From gu.lang <@t> gmx.at Wed May 1 01:22:11 2013 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Wed May 1 01:22:29 2013 Subject: AW: [Histonet] Monitoring of IHC staining trends In-Reply-To: References: Message-ID: <001e01ce4634$38e8aee0$aaba0ca0$@gmx.at> We use a composed block of normal tissue for very many antibodies as positive control. Theoretically it should be visible, if the staining gets weaker or stronger, if you compare the every-day-the same-picture of the positive control. The problems are, that the observers change, that some antibodies are stained rarely, that the staining-characteristics of some antibodies are not known well.... I think the feedback of the observers is the most important part here, and the comparison to the on-slide-control. Gudrun Lang Ltd. Biomedizinische Analytikerin Histolabor Akh Linz Austria -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Troutman, Kenneth A Gesendet: Dienstag, 30. April 2013 19:28 An: Histonet@lists.utsouthwestern.edu Betreff: [Histonet] Monitoring of IHC staining trends Hello Histonet, I have a question for the group at large. How are labs monitoring drift in IHC staining over time? Here's the scenario: You do lot to lot testing and everything looks fine until one day your pathologists are telling you that the CAM5.2 is too dark. Now, you've been looking at these slides every day for the last year and, sure enough, when you pull out a slide from last year's lot, it is significantly lighter. So what do we do about it? Do we revalidate the stain? Does anyone have a mechanism to monitor this better? What is the threshold for revalidation? Feedback from techs as well as any pathologists would be greatly appreciated. Regards, Ashley Troutman BS, HT(ASCP) QIHC Immunohistochemistry Supervisor Vanderbilt University Histopathology 1301 Medical Center Drive TVC 4531 Nashville, TN 37232 ashley.troutman@vanderbilt.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mjdessoye <@t> commonwealthhealth.net Wed May 1 10:20:09 2013 From: mjdessoye <@t> commonwealthhealth.net (Dessoye, Michael J) Date: Wed May 1 10:20:24 2013 Subject: [Histonet] RE: Histonet Digest, Vol 113, Issue 30 In-Reply-To: <65C8EC869E8581459DCD566079572FAC0B7ABECA@DCPWPEXMBX02.mdanderson.edu> Message-ID: Surgical Pathology Technologist Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdessoye@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 -----Original Message----- From: Barr,Kaye H [mailto:khbarr@mdanderson.org] Sent: Tuesday, April 30, 2013 1:49 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Histonet Digest, Vol 113, Issue 30 What title do most use for someone who does gross and is not a PA? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Tuesday, April 30, 2013 12:02 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 113, Issue 30 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. BIG PROBLEM (Behnaz Sohrab) 2. Re: BIG PROBLEM (Linda) 3. Yearly Training (Nancy Schmitt) 4. HIER pressure cookers.... (k. leigh adams) 5. RE: HIER pressure cookers.... (Connolly, Brett M) 6. RE: HIER pressure cookers.... (Blazek, Linda) 7. Re: HIER pressure cookers.... (Jan Shivers) 8. RE: 5 gal specimen container search (Hannen, Valerie) 9. RE: 5 gal specimen container search (Shirley A. Powell) 10. RE: HIER pressure cookers.... (Dingersoll@aplaboratories.com) 11. AW: [Histonet] BIG PROBLEM (Gudrun Lang) 12. Processor question - alcoholic formalin vs. buffered formalin (Lake, Kim S) 13. RE: Processor question - alcoholic formalin vs. buffered formalin (Hannen, Valerie) 14. cassette labelers (Margiotta-Watz, Michele) ---------------------------------------------------------------------- Message: 1 Date: Mon, 29 Apr 2013 10:36:01 -0700 From: "Behnaz Sohrab" Subject: [Histonet] BIG PROBLEM To: Message-ID: <517E4D11.4347.0054.1@ah.org> Content-Type: text/plain; charset="us-ascii" Good Morning, We have two Leica H&E stainer( old xt-5010 and new 5020). We do use : Gill's 3 for 6 minutes, 4% Glacial ACETIC Acid 40 seconds Bluing : 45 sec. We done this for years!! last few months our Pantologists are complaining about GI Biopsies that they have TOO much blue in cytoplasma and the Mucin is too purple and blue? Please any suggestion?? Mean while we are trying the NEW kit from Leica for H&E , they think that is TOO pale?any of you has tried this new stain from Lecia? With much application. Behnaz Sohrab, Los Angeles ------------------------------ Message: 2 Date: Mon, 29 Apr 2013 11:30:38 -0700 (PDT) From: Linda Subject: Re: [Histonet] BIG PROBLEM To: Behnaz Sohrab , "histonet@lists.utsouthwestern.edu" Message-ID: <1367260238.82176.YahooMailNeo@web164002.mail.gq1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi, ? I would first check with your alcohol and xylene suppliers to see if they changed anything.? When something?goes south it is usually because the manufacturer have changed.? This happened with my silver stains. ? You may have to go back to the basics- harris hematoxylin, acid alcohol, and eosin y.? Richard Allen 7211 hematoxylin and the rest of their staining is excellent in these situations until you can get it back under control.? If you send them slides they will work a time out for you. ? If you are using Leica's selectech, which is what I started this lab out with because I was heavily sampled out,?it has blue nuclei instead of the traditional purple.? I do 5 minutes in hematoxylin, 30 dips in clarifier and 1 minute in blue, 55 sec in eosin but I have (2) 95s after.? They also have a tech dept to help.? I have a Leica autostainer too. ? I hope this helps. ? Linda Dee, HT(ASCP) ? ________________________________ From: Behnaz Sohrab To: histonet@lists.utsouthwestern.edu Sent: Monday, April 29, 2013 12:36 PM Subject: [Histonet] BIG PROBLEM Good Morning, We have two Leica H&E stainer( old xt-5010 and new 5020). We do use : Gill's 3 for 6 minutes, 4% Glacial ACETIC Acid 40 seconds Bluing : 45 sec. We done this for years!! last few months our Pantologists are complaining about GI Biopsies that they have TOO much blue in cytoplasma and the Mucin is too purple and blue? Please any suggestion?? Mean while we are trying the NEW kit from Leica for H&E , they think that is TOO pale?any of you has tried this new stain from Lecia? With much application. Behnaz Sohrab, Los Angeles _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Mon, 29 Apr 2013 18:39:17 +0000 From: Nancy Schmitt Subject: [Histonet] Yearly Training To: "histonet@lists.utsouthwestern.edu" Message-ID: <906B4DA90ED1DB4DB6C7E94D7CEE6C36813C038E@PEITHA.wad.pa-ucl.com> Content-Type: text/plain; charset="us-ascii" Cindy- We also have a yearly competency packet. It follows JCAHO standards and includes direct observations, log documentation for QC and maintenance and problem solving through written exam. Systems covered are H&E, IHC, Special stains, processing/embedding, Cytology prep, and Misc. Histology. Nancy Schmitt Histology Coordinator Dubuque, IA ----------------------------------------------------- From: Cindy Pyse [mailto:cpyse@x-celllab.com] Sent: Friday, April 26, 2013 4:49 PM To: 'histonet-bounces@lists.utsouthwestern.edu' Subject: Yearly training Happy Friday Everyone I just wanted an opinion on what everyone's facilities train for annually. An example is HIPPA training. I know what my regs require, I just wanted to see what everyone else in histoland was doing. Thanks in advance for all responses. I hope everyone has a great weekend. Finally we are going to have a good weather weekend in Western NY. Cindy NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ------------------------------ Message: 4 Date: Tue, 30 Apr 2013 09:29:33 -0400 From: "k. leigh adams" Subject: [Histonet] HIER pressure cookers.... To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Any input as to preferred instruments would be greatly appreciated... Leigh ------------------------------ Message: 5 Date: Tue, 30 Apr 2013 09:53:50 -0400 From: "Connolly, Brett M" Subject: RE: [Histonet] HIER pressure cookers.... To: "k. leigh adams" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Biocare Decloaker here !! Brett M. Connolly, Ph.D. Principal Scientist, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of k. leigh adams Sent: Tuesday, April 30, 2013 9:30 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HIER pressure cookers.... Any input as to preferred instruments would be greatly appreciated... Leigh _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------ Message: 6 Date: Tue, 30 Apr 2013 09:58:58 -0400 From: "Blazek, Linda" Subject: RE: [Histonet] HIER pressure cookers.... To: "Connolly, Brett M" , "k. leigh adams" , "histonet@lists.utsouthwestern.edu" Message-ID: <5A2BD13465E061429D6455C8D6B40E391652B8729D@IBMB7Exchange.digestivespeci alists.com> Content-Type: text/plain; charset="us-ascii" I second that! Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Digestive Specialists, Inc Phone: (937) 396-2623 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Connolly, Brett M Sent: Tuesday, April 30, 2013 9:54 AM To: k. leigh adams; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HIER pressure cookers.... Biocare Decloaker here !! Brett M. Connolly, Ph.D. Principal Scientist, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of k. leigh adams Sent: Tuesday, April 30, 2013 9:30 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HIER pressure cookers.... Any input as to preferred instruments would be greatly appreciated... Leigh _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Tue, 30 Apr 2013 09:34:56 -0500 From: Jan Shivers Subject: Re: [Histonet] HIER pressure cookers.... To: "Blazek, Linda" Cc: "histonet@lists.utsouthwestern.edu" , "k. leigh adams" Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Third that. Jan S On Tue, Apr 30, 2013 at 8:58 AM, Blazek, Linda < lblazek@digestivespecialists.com> wrote: > I second that! > > Linda Blazek HT (ASCP) > Manager/Supervisor > GI Pathology of Dayton > Digestive Specialists, Inc > Phone: (937) 396-2623 > Email: lblazek@digestivespecialists.com > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Connolly, Brett M > Sent: Tuesday, April 30, 2013 9:54 AM > To: k. leigh adams; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] HIER pressure cookers.... > > Biocare Decloaker here !! > > Brett M. Connolly, Ph.D. > Principal Scientist, Imaging Dept. > Merck & Co., Inc. > PO Box 4, WP-44K > West Point, PA 19486 > brett_connolly@merck.com > T- 215-652-2501 > F- 215-993-6803 > > > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of k. leigh adams > Sent: Tuesday, April 30, 2013 9:30 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] HIER pressure cookers.... > > Any input as to preferred instruments would be greatly appreciated... > > Leigh > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Notice: This e-mail message, together with any attachments, contains > information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New > Jersey, USA 08889), and/or its affiliates Direct contact information for > affiliates is available at > http://www.merck.com/contact/contacts.html) that may be confidential, > proprietary copyrighted and/or legally privileged. It is intended solely > for the use of the individual or entity named on this message. If you are > not the intended recipient, and have received this message in error, please > notify us immediately by reply e-mail and then delete it from your system. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Jan Shivers Senior Scientist IHC/Histology Section Head Pathology Teaching Program UMN Veterinary Diagnostic Laboratory University of Minnesota College of Veterinary Medicine 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive003@umn.edu ------------------------------ Message: 8 Date: Tue, 30 Apr 2013 10:36:24 -0400 From: "Hannen, Valerie" Subject: RE: [Histonet] 5 gal specimen container search To: 'Jackie O'Connor' , "histonet@lists.utsouthwestern.edu" Message-ID: <450B7A81EDA0C54E97C53D60F00776C3231B3D7C30@isexstore03> Content-Type: text/plain; charset="us-ascii" Jackie... We have ordered these from a company by the name of Centurian Medical. The lids are sold separately. Valerie -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie O'Connor Sent: Wednesday, April 24, 2013 3:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] 5 gal specimen container search Anyone in histoland know a vendor where I can find a 5 gallon leakproof bucket, drum, pail for shipping a large pathology specimen? Jackie O' _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet =================== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" =================== ------------------------------ Message: 9 Date: Tue, 30 Apr 2013 10:38:59 -0400 From: "Shirley A. Powell" Subject: RE: [Histonet] 5 gal specimen container search To: "Hannen, Valerie" , 'Jackie O'Connor' , "histonet@lists.utsouthwestern.edu" Message-ID: <9BF995BC0E47744E9673A41486E24EE2579B14884C@MERCERMAIL.MercerU.local> Content-Type: text/plain; charset="us-ascii" You can also check with restaurant supply houses, we have purchased great large containers with lids for this purpose. Much cheaper than the lab supply houses. Shirley -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie Sent: Tuesday, April 30, 2013 10:36 AM To: 'Jackie O'Connor'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] 5 gal specimen container search Jackie... We have ordered these from a company by the name of Centurian Medical. The lids are sold separately. Valerie -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie O'Connor Sent: Wednesday, April 24, 2013 3:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] 5 gal specimen container search Anyone in histoland know a vendor where I can find a 5 gallon leakproof bucket, drum, pail for shipping a large pathology specimen? Jackie O' _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ============= "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ============= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Tue, 30 Apr 2013 07:40:51 -0700 From: Subject: RE: [Histonet] HIER pressure cookers.... To: "Blazek, Linda" , "Connolly, Brett M" , "k. leigh adams" , "histonet@lists.utsouthwestern.edu" Message-ID: <20130430074051.073ecbdb5144cf8a05e574ee22bfb11a.114d0c0283.wbe@email17. secureserver.net> Content-Type: text/plain; charset="utf-8" dingersoll@aplaboratories.com -------- Original Message -------- Subject: RE: [Histonet] HIER From: "Blazek, Linda" <[1]lblazek@digestivespecialists.com> To: "Connolly, Brett M" , " adams" <[2]k. "[3]histonet@lists.utsouthwestern.edu" <[4]histonet@lists.utsouthwestern.edu< I second that! Linda Blazek HT (ASCP) GI Pathology of Dayton Digestive Speciali Phone: (937) 396-2623 Email: [5]lblazek@digestivespecialists.com -----Original Message----- From: [6]histonet-bounces@lists.utsouthwes mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Connolly Sent: Tuesday, April 30, 2013 9:54 AM To: k. leigh ad Subject: RE: [Histonet] HIER pressure cookers... Biocare Decloaker here !! Brett M. Connolly, Ph Principal Scientist, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 [9]brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 -----O From: [10]histonet-bounces@lists.utsouthwestern.edu [[11]mailto:histonet-bounc k. leigh adams Sent: Tu To: [12]histonet@lists.utsouthwestern.edu Subject: [His Any input as to preferred ins appreciated... Leigh _________ Histonet mailing list [13]Histonet@lists.utsouthwe [14]http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contai ns information of Merck & Co., Inc. (One Merck Drive, Whitehouse Statio contact information [15]http://www.merck.com/contact/contacts.html) that may confidential, proprietary copyrighted and/or legally privileged. It is i this messag received this message in e-mail and then delete it fro ________________________________________ Histonet mailing list [16]Histonet@lists.utsouthwestern.edu [17]http://lists.uts ________________ Histonet mailing list [18]Histonet@lists.utsouthwestern [19]http://lists.utsouthwestern.edu/mailman/listinfo/histonet References 1. 3D"mailto:lbla 2. 3D"mailto:k.leigh.adams.865@gmail.com" 3. 3D"mailto:histonet@lists.utsout 4. file://localhost/tmp/3D"m 5. 3D"mailto:lblazek 6. 3D"mailto:h 7. 3D"mailto:histonet-bounces@lists.utsouthwestern.edu" 8. 3D"mailto:histonet@lists.utsouthwestern.edu" 9. 3D"mailto:bre 10. 3D"mailto:histonet-bounces@lists.u 11. ="mailto:histonet-bounces@lists.utsouthwestern.edu" 12. 3D"mailto:histonet@lists.ut 13. 3D"mailto:Histonet@lists.utsouthwestern.edu" 14. 3D"http://lists.utsouthwestern.edu/mailman/list 15. 3D"http://www.merck.com/conta 16. 3D"mailto:Histonet@lists. 17. file://localhost/tmp/3D" 18. ="mailto:Histonet@lists.utsouthwestern.edu" 19. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/ ------------------------------ Message: 11 Date: Tue, 30 Apr 2013 17:15:01 +0200 From: "Gudrun Lang" Subject: AW: [Histonet] BIG PROBLEM To: "'Behnaz Sohrab'" Cc: histonet@lists.utsouthwestern.edu Message-ID: <000301ce45b5$7d292a50$777b7ef0$@gmx.at> Content-Type: text/plain; charset="iso-8859-1" Too much blue is mostly a problem of too less red. Have you checked your Eosin? pH? Rinsing after eosin? My personal issue was sometimes too thin sections. Those looked also rather pale. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Behnaz Sohrab Gesendet: Montag, 29. April 2013 19:36 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] BIG PROBLEM Good Morning, We have two Leica H&E stainer( old xt-5010 and new 5020). We do use : Gill's 3 for 6 minutes, 4% Glacial ACETIC Acid 40 seconds Bluing : 45 sec. We done this for years!! last few months our Pantologists are complaining about GI Biopsies that they have TOO much blue in cytoplasma and the Mucin is too purple and blue? Please any suggestion?? Mean while we are trying the NEW kit from Leica for H&E , they think that is TOO pale?any of you has tried this new stain from Lecia? With much application. Behnaz Sohrab, Los Angeles ------------------------------ Message: 12 Date: Tue, 30 Apr 2013 15:30:33 +0000 From: "Lake, Kim S" Subject: [Histonet] Processor question - alcoholic formalin vs. buffered formalin To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" I attended the Tri-State Symposium in Dubuque, IA last week (which was fantastic) and sat in on a "Boot Camp for Histology" workshop. At this workshop I realized that some problems we have been having with sectioning could be due to our processor set up. Before microtomy we have to soak our blocks for 10-15 minutes, and I suspect that this is because we are over dehydrating our tissues in the during processing. Our first solution, which the cassettes sit in for about 8 hours until the overnight processing begins, is alcoholic formalin, which is 450ml buffered formalin, 900ml water, and 3300ml 95% alcohol. Our processor is a Leica TP 1050 that has been chugging along for about 20 years now. The reason we use alcoholic formalin instead of just normal buffered formalin has been lost to the mists of time, and I was wondering what you all use as a holding solution on your processor, and why. Thanks! Kim Lake Laboratory Manager Oral Pathology Laboratory University of Iowa College of Dentistry Phone (319) 384 4433 Fax (319) 353 5569 ------------------------------ Message: 13 Date: Tue, 30 Apr 2013 11:40:53 -0400 From: "Hannen, Valerie" Subject: RE: [Histonet] Processor question - alcoholic formalin vs. buffered formalin To: "'Lake, Kim S'" , "histonet@lists.utsouthwestern.edu" Message-ID: <450B7A81EDA0C54E97C53D60F00776C3231B3D7C33@isexstore03> Content-Type: text/plain; charset="us-ascii" We use 10 % Neutral Buffered Formalin. It is a wonderful fixative and gives us really great fixation. It does sound like you are overly dehydrating your tissues. Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.hannen@parrishmed.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lake, Kim S Sent: Tuesday, April 30, 2013 11:31 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Processor question - alcoholic formalin vs. buffered formalin I attended the Tri-State Symposium in Dubuque, IA last week (which was fantastic) and sat in on a "Boot Camp for Histology" workshop. At this workshop I realized that some problems we have been having with sectioning could be due to our processor set up. Before microtomy we have to soak our blocks for 10-15 minutes, and I suspect that this is because we are over dehydrating our tissues in the during processing. Our first solution, which the cassettes sit in for about 8 hours until the overnight processing begins, is alcoholic formalin, which is 450ml buffered formalin, 900ml water, and 3300ml 95% alcohol. Our processor is a Leica TP 1050 that has been chugging along for about 20 years now. The reason we use alcoholic formalin instead of just normal buffered formalin has been lost to the mists of time, and I was wondering what you all use as a holding solution on your processor, and why. Thanks! Kim Lake Laboratory Manager Oral Pathology Laboratory University of Iowa College of Dentistry Phone (319) 384 4433 Fax (319) 353 5569 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet =================== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" =================== ------------------------------ Message: 14 Date: Tue, 30 Apr 2013 16:31:49 +0000 From: "Margiotta-Watz, Michele" Subject: [Histonet] cassette labelers To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <230D0B9EC57D7A45A7A186C6AB4C7ABC296F39AA@BMH-EXCHANGE-02.BMHMC.ORG> Content-Type: text/plain; charset="us-ascii" Hi All, We are looking to purchase 2 cassette labelers and would like some feedback on which ones you might recommend. Also, is there a difference between Thermo's Printmate and Microwriter? Looks like the Printmate is just a newer version. Your input would be greatly appreciated. Thanks, Michele Histology Supervisor BMHMC 631-654-7192 DISCLAIMER: This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 113, Issue 30 ***************************************** _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Commonwealth Health. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. From plucas <@t> biopath.org Wed May 1 14:26:13 2013 From: plucas <@t> biopath.org (Paula Lucas) Date: Wed May 1 14:16:49 2013 Subject: [Histonet] recycled chemical testing Message-ID: Hello, Could anyone give me a contact where I can send my recycled alcohol and xylene for quality assessment? Thank you, Paula Lucas Lab Manager Bio-Path Medical Group Fountain Valley, CA 92708 From amber.mckenzie <@t> gastrodocs.net Wed May 1 14:32:56 2013 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Wed May 1 14:33:22 2013 Subject: [Histonet] EPA checklist In-Reply-To: <761E2B5697F795489C8710BCC72141FF04CF19@ex07.net.ucsf.edu> References: <1046889651.1684067.1356725919030.JavaMail.root@sz0094a.emeryville.ca.mail.comcast.net> <5A33C952BB67F4468AF1F36D739212BC7B5D2323@JERRY.Gia.com> <761E2B5697F795489C8710BCC72141FF04CF19@ex07.net.ucsf.edu> Message-ID: <5A33C952BB67F4468AF1F36D739212BCBCED1E3A@JERRY.Gia.com> Does anyone have a checklist for EPA? I had 2 representatives stop by unannounced and cite us for several things. Thanks! From amber.mckenzie <@t> gastrodocs.net Wed May 1 14:34:22 2013 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Wed May 1 14:34:25 2013 Subject: [Histonet] EPA Check list In-Reply-To: <450B7A81EDA0C54E97C53D60F00776C3231B3D7C15@isexstore03> References: <450B7A81EDA0C54E97C53D60F00776C3231B3D7C15@isexstore03> Message-ID: <5A33C952BB67F4468AF1F36D739212BCBCED1E47@JERRY.Gia.com> Does anyone have a checklist for EPA? I had 2 representatives stop by unannounced and cite us for several things. Thanks! From kmerriam2003 <@t> yahoo.com Thu May 2 10:34:41 2013 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Thu May 2 10:34:44 2013 Subject: [Histonet] alternates to cryojane Message-ID: <1367508881.1376.YahooMailNeo@web121906.mail.ne1.yahoo.com> Hello eveyone, Are there any other tape transfer systems for the cryostat other than the cryojane? Thanks, Kim ? Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From ryaskovich <@t> dir.nidcr.nih.gov Thu May 2 14:52:18 2013 From: ryaskovich <@t> dir.nidcr.nih.gov (Yaskovich, Ruth A (NIH/NIDCR) [E]) Date: Thu May 2 14:53:50 2013 Subject: [Histonet] Cresyl echt Violett for Nissl substance Message-ID: <502052F0C384624FA5F2EF37D686AACC0F3C49BA@MLBXV06.nih.gov> What is everyone using for this stain? Ruth Yaskovich N.I.H. From brannon <@t> alliedsearchpartners.com Thu May 2 16:04:27 2013 From: brannon <@t> alliedsearchpartners.com (Brannon Owens) Date: Thu May 2 16:04:35 2013 Subject: [Histonet] HT/HTL opening 11pm-7am in Spokane, WA Message-ID: Position: Histotechnician/Histotechnologist Schedule: Monday-Friday 11pm-7am shift Location: Spokane, WA HT or HTL ASCP certified Immunohistochemistry (IHC) experience 4+ years laboratory experience Email Brannon@alliedsearchpartners.com for a full job description! From CDavis <@t> che-east.org Fri May 3 09:02:10 2013 From: CDavis <@t> che-east.org (Davis, Cassie) Date: Fri May 3 09:08:10 2013 Subject: [Histonet] EPA checklist In-Reply-To: References: Message-ID: <08861B9CF6C7774E874635A4818AE37B07A3C6E5@CHEXCMS01.one.ads.che.org> RE "Does anyone have a checklist for EPA? I had 2 representatives stop by unannounced and cite us for several things. Thanks!" If you get one PLEASE forward a copy to me! Cassandra Davis Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From relia1 <@t> earthlink.net Fri May 3 09:26:32 2013 From: relia1 <@t> earthlink.net (Pam Barker) Date: Fri May 3 09:26:38 2013 Subject: [Histonet] RELIA Hot Histology Job Alert!! TGIF!! HT/HTL Busy growing lab Charlotte, NC 10K relo and sign on bonus!! Message-ID: <00f801ce480a$35e891b0$a1b9b510$@earthlink.net> Hi Histonetters!! TGIF Have a great weekend. If you have a second please take a look at this exciting opportunity! HT/HTL Busy growing lab Charlotte,NC 10K relo and sign on bonus!! RELIA Solutions the nation's only recruiting firm specializing in the permanent placement of histology professionals is working with an expanding lab in Charlotte in need of several histotechs. These are full time permanent positions and my client offers excellent compensation great benefits and a 10K sign on/relo bonus. ASCP HT or HTL certification and a minimum of 2 years of histology experience is required. For more information please contact Pam Barker at relia1@earthlink.net or toll free at 866-607-3542 RELIA Solutions is the nation's ONLY recruiting firm specializing in the nationwide permanent placement of histology professionals. To sign up for our free histology careers bulletin please send an e-mail to relia1@earthlink.net and include subscribe in the subject line. Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From mtitford <@t> aol.com Fri May 3 10:37:08 2013 From: mtitford <@t> aol.com (mtitford@aol.com) Date: Fri May 3 10:37:11 2013 Subject: [Histonet] Staining Nissl Message-ID: <8D0162B34AE4287-2198-2CDD2@webmail-d253.sysops.aol.com> Ruth Yaskovich asks about staining Nissl: Years ago, when I started in histology and there were still dinosaurs on earth, we used cresyl fast violet for staining Nissl in celloidin sections, using a fancy differentiation with cresote in alcohol, or something. In paraffin sections you can get pretty good results staining in heated 1% toluidine blue or 0.5% thionin and differentiating in 96% alcohol. There may be some IHC antibody you can use Michael Titford USA Pathology Mobilew AL USA From Ronald.Houston <@t> nationwidechildrens.org Fri May 3 12:22:38 2013 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Fri May 3 12:22:43 2013 Subject: [Histonet] patient release Message-ID: Would someone be willing to share a copy of their pathology slide patient release documentation, and if they have an inclusion acknowledging acceptance that they are responsible for any charges when the patient/family requests the second opinion? Thanks Ronnie Houston From pzeitlow <@t> bbpllab.com Fri May 3 13:36:15 2013 From: pzeitlow <@t> bbpllab.com (Pat Zeitlow) Date: Fri May 3 13:36:21 2013 Subject: [Histonet] BRAF IHC Message-ID: <6DB7235FE14F1E49ABC5794DF98C751F0A679D0D93@exsrv07> Is anyone doing BRAF IHC staining that is willing to share protocol details? TIA, Pat From pzeitlow <@t> bbpllab.com Fri May 3 13:52:49 2013 From: pzeitlow <@t> bbpllab.com (Pat Zeitlow) Date: Fri May 3 13:52:49 2013 Subject: [Histonet] RE: BRAF IHC In-Reply-To: <77F52EFAB8B1694B885E277C48FCD0F63AB79064@GHSEXMBX4W8K1V.geisinger.edu> References: <6DB7235FE14F1E49ABC5794DF98C751F0A679D0D93@exsrv07> <77F52EFAB8B1694B885E277C48FCD0F63AB79064@GHSEXMBX4W8K1V.geisinger.edu> Message-ID: <6DB7235FE14F1E49ABC5794DF98C751F0A679D0DA6@exsrv07> Also using Spring Bioscience antibody. Trying to get it automated using Biocare instrument. You? Pat -----Original Message----- From: Bitting, Angela K. [mailto:akbitting@geisinger.edu] Sent: Friday, May 03, 2013 1:45 PM To: Pat Zeitlow Subject: RE: BRAF IHC Working it up right now. Using Spring Bioscience's BRAF V600E. Are you using an automated platform? Angie -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pat Zeitlow Sent: Friday, May 03, 2013 2:36 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] BRAF IHC Is anyone doing BRAF IHC staining that is willing to share protocol details? TIA, Pat _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. 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From cforster <@t> umn.edu Fri May 3 14:09:00 2013 From: cforster <@t> umn.edu (Colleen Forster) Date: Fri May 3 14:09:15 2013 Subject: [Histonet] BrDU Message-ID: <51840B4C.1080301@umn.edu> What are you guys using as your primary for BrDU these days? Thanks! Colleen Forster U of MN From cforster <@t> umn.edu Fri May 3 14:51:02 2013 From: cforster <@t> umn.edu (Colleen Forster) Date: Fri May 3 14:51:06 2013 Subject: [Histonet] pros and cons of Zinc formalin Message-ID: <51841526.9070802@umn.edu> All information from those who have or are using welcome! Thanks! Colleen Forster U of MN From ccrowder <@t> vetmed.lsu.edu Sat May 4 08:07:24 2013 From: ccrowder <@t> vetmed.lsu.edu (Cheryl Crowder) Date: Sat May 4 08:07:55 2013 Subject: [Histonet] Cresyl echt violet Message-ID: Some years ago the name of cresyl echt violet was changed to cresyl violet acetate (no CI #). It is what is now used in staining for Nissl substance. Cheryl Crowder From susanbachus <@t> verizon.net Sat May 4 08:37:07 2013 From: susanbachus <@t> verizon.net (susanbachus@verizon.net) Date: Sat May 4 08:37:15 2013 Subject: [Histonet] Cresyl echt violet In-Reply-To: References: Message-ID: <8D8F96CA6E284594B4C37A1B6F4A96EA@UserPC> It's important to bear in mind that cresyl violet acetate is not exactly the same formulation as cresyl violet (the pH is different), so it can't be simply substituted in an old protocol in place of cresyl violet--so make sure you work from a protocol specific for cresyl violet acetate. But yes, the results should then be pretty much the same. Here is some fascinating history that I received years ago from Richard W. Dapson at Anatech Ltd. when he kindly donated some old stains to our lab: "Your request is not at all inappropriate! However, you sure picked a tough one. The chemical identity of cresyl violets sold between 1918 and the late 1900's is a mystery. The dye was originally manufactured in Germany for biological use; it was never a textile dye, so supplies were always very limited. The name was cresyl echt violet (echt meaning fast in German). Because of the two World Wars during which dye shipments from Germany were banned, other material appeared on the market under various labels: cresyl echt violet, cresyl fast violet, and cresyl violet, often with letter suffixes like V or R. Most were not identical to the original German material. In 1952, cresyl violet acetate was synthesized in the US to replace all of these other dyes. Unlike the chloride salts which were poorly soluble in anything, the acetate is water soluble and easier to use. Studies have shown it to be a satisfactory replacement for the original dye, at least for staining Nissl substance. The problem is what dye is really inside any given bottle, especially those packaged before about 1980. If you want true cresyl violet acetate, buy it from Aldrich. That is the real stuff as labeled, but it is not cresyl echt violet." -----Original Message----- From: Cheryl Crowder Sent: Saturday, May 04, 2013 9:07 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cresyl echt violet Some years ago the name of cresyl echt violet was changed to cresyl violet acetate (no CI #). It is what is now used in staining for Nissl substance. Cheryl Crowder _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akemiat3377 <@t> yahoo.com Sun May 5 18:05:57 2013 From: akemiat3377 <@t> yahoo.com (Akemi Allison) Date: Sun May 5 18:06:03 2013 Subject: [Histonet] Happy 79th Birthday Jules Elias Message-ID: <1367795157.85164.YahooMailNeo@web140604.mail.bf1.yahoo.com> For those of you who were wondering what happened to Jules Elias. It's his 79th Birthday today! I just got an email from Jules telling me he and Renee just recently returned from a month long cruise to Tahiti and Hawaii. They still do a fair job on the dance floor. His music making has reached higher levels than he ever anticipated and his time is quite taken up by rehearsals and performances. He's thinking of giving a NSH seminar on the history of our profession starting with the days at the AFIP with Lee and Nick and going to where we are now as a recognized profession by both pathologists and other medical professionals. ? Akemi Allison, BS.,HT/HTL (ASCP) From xylene02 <@t> yahoo.com Sun May 5 18:25:15 2013 From: xylene02 <@t> yahoo.com (jesse andrade) Date: Sun May 5 18:25:19 2013 Subject: [Histonet] Holzer method for glial fibers Message-ID: <1367796315.78613.YahooMailNeo@web120406.mail.ne1.yahoo.com> I have a question about this method and the purpose of the phosphomolybdic acid-alcohol solution in this stain? I am astudent and need this for my staining procedure assignment. From tony.henwood <@t> health.nsw.gov.au Sun May 5 18:37:55 2013 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Sun May 5 18:38:22 2013 Subject: [Histonet] Happy 79th Birthday Jules Elias In-Reply-To: <1367795157.85164.YahooMailNeo@web140604.mail.bf1.yahoo.com> References: <1367795157.85164.YahooMailNeo@web140604.mail.bf1.yahoo.com> Message-ID: <6D6BD1DE8A5571489398B392A38A71579D246BDD@xmdb04.nch.kids> Well done Jules. Jules visited us "down-under" a few years ago as a guest lecturer at our Scientific Meeting and what an entertaining and knowledgeable presenter he was. It is great to know he is still kicking-up his heels. A great leader of our profession. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison Sent: Monday, 6 May 2013 9:06 AM To: Histonet Subject: [Histonet] Happy 79th Birthday Jules Elias For those of you who were wondering what happened to Jules Elias. It's his 79th Birthday today! I just got an email from Jules telling me he and Renee just recently returned from a month long cruise to Tahiti and Hawaii. They still do a fair job on the dance floor. His music making has reached higher levels than he ever anticipated and his time is quite taken up by rehearsals and performances. He's thinking of giving a NSH seminar on the history of our profession starting with the days at the AFIP with Lee and Nick and going to where we are now as a recognized profession by both pathologists and other medical professionals. ? Akemi Allison, BS.,HT/HTL (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From MMargiotta <@t> bmhmc.org Mon May 6 06:35:42 2013 From: MMargiotta <@t> bmhmc.org (Margiotta-Watz, Michele) Date: Mon May 6 06:35:48 2013 Subject: [Histonet] Happy 79th Birthday Jules Elias In-Reply-To: <1367795157.85164.YahooMailNeo@web140604.mail.bf1.yahoo.com> References: <1367795157.85164.YahooMailNeo@web140604.mail.bf1.yahoo.com> Message-ID: <230D0B9EC57D7A45A7A186C6AB4C7ABC296F4DF2@BMH-EXCHANGE-02.BMHMC.ORG> Happy,happy Birthday Jules! I started my Histo career working with Jules @ Stony Brook and he was an inspiration to me! I was very lucky to have learned a lot about this profession from him. Wishing you a great day!! Michele Margiotta-Watz Histology Supervisor BMHMC -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison Sent: Sunday, May 05, 2013 7:06 PM To: Histonet Subject: [Histonet] Happy 79th Birthday Jules Elias For those of you who were wondering what happened to Jules Elias. It's his 79th Birthday today! I just got an email from Jules telling me he and Renee just recently returned from a month long cruise to Tahiti and Hawaii. They still do a fair job on the dance floor. His music making has reached higher levels than he ever anticipated and his time is quite taken up by rehearsals and performances. He's thinking of giving a NSH seminar on the history of our profession starting with the days at the AFIP with Lee and Nick and going to where we are now as a recognized profession by both pathologists and other medical professionals. ? Akemi Allison, BS.,HT/HTL (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet DISCLAIMER: This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. From DKnutson <@t> primecare.org Mon May 6 07:12:57 2013 From: DKnutson <@t> primecare.org (Knutson, Deanne) Date: Mon May 6 07:13:05 2013 Subject: [Histonet] CAP standard GEN 61300 Message-ID: <1E0E2B14C709174B8AC2BE0AE7F76833A2EFE4AB1C@EXCHANGE2K7.staprimecare.org> Fellow Histonetters, I am looking for advice concerning CAP standard GEN 61300 Climate Control. To those that take humidity records in your lab, what exact % range do you use for a normal range? And what do you do if and when you are out of this range? Thank you for sharing your workflow info with me. Deanne Knutson Anatomic Pathology Supervisor St. Alexius Medical Center 701-530-6730 dknutson@primecare.org ________________________________ This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. From BJohnson <@t> pbmlabs.com Mon May 6 08:45:11 2013 From: BJohnson <@t> pbmlabs.com (Brian Johnson) Date: Mon May 6 08:45:18 2013 Subject: [Histonet] Histotech Position Message-ID: PBM Labs, LLP is seeking a Histology Technician or Histology Technologist to work nights. The shift can either be 1:00am - 9:30am or 2:00am - 10:30am, depending on the candidate's preference. The ideal candidate will possess a high school diploma or equivalent, with a demonstrated ability to perform tasks with a high degree of accuracy/quality. At least one year of previous Histology Tech experience and National HT (ASCP) certification preferred. Duties will include, but are not limited to: ? Perform assigned pre and post analytical activities related to anatomic pathology tests assigned including specimen collection and processing, Quality Control, equipment maintenance, inventory control, Quality Assurance and proficiency testing procedures ? Perform analytical activities related to specimen processing, embedding, cutting, routine and special histologic staining, frozen sectioning, coverslipping, and handling of irreplaceable tissue, surgical, and body fluid specimens Interested candidates should contact Human Resources at 972-966-7807. NO RECRUITERS OR AGENCIES, PLEASE. PBM Labs is an Equal Opportunity Employer. PBM Labs PRIVACY AND CONFIDENTIALITY NOTICE: The information transmitted in this message and any attachments is intended for the person or entity to which it is addressed and may contain proprietary, confidential, and/or privileged material, which may include individually identifiable health information that requires safeguarding in compliance with the Health Insurance Portability and Accountability Act of 1996 as well as other federal and state laws. Any review, duplication, retransmission, distribution, or other use of, or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you have received this message in error, please notify the sender immediately by return e-mail and delete all of the information from all computers or systems. From tahseen <@t> brain.net.pk Mon May 6 08:47:46 2013 From: tahseen <@t> brain.net.pk (tahseen) Date: Mon May 6 08:47:56 2013 Subject: [Histonet] CD 24 CLONE 528807 and CD 44s Pan Message-ID: Hi All I need help who are working with CD 24 CLONE 528807 Catalog Number MAB 5248 (R&D Systems) and CD 44s Pan Clone 2cs Catalog Number BBA10 on formalin fixed paraffin embedded sections. 1 Reconstitute at .5ml OR 1 ml with PBS OR DW? 2 What is the dilution factor? 3 Reconstituted & diluted antibody storage temp? 4 Good control tissue? Any other finger tips. Thanks advance. Muhammad Tahseen Senior supervisor Histopathology SKMCH&RC Lahore Pakistan From mcauliff <@t> umdnj.edu Mon May 6 09:19:33 2013 From: mcauliff <@t> umdnj.edu (Geoff) Date: Mon May 6 09:19:38 2013 Subject: [Histonet] 5 gal specimen container search In-Reply-To: <9BF995BC0E47744E9673A41486E24EE2579B14884C@MERCERMAIL.MercerU.local> References: <8D00F3AD149D8FB-1314-B9E6@webmail-m173.sysops.aol.com> <450B7A81EDA0C54E97C53D60F00776C3231B3D7C30@isexstore03> <9BF995BC0E47744E9673A41486E24EE2579B14884C@MERCERMAIL.MercerU.local> Message-ID: <5187BBF5.20900@umdnj.edu> Lowe's or Home Depot. They don't say "science" on the side but who cares? Geoff On 4/30/2013 10:38 AM, Shirley A. Powell wrote: > You can also check with restaurant supply houses, we have purchased great large containers with lids for this purpose. Much cheaper than the lab supply houses. > Shirley > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie > Sent: Tuesday, April 30, 2013 10:36 AM > To: 'Jackie O'Connor'; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] 5 gal specimen container search > > Jackie... > > We have ordered these from a company by the name of Centurian Medical. The lids are sold separately. > > Valerie > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie O'Connor > Sent: Wednesday, April 24, 2013 3:41 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] 5 gal specimen container search > > > Anyone in histoland know a vendor where I can find a 5 gallon leakproof bucket, drum, pail for shipping a large pathology specimen? > > Jackie O' > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ============= > "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" > ============= > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From TMcNemar <@t> lmhealth.org Mon May 6 09:48:19 2013 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Mon May 6 09:47:15 2013 Subject: [Histonet] RE: CAP standard GEN 61300 In-Reply-To: <1E0E2B14C709174B8AC2BE0AE7F76833A2EFE4AB1C@EXCHANGE2K7.staprimecare.org> References: <1E0E2B14C709174B8AC2BE0AE7F76833A2EFE4AB1C@EXCHANGE2K7.staprimecare.org> Message-ID: I am very interested in this as well. We have an ongoing issue with humidity/static. I have been unable to find anything that lists a recommended humidity level for histology. New construction building codes for labs list 30% - 80% and the equipment manufacturers list 30% - 80% for their recommended operating environment. Based on two different digital readers, our humidity is sometimes in the 20% range (mostly in winter but not always). A hand operated device reads about 40% higher than the digitals so I don't quite know what to make of it. Anyway, I will appreciated any input. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Knutson, Deanne Sent: Monday, May 06, 2013 8:13 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] CAP standard GEN 61300 Fellow Histonetters, I am looking for advice concerning CAP standard GEN 61300 Climate Control. To those that take humidity records in your lab, what exact % range do you use for a normal range? And what do you do if and when you are out of this range? Thank you for sharing your workflow info with me. Deanne Knutson Anatomic Pathology Supervisor St. Alexius Medical Center 701-530-6730 dknutson@primecare.org ________________________________ This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From katelin09htl <@t> gmail.com Mon May 6 10:12:01 2013 From: katelin09htl <@t> gmail.com (Katelin Lester) Date: Mon May 6 10:12:06 2013 Subject: [Histonet] RE: CAP standard GEN 61300 In-Reply-To: References: <1E0E2B14C709174B8AC2BE0AE7F76833A2EFE4AB1C@EXCHANGE2K7.staprimecare.org> Message-ID: We are also going through humidity issues post inspection. Our values are based on the specifications in the equipment manuals and are 30%-80%. We use a digital reader. We have been dipping below that and are working to get the humidity up. We have brought in a small humidifier but it is not keeping up on very dry days. It is in our policy to report out of range values to our supervisor and she is now looking in to a more permanent solution. We would also appreciate any comments on how other labs have increased their humidity. Katelin Lester, HTL On May 6, 2013 7:47 AM, "Tom McNemar" wrote: > I am very interested in this as well. We have an ongoing issue with > humidity/static. I have been unable to find anything that lists a > recommended humidity level for histology. New construction building codes > for labs list 30% - 80% and the equipment manufacturers list 30% - 80% for > their recommended operating environment. > > Based on two different digital readers, our humidity is sometimes in the > 20% range (mostly in winter but not always). A hand operated device reads > about 40% higher than the digitals so I don't quite know what to make of it. > > Anyway, I will appreciated any input. > > Tom McNemar, HT(ASCP) > Histology Co-ordinator > Licking Memorial Health Systems > (740) 348-4163 > (740) 348-4166 > tmcnemar@lmhealth.org > www.LMHealth.org > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Knutson, Deanne > Sent: Monday, May 06, 2013 8:13 AM > To: 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] CAP standard GEN 61300 > > Fellow Histonetters, > > I am looking for advice concerning CAP standard GEN 61300 Climate Control. > > To those that take humidity records in your lab, what exact % range do you > use for a normal range? > > And what do you do if and when you are out of this range? > > Thank you for sharing your workflow info with me. > > Deanne Knutson > Anatomic Pathology Supervisor > St. Alexius Medical Center > 701-530-6730 > dknutson@primecare.org > > > > ________________________________ > This email may include confidential and privileged information. If this is > not intended for your use, please destroy immediately and contact the > sender of the message. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This e-mail, including attachments, is intended for the sole use of the > individual and/or entity to whom it is addressed, and contains information > from Licking Memorial Health Systems which is confidential or privileged. > If you are not the intended recipient, nor authorized to receive for the > intended recipient, be aware that any disclosure, copying, distribution or > use of the contents of this e-mail and attachments is prohibited. If you > have received this in error, please advise the sender by reply e-mail and > delete the message immediately. You may also contact the LMH Process > Improvement Center at 740-348-4641. E-mail transmissions cannot be > guaranteed to be secure or error-free as information could be intercepted, > corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. > The sender therefore does not accept liability for any errors or omissions > in the contents of this message, which arise as a result of e-mail > transmission. Thank you. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From brett_connolly <@t> merck.com Mon May 6 10:19:09 2013 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Mon May 6 10:19:18 2013 Subject: [Histonet] Adenosine 2A receptor IHC Message-ID: Hi All, Looking for antibodies for the A2A receptor for FFPE tissues - any suggestions would be appreciated. Thanks, Brett Brett M. Connolly, Ph.D. Principal Scientist, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From wdesalvo.cac <@t> outlook.com Mon May 6 10:35:04 2013 From: wdesalvo.cac <@t> outlook.com (WILLIAM DESALVO) Date: Mon May 6 10:35:09 2013 Subject: [Histonet] RE: CAP standard GEN 61300 In-Reply-To: References: <1E0E2B14C709174B8AC2BE0AE7F76833A2EFE4AB1C@EXCHANGE2K7.staprimecare.org>, , Message-ID: To address Gen 61300, you have to check your instrument manuals, and MSDS for all your reagents. Create the acceptable ranges and then monitor. Make sure you create a process to address out of range issues. We use Tutela Medical devices to monitor both temp and humidity. Our facilites installed and maintains records. http://www.tutelamedical.com/ William DeSalvo, BS HTL(ASCP) Production Manager-Anatomic Pathology Chair, NSH Quality Management Committee Owner/Consultant, Collaborative Advantage Consulting > Date: Mon, 6 May 2013 08:12:01 -0700 > From: katelin09htl@gmail.com > To: TMcNemar@lmhealth.org > Subject: Re: [Histonet] RE: CAP standard GEN 61300 > CC: histonet@lists.utsouthwestern.edu; DKnutson@primecare.org > > We are also going through humidity issues post inspection. Our values are > based on the specifications in the equipment manuals and are 30%-80%. We > use a digital reader. We have been dipping below that and are working to > get the humidity up. We have brought in a small humidifier but it is not > keeping up on very dry days. > It is in our policy to report out of range values to our supervisor and she > is now looking in to a more permanent solution. > We would also appreciate any comments on how other labs have increased > their humidity. > > Katelin Lester, HTL > On May 6, 2013 7:47 AM, "Tom McNemar" wrote: > > > I am very interested in this as well. We have an ongoing issue with > > humidity/static. I have been unable to find anything that lists a > > recommended humidity level for histology. New construction building codes > > for labs list 30% - 80% and the equipment manufacturers list 30% - 80% for > > their recommended operating environment. > > > > Based on two different digital readers, our humidity is sometimes in the > > 20% range (mostly in winter but not always). A hand operated device reads > > about 40% higher than the digitals so I don't quite know what to make of it. > > > > Anyway, I will appreciated any input. > > > > Tom McNemar, HT(ASCP) > > Histology Co-ordinator > > Licking Memorial Health Systems > > (740) 348-4163 > > (740) 348-4166 > > tmcnemar@lmhealth.org > > www.LMHealth.org > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Knutson, Deanne > > Sent: Monday, May 06, 2013 8:13 AM > > To: 'histonet@lists.utsouthwestern.edu' > > Subject: [Histonet] CAP standard GEN 61300 > > > > Fellow Histonetters, > > > > I am looking for advice concerning CAP standard GEN 61300 Climate Control. > > > > To those that take humidity records in your lab, what exact % range do you > > use for a normal range? > > > > And what do you do if and when you are out of this range? > > > > Thank you for sharing your workflow info with me. > > > > Deanne Knutson > > Anatomic Pathology Supervisor > > St. Alexius Medical Center > > 701-530-6730 > > dknutson@primecare.org > > > > > > > > ________________________________ > > This email may include confidential and privileged information. If this is > > not intended for your use, please destroy immediately and contact the > > sender of the message. > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > This e-mail, including attachments, is intended for the sole use of the > > individual and/or entity to whom it is addressed, and contains information > > from Licking Memorial Health Systems which is confidential or privileged. > > If you are not the intended recipient, nor authorized to receive for the > > intended recipient, be aware that any disclosure, copying, distribution or > > use of the contents of this e-mail and attachments is prohibited. If you > > have received this in error, please advise the sender by reply e-mail and > > delete the message immediately. You may also contact the LMH Process > > Improvement Center at 740-348-4641. E-mail transmissions cannot be > > guaranteed to be secure or error-free as information could be intercepted, > > corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. > > The sender therefore does not accept liability for any errors or omissions > > in the contents of this message, which arise as a result of e-mail > > transmission. Thank you. > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lcolbert <@t> pathmdlabs.com Mon May 6 10:36:33 2013 From: lcolbert <@t> pathmdlabs.com (Laurie Colbert) Date: Mon May 6 10:37:19 2013 Subject: [Histonet] Adenovirus controls Message-ID: <12ECD7346266D74691EC2BFC75285E452F2DD7F6@BFL323E10.pathmdlabs.local> Does anyone have a source for purchasing adenovirus control slides? Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab From Timothy.Morken <@t> ucsfmedctr.org Mon May 6 11:16:46 2013 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Mon May 6 11:16:58 2013 Subject: [Histonet] Happy 79th Birthday Jules Elias In-Reply-To: <230D0B9EC57D7A45A7A186C6AB4C7ABC296F4DF2@BMH-EXCHANGE-02.BMHMC.ORG> References: <1367795157.85164.YahooMailNeo@web140604.mail.bf1.yahoo.com> <230D0B9EC57D7A45A7A186C6AB4C7ABC296F4DF2@BMH-EXCHANGE-02.BMHMC.ORG> Message-ID: <761E2B5697F795489C8710BCC72141FF07D78E@ex07.net.ucsf.edu> Happy Birthday to Jules. I took my first in-depth immunohistochemistry course from him - a 2 day seminar. Since then I've read his articles and books extensively and he has been a great help. A true driver in the field. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Margiotta-Watz, Michele Sent: Monday, May 06, 2013 4:36 AM To: 'Akemi Allison'; Histonet Subject: RE: [Histonet] Happy 79th Birthday Jules Elias Happy,happy Birthday Jules! I started my Histo career working with Jules @ Stony Brook and he was an inspiration to me! I was very lucky to have learned a lot about this profession from him. Wishing you a great day!! Michele Margiotta-Watz Histology Supervisor BMHMC -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison Sent: Sunday, May 05, 2013 7:06 PM To: Histonet Subject: [Histonet] Happy 79th Birthday Jules Elias For those of you who were wondering what happened to Jules Elias. It's his 79th Birthday today! I just got an email from Jules telling me he and Renee just recently returned from a month long cruise to Tahiti and Hawaii. They still do a fair job on the dance floor. His music making has reached higher levels than he ever anticipated and his time is quite taken up by rehearsals and performances. He's thinking of giving a NSH seminar on the history of our profession starting with the days at the AFIP with Lee and Nick and going to where we are now as a recognized profession by both pathologists and other medical professionals. ? Akemi Allison, BS.,HT/HTL (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet DISCLAIMER: This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TJohnson <@t> gnf.org Mon May 6 11:48:58 2013 From: TJohnson <@t> gnf.org (Teri Johnson) Date: Mon May 6 11:49:06 2013 Subject: [Histonet] Re: pros and cons of Zinc formalin Message-ID: <9F3CFEE76E51B64991C7485270890B404976BFA4@EX4.lj.gnf.org> Hi Colleen, I have used Zinc formalin in the past, and here are some issues we faced. You must not keep the tissues in Zinc formalin for an extended period of time or they get brittle. You must rinse the zinc formalin fixed tissues with water prior to putting them into any phosphate buffered solution. Otherwise you will get a fine white precipitate that can settle into your tissues. If your pathologists are not accustomed to seeing the level of cellular detail (artifact) seen with this fixative, it becomes an issue for them. They need to know it has been fixed in something other than NBF. Silver stains may not stain as cleanly as NBF fixed tissues. IHC retrieval may need to be altered from your SOPs. Other than that, our hematopathologist loved it for lymph nodes and bone marrows. It became the lab standard after getting rid of B-5 fixative. Teri Johnson Manager, Histology GNF - San Diego, CA 858-332-4752 From abijag76 <@t> rediffmail.com Mon May 6 11:57:09 2013 From: abijag76 <@t> rediffmail.com (abijag ) Date: Mon May 6 11:57:22 2013 Subject: [Histonet] staining of immature collagen in lung fibrosis models Message-ID: <20130506165709.12098.qmail@f4mail-235-233.rediffmail.com> Dear Histonetters, I am looking for hearing from our experienced histonetters regarding the possibility of staining of immature collagen fibres in animal models of lung fibrosis. I didn't find any histochemical method in the literature to stain the immature collagen. I would like to know whether any specific method is available for staining or is there is possible to differentiate them from mature collagen fibres by means of existing methods(for eg. Scirius red or masson's trichrome) Thanks in advance Best regards Abi Jagannath From liz <@t> premierlab.com Mon May 6 12:15:42 2013 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Mon May 6 12:15:44 2013 Subject: [Histonet] staining of immature collagen in lung fibrosis models In-Reply-To: <20130506165709.12098.qmail@f4mail-235-233.rediffmail.com> References: <20130506165709.12098.qmail@f4mail-235-233.rediffmail.com> Message-ID: <14E2C6176416974295479C64A11CB9AE016411AC2C96@SBS2K8.premierlab.local> Abi You can perform a Picro Sirius red and that (based upon the literature) will distinguish between Collagen I/III. You can IHC stain for collagen I and collagen III in mouse, or possibly look at fibroblasts or collagen precursors. We have not done this in lung but we have stained for a wide variety of IHC markers in wound healing models. Good Luck. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Laboratory Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 Work (303) 682-3949 Fax (303) 682-9060 Cell (303) 881-0763 liz@premierlab.com www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of abijag Sent: Monday, May 06, 2013 10:57 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] staining of immature collagen in lung fibrosis models Dear Histonetters, I am looking for hearing from our experienced histonetters regarding the possibility of staining of immature collagen fibres in animal models of lung fibrosis. I didn't find any histochemical method in the literature to stain the immature collagen. I would like to know whether any specific method is available for staining or is there is possible to differentiate them from mature collagen fibres by means of existing methods(for eg. Scirius red or masson's trichrome) Thanks in advance Best regards Abi Jagannath _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Mon May 6 13:01:34 2013 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Mon May 6 13:01:42 2013 Subject: [Histonet] Happy 79th Birthday Jules Elias Message-ID: <9ep957wg9g43nggpwi4opgfh.1367863294658@email.android.com> Me too Tim when was that had to have been late 80'$ or so. Jules was worried about med techs taking over ihc from us so he came to the NSH bod and asked us to develop a certification in ihc for ht's since NSH is not a credentialing body Freida CArson suggested we petition ascp and that is how the QIHC exam came about. Thank you Jules for all u have done look where we are today there is not an AP lab worth mentioning that doesn't do ihc and many are also doing ish. From Bruce_Palmatier <@t> vwr.com Mon May 6 13:57:06 2013 From: Bruce_Palmatier <@t> vwr.com (Bruce_Palmatier@vwr.com) Date: Mon May 6 13:57:21 2013 Subject: [Histonet] AUTO: Bruce Palmatier is out of the office (returning 05/07/2013) Message-ID: I am out of the office until 05/07/2013. I will be out of the office on May 6th. If you have a question about an order or need assistance placing an order, or if the matter is urgent, please call 877-881-1192 between 8am and 8pm and a VWR Healthcare Service associate will assist you. For quote requests and pricing-related inquiries, I will respond with 24 hours. Thank You, Bruce Palmatier VWR Healthcare mobile 484-319-5563 Note: This is an automated response to your message "Histonet Digest, Vol 114, Issue 5" sent on 5/6/2013 1:04:42 PM. This is the only notification you will receive while this person is away. From lins0701 <@t> gmail.com Mon May 6 19:20:26 2013 From: lins0701 <@t> gmail.com (Sophia Lin) Date: Mon May 6 19:20:29 2013 Subject: [Histonet] Per Diem Histotech Message-ID: Per Diem histotech needed in Temecula/Murrieta area to cover for vacation, sick leave, and other arising situations. Must have certification, or be certification eligible. Position on on-call basis, with possible increase in hours. Scheduling is flexible, but is current to 4am-8:30am or 12pm-4pm. Must be proficient in embedding, sectioning, staining, and possible operation of processors and stainers. Only serious inquiries please. Please submit resume, letter and references. Thanks! Sophia From sbaldwin <@t> mhhcc.org Tue May 7 09:33:41 2013 From: sbaldwin <@t> mhhcc.org (Sara Baldwin/mhhcc.org) Date: Tue May 7 09:33:36 2013 Subject: [Histonet] histo special request forms Message-ID: Hi Histonetters Would anyone be willing to share their histo special request forms, we are thinking of using these to keep track of what our pathologists want done some write it down and some just call our histotechs and we are losing track of billing!! So would someone please share? Thanks Histology/Cytology Supervisor S. Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph 812-996-0210, 0216, Fax 812-996-0232, Pager 812-481-0897, Cell 812-887-3357 From vperez <@t> pathreflab.com Tue May 7 10:55:29 2013 From: vperez <@t> pathreflab.com (Vanessa Perez) Date: Tue May 7 10:55:34 2013 Subject: FW: [Histonet] histo special request forms Message-ID: Ours is built into our LIS system. So when the doc places the order also puts the charges in. Vanessa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sara Baldwin/mhhcc.org Sent: Tuesday, May 07, 2013 9:34 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] histo special request forms Hi Histonetters Would anyone be willing to share their histo special request forms, we are thinking of using these to keep track of what our pathologists want done some write it down and some just call our histotechs and we are losing track of billing!! So would someone please share? Thanks Histology/Cytology Supervisor S. Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph 812-996-0210, 0216, Fax 812-996-0232, Pager 812-481-0897, Cell 812-887-3357 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Tue May 7 11:50:39 2013 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Tue May 7 11:50:41 2013 Subject: [Histonet] Colorado Sakura Sales Rep Message-ID: <14E2C6176416974295479C64A11CB9AE016411AC2CBC@SBS2K8.premierlab.local> Does anyone out there have the contact information for the Sakura Rep for Colorado. I have been trying to contact someone regarding an embedding cryo console but have not been successful. I do have an additional question, our cryo console has essentially stopped working, we only purchased it about a year and a half ago and we don't even us it every day, it started having problems several months ago. One of my equipment repair guys told me that he has seen this before. I'm just checking to see if this is a rare event or not. Thanks in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Laboratory Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 Work (303) 682-3949 Fax (303) 682-9060 Cell (303) 881-0763 liz@premierlab.com www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 From rheyna <@t> lumc.edu Tue May 7 12:43:51 2013 From: rheyna <@t> lumc.edu (Roger Heyna) Date: Tue May 7 12:44:06 2013 Subject: [Histonet] Monitoring Histology Quality Message-ID: <5188F707020000230004E798@gwgwia1.luhs.org> Is anyone using an electronic system to communicate histology quality info from the pathologists to Histology management? Also, do you use this same system to demonstrate to the CAP that histology quality is being checked daily, and if so, how do you indicate that slide quality was checked when there are no quality issues on a given day? We operate in Sunquest CoPath. Thank you, Roger Heyna Histology Supervisor Maywood, IL From JEllin <@t> yumaregional.org Tue May 7 12:47:41 2013 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Tue May 7 12:47:47 2013 Subject: [Histonet] Monitoring Histology Quality In-Reply-To: <5188F707020000230004E798@gwgwia1.luhs.org> References: <5188F707020000230004E798@gwgwia1.luhs.org> Message-ID: We use Sunquest PowerPath to electronically show this information as well as keep records for data minning and Quality improvement -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Roger Heyna Sent: Tuesday, May 07, 2013 10:44 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Monitoring Histology Quality Is anyone using an electronic system to communicate histology quality info from the pathologists to Histology management? Also, do you use this same system to demonstrate to the CAP that histology quality is being checked daily, and if so, how do you indicate that slide quality was checked when there are no quality issues on a given day? We operate in Sunquest CoPath. Thank you, Roger Heyna Histology Supervisor Maywood, IL ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ From raestask <@t> grics.net Tue May 7 12:56:08 2013 From: raestask <@t> grics.net (Rae Staskiewicz) Date: Tue May 7 12:56:12 2013 Subject: [Histonet] H pylori and Ventana instruments Message-ID: <20130507135608.jn2udaa9ulssokgk@webmail2.centurytel.net> Hi all, Those of you using Ventana instrumentation, could you tell me what H pylori antibody you are using, and whether or not you are experiencing non-specific staining, or staining that looks specific but with a stippled effect. Thanks Rae Staskiewicz UnityPoint Health-Methodist From mmackinnon <@t> dermatologyconsultants.com Tue May 7 14:30:04 2013 From: mmackinnon <@t> dermatologyconsultants.com (Michelle M. Mackinnon) Date: Tue May 7 14:27:28 2013 Subject: [Histonet] Immunohistochmistry Message-ID: <7141C086431ED24FB7F1E0E66028BC1B040ABD87@mail.derm.local> Has anyone tried or have a Wave RPD system from Celerus Diagnostics in their pathology lab? If yes, do you stain immunohistochemisrty on Mohs and paraffin embedded slides? Would you suggest this instrument for a small lab? Also what are the pros and cons using the Wave RPD system performing paraffin embedded tissue and mohs? dermatology CONSULTANTS skin. care. experts. NOTICE OF CONFIDENTIALITY: The information in this email may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipeint, you are hereby notified that any dissemination, distrubution, or copying of this email is strictly prohibited. If you have received this email in error, please notify us immediately by replying to this email and deleting it from your computer. Thank you Dermatology Consultants, PA Please consider the environment before printing. From jennifer.yearley <@t> merck.com Tue May 7 15:43:51 2013 From: jennifer.yearley <@t> merck.com (Yearley, Jennifer Holmes) Date: Tue May 7 15:44:10 2013 Subject: [Histonet] histotech job opening, Merck Research Laboratories, Palo Alto CA Message-ID: <9994A6F228ED9349B09658B786776CF7B486AA746B@USCTMXP51009.merck.com> Histotechnician/Histotechnologist - PHA001165 https://merck.taleo.net/careersection/merck_external_career_section/jobdetail.ftl?job=575074&lang=en Merck is a global health care leader with a diversified portfolio of prescription medicines, vaccines and consumer health products, as well as animal health products. Today, we are building a new kind of healthcare company - one that is ready to help create a healthier future for all of us. Our ability to excel depends on the integrity, knowledge, imagination, skill, diversity and teamwork of people like you. To this end, we strive to create an environment of mutual respect, encouragement and teamwork. As part of our global team, you'll have the opportunity to collaborate with talented and dedicated colleagues while developing and expanding your career. The Pathology Department at Merck Research Laboratories, Palo Alto, is currently seeking an energetic, self-motivated, highly collaborative histotechnician/histotechnologist to join our histology group to help support basic research, mechanistic studies, and biomarker development for evaluations of candidate biological pharmaceuticals. The successful candidate will have a strong histology background, excellent organizational skills and be a confident multitasker, able to juggle multiple responsibilities in a team environment, often under stringent time pressures. Responsibilities for the position will include: * Assistance with mouse tissue collection/tissue handling at necropsy * Familiarity with and implementation of routine fixation and decalcification protocols * Grossing in of a wide array of human and animal tissues, both osseous and non-osseous * Assistance with data entry and database updating for specimens in in-house tissue bank * Fixed tissue processing and maintenance of tissue processors * Tissue embedding into paraffin/OCT * Sectioning of FFPE and frozen tissue blocks * Labeling of slides and tissue cassettes using automated slide and cassette printers * Performance of routine (H&E) and special histochemical stains (both manual and automated) The succesful candidate will be a strong communicator with good writing skills, which will be used to generate well-organized, comprehensive, and timely documentation of work planned and results on completion. The successful candidate will occasionally be responsible, with supervision, for designing small experiments to test new methods, improve old methods, and/or answer well-defined questions pertaining to tissue properties. The successful candidate will consult with investigators on points of technical detail regarding tissue handling as needed. Comfort with common office computer software (e.g. Microsoft Office) is essential. Education: * BA/BS in the biological sciences, with a minimum of 3 years full-time work in a biological research or clinical laboratory or a MS degree in Biological Sciences Preferred Skills and Experience: * HT(ASCP) or HTL(ASCP) certification * Familiarity with principles and techniques of immunohistochemistry Our employees are the key to our company's success. We demonstrate our commitment to our employees by offering a competitive and valuable rewards program. Merck's benefits are designed to support the wide range of goals, needs and lifestyles of our employees, and many of the people that matter the most in their lives. Search Firm Representatives Please Read Carefully: Merck is not accepting unsolicited assistance from search firms for this employment opportunity. Please, no phone calls or emails. All resumes submitted by search firms to any employee at Merck via-email, the Internet or in any form and/or method without a valid written search agreement in place for this position will be deemed the sole property of Merck. No fee will be paid in the event the candidate is hired by Merck as a result of the referral or through other means. Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From azdudley <@t> hotmail.com Tue May 7 16:20:50 2013 From: azdudley <@t> hotmail.com (anita dudley) Date: Tue May 7 16:20:53 2013 Subject: [Histonet] cap anp.23120 Message-ID: Wondering what people are doing about the new cap anp.23120? validating tissue processing schedules. Thanks a lot Anita Dudley Providence Hospital Mobile, Alabama From LSebree <@t> uwhealth.org Tue May 7 16:25:54 2013 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Tue May 7 16:25:58 2013 Subject: [Histonet] H pylori and Ventana instruments In-Reply-To: <20130507135608.jn2udaa9ulssokgk@webmail2.centurytel.net> References: <20130507135608.jn2udaa9ulssokgk@webmail2.centurytel.net> Message-ID: <77DD817201982748BC67D7960F2F76AF048AB3@UWHC-MBX12.uwhis.hosp.wisc.edu> Rae, We use Ventana's HP antibody with no problems. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rae Staskiewicz Sent: Tuesday, May 07, 2013 12:56 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] H pylori and Ventana instruments Hi all, Those of you using Ventana instrumentation, could you tell me what H pylori antibody you are using, and whether or not you are experiencing non-specific staining, or staining that looks specific but with a stippled effect. Thanks Rae Staskiewicz UnityPoint Health-Methodist _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpwenk <@t> sbcglobal.net Tue May 7 19:02:32 2013 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Tue May 7 19:02:33 2013 Subject: [Histonet] Cresyl echt Violett for Nissl substance In-Reply-To: <502052F0C384624FA5F2EF37D686AACC0F3C49BA@MLBXV06.nih.gov> References: <502052F0C384624FA5F2EF37D686AACC0F3C49BA@MLBXV06.nih.gov> Message-ID: <43E80CC7248D41719FAAB4BC0CB956D9@HP2010> Cresyl Echt Violet (CEV) is the pre-WWI name. After factories in Germany were bombed, the formulation for this stain was lost (that's the story I heard in the 70's). Closest companies since then have made is cresyl violet acetate (CVA), though for many decades, continued to call it CEV. Only recently (maybe last 10 years) have companies been calling it CVA, which is confusing to labs that need to order a 25 gram bottle once every 25 years. Here's how we make it up: CRESYL ECHT VIOLET Cresyl echt violet (Cresyl Violet Acetate) 0.5 g Sodium acetate (CH3COONa?3H2O) 0.18 g Distilled water 500.0 mL Acetic acid, concentrated (CH3COOH) 1.5 mL (up to) Dissolve cresyl echt violet and sodium acetate in distilled water. Slowly add acetic acid, drop by drop, to solution. Should have a pH of 3.5. If solution pH is below 3.5, add more sodium acetate. If solution pH is above 3.5, add more acetic acid. Filter. Let stand overnight before using. Store at room temperature. Stable for months. May be reused until weak. Stain for 1-2 hours. Differentiate in 2 changes 95% alcohol, until background cytoplasm is clear (check with microscope). Run up through 2 changes 100% reagent alcohol, 2 changes xylene or substitute, coverslip. Nissl, RNA and DNA (nuclei) will be purple. To speed up differentiation, and to pull more out of the DNA/nuclei, add 2 drops of acetic acid conc. to the first 95% alcohol. It might be CI # 51180, but I don't have that info at home. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital 3601 W. 13 Mile Rd. Royal Oak, MI 48073 Opinions expressed by me to not represent my place of employment. -----Original Message----- From: Yaskovich, Ruth A (NIH/NIDCR) [E] Sent: Thursday, May 02, 2013 3:52 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cresyl echt Violett for Nissl substance What is everyone using for this stain? Ruth Yaskovich N.I.H. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ASelf <@t> georgetownhospitalsystem.org Wed May 8 07:35:17 2013 From: ASelf <@t> georgetownhospitalsystem.org (Amy Self) Date: Wed May 8 07:35:31 2013 Subject: [Histonet] Scabies Procedure Message-ID: Good Morning All, Does anyone have a scabies procedure that they could send to me. This is not a procedure that we routinely perform but we have a child in the OR that the surgeon is requesting scabies testing on. Thanks in advance for nay information. Amy Self GMH Hospital NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. From rheyna <@t> lumc.edu Wed May 8 10:40:38 2013 From: rheyna <@t> lumc.edu (Roger Heyna) Date: Wed May 8 10:40:45 2013 Subject: [Histonet] H pylori and Ventana instruments In-Reply-To: <20130507135608.jn2udaa9ulssokgk@webmail2.centurytel.net> References: <20130507135608.jn2udaa9ulssokgk@webmail2.centurytel.net> Message-ID: <518A2BA6020000230004E88E@gwgwia1.luhs.org> We use Ventana's HP antibody, and we do see some of the small, non-specific globs of stain, but the pathologists are comfortable differentiating between the true and false staining since they are familiar with this artefact. Roger Heyna >>> "Rae Staskiewicz" 5/7/2013 12:56 PM >>> Hi all, Those of you using Ventana instrumentation, could you tell me what H pylori antibody you are using, and whether or not you are experiencing non-specific staining, or staining that looks specific but with a stippled effect. Thanks Rae Staskiewicz UnityPoint Health-Methodist _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ttruscot <@t> vetmed.wsu.edu Wed May 8 10:57:54 2013 From: ttruscot <@t> vetmed.wsu.edu (Truscott, Tom) Date: Wed May 8 10:58:00 2013 Subject: [Histonet] H pylori and Ventana instruments In-Reply-To: <518A2BA6020000230004E88E@gwgwia1.luhs.org> References: <20130507135608.jn2udaa9ulssokgk@webmail2.centurytel.net> <518A2BA6020000230004E88E@gwgwia1.luhs.org> Message-ID: <9EF5279EBDFE6E4FB6605E8F183A00274B635A69@CVM76.vetmed.wsu.edu> I haven't used HP, but have noticed this stippled effect especially when using 40X on the microscope and looking at slides labeled with an Ventana alk-phos kit. I seem to have solved the problem by making sure no trace of H2O is left on the slides. It helps after washing slides to run them thru a few changes of alcohol then one acetone and then a couple xylene before coverslipping. Ventana suggested the acetone step. Tom T -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Roger Heyna Sent: Wednesday, May 08, 2013 8:41 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] H pylori and Ventana instruments We use Ventana's HP antibody, and we do see some of the small, non-specific globs of stain, but the pathologists are comfortable differentiating between the true and false staining since they are familiar with this artefact. Roger Heyna >>> "Rae Staskiewicz" 5/7/2013 12:56 PM >>> Hi all, Those of you using Ventana instrumentation, could you tell me what H pylori antibody you are using, and whether or not you are experiencing non-specific staining, or staining that looks specific but with a stippled effect. Thanks Rae Staskiewicz UnityPoint Health-Methodist _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From one_angel_secret <@t> yahoo.com Wed May 8 11:16:05 2013 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Wed May 8 11:16:10 2013 Subject: [Histonet] IHC/Grossing Tech wanted in SW Florida Message-ID: <1368029765.70229.YahooMailNeo@web161602.mail.bf1.yahoo.com> Hi Histonetters, ??????????????????????????? We are looking for a part time evening Histologist to do IHC and light grossing in the Fort Myers Florida area.?Riverchase Dermatology?is the largest most comprehensive?skin care center?in the area with over 6 offices and growing. Here is a link to view our website. http://www.riverchasedermatology.com/ ? If you are interested in joining our fun/energetic/awesome team and meet CLIA regulations for high complexity testing, please email me with your resume. ? Sincerely, ? Kim Donadio HTL Lab/Histology Supervisor Riverchase Dermatology email: kdonadio@cfdcs.com From wbenton <@t> cua.md Wed May 8 12:39:01 2013 From: wbenton <@t> cua.md (Walter Benton) Date: Wed May 8 12:39:07 2013 Subject: [Histonet] FYI Regarding HT and HTL Message-ID: <0B8979A204680A42B93A52B486088CD93698C8BFBE@CUAEXH1.GCU-MD.local> http://naaclsnews.wordpress.com/2013/05/03/proposed-change-to-htl-standards/ Proposed Change to 2012 HTL Standards: Request for Public Comment In September 2012, the NAACLS Board of Directors approved new Standards for Accredited and Approved Programs. It is projected that programs will begin to be reviewed under these Standards starting with those programs that will be submitting their self-studies on or after September 1st, 2014. The 2012 Standards for Accredited Histotechnologist (HTL) programs necessitate that the program director (or education coordinator, when required) hold ?ASCP-BOC certification as a Histotechnologist?. This is a change from the previous HTL Standard for program directors (approved in 2004), which stated that the program director (or education coordinator, when required) must be ?certified in Histotechnology?. NAACLS? interpretation and practice of the old standard allowed for those with HTL(ASCP) or HT(ASCP) certification to be the program director or education coordinator of an HTL program. The NAACLS Board of Directors is requesting public comment for a proposed 2012 Standards change to 2012 HTL Standard 7A1B and 2012 HTL Standard 7B1B . This change will allow both HTL(ASCP) and HT(ASCP) certifications to be acceptable for program directors and education coordinators of NAACLS Accredited HTL programs, as recommended by the Review Committee for Accredited Programs. This period of public comment has been made available for thirty (30) days. The current 2012 HTL Standard 7A1B reads as follows: ??holds ASCP-BOC U.S. certification as a Histotechnologist. Program Directors who have been approved as a program director of a NAACLS accredited HTL program prior to October 1, 2013 remain eligible as a program director. If the program director does not hold ASCP-BOC U.S. certification as a Histotechnologist, a qualified professional who does hold ASCP-BOC U.S. certification as a Histotechnologist must hold appointment as education coordinator.? The proposed change to 2012 HTL Standard 7A1B reads as follows: ??holds ASCP-BOC U.S. certification as a Histotechnologist or Histotechnician. Program Directors who have been approved as a program director of a NAACLS accredited HTL program prior to October 1, 2013 remain eligible as a program director. If the program director does not hold ASCP-BOC U.S. certification as a Histotechnologist or Histotechnician, a qualified professional who does hold ASCP-BOC U.S. certification as a Histotechnologist or Histotechnician must hold appointment as education coordinator.? The current 2012 HTL Standard 7B1B reads as follows: ??holds ASCP-BOC U.S. certification as a Histotechnologist.? The proposed change to 2012 HTL Standard 7B1B reads as follows: ??holds ASCP-BOC U.S. certification as a Histotechnologist or Histotechnician.? This request for public comment has been placed on the NAACLS website, and comments may be sent via letter to the NAACLS office, or by email to erotchford@naacls.org. Due to the informal nature of LISTSERV communications, comments or discussion posted on the CLS Educators LISTSERV will not be presented to or considered by the NAACLS Board of Directors. All comments must be received by June 3, 2013. The comments will then be reviewed and brought before the Board of Directors at the September 2013 Board Meeting. Edward Rotchford, Accreditation Specialist Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) wbenton@cua.md ________________________________ CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From joelleweaver <@t> hotmail.com Wed May 8 13:11:15 2013 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Wed May 8 13:11:20 2013 Subject: [Histonet] FYI Regarding HT and HTL In-Reply-To: <0B8979A204680A42B93A52B486088CD93698C8BFBE@CUAEXH1.GCU-MD.local> References: <0B8979A204680A42B93A52B486088CD93698C8BFBE@CUAEXH1.GCU-MD.local> Message-ID: Thanks for sharing with everyone who may be impacted so they can comment. I have provided my opinion already.... Joelle Weaver MAOM, HTL (ASCP) QIHC > From: wbenton@cua.md > To: histonet@lists.utsouthwestern.edu > Date: Wed, 8 May 2013 13:39:01 -0400 > Subject: [Histonet] FYI Regarding HT and HTL > > http://naaclsnews.wordpress.com/2013/05/03/proposed-change-to-htl-standards/ > > Proposed Change to 2012 HTL Standards: Request for Public Comment > In September 2012, the NAACLS Board of Directors approved new Standards for Accredited and Approved Programs. It is projected that programs will begin to be reviewed under these Standards starting with those programs that will be submitting their self-studies on or after September 1st, 2014. > The 2012 Standards for Accredited Histotechnologist (HTL) programs necessitate that the program director (or education coordinator, when required) hold ?ASCP-BOC certification as a Histotechnologist?. This is a change from the previous HTL Standard for program directors (approved in 2004), which stated that the program director (or education coordinator, when required) must be ?certified in Histotechnology?. NAACLS? interpretation and practice of the old standard allowed for those with HTL(ASCP) or HT(ASCP) certification to be the program director or education coordinator of an HTL program. > The NAACLS Board of Directors is requesting public comment for a proposed 2012 Standards change to 2012 HTL Standard 7A1B and 2012 HTL Standard 7B1B . This change will allow both HTL(ASCP) and HT(ASCP) certifications to be acceptable for program directors and education coordinators of NAACLS Accredited HTL programs, as recommended by the Review Committee for Accredited Programs. This period of public comment has been made available for thirty (30) days. > > The current 2012 HTL Standard 7A1B reads as follows: > ??holds ASCP-BOC U.S. certification as a Histotechnologist. Program Directors who have been approved as a program director of a NAACLS accredited HTL program prior to October 1, 2013 remain eligible as a program director. If the program director does not hold ASCP-BOC U.S. certification as a Histotechnologist, a qualified professional who does hold ASCP-BOC U.S. certification as a Histotechnologist must hold appointment as education coordinator.? > The proposed change to 2012 HTL Standard 7A1B reads as follows: > ??holds ASCP-BOC U.S. certification as a Histotechnologist or Histotechnician. Program Directors who have been approved as a program director of a NAACLS accredited HTL program prior to October 1, 2013 remain eligible as a program director. If the program director does not hold ASCP-BOC U.S. certification as a Histotechnologist or Histotechnician, a qualified professional who does hold ASCP-BOC U.S. certification as a Histotechnologist or Histotechnician must hold appointment as education coordinator.? > The current 2012 HTL Standard 7B1B reads as follows: > ??holds ASCP-BOC U.S. certification as a Histotechnologist.? > The proposed change to 2012 HTL Standard 7B1B reads as follows: > ??holds ASCP-BOC U.S. certification as a Histotechnologist or Histotechnician.? > > This request for public comment has been placed on the NAACLS website, and comments may be sent via letter to the NAACLS office, or by email to erotchford@naacls.org. Due to the informal nature of LISTSERV communications, comments or discussion posted on the CLS Educators LISTSERV will not be presented to or considered by the NAACLS Board of Directors. All comments must be received by June 3, 2013. The comments will then be reviewed and brought before the Board of Directors at the September 2013 Board Meeting. > Edward Rotchford, Accreditation Specialist > Walter Benton HT(ASCP)QIHC > Histology Supervisor > Chesapeake Urology Associates > 806 Landmark Drive, Suite 127 > Glen Burnie, MD 21061 > 443-471-5850 (Direct) > 410-768-5961 (Lab) > 410-768-5965 (Fax) > wbenton@cua.md > > > ________________________________ > CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Margaret.Perry <@t> sdstate.edu Wed May 8 14:25:21 2013 From: Margaret.Perry <@t> sdstate.edu (Perry, Margaret) Date: Wed May 8 14:25:29 2013 Subject: [Histonet] S100 Message-ID: <25F4FBA34BE9D142964ECC4525B82AEE956E10B0@SDSU-EX03.jacks.local> Our S100 is not working very well. I think it's too old. Can someone recommend an S100 antibody that works well in dog? It would be really nice to find a good monoclonal instead of the polyclonals. We are looking for schwannomas, astrogliomas, malignant melanomas and their metastases. Margaret Perry HT(ASCP) Veterinary & Biomedical Sciences Department North Campus Drive Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 From akemiat3377 <@t> yahoo.com Wed May 8 15:10:37 2013 From: akemiat3377 <@t> yahoo.com (Akemi Allison) Date: Wed May 8 15:10:42 2013 Subject: [Histonet] Giardia controls in exchange for H-Pylori controls Message-ID: <1368043837.77443.YahooMailNeo@web140604.mail.bf1.yahoo.com> Hello Everyone in Histoland! ? I would like to reach out to you to see if anyone out there would be willing to exchange some Giardia Controls for H-Pylori contols. Please let me know as soon as possible.? My pathology director has requested me to start doing research on duodenum polyps to check for Giardia using a Giemsa method.? ? Thank you in advance, Akemi ? Akemi Allison B.S., HT/HTL (ASCP) Pathology Manager Monterey Bay GI Consultants Medical Group 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 (8310 375-3577 x 117 Email: aallison@montereygi.com From akemiat3377 <@t> yahoo.com Wed May 8 15:35:11 2013 From: akemiat3377 <@t> yahoo.com (Akemi Allison) Date: Wed May 8 15:35:16 2013 Subject: [Histonet] Attention GI facilities-(Giardia) Statistical Information on Duodenum polyps stained Message-ID: <1368045311.89746.YahooMailNeo@web140604.mail.bf1.yahoo.com> Hello again everyone: ? The medical director of pathology at my facility asked to make an inquiry on Histonet regarding any statistical information?of?special staining on duodenum polyps for Giardia routinely. Do you have any statistics, and if so, do you have the percentages too?? Any information would be greatly appreciated.? Also, do you do these special stains on everyone of your duodenum polyps?We?do not want to do IHC, but prefer special stains because of the cost factor. ? ? ? ? I used to do a Giemsa modification years ago, but he would like to know if there are any of you doing other special stains for Giardia.? We are trying to avoid the more complicated silver stains such as Steiner or Warthin Starry. ? ? Thank you ? Akemi ? ? ? ? ? ? ? Akemi Allison B.S., HT/HTL (ASCP) Pathology Manager Monterey Bay GI Consultants Medical Group 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 (8310 375-3577 x 117 Email: aallison@montereygi.com From akemiat3377 <@t> yahoo.com Wed May 8 16:41:38 2013 From: akemiat3377 <@t> yahoo.com (Akemi Allison) Date: Wed May 8 16:41:45 2013 Subject: [Histonet] Fw: Minor clarification Attention GI facilities-(Giardia) Statistical Information on Duodenum polyps stained In-Reply-To: <1368045311.89746.YahooMailNeo@web140604.mail.bf1.yahoo.com> References: <1368045311.89746.YahooMailNeo@web140604.mail.bf1.yahoo.com> Message-ID: <1368049298.60868.YahooMailNeo@web140604.mail.bf1.yahoo.com> ?? Sorry,?Just to make a minor clarification.? Duodenal biopsies, not polypectomies.? ? Thanks for all of your efforts! Akemi Allison B.S., HT/HTL (ASCP) Pathology Manager Monterey Bay GI Consultants Medical Group 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 (8310 375-3577 x 117 Email: aallison@montereygi.com ? Original Below The medical director of pathology at my facility asked to make an inquiry on Histonet regarding any statistical information?of?special staining on duodenum polyps for Giardia routinely. Do you have any statistics, and if so, do you have the percentages too?? Any information would be greatly appreciated.? Also, do you do these special stains on everyone of your duodenum polyps?We?do not want to do IHC, but prefer special stains because of the cost factor. ? ? ? ? I used to do a Giemsa modification years ago, but he would like to know if there are any of you doing other special stains for Giardia.? We are trying to avoid the more complicated silver stains such as Steiner or Warthin Starry. ? ? Thank you ? Akemi ? ? ? ? ? ? ? Akemi Allison B.S., HT/HTL (ASCP) Pathology Manager Monterey Bay GI Consultants Medical Group 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 (8310 375-3577 x 117 Email: aallison@montereygi.com From shive003 <@t> umn.edu Wed May 8 20:23:44 2013 From: shive003 <@t> umn.edu (Jan Shivers) Date: Wed May 8 20:23:52 2013 Subject: [Histonet] S100 In-Reply-To: <25F4FBA34BE9D142964ECC4525B82AEE956E10B0@SDSU-EX03.jacks.local> References: <25F4FBA34BE9D142964ECC4525B82AEE956E10B0@SDSU-EX03.jacks.local> Message-ID: Margaret, What S100 are you using? We use Dako's polyclonal, no antigen retrieval needed, with no problems. Jan Shivers UMN VDL On Wed, May 8, 2013 at 2:25 PM, Perry, Margaret wrote: > Our S100 is not working very well. I think it's too old. Can someone > recommend an S100 antibody that works well in dog? It would be really nice > to find a good monoclonal instead of the polyclonals. > We are looking for schwannomas, astrogliomas, malignant melanomas and > their metastases. > > Margaret Perry HT(ASCP) > Veterinary & Biomedical Sciences Department > North Campus Drive Box 2175 > South Dakota State University > Brookings SD 57007 > 605-688-5638 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From delsuec <@t> gmail.com Thu May 9 09:45:18 2013 From: delsuec <@t> gmail.com (Deloris Carter) Date: Thu May 9 09:45:27 2013 Subject: [Histonet] Mopec Medite Embedding Center Parts Message-ID: Hi, I'm wondering if anyone out there in Histoland has a source for parts for the Mopec Medite Embedding Center. The paraffin dispensing switch is going out of ours, and our BioMed department has had no luck finding a replacement part. I appreciate any help. Deloris Carter delsuec@gmail.com From ranostaj <@t> medite-group.com Thu May 9 10:55:30 2013 From: ranostaj <@t> medite-group.com (Drew Ranostaj) Date: Thu May 9 10:55:37 2013 Subject: [Histonet] Mopec Medite Embedding Center Parts In-Reply-To: References: Message-ID: Hi Deloris, Please contact our Service Department at 407-996-9630 and they will be able to help you directly. If you need anything else, please let me know! Best Regards, Drew Ranostaj National Sales Manager Medite Inc. 1226 Winter Garden Vineland Rd, Suite 104 Winter Garden, Florida 34787, U.S.A. Phone: +1 (407) 996 9630 Fax: +1 (407) 996 9631 Toll Free: 888 225 2950 E-mail: ranostaj@medite-group.com http://www.medite-group.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Deloris Carter Sent: Thursday, May 09, 2013 10:45 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Mopec Medite Embedding Center Parts Hi, I'm wondering if anyone out there in Histoland has a source for parts for the Mopec Medite Embedding Center. The paraffin dispensing switch is going out of ours, and our BioMed department has had no luck finding a replacement part. I appreciate any help. Deloris Carter delsuec@gmail.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From KarynMyers <@t> texashealth.org Thu May 9 12:26:13 2013 From: KarynMyers <@t> texashealth.org (Myers, Karyn) Date: Thu May 9 12:26:19 2013 Subject: [Histonet] Re-use of plastic embedding molds Message-ID: Just wondering....... Does anyone re-use their plastic embedding base molds? Thanks for your input! The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is PRIVILEGED, CONFIDENTIAL, and exempt from disclosure under applicable law. If you are not the intended recipient, you are prohibited from copying, distributing, or using the information. Please contact the sender immediately by return e-mail and delete the original message from your system. From wdesalvo.cac <@t> outlook.com Thu May 9 13:03:52 2013 From: wdesalvo.cac <@t> outlook.com (WILLIAM DESALVO) Date: Thu May 9 13:03:57 2013 Subject: [Histonet] Re-use of plastic embedding molds In-Reply-To: References: Message-ID: We tried using the disposable molds and they work for reusing 2-3 times( cracks started after just a few uses), but we found the cost much higher that using the metal molds and cleaning. William DeSalvo, BS HTL(ASCP) Production Manager-Anatomic Pathology Chair, NSH Quality Management Committee Owner/Consultant, Collaborative Advantage Consulting > From: KarynMyers@texashealth.org > To: histonet@lists.utsouthwestern.edu > Date: Thu, 9 May 2013 17:26:13 +0000 > Subject: [Histonet] Re-use of plastic embedding molds > > Just wondering....... Does anyone re-use their plastic embedding base molds? > > Thanks for your input! > > > > The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is PRIVILEGED, CONFIDENTIAL, and exempt from disclosure under applicable law. If you are not the intended recipient, you are prohibited from copying, distributing, or using the information. Please contact the sender immediately by return e-mail and delete the original message from your system. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpwenk <@t> sbcglobal.net Thu May 9 18:45:21 2013 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Thu May 9 18:45:22 2013 Subject: [Histonet] Holzer method for glial fibers In-Reply-To: <1367796315.78613.YahooMailNeo@web120406.mail.ne1.yahoo.com> References: <1367796315.78613.YahooMailNeo@web120406.mail.ne1.yahoo.com> Message-ID: Some time ago, someone asked what all the reagents in the Holzer stain were for, and John Kiernan made some guess (see in quotes below for his response to the PMA). If John KIernan is having to make educated guesses, the rest of us probably have NO IDEA. Added to that, I don't know anyone who does this stain anymore. If we need to demonstrate glial cells, everyone is doing IHC such as GFAP. John's response: "I don't think there are answers, other than wild speculation, to your questions! A few statements can be made about the reagents, but they don't necessarily relate to Holzer's method. When a PMA solution is applied to a section its large anions stick to regions of protein that are permeable and rich in basic amino acids. (This happens in collagen, in trichrome methods.) PMA also makes insoluble pigment precipitates when mixed with cationic triphenylmethane dyes such as crystal violet. Non-aqueous PMA may have tissue affinity different from that of an aqueous solution. With the chemically related phosphotungstic acid, the solvent and other factors influence the tissue components that take up the metal when PTA is used as a contrast stain for EM (see Hayat 1993 Stains and Cytochemical Methods, various places in book)." Back to my opinion: I'm really worried about some of the other reagents, and anyone who has to use them: - Chloroform - EXTREMELY flammable - Aniline oil - EXTREMELY carcinogenic (bladder cancer), and causes liver and hematopoetic damage Is there any way you can convince your instructor to let you do a stain that is still in use, clinically relevant, and SAFER!?!?!?!?! Peggy A. Wenk, HTL(ASCP)SLS Program Director Schools of Histotechnology Beaumont Health System 3601 W. 13 Mile Road Royal Oak, MI 48073 Opinions expressed are my own, and do not reflect my place of employment. -----Original Message----- From: jesse andrade Sent: Sunday, May 05, 2013 7:25 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Holzer method for glial fibers I have a question about this method and the purpose of the phosphomolybdic acid-alcohol solution in this stain? I am astudent and need this for my staining procedure assignment. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Susan.Walzer <@t> HCAHealthcare.com Fri May 10 01:44:56 2013 From: Susan.Walzer <@t> HCAHealthcare.com (Susan.Walzer@HCAHealthcare.com) Date: Fri May 10 01:45:05 2013 Subject: [Histonet] Re-use of plastic embedding molds In-Reply-To: References: Message-ID: <4BF03F5404EBDE409AF9232DA74B9DED2F9D360426@FWDCWPMSGCMS09.hca.corpad.net> We tried them but they have a tendency to warp and you will not get a flat surface for cutting. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO Sent: Thursday, May 09, 2013 2:04 PM To: Myers, Karyn; histonet Subject: RE: [Histonet] Re-use of plastic embedding molds We tried using the disposable molds and they work for reusing 2-3 times( cracks started after just a few uses), but we found the cost much higher that using the metal molds and cleaning. William DeSalvo, BS HTL(ASCP) Production Manager-Anatomic Pathology Chair, NSH Quality Management Committee Owner/Consultant, Collaborative Advantage Consulting > From: KarynMyers@texashealth.org > To: histonet@lists.utsouthwestern.edu > Date: Thu, 9 May 2013 17:26:13 +0000 > Subject: [Histonet] Re-use of plastic embedding molds > > Just wondering....... Does anyone re-use their plastic embedding base molds? > > Thanks for your input! > > > > The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is PRIVILEGED, CONFIDENTIAL, and exempt from disclosure under applicable law. If you are not the intended recipient, you are prohibited from copying, distributing, or using the information. Please contact the sender immediately by return e-mail and delete the original message from your system. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tgenade <@t> gmail.com Fri May 10 01:56:43 2013 From: tgenade <@t> gmail.com (Tyrone Genade) Date: Fri May 10 01:56:49 2013 Subject: [Histonet] Re: Histonet Digest, Vol 114, Issue 8 In-Reply-To: <518bd634.a7243c0a.5741.ffffb02aSMTPIN_ADDED_MISSING@mx.google.com> References: <518bd634.a7243c0a.5741.ffffb02aSMTPIN_ADDED_MISSING@mx.google.com> Message-ID: On Wed, 8 May 2013 19:25:21, "Perry, Margaret" wrote: > Our S100 is not working very well. I think it's too old. Can someone recommend an S100 > antibody that works well in dog? It would be really nice to find a good monoclonal instead > of the polyclonals. > We are looking for schwannomas, astrogliomas, malignant melanomas and their > metastases. How are you processing your tissue? S100 is very soluble and can wash out of unfixed frozen sections. I only achieve stating, using the DAKO anti-S100 polyclonal, if I thoroughly fix my brains first in PFA. The DAKO anti-S100 is a diagnostic antibody so it should give no problems unless, as you suspect, it is very old. But then it has to be VERY old as I am using antibody aliquots which have been stored at -20 oC that are now 6 years old and regularly retrieve antibodies from -80 that have been there for more than 20 years. If the antibody has been stored at 4 oC then it could be past its usefulness... But even then, many are very stable and can last years. Kind regards -- Tyrone Genade Ph.D. Department of Human Biology University of Cape Town http://tgenade.freeshell.org email: tgenade@gmail.com tel: +27-84-632-1925 (c) ******************************************************************************** Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord. To find out how to receive this FREE gift visit http://www.alpha.org. From LTurner1 <@t> seton.org Fri May 10 07:05:34 2013 From: LTurner1 <@t> seton.org (Turner, Leandra) Date: Fri May 10 07:05:40 2013 Subject: [Histonet] ANP.21382-Evaluating reagents lacking exp. date Message-ID: Hi Everyone, This CAP question has probably been posted before, so I apologize in advance. But I was wondering if anyone would be willing to share some ideas about the best way to handle this question. Thank You, Leandra Turner, HT (ASCP)cm Dell Children's Medical Center CONFIDENTIALITY NOTICE: This email message and any accompanying data or files is confidential and may contain privileged information intended only for the named recipient(s). If you are not the intended recipient(s), you are hereby notified that the dissemination, distribution, and or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at the email address above, delete this email from your computer, and destroy any copies in any form immediately. Receipt by anyone other than the named recipient(s) is not a waiver of any attorney-client, work product, or other applicable privilege. From Timothy.Morken <@t> ucsfmedctr.org Fri May 10 10:04:12 2013 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Fri May 10 10:04:26 2013 Subject: [Histonet] RE: ANP.21382-Evaluating reagents lacking exp. date In-Reply-To: References: Message-ID: <761E2B5697F795489C8710BCC72141FF07E0D4@ex07.net.ucsf.edu> We label liquids with a 3-year expiration and solids with a 5 year expiration. I try to order amounts that we will use it up within that time period (tho usually a shorter time period for most things, like a year or less). If not, we run a test on the reagent and relabel it for another 3 or 5 years. I also asked that question to Fisher Scientific tech support and they sent me a letter stating that reagents without expiration dates were expected to last indefinitely under the stated storage conditions, but generally 3 to 5 years was a reasonable assumption for shelf life. One thing you can do to ensure shelf life of powders is order hydrated versions. Anhydrous versions will absorb water from the air over time and so have less shelf life. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Turner, Leandra Sent: Friday, May 10, 2013 5:06 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ANP.21382-Evaluating reagents lacking exp. date Hi Everyone, This CAP question has probably been posted before, so I apologize in advance. But I was wondering if anyone would be willing to share some ideas about the best way to handle this question. Thank You, Leandra Turner, HT (ASCP)cm Dell Children's Medical Center CONFIDENTIALITY NOTICE: This email message and any accompanying data or files is confidential and may contain privileged information intended only for the named recipient(s). If you are not the intended recipient(s), you are hereby notified that the dissemination, distribution, and or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at the email address above, delete this email from your computer, and destroy any copies in any form immediately. Receipt by anyone other than the named recipient(s) is not a waiver of any attorney-client, work product, or other applicable privilege._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMaslanka <@t> stpetes.org Fri May 10 10:35:12 2013 From: JMaslanka <@t> stpetes.org (JMaslanka@stpetes.org) Date: Fri May 10 10:36:32 2013 Subject: [Histonet] Vantage and Prisma Message-ID: Happy Friday Anyone out there using Ventana's Vantage with Sakura's Tissue-Tek Prisma stainer? Joe Maslanka BS, CT,HT (ASCP) Anatomical Pathology Technical Supervisor St Peter's Hospital,MT 59601 (P)(406) 447-2406 (F)(406)444-2126 Give thanks for ALL things..... "Kindness is the language the blind can see & the deaf can hear- Mark Twain This electronic mail message contains information which is confidential. If you are not the intended recipient, please be aware that any disclosure, photocopying, distribution or use of the contents of the received information is prohibited. If you have received this e-mail in error, please reply to the sender immediately and permanently delete this message and all copies of it. Thank you. From Timothy.Morken <@t> ucsfmedctr.org Fri May 10 10:46:23 2013 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Fri May 10 10:46:32 2013 Subject: [Histonet] Vantage and Prisma In-Reply-To: References: Message-ID: <761E2B5697F795489C8710BCC72141FF07E10A@ex07.net.ucsf.edu> Joe, since the Prisma can't read bar codes you will need to label the slides before staining (usually at the microtome, which also acts as a checkpoint between cutting and staining) and then hand-scan them after they come out of the stainer. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of JMaslanka@stpetes.org Sent: Friday, May 10, 2013 8:35 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Vantage and Prisma Happy Friday Anyone out there using Ventana's Vantage with Sakura's Tissue-Tek Prisma stainer? Joe Maslanka BS, CT,HT (ASCP) Anatomical Pathology Technical Supervisor St Peter's Hospital,MT 59601 (P)(406) 447-2406 (F)(406)444-2126 Give thanks for ALL things..... "Kindness is the language the blind can see & the deaf can hear- Mark Twain This electronic mail message contains information which is confidential. If you are not the intended recipient, please be aware that any disclosure, photocopying, distribution or use of the contents of the received information is prohibited. If you have received this e-mail in error, please reply to the sender immediately and permanently delete this message and all copies of it. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jo-ann.bader <@t> mcgill.ca Fri May 10 11:17:33 2013 From: jo-ann.bader <@t> mcgill.ca (Jo-Ann Bader, Ms.) Date: Fri May 10 11:17:37 2013 Subject: [Histonet] Fibrin staining Message-ID: <8C36045F0065CE48906E684F15FD4CB6320BDB32@EXMBX2010-6.campus.MCGILL.CA> We have a client who is interested in studying both fibrin loose in the mouse tissue as well as intravascular (arterial or venous). It has been suggested I do either a Picro-Mallory or a Martius, Scarlet & Blue (MSB) stain. Is there any other stains I could do, which is the most popular? Where could I get either control blocks or slides. I have been looking but have not come across any yet. Thanks Jo-Ann Bader Histology Co-Ordinator Goodman Cancer Centre McGill University 1160 Pine Ave. W - Rm 312 Montreal, QC, Canada H3G 1Y6 Tel: 514-398-8270 From lcolbert <@t> pathmdlabs.com Fri May 10 12:10:59 2013 From: lcolbert <@t> pathmdlabs.com (Laurie Colbert) Date: Fri May 10 12:12:08 2013 Subject: [Histonet] Full Time Openings for Histotech and Grossing Tech Message-ID: <12ECD7346266D74691EC2BFC75285E452F2DDD4B@BFL323E10.pathmdlabs.local> PATH MD has two full time openings for a histotech and a grossing tech. Both positions require at least two years of experience and the ability to troubleshoot a variety of issues. The histotechnician shift is from 11:00pm to 7:30 am and entails embedding, cutting, and manual special stains. The grossing tech shift is 10:30 am to 7:00 pm, Monday-Friday. PATH MD is a new pathology reference lab located in West Hollywood, CA. We are rapidly expanding and are looking to add motivated and experienced personnel to our team. Salary is competitive and full medical, dental, and vision benefits are offered. If interested, please forward your resume to: lcolbert@pathmdlabs.com Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab From wdesalvo.cac <@t> outlook.com Fri May 10 12:18:50 2013 From: wdesalvo.cac <@t> outlook.com (WILLIAM DESALVO) Date: Fri May 10 12:18:54 2013 Subject: [Histonet] Vantage and Prisma In-Reply-To: References: Message-ID: I use Vantage and Sakura Prisma. The Sakura Prisma is not interfaced into Vantage. You place a bar-coded label on your H&E slides at microtomy. We scan the slides after staining, case assembly. William DeSalvo, BS HTL(ASCP) Production Manager-Anatomic Pathology Chair, NSH Quality Management Committee Owner/Consultant, Collaborative Advantage Consulting > To: histonet@lists.utsouthwestern.edu > From: JMaslanka@stpetes.org > Date: Fri, 10 May 2013 09:35:12 -0600 > Subject: [Histonet] Vantage and Prisma > > Happy Friday > Anyone out there using Ventana's Vantage with Sakura's Tissue-Tek Prisma > stainer? > > > Joe Maslanka BS, CT,HT (ASCP) > Anatomical Pathology Technical Supervisor > St Peter's Hospital,MT 59601 > (P)(406) 447-2406 > (F)(406)444-2126 > > Give thanks for ALL things..... > "Kindness is the language the blind can see & the deaf can hear- Mark > Twain > > > > This electronic mail message contains information which is confidential. > If you are not the intended recipient, please be aware that any > disclosure, photocopying, distribution or use of the contents of the > received information is prohibited. If you have received this e-mail in > error, please reply to the sender immediately and permanently delete this > message and all copies of it. Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jtaylor <@t> meriter.com Fri May 10 12:53:45 2013 From: jtaylor <@t> meriter.com (Taylor, Jean) Date: Fri May 10 12:53:54 2013 Subject: [Histonet] p40 Message-ID: Hi everyone, I'm looking into ordering the p40 antibody for one of our pathologists. He wants to use it to help distinguish between adenocarcinoma and squamous cell ca in lung. I'm wondering what labs are using, the monoclonal or polyclonal antibody, and where you purchase it from. I haven't found that a lot of companies that carry it. Any info would be helpful. Thanks, Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI From AWalton <@t> oima.org Fri May 10 13:02:30 2013 From: AWalton <@t> oima.org (Annie T. Walton) Date: Fri May 10 13:02:41 2013 Subject: [Histonet] GI biopsy H&E staining Message-ID: Hello all, Our Medical Director would like the goblet cells on our GI biopsies to stain a darker blue. I hand stain using the following protocol: * Xylene, 3 changes 3 minutes each * 100% Alcohol, 2 changes 1 minute each * 95% Alcohol 1 minute * Tap water rinse until water runs off evenly * Harris Hematoxylin 5 minutes * Water wash rinse until water runs clear * Clarifier 2 5 seconds * Running water 30 seconds * Bluing Solution 1 minute * Tap water rinse well * 95% Alcohol 30 seconds * Eosin Y 1 minute * 100% Alcohol, 2 changes 30 seconds * 100% Alcohol, 2 changes 1 minute each * Xylene, 3 changes 1 minute each I have recently increased the hematoxylin time from 4 to 5 minutes and decreased the time in clarifier from 25 to 5 seconds so the hematoxylin doesn't get washed out as much, but it is not good enough. I also switched from 70% alcohol to 95% before eosin and increased the time in eosin from 45 seconds to 1 minute to provide a greater contrast in hopes that that would work, but the goblet cells are still not staining dark enough. Any suggestions would greatly be appreciated! Thanks! Annie Walton, HTL (ASCP) Histology Technician Overlake Internal Medicine Associates Histology Lab 425-974-9643 awalton@oima.org ________________________________ DISCLAIMER: This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution or copying of this information is strictly prohibited. If you received this message in error, please notify the sender then delete this message. From JMacDonald <@t> mtsac.edu Fri May 10 13:33:19 2013 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Fri May 10 13:33:28 2013 Subject: [Histonet] GI biopsy H&E staining In-Reply-To: Message-ID: Use a Gill hematoxylin. Sent from my iPad On May 10, 2013, at 11:05 AM, "Annie T. Walton" wrote: > Hello all, > > Our Medical Director would like the goblet cells on our GI biopsies to stain a darker blue. I hand stain using the following protocol: > > > * Xylene, 3 changes 3 minutes each > * 100% Alcohol, 2 changes 1 minute each > * 95% Alcohol 1 minute > * Tap water rinse until water runs off evenly > * Harris Hematoxylin 5 minutes > * Water wash rinse until water runs clear > * Clarifier 2 5 seconds > * Running water 30 seconds > * Bluing Solution 1 minute > * Tap water rinse well > * 95% Alcohol 30 seconds > * Eosin Y 1 minute > * 100% Alcohol, 2 changes 30 seconds > * 100% Alcohol, 2 changes 1 minute each > * Xylene, 3 changes 1 minute each > > I have recently increased the hematoxylin time from 4 to 5 minutes and decreased the time in clarifier from 25 to 5 seconds so the hematoxylin doesn't get washed out as much, but it is not good enough. I also switched from 70% alcohol to 95% before eosin and increased the time in eosin from 45 seconds to 1 minute to provide a greater contrast in hopes that that would work, but the goblet cells are still not staining dark enough. Any suggestions would greatly be appreciated! > > Thanks! > > Annie Walton, HTL (ASCP) > Histology Technician > Overlake Internal Medicine Associates Histology Lab > 425-974-9643 > awalton@oima.org > > > ________________________________ > DISCLAIMER: This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution or copying of this information is strictly prohibited. If you received this message in error, please notify the sender then delete this message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From KSimeone <@t> leavittmgt.com Fri May 10 13:46:36 2013 From: KSimeone <@t> leavittmgt.com (Delray Beach Pathology Kari Simeone) Date: Fri May 10 13:46:49 2013 Subject: [Histonet] Extra Ventana Reagent Message-ID: Hi everyone. I use the Ventana NexES and when opening a new kit for PAS, I end up tossing the extra (2) Schiff's 2 (22ml each) bottles. Is there anyone out there that can use this stuff? I'd love to help someone instead of just wasting it. I inquired with Ventana and they basically told me that's just how they sell it. Yuck, wasteful. It's already registered so of no use on any other instrument but who knows?? Kari M Simeone Histology/Iummunohistochemisrty Specialist Supervisor Alternate Laboratory Supervisor Leavitt Management Group Advanced Dermatology & Cosmetic Surgery 561.819.0857 ext 100 561.819.6517 fax ksimeone@leavittmgt.com www.advancedderm.com The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message. From krista.sider <@t> utoronto.ca Fri May 10 16:13:23 2013 From: krista.sider <@t> utoronto.ca (Krista Sider) Date: Fri May 10 16:13:28 2013 Subject: [Histonet] =?windows-1252?q?Movat_Pentachrome_-_Can=92t_Remove_W?= =?windows-1252?q?oodstain_Scarlet-Acid_Fuchsin_from_Collagen?= Message-ID: Hello All, I have successfully stained porcine and mouse paraffin embedded heart tissues with Movat?s Pentachrome (MP), using EMS? MP solutions and protocol (http://www.emsdiasum.com/microscopy/technical/datasheet/26385.aspx), with some optimization on times. However, now I am working with archival human tissue that has been fixed for much longer and is much older than my other samples (Human Aorta & Aortic Valve, formalin fixed 2-6 months or 2-4 years, embedded in paraffin and stored at RT for ~6 years). I cannot seem to get the Woodstain Scarlet-Acid Fuchsin (WSAF) to pull out of the collagen with the 5% Phosphotungstic Acid (PA) or 1% Phosphomolybdic acid (PM) Acid. I have tried increasing the Bouin?s initial fixation (max 2 hrs 50*C), diluting the standard WSAF solution by 50% (1 dip) and taking the PA or PM up to 1 hr, yet there is always a lot of red still left in the collagen, in some regions as strong as the muscle (which I am sure are not muscle or dense cells). I see virtually no change with increased PA or PM time. As I have aorta in my samples I can?t just leave out the muscle stain. I would be very grateful for your insights into anything I could try to get clean collagen and stained muscle in the MP stain. Why might my method not be working? Is there something I can substitute for the WSAF that might be appropriate? Thank you very much for you help, Krista From liz <@t> premierlab.com Fri May 10 16:26:53 2013 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Fri May 10 16:26:56 2013 Subject: [Histonet] Movat Pentachrome - Can't Remove Woodstain Scarlet-Acid Fuchsin from Collagen In-Reply-To: References: Message-ID: <14E2C6176416974295479C64A11CB9AE016411AC2D2F@SBS2K8.premierlab.local> Krista Not sure if this would help but we mordant in Bouins for 1 hour at 60C, we let the bouins come up to temp (60C) before we put the slides in. We have not had good success with temps lower than 60C and if we do not warm up the bouins. We also find that fresh reagents are key in successful results, we make up our reagents, we don't use a kit. If you don't need the alcian blue portion of the stain present, my suggestion would be to try a modified trichome, - mordant in bouins and in place of the iron hematoxylin use verhoff elastic stain, differenciate and then counterstain with a regular trichrome. This will turn out really nice and it will demonstrates elastic fibers, collagen and muscle nicely. We prefer doing this over the Movats. I have a protocol if you need one. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Laboratory Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 Work (303) 682-3949 Fax (303) 682-9060 Cell (303) 881-0763 liz@premierlab.com www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Krista Sider Sent: Friday, May 10, 2013 3:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Movat Pentachrome - Can't Remove Woodstain Scarlet-Acid Fuchsin from Collagen Hello All, I have successfully stained porcine and mouse paraffin embedded heart tissues with Movat's Pentachrome (MP), using EMS' MP solutions and protocol (http://www.emsdiasum.com/microscopy/technical/datasheet/26385.aspx), with some optimization on times. However, now I am working with archival human tissue that has been fixed for much longer and is much older than my other samples (Human Aorta & Aortic Valve, formalin fixed 2-6 months or 2-4 years, embedded in paraffin and stored at RT for ~6 years). I cannot seem to get the Woodstain Scarlet-Acid Fuchsin (WSAF) to pull out of the collagen with the 5% Phosphotungstic Acid (PA) or 1% Phosphomolybdic acid (PM) Acid. I have tried increasing the Bouin's initial fixation (max 2 hrs 50*C), diluting the standard WSAF solution by 50% (1 dip) and taking the PA or PM up to 1 hr, yet there is always a lot of red still left in the collagen, in some regions as strong as the muscle (which I am sure are not muscle or dense cells). I see virtually no change with increased PA or PM time. As I have aorta in my samples I can't just leave out the muscle stain. I would be very grateful for your insights into anything I could try to get clean collagen and stained muscle in the MP stain. Why might my method not be working? Is there something I can substitute for the WSAF that might be appropriate? Thank you very much for you help, Krista _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From yliang <@t> numirabio.com Fri May 10 16:43:26 2013 From: yliang <@t> numirabio.com (Yan Liang) Date: Fri May 10 16:43:32 2013 Subject: [Histonet] ground section staining Message-ID: <8DC126BD0D840947A161BBF46AB316880355984F6A@DFW1MBX22.mex07a.mlsrvr.com> Hi Histonet, We are staining plastic bone ground section. However we have trouble on plastic ground section with H&E, Sandersons with acid fuchsin counterstaining and Goldner's Trichrome staining . It would be great appreciated if you can help or share protocol with us. Thanks you Yan .............................................. Yan Liang Ph.D. Senior Director, Histopathology From akemiat3377 <@t> yahoo.com Fri May 10 17:35:42 2013 From: akemiat3377 <@t> yahoo.com (Akemi Allison) Date: Fri May 10 17:35:47 2013 Subject: [Histonet] p40 In-Reply-To: References: Message-ID: <1368225342.75607.YahooMailNeo@web140604.mail.bf1.yahoo.com> Biocare Medical. David Tacha just gave a a workshop on this at the CSH meeting last weekend. ? Akemi Allison BS, HT(ASCP)HTL Director Phoenix Lab Consulting E-Mail: akemiat3377@yahoo.com ________________________________ From: "Taylor, Jean" To: "'ihcrg@googlegroups.com'" ; "'histonet@lists.utsouthwestern.edu'" Sent: Friday, May 10, 2013 10:53 AM Subject: [Histonet] p40 Hi everyone, I'm looking into ordering the p40 antibody for one of our pathologists. He wants to use it to help distinguish between adenocarcinoma and squamous cell ca in lung. I'm wondering what labs are using, the monoclonal or polyclonal antibody, and where you purchase it from. I haven't found that a lot of companies that carry it. Any info would be helpful. Thanks, Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wgray19 <@t> sc.rr.com Fri May 10 21:15:01 2013 From: wgray19 <@t> sc.rr.com (Wanda Shotsberger Gray) Date: Fri May 10 21:15:08 2013 Subject: [Histonet] Recording formalin "out times" Message-ID: Anyone out there using CoPath "paper free"? We are trying to use as little paper as possible, and have yet to come up with a good way/place to record the times that breast tissue comes out of formalin. If we use the "comment" line, the poor tech who scans the cassettes into CoPath must enter the time on every case, which can be way too many cases to make this feasible. We have also tried putting the out time as the name of the run, but the pathologists, who want this info in the report, can't easily see this information, and end up calling us for it. Any help would be appreciated, and vendors: Are you listening? Make this part of your LIS product, please. Thank you all, Wanda Shotsberger Gray HT/HTL (ASCP) From gu.lang <@t> gmx.at Sat May 11 03:04:21 2013 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sat May 11 03:05:10 2013 Subject: =?iso-8859-1?Q?AW:_=5BHistonet=5D_Movat_Pentachrome_-_Can't_Remove_Woodst?= =?iso-8859-1?Q?ain_Scarlet-Acid_Fuchsin_from_Collagen?= In-Reply-To: References: Message-ID: <000001ce4e1e$25ca3f80$715ebe80$@gmx.at> Try to stain first in PTA/PMA solution to impregnate the collagen fibers - perhaps testing with different times. Then follow up with red stain and the usual procedure. We use a stain called SFOG, that first impregnates 2 min with PMA and afterwards with the mixture of Chromotrop, Acidfuchsin and Anilinblue for 10 min. The longer we do the Polyacid-step the more intensive are the fibres and less intensive is the cytoplasma. I think, if after this trial the collagen is still red, that there are binding-sites in the collagen, that can't be influenced. Maybe acidfuchsin is here the main partner and a pure solution of Scarlet Red may help. Gudrun Lang -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Krista Sider Gesendet: Freitag, 10. Mai 2013 23:13 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Movat Pentachrome - Can?t Remove Woodstain Scarlet-Acid Fuchsin from Collagen Hello All, I have successfully stained porcine and mouse paraffin embedded heart tissues with Movat?s Pentachrome (MP), using EMS? MP solutions and protocol (http://www.emsdiasum.com/microscopy/technical/datasheet/26385.aspx), with some optimization on times. However, now I am working with archival human tissue that has been fixed for much longer and is much older than my other samples (Human Aorta & Aortic Valve, formalin fixed 2-6 months or 2-4 years, embedded in paraffin and stored at RT for ~6 years). I cannot seem to get the Woodstain Scarlet-Acid Fuchsin (WSAF) to pull out of the collagen with the 5% Phosphotungstic Acid (PA) or 1% Phosphomolybdic acid (PM) Acid. I have tried increasing the Bouin?s initial fixation (max 2 hrs 50*C), diluting the standard WSAF solution by 50% (1 dip) and taking the PA or PM up to 1 hr, yet there is always a lot of red still left in the collagen, in some regions as strong as the muscle (which I am sure are not muscle or dense cells). I see virtually no change with increased PA or PM time. As I have aorta in my samples I can?t just leave out the muscle stain. I would be very grateful for your insights into anything I could try to get clean collagen and stained muscle in the MP stain. Why might my method not be working? Is there something I can substitute for the WSAF that might be appropriate? Thank you very much for you help, Krista _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Sat May 11 03:09:21 2013 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sat May 11 03:10:11 2013 Subject: AW: [Histonet] Recording formalin "out times" In-Reply-To: References: Message-ID: <000101ce4e1e$d8e51400$8aaf3c00$@gmx.at> I don't know this system, but is there no possibility to record this time directly in the grossing description? Gudrun Lang -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Wanda Shotsberger Gray Gesendet: Samstag, 11. Mai 2013 04:15 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Recording formalin "out times" Anyone out there using CoPath "paper free"? We are trying to use as little paper as possible, and have yet to come up with a good way/place to record the times that breast tissue comes out of formalin. If we use the "comment" line, the poor tech who scans the cassettes into CoPath must enter the time on every case, which can be way too many cases to make this feasible. We have also tried putting the out time as the name of the run, but the pathologists, who want this info in the report, can't easily see this information, and end up calling us for it. Any help would be appreciated, and vendors: Are you listening? Make this part of your LIS product, please. Thank you all, Wanda Shotsberger Gray HT/HTL (ASCP) From Ken_Marissael <@t> vwr.com Sat May 11 13:02:32 2013 From: Ken_Marissael <@t> vwr.com (Ken_Marissael@vwr.com) Date: Sat May 11 13:02:41 2013 Subject: [Histonet] Ken Marissael is out of the office Message-ID: I will be out of the office starting 05/11/2013 and will not return until 05/20/2013. I will be moving the week of May 13th to a new residence. I will have my Blackberry, but will not have access my computer for possibly several days, so if you have an emergency contact... healthcareservice.com or customer service at 877-881-1192. From khbarr <@t> mdanderson.org Mon May 13 07:32:53 2013 From: khbarr <@t> mdanderson.org (Barr,Kaye H) Date: Mon May 13 07:32:59 2013 Subject: [Histonet] RE: Histonet Digest, Vol 114, Issue 10 In-Reply-To: <78f60e14-998c-482e-ae10-735267b26185@DCPWPEXHUBCAS01.mdanderson.edu> References: <78f60e14-998c-482e-ae10-735267b26185@DCPWPEXHUBCAS01.mdanderson.edu> Message-ID: <65C8EC869E8581459DCD566079572FAC0B7BF2C5@DCPWPEXMBX02.mdanderson.edu> The University of Texas M.D. Anderson Cancer Center is in need of a part time pathologist assistant. The hours will be late afternoon, early evening. If interested, please apply online at www.mdanderson.org, requisition number 9523. Kaye Barr, HT (ASCP) | Laboratory Manager M. D. Anderson Cancer Center | Pathology/Histology Labs 1515 Holcombe Blvd, Unit 85 | Houston, TX 77030 713.792.5366 office | khbarr@mdanderson.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Saturday, May 11, 2013 12:02 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 114, Issue 10 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Full Time Openings for Histotech and Grossing Tech (Laurie Colbert) 2. RE: Vantage and Prisma (WILLIAM DESALVO) 3. p40 (Taylor, Jean) 4. GI biopsy H&E staining (Annie T. Walton) 5. Re: GI biopsy H&E staining (Jennifer MacDonald) 6. Extra Ventana Reagent (Delray Beach Pathology Kari Simeone) 7. Movat Pentachrome - Can?t Remove Woodstain Scarlet-Acid Fuchsin from Collagen (Krista Sider) 8. RE: Movat Pentachrome - Can't Remove Woodstain Scarlet-Acid Fuchsin from Collagen (Elizabeth Chlipala) 9. ground section staining (Yan Liang) 10. Re: p40 (Akemi Allison) 11. Recording formalin "out times" (Wanda Shotsberger Gray) 12. AW: [Histonet] Movat Pentachrome - Can't Remove Woodstain Scarlet-Acid Fuchsin from Collagen (Gudrun Lang) 13. AW: [Histonet] Recording formalin "out times" (Gudrun Lang) ---------------------------------------------------------------------- Message: 1 Date: Fri, 10 May 2013 17:10:59 +0000 From: Laurie Colbert Subject: [Histonet] Full Time Openings for Histotech and Grossing Tech To: "Histonet Post (histonet@lists.utsouthwestern.edu)" Message-ID: <12ECD7346266D74691EC2BFC75285E452F2DDD4B@BFL323E10.pathmdlabs.local> Content-Type: text/plain; charset="us-ascii" PATH MD has two full time openings for a histotech and a grossing tech. Both positions require at least two years of experience and the ability to troubleshoot a variety of issues. The histotechnician shift is from 11:00pm to 7:30 am and entails embedding, cutting, and manual special stains. The grossing tech shift is 10:30 am to 7:00 pm, Monday-Friday. PATH MD is a new pathology reference lab located in West Hollywood, CA. We are rapidly expanding and are looking to add motivated and experienced personnel to our team. Salary is competitive and full medical, dental, and vision benefits are offered. If interested, please forward your resume to: lcolbert@pathmdlabs.com Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab ------------------------------ Message: 2 Date: Fri, 10 May 2013 10:18:50 -0700 From: WILLIAM DESALVO Subject: RE: [Histonet] Vantage and Prisma To: "JMaslanka@stpetes.org" , histonet Message-ID: Content-Type: text/plain; charset="iso-8859-1" I use Vantage and Sakura Prisma. The Sakura Prisma is not interfaced into Vantage. You place a bar-coded label on your H&E slides at microtomy. We scan the slides after staining, case assembly. William DeSalvo, BS HTL(ASCP) Production Manager-Anatomic Pathology Chair, NSH Quality Management Committee Owner/Consultant, Collaborative Advantage Consulting > To: histonet@lists.utsouthwestern.edu > From: JMaslanka@stpetes.org > Date: Fri, 10 May 2013 09:35:12 -0600 > Subject: [Histonet] Vantage and Prisma > > Happy Friday > Anyone out there using Ventana's Vantage with Sakura's Tissue-Tek Prisma > stainer? > > > Joe Maslanka BS, CT,HT (ASCP) > Anatomical Pathology Technical Supervisor > St Peter's Hospital,MT 59601 > (P)(406) 447-2406 > (F)(406)444-2126 > > Give thanks for ALL things..... > "Kindness is the language the blind can see & the deaf can hear- Mark > Twain > > > > This electronic mail message contains information which is confidential. > If you are not the intended recipient, please be aware that any > disclosure, photocopying, distribution or use of the contents of the > received information is prohibited. If you have received this e-mail in > error, please reply to the sender immediately and permanently delete this > message and all copies of it. Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Fri, 10 May 2013 12:53:45 -0500 From: "Taylor, Jean" Subject: [Histonet] p40 To: "'ihcrg@googlegroups.com'" , "'histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain; charset="us-ascii" Hi everyone, I'm looking into ordering the p40 antibody for one of our pathologists. He wants to use it to help distinguish between adenocarcinoma and squamous cell ca in lung. I'm wondering what labs are using, the monoclonal or polyclonal antibody, and where you purchase it from. I haven't found that a lot of companies that carry it. Any info would be helpful. Thanks, Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI ------------------------------ Message: 4 Date: Fri, 10 May 2013 11:02:30 -0700 From: "Annie T. Walton" Subject: [Histonet] GI biopsy H&E staining To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Hello all, Our Medical Director would like the goblet cells on our GI biopsies to stain a darker blue. I hand stain using the following protocol: * Xylene, 3 changes 3 minutes each * 100% Alcohol, 2 changes 1 minute each * 95% Alcohol 1 minute * Tap water rinse until water runs off evenly * Harris Hematoxylin 5 minutes * Water wash rinse until water runs clear * Clarifier 2 5 seconds * Running water 30 seconds * Bluing Solution 1 minute * Tap water rinse well * 95% Alcohol 30 seconds * Eosin Y 1 minute * 100% Alcohol, 2 changes 30 seconds * 100% Alcohol, 2 changes 1 minute each * Xylene, 3 changes 1 minute each I have recently increased the hematoxylin time from 4 to 5 minutes and decreased the time in clarifier from 25 to 5 seconds so the hematoxylin doesn't get washed out as much, but it is not good enough. I also switched from 70% alcohol to 95% before eosin and increased the time in eosin from 45 seconds to 1 minute to provide a greater contrast in hopes that that would work, but the goblet cells are still not staining dark enough. Any suggestions would greatly be appreciated! Thanks! Annie Walton, HTL (ASCP) Histology Technician Overlake Internal Medicine Associates Histology Lab 425-974-9643 awalton@oima.org ________________________________ DISCLAIMER: This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution or copying of this information is strictly prohibited. If you received this message in error, please notify the sender then delete this message. ------------------------------ Message: 5 Date: Fri, 10 May 2013 11:33:19 -0700 From: Jennifer MacDonald Subject: Re: [Histonet] GI biopsy H&E staining To: "Annie T. Walton" Cc: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=US-ASCII Use a Gill hematoxylin. Sent from my iPad On May 10, 2013, at 11:05 AM, "Annie T. Walton" wrote: > Hello all, > > Our Medical Director would like the goblet cells on our GI biopsies to stain a darker blue. I hand stain using the following protocol: > > > * Xylene, 3 changes 3 minutes each > * 100% Alcohol, 2 changes 1 minute each > * 95% Alcohol 1 minute > * Tap water rinse until water runs off evenly > * Harris Hematoxylin 5 minutes > * Water wash rinse until water runs clear > * Clarifier 2 5 seconds > * Running water 30 seconds > * Bluing Solution 1 minute > * Tap water rinse well > * 95% Alcohol 30 seconds > * Eosin Y 1 minute > * 100% Alcohol, 2 changes 30 seconds > * 100% Alcohol, 2 changes 1 minute each > * Xylene, 3 changes 1 minute each > > I have recently increased the hematoxylin time from 4 to 5 minutes and decreased the time in clarifier from 25 to 5 seconds so the hematoxylin doesn't get washed out as much, but it is not good enough. I also switched from 70% alcohol to 95% before eosin and increased the time in eosin from 45 seconds to 1 minute to provide a greater contrast in hopes that that would work, but the goblet cells are still not staining dark enough. Any suggestions would greatly be appreciated! > > Thanks! > > Annie Walton, HTL (ASCP) > Histology Technician > Overlake Internal Medicine Associates Histology Lab > 425-974-9643 > awalton@oima.org > > > ________________________________ > DISCLAIMER: This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution or copying of this information is strictly prohibited. If you received this message in error, please notify the sender then delete this message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Fri, 10 May 2013 14:46:36 -0400 From: "Delray Beach Pathology Kari Simeone" Subject: [Histonet] Extra Ventana Reagent To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi everyone. I use the Ventana NexES and when opening a new kit for PAS, I end up tossing the extra (2) Schiff's 2 (22ml each) bottles. Is there anyone out there that can use this stuff? I'd love to help someone instead of just wasting it. I inquired with Ventana and they basically told me that's just how they sell it. Yuck, wasteful. It's already registered so of no use on any other instrument but who knows?? Kari M Simeone Histology/Iummunohistochemisrty Specialist Supervisor Alternate Laboratory Supervisor Leavitt Management Group Advanced Dermatology & Cosmetic Surgery 561.819.0857 ext 100 561.819.6517 fax ksimeone@leavittmgt.com www.advancedderm.com The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message. ------------------------------ Message: 7 Date: Fri, 10 May 2013 17:13:23 -0400 From: Krista Sider Subject: [Histonet] Movat Pentachrome - Can?t Remove Woodstain Scarlet-Acid Fuchsin from Collagen To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=windows-1252 Hello All, I have successfully stained porcine and mouse paraffin embedded heart tissues with Movat?s Pentachrome (MP), using EMS? MP solutions and protocol (http://www.emsdiasum.com/microscopy/technical/datasheet/26385.aspx), with some optimization on times. However, now I am working with archival human tissue that has been fixed for much longer and is much older than my other samples (Human Aorta & Aortic Valve, formalin fixed 2-6 months or 2-4 years, embedded in paraffin and stored at RT for ~6 years). I cannot seem to get the Woodstain Scarlet-Acid Fuchsin (WSAF) to pull out of the collagen with the 5% Phosphotungstic Acid (PA) or 1% Phosphomolybdic acid (PM) Acid. I have tried increasing the Bouin?s initial fixation (max 2 hrs 50*C), diluting the standard WSAF solution by 50% (1 dip) and taking the PA or PM up to 1 hr, yet there is always a lot of red still left in the collagen, in some regions as strong as the muscle (which I am sure are not muscle or dense cells). I see virtually no change with increased PA or PM time. As I have aorta in my samples I can?t just leave out the muscle stain. I would be very grateful for your insights into anything I could try to get clean collagen and stained muscle in the MP stain. Why might my method not be working? Is there something I can substitute for the WSAF that might be appropriate? Thank you very much for you help, Krista ------------------------------ Message: 8 Date: Fri, 10 May 2013 15:26:53 -0600 From: Elizabeth Chlipala Subject: RE: [Histonet] Movat Pentachrome - Can't Remove Woodstain Scarlet-Acid Fuchsin from Collagen To: Krista Sider , "histonet@lists.utsouthwestern.edu" Message-ID: <14E2C6176416974295479C64A11CB9AE016411AC2D2F@SBS2K8.premierlab.local> Content-Type: text/plain; charset="us-ascii" Krista Not sure if this would help but we mordant in Bouins for 1 hour at 60C, we let the bouins come up to temp (60C) before we put the slides in. We have not had good success with temps lower than 60C and if we do not warm up the bouins. We also find that fresh reagents are key in successful results, we make up our reagents, we don't use a kit. If you don't need the alcian blue portion of the stain present, my suggestion would be to try a modified trichome, - mordant in bouins and in place of the iron hematoxylin use verhoff elastic stain, differenciate and then counterstain with a regular trichrome. This will turn out really nice and it will demonstrates elastic fibers, collagen and muscle nicely. We prefer doing this over the Movats. I have a protocol if you need one. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Laboratory Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 Work (303) 682-3949 Fax (303) 682-9060 Cell (303) 881-0763 liz@premierlab.com www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Krista Sider Sent: Friday, May 10, 2013 3:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Movat Pentachrome - Can't Remove Woodstain Scarlet-Acid Fuchsin from Collagen Hello All, I have successfully stained porcine and mouse paraffin embedded heart tissues with Movat's Pentachrome (MP), using EMS' MP solutions and protocol (http://www.emsdiasum.com/microscopy/technical/datasheet/26385.aspx), with some optimization on times. However, now I am working with archival human tissue that has been fixed for much longer and is much older than my other samples (Human Aorta & Aortic Valve, formalin fixed 2-6 months or 2-4 years, embedded in paraffin and stored at RT for ~6 years). I cannot seem to get the Woodstain Scarlet-Acid Fuchsin (WSAF) to pull out of the collagen with the 5% Phosphotungstic Acid (PA) or 1% Phosphomolybdic acid (PM) Acid. I have tried increasing the Bouin's initial fixation (max 2 hrs 50*C), diluting the standard WSAF solution by 50% (1 dip) and taking the PA or PM up to 1 hr, yet there is always a lot of red still left in the collagen, in some regions as strong as the muscle (which I am sure are not muscle or dense cells). I see virtually no change with increased PA or PM time. As I have aorta in my samples I can't just leave out the muscle stain. I would be very grateful for your insights into anything I could try to get clean collagen and stained muscle in the MP stain. Why might my method not be working? Is there something I can substitute for the WSAF that might be appropriate? Thank you very much for you help, Krista _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Fri, 10 May 2013 16:43:26 -0500 From: Yan Liang Subject: [Histonet] ground section staining To: "histonet@lists.utsouthwestern.edu" Message-ID: <8DC126BD0D840947A161BBF46AB316880355984F6A@DFW1MBX22.mex07a.mlsrvr.com> Content-Type: text/plain; charset="us-ascii" Hi Histonet, We are staining plastic bone ground section. However we have trouble on plastic ground section with H&E, Sandersons with acid fuchsin counterstaining and Goldner's Trichrome staining . It would be great appreciated if you can help or share protocol with us. Thanks you Yan .............................................. Yan Liang Ph.D. Senior Director, Histopathology ------------------------------ Message: 10 Date: Fri, 10 May 2013 15:35:42 -0700 (PDT) From: Akemi Allison Subject: Re: [Histonet] p40 To: "Taylor, Jean" , "'ihcrg@googlegroups.com'" , "'histonet@lists.utsouthwestern.edu'" Message-ID: <1368225342.75607.YahooMailNeo@web140604.mail.bf1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Biocare Medical. David Tacha just gave a a workshop on this at the CSH meeting last weekend. ? Akemi Allison BS, HT(ASCP)HTL Director Phoenix Lab Consulting E-Mail: akemiat3377@yahoo.com ________________________________ From: "Taylor, Jean" To: "'ihcrg@googlegroups.com'" ; "'histonet@lists.utsouthwestern.edu'" Sent: Friday, May 10, 2013 10:53 AM Subject: [Histonet] p40 Hi everyone, I'm looking into ordering the p40 antibody for one of our pathologists. He wants to use it to help distinguish between adenocarcinoma and squamous cell ca in lung. I'm wondering what labs are using, the monoclonal or polyclonal antibody, and where you purchase it from. I haven't found that a lot of companies that carry it. Any info would be helpful. Thanks, Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Fri, 10 May 2013 22:15:01 -0400 From: Wanda Shotsberger Gray Subject: [Histonet] Recording formalin "out times" To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=utf-8 Anyone out there using CoPath "paper free"? We are trying to use as little paper as possible, and have yet to come up with a good way/place to record the times that breast tissue comes out of formalin. If we use the "comment" line, the poor tech who scans the cassettes into CoPath must enter the time on every case, which can be way too many cases to make this feasible. We have also tried putting the out time as the name of the run, but the pathologists, who want this info in the report, can't easily see this information, and end up calling us for it. Any help would be appreciated, and vendors: Are you listening? Make this part of your LIS product, please. Thank you all, Wanda Shotsberger Gray HT/HTL (ASCP) ------------------------------ Message: 12 Date: Sat, 11 May 2013 10:04:21 +0200 From: "Gudrun Lang" Subject: AW: [Histonet] Movat Pentachrome - Can't Remove Woodstain Scarlet-Acid Fuchsin from Collagen To: "'Krista Sider'" Cc: histonet@lists.utsouthwestern.edu Message-ID: <000001ce4e1e$25ca3f80$715ebe80$@gmx.at> Content-Type: text/plain; charset="iso-8859-1" Try to stain first in PTA/PMA solution to impregnate the collagen fibers - perhaps testing with different times. Then follow up with red stain and the usual procedure. We use a stain called SFOG, that first impregnates 2 min with PMA and afterwards with the mixture of Chromotrop, Acidfuchsin and Anilinblue for 10 min. The longer we do the Polyacid-step the more intensive are the fibres and less intensive is the cytoplasma. I think, if after this trial the collagen is still red, that there are binding-sites in the collagen, that can't be influenced. Maybe acidfuchsin is here the main partner and a pure solution of Scarlet Red may help. Gudrun Lang -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Krista Sider Gesendet: Freitag, 10. Mai 2013 23:13 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Movat Pentachrome - Can?t Remove Woodstain Scarlet-Acid Fuchsin from Collagen Hello All, I have successfully stained porcine and mouse paraffin embedded heart tissues with Movat?s Pentachrome (MP), using EMS? MP solutions and protocol (http://www.emsdiasum.com/microscopy/technical/datasheet/26385.aspx), with some optimization on times. However, now I am working with archival human tissue that has been fixed for much longer and is much older than my other samples (Human Aorta & Aortic Valve, formalin fixed 2-6 months or 2-4 years, embedded in paraffin and stored at RT for ~6 years). I cannot seem to get the Woodstain Scarlet-Acid Fuchsin (WSAF) to pull out of the collagen with the 5% Phosphotungstic Acid (PA) or 1% Phosphomolybdic acid (PM) Acid. I have tried increasing the Bouin?s initial fixation (max 2 hrs 50*C), diluting the standard WSAF solution by 50% (1 dip) and taking the PA or PM up to 1 hr, yet there is always a lot of red still left in the collagen, in some regions as strong as the muscle (which I am sure are not muscle or dense cells). I see virtually no change with increased PA or PM time. As I have aorta in my samples I can?t just leave out the muscle stain. I would be very grateful for your insights into anything I could try to get clean collagen and stained muscle in the MP stain. Why might my method not be working? Is there something I can substitute for the WSAF that might be appropriate? Thank you very much for you help, Krista _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 13 Date: Sat, 11 May 2013 10:09:21 +0200 From: "Gudrun Lang" Subject: AW: [Histonet] Recording formalin "out times" To: "'Wanda Shotsberger Gray'" Cc: histonet@lists.utsouthwestern.edu Message-ID: <000101ce4e1e$d8e51400$8aaf3c00$@gmx.at> Content-Type: text/plain; charset="utf-8" I don't know this system, but is there no possibility to record this time directly in the grossing description? Gudrun Lang -----Urspr??ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Wanda Shotsberger Gray Gesendet: Samstag, 11. Mai 2013 04:15 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Recording formalin "out times" Anyone out there using CoPath "paper free"? We are trying to use as little paper as possible, and have yet to come up with a good way/place to record the times that breast tissue comes out of formalin. If we use the "comment" line, the poor tech who scans the cassettes into CoPath must enter the time on every case, which can be way too many cases to make this feasible. We have also tried putting the out time as the name of the run, but the pathologists, who want this info in the report, can't easily see this information, and end up calling us for it. Any help would be appreciated, and vendors: Are you listening? Make this part of your LIS product, please. Thank you all, Wanda Shotsberger Gray HT/HTL (ASCP) ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 114, Issue 10 ***************************************** From Nancy_Schmitt <@t> pa-ucl.com Mon May 13 08:22:14 2013 From: Nancy_Schmitt <@t> pa-ucl.com (Nancy Schmitt) Date: Mon May 13 08:22:23 2013 Subject: [Histonet] Recording formalin "out times" Message-ID: <906B4DA90ED1DB4DB6C7E94D7CEE6C36813C3315@PEITHA.wad.pa-ucl.com> "Total time in formalin" is included in our gross description. Nancy Schmitt Dubuque, IA ------------------------------------------------- Message: 13 Date: Sat, 11 May 2013 10:09:21 +0200 From: "Gudrun Lang" > Subject: AW: [Histonet] Recording formalin "out times" To: "'Wanda Shotsberger Gray'" > Cc: histonet@lists.utsouthwestern.edu Message-ID: <000101ce4e1e$d8e51400$8aaf3c00$@gmx.at> Content-Type: text/plain; charset="utf-8" I don't know this system, but is there no possibility to record this time directly in the grossing description? Gudrun Lang -----Urspr??ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Wanda Shotsberger Gray Gesendet: Samstag, 11. Mai 2013 04:15 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Recording formalin "out times" Anyone out there using CoPath "paper free"? We are trying to use as little paper as possible, and have yet to come up with a good way/place to record the times that breast tissue comes out of formalin. If we use the "comment" line, the poor tech who scans the cassettes into CoPath must enter the time on every case, which can be way too many cases to make this feasible. We have also tried putting the out time as the name of the run, but the pathologists, who want this info in the report, can't easily see this information, and end up calling us for it. Any help would be appreciated, and vendors: Are you listening? Make this part of your LIS product, please. Thank you all, Wanda Shotsberger Gray HT/HTL (ASCP) NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From jo-ann.bader <@t> mcgill.ca Mon May 13 13:56:03 2013 From: jo-ann.bader <@t> mcgill.ca (Jo-Ann Bader, Ms.) Date: Mon May 13 13:56:13 2013 Subject: [Histonet] Frozen bone sections Message-ID: <8C36045F0065CE48906E684F15FD4CB6320C4F4A@EXMBX2010-6.campus.MCGILL.CA> I have been asked to cut frozen mouse tibia. I have never cut frozen bone I could use some advice. Jo-Ann Bader Histology Co-Ordinator Goodman Cancer Centre McGill University 1160 Pine Ave. W - Rm 312 Montreal, QC, Canada H3G 1Y6 Tel: 514-398-8270 From lawrence.zoller <@t> unlv.edu Mon May 13 14:13:22 2013 From: lawrence.zoller <@t> unlv.edu (lawrence.zoller@unlv.edu) Date: Mon May 13 14:13:27 2013 Subject: [Histonet] Slide of the maxillary sinus Message-ID: I would very much like to obtain good views of the histology of the maxillary sinus. Our views are either cracked or have various other problems. Any really nice images would be greatly appreciated. I would like both low magnification and higher magnification views. From lbest <@t> sunriselab.com Tue May 14 06:46:48 2013 From: lbest <@t> sunriselab.com (Laurie Best) Date: Tue May 14 06:46:58 2013 Subject: [Histonet] staffing Message-ID: <68C2805BA426E24AB117C295AC50C9011411A7@NY-SML-EMAIL-01.sunriselab.com> Hi All, Does anyone have information regarding the correct ratio of Histotechs to Patholgists? I seem to remember getting that info at an NSH convention, but I cannot locate it. Thanks ahead of time. Laurie Laurie Best Histology Supervisor Sunrise Medical Laboratories 240 Miller Place Hiscksville, N.Y. 11801 tel (631)435-1515x1018 fax(631)435-2014 "This message contains privileged and confidential information intended only for the use of the addressee named above. If you are not the intended recipient of this message you must not disseminate, copy or take any action in reliance on it." From kiran_g <@t> sbcglobal.net Tue May 14 08:28:53 2013 From: kiran_g <@t> sbcglobal.net (Kiran) Date: Tue May 14 08:29:31 2013 Subject: [Histonet] Formula 83: xylene substitute Message-ID: Hello Everyone, I would like to get some feedback from Formula 83 users. Are you using it only on the stainers or for tissue processing as well. What kind of validation process is required to switch to this substitute for ihc stains? We are interested to use it for both histo and cyto lab. Any input is much appreciated. Thank you in advance, Kiran Sent from my iPad From rjbuesa <@t> yahoo.com Tue May 14 08:30:08 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue May 14 08:30:13 2013 Subject: [Histonet] staffing In-Reply-To: <68C2805BA426E24AB117C295AC50C9011411A7@NY-SML-EMAIL-01.sunriselab.com> References: <68C2805BA426E24AB117C295AC50C9011411A7@NY-SML-EMAIL-01.sunriselab.com> Message-ID: <1368538208.85061.YahooMailNeo@web163104.mail.bf1.yahoo.com> There is "no correct ratio" for HT to PT. That depends on the way the director of pathology runs the practice, the volume of work and the automation condition of the lab. You can go to http://www.histosearch.com/rene.html and read an article I wrote on staffing where you can find answer to your question. Ren? J. From: Laurie Best To: "'histonet@lists.utsouthwestern.edu'" Sent: Tuesday, May 14, 2013 7:46 AM Subject: [Histonet] staffing Hi All, Does anyone have information regarding the correct ratio of Histotechs to Patholgists? I seem to remember getting that info at an NSH convention, but I cannot locate it. Thanks ahead of time. Laurie Laurie Best Histology Supervisor Sunrise Medical Laboratories 240 Miller Place Hiscksville, N.Y. 11801 tel (631)435-1515x1018 fax(631)435-2014 "This message contains privileged and confidential information intended only for the use of the addressee named above. If you are not the intended recipient of this message you must not disseminate, copy or take any action in reliance on it." _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek <@t> digestivespecialists.com Tue May 14 09:13:39 2013 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Tue May 14 09:16:47 2013 Subject: [Histonet] Formula 83: xylene substitute In-Reply-To: References: Message-ID: <5A2BD13465E061429D6455C8D6B40E391652D4F6CE@IBMB7Exchange.digestivespecialists.com> Kiran, We use Formula 83 on both staining and processing without any problem at all. You have to watch and either rotate or change your last Formula 83s on the stain line because you can't tell if there is water in it like you can with xylene. We also use CBG's recycler to recycle Formula 83 without any problem. We have not had any problems with our IHC using Formula 83 either. Linda -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kiran Sent: Tuesday, May 14, 2013 9:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formula 83: xylene substitute Hello Everyone, I would like to get some feedback from Formula 83 users. Are you using it only on the stainers or for tissue processing as well. What kind of validation process is required to switch to this substitute for ihc stains? We are interested to use it for both histo and cyto lab. Any input is much appreciated. Thank you in advance, Kiran Sent from my iPad _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tpodawiltz <@t> lrgh.org Tue May 14 09:58:47 2013 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Tue May 14 09:58:52 2013 Subject: [Histonet] Histology Technician Opening. Message-ID: <38667E7FB77ECD4E91BFAEB8D986386325008411C5@LRGHEXVS1.practice.lrgh.org> As of May 24th I will have an opening for a Tech. Days only 40 hours per week. No weekends doing routine histology, no IHC. Located in the beautiful Lakes Region of New Hampshire. * If interested here is the link: LRGHealthcare | Career Center www.lrgh.org/Jobs Tom Podawiltz HT (ASCP) Histology Section Head/Laboratory Safety Officer. LRGHealthcare Laconia, NH 03246 603-524-3211 ext: 3220 THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From relia1 <@t> earthlink.net Tue May 14 10:27:08 2013 From: relia1 <@t> earthlink.net (Pam Barker) Date: Tue May 14 10:27:18 2013 Subject: [Histonet] RELIA Histology Careers Bulletin. 5-14-2013. Exciting New Job Opportunities. I hope you had a nice Mother's Day!! Message-ID: <00b701ce50b7$7fe03380$7fa09a80$@earthlink.net> Hi Histonetters!! I hope you had a lovely Mother's Day!!! Can you believe it? Summer is almost here!!! My phone has been ringing off the hook this past week with some exciting new opportunities. This is a quick update of the positions I am working on that I am most excited about. Some are brand new and some are additional positions where I have placed people who love their new jobs. As you know all of the positions I work with are full-time permanent positions with the best hospitals, labs and clinics. I only work with clients that offer excellent compensation including competitive salaries, great benefits and relocation assistance /sign on bonuses. Spotlight Opportunities: Mohs Tech - Phoenix, AZ -busy growing lab! Lead Histotechnologist - Covington, KY - brand new lab!!! Histology Tech - Tyler, TX - Busy Hospital Lab beautiful area!! Here is a list of the rest of the positions I am most excited to tell you about: Charlotte, NC: HT/HTL Nights 10K sign on, great benefits. Long Beach, CA: Mohs Tech with HT/HTL, Brand New Lab Chattanooga, TN: Grossing HistoTech Nights Possible lead Zanesville, OH: Histotech - Busy Lab IHC experience preferred Paterson, NJ: Histotech Nights - Brand New Lab Tyler, TX: Histotech Days- Busy Hospital Lab Syracuse, NY - Histotech Days NY lic, great lab and team to work with!! If you are interested in any of these positions please call me at 866-607-3542 or e-mail me at relia1@earthlink.net with your resume and a number where I can reach you at a time that is convenient for you. If you are interested in looking into new job opportunities in other areas that are not mentioned above please contact me as well. I work with facilities nationwide and I will keep your resume confidential. I will only represent you to jobs you tell me you are interested in looking into. Remember. It never hurts to keep an eye open even if you are happy in your present job. Also if you know anyone else that might be interested I would really appreciate it if you would pass my information along to them as well. If I place someone you refer you will earn a referral fee. Thanks-Pam Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From akemiat3377 <@t> yahoo.com Tue May 14 10:30:35 2013 From: akemiat3377 <@t> yahoo.com (Akemi Allison) Date: Tue May 14 10:30:39 2013 Subject: Fw: [Histonet] p40 In-Reply-To: <1368545343.2146.YahooMailNeo@web140603.mail.bf1.yahoo.com> References: <1368225342.75607.YahooMailNeo@web140604.mail.bf1.yahoo.com> <1368545343.2146.YahooMailNeo@web140603.mail.bf1.yahoo.com> Message-ID: <1368545435.8110.YahooMailNeo@web140606.mail.bf1.yahoo.com> Hey Jim: ? Just read the abstract you sent the link too.? Once again congratulations on yet another accepted abstract!? That was pretty much the jest of Davids' information and side-by-side images of p63 verses p40 on lung CA. ? Akemi Allison B.S., HT/HTL (ASCP) Pathology Manager Monterey Bay GI Consultants 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 (381) 375-3577? X117 Email: aallison@montereygi.com ? ________________________________ From: Jim Burchette To: Akemi Allison Cc: "Taylor, Jean" ; "ihcrg@googlegroups.com" Sent: Monday, May 13, 2013 3:44 PM Subject: Re: [Histonet] p40 Another application for p40: p40 (?Np63) and keratin 34?E12 provide greater diagnostic accuracy than p63 in the evaluation of small cell lung carcinoma in small biopsy samples. Butnor KJ, Burchette JL. Hum Pathol. 2013 Mar 22. doi:pii: S0046-8177(13)00044-0. 10.1016/j.humpath.2013.01.011. [Epub ahead of print] On Fri, May 10, 2013 at 6:35 PM, Akemi Allison wrote: Biocare Medical. David Tacha just gave a a workshop on this at the CSH meeting last weekend. >? >Akemi Allison BS, HT(ASCP)HTL >Director >Phoenix Lab Consulting >E-Mail: akemiat3377@yahoo.com > > > > >________________________________ >?From: "Taylor, Jean" >To: "'ihcrg@googlegroups.com'" ; "'histonet@lists.utsouthwestern.edu'" >Sent: Friday, May 10, 2013 10:53 AM >Subject: [Histonet] p40 > > > >Hi everyone, > >I'm looking into ordering the p40 antibody for one of our pathologists. He wants to use it to help distinguish between adenocarcinoma and squamous cell ca in lung. I'm wondering what labs are using, the monoclonal or polyclonal antibody, and where you purchase it from. I haven't found that a lot of companies that carry it. Any info would be helpful. > >Thanks, > >Jean Taylor, HT(ASCP)QIHC >IHC Tech >Meriter Health Services >Madison, WI >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Jim Burchette "Fly Fishing Bum"? <'(((>< From craigak12 <@t> gmail.com Tue May 14 10:58:43 2013 From: craigak12 <@t> gmail.com (Jb) Date: Tue May 14 10:58:47 2013 Subject: [Histonet] Lab Equipment: suggestions please Message-ID: <6A02D9DF-2B89-4696-9F78-451110EADF7C@gmail.com> Any suggestions on a good microtome? Cassette printer, slide printer? Need to purchase and would love to hear suggestions of what's good & bad products to buy. Thank you Sent from my iPhone From amber.mckenzie <@t> gastrodocs.net Tue May 14 11:28:35 2013 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Tue May 14 11:28:54 2013 Subject: [Histonet] Gross Room cleaner In-Reply-To: <6A02D9DF-2B89-4696-9F78-451110EADF7C@gmail.com> References: <6A02D9DF-2B89-4696-9F78-451110EADF7C@gmail.com> Message-ID: <5A33C952BB67F4468AF1F36D739212BCBCED66E8@JERRY.Gia.com> What do you guys use to clean your Gross Room utensils? I bought Lem'in Lift from Mantek in the past to soak the rulers, scissors, tweezers, etc...Thanks! From kiran_g <@t> sbcglobal.net Tue May 14 11:31:58 2013 From: kiran_g <@t> sbcglobal.net (Kiranjit Grewal) Date: Tue May 14 11:32:01 2013 Subject: [Histonet] Formula 83: xylene substitute In-Reply-To: <5A2BD13465E061429D6455C8D6B40E391652D4F6CE@IBMB7Exchange.digestivespecialists.com> Message-ID: <1368549118.38764.YahooMailClassic@web184404.mail.bf1.yahoo.com> Thanks, Linda. ? What about the odor? Does it have strong or irritating odor? How did you staff responded to the change? We would like to know all before trying it out. Currenly, our staff is having issues with Xylene fumes and goal is to limit any fumes or exposure to Xylene. ? I would like to hear from others who tried to use it and didn't work for them. ? -Kiran --- On Tue, 5/14/13, Blazek, Linda wrote: From: Blazek, Linda Subject: RE: [Histonet] Formula 83: xylene substitute To: "Kiran" , "histonet@lists.utsouthwestern.edu" Date: Tuesday, May 14, 2013, 7:13 AM Kiran, We use Formula 83 on both staining and processing without any problem at all.? You have to watch and either rotate or change your last Formula 83s on the stain line because you can't tell if there is water in it like you can with xylene.? We also use CBG's recycler to recycle Formula 83 without any problem.? We have not had any problems with our IHC using Formula 83 either.? Linda -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kiran Sent: Tuesday, May 14, 2013 9:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formula 83: xylene substitute Hello Everyone, I would like to get some feedback from Formula 83 users. Are you using it only on the stainers or for tissue processing as well. What kind of validation process is required to switch to this substitute for ihc stains? We are interested to use it for both histo and cyto lab. Any input is much appreciated. Thank you in advance, Kiran Sent from my iPad _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amber.mckenzie <@t> gastrodocs.net Tue May 14 11:33:01 2013 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Tue May 14 11:33:04 2013 Subject: [Histonet] Lysol IC for Ventana equipmetn cleaner In-Reply-To: <6A02D9DF-2B89-4696-9F78-451110EADF7C@gmail.com> References: <6A02D9DF-2B89-4696-9F78-451110EADF7C@gmail.com> Message-ID: <5A33C952BB67F4468AF1F36D739212BCBCED670E@JERRY.Gia.com> I used to use Amphyl to decon my Ventana equipment, but now Cardinal sells Lysol IC in its place and I was wondering how you guys make it up...water to cleaner ratio in the Vantana carboys. With Amphyl I did 20L dH20/200ml Amphyl. Thanks! From TMcNemar <@t> lmhealth.org Tue May 14 12:15:20 2013 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Tue May 14 12:14:13 2013 Subject: [Histonet] RE: Lysol IC for Ventana equipmetn cleaner In-Reply-To: <5A33C952BB67F4468AF1F36D739212BCBCED670E@JERRY.Gia.com> References: <6A02D9DF-2B89-4696-9F78-451110EADF7C@gmail.com> <5A33C952BB67F4468AF1F36D739212BCBCED670E@JERRY.Gia.com> Message-ID: We use it but we only make it up 1 gallon at a time. We use 20ml to 1 gallon of distilled per Ventana. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Tuesday, May 14, 2013 12:33 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Lysol IC for Ventana equipmetn cleaner I used to use Amphyl to decon my Ventana equipment, but now Cardinal sells Lysol IC in its place and I was wondering how you guys make it up...water to cleaner ratio in the Vantana carboys. With Amphyl I did 20L dH20/200ml Amphyl. Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From Rcartun <@t> harthosp.org Tue May 14 12:33:21 2013 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Tue May 14 12:33:30 2013 Subject: [Histonet] Re: [IHCRG] p40 In-Reply-To: References: Message-ID: <51923D21020000770003A980@gwmail3.harthosp.org> I have been very impressed with BioCare's "p40" monoclonal predilute. We are able to use it a 1:5 dilution on our Bond Max platform. Excellent immunoreactivity so far! Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> "Taylor, Jean" 5/10/2013 1:53 PM >>> Hi everyone, I?m looking into ordering the p40 antibody for one of our pathologists. He wants to use it to help distinguish between adenocarcinoma and squamous cell ca in lung. I?m wondering what labs are using, the monoclonal or polyclonal antibody, and where you purchase it from. I haven?t found that a lot of companies that carry it. Any info would be helpful. Thanks, Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI -- -- You received this message because you are subscribed to the Google Groups "ihcrg" group. The IHC Resource Group is a standing committee within the National Society for Histotechnology. To post to this group, send email to ihcrg@googlegroups.com To unsubscribe from this group, send email to ihcrg+unsubscribe@googlegroups.com For more options, visit this group at http://groups.google.com/group/ihcrg?hl=en To contact the National Society for Histotechnology, email: histo@nsh.org or call 443.535.4060. --- You received this message because you are subscribed to the Google Groups "ihcrg" group. To unsubscribe from this group and stop receiving emails from it, send an email to ihcrg+unsubscribe@googlegroups.com. For more options, visit https://groups.google.com/groups/opt_out. From sdysart <@t> mirnarx.com Tue May 14 12:39:05 2013 From: sdysart <@t> mirnarx.com (Sarah Dysart) Date: Tue May 14 12:39:12 2013 Subject: [Histonet] RE: Gross Room cleaner In-Reply-To: <5A33C952BB67F4468AF1F36D739212BCBCED66E8@JERRY.Gia.com> References: <6A02D9DF-2B89-4696-9F78-451110EADF7C@gmail.com> <5A33C952BB67F4468AF1F36D739212BCBCED66E8@JERRY.Gia.com> Message-ID: Bleach =) Just make sure to rinse after cleaning or they will rust Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Tuesday, May 14, 2013 11:29 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Gross Room cleaner What do you guys use to clean your Gross Room utensils? I bought Lem'in Lift from Mantek in the past to soak the rulers, scissors, tweezers, etc...Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Tue May 14 13:38:38 2013 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Tue May 14 13:38:48 2013 Subject: [Histonet] Re: [IHCRG] p40 In-Reply-To: <51923D21020000770003A980@gwmail3.harthosp.org> References: , <51923D21020000770003A980@gwmail3.harthosp.org> Message-ID: Richard now the ab companies are really going to hate you advocating diluting predilute's further isn't that a sacrilege, what are we going to do with you, no negs and now diluting predilutes. I am just kidding I do it all the time myself. Cheers, Patsy Date: Tue, 14 May 2013 13:33:21 -0400 From: Rcartun@harthosp.org To: ihcrg@googlegroups.com; histonet@lists.utsouthwestern.edu; jtaylor@meriter.com CC: Subject: [Histonet] Re: [IHCRG] p40 I have been very impressed with BioCare's "p40" monoclonal predilute. We are able to use it a 1:5 dilution on our Bond Max platform. Excellent immunoreactivity so far! Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> "Taylor, Jean" 5/10/2013 1:53 PM >>> Hi everyone, I?m looking into ordering the p40 antibody for one of our pathologists. He wants to use it to help distinguish between adenocarcinoma and squamous cell ca in lung. I?m wondering what labs are using, the monoclonal or polyclonal antibody, and where you purchase it from. I haven?t found that a lot of companies that carry it. Any info would be helpful. Thanks, Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI -- -- You received this message because you are subscribed to the Google Groups "ihcrg" group. The IHC Resource Group is a standing committee within the National Society for Histotechnology. To post to this group, send email to ihcrg@googlegroups.com To unsubscribe from this group, send email to ihcrg+unsubscribe@googlegroups.com For more options, visit this group at http://groups.google.com/group/ihcrg?hl=en To contact the National Society for Histotechnology, email: histo@nsh.org or call 443.535.4060. --- You received this message because you are subscribed to the Google Groups "ihcrg" group. To unsubscribe from this group and stop receiving emails from it, send an email to ihcrg+unsubscribe@googlegroups.com. For more options, visit https://groups.google.com/groups/opt_out. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jkrenz <@t> primecare.org Tue May 14 14:06:55 2013 From: jkrenz <@t> primecare.org (Renz, Jason) Date: Tue May 14 14:07:03 2013 Subject: [Histonet] Bone marrow aspirate iron stain Message-ID: <1E0E2B14C709174B8AC2BE0AE7F76833A2F451E3D7@EXCHANGE2K7.staprimecare.org> Hello, We are currently having problems with our bone marrow aspirate slides for iron stains which we run on the Ventana nexes special stainer. The RBCs look crenated or damaged. We currently fix them 5 minutes in methanol then air dry before running them on the stainer. Any suggestions? Jason Renz HT(ASCP)/lab safety officer St. Alexius Medical Center 900 East Broadway Bismarck ND, 58506 (701)530-6733 ________________________________ This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. From guadagnin.eleonora <@t> gmail.com Tue May 14 14:33:41 2013 From: guadagnin.eleonora <@t> gmail.com (Eleonora Guadagnin) Date: Tue May 14 14:33:47 2013 Subject: [Histonet] Gomori Trichrome Staining Message-ID: Hello everybody, I am performing the Gomori Trichrome staining on frozen muscle sections (10um) but I have couple of issues: 1. The green staining is TOO dark; 2. When the green is not too dark, all my muscle fibers are red. I checked the pH of the Gomori solution, is 2.15. If anyone has some advice, please!!!! Thanks a lot! Eleonora From patpxs <@t> gmail.com Tue May 14 17:35:30 2013 From: patpxs <@t> gmail.com (Paula Sicurello) Date: Tue May 14 17:35:35 2013 Subject: [Histonet] Gross Room cleaner In-Reply-To: <5A33C952BB67F4468AF1F36D739212BCBCED66E8@JERRY.Gia.com> References: <6A02D9DF-2B89-4696-9F78-451110EADF7C@gmail.com> <5A33C952BB67F4468AF1F36D739212BCBCED66E8@JERRY.Gia.com> Message-ID: Dispatch works well too. Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 On Tue, May 14, 2013 at 12:28 PM, Amber McKenzie < amber.mckenzie@gastrodocs.net> wrote: > > What do you guys use to clean your Gross Room utensils? I bought Lem'in > Lift from Mantek in the past to soak the rulers, scissors, tweezers, > etc...Thanks! > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From tony.henwood <@t> health.nsw.gov.au Tue May 14 18:01:06 2013 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Tue May 14 18:01:19 2013 Subject: [Histonet] RE: Bone marrow aspirate iron stain In-Reply-To: <1E0E2B14C709174B8AC2BE0AE7F76833A2F451E3D7@EXCHANGE2K7.staprimecare.org> References: <1E0E2B14C709174B8AC2BE0AE7F76833A2F451E3D7@EXCHANGE2K7.staprimecare.org> Message-ID: <6D6BD1DE8A5571489398B392A38A71579D25BA02@xmdb04.nch.kids> Hi Jason, I am not familiar with the stainer but is the procedure starting from water (not dewaxing & re-hydration as is used for paraffin sections)? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Renz, Jason Sent: Wednesday, 15 May 2013 5:07 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Bone marrow aspirate iron stain Hello, We are currently having problems with our bone marrow aspirate slides for iron stains which we run on the Ventana nexes special stainer. The RBCs look crenated or damaged. We currently fix them 5 minutes in methanol then air dry before running them on the stainer. Any suggestions? Jason Renz HT(ASCP)/lab safety officer St. Alexius Medical Center 900 East Broadway Bismarck ND, 58506 (701)530-6733 ________________________________ This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From LSebree <@t> uwhealth.org Tue May 14 18:24:04 2013 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Tue May 14 18:24:08 2013 Subject: [Histonet] RE: Lysol IC for Ventana equipmetn cleaner In-Reply-To: <5A33C952BB67F4468AF1F36D739212BCBCED670E@JERRY.Gia.com> References: <6A02D9DF-2B89-4696-9F78-451110EADF7C@gmail.com>, <5A33C952BB67F4468AF1F36D739212BCBCED670E@JERRY.Gia.com> Message-ID: <77DD817201982748BC67D7960F2F76AF04943E@UWHC-MBX12.uwhis.hosp.wisc.edu> The dilution is listed on the bottle...I'm not at work but its something like 1:252. Linda A. Sebree ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Amber McKenzie [amber.mckenzie@gastrodocs.net] Sent: Tuesday, May 14, 2013 11:33 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Lysol IC for Ventana equipmetn cleaner I used to use Amphyl to decon my Ventana equipment, but now Cardinal sells Lysol IC in its place and I was wondering how you guys make it up...water to cleaner ratio in the Vantana carboys. With Amphyl I did 20L dH20/200ml Amphyl. Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From turkekul <@t> gmail.com Tue May 14 19:44:32 2013 From: turkekul <@t> gmail.com (Mesru T) Date: Tue May 14 19:44:36 2013 Subject: [Histonet] HPV In situ hybridization protocol (FISH or CISH) Message-ID: Dear Histonetters, Does anybody have a protocol for HPV in situ hybridization (preferably high risk type probes). Preferably on ThinPrep cytological samples or similar. Regards, Mesru Turkekul From rook570 <@t> comcast.net Tue May 14 20:10:43 2013 From: rook570 <@t> comcast.net (rook570@comcast.net) Date: Tue May 14 20:11:16 2013 Subject: [Histonet] Mohs histology hourly rate In-Reply-To: <226820211.824341.1368580058298.JavaMail.root@sz0141a.westchester.pa.mail.comcast.net> Message-ID: <1558675134.824441.1368580243505.JavaMail.root@sz0141a.westchester.pa.mail.comcast.net> Hello, I was wondering if anyone knows what the going rate is for a Mohs histologist (Hourly). I have 12 years experience as a histologist doing routine histology and just recently got a job doing Mohs histology. Thanks in advance for any feed back, Christina From chapcl <@t> yahoo.com Tue May 14 20:12:24 2013 From: chapcl <@t> yahoo.com (Will Chappell) Date: Tue May 14 20:12:29 2013 Subject: [Histonet] Mohs histology hourly rate In-Reply-To: <1558675134.824441.1368580243505.JavaMail.root@sz0141a.westchester.pa.mail.comcast.net> References: <1558675134.824441.1368580243505.JavaMail.root@sz0141a.westchester.pa.mail.comcast.net> Message-ID: Location matters. Where? Sent from my iPhone On May 14, 2013, at 6:10 PM, rook570@comcast.net wrote: > Hello, I was wondering if anyone knows what the going rate is for a Mohs histologist (Hourly). I have 12 years experience as a histologist doing routine histology and just recently got a job doing Mohs histology. Thanks in advance for any feed back, Christina > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From max_histo_00 <@t> yahoo.it Wed May 15 01:10:41 2013 From: max_histo_00 <@t> yahoo.it (Massimo) Date: Wed May 15 01:10:50 2013 Subject: [Histonet] Gomori Trichrome Staining In-Reply-To: References: Message-ID: <1368598241.28268.YahooMailNeo@web172001.mail.ir2.yahoo.com> Hi, you can try, if you do not already, to wash?briefly? in a solution of 0.2 ml. of?glacial acetic acid in 100 ml. of?distilled water, before the?final dehydration. That makes the colors more delicate and transparent. The Gomori's trichromic results are similar to those obtained? with the Masson's trichrome staining: Cytoplasms from bright red to greenish. Kind Regards Massimo Tosi "A humble Chemical Engineer who loves Histology" ________________________________ Da: Eleonora Guadagnin A: histonet@lists.utsouthwestern.edu Inviato: Marted? 14 Maggio 2013 21:33 Oggetto: [Histonet] Gomori Trichrome Staining Hello everybody, I am performing the Gomori Trichrome staining on frozen muscle sections (10um) but I have couple of issues: 1. The green staining is TOO dark; 2. When the green is not too dark, all my muscle fibers are red. I checked the pH of the Gomori solution, is 2.15. If anyone has some advice, please!!!! Thanks a lot! Eleonora _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pitts.jaclyn <@t> gmail.com Wed May 15 07:16:33 2013 From: pitts.jaclyn <@t> gmail.com (Jaclyn Pitts) Date: Wed May 15 07:16:40 2013 Subject: [Histonet] Mohs histology hourly rate In-Reply-To: <1558675134.824441.1368580243505.JavaMail.root@sz0141a.westchester.pa.mail.comcast.net> References: <226820211.824341.1368580058298.JavaMail.root@sz0141a.westchester.pa.mail.comcast.net> <1558675134.824441.1368580243505.JavaMail.root@sz0141a.westchester.pa.mail.comcast.net> Message-ID: Good Question. I am interested in this too, as I wil lbe starting to do MOHS soon. I am located in Minnesota. On Tue, May 14, 2013 at 8:10 PM, wrote: > Hello, I was wondering if anyone knows what the going rate is for a Mohs > histologist (Hourly). I have 12 years experience as a histologist doing > routine histology and just recently got a job doing Mohs histology. Thanks > in advance for any feed back, Christina > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- *Jaclyn Pitts, HT(ASCP)CM* 218-454-3520 Dermatology Professionals, PA 15167 Edgewood Dr. Suite 200 Baxter, MN 56425 From chapcl <@t> yahoo.com Wed May 15 08:15:26 2013 From: chapcl <@t> yahoo.com (Will Chappell) Date: Wed May 15 08:15:32 2013 Subject: [Histonet] Mohs histology hourly rate In-Reply-To: References: <226820211.824341.1368580058298.JavaMail.root@sz0141a.westchester.pa.mail.comcast.net> <1558675134.824441.1368580243505.JavaMail.root@sz0141a.westchester.pa.mail.comcast.net> Message-ID: <908CCA1B-5C0B-4B34-8C1B-8FE7BF5E975A@yahoo.com> I would expect around $25 in twin cities and between $20 and $23 outside. For MOHs with 10-15 years experience. You could find higher wages, but those would be few and far between. Sent from my iPhone On May 15, 2013, at 5:16 AM, Jaclyn Pitts wrote: > Good Question. I am interested in this too, as I wil lbe starting to do > MOHS soon. I am located in Minnesota. > > > On Tue, May 14, 2013 at 8:10 PM, wrote: > >> Hello, I was wondering if anyone knows what the going rate is for a Mohs >> histologist (Hourly). I have 12 years experience as a histologist doing >> routine histology and just recently got a job doing Mohs histology. Thanks >> in advance for any feed back, Christina >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > -- > > *Jaclyn Pitts, HT(ASCP)CM* > > 218-454-3520 > > Dermatology Professionals, PA > > 15167 Edgewood Dr. Suite 200 > > Baxter, MN 56425 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nguy0515 <@t> gmail.com Wed May 15 14:01:22 2013 From: nguy0515 <@t> gmail.com (Trini Nguyen) Date: Wed May 15 14:01:28 2013 Subject: [Histonet] Re: Mohs histology hourly rate Message-ID: >From my experience, if your new to mohs and located in the midwest (Minnesota) the typical starting wage is between $18.50-$20. From TJohnson <@t> gnf.org Wed May 15 16:46:45 2013 From: TJohnson <@t> gnf.org (Teri Johnson) Date: Wed May 15 16:46:52 2013 Subject: [Histonet] Ventana fluorescent IHC users Message-ID: <9F3CFEE76E51B64991C7485270890B40497727E4@EX4.lj.gnf.org> Dear colleagues, If you are doing Immunofluorescence on the Ventana instruments (Alexafluor labeling), please contact me. Bonus points if you are doing them on the Discovery XT. Teri Johnson Manager, Histology GNF - San Diego, CA 858-332-4752 From naveedafahim <@t> yahoo.ca Wed May 15 17:13:15 2013 From: naveedafahim <@t> yahoo.ca (Naveedafahim) Date: Wed May 15 17:13:41 2013 Subject: [Histonet] Re: Histonet Digest, Vol 114, Issue 14 Message-ID: Hi Eleanora We do trichrome on muscle but ph of solution we keep at 3.4 and first hematoxylin then trichrome for 10 minutes and then wash in water 2-3 times and then two changes of 95 percent alcohol then DCM Naveeda On 2013-05-15, at 1:02 PM, histonet-request@lists.utsouthwestern.edu wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. RE: Lysol IC for Ventana equipmetn cleaner (Tom McNemar) > 2. Re: [IHCRG] p40 (Richard Cartun) > 3. RE: Gross Room cleaner (Sarah Dysart) > 4. RE: Re: [IHCRG] p40 (Patsy Ruegg) > 5. Bone marrow aspirate iron stain (Renz, Jason) > 6. Gomori Trichrome Staining (Eleonora Guadagnin) > 7. Re: Gross Room cleaner (Paula Sicurello) > 8. RE: Bone marrow aspirate iron stain (Tony Henwood (SCHN)) > 9. RE: Lysol IC for Ventana equipmetn cleaner (Sebree Linda A) > 10. HPV In situ hybridization protocol (FISH or CISH) (Mesru T) > 11. Mohs histology hourly rate (rook570@comcast.net) > 12. Re: Mohs histology hourly rate (Will Chappell) > 13. Re: Gomori Trichrome Staining (Massimo) > 14. Re: Mohs histology hourly rate (Jaclyn Pitts) > 15. Re: Mohs histology hourly rate (Will Chappell) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Tue, 14 May 2013 13:15:20 -0400 > From: Tom McNemar > Subject: [Histonet] RE: Lysol IC for Ventana equipmetn cleaner > To: 'Amber McKenzie' , > "Histonet@lists.utsouthwestern.edu" > > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > We use it but we only make it up 1 gallon at a time. We use 20ml to 1 gallon of distilled per Ventana. > > Tom McNemar, HT(ASCP) > Histology Co-ordinator > Licking Memorial Health Systems > (740) 348-4163 > (740) 348-4166 > tmcnemar@lmhealth.org > www.LMHealth.org > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie > Sent: Tuesday, May 14, 2013 12:33 PM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] Lysol IC for Ventana equipmetn cleaner > > > I used to use Amphyl to decon my Ventana equipment, but now Cardinal sells Lysol IC in its place and I was wondering how you guys make it up...water to cleaner ratio in the Vantana carboys. With Amphyl I did 20L dH20/200ml Amphyl. Thanks! > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. > > > > ------------------------------ > > Message: 2 > Date: Tue, 14 May 2013 13:33:21 -0400 > From: Richard Cartun > Subject: [Histonet] Re: [IHCRG] p40 > To: "'ihcrg@googlegroups.com'" , > "'histonet@lists.utsouthwestern.edu'" > , Jean Taylor > > Message-ID: <51923D21020000770003A980@gwmail3.harthosp.org> > Content-Type: text/plain; charset="utf-8" > > I have been very impressed with BioCare's "p40" monoclonal predilute. > We are able to use it a 1:5 dilution on our Bond Max platform. > Excellent immunoreactivity so far! > > Richard > > > Richard W. Cartun, MS, PhD > Director, Histology & Immunopathology > Director, Biospecimen Collection Programs > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 545-1596 Office > (860) 545-2204 Fax > > >>>> "Taylor, Jean" 5/10/2013 1:53 PM >>> > > Hi everyone, > > I???m looking into ordering the p40 antibody for one of our pathologists. > He wants to use it to help distinguish between adenocarcinoma and > squamous cell ca in lung. I???m wondering what labs are using, the > monoclonal or polyclonal antibody, and where you purchase it from. I > haven???t found that a lot of companies that carry it. Any info would be > helpful. > > Thanks, > > Jean Taylor, HT(ASCP)QIHC > IHC Tech > Meriter Health Services > Madison, WI > > -- > -- > You received this message because you are subscribed to the Google > Groups "ihcrg" group. The IHC Resource Group is a standing committee > within the National Society for Histotechnology. > > To post to this group, send email to ihcrg@googlegroups.com > To unsubscribe from this group, send email to > ihcrg+unsubscribe@googlegroups.com > For more options, visit this group at > http://groups.google.com/group/ihcrg?hl=en > > To contact the National Society for Histotechnology, email: > histo@nsh.org or call 443.535.4060. > > --- > You received this message because you are subscribed to the Google > Groups "ihcrg" group. > To unsubscribe from this group and stop receiving emails from it, send > an email to ihcrg+unsubscribe@googlegroups.com. > For more options, visit https://groups.google.com/groups/opt_out. > > > > ------------------------------ > > Message: 3 > Date: Tue, 14 May 2013 17:39:05 +0000 > From: Sarah Dysart > Subject: [Histonet] RE: Gross Room cleaner > To: Amber McKenzie , > "Histonet@lists.utsouthwestern.edu" > > Message-ID: > > > Content-Type: text/plain; charset="us-ascii" > > Bleach =) > Just make sure to rinse after cleaning or they will rust > > Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) > Histotechnologist > Mirna Therapeutics > 2150 Woodward Street > Suite 100 > Austin, Texas 78744 > (512)901-0900 ext. 6912 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie > Sent: Tuesday, May 14, 2013 11:29 AM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] Gross Room cleaner > > > What do you guys use to clean your Gross Room utensils? I bought Lem'in Lift from Mantek in the past to soak the rulers, scissors, tweezers, etc...Thanks! > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 4 > Date: Tue, 14 May 2013 12:38:38 -0600 > From: Patsy Ruegg > Subject: RE: [Histonet] Re: [IHCRG] p40 > To: Richard Cartun , ihcrg ihcrg > , "Histonet@Lists. Edu" > , jean taylor > Message-ID: > Content-Type: text/plain; charset="Windows-1252" > > Richard now the ab companies are really going to hate you advocating diluting predilute's further isn't that a sacrilege, what are we going to do with you, no negs and now diluting predilutes. I am just kidding I do it all the time myself. > Cheers, Patsy > > Date: Tue, 14 May 2013 13:33:21 -0400 > From: Rcartun@harthosp.org > To: ihcrg@googlegroups.com; histonet@lists.utsouthwestern.edu; jtaylor@meriter.com > CC: > Subject: [Histonet] Re: [IHCRG] p40 > > I have been very impressed with BioCare's "p40" monoclonal predilute. > We are able to use it a 1:5 dilution on our Bond Max platform. > Excellent immunoreactivity so far! > > Richard > > > Richard W. Cartun, MS, PhD > Director, Histology & Immunopathology > Director, Biospecimen Collection Programs > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 545-1596 Office > (860) 545-2204 Fax > > >>>> "Taylor, Jean" 5/10/2013 1:53 PM >>> > > Hi everyone, > > I?m looking into ordering the p40 antibody for one of our pathologists. > He wants to use it to help distinguish between adenocarcinoma and > squamous cell ca in lung. I?m wondering what labs are using, the > monoclonal or polyclonal antibody, and where you purchase it from. I > haven?t found that a lot of companies that carry it. Any info would be > helpful. > > Thanks, > > Jean Taylor, HT(ASCP)QIHC > IHC Tech > Meriter Health Services > Madison, WI > > -- > -- > You received this message because you are subscribed to the Google > Groups "ihcrg" group. The IHC Resource Group is a standing committee > within the National Society for Histotechnology. > > To post to this group, send email to ihcrg@googlegroups.com > To unsubscribe from this group, send email to > ihcrg+unsubscribe@googlegroups.com > For more options, visit this group at > http://groups.google.com/group/ihcrg?hl=en > > To contact the National Society for Histotechnology, email: > histo@nsh.org or call 443.535.4060. > > --- > You received this message because you are subscribed to the Google > Groups "ihcrg" group. > To unsubscribe from this group and stop receiving emails from it, send > an email to ihcrg+unsubscribe@googlegroups.com. > For more options, visit https://groups.google.com/groups/opt_out. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > Message: 5 > Date: Tue, 14 May 2013 14:06:55 -0500 > From: "Renz, Jason" > Subject: [Histonet] Bone marrow aspirate iron stain > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > <1E0E2B14C709174B8AC2BE0AE7F76833A2F451E3D7@EXCHANGE2K7.staprimecare.org> > > Content-Type: text/plain; charset="iso-8859-1" > > Hello, We are currently having problems with our bone marrow aspirate slides for iron stains which we run on the Ventana nexes special stainer. The RBCs look crenated or damaged. We currently fix them 5 minutes in methanol then air dry before running them on the stainer. Any suggestions? > Jason Renz > HT(ASCP)/lab safety officer > St. Alexius Medical Center > 900 East Broadway > Bismarck ND, 58506 > (701)530-6733 > > ________________________________ > This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. > > > > ------------------------------ > > Message: 6 > Date: Tue, 14 May 2013 15:33:41 -0400 > From: Eleonora Guadagnin > Subject: [Histonet] Gomori Trichrome Staining > To: histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset=ISO-8859-1 > > Hello everybody, > I am performing the Gomori Trichrome staining on frozen muscle sections > (10um) but I have couple of issues: > 1. The green staining is TOO dark; > 2. When the green is not too dark, all my muscle fibers are red. > > I checked the pH of the Gomori solution, is 2.15. > > If anyone has some advice, please!!!! > Thanks a lot! > > Eleonora > > > ------------------------------ > > Message: 7 > Date: Tue, 14 May 2013 18:35:30 -0400 > From: Paula Sicurello > Subject: Re: [Histonet] Gross Room cleaner > To: Amber McKenzie > Cc: "Histonet@lists.utsouthwestern.edu" > > Message-ID: > > Content-Type: text/plain; charset=ISO-8859-1 > > Dispatch works well too. > > Paula > -- > Paula Sicurello, HTL (ASCP) > Supervisor, Clinical Electron Microscopy Laboratory > Duke University Health System > Rm.#251M, Duke South, Green Zone > Durham, North Carolina 27710 > P: 919.684.2091 > > On Tue, May 14, 2013 at 12:28 PM, Amber McKenzie < > amber.mckenzie@gastrodocs.net> wrote: > >> >> What do you guys use to clean your Gross Room utensils? I bought Lem'in >> Lift from Mantek in the past to soak the rulers, scissors, tweezers, >> etc...Thanks! >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Message: 8 > Date: Tue, 14 May 2013 23:01:06 +0000 > From: "Tony Henwood (SCHN)" > Subject: [Histonet] RE: Bone marrow aspirate iron stain > To: "'Renz, Jason'" , > "histonet@lists.utsouthwestern.edu" > > Message-ID: <6D6BD1DE8A5571489398B392A38A71579D25BA02@xmdb04.nch.kids> > Content-Type: text/plain; charset="us-ascii" > > Hi Jason, > I am not familiar with the stainer but is the procedure starting from water (not dewaxing & re-hydration as is used for paraffin sections)? > > Regards > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) > Laboratory Manager & Senior Scientist > Tel: 612 9845 3306 > Fax: 612 9845 3318 > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead > Locked Bag 4001, Westmead NSW 2145, AUSTRALIA > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Renz, Jason > Sent: Wednesday, 15 May 2013 5:07 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Bone marrow aspirate iron stain > > Hello, We are currently having problems with our bone marrow aspirate slides for iron stains which we run on the Ventana nexes special stainer. The RBCs look crenated or damaged. We currently fix them 5 minutes in methanol then air dry before running them on the stainer. Any suggestions? > Jason Renz > HT(ASCP)/lab safety officer > St. Alexius Medical Center > 900 East Broadway > Bismarck ND, 58506 > (701)530-6733 > > ________________________________ > This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ********************************************************************************* > This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. > > Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. > > This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. > ********************************************************************************* > > > > ------------------------------ > > Message: 9 > Date: Tue, 14 May 2013 23:24:04 +0000 > From: Sebree Linda A > Subject: [Histonet] RE: Lysol IC for Ventana equipmetn cleaner > To: Amber McKenzie , > "Histonet@lists.utsouthwestern.edu" > > Message-ID: > <77DD817201982748BC67D7960F2F76AF04943E@UWHC-MBX12.uwhis.hosp.wisc.edu> > > Content-Type: text/plain; charset="us-ascii" > > The dilution is listed on the From Sherrian.McAnn <@t> va.gov Wed May 15 18:44:52 2013 From: Sherrian.McAnn <@t> va.gov (McAnn, Sherrian) Date: Wed May 15 18:45:38 2013 Subject: [Histonet] Mohs histology hourly rate In-Reply-To: <1558675134.824441.1368580243505.JavaMail.root@sz0141a.westchester.pa.mail.comcast.net> References: <226820211.824341.1368580058298.JavaMail.root@sz0141a.westchester.pa.mail.comcast.net> <1558675134.824441.1368580243505.JavaMail.root@sz0141a.westchester.pa.mail.comcast.net> Message-ID: <61E2B58CECEF384094A363989D47C09009CC9079@VHAV17MSGA2.v17.med.va.gov> Here in San Antonio, Texas..I hear it is a lot more than that . Maybe it is a supply and demand thing.. starting wages for a bench Histology Tech here can run between 19.00 and 21.00 dollars. I have heard that it is a lot more for a MOHS tech..like maybe around 30.00 dollars and up. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of rook570@comcast.net Sent: Tuesday, May 14, 2013 8:11 PM To: . Subject: [Histonet] Mohs histology hourly rate Hello, I was wondering if anyone knows what the going rate is for a Mohs histologist (Hourly). I have 12 years experience as a histologist doing routine histology and just recently got a job doing Mohs histology. Thanks in advance for any feed back, Christina _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From michaela.tourville <@t> duke.edu Thu May 16 05:39:36 2013 From: michaela.tourville <@t> duke.edu (Michaela Lefaivre) Date: Thu May 16 05:39:51 2013 Subject: [Histonet] hsp70 Message-ID: Hello I'm new to this but here goes... Does anyone have HSP70 worked up and if so what is your methodology? I am trying to work it up on hepatacellular carcinoma using Santa Cruz antibody. Thanks for any input. Michaela LeFaivre BS, HTL (ASCP) CM Molecular Technician III Molecular Pathology Rm 4344 Purple Zone 919-684-4303 HIPAA Privacy Notification: This message and any accompanying documents are covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, and contain information intended for the specific individual (s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying or the taking of any action based on the contents of this information is strictly prohibited . If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From madelinegi <@t> yahoo.com Thu May 16 06:57:51 2013 From: madelinegi <@t> yahoo.com (Madeline Gi) Date: Thu May 16 06:57:56 2013 Subject: [Histonet] PAS D Message-ID: <1368705471.19185.YahooMailClassic@web163901.mail.gq1.yahoo.com> Hello eveyone?I was just wondering who does PAS D and would you please share your procedure with me, it been a while since I ve done this stain. Thank you in advance ? Madeline Rotger Milanese H.T. BSHCS 500 New Hempstead Rd. New City N.Y. 10965 845-362-3200 Ext 129 madelinegi@yahoo.com From CDavis <@t> che-east.org Thu May 16 07:51:00 2013 From: CDavis <@t> che-east.org (Davis, Cassie) Date: Thu May 16 07:55:17 2013 Subject: [Histonet] bone marrow aspirate slides for iron stains In-Reply-To: <7a3a909a-1842-46d3-97c8-59aa0814748e@CHESXH02.one.ads.che.org> References: <7a3a909a-1842-46d3-97c8-59aa0814748e@CHESXH02.one.ads.che.org> Message-ID: <08861B9CF6C7774E874635A4818AE37B07A3C6F0@CHEXCMS01.one.ads.che.org> Try hydrating the slide in 95% then distilled before staining. The iron stain is made in water so hydrating to water should help the stain transfer better. Hope this helps :) Cassandra Davis CDavis@che-east.org 302-575-8095 Saint Francis Hospital Saintfrancishealthcare.org Saint Francis Facebook Page Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From wrglo5 <@t> aol.com Thu May 16 11:00:38 2013 From: wrglo5 <@t> aol.com (wrglo5@aol.com) Date: Thu May 16 11:00:41 2013 Subject: [Histonet] fungal stain for frozen sections Message-ID: <8D02065A43D1586-12D0-270D5@webmail-m140.sysops.aol.com> Hi, I work in a Surgical Pathology frozen section lab outside the OR rooms. Normally we only perform an H&E on the frozens. Now we are looking for a fast and inexpensive stain for fungus on our frozen sections. Fast is the operative word because we have to report diagnoses back to ORs in a 20 minute timeperiod. Any ideas to help? Thanks! From chapcl <@t> yahoo.com Thu May 16 11:16:45 2013 From: chapcl <@t> yahoo.com (Will Chappell) Date: Thu May 16 11:16:49 2013 Subject: [Histonet] fungal stain for frozen sections In-Reply-To: <8D02065A43D1586-12D0-270D5@webmail-m140.sysops.aol.com> References: <8D02065A43D1586-12D0-270D5@webmail-m140.sysops.aol.com> Message-ID: <07CCEDF2-8921-46E1-A20E-4E576B177C36@yahoo.com> It appears, from a research paper I was reading, that a 1% solution of uvitex 2b added to eosin will cause fungus to fluoresce under uv light. I haven't performed it, however it could be as quick as an H&E stain. Looks promising. Http://rd.springer.com/article/10.1007%2FBF02889994#page-1 Will Chappell CHOC children's hospital. Sent from my iPhone On May 16, 2013, at 9:00 AM, wrglo5@aol.com wrote: > Hi, I work in a Surgical Pathology frozen section lab outside the OR rooms. Normally we only perform an H&E on the frozens. Now we are looking for a fast and inexpensive stain for fungus on our frozen sections. Fast is the operative word because we have to report diagnoses back to ORs in a 20 minute timeperiod. Any ideas to help? Thanks! > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From robert_schoonhoven <@t> yahoo.com Thu May 16 14:23:27 2013 From: robert_schoonhoven <@t> yahoo.com (robert_schoonhoven) Date: Thu May 16 14:23:34 2013 Subject: [Histonet] fungal stain for frozen sections Message-ID: <51489.37755.bm@smtp115.mail.bf1.yahoo.com>

At what wave length?  Eosin  is is also fluorescent 

 

Sent from my Boost Mobile phone.

 

 

 

------ Original Message ------
From: Will Chappells
Date: 5/16/2013 12:18 PM
To: wrglo5@aol.com;
Cc: histonet@lists.utsouthwestern.edu;
Subject: Re: [Histonet] fungal stain for frozen sections

 

It appears, from a research paper I was reading, that a 1% solution of uvitex 2b added to eosin will cause fungus to fluoresce under uv light. 

I haven't performed it, however it could be as quick as an H&E stain. Looks promising. 

Http://rd.springer.com/article/10.1007%2FBF02889994#page-1

Will Chappell
CHOC children's hospital. 

Sent from my iPhone

On May 16, 2013, at 9:00 AM, wrglo5@aol.com wrote:

> Hi, I work in a Surgical Pathology frozen section lab outside the OR rooms. Normally we only perform an H&E on the frozens. Now we are looking for a fast and inexpensive stain for fungus on our frozen sections. Fast is the operative word because we have to report diagnoses back to ORs in a 20 minute timeperiod. Any ideas to help? Thanks!
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From amosbrooks <@t> gmail.com Thu May 16 15:39:33 2013 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Thu May 16 15:39:38 2013 Subject: [Histonet] HSP70 Message-ID: Hi, We had decent luck with the abCam antibody ab5542 at 1:1600 with no retrieval. Good luck, Amos From mjdessoye <@t> commonwealthhealth.net Thu May 16 15:49:12 2013 From: mjdessoye <@t> commonwealthhealth.net (Dessoye, Michael J) Date: Thu May 16 15:49:23 2013 Subject: [Histonet] IHC on non-charged slides Message-ID: I'm hoping Histonet can come through for me! I have an unusual case that I need to run IHC on. Antibody is Pan Keratin Cocktail from Cell Marque with recommended protocol on a Benchmark Ultra with i-view detection. Only problem is, I only have two slides and they are not charged. The tissues have been air-dried but not baked. I cannot obtain more slides. I'm wondering if there's any kind of pre-treatment I could try to try to help the tissue stay on. We have run slides like this in the past with mixed results. Most of the time, the tissue washes off. Any tips or tricks that might help out in this situation? Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdessoye@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Commonwealth Health. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. From rjbuesa <@t> yahoo.com Thu May 16 15:53:55 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu May 16 15:54:01 2013 Subject: [Histonet] IHC on non-charged slides In-Reply-To: References: Message-ID: <1368737635.83378.YahooMailNeo@web163106.mail.bf1.yahoo.com> After the sections are on the slides there is little (is anything) you can do to make them more resistant to the IHC protocol. My suggestions is to perform the procedure manually and instead of HIER use enzymatic retrieval that is less "traumatic" to the sections. Ren? J. From: "Dessoye, Michael J" To: histonet@lists.utsouthwestern.edu Sent: Thursday, May 16, 2013 4:49 PM Subject: [Histonet] IHC on non-charged slides I'm hoping Histonet can come through for me!? I have an unusual case that I need to run IHC on.? Antibody is Pan Keratin Cocktail from Cell Marque with recommended protocol on a Benchmark Ultra with i-view detection.? Only problem is, I only have two slides and they are not charged.? The tissues have been air-dried but not baked.? I cannot obtain more slides. I'm wondering if there's any kind of pre-treatment I could try to try to help the tissue stay on.? We have run slides like this in the past with mixed results.? Most of the time, the tissue washes off. Any tips or tricks that might help out in this situation? Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdessoye@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Commonwealth Health. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DSiena <@t> statlab.com Thu May 16 16:00:16 2013 From: DSiena <@t> statlab.com (Debra Siena) Date: Thu May 16 16:00:34 2013 Subject: [Histonet] IHC on non-charged slides In-Reply-To: References: Message-ID: <2258F311-A694-43A4-A952-01BEF84A3A4E@statlab.com> Michael There is a procedure where you can transfer the sections to a charges slide with a product called mount quick. You can check with newcomer supply they sell it. I know of several labs that have used it on these occasions and it works well. Sent from my iPhone On May 16, 2013, at 4:50 PM, "Dessoye, Michael J" wrote: > I'm hoping Histonet can come through for me! I have an unusual case that I need to run IHC on. Antibody is Pan Keratin Cocktail from Cell Marque with recommended protocol on a Benchmark Ultra with i-view detection. Only problem is, I only have two slides and they are not charged. The tissues have been air-dried but not baked. I cannot obtain more slides. > > I'm wondering if there's any kind of pre-treatment I could try to try to help the tissue stay on. We have run slides like this in the past with mixed results. Most of the time, the tissue washes off. > > Any tips or tricks that might help out in this situation? > > > > Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdessoye@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 > _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ > > This email and any files transmitted with it are confidential and > intended solely for the use of the individual or entity to whom > they are addressed. > If you have received this email in error please notify the > originator of the message. This footer also confirms that this > email message has been scanned for the presence of computer viruses. > > Any views expressed in this message are those of the individual > sender, except where the sender specifies and with authority, > states them to be the views of Commonwealth Health. > > Scanning of this message and addition of this footer is performed > by Websense Email Security software in conjunction with > virus detection software. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From marktarango <@t> gmail.com Thu May 16 16:12:06 2013 From: marktarango <@t> gmail.com (Mark Tarango) Date: Thu May 16 16:12:11 2013 Subject: [Histonet] IHC on non-charged slides In-Reply-To: <2258F311-A694-43A4-A952-01BEF84A3A4E@statlab.com> References: <2258F311-A694-43A4-A952-01BEF84A3A4E@statlab.com> Message-ID: Yes, if the stain can wait get some mount quick from Newcomer. There is a good chance the tissue will fall off if you proceed with the tissue on regular slides. I would wait until I get the mount quick. Everyone else reading this should order a tube now for emergencies. You will find yourself in this position at some point and if you have the stuff on hand, it's no problem. I just used it today to transfer cells from a cytocentrifuge slide to a charged slide for FISH. It saved the patient a re-biopsy. Mark On Thu, May 16, 2013 at 2:00 PM, Debra Siena wrote: > Michael > > There is a procedure where you can transfer the sections to a charges > slide with a product called mount quick. You can check with newcomer supply > they sell it. I know of several labs that have used it on these occasions > and it works well. > > Sent from my iPhone > > On May 16, 2013, at 4:50 PM, "Dessoye, Michael J" < > mjdessoye@commonwealthhealth.net> wrote: > > > I'm hoping Histonet can come through for me! I have an unusual case > that I need to run IHC on. Antibody is Pan Keratin Cocktail from Cell > Marque with recommended protocol on a Benchmark Ultra with i-view > detection. Only problem is, I only have two slides and they are not > charged. The tissues have been air-dried but not baked. I cannot obtain > more slides. > > > > I'm wondering if there's any kind of pre-treatment I could try to try to > help the tissue stay on. We have run slides like this in the past with > mixed results. Most of the time, the tissue washes off. > > > > Any tips or tricks that might help out in this situation? > > > > > > > > Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General > Hospital | An Affiliate of Commonwealth Health | > mjdessoye@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA > 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 > > _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ > _ _ > > > > This email and any files transmitted with it are confidential and > > intended solely for the use of the individual or entity to whom > > they are addressed. > > If you have received this email in error please notify the > > originator of the message. This footer also confirms that this > > email message has been scanned for the presence of computer viruses. > > > > Any views expressed in this message are those of the individual > > sender, except where the sender specifies and with authority, > > states them to be the views of Commonwealth Health. > > > > Scanning of this message and addition of this footer is performed > > by Websense Email Security software in conjunction with > > virus detection software. > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From tony.henwood <@t> health.nsw.gov.au Thu May 16 19:31:05 2013 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Thu May 16 19:31:38 2013 Subject: [Histonet] fungal stain for frozen sections In-Reply-To: <8D02065A43D1586-12D0-270D5@webmail-m140.sysops.aol.com> References: <8D02065A43D1586-12D0-270D5@webmail-m140.sysops.aol.com> Message-ID: <6D6BD1DE8A5571489398B392A38A71579D25C32A@xmdb04.nch.kids> The Sulphation Toluidine Blue stain should work a treat and very quick to do: Sulphation - Toluidine Blue Technique for Fungi Following sulphation, fungi stain metachromatically with toluidine blue. Certain hydroxyl groups are esterified by sulphuric acid to form ester sulphate groups that stain metachromatically with toluidine blue (Smith & Lowrey 1986, Henwood et al 2013). Fixation: 10% buffered formalin Microtomy: 5?m paraffin sections Solutions: 1. Sulphation Reagent: For the sulphation reagent, 45 ml of glacial acetic acid is poured into a Coplin jar which has been placed into a plastic tub filled with cool tap water (not below 10?C). A 15-ml portion of concentrated sulfuric acid is slowly added with a glass pipette, being careful not to produce splashing. The solution is gently mixed with a glass rod. The Coplin jar is then sealed with petroleum jelly. The sulphation reagent is kept at room temperature and can be used for 1 week (Gosey et al 1985). 2. 3% Acetic Acid. 3. Toluidine Blue O Solution: Toluidine Blue O (CI 52040) 0.01g 3% Acetic Acid 100ml 4. Metanil Yellow Counterstain: Metanil yellow (CI 13065) 0.1g Distilled Water 100ml Glacial Acetic Acid 0.1ml 5. Absolute Acetone. Controls: Fungi containing tissue. Procedure: 1. Cut frozen sections, air dry, defat in methanol 1 minute, rinse in water 2. Dry briefly, cover with sulphation reagent, 10 minutes. 3. Wash in running water, 5 minutes 4. Place in 3% acetic acid, 1 minute. 5. Stain in Toluidine Blue O solution, 3 minutes. 6. Rinse in 3% acetic acid, 1 minute. 7. Differentiate in absolute acetone, 5 seconds. 8. Rinse in distilled water, 5 seconds. 9. Counterstain in Metanil Yellow solution, 6 seconds. 10. Rinse in distilled water, 5 seconds. 11. Blot, dry and mount. Results: Fungi stained purple to red against a yellow background. Smith DJ, Lowrey T, (1986) "A Modified Sulfation-Toluidine Blue Technique for the demonstration of fungi in tissue sections" J Histotechnol. 9(1):23-24. Gosey LL, Howard RM, Witebsky FG, Ognibene FP et al (1985) "Advantages of a Modified Toluidine Blue 0 Stain and Bronchoalveolar Lavage for the Diagnosis of Pneumocystis carinii Pneumonia" J Clin Microbiol 22(5):803-807. Henwood, A. F., Prasad, L., & Bourke, V. M. (2013). "The application of heated detergent dewaxing and rehydration to techniques for the demonstration of fungi: a comparison to routine xylene-alcohol dewaxing" J Histotechnol, early online DOI 10.1179/2046023613Y.0000000025. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of wrglo5@aol.com Sent: Friday, 17 May 2013 2:01 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] fungal stain for frozen sections Hi, I work in a Surgical Pathology frozen section lab outside the OR rooms. Normally we only perform an H&E on the frozens. Now we are looking for a fast and inexpensive stain for fungus on our frozen sections. Fast is the operative word because we have to report diagnoses back to ORs in a 20 minute timeperiod. Any ideas to help? Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From robinsoc <@t> mercyhealth.com Fri May 17 08:38:54 2013 From: robinsoc <@t> mercyhealth.com (Cynthia Robinson) Date: Fri May 17 08:39:02 2013 Subject: [Histonet] urine hemosiderin Message-ID: <5195EC9E020000AF0000E359@nodcdmg2.no.trinity-health.org> What kind of slide preparation are you using for urine? Cytospin vs thin prep vial is the discussion we are having. And are you doing a manual or automated iron stain? Thanks for any info you can provide. Cindi Robinson HT(ASCP) Mercy Medical Center Dunes Medical Laboratories 350 W Anchor Dr Dakota Dunes SD 57049 phone-712-279-2768 robinsoc@mercyhealth.com From GauchV <@t> mail.amc.edu Fri May 17 09:28:35 2013 From: GauchV <@t> mail.amc.edu (Gauch, Vicki) Date: Fri May 17 09:28:44 2013 Subject: [Histonet] Computers and Equipment Message-ID: Hi everyone, We are currently looking at LIS systems and equipment and are hoping to get some opinions. If anyone has any information on any of the following questions, I would really appreciate your help.... - For hospital based labs (~ 40,000-60,000 surgical cases/year)- what computer system (and version) do you use in your lab? - Does it have bar code "tracking" system as part of it or do you use a "tracking" system from another vendor (and which one) ? - What type of cassette and slide labelers/engravers do you use? -What are the pros and cons of whatever system(s) and equipment you use? -How long have you been using your current computer system? -Are your pathologists satisfied with your computer system? Thank you in advance for your assistance and have a great weekend !! Vicki Gauch AMCH Albany, NY ----------------------------------------- CONFIDENTIALITY NOTICE: This email and any attachments may contain confidential information that is protected by law and is for the sole use of the individuals or entities to which it is addressed. If you are not the intended recipient, please notify the sender by replying to this email and destroying all copies of the communication and attachments. Further use, disclosure, copying, distribution of, or reliance upon the contents of this email and attachments is strictly prohibited. To contact Albany Medical Center, or for a copy of our privacy practices, please visit us on the Internet at www.amc.edu. From Aliete_Langsdorf <@t> MEEI.HARVARD.EDU Fri May 17 10:00:03 2013 From: Aliete_Langsdorf <@t> MEEI.HARVARD.EDU (Langsdorf, Aliete) Date: Fri May 17 10:00:11 2013 Subject: [Histonet] Cleaning Dissection Tools Message-ID: <84B03145D9971342951CDF0E003F88741800F9@isvifmbxsvr02.meei.harvard.edu> Hello Fellow Histonetters, I have some very fine small dissection tools (small Vannas scissors, Dumont #5s, etc) which I use for microdissections (under a dissecting scope.) Afterwards they are often stained with blood or have tissue debris stuck to them. Is there a standard protocol or particular type of soap that you use? I want to get the blood/tissue off, but am afraid of marring/bending the fine tips with scrubbing. I usually soak for a little while in warm soapy water and then lightly clean with a Kim-wipe, but is there a better way? I have also heard some people say they never use soap. Some of my tools I use with PFA and others I use solely on tissue (no PFA) if that makes a difference in how you clean. Thanks for any suggestions! ~Ally Langsdorf Research Technologist Ocular Genomics Institute Massachusetts Eye & Ear Infirmary Lab: (617)-573-6485 From tpodawiltz <@t> lrgh.org Fri May 17 10:32:25 2013 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Fri May 17 10:32:31 2013 Subject: [Histonet] urine hemosiderin In-Reply-To: <5195EC9E020000AF0000E359@nodcdmg2.no.trinity-health.org> References: <5195EC9E020000AF0000E359@nodcdmg2.no.trinity-health.org> Message-ID: <38667E7FB77ECD4E91BFAEB8D98638632500841395@LRGHEXVS1.practice.lrgh.org> We use ThinPrep. Tom Podawiltz HT (ASCP) Histology Section Head/Laboratory Safety Officer. LRGHealthcare Laconia, NH 03246 603-524-3211 ext: 3220 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cynthia Robinson Sent: Friday, May 17, 2013 9:39 AM To: histonet Subject: [Histonet] urine hemosiderin What kind of slide preparation are you using for urine? Cytospin vs thin prep vial is the discussion we are having. And are you doing a manual or automated iron stain? Thanks for any info you can provide. Cindi Robinson HT(ASCP) Mercy Medical Center Dunes Medical Laboratories 350 W Anchor Dr Dakota Dunes SD 57049 phone-712-279-2768 robinsoc@mercyhealth.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From CDavis <@t> che-east.org Fri May 17 12:19:41 2013 From: CDavis <@t> che-east.org (Davis, Cassie) Date: Fri May 17 12:22:07 2013 Subject: [Histonet] urine hemosiderin In-Reply-To: <92deee6a-3089-47d3-8b53-65244cc7fc5d@CHESXH02.one.ads.che.org> References: <92deee6a-3089-47d3-8b53-65244cc7fc5d@CHESXH02.one.ads.che.org> Message-ID: <08861B9CF6C7774E874635A4818AE37B08CFD5C6@CHEXCMS01.one.ads.che.org> Hematology does a cytospin, we fix it in 95%Reagent alcohol for 10 minutes then do the iron stain on the NEXES automatic special stainer. No complaints. Cassandra Davis CDavis@che-east.org 302-575-8095 Saint Francis Hospital Saintfrancishealthcare.org Saint Francis Facebook Page ________________________________________ Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From mkent <@t> dermpathlab.com Fri May 17 12:45:39 2013 From: mkent <@t> dermpathlab.com (Michael Kent) Date: Fri May 17 12:45:54 2013 Subject: [Histonet] Special Stainers In-Reply-To: <38667E7FB77ECD4E91BFAEB8D98638632500841395@LRGHEXVS1.practice.lrgh.org> References: <5195EC9E020000AF0000E359@nodcdmg2.no.trinity-health.org> <38667E7FB77ECD4E91BFAEB8D98638632500841395@LRGHEXVS1.practice.lrgh.org> Message-ID: <27EB7FFB1A15F549B17F79B1A856C70BCDB30B@dlcs-sbs1.DPLCS.intra> Hello All, Can you share your experience or recommend an automated Special Stainer for clinical path? We are considering adding an additional. Thanks, Mike From patrick.lewis <@t> seattlechildrens.org Fri May 17 13:21:15 2013 From: patrick.lewis <@t> seattlechildrens.org (Lewis, Patrick) Date: Fri May 17 13:21:28 2013 Subject: [Histonet] Can someone recommend a good means of storage for Cryomold blocks? Message-ID: <3903BE18914F4440834F0E620415FFCA2313215F@PPWEXD01a.childrens.sea.kids> Hi everyone, can someone recommend a good storage method for Frozen tissue blocks made from cryomolds? We have about 200 blocks. Currently we have them stored freezer boxes in bags with some wet ice at -70. It might be better if we had plastic tray bins to put the bags in, but I have not been able to find them yet. Anyone have a catalog number for those gray tray bins? Thanks Patrick. CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From flnails <@t> texaschildrens.org Fri May 17 13:25:59 2013 From: flnails <@t> texaschildrens.org (Nails, Felton) Date: Fri May 17 13:26:04 2013 Subject: [Histonet] RE: Special Stainers In-Reply-To: <27EB7FFB1A15F549B17F79B1A856C70BCDB30B@dlcs-sbs1.DPLCS.intra> References: <5195EC9E020000AF0000E359@nodcdmg2.no.trinity-health.org> <38667E7FB77ECD4E91BFAEB8D98638632500841395@LRGHEXVS1.practice.lrgh.org> <27EB7FFB1A15F549B17F79B1A856C70BCDB30B@dlcs-sbs1.DPLCS.intra> Message-ID: <327E034F1892504289B7A17EC71DF9F3E7A6@TCFMSG03.ad.texaschildrenshospital.org> I would like to be included on this request also, We currently have the DAKO special stainer and are looking to see what else is available. The DAKO unit is very good but we are being forced to sign a reagent agreement with a minimum commitment even though we own the unit. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael Kent Sent: Friday, May 17, 2013 12:46 PM To: histonet Subject: [Histonet] Special Stainers Hello All, Can you share your experience or recommend an automated Special Stainer for clinical path? We are considering adding an additional. Thanks, Mike _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ______________________________________________________________________ From tp2 <@t> medicine.wisc.edu Fri May 17 13:36:06 2013 From: tp2 <@t> medicine.wisc.edu (Thomas Pier) Date: Fri May 17 13:36:30 2013 Subject: [Histonet] Bioss conjugated primaries Message-ID: <51963247020000DF00016C78@gwmail.medicine.wisc.edu> Hello, Does anybody have any experience with conjugated primary antibodies from Bioss, particularly S100 and acetyl-histone H4? I have tried retrieval with citrate, blocking and primary incubations of an hour at room temp and overnight at 4 degrees withing the recomended dilution range. Thanks, Tom From Timothy.Morken <@t> ucsfmedctr.org Fri May 17 14:07:41 2013 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Fri May 17 14:07:49 2013 Subject: [Histonet] Can someone recommend a good means of storage for Cryomold blocks? In-Reply-To: <3903BE18914F4440834F0E620415FFCA2313215F@PPWEXD01a.childrens.sea.kids> References: <3903BE18914F4440834F0E620415FFCA2313215F@PPWEXD01a.childrens.sea.kids> Message-ID: <761E2B5697F795489C8710BCC72141FF080E15@ex07.net.ucsf.edu> Patrick, We use a metal rack for Fisher freezer boxes. The racks are from USA Scientific and are unique in that they slide from the front, so access is very easy. Cat# 2632-4550, for a 4 x 5 array, fitting 5" x 5" x 2" freezer boxes. http://www.usascientific.com/search.aspx?find=2632-4500 The freezer boxes are Thermo Fisher Scientific, Cat# 03-395-455 without dividers. Depending on the size of the tissue, we either store in 2 ml cryovials (kidney bx, 64 vials per box) or in 20ml vials (muscle, 9 vials per box). If dehydration is an issue, wrap in plastic wrap and aluminum foil and add a bit of frost to the vial. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lewis, Patrick Sent: Friday, May 17, 2013 11:21 AM To: 'Histonet@lists.utsouthwestern.edu' Subject: [Histonet] Can someone recommend a good means of storage for Cryomold blocks? Hi everyone, can someone recommend a good storage method for Frozen tissue blocks made from cryomolds? We have about 200 blocks. Currently we have them stored freezer boxes in bags with some wet ice at -70. It might be better if we had plastic tray bins to put the bags in, but I have not been able to find them yet. Anyone have a catalog number for those gray tray bins? Thanks Patrick. CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kiran_g <@t> sbcglobal.net Fri May 17 14:58:52 2013 From: kiran_g <@t> sbcglobal.net (Kiranjit Grewal) Date: Fri May 17 14:59:03 2013 Subject: [Histonet] RE: Special Stainers In-Reply-To: <327E034F1892504289B7A17EC71DF9F3E7A6@TCFMSG03.ad.texaschildrenshospital.org> References: <5195EC9E020000AF0000E359@nodcdmg2.no.trinity-health.org> <38667E7FB77ECD4E91BFAEB8D98638632500841395@LRGHEXVS1.practice.lrgh.org> <27EB7FFB1A15F549B17F79B1A856C70BCDB30B@dlcs-sbs1.DPLCS.intra> <327E034F1892504289B7A17EC71DF9F3E7A6@TCFMSG03.ad.texaschildrenshospital.org> Message-ID: <00E31D72-F964-45C2-9918-EBBF49490E7A@sbcglobal.net> Vantana has good system, we have 11 units. Kiran Sent from my iPhone On May 17, 2013, at 11:25 AM, "Nails, Felton" wrote: > I would like to be included on this request also, We currently have the DAKO special stainer and are looking to see what else is available. > The DAKO unit is very good but we are being forced to sign a reagent agreement with a minimum commitment even though we own the unit. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael Kent > Sent: Friday, May 17, 2013 12:46 PM > To: histonet > Subject: [Histonet] Special Stainers > > Hello All, > > Can you share your experience or recommend an automated Special Stainer for clinical path? We are considering adding an additional. > > Thanks, > > Mike > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ______________________________________________________________________ > CONFIDENTIALITY NOTICE: > The information in this e-mail may be confidential and/or > privileged. If you are not the intended recipient or an > authorized representative of the intended recipient, you > are hereby notified that any review, dissemination, or > copying of this e-mail and its attachments, if any, or > the information contained herein is prohibited. If you > have received this e-mail in error, please immediately > notify the sender by return e-mail and delete this e-mail > from your computer system. Thank you. > ______________________________________________________________________ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Lynn.Burton <@t> Illinois.gov Fri May 17 15:40:15 2013 From: Lynn.Burton <@t> Illinois.gov (Burton, Lynn) Date: Fri May 17 15:40:23 2013 Subject: [Histonet] RE: Special Stainers In-Reply-To: <00E31D72-F964-45C2-9918-EBBF49490E7A@sbcglobal.net> References: <5195EC9E020000AF0000E359@nodcdmg2.no.trinity-health.org> <38667E7FB77ECD4E91BFAEB8D98638632500841395@LRGHEXVS1.practice.lrgh.org> <27EB7FFB1A15F549B17F79B1A856C70BCDB30B@dlcs-sbs1.DPLCS.intra> <327E034F1892504289B7A17EC71DF9F3E7A6@TCFMSG03.ad.texaschildrenshospital.org> <00E31D72-F964-45C2-9918-EBBF49490E7A@sbcglobal.net> Message-ID: I have been very pleased with the Discovery and Benchmark from Ventana. Lynn Burton -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kiranjit Grewal Sent: Friday, May 17, 2013 2:59 PM To: Nails, Felton Cc: histonet Subject: Re: [Histonet] RE: Special Stainers Vantana has good system, we have 11 units. Kiran Sent from my iPhone On May 17, 2013, at 11:25 AM, "Nails, Felton" wrote: > I would like to be included on this request also, We currently have the DAKO special stainer and are looking to see what else is available. > The DAKO unit is very good but we are being forced to sign a reagent agreement with a minimum commitment even though we own the unit. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Michael Kent > Sent: Friday, May 17, 2013 12:46 PM > To: histonet > Subject: [Histonet] Special Stainers > > Hello All, > > Can you share your experience or recommend an automated Special Stainer for clinical path? We are considering adding an additional. > > Thanks, > > Mike > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ______________________________________________________________________ > CONFIDENTIALITY NOTICE: > The information in this e-mail may be confidential and/or privileged. > If you are not the intended recipient or an authorized representative > of the intended recipient, you are hereby notified that any review, > dissemination, or copying of this e-mail and its attachments, if any, > or the information contained herein is prohibited. If you have > received this e-mail in error, please immediately notify the sender by > return e-mail and delete this e-mail from your computer system. Thank > you. > ______________________________________________________________________ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mucram11 <@t> comcast.net Fri May 17 17:55:59 2013 From: mucram11 <@t> comcast.net (Pam Marcum) Date: Fri May 17 17:56:21 2013 Subject: [Histonet] RE: Special Stainers In-Reply-To: Message-ID: <844560921.16163.1368831359048.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> We have two Ar tisan st ainers and we have been extremely happy with them.? The service and price were both unbeatable.? We did have two special stainers that we did not like and our Pathologists were very unhappy with the throughput and stains.? Pamela Marcum UAMS ----- Original Message ----- From: "Lynn Burton" To: "Kiranjit Grewal" , "Felton Nails" Cc: "histonet" Sent: Friday, May 17, 2013 3:40:15 PM Subject: RE: [Histonet] RE: Special Stainers I have been very pleased with the Discovery and Benchmark from Ventana. Lynn Burton -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kiranjit Grewal Sent: Friday, May 17, 2013 2:59 PM To: Nails, Felton Cc: histonet Subject: Re: [Histonet] RE: Special Stainers Vantana has good system, we have 11 units. Kiran Sent from my iPhone On May 17, 2013, at 11:25 AM, "Nails, Felton" wrote: > I would like to be included on this request also, We currently have the DAKO special stainer and are looking to see what else is available. > The DAKO unit is very good but we are being forced to sign a reagent agreement with a minimum commitment even though we own the unit. ? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Michael Kent > Sent: Friday, May 17, 2013 12:46 PM > To: histonet > Subject: [Histonet] Special Stainers > > Hello All, > > Can you share your experience or recommend an automated Special Stainer for clinical path? We are considering adding an additional. > > Thanks, > > Mike > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ______________________________________________________________________ > CONFIDENTIALITY NOTICE: > The information in this e-mail may be confidential and/or privileged. ? > If you are not the intended recipient or an authorized representative > of the intended recipient, you are hereby notified that any review, > dissemination, or copying of this e-mail and its attachments, if any, > or the information contained herein is prohibited. ?If you have > received this e-mail in error, please immediately notify the sender by > return e-mail and delete this e-mail from your computer system. ?Thank > you. > ______________________________________________________________________ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From latecor <@t> montevideo.com.uy Fri May 17 21:09:58 2013 From: latecor <@t> montevideo.com.uy (C.D.G.) Date: Fri May 17 21:10:19 2013 Subject: [Histonet] Slee microtome References: <201305172250130587.0018E6B1@adinet.com.uy> Message-ID: <201305172309580446.0004C705@smtp.montevideo.com.uy> I would like to receive any feedback about Slee rotary microtomes, I will buy one unit but don't have any experience with this brand. Please, I will be gratefull if you can send any comments of its performance. My thanks in advance, Carlos.- From latecor <@t> montevideo.com.uy Sat May 18 09:51:56 2013 From: latecor <@t> montevideo.com.uy (C.D.G.) Date: Sat May 18 09:52:09 2013 Subject: [Histonet] please I need some imput about Slee microtomes Message-ID: <201305181151560203.0009811B@smtp.montevideo.com.uy> As I told in earlier mail, we are about to buy a rotary microtome from Slee. Leica distributor is not giving us any quotation, so it seems that he is not interested on selling microtomes. So we need you, the Histonet knowledge, to give us any good or bad experiences you had with this brand. My kind regards for you all, Carlos Defeo histotechnologist From rjbuesa <@t> yahoo.com Sat May 18 10:22:24 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat May 18 10:22:27 2013 Subject: [Histonet] please I need some imput about Slee microtomes In-Reply-To: <201305181151560203.0009811B@smtp.montevideo.com.uy> References: <201305181151560203.0009811B@smtp.montevideo.com.uy> Message-ID: <1368890544.44712.YahooMailNeo@web163101.mail.bf1.yahoo.com> My question to you is: why are you going to buy a microtome you know nothing about? The microtome is one essential tool in your lab and the quality of the sections depend on it. Are you going to purchase that "Slee" because of budget constrains? You have to realize that you will get?what you pay for, or even it can be expensive and?unreliable. I for one have never heard of that brand. If the Leica representative does not want to quote you a microtome, visit?Leica Microsystems web site and complain about that. There are several brands of microtomes of great quality, Leica being one of them (for me it is the best), but Microm and the ones sold by Sakura are also good. Do not rush to buy a microtome that at the end will cause you problems. Save in other purchases, but not on micrtome. That is my advise Ren? J.? From: C.D.G. To: Histonet@lists.utsouthwestern.edu Cc: Histonet@lists.utsouthwestern.edu Sent: Saturday, May 18, 2013 10:51 AM Subject: [Histonet] please I need some imput about Slee microtomes As I told in earlier mail, we are about to buy a rotary microtome from Slee. Leica distributor is not giving us any quotation, so it seems that he is not interested on selling microtomes. So we need you,? the Histonet knowledge, to give us any good or bad experiences you had with this brand. My kind regards for you all, Carlos Defeo histotechnologist _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> gmail.com Sat May 18 14:38:31 2013 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Sat May 18 14:38:47 2013 Subject: [Histonet] Re: urine hemosiderin Message-ID: Urine hemosiderin is an archaic procedure that still may be in the handy-dandy intern's guide, along with serum haptoglobin, as part of working up a suspected transfusion reaction. An order for either of these tests needs to be promptly reviewed to see if a doctor is trying to do an amateur transfusion reaction workup. That's a bit of an emergency. About thirty years ago I wrote this procedure, on two different occasions. I've appended both of them. Bob Richmond Samurai Pathologist Maryville TN ************************************ URINE HEMOSIDERIN PRINCIPLE: The iron-containing pigment hemosiderin is found in the urine, either free or within casts and epithelial cells, in diseases in which this pigment is lost through the kidneys. Hemosiderin may be seen on direct examination of urine sediment, and its presence confirmed with the Perls reaction for stainable iron. SPECIMEN: A random urine specimen with the sediment in good condition for examination is required. Do not perform the test unless a suitable specimen is available. Urine hemosiderin is not a stat procedure, and is not part of the routine work-up of a suspected transfusion reaction. REAGENTS: 2% solution of potassium ferrocyanide in distilled water 1% solution of concentrated hydrochloric acid in distilled water EQUIPMENT: The same equipment is required as for routine examination of urinary sediment. Be sure that all materials are clean, since the iron stain reacts with many contaminant materials in the environment. PROCEDURE: 1. Prepare centrifuged urinary sediment in the usual fashion. 2. Examine the sediment for hemosiderin granules. These are yellow-brown granules which may be free in the sediment, or may be inside of epithelial cells or casts. The granules are brilliantly refractile; that is, they ?light up? when you rack the condenser of the microscope down. 3. Add to the rest of the sediment in the tube: 5 mL of 2% potassium ferrocyanide 5 mL of 1% hydrochloric acid 4. let stand 10 minutes 5. centrifuge 6. examine under the microscope RESULTS: Hemosiderin appears as bright blue granules of ferric ferrocyanide after staining. No such material is present in normal urine. Unfortunately no satisfactory positive control is available. Review the material with the pathologist if you are uncertain of your results. Report as urine hemosiderin: negative OR positive. REFERENCES: 1. Rous, P. Urinary siderosis. J Exper Med 1918; 28:645. This very old article contains the original method, cited in Todd-Sanford and other modern works. 2. Bradley M, Schumann GB, Ward CJW: Examination of urine, Todd-Sanford-Davidsohn Clinical Diagnosis and Management by Laboratory Methods. 16th edition. Edited by JB Henry. Philadelphia, Saunders Company, 1979, p 630. ****************************************** URINE HEMOSIDERIN PRINCIPLE: Hemosiderin may appear in renal tubule cells as a result of excessive iron storage, or two to three days after an acute hemolytic episode. Hemosiderin granules may as a result appear in the urine. This test is usually ordered in suspected hemolytic transfusion reaction, though it is not of much diagnostic value in this situation. PATIENT PREPARATION: none SPECIMEN: Any fresh urine specimen may be tested. REAGENTS: 2% potassium ferrocyanide (wt/vol), prepared fresh, measurements approximate 1% (vol/vol) hydrochloric acid DIRECTIONS: 1. Prepare sediment from at least 10 mL of urine. 2. Examine the sediment under the microscope in the usual fashion. Look for coarse golden-brown highly refractile granules, preferably within epithelial cells or casts. You may need the help of the pathologist to do this. If no hemosiderin granules are found, do not proceed with the test, and report ?no urine hemosiderin identified?. 3. If hemosiderin granules are seen, confirm their identity by suspending the sediment in a fresh mixture of equal parts of potassium ferrocyanide and hydrochloric acid. Allow it to stand for 10 to 30 minutes. 4. Centrifuge, and examine the sediment again. The hemosiderin granules should now appear dark blue. Foreign matter often stains blue, and can cause false positive interpretations. Once again, you may need the help of the pathologist. 5. If hemosiderin granules are definitely identified, report ?urine hemosiderin present?. CONTROLS: Run a clean normal urine as a negative control. There is no satisfactory positive control available. A patient with hemochromatosis (they?re often subjected to repeated therapeutic phlebotomy) may have hemosiderinuria. REFERENCES: 1. Rous, P. Urinary siderosis. J. Exper Med 28: 645, 1918. This ancient article is cited in successive editions of Todd-Sanford (now John B. Henry). AUTHOR: Robert S. Richmond, M.D., F.C.A.P. From latecor <@t> montevideo.com.uy Sat May 18 16:21:51 2013 From: latecor <@t> montevideo.com.uy (C.D.G.) Date: Sat May 18 16:22:03 2013 Subject: [Histonet] Slee brand microtome Message-ID: <201305181821510687.0014C1E7@smtp.montevideo.com.uy> Thank you all for your responses, I know that Leica is the best in microtomy. The fact I am stating is that the only local distributor ( i am in Uruguay,South America), is not giving us any attention. We asked him many times for a quotation, but nothing happened. We have for now the quotation from Slee distributor, and so we asked you, people at the Histonet, any opinions about this brand. We are not in the way of saving money, this is only an availability question. Thank you all again and be kind to give any more suggestions if you can. My regards, Carlos.- From arvidsonkristen <@t> yahoo.com Sun May 19 10:22:34 2013 From: arvidsonkristen <@t> yahoo.com (kristen arvidson) Date: Sun May 19 10:22:44 2013 Subject: [Histonet] OCT Code Cuts Message-ID: <1368976954.72483.YahooMailAndroidMobile@web122206.mail.ne1.yahoo.com> What kind of impacts is everyone seeing post cpt cuts?? I am concerned for the future of our profession. Sent from Yahoo! Mail on Android From brannon <@t> alliedsearchpartners.com Mon May 20 10:54:56 2013 From: brannon <@t> alliedsearchpartners.com (Brannon Owens) Date: Mon May 20 10:55:07 2013 Subject: [Histonet] Accessioning Supervisor- southwest FL Message-ID: ASP the leader in permanent placement of laboratory professionals has a job opening for an Accessioning Supervisor in Florida. For a full job description email Brannon Owens at brannon@alliedsearchparnters.com. To view a complete list of Allied Search Partners current openings go to: http://www.alliedsearchpartners.com/careers.php -- Brannon Owens Recruitment Manager Allied Search Partners From Jonathan.Cremer <@t> med.kuleuven.be Tue May 21 02:01:52 2013 From: Jonathan.Cremer <@t> med.kuleuven.be (Jonathan Cremer) Date: Tue May 21 02:02:03 2013 Subject: [Histonet] RE: Cleaning Dissection Tools In-Reply-To: <84B03145D9971342951CDF0E003F88741800F9@isvifmbxsvr02.meei.harvard.edu> References: <84B03145D9971342951CDF0E003F88741800F9@isvifmbxsvr02.meei.harvard.edu> Message-ID: I rinse off my microdissection tools right after use, so any gross contamination is gone before it has a chance to dry and stick like superglue. I then soak them in demi water with some handsoap for 15-30 minutes, and scrub with an old toothbrush. To avoid damage to the cutting edges, lay the blade flat against your finger and brush from the spine to the cutting edge. Repeat on the other side. Never brush into the cutting edge! You don't need pressure for scrubbing, the soak will 'soften' all the stains. Finally, rinse under running water, shake off excess and blot dry with Kimwipes. You can use compressed air to blow water out of the hinges and small places you can't get at. For good measure, I store my tools in a box with some silica packs. Best, Jonathan --- ________________________________________ Van: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] namens Langsdorf, Aliete [Aliete_Langsdorf@MEEI.HARVARD.EDU] Verzonden: vrijdag 17 mei 2013 17:00 To: histonet@lists.utsouthwestern.edu Onderwerp: [Histonet] Cleaning Dissection Tools Hello Fellow Histonetters, I have some very fine small dissection tools (small Vannas scissors, Dumont #5s, etc) which I use for microdissections (under a dissecting scope.) Afterwards they are often stained with blood or have tissue debris stuck to them. Is there a standard protocol or particular type of soap that you use? I want to get the blood/tissue off, but am afraid of marring/bending the fine tips with scrubbing. I usually soak for a little while in warm soapy water and then lightly clean with a Kim-wipe, but is there a better way? I have also heard some people say they never use soap. Some of my tools I use with PFA and others I use solely on tissue (no PFA) if that makes a difference in how you clean. Thanks for any suggestions! ~Ally Langsdorf Research Technologist Ocular Genomics Institute Massachusetts Eye & Ear Infirmary Lab: (617)-573-6485 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cmconway <@t> usgs.gov Tue May 21 10:25:50 2013 From: cmconway <@t> usgs.gov (Conway, Carla) Date: Tue May 21 10:26:21 2013 Subject: [Histonet] Xylene substitutes for tissue processor clean cycle Message-ID: Hello all, The technician who does preventive maintenance on our LX-120 tissue processor recommended that we use xylene as the solvent in our clean cycle. We are considering using a xylene substitue in the clean cycle and would appreciate any recommendations. Thanks very much, Carla Conway Histology Technician Western Fisheries Research Center, USGS 6505 N.E. 65th Street Seattle, WA 98115-5016 USA Phone: 206-526-6282 ext. 242 Fax: 206-526-6654 E-mail: cmconway@usgs.gov From litepath2000 <@t> yahoo.com Tue May 21 10:27:13 2013 From: litepath2000 <@t> yahoo.com (NYSHisto) Date: Tue May 21 10:27:18 2013 Subject: [Histonet] Job Oppurtunity: Suffolk County ME Office Message-ID: <1369150033.34491.YahooMailNeo@web141202.mail.bf1.yahoo.com> Posting on behalf of the Suffolk County Chief ME: The Suffolk County Office of the Medical Examiner, located in Hauppauge, New York, is seeking to hire a Histology Technician. Qualified individuals will prepare stains, mount slides, and perform general histology duties. The candidate will work under the direct supervision of the Laboratory Technician, and prepare slides at the request of the Medical Forensic Pathologists i.e., specimen collection, specimen processing, tissue embedding, microtomy, routine staining and data entry. MINIMUM REQUIREMENTS: CANDIDATES WILL ONLY BE CONSIDERED IF ONE OF THE FOLLOWING REQUIREMENTS ARE MET: a) Graduation from a New York State or Regionally accredited college or university with a Bachelor's degree in Science including or supplemented by coursework in the preparation and sectioning of tissues for staining and tissue identification; or, b) Completion of a two (2) year course of instruction in histological laboratory techniques; or c) Two (2) years of experience in a laboratory using histological techniques; or d) A satisfactory equivalent combination of the above education and experience. Competitive Salary and Benefit Package Suffolk County is an Equal Opportunity Employer For more Information please contact Leslie Smith at Leslie.Smith2@suffolkcountyny.gov ? ------- Luis Chiriboga Ph.D. President, New York State Histotechnological Society NYSHS Website: www.nyhisto.org NYSHS Message Board: http://tech.groups.yahoo.com/group/NYSHS1972/ This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email. From rjbuesa <@t> yahoo.com Tue May 21 10:38:40 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue May 21 10:38:45 2013 Subject: [Histonet] Xylene substitutes for tissue processor clean cycle In-Reply-To: References: Message-ID: <1369150720.74014.YahooMailNeo@web163103.mail.bf1.yahoo.com> Use a 5% solution of a strong lab detergent in isopropyl alcohol. Since the cleaning cycle is completed at 50?C that will be enough and you will not have to expose yourself to xylene vapors. Ren? J. From: "Conway, Carla" To: histonet@lists.utsouthwestern.edu Sent: Tuesday, May 21, 2013 11:25 AM Subject: [Histonet] Xylene substitutes for tissue processor clean cycle Hello all, The technician who does preventive maintenance on our LX-120 tissue processor recommended that we use xylene as the solvent in our clean cycle. We are considering using a xylene substitue in the clean cycle and would appreciate any recommendations. Thanks very much, Carla Conway Histology Technician Western Fisheries Research Center, USGS 6505 N.E. 65th Street Seattle, WA 98115-5016 USA Phone: 206-526-6282 ext. 242 Fax: 206-526-6654 E-mail: cmconway@usgs.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Tue May 21 10:41:18 2013 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Tue May 21 10:41:24 2013 Subject: [Histonet] Xylene substitutes for tissue processor clean cycle In-Reply-To: References: Message-ID: <14E2C6176416974295479C64A11CB9AE016411AC2DC0@SBS2K8.premierlab.local> Carla I think most tissue processor vendors recommend xylene for cleaning and do not recommend a xylene substitute. This may have changed recently but I would suspect it would void your warranty if you were still under one if the vendor recommends xylene and you are using a xylene substitute for cleaning. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Laboratory Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 Work (303) 682-3949 Fax (303) 682-9060 Cell (303) 881-0763 liz@premierlab.com www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Conway, Carla Sent: Tuesday, May 21, 2013 9:26 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Xylene substitutes for tissue processor clean cycle Hello all, The technician who does preventive maintenance on our LX-120 tissue processor recommended that we use xylene as the solvent in our clean cycle. We are considering using a xylene substitue in the clean cycle and would appreciate any recommendations. Thanks very much, Carla Conway Histology Technician Western Fisheries Research Center, USGS 6505 N.E. 65th Street Seattle, WA 98115-5016 USA Phone: 206-526-6282 ext. 242 Fax: 206-526-6654 E-mail: cmconway@usgs.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek <@t> digestivespecialists.com Tue May 21 10:41:08 2013 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Tue May 21 10:43:59 2013 Subject: [Histonet] Xylene substitutes for tissue processor clean cycle In-Reply-To: References: Message-ID: <5A2BD13465E061429D6455C8D6B40E391654826895@IBMB7Exchange.digestivespecialists.com> Carla, We use Formula 83 in the clean cycle. We do change it more often than you would xylene though. Linda -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Conway, Carla Sent: Tuesday, May 21, 2013 11:26 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Xylene substitutes for tissue processor clean cycle Hello all, The technician who does preventive maintenance on our LX-120 tissue processor recommended that we use xylene as the solvent in our clean cycle. We are considering using a xylene substitue in the clean cycle and would appreciate any recommendations. Thanks very much, Carla Conway Histology Technician Western Fisheries Research Center, USGS 6505 N.E. 65th Street Seattle, WA 98115-5016 USA Phone: 206-526-6282 ext. 242 Fax: 206-526-6654 E-mail: cmconway@usgs.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TMcNemar <@t> lmhealth.org Tue May 21 11:01:17 2013 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Tue May 21 11:01:29 2013 Subject: [Histonet] Xylene substitutes for tissue processor clean cycle In-Reply-To: References: Message-ID: We use Americlear. Have not used Xylene for many, many years. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Conway, Carla Sent: Tuesday, May 21, 2013 11:26 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Xylene substitutes for tissue processor clean cycle Hello all, The technician who does preventive maintenance on our LX-120 tissue processor recommended that we use xylene as the solvent in our clean cycle. We are considering using a xylene substitue in the clean cycle and would appreciate any recommendations. Thanks very much, Carla Conway Histology Technician Western Fisheries Research Center, USGS 6505 N.E. 65th Street Seattle, WA 98115-5016 USA Phone: 206-526-6282 ext. 242 Fax: 206-526-6654 E-mail: cmconway@usgs.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From mwich <@t> 7thwavelabs.com Tue May 21 12:04:01 2013 From: mwich <@t> 7thwavelabs.com (Michele Wich) Date: Tue May 21 12:04:08 2013 Subject: [Histonet] replacement for Dako's anti-mouse Ki67 Message-ID: Can anyone recommend a good Ki67 antibody for mouse tissue that is not raised in rabbit? I am looking for an antibody to replace Dako's monoclonal rat anti-mouse (TEC-3) Ki67 which has been discontinued. I appreciate any suggestions, Michele From jdbradley <@t> cmh.edu Tue May 21 12:05:05 2013 From: jdbradley <@t> cmh.edu (Bradley, Joshua, D) Date: Tue May 21 12:05:11 2013 Subject: [Histonet] Frozen Tissue Retention Message-ID: I am curious as to the frozen tissue retention policies in other institutions, especially in children's facilities. How long are samples considered diagnostically viable and relevant to patient care? What is done with the tissue when the time limit is reached? Our facility is interested in using old samples in research applications (unidentified of course). We currently have a retention policy of 10 years, but wanted to gauge how other facilities approach this matter. Thanks for any help that can be provided. Joshua D. Bradley, B.S., HT(ASCP)CM Clinical Lab Supervisor-Histology Children's Mercy Hospital Kansas City, MO ________________________________ Electronic mail from Children's Mercy Hospitals and Clinics. This communication is intended only for the use of the addressee. It may contain information that is privileged or confidential under applicable law. If you are not the intended recipient or the agent of the recipient, you are hereby notified that any dissemination, copy or disclosure of this communication is strictly prohibited. If you have received this communication in error, please immediately forward the message to Children's Mercy Hospital's Information Security Officer via return electronic mail at informationsecurityofficer@cmh.edu and expunge this communication without making any copies. Thank you for your cooperation. From AGleiberman <@t> cbiolabs.com Tue May 21 12:29:09 2013 From: AGleiberman <@t> cbiolabs.com (Anatoli Gleiberman) Date: Tue May 21 12:29:22 2013 Subject: [Histonet] RE: replacement for Dako's anti-mouse Ki67 In-Reply-To: References: Message-ID: <77BC2EEB6AC66C49AEF794DC98BE314C018D57809E@cbiolabs05.CBiolabs.local> Michele, BD Pharmingen, cat.# 556003, mouse monoclonal. I have used extensively the same clone B56 labeled with AlexaFluor488 and 555 - all they work very well on mouse and human tissues. Anatoli Gleiberman, PhD Director of Histopathology Cleveland Biolabs, Inc 73 High Street Buffalo, NY 14203 phone:716-849-6810 ext.354 fax:716-849-6817 e-mail: AGleiberman@cbiolabs.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michele Wich Sent: Tuesday, May 21, 2013 1:04 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] replacement for Dako's anti-mouse Ki67 Can anyone recommend a good Ki67 antibody for mouse tissue that is not raised in rabbit? I am looking for an antibody to replace Dako's monoclonal rat anti-mouse (TEC-3) Ki67 which has been discontinued. I appreciate any suggestions, Michele _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information. From Jessica <@t> nsh.org Tue May 21 12:46:27 2013 From: Jessica <@t> nsh.org (Jessica Smith) Date: Tue May 21 12:46:37 2013 Subject: [Histonet] NSH Summer Symposium Message-ID: <4FC64E7CF6FEC4428E6C6BFC49FAFBA66AE878@NSH-SRVR01.nsh.local> Hi Everyone! The National Society for Histotechnology is back in Las Vegas, NV for its 6th Annual Summer Symposium June 17-18, 2013! General sessions and workshops featuring expert speakers will provide you with the tools, advice and guidance you seek in your professional career. The Summer Symposium is one of the best values for your training dollars in histology education offering, 12 continuing education credits and an Exhibit Fair for one low price! HT Eligible? Don't miss out on the full day HT Readiness Course on Monday, June 17. Below are some links to help you plan your trip to the Sin City! Registration Information - Including fees, exhibit information, etc. Agenda - Complete schedule including speakers and abstracts Travel - Hotel & Flight information - **Important Note: Book your room by Wednesday, May 22 to guarantee the NSH Group rate! Register Online - Register today online PDF Brochure - Includes a registration form, agenda, and travel information Unable to view the links above? You can find all of the information using this URL: https://s3.goeshow.com/nsh/SS2013/ereg711677.cfm?pg=home Hope to see you in Vegas! Jessica Pellegrini Meeting Coordinator/Social Media Specialist National Society for Histotechnology 8850 Stanford Blvd. Suite 2900 Columbia, MD 21045 Phone: 443-535-4062 Fax:443-535-4055 Jessica@nsh.org | www.nsh.org www.histoconvention.org Follow us online for the latest news/updates! Facebook Twitter Linked In YouTube From Pat.Bell <@t> ucdenver.edu Tue May 21 14:25:58 2013 From: Pat.Bell <@t> ucdenver.edu (Bell, Pat) Date: Tue May 21 14:26:21 2013 Subject: [Histonet] CK5 and p63 Message-ID: <64DB27005E2FD3439E88502D7A5C9121010206489F70@CORTEZ.ucdenver.pvt> Hello to Everyone, Has anyone had experience doing CK5 and p63 in the rat? Could you please tell me where you get your antibody? I currently use Biocare's for human and will try it this week on the rat. I just was wondering if anyone had any other ideas. Thank you for your help. Pat Pat Bell HT(ASCP) University of Colorado, Denver Medical Oncology 12801 E 17th Ave, MS8117 Aurora, Colorado 80045 303-724-6077 pat.bell@ucdenver.edu From madelinegi <@t> yahoo.com Tue May 21 15:20:07 2013 From: madelinegi <@t> yahoo.com (Madeline Gi) Date: Tue May 21 15:20:14 2013 Subject: [Histonet] CAP question Message-ID: <1369167607.37966.YahooMailClassic@web163905.mail.gq1.yahoo.com> I have a question which was asked before but I don't remember the answer, how many CEU's are required?by CAP for a histologist in New York City. Thanks in?advance? Madeline Rotger Milanese H.T. BSHCS 500 New Hempstead Rd. New City N.Y. 10965 845-362-3200 Ext 129 madelinegi@yahoo.com From lpwenk <@t> sbcglobal.net Tue May 21 17:07:28 2013 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Tue May 21 17:07:34 2013 Subject: [Histonet] CAP question In-Reply-To: <1369167607.37966.YahooMailClassic@web163905.mail.gq1.yahoo.com> References: <1369167607.37966.YahooMailClassic@web163905.mail.gq1.yahoo.com> Message-ID: <718BB7CBD83B4AA5B7A3C289D9D40157@HP2010> CAP does not put any number as to how much CE is required for working techs. Just that there is a CE program (54200), and that there is a record of CE in the personnel records (54400, #6). GEN.54200 Continuing Education Phase I There is a functional continuing clinical laboratory education program adequate to meet the needs of all personnel. Evidence of Compliance: Written policy for continuing laboratory education GEN.54400 Personnel Records Phase II Personnel files are maintained on all current technical personnel and personnel records include all of the following items. 1. Summary of training and experience 2. Copy of academic degree or transcript 3. License, if required by state 4. Certification, if required by state or employer 5. Description of current duties and responsibilities as specified by the laboratory director: a) Procedures the individual is authorized to perform, b) Whether supervision is required for specimen processing, test performance or result reporting, c) Whether supervisory or director review is required to report patient test results 6. Records of continuing education 7. Records of radiation exposure where applicable (such as with in vivo radiation testing), but not required for low exposure levels such as certain in-vitro testing 8. Work-related incident and/or accident records 9. Dates of employment However, the CMP (Competency Maintenance programP of ASCP says, if you are a certified tech (certified on or after Jan. 1, 2004), you need 36 hours CE every 3 years, to maintain your certification. http://ascp.org/PDF/BOC-PDFs/CMP/CMPBooklet.aspx Peggy A. Wenk, HTL(ASCP)SLS Beaumont Health System Royal Oak, MI 48073 The opinions expressed are mine, and do not reflect my place of employment. -----Original Message----- From: Madeline Gi Sent: Tuesday, May 21, 2013 4:20 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP question I have a question which was asked before but I don't remember the answer, how many CEU's are required by CAP for a histologist in New York City. Thanks in advance Madeline Rotger Milanese H.T. BSHCS 500 New Hempstead Rd. New City N.Y. 10965 845-362-3200 Ext 129 madelinegi@yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vavalos <@t> allergydermatology.com Tue May 21 19:01:21 2013 From: vavalos <@t> allergydermatology.com (Vanessa Avalos) Date: Tue May 21 19:01:43 2013 Subject: [Histonet] Xylene substitutes for tissue processor clean cycle In-Reply-To: References: Message-ID: <4e37847c2f0e45eb95a7f414140be263@BLUPR07MB020.namprd07.prod.outlook.com> I use a excelsior and was told to always use Xylene in the cleaning cycle. Since sub is not as pure as xylene ( obviously) you could be at risk of developing paraffin clogs that the sub may not get. Using one xylene in the cleaning has not effected the odor at all in the lab. Good Luck. V.Avalos ADS, INC Fax:602-277-2134 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Conway, Carla Sent: Tuesday, May 21, 2013 8:26 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Xylene substitutes for tissue processor clean cycle Hello all, The technician who does preventive maintenance on our LX-120 tissue processor recommended that we use xylene as the solvent in our clean cycle. We are considering using a xylene substitue in the clean cycle and would appreciate any recommendations. Thanks very much, Carla Conway Histology Technician Western Fisheries Research Center, USGS 6505 N.E. 65th Street Seattle, WA 98115-5016 USA Phone: 206-526-6282 ext. 242 Fax: 206-526-6654 E-mail: cmconway@usgs.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tjasper <@t> copc.net Tue May 21 19:28:30 2013 From: tjasper <@t> copc.net (Thomas Jasper) Date: Tue May 21 19:28:37 2013 Subject: [Histonet] Unsubscribe Message-ID: <90354A475B420441B2A0396E5008D496D3E8C7@copc-sbs.COPC.local> From lpwenk <@t> sbcglobal.net Tue May 21 22:23:44 2013 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Tue May 21 22:23:55 2013 Subject: [Histonet] Unsubscribe In-Reply-To: <90354A475B420441B2A0396E5008D496D3E8C7@copc-sbs.COPC.local> References: <90354A475B420441B2A0396E5008D496D3E8C7@copc-sbs.COPC.local> Message-ID: <2F947124EB0742C29B3EC8EAE2AF4050@HP2010> For everyone needing to unsubscribe over the summer months while on vacation, or just because . . . keep this email handy. Go to the bottom of any email. The last line is an internet address - starts with http:// has the word mailman in it. Click on the link. Scroll to the bottom of the page in the new link. type in you email address where you receive the Histonet Click on unsubscribe. Follow any other directions. Peggy Wenk -----Original Message----- From: Thomas Jasper Sent: Tuesday, May 21, 2013 8:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Unsubscribe _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From btonkin <@t> svi.edu.au Wed May 22 00:08:19 2013 From: btonkin <@t> svi.edu.au (Brett Tonkin) Date: Wed May 22 00:08:32 2013 Subject: [Histonet] Safranin O cartilage staining In-Reply-To: <974881675.113251.1369197754104.JavaMail.root@zstore.medstv.unimelb.edu.au> Message-ID: <955140388.113496.1369199299232.JavaMail.root@zstore.medstv.unimelb.edu.au> Hi, We're having trouble staining murine articular cartilage with Safranin O. We first started using this stain last year and it was working nicely, with strong staining of both the articular cartilage and growth plate. After a while, the stain stopped working, with staining only visible in the growth plate. We replaced all solutions (including c/stain of fast green) and the staining worked. Yet again, it has stopped working, and this is only the second time the solutions have been used. Has anyone come across this before? Any help or advice would be greatly appreciated! Brett Tonkin Research Assistant Arthritis Research Laboratory Bone Cell Biology and Disease Unit St. Vincent's Institute Fitzroy, Victoria From kimulah <@t> gmail.com Wed May 22 02:05:59 2013 From: kimulah <@t> gmail.com (=?ISO-2022-JP?B?GyRCTFpCPE01PHkbKEI=?=) Date: Wed May 22 02:06:09 2013 Subject: [Histonet] Unsubscribe In-Reply-To: <2F947124EB0742C29B3EC8EAE2AF4050@HP2010> References: <90354A475B420441B2A0396E5008D496D3E8C7@copc-sbs.COPC.local> <2F947124EB0742C29B3EC8EAE2AF4050@HP2010> Message-ID: 2013/05/22 12:26 "Lee & Peggy Wenk" : For everyone needing to unsubscribe over the summer months while on vacation, or just because . . . keep this email handy. Go to the bottom of any email. The last line is an internet address - starts with http:// has the word mailman in it. Click on the link. Scroll to the bottom of the page in the new link. type in you email address where you receive the Histonet Click on unsubscribe. Follow any other directions. Peggy Wenk -----Original Message----- From: Thomas Jasper Sent: Tuesday, May 21, 2013 8:28 PM To: histonet@lists.utsouthwestern.**edu Subject: [Histonet] Unsubscribe _______________________________________________ Histonet mailing list Histonet@lists.utsouthwest... From DKBoyd <@t> chs.net Wed May 22 07:56:09 2013 From: DKBoyd <@t> chs.net (Boyd, Debbie M) Date: Wed May 22 07:56:19 2013 Subject: [Histonet] Xylene substitutes for tissue processor clean cycle In-Reply-To: <4e37847c2f0e45eb95a7f414140be263@BLUPR07MB020.namprd07.prod.outlook.com> References: <4e37847c2f0e45eb95a7f414140be263@BLUPR07MB020.namprd07.prod.outlook.com> Message-ID: <7EAFE982E328304DA6CE2B677BB76246794DEF57@TN001WEXMBX12.US.chs.net> We have two Excelsiors (had Three). We use Americlear in the cleaning cycle. We have never had issues with our cleaning cycle or the operation of our processors. Our oldest processor was 12 years old before the oven cracked and started leaking. The repair was too expensive for an older processor so we replaced it with a new one. Americlear can only be purchased through Allegiance (Baxter). -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vanessa Avalos Sent: Tuesday, May 21, 2013 8:01 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Xylene substitutes for tissue processor clean cycle I use a excelsior and was told to always use Xylene in the cleaning cycle. Since sub is not as pure as xylene ( obviously) you could be at risk of developing paraffin clogs that the sub may not get. Using one xylene in the cleaning has not effected the odor at all in the lab. Good Luck. V.Avalos ADS, INC Fax:602-277-2134 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Conway, Carla Sent: Tuesday, May 21, 2013 8:26 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Xylene substitutes for tissue processor clean cycle Hello all, The technician who does preventive maintenance on our LX-120 tissue processor recommended that we use xylene as the solvent in our clean cycle. We are considering using a xylene substitue in the clean cycle and would appreciate any recommendations. Thanks very much, Carla Conway Histology Technician Western Fisheries Research Center, USGS 6505 N.E. 65th Street Seattle, WA 98115-5016 USA Phone: 206-526-6282 ext. 242 Fax: 206-526-6654 E-mail: cmconway@usgs.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From jcox90 <@t> yahoo.com Wed May 22 08:13:54 2013 From: jcox90 <@t> yahoo.com (Jill Cox) Date: Wed May 22 08:14:09 2013 Subject: [Histonet] Looking for a Leica Bond or Dako Autostainer Message-ID: <1369228434.85371.YahooMailNeo@web161605.mail.bf1.yahoo.com> Hi Netters, We are looking for a used Leica Bond or Dako Autostainer. If anyone out there is upgrading or has one for sale please respond to this email..? Thank you, Jill From minniesann <@t> hotmail.com Wed May 22 08:55:46 2013 From: minniesann <@t> hotmail.com (Maribel Santiago) Date: Wed May 22 08:55:51 2013 Subject: [Histonet] Oven recommendations Message-ID: Hi All, I had just received a letter from Boekel (models 107800-107801 or 107905 in either 120v or 230v) that my oven can not be used to melt wax of any kind. as this is our bread and butter and need some suggestions to what kind or brand is used that is good for us (histologists). I need a little one since I'm in research and I don't need a big one due to the lack of volume. Does anyone have suggestions or can point me in the right direction, Please? Thanks so much and have a great day!!!Minnie From wdesalvo.cac <@t> outlook.com Wed May 22 09:08:03 2013 From: wdesalvo.cac <@t> outlook.com (WILLIAM DESALVO) Date: Wed May 22 09:08:07 2013 Subject: [Histonet] Oven recommendations In-Reply-To: References: Message-ID: Since you state you are in reasearch, consider air drying your slides. You only need to remove the water between the paraffin section and the glass slide to allow adhesion of the proteins in the tissue sample to the glass. Melting the paraffin in not necessary, your deparaffinization steps in the routine and special staining protocols will adequately remove the paraffin from the tissue section. Using an oven that was not designed to "melt" paraffin off the glass slide can be very hazardous. The parafin can drop don into the heating elementsand cause an ignition and fire. William DeSalvo, BS HTL(ASCP) Production Manager-Anatomic Pathology Chair, NSH Quality Management Committee Owner/Consultant, Collaborative Advantage Consulting > From: minniesann@hotmail.com > To: histonet@lists.utsouthwestern.edu > Date: Wed, 22 May 2013 13:55:46 +0000 > Subject: [Histonet] Oven recommendations > > > > Hi All, > I had just received a letter from Boekel (models 107800-107801 or 107905 in either 120v or 230v) that my oven can not be used to melt wax of any kind. as this is our bread and butter and need some suggestions to what kind or brand is used that is good for us (histologists). I need a little one since I'm in research and I don't need a big one due to the lack of volume. Does anyone have suggestions or can point me in the right direction, Please? > Thanks so much and have a great day!!!Minnie > > > > > > > > > > > > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Wed May 22 09:14:26 2013 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Wed May 22 09:14:29 2013 Subject: [Histonet] Safranin O cartilage staining In-Reply-To: <955140388.113496.1369199299232.JavaMail.root@zstore.medstv.unimelb.edu.au> References: <974881675.113251.1369197754104.JavaMail.root@zstore.medstv.unimelb.edu.au> <955140388.113496.1369199299232.JavaMail.root@zstore.medstv.unimelb.edu.au> Message-ID: <14E2C6176416974295479C64A11CB9AE016411AC2DF4@SBS2K8.premierlab.local> Brett Safranin O can be a bit tricky at times. There are a few tips that we have used through the years. 1. Fresh reagents are key. 2. For proteoglycan staining you can cut the sections a bit thicker and get better staining we typically cut the joint sections for Saf O at 6 to 7 microns in thickness, there are papers out there that recommend up to 8 microns in thickness. 3. Limit excess time in decal for some reason this particular stain may not work as well if the samples are in decal for an extended period of time, we have not seen this with toluidine blue which is another stain for proteoglycan. 4. We increase times in the safranin O reagent on occasion for the murine joints. Good Luck Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Laboratory Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 Work (303) 682-3949 Fax (303) 682-9060 Cell (303) 881-0763 liz@premierlab.com www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Brett Tonkin Sent: Tuesday, May 21, 2013 11:08 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Safranin O cartilage staining Hi, We're having trouble staining murine articular cartilage with Safranin O. We first started using this stain last year and it was working nicely, with strong staining of both the articular cartilage and growth plate. After a while, the stain stopped working, with staining only visible in the growth plate. We replaced all solutions (including c/stain of fast green) and the staining worked. Yet again, it has stopped working, and this is only the second time the solutions have been used. Has anyone come across this before? Any help or advice would be greatly appreciated! Brett Tonkin Research Assistant Arthritis Research Laboratory Bone Cell Biology and Disease Unit St. Vincent's Institute Fitzroy, Victoria _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mhale <@t> MiracaLS.com Wed May 22 11:08:23 2013 From: mhale <@t> MiracaLS.com (Hale, Meredith) Date: Wed May 22 11:08:28 2013 Subject: [Histonet] Miraca Life Sciences Position's in KY Message-ID: <0E828EC51C7CC445A51E53F81B64E8C7269E5B@s-irv-exchmb.PathologyPartners.intranet> Great opportunity for Histotechnician's in Crestview Hills, KY! Miraca Life Sciences is looking to fill 2 Full Time Histologist positions in Northern Kentucky. The position will be with Miraca Life Sciences and will include benefits. The candidates must meet and have documentation to support the following requirements: * Meet CLIA Grossing Requirements : CFR 493.1489, http://wwwn.cdc.gov/clia/regs/toc.aspx/ ,prior experience grossing GI specimens * Supervisor experience preferred * HT/HTL ASCP Certified * Experience with CLIA and CAP * Experience writing and maintaining policies and procedures * Prior laboratory start up experience is preferred * Ability to work independently Duties include: * Grossing * Embedding * Microtomy * Staining; routine and special stains only * Maintain supply orders and laboratory budget * Ability to be flexible and take on additional duties' as needed * Ability to work independently * Maintenance of laboratory for inspections * Maintenance of quality records Interested applicants should contact Meredith Hale; phone 214-596-2219 or through email mhale@miracals.com Meredith Hale HT (ASCP)cm Director External Sales Support Miraca Life Sciences 6655 North MacArthur Blvd. Irving , Texas 75039 Office: 214-596-2219 Cell: 469-648-8253 Fax: 1-866-688-3280 mhale@miracals.com> From kmendell <@t> goldbergmd.net Wed May 22 11:39:40 2013 From: kmendell <@t> goldbergmd.net (MaryK Mendell) Date: Wed May 22 11:39:45 2013 Subject: [Histonet] Oven recommendations In-Reply-To: References: , Message-ID: <8F7FA50134ED99419B6CD9A978CDD7A556188A0A@EXMBX104A.mmeprod.cbeyond> Minnie This is what I have used for years as a dryer. You can go onto the TBS website : SD-II-120 Slide Dryer II, forced air, holds 2 conventional slide stainer racks, overheat protection switch, ambient to 75?C. Specifications: 100-120VAC, 50/60Hz, 2A; SD-II-220: 220-240VAC, 50/60Hz, 2A. WxDxH: (11x10x5) (26x28x13)(in)(cm). Weight: 11lbs, 5kg As far as fire goes, I do know someone that happen to and it could have been a disaster had it not been for quick thinking on the techs part. Kate Mendell Histopathology/Lab Manager HOWARD S. GOLDBERG, M.D., INC 990 Paradise Road Swampscott, MA 01907 TEL: 781.595.0151 FAX: 781.592.6780 kmendell@goldbergmd.net www.cosmesticdermcenter.com PRIVACY NOTICE: This e-mail message may contain confidential patient or other information belonging to the sender that is legally privileged. This information is intended only for the use of the individual or authorized entity named above. The authorized recipient of this patient or other confidential information is prohibited from disclosing the information to any other party. If you have received this message in error, please notify the sender immediately and delete. Please keep any information you may have viewed confidential. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of WILLIAM DESALVO [wdesalvo.cac@outlook.com] Sent: Wednesday, May 22, 2013 10:08 AM To: Maribel Santiago; histonet Subject: RE: [Histonet] Oven recommendations Since you state you are in reasearch, consider air drying your slides. You only need to remove the water between the paraffin section and the glass slide to allow adhesion of the proteins in the tissue sample to the glass. Melting the paraffin in not necessary, your deparaffinization steps in the routine and special staining protocols will adequately remove the paraffin from the tissue section. Using an oven that was not designed to "melt" paraffin off the glass slide can be very hazardous. The parafin can drop don into the heating elementsand cause an ignition and fire. William DeSalvo, BS HTL(ASCP) Production Manager-Anatomic Pathology Chair, NSH Quality Management Committee Owner/Consultant, Collaborative Advantage Consulting > From: minniesann@hotmail.com > To: histonet@lists.utsouthwestern.edu > Date: Wed, 22 May 2013 13:55:46 +0000 > Subject: [Histonet] Oven recommendations > > > > Hi All, > I had just received a letter from Boekel (models 107800-107801 or 107905 in either 120v or 230v) that my oven can not be used to melt wax of any kind. as this is our bread and butter and need some suggestions to what kind or brand is used that is good for us (histologists). I need a little one since I'm in research and I don't need a big one due to the lack of volume. Does anyone have suggestions or can point me in the right direction, Please? > Thanks so much and have a great day!!!Minnie > > > > > > > > > > > > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jamie.erickson <@t> abbvie.com Wed May 22 14:27:07 2013 From: jamie.erickson <@t> abbvie.com (Erickson, Jamie E) Date: Wed May 22 14:27:12 2013 Subject: [Histonet] Looking for mouse antibody of LTa for use in IHC Message-ID: <8B946A68A8F3534A99CC493DEFB49B101322B2AF@WM10002P.oneabbott.com> Hello Histonetters, Does anyone know of a antibody to mouse LTa (Lymphotoxin alpha (TNF Superfamily, Member 1) (LTA)) that works in IHC. A colleague is pulling her hair out trying various clones in formalin and frozen sections of mouse spleen but with no luck, please help her.. Is there one that works or is she chasing a ghost... Thanks for any help you can provide.. Jamie Jamie Erickson Abbvie laboratories Scientist II HTL (ASCP),MS From Wanda.Smith <@t> HCAhealthcare.com Wed May 22 15:57:46 2013 From: Wanda.Smith <@t> HCAhealthcare.com (Wanda.Smith@HCAhealthcare.com) Date: Wed May 22 15:57:58 2013 Subject: [Histonet] CHARLESTON, SC HISTOTECHS-I need a PRN HT Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BA27DA0E8CB4@NADCWPMSGCMS03.hca.corpad.net> I have a PRN Ht position available for early morning fill-ins and/or set days during the week. Upcoming maternity leave also. 4:00 am until you have to go to your other job or work part time for us. GREAT crew and Pathologist to work for and opportunities to multitask, i.e., embedding, cutting, labeling, Special Stains and IHC. Position is posted on the TridentHealthSystem.com website, position #11816. Call or email me with questions. Thanks, Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. From krista.sider <@t> utoronto.ca Wed May 22 16:19:05 2013 From: krista.sider <@t> utoronto.ca (Krista Sider) Date: Wed May 22 16:19:11 2013 Subject: [Histonet] Movat Pentachrome - Can't Remove Woodstain Scarlet-Acid Fuchsin from Collagen In-Reply-To: <000001ce4e1e$25ca3f80$715ebe80$@gmx.at> References: <000001ce4e1e$25ca3f80$715ebe80$@gmx.at> Message-ID: Thank you all very much for you help. I really appreciate it. Gudrun: I tried the PTA before the WSAF and it worked beautifully (1 min PTA, dH2O, 5 min WSAF, dH20, AA, 10 min PTA). Red muscle and clean collagen.Thank-you Liz: I didn't get to trying the 60*C Bouin's but I will make a note for next time I am optimizing. Thanks again Krista On Sat, May 11, 2013 at 4:04 AM, Gudrun Lang wrote: > Try to stain first in PTA/PMA solution to impregnate the collagen fibers - > perhaps testing with different times. Then follow up with red stain and the > usual procedure. > > We use a stain called SFOG, that first impregnates 2 min with PMA and > afterwards with the mixture of Chromotrop, Acidfuchsin and Anilinblue for > 10 > min. The longer we do the Polyacid-step the more intensive are the fibres > and less intensive is the cytoplasma. > I think, if after this trial the collagen is still red, that there are > binding-sites in the collagen, that can't be influenced. Maybe acidfuchsin > is here the main partner and a pure solution of Scarlet Red may help. > > Gudrun Lang > > -----Urspr?ngliche Nachricht----- > Von: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Krista > Sider > Gesendet: Freitag, 10. Mai 2013 23:13 > An: histonet@lists.utsouthwestern.edu > Betreff: [Histonet] Movat Pentachrome - Can?t Remove Woodstain Scarlet-Acid > Fuchsin from Collagen > > Hello All, > > I have successfully stained porcine and mouse paraffin embedded heart > tissues with Movat?s Pentachrome (MP), using EMS? MP solutions and protocol > (http://www.emsdiasum.com/microscopy/technical/datasheet/26385.aspx), with > some optimization on times. > > However, now I am working with archival human tissue that has been fixed > for > much longer and is much older than my other samples (Human Aorta & Aortic > Valve, formalin fixed 2-6 months or 2-4 years, embedded in paraffin and > stored at RT for ~6 years). I cannot seem to get the Woodstain Scarlet-Acid > Fuchsin (WSAF) to pull out of the collagen with the 5% Phosphotungstic Acid > (PA) or 1% Phosphomolybdic acid (PM) Acid. I have tried increasing the > Bouin?s initial fixation (max 2 hrs 50*C), diluting the standard WSAF > solution by 50% (1 dip) and taking the PA or PM up to 1 hr, yet there is > always a lot of red still left in the collagen, in some regions as strong > as > the muscle (which I am sure are not muscle or dense cells). I see virtually > no change with increased PA or PM time. As I have aorta in my samples I > can?t just leave out the muscle stain. > > I would be very grateful for your insights into anything I could try to get > clean collagen and stained muscle in the MP stain. Why might my method not > be working? Is there something I can substitute for the WSAF that might be > appropriate? > > Thank you very much for you help, > > Krista > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From cforster <@t> umn.edu Wed May 22 18:01:17 2013 From: cforster <@t> umn.edu (Colleen Forster) Date: Wed May 22 18:01:23 2013 Subject: [Histonet] CD200(OX2) that works in FFPE mouse samples Message-ID: <519D4E3D.8090805@umn.edu> To all the histonetters, IS anyone doing CD200 staining in FFPE mouse samples with good results? If so, please share your information..... Thanks in advance...your help is always appreciated!! Colleen Forster U of MN From jcox90 <@t> yahoo.com Thu May 23 10:26:56 2013 From: jcox90 <@t> yahoo.com (Jill Cox) Date: Thu May 23 10:27:00 2013 Subject: [Histonet] Microscope Vendor in AZ? Message-ID: <1369322816.24144.YahooMailNeo@web161605.mail.bf1.yahoo.com> Hi Netters, Does anyone know of a Microscope sales rep in AZ? Looking for an Olympus BX40 or BX41. We need one asap.. Thanks! From PAGE.BALUCH <@t> asu.edu Thu May 23 11:14:13 2013 From: PAGE.BALUCH <@t> asu.edu (Debra Baluch) Date: Thu May 23 11:14:22 2013 Subject: [Histonet] Microscope Vendor in AZ? In-Reply-To: <1369322816.24144.YahooMailNeo@web161605.mail.bf1.yahoo.com> References: <1369322816.24144.YahooMailNeo@web161605.mail.bf1.yahoo.com> Message-ID: <0BA26BDE2F5A9247B37581A1045F65E41ECE10AD@exmbt01.asurite.ad.asu.edu> The Olympus vendor rep for the Arizona region is Myron McKenzie (myron.mckenzie@olympus.com). Page D. Page Baluch, Ph.D. W.M. Keck Bioimaging Laboratory Arizona State University/School of Life Sciences 480-727-0725 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jill Cox Sent: Thursday, May 23, 2013 8:27 AM To: Histonet@Lists. Edu Subject: [Histonet] Microscope Vendor in AZ? Hi Netters, Does anyone know of a Microscope sales rep in AZ? Looking for an Olympus BX40 or BX41. We need one asap.. Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rooster3801 <@t> yahoo.com Thu May 23 12:13:08 2013 From: rooster3801 <@t> yahoo.com (Rooster C) Date: Thu May 23 12:13:13 2013 Subject: [Histonet] PT lab aide Message-ID: <1369329188.81579.YahooMailNeo@web161801.mail.bf1.yahoo.com> Hello fellow histoneters, ???? I am the lab manager of a small private dermatopathology lab in Savannah GA, and I am looking for some part time help.? I am looking for a lab aide to help with routine H&E's and machinery maintenence?Monday through Friday early AM.?? Wages negotiable, LAC not necessary but helpful, experience with dermatopathology a plus.? ? Thank You, Christopher Copeland HT(ASCP) Laboratory Manager Savannah Skin Pathology From tony.auge <@t> gmail.com Thu May 23 14:50:16 2013 From: tony.auge <@t> gmail.com (Tony Auge) Date: Thu May 23 14:50:22 2013 Subject: [Histonet] MSH2 and MSH6 validation Message-ID: Hello, I am looking to incorporate the MSI IHC panel into my lab and need to validate the antibodies. I have positive tissue for MLH-1 and PMS2 and have validated the performance of those antibodies but I can't find any positive tissue for MSH2 and MSH6. Would anyone be willing to help me out with where I might be able to procure some positive tissue? Thank you, -- Tony Auge HTL (ASCP) Cell: (651) 373-4768 Email: tony.auge@gmail.com From tkngflght <@t> yahoo.com Thu May 23 17:46:12 2013 From: tkngflght <@t> yahoo.com (Cheryl Kerry) Date: Thu May 23 17:46:17 2013 Subject: [Histonet] Cell block prep procedures Message-ID: Hey guys-- looking to collect as many different cell block prep procedures as I can find. Want the best outcome for my lab! What do you have/ what have you do e that really works? Cheryl Eric--I cleaned off the thread! You should be so proud !!! Sent from my iPhone > From Fawaz.Zouabi <@t> sswahs.nsw.gov.au Thu May 23 23:29:18 2013 From: Fawaz.Zouabi <@t> sswahs.nsw.gov.au (Fawaz Zouabi) Date: Thu May 23 23:29:39 2013 Subject: [Histonet] Gram stain Message-ID: <04F39AE5B71E6D4CAAB4E1F2CB5393F818E135@sswlivma02.intra.swsahs.nsw.gov.au> Hi histonetters does any one have a good working method for GRAM stain ??? I used mod brown's variation and my Neg bact still does not stain red. Fawaz Zouabi Histo-Technologist Department of Forensic Medicine Glebe NSW Forensic & Analytical Science Service - FASS P O Box 90 Glebe NSW 2037 | Tel +612 8584 7842 | Fax +612 95664573 _____________________________________________________________________ This email has been scanned for the Sydney & South Western Sydney Local Health Districts by the MessageLabs Email Security System. Sydney & South Western Sydney Local Health Districts regularly monitor email and attachments to ensure compliance with the NSW Ministry of Health's Electronic Messaging Policy. From JMacDonald <@t> mtsac.edu Thu May 23 23:33:57 2013 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Thu May 23 23:34:04 2013 Subject: [Histonet] Gram stain In-Reply-To: <04F39AE5B71E6D4CAAB4E1F2CB5393F818E135@sswlivma02.intra.swsahs.nsw.gov.au> References: <04F39AE5B71E6D4CAAB4E1F2CB5393F818E135@sswlivma02.intra.swsahs.nsw.gov.au> Message-ID: We use a Twort stain with my students. They have been doing the gram stain the last couple of days and getting great results. I will look for the entire procedure. From: "Fawaz Zouabi" To: Date: 05/23/2013 09:32 PM Subject: [Histonet] Gram stain Sent by: histonet-bounces@lists.utsouthwestern.edu Hi histonetters does any one have a good working method for GRAM stain ??? I used mod brown's variation and my Neg bact still does not stain red. Fawaz Zouabi Histo-Technologist Department of Forensic Medicine Glebe NSW Forensic & Analytical Science Service - FASS P O Box 90 Glebe NSW 2037 | Tel +612 8584 7842 | Fax +612 95664573 _____________________________________________________________________ This email has been scanned for the Sydney & South Western Sydney Local Health Districts by the MessageLabs Email Security System. Sydney & South Western Sydney Local Health Districts regularly monitor email and attachments to ensure compliance with the NSW Ministry of Health's Electronic Messaging Policy. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Fri May 24 07:10:39 2013 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Fri May 24 07:10:43 2013 Subject: [Histonet] MSH2 and MSH6 validation In-Reply-To: References: Message-ID: I can probably help, but it will be a couple of months out. Joelle Weaver MAOM, HTL (ASCP) QIHC > Date: Thu, 23 May 2013 12:50:16 -0700 > From: tony.auge@gmail.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] MSH2 and MSH6 validation > > Hello, > > I am looking to incorporate the MSI IHC panel into my lab and need to > validate the antibodies. I have positive tissue for MLH-1 and PMS2 and > have validated the performance of those antibodies but I can't find any > positive tissue for MSH2 and MSH6. Would anyone be willing to help me out > with where I might be able to procure some positive tissue? > > Thank you, > > -- > > Tony Auge HTL (ASCP) > Cell: (651) 373-4768 > Email: tony.auge@gmail.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From paw555 <@t> yahoo.com Fri May 24 08:55:07 2013 From: paw555 <@t> yahoo.com (pam plumlee) Date: Fri May 24 08:55:11 2013 Subject: [Histonet] Working in a Lab alone Message-ID: <1369403707.80639.YahooMailNeo@web120906.mail.ne1.yahoo.com> Good morning:? I'm trying to help out my CLS coworkers...they have been asked to work Saturdays-by themselves-1 person alone in the lab for approx. 4-5 hours.? They are a bit worried by the security factor-although we are located in a kind of remote industrial area, the building has card key locked doors.? My concern is working in the lab alone.? We have many freezers, fridges and other water sources that may present a slip risk.? Does anyone know if there are state, CAP, CLIA guidelines, preventative rules about this matter.? We are located in California.? Any help, suggestions appreciated.? Pam at BioT. From rjbuesa <@t> yahoo.com Fri May 24 09:09:20 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri May 24 09:09:27 2013 Subject: [Histonet] Working in a Lab alone In-Reply-To: <1369403707.80639.YahooMailNeo@web120906.mail.ne1.yahoo.com> References: <1369403707.80639.YahooMailNeo@web120906.mail.ne1.yahoo.com> Message-ID: <1369404560.65828.YahooMailNeo@web163104.mail.bf1.yahoo.com> There are no prohibitions (if that is what you mean by "regulations") about working by yourself in a lab. That is totally an administrative decision usually guided by the need of not letting tissues in hot paraffin, or reducing Monday's complement or any other reason. Intrinsically it is not dangerous unless the worker has a medical condition in which case the worker should state that to be excused. Other than that, there is no special dangerous circumstances for somebody to work alone in a lab. If you do not like it or cannot just say it. That is your prerogative regardless of the existence or not of any regulation on the subject. Personally I love?it and did it for some time when I started in this profession. Ren? J. From: pam plumlee To: "Histonet@lists.utsouthwestern.edu" Sent: Friday, May 24, 2013 9:55 AM Subject: [Histonet] Working in a Lab alone Good morning:? I'm trying to help out my CLS coworkers...they have been asked to work Saturdays-by themselves-1 person alone in the lab for approx. 4-5 hours.? They are a bit worried by the security factor-although we are located in a kind of remote industrial area, the building has card key locked doors.? My concern is working in the lab alone.? We have many freezers, fridges and other water sources that may present a slip risk.? Does anyone know if there are state, CAP, CLIA guidelines, preventative rules about this matter.? We are located in California.? Any help, suggestions appreciated.? Pam at BioT. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Fri May 24 09:12:22 2013 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Fri May 24 09:12:27 2013 Subject: [Histonet] Working in a Lab alone In-Reply-To: <1369403707.80639.YahooMailNeo@web120906.mail.ne1.yahoo.com> References: <1369403707.80639.YahooMailNeo@web120906.mail.ne1.yahoo.com> Message-ID: <14E2C6176416974295479C64A11CB9AE016411AC2E3E@SBS2K8.premierlab.local> Pam I don't believe that they are any reqs regarding this, but there is a tremendous amount of information on the web associated with this topic (working alone in the lab). We have a policy here that no one can work in the lab alone, but we instituted that ourselves and we are a small business. This has been addressed at lot at universities when individuals are in the lab at all hours of the day by themselves and if something happens there is really no one else there to help. I believe I have some documents in my files that I can forward onto you if you like but I did not see any regulations specifically and policies varied from institution to institution. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Laboratory Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 Work (303) 682-3949 Fax (303) 682-9060 Cell (303) 881-0763 liz@premierlab.com www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of pam plumlee Sent: Friday, May 24, 2013 7:55 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Working in a Lab alone Good morning: I'm trying to help out my CLS coworkers...they have been asked to work Saturdays-by themselves-1 person alone in the lab for approx. 4-5 hours. They are a bit worried by the security factor-although we are located in a kind of remote industrial area, the building has card key locked doors. My concern is working in the lab alone. We have many freezers, fridges and other water sources that may present a slip risk. Does anyone know if there are state, CAP, CLIA guidelines, preventative rules about this matter. We are located in California. Any help, suggestions appreciated. Pam at BioT. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vtobias <@t> uw.edu Fri May 24 09:19:29 2013 From: vtobias <@t> uw.edu (Victor A. Tobias) Date: Fri May 24 09:24:49 2013 Subject: [Histonet] Working in a Lab alone In-Reply-To: <1369403707.80639.YahooMailNeo@web120906.mail.ne1.yahoo.com> References: <1369403707.80639.YahooMailNeo@web120906.mail.ne1.yahoo.com> Message-ID: Pam, Sounds like the bigger issue is to clean up the unsafe work conditions. Victor Victor Tobias HT(ASCP) Clinical Applications Analyst Harborview Medical Center Dept of Pathology Room NJB244 Seattle, WA 98104 vtobias@u.washington.edu 206-744-2735 206-744-8240 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of pam plumlee Sent: Friday, May 24, 2013 6:55 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Working in a Lab alone Good morning:? I'm trying to help out my CLS coworkers...they have been asked to work Saturdays-by themselves-1 person alone in the lab for approx. 4-5 hours.? They are a bit worried by the security factor-although we are located in a kind of remote industrial area, the building has card key locked doors.? My concern is working in the lab alone.? We have many freezers, fridges and other water sources that may present a slip risk.? Does anyone know if there are state, CAP, CLIA guidelines, preventative rules about this matter.? We are located in California.? Any help, suggestions appreciated.? Pam at BioT. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From asmith <@t> mail.barry.edu Fri May 24 10:50:25 2013 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Fri May 24 10:50:39 2013 Subject: [Histonet] Working in a Lab alone In-Reply-To: <14E2C6176416974295479C64A11CB9AE016411AC2E3E@SBS2K8.premierlab.local> References: <1369403707.80639.YahooMailNeo@web120906.mail.ne1.yahoo.com> <14E2C6176416974295479C64A11CB9AE016411AC2E3E@SBS2K8.premierlab.local> Message-ID: <93D1C752AF965C43AE4C84D6D45CFC2FDFD748@BL2PRD0710MB373.namprd07.prod.outlook.com> Normally, I work alone. Sometimes a graduate student works with me. In a few cases, where special hazards are involved (e.g. lithium aluminum hydride, diazomethane), I will work only if I am alone in my lab so that I will be the only one injured AND someone who could help is present in an adjacent lab. Allen A. Smith,Ph.D. Professor of Anatomy Barry University School of Podiatric Medicine -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala Sent: Friday, May 24, 2013 10:12 AM To: pam plumlee; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Working in a Lab alone Pam I don't believe that they are any reqs regarding this, but there is a tremendous amount of information on the web associated with this topic (working alone in the lab). We have a policy here that no one can work in the lab alone, but we instituted that ourselves and we are a small business. This has been addressed at lot at universities when individuals are in the lab at all hours of the day by themselves and if something happens there is really no one else there to help. I believe I have some documents in my files that I can forward onto you if you like but I did not see any regulations specifically and policies varied from institution to institution. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Laboratory Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 Work (303) 682-3949 Fax (303) 682-9060 Cell (303) 881-0763 liz@premierlab.com www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of pam plumlee Sent: Friday, May 24, 2013 7:55 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Working in a Lab alone Good morning: I'm trying to help out my CLS coworkers...they have been asked to work Saturdays-by themselves-1 person alone in the lab for approx. 4-5 hours. They are a bit worried by the security factor-although we are located in a kind of remote industrial area, the building has card key locked doors. My concern is working in the lab alone. We have many freezers, fridges and other water sources that may present a slip risk. Does anyone know if there are state, CAP, CLIA guidelines, preventative rules about this matter. We are located in California. Any help, suggestions appreciated. Pam at BioT. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Fri May 24 12:01:15 2013 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Fri May 24 12:01:23 2013 Subject: [Histonet] Working in a Lab alone In-Reply-To: <93D1C752AF965C43AE4C84D6D45CFC2FDFD748@BL2PRD0710MB373.namprd07.prod.outlook.com> References: <1369403707.80639.YahooMailNeo@web120906.mail.ne1.yahoo.com> <14E2C6176416974295479C64A11CB9AE016411AC2E3E@SBS2K8.premierlab.local> <93D1C752AF965C43AE4C84D6D45CFC2FDFD748@BL2PRD0710MB373.namprd07.prod.outlook.com> Message-ID: <761E2B5697F795489C8710BCC72141FF0818A6@ex07.net.ucsf.edu> These days you could install webcams to cover the lab and have someone monitor it. Tim Morken -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Smith, Allen Sent: Friday, May 24, 2013 8:50 AM To: Elizabeth Chlipala Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Working in a Lab alone Normally, I work alone. Sometimes a graduate student works with me. In a few cases, where special hazards are involved (e.g. lithium aluminum hydride, diazomethane), I will work only if I am alone in my lab so that I will be the only one injured AND someone who could help is present in an adjacent lab. Allen A. Smith,Ph.D. Professor of Anatomy Barry University School of Podiatric Medicine -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala Sent: Friday, May 24, 2013 10:12 AM To: pam plumlee; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Working in a Lab alone Pam I don't believe that they are any reqs regarding this, but there is a tremendous amount of information on the web associated with this topic (working alone in the lab). We have a policy here that no one can work in the lab alone, but we instituted that ourselves and we are a small business. This has been addressed at lot at universities when individuals are in the lab at all hours of the day by themselves and if something happens there is really no one else there to help. I believe I have some documents in my files that I can forward onto you if you like but I did not see any regulations specifically and policies varied from institution to institution. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Laboratory Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 Work (303) 682-3949 Fax (303) 682-9060 Cell (303) 881-0763 liz@premierlab.com www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of pam plumlee Sent: Friday, May 24, 2013 7:55 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Working in a Lab alone Good morning: I'm trying to help out my CLS coworkers...they have been asked to work Saturdays-by themselves-1 person alone in the lab for approx. 4-5 hours. They are a bit worried by the security factor-although we are located in a kind of remote industrial area, the building has card key locked doors. My concern is working in the lab alone. We have many freezers, fridges and other water sources that may present a slip risk. Does anyone know if there are state, CAP, CLIA guidelines, preventative rules about this matter. We are located in California. Any help, suggestions appreciated. Pam at BioT. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Mon May 27 10:37:04 2013 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Mon May 27 10:38:17 2013 Subject: [Histonet] MDM2 on lipsarcoma IHC Message-ID: <001e01ce5af0$0b57d700$22078500$@gmx.at> Hi all! We try to improve our MDM2-staining and purchased a new antibody. This one stains all nuclei in liposarcoma but also in normal surrounding tissue and lipoma positiv. On the Protein-Atlas-site MDM2 is described with "Ubiquitous nuclear expression". On the other hand I read, that well differentiated liposarcoma are MDM2 negativ, dedifferentiated liposarcoma are positiv. Most likely the point is "overexpression" of MDM2 protein due to gene-amplification. Maybe we have to "turn down" our protocol to detect only overexpressed MDM2. Has anyone practical experience with this marker? Can help me? http://www.proteinatlas.org/ENSG00000135679 Bye and thanks in advance Gudrun Lang From marktarango <@t> gmail.com Mon May 27 12:54:00 2013 From: marktarango <@t> gmail.com (Mark Tarango) Date: Mon May 27 12:54:05 2013 Subject: [Histonet] MDM2 on lipsarcoma IHC In-Reply-To: <001e01ce5af0$0b57d700$22078500$@gmx.at> References: <001e01ce5af0$0b57d700$22078500$@gmx.at> Message-ID: Hi Gudrun, We only do FISH for MDM2 but it is positive for gene amplification in both well differentiated liposarcomas and dedifferentiated liposarcomas. Lipomas and other sarcomas should be negative as far as I've seen. I haven't worked with the antibody or seen that staining, but I'd guess that you are right and the protocol needs to be brought down. That link you have in your e-mail seems to imply that the antibody stains nearly all nuclei though. Maybe the antibody isn't just staining the overexpressed cells... but all of them? "Ubiquitous nuclear expression" doesn't sound very specific for the cells that are positive for gene amplification. Mark On Mon, May 27, 2013 at 8:37 AM, Gudrun Lang wrote: > Hi all! > > We try to improve our MDM2-staining and purchased a new antibody. This one > stains all nuclei in liposarcoma but also in normal surrounding tissue and > lipoma positiv. > > On the Protein-Atlas-site MDM2 is described with "Ubiquitous nuclear > expression". On the other hand I read, that well differentiated liposarcoma > are MDM2 negativ, dedifferentiated liposarcoma are positiv. > > > > Most likely the point is "overexpression" of MDM2 protein due to > gene-amplification. Maybe we have to "turn down" our protocol to detect > only > overexpressed MDM2. > > > > Has anyone practical experience with this marker? Can help me? > > > > http://www.proteinatlas.org/ENSG00000135679 > > > > > > Bye and thanks in advance > > Gudrun Lang > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From rgrow <@t> bmnet.com Mon May 27 15:01:52 2013 From: rgrow <@t> bmnet.com (rgrow@bmnet.com) Date: Mon May 27 15:01:58 2013 Subject: [Histonet] Renee Grow is out of the office. Message-ID: I will be out of the office starting 05/27/2013 and will not return until 06/06/2013. I will respond to your message when I return. ________________________________ This communication may contain protected health information (PHI) that is legally protected from inappropriate disclosure by the Privacy Standards of the Health Insurance Portability and Accountability Act (HIPAA) and relevant Tennessee Laws. If you are not the intended recipient, please note that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this message in error, you should notify the sender immediately by telephone or by return e-mail and delete this message from your computer. Direct questions to the Blount Memorial Hospital Privacy Officer at 865-977-4675. From krista.sider <@t> utoronto.ca Mon May 27 16:28:06 2013 From: krista.sider <@t> utoronto.ca (Krista Sider) Date: Mon May 27 16:28:12 2013 Subject: [Histonet] Movat Pentachrome - Need to enhance Alcian Blue Message-ID: Hi All, I had wonderful help from this site for my Woodstain Scarlet-Acid Fuchsin binding collagen problem. Now that this is fixed I have realized that the Alcian Blue is not staining my excessively fixed samples (Human Aorta & Aortic Valve, formalin fixed 2-4 years, embedded in paraffin and stored at RT for ~6 years). I have some samples fixed for only 2-6 months and these are fine but the samples fixed for 2-4 years do not uptake the Alcian Blue. I have taken it up to 1 hr in the 1% Alcian Blue solution followed by 10 min in 56*C Alkaline Alcohol solution. I do a 1hr Bouin's 50*C to start (I have also tried Bouin's at 60*C). Any ideas on how I can get the Alcian Blue to stain these excessively fixed samples or possibly a substitute? I need to see the proteoglycans/GAGs. Thank you Krista From talulahgosh <@t> gmail.com Tue May 28 08:39:18 2013 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Tue May 28 08:39:25 2013 Subject: [Histonet] antibodies online Message-ID: Hello Histonetters! What search engines do you use to find antibodies that are available commercially? I use biocompare, but I'm not sure if that's the best site. I would just google it, but that brings up waaaay too many protocols and papers (though the papers are sometimes a good source as well) Searching individual companies sometimes helps, but I've found that anti-chick antibodies are usually sold by small companies. Emily "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." -Chuck Palahniuk, "Haunted" From liz <@t> premierlab.com Tue May 28 08:56:51 2013 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Tue May 28 08:56:57 2013 Subject: [Histonet] antibodies online In-Reply-To: References: Message-ID: <14E2C6176416974295479C64A11CB9AE016411AC2E5A@SBS2K8.premierlab.local> Emily We use biocompare, google, pub med and human protein atlas. I always try to look at some papers if I can, with papers you can review materials and methods and also review images, etc. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Laboratory Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 Work (303) 682-3949 Fax (303) 682-9060 Cell (303) 881-0763 liz@premierlab.com www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Tuesday, May 28, 2013 7:39 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] antibodies online Hello Histonetters! What search engines do you use to find antibodies that are available commercially? I use biocompare, but I'm not sure if that's the best site. I would just google it, but that brings up waaaay too many protocols and papers (though the papers are sometimes a good source as well) Searching individual companies sometimes helps, but I've found that anti-chick antibodies are usually sold by small companies. Emily "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." -Chuck Palahniuk, "Haunted" _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Tue May 28 09:08:18 2013 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Tue May 28 09:09:31 2013 Subject: AW: [Histonet] Movat Pentachrome - Need to enhance Alcian Blue In-Reply-To: References: Message-ID: <001d01ce5bac$ce5c48a0$6b14d9e0$@gmx.at> Hi Krista, Maybe the bad stainability is more due to oxidation by air of the old block-surface than due to fixation. A deeper slide for comparison would show. I don't know what the alkaline alcohol is good for ? Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Krista Sider Gesendet: Montag, 27. Mai 2013 23:28 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Movat Pentachrome - Need to enhance Alcian Blue Hi All, I had wonderful help from this site for my Woodstain Scarlet-Acid Fuchsin binding collagen problem. Now that this is fixed I have realized that the Alcian Blue is not staining my excessively fixed samples (Human Aorta & Aortic Valve, formalin fixed 2-4 years, embedded in paraffin and stored at RT for ~6 years). I have some samples fixed for only 2-6 months and these are fine but the samples fixed for 2-4 years do not uptake the Alcian Blue. I have taken it up to 1 hr in the 1% Alcian Blue solution followed by 10 min in 56*C Alkaline Alcohol solution. I do a 1hr Bouin's 50*C to start (I have also tried Bouin's at 60*C). Any ideas on how I can get the Alcian Blue to stain these excessively fixed samples or possibly a substitute? I need to see the proteoglycans/GAGs. Thank you Krista _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brett_connolly <@t> merck.com Tue May 28 09:29:35 2013 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Tue May 28 09:29:46 2013 Subject: [Histonet] antibodies online In-Reply-To: References: Message-ID: I find that the individual antibody search engines I use each provide info from select companies so I generally look at several ...in addition to Biocompare, Google, Human Protein Atlas and PubMed searches. http://www.antibodyresource.com/home http://www.antibodydirectory.com/index2.php http://www.labome.com/ http://www.linscottsdirectory.com/ http://www.nordiqc.org/Default.htm and, here is one for working on murine tissue specifically, I don't think it gets updated though http://ncifrederick.cancer.gov/rtp/lasp/phl/immuno/ Brett Brett M. Connolly, Ph.D. Principal Scientist, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Tuesday, May 28, 2013 9:39 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] antibodies online Hello Histonetters! What search engines do you use to find antibodies that are available commercially? I use biocompare, but I'm not sure if that's the best site. I would just google it, but that brings up waaaay too many protocols and papers (though the papers are sometimes a good source as well) Searching individual companies sometimes helps, but I've found that anti-chick antibodies are usually sold by small companies. Emily "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." -Chuck Palahniuk, "Haunted" _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From BMolinari <@t> texasheart.org Tue May 28 10:47:19 2013 From: BMolinari <@t> texasheart.org (Molinari, Betsy) Date: Tue May 28 10:47:28 2013 Subject: [Histonet] another Movats question (paraffin) Message-ID: Hi, The Alcian Blue in my Movats is very faint or seems to completely disappear. I have been running Movats for years and this is a new problem. I have checked lot #?s everything is the same. The solution is 1% pH 2.7. The slides are in solution for 20 mins, rinse for 5 mins in tap H2O, then in alkaline alcohol (90 ml 95% 10 ml ammonium hydroxide) for 1 hr then rinsed for 10 mins. But after hematoxylin the blue is either gone or very faint. Any suggestions? Thanks, Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston , TX 77030 832-355-6524 (lab) 832-355-6812 (fax) Betsy Molinari Senior Histology Research Technician 832-355-6524 | BMolinari@texasheart.org | www.texasheart.org 6770 Bertner Ave., MC 1-283, Houston, TX 77030 [Texas Heart Institute][THI News] [THI on Facebook] [THI on Flicker] [THI on Google] [THI on Pinterest] [THI on Twitter] [THI on You Tube] Confidentiality Notice: This message may be confidential and/or privileged. If you are not the intended recipient you may not review, disseminate or copy this e-mail, its contents and/or any attachments. Please immediately notify the sender If you have received this e-mail in error and delete it from your computer system. From koellingr <@t> comcast.net Tue May 28 11:01:46 2013 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Tue May 28 11:04:11 2013 Subject: [Histonet] another Movats question (paraffin) In-Reply-To: Message-ID: <427082183.402707.1369756906652.JavaMail.root@sz0001a.emeryville.ca.mail.comcast.net> Betsy, Don't know if applicable to this situation but in the past I have on occasion in other labs noted some stain going off and after checking everything, looked at the tap water. In fact I backtracked through the water utility once to see where the water came from, was delivered and what the utility did to purify. Convinced, although never proven, that tap water was culprit on at least one or two occasions. Utility would say they used "this chemical" to purify but on occasion changed to "that chemical". Although we fall into the habit that tap water is tap water it can change from time to time (source, purification, metals eroding in old pipes, etc). Don't have to be a chemist to drink tap water in one part of US and find it completely different tasting from another part of country. Maybe not likely cause but worth considering. Ray Research Scientist UW Seattle ----- Original Message ----- From: "Betsy Molinari" To: Histonet@lists.utsouthwestern.edu Sent: Tuesday, May 28, 2013 8:47:19 AM Subject: [Histonet] another Movats question (paraffin) Hi, The Alcian Blue in my Movats is very faint or seems to completely disappear. I have been running Movats for years and this is a new problem. I have checked lot #?s everything is the same. The solution is 1% pH 2.7. The slides are in solution for 20 mins, rinse for 5 mins in tap H2O, then in alkaline alcohol (90 ml 95% 10 ml ammonium hydroxide) for 1 hr then rinsed for 10 mins. But after hematoxylin the blue is either gone or very faint. Any suggestions? Thanks, Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston , TX 77030 832-355-6524 (lab) 832-355-6812 (fax) Betsy Molinari Senior Histology Research Technician 832-355-6524 | BMolinari@texasheart.org | www.texasheart.org 6770 Bertner Ave., MC 1-283, Houston, TX 77030 [Texas Heart Institute][THI News] [THI on Facebook] [THI on Flicker] [THI on Google] [THI on Pinterest] [THI on Twitter] [THI on You Tube] Confidentiality Notice: This message may be confidential and/or privileged. If you are not the intended recipient you may not review, disseminate or copy this e-mail, its contents and/or any attachments. Please immediately notify the sender If you have received this e-mail in error and delete it from your computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kdavoli <@t> gmail.com Tue May 28 11:05:41 2013 From: kdavoli <@t> gmail.com (Kate Davoli) Date: Tue May 28 11:06:12 2013 Subject: [Histonet] Leica microtome, buying used vs. new? Message-ID: Hi All, I need to acquire a Leica RM2235 microtome. I've got a quote from Leica directly, and several quotes from refurbished / used ones too with warranties for 6 months parts and labor. So here's my question: *does anyone recommend strongly against getting refurbished models?* The list seems to appreciate Leica products in general, partly because of how reliable the machinery is. So are there serious drawbacks to buying second hand? Are there certain refurbishing vendors that are more reliable than others? Any advice would be greatly appreciated. Thanks in advance, Katherine Davoli Supervisor, Tissue Culture & Histology Core Department of Ophthalmology University of Pittsburgh Mail Stop Code: EEI010901 928 Eye & Ear Institute 203 Lothrop Street Pittsburgh, PA 15213 (412) 647-8256 From BMolinari <@t> texasheart.org Tue May 28 11:24:00 2013 From: BMolinari <@t> texasheart.org (Molinari, Betsy) Date: Tue May 28 11:24:11 2013 Subject: [Histonet] RE: another Movats question (paraffin) In-Reply-To: <756DBA97C5CB8A409AC032D519094BEE59C2F5C1@exchange-mbx.unipathllc.corp> References: <756DBA97C5CB8A409AC032D519094BEE59C2F5C1@exchange-mbx.unipathllc.corp> Message-ID: Donella, I use Verhoffs. ----------------------------- Betsy Molinari Senior Histology Research Technician 832-355-6524 | BMolinari@texasheart.org | www.texasheart.org 6770 Bertner Ave., MC 1-283, Houston, TX 77030 Confidentiality Notice: This message may be confidential and/or privileged. If you are not the intended recipient you may not review, disseminate or copy this e-mail, its contents and/or any attachments. Please immediately notify the sender If you have received this e-mail in error and delete it from your computer system. ----------------------------- -----Original Message----- From: Donella Stillings [mailto:DStillings@unipathdx.com] Sent: Tuesday, May 28, 2013 10:56 AM To: Molinari, Betsy Subject: RE: another Movats question (paraffin) Are you using Weigert's Hematoxylin or regular Hematoxylin? Donella -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Molinari, Betsy Sent: Tuesday, May 28, 2013 9:47 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] another Movats question (paraffin) Hi, The Alcian Blue in my Movats is very faint or seems to completely disappear. I have been running Movats for years and this is a new problem. I have checked lot #?s everything is the same. The solution is 1% pH 2.7. The slides are in solution for 20 mins, rinse for 5 mins in tap H2O, then in alkaline alcohol (90 ml 95% 10 ml ammonium hydroxide) for 1 hr then rinsed for 10 mins. But after hematoxylin the blue is either gone or very faint. Any suggestions? Thanks, Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston , TX 77030 832-355-6524 (lab) 832-355-6812 (fax) Betsy Molinari Senior Histology Research Technician 832-355-6524 | BMolinari@texasheart.org | www.texasheart.org 6770 Bertner Ave., MC 1-283, Houston, TX 77030 [Texas Heart Institute][THI News] [THI on Facebook] [THI on Flicker] [THI on Google] [THI on Pinterest] [THI on Twitter] [THI on You Tube] Confidentiality Notice: This message may be confidential and/or privileged. If you are not the intended recipient you may not review, disseminate or copy this e-mail, its contents and/or any attachments. Please immediately notify the sender If you have received this e-mail in error and delete it from your computer system. From dmccaig <@t> ckha.on.ca Tue May 28 11:24:24 2013 From: dmccaig <@t> ckha.on.ca (Diana McCaig) Date: Tue May 28 11:24:43 2013 Subject: [Histonet] Reagent containers for VIP5 Message-ID: Does anyone have spare reagent containers for a VIP5 they are willing to sell. I am looking for 3 to use exclusively for warm water wash. We are located in Ontario, Canada Diana From letbeachside <@t> hotmail.com Tue May 28 11:28:31 2013 From: letbeachside <@t> hotmail.com (Luis Teran) Date: Tue May 28 11:28:37 2013 Subject: [Histonet] Checklist Message-ID: Does anyone have a checklist for Thermo Shannon Cryotome? Sent from my iPhone From craigak12 <@t> gmail.com Tue May 28 13:37:30 2013 From: craigak12 <@t> gmail.com (Jb) Date: Tue May 28 13:37:37 2013 Subject: [Histonet] Temperature strips: Message-ID: <52D2C6C2-1A57-481A-AA6E-BFB2B63E2B76@gmail.com> Does anyone know if we have to keep our temperature strips from our decloaking chamber/pressure cooker for CAP? We have currently been stapling them to our log. Sent from my iPhone From Lynn.Burton <@t> Illinois.gov Tue May 28 14:01:25 2013 From: Lynn.Burton <@t> Illinois.gov (Burton, Lynn) Date: Tue May 28 14:01:31 2013 Subject: [Histonet] Temperature strips: In-Reply-To: <52D2C6C2-1A57-481A-AA6E-BFB2B63E2B76@gmail.com> References: <52D2C6C2-1A57-481A-AA6E-BFB2B63E2B76@gmail.com> Message-ID: That's what we do. Lynn Burton Animal Disease Lab Galesburg, Il -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jb Sent: Tuesday, May 28, 2013 1:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Temperature strips: Does anyone know if we have to keep our temperature strips from our decloaking chamber/pressure cooker for CAP? We have currently been stapling them to our log. Sent from my iPhone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From c.tague <@t> Pathologyarts.com Tue May 28 16:08:56 2013 From: c.tague <@t> Pathologyarts.com (Curt) Date: Tue May 28 16:09:01 2013 Subject: [Histonet] looking for an IHC tech Message-ID: <9C8F910F72893643B3C3793C3D67132B013816D5@PATHOLOGYSERVER.pathologyarts.local> We're a southern CA lab looking for someone to help grow our IHC dept. as well as contribute with some routine bench work. I'm not going to go into great detail here, please contact if you're interested in exploring the opportunity. PLEASE NO HEAD HUNTERS OR JOB PLACEMENT AGENTS. Curt From krista.sider <@t> utoronto.ca Tue May 28 17:23:09 2013 From: krista.sider <@t> utoronto.ca (Krista Sider) Date: Tue May 28 17:23:14 2013 Subject: AW: [Histonet] Movat Pentachrome - Need to enhance Alcian Blue In-Reply-To: <001d01ce5bac$ce5c48a0$6b14d9e0$@gmx.at> References: <001d01ce5bac$ce5c48a0$6b14d9e0$@gmx.at> Message-ID: Hi Gudrun How deep would you consider the oxidation to go? I have tried samples from around 200um into the block. The Alkaline Alcohol converts the alcian blue to monastral fast blue, which is insoluble - this is what i have been told. So locks it in I guess. Doesn't really matter as I don't get alcian blue in the sample to lock in. Thanks Krista On May 28, 2013 10:09 AM, "Gudrun Lang" wrote: > Hi Krista, > Maybe the bad stainability is more due to oxidation by air of the old > block-surface than due to fixation. A deeper slide for comparison would > show. > I don't know what the alkaline alcohol is good for ? > > Gudrun > > -----Urspr?ngliche Nachricht----- > Von: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Krista > Sider > Gesendet: Montag, 27. Mai 2013 23:28 > An: histonet@lists.utsouthwestern.edu > Betreff: [Histonet] Movat Pentachrome - Need to enhance Alcian Blue > > Hi All, > > I had wonderful help from this site for my Woodstain Scarlet-Acid Fuchsin > binding collagen problem. Now that this is fixed I have realized that the > Alcian Blue is not staining my excessively fixed samples (Human Aorta & > Aortic Valve, formalin fixed 2-4 years, embedded in paraffin and stored at > RT for ~6 years). I have some samples fixed for only 2-6 months and these > are fine but the samples fixed for 2-4 years do not uptake the Alcian Blue. > I have taken it up to 1 hr in the 1% Alcian Blue solution followed by 10 > min > in 56*C Alkaline Alcohol solution. I do a 1hr Bouin's 50*C to start (I have > also tried Bouin's at 60*C). > > Any ideas on how I can get the Alcian Blue to stain these excessively fixed > samples or possibly a substitute? I need to see the proteoglycans/GAGs. > > Thank you > Krista > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From jordan_2585 <@t> yahoo.com Tue May 28 18:56:27 2013 From: jordan_2585 <@t> yahoo.com (Jordan Phillips) Date: Tue May 28 18:56:11 2013 Subject: [Histonet] Bone Marrow Issues Message-ID: HI everyone. We are currently having problems with all of our bone marrows, mainly clots, washing off the slides. We fix our bone marrows in Zenkers. We use positive charged slides, put the slides in the oven for a minimum of 2 hours and hand depar with no agitation. When we get to the water, the sections just float off the slides, leaving just a rim of the tissue. Any tips or suggestions would be greatly appreciated! From GAshton <@t> picr.man.ac.uk Wed May 29 03:57:21 2013 From: GAshton <@t> picr.man.ac.uk (Garry Ashton) Date: Wed May 29 03:54:15 2013 Subject: [Histonet] mouse Ki67 & P53 Message-ID: Hi all, Dako have recently stopped supplying their rat anti mouse Ki67. I'm also looking for a good anti mouse P53 antibody. Can anybody recommend any antibodies for either of these. Many thanks in advance Garry ________________________________ This email is confidential and intended solely for the use of the person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author and do not necessarily represent those of the Paterson Institute for Cancer Research or the University of Manchester. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. From BMolinari <@t> texasheart.org Wed May 29 06:45:21 2013 From: BMolinari <@t> texasheart.org (Molinari, Betsy) Date: Wed May 29 06:45:31 2013 Subject: [Histonet] another Movats question (paraffin) In-Reply-To: <2854F2313D8249AA897EAEB6D0F43ACF@HP2010> References: <2854F2313D8249AA897EAEB6D0F43ACF@HP2010> Message-ID: Hi Peggy, No, this is a batch I have been using since 12/12. I purchased it from Sigma. It seems faint after the ammonium hydroxide . I did a run yesterday and I did get some blue, but faintly, not as bright as I am used to seeing. Everything else was picture perfect. Ray suggested that it may be a change in my tap water. I have our current report and am waiting on the report before that one. I am doing another run today and am considering rinsing in DH2O after the Alcian Blue and ammonium hydroxide. What do you think? Betsy ----------------------------- Betsy Molinari Senior Histology Research Technician 832-355-6524 | BMolinari@texasheart.org | www.texasheart.org 6770 Bertner Ave., MC 1-283, Houston, TX 77030 Confidentiality Notice: This message may be confidential and/or privileged. If you are not the intended recipient you may not review, disseminate or copy this e-mail, its contents and/or any attachments. Please immediately notify the sender If you have received this e-mail in error and delete it from your computer system. ----------------------------- -----Original Message----- From: Lee & Peggy Wenk [mailto:lpwenk@sbcglobal.net] Sent: Tuesday, May 28, 2013 6:40 PM To: Molinari, Betsy Subject: Re: [Histonet] another Movats question (paraffin) Any chance this is a new batch of alcian blue? From where did you purchase it? I have a theory, but would like some more information first. Peggy A. Wenk, HTL(ASCP)SLS Lee & Peggy Wenk -----Original Message----- From: Molinari, Betsy Sent: Tuesday, May 28, 2013 11:47 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] another Movats question (paraffin) Hi, The Alcian Blue in my Movats is very faint or seems to completely disappear. I have been running Movats for years and this is a new problem. I have checked lot #?s everything is the same. The solution is 1% pH 2.7. The slides are in solution for 20 mins, rinse for 5 mins in tap H2O, then in alkaline alcohol (90 ml 95% 10 ml ammonium hydroxide) for 1 hr then rinsed for 10 mins. But after hematoxylin the blue is either gone or very faint. Any suggestions? Thanks, Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston , TX 77030 832-355-6524 (lab) 832-355-6812 (fax) Betsy Molinari Senior Histology Research Technician 832-355-6524 | BMolinari@texasheart.org | www.texasheart.org 6770 Bertner Ave., MC 1-283, Houston, TX 77030 [Texas Heart Institute][THI News] [THI on Facebook] [THI on Flicker] [THI on Google] [THI on Pinterest] [THI on Twitter] [THI on You Tube] Confidentiality Notice: This message may be confidential and/or privileged. If you are not the intended recipient you may not review, disseminate or copy this e-mail, its contents and/or any attachments. Please immediately notify the sender If you have received this e-mail in error and delete it from your computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From becky.garrison <@t> jax.ufl.edu Wed May 29 09:56:10 2013 From: becky.garrison <@t> jax.ufl.edu (Garrison, Becky) Date: Wed May 29 09:56:17 2013 Subject: [Histonet] Tracking OR specimens In-Reply-To: References: Message-ID: <9E47DE9D490DCC42A2EAE94F22BF93F240B9D2DD@JX1B-MAIL1.umc.ufl.edu> Realize this is a late response.... I can pull a "Specimen report" through Centricity (the OR computer system). The OR staff enters each specimen as it is collected (for pathology, cultures for micro, frozens, touch preps) into Centricity as part of their documentation for the case. The report I pull extracts this data. I run each day after 12 noon for the previous day. We only instituted this in April. Gives great feedback and lets me check that the specimen is received in Pathology but that the pathology accession staff accurately records each specimen. We also get the OR schedule but not all cases on schedule generate a specimen and some cases expected to have pathology specimen don't and other cases not expected to generate a specimen do ...... so the Specimen Report is the most specific way to track what was intended to go to Pathology. Unfortunately, we are converting hospital wide to EPIC and so far, EPIC OR documentation does not have a comparable function. Orders will then be placed not in an OR module but in the same place that all other orders are placed and our Order Interface does not give specific specimen sites. If there are EPIC users / EPIC vendors that have a solution, I would really like to hear from you. If you use Centricity and want more specifics, will be glad to give more information on our set up. Becky Garrison Pathology Supervisor Shands Jacksonville Jacksonville, FL 32209 904-244-6237, phone 904-244-4290, fax 904-393-3194, pager -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO Sent: Monday, March 18, 2013 11:25 AM To: Michelle Lamphere; histonet Subject: RE: [Histonet] Tracking OR specimens We use the OR schedule and have a log at Surgical Pathology that the person delivering specimens places a patient label w/ the number of specimens delivered and they sign off. This has helped many times when OR believes they have delivered all the specimens. We use both the log and OR schedule to reconcile cases and contact OR is a case has not been delivered by end of day. William DeSalvo, BS HTL(ASCP) Production Manager-Anatomic Pathology Chair, NSH Quality Management Committee Owner/Consultant, Collaborative Advantage Consulting > From: MICHELLE.LAMPHERE@childrens.com > To: histonet@lists.utsouthwestern.edu > Date: Mon, 18 Mar 2013 13:14:04 +0000 > Subject: [Histonet] Tracking OR specimens > > Are there any histology labs that actively participate in auditing the Operating Room on a daily basis to make sure that histology receives all of the specimens that the OR should have submitted? If so, how do you do this? Or should the OR be solely responsible for making sure that they specimens make it to histology? > > Michelle M Lamphere, HT (ASCP) > Senior Tech, Histology > Children's Medical Center > 1935 Medical District Drive > Dallas, TX 75235 > Office :214-456-2798 > Histology: 214-456-2318 > Fax: 214-456-0779 > > > Please consider the environment before printing this e-mail > > This e-mail, facsimile, or letter and any files or attachments transmitted with it contains > information that is confidential and privileged. This information is intended only for the use of the > individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further > disclosures are prohibited without proper authorization. If you are not the intended recipient, any > disclosure, copying, printing, or use of this information is strictly prohibited and possibly a > violation of federal or state law and regulations. If you have received this information in error, > please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at > privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all > applicable privileges related to this information. > > Please consider the environment before printing this e-mail. > > This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MICHELLE.LAMPHERE <@t> childrens.com Wed May 29 10:20:13 2013 From: MICHELLE.LAMPHERE <@t> childrens.com (Michelle Lamphere) Date: Wed May 29 10:20:20 2013 Subject: [Histonet] Tracking OR specimens In-Reply-To: <9E47DE9D490DCC42A2EAE94F22BF93F240B9D2DD@JX1B-MAIL1.umc.ufl.edu> References: <9E47DE9D490DCC42A2EAE94F22BF93F240B9D2DD@JX1B-MAIL1.umc.ufl.edu> Message-ID: We currently have EPIC as our hospital wide system and Cerner as the Lab system. It is a nightmare for specimen tracking, meaning there is not simple way to do it. That is why I was asking the question. Our OR wanted to put the burden of responsibility on the histo lab for making sure that they get all of their specimens to us. Just having them correct a problem with a specimen that they do submit can be an hours long or even days long process involving multiple conversations and I really wanted no part of having to keep track of their specimens and then finding them if there is something missing. I related it to asking them to come in and help us find a block or slide when we can't find it. Currently, EPIC interfaces with the general lab orders, but not AP (and Micro?) As far as I know, there is no tracking in EPIC. What we suggested was giving them access to print our case log out of Cerner and then they could go through it to make sure we had received everything. What we will eventually end up doing is have a real time running board that is an interface between EPIC and Cerner. Our main lab uses it successfully. Essentially, the OR will order AP specimens in EPIC like they would a gen lab order. That order will actually come across the interface as a gen lab order. When we receive the specimen in histology, that will indicate to Cerner that the case is "complete". We will have a monitor in the histology lab and one in the OR. As specimens are entered in EPIC, they will show up on the monitor in both the OR and histology. As we receive them, they will drop off the list. Both departments will then have a running log of every specimen from the OR. At least that is the theory..... Michelle -----Original Message----- From: Garrison, Becky [mailto:becky.garrison@jax.ufl.edu] Sent: Wednesday, May 29, 2013 9:56 AM To: 'WILLIAM DESALVO'; Michelle Lamphere; histonet Subject: RE: [Histonet] Tracking OR specimens Realize this is a late response.... I can pull a "Specimen report" through Centricity (the OR computer system). The OR staff enters each specimen as it is collected (for pathology, cultures for micro, frozens, touch preps) into Centricity as part of their documentation for the case. The report I pull extracts this data. I run each day after 12 noon for the previous day. We only instituted this in April. Gives great feedback and lets me check that the specimen is received in Pathology but that the pathology accession staff accurately records each specimen. We also get the OR schedule but not all cases on schedule generate a specimen and some cases expected to have pathology specimen don't and other cases not expected to generate a specimen do ...... so the Specimen Report is the most specific way to track what was intended to go to Pathology. Unfortunately, we are converting hospital wide to EPIC and so far, EPIC OR documentation does not have a comparable function. Orders will then be placed not in an OR module but in the same place that all other orders are placed and our Order Interface does not give specific specimen sites. If there are EPIC users / EPIC vendors that have a solution, I would really like to hear from you. If you use Centricity and want more specifics, will be glad to give more information on our set up. Becky Garrison Pathology Supervisor Shands Jacksonville Jacksonville, FL 32209 904-244-6237, phone 904-244-4290, fax 904-393-3194, pager -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO Sent: Monday, March 18, 2013 11:25 AM To: Michelle Lamphere; histonet Subject: RE: [Histonet] Tracking OR specimens We use the OR schedule and have a log at Surgical Pathology that the person delivering specimens places a patient label w/ the number of specimens delivered and they sign off. This has helped many times when OR believes they have delivered all the specimens. We use both the log and OR schedule to reconcile cases and contact OR is a case has not been delivered by end of day. William DeSalvo, BS HTL(ASCP) Production Manager-Anatomic Pathology Chair, NSH Quality Management Committee Owner/Consultant, Collaborative Advantage Consulting > From: MICHELLE.LAMPHERE@childrens.com > To: histonet@lists.utsouthwestern.edu > Date: Mon, 18 Mar 2013 13:14:04 +0000 > Subject: [Histonet] Tracking OR specimens > > Are there any histology labs that actively participate in auditing the Operating Room on a daily basis to make sure that histology receives all of the specimens that the OR should have submitted? If so, how do you do this? Or should the OR be solely responsible for making sure that they specimens make it to histology? > > Michelle M Lamphere, HT (ASCP) > Senior Tech, Histology > Children's Medical Center > 1935 Medical District Drive > Dallas, TX 75235 > Office :214-456-2798 > Histology: 214-456-2318 > Fax: 214-456-0779 > > > Please consider the environment before printing this e-mail > > This e-mail, facsimile, or letter and any files or attachments > transmitted with it contains information that is confidential and > privileged. This information is intended only for the use of the > individual(s) and entity(ies) to whom it is addressed. If you are the > intended recipient, further disclosures are prohibited without proper > authorization. If you are not the intended recipient, any disclosure, > copying, printing, or use of this information is strictly prohibited > and possibly a violation of federal or state law and regulations. If > you have received this information in error, please notify Children's > Medical Center Dallas immediately at 214-456-4444 or via e-mail at privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. > > Please consider the environment before printing this e-mail. > > This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Please consider the environment before printing this e-mail. This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. From POWELL_SA <@t> mercer.edu Wed May 29 10:31:00 2013 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Wed May 29 10:31:05 2013 Subject: [Histonet] Bone Marrow Issues In-Reply-To: References: Message-ID: <9BF995BC0E47744E9673A41486E24EE2579CBC3C84@MERCERMAIL.MercerU.local> I had this happen when I was doing IHC and changed to regular slides and used StayOn from Leica. Had more success with keeping the tissue on the slides. I now do autopsy slides and do the same. Shirley -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jordan Phillips Sent: Tuesday, May 28, 2013 7:56 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Bone Marrow Issues HI everyone. We are currently having problems with all of our bone marrows, mainly clots, washing off the slides. We fix our bone marrows in Zenkers. We use positive charged slides, put the slides in the oven for a minimum of 2 hours and hand depar with no agitation. When we get to the water, the sections just float off the slides, leaving just a rim of the tissue. Any tips or suggestions would be greatly appreciated! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From madeathridge <@t> pastnashville.com Wed May 29 10:48:04 2013 From: madeathridge <@t> pastnashville.com (Mary Ann Deathridge) Date: Wed May 29 10:48:47 2013 Subject: [Histonet] BRAF Message-ID: <3401db87$20d7835b$738d806f$@com> Hi Hisonetters Looking for information on BRAF-(V600E) IHC. Any information (vendor, procedure..etc) greatly appreciated. Thanks. From mkent <@t> dermpathlab.com Wed May 29 12:14:27 2013 From: mkent <@t> dermpathlab.com (Michael Kent) Date: Wed May 29 12:14:47 2013 Subject: [Histonet] BRAF In-Reply-To: <3401db87$20d7835b$738d806f$@com> References: <3401db87$20d7835b$738d806f$@com> Message-ID: <27EB7FFB1A15F549B17F79B1A856C70BCDB705@dlcs-sbs1.DPLCS.intra> There is an RUO BRAF v600 clone VE1 from Spring Biosciences cited in literature. I have not found an ASR or IVD. Confidentiality Notice: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. Thank you. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mary Ann Deathridge Sent: Wednesday, May 29, 2013 11:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] BRAF Hi Hisonetters Looking for information on BRAF-(V600E) IHC. Any information (vendor, procedure..etc) greatly appreciated. Thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mcauliff <@t> umdnj.edu Wed May 29 12:52:49 2013 From: mcauliff <@t> umdnj.edu (Geoff) Date: Wed May 29 12:52:33 2013 Subject: [Histonet] need manual for AO 860 sliding microtome In-Reply-To: <27EB7FFB1A15F549B17F79B1A856C70BCDB705@dlcs-sbs1.DPLCS.intra> References: <3401db87$20d7835b$738d806f$@com> <27EB7FFB1A15F549B17F79B1A856C70BCDB705@dlcs-sbs1.DPLCS.intra> Message-ID: <51A64071.9010407@umdnj.edu> Greetings Histoland: I have an old AO 860 sliding microtome that needs cleaning and lubrication, it skips sections. Anyone have a service manual, either paper or PDF, they can share? Advice would work too. Thanks in advance. Geoff -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From kgrobert <@t> rci.rutgers.edu Wed May 29 13:14:27 2013 From: kgrobert <@t> rci.rutgers.edu (kgrobert@rci.rutgers.edu) Date: Wed May 29 13:14:32 2013 Subject: [Histonet] Processing thin slices of mouse cerebellum Message-ID: <1fd19caf86a3f5b9bb14e12677813a2b.squirrel@webmail.rci.rutgers.edu> To all, We have these very thin slices of mouse cerebellum that need to be processed into paraffin. The problem is that they need to be kept flat. We have tried sponges, but some of the slices are so small that they shrink (processed with our biopsy program, of course; if you want details, let me know) and get lost in the holes of the sponges. We have biopsy cassettes, but the slices will still tumble and not stay flat. We have processed other things in lens paper, but I had to scrape the tissue with the paraffin into the mold. I have not tried Histogel, though-maybe embed the slices flat in that first and then process it? Is there anything else? Thanks so much! Kathleen Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology & Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (848) 445-1443 FAX (732) 445-6905 From lpwenk <@t> sbcglobal.net Wed May 29 15:18:02 2013 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Wed May 29 15:18:09 2013 Subject: [Histonet] another Movats question (paraffin) In-Reply-To: References: <2854F2313D8249AA897EAEB6D0F43ACF@HP2010> Message-ID: What happens if you skip the ammonium hydroxide? Peggy Wenk -----Original Message----- From: Molinari, Betsy Sent: Wednesday, May 29, 2013 7:45 AM To: Lee & Peggy Wenk ; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] another Movats question (paraffin) Hi Peggy, No, this is a batch I have been using since 12/12. I purchased it from Sigma. It seems faint after the ammonium hydroxide . I did a run yesterday and I did get some blue, but faintly, not as bright as I am used to seeing. Everything else was picture perfect. Ray suggested that it may be a change in my tap water. I have our current report and am waiting on the report before that one. I am doing another run today and am considering rinsing in DH2O after the Alcian Blue and ammonium hydroxide. What do you think? Betsy ----------------------------- Betsy Molinari Senior Histology Research Technician 832-355-6524 | BMolinari@texasheart.org | www.texasheart.org 6770 Bertner Ave., MC 1-283, Houston, TX 77030 Confidentiality Notice: This message may be confidential and/or privileged. If you are not the intended recipient you may not review, disseminate or copy this e-mail, its contents and/or any attachments. Please immediately notify the sender If you have received this e-mail in error and delete it from your computer system. ----------------------------- -----Original Message----- From: Lee & Peggy Wenk [mailto:lpwenk@sbcglobal.net] Sent: Tuesday, May 28, 2013 6:40 PM To: Molinari, Betsy Subject: Re: [Histonet] another Movats question (paraffin) Any chance this is a new batch of alcian blue? From where did you purchase it? I have a theory, but would like some more information first. Peggy A. Wenk, HTL(ASCP)SLS Lee & Peggy Wenk -----Original Message----- From: Molinari, Betsy Sent: Tuesday, May 28, 2013 11:47 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] another Movats question (paraffin) Hi, The Alcian Blue in my Movats is very faint or seems to completely disappear. I have been running Movats for years and this is a new problem. I have checked lot #?s everything is the same. The solution is 1% pH 2.7. The slides are in solution for 20 mins, rinse for 5 mins in tap H2O, then in alkaline alcohol (90 ml 95% 10 ml ammonium hydroxide) for 1 hr then rinsed for 10 mins. But after hematoxylin the blue is either gone or very faint. Any suggestions? Thanks, Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston , TX 77030 832-355-6524 (lab) 832-355-6812 (fax) Betsy Molinari Senior Histology Research Technician 832-355-6524 | BMolinari@texasheart.org | www.texasheart.org 6770 Bertner Ave., MC 1-283, Houston, TX 77030 [Texas Heart Institute][THI News] [THI on Facebook] [THI on Flicker] [THI on Google] [THI on Pinterest] [THI on Twitter] [THI on You Tube] Confidentiality Notice: This message may be confidential and/or privileged. If you are not the intended recipient you may not review, disseminate or copy this e-mail, its contents and/or any attachments. Please immediately notify the sender If you have received this e-mail in error and delete it from your computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sdysart <@t> mirnarx.com Wed May 29 15:20:10 2013 From: sdysart <@t> mirnarx.com (Sarah Dysart) Date: Wed May 29 15:20:18 2013 Subject: [Histonet] Extra Money Message-ID: Anyone know any ideas on how to make a couple extra bucks a month using our histology-awesomeness?? Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 From joelleweaver <@t> hotmail.com Wed May 29 15:31:11 2013 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Wed May 29 15:31:16 2013 Subject: [Histonet] Extra Money In-Reply-To: References: Message-ID: Publish Joelle Weaver MAOM, HTL (ASCP) QIHC > From: sdysart@mirnarx.com > To: histonet@lists.utsouthwestern.edu > Date: Wed, 29 May 2013 20:20:10 +0000 > Subject: [Histonet] Extra Money > > Anyone know any ideas on how to make a couple extra bucks a month using our histology-awesomeness?? > > > Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) > Histotechnologist > Mirna Therapeutics > 2150 Woodward Street > Suite 100 > Austin, Texas 78744 > (512)901-0900 ext. 6912 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed May 29 15:36:41 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed May 29 15:36:45 2013 Subject: [Histonet] Extra Money In-Reply-To: References: Message-ID: <1369859801.99883.YahooMailNeo@web163103.mail.bf1.yahoo.com> Not even that! If you publish you will build a better c.v. but that is not assurance tof being appreciated by any prospective employer. In the rare event that it is appreciated you will be able to negotiate a better salary, but that is all! Ren? J. From: joelle weaver To: Sarah Dysart ; "histonet@lists.utsouthwestern.edu" Sent: Wednesday, May 29, 2013 4:31 PM Subject: RE: [Histonet] Extra Money Publish? Joelle Weaver MAOM, HTL (ASCP) QIHC > From: sdysart@mirnarx.com > To: histonet@lists.utsouthwestern.edu > Date: Wed, 29 May 2013 20:20:10 +0000 > Subject: [Histonet] Extra Money > > Anyone know any ideas on how to make a couple extra bucks a month using our histology-awesomeness?? > > > Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) > Histotechnologist > Mirna Therapeutics > 2150 Woodward Street > Suite 100 > Austin, Texas? 78744 > (512)901-0900 ext. 6912 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ??? ??? ??? ? ??? ??? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sdysart <@t> mirnarx.com Wed May 29 16:05:02 2013 From: sdysart <@t> mirnarx.com (Sarah Dysart) Date: Wed May 29 16:05:12 2013 Subject: [Histonet] Extra Money In-Reply-To: <1369859801.99883.YahooMailNeo@web163103.mail.bf1.yahoo.com> References: <1369859801.99883.YahooMailNeo@web163103.mail.bf1.yahoo.com> Message-ID: <3a6b3dd518c0423ca60e7f40a74c8f1c@BLUPR07MB001.namprd07.prod.outlook.com> I plan on doing a poster for NSH...does that count =) Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: Wednesday, May 29, 2013 3:37 PM To: joelle weaver; Sarah Dysart; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Extra Money Not even that! If you publish you will build a better c.v. but that is not assurance tof being appreciated by any prospective employer. In the rare event that it is appreciated you will be able to negotiate a better salary, but that is all! Ren? J. From: joelle weaver To: Sarah Dysart ; "histonet@lists.utsouthwestern.edu" Sent: Wednesday, May 29, 2013 4:31 PM Subject: RE: [Histonet] Extra Money Publish Joelle Weaver MAOM, HTL (ASCP) QIHC > From: sdysart@mirnarx.com > To: histonet@lists.utsouthwestern.edu > Date: Wed, 29 May 2013 20:20:10 +0000 > Subject: [Histonet] Extra Money > > Anyone know any ideas on how to make a couple extra bucks a month using our histology-awesomeness?? > > > Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) > Histotechnologist > Mirna Therapeutics > 2150 Woodward Street > Suite 100 > Austin, Texas 78744 > (512)901-0900 ext. 6912 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpwenk <@t> sbcglobal.net Wed May 29 17:09:50 2013 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Wed May 29 17:09:57 2013 Subject: [Histonet] Extra Money In-Reply-To: <3a6b3dd518c0423ca60e7f40a74c8f1c@BLUPR07MB001.namprd07.prod.outlook.com> References: <1369859801.99883.YahooMailNeo@web163103.mail.bf1.yahoo.com> <3a6b3dd518c0423ca60e7f40a74c8f1c@BLUPR07MB001.namprd07.prod.outlook.com> Message-ID: <54B88D78A05E4335B5DB94F3CB3EB19E@HP2010> I don't suppose you want to hear this, but several people are working extra jobs. Lee Wenk (not Peggy). -----Original Message----- From: Sarah Dysart Sent: Wednesday, May 29, 2013 5:05 PM To: Rene J Buesa ; joelle weaver ; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Extra Money I plan on doing a poster for NSH...does that count =) Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: Wednesday, May 29, 2013 3:37 PM To: joelle weaver; Sarah Dysart; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Extra Money Not even that! If you publish you will build a better c.v. but that is not assurance tof being appreciated by any prospective employer. In the rare event that it is appreciated you will be able to negotiate a better salary, but that is all! Ren? J. From: joelle weaver To: Sarah Dysart ; "histonet@lists.utsouthwestern.edu" Sent: Wednesday, May 29, 2013 4:31 PM Subject: RE: [Histonet] Extra Money Publish Joelle Weaver MAOM, HTL (ASCP) QIHC > From: sdysart@mirnarx.com > To: > histonet@lists.utsouthwestern.edu > Date: Wed, 29 May 2013 20:20:10 +0000 > Subject: [Histonet] Extra Money > > Anyone know any ideas on how to make a couple extra bucks a month using > our histology-awesomeness?? > > > Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) > Histotechnologist > Mirna Therapeutics > 2150 Woodward Street > Suite 100 > Austin, Texas 78744 > (512)901-0900 ext. 6912 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From latecor <@t> montevideo.com.uy Wed May 29 22:41:13 2013 From: latecor <@t> montevideo.com.uy (C.D.G.) Date: Wed May 29 22:41:28 2013 Subject: [Histonet] Bone Marrow Issues In-Reply-To: <9BF995BC0E47744E9673A41486E24EE2579CBC3C84@MERCERMAIL.MercerU.local> References: <9BF995BC0E47744E9673A41486E24EE2579CBC3C84@MERCERMAIL.MercerU.local> Message-ID: <201305300041130856.001B01C1@smtp.montevideo.com.uy> Shiley: positive charged slides do not mantain certain tissues on them well. Blood is one of them, and it is advisable to mount your bloody bone marrows on slides treated with vinyl glue, like Elmer's or the one you prefer. My regards, Carlos. *********** REPLY SEPARATOR *********** On 29/05/2013 at 11:31 a.m. Shirley A. Powell wrote: >I had this happen when I was doing IHC and changed to regular slides and >used StayOn from Leica. Had more success with keeping the tissue on the >slides. I now do autopsy slides and do the same. > >Shirley > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jordan >Phillips >Sent: Tuesday, May 28, 2013 7:56 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Bone Marrow Issues > >HI everyone. We are currently having problems with all of our bone >marrows, mainly clots, washing off the slides. We fix our bone marrows in >Zenkers. We use positive charged slides, put the slides in the oven for a >minimum of 2 hours and hand depar with no agitation. When we get to the >water, the sections just float off the slides, leaving just a rim of the >tissue. Any tips or suggestions would be greatly appreciated! >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Thu May 30 08:10:46 2013 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Thu May 30 08:10:55 2013 Subject: AW: [Histonet] Processing thin slices of mouse cerebellum In-Reply-To: <1fd19caf86a3f5b9bb14e12677813a2b.squirrel@webmail.rci.rutgers.edu> References: <1fd19caf86a3f5b9bb14e12677813a2b.squirrel@webmail.rci.rutgers.edu> Message-ID: <000601ce5d37$1aa18320$4fe48960$@gmx.at> Is the tissue fixed well enough? At least two days? Processing through graded ethanols with little difference should minimize shrinkage. Instead of 70-96-100, take 50-60-70-80-96-100. Times shouldn't be too short. Fatty brain needs more time for reagens-exchange than other small biopsies. ...smooth protocol. For embedding one can try to press slightly the tissue with a warm small metalblock on the ground of the mold Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von kgrobert@rci.rutgers.edu Gesendet: Mittwoch, 29. Mai 2013 20:14 An: histonet Betreff: [Histonet] Processing thin slices of mouse cerebellum To all, We have these very thin slices of mouse cerebellum that need to be processed into paraffin. The problem is that they need to be kept flat. We have tried sponges, but some of the slices are so small that they shrink (processed with our biopsy program, of course; if you want details, let me know) and get lost in the holes of the sponges. We have biopsy cassettes, but the slices will still tumble and not stay flat. We have processed other things in lens paper, but I had to scrape the tissue with the paraffin into the mold. I have not tried Histogel, though-maybe embed the slices flat in that first and then process it? Is there anything else? Thanks so much! Kathleen Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology & Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (848) 445-1443 FAX (732) 445-6905 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sdysart <@t> mirnarx.com Thu May 30 08:26:11 2013 From: sdysart <@t> mirnarx.com (Sarah Dysart) Date: Thu May 30 08:26:18 2013 Subject: [Histonet] Extra Money In-Reply-To: <54B88D78A05E4335B5DB94F3CB3EB19E@HP2010> References: <1369859801.99883.YahooMailNeo@web163103.mail.bf1.yahoo.com> <3a6b3dd518c0423ca60e7f40a74c8f1c@BLUPR07MB001.namprd07.prod.outlook.com> <54B88D78A05E4335B5DB94F3CB3EB19E@HP2010> Message-ID: I already PRN at a local hospital, but that is usually only one or two shifts a month...I need something to add a third job to bring in extra dollars. I hope you weren't trying to act like I was being lazy or complaining Lee...as I am not either one...I have a new baby and the strain of putting her into daycare is A LOT!! Just trying to stay afloat. Thank you for everyone's legitimate suggestions. I will try a few of those. Thanks again! Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk Sent: Wednesday, May 29, 2013 5:10 PM To: Histonet Subject: Re: [Histonet] Extra Money I don't suppose you want to hear this, but several people are working extra jobs. Lee Wenk (not Peggy). -----Original Message----- From: Sarah Dysart Sent: Wednesday, May 29, 2013 5:05 PM To: Rene J Buesa ; joelle weaver ; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Extra Money I plan on doing a poster for NSH...does that count =) Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: Wednesday, May 29, 2013 3:37 PM To: joelle weaver; Sarah Dysart; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Extra Money Not even that! If you publish you will build a better c.v. but that is not assurance tof being appreciated by any prospective employer. In the rare event that it is appreciated you will be able to negotiate a better salary, but that is all! Ren? J. From: joelle weaver To: Sarah Dysart ; "histonet@lists.utsouthwestern.edu" Sent: Wednesday, May 29, 2013 4:31 PM Subject: RE: [Histonet] Extra Money Publish Joelle Weaver MAOM, HTL (ASCP) QIHC > From: sdysart@mirnarx.com > To: > histonet@lists.utsouthwestern.edu > Date: Wed, 29 May 2013 20:20:10 +0000 > Subject: [Histonet] Extra Money > > Anyone know any ideas on how to make a couple extra bucks a month using > our histology-awesomeness?? > > > Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) > Histotechnologist > Mirna Therapeutics > 2150 Woodward Street > Suite 100 > Austin, Texas 78744 > (512)901-0900 ext. 6912 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu May 30 08:45:55 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu May 30 08:45:59 2013 Subject: [Histonet] Extra Money In-Reply-To: <3a6b3dd518c0423ca60e7f40a74c8f1c@BLUPR07MB001.namprd07.prod.outlook.com> References: <1369859801.99883.YahooMailNeo@web163103.mail.bf1.yahoo.com> <3a6b3dd518c0423ca60e7f40a74c8f1c@BLUPR07MB001.namprd07.prod.outlook.com> Message-ID: <1369921555.51735.YahooMailNeo@web163103.mail.bf1.yahoo.com> Yes, but?only if?you incorporate that activity to your c.v. Ren? J. From: Sarah Dysart To: Rene J Buesa ; joelle weaver ; "histonet@lists.utsouthwestern.edu" Sent: Wednesday, May 29, 2013 5:05 PM Subject: RE: [Histonet] Extra Money I plan on doing a poster for NSH?does that count =) ? Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas? 78744 (512)901-0900 ext. 6912 ? From:Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: Wednesday, May 29, 2013 3:37 PM To: joelle weaver; Sarah Dysart; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Extra Money ? Not even that! If you publish you will build a better c.v. but that is not assurance tof being appreciated by any prospective employer. In the rare event that it is appreciated you will be able to negotiate a better salary, but that is all! Ren?J. ? From:joelle weaver To: Sarah Dysart ; "histonet@lists.utsouthwestern.edu" Sent: Wednesday, May 29, 2013 4:31 PM Subject: RE: [Histonet] Extra Money Publish? Joelle Weaver MAOM, HTL (ASCP) QIHC > From: sdysart@mirnarx.com > To: histonet@lists.utsouthwestern.edu > Date: Wed, 29 May 2013 20:20:10 +0000 > Subject: [Histonet] Extra Money > > Anyone know any ideas on how to make a couple extra bucks a month using our histology-awesomeness?? > > > Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) > Histotechnologist > Mirna Therapeutics > 2150 Woodward Street > Suite 100 > Austin, Texas? 78744 > (512)901-0900 ext. 6912 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ??? ??? ??? ? ??? ??? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu May 30 08:55:40 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu May 30 08:55:45 2013 Subject: [Histonet] Extra Money In-Reply-To: References: <1369859801.99883.YahooMailNeo@web163103.mail.bf1.yahoo.com> <3a6b3dd518c0423ca60e7f40a74c8f1c@BLUPR07MB001.namprd07.prod.outlook.com> <54B88D78A05E4335B5DB94F3CB3EB19E@HP2010> Message-ID: <1369922140.33070.YahooMailNeo@web163105.mail.bf1.yahoo.com> Another "solution" is to do the following: 1- honestly analyze your job performance and qualifications 2- objectively analyze how much your supervisor depends on your work and if with some frequency you "take care of business" while other colleagues do not. 3- IF both analysis "come favorable" to you, try to find out how much other histology labs are paying to colleagues with similar qualifications and work load to yours 4- If the result is that you are payed less, write down all you know, all you do, your productivity and how much you are paid. Ask for a meeting with your supervisor and?request a hourly or salary?raise. This could be another way of getting a bit more for what you know and do Ren? J. From: Sarah Dysart To: Lee & Peggy Wenk ; Histonet Sent: Thursday, May 30, 2013 9:26 AM Subject: RE: [Histonet] Extra Money I already PRN at a local hospital, but that is usually only one or two shifts a month...I need something to add a third job to bring in extra dollars.? I hope you weren't trying to act like I was being lazy or complaining Lee...as I am not either one...I have a new baby and the strain of putting her into daycare is A LOT!!? Just trying to stay afloat. Thank you for everyone's legitimate suggestions.? I will try a few of those. Thanks again! Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas? 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk Sent: Wednesday, May 29, 2013 5:10 PM To: Histonet Subject: Re: [Histonet] Extra Money I don't suppose you want to hear this, but several people are working extra jobs. Lee Wenk (not Peggy). -----Original Message----- From: Sarah Dysart Sent: Wednesday, May 29, 2013 5:05 PM To: Rene J Buesa ; joelle weaver ; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Extra Money I plan on doing a poster for NSH...does that count =) Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas? 78744 (512)901-0900 ext. 6912 From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: Wednesday, May 29, 2013 3:37 PM To: joelle weaver; Sarah Dysart; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Extra Money Not even that! If you publish you will build a better c.v. but that is not assurance tof being appreciated by any prospective employer. In the rare event that it is appreciated you will be able to negotiate a better salary, but that is all! Ren? J. From: joelle weaver To: Sarah Dysart ; "histonet@lists.utsouthwestern.edu" Sent: Wednesday, May 29, 2013 4:31 PM Subject: RE: [Histonet] Extra Money Publish Joelle Weaver MAOM, HTL (ASCP) QIHC > From: sdysart@mirnarx.com > To: > histonet@lists.utsouthwestern.edu > Date: Wed, 29 May 2013 20:20:10 +0000 > Subject: [Histonet] Extra Money > > Anyone know any ideas on how to make a couple extra bucks a month using > our histology-awesomeness?? > > > Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) > Histotechnologist > Mirna Therapeutics > 2150 Woodward Street > Suite 100 > Austin, Texas? 78744 > (512)901-0900 ext. 6912 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ? ? ? ? ? ? ? ? ? ? ? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Lynn.Burton <@t> Illinois.gov Thu May 30 09:54:57 2013 From: Lynn.Burton <@t> Illinois.gov (Burton, Lynn) Date: Thu May 30 09:55:09 2013 Subject: [Histonet] antigen retrieval question Message-ID: If anyone out there is using a decloaker for antigen retrieval, when doing ihc staining, would you please contact me on my e-mail address? I have been struggling with a BVD test for months. It is not working consistently and I want to explore using this method along with our Benchmark XT stainer from Ventana. Thank you, Lynn Burton Galesburg Animal Disease Lab Galesburg, Il lynn.burton@illinois.gov From rob <@t> foliobio.com Thu May 30 10:05:50 2013 From: rob <@t> foliobio.com (Rob Day) Date: Thu May 30 10:05:56 2013 Subject: [Histonet] antigen retrieval question In-Reply-To: References: Message-ID: Is this with an older tissue sample? We don't do antigen retrieval, but we do have a proprietary technique for tissue restoration which can help with problematic IHC studies, especially in older FFPE tissue samples. See here for some examples: http://hematoxylin-eosin-tales.blogspot.com/2013/04/new-technique-for-improving-staining-in.html We could treat your sections for free if you like. Let me know if you are interested. Rob Day. On May 30, 2013, at 10:54 AM, "Burton, Lynn" wrote: > If anyone out there is using a decloaker for antigen retrieval, when doing ihc staining, would you please contact me on my e-mail address? I have been struggling with a BVD test for months. It is not working consistently and I want to explore using this method along with our Benchmark XT stainer from Ventana. > Thank you, > Lynn Burton > Galesburg Animal Disease Lab > Galesburg, Il > lynn.burton@illinois.gov > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From GaleL <@t> unionhospital.org Thu May 30 10:26:00 2013 From: GaleL <@t> unionhospital.org (Gale Limron) Date: Thu May 30 10:26:26 2013 Subject: [Histonet] amyloid stain Message-ID: Hello, It's been awhile since we ran an amyloid stain.............Today, using Richard Allan Chromaview kit, our staining tech noticed a lot of precipitant in the working Congo Red solution. Any thoughts or advice? Thanks, Gale Gale Limron, HT, CT (ASCP) Union Hospital Dover, Oh This e-mail is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential or otherwise protected from disclosure. Dissemination, distribution or copying of this e-mail or the information herein by anyone other than the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, is prohibited. If you received this message in error, please delete without copying and kindly e-mail a reply to inform us of the mistake in delivery. From joelleweaver <@t> hotmail.com Thu May 30 10:29:30 2013 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Thu May 30 10:29:34 2013 Subject: [Histonet] Extra Money In-Reply-To: <1369859801.99883.YahooMailNeo@web163103.mail.bf1.yahoo.com> References: , <1369859801.99883.YahooMailNeo@web163103.mail.bf1.yahoo.com> Message-ID: I wasn't talking about "appreciation" - I meant more like work for hire. It is not much, but can be squeezed in around other commitments. So true, not likely to do much dramatic with your C.V. ! Joelle Weaver MAOM, HTL (ASCP) QIHC Date: Wed, 29 May 2013 13:36:41 -0700 From: rjbuesa@yahoo.com Subject: Re: [Histonet] Extra Money To: joelleweaver@hotmail.com; sdysart@mirnarx.com; histonet@lists.utsouthwestern.edu Not even that! If you publish you will build a better c.v. but that is not assurance tof being appreciated by any prospective employer. In the rare event that it is appreciated you will be able to negotiate a better salary, but that is all! Ren? J. From: joelle weaver To: Sarah Dysart ; "histonet@lists.utsouthwestern.edu" Sent: Wednesday, May 29, 2013 4:31 PM Subject: RE: [Histonet] Extra Money Publish Joelle Weaver MAOM, HTL (ASCP) QIHC > From: sdysart@mirnarx.com > To: histonet@lists.utsouthwestern.edu > Date: Wed, 29 May 2013 20:20:10 +0000 > Subject: [Histonet] Extra Money > > Anyone know any ideas on how to make a couple extra bucks a month using our histology-awesomeness?? > > > Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) > Histotechnologist > Mirna Therapeutics > 2150 Woodward Street > Suite 100 > Austin, Texas 78744 > (512)901-0900 ext. 6912 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu May 30 10:32:24 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu May 30 10:32:31 2013 Subject: [Histonet] amyloid stain In-Reply-To: References: Message-ID: <1369927944.92158.YahooMailNeo@web163101.mail.bf1.yahoo.com> Check/change the solution Ren? J. From: Gale Limron To: "histonet@lists.utsouthwestern.edu" Sent: Thursday, May 30, 2013 11:26 AM Subject: [Histonet] amyloid stain Hello, It's been awhile since we ran an amyloid stain.............Today, using Richard Allan Chromaview kit, our staining tech noticed a lot of precipitant in the working Congo Red solution. Any thoughts or advice? Thanks, Gale Gale Limron, HT, CT (ASCP) Union Hospital Dover, Oh This e-mail is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential or otherwise protected from disclosure. Dissemination, distribution or copying of this e-mail or the information herein by anyone other than the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, is prohibited. If you received this message in error, please delete without copying and kindly e-mail a reply to inform us of the mistake in delivery. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Thu May 30 10:36:47 2013 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Thu May 30 10:36:52 2013 Subject: [Histonet] Extra Money In-Reply-To: <3a6b3dd518c0423ca60e7f40a74c8f1c@BLUPR07MB001.namprd07.prod.outlook.com> References: , , <1369859801.99883.YahooMailNeo@web163103.mail.bf1.yahoo.com>, <3a6b3dd518c0423ca60e7f40a74c8f1c@BLUPR07MB001.namprd07.prod.outlook.com> Message-ID: Maybe to the nice folks at NSH :) Joelle Weaver MAOM, HTL (ASCP) QIHC > From: sdysart@mirnarx.com > To: rjbuesa@yahoo.com; joelleweaver@hotmail.com; histonet@lists.utsouthwestern.edu > Date: Wed, 29 May 2013 21:05:02 +0000 > Subject: RE: [Histonet] Extra Money > CC: > > I plan on doing a poster for NSH...does that count =) > > Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) > Histotechnologist > Mirna Therapeutics > 2150 Woodward Street > Suite 100 > Austin, Texas 78744 > (512)901-0900 ext. 6912 > > From: Rene J Buesa [mailto:rjbuesa@yahoo.com] > Sent: Wednesday, May 29, 2013 3:37 PM > To: joelle weaver; Sarah Dysart; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Extra Money > > Not even that! If you publish you will build a better c.v. but that is not assurance tof being appreciated by any prospective employer. > In the rare event that it is appreciated you will be able to negotiate a better salary, but that is all! > Ren? J. > > From: joelle weaver > To: Sarah Dysart ; "histonet@lists.utsouthwestern.edu" > Sent: Wednesday, May 29, 2013 4:31 PM > Subject: RE: [Histonet] Extra Money > > Publish > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > From: sdysart@mirnarx.com > > To: histonet@lists.utsouthwestern.edu > > Date: Wed, 29 May 2013 20:20:10 +0000 > > Subject: [Histonet] Extra Money > > > > Anyone know any ideas on how to make a couple extra bucks a month using our histology-awesomeness?? > > > > > > Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) > > Histotechnologist > > Mirna Therapeutics > > 2150 Woodward Street > > Suite 100 > > Austin, Texas 78744 > > (512)901-0900 ext. 6912 > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Timothy.Morken <@t> ucsfmedctr.org Thu May 30 10:52:50 2013 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Thu May 30 10:53:01 2013 Subject: [Histonet] Extra Money In-Reply-To: References: , , <1369859801.99883.YahooMailNeo@web163103.mail.bf1.yahoo.com>, <3a6b3dd518c0423ca60e7f40a74c8f1c@BLUPR07MB001.namprd07.prod.outlook.com> Message-ID: <761E2B5697F795489C8710BCC72141FF081F73@ex07.net.ucsf.edu> Actually it could be like "earning" money because I believe a poster presenter gets a free trip to the NSH meeting. Workshop presenters certainly do. Why do you think there are so many repeat presenters!?!? Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelle weaver Sent: Thursday, May 30, 2013 8:37 AM To: Sarah Dysart; Rene J Buesa; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Extra Money Maybe to the nice folks at NSH :) Joelle Weaver MAOM, HTL (ASCP) QIHC > From: sdysart@mirnarx.com > To: rjbuesa@yahoo.com; joelleweaver@hotmail.com; > histonet@lists.utsouthwestern.edu > Date: Wed, 29 May 2013 21:05:02 +0000 > Subject: RE: [Histonet] Extra Money > CC: > > I plan on doing a poster for NSH...does that count =) > > Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna > Therapeutics > 2150 Woodward Street > Suite 100 > Austin, Texas 78744 > (512)901-0900 ext. 6912 > > From: Rene J Buesa [mailto:rjbuesa@yahoo.com] > Sent: Wednesday, May 29, 2013 3:37 PM > To: joelle weaver; Sarah Dysart; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Extra Money > > Not even that! If you publish you will build a better c.v. but that is not assurance tof being appreciated by any prospective employer. > In the rare event that it is appreciated you will be able to negotiate a better salary, but that is all! > Ren? J. > > From: joelle weaver > To: Sarah Dysart ; > "histonet@lists.utsouthwestern.edu" > > Sent: Wednesday, May 29, 2013 4:31 PM > Subject: RE: [Histonet] Extra Money > > Publish > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > From: sdysart@mirnarx.com > > To: > > histonet@lists.utsouthwestern.edu > rn.edu> > > Date: Wed, 29 May 2013 20:20:10 +0000 > > Subject: [Histonet] Extra Money > > > > Anyone know any ideas on how to make a couple extra bucks a month using our histology-awesomeness?? > > > > > > Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist > > Mirna Therapeutics > > 2150 Woodward Street > > Suite 100 > > Austin, Texas 78744 > > (512)901-0900 ext. 6912 > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > rn.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu .edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Thu May 30 11:17:46 2013 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Thu May 30 11:17:50 2013 Subject: [Histonet] Extra Money In-Reply-To: <761E2B5697F795489C8710BCC72141FF081F73@ex07.net.ucsf.edu> References: , , , , <1369859801.99883.YahooMailNeo@web163103.mail.bf1.yahoo.com>, , <3a6b3dd518c0423ca60e7f40a74c8f1c@BLUPR07MB001.namprd07.prod.outlook.com>, , <761E2B5697F795489C8710BCC72141FF081F73@ex07.net.ucsf.edu> Message-ID: You still have to be able to be off from your regular job- even if it doesn't cost you- that always seems to be the issue for me. Maybe it will happen soon? Joelle Weaver MAOM, HTL (ASCP) QIHC > From: Timothy.Morken@ucsfmedctr.org > To: joelleweaver@hotmail.com; sdysart@mirnarx.com; rjbuesa@yahoo.com; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Extra Money > Date: Thu, 30 May 2013 15:52:50 +0000 > > Actually it could be like "earning" money because I believe a poster presenter gets a free trip to the NSH meeting. Workshop presenters certainly do. Why do you think there are so many repeat presenters!?!? > > > Tim Morken > Supervisor, Electron Microscopy/Neuromuscular Special Studies > Department of Pathology > UC San Francisco Medical Center > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelle weaver > Sent: Thursday, May 30, 2013 8:37 AM > To: Sarah Dysart; Rene J Buesa; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Extra Money > > Maybe to the nice folks at NSH :) > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > From: sdysart@mirnarx.com > > To: rjbuesa@yahoo.com; joelleweaver@hotmail.com; > > histonet@lists.utsouthwestern.edu > > Date: Wed, 29 May 2013 21:05:02 +0000 > > Subject: RE: [Histonet] Extra Money > > CC: > > > > I plan on doing a poster for NSH...does that count =) > > > > Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna > > Therapeutics > > 2150 Woodward Street > > Suite 100 > > Austin, Texas 78744 > > (512)901-0900 ext. 6912 > > > > From: Rene J Buesa [mailto:rjbuesa@yahoo.com] > > Sent: Wednesday, May 29, 2013 3:37 PM > > To: joelle weaver; Sarah Dysart; histonet@lists.utsouthwestern.edu > > Subject: Re: [Histonet] Extra Money > > > > Not even that! If you publish you will build a better c.v. but that is not assurance tof being appreciated by any prospective employer. > > In the rare event that it is appreciated you will be able to negotiate a better salary, but that is all! > > Ren? J. > > > > From: joelle weaver > > To: Sarah Dysart ; > > "histonet@lists.utsouthwestern.edu" > > > > Sent: Wednesday, May 29, 2013 4:31 PM > > Subject: RE: [Histonet] Extra Money > > > > Publish > > > > > > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > > > From: sdysart@mirnarx.com > > > To: > > > histonet@lists.utsouthwestern.edu > > rn.edu> > > > Date: Wed, 29 May 2013 20:20:10 +0000 > > > Subject: [Histonet] Extra Money > > > > > > Anyone know any ideas on how to make a couple extra bucks a month using our histology-awesomeness?? > > > > > > > > > Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist > > > Mirna Therapeutics > > > 2150 Woodward Street > > > Suite 100 > > > Austin, Texas 78744 > > > (512)901-0900 ext. 6912 > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > rn.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > .edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Timothy.Morken <@t> ucsfmedctr.org Thu May 30 11:23:41 2013 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Thu May 30 11:23:53 2013 Subject: [Histonet] Extra Money In-Reply-To: References: , , , , <1369859801.99883.YahooMailNeo@web163103.mail.bf1.yahoo.com>, , <3a6b3dd518c0423ca60e7f40a74c8f1c@BLUPR07MB001.namprd07.prod.outlook.com>, , <761E2B5697F795489C8710BCC72141FF081F73@ex07.net.ucsf.edu> Message-ID: <761E2B5697F795489C8710BCC72141FF081FC4@ex07.net.ucsf.edu> Do you mean workload wise, or employer-permission wise? In either case I HOPE that an employer would be willing to give you vacation (or better yet, educational leave) to go to a national meeting for which you will present a project or workshop relating to your job. That is a dream employee! Using temp labor for such a purpose is a no-brainer for employee morale, retention and value to any company. Tim From: joelle weaver [mailto:joelleweaver@hotmail.com] Sent: Thursday, May 30, 2013 9:18 AM To: Morken, Timothy; Sarah Dysart; Rene J Buesa; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Extra Money You still have to be able to be off from your regular job- even if it doesn't cost you- that always seems to be the issue for me. Maybe it will happen soon? Joelle Weaver MAOM, HTL (ASCP) QIHC > From: Timothy.Morken@ucsfmedctr.org > To: joelleweaver@hotmail.com; sdysart@mirnarx.com; rjbuesa@yahoo.com; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Extra Money > Date: Thu, 30 May 2013 15:52:50 +0000 > > Actually it could be like "earning" money because I believe a poster presenter gets a free trip to the NSH meeting. Workshop presenters certainly do. Why do you think there are so many repeat presenters!?!? > > > Tim Morken > Supervisor, Electron Microscopy/Neuromuscular Special Studies > Department of Pathology > UC San Francisco Medical Center > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelle weaver > Sent: Thursday, May 30, 2013 8:37 AM > To: Sarah Dysart; Rene J Buesa; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Extra Money > > Maybe to the nice folks at NSH :) > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > From: sdysart@mirnarx.com > > To: rjbuesa@yahoo.com; joelleweaver@hotmail.com; > > histonet@lists.utsouthwestern.edu > > Date: Wed, 29 May 2013 21:05:02 +0000 > > Subject: RE: [Histonet] Extra Money > > CC: > > > > I plan on doing a poster for NSH...does that count =) > > > > Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna > > Therapeutics > > 2150 Woodward Street > > Suite 100 > > Austin, Texas 78744 > > (512)901-0900 ext. 6912 > > > > From: Rene J Buesa [mailto:rjbuesa@yahoo.com] > > Sent: Wednesday, May 29, 2013 3:37 PM > > To: joelle weaver; Sarah Dysart; histonet@lists.utsouthwestern.edu > > Subject: Re: [Histonet] Extra Money > > > > Not even that! If you publish you will build a better c.v. but that is not assurance tof being appreciated by any prospective employer. > > In the rare event that it is appreciated you will be able to negotiate a better salary, but that is all! > > Ren? J. > > > > From: joelle weaver > > > To: Sarah Dysart >; > > "histonet@lists.utsouthwestern.edu" > > > > > Sent: Wednesday, May 29, 2013 4:31 PM > > Subject: RE: [Histonet] Extra Money > > > > Publish > > > > > > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > > > From: sdysart@mirnarx.com> > > > To: > > > histonet@lists.utsouthwestern.edu > > > rn.edu> > > > Date: Wed, 29 May 2013 20:20:10 +0000 > > > Subject: [Histonet] Extra Money > > > > > > Anyone know any ideas on how to make a couple extra bucks a month using our histology-awesomeness?? > > > > > > > > > Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist > > > Mirna Therapeutics > > > 2150 Woodward Street > > > Suite 100 > > > Austin, Texas 78744 > > > (512)901-0900 ext. 6912 > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > rn.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > .edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From TNMayer <@t> mdanderson.org Thu May 30 11:29:40 2013 From: TNMayer <@t> mdanderson.org (Mayer,Toysha N) Date: Thu May 30 11:33:37 2013 Subject: [Histonet] RE: Extra money Message-ID: <47E9B2C01DDDD94881EACD2DC44EBC880160A73C@D1PWPEXMBX05.mdanderson.edu> Hey Sarah, Try the local Community college. They may have a need for someone to help with labs on weekends. Also, try a, god forbid I say this, POD labs. They are growing the area and may need the extra expertise. The Clinical Research companies in the area may also be looking. I'm surprised CPL isn't looking for a pt tech, they usually have openings. Good luck Toysha N. Mayer, MBA, HT (ASCP) Instructor, Education Coordinator Program in Histotechnology School of Health Professions MD Anderson Cancer Center (713) 563-3481 tnmayer@mdanderson.org Message: 5 Date: Wed, 29 May 2013 20:20:10 +0000 From: Sarah Dysart Subject: [Histonet] Extra Money To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Anyone know any ideas on how to make a couple extra bucks a month using our histology-awesomeness?? Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 From joelleweaver <@t> hotmail.com Thu May 30 11:40:22 2013 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Thu May 30 11:40:31 2013 Subject: [Histonet] Extra Money In-Reply-To: <761E2B5697F795489C8710BCC72141FF081FC4@ex07.net.ucsf.edu> References: , , , , <1369859801.99883.YahooMailNeo@web163103.mail.bf1.yahoo.com>, , <3a6b3dd518c0423ca60e7f40a74c8f1c@BLUPR07MB001.namprd07.prod.outlook.com>, , <761E2B5697F795489C8710BCC72141FF081F73@ex07.net.ucsf.edu>, , <761E2B5697F795489C8710BCC72141FF081FC4@ex07.net.ucsf.edu> Message-ID: Many reasons over the years. Usually staffing issues. You have a nice attitude about it, I'm sure your employees appreciate that. Joelle Weaver MAOM, HTL (ASCP) QIHC From: Timothy.Morken@ucsfmedctr.org To: joelleweaver@hotmail.com; sdysart@mirnarx.com; rjbuesa@yahoo.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Extra Money Date: Thu, 30 May 2013 16:23:41 +0000 Do you mean workload wise, or employer-permission wise? In either case I HOPE that an employer would be willing to give you vacation (or better yet, educational leave) to go to a national meeting for which you will present a project or workshop relating to your job. That is a dream employee! Using temp labor for such a purpose is a no-brainer for employee morale, retention and value to any company. Tim From: joelle weaver [mailto:joelleweaver@hotmail.com] Sent: Thursday, May 30, 2013 9:18 AM To: Morken, Timothy; Sarah Dysart; Rene J Buesa; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Extra Money You still have to be able to be off from your regular job- even if it doesn't cost you- that always seems to be the issue for me. Maybe it will happen soon? Joelle Weaver MAOM, HTL (ASCP) QIHC > From: Timothy.Morken@ucsfmedctr.org > To: joelleweaver@hotmail.com; sdysart@mirnarx.com; rjbuesa@yahoo.com; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Extra Money > Date: Thu, 30 May 2013 15:52:50 +0000 > > Actually it could be like "earning" money because I believe a poster presenter gets a free trip to the NSH meeting. Workshop presenters certainly do. Why do you think there are so many repeat presenters!?!? > > > Tim Morken > Supervisor, Electron Microscopy/Neuromuscular Special Studies > Department of Pathology > UC San Francisco Medical Center > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelle weaver > Sent: Thursday, May 30, 2013 8:37 AM > To: Sarah Dysart; Rene J Buesa; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Extra Money > > Maybe to the nice folks at NSH :) > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > From: sdysart@mirnarx.com > > To: rjbuesa@yahoo.com; joelleweaver@hotmail.com; > > histonet@lists.utsouthwestern.edu > > Date: Wed, 29 May 2013 21:05:02 +0000 > > Subject: RE: [Histonet] Extra Money > > CC: > > > > I plan on doing a poster for NSH...does that count =) > > > > Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna > > Therapeutics > > 2150 Woodward Street > > Suite 100 > > Austin, Texas 78744 > > (512)901-0900 ext. 6912 > > > > From: Rene J Buesa [mailto:rjbuesa@yahoo.com] > > Sent: Wednesday, May 29, 2013 3:37 PM > > To: joelle weaver; Sarah Dysart; histonet@lists.utsouthwestern.edu > > Subject: Re: [Histonet] Extra Money > > > > Not even that! If you publish you will build a better c.v. but that is not assurance tof being appreciated by any prospective employer. > > In the rare event that it is appreciated you will be able to negotiate a better salary, but that is all! > > Ren? J. > > > > From: joelle weaver > > To: Sarah Dysart ; > > "histonet@lists.utsouthwestern.edu" > > > > Sent: Wednesday, May 29, 2013 4:31 PM > > Subject: RE: [Histonet] Extra Money > > > > Publish > > > > > > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > > > From: sdysart@mirnarx.com > > > To: > > > histonet@lists.utsouthwestern.edu > > rn.edu> > > > Date: Wed, 29 May 2013 20:20:10 +0000 > > > Subject: [Histonet] Extra Money > > > > > > Anyone know any ideas on how to make a couple extra bucks a month using our histology-awesomeness?? > > > > > > > > > Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist > > > Mirna Therapeutics > > > 2150 Woodward Street > > > Suite 100 > > > Austin, Texas 78744 > > > (512)901-0900 ext. 6912 > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > rn.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > .edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From ttruscot <@t> vetmed.wsu.edu Thu May 30 10:46:54 2013 From: ttruscot <@t> vetmed.wsu.edu (Truscott, Tom) Date: Thu May 30 11:43:28 2013 Subject: [Histonet] Processing thin slices of mouse cerebellum In-Reply-To: <1fd19caf86a3f5b9bb14e12677813a2b.squirrel@webmail.rci.rutgers.edu> References: <1fd19caf86a3f5b9bb14e12677813a2b.squirrel@webmail.rci.rutgers.edu> Message-ID: <9EF5279EBDFE6E4FB6605E8F183A00275BAF9A0C@CVM76.vetmed.wsu.edu> Hi Kathleen, Several years and a couple employers ago we attached small biopsies (for proper orientation) to small squares of cucumber (which had been dehydrated in several changes of alcohol) with a egg-albumin/glycerol mixture. The bx/cucumber unit was embedded together after processing and cut and stained as a unit with no adverse effects. Tom Truscott -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kgrobert@rci.rutgers.edu Sent: Wednesday, May 29, 2013 11:14 AM To: histonet Subject: [Histonet] Processing thin slices of mouse cerebellum To all, We have these very thin slices of mouse cerebellum that need to be processed into paraffin. The problem is that they need to be kept flat. We have tried sponges, but some of the slices are so small that they shrink (processed with our biopsy program, of course; if you want details, let me know) and get lost in the holes of the sponges. We have biopsy cassettes, but the slices will still tumble and not stay flat. We have processed other things in lens paper, but I had to scrape the tissue with the paraffin into the mold. I have not tried Histogel, though-maybe embed the slices flat in that first and then process it? Is there anything else? Thanks so much! Kathleen Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology & Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (848) 445-1443 FAX (732) 445-6905 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Thu May 30 11:48:00 2013 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Thu May 30 11:48:13 2013 Subject: [Histonet] Extra Money In-Reply-To: References: , , , , <1369859801.99883.YahooMailNeo@web163103.mail.bf1.yahoo.com>, , <3a6b3dd518c0423ca60e7f40a74c8f1c@BLUPR07MB001.namprd07.prod.outlook.com>, , <761E2B5697F795489C8710BCC72141FF081F73@ex07.net.ucsf.edu>, , <761E2B5697F795489C8710BCC72141FF081FC4@ex07.net.ucsf.edu> Message-ID: <761E2B5697F795489C8710BCC72141FF081FFE@ex07.net.ucsf.edu> Well, here it helps we are a large institution that does give education leave and we do a lot of cross training and have many per diems available. Smaller labs have a much tougher situation. Been there! Tim From: joelle weaver [mailto:joelleweaver@hotmail.com] Sent: Thursday, May 30, 2013 9:40 AM To: Morken, Timothy; Sarah Dysart; Rene J Buesa; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Extra Money Many reasons over the years. Usually staffing issues. You have a nice attitude about it, I'm sure your employees appreciate that. Joelle Weaver MAOM, HTL (ASCP) QIHC ________________________________ From: Timothy.Morken@ucsfmedctr.org To: joelleweaver@hotmail.com; sdysart@mirnarx.com; rjbuesa@yahoo.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Extra Money Date: Thu, 30 May 2013 16:23:41 +0000 Do you mean workload wise, or employer-permission wise? In either case I HOPE that an employer would be willing to give you vacation (or better yet, educational leave) to go to a national meeting for which you will present a project or workshop relating to your job. That is a dream employee! Using temp labor for such a purpose is a no-brainer for employee morale, retention and value to any company. Tim From: joelle weaver [mailto:joelleweaver@hotmail.com] Sent: Thursday, May 30, 2013 9:18 AM To: Morken, Timothy; Sarah Dysart; Rene J Buesa; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Extra Money You still have to be able to be off from your regular job- even if it doesn't cost you- that always seems to be the issue for me. Maybe it will happen soon? Joelle Weaver MAOM, HTL (ASCP) QIHC > From: Timothy.Morken@ucsfmedctr.org > To: joelleweaver@hotmail.com; sdysart@mirnarx.com; rjbuesa@yahoo.com; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Extra Money > Date: Thu, 30 May 2013 15:52:50 +0000 > > Actually it could be like "earning" money because I believe a poster presenter gets a free trip to the NSH meeting. Workshop presenters certainly do. Why do you think there are so many repeat presenters!?!? > > > Tim Morken > Supervisor, Electron Microscopy/Neuromuscular Special Studies > Department of Pathology > UC San Francisco Medical Center > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelle weaver > Sent: Thursday, May 30, 2013 8:37 AM > To: Sarah Dysart; Rene J Buesa; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Extra Money > > Maybe to the nice folks at NSH :) > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > From: sdysart@mirnarx.com > > To: rjbuesa@yahoo.com; joelleweaver@hotmail.com; > > histonet@lists.utsouthwestern.edu > > Date: Wed, 29 May 2013 21:05:02 +0000 > > Subject: RE: [Histonet] Extra Money > > CC: > > > > I plan on doing a poster for NSH...does that count =) > > > > Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna > > Therapeutics > > 2150 Woodward Street > > Suite 100 > > Austin, Texas 78744 > > (512)901-0900 ext. 6912 > > > > From: Rene J Buesa [mailto:rjbuesa@yahoo.com] > > Sent: Wednesday, May 29, 2013 3:37 PM > > To: joelle weaver; Sarah Dysart; histonet@lists.utsouthwestern.edu > > Subject: Re: [Histonet] Extra Money > > > > Not even that! If you publish you will build a better c.v. but that is not assurance tof being appreciated by any prospective employer. > > In the rare event that it is appreciated you will be able to negotiate a better salary, but that is all! > > Ren? J. > > > > From: joelle weaver > > > To: Sarah Dysart >; > > "histonet@lists.utsouthwestern.edu" > > > > > Sent: Wednesday, May 29, 2013 4:31 PM > > Subject: RE: [Histonet] Extra Money > > > > Publish > > > > > > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > > > From: sdysart@mirnarx.com> > > > To: > > > histonet@lists.utsouthwestern.edu > > > rn.edu> > > > Date: Wed, 29 May 2013 20:20:10 +0000 > > > Subject: [Histonet] Extra Money > > > > > > Anyone know any ideas on how to make a couple extra bucks a month using our histology-awesomeness?? > > > > > > > > > Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist > > > Mirna Therapeutics > > > 2150 Woodward Street > > > Suite 100 > > > Austin, Texas 78744 > > > (512)901-0900 ext. 6912 > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > rn.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > .edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From sdysart <@t> mirnarx.com Thu May 30 11:54:13 2013 From: sdysart <@t> mirnarx.com (Sarah Dysart) Date: Thu May 30 11:54:21 2013 Subject: [Histonet] Extra Money In-Reply-To: <761E2B5697F795489C8710BCC72141FF081F73@ex07.net.ucsf.edu> References: , , <1369859801.99883.YahooMailNeo@web163103.mail.bf1.yahoo.com>, <3a6b3dd518c0423ca60e7f40a74c8f1c@BLUPR07MB001.namprd07.prod.outlook.com> <761E2B5697F795489C8710BCC72141FF081F73@ex07.net.ucsf.edu> Message-ID: <959e87134bcb4fa589df6d2697151d77@BLUPR07MB001.namprd07.prod.outlook.com> Well if that's the case I just spent a lot of my companies money for nothing... Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: Morken, Timothy [mailto:Timothy.Morken@ucsfmedctr.org] Sent: Thursday, May 30, 2013 10:53 AM To: joelle weaver; Sarah Dysart; Rene J Buesa; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Extra Money Actually it could be like "earning" money because I believe a poster presenter gets a free trip to the NSH meeting. Workshop presenters certainly do. Why do you think there are so many repeat presenters!?!? Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelle weaver Sent: Thursday, May 30, 2013 8:37 AM To: Sarah Dysart; Rene J Buesa; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Extra Money Maybe to the nice folks at NSH :) Joelle Weaver MAOM, HTL (ASCP) QIHC > From: sdysart@mirnarx.com > To: rjbuesa@yahoo.com; joelleweaver@hotmail.com; > histonet@lists.utsouthwestern.edu > Date: Wed, 29 May 2013 21:05:02 +0000 > Subject: RE: [Histonet] Extra Money > CC: > > I plan on doing a poster for NSH...does that count =) > > Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna > Therapeutics > 2150 Woodward Street > Suite 100 > Austin, Texas 78744 > (512)901-0900 ext. 6912 > > From: Rene J Buesa [mailto:rjbuesa@yahoo.com] > Sent: Wednesday, May 29, 2013 3:37 PM > To: joelle weaver; Sarah Dysart; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Extra Money > > Not even that! If you publish you will build a better c.v. but that is not assurance tof being appreciated by any prospective employer. > In the rare event that it is appreciated you will be able to negotiate a better salary, but that is all! > Ren? J. > > From: joelle weaver > To: Sarah Dysart ; > "histonet@lists.utsouthwestern.edu" > > Sent: Wednesday, May 29, 2013 4:31 PM > Subject: RE: [Histonet] Extra Money > > Publish > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > From: sdysart@mirnarx.com > > To: > > histonet@lists.utsouthwestern.edu > rn.edu> > > Date: Wed, 29 May 2013 20:20:10 +0000 > > Subject: [Histonet] Extra Money > > > > Anyone know any ideas on how to make a couple extra bucks a month using our histology-awesomeness?? > > > > > > Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist > > Mirna Therapeutics > > 2150 Woodward Street > > Suite 100 > > Austin, Texas 78744 > > (512)901-0900 ext. 6912 > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > rn.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu .edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> gmail.com Thu May 30 12:27:02 2013 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Thu May 30 12:27:07 2013 Subject: [Histonet] Re: Processing thin slices of mouse cerebellum Message-ID: Tom Truscott replies to Kathleen [Robert]: >>Several years and a couple employers ago we attached small biopsies (for proper orientation) to small squares of cucumber (which had been dehydrated in several changes of alcohol) with a egg-albumin/glycerol mixture. The bx/cucumber unit was embedded together after processing and cut and stained as a unit with no adverse effects.<< I never saw it, but this cucumber technique was in fairly common use among gynecologic pathologists 40 or 50 years ago, for embedding cervical biopsy specimens. The cucumber in alcohol was provided to the GYN performing the biopsy. I think I'd prefer a gin & tonic. Bob Richmond Samurai Pathologist Maryville TN From JMitchell <@t> uwhealth.org Thu May 30 14:12:04 2013 From: JMitchell <@t> uwhealth.org (Mitchell Jean A) Date: Thu May 30 14:12:10 2013 Subject: [Histonet] Extra Money In-Reply-To: <761E2B5697F795489C8710BCC72141FF081F73@ex07.net.ucsf.edu> References: , , <1369859801.99883.YahooMailNeo@web163103.mail.bf1.yahoo.com>, <3a6b3dd518c0423ca60e7f40a74c8f1c@BLUPR07MB001.namprd07.prod.outlook.com> <761E2B5697F795489C8710BCC72141FF081F73@ex07.net.ucsf.edu> Message-ID: <16F90B93CA23D446980B3D591FD02DAD05F4BF@UWHC-MBX14.uwhis.hosp.wisc.edu> To clarify: Winning NSH poster presentations in both Clinical and VIR categories each receive $250.00 - but NSH does not reimburse any expenses for poster presentation. Hope no one got their hopes up on this.... Jean Mitchell -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Thursday, May 30, 2013 10:53 AM To: joelle weaver; Sarah Dysart; Rene J Buesa; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Extra Money Actually it could be like "earning" money because I believe a poster presenter gets a free trip to the NSH meeting. Workshop presenters certainly do. Why do you think there are so many repeat presenters!?!? Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelle weaver Sent: Thursday, May 30, 2013 8:37 AM To: Sarah Dysart; Rene J Buesa; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Extra Money Maybe to the nice folks at NSH :) Joelle Weaver MAOM, HTL (ASCP) QIHC > From: sdysart@mirnarx.com > To: rjbuesa@yahoo.com; joelleweaver@hotmail.com; > histonet@lists.utsouthwestern.edu > Date: Wed, 29 May 2013 21:05:02 +0000 > Subject: RE: [Histonet] Extra Money > CC: > > I plan on doing a poster for NSH...does that count =) > > Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna > Therapeutics > 2150 Woodward Street > Suite 100 > Austin, Texas 78744 > (512)901-0900 ext. 6912 > > From: Rene J Buesa [mailto:rjbuesa@yahoo.com] > Sent: Wednesday, May 29, 2013 3:37 PM > To: joelle weaver; Sarah Dysart; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Extra Money > > Not even that! If you publish you will build a better c.v. but that is not assurance tof being appreciated by any prospective employer. > In the rare event that it is appreciated you will be able to negotiate a better salary, but that is all! > Ren? J. > > From: joelle weaver > To: Sarah Dysart ; > "histonet@lists.utsouthwestern.edu" > > Sent: Wednesday, May 29, 2013 4:31 PM > Subject: RE: [Histonet] Extra Money > > Publish > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > From: sdysart@mirnarx.com > > To: > > histonet@lists.utsouthwestern.edu > rn.edu> > > Date: Wed, 29 May 2013 20:20:10 +0000 > > Subject: [Histonet] Extra Money > > > > Anyone know any ideas on how to make a couple extra bucks a month using our histology-awesomeness?? > > > > > > Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist > > Mirna Therapeutics > > 2150 Woodward Street > > Suite 100 > > Austin, Texas 78744 > > (512)901-0900 ext. 6912 > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > rn.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu .edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From McKenzie.Emily <@t> mhsil.com Thu May 30 14:59:23 2013 From: McKenzie.Emily <@t> mhsil.com (McKenzie, Emily) Date: Thu May 30 14:59:27 2013 Subject: [Histonet] HT vs. HTL Message-ID: Can anyone provide job descriptions for HT vs. HTL. We are looking to separated the two positions. If there are any suggestions please let me know. Thank you, Emily McKenzie Memorial Medical Center ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From shive003 <@t> umn.edu Thu May 30 15:08:33 2013 From: shive003 <@t> umn.edu (Jan Shivers) Date: Thu May 30 15:08:37 2013 Subject: [Histonet] antigen retrieval question In-Reply-To: References: Message-ID: Lynn, I use the Decloaker and also stain for BVD, so will get in contact with you later this afternoon. Jan Shivers University of Minnesota Veterinary Diagnostic Lab St. Paul, MN 55108 On Thu, May 30, 2013 at 9:54 AM, Burton, Lynn wrote: > If anyone out there is using a decloaker for antigen retrieval, when doing > ihc staining, would you please contact me on my e-mail address? I have been > struggling with a BVD test for months. It is not working consistently and I > want to explore using this method along with our Benchmark XT stainer from > Ventana. > Thank you, > Lynn Burton > Galesburg Animal Disease Lab > Galesburg, Il > lynn.burton@illinois.gov > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From lpwenk <@t> sbcglobal.net Thu May 30 18:28:40 2013 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Thu May 30 18:28:46 2013 Subject: [Histonet] Extra Money In-Reply-To: <761E2B5697F795489C8710BCC72141FF081F73@ex07.net.ucsf.edu> References: , , <1369859801.99883.YahooMailNeo@web163103.mail.bf1.yahoo.com>, <3a6b3dd518c0423ca60e7f40a74c8f1c@BLUPR07MB001.namprd07.prod.outlook.com> <761E2B5697F795489C8710BCC72141FF081F73@ex07.net.ucsf.edu> Message-ID: The poster reimbursement has been addressed. So I'll talk about workshop presenters. It's not a free trip, but some of it is paid. For presenting 1 workshop, you are reimbursed: - air fare or cost of driving, whichever is least - parking at airport (if flying) or at hotel (if driving) - 2 nights of hotel (not to exceed the "convention" hotel rate). You pay for the extra days of hotel you are there. - registration fee ($35), but you have to pay for any workshops you want to attend - 1 banquet ticket - meals for 2 days (I think it's about $35/day). If you know the cost of food at convention hotels, $35 MIGHT cover part of breakfast and lunch. If you are over the $35, you pay for the rest. If you are there more than 2 days, you pay for your own food. So presenting does not give you a free ride to the convention, but it does help reduce the amount that you pay to attend. If anyone is interested in presenting, it works best if you have presented several times at your state meeting/symposium, and start building up a good reputation as a good presenter. NSH usually does not ask a "first time presenter" to talk at a national symposium. If you have no state organization, ask NSH to help you start one. Or, start presenting at in-services where you work. Don't have any of those? Start a monthly in-service, and put that on your resume/CV. (yes, you will need a curriculum vitae to submit to NSH if accepted.) Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: Morken, Timothy Sent: Thursday, May 30, 2013 11:52 AM To: joelle weaver ; Sarah Dysart ; Rene J Buesa ; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Extra Money Actually it could be like "earning" money because I believe a poster presenter gets a free trip to the NSH meeting. Workshop presenters certainly do. Why do you think there are so many repeat presenters!?!? Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelle weaver Sent: Thursday, May 30, 2013 8:37 AM To: Sarah Dysart; Rene J Buesa; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Extra Money Maybe to the nice folks at NSH :) Joelle Weaver MAOM, HTL (ASCP) QIHC > From: sdysart@mirnarx.com > To: rjbuesa@yahoo.com; joelleweaver@hotmail.com; > histonet@lists.utsouthwestern.edu > Date: Wed, 29 May 2013 21:05:02 +0000 > Subject: RE: [Histonet] Extra Money > CC: > > I plan on doing a poster for NSH...does that count =) > > Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna > Therapeutics > 2150 Woodward Street > Suite 100 > Austin, Texas 78744 > (512)901-0900 ext. 6912 > > From: Rene J Buesa [mailto:rjbuesa@yahoo.com] > Sent: Wednesday, May 29, 2013 3:37 PM > To: joelle weaver; Sarah Dysart; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Extra Money > > Not even that! If you publish you will build a better c.v. but that is not > assurance tof being appreciated by any prospective employer. > In the rare event that it is appreciated you will be able to negotiate a > better salary, but that is all! > Ren? J. > > From: joelle weaver > To: Sarah Dysart ; > "histonet@lists.utsouthwestern.edu" > > Sent: Wednesday, May 29, 2013 4:31 PM > Subject: RE: [Histonet] Extra Money > > Publish > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > From: sdysart@mirnarx.com > > To: > > histonet@lists.utsouthwestern.edu > rn.edu> > > Date: Wed, 29 May 2013 20:20:10 +0000 > > Subject: [Histonet] Extra Money > > > > Anyone know any ideas on how to make a couple extra bucks a month using > > our histology-awesomeness?? > > > > > > Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist > > Mirna Therapeutics > > 2150 Woodward Street > > Suite 100 > > Austin, Texas 78744 > > (512)901-0900 ext. 6912 > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > rn.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu .edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carl.hobbs <@t> kcl.ac.uk Fri May 31 02:30:45 2013 From: carl.hobbs <@t> kcl.ac.uk (Hobbs, Carl) Date: Fri May 31 02:31:21 2013 Subject: [Histonet] Processing thin slices of mouse cerebellum Message-ID: <00D6B8253EAED840B8D04E235497381831FF23AD@AM2PRD0311MB399.eurprd03.prod.outlook.com> http://www.cellpath.us.com/magento/cellpath-on-line-shop/specimen-processing-and-embedding/small-biospy-processing/cellsafe/cellsafe-biopsy-capsule-blue.html I find them very good for brain slices. There is a deeper and a shallower space, depending on which way they are folded. Carl Hobbs FIBMS Histology and Imaging Manager Wolfson Centre for Age-Related Diseases School of Biomedical Sciences King's College London Guys Campus London SE1 1UL ? Tel.020 7848 6810 fax 020 7848 6816 carl.hobbs@kcl.ac.uk From BMolinari <@t> texasheart.org Fri May 31 06:00:07 2013 From: BMolinari <@t> texasheart.org (Molinari, Betsy) Date: Fri May 31 06:00:16 2013 Subject: [Histonet] Movats problem solved Message-ID: Good Friday to all. Histology 101. The Alcian Blue dye itself had lost its efficacy. I borrowed a solution from another lab and voila, perfect staining. Thank you to everyone for their suggestions. I will certainly keep them on file in the event this ever happens again. Have a good weekend. Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 1101 Bates St. Houston,TX 77030 832-355-6524 (lab) 832-355-6812 (fax) Betsy Molinari Senior Histology Research Technician 832-355-6524 | BMolinari@texasheart.org | www.texasheart.org 6770 Bertner Ave., MC 1-283, Houston, TX 77030 [Texas Heart Institute][THI News] [THI on Facebook] [THI on Flicker] [THI on Google] [THI on Pinterest] [THI on Twitter] [THI on You Tube] Confidentiality Notice: This message may be confidential and/or privileged. If you are not the intended recipient you may not review, disseminate or copy this e-mail, its contents and/or any attachments. Please immediately notify the sender If you have received this e-mail in error and delete it from your computer system. From Colleen_Herring <@t> bshsi.org Fri May 31 06:16:49 2013 From: Colleen_Herring <@t> bshsi.org (Herring, Colleen) Date: Fri May 31 06:16:56 2013 Subject: [Histonet] Steiner stain Message-ID: I am looking for some suggestions on a problem with our steiner stain. We have a percipitate on the whole slide not just on the tissue and no matter what we have done I can't seem to get rid of it. HELP! ________________________________________________________________________________________________________ The information in this communication is intended to be confidential to the Individual(s) and/or Entity to whom it is addressed. It may contain information of a Privileged and/or Confidential nature, which is subject to Federal and/or State privacy regulations. In the event that you are not the intended recipient or the agent of the intended recipient, do not copy or use the information contained within this communication, or allow it to be read, copied or utilized in any manner, by any other person(s). Should this communication be received in error, please notify the sender immediately either by response e-mail or by phone, and permanently delete the original e-mail, attachment(s), and any copies. ________________________________________________________________________________________________________ From m_chadwell <@t> hotmail.com Fri May 31 06:27:58 2013 From: m_chadwell <@t> hotmail.com (margaret chadwell ) Date: Fri May 31 06:28:03 2013 Subject: [Histonet] Autotechnicon processor Message-ID: Does anyone have an Autotechnicon Tissue Processor for sale?Thanks much!Margie Chadwell From rjbuesa <@t> yahoo.com Fri May 31 09:11:29 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri May 31 09:11:34 2013 Subject: [Histonet] Steiner stain In-Reply-To: References: Message-ID: <1370009489.5987.YahooMailNeo@web163106.mail.bf1.yahoo.com> Usually any (and almost all) precipitates when using a silver stain is produced by the silver solution reacting over some remnant of a previous solution not completely removed. Check your solutions, if needed prepare them anew and try again. Ren? J. From: "Herring, Colleen" To: Histonet@lists.utsouthwestern.edu Sent: Friday, May 31, 2013 7:16 AM Subject: [Histonet] Steiner stain I am looking for some suggestions on a problem with our steiner stain. We have a percipitate on the whole slide not just on the tissue and no matter what we have done I can't seem to get rid of it. HELP! ________________________________________________________________________________________________________ The information in this communication is intended to be confidential to the Individual(s) and/or Entity to whom it is addressed. It may contain information of a Privileged and/or Confidential nature, which is subject to Federal and/or State privacy regulations. In the event that you are not the intended recipient or the agent of the intended recipient, do not copy or use the information contained within this communication, or allow it to be read, copied or utilized in any manner, by any other person(s). Should this communication be received in error, please notify the sender immediately either by response e-mail or by phone, and permanently delete the original e-mail, attachment(s), and any copies. ________________________________________________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dmccaig <@t> ckha.on.ca Fri May 31 10:00:57 2013 From: dmccaig <@t> ckha.on.ca (Diana McCaig) Date: Fri May 31 10:01:11 2013 Subject: [Histonet] bone marrow aspirations Message-ID: Can anyone share with me their process for processing bone marrow aspirations and embedding them in paraffin blocks. Currently ours are being spread out throughout the entire base mold and makes it cumbersome to screen. Has anyone used histogel or making a button and embed that instead of just filtering the aspirate and putting that is a cassette. Thanks Diana From Valerie.Hannen <@t> parrishmed.com Fri May 31 10:49:21 2013 From: Valerie.Hannen <@t> parrishmed.com (Hannen, Valerie) Date: Fri May 31 10:49:26 2013 Subject: [Histonet] RE: bone marrow aspirations In-Reply-To: Message-ID: <450B7A81EDA0C54E97C53D60F00776C3232B4BB5E9@isexstore03> Diana, We let the aspirates clot in a petri dish, cover them in formalin to fix the clot and then make a block from the fixed clot. Process overnight in the processor and embed the clot in the A.M. This process helps eliminate the aspirate from spreading out in the base mold. I hope this helps. Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.hannen@parrishmed.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Diana McCaig Sent: Friday, May 31, 2013 11:01 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] bone marrow aspirations Can anyone share with me their process for processing bone marrow aspirations and embedding them in paraffin blocks. Currently ours are being spread out throughout the entire base mold and makes it cumbersome to screen. Has anyone used histogel or making a button and embed that instead of just filtering the aspirate and putting that is a cassette. Thanks Diana _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet =================== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" =================== From Allison.Scott <@t> harrishealth.org Fri May 31 11:31:05 2013 From: Allison.Scott <@t> harrishealth.org (Scott, Allison D) Date: Fri May 31 11:31:17 2013 Subject: [Histonet] Formalin Message-ID: Hello to all in histoland. Happy Friday. Last week we were inspected by a new group of surveyors that are like JCAHO called DNV. They said that we had to much formalin stored in our gross room. We had 15 gallons. They told us that we could only have 2 gallons there at a time. I have never heard of such. We were just inspected by CAP and I might add that it was an intense inspection. They checked everything. This never came up. What is the recommended amount of formalin that can be stored in an area. Does it need to be in a flammable cabinet? The cabinet that we have the formalin in is so old that we are having a vendor to come out to check to see if it suitable for chemical storage. Allison Scott HT(ASCP) Supervisor, Histology Lab LBJ Hospital Harris Health System Office: 713-566-2148 Lab: 713-566-5287 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. From rjbuesa <@t> yahoo.com Fri May 31 11:41:17 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri May 31 11:41:23 2013 Subject: [Histonet] Formalin In-Reply-To: References: Message-ID: <1370018477.61740.YahooMailNeo@web163103.mail.bf1.yahoo.com> Formalin is THEORETICALLY (and very UNLIKELY) "flammable, but putting it into a flammables cabinet is going too far. Try to find out those conflicting standards (JCAHO vs. CAP) and follow the strictest. Also please remember that many inspectors just "love to show off!" Some think that have to "cite for something" because otherwise they are not doing a good job, or do not know enough.? Ren? J. From: "Scott, Allison D" To: "histonet@lists.utsouthwestern.edu" Sent: Friday, May 31, 2013 12:31 PM Subject: [Histonet] Formalin Hello to all in histoland.? Happy Friday.? Last week we were inspected by a new group of surveyors that are like JCAHO called DNV.? They said that we had to much formalin stored in our gross room.? We had 15 gallons.? They told us that we could only have 2 gallons there at a time.? I have never heard of such.? We were just inspected by CAP and I might add that it was an? intense inspection.? They checked everything. This never came up.? What is the recommended amount of formalin that can be stored in an area.? Does it need to be in a flammable cabinet?? The cabinet that we have the formalin in is so old that we are? having a vendor to come out to check to see if it suitable for chemical storage. Allison Scott HT(ASCP) Supervisor, Histology Lab LBJ Hospital Harris Health System Office: 713-566-2148 Lab: 713-566-5287 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged.? This e-mail may also be confidential and/or privileged under Texas law.? The e-mail is for the use of only the individual or entity named above.? If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Fri May 31 12:01:46 2013 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Fri May 31 12:01:49 2013 Subject: [Histonet] RE: Formalin In-Reply-To: References: Message-ID: <14E2C6176416974295479C64A11CB9AE016411AC2EEF@SBS2K8.premierlab.local> Allison I have not heard of that either, that would mean that you could not even store one 5 gallon cube of formalin. I would politely ask them for a copy of the regulation that states that there can only be 2 gallons of formalin stored in your gross room. They may be classifying the formalin as a flammable, which I think may be happening in the future once the US adopts "Global Standards" but I don't believe we are there right now. We keep at most 10 gallons of formalin in the lab. One 5 gallon cube that is opened and then a second cube that has not been opened. NSH had a really nice teleconference recently on Training for Revised Hazard Communication Standard by Ada Feldman. That teleconference was from March 27, 2013. I'm not certain but I believe that if the US adopts these so called "global standards" that formalin would be classified as a flammable as well as quite of few of the other chemicals we use in the histology laboratory. Maybe there is someone out on the Histonet that knows a bit more than me that can comment on this. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Laboratory Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 Work (303) 682-3949 Fax (303) 682-9060 Cell (303) 881-0763 liz@premierlab.com www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Friday, May 31, 2013 10:31 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formalin Hello to all in histoland. Happy Friday. Last week we were inspected by a new group of surveyors that are like JCAHO called DNV. They said that we had to much formalin stored in our gross room. We had 15 gallons. They told us that we could only have 2 gallons there at a time. I have never heard of such. We were just inspected by CAP and I might add that it was an intense inspection. They checked everything. This never came up. What is the recommended amount of formalin that can be stored in an area. Does it need to be in a flammable cabinet? The cabinet that we have the formalin in is so old that we are having a vendor to come out to check to see if it suitable for chemical storage. Allison Scott HT(ASCP) Supervisor, Histology Lab LBJ Hospital Harris Health System Office: 713-566-2148 Lab: 713-566-5287 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From RSTAFFORD <@t> mhc.net Fri May 31 12:23:46 2013 From: RSTAFFORD <@t> mhc.net (Stafford, Rosemary) Date: Fri May 31 12:23:55 2013 Subject: [Histonet] RE: Histonet Digest, Vol 114, Issue 30 In-Reply-To: References: Message-ID: <716D642453E3A74899DC169C6274E27D178CC892@MMC-EXCHMBS04.ad.mhc.net> Please remove me from the mailing list. Thank you -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Friday, May 31, 2013 1:02 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 114, Issue 30 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Processing thin slices of mouse cerebellum (Bob Richmond) 2. RE: Extra Money (Mitchell Jean A) 3. HT vs. HTL (McKenzie, Emily) 4. Re: antigen retrieval question (Jan Shivers) 5. Re: Extra Money (Lee & Peggy Wenk) 6. RE: Processing thin slices of mouse cerebellum (Hobbs, Carl) 7. Movats problem solved (Molinari, Betsy) 8. Steiner stain (Herring, Colleen) 9. Autotechnicon processor (margaret chadwell ) 10. Re: Steiner stain (Rene J Buesa) 11. bone marrow aspirations (Diana McCaig) 12. RE: bone marrow aspirations (Hannen, Valerie) 13. Formalin (Scott, Allison D) 14. Re: Formalin (Rene J Buesa) ---------------------------------------------------------------------- Message: 1 Date: Thu, 30 May 2013 13:27:02 -0400 From: Bob Richmond Subject: [Histonet] Re: Processing thin slices of mouse cerebellum To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Tom Truscott replies to Kathleen [Robert]: >>Several years and a couple employers ago we attached small biopsies (for proper orientation) to small squares of cucumber (which had been dehydrated in several changes of alcohol) with a egg-albumin/glycerol mixture. The bx/cucumber unit was embedded together after processing and cut and stained as a unit with no adverse effects.<< I never saw it, but this cucumber technique was in fairly common use among gynecologic pathologists 40 or 50 years ago, for embedding cervical biopsy specimens. The cucumber in alcohol was provided to the GYN performing the biopsy. I think I'd prefer a gin & tonic. Bob Richmond Samurai Pathologist Maryville TN ------------------------------ Message: 2 Date: Thu, 30 May 2013 19:12:04 +0000 From: Mitchell Jean A Subject: RE: [Histonet] Extra Money To: "'Morken, Timothy'" , Sarah Dysart , "histonet@lists.utsouthwestern.edu" Message-ID: <16F90B93CA23D446980B3D591FD02DAD05F4BF@UWHC-MBX14.uwhis.hosp.wisc.edu> Content-Type: text/plain; charset="iso-8859-1" To clarify: Winning NSH poster presentations in both Clinical and VIR categories each receive $250.00 - but NSH does not reimburse any expenses for poster presentation. Hope no one got their hopes up on this.... Jean Mitchell -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Thursday, May 30, 2013 10:53 AM To: joelle weaver; Sarah Dysart; Rene J Buesa; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Extra Money Actually it could be like "earning" money because I believe a poster presenter gets a free trip to the NSH meeting. Workshop presenters certainly do. Why do you think there are so many repeat presenters!?!? Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelle weaver Sent: Thursday, May 30, 2013 8:37 AM To: Sarah Dysart; Rene J Buesa; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Extra Money Maybe to the nice folks at NSH :) Joelle Weaver MAOM, HTL (ASCP) QIHC > From: sdysart@mirnarx.com > To: rjbuesa@yahoo.com; joelleweaver@hotmail.com; > histonet@lists.utsouthwestern.edu > Date: Wed, 29 May 2013 21:05:02 +0000 > Subject: RE: [Histonet] Extra Money > CC: > > I plan on doing a poster for NSH...does that count =) > > Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna > Therapeutics > 2150 Woodward Street > Suite 100 > Austin, Texas 78744 > (512)901-0900 ext. 6912 > > From: Rene J Buesa [mailto:rjbuesa@yahoo.com] > Sent: Wednesday, May 29, 2013 3:37 PM > To: joelle weaver; Sarah Dysart; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Extra Money > > Not even that! If you publish you will build a better c.v. but that is not assurance tof being appreciated by any prospective employer. > In the rare event that it is appreciated you will be able to negotiate a better salary, but that is all! > Ren? J. > > From: joelle weaver > To: Sarah Dysart ; > "histonet@lists.utsouthwestern.edu" > > Sent: Wednesday, May 29, 2013 4:31 PM > Subject: RE: [Histonet] Extra Money > > Publish > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > From: sdysart@mirnarx.com > > To: > > histonet@lists.utsouthwestern.edu > rn.edu> > > Date: Wed, 29 May 2013 20:20:10 +0000 > > Subject: [Histonet] Extra Money > > > > Anyone know any ideas on how to make a couple extra bucks a month using our histology-awesomeness?? > > > > > > Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist > > Mirna Therapeutics > > 2150 Woodward Street > > Suite 100 > > Austin, Texas 78744 > > (512)901-0900 ext. 6912 > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > rn.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu .edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Thu, 30 May 2013 14:59:23 -0500 From: "McKenzie, Emily" Subject: [Histonet] HT vs. HTL To: "'histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain; charset="us-ascii" Can anyone provide job descriptions for HT vs. HTL. We are looking to separated the two positions. If there are any suggestions please let me know. Thank you, Emily McKenzie Memorial Medical Center ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. ------------------------------ Message: 4 Date: Thu, 30 May 2013 15:08:33 -0500 From: Jan Shivers Subject: Re: [Histonet] antigen retrieval question To: "Burton, Lynn" Cc: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Lynn, I use the Decloaker and also stain for BVD, so will get in contact with you later this afternoon. Jan Shivers University of Minnesota Veterinary Diagnostic Lab St. Paul, MN 55108 On Thu, May 30, 2013 at 9:54 AM, Burton, Lynn wrote: > If anyone out there is using a decloaker for antigen retrieval, when > doing ihc staining, would you please contact me on my e-mail address? > I have been struggling with a BVD test for months. It is not working > consistently and I want to explore using this method along with our > Benchmark XT stainer from Ventana. > Thank you, > Lynn Burton > Galesburg Animal Disease Lab > Galesburg, Il > lynn.burton@illinois.gov > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 5 Date: Thu, 30 May 2013 19:28:40 -0400 From: "Lee & Peggy Wenk" Subject: Re: [Histonet] Extra Money To: "Morken, Timothy" , "joelle weaver" , "Sarah Dysart" , "Rene J Buesa" , Message-ID: Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original The poster reimbursement has been addressed. So I'll talk about workshop presenters. It's not a free trip, but some of it is paid. For presenting 1 workshop, you are reimbursed: - air fare or cost of driving, whichever is least - parking at airport (if flying) or at hotel (if driving) - 2 nights of hotel (not to exceed the "convention" hotel rate). You pay for the extra days of hotel you are there. - registration fee ($35), but you have to pay for any workshops you want to attend - 1 banquet ticket - meals for 2 days (I think it's about $35/day). If you know the cost of food at convention hotels, $35 MIGHT cover part of breakfast and lunch. If you are over the $35, you pay for the rest. If you are there more than 2 days, you pay for your own food. So presenting does not give you a free ride to the convention, but it does help reduce the amount that you pay to attend. If anyone is interested in presenting, it works best if you have presented several times at your state meeting/symposium, and start building up a good reputation as a good presenter. NSH usually does not ask a "first time presenter" to talk at a national symposium. If you have no state organization, ask NSH to help you start one. Or, start presenting at in-services where you work. Don't have any of those? Start a monthly in-service, and put that on your resume/CV. (yes, you will need a curriculum vitae to submit to NSH if accepted.) Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: Morken, Timothy Sent: Thursday, May 30, 2013 11:52 AM To: joelle weaver ; Sarah Dysart ; Rene J Buesa ; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Extra Money Actually it could be like "earning" money because I believe a poster presenter gets a free trip to the NSH meeting. Workshop presenters certainly do. Why do you think there are so many repeat presenters!?!? Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelle weaver Sent: Thursday, May 30, 2013 8:37 AM To: Sarah Dysart; Rene J Buesa; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Extra Money Maybe to the nice folks at NSH :) Joelle Weaver MAOM, HTL (ASCP) QIHC > From: sdysart@mirnarx.com > To: rjbuesa@yahoo.com; joelleweaver@hotmail.com; > histonet@lists.utsouthwestern.edu > Date: Wed, 29 May 2013 21:05:02 +0000 > Subject: RE: [Histonet] Extra Money > CC: > > I plan on doing a poster for NSH...does that count =) > > Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna > Therapeutics > 2150 Woodward Street > Suite 100 > Austin, Texas 78744 > (512)901-0900 ext. 6912 > > From: Rene J Buesa [mailto:rjbuesa@yahoo.com] > Sent: Wednesday, May 29, 2013 3:37 PM > To: joelle weaver; Sarah Dysart; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Extra Money > > Not even that! If you publish you will build a better c.v. but that is > not assurance tof being appreciated by any prospective employer. > In the rare event that it is appreciated you will be able to negotiate > a better salary, but that is all! > Ren? J. > > From: joelle weaver > To: Sarah Dysart ; > "histonet@lists.utsouthwestern.edu" > > Sent: Wednesday, May 29, 2013 4:31 PM > Subject: RE: [Histonet] Extra Money > > Publish > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > From: sdysart@mirnarx.com > > To: > > histonet@lists.utsouthwestern.edu > rn.edu> > > Date: Wed, 29 May 2013 20:20:10 +0000 > > Subject: [Histonet] Extra Money > > > > Anyone know any ideas on how to make a couple extra bucks a month > > using our histology-awesomeness?? > > > > > > Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist > > Mirna Therapeutics > > 2150 Woodward Street > > Suite 100 > > Austin, Texas 78744 > > (512)901-0900 ext. 6912 > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > rn.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu .edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Fri, 31 May 2013 07:30:45 +0000 From: "Hobbs, Carl" Subject: RE: [Histonet] Processing thin slices of mouse cerebellum To: "histonet@lists.utsouthwestern.edu" Message-ID: <00D6B8253EAED840B8D04E235497381831FF23AD@AM2PRD0311MB399.eurprd03.prod.outlook.com> Content-Type: text/plain; charset="iso-8859-1" http://www.cellpath.us.com/magento/cellpath-on-line-shop/specimen-processing-and-embedding/small-biospy-processing/cellsafe/cellsafe-biopsy-capsule-blue.html I find them very good for brain slices. There is a deeper and a shallower space, depending on which way they are folded. Carl Hobbs FIBMS Histology and Imaging Manager Wolfson Centre for Age-Related Diseases School of Biomedical Sciences King's College London Guys Campus London SE1 1UL ? Tel.020 7848 6810 fax 020 7848 6816 carl.hobbs@kcl.ac.uk ------------------------------ Message: 7 Date: Fri, 31 May 2013 11:00:07 +0000 From: "Molinari, Betsy" Subject: [Histonet] Movats problem solved To: "Histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="utf-8" Good Friday to all. Histology 101. The Alcian Blue dye itself had lost its efficacy. I borrowed a solution from another lab and voila, perfect staining. Thank you to everyone for their suggestions. I will certainly keep them on file in the event this ever happens again. Have a good weekend. Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 1101 Bates St. Houston,TX 77030 832-355-6524 (lab) 832-355-6812 (fax) Betsy Molinari Senior Histology Research Technician 832-355-6524 | BMolinari@texasheart.org | www.texasheart.org 6770 Bertner Ave., MC 1-283, Houston, TX 77030 [Texas Heart Institute][THI News] [THI on Facebook] [THI on Flicker] [THI on Google] [THI on Pinterest] [THI on Twitter] [THI on You Tube] Confidentiality Notice: This message may be confidential and/or privileged. If you are not the intended recipient you may not review, disseminate or copy this e-mail, its contents and/or any attachments. Please immediately notify the sender If you have received this e-mail in error and delete it from your computer system. ------------------------------ Message: 8 Date: Fri, 31 May 2013 07:16:49 -0400 From: "Herring, Colleen" Subject: [Histonet] Steiner stain To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" I am looking for some suggestions on a problem with our steiner stain. We have a percipitate on the whole slide not just on the tissue and no matter what we have done I can't seem to get rid of it. HELP! ________________________________________________________________________________________________________ The information in this communication is intended to be confidential to the Individual(s) and/or Entity to whom it is addressed. It may contain information of a Privileged and/or Confidential nature, which is subject to Federal and/or State privacy regulations. In the event that you are not the intended recipient or the agent of the intended recipient, do not copy or use the information contained within this communication, or allow it to be read, copied or utilized in any manner, by any other person(s). Should this communication be received in error, please notify the sender immediately either by response e-mail or by phone, and permanently delete the original e-mail, attachment(s), and any copies. ________________________________________________________________________________________________________ ------------------------------ Message: 9 Date: Fri, 31 May 2013 11:27:58 +0000 From: "margaret chadwell " Subject: [Histonet] Autotechnicon processor To: "histonet@lists.utsouthwestern.edu " Message-ID: Content-Type: text/plain; charset="iso-8859-15" Does anyone have an Autotechnicon Tissue Processor for sale?Thanks much!Margie Chadwell ------------------------------ Message: 10 Date: Fri, 31 May 2013 07:11:29 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Steiner stain To: "Herring, Colleen" , "Histonet@lists.utsouthwestern.edu" Message-ID: <1370009489.5987.YahooMailNeo@web163106.mail.bf1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Usually any (and almost all) precipitates when using a silver stain is produced by the silver solution reacting over some remnant of a previous solution not completely removed. Check your solutions, if needed prepare them anew and try again. Ren? J. From: "Herring, Colleen" To: Histonet@lists.utsouthwestern.edu Sent: Friday, May 31, 2013 7:16 AM Subject: [Histonet] Steiner stain I am looking for some suggestions on a problem with our steiner stain. We have a percipitate on the whole slide not just on the tissue and no matter what we have done I can't seem to get rid of it. HELP! ________________________________________________________________________________________________________ The information in this communication is intended to be confidential to the Individual(s) and/or Entity to whom it is addressed. It may contain information of a Privileged and/or Confidential nature, which is subject to Federal and/or State privacy regulations. In the event that you are not the intended recipient or the agent of the intended recipient, do not copy or use the information contained within this communication, or allow it to be read, copied or utilized in any manner, by any other person(s). Should this communication be received in error, please notify the sender immediately either by response e-mail or by phone, and permanently delete the original e-mail, attachment(s), and any copies. ________________________________________________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Fri, 31 May 2013 15:00:57 +0000 From: Diana McCaig Subject: [Histonet] bone marrow aspirations To: "'histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain; charset="us-ascii" Can anyone share with me their process for processing bone marrow aspirations and embedding them in paraffin blocks. Currently ours are being spread out throughout the entire base mold and makes it cumbersome to screen. Has anyone used histogel or making a button and embed that instead of just filtering the aspirate and putting that is a cassette. Thanks Diana ------------------------------ Message: 12 Date: Fri, 31 May 2013 11:49:21 -0400 From: "Hannen, Valerie" Subject: [Histonet] RE: bone marrow aspirations To: 'Diana McCaig' , "'histonet@lists.utsouthwestern.edu'" Message-ID: <450B7A81EDA0C54E97C53D60F00776C3232B4BB5E9@isexstore03> Content-Type: text/plain; charset="us-ascii" Diana, We let the aspirates clot in a petri dish, cover them in formalin to fix the clot and then make a block from the fixed clot. Process overnight in the processor and embed the clot in the A.M. This process helps eliminate the aspirate from spreading out in the base mold. I hope this helps. Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.hannen@parrishmed.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Diana McCaig Sent: Friday, May 31, 2013 11:01 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] bone marrow aspirations Can anyone share with me their process for processing bone marrow aspirations and embedding them in paraffin blocks. Currently ours are being spread out throughout the entire base mold and makes it cumbersome to screen. Has anyone used histogel or making a button and embed that instead of just filtering the aspirate and putting that is a cassette. Thanks Diana _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet =================== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" =================== ------------------------------ Message: 13 Date: Fri, 31 May 2013 16:31:05 +0000 From: "Scott, Allison D" Subject: [Histonet] Formalin To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Hello to all in histoland. Happy Friday. Last week we were inspected by a new group of surveyors that are like JCAHO called DNV. They said that we had to much formalin stored in our gross room. We had 15 gallons. They told us that we could only have 2 gallons there at a time. I have never heard of such. We were just inspected by CAP and I might add that it was an intense inspection. They checked everything. This never came up. What is the recommended amount of formalin that can be stored in an area. Does it need to be in a flammable cabinet? The cabinet that we have the formalin in is so old that we are having a vendor to come out to check to see if it suitable for chemical storage. Allison Scott HT(ASCP) Supervisor, Histology Lab LBJ Hospital Harris Health System Office: 713-566-2148 Lab: 713-566-5287 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. ------------------------------ Message: 14 Date: Fri, 31 May 2013 09:41:17 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Formalin To: "Scott, Allison D" , "histonet@lists.utsouthwestern.edu" Message-ID: <1370018477.61740.YahooMailNeo@web163103.mail.bf1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Formalin is THEORETICALLY (and very UNLIKELY) "flammable, but putting it into a flammables cabinet is going too far. Try to find out those conflicting standards (JCAHO vs. CAP) and follow the strictest. Also please remember that many inspectors just "love to show off!" Some think that have to "cite for something" because otherwise they are not doing a good job, or do not know enough.? Ren? J. From: "Scott, Allison D" To: "histonet@lists.utsouthwestern.edu" Sent: Friday, May 31, 2013 12:31 PM Subject: [Histonet] Formalin Hello to all in histoland.? Happy Friday.? Last week we were inspected by a new group of surveyors that are like JCAHO called DNV.? They said that we had to much formalin stored in our gross room.? We had 15 gallons.? They told us that we could only have 2 gallons there at a time.? I have never heard of such.? We were just inspected by CAP and I might add that it was an? intense inspection.? They checked everything. This never came up.? What is the recommended amount of formalin that can be stored in an area.? Does it need to be in a flammable cabinet?? The cabinet that we have the formalin in is so old that we are? having a vendor to come out to check to see if it suitable for chemical storage. Allison Scott HT(ASCP) Supervisor, Histology Lab LBJ Hospital Harris Health System Office: 713-566-2148 Lab: 713-566-5287 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged.? This e-mail may also be confidential and/or privileged under Texas law.? The e-mail is for the use of only the individual or entity named above.? If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 114, Issue 30 ***************************************** From darkdaym <@t> comcast.net Fri May 31 13:26:53 2013 From: darkdaym <@t> comcast.net (darkdaym@comcast.net) Date: Fri May 31 13:27:16 2013 Subject: [Histonet] RE: Formalin In-Reply-To: <14E2C6176416974295479C64A11CB9AE016411AC2EEF@SBS2K8.premierlab.local> References: <14E2C6176416974295479C64A11CB9AE016411AC2EEF@SBS2K8.premierlab.local> Message-ID: <51A8EB6D.5020404@comcast.net> Elizabethand Allison, The US adopted the Globally Harmonized System(GHS) some time ago. Compliance is not yet required, but you might want to check out 29CFR1910.1200. It will probably affect all labs to some extent. Just enter that term in your browser. The OSHA GHS storage requirement for Flammable Liquids is at 29CFR1910.1200 AppendixC.4.1.9. Formalin Fixative falls in Category 4. "Store in a well-ventilated place. Keep cool."That's all. No limits of any kind are mentionedunder the new standards. Of course, any workplace maybe subject to more stringent regulations. Regards, Mark On 5/31/2013 12:01 PM, Elizabeth Chlipala wrote: > Allison > > I have not heard of that either, that would mean that you could not even store one 5 gallon cube of formalin. I would politely ask them for a copy of the regulation that states that there can only be 2 gallons of formalin stored in your gross room. They may be classifying the formalin as a flammable, which I think may be happening in the future once the US adopts "Global Standards" but I don't believe we are there right now. We keep at most 10 gallons of formalin in the lab. One 5 gallon cube that is opened and then a second cube that has not been opened. NSH had a really nice teleconference recently on Training for Revised Hazard Communication Standard by Ada Feldman. That teleconference was from March 27, 2013. I'm not certain but I believe that if the US adopts these so called "global standards" that formalin would be classified as a flammable as well as quite of few of the other chemicals we use in the histology laboratory. Maybe there is someone out on the Histonet that knows a bit more than me that can comment on this. > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > Laboratory Manager > Premier Laboratory, LLC > PO Box 18592 > Boulder, CO 80308 > Work (303) 682-3949 > Fax (303) 682-9060 > Cell (303) 881-0763 > liz@premierlab.com > www.premierlab.com > > Ship to address: > > 1567 Skyway Drive, Unit E > Longmont, CO 80504 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D > Sent: Friday, May 31, 2013 10:31 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Formalin > > Hello to all in histoland. Happy Friday. Last week we were inspected by a new group of surveyors that are like JCAHO called DNV. They said that we had to much formalin stored in our gross room. We had 15 gallons. They told us that we could only have 2 gallons there at a time. I have never heard of such. We were just inspected by CAP and I might add that it was an intense inspection. They checked everything. This never came up. What is the recommended amount of formalin that can be stored in an area. Does it need to be in a flammable cabinet? The cabinet that we have the formalin in is so old that we are having a vendor to come out to check to see if it suitable for chemical storage. > > Allison Scott HT(ASCP) > Supervisor, Histology Lab > LBJ Hospital > Harris Health System > Office: 713-566-2148 > Lab: 713-566-5287 > > > CONFIDENTIALITY NOTICE: > If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. > > To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From BSullivan <@t> virtua.org Fri May 31 13:39:52 2013 From: BSullivan <@t> virtua.org (Sullivan, Beatrice) Date: Fri May 31 13:39:58 2013 Subject: [Histonet] The use of PA's Message-ID: <6932520047F7EE46B512E9801344F16032309C92@ExchangeMB-1.Virtua.org> To anyone out there utilizing PA's in their histology lab. I am in the early phase of establishing a realistic benchmark for case load per PA. We are a multidivisional facility. Currently there are 3 fulltime PA's. We are not open on weekends or holidays. They do not do autopsies. They do most frozens. This amount is not an overwhelming number. Majority of the biopsies are handled by trained histo-techs. We are not a teaching facility. We have a standard case mix. Any suggestions? Beatrice L. Sullivan HT(ASCP)HTL Corporate Histology Manager Virtua, Voorhees 856-247-3144 This message, and any included attachments, are from Virtua Health or its related affiliates and is intended only for the addressee(s). The information contained herein is privileged, proprietary or may include confidential information and/or protected patient health information. Any unauthorized review, forwarding, printing, copying, distributing, or otherwise disseminating or taking any action based on such information is strictly prohibited. If you have received this message in error, or have reason to believe you are not authorized to receive it, please delete this message promptly and notify the sender by e-mail with a copy to ISSECURITY@virtua.org. Thank you From mturner <@t> CSILaboratories.com Fri May 31 13:44:00 2013 From: mturner <@t> CSILaboratories.com (Mark Turner) Date: Fri May 31 13:44:24 2013 Subject: [Histonet] RE: Formalin In-Reply-To: <51A8EB6D.5020404@comcast.net> References: <14E2C6176416974295479C64A11CB9AE016411AC2EEF@SBS2K8.premierlab.local> <51A8EB6D.5020404@comcast.net> Message-ID: <643626B74DE2814D8537057F40E1A10B044763BF@CSI-MX-NODEA.CSI-LABS.local> I don't believe there are any regulations on the national or federal level, however you might want to check any local regulations pertaining to storage amounts. I have found these to vary widely, many times being much more strict than national regulations. A 2 gallon maximum is not realistic in either case. Mark Turner, HT(ASCP)QIHC Manager, Histology/IHC ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of darkdaym@comcast.net Sent: Friday, May 31, 2013 2:27 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Formalin Elizabethand Allison, The US adopted the Globally Harmonized System(GHS) some time ago. Compliance is not yet required, but you might want to check out 29CFR1910.1200. It will probably affect all labs to some extent. Just enter that term in your browser. The OSHA GHS storage requirement for Flammable Liquids is at 29CFR1910.1200 AppendixC.4.1.9. Formalin Fixative falls in Category 4. "Store in a well-ventilated place. Keep cool."That's all. No limits of any kind are mentionedunder the new standards. Of course, any workplace maybe subject to more stringent regulations. Regards, Mark On 5/31/2013 12:01 PM, Elizabeth Chlipala wrote: > Allison > > I have not heard of that either, that would mean that you could not even store one 5 gallon cube of formalin. I would politely ask them for a copy of the regulation that states that there can only be 2 gallons of formalin stored in your gross room. They may be classifying the formalin as a flammable, which I think may be happening in the future once the US adopts "Global Standards" but I don't believe we are there right now. We keep at most 10 gallons of formalin in the lab. One 5 gallon cube that is opened and then a second cube that has not been opened. NSH had a really nice teleconference recently on Training for Revised Hazard Communication Standard by Ada Feldman. That teleconference was from March 27, 2013. I'm not certain but I believe that if the US adopts these so called "global standards" that formalin would be classified as a flammable as well as quite of few of the other chemicals we use in the histology laboratory. Maybe there is someone out on the Histonet that knows a bit more than me that can comment on this. > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Laboratory Manager Premier > Laboratory, LLC PO Box 18592 Boulder, CO 80308 Work (303) 682-3949 Fax > (303) 682-9060 Cell (303) 881-0763 liz@premierlab.com > www.premierlab.com > > Ship to address: > > 1567 Skyway Drive, Unit E > Longmont, CO 80504 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott, > Allison D > Sent: Friday, May 31, 2013 10:31 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Formalin > > Hello to all in histoland. Happy Friday. Last week we were inspected by a new group of surveyors that are like JCAHO called DNV. They said that we had to much formalin stored in our gross room. We had 15 gallons. They told us that we could only have 2 gallons there at a time. I have never heard of such. We were just inspected by CAP and I might add that it was an intense inspection. They checked everything. This never came up. What is the recommended amount of formalin that can be stored in an area. Does it need to be in a flammable cabinet? The cabinet that we have the formalin in is so old that we are having a vendor to come out to check to see if it suitable for chemical storage. > > Allison Scott HT(ASCP) > Supervisor, Histology Lab > LBJ Hospital > Harris Health System > Office: 713-566-2148 > Lab: 713-566-5287 > > > CONFIDENTIALITY NOTICE: > If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. > > To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MSHERWOOD <@t> PARTNERS.ORG Fri May 31 14:16:45 2013 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret) Date: Fri May 31 14:16:49 2013 Subject: [Histonet] Re: Inexpensive microwave for staining Message-ID: <16F356143B1CE2459BC129BF68AD0F0F14E39C70@PHSX10MB25.partners.org> To all: I realize that this question has come up before, but what microwave do most labs recommend for special staining? We have never had the need for one, but now do. We would like an inexpensive one. Thanks! Peggy Peggy Sherwood Research Specialist, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From carl.hobbs <@t> kcl.ac.uk Fri May 31 14:19:40 2013 From: carl.hobbs <@t> kcl.ac.uk (Hobbs, Carl) Date: Fri May 31 14:19:54 2013 Subject: [Histonet] CD45 Ab Message-ID: <00D6B8253EAED840B8D04E235497381831FF24B5@AM2PRD0311MB399.eurprd03.prod.outlook.com> I need an Ab against CD45 ( LCA) that works in mouse FFPWS. I would be most grateful for a solid recommendation ( you know it works, rather than what is said on spec sheet) Thank you Carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge Kings College London London SE1 1UL 020 7848 6813 From christen <@t> vet.k-state.edu Fri May 31 14:37:29 2013 From: christen <@t> vet.k-state.edu (Shelly Christenson) Date: Fri May 31 14:37:38 2013 Subject: [Histonet] Histology Lab Manager postion openig Message-ID: <06E342B6098ED9478347E1407764C80407D2BC88@VETMXHT.ads.vet.k-state.edu> We are currently looking for a Histology Lab Manager at the Veterinary Diagnostic Lab, Kansas State University in Manhattan Kansas. If interested in the position I have listed the advertisement information below or if you want more detailed information on the position you can contact Dr. Jamie Henningson at: heningsn@vet.k-state.edu 785/532-4129 Veterinary Diagnostic Laboratory - Manager Position A full-time Laboratory Manager position is available in the Kansas State Veterinary Diagnostic Laboratory in the College of Veterinary Medicine at Kansas State University. This position is responsible for managing the histopathology/immunohistochemistry laboratory and coordinating work responsibilities within the lab. This position supervises, oversees, and directs the lab to coordinate technologies within the lab and facilitate efficient daily operations. A master's degree is required or a bachelor's degree in a science-related field such as chemistry, biochemistry, biological sciences, zoology or microbiology plus a minimum of 5 years of histopathology laboratory experience can be substitute for a master's degree. Previous supervision of personnel is preferred. This is a full-time appointment with benefits. Salary is commensurate with experience. Interested applicants should send a cover letter, resume and contact information for three professional references to Brooke Stallbaumer, 102 Trotter Hall, Manhattan, KS 66506 or bstall@vet.ksu.edu. Screening of applications will begin 6/24/2013 and continue until a suitable candidate is identified. KSU is an AA/EOE. Background check required. Shelly Christenson HT (ASCP) Veterinary Diagnostic Lab Histopathology Mosier Hall L-216 Kansas State University 1800 Denison Manhattan, KS 66506 785/532-4464 christen@vet.k-state.edu