are stained at our reference lab, are we still required to put the FDA disclaimer on our Path reports for those antibodies that require the disclaimer? Thanks so much!! Valerie Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.hannen@parrishmed.com =================== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" =================== From jwood <@t> fairchildmed.org Tue Nov 5 15:41:14 2013 From: jwood <@t> fairchildmed.org (Jean Wood) Date: Tue Nov 5 15:41:22 2013 Subject: [Histonet] RE: FDA Disclaimer (Hannen, Valerie) In-Reply-To: References: Message-ID: <87A4EF3F1FBA9D42A7D1EC82F031C0B80A4FD115D6@cliff> Valerie, If your pathologist is reading your IHC send-outs then he is most likely contracted through that third party company (he gets PC compensation) and they do the technicial work. In our case, our pathologist signs out our routine H&E Final Surgical Pathology Report and when he reads the IHC he uses their report portal to issue those results using their letterhead with the FDA disclaimer attached at the end of the report. Sometimes he may incorporate the IHC results in the original report as revised but we always piggy back the third party IHC report (with digital pathology images) to the original. It might be best to contact the IHC company representative and find out excatly who is legally responsible for the disclaimer - the technical service provider or the proffesional. Jean Wood BS, HT Fairchild Medical Center Pathology Dept. Ph:530.841.6243 Fax:530.841.6232 jwood@fairchildmed.org ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu [histonet-request@lists.utsouthwestern.edu] Sent: Tuesday, November 05, 2013 10:05 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 120, Issue 6 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: basement membranes (Lee & Peggy Wenk) 2. (no subject) (Davis, Cassie) 3. GMS fungus controls (Davis, Cassie) 4. Re: Aspergillus tissue blocks for controls (Patrick Laurie) 5. Problems with H&E Bleeding (P.E. Visser) 6. AUTO: Bruce Palmatier is out of the office (returning 11/08/2013) (Bruce_Palmatier@vwr.com) 7. Re: Aspergillus tissue blocks for controls (Hans B Snyder) 8. RE: Aspergillus tissue blocks for controls (Diana McCaig) 9. FDA Disclaimer (Hannen, Valerie) 10. FW: The results of your email commands (Scott, Allison D) 11. Hot Histology Career Opportunities Alert from Pam Barker at RELIA Solutions!! Even if you are happy where you are take a look please we offer a 500.00 referral fee and it's almost time for Christmas shopping!!! 11/5/2013 (Pam Barker) 12. (no subject) (Richard Yeo) 13. Faxitron Xray digital imaging (Scott, Allison D) 14. RE: Faxitron Xray digital imaging (WILLIAM DESALVO) ---------------------------------------------------------------------- Message: 1 Date: Mon, 4 Nov 2013 13:38:25 -0500 From: "Lee & Peggy Wenk" Subject: Re: [Histonet] basement membranes To: "Edwards, Richard E." , Message-ID: <3BC14F9010C4499A88E5351B42787F0B@HP2010> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original Electron Microscope. Even if you did a periodic acid-methenamine silver stain (PASM, Jones), which in my opinion is the best histology stain, since it is a silver stain, you can get different thicknesses of basement membrane (bm) by leaving it in longer or shorter than is optimal for that patient's bm thickness. Peggy A. Wenk, HTL(ASCP)SLS -----Original Message----- From: Edwards, Richard E. Sent: Monday, November 04, 2013 9:39 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] basement membranes Best technique, tinctorial or otherwise of identifying, with a view to measuring their width, many thanks. Richard Edwards Leicester University U.K. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Mon, 4 Nov 2013 13:45:26 -0500 From: "Davis, Cassie" Subject: [Histonet] (no subject) To: "Vickroy.Jim@mhsil.com" Cc: "histonet@lists.utsouthwestern.edu" Message-ID: <08861B9CF6C7774E874635A4818AE37B01C9AC44F5@CHEXCMS01.one.ads.che.org> Content-Type: text/plain; charset="iso-8859-1" Jim, We get our Fungus, GMS Aspergillus controls (slides) and many of our hard to find control slides from Newcomer Supply 1-800-383-7799 or 608-831-7888 Cassandra Davis CDavis@che-east.org 302-575-8095 Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ------------------------------ Message: 3 Date: Mon, 4 Nov 2013 13:46:23 -0500 From: "Davis, Cassie" Subject: [Histonet] GMS fungus controls To: "Vickroy.Jim@mhsil.com" Cc: "histonet@lists.utsouthwestern.edu" Message-ID: <08861B9CF6C7774E874635A4818AE37B01C9AC44F9@CHEXCMS01.one.ads.che.org> Content-Type: text/plain; charset="iso-8859-1" Jim, We get our Fungus, GMS Aspergillus controls (slides) and many of our hard to find control slides from Newcomer Supply 1-800-383-7799 or 608-831-7888 Cassandra Davis CDavis@che-east.org 302-575-8095 Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ------------------------------ Message: 4 Date: Mon, 4 Nov 2013 15:07:06 -0500 From: Patrick Laurie Subject: Re: [Histonet] Aspergillus tissue blocks for controls To: "Vickroy, Jim" Cc: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hi Jim, I have found a couple of ways. First, if you are fortunate to have a micro department nearby, they can make a very nice one for you (a Histotip in Sakura's Histologic). Another method, which doesn't work the best, can be moldy orange peel. And the easiest, you can create your own (moldy sausage) or even take some human tissue (fresh lung works the best) and leave it with some moldy meat, then you can get a great one. Good luck! On Mon, Nov 4, 2013 at 10:38 AM, Vickroy, Jim wrote: > We are in desperate needs of obtaining GMS controls. In the old days we > had all the fungal specimens we needed. Today it is getting hard to find > these tissues or controls. Does anyone have any idea where we can get > tissue blocks with Aspergillus or does anyone have any other suggestions > about GMS controls. > > James Vickroy BS, HT(ASCP) > > Surgical and Autopsy Pathology Technical Supervisor > Memorial Medical Center > 217-788-4046 > > > ________________________________ > This message (including any attachments) contains confidential information > intended for a specific individual and purpose, and is protected by law. If > you are not the intended recipient, you should delete this message. Any > disclosure, copying, or distribution of this message, or the taking of any > action based on it, is strictly prohibited. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie(HT)ASCP QIHC Histology Manager Celligent Diagnostics, LLC 101 East W.T. Harris Blvd | Suite 1212 | Charlotte, NC 28262 Work: 704-970-3300 Cell: 704-266-0869 ------------------------------ Message: 5 Date: Mon, 4 Nov 2013 21:28:33 +0100 From: "P.E. Visser" Subject: [Histonet] Problems with H&E Bleeding To: Message-ID: <002301ced99c$7098c9f0$51ca5dd0$@xs4all.nl> Content-Type: text/plain; charset="us-ascii" Dear anna I had while working for Baker chemicals, a customer in Germany who had a similar problem there H&E turned purple. the red disappeared. the environment around the section contained little red droplets (only visible under high magnification). after some time (because at first they clamed noting was changed) it became clear that they skipped ethanol 100 % and just used the cheaper 96% instead. when evaluated they tested 3 slides and that worked. but after 300 - 500 slides the xylene was saturated with water. eosin being soluble in water migrates from the section to the water emulate in the xylene. so I would test the ethanol you use. 3 times 100% is in my view a must. I also on time in Scotland was confronted with xylene that was sold as non recycled, but in fact it was. and sadly the supplier used an open container to speed up cooling down. the rain was the source of the water. it lead to several problems and took a long time before the we could find the source of these problems. wish you success with solving yours. regards piet ------------------------------ Message: 6 Date: Mon, 4 Nov 2013 16:00:59 -0500 From: Bruce_Palmatier@vwr.com Subject: [Histonet] AUTO: Bruce Palmatier is out of the office (returning 11/08/2013) To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII I am out of the office until 11/08/2013. I will be out of the office from Nov 4- Nov 8. If you have a question about an order or need assistance placing an order, or if the matter is urgent, please call 877-881-1192 between 8am and 8pm and a VWR Healthcare Service associate will assist you. For other matters, I will respond to emails within 24 hours. If you require pricing information, quotes, product information, or other assistance, Tim Rafferty is the new VWR Healthcare Sales Rep who's taken my place. He can be reached at timothy_rafferty@vwr.com or (717) 668-9045. Thank You, Bruce Palmatier Market Portfolio Manager VWR Healthcare bruce_palmatier@vwr.com mobile: 484.319.5563 fax: 484-881-7307 Customer Service: 877.881.1192 Fax: 484.881.6486 Customer Service email: HEALTHCARESERVICE@VWR.COM Note: This is an automated response to your message "Histonet Digest, Vol 120, Issue 5" sent on 11/4/2013 1:06:11 PM. This is the only notification you will receive while this person is away. ------------------------------ Message: 7 Date: Mon, 4 Nov 2013 16:46:37 -0500 From: Hans B Snyder Subject: Re: [Histonet] Aspergillus tissue blocks for controls To: Patrick Laurie Cc: "histonet@lists.utsouthwestern.edu" , "Vickroy, Jim" Message-ID: Content-Type: text/plain; charset=ISO-8859-1 We have tried the moldy peel also but the pathologists did not like this and would not accept any controls of this form. I don't blame them they want to see it naturally occurring. Hans B Snyder Histologistics 60 Prescott Street Worcester, MA 01605 508-308-7800 hans@histologistics.com On Mon, Nov 4, 2013 at 3:07 PM, Patrick Laurie wrote: > Hi Jim, > > I have found a couple of ways. First, if you are fortunate to have a micro > department nearby, they can make a very nice one for you (a Histotip in > Sakura's Histologic). Another method, which doesn't work the best, can be > moldy orange peel. And the easiest, you can create your own (moldy > sausage) or even take some human tissue (fresh lung works the best) and > leave it with some moldy meat, then you can get a great one. > > Good luck! > > > On Mon, Nov 4, 2013 at 10:38 AM, Vickroy, Jim > wrote: > > > We are in desperate needs of obtaining GMS controls. In the old days we > > had all the fungal specimens we needed. Today it is getting hard to find > > these tissues or controls. Does anyone have any idea where we can get > > tissue blocks with Aspergillus or does anyone have any other suggestions > > about GMS controls. > > > > James Vickroy BS, HT(ASCP) > > > > Surgical and Autopsy Pathology Technical Supervisor > > Memorial Medical Center > > 217-788-4046 > > > > > > ________________________________ > > This message (including any attachments) contains confidential > information > > intended for a specific individual and purpose, and is protected by law. > If > > you are not the intended recipient, you should delete this message. Any > > disclosure, copying, or distribution of this message, or the taking of > any > > action based on it, is strictly prohibited. > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > -- > > Patrick Laurie(HT)ASCP QIHC > > Histology Manager > > Celligent Diagnostics, LLC > > 101 East W.T. Harris Blvd | Suite 1212 | Charlotte, NC 28262 > > Work: 704-970-3300 Cell: 704-266-0869 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 8 Date: Mon, 4 Nov 2013 22:31:18 +0000 From: Diana McCaig Subject: RE: [Histonet] Aspergillus tissue blocks for controls To: "'Hans B Snyder'" , Patrick Laurie Cc: "histonet@lists.utsouthwestern.edu" , "Vickroy, Jim" Message-ID: Content-Type: text/plain; charset="us-ascii" As well we have used mushroom and other moldy food products but it only demonstrates the mold and not tissue elements. But you can mince tissue and mix it with the fungus or mold so it one big segment and this gave more desirable results. Diana -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hans B Snyder Sent: November-04-13 4:47 PM To: Patrick Laurie Cc: histonet@lists.utsouthwestern.edu; Vickroy, Jim Subject: Re: [Histonet] Aspergillus tissue blocks for controls We have tried the moldy peel also but the pathologists did not like this and would not accept any controls of this form. I don't blame them they want to see it naturally occurring. Hans B Snyder Histologistics 60 Prescott Street Worcester, MA 01605 508-308-7800 hans@histologistics.com On Mon, Nov 4, 2013 at 3:07 PM, Patrick Laurie wrote: > Hi Jim, > > I have found a couple of ways. First, if you are fortunate to have a > micro department nearby, they can make a very nice one for you (a > Histotip in Sakura's Histologic). Another method, which doesn't work > the best, can be moldy orange peel. And the easiest, you can create > your own (moldy > sausage) or even take some human tissue (fresh lung works the best) > and leave it with some moldy meat, then you can get a great one. > > Good luck! > > > On Mon, Nov 4, 2013 at 10:38 AM, Vickroy, Jim > wrote: > > > We are in desperate needs of obtaining GMS controls. In the old days we > > had all the fungal specimens we needed. Today it is getting hard to find > > these tissues or controls. Does anyone have any idea where we can get > > tissue blocks with Aspergillus or does anyone have any other > > suggestions about GMS controls. > > > > James Vickroy BS, HT(ASCP) > > > > Surgical and Autopsy Pathology Technical Supervisor Memorial > > Medical Center > > 217-788-4046 > > > > > > ________________________________ > > This message (including any attachments) contains confidential > information > > intended for a specific individual and purpose, and is protected by law. > If > > you are not the intended recipient, you should delete this message. > > Any disclosure, copying, or distribution of this message, or the > > taking of > any > > action based on it, is strictly prohibited. > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > -- > > Patrick Laurie(HT)ASCP QIHC > > Histology Manager > > Celligent Diagnostics, LLC > > 101 East W.T. Harris Blvd | Suite 1212 | Charlotte, NC 28262 > > Work: 704-970-3300 Cell: 704-266-0869 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Tue, 5 Nov 2013 05:47:53 -0500 From: "Hannen, Valerie" Subject: [Histonet] FDA Disclaimer To: "Histonet Post (histonet@lists.utsouthwestern.edu)" Message-ID: <450B7A81EDA0C54E97C53D60F00776C3232B4BB696@isexstore03> Content-Type: text/plain; charset="us-ascii" Good Morning... I have a question about the FDA disclaimer for Immuno's. If we are not doing the staining of Immuno's in our lab, but our Pathologists are interpreting those that are stained at our reference lab, are we still required to put the FDA disclaimer on our Path reports for those antibodies that require the disclaimer? Thanks so much!! Valerie Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.hannen@parrishmed.com =================== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" =================== ------------------------------ Message: 10 Date: Tue, 5 Nov 2013 14:50:15 +0000 From: "Scott, Allison D" Subject: [Histonet] FW: The results of your email commands To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Sent: Tuesday, November 05, 2013 8:48 AM To: Scott, Allison D Subject: The results of your email commands The results of your email command are provided below. Attached is your original message. - Results: Ignoring non-text/plain MIME parts - Unprocessed: Sent: Tuesday, November 05, 2013 8:47 AM To: histonet-request@lists.utsouthwestern.edu Subject: FW: FaxitronX-Ray digital imaging From: Scott, Allison D Sent: Tuesday, November 05, 2013 8:46 AM To: histonet-request@lists.utsouthwestern.edu Subject: Faxitron/X-Ray digital imaging Hello to all in histoland. Our chief pathologist is interested in getting = a faxitron xray digital imaging machine. Is anyone out there using this te= chnology and can you suggest a good company in which to order one from. An= y help in this will be greatly appreciated. Allison Scott HT(ASCP) Supervisor, Histology Lab LBJ Hospital Harris Health System - Ignored: Office: 713-566-2148 Lab: 713-566-5287 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from = your computer system. To the extent the information in this e-mail and any attachments contain = protected health information as defined by the Health Insurance Portability = and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and = 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/o= r = privileged. This e-mail may also be confidential and/or privileged under = Texas law. The e-mail is for the use of only the individual or entity name= d = above. If you are not the intended recipient, or any authorized = representative of the intended recipient, you are hereby notified that any = review, dissemination or copying of this e-mail and its attachments is = strictly prohibited. - Done. CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. ------------------------------ Message: 11 Date: Tue, 5 Nov 2013 10:08:46 -0500 From: "Pam Barker" Subject: [Histonet] Hot Histology Career Opportunities Alert from Pam Barker at RELIA Solutions!! Even if you are happy where you are take a look please we offer a 500.00 referral fee and it's almost time for Christmas shopping!!! 11/5/2013 To: "Histonet" Message-ID: <00dc01ceda38$ecf3afa0$c6db0ee0$@earthlink.net> Content-Type: text/plain; charset="us-ascii" Hi Histonetters!! I hope you are off to the start of a great week on this beautiful Fall Day. I wanted to drop you a line about some of the opportunities that I am currently working on. These clients aren't just "kicking tires" they are ready to interview and ready to hire. If you are looking to make a change now or make a commitment now and the actual change after the holidays if you are the right person for my client they will do what it takes to get you I guarantee it!! So grab a cup of coffee or a mug of tea and something pumpkin flavored (isn't everything pumpkin flavored this time of year?) and please take a minute to peruse my list of current openings. All of the positions I work with are fulltime permanent positions with some of the best facilities Nationwide. My clients offer excellent compensation including competitive salaries, great benefits, relocation assistance/sign on bonuses. Here is a list of my current openings: HISTOLOGY SUPERVISORS/MANAGERS: WA OH MA GA Pathology Supervisor - Seattle, WA Lead Pathology Tech - East of Columbus, OH Histology Supervisor - Boston, MA Histology Supervisor - Atlanta, GA HISTOTECHNICIANS/HISTOTECHNOLOGISTS: VA OH NM MA Histology Tech - Roanoke, VA Histotechnician Day shift -East of Columbus, OH Grossing Histotechnician Dayshift - Las Cruces, NM Histotechnician - Nights - Nashville, TN Night Shift Grossing Histotech - Boston, MA If you or any of your friends would like more information on any of these positions or if you would like to discuss opportunities in other areas or future job searches please contact me. I would be more than happy to assist you. You can reach me at 866-607-3542 or relia1@earthlink.net Remember I offer over 20 years of recruiting and employment counseling experience, knowledgeable, confidential and responsive service to you and your friends and a permanent placement practice dedicated to the histology profession. I work with facilities nationwide and I will keep your resume confidential. I will only represent you to jobs you tell me you are interested in looking into. Remember. It never hurts to keep an eye open even if you are happy in your present job. Pam - 866-607-3542 (866-60RELIA) Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia ------------------------------ Message: 12 Date: Tue, 5 Nov 2013 10:17:26 -0500 From: "Richard Yeo" Subject: [Histonet] (no subject) To: Message-ID: <17E893BEFAF44C0EAD05AF8876EDAF80.MAI@foxmail.wchosp.org> Content-Type: text/plain; charset="utf-8" I have an abundance of fungus controls. I need afb and h.pylori. I would be me know an Thanks Richard Yeo ryeo@foxma ------------------------------ Message: 13 Date: Tue, 5 Nov 2013 16:00:11 +0000 From: "Scott, Allison D" Subject: [Histonet] Faxitron Xray digital imaging To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Hello to all in histoland. Our chief pathologist is interested in getting a faxitron xray digital imaging machine. Is anyone out there using this technology and can you suggest a company in which to order one from. Any help in this will be greatly appreciated. Allison Scott HT(ASCP) Supervisor, Histology Lab LBJ Hospital Harris Health System Office: 713-566-2148 Lab: 713-566-5287 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. ------------------------------ Message: 14 Date: Tue, 5 Nov 2013 09:47:16 -0700 From: WILLIAM DESALVO Subject: RE: [Histonet] Faxitron Xray digital imaging To: "Scott, Allison D" , histonet Message-ID: Content-Type: text/plain; charset="iso-8859-1" We have used the Faxitron instrument for the past 3 years at multiple sites. Faxitron creates a digital image of a specimen quickly and safely and a t multiple magnifications. The instrument is placed in either Surgery or Surgical Pathology (SP) and we use it primarily on breast cases to assist in orientation and gross dissection, identifying radioactive seeds or locating micro calcification. The instrument is easy to use, safe and you can connect to multiple LIS systems and a Hospital PACS. There is no code to charge for use when used in SP, only when used by Radiology or Surgery. There is only one company that manufactures and sells the instrument: Faxitron Bioptics, LLC Tucson, AZ 877-910-0030 The are willing to bring a unit to your site for demonstration and you may be able to talk them into leaving for a evaluation. William DeSalvo, BS HTL(ASCP) > From: Allison.Scott@harrishealth.org > To: histonet@lists.utsouthwestern.edu > Date: Tue, 5 Nov 2013 16:00:11 +0000 > Subject: [Histonet] Faxitron Xray digital imaging > > Hello to all in histoland. Our chief pathologist is interested in getting a faxitron xray digital imaging machine. Is anyone out there using this technology and can you suggest a company in which to order one from. Any help in this will be greatly appreciated. > > > > > Allison Scott HT(ASCP) > Supervisor, Histology Lab > LBJ Hospital > Harris Health System > Office: 713-566-2148 > Lab: 713-566-5287 > > > CONFIDENTIALITY NOTICE: > If you have received this e-mail in error, please immediately notify the > sender by return e-mail and delete this e-mail and any attachments from > your computer system. > > To the extent the information in this e-mail and any attachments contain > protected health information as defined by the Health Insurance Portability > and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and > 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or > privileged. This e-mail may also be confidential and/or privileged under > Texas law. The e-mail is for the use of only the individual or entity named > above. If you are not the intended recipient, or any authorized > representative of the intended recipient, you are hereby notified that any > review, dissemination or copying of this e-mail and its attachments is > strictly prohibited. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 120, Issue 6 **************************************** From TMcNemar <@t> lmhealth.org Wed Nov 6 04:37:13 2013 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Wed Nov 6 04:38:40 2013 Subject: [Histonet] FW: Fungus Controls In-Reply-To: References: Message-ID: I made some controls years ago that we are still using today. I used bread mold and streaked it out on a blood agar plate. Once the plate was covered, cut the agar into cubes, wrapped it in lens paper and processed. Cuts and stains beautifully. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tim Higgins Sent: Tuesday, November 05, 2013 2:04 PM To: histonet@lists.utsouthwestern.edu; Vickroy.Jim@mhsil.com Subject: [Histonet] FW: Fungus Controls I have used orange peel for years. I never had a problem with the pathologist not approving the control for use on patient stains. Its "naturally occurring", a petri dish would not be naturally occurring in my mind. Once I verified the orange peel does stain the fungus the same way it does in human tissue by doing a side by side staining the pathologist were fine with it. Actually happy I was able to save some money. Most controls you get from other source are not human either, usually it some type of animal. Try the orange peel. I did like the idea of using the some type of moldy meat or having your micro department do something for you!!!! I have never tried that, but if it works that would be awesome. Thanks, Timothy N. Higgins, HT (ASCP), QIHC > Message: 4 > Date: Mon, 4 Nov 2013 15:07:06 -0500 > From: Patrick Laurie > Subject: Re: [Histonet] Aspergillus tissue blocks for controls > To: "Vickroy, Jim" > Cc: "histonet@lists.utsouthwestern.edu" > > Message-ID: > > Content-Type: text/plain; charset=ISO-8859-1 > > Hi Jim, > > I have found a couple of ways. First, if you are fortunate to have a micro > department nearby, they can make a very nice one for you (a Histotip in > Sakura's Histologic). Another method, which doesn't work the best, can be > moldy orange peel. And the easiest, you can create your own (moldy > sausage) or even take some human tissue (fresh lung works the best) and > leave it with some moldy meat, then you can get a great one. > > Good luck! > > > On Mon, Nov 4, 2013 at 10:38 AM, Vickroy, Jim wrote: > > > We are in desperate needs of obtaining GMS controls. In the old days we > > had all the fungal specimens we needed. Today it is getting hard to find > > these tissues or controls. Does anyone have any idea where we can get > > tissue blocks with Aspergillus or does anyone have any other suggestions > > about GMS controls. > > > > James Vickroy BS, HT(ASCP) > > > > Surgical and Autopsy Pathology Technical Supervisor > > Memorial Medical Center > > 217-788-4046 > > > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From tahseen <@t> brain.net.pk Wed Nov 6 06:33:59 2013 From: tahseen <@t> brain.net.pk (tahseen) Date: Wed Nov 6 06:34:09 2013 Subject: [Histonet] CD 34 antibody abcam (ab963) Message-ID: Hi All, We used CD 34 antibody abcam (ab963)with detection kit enVision to stain our samples from rats and rabbits. We need to stain vessels only but macrophages, fibroblast, collagen all are stained in other hand known +ve control (Tonsil) is perform good. Please help us that what should we do for IHC staining of our samples. Best regards Muhammad Tahseen Sr.Supervisor Histology SKMCH&RC Lahore From Judith_Pardue <@t> memorial.org Wed Nov 6 09:38:53 2013 From: Judith_Pardue <@t> memorial.org (Pardue, Judith) Date: Wed Nov 6 09:38:58 2013 Subject: [Histonet] Water rinse for H&E's Message-ID: Does anyone use hot water rinses on their h&e slides. If we use cold water our slides are very light and inconsistent. We stain on an automatic stainer. Judith Pardue Histology Supervisor Memorial Health Care System judith_pardue@memorial.org This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. From rjbuesa <@t> yahoo.com Wed Nov 6 09:50:32 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Nov 6 09:50:39 2013 Subject: [Histonet] Water rinse for H&E's In-Reply-To: References: Message-ID: <1383753032.32943.YahooMailNeo@web163103.mail.bf1.yahoo.com> I do not, but it seems that you need to use hot water. If it works for you, keep doing it. ? The rationale behind this issue is that water after the staining is not just waterto wash the sections, but also to change the pH of the hematoxylin so the stain is, lets say, "developed" and this is a process temperature driven (the speed of the reaction is directly proportional to the temp.) and if you are having weak results with your running water (evidently cold) and good with hot water, this demonstrates that you need hot water in your specific circumstances. I hope I explained myself Ren? J. ________________________________ From: "Pardue, Judith" To: "histonet@lists.utsouthwestern.edu" Sent: Wednesday, November 6, 2013 10:38 AM Subject: [Histonet] Water rinse for H&E's Does anyone use hot water rinses on their h&e slides. If we use cold water our slides are very light and inconsistent. We stain on an automatic stainer. Judith Pardue Histology Supervisor Memorial Health Care System judith_pardue@memorial.org This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Terri.Brown <@t> Northside.com Wed Nov 6 11:20:48 2013 From: Terri.Brown <@t> Northside.com (Terri Brown) Date: Wed Nov 6 11:21:02 2013 Subject: [Histonet] Water rinse for H&E's In-Reply-To: References: Message-ID: <731941C266951A47BEF11E5EFAAED9C91EF5D2F4@nsmvexch01.northside.local> We use warm water rinses for our H&E slides on our automatic stainers. Terri H. Brown,, HT (ASCP) Pathology Laboratory Manager Northside Hospital Atlanta terri.brown@northside.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pardue, Judith Sent: Wednesday, November 06, 2013 10:39 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Water rinse for H&E's Does anyone use hot water rinses on their h&e slides. If we use cold water our slides are very light and inconsistent. We stain on an automatic stainer. Judith Pardue Histology Supervisor Memorial Health Care System judith_pardue@memorial.org This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by Northside Hospital. It may contain information that is confidential, privileged, proprietary, or otherwise legally exempt from disclosure. If you are not the intended recipient, you are hereby notified that you are not authorized to read, print, retain, copy or disseminate this message, any part of it, or any attachments. If you have received this message in error, please delete this message and any attachments from your system without reading the content and notify the sender immediately of the inadvertent transmission. There is no intent on the part of the sender to waive any privilege. From jclark <@t> pcnm.com Wed Nov 6 11:22:14 2013 From: jclark <@t> pcnm.com (Joanne Clark) Date: Wed Nov 6 11:22:21 2013 Subject: [Histonet] Biopsy Problems In-Reply-To: <20131105180459.25E77A7D8D5@mx10.myoutlookonline.com> References: <20131105180459.25E77A7D8D5@mx10.myoutlookonline.com> Message-ID: <0494A7D4E8CC254EA2FB81464982E378B4ACACEE@S10MAILD001N3.SH10.lan> Hi Fellow Histonetters, we have started having this problem with our G.I. biopsies, and not all of them, just the odd one here and there. After staining, when looking at it on the scope, you can't get it to focus in one plane. It's not cutting artifact, because we have recut the block making sure it is well cooled etc. before picking up and staining the section. The cells, when in focus, look fixed and properly processed, and the staining is the way it should be. I have no idea what is causing this artifact. Has anyone else seen this and can shed some light on what is causing it? Joanne Clark, HT Director of Histology Pathology Consultants of New Mexico Disclaimer: This electronic message may contain information that is proprietary, confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From JMcCarty <@t> its.jnj.com Wed Nov 6 12:05:01 2013 From: JMcCarty <@t> its.jnj.com (McCarty, Jean [JRDUS]) Date: Wed Nov 6 12:05:16 2013 Subject: [Histonet] unsubscribe Message-ID: -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Wednesday, November 06, 2013 1:02 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 120, Issue 7 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. FW: Fungus Controls (Tim Higgins) 2. RE: Histonet Digest, Vol 120, Issue 6 (Gray, Ed) 3. RE: FDA Disclaimer (Hannen, Valerie) (Jean Wood) 4. RE: FW: Fungus Controls (Tom McNemar) 5. CD 34 antibody abcam (ab963) (tahseen) 6. Water rinse for H&E's (Pardue, Judith) 7. Re: Water rinse for H&E's (Rene J Buesa) 8. RE: Water rinse for H&E's (Terri Brown) 9. Biopsy Problems (Joanne Clark) ---------------------------------------------------------------------- Message: 1 Date: Tue, 5 Nov 2013 13:03:54 -0600 From: Tim Higgins Subject: [Histonet] FW: Fungus Controls To: "histonet@lists.utsouthwestern.edu" , "Vickroy.Jim@mhsil.com" Message-ID: Content-Type: text/plain; charset="iso-8859-1" I have used orange peel for years. I never had a problem with the pathologist not approving the control for use on patient stains. Its "naturally occurring", a petri dish would not be naturally occurring in my mind. Once I verified the orange peel does stain the fungus the same way it does in human tissue by doing a side by side staining the pathologist were fine with it. Actually happy I was able to save some money. Most controls you get from other source are not human either, usually it some type of animal. Try the orange peel. I did like the idea of using the some type of moldy meat or having your micro department do something for you!!!! I have never tried that, but if it works that would be awesome. Thanks, Timothy N. Higgins, HT (ASCP), QIHC > Message: 4 > Date: Mon, 4 Nov 2013 15:07:06 -0500 > From: Patrick Laurie > Subject: Re: [Histonet] Aspergillus tissue blocks for controls > To: "Vickroy, Jim" > Cc: "histonet@lists.utsouthwestern.edu" > > Message-ID: > > Content-Type: text/plain; charset=ISO-8859-1 > > Hi Jim, > > I have found a couple of ways. First, if you are fortunate to have a micro > department nearby, they can make a very nice one for you (a Histotip in > Sakura's Histologic). Another method, which doesn't work the best, can be > moldy orange peel. And the easiest, you can create your own (moldy > sausage) or even take some human tissue (fresh lung works the best) and > leave it with some moldy meat, then you can get a great one. > > Good luck! > > > On Mon, Nov 4, 2013 at 10:38 AM, Vickroy, Jim wrote: > > > We are in desperate needs of obtaining GMS controls. In the old days we > > had all the fungal specimens we needed. Today it is getting hard to find > > these tissues or controls. Does anyone have any idea where we can get > > tissue blocks with Aspergillus or does anyone have any other suggestions > > about GMS controls. > > > > James Vickroy BS, HT(ASCP) > > > > Surgical and Autopsy Pathology Technical Supervisor > > Memorial Medical Center > > 217-788-4046 > > > > ------------------------------ Message: 2 Date: Tue, 5 Nov 2013 19:20:42 +0000 From: "Gray, Ed" Subject: [Histonet] RE: Histonet Digest, Vol 120, Issue 6 To: "histonet@lists.utsouthwestern.edu" Message-ID: <6a889df9e216449bbac73ab5d795aa71@BN1PR05MB406.namprd05.prod.outlook.com> Content-Type: text/plain; charset="us-ascii" You actually need the performing lab's disclaimer. We've sent stains and other procedures to several other labs. We refer to the disclaimers as ASR's (analyte specific reagents) and build templates for each lab's specific comments in our APLIS. Ed Gray | Pathology IT Analyst | Phone: 304-293-2945 | Fax 304-293-1627 | WVU Healthcare l egray@wvuhealthcare.com Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. Message: 9 Date: Tue, 5 Nov 2013 05:47:53 -0500 From: "Hannen, Valerie" Subject: [Histonet] FDA Disclaimer To: "Histonet Post (histonet@lists.utsouthwestern.edu)" Message-ID: <450B7A81EDA0C54E97C53D60F00776C3232B4BB696@isexstore03> Content-Type: text/plain; charset="us-ascii" Good Morning... I have a question about the FDA disclaimer for Immuno's. If we are not doing the staining of Immuno's in our lab, but our Pathologists are interpreting those that are stained at our reference lab, are we still required to put the FDA disclaimer on our Path reports for those antibodies that require the disclaimer? Thanks so much!! Valerie Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.hannen@parrishmed.com =================== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" =================== ------------------------------ Message: 3 Date: Tue, 5 Nov 2013 13:41:14 -0800 From: Jean Wood Subject: [Histonet] RE: FDA Disclaimer (Hannen, Valerie) To: "histonet@lists.utsouthwestern.edu" Message-ID: <87A4EF3F1FBA9D42A7D1EC82F031C0B80A4FD115D6@cliff> Content-Type: text/plain; charset="us-ascii" Valerie, If your pathologist is reading your IHC send-outs then he is most likely contracted through that third party company (he gets PC compensation) and they do the technicial work. In our case, our pathologist signs out our routine H&E Final Surgical Pathology Report and when he reads the IHC he uses their report portal to issue those results using their letterhead with the FDA disclaimer attached at the end of the report. Sometimes he may incorporate the IHC results in the original report as revised but we always piggy back the third party IHC report (with digital pathology images) to the original. It might be best to contact the IHC company representative and find out excatly who is legally responsible for the disclaimer - the technical service provider or the proffesional. Jean Wood BS, HT Fairchild Medical Center Pathology Dept. Ph:530.841.6243 Fax:530.841.6232 jwood@fairchildmed.org ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu [histonet-request@lists.utsouthwestern.edu] Sent: Tuesday, November 05, 2013 10:05 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 120, Issue 6 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: basement membranes (Lee & Peggy Wenk) 2. (no subject) (Davis, Cassie) 3. GMS fungus controls (Davis, Cassie) 4. Re: Aspergillus tissue blocks for controls (Patrick Laurie) 5. Problems with H&E Bleeding (P.E. Visser) 6. AUTO: Bruce Palmatier is out of the office (returning 11/08/2013) (Bruce_Palmatier@vwr.com) 7. Re: Aspergillus tissue blocks for controls (Hans B Snyder) 8. RE: Aspergillus tissue blocks for controls (Diana McCaig) 9. FDA Disclaimer (Hannen, Valerie) 10. FW: The results of your email commands (Scott, Allison D) 11. Hot Histology Career Opportunities Alert from Pam Barker at RELIA Solutions!! Even if you are happy where you are take a look please we offer a 500.00 referral fee and it's almost time for Christmas shopping!!! 11/5/2013 (Pam Barker) 12. (no subject) (Richard Yeo) 13. Faxitron Xray digital imaging (Scott, Allison D) 14. RE: Faxitron Xray digital imaging (WILLIAM DESALVO) ---------------------------------------------------------------------- Message: 1 Date: Mon, 4 Nov 2013 13:38:25 -0500 From: "Lee & Peggy Wenk" Subject: Re: [Histonet] basement membranes To: "Edwards, Richard E." , Message-ID: <3BC14F9010C4499A88E5351B42787F0B@HP2010> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original Electron Microscope. Even if you did a periodic acid-methenamine silver stain (PASM, Jones), which in my opinion is the best histology stain, since it is a silver stain, you can get different thicknesses of basement membrane (bm) by leaving it in longer or shorter than is optimal for that patient's bm thickness. Peggy A. Wenk, HTL(ASCP)SLS -----Original Message----- From: Edwards, Richard E. Sent: Monday, November 04, 2013 9:39 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] basement membranes Best technique, tinctorial or otherwise of identifying, with a view to measuring their width, many thanks. Richard Edwards Leicester University U.K. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Mon, 4 Nov 2013 13:45:26 -0500 From: "Davis, Cassie" Subject: [Histonet] (no subject) To: "Vickroy.Jim@mhsil.com" Cc: "histonet@lists.utsouthwestern.edu" Message-ID: <08861B9CF6C7774E874635A4818AE37B01C9AC44F5@CHEXCMS01.one.ads.che.org> Content-Type: text/plain; charset="iso-8859-1" Jim, We get our Fungus, GMS Aspergillus controls (slides) and many of our hard to find control slides from Newcomer Supply 1-800-383-7799 or 608-831-7888 Cassandra Davis CDavis@che-east.org 302-575-8095 Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ------------------------------ Message: 3 Date: Mon, 4 Nov 2013 13:46:23 -0500 From: "Davis, Cassie" Subject: [Histonet] GMS fungus controls To: "Vickroy.Jim@mhsil.com" Cc: "histonet@lists.utsouthwestern.edu" Message-ID: <08861B9CF6C7774E874635A4818AE37B01C9AC44F9@CHEXCMS01.one.ads.che.org> Content-Type: text/plain; charset="iso-8859-1" Jim, We get our Fungus, GMS Aspergillus controls (slides) and many of our hard to find control slides from Newcomer Supply 1-800-383-7799 or 608-831-7888 Cassandra Davis CDavis@che-east.org 302-575-8095 Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ------------------------------ Message: 4 Date: Mon, 4 Nov 2013 15:07:06 -0500 From: Patrick Laurie Subject: Re: [Histonet] Aspergillus tissue blocks for controls To: "Vickroy, Jim" Cc: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hi Jim, I have found a couple of ways. First, if you are fortunate to have a micro department nearby, they can make a very nice one for you (a Histotip in Sakura's Histologic). Another method, which doesn't work the best, can be moldy orange peel. And the easiest, you can create your own (moldy sausage) or even take some human tissue (fresh lung works the best) and leave it with some moldy meat, then you can get a great one. Good luck! On Mon, Nov 4, 2013 at 10:38 AM, Vickroy, Jim wrote: > We are in desperate needs of obtaining GMS controls. In the old days we > had all the fungal specimens we needed. Today it is getting hard to find > these tissues or controls. Does anyone have any idea where we can get > tissue blocks with Aspergillus or does anyone have any other suggestions > about GMS controls. > > James Vickroy BS, HT(ASCP) > > Surgical and Autopsy Pathology Technical Supervisor > Memorial Medical Center > 217-788-4046 > > > ________________________________ > This message (including any attachments) contains confidential information > intended for a specific individual and purpose, and is protected by law. If > you are not the intended recipient, you should delete this message. Any > disclosure, copying, or distribution of this message, or the taking of any > action based on it, is strictly prohibited. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie(HT)ASCP QIHC Histology Manager Celligent Diagnostics, LLC 101 East W.T. Harris Blvd | Suite 1212 | Charlotte, NC 28262 Work: 704-970-3300 Cell: 704-266-0869 ------------------------------ Message: 5 Date: Mon, 4 Nov 2013 21:28:33 +0100 From: "P.E. Visser" Subject: [Histonet] Problems with H&E Bleeding To: Message-ID: <002301ced99c$7098c9f0$51ca5dd0$@xs4all.nl> Content-Type: text/plain; charset="us-ascii" Dear anna I had while working for Baker chemicals, a customer in Germany who had a similar problem there H&E turned purple. the red disappeared. the environment around the section contained little red droplets (only visible under high magnification). after some time (because at first they clamed noting was changed) it became clear that they skipped ethanol 100 % and just used the cheaper 96% instead. when evaluated they tested 3 slides and that worked. but after 300 - 500 slides the xylene was saturated with water. eosin being soluble in water migrates from the section to the water emulate in the xylene. so I would test the ethanol you use. 3 times 100% is in my view a must. I also on time in Scotland was confronted with xylene that was sold as non recycled, but in fact it was. and sadly the supplier used an open container to speed up cooling down. the rain was the source of the water. it lead to several problems and took a long time before the we could find the source of these problems. wish you success with solving yours. regards piet ------------------------------ Message: 6 Date: Mon, 4 Nov 2013 16:00:59 -0500 From: Bruce_Palmatier@vwr.com Subject: [Histonet] AUTO: Bruce Palmatier is out of the office (returning 11/08/2013) To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII I am out of the office until 11/08/2013. I will be out of the office from Nov 4- Nov 8. If you have a question about an order or need assistance placing an order, or if the matter is urgent, please call 877-881-1192 between 8am and 8pm and a VWR Healthcare Service associate will assist you. For other matters, I will respond to emails within 24 hours. If you require pricing information, quotes, product information, or other assistance, Tim Rafferty is the new VWR Healthcare Sales Rep who's taken my place. He can be reached at timothy_rafferty@vwr.com or (717) 668-9045. Thank You, Bruce Palmatier Market Portfolio Manager VWR Healthcare bruce_palmatier@vwr.com mobile: 484.319.5563 fax: 484-881-7307 Customer Service: 877.881.1192 Fax: 484.881.6486 Customer Service email: HEALTHCARESERVICE@VWR.COM Note: This is an automated response to your message "Histonet Digest, Vol 120, Issue 5" sent on 11/4/2013 1:06:11 PM. This is the only notification you will receive while this person is away. ------------------------------ Message: 7 Date: Mon, 4 Nov 2013 16:46:37 -0500 From: Hans B Snyder Subject: Re: [Histonet] Aspergillus tissue blocks for controls To: Patrick Laurie Cc: "histonet@lists.utsouthwestern.edu" , "Vickroy, Jim" Message-ID: Content-Type: text/plain; charset=ISO-8859-1 We have tried the moldy peel also but the pathologists did not like this and would not accept any controls of this form. I don't blame them they want to see it naturally occurring. Hans B Snyder Histologistics 60 Prescott Street Worcester, MA 01605 508-308-7800 hans@histologistics.com On Mon, Nov 4, 2013 at 3:07 PM, Patrick Laurie wrote: > Hi Jim, > > I have found a couple of ways. First, if you are fortunate to have a micro > department nearby, they can make a very nice one for you (a Histotip in > Sakura's Histologic). Another method, which doesn't work the best, can be > moldy orange peel. And the easiest, you can create your own (moldy > sausage) or even take some human tissue (fresh lung works the best) and > leave it with some moldy meat, then you can get a great one. > > Good luck! > > > On Mon, Nov 4, 2013 at 10:38 AM, Vickroy, Jim > wrote: > > > We are in desperate needs of obtaining GMS controls. In the old days we > > had all the fungal specimens we needed. Today it is getting hard to find > > these tissues or controls. Does anyone have any idea where we can get > > tissue blocks with Aspergillus or does anyone have any other suggestions > > about GMS controls. > > > > James Vickroy BS, HT(ASCP) > > > > Surgical and Autopsy Pathology Technical Supervisor > > Memorial Medical Center > > 217-788-4046 > > > > > > ________________________________ > > This message (including any attachments) contains confidential > information > > intended for a specific individual and purpose, and is protected by law. > If > > you are not the intended recipient, you should delete this message. Any > > disclosure, copying, or distribution of this message, or the taking of > any > > action based on it, is strictly prohibited. > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > -- > > Patrick Laurie(HT)ASCP QIHC > > Histology Manager > > Celligent Diagnostics, LLC > > 101 East W.T. Harris Blvd | Suite 1212 | Charlotte, NC 28262 > > Work: 704-970-3300 Cell: 704-266-0869 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 8 Date: Mon, 4 Nov 2013 22:31:18 +0000 From: Diana McCaig Subject: RE: [Histonet] Aspergillus tissue blocks for controls To: "'Hans B Snyder'" , Patrick Laurie Cc: "histonet@lists.utsouthwestern.edu" , "Vickroy, Jim" Message-ID: Content-Type: text/plain; charset="us-ascii" As well we have used mushroom and other moldy food products but it only demonstrates the mold and not tissue elements. But you can mince tissue and mix it with the fungus or mold so it one big segment and this gave more desirable results. Diana -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hans B Snyder Sent: November-04-13 4:47 PM To: Patrick Laurie Cc: histonet@lists.utsouthwestern.edu; Vickroy, Jim Subject: Re: [Histonet] Aspergillus tissue blocks for controls We have tried the moldy peel also but the pathologists did not like this and would not accept any controls of this form. I don't blame them they want to see it naturally occurring. Hans B Snyder Histologistics 60 Prescott Street Worcester, MA 01605 508-308-7800 hans@histologistics.com On Mon, Nov 4, 2013 at 3:07 PM, Patrick Laurie wrote: > Hi Jim, > > I have found a couple of ways. First, if you are fortunate to have a > micro department nearby, they can make a very nice one for you (a > Histotip in Sakura's Histologic). Another method, which doesn't work > the best, can be moldy orange peel. And the easiest, you can create > your own (moldy > sausage) or even take some human tissue (fresh lung works the best) > and leave it with some moldy meat, then you can get a great one. > > Good luck! > > > On Mon, Nov 4, 2013 at 10:38 AM, Vickroy, Jim > wrote: > > > We are in desperate needs of obtaining GMS controls. In the old days we > > had all the fungal specimens we needed. Today it is getting hard to find > > these tissues or controls. Does anyone have any idea where we can get > > tissue blocks with Aspergillus or does anyone have any other > > suggestions about GMS controls. > > > > James Vickroy BS, HT(ASCP) > > > > Surgical and Autopsy Pathology Technical Supervisor Memorial > > Medical Center > > 217-788-4046 > > > > > > ________________________________ > > This message (including any attachments) contains confidential > information > > intended for a specific individual and purpose, and is protected by law. > If > > you are not the intended recipient, you should delete this message. > > Any disclosure, copying, or distribution of this message, or the > > taking of > any > > action based on it, is strictly prohibited. > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > -- > > Patrick Laurie(HT)ASCP QIHC > > Histology Manager > > Celligent Diagnostics, LLC > > 101 East W.T. Harris Blvd | Suite 1212 | Charlotte, NC 28262 > > Work: 704-970-3300 Cell: 704-266-0869 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Tue, 5 Nov 2013 05:47:53 -0500 From: "Hannen, Valerie" Subject: [Histonet] FDA Disclaimer To: "Histonet Post (histonet@lists.utsouthwestern.edu)" Message-ID: <450B7A81EDA0C54E97C53D60F00776C3232B4BB696@isexstore03> Content-Type: text/plain; charset="us-ascii" Good Morning... I have a question about the FDA disclaimer for Immuno's. If we are not doing the staining of Immuno's in our lab, but our Pathologists are interpreting those that are stained at our reference lab, are we still required to put the FDA disclaimer on our Path reports for those antibodies that require the disclaimer? Thanks so much!! Valerie Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.hannen@parrishmed.com =================== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" =================== ------------------------------ Message: 10 Date: Tue, 5 Nov 2013 14:50:15 +0000 From: "Scott, Allison D" Subject: [Histonet] FW: The results of your email commands To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Sent: Tuesday, November 05, 2013 8:48 AM To: Scott, Allison D Subject: The results of your email commands The results of your email command are provided below. Attached is your original message. - Results: Ignoring non-text/plain MIME parts - Unprocessed: Sent: Tuesday, November 05, 2013 8:47 AM To: histonet-request@lists.utsouthwestern.edu Subject: FW: FaxitronX-Ray digital imaging From: Scott, Allison D Sent: Tuesday, November 05, 2013 8:46 AM To: histonet-request@lists.utsouthwestern.edu Subject: Faxitron/X-Ray digital imaging Hello to all in histoland. Our chief pathologist is interested in getting = a faxitron xray digital imaging machine. Is anyone out there using this te= chnology and can you suggest a good company in which to order one from. An= y help in this will be greatly appreciated. Allison Scott HT(ASCP) Supervisor, Histology Lab LBJ Hospital Harris Health System - Ignored: Office: 713-566-2148 Lab: 713-566-5287 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from = your computer system. To the extent the information in this e-mail and any attachments contain = protected health information as defined by the Health Insurance Portability = and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and = 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/o= r = privileged. This e-mail may also be confidential and/or privileged under = Texas law. The e-mail is for the use of only the individual or entity name= d = above. If you are not the intended recipient, or any authorized = representative of the intended recipient, you are hereby notified that any = review, dissemination or copying of this e-mail and its attachments is = strictly prohibited. - Done. CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. ------------------------------ Message: 11 Date: Tue, 5 Nov 2013 10:08:46 -0500 From: "Pam Barker" Subject: [Histonet] Hot Histology Career Opportunities Alert from Pam Barker at RELIA Solutions!! Even if you are happy where you are take a look please we offer a 500.00 referral fee and it's almost time for Christmas shopping!!! 11/5/2013 To: "Histonet" Message-ID: <00dc01ceda38$ecf3afa0$c6db0ee0$@earthlink.net> Content-Type: text/plain; charset="us-ascii" Hi Histonetters!! I hope you are off to the start of a great week on this beautiful Fall Day. I wanted to drop you a line about some of the opportunities that I am currently working on. These clients aren't just "kicking tires" they are ready to interview and ready to hire. If you are looking to make a change now or make a commitment now and the actual change after the holidays if you are the right person for my client they will do what it takes to get you I guarantee it!! So grab a cup of coffee or a mug of tea and something pumpkin flavored (isn't everything pumpkin flavored this time of year?) and please take a minute to peruse my list of current openings. All of the positions I work with are fulltime permanent positions with some of the best facilities Nationwide. My clients offer excellent compensation including competitive salaries, great benefits, relocation assistance/sign on bonuses. Here is a list of my current openings: HISTOLOGY SUPERVISORS/MANAGERS: WA OH MA GA Pathology Supervisor - Seattle, WA Lead Pathology Tech - East of Columbus, OH Histology Supervisor - Boston, MA Histology Supervisor - Atlanta, GA HISTOTECHNICIANS/HISTOTECHNOLOGISTS: VA OH NM MA Histology Tech - Roanoke, VA Histotechnician Day shift -East of Columbus, OH Grossing Histotechnician Dayshift - Las Cruces, NM Histotechnician - Nights - Nashville, TN Night Shift Grossing Histotech - Boston, MA If you or any of your friends would like more information on any of these positions or if you would like to discuss opportunities in other areas or future job searches please contact me. I would be more than happy to assist you. You can reach me at 866-607-3542 or relia1@earthlink.net Remember I offer over 20 years of recruiting and employment counseling experience, knowledgeable, confidential and responsive service to you and your friends and a permanent placement practice dedicated to the histology profession. I work with facilities nationwide and I will keep your resume confidential. I will only represent you to jobs you tell me you are interested in looking into. Remember. It never hurts to keep an eye open even if you are happy in your present job. Pam - 866-607-3542 (866-60RELIA) Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia ------------------------------ Message: 12 Date: Tue, 5 Nov 2013 10:17:26 -0500 From: "Richard Yeo" Subject: [Histonet] (no subject) To: Message-ID: <17E893BEFAF44C0EAD05AF8876EDAF80.MAI@foxmail.wchosp.org> Content-Type: text/plain; charset="utf-8" I have an abundance of fungus controls. I need afb and h.pylori. I would be me know an Thanks Richard Yeo ryeo@foxma ------------------------------ Message: 13 Date: Tue, 5 Nov 2013 16:00:11 +0000 From: "Scott, Allison D" Subject: [Histonet] Faxitron Xray digital imaging To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Hello to all in histoland. Our chief pathologist is interested in getting a faxitron xray digital imaging machine. Is anyone out there using this technology and can you suggest a company in which to order one from. Any help in this will be greatly appreciated. Allison Scott HT(ASCP) Supervisor, Histology Lab LBJ Hospital Harris Health System Office: 713-566-2148 Lab: 713-566-5287 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. ------------------------------ Message: 14 Date: Tue, 5 Nov 2013 09:47:16 -0700 From: WILLIAM DESALVO Subject: RE: [Histonet] Faxitron Xray digital imaging To: "Scott, Allison D" , histonet Message-ID: Content-Type: text/plain; charset="iso-8859-1" We have used the Faxitron instrument for the past 3 years at multiple sites. Faxitron creates a digital image of a specimen quickly and safely and a t multiple magnifications. The instrument is placed in either Surgery or Surgical Pathology (SP) and we use it primarily on breast cases to assist in orientation and gross dissection, identifying radioactive seeds or locating micro calcification. The instrument is easy to use, safe and you can connect to multiple LIS systems and a Hospital PACS. There is no code to charge for use when used in SP, only when used by Radiology or Surgery. There is only one company that manufactures and sells the instrument: Faxitron Bioptics, LLC Tucson, AZ 877-910-0030 The are willing to bring a unit to your site for demonstration and you may be able to talk them into leaving for a evaluation. William DeSalvo, BS HTL(ASCP) > From: Allison.Scott@harrishealth.org > To: histonet@lists.utsouthwestern.edu > Date: Tue, 5 Nov 2013 16:00:11 +0000 > Subject: [Histonet] Faxitron Xray digital imaging > > Hello to all in histoland. Our chief pathologist is interested in getting a faxitron xray digital imaging machine. Is anyone out there using this technology and can you suggest a company in which to order one from. Any help in this will be greatly appreciated. > > > > > Allison Scott HT(ASCP) > Supervisor, Histology Lab > LBJ Hospital > Harris Health System > Office: 713-566-2148 > Lab: 713-566-5287 > > > CONFIDENTIALITY NOTICE: > If you have received this e-mail in error, please immediately notify the > sender by return e-mail and delete this e-mail and any attachments from > your computer system. > > To the extent the information in this e-mail and any attachments contain > protected health information as defined by the Health Insurance Portability > and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and > 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or > privileged. This e-mail may also be confidential and/or privileged under > Texas law. The e-mail is for the use of only the individual or entity named > above. If you are not the intended recipient, or any authorized > representative of the intended recipient, you are hereby notified that any > review, dissemination or copying of this e-mail and its attachments is > strictly prohibited. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 120, Issue 6 **************************************** ------------------------------ Message: 4 Date: Wed, 6 Nov 2013 05:37:13 -0500 From: Tom McNemar Subject: RE: [Histonet] FW: Fungus Controls To: 'Tim Higgins' , "histonet@lists.utsouthwestern.edu" , "Vickroy.Jim@mhsil.com" Message-ID: Content-Type: text/plain; charset="us-ascii" I made some controls years ago that we are still using today. I used bread mold and streaked it out on a blood agar plate. Once the plate was covered, cut the agar into cubes, wrapped it in lens paper and processed. Cuts and stains beautifully. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tim Higgins Sent: Tuesday, November 05, 2013 2:04 PM To: histonet@lists.utsouthwestern.edu; Vickroy.Jim@mhsil.com Subject: [Histonet] FW: Fungus Controls I have used orange peel for years. I never had a problem with the pathologist not approving the control for use on patient stains. Its "naturally occurring", a petri dish would not be naturally occurring in my mind. Once I verified the orange peel does stain the fungus the same way it does in human tissue by doing a side by side staining the pathologist were fine with it. Actually happy I was able to save some money. Most controls you get from other source are not human either, usually it some type of animal. Try the orange peel. I did like the idea of using the some type of moldy meat or having your micro department do something for you!!!! I have never tried that, but if it works that would be awesome. Thanks, Timothy N. Higgins, HT (ASCP), QIHC > Message: 4 > Date: Mon, 4 Nov 2013 15:07:06 -0500 > From: Patrick Laurie > Subject: Re: [Histonet] Aspergillus tissue blocks for controls > To: "Vickroy, Jim" > Cc: "histonet@lists.utsouthwestern.edu" > > Message-ID: > > Content-Type: text/plain; charset=ISO-8859-1 > > Hi Jim, > > I have found a couple of ways. First, if you are fortunate to have a micro > department nearby, they can make a very nice one for you (a Histotip in > Sakura's Histologic). Another method, which doesn't work the best, can be > moldy orange peel. And the easiest, you can create your own (moldy > sausage) or even take some human tissue (fresh lung works the best) and > leave it with some moldy meat, then you can get a great one. > > Good luck! > > > On Mon, Nov 4, 2013 at 10:38 AM, Vickroy, Jim wrote: > > > We are in desperate needs of obtaining GMS controls. In the old days we > > had all the fungal specimens we needed. Today it is getting hard to find > > these tissues or controls. Does anyone have any idea where we can get > > tissue blocks with Aspergillus or does anyone have any other suggestions > > about GMS controls. > > > > James Vickroy BS, HT(ASCP) > > > > Surgical and Autopsy Pathology Technical Supervisor > > Memorial Medical Center > > 217-788-4046 > > > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ------------------------------ Message: 5 Date: Wed, 06 Nov 2013 17:33:59 +0500 From: tahseen Subject: [Histonet] CD 34 antibody abcam (ab963) To: Message-ID: Content-Type: text/plain; charset=UTF-8; format=flowed Hi All, We used CD 34 antibody abcam (ab963)with detection kit enVision to stain our samples from rats and rabbits. We need to stain vessels only but macrophages, fibroblast, collagen all are stained in other hand known +ve control (Tonsil) is perform good. Please help us that what should we do for IHC staining of our samples. Best regards Muhammad Tahseen Sr.Supervisor Histology SKMCH&RC Lahore ------------------------------ Message: 6 Date: Wed, 6 Nov 2013 15:38:53 +0000 From: "Pardue, Judith" Subject: [Histonet] Water rinse for H&E's To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Does anyone use hot water rinses on their h&e slides. If we use cold water our slides are very light and inconsistent. We stain on an automatic stainer. Judith Pardue Histology Supervisor Memorial Health Care System judith_pardue@memorial.org This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. ------------------------------ Message: 7 Date: Wed, 6 Nov 2013 07:50:32 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] Water rinse for H&E's To: "Pardue, Judith" , "histonet@lists.utsouthwestern.edu" Message-ID: <1383753032.32943.YahooMailNeo@web163103.mail.bf1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I do not, but it seems that you need to use hot water. If it works for you, keep doing it. ? The rationale behind this issue is that water after the staining is not just waterto wash the sections, but also to change the pH of the hematoxylin so the stain is, lets say, "developed" and this is a process temperature driven (the speed of the reaction is directly proportional to the temp.) and if you are having weak results with your running water (evidently cold) and good with hot water, this demonstrates that you need hot water in your specific circumstances. I hope I explained myself Ren? J. ________________________________ From: "Pardue, Judith" To: "histonet@lists.utsouthwestern.edu" Sent: Wednesday, November 6, 2013 10:38 AM Subject: [Histonet] Water rinse for H&E's Does anyone use hot water rinses on their h&e slides. If we use cold water our slides are very light and inconsistent. We stain on an automatic stainer. Judith Pardue Histology Supervisor Memorial Health Care System judith_pardue@memorial.org This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Wed, 6 Nov 2013 12:20:48 -0500 From: "Terri Brown" Subject: RE: [Histonet] Water rinse for H&E's To: "Pardue, Judith" , Message-ID: <731941C266951A47BEF11E5EFAAED9C91EF5D2F4@nsmvexch01.northside.local> Content-Type: text/plain;charset="us-ascii" We use warm water rinses for our H&E slides on our automatic stainers. Terri H. Brown,, HT (ASCP) Pathology Laboratory Manager Northside Hospital Atlanta terri.brown@northside.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pardue, Judith Sent: Wednesday, November 06, 2013 10:39 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Water rinse for H&E's Does anyone use hot water rinses on their h&e slides. If we use cold water our slides are very light and inconsistent. We stain on an automatic stainer. Judith Pardue Histology Supervisor Memorial Health Care System judith_pardue@memorial.org This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by Northside Hospital. It may contain information that is confidential, privileged, proprietary, or otherwise legally exempt from disclosure. If you are not the intended recipient, you are hereby notified that you are not authorized to read, print, retain, copy or disseminate this message, any part of it, or any attachments. If you have received this message in error, please delete this message and any attachments from your system without reading the content and notify the sender immediately of the inadvertent transmission. There is no intent on the part of the sender to waive any privilege. ------------------------------ Message: 9 Date: Wed, 6 Nov 2013 17:22:14 +0000 From: Joanne Clark Subject: [Histonet] Biopsy Problems To: "histonet@lists.utsouthwestern.edu" Message-ID: <0494A7D4E8CC254EA2FB81464982E378B4ACACEE@S10MAILD001N3.SH10.lan> Content-Type: text/plain; charset="us-ascii" Hi Fellow Histonetters, we have started having this problem with our G.I. biopsies, and not all of them, just the odd one here and there. After staining, when looking at it on the scope, you can't get it to focus in one plane. It's not cutting artifact, because we have recut the block making sure it is well cooled etc. before picking up and staining the section. The cells, when in focus, look fixed and properly processed, and the staining is the way it should be. I have no idea what is causing this artifact. Has anyone else seen this and can shed some light on what is causing it? Joanne Clark, HT Director of Histology Pathology Consultants of New Mexico Disclaimer: This electronic message may contain information that is proprietary, confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 120, Issue 7 **************************************** From Joyce.Weems <@t> emoryhealthcare.org Wed Nov 6 12:15:16 2013 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Wed Nov 6 12:15:28 2013 Subject: [Histonet] RE: unsubscribe In-Reply-To: References: Message-ID: Don't fuss at her - I told her what to do!! :>) Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of McCarty, Jean [JRDUS] Sent: Wednesday, November 06, 2013 1:05 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] unsubscribe -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Wednesday, November 06, 2013 1:02 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 120, Issue 7 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. FW: Fungus Controls (Tim Higgins) 2. RE: Histonet Digest, Vol 120, Issue 6 (Gray, Ed) 3. RE: FDA Disclaimer (Hannen, Valerie) (Jean Wood) 4. RE: FW: Fungus Controls (Tom McNemar) 5. CD 34 antibody abcam (ab963) (tahseen) 6. Water rinse for H&E's (Pardue, Judith) 7. Re: Water rinse for H&E's (Rene J Buesa) 8. RE: Water rinse for H&E's (Terri Brown) 9. Biopsy Problems (Joanne Clark) ---------------------------------------------------------------------- Message: 1 Date: Tue, 5 Nov 2013 13:03:54 -0600 From: Tim Higgins Subject: [Histonet] FW: Fungus Controls To: "histonet@lists.utsouthwestern.edu" , "Vickroy.Jim@mhsil.com" Message-ID: Content-Type: text/plain; charset="iso-8859-1" I have used orange peel for years. I never had a problem with the pathologist not approving the control for use on patient stains. Its "naturally occurring", a petri dish would not be naturally occurring in my mind. Once I verified the orange peel does stain the fungus the same way it does in human tissue by doing a side by side staining the pathologist were fine with it. Actually happy I was able to save some money. Most controls you get from other source are not human either, usually it some type of animal. Try the orange peel. I did like the idea of using the some type of moldy meat or having your micro department do something for you!!!! I have never tried that, but if it works that would be awesome. Thanks, Timothy N. Higgins, HT (ASCP), QIHC > Message: 4 > Date: Mon, 4 Nov 2013 15:07:06 -0500 > From: Patrick Laurie > Subject: Re: [Histonet] Aspergillus tissue blocks for controls > To: "Vickroy, Jim" > Cc: "histonet@lists.utsouthwestern.edu" > > Message-ID: > > Content-Type: text/plain; charset=ISO-8859-1 > > Hi Jim, > > I have found a couple of ways. First, if you are fortunate to have a micro > department nearby, they can make a very nice one for you (a Histotip in > Sakura's Histologic). Another method, which doesn't work the best, can be > moldy orange peel. And the easiest, you can create your own (moldy > sausage) or even take some human tissue (fresh lung works the best) and > leave it with some moldy meat, then you can get a great one. > > Good luck! > > > On Mon, Nov 4, 2013 at 10:38 AM, Vickroy, Jim wrote: > > > We are in desperate needs of obtaining GMS controls. In the old days we > > had all the fungal specimens we needed. Today it is getting hard to find > > these tissues or controls. Does anyone have any idea where we can get > > tissue blocks with Aspergillus or does anyone have any other suggestions > > about GMS controls. > > > > James Vickroy BS, HT(ASCP) > > > > Surgical and Autopsy Pathology Technical Supervisor > > Memorial Medical Center > > 217-788-4046 > > > > ------------------------------ Message: 2 Date: Tue, 5 Nov 2013 19:20:42 +0000 From: "Gray, Ed" Subject: [Histonet] RE: Histonet Digest, Vol 120, Issue 6 To: "histonet@lists.utsouthwestern.edu" Message-ID: <6a889df9e216449bbac73ab5d795aa71@BN1PR05MB406.namprd05.prod.outlook.com> Content-Type: text/plain; charset="us-ascii" You actually need the performing lab's disclaimer. We've sent stains and other procedures to several other labs. We refer to the disclaimers as ASR's (analyte specific reagents) and build templates for each lab's specific comments in our APLIS. Ed Gray | Pathology IT Analyst | Phone: 304-293-2945 | Fax 304-293-1627 | WVU Healthcare l egray@wvuhealthcare.com Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. Message: 9 Date: Tue, 5 Nov 2013 05:47:53 -0500 From: "Hannen, Valerie" Subject: [Histonet] FDA Disclaimer To: "Histonet Post (histonet@lists.utsouthwestern.edu)" Message-ID: <450B7A81EDA0C54E97C53D60F00776C3232B4BB696@isexstore03> Content-Type: text/plain; charset="us-ascii" Good Morning... I have a question about the FDA disclaimer for Immuno's. If we are not doing the staining of Immuno's in our lab, but our Pathologists are interpreting those that are stained at our reference lab, are we still required to put the FDA disclaimer on our Path reports for those antibodies that require the disclaimer? Thanks so much!! Valerie Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.hannen@parrishmed.com =================== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" =================== ------------------------------ Message: 3 Date: Tue, 5 Nov 2013 13:41:14 -0800 From: Jean Wood Subject: [Histonet] RE: FDA Disclaimer (Hannen, Valerie) To: "histonet@lists.utsouthwestern.edu" Message-ID: <87A4EF3F1FBA9D42A7D1EC82F031C0B80A4FD115D6@cliff> Content-Type: text/plain; charset="us-ascii" Valerie, If your pathologist is reading your IHC send-outs then he is most likely contracted through that third party company (he gets PC compensation) and they do the technicial work. In our case, our pathologist signs out our routine H&E Final Surgical Pathology Report and when he reads the IHC he uses their report portal to issue those results using their letterhead with the FDA disclaimer attached at the end of the report. Sometimes he may incorporate the IHC results in the original report as revised but we always piggy back the third party IHC report (with digital pathology images) to the original. It might be best to contact the IHC company representative and find out excatly who is legally responsible for the disclaimer - the technical service provider or the proffesional. Jean Wood BS, HT Fairchild Medical Center Pathology Dept. Ph:530.841.6243 Fax:530.841.6232 jwood@fairchildmed.org ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu [histonet-request@lists.utsouthwestern.edu] Sent: Tuesday, November 05, 2013 10:05 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 120, Issue 6 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: basement membranes (Lee & Peggy Wenk) 2. (no subject) (Davis, Cassie) 3. GMS fungus controls (Davis, Cassie) 4. Re: Aspergillus tissue blocks for controls (Patrick Laurie) 5. Problems with H&E Bleeding (P.E. Visser) 6. AUTO: Bruce Palmatier is out of the office (returning 11/08/2013) (Bruce_Palmatier@vwr.com) 7. Re: Aspergillus tissue blocks for controls (Hans B Snyder) 8. RE: Aspergillus tissue blocks for controls (Diana McCaig) 9. FDA Disclaimer (Hannen, Valerie) 10. FW: The results of your email commands (Scott, Allison D) 11. Hot Histology Career Opportunities Alert from Pam Barker at RELIA Solutions!! Even if you are happy where you are take a look please we offer a 500.00 referral fee and it's almost time for Christmas shopping!!! 11/5/2013 (Pam Barker) 12. (no subject) (Richard Yeo) 13. Faxitron Xray digital imaging (Scott, Allison D) 14. RE: Faxitron Xray digital imaging (WILLIAM DESALVO) ---------------------------------------------------------------------- Message: 1 Date: Mon, 4 Nov 2013 13:38:25 -0500 From: "Lee & Peggy Wenk" Subject: Re: [Histonet] basement membranes To: "Edwards, Richard E." , Message-ID: <3BC14F9010C4499A88E5351B42787F0B@HP2010> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original Electron Microscope. Even if you did a periodic acid-methenamine silver stain (PASM, Jones), which in my opinion is the best histology stain, since it is a silver stain, you can get different thicknesses of basement membrane (bm) by leaving it in longer or shorter than is optimal for that patient's bm thickness. Peggy A. Wenk, HTL(ASCP)SLS -----Original Message----- From: Edwards, Richard E. Sent: Monday, November 04, 2013 9:39 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] basement membranes Best technique, tinctorial or otherwise of identifying, with a view to measuring their width, many thanks. Richard Edwards Leicester University U.K. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Mon, 4 Nov 2013 13:45:26 -0500 From: "Davis, Cassie" Subject: [Histonet] (no subject) To: "Vickroy.Jim@mhsil.com" Cc: "histonet@lists.utsouthwestern.edu" Message-ID: <08861B9CF6C7774E874635A4818AE37B01C9AC44F5@CHEXCMS01.one.ads.che.org> Content-Type: text/plain; charset="iso-8859-1" Jim, We get our Fungus, GMS Aspergillus controls (slides) and many of our hard to find control slides from Newcomer Supply 1-800-383-7799 or 608-831-7888 Cassandra Davis CDavis@che-east.org 302-575-8095 Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ------------------------------ Message: 3 Date: Mon, 4 Nov 2013 13:46:23 -0500 From: "Davis, Cassie" Subject: [Histonet] GMS fungus controls To: "Vickroy.Jim@mhsil.com" Cc: "histonet@lists.utsouthwestern.edu" Message-ID: <08861B9CF6C7774E874635A4818AE37B01C9AC44F9@CHEXCMS01.one.ads.che.org> Content-Type: text/plain; charset="iso-8859-1" Jim, We get our Fungus, GMS Aspergillus controls (slides) and many of our hard to find control slides from Newcomer Supply 1-800-383-7799 or 608-831-7888 Cassandra Davis CDavis@che-east.org 302-575-8095 Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ------------------------------ Message: 4 Date: Mon, 4 Nov 2013 15:07:06 -0500 From: Patrick Laurie Subject: Re: [Histonet] Aspergillus tissue blocks for controls To: "Vickroy, Jim" Cc: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hi Jim, I have found a couple of ways. First, if you are fortunate to have a micro department nearby, they can make a very nice one for you (a Histotip in Sakura's Histologic). Another method, which doesn't work the best, can be moldy orange peel. And the easiest, you can create your own (moldy sausage) or even take some human tissue (fresh lung works the best) and leave it with some moldy meat, then you can get a great one. Good luck! On Mon, Nov 4, 2013 at 10:38 AM, Vickroy, Jim wrote: > We are in desperate needs of obtaining GMS controls. In the old days we > had all the fungal specimens we needed. Today it is getting hard to find > these tissues or controls. Does anyone have any idea where we can get > tissue blocks with Aspergillus or does anyone have any other suggestions > about GMS controls. > > James Vickroy BS, HT(ASCP) > > Surgical and Autopsy Pathology Technical Supervisor > Memorial Medical Center > 217-788-4046 > > > ________________________________ > This message (including any attachments) contains confidential information > intended for a specific individual and purpose, and is protected by law. If > you are not the intended recipient, you should delete this message. Any > disclosure, copying, or distribution of this message, or the taking of any > action based on it, is strictly prohibited. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie(HT)ASCP QIHC Histology Manager Celligent Diagnostics, LLC 101 East W.T. Harris Blvd | Suite 1212 | Charlotte, NC 28262 Work: 704-970-3300 Cell: 704-266-0869 ------------------------------ Message: 5 Date: Mon, 4 Nov 2013 21:28:33 +0100 From: "P.E. Visser" Subject: [Histonet] Problems with H&E Bleeding To: Message-ID: <002301ced99c$7098c9f0$51ca5dd0$@xs4all.nl> Content-Type: text/plain; charset="us-ascii" Dear anna I had while working for Baker chemicals, a customer in Germany who had a similar problem there H&E turned purple. the red disappeared. the environment around the section contained little red droplets (only visible under high magnification). after some time (because at first they clamed noting was changed) it became clear that they skipped ethanol 100 % and just used the cheaper 96% instead. when evaluated they tested 3 slides and that worked. but after 300 - 500 slides the xylene was saturated with water. eosin being soluble in water migrates from the section to the water emulate in the xylene. so I would test the ethanol you use. 3 times 100% is in my view a must. I also on time in Scotland was confronted with xylene that was sold as non recycled, but in fact it was. and sadly the supplier used an open container to speed up cooling down. the rain was the source of the water. it lead to several problems and took a long time before the we could find the source of these problems. wish you success with solving yours. regards piet ------------------------------ Message: 6 Date: Mon, 4 Nov 2013 16:00:59 -0500 From: Bruce_Palmatier@vwr.com Subject: [Histonet] AUTO: Bruce Palmatier is out of the office (returning 11/08/2013) To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII I am out of the office until 11/08/2013. I will be out of the office from Nov 4- Nov 8. If you have a question about an order or need assistance placing an order, or if the matter is urgent, please call 877-881-1192 between 8am and 8pm and a VWR Healthcare Service associate will assist you. For other matters, I will respond to emails within 24 hours. If you require pricing information, quotes, product information, or other assistance, Tim Rafferty is the new VWR Healthcare Sales Rep who's taken my place. He can be reached at timothy_rafferty@vwr.com or (717) 668-9045. Thank You, Bruce Palmatier Market Portfolio Manager VWR Healthcare bruce_palmatier@vwr.com mobile: 484.319.5563 fax: 484-881-7307 Customer Service: 877.881.1192 Fax: 484.881.6486 Customer Service email: HEALTHCARESERVICE@VWR.COM Note: This is an automated response to your message "Histonet Digest, Vol 120, Issue 5" sent on 11/4/2013 1:06:11 PM. This is the only notification you will receive while this person is away. ------------------------------ Message: 7 Date: Mon, 4 Nov 2013 16:46:37 -0500 From: Hans B Snyder Subject: Re: [Histonet] Aspergillus tissue blocks for controls To: Patrick Laurie Cc: "histonet@lists.utsouthwestern.edu" , "Vickroy, Jim" Message-ID: Content-Type: text/plain; charset=ISO-8859-1 We have tried the moldy peel also but the pathologists did not like this and would not accept any controls of this form. I don't blame them they want to see it naturally occurring. Hans B Snyder Histologistics 60 Prescott Street Worcester, MA 01605 508-308-7800 hans@histologistics.com On Mon, Nov 4, 2013 at 3:07 PM, Patrick Laurie wrote: > Hi Jim, > > I have found a couple of ways. First, if you are fortunate to have a micro > department nearby, they can make a very nice one for you (a Histotip in > Sakura's Histologic). Another method, which doesn't work the best, can be > moldy orange peel. And the easiest, you can create your own (moldy > sausage) or even take some human tissue (fresh lung works the best) and > leave it with some moldy meat, then you can get a great one. > > Good luck! > > > On Mon, Nov 4, 2013 at 10:38 AM, Vickroy, Jim > wrote: > > > We are in desperate needs of obtaining GMS controls. In the old days we > > had all the fungal specimens we needed. Today it is getting hard to find > > these tissues or controls. Does anyone have any idea where we can get > > tissue blocks with Aspergillus or does anyone have any other suggestions > > about GMS controls. > > > > James Vickroy BS, HT(ASCP) > > > > Surgical and Autopsy Pathology Technical Supervisor > > Memorial Medical Center > > 217-788-4046 > > > > > > ________________________________ > > This message (including any attachments) contains confidential > information > > intended for a specific individual and purpose, and is protected by law. > If > > you are not the intended recipient, you should delete this message. Any > > disclosure, copying, or distribution of this message, or the taking of > any > > action based on it, is strictly prohibited. > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > -- > > Patrick Laurie(HT)ASCP QIHC > > Histology Manager > > Celligent Diagnostics, LLC > > 101 East W.T. Harris Blvd | Suite 1212 | Charlotte, NC 28262 > > Work: 704-970-3300 Cell: 704-266-0869 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 8 Date: Mon, 4 Nov 2013 22:31:18 +0000 From: Diana McCaig Subject: RE: [Histonet] Aspergillus tissue blocks for controls To: "'Hans B Snyder'" , Patrick Laurie Cc: "histonet@lists.utsouthwestern.edu" , "Vickroy, Jim" Message-ID: Content-Type: text/plain; charset="us-ascii" As well we have used mushroom and other moldy food products but it only demonstrates the mold and not tissue elements. But you can mince tissue and mix it with the fungus or mold so it one big segment and this gave more desirable results. Diana -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hans B Snyder Sent: November-04-13 4:47 PM To: Patrick Laurie Cc: histonet@lists.utsouthwestern.edu; Vickroy, Jim Subject: Re: [Histonet] Aspergillus tissue blocks for controls We have tried the moldy peel also but the pathologists did not like this and would not accept any controls of this form. I don't blame them they want to see it naturally occurring. Hans B Snyder Histologistics 60 Prescott Street Worcester, MA 01605 508-308-7800 hans@histologistics.com On Mon, Nov 4, 2013 at 3:07 PM, Patrick Laurie wrote: > Hi Jim, > > I have found a couple of ways. First, if you are fortunate to have a > micro department nearby, they can make a very nice one for you (a > Histotip in Sakura's Histologic). Another method, which doesn't work > the best, can be moldy orange peel. And the easiest, you can create > your own (moldy > sausage) or even take some human tissue (fresh lung works the best) > and leave it with some moldy meat, then you can get a great one. > > Good luck! > > > On Mon, Nov 4, 2013 at 10:38 AM, Vickroy, Jim > wrote: > > > We are in desperate needs of obtaining GMS controls. In the old days we > > had all the fungal specimens we needed. Today it is getting hard to find > > these tissues or controls. Does anyone have any idea where we can get > > tissue blocks with Aspergillus or does anyone have any other > > suggestions about GMS controls. > > > > James Vickroy BS, HT(ASCP) > > > > Surgical and Autopsy Pathology Technical Supervisor Memorial > > Medical Center > > 217-788-4046 > > > > > > ________________________________ > > This message (including any attachments) contains confidential > information > > intended for a specific individual and purpose, and is protected by law. > If > > you are not the intended recipient, you should delete this message. > > Any disclosure, copying, or distribution of this message, or the > > taking of > any > > action based on it, is strictly prohibited. > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > -- > > Patrick Laurie(HT)ASCP QIHC > > Histology Manager > > Celligent Diagnostics, LLC > > 101 East W.T. Harris Blvd | Suite 1212 | Charlotte, NC 28262 > > Work: 704-970-3300 Cell: 704-266-0869 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Tue, 5 Nov 2013 05:47:53 -0500 From: "Hannen, Valerie" Subject: [Histonet] FDA Disclaimer To: "Histonet Post (histonet@lists.utsouthwestern.edu)" Message-ID: <450B7A81EDA0C54E97C53D60F00776C3232B4BB696@isexstore03> Content-Type: text/plain; charset="us-ascii" Good Morning... I have a question about the FDA disclaimer for Immuno's. If we are not doing the staining of Immuno's in our lab, but our Pathologists are interpreting those that are stained at our reference lab, are we still required to put the FDA disclaimer on our Path reports for those antibodies that require the disclaimer? Thanks so much!! Valerie Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.hannen@parrishmed.com =================== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" =================== ------------------------------ Message: 10 Date: Tue, 5 Nov 2013 14:50:15 +0000 From: "Scott, Allison D" Subject: [Histonet] FW: The results of your email commands To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Sent: Tuesday, November 05, 2013 8:48 AM To: Scott, Allison D Subject: The results of your email commands The results of your email command are provided below. Attached is your original message. - Results: Ignoring non-text/plain MIME parts - Unprocessed: Sent: Tuesday, November 05, 2013 8:47 AM To: histonet-request@lists.utsouthwestern.edu Subject: FW: FaxitronX-Ray digital imaging From: Scott, Allison D Sent: Tuesday, November 05, 2013 8:46 AM To: histonet-request@lists.utsouthwestern.edu Subject: Faxitron/X-Ray digital imaging Hello to all in histoland. Our chief pathologist is interested in getting = a faxitron xray digital imaging machine. Is anyone out there using this te= chnology and can you suggest a good company in which to order one from. An= y help in this will be greatly appreciated. Allison Scott HT(ASCP) Supervisor, Histology Lab LBJ Hospital Harris Health System - Ignored: Office: 713-566-2148 Lab: 713-566-5287 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from = your computer system. To the extent the information in this e-mail and any attachments contain = protected health information as defined by the Health Insurance Portability = and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and = 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/o= r = privileged. This e-mail may also be confidential and/or privileged under = Texas law. The e-mail is for the use of only the individual or entity name= d = above. If you are not the intended recipient, or any authorized = representative of the intended recipient, you are hereby notified that any = review, dissemination or copying of this e-mail and its attachments is = strictly prohibited. - Done. CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. ------------------------------ Message: 11 Date: Tue, 5 Nov 2013 10:08:46 -0500 From: "Pam Barker" Subject: [Histonet] Hot Histology Career Opportunities Alert from Pam Barker at RELIA Solutions!! Even if you are happy where you are take a look please we offer a 500.00 referral fee and it's almost time for Christmas shopping!!! 11/5/2013 To: "Histonet" Message-ID: <00dc01ceda38$ecf3afa0$c6db0ee0$@earthlink.net> Content-Type: text/plain; charset="us-ascii" Hi Histonetters!! I hope you are off to the start of a great week on this beautiful Fall Day. I wanted to drop you a line about some of the opportunities that I am currently working on. These clients aren't just "kicking tires" they are ready to interview and ready to hire. If you are looking to make a change now or make a commitment now and the actual change after the holidays if you are the right person for my client they will do what it takes to get you I guarantee it!! So grab a cup of coffee or a mug of tea and something pumpkin flavored (isn't everything pumpkin flavored this time of year?) and please take a minute to peruse my list of current openings. All of the positions I work with are fulltime permanent positions with some of the best facilities Nationwide. My clients offer excellent compensation including competitive salaries, great benefits, relocation assistance/sign on bonuses. Here is a list of my current openings: HISTOLOGY SUPERVISORS/MANAGERS: WA OH MA GA Pathology Supervisor - Seattle, WA Lead Pathology Tech - East of Columbus, OH Histology Supervisor - Boston, MA Histology Supervisor - Atlanta, GA HISTOTECHNICIANS/HISTOTECHNOLOGISTS: VA OH NM MA Histology Tech - Roanoke, VA Histotechnician Day shift -East of Columbus, OH Grossing Histotechnician Dayshift - Las Cruces, NM Histotechnician - Nights - Nashville, TN Night Shift Grossing Histotech - Boston, MA If you or any of your friends would like more information on any of these positions or if you would like to discuss opportunities in other areas or future job searches please contact me. I would be more than happy to assist you. You can reach me at 866-607-3542 or relia1@earthlink.net Remember I offer over 20 years of recruiting and employment counseling experience, knowledgeable, confidential and responsive service to you and your friends and a permanent placement practice dedicated to the histology profession. I work with facilities nationwide and I will keep your resume confidential. I will only represent you to jobs you tell me you are interested in looking into. Remember. It never hurts to keep an eye open even if you are happy in your present job. Pam - 866-607-3542 (866-60RELIA) Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia ------------------------------ Message: 12 Date: Tue, 5 Nov 2013 10:17:26 -0500 From: "Richard Yeo" Subject: [Histonet] (no subject) To: Message-ID: <17E893BEFAF44C0EAD05AF8876EDAF80.MAI@foxmail.wchosp.org> Content-Type: text/plain; charset="utf-8" I have an abundance of fungus controls. I need afb and h.pylori. I would be me know an Thanks Richard Yeo ryeo@foxma ------------------------------ Message: 13 Date: Tue, 5 Nov 2013 16:00:11 +0000 From: "Scott, Allison D" Subject: [Histonet] Faxitron Xray digital imaging To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Hello to all in histoland. Our chief pathologist is interested in getting a faxitron xray digital imaging machine. Is anyone out there using this technology and can you suggest a company in which to order one from. Any help in this will be greatly appreciated. Allison Scott HT(ASCP) Supervisor, Histology Lab LBJ Hospital Harris Health System Office: 713-566-2148 Lab: 713-566-5287 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. ------------------------------ Message: 14 Date: Tue, 5 Nov 2013 09:47:16 -0700 From: WILLIAM DESALVO Subject: RE: [Histonet] Faxitron Xray digital imaging To: "Scott, Allison D" , histonet Message-ID: Content-Type: text/plain; charset="iso-8859-1" We have used the Faxitron instrument for the past 3 years at multiple sites. Faxitron creates a digital image of a specimen quickly and safely and a t multiple magnifications. The instrument is placed in either Surgery or Surgical Pathology (SP) and we use it primarily on breast cases to assist in orientation and gross dissection, identifying radioactive seeds or locating micro calcification. The instrument is easy to use, safe and you can connect to multiple LIS systems and a Hospital PACS. There is no code to charge for use when used in SP, only when used by Radiology or Surgery. There is only one company that manufactures and sells the instrument: Faxitron Bioptics, LLC Tucson, AZ 877-910-0030 The are willing to bring a unit to your site for demonstration and you may be able to talk them into leaving for a evaluation. William DeSalvo, BS HTL(ASCP) > From: Allison.Scott@harrishealth.org > To: histonet@lists.utsouthwestern.edu > Date: Tue, 5 Nov 2013 16:00:11 +0000 > Subject: [Histonet] Faxitron Xray digital imaging > > Hello to all in histoland. Our chief pathologist is interested in getting a faxitron xray digital imaging machine. Is anyone out there using this technology and can you suggest a company in which to order one from. Any help in this will be greatly appreciated. > > > > > Allison Scott HT(ASCP) > Supervisor, Histology Lab > LBJ Hospital > Harris Health System > Office: 713-566-2148 > Lab: 713-566-5287 > > > CONFIDENTIALITY NOTICE: > If you have received this e-mail in error, please immediately notify the > sender by return e-mail and delete this e-mail and any attachments from > your computer system. > > To the extent the information in this e-mail and any attachments contain > protected health information as defined by the Health Insurance Portability > and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and > 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or > privileged. This e-mail may also be confidential and/or privileged under > Texas law. The e-mail is for the use of only the individual or entity named > above. If you are not the intended recipient, or any authorized > representative of the intended recipient, you are hereby notified that any > review, dissemination or copying of this e-mail and its attachments is > strictly prohibited. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 120, Issue 6 **************************************** ------------------------------ Message: 4 Date: Wed, 6 Nov 2013 05:37:13 -0500 From: Tom McNemar Subject: RE: [Histonet] FW: Fungus Controls To: 'Tim Higgins' , "histonet@lists.utsouthwestern.edu" , "Vickroy.Jim@mhsil.com" Message-ID: Content-Type: text/plain; charset="us-ascii" I made some controls years ago that we are still using today. I used bread mold and streaked it out on a blood agar plate. Once the plate was covered, cut the agar into cubes, wrapped it in lens paper and processed. Cuts and stains beautifully. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tim Higgins Sent: Tuesday, November 05, 2013 2:04 PM To: histonet@lists.utsouthwestern.edu; Vickroy.Jim@mhsil.com Subject: [Histonet] FW: Fungus Controls I have used orange peel for years. I never had a problem with the pathologist not approving the control for use on patient stains. Its "naturally occurring", a petri dish would not be naturally occurring in my mind. Once I verified the orange peel does stain the fungus the same way it does in human tissue by doing a side by side staining the pathologist were fine with it. Actually happy I was able to save some money. Most controls you get from other source are not human either, usually it some type of animal. Try the orange peel. I did like the idea of using the some type of moldy meat or having your micro department do something for you!!!! I have never tried that, but if it works that would be awesome. Thanks, Timothy N. Higgins, HT (ASCP), QIHC > Message: 4 > Date: Mon, 4 Nov 2013 15:07:06 -0500 > From: Patrick Laurie > Subject: Re: [Histonet] Aspergillus tissue blocks for controls > To: "Vickroy, Jim" > Cc: "histonet@lists.utsouthwestern.edu" > > Message-ID: > > Content-Type: text/plain; charset=ISO-8859-1 > > Hi Jim, > > I have found a couple of ways. First, if you are fortunate to have a micro > department nearby, they can make a very nice one for you (a Histotip in > Sakura's Histologic). Another method, which doesn't work the best, can be > moldy orange peel. And the easiest, you can create your own (moldy > sausage) or even take some human tissue (fresh lung works the best) and > leave it with some moldy meat, then you can get a great one. > > Good luck! > > > On Mon, Nov 4, 2013 at 10:38 AM, Vickroy, Jim wrote: > > > We are in desperate needs of obtaining GMS controls. In the old days we > > had all the fungal specimens we needed. Today it is getting hard to find > > these tissues or controls. Does anyone have any idea where we can get > > tissue blocks with Aspergillus or does anyone have any other suggestions > > about GMS controls. > > > > James Vickroy BS, HT(ASCP) > > > > Surgical and Autopsy Pathology Technical Supervisor > > Memorial Medical Center > > 217-788-4046 > > > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ------------------------------ Message: 5 Date: Wed, 06 Nov 2013 17:33:59 +0500 From: tahseen Subject: [Histonet] CD 34 antibody abcam (ab963) To: Message-ID: Content-Type: text/plain; charset=UTF-8; format=flowed Hi All, We used CD 34 antibody abcam (ab963)with detection kit enVision to stain our samples from rats and rabbits. We need to stain vessels only but macrophages, fibroblast, collagen all are stained in other hand known +ve control (Tonsil) is perform good. Please help us that what should we do for IHC staining of our samples. Best regards Muhammad Tahseen Sr.Supervisor Histology SKMCH&RC Lahore ------------------------------ Message: 6 Date: Wed, 6 Nov 2013 15:38:53 +0000 From: "Pardue, Judith" Subject: [Histonet] Water rinse for H&E's To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Does anyone use hot water rinses on their h&e slides. If we use cold water our slides are very light and inconsistent. We stain on an automatic stainer. Judith Pardue Histology Supervisor Memorial Health Care System judith_pardue@memorial.org This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. ------------------------------ Message: 7 Date: Wed, 6 Nov 2013 07:50:32 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] Water rinse for H&E's To: "Pardue, Judith" , "histonet@lists.utsouthwestern.edu" Message-ID: <1383753032.32943.YahooMailNeo@web163103.mail.bf1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I do not, but it seems that you need to use hot water. If it works for you, keep doing it. ? The rationale behind this issue is that water after the staining is not just waterto wash the sections, but also to change the pH of the hematoxylin so the stain is, lets say, "developed" and this is a process temperature driven (the speed of the reaction is directly proportional to the temp.) and if you are having weak results with your running water (evidently cold) and good with hot water, this demonstrates that you need hot water in your specific circumstances. I hope I explained myself Ren? J. ________________________________ From: "Pardue, Judith" To: "histonet@lists.utsouthwestern.edu" Sent: Wednesday, November 6, 2013 10:38 AM Subject: [Histonet] Water rinse for H&E's Does anyone use hot water rinses on their h&e slides. If we use cold water our slides are very light and inconsistent. We stain on an automatic stainer. Judith Pardue Histology Supervisor Memorial Health Care System judith_pardue@memorial.org This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Wed, 6 Nov 2013 12:20:48 -0500 From: "Terri Brown" Subject: RE: [Histonet] Water rinse for H&E's To: "Pardue, Judith" , Message-ID: <731941C266951A47BEF11E5EFAAED9C91EF5D2F4@nsmvexch01.northside.local> Content-Type: text/plain;charset="us-ascii" We use warm water rinses for our H&E slides on our automatic stainers. Terri H. Brown,, HT (ASCP) Pathology Laboratory Manager Northside Hospital Atlanta terri.brown@northside.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pardue, Judith Sent: Wednesday, November 06, 2013 10:39 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Water rinse for H&E's Does anyone use hot water rinses on their h&e slides. If we use cold water our slides are very light and inconsistent. We stain on an automatic stainer. Judith Pardue Histology Supervisor Memorial Health Care System judith_pardue@memorial.org This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by Northside Hospital. It may contain information that is confidential, privileged, proprietary, or otherwise legally exempt from disclosure. If you are not the intended recipient, you are hereby notified that you are not authorized to read, print, retain, copy or disseminate this message, any part of it, or any attachments. If you have received this message in error, please delete this message and any attachments from your system without reading the content and notify the sender immediately of the inadvertent transmission. There is no intent on the part of the sender to waive any privilege. ------------------------------ Message: 9 Date: Wed, 6 Nov 2013 17:22:14 +0000 From: Joanne Clark Subject: [Histonet] Biopsy Problems To: "histonet@lists.utsouthwestern.edu" Message-ID: <0494A7D4E8CC254EA2FB81464982E378B4ACACEE@S10MAILD001N3.SH10.lan> Content-Type: text/plain; charset="us-ascii" Hi Fellow Histonetters, we have started having this problem with our G.I. biopsies, and not all of them, just the odd one here and there. After staining, when looking at it on the scope, you can't get it to focus in one plane. It's not cutting artifact, because we have recut the block making sure it is well cooled etc. before picking up and staining the section. The cells, when in focus, look fixed and properly processed, and the staining is the way it should be. I have no idea what is causing this artifact. Has anyone else seen this and can shed some light on what is causing it? Joanne Clark, HT Director of Histology Pathology Consultants of New Mexico Disclaimer: This electronic message may contain information that is proprietary, confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 120, Issue 7 **************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From Sherrian.McAnn <@t> va.gov Wed Nov 6 12:17:15 2013 From: Sherrian.McAnn <@t> va.gov (McAnn, Sherrian) Date: Wed Nov 6 12:18:06 2013 Subject: [Histonet] Biopsy Problems Message-ID: <61E2B58CECEF384094A363989D47C0900A61CB45@VHAV17MSGA2.v17.med.va.gov> Seems as though they may be lifting off in spots? Or maybe perhaps vibration? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joanne Clark Sent: Wednesday, November 06, 2013 11:22 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Biopsy Problems Hi Fellow Histonetters, we have started having this problem with our G.I. biopsies, and not all of them, just the odd one here and there. After staining, when looking at it on the scope, you can't get it to focus in one plane. It's not cutting artifact, because we have recut the block making sure it is well cooled etc. before picking up and staining the section. The cells, when in focus, look fixed and properly processed, and the staining is the way it should be. I have no idea what is causing this artifact. Has anyone else seen this and can shed some light on what is causing it? Joanne Clark, HT Director of Histology Pathology Consultants of New Mexico Disclaimer: This electronic message may contain information that is proprietary, confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> gmail.com Wed Nov 6 12:38:31 2013 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Wed Nov 6 12:38:35 2013 Subject: [Histonet] Re: Fungus controls Message-ID: I wouldn't be too happy with any of these substitutes for proper fungus controls. Ideally, your control would be the fungus you're looking for, obviously not a practical way to do it. The usual fungus control I see is mouse lung with yeast, presumably Candida, injected into normal lung tissue. This control will stain very satisfactorily with PAS, and is not really a good test of the efficacy of a fungus stain. The most exacting fungus control for the GMS stain is histoplasma, an old infection in which some of the yeast cells are actually dead. If you can stain that, you can stain any fungus you're likely to encounter. Freida Carson did an elegant study a number of years ago (reference below) of how critical the oxidation step is in this endeavor. The decline of medical autopsies has made controls like this much more difficult to obtain, but when you can get it, you can get large quantities of it which need to be effectively shared. Bob Richmond Samurai Pathologist Maryville TN *************************** Inconsistent Detection of Histoplasma capsulatum with Periodic Acid Oxidation in the Grocott Methenamine-Silver Nitrate (GMS) Fungus Stain Freida L. Carson, Jerry Fredenburgh, and John E. Maxwell 1. Department of Pathology, Baylor University Medical Center 2. Richard-Allan Scientific Kalamazoo MI 3. Glenwood Regional Medical Center, West Monroe LA J Histotechnol [June] 1999;22:119 From joelleweaver <@t> hotmail.com Wed Nov 6 14:25:39 2013 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Wed Nov 6 14:25:46 2013 Subject: [Histonet] On call hours required of staff Message-ID: Sorry if this re-posted- it got bounced back to me once. Please advise of the number of hours that your histology staff are scheduled to take on-call in addition to their 40 scheduled hours ( FT staff/hourly) ? If you have a maximum # of on call hours by your lab or HR policies, that would be most helpful. The proposal is to begin a usual 40 worked hours scheduled in the lab and then ~ 15 hours overnight on call for each weekday,( M-F), and potentially 8 hours or more each weekend day. I think this proposal is unreasonable, and 75 hours + 16 hours potential on call/week ( 91 hours on call?). I would appreciate your thoughts , comments? I hope to derail this with something more reasonable Joelle Weaver MAOM, HTL (ASCP) QIHC From mpence <@t> grhs.net Wed Nov 6 14:34:17 2013 From: mpence <@t> grhs.net (Mike Pence) Date: Wed Nov 6 14:34:41 2013 Subject: [Histonet] On call hours required of staff In-Reply-To: References: Message-ID: Will the techs be paid call pay? What is being asked of the techs to do on call? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelle weaver Sent: Wednesday, November 06, 2013 2:26 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] On call hours required of staff Sorry if this re-posted- it got bounced back to me once. Please advise of the number of hours that your histology staff are scheduled to take on-call in addition to their 40 scheduled hours ( FT staff/hourly) ? If you have a maximum # of on call hours by your lab or HR policies, that would be most helpful. The proposal is to begin a usual 40 worked hours scheduled in the lab and then ~ 15 hours overnight on call for each weekday,( M-F), and potentially 8 hours or more each weekend day. I think this proposal is unreasonable, and 75 hours + 16 hours potential on call/week ( 91 hours on call?). I would appreciate your thoughts , comments? I hope to derail this with something more reasonable Joelle Weaver MAOM, HTL (ASCP) QIHC _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Wed Nov 6 14:45:54 2013 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Wed Nov 6 14:45:58 2013 Subject: [Histonet] On call hours required of staff In-Reply-To: References: , Message-ID: No pay has been mentioned. The call hours are to be covered initially by one person . The tasks are potentially take care of anything occurring during those hours including stat specimens. The primary concern of management is that a refrigerator or the room temperature or humidity will go out of range when staff are not on site. There is a 24 hour monitor for environmental stuff that will call to the person's cell phone, and anything else that occurs when no one is scheduled in the area will result in a cell call. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: mpence@grhs.net > To: joelleweaver@hotmail.com; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] On call hours required of staff > Date: Wed, 6 Nov 2013 20:34:17 +0000 > > Will the techs be paid call pay? What is being asked of the techs to do on call? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelle weaver > Sent: Wednesday, November 06, 2013 2:26 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] On call hours required of staff > > Sorry if this re-posted- it got bounced back to me once. > > Please advise of the number of hours that your histology staff are scheduled to take on-call in addition to their 40 scheduled hours ( FT staff/hourly) ? > If you have a maximum # of on call hours by your lab or HR policies, that would be most helpful. > The proposal is to begin a usual 40 worked hours scheduled in the lab and then ~ 15 hours overnight on call for each weekday,( M-F), and potentially 8 hours or more each weekend day. I think this proposal is unreasonable, and 75 hours + 16 hours potential on call/week ( 91 hours on call?). > I would appreciate your thoughts , comments? I hope to derail this with something more reasonable > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From HornHV <@t> archildrens.org Wed Nov 6 15:01:29 2013 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Wed Nov 6 15:01:43 2013 Subject: [Histonet] On call hours required of staff In-Reply-To: References: , Message-ID: <25A4DE08332B19499904459F00AAACB719C704FEB6@EVS1.archildrens.org> I would not ask my techs to take call for no pay. We have to respond to call in 1 hour. The tech would not be able to make personal plans if they are on call. We are on call on long weekends. We take turns taking call and are paid call pay. If we get called in, it is overtime. On the rare occasion someone needs to work Saturday, one of us volunteer and are paid call pay and overtime. As a supervisor I am potentially on call all of the time. If our tissue processor alarms, I am called. I come in and I get call pay and overtime. (This is a rare occurrence) If I am unavailable, my senior tech answers calls. I also answer after hours calls from the lab. Usually a phone call takes care of it. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelle weaver Sent: Wednesday, November 06, 2013 2:46 PM To: Mike Pence; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On call hours required of staff No pay has been mentioned. The call hours are to be covered initially by one person . The tasks are potentially take care of anything occurring during those hours including stat specimens. The primary concern of management is that a refrigerator or the room temperature or humidity will go out of range when staff are not on site. There is a 24 hour monitor for environmental stuff that will call to the person's cell phone, and anything else that occurs when no one is scheduled in the area will result in a cell call. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: mpence@grhs.net > To: joelleweaver@hotmail.com; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] On call hours required of staff > Date: Wed, 6 Nov 2013 20:34:17 +0000 > > Will the techs be paid call pay? What is being asked of the techs to do on call? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelle weaver > Sent: Wednesday, November 06, 2013 2:26 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] On call hours required of staff > > Sorry if this re-posted- it got bounced back to me once. > > Please advise of the number of hours that your histology staff are scheduled to take on-call in addition to their 40 scheduled hours ( FT staff/hourly) ? > If you have a maximum # of on call hours by your lab or HR policies, that would be most helpful. > The proposal is to begin a usual 40 worked hours scheduled in the lab and then ~ 15 hours overnight on call for each weekday,( M-F), and potentially 8 hours or more each weekend day. I think this proposal is unreasonable, and 75 hours + 16 hours potential on call/week ( 91 hours on call?). > I would appreciate your thoughts , comments? I hope to derail this with something more reasonable > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From mpence <@t> grhs.net Wed Nov 6 15:08:29 2013 From: mpence <@t> grhs.net (Mike Pence) Date: Wed Nov 6 15:08:57 2013 Subject: [Histonet] On call hours required of staff In-Reply-To: <25A4DE08332B19499904459F00AAACB719C704FEB6@EVS1.archildrens.org> References: , <25A4DE08332B19499904459F00AAACB719C704FEB6@EVS1.archildrens.org> Message-ID: No pay, no call! I don't think you could require your techs to take call without paying them for their on-call time. Maybe you have one or two techs that would not mind the extra money being on call. I remember back several years ago (maybe a million) when I was younger I would have taken anything for more money! -----Original Message----- From: Horn, Hazel V [mailto:HornHV@archildrens.org] Sent: Wednesday, November 06, 2013 3:01 PM To: 'joelle weaver'; Mike Pence; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On call hours required of staff I would not ask my techs to take call for no pay. We have to respond to call in 1 hour. The tech would not be able to make personal plans if they are on call. We are on call on long weekends. We take turns taking call and are paid call pay. If we get called in, it is overtime. On the rare occasion someone needs to work Saturday, one of us volunteer and are paid call pay and overtime. As a supervisor I am potentially on call all of the time. If our tissue processor alarms, I am called. I come in and I get call pay and overtime. (This is a rare occurrence) If I am unavailable, my senior tech answers calls. I also answer after hours calls from the lab. Usually a phone call takes care of it. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelle weaver Sent: Wednesday, November 06, 2013 2:46 PM To: Mike Pence; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On call hours required of staff No pay has been mentioned. The call hours are to be covered initially by one person . The tasks are potentially take care of anything occurring during those hours including stat specimens. The primary concern of management is that a refrigerator or the room temperature or humidity will go out of range when staff are not on site. There is a 24 hour monitor for environmental stuff that will call to the person's cell phone, and anything else that occurs when no one is scheduled in the area will result in a cell call. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: mpence@grhs.net > To: joelleweaver@hotmail.com; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] On call hours required of staff > Date: Wed, 6 Nov 2013 20:34:17 +0000 > > Will the techs be paid call pay? What is being asked of the techs to do on call? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelle > weaver > Sent: Wednesday, November 06, 2013 2:26 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] On call hours required of staff > > Sorry if this re-posted- it got bounced back to me once. > > Please advise of the number of hours that your histology staff are scheduled to take on-call in addition to their 40 scheduled hours ( FT staff/hourly) ? > If you have a maximum # of on call hours by your lab or HR policies, that would be most helpful. > The proposal is to begin a usual 40 worked hours scheduled in the lab and then ~ 15 hours overnight on call for each weekday,( M-F), and potentially 8 hours or more each weekend day. I think this proposal is unreasonable, and 75 hours + 16 hours potential on call/week ( 91 hours on call?). > I would appreciate your thoughts , comments? I hope to derail this > with something more reasonable > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From joelleweaver <@t> hotmail.com Wed Nov 6 15:09:46 2013 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Wed Nov 6 15:09:50 2013 Subject: [Histonet] On call hours required of staff In-Reply-To: <25A4DE08332B19499904459F00AAACB719C704FEB6@EVS1.archildrens.org> References: , , , , <25A4DE08332B19499904459F00AAACB719C704FEB6@EVS1.archildrens.org> Message-ID: Hazel Yes, that sounds more reasonable. I have been on call for many years while on the bench- just never that much, or for that many consecutive hours individually. You always have set hours that you have to be available and respond within certain time frames and the days are rotated for on call. I never minded that much, and you can usually trade with someone if you have something important you need to do that takes you out of town. Of course, I would hope for some compensation for the obliteration of my personal life with that many hours. Appreciate your thoughts on this. If your lab has a policy for this that you can attach or copy to me the highlights in an email this will help me bargain. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: HornHV@archildrens.org > To: joelleweaver@hotmail.com; mpence@grhs.net; histonet@lists.utsouthwestern.edu > Date: Wed, 6 Nov 2013 15:01:29 -0600 > Subject: RE: [Histonet] On call hours required of staff > > I would not ask my techs to take call for no pay. We have to respond to call in 1 hour. The tech would not be able to make personal plans if they are on call. > > We are on call on long weekends. We take turns taking call and are paid call pay. If we get called in, it is overtime. On the rare occasion someone needs to work Saturday, one of us volunteer and are paid call pay and overtime. > > As a supervisor I am potentially on call all of the time. If our tissue processor alarms, I am called. I come in and I get call pay and overtime. (This is a rare occurrence) If I am unavailable, my senior tech answers calls. I also answer after hours calls from the lab. Usually a phone call takes care of it. > > Hazel Horn > Supervisor of Histology/Autopsy/Transcription > Anatomic Pathology > Arkansas Children's Hospital > 1 Children's Way | Slot 820| Little Rock, AR 72202 > 501.364.4240 direct | 501.364.1241 fax > hornhv@archildrens.org > archildrens.org > > > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelle weaver > Sent: Wednesday, November 06, 2013 2:46 PM > To: Mike Pence; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] On call hours required of staff > > No pay has been mentioned. The call hours are to be covered initially by one person . > > The tasks are potentially take care of anything occurring during those hours including stat specimens. The primary concern of management is that a refrigerator or the room temperature or humidity will go out of range when staff are not on site. There is a 24 hour monitor for environmental stuff that will call to the person's cell phone, and anything else that occurs when no one is scheduled in the area will result in a cell call. > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > From: mpence@grhs.net > > To: joelleweaver@hotmail.com; histonet@lists.utsouthwestern.edu > > Subject: RE: [Histonet] On call hours required of staff > > Date: Wed, 6 Nov 2013 20:34:17 +0000 > > > > Will the techs be paid call pay? What is being asked of the techs to do on call? > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelle weaver > > Sent: Wednesday, November 06, 2013 2:26 PM > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] On call hours required of staff > > > > Sorry if this re-posted- it got bounced back to me once. > > > > Please advise of the number of hours that your histology staff are scheduled to take on-call in addition to their 40 scheduled hours ( FT staff/hourly) ? > > If you have a maximum # of on call hours by your lab or HR policies, that would be most helpful. > > The proposal is to begin a usual 40 worked hours scheduled in the lab and then ~ 15 hours overnight on call for each weekday,( M-F), and potentially 8 hours or more each weekend day. I think this proposal is unreasonable, and 75 hours + 16 hours potential on call/week ( 91 hours on call?). > > I would appreciate your thoughts , comments? I hope to derail this with something more reasonable > > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** > The information contained in this message may be privileged and confidential > and protected from disclosure. If the reader of this message is not the > intended recipient, or an employee or agent responsible for delivering this > message to the intended recipient, you are hereby notified that any > dissemination, distribution or copying of this communication is strictly > prohibited. If you have received this communication in error, please notify > us immediately by replying to the message and deleting it from your computer. > Thank you. From joelleweaver <@t> hotmail.com Wed Nov 6 15:11:27 2013 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Wed Nov 6 15:11:30 2013 Subject: [Histonet] On call hours required of staff In-Reply-To: References: , , , , <25A4DE08332B19499904459F00AAACB719C704FEB6@EVS1.archildrens.org>, Message-ID: Is that an employment law you quote? There is only one person, and they are not able to work that many hours at this point in their life, or even willing to do so for any amount of money. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: mpence@grhs.net > To: HornHV@archildrens.org; joelleweaver@hotmail.com; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] On call hours required of staff > Date: Wed, 6 Nov 2013 21:08:29 +0000 > > No pay, no call! I don't think you could require your techs to take call without paying them for their on-call time. Maybe you have one or two techs that would not mind the extra money being on call. I remember back several years ago (maybe a million) when I was younger I would have taken anything for more money! > > -----Original Message----- > From: Horn, Hazel V [mailto:HornHV@archildrens.org] > Sent: Wednesday, November 06, 2013 3:01 PM > To: 'joelle weaver'; Mike Pence; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] On call hours required of staff > > I would not ask my techs to take call for no pay. We have to respond to call in 1 hour. The tech would not be able to make personal plans if they are on call. > > We are on call on long weekends. We take turns taking call and are paid call pay. If we get called in, it is overtime. On the rare occasion someone needs to work Saturday, one of us volunteer and are paid call pay and overtime. > > As a supervisor I am potentially on call all of the time. If our tissue processor alarms, I am called. I come in and I get call pay and overtime. (This is a rare occurrence) If I am unavailable, my senior tech answers calls. I also answer after hours calls from the lab. Usually a phone call takes care of it. > > Hazel Horn > Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital > 1 Children's Way | Slot 820| Little Rock, AR 72202 > 501.364.4240 direct | 501.364.1241 fax > hornhv@archildrens.org > archildrens.org > > > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelle weaver > Sent: Wednesday, November 06, 2013 2:46 PM > To: Mike Pence; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] On call hours required of staff > > No pay has been mentioned. The call hours are to be covered initially by one person . > > The tasks are potentially take care of anything occurring during those hours including stat specimens. The primary concern of management is that a refrigerator or the room temperature or humidity will go out of range when staff are not on site. There is a 24 hour monitor for environmental stuff that will call to the person's cell phone, and anything else that occurs when no one is scheduled in the area will result in a cell call. > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > From: mpence@grhs.net > > To: joelleweaver@hotmail.com; histonet@lists.utsouthwestern.edu > > Subject: RE: [Histonet] On call hours required of staff > > Date: Wed, 6 Nov 2013 20:34:17 +0000 > > > > Will the techs be paid call pay? What is being asked of the techs to do on call? > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelle > > weaver > > Sent: Wednesday, November 06, 2013 2:26 PM > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] On call hours required of staff > > > > Sorry if this re-posted- it got bounced back to me once. > > > > Please advise of the number of hours that your histology staff are scheduled to take on-call in addition to their 40 scheduled hours ( FT staff/hourly) ? > > If you have a maximum # of on call hours by your lab or HR policies, that would be most helpful. > > The proposal is to begin a usual 40 worked hours scheduled in the lab and then ~ 15 hours overnight on call for each weekday,( M-F), and potentially 8 hours or more each weekend day. I think this proposal is unreasonable, and 75 hours + 16 hours potential on call/week ( 91 hours on call?). > > I would appreciate your thoughts , comments? I hope to derail this > > with something more reasonable > > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** > The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. > Thank you. > > From pruegg <@t> ihctech.net Wed Nov 6 20:24:58 2013 From: pruegg <@t> ihctech.net (pruegg@ihctech.net) Date: Wed Nov 6 20:25:03 2013 Subject: [Histonet] FDA Disclaimer In-Reply-To: <450B7A81EDA0C54E97C53D60F00776C3232B4BB696@isexstore03> References: <450B7A81EDA0C54E97C53D60F00776C3232B4BB696@isexstore03> Message-ID: <00ce01cedb60$8e8c8440$aba58cc0$@ihctech.net> I would think so, Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E. Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 pruegghm@hotmail.com rueggihcconsultingpr@outlook.com ? This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie Sent: Tuesday, November 05, 2013 3:48 AM To: Histonet Post (histonet@lists.utsouthwestern.edu) Subject: [Histonet] FDA Disclaimer Good Morning... I have a question about the FDA disclaimer for Immuno's. If we are not doing the staining of Immuno's in our lab, but our Pathologists are interpreting those that are stained at our reference lab, are we still required to put the FDA disclaimer on our Path reports for those antibodies that require the disclaimer? Thanks so much!! Valerie Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.hannen@parrishmed.com ============= "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ============= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Wed Nov 6 20:29:45 2013 From: pruegg <@t> ihctech.net (pruegg@ihctech.net) Date: Wed Nov 6 20:29:50 2013 Subject: [Histonet] CD 34 antibody abcam (ab963) In-Reply-To: References: Message-ID: <00cf01cedb61$3a0d64b0$ae282e10$@ihctech.net> This is a tuff one CD34 will stain all precursor cells not just endothelial cells for vessels, there is an excellent CD31 for ms tissue from Dionova it is rat anti ms but not sure of what you could use for cd31 for rat and rabbits, but I know cd34 is not the way to go. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E. Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 pruegghm@hotmail.com rueggihcconsultingpr@outlook.com This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of tahseen Sent: Wednesday, November 06, 2013 5:34 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CD 34 antibody abcam (ab963) Hi All, We used CD 34 antibody abcam (ab963)with detection kit enVision to stain our samples from rats and rabbits. We need to stain vessels only but macrophages, fibroblast, collagen all are stained in other hand known +ve control (Tonsil) is perform good. Please help us that what should we do for IHC staining of our samples. Best regards Muhammad Tahseen Sr.Supervisor Histology SKMCH&RC Lahore _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Wed Nov 6 20:38:34 2013 From: pruegg <@t> ihctech.net (pruegg@ihctech.net) Date: Wed Nov 6 20:38:38 2013 Subject: [Histonet] Faxitron Xray digital imaging In-Reply-To: References: Message-ID: <00d001cedb62$74c616a0$5e5243e0$@ihctech.net> Years ago/maybe more than 25 we used a faxitron xray machine to measure bone calcification on research samples and samples being decaled, it was the best thing since sliced bread, had to go to the radiology dept to use it and was told they were dying off the market, loved it, so easy to use and so useful. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E. Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 pruegghm@hotmail.com rueggihcconsultingpr@outlook.com ? This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO Sent: Tuesday, November 05, 2013 9:47 AM To: Scott, Allison D; histonet Subject: RE: [Histonet] Faxitron Xray digital imaging We have used the Faxitron instrument for the past 3 years at multiple sites. Faxitron creates a digital image of a specimen quickly and safely and a t multiple magnifications. The instrument is placed in either Surgery or Surgical Pathology (SP) and we use it primarily on breast cases to assist in orientation and gross dissection, identifying radioactive seeds or locating micro calcification. The instrument is easy to use, safe and you can connect to multiple LIS systems and a Hospital PACS. There is no code to charge for use when used in SP, only when used by Radiology or Surgery. There is only one company that manufactures and sells the instrument: Faxitron Bioptics, LLC Tucson, AZ 877-910-0030 The are willing to bring a unit to your site for demonstration and you may be able to talk them into leaving for a evaluation. William DeSalvo, BS HTL(ASCP) > From: Allison.Scott@harrishealth.org > To: histonet@lists.utsouthwestern.edu > Date: Tue, 5 Nov 2013 16:00:11 +0000 > Subject: [Histonet] Faxitron Xray digital imaging > > Hello to all in histoland. Our chief pathologist is interested in getting a faxitron xray digital imaging machine. Is anyone out there using this technology and can you suggest a company in which to order one from. Any help in this will be greatly appreciated. > > > > > Allison Scott HT(ASCP) > Supervisor, Histology Lab > LBJ Hospital > Harris Health System > Office: 713-566-2148 > Lab: 713-566-5287 > > > CONFIDENTIALITY NOTICE: > If you have received this e-mail in error, please immediately notify > the sender by return e-mail and delete this e-mail and any attachments > from your computer system. > > To the extent the information in this e-mail and any attachments > contain protected health information as defined by the Health > Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL > 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and > Safety Code, it is confidential and/or privileged. This e-mail may > also be confidential and/or privileged under Texas law. The e-mail is > for the use of only the individual or entity named above. If you are > not the intended recipient, or any authorized representative of the > intended recipient, you are hereby notified that any review, > dissemination or copying of this e-mail and its attachments is strictly prohibited. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Susan.Walzer <@t> HCAHealthcare.com Thu Nov 7 02:12:28 2013 From: Susan.Walzer <@t> HCAHealthcare.com (Susan.Walzer@HCAHealthcare.com) Date: Thu Nov 7 02:12:40 2013 Subject: [Histonet] Faxitron Xray digital imaging In-Reply-To: <00d001cedb62$74c616a0$5e5243e0$@ihctech.net> References: <00d001cedb62$74c616a0$5e5243e0$@ihctech.net> Message-ID: <4BF03F5404EBDE409AF9232DA74B9DED2FAB2A2984@FWDCWPMSGCMS09.hca.corpad.net> Right, it's the only way to be sure bone is really decaled enough. We also used to use it to find calcium in lumpectomies. I am sure we were not doing proper monitoring of it though as it was in our dept. and radiology never checked it. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of pruegg@ihctech.net Sent: Wednesday, November 06, 2013 9:39 PM To: 'WILLIAM DESALVO'; 'Scott, Allison D'; 'histonet' Subject: RE: [Histonet] Faxitron Xray digital imaging Years ago/maybe more than 25 we used a faxitron xray machine to measure bone calcification on research samples and samples being decaled, it was the best thing since sliced bread, had to go to the radiology dept to use it and was told they were dying off the market, loved it, so easy to use and so useful. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E. Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 pruegghm@hotmail.com rueggihcconsultingpr@outlook.com ? This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO Sent: Tuesday, November 05, 2013 9:47 AM To: Scott, Allison D; histonet Subject: RE: [Histonet] Faxitron Xray digital imaging We have used the Faxitron instrument for the past 3 years at multiple sites. Faxitron creates a digital image of a specimen quickly and safely and a t multiple magnifications. The instrument is placed in either Surgery or Surgical Pathology (SP) and we use it primarily on breast cases to assist in orientation and gross dissection, identifying radioactive seeds or locating micro calcification. The instrument is easy to use, safe and you can connect to multiple LIS systems and a Hospital PACS. There is no code to charge for use when used in SP, only when used by Radiology or Surgery. There is only one company that manufactures and sells the instrument: Faxitron Bioptics, LLC Tucson, AZ 877-910-0030 The are willing to bring a unit to your site for demonstration and you may be able to talk them into leaving for a evaluation. William DeSalvo, BS HTL(ASCP) > From: Allison.Scott@harrishealth.org > To: histonet@lists.utsouthwestern.edu > Date: Tue, 5 Nov 2013 16:00:11 +0000 > Subject: [Histonet] Faxitron Xray digital imaging > > Hello to all in histoland. Our chief pathologist is interested in getting a faxitron xray digital imaging machine. Is anyone out there using this technology and can you suggest a company in which to order one from. Any help in this will be greatly appreciated. > > > > > Allison Scott HT(ASCP) > Supervisor, Histology Lab > LBJ Hospital > Harris Health System > Office: 713-566-2148 > Lab: 713-566-5287 > > > CONFIDENTIALITY NOTICE: > If you have received this e-mail in error, please immediately notify > the sender by return e-mail and delete this e-mail and any attachments > from your computer system. > > To the extent the information in this e-mail and any attachments > contain protected health information as defined by the Health > Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL > 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and > Safety Code, it is confidential and/or privileged. This e-mail may > also be confidential and/or privileged under Texas law. The e-mail is > for the use of only the individual or entity named above. If you are > not the intended recipient, or any authorized representative of the > intended recipient, you are hereby notified that any review, > dissemination or copying of this e-mail and its attachments is strictly prohibited. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From avistarop <@t> ffyb.uba.ar Thu Nov 7 06:29:39 2013 From: avistarop <@t> ffyb.uba.ar (avistarop@ffyb.uba.ar) Date: Thu Nov 7 06:54:54 2013 Subject: [Histonet] submissions of list Message-ID: Send Histonet mailing list submissions to From ecrespo <@t> cmblabs.com Thu Nov 7 11:14:45 2013 From: ecrespo <@t> cmblabs.com (Ed Crespo) Date: Thu Nov 7 11:15:39 2013 Subject: [Histonet] India Ink for inking surgical margin borders In-Reply-To: <9E2D36CE2D7CBA4A94D9B22E8328A3BA27E31F7135@NADCWPMSGCMS03.hca.corpad.net> References: <21511BF5-BA90-4392-A9B4-FF159441BC5A@cmblabs.com> <9E2D36CE2D7CBA4A94D9B22E8328A3BA27E31F7135@NADCWPMSGCMS03.hca.corpad.net> Message-ID: <3604FD97-6D65-4B3C-B33E-6A2C75E4533C@cmblabs.com> Hi Wanda, Can you tell me the brand name or take a pic of the india ink you got at the art store? I bought one from dick blick art supply. Although it works (AND WAS CHEAP...LOL), we're always looking for a better, darker ink. Any brand suggestions would be great. Thank you in advance. ED Ed Crespo, CT(ASCP) Anatomic Pathology Manager Safety Officer 10700 Walker Street Cypress, CA 90630 phone: 714 880.3330 fax: 714 816.1511 email: ecrespo@cmblabs.com cmblabs.com The contents of this e-mail message, including any attachments, are intended solely for the use of the person or entity to whom this e-mail is addressed. It contains information that may be privileged, proprietary, confidential, and protected from disclosure by applicable state and federal law. Any Protected Health Information (PHI) contained in this email is HIGHLY CONFIDENTIAL. It is intended for the exclusive use of the addressee. It is to be used only to aid in providing specific healthcare services to the patient(s). Any other use is a violation of Federal Law (HIPAA) and will be reported as such. If you are not the intended recipient of this message, or the employee or agent responsible for delivering it to the intended recipient, you are hereby advised that reading, disseminating, distributing, use, or copying of the contents of this message is strictly prohibited. If you have received this message in error, or are not the named recipient(s), please notify the sender immediately by reply e-mail or by phone at (714) 657-7369 and delete this message and any attachments from your computer and any archival/backup copies. Thank you. On Oct 11, 2013, at 8:37 AM, wrote: > We sued India Ink from an art supply store for years. > > WANDA G. SMITH, HTL(ASCP)HT > Pathology Supervisor > TRIDENT MEDICAL CENTER > 9330 Medical Plaza Drive > Charleston, SC 29406 > 843-847-4586 > 843-847-4296 fax > > This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ed Crespo > Sent: Thursday, October 10, 2013 12:53 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] India Ink for inking surgical margin borders > > I normally purchase India Ink from one of our vendors, but know it's also sold and used at artist supply shops. Does anyone know if I can used the artist india ink for Pathology use? Really, the only issue would be if the ink stays on the tissue during processing right? Please advise. > > Ed Crespo, CT(ASCP) > Anatomic Pathology Manager > Safety Officer > > > 10700 Walker Street > Cypress, CA 90630 > phone: 714 880.3330 > fax: 714 816.1511 > email: ecrespo@cmblabs.com > cmblabs.com > > > The contents of this e-mail message, including any attachments, are intended solely for the use of the person or entity to whom this e-mail is addressed. It contains information that may be privileged, proprietary, confidential, and protected from disclosure by applicable state and federal law. Any Protected Health Information (PHI) contained in this email is HIGHLY CONFIDENTIAL. It is intended for the exclusive use of the addressee. It is to be used only to aid in providing specific healthcare services to the patient(s). Any other use is a violation of Federal Law (HIPAA) and will be reported as such. If you are not the intended recipient of this message, or the employee or agent responsible for delivering it to the intended recipient, you are hereby advised that reading, disseminating, distributing, use, or copying of the contents of this message is strictly prohibited. If you have received this message in error, or are not the named recipient(s), please notify the sender immediately by reply e-mail or by phone at (714) 657-7369 and delete this message and any attachments from your computer and any archival/backup copies. Thank you. > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From barbara.tibbs <@t> accuratediagnosticlabs.com Thu Nov 7 11:29:18 2013 From: barbara.tibbs <@t> accuratediagnosticlabs.com (Barbara Tibbs) Date: Thu Nov 7 11:29:27 2013 Subject: [Histonet] India Ink for inking surgical margin borders In-Reply-To: <3604FD97-6D65-4B3C-B33E-6A2C75E4533C@cmblabs.com> References: <21511BF5-BA90-4392-A9B4-FF159441BC5A@cmblabs.com> <9E2D36CE2D7CBA4A94D9B22E8328A3BA27E31F7135@NADCWPMSGCMS03.hca.corpad.net>, <3604FD97-6D65-4B3C-B33E-6A2C75E4533C@cmblabs.com> Message-ID: FYI, laundry bluing works great to ink specimens and it's very cheap. Most grocery stores sell it. It may not be as dark as you like but if you need two colors for inking oriented specimens you could use it in addition to the India Ink. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.tibbs@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Ed Crespo Sent: Thursday, November 07, 2013 3:14 PM To: Wanda.Smith@HCAhealthcare.com Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] India Ink for inking surgical margin borders Hi Wanda, Can you tell me the brand name or take a pic of the india ink you got at the art store? I bought one from dick blick art supply. Although it works (AND WAS CHEAP...LOL), we're always looking for a better, darker ink. Any brand suggestions would be great. Thank you in advance. ED Ed Crespo, CT(ASCP) Anatomic Pathology Manager Safety Officer 10700 Walker Street Cypress, CA 90630 phone: 714 880.3330 fax: 714 816.1511 email: ecrespo@cmblabs.com cmblabs.com The contents of this e-mail message, including any attachments, are intended solely for the use of the person or entity to whom this e-mail is addressed. It contains information that may be privileged, proprietary, confidential, and protected from disclosure by applicable state and federal law. Any Protected Health Information (PHI) contained in this email is HIGHLY CONFIDENTIAL. It is intended for the exclusive use of the addressee. It is to be used only to aid in providing specific healthcare services to the patient(s). Any other use is a violation of Federal Law (HIPAA) and will be reported as such. If you are not the intended recipient of this message, or the employee or agent responsible for delivering it to the intended recipient, you are hereby advised that reading, disseminating, distributing, use, or copying of the contents of this message is strictly prohibited. If you have received this message in error, or are not the named recipient(s), please notify the sender immediately by reply e-mail or by phone at (714) 657-7369 and delete this message and any attachments from your computer and any archival/backup copies. Thank you. On Oct 11, 2013, at 8:37 AM, wrote: > We sued India Ink from an art supply store for years. > > WANDA G. SMITH, HTL(ASCP)HT > Pathology Supervisor > TRIDENT MEDICAL CENTER > 9330 Medical Plaza Drive > Charleston, SC 29406 > 843-847-4586 > 843-847-4296 fax > > This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ed Crespo > Sent: Thursday, October 10, 2013 12:53 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] India Ink for inking surgical margin borders > > I normally purchase India Ink from one of our vendors, but know it's also sold and used at artist supply shops. Does anyone know if I can used the artist india ink for Pathology use? Really, the only issue would be if the ink stays on the tissue during processing right? Please advise. > > Ed Crespo, CT(ASCP) > Anatomic Pathology Manager > Safety Officer > > > 10700 Walker Street > Cypress, CA 90630 > phone: 714 880.3330 > fax: 714 816.1511 > email: ecrespo@cmblabs.com > cmblabs.com > > > The contents of this e-mail message, including any attachments, are intended solely for the use of the person or entity to whom this e-mail is addressed. It contains information that may be privileged, proprietary, confidential, and protected from disclosure by applicable state and federal law. Any Protected Health Information (PHI) contained in this email is HIGHLY CONFIDENTIAL. It is intended for the exclusive use of the addressee. It is to be used only to aid in providing specific healthcare services to the patient(s). Any other use is a violation of Federal Law (HIPAA) and will be reported as such. If you are not the intended recipient of this message, or the employee or agent responsible for delivering it to the intended recipient, you are hereby advised that reading, disseminating, distributing, use, or copying of the contents of this message is strictly prohibited. If you have received this message in error, or are not the named recipient(s), please notify the sender immediately by reply e-mail or by phone at (714) 657-7369 and delete this message and any attachments from your computer and any archival/backup copies. Thank you. > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Joyce.Weems <@t> emoryhealthcare.org Thu Nov 7 11:31:52 2013 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Thu Nov 7 11:32:13 2013 Subject: [Histonet] India Ink for inking surgical margin borders In-Reply-To: <3604FD97-6D65-4B3C-B33E-6A2C75E4533C@cmblabs.com> References: <21511BF5-BA90-4392-A9B4-FF159441BC5A@cmblabs.com> <9E2D36CE2D7CBA4A94D9B22E8328A3BA27E31F7135@NADCWPMSGCMS03.hca.corpad.net> <3604FD97-6D65-4B3C-B33E-6A2C75E4533C@cmblabs.com> Message-ID: Higgins is the best brand in my opinion - and, of course, the most expensive! Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ed Crespo Sent: Thursday, November 07, 2013 12:15 PM To: Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] India Ink for inking surgical margin borders Hi Wanda, Can you tell me the brand name or take a pic of the india ink you got at the art store? I bought one from dick blick art supply. Although it works (AND WAS CHEAP...LOL), we're always looking for a better, darker ink. Any brand suggestions would be great. Thank you in advance. ED Ed Crespo, CT(ASCP) Anatomic Pathology Manager Safety Officer 10700 Walker Street Cypress, CA 90630 phone: 714 880.3330 fax: 714 816.1511 email: ecrespo@cmblabs.com cmblabs.com The contents of this e-mail message, including any attachments, are intended solely for the use of the person or entity to whom this e-mail is addressed. It contains information that may be privileged, proprietary, confidential, and protected from disclosure by applicable state and federal law. Any Protected Health Information (PHI) contained in this email is HIGHLY CONFIDENTIAL. It is intended for the exclusive use of the addressee. It is to be used only to aid in providing specific healthcare services to the patient(s). Any other use is a violation of Federal Law (HIPAA) and will be reported as such. If you are not the intended recipient of this message, or the employee or agent responsible for delivering it to the intended recipient, you are hereby advised that reading, disseminating, distributing, use, or copying of the contents of this message is strictly prohibited. If you have received this message in error, or are not the named recipient(s), please notify the sender immediately by reply e-mail or by phone at (714) 657-7369 and delete this message and any attachments from your computer and any archival/backup copies. Thank you. On Oct 11, 2013, at 8:37 AM, wrote: > We sued India Ink from an art supply store for years. > > WANDA G. SMITH, HTL(ASCP)HT > Pathology Supervisor > TRIDENT MEDICAL CENTER > 9330 Medical Plaza Drive > Charleston, SC 29406 > 843-847-4586 > 843-847-4296 fax > > This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ed Crespo > Sent: Thursday, October 10, 2013 12:53 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] India Ink for inking surgical margin borders > > I normally purchase India Ink from one of our vendors, but know it's also sold and used at artist supply shops. Does anyone know if I can used the artist india ink for Pathology use? Really, the only issue would be if the ink stays on the tissue during processing right? Please advise. > > Ed Crespo, CT(ASCP) > Anatomic Pathology Manager > Safety Officer > > > 10700 Walker Street > Cypress, CA 90630 > phone: 714 880.3330 > fax: 714 816.1511 > email: ecrespo@cmblabs.com > cmblabs.com > > > The contents of this e-mail message, including any attachments, are intended solely for the use of the person or entity to whom this e-mail is addressed. It contains information that may be privileged, proprietary, confidential, and protected from disclosure by applicable state and federal law. Any Protected Health Information (PHI) contained in this email is HIGHLY CONFIDENTIAL. It is intended for the exclusive use of the addressee. It is to be used only to aid in providing specific healthcare services to the patient(s). Any other use is a violation of Federal Law (HIPAA) and will be reported as such. If you are not the intended recipient of this message, or the employee or agent responsible for delivering it to the intended recipient, you are hereby advised that reading, disseminating, distributing, use, or copying of the contents of this message is strictly prohibited. If you have received this message in error, or are not the named recipient(s), please notify the sender immediately by reply e-mail or by phone at (714) 657-7369 and delete this message and any attachments from your computer and any archival/backup copies. Thank you. > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From histotalk <@t> yahoo.com Thu Nov 7 11:55:47 2013 From: histotalk <@t> yahoo.com (David Kemler) Date: Thu Nov 7 11:55:57 2013 Subject: [Histonet] Vinnie Della Speranza on HistoTALK Message-ID: <1383846947.74669.YahooMailNeo@web121505.mail.ne1.yahoo.com> Hello All - ? I case you haven't heard, Vinnie Della Speranza is our guest on HostoTALK http://www.histotalk.com/. As always, Vinnie shares some great information and opinions. Love having him on! ? Syndication problems delayed?Program #41 from posting on its normally scheduled time. ? This Sunday, November 17th, Pam Barker?from RELIA Business Solutions will be our guest.?Pam will be sharing info?about landing that "fantastic" new job.?It all happens on HistoTALK http://www.histotalk.com/ ? ? Yours, David From relia1 <@t> earthlink.net Thu Nov 7 13:23:06 2013 From: relia1 <@t> earthlink.net (Pam Barker) Date: Thu Nov 7 13:23:14 2013 Subject: [Histonet] RELIA HOT JOB ALERT IHC tech Days NY,NY Message-ID: <00ea01cedbee$c9d28700$5d779500$@earthlink.net> Hi Histonetters!! Wow tomorrow is TGIF where did the week go? Well before I post this elsewhere thought I would give you guys a preview. Here is a new job that I am just starting to work on. Let me know if you are interested OR if you know someone who is. Remember if I place someone you refer to me I will pay you a referral fee and that is going to come in pretty handy for Christmas shopping!! Here is the posting: Immunohistochemistry Specialist - Leading Prestigious Facility in NY, NY RELIA Solutions the nation's only recruiting firm specializing exclusively in the permanent placement of histology professionals has been engaged by a prominent highly regarded hospital located in NY, NY in their search for an IHC specialist. This is a full time permanent position and my client offers excellent compensation, benefits and a great work environment. NYS clinical licensure and 1-2 years of IHC experience is required. For more information please contact Pam Barker at relia1@earthlink.net or toll free at 866-607-3542. Keywords: histology, histologist, histotechnologist, histotechnician, immunohistochemistry, ihc Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From kathryn.perkinson <@t> duke.edu Thu Nov 7 14:58:20 2013 From: kathryn.perkinson <@t> duke.edu (Kathryn Perkinson) Date: Thu Nov 7 14:58:37 2013 Subject: [Histonet] Image Cytometry Laboratory At Duke Health System - open positions In-Reply-To: <20131105180138.079BE42D17BA_2793282F@mail-gw-05.oit.duke.edu> References: <20131105180138.079BE42D17BA_2793282F@mail-gw-05.oit.duke.edu> Message-ID: <6320921BB335BB439ED0D88E340799F219A77CEE@vmw-excmbd2s03.dhe.duke.edu> Molecular Technologist II position open in Image Cytometry Laboratory. Monday through Friday 9 am to 5:30 pm. Education: 4 year degree in Biological science, Certification: HT, HTL, or CG. Experience in immunohistochemistry and fluorescence in situ hybridization needed. Please send resume to: Kathryn R. Perkinson, BS, HTL(ASCP) Manager, Molecular Pathology Duke University Health System Rm. #4710 Duke South, Yellow Zone Durham, North Carolina 27710 P: 919.684.5822 F: 919.684.8117 HIPAA Privacy Notification: This message and any accompanying documents are covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, and contain information intended for the specific individual (s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying or the taking of any action based on the contents of this information is strictly prohibited . If you have received this communication in error, please notify us immediately by e-mail , and delete the original message. From Timothy.Morken <@t> ucsfmedctr.org Thu Nov 7 17:02:45 2013 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Thu Nov 7 17:03:10 2013 Subject: [Histonet] Histo arcania ... Reticulum vs Reticulin Message-ID: <761E2B5697F795489C8710BCC72141FF0AFA54@ex07.net.ucsf.edu> Oh Great Histonet, how do you describe the difference, if any, between the terms "reticulum" and "reticulin." Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center Box 1656 505 Parnassus Ave San Francisco, CA 94143 USA 415.353.1266 (office) tim.morken@ucsfmedctr.org From chapcl <@t> yahoo.com Thu Nov 7 17:24:35 2013 From: chapcl <@t> yahoo.com (Will Chappell) Date: Thu Nov 7 17:24:52 2013 Subject: [Histonet] Histo arcania ... Reticulum vs Reticulin In-Reply-To: <761E2B5697F795489C8710BCC72141FF0AFA54@ex07.net.ucsf.edu> References: <761E2B5697F795489C8710BCC72141FF0AFA54@ex07.net.ucsf.edu> Message-ID: <6B3FF475-9D6C-4AB1-9C41-1F8C1E0BB3DA@yahoo.com> Reticulin is a type of fiber in connective tissue composed of type III collage secreted by reticular cells. Reticulum pertains to the endoplasmic reticulum, the second chamber of the alimentary canal of a ruminant animal, or (less frequent) the plural of reticular cell. Will Chappell Sent from my iPhone > On Nov 7, 2013, at 3:02 PM, "Morken, Timothy" wrote: > > Oh Great Histonet, how do you describe the difference, if any, between the terms "reticulum" and "reticulin." > > > > Tim Morken > Supervisor, Electron Microscopy and Neuromuscular Special Studies > UC San Francisco Medical Center > Box 1656 > 505 Parnassus Ave > San Francisco, CA 94143 > USA > > 415.353.1266 (office) > tim.morken@ucsfmedctr.org > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Joyce.Weems <@t> emoryhealthcare.org Thu Nov 7 17:48:59 2013 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Thu Nov 7 17:49:06 2013 Subject: [Histonet] RE: Histo arcania ... Reticulum vs Reticulin In-Reply-To: <761E2B5697F795489C8710BCC72141FF0AFA54@ex07.net.ucsf.edu> References: <761E2B5697F795489C8710BCC72141FF0AFA54@ex07.net.ucsf.edu> Message-ID: I have seen them and have been told they are the same and interchangeable. I've never really looked into it, tho. It will be good to learn! Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Thursday, November 07, 2013 6:03 PM To: Histonet Subject: [Histonet] Histo arcania ... Reticulum vs Reticulin Oh Great Histonet, how do you describe the difference, if any, between the terms "reticulum" and "reticulin." Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center Box 1656 505 Parnassus Ave San Francisco, CA 94143 USA 415.353.1266 (office) tim.morken@ucsfmedctr.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From barryrittman <@t> gmail.com Thu Nov 7 18:24:13 2013 From: barryrittman <@t> gmail.com (Barry Rittman) Date: Thu Nov 7 18:24:16 2013 Subject: [Histonet] RE: Histo arcania ... Reticulum vs Reticulin In-Reply-To: References: <761E2B5697F795489C8710BCC72141FF0AFA54@ex07.net.ucsf.edu> Message-ID: Reticulin is a type of collagenous fiber that is generally argyrophilic and first recognized as a type of collagen with the advent of electron microscopy. Associated with basement membranes and with fiber networks in bone marrow, lymph nodes etc. Reticulum in* anatomy* refers to a network. In* histology* it is usually used to describe the endoplasmic reticulum organelles in cells, comprised or smooth and rough endoplasmic reticulum and involved in protein synthesis and modification. Barry On Thu, Nov 7, 2013 at 5:48 PM, Weems, Joyce K. < Joyce.Weems@emoryhealthcare.org> wrote: > I have seen them and have been told they are the same and interchangeable. > I've never really looked into it, tho. It will be good to learn! > > Joyce Weems > Pathology Manager > 678-843-7376 Phone > 678-843-7831 Fax > joyce.weems@emoryhealthcare.org > > > > www.saintjosephsatlanta.org > 5665 Peachtree Dunwoody Road > Atlanta, GA 30342 > > This e-mail, including any attachments is the property of Saint Joseph's > Hospital and is intended for the sole use of the intended recipient(s). It > may contain information that is privileged and confidential. Any > unauthorized review, use, disclosure, or distribution is prohibited. If you > are not the intended recipient, please delete this message, and reply to > the sender regarding the error in a separate email. > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy > Sent: Thursday, November 07, 2013 6:03 PM > To: Histonet > Subject: [Histonet] Histo arcania ... Reticulum vs Reticulin > > Oh Great Histonet, how do you describe the difference, if any, between the > terms "reticulum" and "reticulin." > > > > Tim Morken > Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San > Francisco Medical Center Box 1656 > 505 Parnassus Ave > San Francisco, CA 94143 > USA > > 415.353.1266 (office) > tim.morken@ucsfmedctr.org > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ________________________________ > > This e-mail message (including any attachments) is for the sole use of > the intended recipient(s) and may contain confidential and privileged > information. If the reader of this message is not the intended > recipient, you are hereby notified that any dissemination, distribution > or copying of this message (including any attachments) is strictly > prohibited. > > If you have received this message in error, please contact > the sender by reply e-mail message and destroy all copies of the > original message (including attachments). > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Timothy.Morken <@t> ucsfmedctr.org Thu Nov 7 18:35:18 2013 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Thu Nov 7 18:35:28 2013 Subject: FW: [Histonet] RE: Histo arcania ... Reticulum vs Reticulin In-Reply-To: <761E2B5697F795489C8710BCC72141FF0AFAE8@ex07.net.ucsf.edu> References: <761E2B5697F795489C8710BCC72141FF0AFA54@ex07.net.ucsf.edu> <761E2B5697F795489C8710BCC72141FF0AFAE8@ex07.net.ucsf.edu> Message-ID: <761E2B5697F795489C8710BCC72141FF0AFAF4@ex07.net.ucsf.edu> Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: Morken, Timothy Sent: Thursday, November 07, 2013 4:35 PM To: 'Barry Rittman' Subject: RE: [Histonet] RE: Histo arcania ... Reticulum vs Reticulin Not to pick on Barry, but here is some other info These books refer to "reticulum" stain in reference to staining Reticular fibers: Sheehan Lillie Pearse Carson AFIP manual All stain kit vendors I have looked at These books refer to "Reticulin" stain in reference to staining Reticular fibers: Histology, A text and Atlas Basic Pathology Color Atlas of Histology Histology for Pathologists Bancroft's Histological Methods Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barry Rittman Sent: Thursday, November 07, 2013 4:24 PM Cc: Histonet Subject: Re: [Histonet] RE: Histo arcania ... Reticulum vs Reticulin Reticulin is a type of collagenous fiber that is generally argyrophilic and first recognized as a type of collagen with the advent of electron microscopy. Associated with basement membranes and with fiber networks in bone marrow, lymph nodes etc. Reticulum in* anatomy* refers to a network. In* histology* it is usually used to describe the endoplasmic reticulum organelles in cells, comprised or smooth and rough endoplasmic reticulum and involved in protein synthesis and modification. Barry On Thu, Nov 7, 2013 at 5:48 PM, Weems, Joyce K. < Joyce.Weems@emoryhealthcare.org> wrote: > I have seen them and have been told they are the same and interchangeable. > I've never really looked into it, tho. It will be good to learn! > > Joyce Weems > Pathology Manager > 678-843-7376 Phone > 678-843-7831 Fax > joyce.weems@emoryhealthcare.org > > > > www.saintjosephsatlanta.org > 5665 Peachtree Dunwoody Road > Atlanta, GA 30342 > > This e-mail, including any attachments is the property of Saint > Joseph's Hospital and is intended for the sole use of the intended > recipient(s). It may contain information that is privileged and > confidential. Any unauthorized review, use, disclosure, or > distribution is prohibited. If you are not the intended recipient, > please delete this message, and reply to the sender regarding the error in a separate email. > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, > Timothy > Sent: Thursday, November 07, 2013 6:03 PM > To: Histonet > Subject: [Histonet] Histo arcania ... Reticulum vs Reticulin > > Oh Great Histonet, how do you describe the difference, if any, between > the terms "reticulum" and "reticulin." > > > > Tim Morken > Supervisor, Electron Microscopy and Neuromuscular Special Studies UC > San Francisco Medical Center Box 1656 > 505 Parnassus Ave > San Francisco, CA 94143 > USA > > 415.353.1266 (office) > tim.morken@ucsfmedctr.org > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ________________________________ > > This e-mail message (including any attachments) is for the sole use of > the intended recipient(s) and may contain confidential and privileged > information. If the reader of this message is not the intended > recipient, you are hereby notified that any dissemination, > distribution or copying of this message (including any attachments) is > strictly prohibited. > > If you have received this message in error, please contact the sender > by reply e-mail message and destroy all copies of the original message > (including attachments). > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From chapcl <@t> yahoo.com Thu Nov 7 18:38:47 2013 From: chapcl <@t> yahoo.com (Will Chappell) Date: Thu Nov 7 18:38:55 2013 Subject: [Histonet] RE: Histo arcania ... Reticulum vs Reticulin In-Reply-To: <761E2B5697F795489C8710BCC72141FF0AFAF4@ex07.net.ucsf.edu> References: <761E2B5697F795489C8710BCC72141FF0AFA54@ex07.net.ucsf.edu> <761E2B5697F795489C8710BCC72141FF0AFAE8@ex07.net.ucsf.edu> <761E2B5697F795489C8710BCC72141FF0AFAF4@ex07.net.ucsf.edu> Message-ID: Seems like our four-histotechs were the worst offenders for misuse. I believe Barry is right. A retic stain is a silver stain of a reticulum matrix of reticulin fibers (produced, of course, by reticular cells). Sent from my iPhone > On Nov 7, 2013, at 4:35 PM, "Morken, Timothy" wrote: > > > > Tim Morken > Supervisor, Electron Microscopy and Neuromuscular Special Studies > UC San Francisco Medical Center > San Francisco, CA > > > -----Original Message----- > From: Morken, Timothy > Sent: Thursday, November 07, 2013 4:35 PM > To: 'Barry Rittman' > Subject: RE: [Histonet] RE: Histo arcania ... Reticulum vs Reticulin > > Not to pick on Barry, but here is some other info > > These books refer to "reticulum" stain in reference to staining Reticular fibers: > Sheehan > Lillie > Pearse > Carson > AFIP manual > All stain kit vendors I have looked at > > > These books refer to "Reticulin" stain in reference to staining Reticular fibers: > > Histology, A text and Atlas > Basic Pathology > Color Atlas of Histology > Histology for Pathologists > Bancroft's Histological Methods > > Tim Morken > Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barry Rittman > Sent: Thursday, November 07, 2013 4:24 PM > Cc: Histonet > Subject: Re: [Histonet] RE: Histo arcania ... Reticulum vs Reticulin > > Reticulin is a type of collagenous fiber that is generally argyrophilic and first recognized as a type of collagen with the advent of electron microscopy. Associated with basement membranes and with fiber networks in bone marrow, lymph nodes etc. > Reticulum in* anatomy* refers to a network. > In* histology* it is usually used to describe the endoplasmic reticulum organelles in cells, comprised or smooth and rough endoplasmic reticulum and involved in protein synthesis and modification. > Barry > > >> On Thu, Nov 7, 2013 at 5:48 PM, Weems, Joyce K. < Joyce.Weems@emoryhealthcare.org> wrote: >> >> I have seen them and have been told they are the same and interchangeable. >> I've never really looked into it, tho. It will be good to learn! >> >> Joyce Weems >> Pathology Manager >> 678-843-7376 Phone >> 678-843-7831 Fax >> joyce.weems@emoryhealthcare.org >> >> >> >> www.saintjosephsatlanta.org >> 5665 Peachtree Dunwoody Road >> Atlanta, GA 30342 >> >> This e-mail, including any attachments is the property of Saint >> Joseph's Hospital and is intended for the sole use of the intended >> recipient(s). It may contain information that is privileged and >> confidential. Any unauthorized review, use, disclosure, or >> distribution is prohibited. If you are not the intended recipient, >> please delete this message, and reply to the sender regarding the error in a separate email. >> >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu [mailto: >> histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, >> Timothy >> Sent: Thursday, November 07, 2013 6:03 PM >> To: Histonet >> Subject: [Histonet] Histo arcania ... Reticulum vs Reticulin >> >> Oh Great Histonet, how do you describe the difference, if any, between >> the terms "reticulum" and "reticulin." >> >> >> >> Tim Morken >> Supervisor, Electron Microscopy and Neuromuscular Special Studies UC >> San Francisco Medical Center Box 1656 >> 505 Parnassus Ave >> San Francisco, CA 94143 >> USA >> >> 415.353.1266 (office) >> tim.morken@ucsfmedctr.org >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> ________________________________ >> >> This e-mail message (including any attachments) is for the sole use of >> the intended recipient(s) and may contain confidential and privileged >> information. If the reader of this message is not the intended >> recipient, you are hereby notified that any dissemination, >> distribution or copying of this message (including any attachments) is >> strictly prohibited. >> >> If you have received this message in error, please contact the sender >> by reply e-mail message and destroy all copies of the original message >> (including attachments). >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DianaRip1 <@t> aol.com Thu Nov 7 19:07:56 2013 From: DianaRip1 <@t> aol.com (DianaRip1@aol.com) Date: Thu Nov 7 19:07:59 2013 Subject: [Histonet] Bay Area Hospital Autopsies Message-ID: I am looking for some information about what everyone does for Autopsies at their Hospital facility (particularly in the San Francisco Bay Area). Do you have Deniers that help? How much of the autopsy do your Deniers do? Where do you get your Deniers from and how much training have they had? How much do your Pathologists actually participate in the autopsy? Do you contract your Autopsies out? Thanks so much for any help you can give. Diana Ripley Pathology Supervisor John Muir Health _diana.ripley@johnmuirhealth.com_ (mailto:diana.ripley@johnmuirhealth.com) _dianarip1@gmail.com_ (mailto:dianarip1@gmail.com) 925-246-3999 From Pat.Patterson <@t> propath.com Thu Nov 7 19:40:09 2013 From: Pat.Patterson <@t> propath.com (Pat Patterson) Date: Thu Nov 7 19:40:13 2013 Subject: [Histonet] IHC Position Dallas Message-ID: <6DCB8B92D0138244B56CE8EACE0D458D013113@Mail.propathlab.com> IMMUNOHISTOCHEMISTRY TECHNICIAN ProPath, a progressive, CAP accredited, high-volume pathology practice in Dallas, Texas is seeking an Immunohistochemistry Technician for its' Immunohistochemistry Lab. Responsibilities include slide preparation (paraffin and frozen sections), IHC staining using our unique manual system, antibody titer preparation, equipment maintenance, supply/reagent inventory maintenance, and QC/QA recording. The ideal candidate will have a minimum of 5 years Histology experience with paraffin microtomy with a variety of different tissue types, prefer at least 3 years immunohistochemistry, immunofluorescence or in situ hybridization and frozen section experience. Technical Lead/Coordinator experience is a plus. Working knowledge of IHC theory required, hands on IHC performance desired. If using an automated system we'll easily train you on our manual system. HT (ASCP) or QIHC desired. The hours for the position are 4:00 p.m. to 12:30 a.m. Monday through Friday. ProPath utilizes leading technology and is a quality oriented pathology laboratory. Benefits include medical, dental, Short and Long Term Disability insurance, a matched 401K plan and more! Don't Follow the Leader! Join the Leader! To apply, please visit www.propath.com EOE Accessibility Accommodations If you require an accommodation to navigate or apply to our careers site, please send your request to accessibility@propath.com. Pat Patterson, HTL(ASCP) Supervisor, Immunohistochemistry ProPath - The Leader in Pathology Services 1355 River Bend Drive Dallas, TX 75247 214-237-1700 x 2027 214-237-1730 fax To learn more about ProPath, please visit http://www.ProPath.com This electronic message is intended to be for the use only of the named recipient and may contain information that is confidential or privileged. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this message in error or are not the named recipient, please notify us immediately by contacting the sender at the electronic mail address noted above, and delete and destroy all copies of this message. From rjbuesa <@t> yahoo.com Fri Nov 8 08:46:27 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Nov 8 08:46:35 2013 Subject: [Histonet] Histo arcania ... Reticulum vs Reticulin In-Reply-To: <761E2B5697F795489C8710BCC72141FF0AFA54@ex07.net.ucsf.edu> References: <761E2B5697F795489C8710BCC72141FF0AFA54@ex07.net.ucsf.edu> Message-ID: <1383921987.76409.YahooMailNeo@web163104.mail.bf1.yahoo.com> There is only a semantics difference. "Reticulum" is a connective mesh holding together some organs components such as liver, spleen, lymph nodes and bone marrow. In the German literature is called "glitterfassern" and many silver stains, such as Gomori, are specific for it. "Reticulin" is used either to refer to the "holding mesh" or to the stain, especially when dealing with bone marrow specimens. Ren? J. ________________________________ From: "Morken, Timothy" To: Histonet Sent: Thursday, November 7, 2013 6:02 PM Subject: [Histonet] Histo arcania ... Reticulum vs Reticulin Oh Great Histonet, how do you describe the difference, if any, between the terms "reticulum" and "reticulin." Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center Box 1656 505 Parnassus Ave San Francisco, CA 94143 USA 415.353.1266? (office) tim.morken@ucsfmedctr.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Taylor.Clifford <@t> va.gov Fri Nov 8 09:01:52 2013 From: Taylor.Clifford <@t> va.gov (Clifford, Taylor) Date: Fri Nov 8 09:03:06 2013 Subject: [Histonet] IHC Neuropathology Position Message-ID: <7FF33CF7105F23409F31E748D906B489A02678E678@R04BYNMSGB2.r04.med.va.gov> An academic research laboratory devoted to the study of hereditary ataxia and located at the VA Medical Center in Albany, N.Y., is seeking a third research associate. Technologies include Routine and Advanced Immunohistochemistry of tissue sections, Qualitative and Quantitative X-ray Fluorescence of embedded tissues, Western blotting, ELISA, PCR, Electrophoresis and other selected molecular techniques. Applicants should have a degree in biomedical science, neuropsychology, or histotechnology. Prior laboratory experience in neuroanatomy or neuroscience is preferred but not required. Send request for more information; resum?, electronic copies of transcripts and a list of three references to Dr. A Koeppen, principal investigator, arnulf.koeppen@med.va.gov Position is also listed on Monster - Neuropathology Research Associate If interested in applying, send all requested items from above!! Taylor CM Clifford Research Associate Albany Research Institute Albany Stratton VA Medical Center 113 Holland Avenue Albany, NY 12208 518-626-5664 Taylor.Clifford@va.gov From Taylor.Clifford <@t> va.gov Fri Nov 8 10:53:21 2013 From: Taylor.Clifford <@t> va.gov (Clifford, Taylor) Date: Fri Nov 8 10:53:56 2013 Subject: [Histonet] H&E Stainer Message-ID: <7FF33CF7105F23409F31E748D906B489A02678E679@R04BYNMSGB2.r04.med.va.gov> Looking for suggestions on an automatic H&E stainer for a research lab. We really only do H&E's on new cases every few weeks but are considering purchasing an automated stainer to save on technician time for the prep of IHC slides we currently do manually. We have rehydrating and dehydrating stations in separate hoods with a benchtop oven but having an automated stainer to rehydrate/dehydrate and possibly dry would be more efficient. Not looking for suggestions on automated IHC machines, we still prefer to do those protocols by hand. Potential machine would have to have the capability for multiple programs for H & E as well as prep for IHC slides - rehydration & methanol/H2O2 suppression etc., and it would be nice to have programming for dehydrating slides after IHC or special stains. From Allison.Scott <@t> harrishealth.org Fri Nov 8 10:58:30 2013 From: Allison.Scott <@t> harrishealth.org (Scott, Allison D) Date: Fri Nov 8 10:58:34 2013 Subject: [Histonet] Nail processing Message-ID: Happy Friday to all. I hope everyone has a great weekend. I am I need of a procedure for processing nails. We are having problems with keeping the nails on during gms special staining. We are using the ventana machine not by hand. I remember a procedure that used nair, it has been along time and I cant remember. Any help will be appreciated. Allison Scott HT(ASCP) Supervisor, Histology Lab LBJ Hospital Harris Health System Office: 713-566-2148 Lab: 713-566-5287 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. From doug.porter <@t> caplab.org Fri Nov 8 11:12:28 2013 From: doug.porter <@t> caplab.org (Douglas Porter) Date: Fri Nov 8 11:09:43 2013 Subject: [Histonet] Nail processing In-Reply-To: References: Message-ID: <001801cedca5$b480fd70$1d82f850$@caplab.org> 5% KOH for as long as need. The thicker the nail, the longer the time. But, if you leave it too long...forget it's in there...it will turn to mush. Happy Friday to you as well!! Whoop...Whoop!! Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.porter@caplab.org www.caplab.org ? ? The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, copying, forwarding or capture of this communication is strictly prohibited. If you have received this communication in error, please notify me immediately by return e-mail and delete this and all copies. Thank-you. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Friday, November 08, 2013 11:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Nail processing Happy Friday to all. I hope everyone has a great weekend. I am I need of a procedure for processing nails. We are having problems with keeping the nails on during gms special staining. We are using the ventana machine not by hand. I remember a procedure that used nair, it has been along time and I cant remember. Any help will be appreciated. Allison Scott HT(ASCP) Supervisor, Histology Lab LBJ Hospital Harris Health System Office: 713-566-2148 Lab: 713-566-5287 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wdesalvo.cac <@t> outlook.com Fri Nov 8 11:15:05 2013 From: wdesalvo.cac <@t> outlook.com (WILLIAM DESALVO) Date: Fri Nov 8 11:15:10 2013 Subject: [Histonet] Nail processing In-Reply-To: References: Message-ID: This process has worked for us for years. I strongly suggest the Fisher Brand Burnt Orange Plus Slides. We have tried them all and consistently the Burnt Orange work the best. We use them when we really need something to stick to the slide. Can't scientifically explain, just lots of testing and use. Grossing Sections for Histology:Complete 10% Formalin FixationNails are soaked in a 10% solution of potassium hydroxide.Hard nails may require 30-45 minutes. Softer nails should be soaked for 10-30 minutes.Over soaking can destroy the specimen.Rinse and place in Formalin to wait for processing.Submit one cassette of representative section(s). Microtomy of SectionsUse Fisher Brand Burnt Orange Plus Slides.Cut two sections per slide.Air Dry then place in 60? C oven for 2 hours.Allow to come to room temp before starting staining process. William DeSalvo, BS HTL(ASCP) Production Manager-Anatomic Pathology Chair, NSH Quality Management Committee Owner/Consultant, Collaborative Advantage Consulting > From: Allison.Scott@harrishealth.org > To: histonet@lists.utsouthwestern.edu > Date: Fri, 8 Nov 2013 16:58:30 +0000 > Subject: [Histonet] Nail processing > > Happy Friday to all. I hope everyone has a great weekend. I am I need of a procedure for processing nails. We are having problems with keeping the nails on during gms special staining. We are using the ventana machine not by hand. I remember a procedure that used nair, it has been along time and I cant remember. Any help will be appreciated. > > > > Allison Scott HT(ASCP) > Supervisor, Histology Lab > LBJ Hospital > Harris Health System > Office: 713-566-2148 > Lab: 713-566-5287 > > CONFIDENTIALITY NOTICE: > If you have received this e-mail in error, please immediately notify the > sender by return e-mail and delete this e-mail and any attachments from > your computer system. > > To the extent the information in this e-mail and any attachments contain > protected health information as defined by the Health Insurance Portability > and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and > 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or > privileged. This e-mail may also be confidential and/or privileged under > Texas law. The e-mail is for the use of only the individual or entity named > above. If you are not the intended recipient, or any authorized > representative of the intended recipient, you are hereby notified that any > review, dissemination or copying of this e-mail and its attachments is > strictly prohibited. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Fri Nov 8 11:52:56 2013 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Fri Nov 8 11:53:11 2013 Subject: [Histonet] Histo arcania ... Reticulum vs Reticulin In-Reply-To: <1383921987.76409.YahooMailNeo@web163104.mail.bf1.yahoo.com> References: <761E2B5697F795489C8710BCC72141FF0AFA54@ex07.net.ucsf.edu> <1383921987.76409.YahooMailNeo@web163104.mail.bf1.yahoo.com> Message-ID: <761E2B5697F795489C8710BCC72141FF0B0A97@ex07.net.ucsf.edu> Here is a bit more on the terms this article gives some background on Reticulin vs Reticulum http://en.wikipedia.org/wiki/Reticular_fiber Briefly, "Reticulin" refers specifically to collagen III. "Reticulum" is an old term that referred to generalized staining of "reticular fibers" and at that time they had no idea of the varieties of collagen. Since the silver stain has been shown to be non-specific the term "Reticulum" may be more correct for the generalized staining. Also see http://en.wikipedia.org/wiki/Reticulin http://en.wikipedia.org/wiki/Reticulin_stain Histochemistry. 1978 Sep 15;57(3):177-87. Silver impregnation methods for reticulum fibers and reticulin: a re-investigation of their origins and specificity. Puchtler H, Waldrop FW. Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: Friday, November 08, 2013 6:46 AM To: Morken, Timothy; Histonet Subject: Re: [Histonet] Histo arcania ... Reticulum vs Reticulin There is only a semantics difference. "Reticulum" is a connective mesh holding together some organs components such as liver, spleen, lymph nodes and bone marrow. In the German literature is called "glitterfassern" and many silver stains, such as Gomori, are specific for it. "Reticulin" is used either to refer to the "holding mesh" or to the stain, especially when dealing with bone marrow specimens. Ren? J. From: "Morken, Timothy" > To: Histonet > Sent: Thursday, November 7, 2013 6:02 PM Subject: [Histonet] Histo arcania ... Reticulum vs Reticulin Oh Great Histonet, how do you describe the difference, if any, between the terms "reticulum" and "reticulin." Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center Box 1656 505 Parnassus Ave San Francisco, CA 94143 USA 415.353.1266 (office) tim.morken@ucsfmedctr.org> _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Nov 8 12:11:27 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Nov 8 12:11:34 2013 Subject: [Histonet] Nail processing In-Reply-To: References: Message-ID: <1383934287.59276.YahooMailNeo@web163104.mail.bf1.yahoo.com> Fix the nails as usual. Place them in a 10% aq. sol. of either sodium or potassium hydroxyde for 30 minutes. Wash well and process as usual. The sections will stay in the slides but if you want another "layer of security" ad Elmer's glue to the slides and let it dry.?"Fish" the sections from the water bath with this slides so treated. Ren? J.? ________________________________ From: "Scott, Allison D" To: "histonet@lists.utsouthwestern.edu" Sent: Friday, November 8, 2013 11:58 AM Subject: [Histonet] Nail processing Happy Friday to all.? I hope everyone has a great weekend.? I am I need of a procedure for processing nails.? We are having problems with keeping the nails on during gms special staining.? We are using the ventana machine not by hand.? I remember a procedure that used nair, it has been along time and I cant remember.? Any help will be appreciated. Allison Scott HT(ASCP) Supervisor, Histology Lab LBJ Hospital Harris Health System Office: 713-566-2148 Lab: 713-566-5287 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged.? This e-mail may also be confidential and/or privileged under Texas law.? The e-mail is for the use of only the individual or entity named above.? If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From anolan <@t> prometheushealthcare.com Fri Nov 8 12:29:24 2013 From: anolan <@t> prometheushealthcare.com (anolan@prometheushealthcare.com) Date: Fri Nov 8 12:28:25 2013 Subject: [Histonet] Histology Opportunities Message-ID: <017701cedcb0$74b86740$5e2935c0$@prometheushealthcare.com> Hi all, I have some new histology opportunities in NYC. Looking for candidates with a NY State license and a Bachelor's Degree. Also, I am still looking for an IHC Manager for a laboratory in San Francisco. ASCP required. If you'd like to apply please contact me directly. Thanks! Anna Nolan - Recruiter Prometheus Healthcare Direct Line 301-693-8908 Office 301-693-9057 Fax 301-368-2478 anolan @prometheushealthcare.com http://www.linkedin.com/pub/annelise-nolan/55/ba0/ab6 www.prometheushealthcare.com From gu.lang <@t> gmx.at Sat Nov 9 02:12:56 2013 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sat Nov 9 02:13:14 2013 Subject: AW: [Histonet] Histo arcania ... Reticulum vs Reticulin In-Reply-To: <1383921987.76409.YahooMailNeo@web163104.mail.bf1.yahoo.com> References: <761E2B5697F795489C8710BCC72141FF0AFA54@ex07.net.ucsf.edu> <1383921987.76409.YahooMailNeo@web163104.mail.bf1.yahoo.com> Message-ID: <000301cedd23$7fd34870$7f79d950$@gmx.at> A small correction: The German word is not "Glitterfaser" but "Gitterfaser". And "Gitter" means net or grid. My old latin dictionary says: Reticulum means "small net" , going back to "rete" as net. I think the correct name for the stain should be reticulum-stain, because it's more or less unspecific to special proteins. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Rene J Buesa Gesendet: Freitag, 08. November 2013 15:46 An: Morken, Timothy; Histonet Betreff: Re: [Histonet] Histo arcania ... Reticulum vs Reticulin There is only a semantics difference. "Reticulum" is a connective mesh holding together some organs components such as liver, spleen, lymph nodes and bone marrow. In the German literature is called "glitterfassern" and many silver stains, such as Gomori, are specific for it. "Reticulin" is used either to refer to the "holding mesh" or to the stain, especially when dealing with bone marrow specimens. Ren? J. ________________________________ From: "Morken, Timothy" To: Histonet Sent: Thursday, November 7, 2013 6:02 PM Subject: [Histonet] Histo arcania ... Reticulum vs Reticulin Oh Great Histonet, how do you describe the difference, if any, between the terms "reticulum" and "reticulin." Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center Box 1656 505 Parnassus Ave San Francisco, CA 94143 USA 415.353.1266? (office) tim.morken@ucsfmedctr.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From trathborne <@t> somerset-healthcare.com Sat Nov 9 08:24:38 2013 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Sat Nov 9 08:25:15 2013 Subject: [Histonet] RE: H&E Stainer In-Reply-To: <7FF33CF7105F23409F31E748D906B489A02678E679@R04BYNMSGB2.r04.med.va.gov> References: <7FF33CF7105F23409F31E748D906B489A02678E679@R04BYNMSGB2.r04.med.va.gov> Message-ID: <3AD061FE740D464FAC7BF6B5CFB75707A8B2E0F4@smcmail02.somerset-healthcare.com> We have the Leica ST5020, which has the capability to store multiple programs (we have over 10). It has a total of 40 stations, of which 4 are ovens, and another 4 are washes. We also have the (optional) CV5030 which is the coverslipper which attaches to it. While the stainer is not the quickest on the market, it should be perfect for what you're looking for. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Clifford, Taylor Sent: Friday, November 08, 2013 11:53 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] H&E Stainer Looking for suggestions on an automatic H&E stainer for a research lab. We really only do H&E's on new cases every few weeks but are considering purchasing an automated stainer to save on technician time for the prep of IHC slides we currently do manually. We have rehydrating and dehydrating stations in separate hoods with a benchtop oven but having an automated stainer to rehydrate/dehydrate and possibly dry would be more efficient. Not looking for suggestions on automated IHC machines, we still prefer to do those protocols by hand. Potential machine would have to have the capability for multiple programs for H & E as well as prep for IHC slides - rehydration & methanol/H2O2 suppression etc., and it would be nice to have programming for dehydrating slides after IHC or special stains. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From chesarato <@t> hotmail.com Sat Nov 9 13:02:19 2013 From: chesarato <@t> hotmail.com (Cesar Francisco Romero) Date: Sat Nov 9 13:02:24 2013 Subject: [Histonet] RE: Reticulum Vs Reticulin Message-ID: Reticulum means Net ( Red in Spanish or Web in English ). It refers to the Net that exist in Lymphoid tissue of any kind ( Payer Patches ? Lymph Nodes ? Spleen ). Dendritic Cells attach to this Net, that?s why in the past it was used the term Reticulum Cells Sarcoma ( for Dendritic Cells Sarcoma ). Reticulin ( Reticulina in Spanish ) is the Silver Technic for Staining this Type III Collagen Fibers of the Net. From lpwenk <@t> sbcglobal.net Sun Nov 10 05:18:46 2013 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Sun Nov 10 05:18:52 2013 Subject: [Histonet] 2013 ASCP Wage Survey Message-ID: It?s HERE! Access through either NSH www.nsh.org Link is on left side, about half way down. or ASCP www.ascp.org click on survey on bottom middle at the bottom of the article, click on where is says ?access here? For an easier reading PDF of the full text, click on PDF just to the right of the article. Peggy A. Wenk, HTL(ASCP)SLS From glenn.m.manning <@t> thermofisher.com Sun Nov 10 15:31:00 2013 From: glenn.m.manning <@t> thermofisher.com (Manning, Glenn M.) Date: Sun Nov 10 15:31:06 2013 Subject: [Histonet] Re: Histonet Digest, Vol 120, Issue 13 In-Reply-To: <20131110180246.41DA91C216@ukmx04.thermofisher.com> Message-ID: <4EC1AA67AC43444591588346A272F9001D2AD760C7@USPHO-MXVS02.amer.thermo.com> An to be on in between sales calls Glenn Manning Sales Manager, Anatomical Pathology Thermo Fisher Scientific +61 478 487 416 Sent from my Blackberry ----- Original Message ----- From: histonet-request@lists.utsouthwestern.edu [mailto:histonet-request@lists.utsouthwestern.edu] Sent: Sunday, November 10, 2013 11:02 AM US Mountain Standard Time To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 120, Issue 13 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: Reticulum Vs Reticulin (Cesar Francisco Romero) 2. 2013 ASCP Wage Survey (Lee & Peggy Wenk) ---------------------------------------------------------------------- Message: 1 Date: Sat, 9 Nov 2013 16:02:19 -0300 From: Cesar Francisco Romero Subject: [Histonet] RE: Reticulum Vs Reticulin To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="Windows-1252" Reticulum means Net ( Red in Spanish or Web in English ). It refers to the Net that exist in Lymphoid tissue of any kind ( Payer Patches ? Lymph Nodes ? Spleen ). Dendritic Cells attach to this Net, that?s why in the past it was used the term Reticulum Cells Sarcoma ( for Dendritic Cells Sarcoma ). Reticulin ( Reticulina in Spanish ) is the Silver Technic for Staining this Type III Collagen Fibers of the Net. ------------------------------ Message: 2 Date: Sun, 10 Nov 2013 06:18:46 -0500 From: "Lee & Peggy Wenk" Subject: [Histonet] 2013 ASCP Wage Survey To: "Histonet" Message-ID: Content-Type: text/plain; charset="utf-8" It???s HERE! Access through either NSH www.nsh.org Link is on left side, about half way down. or ASCP www.ascp.org click on survey on bottom middle at the bottom of the article, click on where is says ???access here??? For an easier reading PDF of the full text, click on PDF just to the right of the article. Peggy A. Wenk, HTL(ASCP)SLS ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 120, Issue 13 ***************************************** Worldwide Confidentiality Note: Dissemination, distribution or copying of this email or the information herein by anyone other than the intended recipient, or an employee or agent of a system responsible for delivering the message to the intended recipient, is prohibited. If you are not the intended recipient, please inform the sender and delete all copies. The company does not accept credit card information via fax or email. To process a payment please contact our customer service department in either Australia (1300 735 292) or New Zealand (0800 933 966). From glenn.m.manning <@t> thermofisher.com Sun Nov 10 17:32:42 2013 From: glenn.m.manning <@t> thermofisher.com (Manning, Glenn M.) Date: Sun Nov 10 17:32:49 2013 Subject: [Histonet] Recall: Histonet Digest, Vol 120, Issue 13 Message-ID: <4EC1AA67AC43444591588346A272F9001D2B0005C9@USPHO-MXVS02.amer.thermo.com> Manning, Glenn M. would like to recall the message, "Histonet Digest, Vol 120, Issue 13". Worldwide Confidentiality Note: Dissemination, distribution or copying of this email or the information herein by anyone other than the intended recipient, or an employee or agent of a system responsible for delivering the message to the intended recipient, is prohibited. If you are not the intended recipient, please inform the sender and delete all copies. The company does not accept credit card information via fax or email. To process a payment please contact our customer service department in either Australia (1300 735 292) or New Zealand (0800 933 966). From dhenders01 <@t> msn.com Sun Nov 10 17:56:14 2013 From: dhenders01 <@t> msn.com (DAVID HENDERSON) Date: Sun Nov 10 17:56:18 2013 Subject: [Histonet] Frozen IF Specimen Storage: Is -80 C really necessary; will -20 C work? Message-ID: We routinely store our frozen IF specimens (renal and skin biopsies) embedded in OCT and sealed in plastic bags for at least 4 yrs in a -80C freezer. Cases that are positively diagnosed by the pathologist, are used as positive controls for subsequent IF staining. In practice, most positive controls are used within 1-2 years of their original storage. Our -80C freezer has died, and we are temporarily storing in a different -80C freezer in another building on campus. My supervisor asked me to research whether or not a -20C freezer will suffice for our storage needs, or must we anti up for the much more costly -80C replacement or repair. I would appreciate receiving suggestions backed by experience, or educated thoughts about the advisability of -20C storage of specimens for future IF staining. Thanks, David Henderson, HTL RML, Tulsa, OK From Kathy.Machado <@t> LPNT.net Mon Nov 11 08:13:10 2013 From: Kathy.Machado <@t> LPNT.net (Kathy.Machado@LPNT.net) Date: Mon Nov 11 08:13:16 2013 Subject: [Histonet] H&E stainer Message-ID: I have a Leica autostainer that is capable of several different programs, including dehydrating/rehydrating. I use it to counterstain my IHC so the hematoxylin does not mess up my IHC stainer. I like this stainer because it is easy to use and maintain. Kathy Machado,HTL Danville Regional Medical Center From bellopaz19 <@t> yahoo.es Mon Nov 11 08:37:20 2013 From: bellopaz19 <@t> yahoo.es (Yoanna Bello Arredondo) Date: Mon Nov 11 08:37:25 2013 Subject: [Histonet] PicroSirius Red in Frozen Sections Message-ID: <1384180640.41003.YahooMailNeo@web28906.mail.ir2.yahoo.com> Hello! I did a Picrosirius red stain using the polysciences kit in paraffin section cut at 4microns. The stain looks great. Later on my boss asked me to do the picro using the same kit but in frozen section. The stain looks very, very intense. Sections are really red. When looking under the brightfield microscope there is not yellow, the entire tissue is red. Under the polarized microscope, we can see the fiber but no? like the paraffin sections. Does anybody have come across this situation before? Does the picrosirius stain only works for paraffin sections? I will appreciate any feedback on the matter. I have been searching online for a protocol of Picrosirius stain for frozens, but no luck. Thank you in advance, Yoanna Bello From Kathy.Machado <@t> LPNT.net Mon Nov 11 09:39:32 2013 From: Kathy.Machado <@t> LPNT.net (Kathy.Machado@LPNT.net) Date: Mon Nov 11 09:40:43 2013 Subject: [Histonet] Leica vs Ventana Message-ID: Hi Mehndi. I hope this is still useful information. I wanted to let you know that I am a faithful Ventana user. I have been using strictly Ventana IHC stainers for several years now. Currently we have two Ultras. They are very user friendly and the tech support is awesome. We looked into a Bond before switching from DAKO and found the Ventana is much easier to understand as far as the constant feed goes. Both of the Ultras run all day long and I am able to put on and take off slides easily. Hope this helps! If you have further questions please feel free to contact me. Kathy Machado Danville Regional Medical Center 434-799-3867 Kathy.machado@lpnt.net From rjbuesa <@t> yahoo.com Mon Nov 11 10:09:02 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Nov 11 10:09:07 2013 Subject: [Histonet] Frozen IF Specimen Storage: Is -80 C really necessary; will -20 C work? In-Reply-To: References: Message-ID: <1384186142.72388.YahooMailNeo@web163101.mail.bf1.yahoo.com> I really do not know of any study comparing -20?C vs -80?C or if this -80?C is the result of a commercially driven? "ideal" storage method. I advise you to check about any bibliographic references BUT meanwhile I also advise you to keep your precious and irreplaceable material at -80?C. I do not think that you should risk losing all your samples just to work "on the cheap". I always kept my tumor bank and IF samples at -80?C and when our freezer also died year ago, I was lucky enough to get the approval to buy a new one. ?Do not rush to save money Ren? J. ________________________________ From: DAVID HENDERSON To: "histonet@lists.utsouthwestern.edu" Sent: Sunday, November 10, 2013 6:56 PM Subject: [Histonet] Frozen IF Specimen Storage: Is -80 C really necessary; will -20 C work? We routinely store our frozen IF specimens (renal and skin biopsies) embedded in OCT and sealed in plastic bags for at least 4 yrs in a -80C freezer. Cases that are positively diagnosed by the pathologist, are used as positive controls for subsequent IF staining. In practice, most positive controls are used within 1-2 years of their original storage. Our -80C freezer has died, and we are temporarily storing in a different -80C freezer in another building on campus. My supervisor asked me to research whether or not a -20C freezer will suffice for our storage needs, or must we anti up for the much more costly -80C replacement or repair. I would appreciate receiving suggestions backed by experience, or educated thoughts about the advisability of -20C storage of specimens for future IF staining. Thanks, David Henderson, HTL RML, Tulsa, OK _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sngastro <@t> sbcglobal.net Mon Nov 11 12:48:58 2013 From: sngastro <@t> sbcglobal.net (Janice Paquin) Date: Mon Nov 11 12:52:48 2013 Subject: [Histonet] FT Histotech Job Message-ID: FT Histotech fo a GI Path Lab in Northern California Great pay plus benefits Must be license/certified Experience of at least 2 years Embedding/special stains/processing Self motivator From cathy.crumpton <@t> tuality.org Mon Nov 11 13:37:29 2013 From: cathy.crumpton <@t> tuality.org (Cathy Crumpton) Date: Mon Nov 11 13:37:35 2013 Subject: [Histonet] Setting up ASR antibody to meet CAP requirements Message-ID: For the first time my pathologist would like to bring an ASR antibody in house. It is made by Biocare, and our validation slides look good compared to the other clone we want to get rid of. However, I see in question 12425 of my checklist that I have to "establish or verify the performance characteristics... in accordance with the Method Performance Specifications section of the All Common Checklist." Is there anyone who would be willing to share their procedures on verifying and validating ASR's for IHC purposes? Cathy Crumpton HT(ASCP), Lead Histotechnician Tuality Community Hospital 503-681-1292 From m_chadwell <@t> hotmail.com Mon Nov 11 13:46:35 2013 From: m_chadwell <@t> hotmail.com (margaret chadwell) Date: Mon Nov 11 13:55:51 2013 Subject: =?utf-8?Q?Re:_[Histonet]_Frozen_IF_Specimen_Storage:_Is_-80_C_really_nece?= =?utf-8?Q?ssary;=09will_-20_C_work=3F?= Message-ID: Hi David, An institute that I work for would take the samples out of the -80 and place them into a -20 before I cut them, sometimes this would be overnight other times several hours. We were experiencing freeze thaw artifact on those samples, when asked why they were doing this, they thought this was the recommended protocol. Once they stopped the practice, and left the samples in the -80 till cutting, no freeze thaw artifact was encountered again. As a result of this, I would recommend you don?t place them in the -20. I also only remove the samples I want to cut at the moment and don't place many samples in the cryostat at one time. Margie Chadwell Sent from Windows Mail From: Rene J Buesa Sent: ?Monday?, ?November? ?11?, ?2013 ?8?:?09? ?AM To: DAVID HENDERSON, histonet@lists.utsouthwestern.edu I really do not know of any study comparing -20?C vs -80?C or if this -80?C is the result of a commercially driven "ideal" storage method. I advise you to check about any bibliographic references BUT meanwhile I also advise you to keep your precious and irreplaceable material at -80?C. I do not think that you should risk losing all your samples just to work "on the cheap". I always kept my tumor bank and IF samples at -80?C and when our freezer also died year ago, I was lucky enough to get the approval to buy a new one. Do not rush to save money Ren? J. ________________________________ From: DAVID HENDERSON To: "histonet@lists.utsouthwestern.edu" Sent: Sunday, November 10, 2013 6:56 PM Subject: [Histonet] Frozen IF Specimen Storage: Is -80 C really necessary; will -20 C work? We routinely store our frozen IF specimens (renal and skin biopsies) embedded in OCT and sealed in plastic bags for at least 4 yrs in a -80C freezer. Cases that are positively diagnosed by the pathologist, are used as positive controls for subsequent IF staining. In practice, most positive controls are used within 1-2 years of their original storage. Our -80C freezer has died, and we are temporarily storing in a different -80C freezer in another building on campus. My supervisor asked me to research whether or not a -20C freezer will suffice for our storage needs, or must we anti up for the much more costly -80C replacement or repair. I would appreciate receiving suggestions backed by experience, or educated thoughts about the advisability of -20C storage of specimens for future IF staining. Thanks, David Henderson, HTL RML, Tulsa, OK _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kblack <@t> reagan.com Mon Nov 11 15:38:58 2013 From: kblack <@t> reagan.com (Konni Black) Date: Mon Nov 11 15:39:01 2013 Subject: [Histonet] Immediate Open Position in Gig Harbor, WA Message-ID: <1384205938.042931276@webmail.reagan.com> Hi All, A Dermatologist in Gig Harbor, Washington is looking for a full-time HT or HTL. Candidates must meet CLIA compliance requirements to perform gross. Ability to perform Mohs is a plus but in-house Mohs training is available. Anyone interested in more information may reply to this e-mail. Attached resumes are appreciated. Thank you, Konni Black From plantation30 <@t> msn.com Mon Nov 11 16:12:51 2013 From: plantation30 <@t> msn.com (Phyllis Burnette) Date: Mon Nov 11 16:12:55 2013 Subject: [Histonet] Leica vs Ventana Message-ID: Our hospital facility, as well, are faithful Ventana customers. We have 3 ultras and appreciate the ease and flexibility needed for a busy hospital facility. The slide drawers are random access so the slides are staining independently from one another. Another great feature of the ultra is the random access points where antibodies, exhausted reagents, etc. can be added once the run starts and at multiple times during the staining run. Our laboratory has found that the quality of the IHC stains are exceptional and consistent from lot to lot. The sales staff and technical support is superior and the engineers that work on our equipment are very thorough. Of course, Ventana is light-years above with specimen tracking for the histology laboratory and we are very excited that with this addition, all slides will be tracked from start to finish. Can't say enough, we really love this product!!! Phyllis S. Burnette HT, ASCP Anatomicals Supervisor Riverside Regional Medical Center 757-594-2885 From tony.henwood <@t> health.nsw.gov.au Mon Nov 11 16:43:31 2013 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Mon Nov 11 16:43:50 2013 Subject: [Histonet] Frozen IF Specimen Storage: Is -80 C really necessary; will -20 C work? In-Reply-To: <1384186142.72388.YahooMailNeo@web163101.mail.bf1.yahoo.com> References: <1384186142.72388.YahooMailNeo@web163101.mail.bf1.yahoo.com> Message-ID: <6D6BD1DE8A5571489398B392A38A71579D470AAE@xmdb04.nch.kids> The following (from "The Book" - that may never be published!): It is also important to remember that -20oC is not suitable for storing frozen tissue. Have you ever noticed that meat stored too long in the freezer suffers "freezer-burn"; it appears dried-out? The condition is primarily caused by sublimation. Water evaporates at all temperatures, even from the surface of solid ice. If air adjacent to ice is cold enough (so the ice won't melt) and the air is dry enough, water molecules go directly from solid phase (ice) to gaseous phase (vapour) without going through a liquid phase. When the constantly vibrating water molecules in foods stored in a freezer migrate to the surface, crystals of ice outside of the solid food are formed, and some water molecules escape into the air (by sublimation). The meat parts now deprived of moisture become dry and shrivelled and look burnt. In meats, air can cause fats to oxidize. Frost-free freezers are the result of having a fan and air circulation inside the freezer. The sub-zero temperature combined with the air circulation that keeps the air arid significantly accelerates the sublimation process. This keeps freezer walls and shelves free of ice, although ice-cubes will continually sublimate. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, 12 November 2013 3:09 AM To: DAVID HENDERSON; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Frozen IF Specimen Storage: Is -80 C really necessary; will -20 C work? I really do not know of any study comparing -20?C vs -80?C or if this -80?C is the result of a commercially driven? "ideal" storage method. I advise you to check about any bibliographic references BUT meanwhile I also advise you to keep your precious and irreplaceable material at -80?C. I do not think that you should risk losing all your samples just to work "on the cheap". I always kept my tumor bank and IF samples at -80?C and when our freezer also died year ago, I was lucky enough to get the approval to buy a new one. ?Do not rush to save money Ren? J. ________________________________ From: DAVID HENDERSON To: "histonet@lists.utsouthwestern.edu" Sent: Sunday, November 10, 2013 6:56 PM Subject: [Histonet] Frozen IF Specimen Storage: Is -80 C really necessary; will -20 C work? We routinely store our frozen IF specimens (renal and skin biopsies) embedded in OCT and sealed in plastic bags for at least 4 yrs in a -80C freezer. Cases that are positively diagnosed by the pathologist, are used as positive controls for subsequent IF staining. In practice, most positive controls are used within 1-2 years of their original storage. Our -80C freezer has died, and we are temporarily storing in a different -80C freezer in another building on campus. My supervisor asked me to research whether or not a -20C freezer will suffice for our storage needs, or must we anti up for the much more costly -80C replacement or repair. I would appreciate receiving suggestions backed by experience, or educated thoughts about the advisability of -20C storage of specimens for future IF staining. Thanks, David Henderson, HTL RML, Tulsa, OK _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From lpwenk <@t> sbcglobal.net Mon Nov 11 18:34:32 2013 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Mon Nov 11 18:34:37 2013 Subject: [Histonet] PicroSirius Red in Frozen Sections In-Reply-To: <1384180640.41003.YahooMailNeo@web28906.mail.ir2.yahoo.com> References: <1384180640.41003.YahooMailNeo@web28906.mail.ir2.yahoo.com> Message-ID: <2ABC0BE3B30C4B8091C82B0CC2CA35F1@HP2010> I'm going to try to take a stab at this, but I don't know specifically about the picrosirius red stain. I'm going to talk in general about stains and fixation (or lack thereof in the frozen section (FS)), and the relationship between fixation and staining. I'm going to start out very simple, and then get a little more complicated, so hang in there. (If you are not interested in dye theory, delete now, as this is going to get long. But if you like dye theory (like I do), I hope you enjoy this. And if you do like dye theory, let me know if you think I'm correct, or if I?m barking up the wrong tree.) Stains bind to proteins in the tissue, which are made up of amino acids, which either are positively charged, negatively charged, or non-polar (no charge). Dyes usually have positive ions and negative ions, but tend to have more of one type, and thus are considered either positively charged or negatively charged, and/or tend to bind via one type of bonding over another (e.g., hydrogen bonds vs. covalent). Different Proteins in tissue have their own unique shape and density. Dyes have their own shapes. The two shapes have to be somewhat similar for the proteins to bind to a dye. A round ball might be able to fit into an open space in a protein, whereas a stiff long dye (think something the shape of a ruler) might not be able to fit into that space. Differently Proteins have their own density (loose to tightly packed). Dyes have their own sizes (small to very large). The dye has to be able to fit into the protein, so a large dye may find it difficult to fit into a dense bunch of protein, whereas a small dye can fit into loose protein and dense protein. Therefore, for a dye to bind to a protein, the charged ions on the dyes have to be the opposite of the charges on the amino acids/proteins (positive binding to negative). And the charges on the dyes have to line up with the charges on the proteins, so the dye has to be able to "fit" into the protein, and the charges have to line up. Therefore, if we do something that changes the CHARGES on the protein, and/or the SHAPE of the proteins, and/or the DENSITY of the proteins, the dye may bind differently (not at all, very little, or too much). (Conversely, changing the dye in any way could cause different staining patterns, but since you said it was the same kit, and since you said "later" your boss asked for the stain to be done on a FS, I'm expecting it to have been the same day or the next, so I don't think the kit went bad, and I'm assuming you did the stain correctly. So I won't be discussing bad staining due to bad dyes or performing the stain incorrectly.) Now, onto fixation vs. frozen section (FS). I'm assuming 10% formalin was the fixative, or a zinc formalin, or a formalin substitute (glyoxal). It's the formalin/formaldehyde/glyoxal that is negatively charged. It will bind with positive amino acids in the protein. Let's assume there were 10 + and 10 - amino acids on the protein, so the overall charge of the protein is a net zero. Let's bind 4 of the + amino acids with - charged formalin. You now have 6 + and 10 - amino acids, so you have more negative amino acids than positive, so your tissue is more negatively charged. You have just changed the CHARGES on the proteins. Fixatives cross-link proteins, and pull them in different directions. You have therefore changed the SHAPE of the protein. Since the fixative is cross-linking the protein, and pulling the proteins in different directions, some proteins are going to be pulled further apart, thus becoming looser in density, while other proteins are being pulled closer together, or being made denser. You have therefore changed the DENSITY of the protein. Most dyes/stains used in histology were designed to be used with formalin fixed tissue, and are therefore made to work with proteins that have had their charges changed, their shapes altered, and their density changed, according to the changes made by formalin. Frozen sections have NOT have any fixative, and are therefore similar to the unfixed tissue, without the changes in charged, shape, and density. So it should make sense that unfixed/FS tissues should/could stain differently than fixed tissue. Now, for sirius red specifically. There are several different sirius red dye molecules. I don't know which one in particular you used, but in this explanation, it doesn't really matter, because they all belong to the polyazo dye family. That means they are made up of several benzene rings (5 to 8), held together in a long row (linear - like the ruler I mentioned earlier) with azo bonds (Nitrogen double bonded to Nitrogen -N=N- which have hydrogens bonded to them, giving these bonds a positive charge), and several sulfonic acid groups bound to the benzene rings ( -SO3 ions, which are negatively charged). To me, these sirius red dye molecules look very similar to Congo red dye molecules, and according to Conn's Biological Stains, sirius red binds in a similar manner to Congo red. Therefore, these long linear dye molecules have to bind via hydrogen bonds (positive charges) to very specifically spaced negative charges (usually hydroxyl ions -OH) on the connective tissue. When these dyes bind, they do so in a way that the dyes are now parallel to another | | | | | | . This makes a crystal-like arrangement, and these dyes/connective tissue (with sirius red) or dyes/amyloid (with Congo red) will birefringe with polarizing microscopes. These specifically spaced hydrogen bonds between the dye and the protein will line up on FORMALIN FIXED tissue, where the connective tissue (or amyloid) have been cross-linked to make a specific shape, specific density, and the correct number of hydroxyl ions are in the correct position. However, in the case of FS, the protein shape, density, and number and position of hydroxyl ions are different than on formalin fixed tissue. In the case of your sirius red, since there are more negative amino acids on FS than on formalin fixed (in relation to the number of available positively charged amino acids), it might explained why the tissue looks redder (more hydrogen bonding of sirius red to negatively charged amino acids). However, the shape of the protein on FS is different than on the formalin fixed tissue, so the dye may not be binding | | | | |, but may be binding | \ / _ _ _ | /_. So, even though there are more sirius red dye molecules binding, they are not doing so in a parallel crystalline fashion. Therefore, there will not be polarization of the dye molecule. Does this long-winded explanation make sense? Now, if the problem is just overstaining, then more differentiation would help. But if the problem is the shape of the connective tissue proteins and the spacing of the hydroxyl ions, then nothing will help. However, like I said, I don't really know this stain, so I don't know if it really SHOULD work on FS or not. I'm just trying to explain why it may not be working. I know that with Congo red, for us, the staining actually seems better on FS than on formalin fixed, paraffin embedded tissue. But we do get more connective tissue picking up Congo red on FS. So that can be a problem for us. Peggy A. Wenk, HTL(ASCP)SLS -----Original Message----- From: Yoanna Bello Arredondo Sent: Monday, November 11, 2013 9:37 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] PicroSirius Red in Frozen Sections Hello! I did a Picrosirius red stain using the polysciences kit in paraffin section cut at 4microns. The stain looks great. Later on my boss asked me to do the picro using the same kit but in frozen section. The stain looks very, very intense. Sections are really red. When looking under the brightfield microscope there is not yellow, the entire tissue is red. Under the polarized microscope, we can see the fiber but no like the paraffin sections. Does anybody have come across this situation before? Does the picrosirius stain only works for paraffin sections? I will appreciate any feedback on the matter. I have been searching online for a protocol of Picrosirius stain for frozens, but no luck. Thank you in advance, Yoanna Bello _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Mon Nov 11 20:46:00 2013 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Mon Nov 11 20:46:16 2013 Subject: [Histonet] Supervisor position, Histology, UC San Francisco Medical Center Message-ID: <761E2B5697F795489C8710BCC72141FF0B1416@ex07.net.ucsf.edu> The Histology Supervisor position has opened at UC San Francisco Medical Center in San Francisco, CA Some basic info: Supervises Histology, special stains and IHC. 13 histotechs 30 pathologists, 25 residents, 3 certified PA's, 6 histotech PA's Average 500 blocks/day, range is 300 to 900 Recuts = average 250 per day, up to 500 IHC = 300 slides per day, 230 antibodies on the menu 50 special stains per day 2 peloris processors, 3 VIP5 3 sakura embedding centers 9 Leica 2255 microtomes 4 Leica Bond IHC stainers, 1 Ventana Ultra Cerner Copath Plus LIS, version 2012, upgrading to version 2013 in the spring. Implementing Copath AB&T barcoding at this time. Go-live is in February. Please apply through the Jobs website: https://careers.ucsfmedicalcenter.org:8443/psp/hcmprd_cg/EMPLOYEE/HRMS/c/HRS_HRAM.HRS_CE.GBL?Page=HRS_CE_JOB_DTL&Action=A&SiteId=1&JobOpeningId=5546&PostingSeq=2 You can send a resume to me at tim.morken@ucsfmedctr.org so we can be sure HR passes your application on to us. If you have any questions, ask me. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center 505 Parnassus Ave, Box 1656 Room S570 San Francisco, CA 94143 (415) 353-1266 (ph) (415) 514-3403 (fax) tim.morken@ucsfmedctr.org Job Description Job ID: 5546 Job Title: HISTOLOGY AND IPOX SUPERVISOR Job Code: 7902 Department: Pathology-Surgical / Histology Location: Parnassus Full/Part Time: Full-Time Appointment Type: Career Shift: Variable Variable Weekly Hours: 40 ; 100% Union Information: This classification is not represented by a union Our legacy of unsurpassed patient care and unceasing mission to integrate high-tech medical research with clinical operations has led to our prestigious standing as one of the top 10 hospitals in the nation according to U.S. News & World Report. As a premier health care institution dedicated to advancing health worldwide, UCSF Medical Center can also be the best place to advance and shape your career. The medical center's employees are one of the most important reasons why we are recognized as one of the nation's best hospitals. To work at UCSF Medical Center is to be part of an institution that provides the highest caliber of care to patients; a nurturing, dynamic and team-oriented atmosphere in which to best use your skills and talents. Job Summary Under general direction from the Medical Director, the incumbent serves as supervisor to 3 Lead Histotechnologists and 9 Histotechnologists. In addition to supervisory duties, the incumbent serves as a technical expert providing direction to staff as well as performing all technical aspects of surgical histology and immunohistochemistry procedures. As supervisor: recruits, hires, trains, completes performance evaluations, and resolves employee issues. Provides orientation, completes competency assessments, maintains staff schedules, training and compliance documentation. Implements new tests and procedures as requested by Medical Directors. Updates and maintains the lab manuals and annual reviews required for accreditation. Integrates new technologies into the laboratory to include analyzing and selecting of new equipment that meets space constraints and operational needs. Ensures that new equipment and associated validation and staff training are performed. Ensures that all equipment is well-maintained. Ensures that staff training and documentation are completed as required. Ensures that QC documentation is complete and quality standards are maintained in the laboratory. Advises and assists researchers planning research projects and determines the ability of the laboratory to accommodate research projects, updating the Medical Director as required. Other duties as assigned. Required Qualifications * College degree in a biological science, chemistry or a related field or equivalent education, Work experience experience as a histotechnologist in a comparable high-volume hospital histology laboratory within the last seven years, including senior-level experience. * Demonstrated high-volume, high-quality sectioning and staining skills. * Ability to organize and prioritize responsibilities and perform well under pressure to meet deadlines. * Excellent interpersonal and communication skills. * ASCP certification: HT licensed or eligible required (candidate will be expected to obtain certification within 1 year), HTL desirable. * Demonstrated ability to organize and prioritize responsibilities and perform well under pressure to meet deadlines required. * Previous, recent supervisory experience in a Histology and /or Immunoperoxidase Laboratory required. * Must be experienced with information technology systems and the use of hardware and software in business and laboratory settings. * Must be an effective user of spreadsheets and database software. Preferred Qualifications * ASCP certification * HT or HTL-licensed strongly preferred. * Previous leadership experience in a hospital laboratory. Licensure/Certification N/A Equal Employment Opportunity UCSF is an Equal Opportunity/Affirmative Action Employer. All qualified applicants are encouraged to apply. Further information about the University of California, San Francisco, is available at diversity.ucsf.edu. UCSF seeks candidates whose skills, and personal and professional experience, have prepared them to contribute to our commitment to diversity and excellence, and the communities we serve. From avogaro <@t> science.unitn.it Tue Nov 12 02:01:37 2013 From: avogaro <@t> science.unitn.it (Laura Avogaro) Date: Tue Nov 12 02:01:47 2013 Subject: [Histonet] PicroSirius Red in Frozen Sections In-Reply-To: <1384180640.41003.YahooMailNeo@web28906.mail.ir2.yahoo.com> References: <1384180640.41003.YahooMailNeo@web28906.mail.ir2.yahoo.com> Message-ID: <2193.192.168.178.78.1384243297.squirrel@www.science.unitn.it> Dear Yoanna I performed Picrosirius Red staining on my tissue frozen sections with good results so I suggest you operate as follows: 1)Fix slide in 4% PFA (5 min); 2)Rinse in ddH2O; 3)Incubate in Phosphomolybdic Acid (2 minutes); 4)Rinse in ddH2O; 5)Incubate in Picrosirius Red for (1 h); 6)Wash in acidified water (2X, 1 min each); 7)Rinse in 70% EtOH (30?); 8)Dehydrate through ascending grades of alchol(80%, 90%, 100%); 9)Wash on Xilene (2X); 10)Mounting. Good luck and let me know! Bye Laura > Hello! > > I did a Picrosirius red stain using the polysciences kit in paraffin section cut at 4microns. The stain looks great. Later on my boss asked me > to do the picro using the same kit but in frozen section. The stain looks > very, very intense. Sections are really red. When looking under the brightfield microscope there is not yellow, the entire tissue is red. Under the polarized microscope, we can see the fiber but no? like the paraffin sections. Does anybody have come across this situation before? Does the picrosirius stain only works for paraffin sections? I will appreciate any feedback on the matter. I have been searching online for a > protocol of Picrosirius stain for frozens, but no luck. > > Thank you in advance, > > > > Yoanna Bello > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Laura Avogaro University of Trento Via delle Regole, 101 38123 Mattarello (TN) ? Italy Tel: +39 0461 283425 From ngauti <@t> lsuhsc.edu Tue Nov 12 08:32:24 2013 From: ngauti <@t> lsuhsc.edu (Gautier, Nicole M.) Date: Tue Nov 12 08:32:36 2013 Subject: [Histonet] coverslipping question Message-ID: My lab has been having a problem with "specks" appearing on our slides after they come out of Histoclear. Our protocol is to dehydrate in 2 minutes each of 70%, 80%, 95%, and 100% ethanol before 2 minutes 2 times in Histoclear. The slides are perfectly clear when they come out of the ethanols, but not when they come out of the Histoclear. Since it's not a problem we had when we first started, I tried changing out the Histoclear, but the problem remained. At the end of last week, I changed out everything - all glassware, all ethanols, all Histoclear. The problem went away and the 24 slides looked fine. Today, the slides are looking speckled again. So I guess my questions are: What could be causing the speckled appearance of the slides? and How often should I have to change the ethanols and Histoclear? I seem to remember years ago when I did this that we only changed the solutions monthly... Nicky Gautier Research Associate From mroark <@t> sfmc.net Tue Nov 12 09:02:36 2013 From: mroark <@t> sfmc.net (Matthew D. Roark) Date: Tue Nov 12 09:02:45 2013 Subject: [Histonet] Embedding Paraffin Message-ID: <257bc53f75814f46ac9b2311373a9e92@BLUPR04MB199.namprd04.prod.outlook.com> What paraffin does everyone like for embedding? We are currently using Surgipaths EM-400 but its dirty! Who has a clean, easy to section paraffin that they like? Thanks! Matthew Roark- HT/HTL(ASCP)CM Histology Specialist Saint Francis Medical Center 211 Saint Francis Drive Cape Girardeau, MO 63703 573-331-3982 mroark@sfmc.net http://www.sfmc.net From mmackinnon <@t> dermatologyconsultants.com Tue Nov 12 09:41:13 2013 From: mmackinnon <@t> dermatologyconsultants.com (Michelle M. Mackinnon) Date: Tue Nov 12 09:41:55 2013 Subject: [Histonet] RE: Embedding Paraffin In-Reply-To: <257bc53f75814f46ac9b2311373a9e92@BLUPR04MB199.namprd04.prod.outlook.com> References: <257bc53f75814f46ac9b2311373a9e92@BLUPR04MB199.namprd04.prod.outlook.com> Message-ID: <7141C086431ED24FB7F1E0E66028BC1B0C387836@mail.derm.local> We use Richard-Allan Paraffin Type 9. Embed nice and cuts beautifully for our lab. dermatology CONSULTANTS skin. care. experts. NOTICE OF CONFIDENTIALITY: The information in this email may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipeint, you are hereby notified that any dissemination, distrubution, or copying of this email is strictly prohibited. If you have received this email in error, please notify us immediately by replying to this email and deleting it from your computer. Thank you Dermatology Consultants, PA Please consider the environment before printing. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Matthew D. Roark Sent: Tuesday, November 12, 2013 9:03 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Embedding Paraffin What paraffin does everyone like for embedding? We are currently using Surgipaths EM-400 but its dirty! Who has a clean, easy to section paraffin that they like? Thanks! Matthew Roark- HT/HTL(ASCP)CM Histology Specialist Saint Francis Medical Center 211 Saint Francis Drive Cape Girardeau, MO 63703 573-331-3982 mroark@sfmc.net http://www.sfmc.net _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From trathborne <@t> somerset-healthcare.com Tue Nov 12 09:41:39 2013 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Tue Nov 12 09:42:16 2013 Subject: [Histonet] Leica vs Ventana In-Reply-To: References: Message-ID: <3AD061FE740D464FAC7BF6B5CFB75707A8B30752@smcmail02.somerset-healthcare.com> Another suggestion would be to go through the Histonet archives to see what problems people have questioned/reported over the last year. Don't forget about the bulk waste either. When we had considered Ventana, the waste (hazardous/non-hazardous) was combined - it may not be now, I haven't checked lately. Be sure to check with your own local municipality with regard to disposal. This could end up being far more costly for your lab if you have to pay for the combined bulk disposal rather than just the separated hazardous waste. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Phyllis Burnette Sent: Monday, November 11, 2013 5:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica vs Ventana Our hospital facility, as well, are faithful Ventana customers. We have 3 ultras and appreciate the ease and flexibility needed for a busy hospital facility. The slide drawers are random access so the slides are staining independently from one another. Another great feature of the ultra is the random access points where antibodies, exhausted reagents, etc. can be added once the run starts and at multiple times during the staining run. Our laboratory has found that the quality of the IHC stains are exceptional and consistent from lot to lot. The sales staff and technical support is superior and the engineers that work on our equipment are very thorough. Of course, Ventana is light-years above with specimen tracking for the histology laboratory and we are very excited that with this addition, all slides will be tracked from start to finish. Can't say enough, we really love this product!!! Phyllis S. Burnette HT, ASCP Anatomicals Supervisor Riverside Regional Medical Center 757-594-2885 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TGoins <@t> mt.gov Tue Nov 12 10:13:40 2013 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Tue Nov 12 10:14:05 2013 Subject: [Histonet] RE: Embedding Paraffin In-Reply-To: <257bc53f75814f46ac9b2311373a9e92@BLUPR04MB199.namprd04.prod.outlook.com> References: <257bc53f75814f46ac9b2311373a9e92@BLUPR04MB199.namprd04.prod.outlook.com> Message-ID: Paraplast X-tra by McCormick. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Matthew D. Roark Sent: Tuesday, November 12, 2013 8:03 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Embedding Paraffin What paraffin does everyone like for embedding? We are currently using Surgipaths EM-400 but its dirty! Who has a clean, easy to section paraffin that they like? Thanks! Matthew Roark- HT/HTL(ASCP)CM Histology Specialist Saint Francis Medical Center 211 Saint Francis Drive Cape Girardeau, MO 63703 573-331-3982 mroark@sfmc.net http://www.sfmc.net _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Valerie.Hannen <@t> parrishmed.com Tue Nov 12 10:23:43 2013 From: Valerie.Hannen <@t> parrishmed.com (Hannen, Valerie) Date: Tue Nov 12 10:23:48 2013 Subject: [Histonet] RE: Embedding Paraffin In-Reply-To: Message-ID: <450B7A81EDA0C54E97C53D60F00776C3232B4BB6A2@isexstore03> We are using Ameriplast LP which is distributed by Cardinal Health. We were using Paraplast X-tra, but due to cost savings we switched to the Ameriplast (which in my opinion is better than the Paraplast X-tra). -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Goins, Tresa Sent: Tuesday, November 12, 2013 11:14 AM To: Matthew D. Roark; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Embedding Paraffin Paraplast X-tra by McCormick. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Matthew D. Roark Sent: Tuesday, November 12, 2013 8:03 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Embedding Paraffin What paraffin does everyone like for embedding? We are currently using Surgipaths EM-400 but its dirty! Who has a clean, easy to section paraffin that they like? Thanks! Matthew Roark- HT/HTL(ASCP)CM Histology Specialist Saint Francis Medical Center 211 Saint Francis Drive Cape Girardeau, MO 63703 573-331-3982 mroark@sfmc.net http://www.sfmc.net _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet =================== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" =================== From mucram11 <@t> comcast.net Tue Nov 12 10:33:25 2013 From: mucram11 <@t> comcast.net (Pam Marcum) Date: Tue Nov 12 10:33:57 2013 Subject: [Histonet] Embedding Paraffin In-Reply-To: <257bc53f75814f46ac9b2311373a9e92@BLUPR04MB199.namprd04.prod.outlook.com> Message-ID: <31558753.1801864.1384274005607.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> We use Polyscientific R&D Paraffin Prills and love it.? The price has been excellent and it is a very clean product.? Too ma ny changes in other companies leading to base product issues?I just did not have the time or patience for, so?I went with the best?I could find.? I used it at the UPENN Lar ge Animal Orthopedic Laboratory and then switched?here when?we started to have issues with the previous vendor. ? Pam Marcum UAMS ----- Original Message ----- From: "Matthew D. Roark" To: histonet@lists.utsouthwestern.edu Sent: Tuesday, November 12, 2013 9:02:36 AM Subject: [Histonet] Embedding Paraffin What ?paraffin does everyone like for embedding? ?We are currently using Surgipaths EM-400 but its dirty! ? ?Who has a clean, easy to section paraffin that they like? Thanks! Matthew Roark- HT/HTL(ASCP)CM Histology Specialist Saint Francis Medical Center 211 Saint Francis Drive Cape Girardeau, MO 63703 573-331-3982 mroark@sfmc.net http://www.sfmc.net _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mw <@t> personifysearch.com Tue Nov 12 10:38:02 2013 From: mw <@t> personifysearch.com (Matt Ward) Date: Tue Nov 12 10:38:08 2013 Subject: [Histonet] New Field Based Histology Opportunities Message-ID: <111401cedfc5$8eff2400$acfd6c00$@personifysearch.com> Good morning! We have had 2 new field based IHC opportunities open. We are currently searching for experienced Histology/IHC professionals to work as Field Support Specialists with a leader in cancer diagnostics. The positions are based in the Northeast (MA or Upstate NY), and in San Diego, CA. Please e-mail me directly to learn more mw@personifysearch.com . Have a great day! Matt Ward Account Executive Personify 5020 Weston Parkway Suite 315 Cary NC 27513 (Tel) 800.875.6188 direct ext 103 (Fax) 919.460.0642 www.personifysearch.com From pscott <@t> scigene.com Tue Nov 12 12:59:25 2013 From: pscott <@t> scigene.com (Paul Scott) Date: Tue Nov 12 12:59:29 2013 Subject: [Histonet] Barcode Labels for slides Message-ID: <002401cedfd9$4e9c5a90$ebd50fb0$@com> Hi Histonetters, Can anyone recommend barcode labels to put on slides that will withstand FISH processing? The ones that we tried are coming off in the baths! Many thanks, paul Paul Scott SciGene 470 Lakeside Drive, Ste F, Sunnyvale, CA 94085-4720 408-733-7337 x 305 pscott@scigene.com Automating FISH and CMA Workflows www.SciGene.com From amosbrooks <@t> gmail.com Tue Nov 12 17:59:38 2013 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Tue Nov 12 17:59:46 2013 Subject: [Histonet] PicroSirius Red in Frozen Sections Message-ID: Peggy, That was a fantastic answer and it is responses like that to such questions that is the whole reason I enjoy the Histonet so much. Thank you for such a well thought out answer. Amos On Tue, Nov 12, 2013 at 1:00 PM, wrote: > Message: 7 > Date: Mon, 11 Nov 2013 19:34:32 -0500 > From: "Lee & Peggy Wenk" > Subject: Re: [Histonet] PicroSirius Red in Frozen Sections > To: "Yoanna Bello Arredondo" , > > Message-ID: <2ABC0BE3B30C4B8091C82B0CC2CA35F1@HP2010> > Content-Type: text/plain; format=flowed; charset="utf-8"; > reply-type=original > > I'm going to try to take a stab at this, but I don't know specifically > about > the picrosirius red stain. I'm going to talk in general about stains and > fixation (or lack thereof in the frozen section (FS)), and the relationship > between fixation and staining. I'm going to start out very simple, and then > get a little more complicated, so hang in there. > <<< SNIPPED >>> From amosbrooks <@t> gmail.com Tue Nov 12 18:15:43 2013 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Tue Nov 12 18:15:47 2013 Subject: [Histonet] Speaking of picrosirius red... Message-ID: Hi, In light of the current discussion about picrosirius red I would like to revisit a question that was asked a while back. If it was answered publicly, I apologize I probably missed it and didn't see it in the archives. Someone asked what is the purpose of the phosphomolybdic acid step before the picrosirius red. If anyone has a good answer for this I would like to know. While it is mostly curiosity, I think knowing what each step in a stain is for helps keep the quality and consistency of stains. Thanks, Amos From pruegg <@t> ihctech.net Tue Nov 12 19:31:39 2013 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Tue Nov 12 19:31:43 2013 Subject: [Histonet] Speaking of picrosirius red... In-Reply-To: References: Message-ID: I have done picrosirius red for years and never used phosphomolybdic acid so it would be hard to say what it's purpose is, mine works fine without it. > Date: Tue, 12 Nov 2013 19:15:43 -0500 > From: amosbrooks@gmail.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Speaking of picrosirius red... > > Hi, > In light of the current discussion about picrosirius red I would like > to revisit a question that was asked a while back. If it was answered > publicly, I apologize I probably missed it and didn't see it in the > archives. Someone asked what is the purpose of the phosphomolybdic acid > step before the picrosirius red. If anyone has a good answer for this I > would like to know. While it is mostly curiosity, I think knowing what each > step in a stain is for helps keep the quality and consistency of stains. > > Thanks, > Amos > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From cmiller <@t> gladstone.ucsf.edu Tue Nov 12 19:40:00 2013 From: cmiller <@t> gladstone.ucsf.edu (Caroline Miller) Date: Tue Nov 12 19:40:05 2013 Subject: [Histonet] Speaking of picrosirius red... In-Reply-To: References: Message-ID: Same here....... Caroline Miller Gladstone Institutes www.gladstoneinstitutes.org Tel: 415 7342566 Cell: 415 2187297 > On Nov 12, 2013, at 5:31 PM, Patsy Ruegg wrote: > > I have done picrosirius red for years and never used phosphomolybdic acid so it would be hard to say what it's purpose is, mine works fine without it. > >> Date: Tue, 12 Nov 2013 19:15:43 -0500 >> From: amosbrooks@gmail.com >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] Speaking of picrosirius red... >> >> Hi, >> In light of the current discussion about picrosirius red I would like >> to revisit a question that was asked a while back. If it was answered >> publicly, I apologize I probably missed it and didn't see it in the >> archives. Someone asked what is the purpose of the phosphomolybdic acid >> step before the picrosirius red. If anyone has a good answer for this I >> would like to know. While it is mostly curiosity, I think knowing what each >> step in a stain is for helps keep the quality and consistency of stains. >> >> Thanks, >> Amos >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rose.webb <@t> yale.edu Wed Nov 13 08:19:46 2013 From: rose.webb <@t> yale.edu (Webb, Rose) Date: Wed Nov 13 08:19:51 2013 Subject: [Histonet] Speaking of picrosirius red... In-Reply-To: Message-ID: Hi, I use a 0.2% phosphomolybdic acid step when I'm staining cardiac muscle. Just a quick (2 minutes) before the picrosirius red step. Without the phosphomolybdic acid sometimes the myocytes stain really yellow and that makes it more difficult to see the type 1 collagen. If you don't do the phophomolybdic acid step usually extending the dehydration steps will get rid of the background as well. If what you are interested in is the birefringence of the collagen, the yellow background doesn't matter. And, some people like that yellow staining, I only consider it a problem on the cardiac muscle. -Rose On 11/12/13 7:15 PM, "Amos Brooks" wrote: >Hi, > In light of the current discussion about picrosirius red I would like >to revisit a question that was asked a while back. If it was answered >publicly, I apologize I probably missed it and didn't see it in the >archives. Someone asked what is the purpose of the phosphomolybdic acid >step before the picrosirius red. If anyone has a good answer for this I >would like to know. While it is mostly curiosity, I think knowing what >each >step in a stain is for helps keep the quality and consistency of stains. > >Thanks, >Amos >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Ken_Marissael <@t> vwr.com Wed Nov 13 13:04:02 2013 From: Ken_Marissael <@t> vwr.com (Ken_Marissael@vwr.com) Date: Wed Nov 13 13:04:12 2013 Subject: [Histonet] Ken Marissael no longer with VWR. Message-ID: I will be out of the office starting 11/12/2013 and will not return until 11/30/2013. Please contact - healthcareservice.com or customer service at 877-881-1192. From one_angel_secret <@t> yahoo.com Wed Nov 13 13:35:58 2013 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Wed Nov 13 13:36:12 2013 Subject: [Histonet] coverslipping question In-Reply-To: References: Message-ID: I would try adding 1 or 2 more 100% alcohols before the histoclear. Sent from my iPhone On Nov 12, 2013, at 9:32 AM, "Gautier, Nicole M." wrote: > My lab has been having a problem with "specks" appearing on our slides after they come out of Histoclear. Our protocol is to dehydrate in 2 minutes each of 70%, 80%, 95%, and 100% ethanol before 2 minutes 2 times in Histoclear. The slides are perfectly clear when they come out of the ethanols, but not when they come out of the Histoclear. > Since it's not a problem we had when we first started, I tried changing out the Histoclear, but the problem remained. At the end of last week, I changed out everything - all glassware, all ethanols, all Histoclear. The problem went away and the 24 slides looked fine. Today, the slides are looking speckled again. > So I guess my questions are: What could be causing the speckled appearance of the slides? and How often should I have to change the ethanols and Histoclear? I seem to remember years ago when I did this that we only changed the solutions monthly... > > Nicky Gautier > Research Associate > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From one_angel_secret <@t> yahoo.com Wed Nov 13 13:37:37 2013 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Wed Nov 13 13:37:50 2013 Subject: [Histonet] Embedding Paraffin In-Reply-To: <257bc53f75814f46ac9b2311373a9e92@BLUPR04MB199.namprd04.prod.outlook.com> References: <257bc53f75814f46ac9b2311373a9e92@BLUPR04MB199.namprd04.prod.outlook.com> Message-ID: <6B88BB96-686E-4CB9-8F8D-DFE936BE98A4@yahoo.com> We use formulae R from Leica for both infiltrating and embedding with excellent results. Sent from my iPhone On Nov 12, 2013, at 10:02 AM, "Matthew D. Roark" wrote: > What paraffin does everyone like for embedding? We are currently using Surgipaths EM-400 but its dirty! Who has a clean, easy to section paraffin that they like? > > Thanks! > > > Matthew Roark- HT/HTL(ASCP)CM > Histology Specialist > Saint Francis Medical Center > 211 Saint Francis Drive > Cape Girardeau, MO 63703 > 573-331-3982 > mroark@sfmc.net > http://www.sfmc.net > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From one_angel_secret <@t> yahoo.com Wed Nov 13 13:58:23 2013 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Wed Nov 13 13:58:38 2013 Subject: [Histonet] Frozen IF Specimen Storage: Is -80 C really necessary; will -20 C work? In-Reply-To: <6D6BD1DE8A5571489398B392A38A71579D470AAE@xmdb04.nch.kids> References: <1384186142.72388.YahooMailNeo@web163101.mail.bf1.yahoo.com> <6D6BD1DE8A5571489398B392A38A71579D470AAE@xmdb04.nch.kids> Message-ID: <878ABEBD-FAAA-42FE-984F-F2768A5AFBC4@yahoo.com> I was worried about this when I first started doing DIF's here. All I have is a -20 -25c freezer. At the direction if our pathologist I was challenged to do it and watch it closely which is what I have been doing for the past year. When I am done cutting the frozen sections I place Oct back on the sample to enclose it. Then I put it in a sealed bag then place it into a styrofoam sealed container I have in the freezer. We have excellent results and the controls have been fabulous so far one year in. I could probably load a picture of it if any were interested. Anyway this is what we are doing and getting very good results working with what we have and always being diligent of paying attention to the quality of our controls. If I see a decline in quality I'll be the first to scream for a new fridge like I was in the beginning. Until then I'd have to say it's working very well. And a one year keep for my controls I can live with. Sent from my iPhone On Nov 11, 2013, at 5:43 PM, "Tony Henwood (SCHN)" wrote: > The following (from "The Book" - that may never be published!): > > It is also important to remember that -20oC is not suitable for storing frozen tissue. Have you ever noticed that meat stored too long in the freezer suffers "freezer-burn"; it appears dried-out? The condition is primarily caused by sublimation. Water evaporates at all temperatures, even from the surface of solid ice. If air adjacent to ice is cold enough (so the ice won't melt) and the air is dry enough, water molecules go directly from solid phase (ice) to gaseous phase (vapour) without going through a liquid phase. When the constantly vibrating water molecules in foods stored in a freezer migrate to the surface, crystals of ice outside of the solid food are formed, and some water molecules escape into the air (by sublimation). The meat parts now deprived of moisture become dry and shrivelled and look burnt. In meats, air can cause fats to oxidize. > > Frost-free freezers are the result of having a fan and air circulation inside the freezer. The sub-zero temperature combined with the air circulation that keeps the air arid significantly accelerates the sublimation process. This keeps freezer walls and shelves free of ice, although ice-cubes will continually sublimate. > > Regards > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) > Laboratory Manager & Senior Scientist, the Children's Hospital at Westmead > Adjunct Fellow, School of Medicine, University of Western Sydney > > Tel: 612 9845 3306 > Fax: 612 9845 3318 > Pathology Department > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead > Locked Bag 4001, Westmead NSW 2145, AUSTRALIA > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa > Sent: Tuesday, 12 November 2013 3:09 AM > To: DAVID HENDERSON; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Frozen IF Specimen Storage: Is -80 C really necessary; will -20 C work? > > I really do not know of any study comparing -20?C vs -80?C or if this -80?C is the result of a commercially driven "ideal" storage method. I advise you to check about any bibliographic references BUT meanwhile I also advise you to keep your precious and irreplaceable material at -80?C. I do not think that you should risk losing all your samples just to work "on the cheap". > I always kept my tumor bank and IF samples at -80?C and when our freezer also died year ago, I was lucky enough to get the approval to buy a new one. > Do not rush to save money > Ren? J. > > > ________________________________ > From: DAVID HENDERSON > To: "histonet@lists.utsouthwestern.edu" > Sent: Sunday, November 10, 2013 6:56 PM > Subject: [Histonet] Frozen IF Specimen Storage: Is -80 C really necessary; will -20 C work? > > > We routinely store our frozen IF specimens (renal and skin biopsies) embedded in OCT and sealed in plastic bags for at least 4 yrs in a -80C freezer. Cases that are positively diagnosed by the pathologist, are used as positive controls for subsequent IF staining. In practice, most positive controls are used within 1-2 years of their original storage. > > Our -80C freezer has died, and we are temporarily storing in a different -80C freezer in another building on campus. My supervisor asked me to research whether or not a -20C freezer will suffice for our storage needs, or must we anti up for the much more costly -80C replacement or repair. > > I would appreciate receiving suggestions backed by experience, or educated thoughts about the advisability of -20C storage of specimens for future IF staining. > > Thanks, > David Henderson, HTL > RML, Tulsa, OK > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ********************************************************************************* > This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. > > Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. > > This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. > ********************************************************************************* > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From estellamireles <@t> gmail.com Wed Nov 13 14:18:14 2013 From: estellamireles <@t> gmail.com (Stella Mireles) Date: Wed Nov 13 14:18:18 2013 Subject: [Histonet] Rolling of my ribbon ? Another paraffin question. Message-ID: *I am presently using a paraffin designated as an IM product.* *We are a facility that cuts only autopsies and have been experiencing alot of rolling of our sections.* *We did recently switch to this product, because of cost.* *Question : Is the paraffin you are using working well on autopsy tissue and producing ribbons right away ? Do you use it for infiltration as well as embedding ?* *Thank you for your assistant.* *Stella Walters* From philip_manfre <@t> merck.com Wed Nov 13 14:30:41 2013 From: philip_manfre <@t> merck.com (Manfre, Philip) Date: Wed Nov 13 14:30:50 2013 Subject: [Histonet] Rolling of my ribbon ? Another paraffin question. In-Reply-To: References: Message-ID: <558A4571351D0C42BD923F403F4198C4B48D475E8C@USCTMXP51014.merck.com> Try running a wooden dowel (stick) lightly across your blade, dulling it slightly. Disposable blades can actually be too sharp sometimes. Philip Manfre, B.A., HT (ASCP) Associate Principal Scientist Merck Research Laboratories WP45-251 PO Box 4 West Point, PA 19486 215-652-9750 215-993-0383 (fax) philip_manfre@merck.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stella Mireles Sent: Wednesday, November 13, 2013 3:18 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Rolling of my ribbon ? Another paraffin question. *I am presently using a paraffin designated as an IM product.* *We are a facility that cuts only autopsies and have been experiencing alot of rolling of our sections.* *We did recently switch to this product, because of cost.* *Question : Is the paraffin you are using working well on autopsy tissue and producing ribbons right away ? Do you use it for infiltration as well as embedding ?* *Thank you for your assistant.* *Stella Walters* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From Joyce.Weems <@t> emoryhealthcare.org Wed Nov 13 14:36:36 2013 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Wed Nov 13 14:36:42 2013 Subject: [Histonet] Rolling of my ribbon ? Another paraffin question. In-Reply-To: <558A4571351D0C42BD923F403F4198C4B48D475E8C@USCTMXP51014.merck.com> References: <558A4571351D0C42BD923F403F4198C4B48D475E8C@USCTMXP51014.merck.com> Message-ID: A cork works well for this purpose too.. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Manfre, Philip Sent: Wednesday, November 13, 2013 3:31 PM To: Stella Mireles; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Rolling of my ribbon ? Another paraffin question. Try running a wooden dowel (stick) lightly across your blade, dulling it slightly. Disposable blades can actually be too sharp sometimes. Philip Manfre, B.A., HT (ASCP) Associate Principal Scientist Merck Research Laboratories WP45-251 PO Box 4 West Point, PA 19486 215-652-9750 215-993-0383 (fax) philip_manfre@merck.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stella Mireles Sent: Wednesday, November 13, 2013 3:18 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Rolling of my ribbon ? Another paraffin question. *I am presently using a paraffin designated as an IM product.* *We are a facility that cuts only autopsies and have been experiencing alot of rolling of our sections.* *We did recently switch to this product, because of cost.* *Question : Is the paraffin you are using working well on autopsy tissue and producing ribbons right away ? Do you use it for infiltration as well as embedding ?* *Thank you for your assistant.* *Stella Walters* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From flnails <@t> texaschildrens.org Wed Nov 13 16:46:04 2013 From: flnails <@t> texaschildrens.org (Nails, Felton) Date: Wed Nov 13 16:46:10 2013 Subject: [Histonet] neuropath stains Message-ID: <327E034F1892504289B7A17EC71DF9F3052393@TCFMSG03.ad.texaschildrenshospital.org> Are the basic muscle and nerve biopsy stains being performed manually or has someone automated these stains? ______________________________________________________________________ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ______________________________________________________________________ From Timothy.Morken <@t> ucsfmedctr.org Wed Nov 13 16:58:13 2013 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Wed Nov 13 16:58:25 2013 Subject: [Histonet] RE: neuropath stains In-Reply-To: <327E034F1892504289B7A17EC71DF9F3052393@TCFMSG03.ad.texaschildrenshospital.org> References: <327E034F1892504289B7A17EC71DF9F3052393@TCFMSG03.ad.texaschildrenshospital.org> Message-ID: <761E2B5697F795489C8710BCC72141FF0B1D4D@ex07.net.ucsf.edu> Felton, we are still doing them manually. Some of them could be done on the newer batch stainers that have heated reagent stations, but the incubation periods are pretty long so it may not be practical. At least you would probably need a dedicated instrument. Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nails, Felton Sent: Wednesday, November 13, 2013 2:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] neuropath stains Are the basic muscle and nerve biopsy stains being performed manually or has someone automated these stains? ______________________________________________________________________ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Wed Nov 13 17:04:46 2013 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Wed Nov 13 17:04:56 2013 Subject: [Histonet] RE: neuropath stains In-Reply-To: <761E2B5697F795489C8710BCC72141FF0B1D4D@ex07.net.ucsf.edu> References: <327E034F1892504289B7A17EC71DF9F3052393@TCFMSG03.ad.texaschildrenshospital.org> <761E2B5697F795489C8710BCC72141FF0B1D4D@ex07.net.ucsf.edu> Message-ID: <761E2B5697F795489C8710BCC72141FF0B1D78@ex07.net.ucsf.edu> ...Although the cost of batch staining would be high for some of these reagents. I have asked immunostainer vendors about this and none I have talked to have done anything along those lines Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Wednesday, November 13, 2013 2:58 PM To: 'Nails, Felton'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: neuropath stains Felton, we are still doing them manually. Some of them could be done on the newer batch stainers that have heated reagent stations, but the incubation periods are pretty long so it may not be practical. At least you would probably need a dedicated instrument. Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nails, Felton Sent: Wednesday, November 13, 2013 2:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] neuropath stains Are the basic muscle and nerve biopsy stains being performed manually or has someone automated these stains? ______________________________________________________________________ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Bruce_Palmatier <@t> vwr.com Wed Nov 13 17:56:51 2013 From: Bruce_Palmatier <@t> vwr.com (Bruce_Palmatier@vwr.com) Date: Wed Nov 13 17:57:05 2013 Subject: [Histonet] AUTO: Bruce Palmatier is out of the office (returning 11/14/2013) Message-ID: I am out of the office until 11/14/2013. I will be out of the office on Nov 13. If you have a question about an order or need assistance placing an order, or if the matter is urgent, please call 877-881-1192 between 8am and 8pm and a VWR Healthcare Service associate will assist you. For other matters, I will respond to emails within 24 hours. If you require pricing information, quotes, product information, or other assistance, Tim Rafferty is the new VWR Healthcare Sales Rep who's taken my place. He can be reached at timothy_rafferty@vwr.com or (717) 668-9045. Thank You, Bruce Palmatier Market Portfolio Manager VWR Healthcare bruce_palmatier@vwr.com mobile: 484.319.5563 fax: 484-881-7307 Customer Service: 877.881.1192 Fax: 484.881.6486 Customer Service email: HEALTHCARESERVICE@VWR.COM Note: This is an automated response to your message "Histonet Digest, Vol 120, Issue 16" sent on 11/13/2013 1:01:39 PM. This is the only notification you will receive while this person is away. From gu.lang <@t> gmx.at Thu Nov 14 01:32:40 2013 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Thu Nov 14 01:32:43 2013 Subject: AW: [Histonet] Rolling of my ribbon ? Another paraffin question. In-Reply-To: References: Message-ID: <000901cee10b$b39688c0$1ac39a40$@gmx.at> Has this new paraffin a higher melting point than the former? It may happen that the block/paraffin is too hard at your cutting temperature to stick. Does it make ribbons after a while trying? Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Stella Mireles Gesendet: Mittwoch, 13. November 2013 21:18 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Rolling of my ribbon ? Another paraffin question. *I am presently using a paraffin designated as an IM product.* *We are a facility that cuts only autopsies and have been experiencing alot of rolling of our sections.* *We did recently switch to this product, because of cost.* *Question : Is the paraffin you are using working well on autopsy tissue and producing ribbons right away ? Do you use it for infiltration as well as embedding ?* *Thank you for your assistant.* *Stella Walters* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpwenk <@t> sbcglobal.net Thu Nov 14 07:10:07 2013 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Thu Nov 14 07:10:12 2013 Subject: [Histonet] Rolling of my ribbon ? Another paraffin question. In-Reply-To: References: Message-ID: <8C653F8BE2F844359FA2A5950D7210AC@HP2010> Just a thought - have you tried changing the angle of the blade? Each different type of blade from different vendors need a different angle for microtoming. Each different type of microtome from different vendors need a different angle for microtoming. I know these two facts, as I have tried different vendors' blades, and have had to change the angle on the microtome to get a good ribbon. The School also has different vendors' microtomes in the School, all using the same blades, and they had to be set at different angles. Plus, I have talked with vendors of various microtomes and various blades, and those who really know microtomy, especially those were were/are histotechs, will tell you that the blade angles need to be changed depending upon which blade is being used at which microtome. And also, from year to year, with different students sitting at the "same" microtome, we've also had to change the angles, depending on how the student cuts. We haven't changed brands of paraffin in the long time, but I'm wondering if we would need to change the angle of blade if we got a different paraffin. The easiest way to check is to move the knife angle all the way to one end (for example, on the vendor's microtome that we have the most of in the School, that would be to # 10.) Try microtoming. If that doesn't help, move it one degree (what would be #9 if there were a number). Try microtoming again. Keep doing this (changing angles and microtoming) until you get the best ribbon. (We found it easier to start at one end and move "down", rather to start in the middle (say, #5), and then not know if we need to go up (#6) or go down (#4)). Let us know if knife angle is a factor with this new paraffin. I'd really like to know. Vendors - anyone know/have information that blade angle makes a difference with type of paraffin, for getting good ribbons? Peggy A. Wenk, HTL(ASCP)SLS -----Original Message----- From: Stella Mireles Sent: Wednesday, November 13, 2013 3:18 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Rolling of my ribbon ? Another paraffin question. *I am presently using a paraffin designated as an IM product.* *We are a facility that cuts only autopsies and have been experiencing alot of rolling of our sections.* *We did recently switch to this product, because of cost.* *Question : Is the paraffin you are using working well on autopsy tissue and producing ribbons right away ? Do you use it for infiltration as well as embedding ?* *Thank you for your assistant.* *Stella Walters* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b427297 <@t> aol.com Thu Nov 14 09:07:26 2013 From: b427297 <@t> aol.com (b427297@aol.com) Date: Thu Nov 14 09:07:31 2013 Subject: [Histonet] coverslipping question In-Reply-To: References: Message-ID: <8D0AF625CE57DCA-134C-1BC3@webmail-d218.sysops.aol.com> Are you using tape or glass coverslips? We have found that when using tape coverslips, at least two very clean 100% alcohol steps must be added before slides go into the clearing reagent, be it xylene or Histoclear. If not, brown spots appear on some tissues, usually liver and bone. If there is any TRACE of water retained in the tissue, it will show up as a brown artifact on the tissue. This doesn't happen with conventional glass coverslipping and mounting medium - I think the mounting medium compensates for the retained moisture in the tissues. Jackie -----Original Message----- From: Kim Donadio To: Gautier, Nicole M. Cc: histonet Sent: Wed, Nov 13, 2013 1:37 pm Subject: Re: [Histonet] coverslipping question I would try adding 1 or 2 more 100% alcohols before the histoclear. Sent from my iPhone On Nov 12, 2013, at 9:32 AM, "Gautier, Nicole M." wrote: > My lab has been having a problem with "specks" appearing on our slides after they come out of Histoclear. Our protocol is to dehydrate in 2 minutes each of 70%, 80%, 95%, and 100% ethanol before 2 minutes 2 times in Histoclear. The slides are perfectly clear when they come out of the ethanols, but not when they come out of the Histoclear. > Since it's not a problem we had when we first started, I tried changing out the Histoclear, but the problem remained. At the end of last week, I changed out everything - all glassware, all ethanols, all Histoclear. The problem went away and the 24 slides looked fine. Today, the slides are looking speckled again. > So I guess my questions are: What could be causing the speckled appearance of the slides? and How often should I have to change the ethanols and Histoclear? I seem to remember years ago when I did this that we only changed the solutions monthly... > > Nicky Gautier > Research Associate > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mucram11 <@t> comcast.net Thu Nov 14 09:36:21 2013 From: mucram11 <@t> comcast.net (Pam Marcum) Date: Thu Nov 14 09:36:38 2013 Subject: [Histonet] neuropath stains In-Reply-To: <327E034F1892504289B7A17EC71DF9F3052393@TCFMSG03.ad.texaschildrenshospital.org> Message-ID: <1160818614.1834157.1384443381975.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> So far when I ask about neuro stains being automated by any company the answer is no.? The volume clinically is not high enough and the times required would tie the stainers up from hours to overnight in for some steps.? It would almost require a dedicated stainer for neuro only and would be very expensive.? We do ours manually. ? Pam Marcum UAMS ----- Original Message ----- From: "Felton Nails" To: histonet@lists.utsouthwestern.edu Sent: Wednesday, November 13, 2013 4:46:04 PM Subject: [Histonet] neuropath stains Are the basic muscle and nerve biopsy stains being performed manually or has someone automated these stains? ______________________________________________________________________ CONFIDENTIALITY NOTICE: ?The information in this e-mail may be confidential and/or ?privileged. ?If you are not the intended recipient or an ?authorized representative of the intended recipient, you ?are hereby notified that any review, dissemination, or ?copying of this e-mail and its attachments, if any, or ?the information contained herein is prohibited. ?If you ?have received this e-mail in error, please immediately ?notify the sender by return e-mail and delete this e-mail ?from your computer system. ?Thank you. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LMurphy2 <@t> aultman.com Thu Nov 14 10:04:05 2013 From: LMurphy2 <@t> aultman.com (Leann M. Murphy) Date: Thu Nov 14 10:04:13 2013 Subject: [Histonet] (no subject) Message-ID: I was just wondering if anyone was having difficulty cutting biopsies? We have been using the same blades for years and now it is so difficult to get a ribbon. There are three other area hospitals having the same problem. I have been trying samples from various vendors and the problem is still the same. Maybe the company that makes these microtome blades have cut costs and is now using a lower grade of metal for the blade. I don't know what it is, but it is driving everyone crazy. Also, we are spending more money on blades because they do not last as long and of course this does not make our Manager happy. I am just very frustrated. Any suggestions? LeAnn Murph, HT (ASCP) Aultman Hosptial Canton, Ohio Technical Specialist From trathborne <@t> somerset-healthcare.com Thu Nov 14 10:19:51 2013 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Thu Nov 14 10:20:43 2013 Subject: [Histonet] (no subject) In-Reply-To: References: Message-ID: <3AD061FE740D464FAC7BF6B5CFB75707A8B31FE4@smcmail02.somerset-healthcare.com> I wouldn't be too quick to blame the blades. Have you changed paraffin brands/temperatures or changed any of your processing schedules? Is the fixative/fixation process the same? Is the collecting location doing anything different/have they had a change in staff? Also, what type of blades are you using? It would be easier for people to respond if they had this information. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Leann M. Murphy Sent: Thursday, November 14, 2013 11:04 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) I was just wondering if anyone was having difficulty cutting biopsies? We have been using the same blades for years and now it is so difficult to get a ribbon. There are three other area hospitals having the same problem. I have been trying samples from various vendors and the problem is still the same. Maybe the company that makes these microtome blades have cut costs and is now using a lower grade of metal for the blade. I don't know what it is, but it is driving everyone crazy. Also, we are spending more money on blades because they do not last as long and of course this does not make our Manager happy. I am just very frustrated. Any suggestions? LeAnn Murph, HT (ASCP) Aultman Hosptial Canton, Ohio Technical Specialist _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From claycal44 <@t> yahoo.com Thu Nov 14 10:30:34 2013 From: claycal44 <@t> yahoo.com (nancy lowen) Date: Thu Nov 14 10:30:39 2013 Subject: [Histonet] RE: Embedding Paraffin In-Reply-To: References: <257bc53f75814f46ac9b2311373a9e92@BLUPR04MB199.namprd04.prod.outlook.com> Message-ID: <1384446634.83005.YahooMailNeo@web125602.mail.ne1.yahoo.com> I use McCormick Paraplast Plus. On Tuesday, November 12, 2013 8:14 AM, "Goins, Tresa" wrote: Paraplast X-tra by McCormick. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Matthew D. Roark Sent: Tuesday, November 12, 2013 8:03 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Embedding Paraffin What? paraffin does everyone like for embedding?? We are currently using Surgipaths EM-400 but its dirty!? ? Who has a clean, easy to section paraffin that they like? Thanks! Matthew Roark- HT/HTL(ASCP)CM Histology Specialist Saint Francis Medical Center 211 Saint Francis Drive Cape Girardeau, MO 63703 573-331-3982 mroark@sfmc.net http://www.sfmc.net/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From algranth <@t> email.arizona.edu Thu Nov 14 10:45:48 2013 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Thu Nov 14 10:48:41 2013 Subject: [Histonet] RE: Embedding Paraffin In-Reply-To: <1384446634.83005.YahooMailNeo@web125602.mail.ne1.yahoo.com> References: <257bc53f75814f46ac9b2311373a9e92@BLUPR04MB199.namprd04.prod.outlook.com> <1384446634.83005.YahooMailNeo@web125602.mail.ne1.yahoo.com> Message-ID: We stumbled on Polyscientific's GemCut Pink Sapphire Parrafin and really like it. Before that we used Paraplast and it was OK but our blocks cut so much nicer with the GemCut. Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 From Timothy.Morken <@t> ucsfmedctr.org Thu Nov 14 10:55:02 2013 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Thu Nov 14 10:55:16 2013 Subject: [Histonet] Anyone using Section Transfer System by Microm? Message-ID: <761E2B5697F795489C8710BCC72141FF0B1F82@ex07.net.ucsf.edu> Is anyone using the Thermo/Microm Secton Transfer System on their microtome? http://cellab.se/pdfbroschyr/Microm/STS.pdf I'd like to get some input for using it to cut serial sections of microarrays. Thanks! Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center Box 1656 505 Parnassus Ave San Francisco, CA 94143 USA 415.353.1266 (office) tim.morken@ucsfmedctr.org From Sarah.Lewis <@t> nationwidechildrens.org Thu Nov 14 11:04:16 2013 From: Sarah.Lewis <@t> nationwidechildrens.org (Lewis, Sarah) Date: Thu Nov 14 11:04:20 2013 Subject: [Histonet] neuropath stains In-Reply-To: <327E034F1892504289B7A17EC71DF9F3052393@TCFMSG03.ad.texaschildrenshospital.org> References: <327E034F1892504289B7A17EC71DF9F3052393@TCFMSG03.ad.texaschildrenshospital.org> Message-ID: <4BAB3E4AF573804EA9929EFD1E158171243C779A@RESEXCHMBX01.CRII.ORG> Our immunofluorescence protein antibody stains are automated, but not the routine histochemical stains used for muscle biopsies. Between the importance of maintaining pH levels, incubation temperatures (SDH,NADH,COX, AtPase's, Amyloid, MAD, Myophos, Acid Phos) and the need of a rotator plate (ORO). Really the only options for automation would be the H&E, Gomori Trichrome and PAS. Sarah E. Lewis HT, ASCP Laboratory Coordinator The Research Institute at Nationwide Children's Hospital Center for Gene Therapy Neuromuscular Division Rm WA3110 (614)722-2204 -----Original Message----- From: Nails, Felton [mailto:flnails@texaschildrens.org] Sent: Wednesday, November 13, 2013 5:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] neuropath stains Are the basic muscle and nerve biopsy stains being performed manually or has someone automated these stains? ______________________________________________________________________ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ______________________________________________________________________ From JEllin <@t> yumaregional.org Thu Nov 14 11:13:02 2013 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Thu Nov 14 11:13:08 2013 Subject: [Histonet] RE: Anyone using Section Transfer System by Microm? In-Reply-To: <761E2B5697F795489C8710BCC72141FF0B1F82@ex07.net.ucsf.edu> References: <761E2B5697F795489C8710BCC72141FF0B1F82@ex07.net.ucsf.edu> Message-ID: I would also like to know -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Thursday, November 14, 2013 9:55 AM To: Histonet Subject: [Histonet] Anyone using Section Transfer System by Microm? Is anyone using the Thermo/Microm Secton Transfer System on their microtome? http://cellab.se/pdfbroschyr/Microm/STS.pdf I'd like to get some input for using it to cut serial sections of microarrays. Thanks! Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center Box 1656 505 Parnassus Ave San Francisco, CA 94143 USA 415.353.1266 (office) tim.morken@ucsfmedctr.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ From patrick.lewis <@t> seattlechildrens.org Thu Nov 14 12:19:05 2013 From: patrick.lewis <@t> seattlechildrens.org (Lewis, Patrick) Date: Thu Nov 14 12:19:34 2013 Subject: [Histonet] Has anyone had any problems with the Vector Immuedge pap pen? Message-ID: <3903BE18914F4440834F0E620415FFCA381F6F28@PPWEXD01a.childrens.sea.kids> Hi everyone, Has anyone had any problems with the Immedge Pap pen from Vector. Cat # H-4000 It gives a nice wide barrier, but for some reason the barrier is disintegrating on our slides so I get holes in the lines I draw. I have had a similar problem before, usually because the slide wasn't dry enough when I was drawing the barrier or I was immersing the slide before the barrier had a chance to solidify. However, the problem has never been this bad. I am wondering if my current batch of pap pens is defective. Patrick CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From Maxim_71 <@t> mail.ru Thu Nov 14 12:51:42 2013 From: Maxim_71 <@t> mail.ru (Maxim Peshkov) Date: Thu Nov 14 12:51:49 2013 Subject: [Histonet] Anyone using Section Transfer System by Microm? Message-ID: <531719249.20131114225142@mail.ru> Tim, HM340 + STS now successfully uses in many Russians hisology labs and techs loves them. STS have the one disadvantage: is need to much time for cleaning and maintenance vs waterbath. Also it is necessary some skills for using it. Sincerely, Maxim Peshkov, Russia, Taganrog. mailto:Maxim_71@mail.ru From Klaus.Kruttwig <@t> ucsf.edu Thu Nov 14 14:35:43 2013 From: Klaus.Kruttwig <@t> ucsf.edu (Kruttwig, Klaus) Date: Thu Nov 14 14:36:41 2013 Subject: [Histonet] Has anyone had any problems with the Vector Immuedge pap pen? In-Reply-To: <3903BE18914F4440834F0E620415FFCA381F6F28@PPWEXD01a.childrens.sea.kids> References: <3903BE18914F4440834F0E620415FFCA381F6F28@PPWEXD01a.childrens.sea.kids> Message-ID: Hi Patrick, yes, I had a similar problem with the Immedge Pap pen. I took very long before I could immerse the sections again with buffer and the barrier still collapsed later on. I used a quite old one that I found in the lab so I guess they stop functioning after a while. Klaus Klaus Kruttwig Department of Cell and Tissue Biology University of California, San Francisco HSW601 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Lewis, Patrick [patrick.lewis@seattlechildrens.org] Sent: Thursday, November 14, 2013 10:19 AM To: 'Histonet@lists.utsouthwestern.edu' Subject: [Histonet] Has anyone had any problems with the Vector Immuedge pap pen? Hi everyone, Has anyone had any problems with the Immedge Pap pen from Vector. Cat # H-4000 It gives a nice wide barrier, but for some reason the barrier is disintegrating on our slides so I get holes in the lines I draw. I have had a similar problem before, usually because the slide wasn't dry enough when I was drawing the barrier or I was immersing the slide before the barrier had a chance to solidify. However, the problem has never been this bad. I am wondering if my current batch of pap pens is defective. Patrick CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis <@t> bresnan.net Thu Nov 14 16:03:00 2013 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Thu Nov 14 16:03:08 2013 Subject: [Histonet] Re: ImmEdge Pap Pen problems Message-ID: <000001cee185$4a956dd0$dfc04970$@bresnan.net> The problem is using the pen without redistributing the liquid and solid contents in the pen. Years ago, the Vector technical service rep said to shake the daylights out of the pen. We use a vortex mixer, at highest speed and press hard, to make sure the contents were well mixed before use. We then have no problems of the "goo" lifting off the slide when in buffer or using a solvent fixative for frozen sections after applying the barrier. We had pens that worked for years as long as we shook (or vortexed) them well before applying the barrier around sections. We used the pens for both deparaffinized slides (well dried around section) and air dried frozen section (on dry slides). Good luck Gayle Callis HTL/HT/MT(ASCP) From Jonathan.Cremer <@t> med.kuleuven.be Fri Nov 15 01:17:06 2013 From: Jonathan.Cremer <@t> med.kuleuven.be (Jonathan Cremer) Date: Fri Nov 15 01:17:28 2013 Subject: [Histonet] RE: Anyone using Section Transfer System by Microm? In-Reply-To: <761E2B5697F795489C8710BCC72141FF0B1F82@ex07.net.ucsf.edu> References: <761E2B5697F795489C8710BCC72141FF0B1F82@ex07.net.ucsf.edu> Message-ID: Yes, and I wouldn't be able to cut without it anymore. It is quite some work to keep it clean (and you have to keep it clean, or you'll get algae growing in places you didn't even know about). Cutting a ribbon and picking it up on a slide is a breeze, cutting serials goes really fast if you let the sections stretch on the slides instead of on the waterbath. --- Jonathan Cremer Laboratory Technician TARGID - KU Leuven Gasthuisberg CDG; Labo Experimentele Immunologie; Herestraat 49 bus 811; 3000 Leuven; Belgium ________________________________________ Van: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] namens Morken, Timothy [Timothy.Morken@ucsfmedctr.org] Verzonden: donderdag 14 november 2013 17:55 Aan: Histonet Onderwerp: [Histonet] Anyone using Section Transfer System by Microm? Is anyone using the Thermo/Microm Secton Transfer System on their microtome? http://cellab.se/pdfbroschyr/Microm/STS.pdf I'd like to get some input for using it to cut serial sections of microarrays. Thanks! Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center Box 1656 505 Parnassus Ave San Francisco, CA 94143 USA 415.353.1266 (office) tim.morken@ucsfmedctr.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From FMonson <@t> wcupa.edu Fri Nov 15 09:07:16 2013 From: FMonson <@t> wcupa.edu (Monson, Frederick) Date: Fri Nov 15 09:07:25 2013 Subject: [Histonet] Rolling of my ribbon ? Another paraffin question. In-Reply-To: References: Message-ID: <899EAA40AE6E47488CE0637489EFB30C1804A29A@WCU-XCH-03.PASSHE.LCL> Dear Stella, Prior to buildings with locked windows, histologists who embedded with paraffin would fabricate, from just paraffin waxes of different hardness to match the climate of their geographic locations. Thus, http://www.microscopy-uk.org.uk/mag/artjan07/yl-para1w.html you can fabricate your own embedment with/or without the PEG (or whatever hygroscopic agent is added to provide 'stick') to match your needs. Most large labs will not want to do this, so perhaps adding a dehumidifier to the microtome room to adjust the humidity to the embedment. An accompanying message will describe one of my favorite mountants that requires an extended prep. Cheers, Fred Monson Frederick C. Monson, PhD Technical Director Center for Microanalysis and Imaging, Research and Training (CMIRT) West Chester University of Pennsylvania Schmucker Science South - Room SSS-024 MailDrop: Geology-Astronomy 750 South Church Street West Chester, PA, 19383 610-738-0437 fmonson@wcupa.edu HomePage: http://cmirt.wcupa.edu (with link to instrument Scheduler) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stella Mireles Sent: Wednesday, November 13, 2013 3:18 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Rolling of my ribbon ? Another paraffin question. *I am presently using a paraffin designated as an IM product.* *We are a facility that cuts only autopsies and have been experiencing alot of rolling of our sections.* *We did recently switch to this product, because of cost.* *Question : Is the paraffin you are using working well on autopsy tissue and producing ribbons right away ? Do you use it for infiltration as well as embedding ?* *Thank you for your assistant.* *Stella Walters* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dont8know8me <@t> yahoo.com Sun Nov 17 02:25:56 2013 From: dont8know8me <@t> yahoo.com (richard peralta) Date: Sun Nov 17 02:57:24 2013 Subject: [Histonet] Hi Histonet! Message-ID: http://cdn.spotcream.com/images/searchxml.php?buhkbv172knmpghk ================ Heard on Noahs' ark: Sailing is fun, but scrubbing the decks is aardvark. From mward <@t> wakehealth.edu Mon Nov 18 08:37:14 2013 From: mward <@t> wakehealth.edu (Martha Ward-Pathology) Date: Mon Nov 18 08:37:22 2013 Subject: [Histonet] IgG sub classes and anti-PLA2R in renals In-Reply-To: <899EAA40AE6E47488CE0637489EFB30C1804A29A@WCU-XCH-03.PASSHE.LCL> References: <899EAA40AE6E47488CE0637489EFB30C1804A29A@WCU-XCH-03.PASSHE.LCL> Message-ID: I am looking for information on IgG sub class antibodies, as well as anti-PLA2R, for use in renal biopsies. Specifically using immunofluorescence. Is anyone using these antibodies routinely in their renal biopsy protocols? If so, would you mind sending me information such as vendors, etc. Actually any information is appreciated. I have just had a inquiry from our renal pathologist about offering these. Thanks in advance for your help. ? Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard ?\? Winston-Salem, NC 27157 p 336.716.2109 ?\? f 336.716.5890 ? mward@wakehealth.edu ? ? ? From ktuttle <@t> umm.edu Mon Nov 18 10:03:18 2013 From: ktuttle <@t> umm.edu (Kimberly Tuttle) Date: Mon Nov 18 10:04:01 2013 Subject: [Histonet] Anyone using Section Transfer System by Microm? Message-ID: <5289F3F60200001A000A1D51@gwia2.umm.edu> Thanks for bringing this to my attention, Ive never heard of it until now. I want one too, it does seem like an asset for microarrays, how much$ do they run? >>> "Morken, Timothy" 11/14/13 11:58 AM >>> Is anyone using the Thermo/Microm Secton Transfer System on their microtome? http://cellab.se/pdfbroschyr/Microm/STS.pdf I'd like to get some input for using it to cut serial sections of microarrays. Thanks! Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center Box 1656 505 Parnassus Ave San Francisco, CA 94143 USA 415.353.1266 (office) tim.morken@ucsfmedctr.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. From Juliana.Montero <@t> usmd.com Mon Nov 18 10:03:48 2013 From: Juliana.Montero <@t> usmd.com (Juliana Montero) Date: Mon Nov 18 10:05:00 2013 Subject: [Histonet] external alarm for Tissue-Tek VIP E300 Message-ID: I am looking to get an external alarm for our tissue processor. Any suggestions? Juliana Montero, HTL Lead Histotechnologist USMD Pathology Laboratory 900 Airport FWY Ste. 132 Hurst, TX 76054 817-576-0590 Office 817-576-0591 Fax 432-238-8981 Cell juliana.montero@usmd.com [USMD] The information contained in this e-mail message is intended exclusively for the recipient(s) named above. This communication may contain information that is confidential, proprietary or otherwise legally exempt from disclosure. If you are not the named addressee, you are not authorized to read, print, retain, copy or disseminate this message or any part of it.If you have received this message in error, please notify the sender immediately by e-mail and delete all copies of the message. Review by any individual other than the intended recipient does not waive a privileged relationship (if applicable) between the sender and the recipient. USMD From candice_camille <@t> yahoo.com Mon Nov 18 10:42:06 2013 From: candice_camille <@t> yahoo.com (Candice Smoots) Date: Mon Nov 18 10:42:12 2013 Subject: [Histonet] Immunohistochemistry for beginners Message-ID: <1384792926.69788.YahooMailNeo@web165001.mail.bf1.yahoo.com> Hi fellow histonetters I have a colleague that is completely new to immunohistochemistry. I have gone over what I could with him, however, I think that some literature would help him more. Do you guys know of any books or articles that could help a beginner like him. I do not have any on hand but would like to offer something. ? ? I remain yours truly, Candice Camille From liz <@t> premierlab.com Mon Nov 18 10:47:32 2013 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Mon Nov 18 10:48:52 2013 Subject: [Histonet] Immunohistochemistry for beginners In-Reply-To: <1384792926.69788.YahooMailNeo@web165001.mail.bf1.yahoo.com> References: <1384792926.69788.YahooMailNeo@web165001.mail.bf1.yahoo.com> Message-ID: <14E2C6176416974295479C64A11CB9AE019C79E01ED3@SBS2K8.premierlab.local> The dako handbook is a great resource, you can download it from the website. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 881-0763 cell (303) 682-9060 fax liz@premierlab.com Ship to address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Candice Smoots [candice_camille@yahoo.com] Sent: Monday, November 18, 2013 9:42 AM To: Histonet Subject: [Histonet] Immunohistochemistry for beginners Hi fellow histonetters I have a colleague that is completely new to immunohistochemistry. I have gone over what I could with him, however, I think that some literature would help him more. Do you guys know of any books or articles that could help a beginner like him. I do not have any on hand but would like to offer something. I remain yours truly, Candice Camille _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PAMarcum <@t> uams.edu Mon Nov 18 10:48:49 2013 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Mon Nov 18 10:48:55 2013 Subject: [Histonet] Immunohistochemistry for beginners In-Reply-To: <1384792926.69788.YahooMailNeo@web165001.mail.bf1.yahoo.com> References: <1384792926.69788.YahooMailNeo@web165001.mail.bf1.yahoo.com> Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA32D316BEE7@Mail2Node2.ad.uams.edu> Dako has some excellent training information for beginners and I think Leica may also have something. If you talk to your Dako rep or contact them by phone or look online you should be able to get the information fairly quickly. Pam Marcum -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Candice Smoots Sent: Monday, November 18, 2013 10:42 AM To: Histonet Subject: [Histonet] Immunohistochemistry for beginners Hi fellow histonetters I have a colleague that is completely new to immunohistochemistry. I have gone over what I could with him, however, I think that some literature would help him more. Do you guys know of any books or articles that could help a beginner like him. I do not have any on hand but would like to offer something. ? ? I remain yours truly, Candice Camille _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From POWELL_SA <@t> mercer.edu Mon Nov 18 11:01:24 2013 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Mon Nov 18 11:01:27 2013 Subject: [Histonet] Immunohistochemistry for beginners In-Reply-To: <41D3A1AF6FEF0643BDC89E0516A6EA32D316BEE7@Mail2Node2.ad.uams.edu> References: <1384792926.69788.YahooMailNeo@web165001.mail.bf1.yahoo.com> <41D3A1AF6FEF0643BDC89E0516A6EA32D316BEE7@Mail2Node2.ad.uams.edu> Message-ID: <9BF995BC0E47744E9673A41486E24EE25845A20487@MERCERMAIL.MercerU.local> I agree with Pam, it helped me tremendously when I was running my IHC reference lab. And I think you may be able to get it online. Shirley -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marcum, Pamela A Sent: Monday, November 18, 2013 11:49 AM To: 'Candice Smoots'; Histonet Subject: RE: [Histonet] Immunohistochemistry for beginners Dako has some excellent training information for beginners and I think Leica may also have something. If you talk to your Dako rep or contact them by phone or look online you should be able to get the information fairly quickly. Pam Marcum -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Candice Smoots Sent: Monday, November 18, 2013 10:42 AM To: Histonet Subject: [Histonet] Immunohistochemistry for beginners Hi fellow histonetters I have a colleague that is completely new to immunohistochemistry. I have gone over what I could with him, however, I think that some literature would help him more. Do you guys know of any books or articles that could help a beginner like him. I do not have any on hand but would like to offer something. ? ? I remain yours truly, Candice Camille _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Mon Nov 18 11:01:32 2013 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Mon Nov 18 11:01:36 2013 Subject: [Histonet] Immunohistochemistry for beginners In-Reply-To: <41D3A1AF6FEF0643BDC89E0516A6EA32D316BEE7@Mail2Node2.ad.uams.edu> References: <1384792926.69788.YahooMailNeo@web165001.mail.bf1.yahoo.com>, <41D3A1AF6FEF0643BDC89E0516A6EA32D316BEE7@Mail2Node2.ad.uams.edu> Message-ID: You used to be able to download the DAKO handbook as a pdf from the website. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: PAMarcum@uams.edu > To: candice_camille@yahoo.com; histonet@lists.utsouthwestern.edu > Date: Mon, 18 Nov 2013 16:48:49 +0000 > Subject: RE: [Histonet] Immunohistochemistry for beginners > CC: > > Dako has some excellent training information for beginners and I think Leica may also have something. If you talk to your Dako rep or contact them by phone or look online you should be able to get the information fairly quickly. > > Pam Marcum > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Candice Smoots > Sent: Monday, November 18, 2013 10:42 AM > To: Histonet > Subject: [Histonet] Immunohistochemistry for beginners > > Hi fellow histonetters > > I have a colleague that is completely new to immunohistochemistry. I have gone over what I could with him, however, I think that some literature would help him more. Do you guys know of any books or articles that could help a beginner like him. I do not have any on hand but would like to offer something. > > > I remain yours truly, > > Candice Camille > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ---------------------------------------------------------------------- > Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From trathborne <@t> somerset-healthcare.com Mon Nov 18 11:04:32 2013 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Mon Nov 18 11:05:54 2013 Subject: [Histonet] RE: external alarm for Tissue-Tek VIP E300 In-Reply-To: References: Message-ID: <3AD061FE740D464FAC7BF6B5CFB75707A8B3C08C@smcmail02.somerset-healthcare.com> Your Sales/Service rep should be able to suggest something. We have one for our VIP and were assisted by the company. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Juliana Montero Sent: Monday, November 18, 2013 11:04 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] external alarm for Tissue-Tek VIP E300 I am looking to get an external alarm for our tissue processor. Any suggestions? Juliana Montero, HTL Lead Histotechnologist USMD Pathology Laboratory 900 Airport FWY Ste. 132 Hurst, TX 76054 817-576-0590 Office 817-576-0591 Fax 432-238-8981 Cell juliana.montero@usmd.com [USMD] The information contained in this e-mail message is intended exclusively for the recipient(s) named above. This communication may contain information that is confidential, proprietary or otherwise legally exempt from disclosure. If you are not the named addressee, you are not authorized to read, print, retain, copy or disseminate this message or any part of it.If you have received this message in error, please notify the sender immediately by e-mail and delete all copies of the message. Review by any individual other than the intended recipient does not waive a privileged relationship (if applicable) between the sender and the recipient. USMD _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From doug.porter <@t> caplab.org Mon Nov 18 11:43:34 2013 From: doug.porter <@t> caplab.org (Douglas Porter) Date: Mon Nov 18 11:40:46 2013 Subject: [Histonet] external alarm for Tissue-Tek VIP E300 In-Reply-To: References: Message-ID: <004801cee485$b4a971f0$1dfc55d0$@caplab.org> We have ours tied into our security system so that when the alarm goes off we get a call from the security company. Works quite well! Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.porter@caplab.org www.caplab.org ? ? The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, copying, forwarding or capture of this communication is strictly prohibited. If you have received this communication in error, please notify me immediately by return e-mail and delete this and all copies. Thank-you. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Juliana Montero Sent: Monday, November 18, 2013 11:04 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] external alarm for Tissue-Tek VIP E300 I am looking to get an external alarm for our tissue processor. Any suggestions? Juliana Montero, HTL Lead Histotechnologist USMD Pathology Laboratory 900 Airport FWY Ste. 132 Hurst, TX 76054 817-576-0590 Office 817-576-0591 Fax 432-238-8981 Cell juliana.montero@usmd.com [USMD] The information contained in this e-mail message is intended exclusively for the recipient(s) named above. This communication may contain information that is confidential, proprietary or otherwise legally exempt from disclosure. If you are not the named addressee, you are not authorized to read, print, retain, copy or disseminate this message or any part of it.If you have received this message in error, please notify the sender immediately by e-mail and delete all copies of the message. Review by any individual other than the intended recipient does not waive a privileged relationship (if applicable) between the sender and the recipient. USMD _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TMcNemar <@t> lmhealth.org Mon Nov 18 11:47:23 2013 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Mon Nov 18 11:47:32 2013 Subject: [Histonet] RE: external alarm for Tissue-Tek VIP E300 In-Reply-To: References: Message-ID: Ours is tied into the main lab system that monitors all of the refrigerators and freezers. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Juliana Montero Sent: Monday, November 18, 2013 11:04 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] external alarm for Tissue-Tek VIP E300 I am looking to get an external alarm for our tissue processor. Any suggestions? Juliana Montero, HTL Lead Histotechnologist USMD Pathology Laboratory 900 Airport FWY Ste. 132 Hurst, TX 76054 817-576-0590 Office 817-576-0591 Fax 432-238-8981 Cell juliana.montero@usmd.com [USMD] The information contained in this e-mail message is intended exclusively for the recipient(s) named above. This communication may contain information that is confidential, proprietary or otherwise legally exempt from disclosure. If you are not the named addressee, you are not authorized to read, print, retain, copy or disseminate this message or any part of it.If you have received this message in error, please notify the sender immediately by e-mail and delete all copies of the message. Review by any individual other than the intended recipient does not waive a privileged relationship (if applicable) between the sender and the recipient. USMD _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From joelleweaver <@t> hotmail.com Mon Nov 18 12:08:43 2013 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Mon Nov 18 12:08:57 2013 Subject: [Histonet] external alarm for Tissue-Tek VIP E300 In-Reply-To: References: Message-ID: Medical Equipment Source LLC Info@medequiosourc.com 724-625-2235 Had some good solutions for this type of issue Joelle Weaver MAOM, HTL (ASCP) QIHC > From: Juliana.Montero@usmd.com > To: histonet@lists.utsouthwestern.edu > Date: Mon, 18 Nov 2013 16:03:48 +0000 > Subject: [Histonet] external alarm for Tissue-Tek VIP E300 > > I am looking to get an external alarm for our tissue processor. Any suggestions? > > > > Juliana Montero, HTL > > Lead Histotechnologist > > > > USMD Pathology Laboratory > > 900 Airport FWY > > Ste. 132 > > Hurst, TX 76054 > > > > 817-576-0590 Office > > 817-576-0591 Fax > > 432-238-8981 Cell > > > > juliana.montero@usmd.com > > > > > > > > [USMD] > > > The information contained in this e-mail message is intended exclusively for the recipient(s) named above. This communication may contain information that is confidential, proprietary or otherwise legally exempt from disclosure. If you are not the named addressee, you are not authorized to read, print, retain, copy or disseminate this message or any part of it.If you have received this message in error, please notify the sender immediately by e-mail and delete all copies of the message. Review by any individual other than the intended recipient does not waive a privileged relationship (if applicable) between the sender and the recipient. > > USMD > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Terri.Brown <@t> Northside.com Mon Nov 18 11:56:46 2013 From: Terri.Brown <@t> Northside.com (Terri Brown) Date: Mon Nov 18 12:10:37 2013 Subject: [Histonet] external alarm for Tissue-Tek VIP E300 In-Reply-To: References: Message-ID: <731941C266951A47BEF11E5EFAAED9C91EF5D3D2@nsmvexch01.northside.local> We use Amega Scientific Corporation and get great service. Terri H. Brown,, HT (ASCP) Pathology Laboratory Manager Northside Hospital Atlanta terri.brown@northside.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Juliana Montero Sent: Monday, November 18, 2013 11:04 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] external alarm for Tissue-Tek VIP E300 I am looking to get an external alarm for our tissue processor. Any suggestions? Juliana Montero, HTL Lead Histotechnologist USMD Pathology Laboratory 900 Airport FWY Ste. 132 Hurst, TX 76054 817-576-0590 Office 817-576-0591 Fax 432-238-8981 Cell juliana.montero@usmd.com [USMD] The information contained in this e-mail message is intended exclusively for the recipient(s) named above. This communication may contain information that is confidential, proprietary or otherwise legally exempt from disclosure. If you are not the named addressee, you are not authorized to read, print, retain, copy or disseminate this message or any part of it.If you have received this message in error, please notify the sender immediately by e-mail and delete all copies of the message. Review by any individual other than the intended recipient does not waive a privileged relationship (if applicable) between the sender and the recipient. USMD _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by Northside Hospital. It may contain information that is confidential, privileged, proprietary, or otherwise legally exempt from disclosure. If you are not the intended recipient, you are hereby notified that you are not authorized to read, print, retain, copy or disseminate this message, any part of it, or any attachments. If you have received this message in error, please delete this message and any attachments from your system without reading the content and notify the sender immediately of the inadvertent transmission. There is no intent on the part of the sender to waive any privilege. From smah2 <@t> msn.com Mon Nov 18 12:14:54 2013 From: smah2 <@t> msn.com (Sheila Haas) Date: Mon Nov 18 12:14:57 2013 Subject: [Histonet] Quality Assurance Message-ID: Hi All.I have recently been given a new position and am now in charge of quality assurance for our entire organization. While I have been involved in many QA projects and have carried out QA throughout histology, I haven't had much experience is looking at QA in its entirety. If anyone has suggestions or training information/guidance, I'd appreciate the help! Thank you, Sheila Haas Micro Path Laboratories, Inc. From Timothy.Morken <@t> ucsfmedctr.org Mon Nov 18 12:33:04 2013 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Mon Nov 18 12:33:19 2013 Subject: [Histonet] Quality Assurance In-Reply-To: References: Message-ID: <761E2B5697F795489C8710BCC72141FF0B2B08@ex07.net.ucsf.edu> Sheila, CAP has a concise QA book called "Quality Management in Anatomic Pathology." They tie in the CAP checklists to each section for easy reference. There is a companion book for clinical laboratory: " Quality Management In Clinical Laboratories: Promoting Patient Safety Through Risk Reduction And Continuous Improvement" Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Haas Sent: Monday, November 18, 2013 10:15 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Quality Assurance Hi All.I have recently been given a new position and am now in charge of quality assurance for our entire organization. While I have been involved in many QA projects and have carried out QA throughout histology, I haven't had much experience is looking at QA in its entirety. If anyone has suggestions or training information/guidance, I'd appreciate the help! Thank you, Sheila Haas Micro Path Laboratories, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Mon Nov 18 13:44:11 2013 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Mon Nov 18 13:44:18 2013 Subject: [Histonet] Quality Assurance In-Reply-To: References: Message-ID: Congratulations Sheila on your new position! Agree with Tim Morken on his suggestion of a book- I have used that one a lot myself just in my regular work ( I'm not specifically assigned to Q/A). Also, If you are going to get into CLIA mod-high complexity tests, I also like the CLSI guidebooks for IHC and ISH. Worth it to check out their website, they have some nicely put together more general QA materials too. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: smah2@msn.com > To: histonet@lists.utsouthwestern.edu > Date: Mon, 18 Nov 2013 18:14:54 +0000 > Subject: [Histonet] Quality Assurance > > Hi All.I have recently been given a new position and am now in charge of quality assurance for our entire organization. > While I have been involved in many QA projects and have carried out QA throughout histology, I haven't > had much experience is looking at QA in its entirety. If anyone has suggestions or training information/guidance, I'd appreciate the help! > > Thank you, > Sheila Haas > Micro Path Laboratories, Inc. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From llang <@t> aipathology.com Mon Nov 18 14:02:04 2013 From: llang <@t> aipathology.com (LeAnn Lang) Date: Mon Nov 18 14:02:12 2013 Subject: [Histonet] Specimen collection/transportation Message-ID: <704247D5A09D004C9E6B115138D1703AB2BC72@hpserv001.aipathology.local> We were recently contacted by our hospital indicating that we are in violation of OSHA by using the process we currently are using. Currently, we provided prefilled 10% neutral buffered formalin containers to the surgical suites, birthing units, etc. They fill the containers with the specimens and return them to the pathology lab. We have done this process for many many years and have never been questioned for this by either CAP or Joint Commission. What is your process for specimen collection/transport? Are the specimens put in formalin in the surgery suites/birthing unit/etc or in the pathology laboratory? How about placentas, are they sent in formalin from the floor or are they put in formalin in the histology lab? Thank you! LeAnn <>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<> LeAnn Lang Associates in Pathology Practice Administrator Phone: 715-847-0075 (ext 50259) llang@aipathology.com From chapcl <@t> yahoo.com Mon Nov 18 14:06:16 2013 From: chapcl <@t> yahoo.com (Will Chappell) Date: Mon Nov 18 14:06:28 2013 Subject: [Histonet] Specimen collection/transportation In-Reply-To: <704247D5A09D004C9E6B115138D1703AB2BC72@hpserv001.aipathology.local> References: <704247D5A09D004C9E6B115138D1703AB2BC72@hpserv001.aipathology.local> Message-ID: This to me seems very odd. Almost exclusively specimens are sent to my lab in formalin. Placentas are usually sent fresh simply because of their size. If anything, the birthing unit may not be in compliance, but it has nothing to do with the lab. The formalin containers must be properly labelled, and appropriate SOPs in use on the floor, usually to include a spill kit. I wrote the procedures for the floor units, but it is their responsibility to be in compliance. Will Chappell, HTL(ASCP) Sent from my iPhone > On Nov 18, 2013, at 12:02 PM, "LeAnn Lang" wrote: > > We were recently contacted by our hospital indicating that we are in > violation of OSHA by using the process we currently are using. > Currently, we provided prefilled 10% neutral buffered formalin > containers to the surgical suites, birthing units, etc. They fill the > containers with the specimens and return them to the pathology lab. We > have done this process for many many years and have never been > questioned for this by either CAP or Joint Commission. What is your > process for specimen collection/transport? Are the specimens put in > formalin in the surgery suites/birthing unit/etc or in the pathology > laboratory? How about placentas, are they sent in formalin from the > floor or are they put in formalin in the histology lab? > > > > Thank you! > > LeAnn > > > > > > <>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<> > > LeAnn Lang > > Associates in Pathology > > Practice Administrator > > Phone: 715-847-0075 (ext 50259) > > llang@aipathology.com > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Wanda.Smith <@t> HCAhealthcare.com Mon Nov 18 14:07:06 2013 From: Wanda.Smith <@t> HCAhealthcare.com (Wanda.Smith@HCAhealthcare.com) Date: Mon Nov 18 14:07:48 2013 Subject: [Histonet] RE: Specimen collection/transportation In-Reply-To: <704247D5A09D004C9E6B115138D1703AB2BC72@hpserv001.aipathology.local> References: <704247D5A09D004C9E6B115138D1703AB2BC72@hpserv001.aipathology.local> Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BA27E66B243D@NADCWPMSGCMS03.hca.corpad.net> What exactly is the violation? WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC? 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LeAnn Lang Sent: Monday, November 18, 2013 3:02 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Specimen collection/transportation We were recently contacted by our hospital indicating that we are in violation of OSHA by using the process we currently are using. Currently, we provided prefilled 10% neutral buffered formalin containers to the surgical suites, birthing units, etc. They fill the containers with the specimens and return them to the pathology lab. We have done this process for many many years and have never been questioned for this by either CAP or Joint Commission. What is your process for specimen collection/transport? Are the specimens put in formalin in the surgery suites/birthing unit/etc or in the pathology laboratory? How about placentas, are they sent in formalin from the floor or are they put in formalin in the histology lab? Thank you! LeAnn <>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<> LeAnn Lang Associates in Pathology Practice Administrator Phone: 715-847-0075 (ext 50259) llang@aipathology.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From trathborne <@t> somerset-healthcare.com Mon Nov 18 14:08:13 2013 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Mon Nov 18 14:09:21 2013 Subject: [Histonet] Specimen collection/transportation In-Reply-To: References: <704247D5A09D004C9E6B115138D1703AB2BC72@hpserv001.aipathology.local> Message-ID: <3AD061FE740D464FAC7BF6B5CFB75707A8B3D1C4@smcmail02.somerset-healthcare.com> Did they state which OSHA standard you were in violation of? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Will Chappell Sent: Monday, November 18, 2013 3:06 PM To: LeAnn Lang Cc: Subject: Re: [Histonet] Specimen collection/transportation This to me seems very odd. Almost exclusively specimens are sent to my lab in formalin. Placentas are usually sent fresh simply because of their size. If anything, the birthing unit may not be in compliance, but it has nothing to do with the lab. The formalin containers must be properly labelled, and appropriate SOPs in use on the floor, usually to include a spill kit. I wrote the procedures for the floor units, but it is their responsibility to be in compliance. Will Chappell, HTL(ASCP) Sent from my iPhone > On Nov 18, 2013, at 12:02 PM, "LeAnn Lang" wrote: > > We were recently contacted by our hospital indicating that we are in > violation of OSHA by using the process we currently are using. > Currently, we provided prefilled 10% neutral buffered formalin > containers to the surgical suites, birthing units, etc. They fill the > containers with the specimens and return them to the pathology lab. We > have done this process for many many years and have never been > questioned for this by either CAP or Joint Commission. What is your > process for specimen collection/transport? Are the specimens put in > formalin in the surgery suites/birthing unit/etc or in the pathology > laboratory? How about placentas, are they sent in formalin from the > floor or are they put in formalin in the histology lab? > > > > Thank you! > > LeAnn > > > > > > <>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<> > > LeAnn Lang > > Associates in Pathology > > Practice Administrator > > Phone: 715-847-0075 (ext 50259) > > llang@aipathology.com > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From llang <@t> aipathology.com Mon Nov 18 14:13:48 2013 From: llang <@t> aipathology.com (LeAnn Lang) Date: Mon Nov 18 14:13:59 2013 Subject: [Histonet] Specimen collection/transportation In-Reply-To: <3AD061FE740D464FAC7BF6B5CFB75707A8B3D1C4@smcmail02.somerset-healthcare.com> References: <704247D5A09D004C9E6B115138D1703AB2BC72@hpserv001.aipathology.local> <3AD061FE740D464FAC7BF6B5CFB75707A8B3D1C4@smcmail02.somerset-healthcare.com> Message-ID: <704247D5A09D004C9E6B115138D1703AB2BC76@hpserv001.aipathology.local> This was the message I received this morning: (I asked for the specific documentation on the violation). I also asked why the OBTs are NOT using precautions when working with the placenta and formalin. Just discovered this am that we are using formalin filled buckets for placentas going to pathology This is a huge safety issue for the staff and an OSHA violation. The standard practice for placentas going to pathology is to store them in a refrigerator and then pathology picks them up. The key component is the elimination of the formalin. Handling of formalin requires safety goggles, chemical resistant gloves and protective clothing, Venting under a hood is also recommended. The OBTs that place the placentas in a bucket of formalin have not been doing any of this or using any precautions. The upshot is if this stuff spills it can cause severe health problems (at the last hospital I was at it was spilled and EVS worker tried to clean it up and was in the ICU for two weeks). -----Original Message----- From: Rathborne, Toni [mailto:trathborne@somerset-healthcare.com] Sent: Monday, November 18, 2013 2:08 PM To: 'Will Chappell'; LeAnn Lang Cc: Subject: RE: [Histonet] Specimen collection/transportation Did they state which OSHA standard you were in violation of? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Will Chappell Sent: Monday, November 18, 2013 3:06 PM To: LeAnn Lang Cc: Subject: Re: [Histonet] Specimen collection/transportation This to me seems very odd. Almost exclusively specimens are sent to my lab in formalin. Placentas are usually sent fresh simply because of their size. If anything, the birthing unit may not be in compliance, but it has nothing to do with the lab. The formalin containers must be properly labelled, and appropriate SOPs in use on the floor, usually to include a spill kit. I wrote the procedures for the floor units, but it is their responsibility to be in compliance. Will Chappell, HTL(ASCP) Sent from my iPhone > On Nov 18, 2013, at 12:02 PM, "LeAnn Lang" wrote: > > We were recently contacted by our hospital indicating that we are in > violation of OSHA by using the process we currently are using. > Currently, we provided prefilled 10% neutral buffered formalin > containers to the surgical suites, birthing units, etc. They fill the > containers with the specimens and return them to the pathology lab. We > have done this process for many many years and have never been > questioned for this by either CAP or Joint Commission. What is your > process for specimen collection/transport? Are the specimens put in > formalin in the surgery suites/birthing unit/etc or in the pathology > laboratory? How about placentas, are they sent in formalin from the > floor or are they put in formalin in the histology lab? > > > > Thank you! > > LeAnn > > > > > > <>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<> > > LeAnn Lang > > Associates in Pathology > > Practice Administrator > > Phone: 715-847-0075 (ext 50259) > > llang@aipathology.com > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From chapcl <@t> yahoo.com Mon Nov 18 14:07:49 2013 From: chapcl <@t> yahoo.com (Will Chappell) Date: Mon Nov 18 14:16:32 2013 Subject: [Histonet] Specimen collection/transportation In-Reply-To: References: <704247D5A09D004C9E6B115138D1703AB2BC72@hpserv001.aipathology.local> Message-ID: And we just passed CAP with zero deficiencies. Sent from my iPhone > On Nov 18, 2013, at 12:06 PM, Will Chappell wrote: > > This to me seems very odd. Almost exclusively specimens are sent to my lab in formalin. Placentas are usually sent fresh simply because of their size. > > If anything, the birthing unit may not be in compliance, but it has nothing to do with the lab. The formalin containers must be properly labelled, and appropriate SOPs in use on the floor, usually to include a spill kit. I wrote the procedures for the floor units, but it is their responsibility to be in compliance. > > Will Chappell, HTL(ASCP) > > Sent from my iPhone > >> On Nov 18, 2013, at 12:02 PM, "LeAnn Lang" wrote: >> >> We were recently contacted by our hospital indicating that we are in >> violation of OSHA by using the process we currently are using. >> Currently, we provided prefilled 10% neutral buffered formalin >> containers to the surgical suites, birthing units, etc. They fill the >> containers with the specimens and return them to the pathology lab. We >> have done this process for many many years and have never been >> questioned for this by either CAP or Joint Commission. What is your >> process for specimen collection/transport? Are the specimens put in >> formalin in the surgery suites/birthing unit/etc or in the pathology >> laboratory? How about placentas, are they sent in formalin from the >> floor or are they put in formalin in the histology lab? >> >> >> >> Thank you! >> >> LeAnn >> >> >> >> >> >> <>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<> >> >> LeAnn Lang >> >> Associates in Pathology >> >> Practice Administrator >> >> Phone: 715-847-0075 (ext 50259) >> >> llang@aipathology.com >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From foreightl <@t> gmail.com Mon Nov 18 14:36:37 2013 From: foreightl <@t> gmail.com (Patrick Laurie) Date: Mon Nov 18 14:36:42 2013 Subject: [Histonet] Specimen collection/transportation In-Reply-To: <704247D5A09D004C9E6B115138D1703AB2BC76@hpserv001.aipathology.local> References: <704247D5A09D004C9E6B115138D1703AB2BC72@hpserv001.aipathology.local> <3AD061FE740D464FAC7BF6B5CFB75707A8B3D1C4@smcmail02.somerset-healthcare.com> <704247D5A09D004C9E6B115138D1703AB2BC76@hpserv001.aipathology.local> Message-ID: Ah, I think that the key thing is that the OBT's are not following procedure and their procedures are in violation of the OSHA regs. If they were to use the proper PPE and instructions, there should not be an issue. Also, make sure that there is some kind of spill procedure/kit available. In my state, only certain people who are trained can clean up any spill that is over 1 gallon. Patrick Laurie On Mon, Nov 18, 2013 at 3:13 PM, LeAnn Lang wrote: > This was the message I received this morning: (I asked for the specific > documentation on the violation). I also asked why the OBTs are NOT > using precautions when working with the placenta and formalin. > > > Just discovered this am that we are using formalin filled > buckets for placentas going to pathology > This is a huge safety issue for the staff and an OSHA violation. > The standard practice for placentas going to pathology is to > store them in a refrigerator and then pathology picks them up. > The key component is the elimination of the formalin. > Handling of formalin requires safety goggles, chemical resistant > gloves and protective clothing, Venting under a hood is also > recommended. > The OBTs that place the placentas in a bucket of formalin have > not been doing any of this or using any precautions. > The upshot is if this stuff spills it can cause severe health > problems (at the last hospital I was at it was spilled and EVS worker > tried to clean it up and was in the ICU for two weeks). > > -----Original Message----- > From: Rathborne, Toni [mailto:trathborne@somerset-healthcare.com] > Sent: Monday, November 18, 2013 2:08 PM > To: 'Will Chappell'; LeAnn Lang > Cc: > Subject: RE: [Histonet] Specimen collection/transportation > > Did they state which OSHA standard you were in violation of? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Will > Chappell > Sent: Monday, November 18, 2013 3:06 PM > To: LeAnn Lang > Cc: > Subject: Re: [Histonet] Specimen collection/transportation > > This to me seems very odd. Almost exclusively specimens are sent to my > lab in formalin. Placentas are usually sent fresh simply because of > their size. > > If anything, the birthing unit may not be in compliance, but it has > nothing to do with the lab. The formalin containers must be properly > labelled, and appropriate SOPs in use on the floor, usually to include a > spill kit. I wrote the procedures for the floor units, but it is their > responsibility to be in compliance. > > Will Chappell, HTL(ASCP) > > Sent from my iPhone > > > On Nov 18, 2013, at 12:02 PM, "LeAnn Lang" > wrote: > > > > We were recently contacted by our hospital indicating that we are in > > violation of OSHA by using the process we currently are using. > > Currently, we provided prefilled 10% neutral buffered formalin > > containers to the surgical suites, birthing units, etc. They fill > the > > containers with the specimens and return them to the pathology lab. > We > > have done this process for many many years and have never been > > questioned for this by either CAP or Joint Commission. What is your > > process for specimen collection/transport? Are the specimens put in > > formalin in the surgery suites/birthing unit/etc or in the pathology > > laboratory? How about placentas, are they sent in formalin from the > > floor or are they put in formalin in the histology lab? > > > > > > > > Thank you! > > > > LeAnn > > > > > > > > > > > > <>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<> > > > > LeAnn Lang > > > > Associates in Pathology > > > > Practice Administrator > > > > Phone: 715-847-0075 (ext 50259) > > > > llang@aipathology.com > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie(HT)ASCP QIHC Histology Manager Celligent Diagnostics, LLC 101 East W.T. Harris Blvd | Suite 1212 | Charlotte, NC 28262 Work: 704-970-3300 Cell: 704-266-0869 From trathborne <@t> somerset-healthcare.com Mon Nov 18 14:38:39 2013 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Mon Nov 18 14:39:55 2013 Subject: [Histonet] Specimen collection/transportation In-Reply-To: References: <704247D5A09D004C9E6B115138D1703AB2BC72@hpserv001.aipathology.local> <3AD061FE740D464FAC7BF6B5CFB75707A8B3D1C4@smcmail02.somerset-healthcare.com> <704247D5A09D004C9E6B115138D1703AB2BC76@hpserv001.aipathology.local> Message-ID: <3AD061FE740D464FAC7BF6B5CFB75707A8B3D25D@smcmail02.somerset-healthcare.com> Another option would be for them to place the placenta in an empty bucket, then add the formalin. This would eliminate the possibility of a splash injury. From: Patrick Laurie [mailto:foreightl@gmail.com] Sent: Monday, November 18, 2013 3:37 PM To: LeAnn Lang Cc: Rathborne, Toni; Will Chappell; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Specimen collection/transportation Ah, I think that the key thing is that the OBT's are not following procedure and their procedures are in violation of the OSHA regs. If they were to use the proper PPE and instructions, there should not be an issue. Also, make sure that there is some kind of spill procedure/kit available. In my state, only certain people who are trained can clean up any spill that is over 1 gallon. Patrick Laurie On Mon, Nov 18, 2013 at 3:13 PM, LeAnn Lang > wrote: This was the message I received this morning: (I asked for the specific documentation on the violation). I also asked why the OBTs are NOT using precautions when working with the placenta and formalin. Just discovered this am that we are using formalin filled buckets for placentas going to pathology This is a huge safety issue for the staff and an OSHA violation. The standard practice for placentas going to pathology is to store them in a refrigerator and then pathology picks them up. The key component is the elimination of the formalin. Handling of formalin requires safety goggles, chemical resistant gloves and protective clothing, Venting under a hood is also recommended. The OBTs that place the placentas in a bucket of formalin have not been doing any of this or using any precautions. The upshot is if this stuff spills it can cause severe health problems (at the last hospital I was at it was spilled and EVS worker tried to clean it up and was in the ICU for two weeks). -----Original Message----- From: Rathborne, Toni [mailto:trathborne@somerset-healthcare.com] Sent: Monday, November 18, 2013 2:08 PM To: 'Will Chappell'; LeAnn Lang Cc: > Subject: RE: [Histonet] Specimen collection/transportation Did they state which OSHA standard you were in violation of? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Will Chappell Sent: Monday, November 18, 2013 3:06 PM To: LeAnn Lang Cc: > Subject: Re: [Histonet] Specimen collection/transportation This to me seems very odd. Almost exclusively specimens are sent to my lab in formalin. Placentas are usually sent fresh simply because of their size. If anything, the birthing unit may not be in compliance, but it has nothing to do with the lab. The formalin containers must be properly labelled, and appropriate SOPs in use on the floor, usually to include a spill kit. I wrote the procedures for the floor units, but it is their responsibility to be in compliance. Will Chappell, HTL(ASCP) Sent from my iPhone > On Nov 18, 2013, at 12:02 PM, "LeAnn Lang" > wrote: > > We were recently contacted by our hospital indicating that we are in > violation of OSHA by using the process we currently are using. > Currently, we provided prefilled 10% neutral buffered formalin > containers to the surgical suites, birthing units, etc. They fill the > containers with the specimens and return them to the pathology lab. We > have done this process for many many years and have never been > questioned for this by either CAP or Joint Commission. What is your > process for specimen collection/transport? Are the specimens put in > formalin in the surgery suites/birthing unit/etc or in the pathology > laboratory? How about placentas, are they sent in formalin from the > floor or are they put in formalin in the histology lab? > > > > Thank you! > > LeAnn > > > > > > <>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<> > > LeAnn Lang > > Associates in Pathology > > Practice Administrator > > Phone: 715-847-0075 (ext 50259) > > llang@aipathology.com > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Patrick Laurie(HT)ASCP QIHC Histology Manager Celligent Diagnostics, LLC 101 East W.T. Harris Blvd | Suite 1212 | Charlotte, NC 28262 Work: 704-970-3300 Cell: 704-266-0869 From llang <@t> aipathology.com Mon Nov 18 14:50:15 2013 From: llang <@t> aipathology.com (LeAnn Lang) Date: Mon Nov 18 14:50:23 2013 Subject: [Histonet] Specimen collection/transportation In-Reply-To: References: <704247D5A09D004C9E6B115138D1703AB2BC72@hpserv001.aipathology.local><3AD061FE740D464FAC7BF6B5CFB75707A8B3D1C4@smcmail02.somerset-healthcare.com><704247D5A09D004C9E6B115138D1703AB2BC76@hpserv001.aipathology.local> Message-ID: <704247D5A09D004C9E6B115138D1703AB2BC83@hpserv001.aipathology.local> That was my first initial reaction too to be honest and that they are trying to put it on us. From: Patrick Laurie [mailto:foreightl@gmail.com] Sent: Monday, November 18, 2013 2:37 PM To: LeAnn Lang Cc: Rathborne, Toni; Will Chappell; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Specimen collection/transportation Ah, I think that the key thing is that the OBT's are not following procedure and their procedures are in violation of the OSHA regs. If they were to use the proper PPE and instructions, there should not be an issue. Also, make sure that there is some kind of spill procedure/kit available. In my state, only certain people who are trained can clean up any spill that is over 1 gallon. Patrick Laurie On Mon, Nov 18, 2013 at 3:13 PM, LeAnn Lang wrote: This was the message I received this morning: (I asked for the specific documentation on the violation). I also asked why the OBTs are NOT using precautions when working with the placenta and formalin. Just discovered this am that we are using formalin filled buckets for placentas going to pathology This is a huge safety issue for the staff and an OSHA violation. The standard practice for placentas going to pathology is to store them in a refrigerator and then pathology picks them up. The key component is the elimination of the formalin. Handling of formalin requires safety goggles, chemical resistant gloves and protective clothing, Venting under a hood is also recommended. The OBTs that place the placentas in a bucket of formalin have not been doing any of this or using any precautions. The upshot is if this stuff spills it can cause severe health problems (at the last hospital I was at it was spilled and EVS worker tried to clean it up and was in the ICU for two weeks). -----Original Message----- From: Rathborne, Toni [mailto:trathborne@somerset-healthcare.com] Sent: Monday, November 18, 2013 2:08 PM To: 'Will Chappell'; LeAnn Lang Cc: Subject: RE: [Histonet] Specimen collection/transportation Did they state which OSHA standard you were in violation of? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Will Chappell Sent: Monday, November 18, 2013 3:06 PM To: LeAnn Lang Cc: Subject: Re: [Histonet] Specimen collection/transportation This to me seems very odd. Almost exclusively specimens are sent to my lab in formalin. Placentas are usually sent fresh simply because of their size. If anything, the birthing unit may not be in compliance, but it has nothing to do with the lab. The formalin containers must be properly labelled, and appropriate SOPs in use on the floor, usually to include a spill kit. I wrote the procedures for the floor units, but it is their responsibility to be in compliance. Will Chappell, HTL(ASCP) Sent from my iPhone > On Nov 18, 2013, at 12:02 PM, "LeAnn Lang" wrote: > > We were recently contacted by our hospital indicating that we are in > violation of OSHA by using the process we currently are using. > Currently, we provided prefilled 10% neutral buffered formalin > containers to the surgical suites, birthing units, etc. They fill the > containers with the specimens and return them to the pathology lab. We > have done this process for many many years and have never been > questioned for this by either CAP or Joint Commission. What is your > process for specimen collection/transport? Are the specimens put in > formalin in the surgery suites/birthing unit/etc or in the pathology > laboratory? How about placentas, are they sent in formalin from the > floor or are they put in formalin in the histology lab? > > > > Thank you! > > LeAnn > > > > > > <>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<> > > LeAnn Lang > > Associates in Pathology > > Practice Administrator > > Phone: 715-847-0075 (ext 50259) > > llang@aipathology.com > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Patrick Laurie(HT)ASCP QIHC Histology Manager Celligent Diagnostics, LLC 101 East W.T. Harris Blvd | Suite 1212 | Charlotte, NC 28262 Work: 704-970-3300 Cell: 704-266-0869 From LStadler <@t> cbiolabs.com Mon Nov 18 15:05:51 2013 From: LStadler <@t> cbiolabs.com (Lyn Stadler) Date: Mon Nov 18 15:06:04 2013 Subject: [Histonet] Fee For Service Message-ID: <98CC14B915EBA84B9A326D45CC3C1DEC01E510558F@cbiolabs05.CBiolabs.local> A question for clinical lab managers/supervisors: How do you calculate the fee charged for a standard histology test from fixation to result? What things do you factor in? Labor, supplies, overhead,etc? Any and all information would be great! Thanks, Lyn M. Stadler, BS, HTL(ASCP)CM Research Technician Department of Histopathology Buffalo Biolabs, Inc. 73 High Street Buffalo, NY 14203 716-849-6817, ext 417 This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information. From rjbuesa <@t> yahoo.com Mon Nov 18 15:39:03 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Nov 18 15:39:10 2013 Subject: [Histonet] Fee For Service In-Reply-To: <98CC14B915EBA84B9A326D45CC3C1DEC01E510558F@cbiolabs05.CBiolabs.local> References: <98CC14B915EBA84B9A326D45CC3C1DEC01E510558F@cbiolabs05.CBiolabs.local> Message-ID: <1384810743.18340.YahooMailNeo@web163106.mail.bf1.yahoo.com> Yes, all of that and some profit as well. Ren? J. ________________________________ From: Lyn Stadler To: "histonet@lists.utsouthwestern.edu" Sent: Monday, November 18, 2013 4:05 PM Subject: [Histonet] Fee For Service A question for clinical lab managers/supervisors: How do you calculate the fee charged for a standard histology test from fixation to result?? What things do you factor in?? Labor, supplies, overhead,etc? Any and all information would be great! Thanks, Lyn M. Stadler, BS, HTL(ASCP)CM Research Technician Department of Histopathology Buffalo Biolabs, Inc. 73 High Street Buffalo, NY 14203 716-849-6817, ext 417 This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Mon Nov 18 15:40:39 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Nov 18 15:40:46 2013 Subject: [Histonet] Immunohistochemistry for beginners In-Reply-To: <1384792926.69788.YahooMailNeo@web165001.mail.bf1.yahoo.com> References: <1384792926.69788.YahooMailNeo@web165001.mail.bf1.yahoo.com> Message-ID: <1384810839.50394.YahooMailNeo@web163101.mail.bf1.yahoo.com> The best source for such a beginner (and for some "seasoned" as well) is the DAKO manual. Ren? J. ________________________________ From: Candice Smoots To: Histonet Sent: Monday, November 18, 2013 11:42 AM Subject: [Histonet] Immunohistochemistry for beginners Hi fellow histonetters I have a colleague that is completely new to immunohistochemistry. I have gone over what I could with him, however, I think that some literature would help him more. Do you guys know of any books or articles that could help a beginner like him. I do not have any on hand but would like to offer something. ? ? I remain yours truly, Candice Camille _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CObregon <@t> mhs.net Mon Nov 18 15:49:49 2013 From: CObregon <@t> mhs.net (Obregon, Cecilia) Date: Mon Nov 18 15:49:54 2013 Subject: [Histonet] AA Amyloidosis Message-ID: <598BB4A3A92D7F4DAA14C30E4AE70C2F27BC8332@MHSEXMB05.mhs.net> Histoneters, Does anyone have a positive block (or two) for AA Amyloidosis? I'm willing to trade for other hard to find controls. Let me know. Thank you, Cecilia M Obregon Memorial Regional Hospital 3501 Johnsonn Street Hollywood, FL 33021 CONFIDENTIALITY NOTICE: DO NOT FORWARD THIS MESSAGE TO OTHERS WITHOUT PERMISSION OF THE SENDER. This e-mail, including any attachments, may contain confidential or privileged material that is exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, dissemination, copying, or taking any action in reliance on its contents is prohibited. If you have any reason to believe this e-mail was not intended for you, please delete the e-mail and any attachments, and notify the sender immediately. From LSebree <@t> uwhealth.org Mon Nov 18 16:09:02 2013 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Mon Nov 18 16:09:05 2013 Subject: [Histonet] RE: AA Amyloidosis In-Reply-To: <598BB4A3A92D7F4DAA14C30E4AE70C2F27BC8332@MHSEXMB05.mhs.net> References: <598BB4A3A92D7F4DAA14C30E4AE70C2F27BC8332@MHSEXMB05.mhs.net> Message-ID: <77DD817201982748BC67D7960F2F76AF071125@UWHC-MBX12.uwhis.hosp.wisc.edu> We sent part of what we had to ProPath so if you need a reference lab to send to, they offer this IHC test. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Obregon, Cecilia Sent: Monday, November 18, 2013 3:50 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] AA Amyloidosis Histoneters, Does anyone have a positive block (or two) for AA Amyloidosis? I'm willing to trade for other hard to find controls. Let me know. Thank you, Cecilia M Obregon Memorial Regional Hospital 3501 Johnsonn Street Hollywood, FL 33021 CONFIDENTIALITY NOTICE: DO NOT FORWARD THIS MESSAGE TO OTHERS WITHOUT PERMISSION OF THE SENDER. This e-mail, including any attachments, may contain confidential or privileged material that is exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, dissemination, copying, or taking any action in reliance on its contents is prohibited. If you have any reason to believe this e-mail was not intended for you, please delete the e-mail and any attachments, and notify the sender immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Tue Nov 19 07:13:47 2013 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Tue Nov 19 07:13:53 2013 Subject: [Histonet] Specimen collection/transportation In-Reply-To: <704247D5A09D004C9E6B115138D1703AB2BC83@hpserv001.aipathology.local> References: <704247D5A09D004C9E6B115138D1703AB2BC72@hpserv001.aipathology.local><3AD061FE740D464FAC7BF6B5CFB75707A8B3D1C4@smcmail02.somerset-healthcare.com><704247D5A09D004C9E6B115138D1703AB2BC76@hpserv001.aipathology.local>, , <704247D5A09D004C9E6B115138D1703AB2BC83@hpserv001.aipathology.local> Message-ID: The usual procedure where I have worked is that the placenta is delivered fresh to pathology in a large container, or refrigerated until next specimen pick up and formalin is added by the lab staff ( who have PPE, formalin training, spill kit) etc. Joelle Weaver MAOM, HTL (ASCP) QIHC > Date: Mon, 18 Nov 2013 14:50:15 -0600 > From: llang@aipathology.com > To: foreightl@gmail.com > Subject: RE: [Histonet] Specimen collection/transportation > CC: histonet@lists.utsouthwestern.edu > > That was my first initial reaction too to be honest and that they are > trying to put it on us. > > > > From: Patrick Laurie [mailto:foreightl@gmail.com] > Sent: Monday, November 18, 2013 2:37 PM > To: LeAnn Lang > Cc: Rathborne, Toni; Will Chappell; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Specimen collection/transportation > > > > Ah, I think that the key thing is that the OBT's are not following > procedure and their procedures are in violation of the OSHA regs. If > they were to use the proper PPE and instructions, there should not be an > issue. Also, make sure that there is some kind of spill procedure/kit > available. In my state, only certain people who are trained can clean > up any spill that is over 1 gallon. > > > > Patrick Laurie > > > > On Mon, Nov 18, 2013 at 3:13 PM, LeAnn Lang > wrote: > > This was the message I received this morning: (I asked for the specific > documentation on the violation). I also asked why the OBTs are NOT > using precautions when working with the placenta and formalin. > > > Just discovered this am that we are using formalin filled > buckets for placentas going to pathology > This is a huge safety issue for the staff and an OSHA violation. > The standard practice for placentas going to pathology is to > store them in a refrigerator and then pathology picks them up. > The key component is the elimination of the formalin. > Handling of formalin requires safety goggles, chemical resistant > gloves and protective clothing, Venting under a hood is also > recommended. > The OBTs that place the placentas in a bucket of formalin have > not been doing any of this or using any precautions. > The upshot is if this stuff spills it can cause severe health > problems (at the last hospital I was at it was spilled and EVS worker > tried to clean it up and was in the ICU for two weeks). > > > -----Original Message----- > From: Rathborne, Toni [mailto:trathborne@somerset-healthcare.com] > Sent: Monday, November 18, 2013 2:08 PM > To: 'Will Chappell'; LeAnn Lang > Cc: > > Subject: RE: [Histonet] Specimen collection/transportation > > Did they state which OSHA standard you were in violation of? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Will > Chappell > Sent: Monday, November 18, 2013 3:06 PM > To: LeAnn Lang > Cc: > Subject: Re: [Histonet] Specimen collection/transportation > > This to me seems very odd. Almost exclusively specimens are sent to my > lab in formalin. Placentas are usually sent fresh simply because of > their size. > > If anything, the birthing unit may not be in compliance, but it has > nothing to do with the lab. The formalin containers must be properly > labelled, and appropriate SOPs in use on the floor, usually to include a > spill kit. I wrote the procedures for the floor units, but it is their > responsibility to be in compliance. > > Will Chappell, HTL(ASCP) > > Sent from my iPhone > > > On Nov 18, 2013, at 12:02 PM, "LeAnn Lang" > wrote: > > > > We were recently contacted by our hospital indicating that we are in > > violation of OSHA by using the process we currently are using. > > Currently, we provided prefilled 10% neutral buffered formalin > > containers to the surgical suites, birthing units, etc. They fill > the > > containers with the specimens and return them to the pathology lab. > We > > have done this process for many many years and have never been > > questioned for this by either CAP or Joint Commission. What is your > > process for specimen collection/transport? Are the specimens put in > > formalin in the surgery suites/birthing unit/etc or in the pathology > > laboratory? How about placentas, are they sent in formalin from the > > floor or are they put in formalin in the histology lab? > > > > > > > > Thank you! > > > > LeAnn > > > > > > > > > > > > <>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<> > > > > LeAnn Lang > > > > Associates in Pathology > > > > Practice Administrator > > > > Phone: 715-847-0075 (ext 50259) > > > > llang@aipathology.com > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > -- > > Patrick Laurie(HT)ASCP QIHC > > Histology Manager > > Celligent Diagnostics, LLC > > 101 East W.T. Harris Blvd | Suite 1212 | Charlotte, NC 28262 > > Work: 704-970-3300 Cell: 704-266-0869 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mward <@t> wakehealth.edu Tue Nov 19 07:31:17 2013 From: mward <@t> wakehealth.edu (Martha Ward-Pathology) Date: Tue Nov 19 07:31:28 2013 Subject: [Histonet] Vendor suggestions for IgG subclass antibodies In-Reply-To: <731941C266951A47BEF11E5EFAAED9C91EF5D3D2@nsmvexch01.northside.local> References: <731941C266951A47BEF11E5EFAAED9C91EF5D3D2@nsmvexch01.northside.local> Message-ID: I am looking for a vendor for FITC labeled IgG1, IgG2 IgG3 and IgG4 antibodies for use in our immunofluorescence panels when staining renal biopsies. We get most of our FITC antibodies for our panel from Dako but I didn't see these others. Any suggestions are appreciated. Thanks in advance for your help! ? Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard ?\? Winston-Salem, NC 27157 p 336.716.2109 ?\? f 336.716.5890 ? mward@wakehealth.edu ? ? ? From hans <@t> histologistics.com Tue Nov 19 09:16:33 2013 From: hans <@t> histologistics.com (Hans B Snyder) Date: Tue Nov 19 09:16:38 2013 Subject: [Histonet] Methyl Carnoy's processing Message-ID: Hello, I have a researcher who has fixed their specimens in methyl Carnoy's but would like to skip the methyl benzoate step before processing (we do not have methyl benzoate). Does anyone have another method that works well without the methyl benzoate? Thank you Hans B Snyder Histologistics 60 Prescott Street Worcester, MA 01605 508-308-7800 hans@histologistics.com From Finlay.Finlay <@t> glasgow.ac.uk Tue Nov 19 09:29:00 2013 From: Finlay.Finlay <@t> glasgow.ac.uk (Finlay Finlay) Date: Tue Nov 19 09:29:07 2013 Subject: [Histonet] Leica RM2255 block clamp Message-ID: Hello Hoping someone might be able to help with this microtome issue. I have a problem with the block clamp on one of my Leica RM2255's. It has recently loosened up and it is now possible to move the clamped cassette from side-to-side when the clamp is shut. When I turn the bolt on the underside of the clamp it neither tightens up nor loosens off so there does not seem to be a way of taking the clamp apart any further. Leica have suggested that I buy a new block clamp (?292) but it just feels like something that could be easily fixed, perhaps with a new spring? Has anyone had experience with this problem? Thank you Finlay Finlay Histology Technician Forensic Medicine and Science School of Medicine College of Medical, Veterinary and Life Sciences University of Glasgow Joseph Black building Direct Line: +44 (0) 141 3303443 E-mail: finlay.finlay@glasgow.ac.uk The University of Glasgow Charity Number SC004401 From LMurphy2 <@t> aultman.com Tue Nov 19 10:31:35 2013 From: LMurphy2 <@t> aultman.com (Leann M. Murphy) Date: Tue Nov 19 10:31:52 2013 Subject: [Histonet] New CPT code for IHC Message-ID: How will everyone be handling the new CPT code 88343 for IHC going into effect 1/1/14? The CPT code 88342 has a new description stating it is to be used for the "first" antibody and 88343 is to be used for each additional separately identifiable antibody. The concern is that each antibody at any point and time could be the primary or additional. LeAnn Murphy Aultman Hospital Technical Specialist Lmurphy2@aultman.com From chapcl <@t> yahoo.com Tue Nov 19 10:35:12 2013 From: chapcl <@t> yahoo.com (Will Chappell) Date: Tue Nov 19 10:35:29 2013 Subject: [Histonet] New CPT code for IHC In-Reply-To: References: Message-ID: <08CC3423-4D6F-4703-8D16-9FEF1C45BC32@yahoo.com> 88343 refers to subsequent antibodies placed on the same slide such as multiplex staining or the PIN-4. Hope that helps. Will Chappell, HTL(ASCP), QIHC Sent from my iPhone > On Nov 19, 2013, at 8:31 AM, "Leann M. Murphy" wrote: > > How will everyone be handling the new CPT code 88343 for IHC going into effect 1/1/14? The CPT code 88342 has a new description stating it is to be used for the "first" antibody and 88343 is to be used for each additional separately identifiable antibody. The concern is that each antibody at any point and time could be the primary or additional. > > LeAnn Murphy > Aultman Hospital > Technical Specialist > Lmurphy2@aultman.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From catherinesimonson <@t> gmail.com Tue Nov 19 11:08:56 2013 From: catherinesimonson <@t> gmail.com (Catherine Simonson) Date: Tue Nov 19 11:09:01 2013 Subject: [Histonet] omnimap/chromomap kit for ventana Message-ID: Hello Fellow Histonetters, I find myself in a bit of a bind. I have a couple of urgent studies/cases that need to be done by the end of this week, but our kit has run out and is on back-order from Ventana. I need to do Caspase and Major Basic Protein (both rabbit antibodies). Would it be the acme of foolishness to ask if anyone would have a spare kit? Or any other suggestions? is it possible to do antigen retrieval on the machine and use the dead volume in the containers to do the rest of the stain by hand? Thank you all in advance, Catherine Simonson, HT The Jackson Laboratory Bar Harbor, ME From manderson <@t> biocare.net Tue Nov 19 11:10:28 2013 From: manderson <@t> biocare.net (Murray Anderson) Date: Tue Nov 19 11:10:36 2013 Subject: [Histonet] New CPT code for IHC Message-ID: I think that everyone is waiting to see if CMS makes a "policy Change" to accommodate 88342 +88343 code changes. The first indication may be "via the 2014 Medicare Physician Fee Schedule final rule due out for release on or before November 27, or through the 2014 version of the NCCI Policy Manual, which could be available as early as December 1" (care of Dennis Padget). -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Leann M. Murphy Sent: Tuesday, November 19, 2013 8:32 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] New CPT code for IHC How will everyone be handling the new CPT code 88343 for IHC going into effect 1/1/14? The CPT code 88342 has a new description stating it is to be used for the "first" antibody and 88343 is to be used for each additional separately identifiable antibody. The concern is that each antibody at any point and time could be the primary or additional. LeAnn Murphy Aultman Hospital Technical Specialist Lmurphy2@aultman.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ruio7 <@t> hotmail.com Tue Nov 19 11:40:48 2013 From: ruio7 <@t> hotmail.com (Rui TAHARA) Date: Tue Nov 19 11:40:52 2013 Subject: [Histonet] microtome Message-ID: Hello, I have undecalcified biological sample embedded in plastic media (MMA). I am looking for faclities that offers self-service microtome (for plastic embedding sectioining) or short time rental microtome with some training around Quebec, Ontraio, NY. I have been trying to find ones in Montreal, however, its been difficult to find self-service microtome. I appreciate if you would provide some information for this. Thank you in advance, Rui From cebass <@t> buffalo.edu Tue Nov 19 11:43:13 2013 From: cebass <@t> buffalo.edu (Bass, Caroline) Date: Tue Nov 19 11:43:18 2013 Subject: [Histonet] problem with double IF of brain section Message-ID: <3CCEE4FB-40B5-49A2-8456-72D71F89304B@buffalo.edu> Hello everyone, I?m trying a double stain for GFP and tyrosine hydroxylase in floating brain sections. Rat, perfused with formalin. I?m having a big problem, in that it?s clear that the top side of the sections are well stained, as are the bottom, but everything in between is not. These sections are 50 microns. Any suggestions? I assume it?s a penetration problem. But I?m not very comfortable with IF in general. Here?s what my tech does: Placed sections each in a well of a 24 well dish and rinsed in PBS (to remove the Na azide), 1 X 5 min. Rinsed in PBS-0.5% Triton X-100, 3 X 10 min. Incubated tissue in PBST (0.25% Triton X) + 5% NGS, 1 X 60 to 120 min. Incubated sections in 1ary antibody in PBS-T(0.25%) overnight @40C (covered to minimized evaporation). Both primaries together. Tyrosine Hydroxylase 1: 4,000 ImmunoStar #22941 (mouse) a- GFP 1:2,000 Invitrogen 6455 (rabbit) Next day, rinsed tissue w/ PBS-T, 3 x 10 min. Incubated w/ 2ary ab : Alexa 555 donkey anti mouse 1:4,000, Alexa 488 goat anti rabbit, 1:2,000, 1 x 2hs @RT. 2ary ab diluted in PBS. Rinsed w/ PBS, 3 x10 min. Mounted on slides, coverslipped using ProLOng Gold w/ DAPI. Cured in dark place @ RT overnight. Thanks, Caroline From Lynn.Burton <@t> Illinois.gov Tue Nov 19 11:52:18 2013 From: Lynn.Burton <@t> Illinois.gov (Burton, Lynn) Date: Tue Nov 19 11:52:29 2013 Subject: [Histonet] omnimap/chromomap kit for ventana In-Reply-To: References: Message-ID: I have two chromo map kits for the Discovery I can send you. Lynn M Burton Histology Animal Disease Lab Galesburg, Il 309-344-2451 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Catherine Simonson Sent: Tuesday, November 19, 2013 11:09 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] omnimap/chromomap kit for ventana Hello Fellow Histonetters, I find myself in a bit of a bind. I have a couple of urgent studies/cases that need to be done by the end of this week, but our kit has run out and is on back-order from Ventana. I need to do Caspase and Major Basic Protein (both rabbit antibodies). Would it be the acme of foolishness to ask if anyone would have a spare kit? Or any other suggestions? is it possible to do antigen retrieval on the machine and use the dead volume in the containers to do the rest of the stain by hand? Thank you all in advance, Catherine Simonson, HT The Jackson Laboratory Bar Harbor, ME _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From catherinesimonson <@t> gmail.com Tue Nov 19 12:41:51 2013 From: catherinesimonson <@t> gmail.com (Catherine Simonson) Date: Tue Nov 19 12:41:55 2013 Subject: [Histonet] Thank you all. Message-ID: I wanted to thank everybody, especially Lynn Burton, for their help during my time of crisis. It is nice to be a part of a group that is so supportive and willing to lend a hand. Again, thank you. Catherine From Joyce.Weems <@t> emoryhealthcare.org Tue Nov 19 13:12:18 2013 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Tue Nov 19 13:12:26 2013 Subject: [Histonet] Thank you all. In-Reply-To: References: Message-ID: Searching for the "like" button!! We do have an awesome group! Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Catherine Simonson Sent: Tuesday, November 19, 2013 1:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Thank you all. I wanted to thank everybody, especially Lynn Burton, for their help during my time of crisis. It is nice to be a part of a group that is so supportive and willing to lend a hand. Again, thank you. Catherine _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From making <@t> ufl.edu Tue Nov 19 13:30:13 2013 From: making <@t> ufl.edu (King,Michael A) Date: Tue Nov 19 13:30:17 2013 Subject: [Histonet] problem with double IF of brain section Message-ID: <2f45ec3451d0eaf97809303b4f4a88a8@ufl.edu> Caroline, Overnight at 4 degrees may not be long enough for equilibration of the antibody binding reaction deep in the section. Try room temp or longer time if cold is important. The early users of IHC often used 4 days at 4 deg C, based on antibody binding kinetics data. I get full-thickness labeling assessed by confocal microscopy with overnight incubations (both primary and secondary) at room temp in 50 um rodent brain sections. Your secondary incubation may be too short for deep labeling--easy to test. Alternatively, the primary may be depleted (all bound to tissue epitopes) before penetrating the section interior. Increasing the volume or concentration of primary would help. Some 24-well plates (culture-treated) will absorb significant (and variable) amounts of antibody, and little critters can gobble it up too. If you're not using peroxidase reporters you may want to leave azide or another antimicrobial in the incubation solutions. They don't alter fluorescence, though you'll want to convince yourself of this empirically. ---- Message: 12 Date: Tue, 19 Nov 2013 17:43:13 +0000 From: "Bass, Caroline" Subject: [Histonet] problem with double IF of brain section To: Histonet Message-ID: <3CCEE4FB-40B5-49A2-8456-72D71F89304B@buffalo.edu> Content-Type: text/plain; charset="Windows-1252" Hello everyone, I?m trying a double stain for GFP and tyrosine hydroxylase in floating brain sections. Rat, perfused with formalin. I?m having a big problem, in that it?s clear that the top side of the sections are well stained, as are the bottom, but everything in between is not. These sections are 50 microns. Any suggestions? I assume it?s a penetration problem. But I?m not very comfortable with IF in general. Here?s what my tech does: Placed sections each in a well of a 24 well dish and rinsed in PBS (to remove the Na azide), 1 X 5 min. Rinsed in PBS-0.5% Triton X-100, 3 X 10 min. Incubated tissue in PBST (0.25% Triton X) + 5% NGS, 1 X 60 to 120 min. Incubated sections in 1ary antibody in PBS-T(0.25%) overnight @40C (covered to minimized evaporation). Both primaries together. Tyrosine Hydroxylase 1: 4,000 ImmunoStar #22941 (mouse) a- GFP 1:2,000 Invitrogen 6455 (rabbit) Next day, rinsed tissue w/ PBS-T, 3 x 10 min. Incubated w/ 2ary ab : Alexa 555 donkey anti mouse 1:4,000, Alexa 488 goat anti rabbit, 1:2,000, 1 x 2hs @RT. 2ary ab diluted in PBS. Rinsed w/ PBS, 3 x10 min. Mounted on slides, coverslipped using ProLOng Gold w/ DAPI. Cured in dark place @ RT overnight. Thanks, Caroline From patpxs <@t> gmail.com Tue Nov 19 16:23:16 2013 From: patpxs <@t> gmail.com (Paula Sicurello) Date: Tue Nov 19 16:23:21 2013 Subject: [Histonet] -80 freezer question Message-ID: Dear Netters, What is the rationale for storing frozen patient samples at -80? Is there a range that keeps the sample viable but not as cold? Our -80 (which we just inherited) is struggling and I would like to raise the temperature a smidge to ease the stress on the compressor. Thanks! Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 HIPAA Privacy Notification: This message and any accompanying documents are covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, and contain information intended for the specific individual (s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying or the taking of any action based on the contents of this information is strictly prohibited . If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From patpxs <@t> gmail.com Wed Nov 20 08:25:54 2013 From: patpxs <@t> gmail.com (Paula Sicurello) Date: Wed Nov 20 08:25:57 2013 Subject: [Histonet] -80 degrees rationale Message-ID: Hello My Fellow Listers, The question of the day is: What is the rationale for storing frozen biopsies at -80 degrees? I have seen protocols that range in temperature from -40 to -80 degrees. Was -80 selected because that was the lowest freezers could go back in the day? Awaiting your chilly responses! Thanks in advance, Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 HIPAA Privacy Notification: This message and any accompanying documents are covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, and contain information intended for the specific individual (s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying or the taking of any action based on the contents of this information is strictly prohibited . If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From wdesalvo.cac <@t> outlook.com Wed Nov 20 09:58:42 2013 From: wdesalvo.cac <@t> outlook.com (WILLIAM DESALVO) Date: Wed Nov 20 09:58:47 2013 Subject: [Histonet] -80 degrees rationale In-Reply-To: References: Message-ID: Here is my stab at why -80 C. Temperatures between 0?C and ?25?C, the enzymatic activity of cells is only slowed but remains active. Below ?40?C physiochemical exchanges are frozen. Cellular morphology is preserved at -80?C. Shelf life of tissue increases as the temperature drops. Once you get below -80?C you will need cryoprotectors and when you use cryoprotectors, temperatures must be below ?130?C and will go as low as ?196?C (liquid nitrogen). Antibodies and proteins in solution are stable at ?20?C. William DeSalvo, BS HTL(ASCP) > Date: Wed, 20 Nov 2013 09:25:54 -0500 > From: patpxs@gmail.com > To: histonet@lists.utsouthwestern.edu; Microscopy@microscopy.com > CC: > Subject: [Histonet] -80 degrees rationale > > Hello My Fellow Listers, > > The question of the day is: What is the rationale for storing frozen > biopsies at -80 degrees? > > I have seen protocols that range in temperature from -40 to -80 degrees. > > Was -80 selected because that was the lowest freezers could go back in the > day? > > Awaiting your chilly responses! > > Thanks in advance, > > Paula > > -- > Paula Sicurello, HTL (ASCP) > Supervisor, Clinical Electron Microscopy Laboratory > Duke University Health System > Rm.#251M, Duke South, Green Zone > Durham, North Carolina 27710 > P: 919.684.2091 > > HIPAA Privacy Notification: This message and any accompanying documents are > covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, > and contain information intended for the specific individual (s) only. This > information is confidential. If you are not the intended recipient or an > agent responsible for delivering it to the intended recipient, you are > hereby notified that you have received this document in error and that any > review, dissemination, copying or the taking of any action based on the > contents of this information is strictly prohibited . If you have received > this communication in error, please notify us immediately by e-mail, and > delete the original message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Vickroy.Jim <@t> mhsil.com Wed Nov 20 10:03:45 2013 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Wed Nov 20 10:03:51 2013 Subject: [Histonet] Specimens from surigical units Message-ID: We are a large pathology department and process over 35,000 surgical accessions each year. I am interested in learning how other pathology departments keep track of the specimens that are removed in surgery to make sure they are received in pathology. Obviously all specimens received by pathology are entered into the pathology accessioning system however the question has come up to how do we know that every specimen taken in surgery for pathology is actually received in pathology. A couple of our surgical units utilize volunteer couriers since the surgical units are not near pathology. A couple of other units like special procedures and cardiac surgery have a simple notebook that is a signoff when the specimens are taken to pathology, however from there is no tracking system from the main OR or outpatient surgery. In otherwords a specimen could be removed in the OR that was supposed to be sent to pathology does not arrive in pathology. Obviously this doesn't happen often but we are trying to close the potential for an occurrence. Specimens removed in surgery are logged into the surgical OR report however there is no current way for us to track that everything removed that day that was supposed to go to pathology actually made it to pathology. Any ideas? Jim Vickroy James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From PAMarcum <@t> uams.edu Wed Nov 20 10:06:06 2013 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Wed Nov 20 10:06:15 2013 Subject: [Histonet] -80 degrees rationale In-Reply-To: References: Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA32D316C31A@Mail2Node2.ad.uams.edu> Also we had time when finding a non-defrost freezer was difficult and lower temps were not only best; they were about the only way not to have unit turn off and on allowing tissue and antibodies to partially defrost. Some of us learned that the hard way in early days of IHC. Bill is correct in his answer about why it should be stored at the various temps also. Pam Marcum -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO Sent: Wednesday, November 20, 2013 9:59 AM To: Paula Sicurello; histonet; Microscopy@microscopy.com Subject: RE: [Histonet] -80 degrees rationale Here is my stab at why -80 C. Temperatures between 0?C and ?25?C, the enzymatic activity of cells is only slowed but remains active. Below ?40?C physiochemical exchanges are frozen. Cellular morphology is preserved at -80?C. Shelf life of tissue increases as the temperature drops. Once you get below -80?C you will need cryoprotectors and when you use cryoprotectors, temperatures must be below ?130?C and will go as low as ?196?C (liquid nitrogen). Antibodies and proteins in solution are stable at ?20?C. William DeSalvo, BS HTL(ASCP) > Date: Wed, 20 Nov 2013 09:25:54 -0500 > From: patpxs@gmail.com > To: histonet@lists.utsouthwestern.edu; Microscopy@microscopy.com > CC: > Subject: [Histonet] -80 degrees rationale > > Hello My Fellow Listers, > > The question of the day is: What is the rationale for storing frozen > biopsies at -80 degrees? > > I have seen protocols that range in temperature from -40 to -80 degrees. > > Was -80 selected because that was the lowest freezers could go back in > the day? > > Awaiting your chilly responses! > > Thanks in advance, > > Paula > > -- > Paula Sicurello, HTL (ASCP) > Supervisor, Clinical Electron Microscopy Laboratory Duke University > Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina > 27710 > P: 919.684.2091 > > HIPAA Privacy Notification: This message and any accompanying > documents are covered by the Electronic Communications Privacy Act, 18 > U.S.C. 2510-2521, and contain information intended for the specific > individual (s) only. This information is confidential. If you are not > the intended recipient or an agent responsible for delivering it to > the intended recipient, you are hereby notified that you have received > this document in error and that any review, dissemination, copying or > the taking of any action based on the contents of this information is > strictly prohibited . If you have received this communication in > error, please notify us immediately by e-mail, and delete the original message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From mucram11 <@t> comcast.net Wed Nov 20 10:07:55 2013 From: mucram11 <@t> comcast.net (Pam Marcum) Date: Wed Nov 20 10:08:13 2013 Subject: [Histonet] -80 degrees rationale In-Reply-To: Message-ID: <393730792.1909544.1384963675637.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Also we had time when finding a non-defrost freezer was difficult and lower temps were not only best; they were about the only way not to have unit turn off and on allowing tissue and antibodies to partially defrost. ? Some of us learned that the hard way in early days of IHC. ? Bill is correct in his answer about why it should be stored at the various temps also. ? Pam Marcum ? ----- Original Message ----- From: "WILLIAM DESALVO" To: "Paula Sicurello" , "histonet" , "Microscopy@microscopy.com" Sent: Wednesday, November 20, 2013 9:58:42 AM Subject: RE: [Histonet] -80 degrees rationale Here is my stab at why -80 C. ? Temperatures between 0?C and ?25?C, the enzymatic activity of cells is only slowed but remains active. Below ?40?C physiochemical exchanges are frozen. Cellular morphology is preserved at -80?C. Shelf life of tissue increases as the temperature drops. Once you get below -80?C you will need cryoprotectors and when you use cryoprotectors, temperatures must be below ?130?C and will go as low as ?196?C (liquid nitrogen). ?Antibodies and proteins in solution are stable at ?20?C. William DeSalvo, BS HTL(ASCP) > Date: Wed, 20 Nov 2013 09:25:54 -0500 > From: patpxs@gmail.com > To: histonet@lists.utsouthwestern.edu; Microscopy@microscopy.com > CC: > Subject: [Histonet] -80 degrees rationale > > Hello My Fellow Listers, > > The question of the day is: ?What is the rationale for storing frozen > biopsies at -80 degrees? > > I have seen protocols that range in temperature from -40 to -80 degrees. > > Was -80 selected because that was the lowest freezers could go back in the > day? > > Awaiting your chilly responses! > > Thanks in advance, > > Paula > > -- > Paula Sicurello, HTL (ASCP) > Supervisor, Clinical Electron Microscopy Laboratory > Duke University Health System > Rm.#251M, Duke South, Green Zone > Durham, North Carolina 27710 > P: ? 919.684.2091 > > HIPAA Privacy Notification: This message and any accompanying documents are > covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, > and contain information intended for the specific individual (s) only. This > information is confidential. If you are not the intended recipient or an > agent responsible for delivering it to the intended recipient, you are > hereby notified that you have received this document in error and that any > review, dissemination, copying or the taking of any action based on the > contents of this information is strictly prohibited . If you have received > this communication in error, please notify us immediately by e-mail, and > delete the original message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ????????????????? ???????? ? ???????????????? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wdesalvo.cac <@t> outlook.com Wed Nov 20 10:08:52 2013 From: wdesalvo.cac <@t> outlook.com (WILLIAM DESALVO) Date: Wed Nov 20 10:08:56 2013 Subject: [Histonet] Specimens from surigical units In-Reply-To: References: Message-ID: We use the hospital LIS. All specimens/cases are entered and ordered by Surgery and they are then received by Surgical Pathology. You are able to effectively track w/ pending lists and a full manifest/reconciliation process. William DeSalvo, BS HTL(ASCP) > From: Vickroy.Jim@mhsil.com > To: histonet@lists.utsouthwestern.edu > Date: Wed, 20 Nov 2013 10:03:45 -0600 > Subject: [Histonet] Specimens from surigical units > > > We are a large pathology department and process over 35,000 surgical accessions each year. I am interested in learning how other pathology departments keep track of the specimens that are removed in surgery to make sure they are received in pathology. Obviously all specimens received by pathology are entered into the pathology accessioning system however the question has come up to how do we know that every specimen taken in surgery for pathology is actually received in pathology. A couple of our surgical units utilize volunteer couriers since the surgical units are not near pathology. A couple of other units like special procedures and cardiac surgery have a simple notebook that is a signoff when the specimens are taken to pathology, however from there is no tracking system from the main OR or outpatient surgery. In otherwords a specimen could be removed in the OR that was supposed to be sent to pathology does not arrive in pathology. Obviously this doesn't happen often but we are trying to close the potential for an occurrence. Specimens removed in surgery are logged into the surgical OR report however there is no current way for us to track that everything removed that day that was supposed to go to pathology actually made it to pathology. Any ideas? > > Jim Vickroy > > James Vickroy BS, HT(ASCP) > > Surgical and Autopsy Pathology Technical Supervisor > Memorial Medical Center > 217-788-4046 > > > ________________________________ > This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWatson <@t> gnf.org Wed Nov 20 10:18:26 2013 From: JWatson <@t> gnf.org (James Watson) Date: Wed Nov 20 10:18:31 2013 Subject: [Histonet] -80 freezer question In-Reply-To: References: Message-ID: Paula, I do not remember exactly what study or publication I got this from, but I had to do some research a few years ago because our lab ops department did not want to have our freezer set at -80. I came up with a few reasons: . Some proteins have been shown to break down at -60 but be stable at -80 . DNA and mRNA recovery is better at -80 vs. -60 . Some antibodies are better preserved at -80 o Our Collagen X antibody from Abcam (ab58632) requires -80 storage Diagn Mol Pathol. 1992 Mar;1(1):73-9. Role of the frozen tissue bank in molecular pathology. Naber SP, Smith LL Jr, Wolfe HJ. Source Department of Pathology, Tufts University School of Medicine, New England Medical Center Hospitals, Boston, MA 02111. Abstract The new discipline of molecular pathology requires that high-quality, intact genomic DNA, mRNA, and proteins be available from frozen tissue samples. It is now necessary for pathology laboratories to establish consistent guidelines for the preparation and storage of frozen tissue samples in order to have properly preserved tissues available for diagnostic molecular techniques. Maintaining a frozen tissue bank requires a pathologist to oversee this program and to integrate it into the routine surgical pathology activities. A member of the laboratory technical staff can serve as a tissue bank coordinator and have responsibility for preparation of tissue samples, their systematic storage and retrieval, and routine maintenance of equipment and supplies. Tissue sampling must be done as soon as possible after excision of the specimen and is the responsibility of a qualified pathologist. The samples may be snap frozen without cryoprotection at -78 degrees C or colder for subsequent use in procedures requiring the extraction of genomic DNA, mRNA, or protein. To preserve tissue architecture and cytologic features for immunohistochemistry and in situ hybridization, the tissue should be frozen at -78 degrees C or colder with a cryoprotectant such as OCT. Long-term storage of the frozen tissue is recommended at -140 degrees C or colder in a locked liquid nitrogen freezer, and the record of sample inventory can easily be kept in a computerized database. Tissues sampled and stored under these conditions have been used successfully in a wide variety of molecular techniques. In addition to malignant tumor tissue, samples from benign lesions and normal tissues should be frozen.(ABSTRACT TRUNCATED AT 250 WORDS) PMID: 1342956 [PubMed - indexed for MEDLINE] James Watson HT? ASCP GNF? Genomics Institute of the Novartis Research Foundation Tel??? 858-332-4647 Fax?? 858-812-1915 jwatson@gnf.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello Sent: Tuesday, November 19, 2013 2:23 PM To: HistoNet Subject: [Histonet] -80 freezer question Dear Netters, What is the rationale for storing frozen patient samples at -80? Is there a range that keeps the sample viable but not as cold? Our -80 (which we just inherited) is struggling and I would like to raise the temperature a smidge to ease the stress on the compressor. Thanks! Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 HIPAA Privacy Notification: This message and any accompanying documents are covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, and contain information intended for the specific individual (s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying or the taking of any action based on the contents of this information is strictly prohibited . If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From NMargaryan <@t> luriechildrens.org Wed Nov 20 11:05:18 2013 From: NMargaryan <@t> luriechildrens.org (Margaryan, Naira) Date: Wed Nov 20 11:05:24 2013 Subject: [Histonet] FW: Hemoglobin Message-ID: <34B2EDA118548A4EB35D6E650345BA6431EDCD47@SV-EX11.childrensmemorial.org> Hi histonetters, Do you know how we can stain for hemoglobin in FFPE zebrafish tissue? Thanks in advance, Simone. Ranked nationally in all 10 pediatric specialties by U.S. News & World Report (LCHOC Ver 1.0) This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. If verification is required please request a hard-copy version. (LCHOC VER 1.0) From BDeBrosse-Serra <@t> isisph.com Wed Nov 20 11:06:09 2013 From: BDeBrosse-Serra <@t> isisph.com (Bea DeBrosse-Serra) Date: Wed Nov 20 11:06:19 2013 Subject: [Histonet] Aperio ScanScope XT vs. ScanScope AT Turbo Message-ID: Hi histonetters, I would like to hear from people who made the switch from the original Aperio ScanScope XT to the new ScanScope AT Turbo. We are in the process to possibly change over. My questions are how you like it overall, if you experience any major problems/issues, how much faster it is, response time from vendor if there is an issue/problem etc. Also, if you considered changing to a new vendor, and if so why. Looking forward to hear your input and feedback. Bea Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 From HornHV <@t> archildrens.org Wed Nov 20 11:10:28 2013 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Wed Nov 20 11:10:36 2013 Subject: [Histonet] Specimens from surigical units In-Reply-To: References: Message-ID: <25A4DE08332B19499904459F00AAACB719DA7CAEE3@EVS1.archildrens.org> We have a similar system. When pathology specimens are taken in surgery a notification is generated and sent to our printer. We reconcile the notifications with the requisitions when the specimens are received. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO Sent: Wednesday, November 20, 2013 10:09 AM To: Vickroy, Jim; histonet Subject: RE: [Histonet] Specimens from surigical units We use the hospital LIS. All specimens/cases are entered and ordered by Surgery and they are then received by Surgical Pathology. You are able to effectively track w/ pending lists and a full manifest/reconciliation process. William DeSalvo, BS HTL(ASCP) > From: Vickroy.Jim@mhsil.com > To: histonet@lists.utsouthwestern.edu > Date: Wed, 20 Nov 2013 10:03:45 -0600 > Subject: [Histonet] Specimens from surigical units > > > We are a large pathology department and process over 35,000 surgical accessions each year. I am interested in learning how other pathology departments keep track of the specimens that are removed in surgery to make sure they are received in pathology. Obviously all specimens received by pathology are entered into the pathology accessioning system however the question has come up to how do we know that every specimen taken in surgery for pathology is actually received in pathology. A couple of our surgical units utilize volunteer couriers since the surgical units are not near pathology. A couple of other units like special procedures and cardiac surgery have a simple notebook that is a signoff when the specimens are taken to pathology, however from there is no tracking system from the main OR or outpatient surgery. In otherwords a specimen could be removed in the OR that was supposed to be sent to pathology does not arrive in pathology. Obviously this doesn't happen often but we are trying to close the potential for an occurrence. Specimens removed in surgery are logged into the surgical OR report however there is no current way for us to track that everything removed that day that was supposed to go to pathology actually made it to pathology. Any ideas? > > Jim Vickroy > > James Vickroy BS, HT(ASCP) > > Surgical and Autopsy Pathology Technical Supervisor Memorial Medical > Center > 217-788-4046 > > > ________________________________ > This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** This message is confidential, intended only for the named recipient(s) and may contain information that is privileged, attorney work product, highly confidential, or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender by reply electronic mail, delete this electronic mail from your computer, and destroy any copies in any form immediately. Receipt by anyone other than the named recipient(s) is not a waiver of any attorney-client, work product, or other applicable privilege. From trathborne <@t> somerset-healthcare.com Wed Nov 20 11:18:30 2013 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Wed Nov 20 11:18:57 2013 Subject: [Histonet] Specimens from surigical units In-Reply-To: <25A4DE08332B19499904459F00AAACB719DA7CAEE3@EVS1.archildrens.org> References: <25A4DE08332B19499904459F00AAACB719DA7CAEE3@EVS1.archildrens.org> Message-ID: <3AD061FE740D464FAC7BF6B5CFB75707A8B3EC6C@smcmail02.somerset-healthcare.com> We have a book for each area that sends us specimens. A patient label is placed in the book by the collecting location, with the number of containers collected. The person who places the labels/specimen in the bin will initial the book. When the specimen is received(either picked up or delivered), the person getting the specimen initials after matching the correct number of containers and the patient information. The books are housed in the sender's location, and any discrepancies can be reviewed afterwards. We also have a pending log that prints at the end of the day which we check. If something should have arrived and hasn't, we will call to find out if there was actually a specimen collected. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Wednesday, November 20, 2013 12:10 PM To: 'WILLIAM DESALVO'; Vickroy, Jim; histonet Subject: RE: [Histonet] Specimens from surigical units We have a similar system. When pathology specimens are taken in surgery a notification is generated and sent to our printer. We reconcile the notifications with the requisitions when the specimens are received. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO Sent: Wednesday, November 20, 2013 10:09 AM To: Vickroy, Jim; histonet Subject: RE: [Histonet] Specimens from surigical units We use the hospital LIS. All specimens/cases are entered and ordered by Surgery and they are then received by Surgical Pathology. You are able to effectively track w/ pending lists and a full manifest/reconciliation process. William DeSalvo, BS HTL(ASCP) > From: Vickroy.Jim@mhsil.com > To: histonet@lists.utsouthwestern.edu > Date: Wed, 20 Nov 2013 10:03:45 -0600 > Subject: [Histonet] Specimens from surigical units > > > We are a large pathology department and process over 35,000 surgical accessions each year. I am interested in learning how other pathology departments keep track of the specimens that are removed in surgery to make sure they are received in pathology. Obviously all specimens received by pathology are entered into the pathology accessioning system however the question has come up to how do we know that every specimen taken in surgery for pathology is actually received in pathology. A couple of our surgical units utilize volunteer couriers since the surgical units are not near pathology. A couple of other units like special procedures and cardiac surgery have a simple notebook that is a signoff when the specimens are taken to pathology, however from there is no tracking system from the main OR or outpatient surgery. In otherwords a specimen could be removed in the OR that was supposed to be sent to pathology does not arrive in pathology. Obviously this doesn't happen often but we are trying to close the potential for an occurrence. Specimens removed in surgery are logged into the surgical OR report however there is no current way for us to track that everything removed that day that was supposed to go to pathology actually made it to pathology. Any ideas? > > Jim Vickroy > > James Vickroy BS, HT(ASCP) > > Surgical and Autopsy Pathology Technical Supervisor Memorial Medical > Center > 217-788-4046 > > > ________________________________ > This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** This message is confidential, intended only for the named recipient(s) and may contain information that is privileged, attorney work product, highly confidential, or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender by reply electronic mail, delete this electronic mail from your computer, and destroy any copies in any form immediately. Receipt by anyone other than the named recipient(s) is not a waiver of any attorney-client, work product, or other applicable privilege. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JEllin <@t> yumaregional.org Wed Nov 20 11:18:10 2013 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Wed Nov 20 11:20:18 2013 Subject: [Histonet] Molecular tests Message-ID: I would like two pose a questions out there on histonet. How is everyone handling the ordering of the molecular testing within their facility, through an electronic EMR? How are you keeping track of proper test utilization of this testing? Thanks to all for your help Jesus Ellin Yuma Regional Medical Center ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ From patpxs <@t> gmail.com Wed Nov 20 11:37:01 2013 From: patpxs <@t> gmail.com (Paula Sicurello) Date: Wed Nov 20 11:37:06 2013 Subject: [Histonet] Second Question of the Day: -80 degrees rationale Message-ID: Hello Again, Gayle and Bill have provided the most succinct answers to the question of the day. Second Question: Is there a lower end, say -70 or -75, that provides the same protection as -80? I'm asking all this because I've inherited an old -80 freezer and would like it to last as long as possible. The lab that had this freezer previously had an acceptable range of -70 to -90. Thanks in advance, again, oh wise ones. Paula -- > Paula Sicurello, HTL (ASCP) > Supervisor, Clinical Electron Microscopy Laboratory > Duke University Health System > Rm.#251M, Duke South, Green Zone > Durham, North Carolina 27710 > P: 919.684.2091 > > HIPAA Privacy Notification: This message and any accompanying documents are > covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, > and contain information intended for the specific individual (s) only. This > information is confidential. If you are not the intended recipient or an > agent responsible for delivering it to the intended recipient, you are > hereby notified that you have received this document in error and that any > review, dissemination, copying or the taking of any action based on the > contents of this information is strictly prohibited . If you have received > this communication in error, please notify us immediately by e-mail, and > delete the original message. On Wed, Nov 20, 2013 at 10:58 AM, WILLIAM DESALVO wrote: > Here is my stab at why -80 C. > > Temperatures between 0?C and -25?C, the enzymatic activity of cells is > only slowed but remains active. Below -40?C physiochemical exchanges are > frozen. Cellular morphology is preserved at -80?C. Shelf life of tissue > increases as the temperature drops. Once you get below -80?C you will > need cryoprotectors and when you use cryoprotectors, temperatures must be > below -130?C and will go as low as -196?C (liquid nitrogen). Antibodies > and proteins in solution are stable at -20?C. > > *William DeSalvo,* *BS HTL(ASCP)* > > > Date: Wed, 20 Nov 2013 09:25:54 -0500 > > From: patpxs@gmail.com > > To: histonet@lists.utsouthwestern.edu; Microscopy@microscopy.com > > CC: > > Subject: [Histonet] -80 degrees rationale > > > > > Hello My Fellow Listers, > > > > The question of the day is: What is the rationale for storing frozen > > biopsies at -80 degrees? > > > > I have seen protocols that range in temperature from -40 to -80 degrees. > > > > Was -80 selected because that was the lowest freezers could go back in > the > > day? > > > > Awaiting your chilly responses! > > > > Thanks in advance, > > > > Paula > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 HIPAA Privacy Notification: This message and any accompanying documents are covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, and contain information intended for the specific individual (s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying or the taking of any action based on the contents of this information is strictly prohibited . If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From wdesalvo.cac <@t> outlook.com Wed Nov 20 11:49:15 2013 From: wdesalvo.cac <@t> outlook.com (WILLIAM DESALVO) Date: Wed Nov 20 11:49:21 2013 Subject: [Histonet] RE: Second Question of the Day: -80 degrees rationale In-Reply-To: References: Message-ID: The only way to really know is to validate the temperature for the samples you want to store. The acceptable range must meet the performance of the unit and be in an acceptable range for any manufactureg materials stored in the freezer. All this will be tied up in your SOP. William DeSalvo, BS HTL(ASCP) Date: Wed, 20 Nov 2013 12:37:01 -0500 Subject: Second Question of the Day: -80 degrees rationale From: patpxs@gmail.com To: wdesalvo.cac@outlook.com CC: histonet@lists.utsouthwestern.edu; microscopy@microscopy.com Hello Again, Gayle and Bill have provided the most succinct answers to the question of the day. Second Question: Is there a lower end, say -70 or -75, that provides the same protection as -80? I'm asking all this because I've inherited an old -80 freezer and would like it to last as long as possible. The lab that had this freezer previously had an acceptable range of -70 to -90. Thanks in advance, again, oh wise ones. Paula -- > Paula Sicurello, HTL (ASCP) > Supervisor, Clinical Electron Microscopy Laboratory > Duke University Health System > Rm.#251M, Duke South, Green Zone > Durham, North Carolina 27710 > P: 919.684.2091 > > HIPAA Privacy Notification: This message and any accompanying documents are > covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, > and contain information intended for the specific individual (s) only. This > information is confidential. If you are not the intended recipient or an > agent responsible for delivering it to the intended recipient, you are > hereby notified that you have received this document in error and that any > review, dissemination, copying or the taking of any action based on the > contents of this information is strictly prohibited . If you have received > this communication in error, please notify us immediately by e-mail, and > delete the original message. On Wed, Nov 20, 2013 at 10:58 AM, WILLIAM DESALVO wrote: Here is my stab at why -80 C. Temperatures between 0?C and ?25?C, the enzymatic activity of cells is only slowed but remains active. Below ?40?C physiochemical exchanges are frozen. Cellular morphology is preserved at -80?C. Shelf life of tissue increases as the temperature drops. Once you get below -80?C you will need cryoprotectors and when you use cryoprotectors, temperatures must be below ?130?C and will go as low as ?196?C (liquid nitrogen). Antibodies and proteins in solution are stable at ?20?C. William DeSalvo, BS HTL(ASCP) > Date: Wed, 20 Nov 2013 09:25:54 -0500 > From: patpxs@gmail.com > To: histonet@lists.utsouthwestern.edu; Microscopy@microscopy.com > CC: > Subject: [Histonet] -80 degrees rationale > > Hello My Fellow Listers, > > The question of the day is: What is the rationale for storing frozen > biopsies at -80 degrees? > > I have seen protocols that range in temperature from -40 to -80 degrees. > > Was -80 selected because that was the lowest freezers could go back in the > day? > > Awaiting your chilly responses! > > Thanks in advance, > > Paula > >> _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Paula Sicurello, HTL (ASCP)Supervisor, Clinical Electron Microscopy LaboratoryDuke University Health SystemRm.#251M, Duke South, Green Zone Durham, North Carolina 27710P: 919.684.2091 HIPAA Privacy Notification: This message and any accompanying documents are covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, and contain information intended for the specific individual (s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying or the taking of any action based on the contents of this information is strictly prohibited . If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From jmbrooksokstate <@t> gmail.com Wed Nov 20 12:38:55 2013 From: jmbrooksokstate <@t> gmail.com (Joseph Brooks) Date: Wed Nov 20 12:38:59 2013 Subject: [Histonet] Acid Phosphatase Solution ? Message-ID: Hello all, Is there anyome out there that is performing an Acid Phosphatase stain on Muscle Biopsies? If so is the Veronal Acetate Buffer solution supposed to be cloudy? We are trying to work this stain and the Alkaline Phosphatase stain up for our Neuropathologists and want to make sure the solution is supposed to look the wat it does. Your help is appreciated. Thank you, Matt Brooks Neuropathology Manager From barbara.tibbs <@t> accuratediagnosticlabs.com Wed Nov 20 13:11:58 2013 From: barbara.tibbs <@t> accuratediagnosticlabs.com (Barbara Tibbs) Date: Wed Nov 20 13:12:04 2013 Subject: [Histonet] RE: Leica RM2255 block clamp In-Reply-To: References: Message-ID: <9370a5efd67d405da2560641e44b1f16@BL2PR04MB196.namprd04.prod.outlook.com> Spend the 292 pounds and buy a new clamp. A repaired clamp is never tight enough to hold the cassette as tightly as a new one. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.tibbs@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Finlay Finlay Sent: Tuesday, November 19, 2013 1:29 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica RM2255 block clamp Hello Hoping someone might be able to help with this microtome issue. I have a problem with the block clamp on one of my Leica RM2255's. It has recently loosened up and it is now possible to move the clamped cassette from side-to-side when the clamp is shut. When I turn the bolt on the underside of the clamp it neither tightens up nor loosens off so there does not seem to be a way of taking the clamp apart any further. Leica have suggested that I buy a new block clamp (?292) but it just feels like something that could be easily fixed, perhaps with a new spring? Has anyone had experience with this problem? Thank you Finlay Finlay Histology Technician Forensic Medicine and Science School of Medicine College of Medical, Veterinary and Life Sciences University of Glasgow Joseph Black building Direct Line: +44 (0) 141 3303443 E-mail: finlay.finlay@glasgow.ac.uk The University of Glasgow Charity Number SC004401 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Pat.Bell <@t> ucdenver.edu Wed Nov 20 13:35:40 2013 From: Pat.Bell <@t> ucdenver.edu (Bell, Pat) Date: Wed Nov 20 13:36:16 2013 Subject: [Histonet] PDL-1/PD-1 Message-ID: <64DB27005E2FD3439E88502D7A5C912101024B94AE27@CORTEZ.ucdenver.pvt> Hello to everyone! Has anyone used PDL-1/PD-1 on FFPE tissue? Could I please get a copy of your protocol and where you purchased the antibody? Thank you so much. Pat Pat Bell HT(ASCP) Sr. PRA University of Colorado, Denver Medical Oncology; MS 8117 12801 E 17th Ave Aurora, Colorado 80045 303-724-6077 pat.bell@ucdenver.edu From ckowalcyk <@t> gmail.com Wed Nov 20 20:22:47 2013 From: ckowalcyk <@t> gmail.com (Carol Kowalcyk) Date: Wed Nov 20 20:23:11 2013 Subject: [Histonet] what are the Clia regulations for an Ht to gross??? Message-ID: <351EA433-CFDA-4D7D-B169-60C4A1483C0D@gmail.com> From Finlay.Finlay <@t> glasgow.ac.uk Thu Nov 21 08:55:59 2013 From: Finlay.Finlay <@t> glasgow.ac.uk (Finlay Finlay) Date: Thu Nov 21 08:56:06 2013 Subject: [Histonet] RE: Leica RM2255 block clamp In-Reply-To: <9370a5efd67d405da2560641e44b1f16@BL2PR04MB196.namprd04.prod.outlook.com> References: <9370a5efd67d405da2560641e44b1f16@BL2PR04MB196.namprd04.prod.outlook.com> Message-ID: Thank you to everyone who took the time to advise me about the block clamp. Looks like I'm going to buy a new clamp and also get out University engineering guys to have a tinker with the old one. Finlay ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Finlay Finlay Sent: Tuesday, November 19, 2013 1:29 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica RM2255 block clamp Hello Hoping someone might be able to help with this microtome issue. I have a problem with the block clamp on one of my Leica RM2255's. It has recently loosened up and it is now possible to move the clamped cassette from side-to-side when the clamp is shut. When I turn the bolt on the underside of the clamp it neither tightens up nor loosens off so there does not seem to be a way of taking the clamp apart any further. Leica have suggested that I buy a new block clamp (?292) but it just feels like something that could be easily fixed, perhaps with a new spring? Has anyone had experience with this problem? Thank you Finlay Finlay Histology Technician Forensic Medicine and Science School of Medicine College of Medical, Veterinary and Life Sciences University of Glasgow Joseph Black building Direct Line: +44 (0) 141 3303443 E-mail: finlay.finlay@glasgow.ac.uk The University of Glasgow Charity Number SC004401 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amber.mckenzie <@t> gastrodocs.net Thu Nov 21 11:24:08 2013 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Thu Nov 21 11:24:20 2013 Subject: [Histonet] Esophageal Brushings In-Reply-To: <5bae6ce3beab4ae38e21e5622fae4400@BLUPR07MB178.namprd07.prod.outlook.com> References: <5A33C952BB67F4468AF1F36D739212BCBD6EA704@JERRY.Gia.com> <95F6E043-FD0B-46B1-9A77-A20B7A72F1EF@yahoo.com> <5bae6ce3beab4ae38e21e5622fae4400@BLUPR07MB178.namprd07.prod.outlook.com> Message-ID: <5A33C952BB67F4468AF1F36D739212BCDD11E95A@JERRY.Gia.com> Do any of you do Esophageal Brushings in your lab? If so, what do you use to stain the slides...just coplin jars side by side dipping the slides in or some type of stainer? Who do you order your supplies from? There were a couple of stains I didn't recognize in the protocol we are supposed to use. This is all new to me. Thanks! From jclark <@t> pcnm.com Thu Nov 21 12:09:34 2013 From: jclark <@t> pcnm.com (Joanne Clark) Date: Thu Nov 21 12:09:40 2013 Subject: [Histonet] Clia grossing regulations In-Reply-To: <20131121180249.6CDB563EC1B@mx10.myoutlookonline.com> References: <20131121180249.6CDB563EC1B@mx10.myoutlookonline.com> Message-ID: <0494A7D4E8CC254EA2FB81464982E378B4AD92CD@S10MAILD001N4.SH10.lan> Carol, here they are: **REVISED** 07/11/2011 ANP.11610 Gross Examination Qualifications Phase II If individuals other than a pathologist or pathology resident assist in gross examinations, such individuals qualify as high complexity testing personnel under CLIA regulations. NOTE: The laboratory director may delegate the dissection of specimens to non-pathologist individuals; these individuals must be qualified as high complexity testing personnel under CLIA regulations. The minimum training/experience required of such personnel is: 1. An earned associate degree in a laboratory science or medical laboratory technology, obtained from an accredited institution, OR 2. Education/training equivalent to the above that includes at least 60 semester hours or equivalent from an accredited institution. This education must include 24 semester hours of medical laboratory technology courses, OR 24 semester hours of science courses that includes 6 semester hours of chemistry, 6 semester hours of biology, and 12 semester hours of chemistry, biology or medical laboratory technology in any combination. In addition, the individual must have laboratory training including either completion of a clinical laboratory training program approved or accredited by the ABHES, NAACLA, or other organization approved by HHS (note that this training may be included in the 60 semester hours listed above), OR at least 3 months documented laboratory training in each specialty in which the individual performs high complexity testing. It is the responsibility of the laboratory director to determine whether an individual's education, training and experience satisfies the requirements of this checklist requirement. This checklist requirement applies only to laboratories subject to US regulations. Joanne Clark, HT Director of Histology Pathology Consultants of New Mexico -------------------------------------------------------------------------------------- Message: 4 Date: Wed, 20 Nov 2013 21:22:47 -0500 From: Carol Kowalcyk Subject: [Histonet] what are the Clia regulations for an Ht to gross??? To: Histonet@lists.utsouthwestern.edu Message-ID: <351EA433-CFDA-4D7D-B169-60C4A1483C0D@gmail.com> Content-Type: text/plain * Disclaimer: This electronic message may contain information that is proprietary, confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From kmilne <@t> bccancer.bc.ca Thu Nov 21 12:33:23 2013 From: kmilne <@t> bccancer.bc.ca (Milne, Katy) Date: Thu Nov 21 12:33:28 2013 Subject: [Histonet] PDL-1/PD-1 In-Reply-To: <11d5d826-8412-49b3-bd5c-bc9a2506f44f@SRVEXHT01.phsabc.ehcnet.ca> References: <11d5d826-8412-49b3-bd5c-bc9a2506f44f@SRVEXHT01.phsabc.ehcnet.ca> Message-ID: <3FEFF18FF4E1914A9AB7D8498591BE8602BAE772E21A@VEXCCR02.phsabc.ehcnet.ca> Hi Pat, We've been working with PD-1 and PD-L1. For PD-1, we use the MRQ-22 clone from Cell Marque, it has worked really well for us. We use it at 1/250 for 30min with Biocare reagents (Mach2 Mouse polymers), I've been doing mostly multicolour IHC with it. For PD-L1 we use a mouse MAb from MBL cat # D230-3 clone 27A2. It was the same Ab used in this paper - PMID 17360651 I've tried a few different concentrations with this one so it's best to see what it does in your hands. In our hands, most of our samples come up positive (ovarian cancer) but it's not as striking as it would be for membrane or nuclear markers. If you have any other questions, please let me know. Katy ------------------------------ Message: 3 Date: Wed, 20 Nov 2013 12:35:40 -0700 From: "Bell, Pat" Subject: [Histonet] PDL-1/PD-1 To: "histonet@lists.utsouthwestern.edu" Message-ID: <64DB27005E2FD3439E88502D7A5C912101024B94AE27@CORTEZ.ucdenver.pvt> Content-Type: text/plain; charset="us-ascii" Hello to everyone! Has anyone used PDL-1/PD-1 on FFPE tissue? Could I please get a copy of your protocol and where you purchased the antibody? Thank you so much. Pat Pat Bell HT(ASCP) Sr. PRA University of Colorado, Denver Medical Oncology; MS 8117 12801 E 17th Ave Aurora, Colorado 80045 303-724-6077 pat.bell@ucdenver.edu From mfisher <@t> pmhd.org Thu Nov 21 12:36:39 2013 From: mfisher <@t> pmhd.org (Maura Fisher) Date: Thu Nov 21 12:36:45 2013 Subject: [Histonet] What can go down the drain in California? Message-ID: <1DDC0ABD164CBD4595BBD4AFEE2890C2A4FE1E@PMH-EXMB2.pmhd.local.pmhd.org> I am a traveling tech from the East Coast and not familiar with chemical waste disposal laws in California. If there is anyone on here from a California lab can you help me? How do you dispose of chemicals like alcohol? What about special stain waste? What can go down the drain? Help!! Thanks in advance!!! :) From SHargrove <@t> unitedregional.org Thu Nov 21 14:12:04 2013 From: SHargrove <@t> unitedregional.org (Susie Hargrove) Date: Thu Nov 21 14:12:08 2013 Subject: [Histonet] Cleaning stainless steel base molds Message-ID: Hello, I was wondering if labs are still cleaning their base molds? We did years ago, like 15 years ago. Our blocks always pop right out and rarely do we have anything left in bottom. If that happens we do clean that one, but that is usually due to the poorly processed tissue that was in it. ________________________________ Susie Hargrove HT (ASCP) Histology Technical Specialist United Regional Health Care Wichita Falls, Texas 76301 ________________________________ From DKBoyd <@t> chs.net Thu Nov 21 14:37:35 2013 From: DKBoyd <@t> chs.net (Boyd, Debbie M) Date: Thu Nov 21 14:37:45 2013 Subject: [Histonet] RE: Esophageal Brushings In-Reply-To: <5A33C952BB67F4468AF1F36D739212BCDD11E95A@JERRY.Gia.com> References: <5A33C952BB67F4468AF1F36D739212BCBD6EA704@JERRY.Gia.com> <95F6E043-FD0B-46B1-9A77-A20B7A72F1EF@yahoo.com> <5bae6ce3beab4ae38e21e5622fae4400@BLUPR07MB178.namprd07.prod.outlook.com> <5A33C952BB67F4468AF1F36D739212BCDD11E95A@JERRY.Gia.com> Message-ID: <7EAFE982E328304DA6CE2B677BB76246795022DE@TN001WEXMBX12.US.chs.net> We make 2 cytospins and stain them with the Pap stain. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Thursday, November 21, 2013 12:24 PM Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] Esophageal Brushings Do any of you do Esophageal Brushings in your lab? If so, what do you use to stain the slides...just coplin jars side by side dipping the slides in or some type of stainer? Who do you order your supplies from? There were a couple of stains I didn't recognize in the protocol we are supposed to use. This is all new to me. Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From rjbuesa <@t> yahoo.com Thu Nov 21 14:53:28 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Nov 21 14:53:37 2013 Subject: [Histonet] Cleaning stainless steel base molds In-Reply-To: References: Message-ID: <1385067208.43595.YahooMailNeo@web163106.mail.bf1.yahoo.com> I put them along with the cassettes stainless steel covers and the embedding forceps?in the VIP cleaning cycle Ren? J. ________________________________ From: Susie Hargrove To: "histonet@lists.utsouthwestern.edu" Sent: Thursday, November 21, 2013 3:12 PM Subject: [Histonet] Cleaning stainless steel base molds Hello, I was wondering if labs are still cleaning their base molds? We did years ago, like 15 years ago. Our blocks always? pop right out and rarely do we have anything left in bottom. If that happens we do clean that one, but that is usually due to the poorly processed tissue that was in it. ________________________________ Susie Hargrove? HT (ASCP) Histology Technical Specialist United Regional Health Care Wichita Falls, Texas 76301 ________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joewalker <@t> rrmc.org Thu Nov 21 15:37:30 2013 From: joewalker <@t> rrmc.org (Joe W. Walker, Jr.) Date: Thu Nov 21 15:37:36 2013 Subject: [Histonet] RE: Esophageal Brushings In-Reply-To: <7EAFE982E328304DA6CE2B677BB76246795022DE@TN001WEXMBX12.US.chs.net> References: <5A33C952BB67F4468AF1F36D739212BCBD6EA704@JERRY.Gia.com> <95F6E043-FD0B-46B1-9A77-A20B7A72F1EF@yahoo.com> <5bae6ce3beab4ae38e21e5622fae4400@BLUPR07MB178.namprd07.prod.outlook.com> <5A33C952BB67F4468AF1F36D739212BCDD11E95A@JERRY.Gia.com> <7EAFE982E328304DA6CE2B677BB76246795022DE@TN001WEXMBX12.US.chs.net> Message-ID: <3C2378778400AD448ADA6FD6BDB7CCCC181450BE@RRMBX03.rrmc.local> Wow are the brushes sent to you? In a collection medium like cytorich red or saline? Are slides prepared with the brush and fixed or air dried? Cytospins could work and a Pap stain would be preferred on a fixed slide but perhaps your pathologist want a Romanowsky type stained slide too since air dried slides can show things that a fixed slide will not. Joe W. Walker, Jr. MS, SCT(ASCP)CM Manager of Anatomical Pathology, Microbiology and Reference Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P: 802.747.1790 F: 802.747.6525 Email joewalker@rrmc.org www.rrmc.org Our Vision: To be the Best Community Healthcare System in New England Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet Recognition? and the Governor's Award for Performance Excellence -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Boyd, Debbie M Sent: Thursday, November 21, 2013 3:38 PM To: Amber McKenzie Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Esophageal Brushings We make 2 cytospins and stain them with the Pap stain. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Thursday, November 21, 2013 12:24 PM Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] Esophageal Brushings Do any of you do Esophageal Brushings in your lab? If so, what do you use to stain the slides...just coplin jars side by side dipping the slides in or some type of stainer? Who do you order your supplies from? There were a couple of stains I didn't recognize in the protocol we are supposed to use. This is all new to me. Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. Thank You From amber.mckenzie <@t> gastrodocs.net Thu Nov 21 15:50:17 2013 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Thu Nov 21 15:50:21 2013 Subject: [Histonet] RE: Esophageal Brushings In-Reply-To: <3C2378778400AD448ADA6FD6BDB7CCCC181450BE@RRMBX03.rrmc.local> References: <5A33C952BB67F4468AF1F36D739212BCBD6EA704@JERRY.Gia.com> <95F6E043-FD0B-46B1-9A77-A20B7A72F1EF@yahoo.com> <5bae6ce3beab4ae38e21e5622fae4400@BLUPR07MB178.namprd07.prod.outlook.com> <5A33C952BB67F4468AF1F36D739212BCDD11E95A@JERRY.Gia.com> <7EAFE982E328304DA6CE2B677BB76246795022DE@TN001WEXMBX12.US.chs.net> <3C2378778400AD448ADA6FD6BDB7CCCC181450BE@RRMBX03.rrmc.local> Message-ID: <5A33C952BB67F4468AF1F36D739212BCDD11EB14@JERRY.Gia.com> We haven't started this procedure yet. I'm just trying to get an idea of what to expect and what to order to be prepared. -----Original Message----- From: Joe W. Walker, Jr. [mailto:joewalker@rrmc.org] Sent: Thursday, November 21, 2013 3:38 PM To: Boyd, Debbie M; Amber McKenzie Cc: histonet@lists.utsouthwestern.edu Subject: RE: Esophageal Brushings Wow are the brushes sent to you? In a collection medium like cytorich red or saline? Are slides prepared with the brush and fixed or air dried? Cytospins could work and a Pap stain would be preferred on a fixed slide but perhaps your pathologist want a Romanowsky type stained slide too since air dried slides can show things that a fixed slide will not. Joe W. Walker, Jr. MS, SCT(ASCP)CM Manager of Anatomical Pathology, Microbiology and Reference Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P: 802.747.1790 F: 802.747.6525 Email joewalker@rrmc.org www.rrmc.org Our Vision: To be the Best Community Healthcare System in New England Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet Recognition(r) and the Governor's Award for Performance Excellence -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Boyd, Debbie M Sent: Thursday, November 21, 2013 3:38 PM To: Amber McKenzie Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Esophageal Brushings We make 2 cytospins and stain them with the Pap stain. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Thursday, November 21, 2013 12:24 PM Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] Esophageal Brushings Do any of you do Esophageal Brushings in your lab? If so, what do you use to stain the slides...just coplin jars side by side dipping the slides in or some type of stainer? Who do you order your supplies from? There were a couple of stains I didn't recognize in the protocol we are supposed to use. This is all new to me. Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. Thank You From ggracie <@t> stvincents.com.au Thu Nov 21 17:02:33 2013 From: ggracie <@t> stvincents.com.au (Gary Gracie) Date: Thu Nov 21 17:03:07 2013 Subject: [Histonet] PD-1 Message-ID: <1443398b97c24897b84d6bc8b3a1db26@SVMHSEXCH02.svmhs.stvincents.com.au> Dear Pat We use Cell Marque PD-1 cat# 315M-96 Contact me if you would like to know our protocol for Ventana stainers Regards Gary Gracie Senior Technical Officer IHC Laboratory Anatomical Pathology St Vincents Hospital Sydney Australia Hello to everyone! Has anyone used PDL-1/PD-1 on FFPE tissue? Could I please get a copy of your protocol and where you purchased the antibody? Thank you so much. Pat Pat Bell HT(ASCP) Sr. PRA University of Colorado, Denver Medical Oncology; MS 8117 12801 E 17th Ave Aurora, Colorado 80045 303-724-6077 pat.bell@ucdenver.edu ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the system manager. This footnote also confirms that this email message has been virus scanned and although no viruses were detected by the system, St Vincents & Mater Health Sydney accepts no liability for any consequential damage resulting from email containing any computer viruses. ********************************************************************** From lori.garcia <@t> medtronic.com Thu Nov 21 18:49:22 2013 From: lori.garcia <@t> medtronic.com (Garcia, Lori, M.Sc.) Date: Thu Nov 21 18:49:32 2013 Subject: [Histonet] large disposable embedding molds Message-ID: <8214022B32A9DE4985A37EA7AE814DA713DF73@MSPM1BMSGM41.ent.core.medtronic.com> Hi Histonetters, A couple of years ago I ordered: VWR Mega-Plus cassette 53x75x19 pk/100, catalog # 18000-288 I don't need the cassettes, but I desperately need the disposable molds; stainless steel won't work. These are not being sold anymore, and I can't find an alternate anywhere. The size of the mold is ~64 x 48 x 15 mm. If anyone has some of these in their lab and would be willing to part with them, I will be happy to purchase from you. Thanks in advance, Lori [CONFIDENTIALITY AND PRIVACY NOTICE] Information transmitted by this email is proprietary to Medtronic and is intended for use only by the individual or entity to which it is addressed, and may contain information that is private, privileged, confidential or exempt from disclosure under applicable law. If you are not the intended recipient or it appears that this mail has been forwarded to you without proper authority, you are notified that any use or dissemination of this information in any manner is strictly prohibited. In such cases, please delete this mail from your records. To view this notice in other languages you can either select the following link or manually copy and paste the link into the address bar of a web browser: http://emaildisclaimer.medtronic.com From benjamin <@t> histologistics.com Fri Nov 22 06:21:13 2013 From: benjamin <@t> histologistics.com (Benjamin) Date: Fri Nov 22 06:21:24 2013 Subject: [Histonet] Cleaning stainless steel base molds In-Reply-To: <1385067208.43595.YahooMailNeo@web163106.mail.bf1.yahoo.com> References: <1385067208.43595.YahooMailNeo@web163106.mail.bf1.yahoo.com> Message-ID: It wont hurt to clean them. I think the dishwasher works good too. It works good on the waterbaths and forcepts also. Sent from my iPhone > On Nov 21, 2013, at 3:53 PM, Rene J Buesa wrote: > > I put them along with the cassettes stainless steel covers and the embedding forceps in the VIP cleaning cycle > Ren? J. > > > ________________________________ > From: Susie Hargrove > To: "histonet@lists.utsouthwestern.edu" > Sent: Thursday, November 21, 2013 3:12 PM > Subject: [Histonet] Cleaning stainless steel base molds > > > Hello, I was wondering if labs are still cleaning their base molds? We did years ago, like 15 years ago. Our blocks always pop right out and rarely do we have anything left in bottom. If that happens we do clean that one, but that is usually due to the poorly processed tissue that was in it. > > ________________________________ > > > > Susie Hargrove HT (ASCP) > > Histology Technical Specialist > > United Regional Health Care > > Wichita Falls, Texas 76301 > > > > > > > > > > > > > > > > > > ________________________________ > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DKBoyd <@t> chs.net Fri Nov 22 06:52:14 2013 From: DKBoyd <@t> chs.net (Boyd, Debbie M) Date: Fri Nov 22 06:52:25 2013 Subject: [Histonet] RE: Esophageal Brushings In-Reply-To: <3C2378778400AD448ADA6FD6BDB7CCCC181450BE@RRMBX03.rrmc.local> References: <5A33C952BB67F4468AF1F36D739212BCBD6EA704@JERRY.Gia.com> <95F6E043-FD0B-46B1-9A77-A20B7A72F1EF@yahoo.com> <5bae6ce3beab4ae38e21e5622fae4400@BLUPR07MB178.namprd07.prod.outlook.com> <5A33C952BB67F4468AF1F36D739212BCDD11E95A@JERRY.Gia.com> <7EAFE982E328304DA6CE2B677BB76246795022DE@TN001WEXMBX12.US.chs.net> <3C2378778400AD448ADA6FD6BDB7CCCC181450BE@RRMBX03.rrmc.local> Message-ID: <7EAFE982E328304DA6CE2B677BB7624679502376@TN001WEXMBX12.US.chs.net> We receive the brushes in saline. We vortex the brush (in the saline) to dislodge any cells attached to it. Spin down the specimen, pipette off the supernant, and make 2 cytospins from the button. You can than perform your stain. There isn't any fixative at this point. For cyptologic evaluation you need to fix them immediately in 95% EtOH . For IHC stains air dry them. -----Original Message----- From: Joe W. Walker, Jr. [mailto:joewalker@rrmc.org] Sent: Thursday, November 21, 2013 4:38 PM To: Boyd, Debbie M; Amber McKenzie Cc: histonet@lists.utsouthwestern.edu Subject: RE: Esophageal Brushings Wow are the brushes sent to you? In a collection medium like cytorich red or saline? Are slides prepared with the brush and fixed or air dried? Cytospins could work and a Pap stain would be preferred on a fixed slide but perhaps your pathologist want a Romanowsky type stained slide too since air dried slides can show things that a fixed slide will not. Joe W. Walker, Jr. MS, SCT(ASCP)CM Manager of Anatomical Pathology, Microbiology and Reference Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P: 802.747.1790 F: 802.747.6525 Email joewalker@rrmc.org www.rrmc.org Our Vision: To be the Best Community Healthcare System in New England Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet Recognition(r) and the Governor's Award for Performance Excellence -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Boyd, Debbie M Sent: Thursday, November 21, 2013 3:38 PM To: Amber McKenzie Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Esophageal Brushings We make 2 cytospins and stain them with the Pap stain. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Thursday, November 21, 2013 12:24 PM Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] Esophageal Brushings Do any of you do Esophageal Brushings in your lab? If so, what do you use to stain the slides...just coplin jars side by side dipping the slides in or some type of stainer? Who do you order your supplies from? There were a couple of stains I didn't recognize in the protocol we are supposed to use. This is all new to me. Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. Thank You From joewalker <@t> rrmc.org Fri Nov 22 07:21:01 2013 From: joewalker <@t> rrmc.org (Joe W. Walker, Jr.) Date: Fri Nov 22 07:21:23 2013 Subject: [Histonet] RE: Esophageal Brushings In-Reply-To: <7EAFE982E328304DA6CE2B677BB7624679502376@TN001WEXMBX12.US.chs.net> References: <5A33C952BB67F4468AF1F36D739212BCBD6EA704@JERRY.Gia.com> <95F6E043-FD0B-46B1-9A77-A20B7A72F1EF@yahoo.com> <5bae6ce3beab4ae38e21e5622fae4400@BLUPR07MB178.namprd07.prod.outlook.com> <5A33C952BB67F4468AF1F36D739212BCDD11E95A@JERRY.Gia.com> <7EAFE982E328304DA6CE2B677BB76246795022DE@TN001WEXMBX12.US.chs.net> <3C2378778400AD448ADA6FD6BDB7CCCC181450BE@RRMBX03.rrmc.local> <7EAFE982E328304DA6CE2B677BB7624679502376@TN001WEXMBX12.US.chs.net> Message-ID: <3C2378778400AD448ADA6FD6BDB7CCCC18145EF5@RRMBX03.rrmc.local> Debbie's method would work very well but in our experience with cytospins, if the fixation doesn't occur prior to the cells being applied the slide but after, you can end up with air drying artifact on the slides you have destined for the Pap stain. If your pathologist want only Pap stained slides, then you could add a small amount cytospin collection fluid to the button, then add a few drops to your cytospin apparatus. There is one caution if you do add this fluid. The fluid contains a carbomer that must be dissolved by soaking in 95% ETOH prior to performing the Pap stain. The carbomer helps protect the cells from air drying and it also helps the cells adhere to the slide. In our method, we follow Debbie's but we add a couple of drops of unfixed, resuspended cells from the button to 1 apparatus and then add fixative to the button for adding to the second apparatus. This way we get a slide for the Pap stain and then a slide for a Romanowsky (we use May-Grunwald Geimsa) stain. As Debbie has pointed out, the unfixed slide can also be used for immunostains but we rarely perform them on GI brushing specimens at our institution. This is how our pathologist perform to review these cases when we perform cytospins on cases. With that said, we now primarily utilize the ThinPrep system for most of our Non-GYN specimens. This would probably be an expensive option if you are only collecting esophageal brushings. Joe W. Walker, Jr. MS, SCT(ASCP)CM Manager of Anatomical Pathology, Microbiology and Reference Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P: 802.747.1790 F: 802.747.6525 Email joewalker@rrmc.org www.rrmc.org Our Vision: To be the Best Community Healthcare System in New England Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet Recognition? and the Governor's Award for Performance Excellence -----Original Message----- From: Boyd, Debbie M [mailto:DKBoyd@chs.net] Sent: Friday, November 22, 2013 7:52 AM To: Joe W. Walker, Jr.; Amber McKenzie Cc: histonet@lists.utsouthwestern.edu Subject: RE: Esophageal Brushings We receive the brushes in saline. We vortex the brush (in the saline) to dislodge any cells attached to it. Spin down the specimen, pipette off the supernant, and make 2 cytospins from the button. You can than perform your stain. There isn't any fixative at this point. For cyptologic evaluation you need to fix them immediately in 95% EtOH . For IHC stains air dry them. -----Original Message----- From: Joe W. Walker, Jr. [mailto:joewalker@rrmc.org] Sent: Thursday, November 21, 2013 4:38 PM To: Boyd, Debbie M; Amber McKenzie Cc: histonet@lists.utsouthwestern.edu Subject: RE: Esophageal Brushings Wow are the brushes sent to you? In a collection medium like cytorich red or saline? Are slides prepared with the brush and fixed or air dried? Cytospins could work and a Pap stain would be preferred on a fixed slide but perhaps your pathologist want a Romanowsky type stained slide too since air dried slides can show things that a fixed slide will not. Joe W. Walker, Jr. MS, SCT(ASCP)CM Manager of Anatomical Pathology, Microbiology and Reference Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P: 802.747.1790 F: 802.747.6525 Email joewalker@rrmc.org www.rrmc.org Our Vision: To be the Best Community Healthcare System in New England Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet Recognition(r) and the Governor's Award for Performance Excellence -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Boyd, Debbie M Sent: Thursday, November 21, 2013 3:38 PM To: Amber McKenzie Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Esophageal Brushings We make 2 cytospins and stain them with the Pap stain. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Thursday, November 21, 2013 12:24 PM Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] Esophageal Brushings Do any of you do Esophageal Brushings in your lab? If so, what do you use to stain the slides...just coplin jars side by side dipping the slides in or some type of stainer? Who do you order your supplies from? There were a couple of stains I didn't recognize in the protocol we are supposed to use. This is all new to me. Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. Thank You This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. Thank You From joelleweaver <@t> hotmail.com Fri Nov 22 07:38:38 2013 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Fri Nov 22 07:38:46 2013 Subject: [Histonet] Clia grossing regulations In-Reply-To: <0494A7D4E8CC254EA2FB81464982E378B4AD92CD@S10MAILD001N4.SH10.lan> References: <20131121180249.6CDB563EC1B@mx10.myoutlookonline.com>, <0494A7D4E8CC254EA2FB81464982E378B4AD92CD@S10MAILD001N4.SH10.lan> Message-ID: The minimum training/experience required of such personnel is: 1. An earned associate degree in a laboratory science or medical laboratory technology, obtained from an accredited institution, OR 2. Education/training equivalent to the above that includes at least 60 semester hours or equivalent from an accredited institution. This education must include 24 semester hours of medical laboratory technology courses, OR 24 semester hours of science courses that includes 6 semester hours of chemistry, 6 semester hours of biology, and 12 semester hours of chemistry, biology or medical laboratory technology in any combination. In addition, the individual must have laboratory training including either completion of a clinical laboratory training program approved or accredited by the ABHES, the CAHEA, or other organization approved by HHS (note that this training may be included in the 60 semester hours listed above), OR at least 3 months documented laboratory training in each specialty in which the individual performs high complexity testing. In addition, the CLIA-88 regulations include exceptions for grandfathered individuals; these regulations (42CFR493.1489 and 1491) may be found at http://www.phppo.cdc.gov/clia/regs/subpart_m.aspx#493.1487 Joelle Weaver MAOM, HTL (ASCP) QIHC > From: jclark@pcnm.com > To: histonet@lists.utsouthwestern.edu > Date: Thu, 21 Nov 2013 18:09:34 +0000 > Subject: [Histonet] Clia grossing regulations > > Carol, here they are: > > **REVISED** 07/11/2011 > ANP.11610 Gross Examination Qualifications Phase II > If individuals other than a pathologist or pathology resident assist in gross examinations, > such individuals qualify as high complexity testing personnel under CLIA regulations. > NOTE: The laboratory director may delegate the dissection of specimens to non-pathologist > individuals; these individuals must be qualified as high complexity testing personnel under CLIA > regulations. The minimum training/experience required of such personnel is: > 1. An earned associate degree in a laboratory science or medical laboratory technology, > obtained from an accredited institution, OR > 2. Education/training equivalent to the above that includes at least 60 semester hours or > equivalent from an accredited institution. This education must include 24 semester > hours of medical laboratory technology courses, OR 24 semester hours of science > courses that includes 6 semester hours of chemistry, 6 semester hours of biology, and > 12 semester hours of chemistry, biology or medical laboratory technology in any > combination. In addition, the individual must have laboratory training including either > completion of a clinical laboratory training program approved or accredited by the > ABHES, NAACLA, or other organization approved by HHS (note that this training may > be included in the 60 semester hours listed above), OR at least 3 months documented > laboratory training in each specialty in which the individual performs high complexity > testing. > It is the responsibility of the laboratory director to determine whether an individual's education, > training and experience satisfies the requirements of this checklist requirement. > This checklist requirement applies only to laboratories subject to US regulations. > > Joanne Clark, HT > Director of Histology > Pathology Consultants of New Mexico > -------------------------------------------------------------------------------------- > > Message: 4 > Date: Wed, 20 Nov 2013 21:22:47 -0500 > From: Carol Kowalcyk > Subject: [Histonet] what are the Clia regulations for an Ht to > gross??? > To: Histonet@lists.utsouthwestern.edu > Message-ID: <351EA433-CFDA-4D7D-B169-60C4A1483C0D@gmail.com> > Content-Type: text/plain > > > > > * > > > > Disclaimer: This electronic message may contain information that is proprietary, > confidential, or legally privileged or protected. It is intended only for the use > of the individual(s) and entity named in the message. If you are not an intended > recipient of this message, please notify the sender immediately and delete the > material from your computer. Do not deliver, distribute or copy this message and > do not disclose its contents or take any action in reliance on the information it > contains. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ahc53 <@t> georgetown.edu Fri Nov 22 07:40:49 2013 From: ahc53 <@t> georgetown.edu (Anna Coffey) Date: Fri Nov 22 07:40:54 2013 Subject: [Histonet] ASCP HTL Certification Exam Message-ID: Hello Histonetters, I am beginning to study for my HTL certification exam and I am a bit overwhelmed by all the available study aids. Since there are so many (and all seem to be quite pricey), I thought some of you who have been through this before might be able to provide some guidance on the best tools you used to prepare. I would really appreciate any advice you can give on exam prep! Thanks, Anna -- Anna Coffey Senior Histology Technician Department of Oncology Histopathology and Tissue Shared Resource LR-10 Pre-Clinical Sciences Building Lombardi Comprehensive Cancer Center Georgetown University 202-687-7890 ahc53@georgetown.edu From jcox90 <@t> yahoo.com Fri Nov 22 07:48:23 2013 From: jcox90 <@t> yahoo.com (Jill Cox) Date: Fri Nov 22 07:48:27 2013 Subject: [Histonet] Nails falling off slides Message-ID: <1385128103.33810.YahooMailBasic@web161602.mail.bf1.yahoo.com> Hi All, I know this has been mentioned a few times but need some help with nails falling off charged slides for H&E and PAS stains. I use Statlab nail prep softener and also use the good charged slides. I heat them 2 times for 25 minutes each. Is there an adhesive I can put on slide? Albumin? Any help would be much appreciated, thanks in advance.. Jill Cox HT, (ASCP) Histology Manager Arrowhead Dermatology 7747 W Deer Valley Rd Ste 250 Peoria AZ 85382 480-257-7222 From relia1 <@t> earthlink.net Fri Nov 22 10:56:55 2013 From: relia1 <@t> earthlink.net (Pam Barker) Date: Fri Nov 22 10:57:04 2013 Subject: [Histonet] OT/: TGIF Friday Funny As heard on Facebook. Message-ID: <00dc01cee7a3$da229cb0$8e67d610$@earthlink.net> "It's Thanksgiving time don't forget to set your scales back 10 lbs" Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From joelleweaver <@t> hotmail.com Fri Nov 22 12:47:56 2013 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Fri Nov 22 12:48:00 2013 Subject: [Histonet] Clia grossing regulations In-Reply-To: <3D4A471B82E7A44C87F6839732320D9F03AC33@VSPDMS-ITEXMB02.DMS.COM> References: <20131121180249.6CDB563EC1B@mx10.myoutlookonline.com>, , <0494A7D4E8CC254EA2FB81464982E378B4AD92CD@S10MAILD001N4.SH10.lan>, , <3D4A471B82E7A44C87F6839732320D9F03AC33@VSPDMS-ITEXMB02.DMS.COM> Message-ID: There are those who can perform under the grandfather clauses in CLIA still working. What I put up earlier was straight from the CAP AP checklist for grossing for non-pathologist; I interpret the CLIA statement to mean at least an associates or what they say is equivalent in college credits, plus the training-either as a completion of a formal program OR the 3 months documented laboratory training in each specialty in which the individual performs high complexity testing. Not the college credits as the "OR" but the formal program versus the OJT documented training in the high complexity test method. This is my interpretation and how it has been applied to me at various employers anyhow. AS Degree or equivalent- must have unless grandfathered Training-formal or OJT documented OR 3 months in high complexity Joelle Weaver MAOM, HTL (ASCP) QIHC > From: vrivera@westderm.com > To: joelleweaver@hotmail.com > Subject: RE: [Histonet] Clia grossing regulations > Date: Fri, 22 Nov 2013 17:23:33 +0000 > > Hi Joelle, > > I hope you're having a great Friday so far. I'm hoping you can help shed some light on the "OR'' part at the end of #2. At the end of part 2 it states " OR at least 3 months documented laboratory training in each specialty in which the individual performs high complexity testing." Does this mean that an individual without an associate's degree or 60 semester hours can participate in grossing, as long as they have 3 months of documented lab training? > > Regards, > > Vincent Rivera, HT (ASCP), QIHC, QLS > Histopathology Supervisor > West Dermatology Pathology Laboratory > vrivera@westderm.com > 714-924-7240 (Lab) > 714-390-0906 (Cell) > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelle weaver > Sent: Friday, November 22, 2013 5:39 AM > To: Joanne Clark; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Clia grossing regulations > > > > > > The > minimum training/experience required of such personnel is: > > > > > > 1. > An earned associate degree in a laboratory science or medical laboratory technology, obtained from an accredited institution, OR > > > 2. > Education/training equivalent to the above that includes at least 60 semester hours or equivalent from an accredited institution. This education must include > 24 semester hours of medical laboratory technology courses, OR 24 semester hours of science courses that includes 6 semester hours of chemistry, 6 semester hours of biology, and 12 semester hours of chemistry, biology or medical laboratory technology in any combination. In addition, the individual must have laboratory training including either completion of a clinical laboratory training program approved or accredited by the ABHES, the CAHEA, or other organization approved by HHS (note that this training may be included in the > 60 semester hours listed above), OR at least 3 months documented laboratory training in each specialty in which the individual performs high complexity testing. > > > > > > In addition, the > CLIA-88 regulations include exceptions for grandfathered individuals; these regulations (42CFR493.1489 and 1491) may be found at http://www.phppo.cdc.gov/clia/regs/subpart_m.aspx#493.1487 > > > > > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > From: jclark@pcnm.com > > To: histonet@lists.utsouthwestern.edu > > Date: Thu, 21 Nov 2013 18:09:34 +0000 > > Subject: [Histonet] Clia grossing regulations > > > > Carol, here they are: > > > > **REVISED** 07/11/2011 > > ANP.11610 Gross Examination Qualifications Phase II If individuals > > other than a pathologist or pathology resident assist in gross > > examinations, such individuals qualify as high complexity testing personnel under CLIA regulations. > > NOTE: The laboratory director may delegate the dissection of specimens > > to non-pathologist individuals; these individuals must be qualified as > > high complexity testing personnel under CLIA regulations. The minimum training/experience required of such personnel is: > > 1. An earned associate degree in a laboratory science or medical > > laboratory technology, obtained from an accredited institution, OR 2. > > Education/training equivalent to the above that includes at least 60 > > semester hours or equivalent from an accredited institution. This > > education must include 24 semester hours of medical laboratory > > technology courses, OR 24 semester hours of science courses that > > includes 6 semester hours of chemistry, 6 semester hours of biology, > > and > > 12 semester hours of chemistry, biology or medical laboratory > > technology in any combination. In addition, the individual must have > > laboratory training including either completion of a clinical > > laboratory training program approved or accredited by the ABHES, > > NAACLA, or other organization approved by HHS (note that this training > > may be included in the 60 semester hours listed above), OR at least 3 > > months documented laboratory training in each specialty in which the individual performs high complexity testing. > > It is the responsibility of the laboratory director to determine > > whether an individual's education, training and experience satisfies the requirements of this checklist requirement. > > This checklist requirement applies only to laboratories subject to US regulations. > > > > Joanne Clark, HT > > Director of Histology > > Pathology Consultants of New Mexico > > ---------------------------------------------------------------------- > > ---------------- > > > > Message: 4 > > Date: Wed, 20 Nov 2013 21:22:47 -0500 > > From: Carol Kowalcyk > > Subject: [Histonet] what are the Clia regulations for an Ht to > > gross??? > > To: Histonet@lists.utsouthwestern.edu > > Message-ID: <351EA433-CFDA-4D7D-B169-60C4A1483C0D@gmail.com> > > Content-Type: text/plain > > > > > > > > > > * > > > > > > > > Disclaimer: This electronic message may contain information that is > > proprietary, confidential, or legally privileged or protected. It is > > intended only for the use of the individual(s) and entity named in the > > message. If you are not an intended recipient of this message, please > > notify the sender immediately and delete the material from your > > computer. Do not deliver, distribute or copy this message and do not > > disclose its contents or take any action in reliance on the information it contains. > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From madary <@t> verizon.net Fri Nov 22 15:00:03 2013 From: madary <@t> verizon.net (madary@verizon.net) Date: Fri Nov 22 15:00:13 2013 Subject: [Histonet] IHC lab who can do Veterinary Markers? Message-ID: <19619408.886016.1385154003174.JavaMail.root@vznit170128> Looking for a lab that can do IHC on veterinary tissue of all types, rodents(murine and rats), canine, feline, equine, avia as many as a zoo would have.. Slides would be precut, usually with a control already on the slide. Lookng for a lab with high quality IHC, expeditious TAT and a competitive cost. Would prefer DC/MD/VA area with a courier, but mailings will do provided it is not cost prohibitive. For those who are serious, please send information to [1]Joseph.N.Madary@health.mil Very respectfully, Nick(Rocky) Madary, HT/HTL(ASCP)QIHC References 1. mailto:Joseph.N.Madary@health.mil From patrick.lewis <@t> seattlechildrens.org Fri Nov 22 15:40:33 2013 From: patrick.lewis <@t> seattlechildrens.org (Lewis, Patrick) Date: Fri Nov 22 15:40:43 2013 Subject: [Histonet] Trouble with IHC Message-ID: <3903BE18914F4440834F0E620415FFCA381F7626@PPWEXD01a.childrens.sea.kids> Hi everyone I am having some trouble with my IHC on frozen OCT sections. The antibody/secondary/AEC works great on my Epitope retrieved paraffin sections. However, When I try a similar IHC with frozen OCT blocks. Different samples however, but still should be positive. I get lots of nonspecific background staining and no or impossible to tell specific staining when I run them with no epitope retrieval. If I try an epitope retrieval on the OCT sections, I get no background but also no specific staining. My OCT sections are fixed for 30 minutes in 4% p-formaldehyde. Otherwise they are treated pretty much the same way as my paraffin sections (-xylene/etoh of course) Does anyone have a general IHC protocol for frozen sections with a monoclonal mouse and/or polyclonal rabbit primary antibody with a HRP labeled secondary antibody using an AEC substrate? I want to se if my protocol is off the mark at any step that might cause issues. Thanks Patrick. Also, I was cutting my OCT sections at 5 um. The slides look a bit holey and chewed up. (I didn't embed these OCT blocks). I am thinking of cutting 8-10 uM sections next time to put a little more tissue on the slide so that they are a little more robust for IHC. This is my initial attempts to IHC these OCT blocks, so I think a lot of optimization may need to be done, but I was surprised that it would work very well for the paraffin and not at all for the Frozen OCT. CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From tilycyn <@t> auburn.edu Fri Nov 22 16:29:16 2013 From: tilycyn <@t> auburn.edu (Cynthia Hutchinson) Date: Fri Nov 22 16:33:56 2013 Subject: [Histonet] F480 antibody staining Message-ID: <2872A2FD0D55EF4EA941EF72CC1ED6251365BFA7@exmb3> Can anyone share a clean staining protocol utilizing a rat monoclonal (I have rat anti mouse cloneCI:A3-1 from AbD) on mouse tissues? Thanks, Cindy From liz <@t> premierlab.com Fri Nov 22 16:43:38 2013 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Fri Nov 22 16:45:09 2013 Subject: [Histonet] RE: F480 antibody staining In-Reply-To: <2872A2FD0D55EF4EA941EF72CC1ED6251365BFA7@exmb3> References: <2872A2FD0D55EF4EA941EF72CC1ED6251365BFA7@exmb3> Message-ID: <14E2C6176416974295479C64A11CB9AE019C79E01F1D@SBS2K8.premierlab.local> Cindy We use that antibody with dako's rabbit anti-rat (mouse absorbed) and then envision rabbit after that, we use proteinase K for digestion. If you don't want to use the polymer reagent you can use strep avidin instead. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 881-0763 cell (303) 682-9060 fax liz@premierlab.com Ship to address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cynthia Hutchinson [tilycyn@auburn.edu] Sent: Friday, November 22, 2013 3:29 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] F480 antibody staining Can anyone share a clean staining protocol utilizing a rat monoclonal (I have rat anti mouse cloneCI:A3-1 from AbD) on mouse tissues? Thanks, Cindy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rob <@t> foliobio.com Fri Nov 22 16:58:01 2013 From: rob <@t> foliobio.com (Rob Day) Date: Fri Nov 22 16:58:12 2013 Subject: [Histonet] IHC lab who can do Veterinary Markers? Message-ID: <6BACDD6F-68EF-4F8F-9353-874814D4CE54@foliobio.com> You might try www.phylogenyinc.com. > Looking for a lab that can do IHC on veterinary tissue of all types, > rodents(murine and rats), canine, feline, equine, avia as many as a > zoo would have.. Slides would be precut, usually with a control > already on the slide. Lookng for a lab with high quality IHC, > expeditious TAT and a competitive cost. Would prefer DC/MD/VA area > with a courier, but mailings will do provided it is not cost > prohibitive. For those who are serious, please send information to > [1]Joseph.N.Madary@health.mil > > > Very respectfully, > > > Nick(Rocky) Madary, HT/HTL(ASCP)QIHC > > References > > 1. mailto:Joseph.N.Madary@health.mil > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From haemeotoxylin <@t> hotmail.com Fri Nov 22 17:05:33 2013 From: haemeotoxylin <@t> hotmail.com (lisa ryan) Date: Fri Nov 22 17:05:38 2013 Subject: [Histonet] Cleaning stainless steel base molds (Susie Hargrove) Message-ID: we use the histomate filled with paraclear as it only takes approx. 5 minutes. From carl.hobbs <@t> kcl.ac.uk Sat Nov 23 15:24:33 2013 From: carl.hobbs <@t> kcl.ac.uk (Hobbs, Carl) Date: Sat Nov 23 15:24:47 2013 Subject: [Histonet] Re: Cleaning stainless steel base molds Message-ID: I put mine in my 60C oven for a day or longer. Then re-use them....never have a problem... for> 20 yrs. Sure, place on absorbent paper in most appropriate orientation. Sure, when I was in a lab where I was not in charge...I had to use the recycle routine on a processor or...manual xylene clean. Not necessary. From asanjeet <@t> yahoo.com Sun Nov 24 14:33:08 2013 From: asanjeet <@t> yahoo.com (Sanjeet) Date: Sun Nov 24 14:33:14 2013 Subject: [Histonet] Over fixation Message-ID: <445E572B-820F-4714-80C1-CF1925DA4378@yahoo.com> Hi Histo folks, Tissue biopsy that are not fixed properly do have an adverse effect on H & E staining. Tissues can be kept in formalin for a period of 5 to 7 days as per literature. But what happens to the tissues that are kept over for a longer period of time. By that I mean 6 to 12 months. Would over fixed tissues have any effect on H & E stain. Some of my colleagues say NO, but I say YES. I find that the over fixed tissues lose their affinity towards the dyes . Please advice. Sent from my iPhone From Tony.Reilly <@t> health.qld.gov.au Sun Nov 24 17:37:49 2013 From: Tony.Reilly <@t> health.qld.gov.au (Tony Reilly) Date: Sun Nov 24 17:38:33 2013 Subject: [Histonet] Over fixation In-Reply-To: <445E572B-820F-4714-80C1-CF1925DA4378@yahoo.com> References: <445E572B-820F-4714-80C1-CF1925DA4378@yahoo.com> Message-ID: Hi Sanjeet It is true that tissue can bleach over time in formalin making staining difficult. This possibly happens if the formalin becomes acidic over time. This can be improved by treating the tissue with 0.05% periodic acid followed by a water wash and then staining as usual. Regards Tony Tony Reilly B.App.Sc. , M.Sc. Chief Scientist, Anatomical Pathology Pathology Queensland-PA Laboratory ________________________________________________ Health Services Support Agency | Department of Health Level 1, Building 15,Princess Alexandra Hospital Ipswich Road,WOOLLOONGABBA Qld 4102 Ph: 07 3176 2412 Mob: 0402 139411 Fax: 07 3176 2930 Email: tony.reilly@health.qld.gov.au Web: www.health.qld.gov.au/qhcss/ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sanjeet Sent: Monday, 25 November 2013 6:33 AM To: Tony Reilly; histonet@lists.utsouthwestern.edu Subject: [Histonet] Over fixation Hi Histo folks, Tissue biopsy that are not fixed properly do have an adverse effect on H & E staining. Tissues can be kept in formalin for a period of 5 to 7 days as per literature. But what happens to the tissues that are kept over for a longer period of time. By that I mean 6 to 12 months. Would over fixed tissues have any effect on H & E stain. Some of my colleagues say NO, but I say YES. I find that the over fixed tissues lose their affinity towards the dyes . Please advice. Sent from my iPhone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ********************************************************************************** From massimo.tosi_m <@t> libero.it Mon Nov 25 01:11:02 2013 From: massimo.tosi_m <@t> libero.it (massimo.tosi_m@libero.it) Date: Mon Nov 25 01:12:07 2013 Subject: R: RE: [Histonet] Over fixation Message-ID: <1669305742.11764431385363462473.JavaMail.actor@webmail29> It is an ancient method but it works well to avoid that the formalin becomes acid. It owed to the formation of formic acid (HCOOH). It is enough to put i a little piece of marble (CaCO3) nto the solution together with the specimens. (Reference:" Histology Technique" - Dr. Hans-Christian Burck) Kind Regards, Massimo Tosi >----Messaggio originale---- >Da: Tony.Reilly@health.qld.gov.au >Data: 25-nov-2013 0.37 >A: "Sanjeet", "histonet@lists.utsouthwestern.edu" >Ogg: RE: [Histonet] Over fixation > >Hi Sanjeet > >It is true that tissue can bleach over time in formalin making staining difficult. This possibly happens if the formalin becomes acidic over time. This can be improved by treating the tissue with 0.05% periodic acid followed by a water wash and then staining as usual. > >Regards >Tony > > > >Tony Reilly B.App.Sc. , M.Sc. > >Chief Scientist, Anatomical Pathology > >Pathology Queensland-PA Laboratory > >________________________________________________ >Health Services Support Agency | Department of Health > > > >Level 1, Building 15,Princess Alexandra Hospital > >Ipswich Road,WOOLLOONGABBA Qld 4102 >Ph: 07 3176 2412 >Mob: 0402 139411 > >Fax: 07 3176 2930 > >Email: tony.reilly@health.qld.gov.au > >Web: www.health.qld.gov.au/qhcss/ > > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet- bounces@lists.utsouthwestern.edu] On Behalf Of Sanjeet >Sent: Monday, 25 November 2013 6:33 AM >To: Tony Reilly; histonet@lists.utsouthwestern.edu >Subject: [Histonet] Over fixation > >Hi Histo folks, >Tissue biopsy that are not fixed properly do have an adverse effect on H & E staining. Tissues can be kept in formalin for a period of 5 to 7 days as per literature. But what happens to the tissues that are kept over for a longer period of time. By that I mean 6 to 12 months. Would over fixed tissues have any effect on H & E stain. Some of my colleagues say NO, but I say YES. I find that the over fixed tissues lose their affinity towards the dyes . Please advice. > >Sent from my iPhone >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >******************************************************************************** >This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. >Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. >If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. >If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. >Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. >Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. >********************************************************************************** > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From cheastys <@t> svm.vetmed.wisc.edu Mon Nov 25 09:59:06 2013 From: cheastys <@t> svm.vetmed.wisc.edu (Sandra Cheasty) Date: Mon Nov 25 09:58:33 2013 Subject: [Histonet] Issue with Henry's Wonder Wax Enhanced Message-ID: Hello fellow Techs, Has anyone noticed a change in the Henry's Wonder Wax, Enhanced? (Used for embedding, not infiltrating.) We have noticed that it is a lot "stickier" when we are sectioning. (To our picks, forceps, and fingers.) We have checked our temperatures, and all is well. I have asked the company, but they have not replied. Can anyone recommend another embedding paraffin? (We switched from Paraplast Plus about a year ago when we noticed changes after the original manufacturer was bought out.) Thank you, and have a safe food and family filled Thanksgiving! Sandy Sandra Cheasty Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine From gu.lang <@t> gmx.at Mon Nov 25 10:30:19 2013 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Mon Nov 25 10:30:55 2013 Subject: AW: [Histonet] Over fixation In-Reply-To: <445E572B-820F-4714-80C1-CF1925DA4378@yahoo.com> References: <445E572B-820F-4714-80C1-CF1925DA4378@yahoo.com> Message-ID: <000301cee9fb$a3085b20$e9191160$@gmx.at> Formaldehyd binds as methylol-group to basic functional groups on proteins. The longer the fixation the longer the possibility to bind. The same basic groups bind acid dyes like eosin. On the other side leads acidic formalin to degradation of DNA. This can lead to lower affinity of hematoxylin. So in sum long fixed tissue should stain paler. In old literature you find the recommendation to wash fixed tissue in tapwater as long as the fixation has longed. Hmm ;-) Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Sanjeet Gesendet: Sonntag, 24. November 2013 21:33 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Over fixation Hi Histo folks, Tissue biopsy that are not fixed properly do have an adverse effect on H & E staining. Tissues can be kept in formalin for a period of 5 to 7 days as per literature. But what happens to the tissues that are kept over for a longer period of time. By that I mean 6 to 12 months. Would over fixed tissues have any effect on H & E stain. Some of my colleagues say NO, but I say YES. I find that the over fixed tissues lose their affinity towards the dyes . Please advice. Sent from my iPhone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TJohnson <@t> gnf.org Mon Nov 25 10:33:34 2013 From: TJohnson <@t> gnf.org (Teri Johnson) Date: Mon Nov 25 10:33:46 2013 Subject: [Histonet] RE: Trouble with IHC Message-ID: <9F3CFEE76E51B64991C7485270890B40497EA350@EX5.lj.gnf.org> Hi Patrick, It sounds as though you have a lot going on. It's important to gauge whether your frozen sections are moth-eaten due to trimming/facing the tissue too aggressively and tearing chunks of tissue out, if it's freezing artifact, or if your sample is too cold and you are getting chatter artifact. As for your IHC, it could be that your antibody is optimized for formalin fixed, paraffin embedded samples. You can fixing the tissues longer, you can even post fix again after sectioning and before staining and see if that helps. Or it could be that you need to use some heat induced retrieval for it to recognize the protein, and we have successfully used retrieval at 60 degrees C on fixed frozen sections. We avoid the high temp (>= 95 degrees C) as it could cause more damage to the tissue and epitopes. You may also think about post-fixing slides with a formal-alcohol, zinc formalin, or alcohol-acetone and see if any of those work for your sample. Do you have an antibody that is known to work in frozen section? If so, use this to optimize your detection system. Once that is optimized, it is easier to then optimize staining conditions with unknown antibodies. Which antibody are you trying to stain and what tissue are you using? Best wishes, Teri Johnson Manager, Histology Genomics Institute for Novartis Research Foundation San Diego, CA 858-332-4752 From Vickroy.Jim <@t> mhsil.com Mon Nov 25 10:43:31 2013 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Mon Nov 25 10:43:37 2013 Subject: [Histonet] Reagent change on tissue processors Message-ID: In the past we had a fewer number of processors and fewer variables so we based changing our chemicals on the day of the week. Now since we have more machines for better workflow purposes we believe changing should be based on number of cassettes run through each machine. Of course then we have to figure number of cassettes with processing sponges and should the number of cassettes be different on the machine we run our fatty tissue. I have talked to a few people and they change their machines after anywhere from 300 - 600 cassettes. I know we need to establish the best setup for ourselves but it would be nice to hear what others are doing. We've come a long way from the past where every Wednesday we dumped all of the reagents and including all of the 100%'s and the clearing agents. (We rotate them now every other day.) Thanks for your input. James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From jaylundgren <@t> gmail.com Mon Nov 25 16:12:06 2013 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Mon Nov 25 16:12:13 2013 Subject: [Histonet] Reagent change on tissue processors In-Reply-To: References: Message-ID: For registry quality slides, rotate everything except paraffin every 200 blocks (basically every day unless you do tiny runs). Rotate paraffin weekly, sooner if it starts smelling strongly of clearant. But then, I'm conservative, and I was trained where reagents were free. Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) On Mon, Nov 25, 2013 at 10:43 AM, Vickroy, Jim wrote: > > In the past we had a fewer number of processors and fewer variables so we > based changing our chemicals on the day of the week. Now since we have > more machines for better workflow purposes we believe changing should be > based on number of cassettes run through each machine. Of course then we > have to figure number of cassettes with processing sponges and should the > number of cassettes be different on the machine we run our fatty tissue. > I have talked to a few people and they change their machines after > anywhere from 300 - 600 cassettes. I know we need to establish the best > setup for ourselves but it would be nice to hear what others are doing. > We've come a long way from the past where every Wednesday we dumped all of > the reagents and including all of the 100%'s and the clearing agents. (We > rotate them now every other day.) > > Thanks for your input. > > James Vickroy BS, HT(ASCP) > > Surgical and Autopsy Pathology Technical Supervisor > Memorial Medical Center > 217-788-4046 > > > ________________________________ > This message (including any attachments) contains confidential information > intended for a specific individual and purpose, and is protected by law. If > you are not the intended recipient, you should delete this message. Any > disclosure, copying, or distribution of this message, or the taking of any > action based on it, is strictly prohibited. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From michael <@t> mcevoyandfarmer.com Tue Nov 26 00:12:27 2013 From: michael <@t> mcevoyandfarmer.com (Michael Farmer) Date: Tue Nov 26 00:12:29 2013 Subject: [Histonet] Dako Omnis Message-ID: <88AE0AAB-5ED0-4388-99F8-E71DEED7CF12@mcevoyandfarmer.com> May I ask please, has anyone heard how many Dako Omnis systems are out there? And where? Who are these brave early adaptors? Thanks, Michael Farmer McEvoy & Farmer Pathology NY, NY From gagnone <@t> KGH.KARI.NET Tue Nov 26 08:51:06 2013 From: gagnone <@t> KGH.KARI.NET (Gagnon, Eric) Date: Tue Nov 26 08:51:18 2013 Subject: [Histonet] Dako Omnis Message-ID: <5F06C3AD0B27264CA20CFA986C87882E9E63DA0A@EXCHANGEPV1.KGH.ON.CA> London, Ontario. Eric Gagnon MLT Histology Laboratory Kingston General Hospital Kingston, Ontario, Canada From Doug.Geddes <@t> lhsc.on.ca Tue Nov 26 10:35:17 2013 From: Doug.Geddes <@t> lhsc.on.ca (Doug Geddes) Date: Tue Nov 26 10:35:34 2013 Subject: [Histonet] Dako Omnis In-Reply-To: <5F06C3AD0B27264CA20CFA986C87882E9E63DA0A@EXCHANGEPV1.KGH.ON.CA> References: <5F06C3AD0B27264CA20CFA986C87882E9E63DA0A@EXCHANGEPV1.KGH.ON.CA> Message-ID: <529487750200006100061B7A@lhscgwiao.lhsc.on.ca> Demo only. >>> "Gagnon, Eric" 2013-11-26 9:51 AM >>> London, Ontario. Eric Gagnon MLT Histology Laboratory Kingston General Hospital Kingston, Ontario, Canada _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------------- This information is directed in confidence solely to the person named above and may contain confidential and/or privileged material. This information may not otherwise be distributed, copied or disclosed. If you have received this e-mail in error, please notify the sender immediately via a return e-mail and destroy original message. Thank you for your cooperation. From Richard.Cartun <@t> hhchealth.org Wed Nov 27 09:06:44 2013 From: Richard.Cartun <@t> hhchealth.org (Cartun, Richard) Date: Wed Nov 27 09:06:51 2013 Subject: [Histonet] S-100 mAb Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E01896BA4@HHCEXCHMB05.hhcsystem.org> Can anyone recommend a monoclonal S-100 antibody for IHC on FFPE tissue? We have been using clone "4C4.9"; however, it cross-reacts with muscle. Happy Thanksgiving to all my Histonet colleagues. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax richard.cartun@hhchealth.org This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From candice_camille <@t> yahoo.com Wed Nov 27 10:45:48 2013 From: candice_camille <@t> yahoo.com (Candice Smoots) Date: Wed Nov 27 10:45:52 2013 Subject: [Histonet] IHC lab who can do Veterinary Markers? In-Reply-To: <6BACDD6F-68EF-4F8F-9353-874814D4CE54@foliobio.com> References: <6BACDD6F-68EF-4F8F-9353-874814D4CE54@foliobio.com> Message-ID: <1385570748.24936.YahooMailNeo@web165003.mail.bf1.yahoo.com> You should try Idexx Laboratories! I remain yours truely, Candice Camille ________________________________ From: Rob Day To: histonet@lists.utsouthwestern.edu Sent: Friday, November 22, 2013 4:58 PM Subject: [Histonet] IHC lab who can do Veterinary Markers? You might try www.phylogenyinc.com. >? ? Looking? for a lab that can do IHC on veterinary tissue of all types, >? rodents(murine? and? rats),? canine, feline, equine, avia as many as a >? zoo? would? have..? Slides? would? be? precut,? usually with a control >? already? on? the? slide.? Lookng? for? a? lab? with? high quality IHC, >? expeditious? TAT? and? a? competitive cost. Would prefer DC/MD/VA area >? with? a? courier,? but? mailings? will? do? provided? it? is? not cost >? prohibitive.? For? those? who? are serious, please send information to >? [1]Joseph.N.Madary@health.mil > > >? Very respectfully, > > >? Nick(Rocky) Madary, HT/HTL(ASCP)QIHC > > References > >? 1. mailto:Joseph.N.Madary@health.mil > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cls71877 <@t> gmail.com Wed Nov 27 11:42:08 2013 From: cls71877 <@t> gmail.com (Cristi Rigazio) Date: Wed Nov 27 11:42:20 2013 Subject: [Histonet] IHC lab who can do Veterinary Markers? In-Reply-To: <1385570748.24936.YahooMailNeo@web165003.mail.bf1.yahoo.com> References: <6BACDD6F-68EF-4F8F-9353-874814D4CE54@foliobio.com> <1385570748.24936.YahooMailNeo@web165003.mail.bf1.yahoo.com> Message-ID: <440EDEEA-167A-4762-B228-0CAACF6A8634@gmail.com> MPI does as well. Melissa Martinek at 269-668-3336 ext 1271 can get you where you need to be. Sent from my iPhone On Nov 27, 2013, at 8:45 AM, Candice Smoots wrote: > You should try Idexx Laboratories! > > > I remain yours truely, > > Candice Camille > > > ________________________________ > From: Rob Day > To: histonet@lists.utsouthwestern.edu > Sent: Friday, November 22, 2013 4:58 PM > Subject: [Histonet] IHC lab who can do Veterinary Markers? > > > You might try www.phylogenyinc.com. > > >> Looking for a lab that can do IHC on veterinary tissue of all types, >> rodents(murine and rats), canine, feline, equine, avia as many as a >> zoo would have.. Slides would be precut, usually with a control >> already on the slide. Lookng for a lab with high quality IHC, >> expeditious TAT and a competitive cost. Would prefer DC/MD/VA area >> with a courier, but mailings will do provided it is not cost >> prohibitive. For those who are serious, please send information to >> [1]Joseph.N.Madary@health.mil >> >> >> Very respectfully, >> >> >> Nick(Rocky) Madary, HT/HTL(ASCP)QIHC >> >> References >> >> 1. mailto:Joseph.N.Madary@health.mil >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sdysart <@t> mirnarx.com Wed Nov 27 13:13:05 2013 From: sdysart <@t> mirnarx.com (Sarah Dysart) Date: Wed Nov 27 13:13:13 2013 Subject: [Histonet] See You Soon Message-ID: <488dd7f2012c47899d4a47bfba5c97e3@BY2PR07MB106.namprd07.prod.outlook.com> Hey guys and gals...I have taken a new position as Pathology Supervisor at North Austin Medical Center here in Austin...so I am unsubscribing for now, but have no fear...I will be back ASAP with my new email address. Hope everyone has a great Turkey Day!! Signing off for now... Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 From hmarlatt26 <@t> gmail.com Wed Nov 27 13:32:22 2013 From: hmarlatt26 <@t> gmail.com (Heather Marlatt) Date: Wed Nov 27 13:32:26 2013 Subject: [Histonet] See You Soon In-Reply-To: <488dd7f2012c47899d4a47bfba5c97e3@BY2PR07MB106.namprd07.prod.outlook.com> References: <488dd7f2012c47899d4a47bfba5c97e3@BY2PR07MB106.namprd07.prod.outlook.com> Message-ID: CONGRATULATIONS SARAH!!!!! I hope you love the new position! :-) On Wed, Nov 27, 2013 at 11:13 AM, Sarah Dysart wrote: > Hey guys and gals...I have taken a new position as Pathology Supervisor at > North Austin Medical Center here in Austin...so I am unsubscribing for now, > but have no fear...I will be back ASAP with my new email address. Hope > everyone has a great Turkey Day!! > > Signing off for now... > > Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) > Histotechnologist > Mirna Therapeutics > 2150 Woodward Street > Suite 100 > Austin, Texas 78744 > (512)901-0900 ext. 6912 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From relia1 <@t> earthlink.net Wed Nov 27 13:37:05 2013 From: relia1 <@t> earthlink.net (Pam Barker) Date: Wed Nov 27 13:37:17 2013 Subject: [Histonet] OT: Happy Thanksgiving Message-ID: <02b201ceeba8$128481b0$378d8510$@earthlink.net> Happy Thanksgiving Everyone. From Pam Barker? and RELIA Solutions! Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From avistarop <@t> ffyb.uba.ar Wed Nov 27 13:54:35 2013 From: avistarop <@t> ffyb.uba.ar (avistarop@ffyb.uba.ar) Date: Wed Nov 27 13:55:05 2013 Subject: [Histonet] TIA1 and granzyme B Message-ID: <6a3cdcbca6cb3e75e2c0c7938854a836.squirrel@huemul.ffyb.uba.ar> Hello to everyone! Has anyone used -Mouse Monoclonal [TIA-1] to TIA1 (ab2712) and - Mouse anti human granzyme B (Specificity: granzyme B) both on FFPE tissue? Could I please get a copy of your protocols? Thank you so much. Aldana Vistarop Laboratorio Biolog?a Molecular, Servicio de Anatom?a Patol?gica, Hospital de Ni?os Ricardo Guti?rrez. Gallo 1330 C1425EFD Ciudad de Buenos Aires Argentina TE: (5411) 4962-9138. FAX: (5411)4962-9138. From Tom_Wells <@t> bcit.ca Wed Nov 27 14:10:36 2013 From: Tom_Wells <@t> bcit.ca (Tom Wells) Date: Wed Nov 27 14:10:45 2013 Subject: [Histonet] Rate of microtome injuries in Histotechnology schools. Message-ID: Hi, I am curious about the rate of microtome cuts that students receive in Histotechnology schools. We have a rather large program and take in approximately 60 (or more) students a year. Our Histotechnology semester is around 20 weeks long. During that time our students cut approximately 300 - 400 blocks per student. We typically have approximately 10 minor cuts per semester. How does this compare to other schools? Please email or call me directly to discuss this further. Thank you very much. Tom Tom Wells BSc, MEd, MLT, ART Faculty Department of Medical Laboratory Sciences, School of Health British Columbia Institute of Technology SW03-3088, 3700 Willingdon Avenue Burnaby, BC, V5G 3H2 E Tom_Wells@bcit.ca T (604) 412-7594 C (778) 228 -4102 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Wed Nov 27 14:33:33 2013 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Wed Nov 27 14:33:47 2013 Subject: [Histonet] Supervisor position, Histology, UC San Francisco Medical Center In-Reply-To: <761E2B5697F795489C8710BCC72141FF0B1416@ex07.net.ucsf.edu> References: <761E2B5697F795489C8710BCC72141FF0B1416@ex07.net.ucsf.edu> Message-ID: <761E2B5697F795489C8710BCC72141FF0B4AAC@ex07.net.ucsf.edu> We are still accepting applications for this position. The Histology Supervisor position has opened at UC San Francisco Medical Center in San Francisco, CA Some basic info: Supervises Histology, special stains and IHC. 13 histotechs 30 pathologists, 25 residents, 3 certified PA's, 6 histotech PA's Average 500 blocks/day, range is 300 to 900 Recuts = average 250 per day, up to 500 IHC = 300 slides per day, 230 antibodies on the menu 50 special stains per day 2 peloris processors, 3 VIP5 3 sakura embedding centers 9 Leica 2255 microtomes 1 Sakura Prisma H&E stainer 2 Sakura Glas coverslippers 4 Leica Bond Max IHC stainers, 1 Ventana Ultra Cerner Copath Plus LIS, version 2012, upgrading to version 2013 in the spring. Implementing Copath AB&T barcoding at this time. Go-live is in February. Please apply through the Jobs website: https://careers.ucsfmedicalcenter.org:8443/psp/hcmprd_cg/EMPLOYEE/HRMS/c/HRS_HRAM.HRS_CE.GBL?Page=HRS_CE_JOB_DTL&Action=A&SiteId=1&JobOpeningId=5546&PostingSeq=2 You can send a resume to me at tim.morken@ucsfmedctr.org so we can be sure HR passes your application on to us. If you have any questions, ask me. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center 505 Parnassus Ave, Box 1656 Room S570 San Francisco, CA 94143 (415) 353-1266 (ph) (415) 514-3403 (fax) tim.morken@ucsfmedctr.org Job Description Job ID: 5546 Job Title: HISTOLOGY AND IPOX SUPERVISOR Job Code: 7902 Department: Pathology-Surgical / Histology Location: Parnassus Full/Part Time: Full-Time Appointment Type: Career Shift: Variable Variable Weekly Hours: 40 ; 100% Union Information: This classification is not represented by a union Our legacy of unsurpassed patient care and unceasing mission to integrate high-tech medical research with clinical operations has led to our prestigious standing as one of the top 10 hospitals in the nation according to U.S. News & World Report. As a premier health care institution dedicated to advancing health worldwide, UCSF Medical Center can also be the best place to advance and shape your career. The medical center's employees are one of the most important reasons why we are recognized as one of the nation's best hospitals. To work at UCSF Medical Center is to be part of an institution that provides the highest caliber of care to patients; a nurturing, dynamic and team-oriented atmosphere in which to best use your skills and talents. Job Summary Under general direction from the Medical Director, the incumbent serves as supervisor to 3 Lead Histotechnologists and 9 Histotechnologists. In addition to supervisory duties, the incumbent serves as a technical expert providing direction to staff as well as performing all technical aspects of surgical histology and immunohistochemistry procedures. As supervisor: recruits, hires, trains, completes performance evaluations, and resolves employee issues. Provides orientation, completes competency assessments, maintains staff schedules, training and compliance documentation. Implements new tests and procedures as requested by Medical Directors. Updates and maintains the lab manuals and annual reviews required for accreditation. Integrates new technologies into the laboratory to include analyzing and selecting of new equipment that meets space constraints and operational needs. Ensures that new equipment and associated validation and staff training are performed. Ensures that all equipment is well-maintained. Ensures that staff training and documentation are completed as required. Ensures that QC documentation is complete and quality standards are maintained in the laboratory. Advises and assists researchers planning research projects and determines the ability of the laboratory to accommodate research projects, updating the Medical Director as required. Other duties as assigned. Required Qualifications * College degree in a biological science, chemistry or a related field or equivalent education, Work experience experience as a histotechnologist in a comparable high-volume hospital histology laboratory within the last seven years, including senior-level experience. * Demonstrated high-volume, high-quality sectioning and staining skills. * Ability to organize and prioritize responsibilities and perform well under pressure to meet deadlines. * Excellent interpersonal and communication skills. * ASCP certification: HT licensed or eligible required (candidate will be expected to obtain certification within 1 year), HTL desirable. * Demonstrated ability to organize and prioritize responsibilities and perform well under pressure to meet deadlines required. * Previous, recent supervisory experience in a Histology and /or Immunoperoxidase Laboratory required. * Must be experienced with information technology systems and the use of hardware and software in business and laboratory settings. * Must be an effective user of spreadsheets and database software. Preferred Qualifications * ASCP certification * HT or HTL-licensed strongly preferred. * Previous leadership experience in a hospital laboratory. Licensure/Certification N/A Equal Employment Opportunity UCSF is an Equal Opportunity/Affirmative Action Employer. All qualified applicants are encouraged to apply. Further information about the University of California, San Francisco, is available at diversity.ucsf.edu. UCSF seeks candidates whose skills, and personal and professional experience, have prepared them to contribute to our commitment to diversity and excellence, and the communities we serve. From CObregon <@t> mhs.net Wed Nov 27 17:34:29 2013 From: CObregon <@t> mhs.net (Obregon, Cecilia) Date: Wed Nov 27 17:37:50 2013 Subject: [Histonet] TIA1 and granzyme B In-Reply-To: <6a3cdcbca6cb3e75e2c0c7938854a836.squirrel@huemul.ffyb.uba.ar> References: <6a3cdcbca6cb3e75e2c0c7938854a836.squirrel@huemul.ffyb.uba.ar> Message-ID: <598BB4A3A92D7F4DAA14C30E4AE70C2F27BC8E4E@MHSEXMB05.mhs.net> We are in the completing our validation for both TIA-1 and Granzyme B. Here's what we are using: TIA (EP243) Rabbit Monoclonal from Cell Marque (Predilute, ready to use) Protocol for Ventana Ultra is: Extended Retrieval CC1 (64 minutes), antibody incubation 40 minutes, at 37 degrees with amplification. Granzyme B (Polyclonal) from Cell Marque Catalog#760-4283 Protocol for Ventana Ultra: Mild CC1, at 37 degrees, antibody incubation 16 minutes. Hope that helps! Happy Thanksgiving Cecilia M. Obregon Memorial Regional Hospital 3501 Johnson Street Hollywood, FL 33021 Ph: 954-265-5317 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of avistarop@ffyb.uba.ar [avistarop@ffyb.uba.ar] Sent: Wednesday, November 27, 2013 2:54 PM To: Bell, Pat Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] TIA1 and granzyme B Hello to everyone! Has anyone used -Mouse Monoclonal [TIA-1] to TIA1 (ab2712) and - Mouse anti human granzyme B (Specificity: granzyme B) both on FFPE tissue? Could I please get a copy of your protocols? Thank you so much. Aldana Vistarop Laboratorio Biolog?a Molecular, Servicio de Anatom?a Patol?gica, Hospital de Ni?os Ricardo Guti?rrez. Gallo 1330 C1425EFD Ciudad de Buenos Aires Argentina TE: (5411) 4962-9138. FAX: (5411)4962-9138. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: DO NOT FORWARD THIS MESSAGE TO OTHERS WITHOUT PERMISSION OF THE SENDER. This e-mail, including any attachments, may contain confidential or privileged material that is exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, dissemination, copying, or taking any action in reliance on its contents is prohibited. If you have any reason to believe this e-mail was not intended for you, please delete the e-mail and any attachments, and notify the sender immediately. From avistarop <@t> ffyb.uba.ar Thu Nov 28 06:58:02 2013 From: avistarop <@t> ffyb.uba.ar (avistarop@ffyb.uba.ar) Date: Thu Nov 28 06:58:28 2013 Subject: [Histonet] TIA1 and granzyme B In-Reply-To: <598BB4A3A92D7F4DAA14C30E4AE70C2F27BC8E4E@MHSEXMB05.mhs.net> References: <6a3cdcbca6cb3e75e2c0c7938854a836.squirrel@huemul.ffyb.uba.ar> <598BB4A3A92D7F4DAA14C30E4AE70C2F27BC8E4E@MHSEXMB05.mhs.net> Message-ID: <3cb19b1540694839a46614b9da0ad878.squirrel@huemul.ffyb.uba.ar> Thank you for information. I will Contact you if I have a doubt. Thank you so much!!!! Aldana > We are in the completing our validation for both TIA-1 and Granzyme B. > Here's what we are using: > > TIA (EP243) Rabbit Monoclonal from Cell Marque (Predilute, ready to use) > Protocol for Ventana Ultra is: Extended Retrieval CC1 (64 minutes), > antibody incubation 40 minutes, at 37 degrees with amplification. > > Granzyme B (Polyclonal) from Cell Marque Catalog#760-4283 > Protocol for Ventana Ultra: Mild CC1, at 37 degrees, antibody incubation > 16 minutes. > > Hope that helps! > Happy Thanksgiving > > Cecilia M. Obregon > Memorial Regional Hospital > 3501 Johnson Street > Hollywood, FL 33021 > Ph: 954-265-5317 > > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu > [histonet-bounces@lists.utsouthwestern.edu] on behalf of > avistarop@ffyb.uba.ar [avistarop@ffyb.uba.ar] > Sent: Wednesday, November 27, 2013 2:54 PM > To: Bell, Pat > Cc: histonet@lists.utsouthwestern.edu > Subject: [Histonet] TIA1 and granzyme B > > Hello to everyone! > > Has anyone used > -Mouse Monoclonal [TIA-1] to TIA1 (ab2712) > and > - Mouse anti human granzyme B (Specificity: granzyme B) > both on FFPE tissue? > Could I please get a copy of your protocols? > > Thank you so much. > > Aldana Vistarop > Laboratorio Biolog?a Molecular, Servicio de Anatom?a Patol?gica, > Hospital de Ni?os Ricardo Guti?rrez. > Gallo 1330 > C1425EFD > Ciudad de Buenos Aires > Argentina > TE: (5411) 4962-9138. > FAX: (5411)4962-9138. > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > CONFIDENTIALITY NOTICE: DO NOT FORWARD THIS MESSAGE TO OTHERS WITHOUT > PERMISSION OF THE SENDER. > > This e-mail, including any attachments, may contain confidential or > privileged material that is exempt from disclosure under applicable law. > Any unauthorized review, use, disclosure, dissemination, copying, or > taking any action in reliance on its contents is prohibited. If you have > any reason to believe this e-mail was not intended for you, please delete > the e-mail and any attachments, and notify the sender immediately. > From avistarop <@t> ffyb.uba.ar Thu Nov 28 10:37:52 2013 From: avistarop <@t> ffyb.uba.ar (avistarop@ffyb.uba.ar) Date: Thu Nov 28 10:38:25 2013 Subject: [Histonet] We need your votes!!! to win 15000US$ In-Reply-To: <598BB4A3A92D7F4DAA14C30E4AE70C2F27BC8E4E@MHSEXMB05.mhs.net> References: <6a3cdcbca6cb3e75e2c0c7938854a836.squirrel@huemul.ffyb.uba.ar> <598BB4A3A92D7F4DAA14C30E4AE70C2F27BC8E4E@MHSEXMB05.mhs.net> Message-ID: <8bf68a8b438311beb6ce0174bd31ab34.squirrel@huemul.ffyb.uba.ar> I belong to a group of researchers in one of the biggest hospitals in Buenos Aires - Argentina. Own lab is participating in a contest that can help them win enough money to make the long needed repairs and enhancements it needs. They are participating with a paper they published this year in the Internal Journal of Cancer. The winner of the contest will be the paper that gets the most votes. WE NEED YOUR HELP!! 1) Go to http://www.abcam15.com/login 2) Create an account 3) Once logged in go to Home 4)they will be asked for password confirmation from your mail box, and from your mail they go... 4) to 15th anniversary contest and follow the link to "Vote Now" 5) Their paper is: "Epstein?Barr virus presence in pediatric diffuse large B-cell lymphoma reveals a particular association and latency patterns: Analysis of viral role in tumor microenvironment" by Melina Cohen 6) Click Vote Please help us and the hospital! Thank you so much!!! Login www.abcam15.com From DianaRip1 <@t> aol.com Thu Nov 28 18:25:23 2013 From: DianaRip1 <@t> aol.com (DianaRip1@aol.com) Date: Thu Nov 28 18:25:35 2013 Subject: [Histonet] Re: Histonet Digest, Vol 120, Issue 32 Message-ID: <6cf7b.216f43ab.3fc938f3@aol.com> _Timothy.Morken@ucsfmedctr.org_ (mailto:Timothy.Morken@ucsfmedctr.org) Any idea on Salary? In a message dated 11/28/2013 10:00:35 A.M. Pacific Standard Time, histonet-request@lists.utsouthwestern.edu writes: Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. See You Soon (Sarah Dysart) 2. Re: See You Soon (Heather Marlatt) 3. OT: Happy Thanksgiving (Pam Barker) 4. TIA1 and granzyme B (avistarop@ffyb.uba.ar) 5. Rate of microtome injuries in Histotechnology schools. (Tom Wells) 6. Supervisor position, Histology, UC San Francisco Medical Center (Morken, Timothy) 7. RE: TIA1 and granzyme B (Obregon, Cecilia) 8. RE: TIA1 and granzyme B (avistarop@ffyb.uba.ar) 9. We need your votes!!! to win 15000US$ (avistarop@ffyb.uba.ar) ---------------------------------------------------------------------- Message: 1 Date: Wed, 27 Nov 2013 19:13:05 +0000 From: Sarah Dysart Subject: [Histonet] See You Soon To: "histonet@lists.utsouthwestern.edu" Message-ID: <488dd7f2012c47899d4a47bfba5c97e3@BY2PR07MB106.namprd07.prod.outlook.com> Content-Type: text/plain; charset="us-ascii" Hey guys and gals...I have taken a new position as Pathology Supervisor at North Austin Medical Center here in Austin...so I am unsubscribing for now, but have no fear...I will be back ASAP with my new email address. Hope everyone has a great Turkey Day!! Signing off for now... Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ------------------------------ Message: 2 Date: Wed, 27 Nov 2013 11:32:22 -0800 From: Heather Marlatt Subject: Re: [Histonet] See You Soon To: Sarah Dysart Cc: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=ISO-8859-1 CONGRATULATIONS SARAH!!!!! I hope you love the new position! :-) On Wed, Nov 27, 2013 at 11:13 AM, Sarah Dysart wrote: > Hey guys and gals...I have taken a new position as Pathology Supervisor at > North Austin Medical Center here in Austin...so I am unsubscribing for now, > but have no fear...I will be back ASAP with my new email address. Hope > everyone has a great Turkey Day!! > > Signing off for now... > > Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) > Histotechnologist > Mirna Therapeutics > 2150 Woodward Street > Suite 100 > Austin, Texas 78744 > (512)901-0900 ext. 6912 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 3 Date: Wed, 27 Nov 2013 14:37:05 -0500 From: "Pam Barker" Subject: [Histonet] OT: Happy Thanksgiving To: "Histonet" Message-ID: <02b201ceeba8$128481b0$378d8510$@earthlink.net> Content-Type: text/plain; charset="UTF-8" Happy Thanksgiving Everyone. From Pam Barker??? and RELIA Solutions! Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia ------------------------------ Message: 4 Date: Wed, 27 Nov 2013 16:54:35 -0300 (ART) From: avistarop@ffyb.uba.ar Subject: [Histonet] TIA1 and granzyme B To: "Bell, Pat" Cc: "histonet@lists.utsouthwestern.edu" Message-ID: <6a3cdcbca6cb3e75e2c0c7938854a836.squirrel@huemul.ffyb.uba.ar> Content-Type: text/plain;charset=iso-8859-1 Hello to everyone! Has anyone used -Mouse Monoclonal [TIA-1] to TIA1 (ab2712) and - Mouse anti human granzyme B (Specificity: granzyme B) both on FFPE tissue? Could I please get a copy of your protocols? Thank you so much. Aldana Vistarop Laboratorio Biolog?a Molecular, Servicio de Anatom?a Patol?gica, Hospital de Ni?os Ricardo Guti?rrez. Gallo 1330 C1425EFD Ciudad de Buenos Aires Argentina TE: (5411) 4962-9138. FAX: (5411)4962-9138. ------------------------------ Message: 5 Date: Wed, 27 Nov 2013 20:10:36 +0000 From: Tom Wells Subject: [Histonet] Rate of microtome injuries in Histotechnology schools. To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Hi, I am curious about the rate of microtome cuts that students receive in Histotechnology schools. We have a rather large program and take in approximately 60 (or more) students a year. Our Histotechnology semester is around 20 weeks long. During that time our students cut approximately 300 - 400 blocks per student. We typically have approximately 10 minor cuts per semester. How does this compare to other schools? Please email or call me directly to discuss this further. Thank you very much. Tom Tom Wells BSc, MEd, MLT, ART Faculty Department of Medical Laboratory Sciences, School of Health British Columbia Institute of Technology SW03-3088, 3700 Willingdon Avenue Burnaby, BC, V5G 3H2 E Tom_Wells@bcit.ca T (604) 412-7594 C (778) 228 -4102 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Wed, 27 Nov 2013 20:33:33 +0000 From: "Morken, Timothy" Subject: [Histonet] Supervisor position, Histology, UC San Francisco Medical Center To: Histonet Message-ID: <761E2B5697F795489C8710BCC72141FF0B4AAC@ex07.net.ucsf.edu> Content-Type: text/plain; charset=us-ascii We are still accepting applications for this position. The Histology Supervisor position has opened at UC San Francisco Medical Center in San Francisco, CA Some basic info: Supervises Histology, special stains and IHC. 13 histotechs 30 pathologists, 25 residents, 3 certified PA's, 6 histotech PA's Average 500 blocks/day, range is 300 to 900 Recuts = average 250 per day, up to 500 IHC = 300 slides per day, 230 antibodies on the menu 50 special stains per day 2 peloris processors, 3 VIP5 3 sakura embedding centers 9 Leica 2255 microtomes 1 Sakura Prisma H&E stainer 2 Sakura Glas coverslippers 4 Leica Bond Max IHC stainers, 1 Ventana Ultra Cerner Copath Plus LIS, version 2012, upgrading to version 2013 in the spring. Implementing Copath AB&T barcoding at this time. Go-live is in February. Please apply through the Jobs website: https://careers.ucsfmedicalcenter.org:8443/psp/hcmprd_cg/EMPLOYEE/HRMS/c/HRS _HRAM.HRS_CE.GBL?Page=HRS_CE_JOB_DTL&Action=A&SiteId=1&JobOpeningId=5546&Pos tingSeq=2 You can send a resume to me at tim.morken@ucsfmedctr.org so we can be sure HR passes your application on to us. If you have any questions, ask me. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center 505 Parnassus Ave, Box 1656 Room S570 San Francisco, CA 94143 (415) 353-1266 (ph) (415) 514-3403 (fax) tim.morken@ucsfmedctr.org Job Description Job ID: 5546 Job Title: HISTOLOGY AND IPOX SUPERVISOR Job Code: 7902 Department: Pathology-Surgical / Histology Location: Parnassus Full/Part Time: Full-Time Appointment Type: Career Shift: Variable Variable Weekly Hours: 40 ; 100% Union Information: This classification is not represented by a union Our legacy of unsurpassed patient care and unceasing mission to integrate high-tech medical research with clinical operations has led to our prestigious standing as one of the top 10 hospitals in the nation according to U.S. News & World Report. As a premier health care institution dedicated to advancing health worldwide, UCSF Medical Center can also be the best place to advance and shape your career. The medical center's employees are one of the most important reasons why we are recognized as one of the nation's best hospitals. To work at UCSF Medical Center is to be part of an institution that provides the highest caliber of care to patients; a nurturing, dynamic and team-oriented atmosphere in which to best use your skills and talents. Job Summary Under general direction from the Medical Director, the incumbent serves as supervisor to 3 Lead Histotechnologists and 9 Histotechnologists. In addition to supervisory duties, the incumbent serves as a technical expert providing direction to staff as well as performing all technical aspects of surgical histology and immunohistochemistry procedures. As supervisor: recruits, hires, trains, completes performance evaluations, and resolves employee issues. Provides orientation, completes competency assessments, maintains staff schedules, training and compliance documentation. Implements new tests and procedures as requested by Medical Directors. Updates and maintains the lab manuals and annual reviews required for accreditation. Integrates new technologies into the laboratory to include analyzing and selecting of new equipment that meets space constraints and operational needs. Ensures that new equipment and associated validation and staff training are performed. Ensures that all equipment is well-maintained. Ensures that staff training and documentation are completed as required. Ensures that QC documentation is complete and quality standards are maintained in the laboratory. Advises and assists researchers planning research projects and determines the ability of the laboratory to accommodate research projects, updating the Medical Director as required. Other duties as assigned. Required Qualifications * College degree in a biological science, chemistry or a related field or equivalent education, Work experience experience as a histotechnologist in a comparable high-volume hospital histology laboratory within the last seven years, including senior-level experience. * Demonstrated high-volume, high-quality sectioning and staining skills. * Ability to organize and prioritize responsibilities and perform well under pressure to meet deadlines. * Excellent interpersonal and communication skills. * ASCP certification: HT licensed or eligible required (candidate will be expected to obtain certification within 1 year), HTL desirable. * Demonstrated ability to organize and prioritize responsibilities and perform well under pressure to meet deadlines required. * Previous, recent supervisory experience in a Histology and /or Immunoperoxidase Laboratory required. * Must be experienced with information technology systems and the use of hardware and software in business and laboratory settings. * Must be an effective user of spreadsheets and database software. Preferred Qualifications * ASCP certification * HT or HTL-licensed strongly preferred. * Previous leadership experience in a hospital laboratory. Licensure/Certification N/A Equal Employment Opportunity UCSF is an Equal Opportunity/Affirmative Action Employer. All qualified applicants are encouraged to apply. Further information about the University of California, San Francisco, is available at diversity.ucsf.edu. UCSF seeks candidates whose skills, and personal and professional experience, have prepared them to contribute to our commitment to diversity and excellence, and the communities we serve. ------------------------------ Message: 7 Date: Wed, 27 Nov 2013 23:34:29 +0000 From: "Obregon, Cecilia" Subject: RE: [Histonet] TIA1 and granzyme B To: "avistarop@ffyb.uba.ar" , "Bell, Pat" Cc: "histonet@lists.utsouthwestern.edu" Message-ID: <598BB4A3A92D7F4DAA14C30E4AE70C2F27BC8E4E@MHSEXMB05.mhs.net> Content-Type: text/plain; charset="iso-8859-1" We are in the completing our validation for both TIA-1 and Granzyme B. Here's what we are using: TIA (EP243) Rabbit Monoclonal from Cell Marque (Predilute, ready to use) Protocol for Ventana Ultra is: Extended Retrieval CC1 (64 minutes), antibody incubation 40 minutes, at 37 degrees with amplification. Granzyme B (Polyclonal) from Cell Marque Catalog#760-4283 Protocol for Ventana Ultra: Mild CC1, at 37 degrees, antibody incubation 16 minutes. Hope that helps! Happy Thanksgiving Cecilia M. Obregon Memorial Regional Hospital 3501 Johnson Street Hollywood, FL 33021 Ph: 954-265-5317 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of avistarop@ffyb.uba.ar [avistarop@ffyb.uba.ar] Sent: Wednesday, November 27, 2013 2:54 PM To: Bell, Pat Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] TIA1 and granzyme B Hello to everyone! Has anyone used -Mouse Monoclonal [TIA-1] to TIA1 (ab2712) and - Mouse anti human granzyme B (Specificity: granzyme B) both on FFPE tissue? Could I please get a copy of your protocols? Thank you so much. Aldana Vistarop Laboratorio Biolog?a Molecular, Servicio de Anatom?a Patol?gica, Hospital de Ni?os Ricardo Guti?rrez. Gallo 1330 C1425EFD Ciudad de Buenos Aires Argentina TE: (5411) 4962-9138. FAX: (5411)4962-9138. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: DO NOT FORWARD THIS MESSAGE TO OTHERS WITHOUT PERMISSION OF THE SENDER. This e-mail, including any attachments, may contain confidential or privileged material that is exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, dissemination, copying, or taking any action in reliance on its contents is prohibited. If you have any reason to believe this e-mail was not intended for you, please delete the e-mail and any attachments, and notify the sender immediately. ------------------------------ Message: 8 Date: Thu, 28 Nov 2013 09:58:02 -0300 (ART) From: avistarop@ffyb.uba.ar Subject: RE: [Histonet] TIA1 and granzyme B To: "Obregon, Cecilia" Cc: "histonet@lists.utsouthwestern.edu" Message-ID: <3cb19b1540694839a46614b9da0ad878.squirrel@huemul.ffyb.uba.ar> Content-Type: text/plain;charset=iso-8859-1 Thank you for information. I will Contact you if I have a doubt. Thank you so much!!!! Aldana > We are in the completing our validation for both TIA-1 and Granzyme B. > Here's what we are using: > > TIA (EP243) Rabbit Monoclonal from Cell Marque (Predilute, ready to use) > Protocol for Ventana Ultra is: Extended Retrieval CC1 (64 minutes), > antibody incubation 40 minutes, at 37 degrees with amplification. > > Granzyme B (Polyclonal) from Cell Marque Catalog#760-4283 > Protocol for Ventana Ultra: Mild CC1, at 37 degrees, antibody incubation > 16 minutes. > > Hope that helps! > Happy Thanksgiving > > Cecilia M. Obregon > Memorial Regional Hospital > 3501 Johnson Street > Hollywood, FL 33021 > Ph: 954-265-5317 > > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu > [histonet-bounces@lists.utsouthwestern.edu] on behalf of > avistarop@ffyb.uba.ar [avistarop@ffyb.uba.ar] > Sent: Wednesday, November 27, 2013 2:54 PM > To: Bell, Pat > Cc: histonet@lists.utsouthwestern.edu > Subject: [Histonet] TIA1 and granzyme B > > Hello to everyone! > > Has anyone used > -Mouse Monoclonal [TIA-1] to TIA1 (ab2712) > and > - Mouse anti human granzyme B (Specificity: granzyme B) > both on FFPE tissue? > Could I please get a copy of your protocols? > > Thank you so much. > > Aldana Vistarop > Laboratorio Biolog?a Molecular, Servicio de Anatom?a Patol?gica, > Hospital de Ni?os Ricardo Guti?rrez. > Gallo 1330 > C1425EFD > Ciudad de Buenos Aires > Argentina > TE: (5411) 4962-9138. > FAX: (5411)4962-9138. > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > CONFIDENTIALITY NOTICE: DO NOT FORWARD THIS MESSAGE TO OTHERS WITHOUT > PERMISSION OF THE SENDER. > > This e-mail, including any attachments, may contain confidential or > privileged material that is exempt from disclosure under applicable law. > Any unauthorized review, use, disclosure, dissemination, copying, or > taking any action in reliance on its contents is prohibited. If you have > any reason to believe this e-mail was not intended for you, please delete > the e-mail and any attachments, and notify the sender immediately. > ------------------------------ Message: 9 Date: Thu, 28 Nov 2013 13:37:52 -0300 (ART) From: avistarop@ffyb.uba.ar Subject: [Histonet] We need your votes!!! to win 15000US$ Cc: "histonet@lists.utsouthwestern.edu" Message-ID: <8bf68a8b438311beb6ce0174bd31ab34.squirrel@huemul.ffyb.uba.ar> Content-Type: text/plain;charset=iso-8859-1 I belong to a group of researchers in one of the biggest hospitals in Buenos Aires - Argentina. Own lab is participating in a contest that can help them win enough money to make the long needed repairs and enhancements it needs. They are participating with a paper they published this year in the Internal Journal of Cancer. The winner of the contest will be the paper that gets the most votes. WE NEED YOUR HELP!! 1) Go to http://www.abcam15.com/login 2) Create an account 3) Once logged in go to Home 4)they will be asked for password confirmation from your mail box, and from your mail they go... 4) to 15th anniversary contest and follow the link to "Vote Now" 5) Their paper is: "Epstein?Barr virus presence in pediatric diffuse large B-cell lymphoma reveals a particular association and latency patterns: Analysis of viral role in tumor microenvironment" by Melina Cohen 6) Click Vote Please help us and the hospital! Thank you so much!!! Login www.abcam15.com ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 120, Issue 32 ***************************************** From Ronald.Houston <@t> nationwidechildrens.org Fri Nov 29 08:23:53 2013 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Fri Nov 29 08:24:00 2013 Subject: [Histonet] Tape Transfer system Message-ID: I know that Leica bought over Instrumedics CryoJane Tape Transfer System for frozen sections, but does anyone know where I can get a hold of the paraffin system that Instrumedics had? Thanks Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org "One person with passion is better than forty people merely interested." ~ E.M. Forster From ibernard <@t> uab.edu Fri Nov 29 09:43:42 2013 From: ibernard <@t> uab.edu (Ian R Bernard) Date: Fri Nov 29 09:43:45 2013 Subject: [Histonet] Rush Processing Protocol Message-ID: Fellow Histonetters, our laboratory mostly processes small biopsy specimens such as GI, skins, vas deferens, ECCs, Cervical biopsies, EMBXs and breast core bxs with our largest routine specimen being gall bladders. We are seeking recommendations on a proven same-day or less than 8-hour processing program that would facilitate processing rush specimens only. Of course, we would validate this protocol with our processor and reagents. Any suggestions? Looking for total time and optimum time in each reagent. Our routine overnight is little over a 12 hour protocol. Please provide an evidence-based reference for optimum minimum processing time of biopsy tissues versus maximum time. Below are the reagents and amount of reagent containers we use. 10 % NBF- x 2 70% ALC- x 1 95% ALC- x 1 100% ALC- x 4 Clearing- X 2 Paraffin- X 4 Ian Bernard From Glenn.Hauck <@t> albertahealthservices.ca Fri Nov 29 09:58:24 2013 From: Glenn.Hauck <@t> albertahealthservices.ca (Glenn Hauck) Date: Fri Nov 29 10:02:03 2013 Subject: [Histonet] RE: Rush Processing Protocol In-Reply-To: References: Message-ID: <38FA8FCA474F834CB9B90590D7C72390015A14A48AD2@EXMBX4C.crha.bewell.ca> I am most interested in a rush processes as well, we also only do overnight processing. We have a large variety of both large and small tissue types. The reagents we have on board on our processors are listed below. 10 % NBFx1 70% ALCx1 80%ALCx2 95%ALCx2 100%ALCx2 Xylenex2 Paraffin- X 4 Glenn Hauck -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ian R Bernard Sent: Friday, November 29, 2013 8:44 AM To: histonet@lists.utsouthwestern.edu Cc: BERNARD, IAN R MSgt USAF USAFA 10 MDSS/SGSH Subject: [Histonet] Rush Processing Protocol Fellow Histonetters, our laboratory mostly processes small biopsy specimens such as GI, skins, vas deferens, ECCs, Cervical biopsies, EMBXs and breast core bxs with our largest routine specimen being gall bladders. We are seeking recommendations on a proven same-day or less than 8-hour processing program that would facilitate processing rush specimens only. Of course, we would validate this protocol with our processor and reagents. Any suggestions? Looking for total time and optimum time in each reagent. Our routine overnight is little over a 12 hour protocol. Please provide an evidence-based reference for optimum minimum processing time of biopsy tissues versus maximum time. Below are the reagents and amount of reagent containers we use. 10 % NBF- x 2 70% ALC- x 1 95% ALC- x 1 100% ALC- x 4 Clearing- X 2 Paraffin- X 4 Ian Bernard _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message and any attached documents are only for the use of the intended recipient(s), are confidential and may contain privileged information. Any unauthorized review, use, retransmission, or other disclosure is strictly prohibited. If you have received this message in error, please notify the sender immediately, and then delete the original message. Thank you. From rsrichmond <@t> gmail.com Fri Nov 29 13:03:33 2013 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Fri Nov 29 13:03:38 2013 Subject: [Histonet] Re: Rate of microtome injuries in histotechnology schools Message-ID: Tom Wells BSc, MEd, MLT, ART, Faculty, British Columbia Institute of Technology, Burnaby, BC, Canada notes: >>We have a rather large [histotechnology] program and take in approximately 60 (or more) students a year. Our histotechnology semester is around 20 weeks long.<< 60 a year? Hey, how about sending us a few of them south of the border? The weather's really much better here, if you don't mind dodging a few bullets and dealing with healthcare.gov! Happy Black Friday! Bob Richmond Samurai Pathologist Maryville, Tennessee From Tom_Wells <@t> bcit.ca Fri Nov 29 13:13:45 2013 From: Tom_Wells <@t> bcit.ca (Tom Wells) Date: Fri Nov 29 13:13:54 2013 Subject: [Histonet] Re: Rate of microtome injuries in histotechnology schools In-Reply-To: References: Message-ID: Forget the students; warm sounds good and I could always buy a bullet-proof vest. When do I start! :-) Tom Wells BSc, MEd, MLT, ART Faculty Department of Medical Laboratory Sciences, School of Health British Columbia Institute of Technology SW03-3088, 3700 Willingdon Avenue Burnaby, BC, V5G 3H2 E Tom_Wells@bcit.ca T (604) 412-7594 C (778) 228 -4102 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Friday, November 29, 2013 11:04 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Rate of microtome injuries in histotechnology schools Tom Wells BSc, MEd, MLT, ART, Faculty, British Columbia Institute of Technology, Burnaby, BC, Canada notes: >>We have a rather large [histotechnology] program and take in approximately 60 (or more) students a year. Our histotechnology semester is around 20 weeks long.<< 60 a year? Hey, how about sending us a few of them south of the border? The weather's really much better here, if you don't mind dodging a few bullets and dealing with healthcare.gov! Happy Black Friday! Bob Richmond Samurai Pathologist Maryville, Tennessee _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From chesarato <@t> hotmail.com Sat Nov 30 00:54:48 2013 From: chesarato <@t> hotmail.com (Cesar Francisco Romero) Date: Sat Nov 30 00:54:59 2013 Subject: [Histonet] RE: Rush Processing Protocol Message-ID: I think that what you really need is a Microwave Processor. But I live in a Poor World with No Machines. I have to report small wedge Kidney Biopsies for transplantation in two hours . This is my Rush Processing Protocol: Formalin ( 1 : 10 ) 10 minutes ( they already come from the surgery room in formalin ). Ethyl Alcohol 96? 10 minutes. Ethyl Alcohol 100 ? 10 minutes. Ethyl Alcohol 100 ? 10 minutes. Xylene 15 minutes. Paraffin 15 to 20 minutes. The secret is that I already have all the containers with the reagents in the stove at 62? C and I do all the processing inside the stove. I hope it helps. C?sar RomeroBuenos AiresArgentina