[Histonet] Lymph node retrieval solution

[Douglas Porter]

We use a 50/50 solution of Acetone and Alcohol.  It renders the fat somewhat
transparent and turns the lymph nodes white.  Keep in mind you need to fix
the specimen properly BEFORE you use this technique.  You will also need to
use 3 to 4 changes at about 15 minutes each.  Separate, pull apart, the
bigger pieces to get better penetration of the solution and stir after first
five minutes or so.  I routinely find 1mm lymph nodes this way.  Happy
hunting!

Douglas A. Porter, HT (ASCP) 
Grossing Technician 
IT Coordinator
Cancer Registrar 

CAP-Lab, PLC 
2508 South Cedar Street 			
Lansing, MI 48910-3138 

517-372-5520 (phone) 
517-372-5540 (fax) 

doug.porter <@t> caplab.org 

www.caplab.org 		
 
 
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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Truscott,
Tom
Sent: Friday, March 29, 2013 10:33 AM
To: Bernadette del Rosario; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Lymphnode retrieval solution

Hi Bernadette, Have you tried soaking the tissue in white vinegar? Tom
Truscott

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Bernadette
del Rosario
Sent: Friday, March 29, 2013 3:22 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Lymphnode retrieval solution

Has anyone there can share the best solution for retrieval of lymphnodes in
the fats.Other than the GEWF/Penfix solutions,has anyone used a xylene based
solution which will dissolved the fats making it transparent looking and for
lymphnodes to be seen easily?Cant find anything in the google..Highly
appreciate any methods.
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