[Histonet] Immunofluorescence on FFPE skin

[Martin, Erin]

Hello all,



My pathologist gave me a copy of "Immunofluorescence with Dual Microwave Retrieval of Paraffin-Embedded Sections in the Assessment of Human Renal Biopsy Specimens" from the American Journal of Clinical Pathology.  He said that the same principle should work on skin and he would like to be able to do IF on fixed tissue in addition to our usual cryostat sections.  Has anyone else read the paper who might be willing to give me some basic advice to try working it out?  Is a microwave necessary (paper's method uses 2 different wattage settings) or is there a way to use HIER in waterbath or pressure cooker?



Thanks in advance,

Erin



Erin Martin, Histology Supervisor
UCSF  Dermatopathology Service
415-353-7248

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