From vikk_0521 <@t> yahoo.com Fri Mar 1 02:31:11 2013 From: vikk_0521 <@t> yahoo.com (Vikrant Piprode) Date: Fri Mar 1 02:31:32 2013 Subject: [Histonet] (no subject) Message-ID: <1362126671.40509.YahooMailNeo@web162904.mail.bf1.yahoo.com> hello, I have tried staining my calvarial bone sections for Tartrate Resistant Acid Phosphatase but it didnt work out. Can u help me out in this regard. Thank you? Vikrant Piprode From histotalk <@t> yahoo.com Fri Mar 1 09:42:31 2013 From: histotalk <@t> yahoo.com (David Kemler) Date: Fri Mar 1 09:42:36 2013 Subject: [Histonet] The GSH 4oth Anniversary Meeting Message-ID: <1362152551.97608.YahooMailNeo@web121501.mail.ne1.yahoo.com> Hi Folks - Just a quick reminder that HistoTALK http://www.histotalk.com/ has been invited by the GSH board to participate in the festivities again this year! The GSH meeting is always a great opportunity to help fulfill your CEU requirements attending workshops?by the very best speakers and presenters in the histology field! ? I'm not presenting this year at the GSH, but I will be there with the HistoTALK "mobile" studio for guest interviews. ? Jekyll Island is a fantastic place for a state meeting and a great opportunity to mix and mingle. Be sure to join us for a way-cool time! GSH members are FUN folks! I know once you go to just one of their state?meetings - you'll be hooked! ? The date is April 12th - 14th, so there's still time to register. ? And again, thanks GSH board for inviting me and HistoTALK to?be part of?your 40th Anniversary Celebration! ? Yours, David http://www.histotalk.com/ From rhworkman <@t> uro.com Fri Mar 1 10:26:19 2013 From: rhworkman <@t> uro.com (Renee H. Workman) Date: Fri Mar 1 10:26:32 2013 Subject: [Histonet] QIHC Message-ID: I will be taking the QIHC soon has anyone taken it recently. I lost my QIHC when I changed jobs and want to get it back. Renee H. Workman W: 804-527-1316 | F: 804-270-0917 rhworkman@uro.com | www.uro.com Disclaimer: The email and files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the original recipient or the person responsible for the delivering the email to the intended recipient, be advised that you have received this email in error, and that any use, dissemination, forwarding, printing or copying of this email is strictly prohibited. If you received this email in error, please delete it from your system without copying it, and notify the sender by reply email so that our address record can be corrected. From BDeBrosse-Serra <@t> isisph.com Fri Mar 1 12:05:26 2013 From: BDeBrosse-Serra <@t> isisph.com (Bea DeBrosse-Serra) Date: Fri Mar 1 12:05:40 2013 Subject: [Histonet] Test Message-ID: <493CAA64F203E14E8823737B9EE0E25F092E5BA9DD@EXCHMB01.isis.local> From joelleweaver <@t> hotmail.com Fri Mar 1 12:33:25 2013 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Fri Mar 1 12:33:28 2013 Subject: [Histonet] QIHC In-Reply-To: References: Message-ID: Yes, I took it pretty recently. It is pretty straightforward. I just reviewed the Dako handbook. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: rhworkman@uro.com > To: histonet@lists.utsouthwestern.edu > Date: Fri, 1 Mar 2013 16:26:19 +0000 > Subject: [Histonet] QIHC > > I will be taking the QIHC soon has anyone taken it recently. I lost my QIHC when I changed jobs and want to get > > it back. > > > Renee H. Workman > W: 804-527-1316 | F: 804-270-0917 > rhworkman@uro.com | www.uro.com > > > > > > Disclaimer: The email and files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the original recipient or the person responsible for the delivering the email to the intended recipient, be advised that you have received this email in error, and that any use, dissemination, forwarding, printing or copying of this email is strictly prohibited. If you received this email in error, please delete it from your system without copying it, and notify the sender by reply email so that our address record can be corrected. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From abdulrahmanmsaadalla <@t> gmail.com Fri Mar 1 12:41:21 2013 From: abdulrahmanmsaadalla <@t> gmail.com (abdulrahman saadalla) Date: Fri Mar 1 12:54:38 2013 Subject: [Histonet] Fixed cells dried out! Message-ID: I fixed some cells in matrigel and left them in PBS for a week, and today I found some of the 4 out of the 8 wells dry! Is this really going to affect staining? Thanks From jennifer.masuzumi <@t> sterlingpath.com Fri Mar 1 13:09:03 2013 From: jennifer.masuzumi <@t> sterlingpath.com (Jennifer Masuzumi) Date: Fri Mar 1 13:09:06 2013 Subject: [Histonet] Ventana HPV High/Low Replacements? Message-ID: <1362164943.49368.YahooMailNeo@web5814.biz.mail.ne1.yahoo.com> Hello everyone, My lab is interested in starting?HPV?High and Low Risk ISH testing, but it seems like we just missed the boat on it. We have Benchmark XTs, but Ventana discontinued their HPV probes sometime around last December, and they don't have an alternative at this time. After talking with nearby labs that are currently using stockpiles of Ventana probes, they told me the following courses of action they were planning on using when their stock runs out: -One lab is thinking of doing HPV by PCR -Another is thinking of switching to Dako probes After discussing the possibility of Dako probes with Ventana and Dako, neither could recommend using them together because each side was unfamiliar with the other's reagents or system, though both said it might be possible. Is anybody in a similar situation, looking for a replacement for the Ventana HPV High/Low Risk probes? Any insight on possibilities to pursue would be much appreciated! Sincerely, ? Jennifer Masuzumi Sterling Pathology Seal Beach, CA jennifer.masuzumi@sterlingpath.com 562-799-8900 From akbitting <@t> geisinger.edu Fri Mar 1 13:59:17 2013 From: akbitting <@t> geisinger.edu (Bitting, Angela K.) Date: Fri Mar 1 13:59:33 2013 Subject: [Histonet] Ventana HPV High/Low Replacements? In-Reply-To: <1362164943.49368.YahooMailNeo@web5814.biz.mail.ne1.yahoo.com> References: <1362164943.49368.YahooMailNeo@web5814.biz.mail.ne1.yahoo.com> Message-ID: <77F52EFAB8B1694B885E277C48FCD0F632851447@GHSEXMBX4W8K1V.geisinger.edu> Just spoke with my Ventana rep about this same topic. My understanding is ANY vendor who sells HPV probe for ISH is out of compliance with FDA and is eventually going to get in trouble. I specifically asked if I could buy probe from elsewhere and was told "not likely". This is only a problem for us here in the US because of FDAs restrictions. I hope someone will tell me I misunderstood. Ventana suggests running p16 if you don't already. Just throwing in my 2 cents. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer Masuzumi Sent: Friday, March 01, 2013 2:09 PM To: Histonet Subject: [Histonet] Ventana HPV High/Low Replacements? Hello everyone, My lab is interested in starting?HPV?High and Low Risk ISH testing, but it seems like we just missed the boat on it. We have Benchmark XTs, but Ventana discontinued their HPV probes sometime around last December, and they don't have an alternative at this time. After talking with nearby labs that are currently using stockpiles of Ventana probes, they told me the following courses of action they were planning on using when their stock runs out: -One lab is thinking of doing HPV by PCR -Another is thinking of switching to Dako probes After discussing the possibility of Dako probes with Ventana and Dako, neither could recommend using them together because each side was unfamiliar with the other's reagents or system, though both said it might be possible. Is anybody in a similar situation, looking for a replacement for the Ventana HPV High/Low Risk probes? Any insight on possibilities to pursue would be much appreciated! Sincerely, ? Jennifer Masuzumi Sterling Pathology Seal Beach, CA jennifer.masuzumi@sterlingpath.com 562-799-8900 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. ------------------------------------------------------------------------- This message was secured by ZixCorp(R). From rheyna <@t> lumc.edu Fri Mar 1 17:14:01 2013 From: rheyna <@t> lumc.edu (Roger Heyna) Date: Fri Mar 1 17:14:13 2013 Subject: [Histonet] Ubiquitin Primary Antibody for IHC Message-ID: <5130E1D9020000230004B140@gwgwia1.luhs.org> Our lab would like to routinely run the Ubiquitin IHC stain on formalin-fixed, paraffin-embedded brain and liver(mallory bodies), and I was wondering if anyone could recommend a primary antibody. We are currently using the Ventana Benchmark XT autostainer. Thank you, Roger Heyna Maywood, IL From turkekul <@t> gmail.com Fri Mar 1 18:40:54 2013 From: turkekul <@t> gmail.com (mesruh turkekul) Date: Fri Mar 1 18:40:59 2013 Subject: [Histonet] Re: Histonet Digest, Vol 112, Issue 1 In-Reply-To: <5130ecde.422fb60a.4764.044eSMTPIN_ADDED_MISSING@mx.google.com> References: <5130ecde.422fb60a.4764.044eSMTPIN_ADDED_MISSING@mx.google.com> Message-ID: Dear Histonetters, I am studying bone and teeth growth in rat maxilla. I will inject calcein green and would like to fix, embed and sections the rat maxilla. Any suggestions for the best method to fix, embed and section the samples for fluorescnet microscopy? Thank you very much! Mes HTL (ASCP) Memorial Sloan-Kettering Cancer Center On Fri, Mar 1, 2013 at 1:01 PM, wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. FNA Clia Guidelines (PicheGrocki, Jessica) > 2. RE: FNA Clia Guidelines (Horn, Hazel V) > 3. GSH Symposium April 12-14 (Zimmerman, Billie) > 4. FSH abstracts deadline (Jerry Santiago, MSEd, HTL (ASCP) QIHC) > 5. (no subject) (Vikrant Piprode) > 6. The GSH 4oth Anniversary Meeting (David Kemler) > 7. QIHC (Renee H. Workman) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Thu, 28 Feb 2013 20:26:30 +0000 > From: "PicheGrocki, Jessica" > Subject: [Histonet] FNA Clia Guidelines > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > < > 631955447A364B45B9458D2905635110655D26B9@WIN08-MBX-01.wtbyhosp.org> > Content-Type: text/plain; charset="us-ascii" > > Hi All, > > Quick question............what are the Clia requirements for Fine needle > aspirate procedures? Is it considered high complexity testing? And who > prepares the slides when the needle is handed off? > > Thank you, > > Jessica Piche, HT(ASCP) > > > > CONFIDENTIALITY NOTICE: This email and any attachments contain > confidential information that is legally privileged. This information is > intended only for the use of the individual or entity named above. The > authorized recipient of this information is prohibited from disclosing this > information to any other party unless required to do so by law or > regulation. If you are not the intended recipient, you are hereby notified > that any disclosure, copying, distribution or action taken in reliance on > the contents of these documents is strictly prohibited. If you have > received this information in error, please notify the sender immediately > and delete these documents. Copyright (c) Waterbury Hospital > > > ------------------------------ > > Message: 2 > Date: Thu, 28 Feb 2013 15:05:35 -0600 > From: "Horn, Hazel V" > Subject: [Histonet] RE: FNA Clia Guidelines > To: "'PicheGrocki, Jessica'" , > "histonet@lists.utsouthwestern.edu" > > Message-ID: > <25A4DE08332B19499904459F00AAACB719BE13564F@EVS1.archildrens.org> > Content-Type: text/plain; charset="us-ascii" > > Our pathologists do it all. > > Hazel Horn > Supervisor of Histology/Autopsy/Transcription > Anatomic Pathology > Arkansas Children's Hospital > 1 Children's Way | Slot 820| Little Rock, AR 72202 > 501.364.4240 direct | 501.364.1241 fax > hornhv@archildrens.org > archildrens.org > > > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of PicheGrocki, > Jessica > Sent: Thursday, February 28, 2013 2:27 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] FNA Clia Guidelines > > Hi All, > > Quick question............what are the Clia requirements for Fine needle > aspirate procedures? Is it considered high complexity testing? And who > prepares the slides when the needle is handed off? > > Thank you, > > Jessica Piche, HT(ASCP) > > > > CONFIDENTIALITY NOTICE: This email and any attachments contain > confidential information that is legally privileged. This information is > intended only for the use of the individual or entity named above. The > authorized recipient of this information is prohibited from disclosing this > information to any other party unless required to do so by law or > regulation. If you are not the intended recipient, you are hereby notified > that any disclosure, copying, distribution or action taken in reliance on > the contents of these documents is strictly prohibited. If you have > received this information in error, please notify the sender immediately > and delete these documents. Copyright (c) Waterbury Hospital > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** > The information contained in this message may be privileged and > confidential > and protected from disclosure. If the reader of this message is not the > intended recipient, or an employee or agent responsible for delivering this > message to the intended recipient, you are hereby notified that any > dissemination, distribution or copying of this communication is strictly > prohibited. If you have received this communication in error, please notify > us immediately by replying to the message and deleting it from your > computer. > Thank you. > > > > ------------------------------ > > Message: 3 > Date: Thu, 28 Feb 2013 21:41:34 +0000 > From: "Zimmerman, Billie" > Subject: [Histonet] GSH Symposium April 12-14 > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > < > 7B3DEB32E69C034EACB479059C5DE3FF758BF9@EX-MLB-03.ad.georgiahealth.edu> > > Content-Type: text/plain; charset="us-ascii" > > Don't forget to register for the Georgia Society of Histotechnology > meeting April 12-14. > What a great getaway weekend to earn up to 15 CEU's from the NSH. Join us > for education, > networking, and some great scenery! > > Wanda Simons > President of GSH > > /bz > Augusta State University and Georgia Health Sciences University have > consolidated to become Georgia Regents University. Effective January 9, > 2013, my email address has changed to BZIMMERM@gru.edu. Please update > your address book to reflect this change. > > > ------------------------------ > > Message: 4 > Date: Thu, 28 Feb 2013 20:51:06 -0500 > From: "Jerry Santiago, MSEd, HTL (ASCP) QIHC" > > Subject: [Histonet] FSH abstracts deadline > To: histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; charset=utf-8 > > > Dear histonetters, > > March 1st is the ddeadline to submit any abstracts consideration for the > 2013 Florida Society for Histotechnology meeting. To submit an abstract go > to www.fshgroup.org > > Or email to fsh@fshgroup.org > > Jerry Santiago > FSH > > ------------------------------ > > Message: 5 > Date: Fri, 1 Mar 2013 00:31:11 -0800 (PST) > From: Vikrant Piprode > Subject: [Histonet] (no subject) > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > <1362126671.40509.YahooMailNeo@web162904.mail.bf1.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > hello, > I have tried staining my calvarial bone sections for Tartrate Resistant > Acid Phosphatase but it didnt work out. Can u help me out in this regard. > > Thank you > Vikrant Piprode > > ------------------------------ > > Message: 6 > Date: Fri, 1 Mar 2013 07:42:31 -0800 (PST) > From: David Kemler > Subject: [Histonet] The GSH 4oth Anniversary Meeting > To: Fellow HistoNetters > Message-ID: > <1362152551.97608.YahooMailNeo@web121501.mail.ne1.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Hi Folks - Just a quick reminder that HistoTALK http://www.histotalk.com/has been invited by the GSH board to participate in the festivities again > this year! The GSH meeting is always a great opportunity to help fulfill > your CEU requirements attending workshops by the very best speakers and > presenters in the histology field! > > I'm not presenting this year at the GSH, but I will be there with the > HistoTALK "mobile" studio for guest interviews. > > Jekyll Island is a fantastic place for a state meeting and a great > opportunity to mix and mingle. Be sure to join us for a way-cool time! GSH > members are FUN folks! I know once you go to just one of their > state meetings - you'll be hooked! > > The date is April 12th - 14th, so there's still time to register. > > And again, thanks GSH board for inviting me and HistoTALK to be part > of your 40th Anniversary Celebration! > > Yours, > David > http://www.histotalk.com/ > > ------------------------------ > > Message: 7 > Date: Fri, 1 Mar 2013 16:26:19 +0000 > From: "Renee H. Workman" > Subject: [Histonet] QIHC > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > < > C9B2D9DC1CB4BF4CAAECB401F244490124F1D345@BLUPRD0511MB413.namprd05.prod.outlook.com > > > > Content-Type: text/plain; charset="iso-8859-1" > > I will be taking the QIHC soon has anyone taken it recently. I lost my > QIHC when I changed jobs and want to get > > it back. > > > Renee H. Workman > W: 804-527-1316 | F: 804-270-0917 > rhworkman@uro.com | www.uro.com< > http://www.uro.com/> > > > > > > Disclaimer: The email and files transmitted with it are confidential and > are intended solely for the use of the individual or entity to whom they > are addressed. If you are not the original recipient or the person > responsible for the delivering the email to the intended recipient, be > advised that you have received this email in error, and that any use, > dissemination, forwarding, printing or copying of this email is strictly > prohibited. If you received this email in error, please delete it from your > system without copying it, and notify the sender by reply email so that our > address record can be corrected. > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 112, Issue 1 > **************************************** > From turkekul <@t> gmail.com Fri Mar 1 19:03:12 2013 From: turkekul <@t> gmail.com (mesruh turkekul) Date: Fri Mar 1 19:03:17 2013 Subject: [Histonet] teeth sectioning Message-ID: Dear Histonetters, I have one more question. Is it possible to obtain 5-10um thick sections of PMMA embedded teeth using regular Leica paraffin microtome (RM2265) equipped with disposable tungsten carbide blade? Thanks, Mes On Fri, Mar 1, 2013 at 7:40 PM, mesruh turkekul wrote: > Dear Histonetters, > > > I am studying bone and teeth growth in rat maxilla. I will inject calcein > green and would like to fix, embed and sections the rat maxilla. > Any suggestions for the best method to fix, embed and section the samples > for fluorescnet microscopy? > > Thank you very much! > > Mes HTL (ASCP) > Memorial Sloan-Kettering Cancer Center > > On Fri, Mar 1, 2013 at 1:01 PM, < > histonet-request@lists.utsouthwestern.edu> wrote: > >> Send Histonet mailing list submissions to >> histonet@lists.utsouthwestern.edu >> >> To subscribe or unsubscribe via the World Wide Web, visit >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> or, via email, send a message with subject or body 'help' to >> histonet-request@lists.utsouthwestern.edu >> >> You can reach the person managing the list at >> histonet-owner@lists.utsouthwestern.edu >> >> When replying, please edit your Subject line so it is more specific >> than "Re: Contents of Histonet digest..." >> >> >> Today's Topics: >> >> 1. FNA Clia Guidelines (PicheGrocki, Jessica) >> 2. RE: FNA Clia Guidelines (Horn, Hazel V) >> 3. GSH Symposium April 12-14 (Zimmerman, Billie) >> 4. FSH abstracts deadline (Jerry Santiago, MSEd, HTL (ASCP) QIHC) >> 5. (no subject) (Vikrant Piprode) >> 6. The GSH 4oth Anniversary Meeting (David Kemler) >> 7. QIHC (Renee H. Workman) >> >> >> ---------------------------------------------------------------------- >> >> Message: 1 >> Date: Thu, 28 Feb 2013 20:26:30 +0000 >> From: "PicheGrocki, Jessica" >> Subject: [Histonet] FNA Clia Guidelines >> To: "histonet@lists.utsouthwestern.edu" >> >> Message-ID: >> < >> 631955447A364B45B9458D2905635110655D26B9@WIN08-MBX-01.wtbyhosp.org> >> Content-Type: text/plain; charset="us-ascii" >> >> Hi All, >> >> Quick question............what are the Clia requirements for Fine needle >> aspirate procedures? Is it considered high complexity testing? And who >> prepares the slides when the needle is handed off? >> >> Thank you, >> >> Jessica Piche, HT(ASCP) >> >> >> >> CONFIDENTIALITY NOTICE: This email and any attachments contain >> confidential information that is legally privileged. This information is >> intended only for the use of the individual or entity named above. The >> authorized recipient of this information is prohibited from disclosing this >> information to any other party unless required to do so by law or >> regulation. If you are not the intended recipient, you are hereby notified >> that any disclosure, copying, distribution or action taken in reliance on >> the contents of these documents is strictly prohibited. If you have >> received this information in error, please notify the sender immediately >> and delete these documents. Copyright (c) Waterbury Hospital >> >> >> ------------------------------ >> >> Message: 2 >> Date: Thu, 28 Feb 2013 15:05:35 -0600 >> From: "Horn, Hazel V" >> Subject: [Histonet] RE: FNA Clia Guidelines >> To: "'PicheGrocki, Jessica'" , >> "histonet@lists.utsouthwestern.edu" >> >> Message-ID: >> <25A4DE08332B19499904459F00AAACB719BE13564F@EVS1.archildrens.org> >> Content-Type: text/plain; charset="us-ascii" >> >> Our pathologists do it all. >> >> Hazel Horn >> Supervisor of Histology/Autopsy/Transcription >> Anatomic Pathology >> Arkansas Children's Hospital >> 1 Children's Way | Slot 820| Little Rock, AR 72202 >> 501.364.4240 direct | 501.364.1241 fax >> hornhv@archildrens.org >> archildrens.org >> >> >> >> >> >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu [mailto: >> histonet-bounces@lists.utsouthwestern.edu] On Behalf Of PicheGrocki, >> Jessica >> Sent: Thursday, February 28, 2013 2:27 PM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] FNA Clia Guidelines >> >> Hi All, >> >> Quick question............what are the Clia requirements for Fine needle >> aspirate procedures? Is it considered high complexity testing? And who >> prepares the slides when the needle is handed off? >> >> Thank you, >> >> Jessica Piche, HT(ASCP) >> >> >> >> CONFIDENTIALITY NOTICE: This email and any attachments contain >> confidential information that is legally privileged. This information is >> intended only for the use of the individual or entity named above. The >> authorized recipient of this information is prohibited from disclosing this >> information to any other party unless required to do so by law or >> regulation. If you are not the intended recipient, you are hereby notified >> that any disclosure, copying, distribution or action taken in reliance on >> the contents of these documents is strictly prohibited. If you have >> received this information in error, please notify the sender immediately >> and delete these documents. Copyright (c) Waterbury Hospital >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** >> The information contained in this message may be privileged and >> confidential >> and protected from disclosure. If the reader of this message is not the >> intended recipient, or an employee or agent responsible for delivering >> this >> message to the intended recipient, you are hereby notified that any >> dissemination, distribution or copying of this communication is strictly >> prohibited. If you have received this communication in error, please >> notify >> us immediately by replying to the message and deleting it from your >> computer. >> Thank you. >> >> >> >> ------------------------------ >> >> Message: 3 >> Date: Thu, 28 Feb 2013 21:41:34 +0000 >> From: "Zimmerman, Billie" >> Subject: [Histonet] GSH Symposium April 12-14 >> To: "histonet@lists.utsouthwestern.edu" >> >> Message-ID: >> < >> 7B3DEB32E69C034EACB479059C5DE3FF758BF9@EX-MLB-03.ad.georgiahealth.edu> >> >> Content-Type: text/plain; charset="us-ascii" >> >> Don't forget to register for the Georgia Society of Histotechnology >> meeting April 12-14. >> What a great getaway weekend to earn up to 15 CEU's from the NSH. Join us >> for education, >> networking, and some great scenery! >> >> Wanda Simons >> President of GSH >> >> /bz >> Augusta State University and Georgia Health Sciences University have >> consolidated to become Georgia Regents University. Effective January 9, >> 2013, my email address has changed to BZIMMERM@gru.edu. Please update >> your address book to reflect this change. >> >> >> ------------------------------ >> >> Message: 4 >> Date: Thu, 28 Feb 2013 20:51:06 -0500 >> From: "Jerry Santiago, MSEd, HTL (ASCP) QIHC" >> >> Subject: [Histonet] FSH abstracts deadline >> To: histonet@lists.utsouthwestern.edu >> Message-ID: >> Content-Type: text/plain; charset=utf-8 >> >> >> Dear histonetters, >> >> March 1st is the ddeadline to submit any abstracts consideration for the >> 2013 Florida Society for Histotechnology meeting. To submit an abstract go >> to www.fshgroup.org >> >> Or email to fsh@fshgroup.org >> >> Jerry Santiago >> FSH >> >> ------------------------------ >> >> Message: 5 >> Date: Fri, 1 Mar 2013 00:31:11 -0800 (PST) >> From: Vikrant Piprode >> Subject: [Histonet] (no subject) >> To: "histonet@lists.utsouthwestern.edu" >> >> Message-ID: >> <1362126671.40509.YahooMailNeo@web162904.mail.bf1.yahoo.com> >> Content-Type: text/plain; charset=iso-8859-1 >> >> hello, >> I have tried staining my calvarial bone sections for Tartrate Resistant >> Acid Phosphatase but it didnt work out. Can u help me out in this regard. >> >> Thank you >> Vikrant Piprode >> >> ------------------------------ >> >> Message: 6 >> Date: Fri, 1 Mar 2013 07:42:31 -0800 (PST) >> From: David Kemler >> Subject: [Histonet] The GSH 4oth Anniversary Meeting >> To: Fellow HistoNetters >> Message-ID: >> <1362152551.97608.YahooMailNeo@web121501.mail.ne1.yahoo.com> >> Content-Type: text/plain; charset=iso-8859-1 >> >> Hi Folks - Just a quick reminder that HistoTALK http://www.histotalk.com/has been invited by the GSH board to participate in the festivities again >> this year! The GSH meeting is always a great opportunity to help fulfill >> your CEU requirements attending workshops by the very best speakers and >> presenters in the histology field! >> >> I'm not presenting this year at the GSH, but I will be there with the >> HistoTALK "mobile" studio for guest interviews. >> >> Jekyll Island is a fantastic place for a state meeting and a great >> opportunity to mix and mingle. Be sure to join us for a way-cool time! GSH >> members are FUN folks! I know once you go to just one of their >> state meetings - you'll be hooked! >> >> The date is April 12th - 14th, so there's still time to register. >> >> And again, thanks GSH board for inviting me and HistoTALK to be part >> of your 40th Anniversary Celebration! >> >> Yours, >> David >> http://www.histotalk.com/ >> >> ------------------------------ >> >> Message: 7 >> Date: Fri, 1 Mar 2013 16:26:19 +0000 >> From: "Renee H. Workman" >> Subject: [Histonet] QIHC >> To: "histonet@lists.utsouthwestern.edu" >> >> Message-ID: >> < >> C9B2D9DC1CB4BF4CAAECB401F244490124F1D345@BLUPRD0511MB413.namprd05.prod.outlook.com >> > >> >> Content-Type: text/plain; charset="iso-8859-1" >> >> I will be taking the QIHC soon has anyone taken it recently. I lost my >> QIHC when I changed jobs and want to get >> >> it back. >> >> >> Renee H. Workman >> W: 804-527-1316 | F: 804-270-0917 >> rhworkman@uro.com | www.uro.com< >> http://www.uro.com/> >> >> >> >> >> >> Disclaimer: The email and files transmitted with it are confidential and >> are intended solely for the use of the individual or entity to whom they >> are addressed. If you are not the original recipient or the person >> responsible for the delivering the email to the intended recipient, be >> advised that you have received this email in error, and that any use, >> dissemination, forwarding, printing or copying of this email is strictly >> prohibited. If you received this email in error, please delete it from your >> system without copying it, and notify the sender by reply email so that our >> address record can be corrected. >> >> >> >> ------------------------------ >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> End of Histonet Digest, Vol 112, Issue 1 >> **************************************** >> > > From ratliffjack <@t> hotmail.com Fri Mar 1 20:11:29 2013 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Fri Mar 1 20:11:38 2013 Subject: [Histonet] teeth sectioning In-Reply-To: References: Message-ID: <1E916435-F342-4738-BDEC-1BAF7A0FB6C2@hotmail.com> Mes, This is a very good question and I look forward to answers from individuals that have accomplished this with PMMA and a rotary microtome with tungsten-carbide knives. If you are talking about an undecalcified specimen embedded in PMMA, then I would imagine that the age of the rat could affect the ability to achieve an adequate infiltration of the resin. Again, I look forward to what others have to say about their success by the method you have outlined. On the other hand, I know you can achieve the micron thickness you desire if you were to use a non-contact femtosecond laser! The machine I am talking about is basically a laser microtome manufactured by Rowiak in Germany and it is officially called the TissueSurgeon. In fact, Dr. Heiko Richter from Germany has accomplished what you ask with human teeth, revealing the full anatomy of the tooth and even with ameloblasts on the enamel surface! I would be interested to hear more about your project. I will be traveling to Germany one week from today to work with this laser microtome until the end of the month so I could arrange to have laser cut sections made for you if you are interested and unable to make your cuts using PMMA and a rotary microtome. If you would like more information, please feel free to contact me by email reply. Best Regards, Jack Jack L Ratliff Owner/Histologist, Ratliff Histology Consultants, LLC Chairman, Hard Tissue Committee - National Society for Histotechnology On Mar 1, 2013, at 7:03 PM, mesruh turkekul wrote: > Dear Histonetters, > > > I have one more question. Is it possible to obtain 5-10um thick sections of > PMMA embedded teeth using regular Leica paraffin microtome (RM2265) > equipped with disposable tungsten carbide blade? > > Thanks, > Mes > On Fri, Mar 1, 2013 at 7:40 PM, mesruh turkekul wrote: > >> Dear Histonetters, >> >> >> I am studying bone and teeth growth in rat maxilla. I will inject calcein >> green and would like to fix, embed and sections the rat maxilla. >> Any suggestions for the best method to fix, embed and section the samples >> for fluorescnet microscopy? >> >> Thank you very much! >> >> Mes HTL (ASCP) >> Memorial Sloan-Kettering Cancer Center >> >> On Fri, Mar 1, 2013 at 1:01 PM, < >> histonet-request@lists.utsouthwestern.edu> wrote: >> >>> Send Histonet mailing list submissions to >>> histonet@lists.utsouthwestern.edu >>> >>> To subscribe or unsubscribe via the World Wide Web, visit >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> or, via email, send a message with subject or body 'help' to >>> histonet-request@lists.utsouthwestern.edu >>> >>> You can reach the person managing the list at >>> histonet-owner@lists.utsouthwestern.edu >>> >>> When replying, please edit your Subject line so it is more specific >>> than "Re: Contents of Histonet digest..." >>> >>> >>> Today's Topics: >>> >>> 1. FNA Clia Guidelines (PicheGrocki, Jessica) >>> 2. RE: FNA Clia Guidelines (Horn, Hazel V) >>> 3. GSH Symposium April 12-14 (Zimmerman, Billie) >>> 4. FSH abstracts deadline (Jerry Santiago, MSEd, HTL (ASCP) QIHC) >>> 5. (no subject) (Vikrant Piprode) >>> 6. The GSH 4oth Anniversary Meeting (David Kemler) >>> 7. QIHC (Renee H. Workman) >>> >>> >>> ---------------------------------------------------------------------- >>> >>> Message: 1 >>> Date: Thu, 28 Feb 2013 20:26:30 +0000 >>> From: "PicheGrocki, Jessica" >>> Subject: [Histonet] FNA Clia Guidelines >>> To: "histonet@lists.utsouthwestern.edu" >>> >>> Message-ID: >>> < >>> 631955447A364B45B9458D2905635110655D26B9@WIN08-MBX-01.wtbyhosp.org> >>> Content-Type: text/plain; charset="us-ascii" >>> >>> Hi All, >>> >>> Quick question............what are the Clia requirements for Fine needle >>> aspirate procedures? Is it considered high complexity testing? And who >>> prepares the slides when the needle is handed off? >>> >>> Thank you, >>> >>> Jessica Piche, HT(ASCP) >>> >>> >>> >>> CONFIDENTIALITY NOTICE: This email and any attachments contain >>> confidential information that is legally privileged. This information is >>> intended only for the use of the individual or entity named above. The >>> authorized recipient of this information is prohibited from disclosing this >>> information to any other party unless required to do so by law or >>> regulation. If you are not the intended recipient, you are hereby notified >>> that any disclosure, copying, distribution or action taken in reliance on >>> the contents of these documents is strictly prohibited. If you have >>> received this information in error, please notify the sender immediately >>> and delete these documents. Copyright (c) Waterbury Hospital >>> >>> >>> ------------------------------ >>> >>> Message: 2 >>> Date: Thu, 28 Feb 2013 15:05:35 -0600 >>> From: "Horn, Hazel V" >>> Subject: [Histonet] RE: FNA Clia Guidelines >>> To: "'PicheGrocki, Jessica'" , >>> "histonet@lists.utsouthwestern.edu" >>> >>> Message-ID: >>> <25A4DE08332B19499904459F00AAACB719BE13564F@EVS1.archildrens.org> >>> Content-Type: text/plain; charset="us-ascii" >>> >>> Our pathologists do it all. >>> >>> Hazel Horn >>> Supervisor of Histology/Autopsy/Transcription >>> Anatomic Pathology >>> Arkansas Children's Hospital >>> 1 Children's Way | Slot 820| Little Rock, AR 72202 >>> 501.364.4240 direct | 501.364.1241 fax >>> hornhv@archildrens.org >>> archildrens.org >>> >>> >>> >>> >>> >>> >>> -----Original Message----- >>> From: histonet-bounces@lists.utsouthwestern.edu [mailto: >>> histonet-bounces@lists.utsouthwestern.edu] On Behalf Of PicheGrocki, >>> Jessica >>> Sent: Thursday, February 28, 2013 2:27 PM >>> To: histonet@lists.utsouthwestern.edu >>> Subject: [Histonet] FNA Clia Guidelines >>> >>> Hi All, >>> >>> Quick question............what are the Clia requirements for Fine needle >>> aspirate procedures? Is it considered high complexity testing? And who >>> prepares the slides when the needle is handed off? >>> >>> Thank you, >>> >>> Jessica Piche, HT(ASCP) >>> >>> >>> >>> CONFIDENTIALITY NOTICE: This email and any attachments contain >>> confidential information that is legally privileged. This information is >>> intended only for the use of the individual or entity named above. The >>> authorized recipient of this information is prohibited from disclosing this >>> information to any other party unless required to do so by law or >>> regulation. If you are not the intended recipient, you are hereby notified >>> that any disclosure, copying, distribution or action taken in reliance on >>> the contents of these documents is strictly prohibited. If you have >>> received this information in error, please notify the sender immediately >>> and delete these documents. Copyright (c) Waterbury Hospital >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >>> >>> ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** >>> The information contained in this message may be privileged and >>> confidential >>> and protected from disclosure. If the reader of this message is not the >>> intended recipient, or an employee or agent responsible for delivering >>> this >>> message to the intended recipient, you are hereby notified that any >>> dissemination, distribution or copying of this communication is strictly >>> prohibited. If you have received this communication in error, please >>> notify >>> us immediately by replying to the message and deleting it from your >>> computer. >>> Thank you. >>> >>> >>> >>> ------------------------------ >>> >>> Message: 3 >>> Date: Thu, 28 Feb 2013 21:41:34 +0000 >>> From: "Zimmerman, Billie" >>> Subject: [Histonet] GSH Symposium April 12-14 >>> To: "histonet@lists.utsouthwestern.edu" >>> >>> Message-ID: >>> < >>> 7B3DEB32E69C034EACB479059C5DE3FF758BF9@EX-MLB-03.ad.georgiahealth.edu> >>> >>> Content-Type: text/plain; charset="us-ascii" >>> >>> Don't forget to register for the Georgia Society of Histotechnology >>> meeting April 12-14. >>> What a great getaway weekend to earn up to 15 CEU's from the NSH. Join us >>> for education, >>> networking, and some great scenery! >>> >>> Wanda Simons >>> President of GSH >>> >>> /bz >>> Augusta State University and Georgia Health Sciences University have >>> consolidated to become Georgia Regents University. Effective January 9, >>> 2013, my email address has changed to BZIMMERM@gru.edu. Please update >>> your address book to reflect this change. >>> >>> >>> ------------------------------ >>> >>> Message: 4 >>> Date: Thu, 28 Feb 2013 20:51:06 -0500 >>> From: "Jerry Santiago, MSEd, HTL (ASCP) QIHC" >>> >>> Subject: [Histonet] FSH abstracts deadline >>> To: histonet@lists.utsouthwestern.edu >>> Message-ID: >>> Content-Type: text/plain; charset=utf-8 >>> >>> >>> Dear histonetters, >>> >>> March 1st is the ddeadline to submit any abstracts consideration for the >>> 2013 Florida Society for Histotechnology meeting. To submit an abstract go >>> to www.fshgroup.org >>> >>> Or email to fsh@fshgroup.org >>> >>> Jerry Santiago >>> FSH >>> >>> ------------------------------ >>> >>> Message: 5 >>> Date: Fri, 1 Mar 2013 00:31:11 -0800 (PST) >>> From: Vikrant Piprode >>> Subject: [Histonet] (no subject) >>> To: "histonet@lists.utsouthwestern.edu" >>> >>> Message-ID: >>> <1362126671.40509.YahooMailNeo@web162904.mail.bf1.yahoo.com> >>> Content-Type: text/plain; charset=iso-8859-1 >>> >>> hello, >>> I have tried staining my calvarial bone sections for Tartrate Resistant >>> Acid Phosphatase but it didnt work out. Can u help me out in this regard. >>> >>> Thank you >>> Vikrant Piprode >>> >>> ------------------------------ >>> >>> Message: 6 >>> Date: Fri, 1 Mar 2013 07:42:31 -0800 (PST) >>> From: David Kemler >>> Subject: [Histonet] The GSH 4oth Anniversary Meeting >>> To: Fellow HistoNetters >>> Message-ID: >>> <1362152551.97608.YahooMailNeo@web121501.mail.ne1.yahoo.com> >>> Content-Type: text/plain; charset=iso-8859-1 >>> >>> Hi Folks - Just a quick reminder that HistoTALK http://www.histotalk.com/has been invited by the GSH board to participate in the festivities again >>> this year! The GSH meeting is always a great opportunity to help fulfill >>> your CEU requirements attending workshops by the very best speakers and >>> presenters in the histology field! >>> >>> I'm not presenting this year at the GSH, but I will be there with the >>> HistoTALK "mobile" studio for guest interviews. >>> >>> Jekyll Island is a fantastic place for a state meeting and a great >>> opportunity to mix and mingle. Be sure to join us for a way-cool time! GSH >>> members are FUN folks! I know once you go to just one of their >>> state meetings - you'll be hooked! >>> >>> The date is April 12th - 14th, so there's still time to register. >>> >>> And again, thanks GSH board for inviting me and HistoTALK to be part >>> of your 40th Anniversary Celebration! >>> >>> Yours, >>> David >>> http://www.histotalk.com/ >>> >>> ------------------------------ >>> >>> Message: 7 >>> Date: Fri, 1 Mar 2013 16:26:19 +0000 >>> From: "Renee H. Workman" >>> Subject: [Histonet] QIHC >>> To: "histonet@lists.utsouthwestern.edu" >>> >>> Message-ID: >>> < >>> C9B2D9DC1CB4BF4CAAECB401F244490124F1D345@BLUPRD0511MB413.namprd05.prod.outlook.com >>> >>> Content-Type: text/plain; charset="iso-8859-1" >>> >>> I will be taking the QIHC soon has anyone taken it recently. I lost my >>> QIHC when I changed jobs and want to get >>> >>> it back. >>> >>> >>> Renee H. Workman >>> W: 804-527-1316 | F: 804-270-0917 >>> rhworkman@uro.com | www.uro.com< >>> http://www.uro.com/> >>> >>> >>> >>> >>> >>> Disclaimer: The email and files transmitted with it are confidential and >>> are intended solely for the use of the individual or entity to whom they >>> are addressed. If you are not the original recipient or the person >>> responsible for the delivering the email to the intended recipient, be >>> advised that you have received this email in error, and that any use, >>> dissemination, forwarding, printing or copying of this email is strictly >>> prohibited. If you received this email in error, please delete it from your >>> system without copying it, and notify the sender by reply email so that our >>> address record can be corrected. >>> >>> >>> >>> ------------------------------ >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >>> End of Histonet Digest, Vol 112, Issue 1 >>> **************************************** > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From rjbuesa <@t> yahoo.com Sat Mar 2 10:14:50 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Mar 2 10:14:54 2013 Subject: [Histonet] Ubiquitin Primary Antibody for IHC In-Reply-To: <5130E1D9020000230004B140@gwgwia1.luhs.org> References: <5130E1D9020000230004B140@gwgwia1.luhs.org> Message-ID: <1362240890.84945.YahooMailNeo@web163103.mail.bf1.yahoo.com> I used Dako with the DAKO autostainer. Ren? J. From: Roger Heyna To: histonet@lists.utsouthwestern.edu Sent: Friday, March 1, 2013 6:14 PM Subject: [Histonet] Ubiquitin Primary Antibody for IHC Our lab would like to routinely run the Ubiquitin IHC stain on formalin-fixed, paraffin-embedded brain and liver(mallory bodies), and I was wondering if anyone could recommend a primary antibody. We are currently using the Ventana Benchmark XT autostainer. Thank you, Roger Heyna Maywood, IL _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histotalk <@t> yahoo.com Sat Mar 2 20:15:30 2013 From: histotalk <@t> yahoo.com (David Kemler) Date: Sat Mar 2 20:16:03 2013 Subject: [Histonet] teeth sectioning In-Reply-To: <1E916435-F342-4738-BDEC-1BAF7A0FB6C2@hotmail.com> References: <1E916435-F342-4738-BDEC-1BAF7A0FB6C2@hotmail.com> Message-ID: <1362276930.75952.YahooMailNeo@web121502.mail.ne1.yahoo.com> ? Jack - ? I'm following your email up with an announcement once again about you and Dr. Heiko's interview on HistoTALK http://www.histotalk.com/. Maybe some of the folks out there?would like to hear?more?about your?involvment with laser histology. ? Have another great learning experience in Germany so that you can share?it all with us upon your return. I'll pencil you in for another guest interview at the Georgia Society for Histotechnology's?40th Anniversary Meeting in April. ? If you haven't heard Jack and Dr. Hieko's interview about laser?histology - it's a must listen! ? Yours, Dave ? Give Dr. my best!? ________________________________ From: Jack Ratliff To: mesruh turkekul Cc: "histonet@lists.utsouthwestern.edu" ; Jack Ratliff Sent: Friday, March 1, 2013 9:11 PM Subject: Re: [Histonet] teeth sectioning Mes, This is a very good question and I look forward to answers from individuals that have accomplished this with PMMA and a rotary microtome with tungsten-carbide knives. If you are talking about an undecalcified specimen embedded in PMMA, then I would imagine that the age of the rat could affect the ability to achieve an adequate infiltration of the resin. Again, I look forward to what others have to say about their success by the method you have outlined. On the other hand, I know you can achieve the micron thickness you desire if you were to use a non-contact femtosecond laser! The machine I am talking about is basically a laser microtome manufactured by Rowiak in Germany and it is officially called the TissueSurgeon. In fact, Dr. Heiko Richter from Germany has accomplished what you ask with human teeth, revealing the full anatomy of the tooth and even with ameloblasts on the enamel surface! I would be interested to hear more about your project. I will be traveling to Germany one week from today to work with this laser microtome until the end of the month so I could arrange to have laser cut sections made for you if you are interested and unable to make your cuts using PMMA and a rotary microtome. If you would like more information, please feel free to contact me by email reply. Best Regards, Jack Jack L Ratliff Owner/Histologist, Ratliff Histology Consultants, LLC Chairman, Hard Tissue Committee - National Society for Histotechnology On Mar 1, 2013, at 7:03 PM, mesruh turkekul wrote: > Dear Histonetters, > > > I have one more question. Is it possible to obtain 5-10um thick sections of > PMMA embedded teeth using regular Leica paraffin microtome (RM2265) > equipped with disposable tungsten carbide blade? > > Thanks, > Mes > On Fri, Mar 1, 2013 at 7:40 PM, mesruh turkekul wrote: > >> Dear Histonetters, >> >> >> I am studying bone and teeth growth in rat maxilla. I will inject calcein >> green and would like to fix, embed and sections the rat maxilla. >> Any suggestions for the best method to fix, embed and section the samples >> for fluorescnet microscopy? >> >> Thank you very much! >> >> Mes HTL (ASCP) >> Memorial Sloan-Kettering Cancer Center >> >> On Fri, Mar 1, 2013 at 1:01 PM, < >> histonet-request@lists.utsouthwestern.edu> wrote: >> >>> Send Histonet mailing list submissions to >>>? ? ? ? histonet@lists.utsouthwestern.edu >>> >>> To subscribe or unsubscribe via the World Wide Web, visit >>>? ? ? ? http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> or, via email, send a message with subject or body 'help' to >>>? ? ? ? histonet-request@lists.utsouthwestern.edu >>> >>> You can reach the person managing the list at >>>? ? ? ? histonet-owner@lists.utsouthwestern.edu >>> >>> When replying, please edit your Subject line so it is more specific >>> than "Re: Contents of Histonet digest..." >>> >>> >>> Today's Topics: >>> >>>? 1. FNA Clia Guidelines (PicheGrocki, Jessica) >>>? 2. RE: FNA Clia Guidelines (Horn, Hazel V) >>>? 3. GSH Symposium April 12-14 (Zimmerman, Billie) >>>? 4. FSH abstracts deadline (Jerry Santiago, MSEd, HTL (ASCP) QIHC) >>>? 5. (no subject) (Vikrant Piprode) >>>? 6. The GSH 4oth Anniversary Meeting (David Kemler) >>>? 7. QIHC (Renee H. Workman) >>> >>> >>> ---------------------------------------------------------------------- >>> >>> Message: 1 >>> Date: Thu, 28 Feb 2013 20:26:30 +0000 >>> From: "PicheGrocki, Jessica" >>> Subject: [Histonet] FNA Clia Guidelines >>> To: "histonet@lists.utsouthwestern.edu" >>>? ? ? ? >>> Message-ID: >>>? ? ? ? < >>> 631955447A364B45B9458D2905635110655D26B9@WIN08-MBX-01.wtbyhosp.org> >>> Content-Type: text/plain; charset="us-ascii" >>> >>> Hi All, >>> >>> Quick question............what are the Clia requirements for Fine needle >>> aspirate procedures? Is it considered high complexity testing? And who >>> prepares the slides when the needle is handed off? >>> >>> Thank you, >>> >>> Jessica Piche, HT(ASCP) >>> >>> >>> >>> CONFIDENTIALITY NOTICE: This email and any attachments contain >>> confidential information that is legally privileged. This information is >>> intended only for the use of the individual or entity named above. The >>> authorized recipient of this information is prohibited from disclosing this >>> information to any other party unless required to do so by law or >>> regulation. If you are not the intended recipient, you are hereby notified >>> that any disclosure, copying, distribution or action taken in reliance on >>> the contents of these documents is strictly prohibited. If you have >>> received this information in error, please notify the sender immediately >>> and delete these documents. Copyright (c) Waterbury Hospital >>> >>> >>> ------------------------------ >>> >>> Message: 2 >>> Date: Thu, 28 Feb 2013 15:05:35 -0600 >>> From: "Horn, Hazel V" >>> Subject: [Histonet] RE: FNA Clia Guidelines >>> To: "'PicheGrocki, Jessica'" , >>>? ? ? ? "histonet@lists.utsouthwestern.edu" >>>? ? ? ? >>> Message-ID: >>>? ? ? ? <25A4DE08332B19499904459F00AAACB719BE13564F@EVS1.archildrens.org> >>> Content-Type: text/plain; charset="us-ascii" >>> >>> Our pathologists do it all. >>> >>> Hazel Horn >>> Supervisor of Histology/Autopsy/Transcription >>> Anatomic Pathology >>> Arkansas Children's Hospital >>> 1 Children's Way | Slot 820| Little Rock, AR 72202 >>> 501.364.4240 direct | 501.364.1241 fax >>> hornhv@archildrens.org >>> archildrens.org >>> >>> >>> >>> >>> >>> >>> -----Original Message----- >>> From: histonet-bounces@lists.utsouthwestern.edu [mailto: >>> histonet-bounces@lists.utsouthwestern.edu] On Behalf Of PicheGrocki, >>> Jessica >>> Sent: Thursday, February 28, 2013 2:27 PM >>> To: histonet@lists.utsouthwestern.edu >>> Subject: [Histonet] FNA Clia Guidelines >>> >>> Hi All, >>> >>> Quick question............what are the Clia requirements for Fine needle >>> aspirate procedures? Is it considered high complexity testing? And who >>> prepares the slides when the needle is handed off? >>> >>> Thank you, >>> >>> Jessica Piche, HT(ASCP) >>> >>> >>> >>> CONFIDENTIALITY NOTICE: This email and any attachments contain >>> confidential information that is legally privileged. This information is >>> intended only for the use of the individual or entity named above. The >>> authorized recipient of this information is prohibited from disclosing this >>> information to any other party unless required to do so by law or >>> regulation. If you are not the intended recipient, you are hereby notified >>> that any disclosure, copying, distribution or action taken in reliance on >>> the contents of these documents is strictly prohibited. If you have >>> received this information in error, please notify the sender immediately >>> and delete these documents. Copyright (c) Waterbury Hospital >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >>> >>> ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** >>> The information contained in this message may be privileged and >>> confidential >>> and protected from disclosure. If the reader of this message is not the >>> intended recipient, or an employee or agent responsible for delivering >>> this >>> message to the intended recipient, you are hereby notified that any >>> dissemination, distribution or copying of this communication is strictly >>> prohibited. If you have received this communication in error, please >>> notify >>> us immediately by replying to the message and deleting it from your >>> computer. >>> Thank you. >>> >>> >>> >>> ------------------------------ >>> >>> Message: 3 >>> Date: Thu, 28 Feb 2013 21:41:34 +0000 >>> From: "Zimmerman, Billie" >>> Subject: [Histonet] GSH Symposium April 12-14 >>> To: "histonet@lists.utsouthwestern.edu" >>>? ? ? ? >>> Message-ID: >>>? ? ? ? < >>> 7B3DEB32E69C034EACB479059C5DE3FF758BF9@EX-MLB-03.ad.georgiahealth.edu> >>> >>> Content-Type: text/plain; charset="us-ascii" >>> >>> Don't forget to register for the Georgia Society of Histotechnology >>> meeting April 12-14. >>> What a great getaway weekend to earn up to 15 CEU's from the NSH. Join us >>> for education, >>> networking, and some great scenery! >>> >>> Wanda Simons >>> President of GSH >>> >>> /bz >>> Augusta State University and Georgia Health Sciences University have >>> consolidated to become Georgia Regents University. Effective January 9, >>> 2013, my email address has changed to BZIMMERM@gru.edu. Please update >>> your address book to reflect this change. >>> >>> >>> ------------------------------ >>> >>> Message: 4 >>> Date: Thu, 28 Feb 2013 20:51:06 -0500 >>> From: "Jerry Santiago, MSEd, HTL (ASCP) QIHC" >>>? ? ? ? >>> Subject: [Histonet] FSH abstracts deadline >>> To: histonet@lists.utsouthwestern.edu >>> Message-ID: >>> Content-Type: text/plain; charset=utf-8 >>> >>> >>> Dear histonetters, >>> >>> March 1st is the ddeadline to submit any abstracts consideration for the >>> 2013 Florida Society for Histotechnology meeting. To submit an abstract go >>> to www.fshgroup.org >>> >>> Or email to fsh@fshgroup.org >>> >>> Jerry Santiago >>> FSH >>> >>> ------------------------------ >>> >>> Message: 5 >>> Date: Fri, 1 Mar 2013 00:31:11 -0800 (PST) >>> From: Vikrant Piprode >>> Subject: [Histonet] (no subject) >>> To: "histonet@lists.utsouthwestern.edu" >>>? ? ? ? >>> Message-ID: >>>? ? ? ? <1362126671.40509.YahooMailNeo@web162904.mail.bf1.yahoo.com> >>> Content-Type: text/plain; charset=iso-8859-1 >>> >>> hello, >>> I have tried staining my calvarial bone sections for Tartrate Resistant >>> Acid Phosphatase but it didnt work out. Can u help me out in this regard. >>> >>> Thank you >>> Vikrant Piprode >>> >>> ------------------------------ >>> >>> Message: 6 >>> Date: Fri, 1 Mar 2013 07:42:31 -0800 (PST) >>> From: David Kemler >>> Subject: [Histonet] The GSH 4oth Anniversary Meeting >>> To: Fellow HistoNetters >>> Message-ID: >>>? ? ? ? <1362152551.97608.YahooMailNeo@web121501.mail.ne1.yahoo.com> >>> Content-Type: text/plain; charset=iso-8859-1 >>> >>> Hi Folks - Just a quick reminder that HistoTALK http://www.histotalk.com/has been invited by the GSH board to participate in the festivities again >>> this year! The GSH meeting is always a great opportunity to help fulfill >>> your CEU requirements attending workshops by the very best speakers and >>> presenters in the histology field! >>> >>> I'm not presenting this year at the GSH, but I will be there with the >>> HistoTALK "mobile" studio for guest interviews. >>> >>> Jekyll Island is a fantastic place for a state meeting and a great >>> opportunity to mix and mingle. Be sure to join us for a way-cool time! GSH >>> members are FUN folks! I know once you go to just one of their >>> state meetings - you'll be hooked! >>> >>> The date is April 12th - 14th, so there's still time to register. >>> >>> And again, thanks GSH board for inviting me and HistoTALK to be part >>> of your 40th Anniversary Celebration! >>> >>> Yours, >>> David >>> http://www.histotalk.com/ >>> >>> ------------------------------ >>> >>> Message: 7 >>> Date: Fri, 1 Mar 2013 16:26:19 +0000 >>> From: "Renee H. Workman" >>> Subject: [Histonet] QIHC >>> To: "histonet@lists.utsouthwestern.edu" >>>? ? ? ? >>> Message-ID: >>>? ? ? ? < >>> C9B2D9DC1CB4BF4CAAECB401F244490124F1D345@BLUPRD0511MB413.namprd05.prod.outlook.com >>> >>> Content-Type: text/plain; charset="iso-8859-1" >>> >>> I will be taking the QIHC soon has anyone taken it recently.? I lost my >>> QIHC when I changed jobs and want to get >>> >>> it back. >>> >>> >>> Renee H. Workman >>> W: 804-527-1316 | F: 804-270-0917 >>> rhworkman@uro.com | www.uro.com< >>> http://www.uro.com/> >>> >>> >>> >>> >>> >>> Disclaimer: The email and files transmitted with it are confidential and >>> are intended solely for the use of the individual or entity to whom they >>> are addressed. If you are not the original recipient or the person >>> responsible for the delivering the email to the intended recipient, be >>> advised that you have received this email in error, and that any use, >>> dissemination, forwarding, printing or copying of this email is strictly >>> prohibited. If you received this email in error, please delete it from your >>> system without copying it, and notify the sender by reply email so that our >>> address record can be corrected. >>> >>> >>> >>> ------------------------------ >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >>> End of Histonet Digest, Vol 112, Issue 1 >>> **************************************** > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jstaruk <@t> masshistology.com Sun Mar 3 12:03:37 2013 From: jstaruk <@t> masshistology.com (JStaruk) Date: Sun Mar 3 12:03:46 2013 Subject: [Histonet] Histology Supervisor - New England Message-ID: <001601ce1839$6f040520$4d0c0f60$@masshistology.com> HISTOLOGIST SUPERVISOR ? We have a unique position available at Mass Histology Service, Inc. Mass Histo is a busy, well established, rapidly growing, highly reputable private histopathology lab located in central MA. We are looking for an experienced histologist with a strong background in performing and supervising routine and special stains, frozen sections, immunohistochemistry, tissue trimming (grossing) and general histology procedures. We are a GLP-compliant lab and the applicant must be HT(ASCP) licensed. There is a strong possibility of a partnership with future ownership for the right applicant. We offer great benefits and a very pleasant and relaxed work environment. Starting annual salary is $90,000 for the right person with the right experience. Please e-mail your cover letter and resume to info@masshistology.com. Please, no recruiters. _______________________ James E.?Staruk HT(ASCP) ?www.masshistology.com ?? www.nehorselabs.com From kjgada <@t> gmail.com Sun Mar 3 19:13:45 2013 From: kjgada <@t> gmail.com (Komal Gada) Date: Sun Mar 3 19:13:49 2013 Subject: [Histonet] Internship Opportunities Wanted Message-ID: Hello Histo-netters, I am writing once again on the behalf of Keiser University's Associates in Histotechnology program. The education of future histotechs through this program consists of eight months of classroom / lab instruction followed by two months of a clinical rotatation (internship). Currently, we partner with many facilities in Central Florida to ensure our students receive the best possible experience and graduate as competent Histotechs. I am reaching out to offer this opportunity to partner with Keiser University as a possible internship site for anyone interested. In becoming an affiliate site, you would agree to accept a Histotechnology student that meets your approval, and host them through their internship. In return, you will gain full time assistance for eight weeks, at *no cost to you* (other than a little guidance, as needed). All in all, it is a win-win situation all around. Many facilities have found that the externship program is an excellent way to screen potential new employees. There is an opportunity to freshly mold the student and train him or her in a way that is specific to the facility, while gaining assistance for 2 months at no cost. Employment is never implied, though it is always an option, should the student be the perfect fit for your facility. All in all, the program is a great way to determine if an individual will work well with your staff before making any kind of commitment. If you are interested in having *Histotechnology students* volunteer in your facility, would like more information, or have any questions, please email me, and I will respond as quickly as possible. While most of the students prefer Florida sites, we do have students who request to complete internships throughout the nation. I look forward to hearing from you, Komal * * *Komal Gada, BS, HT/HTL(ASCP), QIHC** **Histotechnology Program Director * From John.Garratt <@t> vch.ca Sun Mar 3 20:55:06 2013 From: John.Garratt <@t> vch.ca (Garratt, John [NS]) Date: Sun Mar 3 20:55:15 2013 Subject: [Histonet] Ethics of using human tissue for quality control in histology Message-ID: Learn more at the CIQC/CAP-ACP SEMINAR: DIAGNOSTIC ihc AND MOLECULAR PATHOLOGY For registration go to: http://ciqc-capconference.eventbrite.ca/ From lpwenk <@t> sbcglobal.net Mon Mar 4 04:43:43 2013 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Mon Mar 4 04:43:47 2013 Subject: [Histonet] QIHC In-Reply-To: References: Message-ID: <1BBF03EC760C49C98F460DA5EC7D7B02@HP2010> Just to let everyone know - the Michigan Society of Histotechnologists have put together a Study Guide/Workbook, geared to help those studying for the QIHC. It is a workbook, where you have to look up the information in books, and write it in the booklet. There are no answers provided (it's not a multiple choice question book, it's writing out definitions and how procedures work). This workbook is tries to cover all the areas on the exam, as listed on the ASCP BOC QIHC exam content outline. It is to help people get organized in their studying. $20. For more information, go to the MSH webpage: www.mihisto.org Click on Education Click on Study Guides Print out the order form at the bottom. Disclosure statement: I am a member of MSH. I do not receive any money from the sale of the QIHC Study Guide. Any money made (after printing and mailing costs) goes to support MSH. Peggy A. Wenk, HTL(ASCP)SLS -----Original Message----- From: Renee H. Workman Sent: Friday, March 01, 2013 11:26 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] QIHC I will be taking the QIHC soon has anyone taken it recently. I lost my QIHC when I changed jobs and want to get it back. Renee H. Workman W: 804-527-1316 | F: 804-270-0917 rhworkman@uro.com | www.uro.com Disclaimer: The email and files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the original recipient or the person responsible for the delivering the email to the intended recipient, be advised that you have received this email in error, and that any use, dissemination, forwarding, printing or copying of this email is strictly prohibited. If you received this email in error, please delete it from your system without copying it, and notify the sender by reply email so that our address record can be corrected. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpwenk <@t> sbcglobal.net Mon Mar 4 04:49:12 2013 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Mon Mar 4 04:49:37 2013 Subject: [Histonet] RESCUING UNDER DECALCIFIED BONE FROM PARAFFIN EMBEDDEDBLOCKS In-Reply-To: References: Message-ID: Can you surface decal? After facing the block on the microtome, getting a full face, pour some decalcification fluid in the lid of a coplin jar. Place the block faced side down in the decal solution, for about 30 minutes. Rinse the acid off the block with some cool water (don't want acid dripping on the microtome blade holder and blade). Line the block up exactly on the microtome to the knife. The first 2-4 sections will be decalcified enough for you to cut. (That's why the block has to be lined up exactly - can't waste rough trimming off the 10-20 um of decalcified tissue.) Peggy A. Wenk, HTL(ASCP)SLS Beaumont Health System Royal Oak, MI 48073 The opinions expressed are mine, and do not reflect on my place of employment. -----Original Message----- From: Fiona J Morrow Sent: Thursday, February 28, 2013 8:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RESCUING UNDER DECALCIFIED BONE FROM PARAFFIN EMBEDDEDBLOCKS Hi Has anyone ever tried to reprocess bone tissue that has been under decalcified and processed through to wax? Thanks Fi M Fiona Morrow Dept. of Infection and Immunity KFloor, Room K118 Medical School University of Sheffield Beech Hill Road Sheffield S10 2RX 0114 271 2102 F.morrow@sheffield.ac.uk _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpwenk <@t> sbcglobal.net Mon Mar 4 05:08:13 2013 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Mon Mar 4 05:08:17 2013 Subject: [Histonet] Unencased Ameoba Stain In-Reply-To: References: Message-ID: <6802396816914A11BAFB2B9D65A0D63C@HP2010> Getting back a little late (been out of state). If this is Acanthamoeba, then we do a PASH and/or a GMS. These are cysts, and show up nicely with both. The cornea can be PAS positive also, so the Acanthamoeba are a darker pink against a lighter pink, while the GMS is gray/black against the green background. It looks a lot like pneumocystis with a GMS, but without the bull's eye, and about 2-3 times larger. But it's the cyst wall that is staining with the PAS and the GMS, as the cyst has a lot of glycogen. But you are asking for unencased amoeba. So that sounds like no cyst. Are you looking for just the trophozoites? I would suggest one of the Giemsa stains, or maybe a Brown and Hopps. A cresyl echt violet (actually cresyl violet acetate, but everyone still calls it by it's old name), would probably also work - I read about it in the NSH Journal of Histotechnology about 20 years ago for the trophozoites of pneumocystis, but I've never tried the stain. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Health System Royal Oak, MI 48073 The opinions expressed are mine, and do not represent BHS. -----Original Message----- From: Joseph Brooks Sent: Wednesday, February 27, 2013 11:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Unencased Ameoba Stain Hello All, One of our Neuropath docs is inquiring about a special stain for unencased ameobas in cornea biopsies. I did a search and Gridley's Method was the best option that appreaded. Is there someone that could either verify this stain will work on this organism or let me know what you stain you are using? Thanks in advance. Matt Brooks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ewj <@t> pigsqq.org Mon Mar 4 05:47:35 2013 From: ewj <@t> pigsqq.org (=?UTF-8?B?IkUuIFdheW5lIEpvaG5zb24g5pyx56iz5qOuIg==?=) Date: Mon Mar 4 05:47:45 2013 Subject: [Histonet] teeth sectioning In-Reply-To: <1E916435-F342-4738-BDEC-1BAF7A0FB6C2@hotmail.com> References: <1E916435-F342-4738-BDEC-1BAF7A0FB6C2@hotmail.com> Message-ID: <513489D7.2020804@pigsqq.org> Jack, That sounds really awesome. I did some work with the teeth of sows (female pigs) from specimens collected at slaughter. Those are very difficult to decalcify, and when finished, are likely to have no nuclear detail remaining. Interested to hear what you learn Wayne Johnson Beijing Enable Ag Consulting Yuanmingyuan West Road Meiyuan Com, On 3:59, Jack Ratliff wrote: > Mes, > > This is a very good question and I look forward to answers from individuals that have accomplished this with PMMA and a rotary microtome with tungsten-carbide knives. If you are talking about an undecalcified specimen embedded in PMMA, then I would imagine that the age of the rat could affect the ability to achieve an adequate infiltration of the resin. Again, I look forward to what others have to say about their success by the method you have outlined. > > On the other hand, I know you can achieve the micron thickness you desire if you were to use a non-contact femtosecond laser! The machine I am talking about is basically a laser microtome manufactured by Rowiak in Germany and it is officially called the TissueSurgeon. In fact, Dr. Heiko Richter from Germany has accomplished what you ask with human teeth, revealing the full anatomy of the tooth and even with ameloblasts on the enamel surface! > > I would be interested to hear more about your project. I will be traveling to Germany one week from today to work with this laser microtome until the end of the month so I could arrange to have laser cut sections made for you if you are interested and unable to make your cuts using PMMA and a rotary microtome. If you would like more information, please feel free to contact me by email reply. > > Best Regards, > > Jack > > > > Jack L Ratliff > Owner/Histologist, Ratliff Histology Consultants, LLC > Chairman, Hard Tissue Committee - National Society for Histotechnology > > > > On Mar 1, 2013, at 7:03 PM, mesruh turkekul wrote: > > >> Dear Histonetters, >> >> >> I have one more question. Is it possible to obtain 5-10um thick sections of >> PMMA embedded teeth using regular Leica paraffin microtome (RM2265) >> equipped with disposable tungsten carbide blade? >> >> Thanks, >> Mes >> On Fri, Mar 1, 2013 at 7:40 PM, mesruh turkekul wrote: >> >> >>> Dear Histonetters, >>> >>> >>> I am studying bone and teeth growth in rat maxilla. I will inject calcein >>> green and would like to fix, embed and sections the rat maxilla. >>> Any suggestions for the best method to fix, embed and section the samples >>> for fluorescnet microscopy? >>> >>> Thank you very much! >>> >>> Mes HTL (ASCP) >>> Memorial Sloan-Kettering Cancer Center >>> >>> On Fri, Mar 1, 2013 at 1:01 PM,< >>> histonet-request@lists.utsouthwestern.edu> wrote: >>> >>> >>>> Send Histonet mailing list submissions to >>>> histonet@lists.utsouthwestern.edu >>>> >>>> To subscribe or unsubscribe via the World Wide Web, visit >>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>>> or, via email, send a message with subject or body 'help' to >>>> histonet-request@lists.utsouthwestern.edu >>>> >>>> You can reach the person managing the list at >>>> histonet-owner@lists.utsouthwestern.edu >>>> >>>> When replying, please edit your Subject line so it is more specific >>>> than "Re: Contents of Histonet digest..." >>>> >>>> >>>> Today's Topics: >>>> >>>> 1. FNA Clia Guidelines (PicheGrocki, Jessica) >>>> 2. RE: FNA Clia Guidelines (Horn, Hazel V) >>>> 3. GSH Symposium April 12-14 (Zimmerman, Billie) >>>> 4. FSH abstracts deadline (Jerry Santiago, MSEd, HTL (ASCP) QIHC) >>>> 5. (no subject) (Vikrant Piprode) >>>> 6. The GSH 4oth Anniversary Meeting (David Kemler) >>>> 7. QIHC (Renee H. Workman) >>>> >>>> >>>> ---------------------------------------------------------------------- >>>> >>>> Message: 1 >>>> Date: Thu, 28 Feb 2013 20:26:30 +0000 >>>> From: "PicheGrocki, Jessica" >>>> Subject: [Histonet] FNA Clia Guidelines >>>> To: "histonet@lists.utsouthwestern.edu" >>>> >>>> Message-ID: >>>> < >>>> 631955447A364B45B9458D2905635110655D26B9@WIN08-MBX-01.wtbyhosp.org> >>>> Content-Type: text/plain; charset=s-ascii" >>>> >>>> Hi All, >>>> >>>> Quick question............what are the Clia requirements for Fine needle >>>> aspirate procedures? Is it considered high complexity testing? And who >>>> prepares the slides when the needle is handed off? >>>> >>>> Thank you, >>>> >>>> Jessica Piche, HT(ASCP) >>>> >>>> >>>> >>>> CONFIDENTIALITY NOTICE: This email and any attachments contain >>>> confidential information that is legally privileged. This information is >>>> intended only for the use of the individual or entity named above. The >>>> authorized recipient of this information is prohibited from disclosing this >>>> information to any other party unless required to do so by law or >>>> regulation. If you are not the intended recipient, you are hereby notified >>>> that any disclosure, copying, distribution or action taken in reliance on >>>> the contents of these documents is strictly prohibited. If you have >>>> received this information in error, please notify the sender immediately >>>> and delete these documents. Copyright (c) Waterbury Hospital >>>> >>>> >>>> ------------------------------ >>>> >>>> Message: 2 >>>> Date: Thu, 28 Feb 2013 15:05:35 -0600 >>>> From: "Horn, Hazel V" >>>> Subject: [Histonet] RE: FNA Clia Guidelines >>>> To: "'PicheGrocki, Jessica'", >>>> "histonet@lists.utsouthwestern.edu" >>>> >>>> Message-ID: >>>> <25A4DE08332B19499904459F00AAACB719BE13564F@EVS1.archildrens.org> >>>> Content-Type: text/plain; charset=s-ascii" >>>> >>>> Our pathologists do it all. >>>> >>>> Hazel Horn >>>> Supervisor of Histology/Autopsy/Transcription >>>> Anatomic Pathology >>>> Arkansas Children's Hospital >>>> 1 Children's Way | Slot 820| Little Rock, AR 72202 >>>> 501.364.4240 direct | 501.364.1241 fax >>>> hornhv@archildrens.org >>>> archildrens.org >>>> >>>> >>>> >>>> >>>> >>>> >>>> -----Original Message----- >>>> From: histonet-bounces@lists.utsouthwestern.edu [mailto: >>>> histonet-bounces@lists.utsouthwestern.edu] On Behalf Of PicheGrocki, >>>> Jessica >>>> Sent: Thursday, February 28, 2013 2:27 PM >>>> To: histonet@lists.utsouthwestern.edu >>>> Subject: [Histonet] FNA Clia Guidelines >>>> >>>> Hi All, >>>> >>>> Quick question............what are the Clia requirements for Fine needle >>>> aspirate procedures? Is it considered high complexity testing? And who >>>> prepares the slides when the needle is handed off? >>>> >>>> Thank you, >>>> >>>> Jessica Piche, HT(ASCP) >>>> >>>> >>>> >>>> CONFIDENTIALITY NOTICE: This email and any attachments contain >>>> confidential information that is legally privileged. This information is >>>> intended only for the use of the individual or entity named above. The >>>> authorized recipient of this information is prohibited from disclosing this >>>> information to any other party unless required to do so by law or >>>> regulation. If you are not the intended recipient, you are hereby notified >>>> that any disclosure, copying, distribution or action taken in reliance on >>>> the contents of these documents is strictly prohibited. If you have >>>> received this information in error, please notify the sender immediately >>>> and delete these documents. Copyright (c) Waterbury Hospital >>>> _______________________________________________ >>>> Histonet mailing list >>>> Histonet@lists.utsouthwestern.edu >>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>>> >>>> >>>> ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** >>>> The information contained in this message may be privileged and >>>> confidential >>>> and protected from disclosure. If the reader of this message is not the >>>> intended recipient, or an employee or agent responsible for delivering >>>> this >>>> message to the intended recipient, you are hereby notified that any >>>> dissemination, distribution or copying of this communication is strictly >>>> prohibited. If you have received this communication in error, please >>>> notify >>>> us immediately by replying to the message and deleting it from your >>>> computer. >>>> Thank you. >>>> >>>> >>>> >>>> ------------------------------ >>>> >>>> Message: 3 >>>> Date: Thu, 28 Feb 2013 21:41:34 +0000 >>>> From: "Zimmerman, Billie" >>>> Subject: [Histonet] GSH Symposium April 12-14 >>>> To: "histonet@lists.utsouthwestern.edu" >>>> >>>> Message-ID: >>>> < >>>> 7B3DEB32E69C034EACB479059C5DE3FF758BF9@EX-MLB-03.ad.georgiahealth.edu> >>>> >>>> Content-Type: text/plain; charset=s-ascii" >>>> >>>> Don't forget to register for the Georgia Society of Histotechnology >>>> meeting April 12-14. >>>> What a great getaway weekend to earn up to 15 CEU's from the NSH. Join us >>>> for education, >>>> networking, and some great scenery! >>>> >>>> Wanda Simons >>>> President of GSH >>>> >>>> /bz >>>> Augusta State University and Georgia Health Sciences University have >>>> consolidated to become Georgia Regents University. Effective January 9, >>>> 2013, my email address has changed to BZIMMERM@gru.edu. Please update >>>> your address book to reflect this change. >>>> >>>> >>>> ------------------------------ >>>> >>>> Message: 4 >>>> Date: Thu, 28 Feb 2013 20:51:06 -0500 >>>> From: "Jerry Santiago, MSEd, HTL (ASCP) QIHC" >>>> >>>> Subject: [Histonet] FSH abstracts deadline >>>> To: histonet@lists.utsouthwestern.edu >>>> Message-ID: >>>> Content-Type: text/plain; charset=f-8 >>>> >>>> >>>> Dear histonetters, >>>> >>>> March 1st is the ddeadline to submit any abstracts consideration for the >>>> 2013 Florida Society for Histotechnology meeting. To submit an abstract go >>>> to www.fshgroup.org >>>> >>>> Or email to fsh@fshgroup.org >>>> >>>> Jerry Santiago >>>> FSH >>>> >>>> ------------------------------ >>>> >>>> Message: 5 >>>> Date: Fri, 1 Mar 2013 00:31:11 -0800 (PST) >>>> From: Vikrant Piprode >>>> Subject: [Histonet] (no subject) >>>> To: "histonet@lists.utsouthwestern.edu" >>>> >>>> Message-ID: >>>> <1362126671.40509.YahooMailNeo@web162904.mail.bf1.yahoo.com> >>>> Content-Type: text/plain; charset=o-8859-1 >>>> >>>> hello, >>>> I have tried staining my calvarial bone sections for Tartrate Resistant >>>> Acid Phosphatase but it didnt work out. Can u help me out in this regard. >>>> >>>> Thank you >>>> Vikrant Piprode >>>> >>>> ------------------------------ >>>> >>>> Message: 6 >>>> Date: Fri, 1 Mar 2013 07:42:31 -0800 (PST) >>>> From: David Kemler >>>> Subject: [Histonet] The GSH 4oth Anniversary Meeting >>>> To: Fellow HistoNetters >>>> Message-ID: >>>> <1362152551.97608.YahooMailNeo@web121501.mail.ne1.yahoo.com> >>>> Content-Type: text/plain; charset=o-8859-1 >>>> >>>> Hi Folks - Just a quick reminder that HistoTALK http://www.histotalk.com/has been invited by the GSH board to participate in the festivities again >>>> this year! The GSH meeting is always a great opportunity to help fulfill >>>> your CEU requirements attending workshops by the very best speakers and >>>> presenters in the histology field! >>>> >>>> I'm not presenting this year at the GSH, but I will be there with the >>>> HistoTALK "mobile" studio for guest interviews. >>>> >>>> Jekyll Island is a fantastic place for a state meeting and a great >>>> opportunity to mix and mingle. Be sure to join us for a way-cool time! GSH >>>> members are FUN folks! I know once you go to just one of their >>>> state meetings - you'll be hooked! >>>> >>>> The date is April 12th - 14th, so there's still time to register. >>>> >>>> And again, thanks GSH board for inviting me and HistoTALK to be part >>>> of your 40th Anniversary Celebration! >>>> >>>> Yours, >>>> David >>>> http://www.histotalk.com/ >>>> >>>> ------------------------------ >>>> >>>> Message: 7 >>>> Date: Fri, 1 Mar 2013 16:26:19 +0000 >>>> From: "Renee H. Workman" >>>> Subject: [Histonet] QIHC >>>> To: "histonet@lists.utsouthwestern.edu" >>>> >>>> Message-ID: >>>> < >>>> C9B2D9DC1CB4BF4CAAECB401F244490124F1D345@BLUPRD0511MB413.namprd05.prod.outlook.com >>>> >>>> Content-Type: text/plain; charset=so-8859-1" >>>> >>>> I will be taking the QIHC soon has anyone taken it recently. I lost my >>>> QIHC when I changed jobs and want to get >>>> >>>> it back. >>>> >>>> >>>> Renee H. Workman >>>> W: 804-527-1316 | F: 804-270-0917 >>>> rhworkman@uro.com | www.uro.com< >>>> http://www.uro.com/> >>>> >>>> >>>> >>>> >>>> >>>> Disclaimer: The email and files transmitted with it are confidential and >>>> are intended solely for the use of the individual or entity to whom they >>>> are addressed. If you are not the original recipient or the person >>>> responsible for the delivering the email to the intended recipient, be >>>> advised that you have received this email in error, and that any use, >>>> dissemination, forwarding, printing or copying of this email is strictly >>>> prohibited. If you received this email in error, please delete it from your >>>> system without copying it, and notify the sender by reply email so that our >>>> address record can be corrected. >>>> >>>> >>>> >>>> ------------------------------ >>>> >>>> _______________________________________________ >>>> Histonet mailing list >>>> Histonet@lists.utsouthwestern.edu >>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>>> >>>> End of Histonet Digest, Vol 112, Issue 1 >>>> **************************************** >>>> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> > > From talulahgosh <@t> gmail.com Mon Mar 4 08:40:56 2013 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Mon Mar 4 08:41:01 2013 Subject: [Histonet] pH and temperature Message-ID: Hello! Can someone explain why pH is temperature dependent? Really, I'm just worried about pHing 4% paraformaldehyde at about 50 degrees Celsius. If I wait until it cools down to room temp, the para usually precipitates--I assume because the pH is off. I know I could measure the pH at room temperature, but I'm not making para anytime soon, and I just thought of this. By the way, the pH we are aiming for is 7.2 to 7.4 Emily "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." -Chuck Palahniuk, "Haunted" From mhale <@t> MiracaLS.com Mon Mar 4 08:43:20 2013 From: mhale <@t> MiracaLS.com (Hale, Meredith) Date: Mon Mar 4 08:43:25 2013 Subject: [Histonet] KY HT Poisitons Message-ID: <0E828EC51C7CC445A51E53F81B64E8C71B4DC5@s-irv-exchmb.PathologyPartners.intranet> Please no Recruiter calls Great opportunity for Histotechnician's in Crestview Hills, KY ! Tri-State Gastroenterology Associates is a multi-physician practice located in Northern Kentucky. Its mission is "To provide compassionate, high quality, cost-effective care to pateints with gastrointestinal problems" Looking for 2 Full Time HT/HTL's ( 32 hours a week is full time employement at this practice ) * Meet CLIA Grossing Requirements : CFR 493.1489, http://wwwn.cdc.gov/clia/regs/toc.aspx/ ,prior experience grossing GI specimens * Supervisor experience preferred * HT/HTL ASCP Certified * Experience with CLIA and CAP * Experience writing and maintaining policies and procedures * Prior laboratory start up experience is preferred * Ability to work independently Duties include: * Grossing * Embedding * Microtomy * Staining; routine and special stains only * Maintain supply orders and laboratory budget * Ability to be flexible and take on additional duties' as needed * Ability to work independently * Maintenance of laboratory for inspections * Maintenance of quality records Interested applicants should contact Meredith Hale; phone 214-596-2219 or through email mhale@miracals.com Meredith Hale HT (ASCP)cm Director External Sales Support Miraca Life Sciences 6655 North MacArthur Blvd. Irving , Texas 75039 Office: 214-596-2219 Cell: 469-648-8253 Fax: 1-866-688-3280 mhale@miracals.com> From dmccaig <@t> ckha.on.ca Mon Mar 4 10:07:56 2013 From: dmccaig <@t> ckha.on.ca (Diana McCaig) Date: Mon Mar 4 10:08:05 2013 Subject: [Histonet] standards for embedding Message-ID: I know this topic has been beaten to death in the past and do realize it is dependent on tissue orientation, quantity of pieces in a block, and experience. There are guidelines suggested by the College of Med Lab of Ontario in a document Oct 2008 that indicates the expected minimum daily range is Specimens with no orientation 16-38 seconds per specimen Specimens with orientation 21 to 52 seconds peer specimen Mixed cassettes 60-70 per hour I would like to know if this is monitored and what do you if your techs are unable to reach these recommendations, even after several years of experience. I realize these are minimum suggestions and should be achievable but what recommendations are suggested if a tech with over 10 years experience still averages close to 2 minutes a block. On average we may have 120-140 blocks a day and it takes 3.5-4 hours to embed. I have had ergonomic assessments done. They seem to maintain a constant level and will not strive for improvement. Any suggestions? Diana From rjbuesa <@t> yahoo.com Mon Mar 4 10:17:57 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Mar 4 10:18:05 2013 Subject: [Histonet] standards for embedding In-Reply-To: References: Message-ID: <1362413877.25340.YahooMailNeo@web163106.mail.bf1.yahoo.com> Ontario standards are above national and international averages and would be difficult to duplicate for the majority of labs. Your averages are more in accordance with the averages I mention. Please go to http://www.histosearch.com/rene.html to compare with the results of a survey I published. Ren? J. From: Diana McCaig To: "'histonet@lists.utsouthwestern.edu'" Sent: Monday, March 4, 2013 11:07 AM Subject: [Histonet] standards for embedding I know this topic has been beaten to death in the past and do realize it is dependent on tissue orientation, quantity of pieces in a block, and experience. There are guidelines suggested by the College of Med Lab of Ontario in a document Oct 2008 that indicates the expected minimum daily range is Specimens with no orientation? ? ? ? ? ? ? ? ? ? ? 16-38 seconds per specimen Specimens with orientation? ? ? ? ? ? ? ? ? ? ? ? ? ? 21 to 52 seconds peer specimen Mixed cassettes? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? 60-70 per hour I would like to know if this is monitored and what do you if your techs are unable to reach these recommendations, even after several years of experience.? I realize these are minimum suggestions and should be achievable but what recommendations are suggested if a tech with over 10 years experience still averages close to 2 minutes a block.? On average we may have 120-140 blocks a day and it takes 3.5-4 hours to embed.? I have had ergonomic assessments done. They seem to maintain a constant level and will not strive for improvement.? Any suggestions? Diana _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 <@t> earthlink.net Mon Mar 4 11:44:29 2013 From: relia1 <@t> earthlink.net (Pam Barker) Date: Mon Mar 4 11:44:32 2013 Subject: [Histonet] RELIA Solutions HOT JOB ALERT and a link to my blog post "Essential Tips for Working with a Recruiter" 3/4/2013 Message-ID: <01d501ce18ff$ec7523b0$c55f6b10$@earthlink.net> Hi Histonetters!! I hope your week is off to a great start!! I know mine is!! I have a couple of new opportunities that I want to share. Also I recently started a blog called Dream Job Diva here is the link to one of my posts if you think you might like to check it out. This has been without a doubt my most popular post which is why I chose it to share. I have even had other recruiting firms praise this post. - Enjoy. Here is the link: http://reliasolutionspambarker.wordpress.com/2012/07/28/esssential-tips-for- working-with-a-recruiter/ Here are the hot jobs I want to share with you! Histology Tech - East Texas - exciting opportunity to work in dermpath AND work with one of the top Mohs techs in the country! Histo Tech - Syracuse, NY - NYS license required Histotechnician - East of Columbus, OH IHC Specialist - Charlotte, NC Grossing Histotech - Charlotte, NC All of my clients offer excellent compensation, benefits and relocation assistance. These are full time permanent positions with stable secure labs. My clients are eager to interview and hire for these positions! If any of these opportunities are the right one for you RELIA can make it happen. If you do take the time to read my blog post I would love feedback. Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From BZIMMERM <@t> gru.edu Mon Mar 4 12:59:44 2013 From: BZIMMERM <@t> gru.edu (Zimmerman, Billie) Date: Mon Mar 4 12:59:54 2013 Subject: [Histonet] Jekyll Island, GA Message-ID: <7B3DEB32E69C034EACB479059C5DE3FF75930E@EX-MLB-03.ad.georgiahealth.edu> Come to Jekyll Island for the sunrise, sunsets, and CEU's!! Earn up to 15 CEU's with incredible speakers and a chance to network with your peers for the low all inclusive rate of $135. GSH is packing a punch for $135. GSH has planned an educational 40th anniversary celebration with fun, food, and time to visit the vendors. We have filled our block of rooms, but the symposium rate has been extended and is still available at Oceanside Inns and Suites. Also, if you love to ride bikes, bring them to the beach. I was impressed with the number of bike trails on the island. Go to our website and join the celebration. www.histosearch.com/gsh/ If you have any questions or concerns please contact one of our officers. Hope to see you there next month! Wanda K. Simons GSH President WS/bz Augusta State University and Georgia Health Sciences University have consolidated to become Georgia Regents University. Effective January 9, 2013, my email address has changed to BZIMMERM@gru.edu. Please update your address book to reflect this change. From TJohnson <@t> gnf.org Mon Mar 4 13:03:57 2013 From: TJohnson <@t> gnf.org (Teri Johnson) Date: Mon Mar 4 13:04:02 2013 Subject: [Histonet] Re: teeth sectioning Message-ID: <9F3CFEE76E51B64991C7485270890B404972C2EC@EX5.lj.gnf.org> This might or might not be helpful, but there was an article written by Janet Maas, Processing the Complete Canine Tooth. It is for paraffin sectioning. JOH, Number 3, Sept 2002, pp. 137-140(4) Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 From talulahgosh <@t> gmail.com Mon Mar 4 13:20:52 2013 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Mon Mar 4 13:21:01 2013 Subject: [Histonet] Re: pH and temperature In-Reply-To: <1C38BE2F76B4994C8DB9BCEB9195B23445F5B7D2@BN1PRD0712MB667.namprd07.prod.outlook.com> References: <1C38BE2F76B4994C8DB9BCEB9195B23445F5B7D2@BN1PRD0712MB667.namprd07.prod.outlook.com> Message-ID: Well, I found this on the site below: To make an accurate pH measurement you need to use temperature compensation. Exception is if you measure a pH of about 7. Which makes me feel better. Every other solution I pH is made at room temperature. I don't know why our para precipitates out--it might be because sometimes the water doesn't reach a high enough temperature before it's added, or the para solution cools down too much before the NaOH is added. It hasn't done it for a while though. Emily "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." -Chuck Palahniuk, "Haunted" On Mon, Mar 4, 2013 at 1:28 PM, Tamara Howard wrote: > It is pretty basic chemistry...Even has a website! > > http://www.all-about-ph.com/ph-versus-temperature.html > > From mkent <@t> dermpathlab.com Mon Mar 4 13:41:36 2013 From: mkent <@t> dermpathlab.com (Michael Kent) Date: Mon Mar 4 13:41:42 2013 Subject: [Histonet] Ventana HPV High/Low Replacements? In-Reply-To: <1362164943.49368.YahooMailNeo@web5814.biz.mail.ne1.yahoo.com> References: <1362164943.49368.YahooMailNeo@web5814.biz.mail.ne1.yahoo.com> Message-ID: <27EB7FFB1A15F549B17F79B1A856C70BC00BFB@dlcs-sbs1.DPLCS.intra> Hi Jennifer, I'd recommend looking at this study and comments on biomarkers in HPV: The Lower Anogenital Squamous Terminology Standardization Project for HPV-Associated Lesions: Background and Consensus Recommendations from the College of American Pathologists and the American Society for Colposcopy and Cervical Pathology Arch Pathol Lab Med-Vol 136, October 2012 The CAP-ASCCP LAST Project--Darragh et al Regards, Mike Michael Kent, MS, PhD www.dermpathlab.com Confidentiality Notice: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. Thank you. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer Masuzumi Sent: Friday, March 01, 2013 2:09 PM To: Histonet Subject: [Histonet] Ventana HPV High/Low Replacements? Hello everyone, My lab is interested in starting?HPV?High and Low Risk ISH testing, but it seems like we just missed the boat on it. We have Benchmark XTs, but Ventana discontinued their HPV probes sometime around last December, and they don't have an alternative at this time. After talking with nearby labs that are currently using stockpiles of Ventana probes, they told me the following courses of action they were planning on using when their stock runs out: -One lab is thinking of doing HPV by PCR -Another is thinking of switching to Dako probes After discussing the possibility of Dako probes with Ventana and Dako, neither could recommend using them together because each side was unfamiliar with the other's reagents or system, though both said it might be possible. Is anybody in a similar situation, looking for a replacement for the Ventana HPV High/Low Risk probes? Any insight on possibilities to pursue would be much appreciated! Sincerely, ? Jennifer Masuzumi Sterling Pathology Seal Beach, CA jennifer.masuzumi@sterlingpath.com 562-799-8900 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rebecca.Riesen <@t> hma.com Mon Mar 4 14:33:40 2013 From: Rebecca.Riesen <@t> hma.com (Riesen, Rebecca) Date: Mon Mar 4 14:33:47 2013 Subject: [Histonet] QC Slide retention Message-ID: How long do you folks retain you Daily QC slides? Is it the same as the "diagnostic" slides? I know the correct length of time for "diagnostic" slides in my particular area, but I wasn't sure if the same timeline applies to the actual daily H&E or PAP QC slides. Is the retention of the actual "documentation" that I have of the quality of these stains each day sufficient? I looked thru the archives, but could only find the previous discussions on "Diagnostic" slides. Thanks Histonetters!! Rebecca Riesen, Histology Supervisor, PRMC, Naples, FL From lcolbert <@t> pathmdlabs.com Mon Mar 4 14:41:10 2013 From: lcolbert <@t> pathmdlabs.com (Laurie Colbert) Date: Mon Mar 4 14:47:22 2013 Subject: [Histonet] Sponges and Processing Message-ID: <12ECD7346266D74691EC2BFC75285E451CC02291@BFL323E10.pathmdlabs.local> If there is anyone out there that uses the blue sponges for biopsy specimens, would you please contact me offline? I would like to know what your processing times are. Thanks! Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab From b-frederick <@t> northwestern.edu Mon Mar 4 14:45:49 2013 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Mon Mar 4 15:01:02 2013 Subject: [Histonet] RE: QC Slide retention In-Reply-To: References: Message-ID: <62C639732D3F274DACED033EBDF6ADAF20D0A85D@evcspmbx2.ads.northwestern.edu> We keep ours for 2 years- kind of like those lovely QC sheets for CAP. Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Riesen, Rebecca Sent: Monday, March 04, 2013 2:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] QC Slide retention How long do you folks retain you Daily QC slides? Is it the same as the "diagnostic" slides? I know the correct length of time for "diagnostic" slides in my particular area, but I wasn't sure if the same timeline applies to the actual daily H&E or PAP QC slides. Is the retention of the actual "documentation" that I have of the quality of these stains each day sufficient? I looked thru the archives, but could only find the previous discussions on "Diagnostic" slides. Thanks Histonetters!! Rebecca Riesen, Histology Supervisor, PRMC, Naples, FL _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Joyce.Weems <@t> emoryhealthcare.org Mon Mar 4 15:18:14 2013 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Mon Mar 4 15:18:26 2013 Subject: [Histonet] RE: QC Slide retention In-Reply-To: <62C639732D3F274DACED033EBDF6ADAF20D0A85D@evcspmbx2.ads.northwestern.edu> References: <62C639732D3F274DACED033EBDF6ADAF20D0A85D@evcspmbx2.ads.northwestern.edu> Message-ID: We do the same. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Monday, March 04, 2013 3:46 PM To: Riesen, Rebecca; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: QC Slide retention We keep ours for 2 years- kind of like those lovely QC sheets for CAP. Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Riesen, Rebecca Sent: Monday, March 04, 2013 2:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] QC Slide retention How long do you folks retain you Daily QC slides? Is it the same as the "diagnostic" slides? I know the correct length of time for "diagnostic" slides in my particular area, but I wasn't sure if the same timeline applies to the actual daily H&E or PAP QC slides. Is the retention of the actual "documentation" that I have of the quality of these stains each day sufficient? I looked thru the archives, but could only find the previous discussions on "Diagnostic" slides. Thanks Histonetters!! Rebecca Riesen, Histology Supervisor, PRMC, Naples, FL _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From brannon <@t> alliedsearchpartners.com Mon Mar 4 15:37:18 2013 From: brannon <@t> alliedsearchpartners.com (Brannon Owens) Date: Mon Mar 4 15:37:30 2013 Subject: [Histonet] Per Diem opportunity near Port Chester, NY Message-ID: Allied Search Partners is currently working with a large organization with leading edge technologies to find a qualified Histology professional. Location: White Plains/Port Chester, NY area Position title: Histotechnician or Histotechnologist Schedule: Sunday 5am-1:30pm Email brannon@alliesearchpartners.com for a full job description. NY lab license is required. Brannon Owens Recruitment Manager Allied Search Partners From SHUNTER <@t> beaumont.edu Mon Mar 4 16:05:18 2013 From: SHUNTER <@t> beaumont.edu (Sue Hunter) Date: Mon Mar 4 16:05:26 2013 Subject: [Histonet] RE: Antibody IDH1 In-Reply-To: <7B3DEB32E69C034EACB479059C5DE3FF75726C@EX-MLB-03.ad.georgiahealth.edu> References: <7B3DEB32E69C034EACB479059C5DE3FF75726C@EX-MLB-03.ad.georgiahealth.edu> Message-ID: We use Dianova's antibody at a 1:100 dilution on the Ultra. CC1 cell conditioning for 40 minutes, antibody for 32 minutes - no heat. We use Optiview detection system. No amp. Make sure you are using the correct type of brain tissue for a control - most oligodendrogliomas should be positive, altho there are a few with other mutations. Remember that this antibody only picks up the R132H point mutation. This occurs in about 70% of oligos so it is possible that you happened to pick one that was positive for one of the other mutations. Perhaps try a few different patient tissues. Sue Sue Hunter, Supervisor Advanced Diagnostics Beaumont Health System Royal Oak MI 248-898-5146 shunter@beaumont.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Zimmerman, Billie Sent: Friday, February 22, 2013 4:21 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Antibody IDH1 Does anyone perform this antibody?? We can't get it to work on the Ventana Ultra. PhenoPath in Seattle performs it on the Dako autostainer using a small size polymer detection kit. I would appreciate any input. Thanks, Billie Zimmerman Augusta State University and Georgia Health Sciences University have consolidated to become Georgia Regents University. Effective January 9, 2013, my email address has changed to BZIMMERM@gru.edu. Please update your address book to reflect this change. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tkngflght <@t> yahoo.com Mon Mar 4 16:32:30 2013 From: tkngflght <@t> yahoo.com (Cheryl) Date: Mon Mar 4 16:32:33 2013 Subject: [Histonet] SOP numbering for whole lab Message-ID: <1362436350.93849.YahooMailClassic@web161905.mail.bf1.yahoo.com> Help?? What kind of formula do you use when numbering and coding your SOPs for the lab?? Is there an easy way to keep them sorted and in line with all the regulatory bodies to help on inspection days? ? Any suggestions welcome--we're starting from scratch. ? Cheryl ? ? From andreahooper <@t> rocketmail.com Mon Mar 4 17:35:19 2013 From: andreahooper <@t> rocketmail.com (Andrea Hooper) Date: Mon Mar 4 17:35:32 2013 Subject: [Histonet] Back processing Histogel Message-ID: <14FF0544-3E08-4DA8-AAAC-ABFD8BAC392E@rocketmail.com> Has anyone back processed something that was both Histogel and FFPE embedded? If so, any hints or problems? Andrea From owensc500 <@t> gmail.com Mon Mar 4 18:57:51 2013 From: owensc500 <@t> gmail.com (Clarence Owens) Date: Mon Mar 4 18:57:58 2013 Subject: [Histonet] Paraffin block archival storage Message-ID: <41E471D1-99B1-4C21-B2C0-3BE0BDC5F47E@gmail.com> Does anyone know if there is a CAP or board of health regulation on storing archival paraffin blocks in a walk in morgue cooler. Currently we are storing our archival paraffin blocks in the morgue cooler for the ten year retention CAP regulation. The blocks are at a controlled temperature where the integrity of the blocks are not compromised. The blocks are stored in their block filing cabinet in one part of the cooler. Is there a problem with having the blocks stored in this area. Lastly the cooler is under lock and key and the key has to be signed out as well. This also provide a security aspect for the blocks and monitors who enters in and out of this secured area. Thanks for your help. Clarence Owens, HT (ASCP) Lawrence General Hospital Lawrence, MA 01842 From ratliffjack <@t> hotmail.com Tue Mar 5 10:16:13 2013 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Tue Mar 5 10:16:21 2013 Subject: [Histonet] teeth sectioning In-Reply-To: <513489D7.2020804@pigsqq.org> References: <1E916435-F342-4738-BDEC-1BAF7A0FB6C2@hotmail.com>, <513489D7.2020804@pigsqq.org> Message-ID: VGhhbmtzIGZvciB5b3VyIG1lc3NhZ2UgV2F5bmUuIEkgd2lsbCBkZWZpbml0ZWx5IGZvbGxvdyB1 cCB3aXRoIHlvdSB1cG9uIG15IHJldHVybiEgUGxlYXNlIGxldCBtZSBrbm93IGlmIHRoZXJlIGlz IGFueXRoaW5nIGVsc2UgdGhhdCBpbnRlcmVzdCB5b3Ugd2l0aCByZWdhcmRzIHRvIEhhcmQgVGlz c3VlIHNwZWNpbWVuIHR5cGVzLiBJIHNwZWNpZmljYWxseSB3b3JrIHdpdGggdGhlIGhpc3RvbG9n eSByZWxhdGVkIHRvIGJvbmUsIGJpb21hdGVyaWFscyBhbmQgbWVkaWNhbCBkZXZpY2UgaW1wbGFu dHMuIEluIGZhY3QsIEkgd2lsbCBiZSBwcmVzZW50aW5nIG9uIHRoZXNlIHRvcGljcyBhdCBzZXZl cmFsIGhpc3RvbG9neSBtZWV0aW5ncyBoZXJlIGluIHRoZSBVLlMuIHRocm91Z2hvdXQgdGhlIHll 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X19fX19fX19fDQo+ID4+Pj4gSGlzdG9uZXQgbWFpbGluZyBsaXN0DQo+ID4+Pj4gSGlzdG9uZXRA bGlzdHMudXRzb3V0aHdlc3Rlcm4uZWR1DQo+ID4+Pj4gaHR0cDovL2xpc3RzLnV0c291dGh3ZXN0 ZXJuLmVkdS9tYWlsbWFuL2xpc3RpbmZvL2hpc3RvbmV0DQo+ID4+Pj4NCj4gPj4+PiBFbmQgb2Yg SGlzdG9uZXQgRGlnZXN0LCBWb2wgMTEyLCBJc3N1ZSAxDQo+ID4+Pj4gKioqKioqKioqKioqKioq KioqKioqKioqKioqKioqKioqKioqKioqKg0KPiA+Pj4+ICAgICAgICAgIA0KPiA+PiBfX19fX19f X19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fXw0KPiA+PiBIaXN0b25ldCBt YWlsaW5nIGxpc3QNCj4gPj4gSGlzdG9uZXRAbGlzdHMudXRzb3V0aHdlc3Rlcm4uZWR1DQo+ID4+ IGh0dHA6Ly9saXN0cy51dHNvdXRod2VzdGVybi5lZHUvbWFpbG1hbi9saXN0aW5mby9oaXN0b25l dA0KPiA+Pg0KPiA+PiAgICAgIA0KPiA+DQo+ID4gICAgDQo+IA0KIAkJIAkgICAJCSAg From lcolbert <@t> pathmdlabs.com Tue Mar 5 12:00:15 2013 From: lcolbert <@t> pathmdlabs.com (Laurie Colbert) Date: Tue Mar 5 12:06:25 2013 Subject: [Histonet] RE: QC Slide retention In-Reply-To: References: Message-ID: <12ECD7346266D74691EC2BFC75285E451CC02389@BFL323E10.pathmdlabs.local> We keep ours long enough to fill up a 100-slide box and then dump the oldest ones. We do have a separate chart where we document daily that the H&E slide is checked, and this is kept for at least two years along with our other QC (temps, processor rotation, etc) charts. Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Riesen, Rebecca Sent: Monday, March 04, 2013 12:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] QC Slide retention How long do you folks retain you Daily QC slides? Is it the same as the "diagnostic" slides? I know the correct length of time for "diagnostic" slides in my particular area, but I wasn't sure if the same timeline applies to the actual daily H&E or PAP QC slides. Is the retention of the actual "documentation" that I have of the quality of these stains each day sufficient? I looked thru the archives, but could only find the previous discussions on "Diagnostic" slides. Thanks Histonetters!! Rebecca Riesen, Histology Supervisor, PRMC, Naples, FL _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HParker <@t> Skaggs.Net Tue Mar 5 12:09:57 2013 From: HParker <@t> Skaggs.Net (Parker, Helayne) Date: Tue Mar 5 12:10:02 2013 Subject: [Histonet] Pregnancy In-Reply-To: References: Message-ID: <930EB2E8DF68C544873EDD2A3D5F506007D0300D42@email1.skaggs.net> Hi Gang, Thought I would ask some people with some more experience than I in this. One of our techs is pregnant- Yay !!! She is very early - about 5 weeks. My Lab Director wants her to start wearing a mask due to the chemicals. So we need to ask what sort of precautions need to take place during the pregnancy etc. We also coverslip by hand here out of xylene etc. I can not remember ever working with any pregnant HTs in the past so I honestly do not know. Please carbon copy replies to JAScholefield@skaggs.net as well. We predominately work with 10% formalin, reagent grade alcohols, paraplast, xylenes, clear-rite 3, Decal I and II (from Surgipath), cytofixative and some routine special stain items. Basically the typical myriad of stuff a very small routine histo lab would have (no immunos).... Thanks and bunch for your help ! Sincerely, Helayne Parker, H.T. (ASCP) Pathology Section Head Cox Medical Center Branson P.O. Box 650, Branson, MO 65615 Phone: 417-335-7254 Fax: 417-335-7127 Email: hparker@skaggs.net Web: www.coxhealth.com/branson -------------- next part -------------- CoxHealth ? ranked one of Missouri's Best Hospitals by U.S. News & World Report COXHEALTH CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From jstaruk <@t> masshistology.com Tue Mar 5 12:27:29 2013 From: jstaruk <@t> masshistology.com (JStaruk) Date: Tue Mar 5 12:27:32 2013 Subject: [Histonet] Pregnancy In-Reply-To: <930EB2E8DF68C544873EDD2A3D5F506007D0300D42@email1.skaggs.net> References: <930EB2E8DF68C544873EDD2A3D5F506007D0300D42@email1.skaggs.net> Message-ID: <006801ce19cf$19100cd0$4b302670$@masshistology.com> We have a form for all pregnant women (and her doctor) to sign. The form lists the chemicals she will be exposed to and the potential risks of each are outlined. The form also reminds the employee that she should continue to use universal precautions when working around these chemicals. I like to have her doctor acknowledge that he/she is aware of the employee's work environment and that the employee has her doctor's OK to continue working here. I feel that having both sign this form protects us from any possible liability. _______________________ James E.?Staruk HT(ASCP) ?www.masshistology.com ?? www.nehorselabs.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Parker, Helayne Sent: Tuesday, March 05, 2013 1:10 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Pregnancy Hi Gang, Thought I would ask some people with some more experience than I in this. One of our techs is pregnant- Yay !!! She is very early - about 5 weeks. My Lab Director wants her to start wearing a mask due to the chemicals. So we need to ask what sort of precautions need to take place during the pregnancy etc. We also coverslip by hand here out of xylene etc. I can not remember ever working with any pregnant HTs in the past so I honestly do not know. Please carbon copy replies to JAScholefield@skaggs.net as well. We predominately work with 10% formalin, reagent grade alcohols, paraplast, xylenes, clear-rite 3, Decal I and II (from Surgipath), cytofixative and some routine special stain items. Basically the typical myriad of stuff a very small routine histo lab would have (no immunos).... Thanks and bunch for your help ! Sincerely, Helayne Parker, H.T. (ASCP) Pathology Section Head Cox Medical Center Branson P.O. Box 650, Branson, MO 65615 Phone: 417-335-7254 Fax: 417-335-7127 Email: hparker@skaggs.net Web: www.coxhealth.com/branson From rjbuesa <@t> yahoo.com Tue Mar 5 12:29:58 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Mar 5 12:30:03 2013 Subject: [Histonet] Pregnancy In-Reply-To: <930EB2E8DF68C544873EDD2A3D5F506007D0300D42@email1.skaggs.net> References: <930EB2E8DF68C544873EDD2A3D5F506007D0300D42@email1.skaggs.net> Message-ID: <1362508198.71405.YahooMailNeo@web163101.mail.bf1.yahoo.com> Using carbon masks is acceptable but not the best solution which is assigning tasks not requiring working with xylene which is EXTREMELY dangerous. Please go to http://www.histosearch.com/rene.html ?and read the article about xylene substitution where I discuss precisely this issue. By the way, in that same website you can learn how to dewax and coverslip WITHOUT xylene. Ren? J. From: "Parker, Helayne" To: "'histonet@lists.utsouthwestern.edu'" Sent: Tuesday, March 5, 2013 1:09 PM Subject: [Histonet] Pregnancy Hi Gang, ? Thought I would ask some people with some more experience than I in this.? One of our techs is pregnant-? Yay !!!? She is very early - about 5 weeks.? My Lab Director wants her to start wearing a mask due to the chemicals.? So we need to ask what sort of precautions need to take place during the pregnancy etc.? We also coverslip by hand here out of xylene etc.? I can not remember ever working with any pregnant HTs in the past so I honestly do not know.? Please carbon copy replies to JAScholefield@skaggs.net as well.? We predominately work with 10% formalin, reagent grade alcohols, paraplast, xylenes, clear-rite 3, Decal I and II (from Surgipath), cytofixative and some routine special stain items. Basically the typical myriad of stuff a very small routine histo lab would have (no immunos).... Thanks and bunch for your help ! Sincerely, Helayne Parker, H.T. (ASCP) Pathology Section Head Cox Medical Center Branson P.O. Box 650, Branson, MO 65615 Phone:? 417-335-7254 Fax:? 417-335-7127 Email:? hparker@skaggs.net Web:? www.coxhealth.com/branson CoxHealth ? ranked one of Missouri's Best Hospitals by U.S. News & World Report COXHEALTH CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law.? Any unauthorized review, use, disclosure or distribution is prohibited.? If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Mar 5 12:33:41 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Mar 5 12:33:45 2013 Subject: [Histonet] Pregnancy In-Reply-To: <006801ce19cf$19100cd0$4b302670$@masshistology.com> References: <930EB2E8DF68C544873EDD2A3D5F506007D0300D42@email1.skaggs.net> <006801ce19cf$19100cd0$4b302670$@masshistology.com> Message-ID: <1362508421.39777.YahooMailNeo@web163106.mail.bf1.yahoo.com> Protecting the employer for a possible liability (and law suit after the fact) cannot cover the compensation for a miscarriage or a permanently disabled child. The issue is not protecting the employer, but protecting the employee and her child. I am sorry, but that is how I see it! Ren? J. From: JStaruk To: "'Parker, Helayne'" ; histonet@lists.utsouthwestern.edu Sent: Tuesday, March 5, 2013 1:27 PM Subject: RE: [Histonet] Pregnancy We have a form for all pregnant women (and her doctor) to sign.? The form lists the chemicals she will be exposed to and the potential risks of each are outlined.? The form also reminds the employee that she should continue to use universal precautions when working around these chemicals.? I like to have her doctor acknowledge that he/she is aware of the employee's work environment and that the employee has her doctor's OK to continue working here.? I feel that having both sign this form protects us from any possible liability. _______________________ James E.?Staruk HT(ASCP) ?http://www.masshistology.com/ ?? http://www.nehorselabs.com/ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Parker, Helayne Sent: Tuesday, March 05, 2013 1:10 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Pregnancy Hi Gang, ? Thought I would ask some people with some more experience than I in this. One of our techs is pregnant-? Yay !!!? She is very early - about 5 weeks. My Lab Director wants her to start wearing a mask due to the chemicals.? So we need to ask what sort of precautions need to take place during the pregnancy etc.? We also coverslip by hand here out of xylene etc.? I can not remember ever working with any pregnant HTs in the past so I honestly do not know.? Please carbon copy replies to JAScholefield@skaggs.net as well.? We predominately work with 10% formalin, reagent grade alcohols, paraplast, xylenes, clear-rite 3, Decal I and II (from Surgipath), cytofixative and some routine special stain items. Basically the typical myriad of stuff a very small routine histo lab would have (no immunos).... Thanks and bunch for your help ! Sincerely, Helayne Parker, H.T. (ASCP) Pathology Section Head Cox Medical Center Branson P.O. Box 650, Branson, MO 65615 Phone:? 417-335-7254 Fax:? 417-335-7127 Email:? hparker@skaggs.net Web:? www.coxhealth.com/branson _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Tue Mar 5 13:00:16 2013 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Tue Mar 5 13:00:20 2013 Subject: [Histonet] RE: Pregnancy In-Reply-To: <930EB2E8DF68C544873EDD2A3D5F506007D0300D42@email1.skaggs.net> References: <930EB2E8DF68C544873EDD2A3D5F506007D0300D42@email1.skaggs.net> Message-ID: <14E2C6176416974295479C64A11CB9AE016411AC2793@SBS2K8.premierlab.local> Helayne Since OSHA does not have any guidelines with respects to xylene and formaldehyde in pregnancy (they do mention pregnancy in both standards, but no PEL's, etc.) her restrictions need to come from her OB/GYN physician. This was told to me by an OSHA industrial hygienist that was in our lab. What is a mask going to do? Not unless you are referring to one of those 3M respirators 3M 8247 - Nuisance level organic vapor relief. If your chemical monitoring results are below the OSHA standard then you could bring these respirators in as voluntary use. You need to have the appropriate safety policies and training associated with that. I have those in place so if you need help with that I can help. If for any reason even though you are below the PEL's and you want to bring in a tight fit respirators then you have to have a full blown respiratory protection program. Your responsibility as an employer is to perform a written hazard assessment of the job (based on sampling results) the employees can then be offered filtering face pieces and other proper PPE. Just my two cents. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Laboratory Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 Work (303) 682-3949 Fax (303) 682-9060 Cell (303) 881-0763 liz@premierlab.com www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Parker, Helayne Sent: Tuesday, March 05, 2013 10:10 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Pregnancy Hi Gang, Thought I would ask some people with some more experience than I in this. One of our techs is pregnant- Yay !!! She is very early - about 5 weeks. My Lab Director wants her to start wearing a mask due to the chemicals. So we need to ask what sort of precautions need to take place during the pregnancy etc. We also coverslip by hand here out of xylene etc. I can not remember ever working with any pregnant HTs in the past so I honestly do not know. Please carbon copy replies to JAScholefield@skaggs.net as well. We predominately work with 10% formalin, reagent grade alcohols, paraplast, xylenes, clear-rite 3, Decal I and II (from Surgipath), cytofixative and some routine special stain items. Basically the typical myriad of stuff a very small routine histo lab would have (no immunos).... Thanks and bunch for your help ! Sincerely, Helayne Parker, H.T. (ASCP) Pathology Section Head Cox Medical Center Branson P.O. Box 650, Branson, MO 65615 Phone: 417-335-7254 Fax: 417-335-7127 Email: hparker@skaggs.net Web: www.coxhealth.com/branson From b-frederick <@t> northwestern.edu Tue Mar 5 13:04:10 2013 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Tue Mar 5 13:04:16 2013 Subject: [Histonet] Pregnancy In-Reply-To: <1362508421.39777.YahooMailNeo@web163106.mail.bf1.yahoo.com> References: <930EB2E8DF68C544873EDD2A3D5F506007D0300D42@email1.skaggs.net> <006801ce19cf$19100cd0$4b302670$@masshistology.com> <1362508421.39777.YahooMailNeo@web163106.mail.bf1.yahoo.com> Message-ID: <62C639732D3F274DACED033EBDF6ADAF20D0A9CD@evcspmbx2.ads.northwestern.edu> We don't even let them near the stainers, processors or coverslippers. Those I've seen that are expecting can't deal with the smell anyway. It's a teratogen. I wouldn't risk it either. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, March 05, 2013 12:34 PM To: JStaruk; 'Parker, Helayne'; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Pregnancy Protecting the employer for a possible liability (and law suit after the fact) cannot cover the compensation for a miscarriage or a permanently disabled child. The issue is not protecting the employer, but protecting the employee and her child. I am sorry, but that is how I see it! Ren? J. From: JStaruk To: "'Parker, Helayne'" ; histonet@lists.utsouthwestern.edu Sent: Tuesday, March 5, 2013 1:27 PM Subject: RE: [Histonet] Pregnancy We have a form for all pregnant women (and her doctor) to sign.? The form lists the chemicals she will be exposed to and the potential risks of each are outlined.? The form also reminds the employee that she should continue to use universal precautions when working around these chemicals.? I like to have her doctor acknowledge that he/she is aware of the employee's work environment and that the employee has her doctor's OK to continue working here.? I feel that having both sign this form protects us from any possible liability. _______________________ James E.?Staruk HT(ASCP) ?http://www.masshistology.com/ ?? http://www.nehorselabs.com/ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Parker, Helayne Sent: Tuesday, March 05, 2013 1:10 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Pregnancy Hi Gang, ? Thought I would ask some people with some more experience than I in this. One of our techs is pregnant-? Yay !!!? She is very early - about 5 weeks. My Lab Director wants her to start wearing a mask due to the chemicals.? So we need to ask what sort of precautions need to take place during the pregnancy etc.? We also coverslip by hand here out of xylene etc.? I can not remember ever working with any pregnant HTs in the past so I honestly do not know.? Please carbon copy replies to JAScholefield@skaggs.net as well.? We predominately work with 10% formalin, reagent grade alcohols, paraplast, xylenes, clear-rite 3, Decal I and II (from Surgipath), cytofixative and some routine special stain items. Basically the typical myriad of stuff a very small routine histo lab would have (no immunos).... Thanks and bunch for your help ! Sincerely, Helayne Parker, H.T. (ASCP) Pathology Section Head Cox Medical Center Branson P.O. Box 650, Branson, MO 65615 Phone:? 417-335-7254 Fax:? 417-335-7127 Email:? hparker@skaggs.net Web:? www.coxhealth.com/branson _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kiran_g <@t> sbcglobal.net Tue Mar 5 13:44:02 2013 From: kiran_g <@t> sbcglobal.net (Kiranjit Grewal) Date: Tue Mar 5 13:44:11 2013 Subject: [Histonet] Leak proof transport containers Message-ID: Dear Histonetters, Does anyone in histology world know about leak proof containers that can hold tissue processing baskets for transporting cassettes in formalin? We had no luck so far. Any help with this will be much appreciated! Thank you, Kiran Sent from my iPhone From HParker <@t> Skaggs.Net Tue Mar 5 14:31:45 2013 From: HParker <@t> Skaggs.Net (Parker, Helayne) Date: Tue Mar 5 14:31:50 2013 Subject: [Histonet] Pregnancy (more) In-Reply-To: <929ae941-6de3-4859-993f-888445bcef14@email1.skaggs.net> References: <929ae941-6de3-4859-993f-888445bcef14@email1.skaggs.net> Message-ID: <930EB2E8DF68C544873EDD2A3D5F506007D0300D52@email1.skaggs.net> Hi, Thanks all for all the info. We have a plan in place for the time being. She will only be embedding and sectioning slides at a station that we set up in the clinical lab. I will be doing all the rest of the stuff. Helayne Parker, H.T. (ASCP) Pathology Section Head Cox Medical Center Branson P.O. Box 650, Branson, MO 65615 Phone: 417-335-7254 Fax: 417-335-7127 Email: hparker@skaggs.net Web: www.coxhealth.com/branson -------------- next part -------------- CoxHealth ? ranked one of Missouri's Best Hospitals by U.S. News & World Report COXHEALTH CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From Karen.Heckford <@t> DignityHealth.org Tue Mar 5 13:54:51 2013 From: Karen.Heckford <@t> DignityHealth.org (Heckford, Karen - SMMC-SF) Date: Tue Mar 5 16:34:12 2013 Subject: [Histonet] RE: Pregnancy In-Reply-To: <930EB2E8DF68C544873EDD2A3D5F506007D0300D42@email1.skaggs.net> References: <930EB2E8DF68C544873EDD2A3D5F506007D0300D42@email1.skaggs.net> Message-ID: I was working in a Histology Lab when I first got pregnant. I told my doctor about everything I was exposed to in the lab. He actually opted on putting me on medical leave (workman's comp). I did this with both of my pregnancies. Not in less you can find her a job that she is not exposed. I would not risk exposure. Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Parker, Helayne Sent: Tuesday, March 05, 2013 10:10 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Pregnancy Hi Gang, Thought I would ask some people with some more experience than I in this. One of our techs is pregnant- Yay !!! She is very early - about 5 weeks. My Lab Director wants her to start wearing a mask due to the chemicals. So we need to ask what sort of precautions need to take place during the pregnancy etc. We also coverslip by hand here out of xylene etc. I can not remember ever working with any pregnant HTs in the past so I honestly do not know. Please carbon copy replies to JAScholefield@skaggs.net as well. We predominately work with 10% formalin, reagent grade alcohols, paraplast, xylenes, clear-rite 3, Decal I and II (from Surgipath), cytofixative and some routine special stain items. Basically the typical myriad of stuff a very small routine histo lab would have (no immunos).... Thanks and bunch for your help ! Sincerely, Helayne Parker, H.T. (ASCP) Pathology Section Head Cox Medical Center Branson P.O. Box 650, Branson, MO 65615 Phone: 417-335-7254 Fax: 417-335-7127 Email: hparker@skaggs.net Web: www.coxhealth.com/branson From sugartop2000 <@t> gmail.com Tue Mar 5 16:51:18 2013 From: sugartop2000 <@t> gmail.com (Sirena Hudgins) Date: Tue Mar 5 16:51:23 2013 Subject: [Histonet] Pregnancy In-Reply-To: <930EB2E8DF68C544873EDD2A3D5F506007D0300D42@email1.skaggs.net> References: <930EB2E8DF68C544873EDD2A3D5F506007D0300D42@email1.skaggs.net> Message-ID: We have had several healthy pregnancys in our lab. I was not as fortunate, at a previous lab, I worked in. Never let anyone tell your tech THAT THEY DID STAINING COVERSLIPPING AND WET TRIMMING and their baby was fine. Never make them feel guilty or pressured to work with chemicals. Instead insist that they are not even allowed in those areas of the lab, this includes unloading the processor. Be concerned for your employees health and well being, and the life that is being formed. On Mar 5, 2013 1:10 PM, "Parker, Helayne" wrote: > > Hi Gang, > Thought I would ask some people with some more experience than I in this. One of our techs is pregnant- Yay !!! She is very early - about 5 weeks. My Lab Director wants her to start wearing a mask due to the chemicals. So we need to ask what sort of precautions need to take place during the pregnancy etc. We also coverslip by hand here out of xylene etc. I can not remember ever working with any pregnant HTs in the past so I honestly do not know. Please carbon copy replies to JAScholefield@skaggs.net as well. We predominately work with 10% formalin, reagent grade alcohols, paraplast, xylenes, clear-rite 3, Decal I and II (from Surgipath), cytofixative and some routine special stain items. Basically the typical myriad of stuff a very small routine histo lab would have (no immunos).... > > Thanks and bunch for your help ! > > > Sincerely, > > Helayne Parker, H.T. (ASCP) > Pathology Section Head > Cox Medical Center Branson > P.O. Box 650, Branson, MO 65615 > Phone: 417-335-7254 > Fax: 417-335-7127 > Email: hparker@skaggs.net > Web: www.coxhealth.com/branson > > > CoxHealth ? ranked one of Missouri's Best Hospitals by U.S. News & World Report > COXHEALTH CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From amosbrooks <@t> gmail.com Tue Mar 5 17:12:22 2013 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Tue Mar 5 17:12:27 2013 Subject: Subject: Re: [Histonet] standards for embedding In-Reply-To: References: Message-ID: Hi, Keeping records on how many cassettes were embedded and how long it took is not a bad idea. That having been said, there are many reasons that it could take more or less time than those given. It is also worth considering how long it takes to compare these numbers to some vague goals set in other facilities. If the times are always long, it would be immediately apparent and perhaps it's worth discussing with someone. If it isn't obvious that there is a problem then it is probably worth looking into how much time is spent trying to find one. Amos Brooks From liz <@t> premierlab.com Tue Mar 5 17:20:52 2013 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Tue Mar 5 17:21:05 2013 Subject: [Histonet] RE: Pregnancy In-Reply-To: References: <930EB2E8DF68C544873EDD2A3D5F506007D0300D42@email1.skaggs.net> Message-ID: <14E2C6176416974295479C64A11CB9AE016411AC279D@SBS2K8.premierlab.local> Karen Are you sure it was workman's comp. Workman's comp applies to individuals injured on the job. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Laboratory Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 Work (303) 682-3949 Fax (303) 682-9060 Cell (303) 881-0763 liz@premierlab.com www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Heckford, Karen - SMMC-SF Sent: Tuesday, March 05, 2013 11:55 AM To: 'Parker, Helayne'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Pregnancy I was working in a Histology Lab when I first got pregnant. I told my doctor about everything I was exposed to in the lab. He actually opted on putting me on medical leave (workman's comp). I did this with both of my pregnancies. Not in less you can find her a job that she is not exposed. I would not risk exposure. Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Parker, Helayne Sent: Tuesday, March 05, 2013 10:10 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Pregnancy Hi Gang, Thought I would ask some people with some more experience than I in this. One of our techs is pregnant- Yay !!! She is very early - about 5 weeks. My Lab Director wants her to start wearing a mask due to the chemicals. So we need to ask what sort of precautions need to take place during the pregnancy etc. We also coverslip by hand here out of xylene etc. I can not remember ever working with any pregnant HTs in the past so I honestly do not know. Please carbon copy replies to JAScholefield@skaggs.net as well. We predominately work with 10% formalin, reagent grade alcohols, paraplast, xylenes, clear-rite 3, Decal I and II (from Surgipath), cytofixative and some routine special stain items. Basically the typical myriad of stuff a very small routine histo lab would have (no immunos).... Thanks and bunch for your help ! Sincerely, Helayne Parker, H.T. (ASCP) Pathology Section Head Cox Medical Center Branson P.O. Box 650, Branson, MO 65615 Phone: 417-335-7254 Fax: 417-335-7127 Email: hparker@skaggs.net Web: www.coxhealth.com/branson _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wilson6848 <@t> yahoo.com Tue Mar 5 17:21:20 2013 From: wilson6848 <@t> yahoo.com (Wilson A) Date: Tue Mar 5 17:21:23 2013 Subject: [Histonet] Kappa/Lambda ISH Message-ID: <1362525680.80549.YahooMailNeo@web125404.mail.ne1.yahoo.com> ? ??? Hi, ??????? Please I am wondering whether you guys run controls with your Kappa/Lambda ISH. ? ???? Thanks, ???? Wilson From Dingersoll <@t> aplaboratories.com Tue Mar 5 17:26:23 2013 From: Dingersoll <@t> aplaboratories.com (Dingersoll@aplaboratories.com) Date: Tue Mar 5 17:26:29 2013 Subject: [Histonet] [FWD: Opportunity in Charleston, SC] Message-ID: <20130305162623.073ecbdb5144cf8a05e574ee22bfb11a.ce13c3a91c.wbe@email17.secureserver.net> dingersoll@aplaboratories.com -------- Original Message -------- Subject: From: <[1]Dingersoll@aplaboratories.com> Date: Tue, March To: [2]histonet-request@lists.utsouthwestern.edu AP Laboratories, LLC, a rapidly in Charleston, SC, is accepting appl histology technicians/technologists and gro offer an excellent compensation and benefit pac employees. Please email your resume to [3]dingersoll@a plaboratories.com or visit our website at [4]www.aplaboratories.com & Donna S. Ingersoll, B.S., HTL, CT(ASCP) Laboratory Manager A P Laboratories LLC 8 [5]dingersoll@aplaboratories.com References 1. 3D"mailto:Dingersoll@apl 2. 3D"mailto:histonet-request@lists.utsouthwes 3. file://localhost/tmp/3D"m 4. 3D"http://www.aplabor=/ 5. 3D"mailto:dingersoll@aplaboratories. From tony.henwood <@t> health.nsw.gov.au Tue Mar 5 18:05:19 2013 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Tue Mar 5 18:05:39 2013 Subject: [Histonet] Kappa/Lambda ISH In-Reply-To: <1362525680.80549.YahooMailNeo@web125404.mail.ne1.yahoo.com> References: <1362525680.80549.YahooMailNeo@web125404.mail.ne1.yahoo.com> Message-ID: <6D6BD1DE8A5571489398B392A38A71579D2357E0@xmdb04.nch.kids> Yep, Sure do. We use a multi-block of appendix (rich in plasma cells). Using the Bond 3, we put the test section on the same slide as the control. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Wilson A Sent: Wednesday, 6 March 2013 10:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Kappa/Lambda ISH ? ??? Hi, ??????? Please I am wondering whether you guys run controls with your Kappa/Lambda ISH. ? ???? Thanks, ???? Wilson _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From cls71877 <@t> gmail.com Tue Mar 5 19:33:18 2013 From: cls71877 <@t> gmail.com (Cristi Rigazio) Date: Tue Mar 5 19:33:28 2013 Subject: [Histonet] RE: Pregnancy In-Reply-To: <14E2C6176416974295479C64A11CB9AE016411AC279D@SBS2K8.premierlab.local> References: <930EB2E8DF68C544873EDD2A3D5F506007D0300D42@email1.skaggs.net> <14E2C6176416974295479C64A11CB9AE016411AC279D@SBS2K8.premierlab.local> Message-ID: <800B67BE-7ECC-46BE-AD14-C0299D02231C@gmail.com> I told my doctor everything I dealt with on a daily basis and he said it was no problem. I worked in a 400 sq ft space where we do everything including grossing! My son is perfect, but had I known about everything you all are saying I would have done things differently! Nothing is worth risking your little one. If I am lucky enough to have another I want to thank you all for the input as I will not do it again!! Sent from my iPhone On Mar 5, 2013, at 3:20 PM, Elizabeth Chlipala wrote: > Karen > > Are you sure it was workman's comp. Workman's comp applies to individuals injured on the job. > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > Laboratory Manager > Premier Laboratory, LLC > PO Box 18592 > Boulder, CO 80308 > Work (303) 682-3949 > Fax (303) 682-9060 > Cell (303) 881-0763 > liz@premierlab.com > www.premierlab.com > > Ship to address: > > 1567 Skyway Drive, Unit E > Longmont, CO 80504 > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Heckford, Karen - SMMC-SF > Sent: Tuesday, March 05, 2013 11:55 AM > To: 'Parker, Helayne'; histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: Pregnancy > > I was working in a Histology Lab when I first got pregnant. I told my doctor about everything I was exposed to in the lab. He actually opted on putting me on medical leave (workman's comp). I did this with both of my pregnancies. Not in less you can find her a job that she is not exposed. I would not risk exposure. > > Karen Heckford HT ASCP CE > Lead Histology Technician > St. Mary's Medical Center > 450 Stanyan St. > San Francisco, Ca. 94117 > 415-668-1000 ext. 6167 > karen.heckford@dignityhealth.org > Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Parker, Helayne > Sent: Tuesday, March 05, 2013 10:10 AM > To: 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] Pregnancy > > Hi Gang, > Thought I would ask some people with some more experience than I in this. One of our techs is pregnant- Yay !!! She is very early - about 5 weeks. My Lab Director wants her to start wearing a mask due to the chemicals. So we need to ask what sort of precautions need to take place during the pregnancy etc. We also coverslip by hand here out of xylene etc. I can not remember ever working with any pregnant HTs in the past so I honestly do not know. Please carbon copy replies to JAScholefield@skaggs.net as well. We predominately work with 10% formalin, reagent grade alcohols, paraplast, xylenes, clear-rite 3, Decal I and II (from Surgipath), cytofixative and some routine special stain items. Basically the typical myriad of stuff a very small routine histo lab would have (no immunos).... > > Thanks and bunch for your help ! > > > Sincerely, > > Helayne Parker, H.T. (ASCP) > Pathology Section Head > Cox Medical Center Branson > P.O. Box 650, Branson, MO 65615 > Phone: 417-335-7254 > Fax: 417-335-7127 > Email: hparker@skaggs.net > Web: www.coxhealth.com/branson > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From debgranato <@t> yahoo.com Tue Mar 5 21:11:08 2013 From: debgranato <@t> yahoo.com (Debbie Granato) Date: Tue Mar 5 21:11:17 2013 Subject: [Histonet] Grossing Qualifications Message-ID: <1362539468.97214.YahooMailClassic@web161206.mail.bf1.yahoo.com> We have an? employee who has attended a School of Medical Technology Program for one year (1974-75) and passed the registry in 1975. They have grossed since 2006 and have 8 hours of biology from a local college. Do these qualifications make them eligible to gross in prostate biopsies in an in- house laboratory? ?We are going over the requirements and are not sure if the experience that she has counts for her grossing in our lab. Does the time in the school fulfill the requirements? From b427297 <@t> aol.com Wed Mar 6 03:56:45 2013 From: b427297 <@t> aol.com (Jackie O'Connor) Date: Wed Mar 6 03:56:48 2013 Subject: [Histonet] Ethanol denatured with toluene In-Reply-To: <1362539468.97214.YahooMailClassic@web161206.mail.bf1.yahoo.com> References: <1362539468.97214.YahooMailClassic@web161206.mail.bf1.yahoo.com> Message-ID: <8CFE8680BBA6BDD-1434-C6005@webmail-m157.sysops.aol.com> I am currently in Germany running a QC inspection of a histology division. The histo lab uses ethanol denatured with toluene - I have never come across this before. I have been trying to troubleshoot their H+E stain, and I've been stumped for a long time as to why the sharpness and crispness I'm looking for isn't quite right. Could the toluene in the ethanol used to make dilutions be the culprit? I've not been able to find any references to toluene use in US products. Any input would be greatly appreciated. Jackie O' From b427297 <@t> aol.com Wed Mar 6 04:50:59 2013 From: b427297 <@t> aol.com (Jackie O'Connor) Date: Wed Mar 6 04:51:47 2013 Subject: [Histonet] RE: Pregnancy In-Reply-To: <800B67BE-7ECC-46BE-AD14-C0299D02231C@gmail.com> References: <930EB2E8DF68C544873EDD2A3D5F506007D0300D42@email1.skaggs.net> <14E2C6176416974295479C64A11CB9AE016411AC279D@SBS2K8.premierlab.local> <800B67BE-7ECC-46BE-AD14-C0299D02231C@gmail.com> Message-ID: <8CFE86F9F57FF79-136C-18C@webmail-d167.sysops.aol.com> We currently have a lovely pregnant lady in our histo lab, and although our EHS did not see the need, we are opting to keep her away from xylene and formalin, so no wet trimming of tissues (we do animal research), no changing processors, and no staining/coverslipping. There are many other tasks she can do, and her wonderful coworkers are happy to pick up the tasks where these nasty chemicals are present. I always recommend you speak to your OB about potential hazards - the OB I consult with believes that if the chemicals (per PPE) do not pose a hazard to the Mom, the baby will be fine, since the placenta is a great (not perfect) filter. He is a high risk OB, and I trust him implicitly, but your own doc knows best. Some docs will put you on full restrictions (which in some workplaces means medical leave for your entire pregnancy) He did recommend my daughter stay away from cyno monkeys during her pregnancy, which she did. I WISH NSH would do a study on pregancy in histology labs, as well as run a retrospective study on miscarriage and birth defect rates in histo techs. I'm sure every histotech would benefit from such information. Jackie O' -----Original Message----- From: Cristi Rigazio To: Elizabeth Chlipala Cc: histonet ; Parker, Helayne ; Heckford, Karen - SMMC-SF Sent: Tue, Mar 5, 2013 7:34 pm Subject: Re: [Histonet] RE: Pregnancy I told my doctor everything I dealt with on a daily basis and he said it was no roblem. I worked in a 400 sq ft space where we do everything including rossing! My son is perfect, but had I known about everything you all are aying I would have done things differently! Nothing is worth risking your ittle one. If I am lucky enough to have another I want to thank you all for he input as I will not do it again!! Sent from my iPhone On Mar 5, 2013, at 3:20 PM, Elizabeth Chlipala wrote: > Karen Are you sure it was workman's comp. Workman's comp applies to individuals njured on the job. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Laboratory Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 Work (303) 682-3949 Fax (303) 682-9060 Cell (303) 881-0763 liz@premierlab.com www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] n Behalf Of Heckford, Karen - SMMC-SF Sent: Tuesday, March 05, 2013 11:55 AM To: 'Parker, Helayne'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Pregnancy I was working in a Histology Lab when I first got pregnant. I told my doctor bout everything I was exposed to in the lab. He actually opted on putting me n medical leave (workman's comp). I did this with both of my pregnancies. Not n less you can find her a job that she is not exposed. I would not risk xposure. Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford@dignityhealth.org aution: This email message, including all content and attachments, is ONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information ontained in this email message is intended only for the use of the recipient(s) amed above. If the reader of this message is not the intended recipient or an gent responsible for delivering it to the intended recipient, you have received his document in error. Any further review, dissemination, distribution, or opying of this message is strictly prohibited. If you have received this ommunication in error, please notify us immediately by reply email. Thank ou." -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] n Behalf Of Parker, Helayne Sent: Tuesday, March 05, 2013 10:10 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Pregnancy Hi Gang, Thought I would ask some people with some more experience than I in this. ne of our techs is pregnant- Yay !!! She is very early - about 5 weeks. My ab Director wants her to start wearing a mask due to the chemicals. So we need o ask what sort of precautions need to take place during the pregnancy etc. We lso coverslip by hand here out of xylene etc. I can not remember ever working ith any pregnant HTs in the past so I honestly do not know. Please carbon copy eplies to JAScholefield@skaggs.net as well. We predominately work with 10% ormalin, reagent grade alcohols, paraplast, xylenes, clear-rite 3, Decal I and I (from Surgipath), cytofixative and some routine special stain items. asically the typical myriad of stuff a very small routine histo lab would have no immunos).... Thanks and bunch for your help ! Sincerely, Helayne Parker, H.T. (ASCP) Pathology Section Head Cox Medical Center Branson P.O. Box 650, Branson, MO 65615 Phone: 417-335-7254 Fax: 417-335-7127 Email: hparker@skaggs.net Web: www.coxhealth.com/branson _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ istonet mailing list istonet@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet From chak_bou <@t> yahoo.com Wed Mar 6 06:30:05 2013 From: chak_bou <@t> yahoo.com (Chakib Boussahmain) Date: Wed Mar 6 06:30:10 2013 Subject: [Histonet] Sox-10 Message-ID: <1362573005.22660.YahooMailClassic@web160805.mail.bf1.yahoo.com> Hi Histonetters, I am just wondering if anyone is using SOX-10 antibody from Sigma? Any input will be appreciated! Thank you Chakib Histotechnologist HTL(ASCP) From ibernard <@t> uab.edu Wed Mar 6 06:35:01 2013 From: ibernard <@t> uab.edu (Ian R Bernard) Date: Wed Mar 6 06:35:27 2013 Subject: [Histonet] Grossing Qualifications In-Reply-To: <1362539468.97214.YahooMailClassic@web161206.mail.bf1.yahoo.com> References: <1362539468.97214.YahooMailClassic@web161206.mail.bf1.yahoo.com> Message-ID: Just did some work on this. There is a difference in non-pathologist processing versus grossing of specimens. The prostate biopsies is considered a processing versus a grossing. CAP and thus CLIA 88 states these as the qualifications for grossing For a technician to perform grossing procedures, he or she must possess either an associate degree or educational equivalent i.e. completion of 60 semester hours from an accredited institution which include 24 hours of medical laboratory technology courses or 24 hours of science courses that includes 6 semester hours of biology, chemistry or medical laboratory technology in any combination, or earn an associate degree in a laboratory science or medical laboratory technology, obtained from an accredited institution -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Debbie Granato Sent: Tuesday, March 05, 2013 9:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Grossing Qualifications We have an? employee who has attended a School of Medical Technology Program for one year (1974-75) and passed the registry in 1975. They have grossed since 2006 and have 8 hours of biology from a local college. Do these qualifications make them eligible to gross in prostate biopsies in an in- house laboratory? ?We are going over the requirements and are not sure if the experience that she has counts for her grossing in our lab. Does the time in the school fulfill the requirements? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BSullivan <@t> virtua.org Wed Mar 6 06:38:40 2013 From: BSullivan <@t> virtua.org (Sullivan, Beatrice) Date: Wed Mar 6 06:38:45 2013 Subject: [Histonet] Grossing Qualifications In-Reply-To: References: <1362539468.97214.YahooMailClassic@web161206.mail.bf1.yahoo.com> Message-ID: <6932520047F7EE46B512E9801344F160164EDC42@EXCHANGEMB-2.Virtua.org> The standard was changed for CAP. There is no more "processing" vs. "grossing". -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ian R Bernard Sent: Wednesday, March 06, 2013 7:35 AM To: Debbie Granato; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Grossing Qualifications Just did some work on this. There is a difference in non-pathologist processing versus grossing of specimens. The prostate biopsies is considered a processing versus a grossing. CAP and thus CLIA 88 states these as the qualifications for grossing For a technician to perform grossing procedures, he or she must possess either an associate degree or educational equivalent i.e. completion of 60 semester hours from an accredited institution which include 24 hours of medical laboratory technology courses or 24 hours of science courses that includes 6 semester hours of biology, chemistry or medical laboratory technology in any combination, or earn an associate degree in a laboratory science or medical laboratory technology, obtained from an accredited institution -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Debbie Granato Sent: Tuesday, March 05, 2013 9:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Grossing Qualifications We have an? employee who has attended a School of Medical Technology Program for one year (1974-75) and passed the registry in 1975. They have grossed since 2006 and have 8 hours of biology from a local college. Do these qualifications make them eligible to gross in prostate biopsies in an in- house laboratory? ?We are going over the requirements and are not sure if the experience that she has counts for her grossing in our lab. Does the time in the school fulfill the requirements? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message, and any included attachments, are from Virtua Health or its related affiliates and is intended only for the addressee(s). The information contained herein is privileged, proprietary or may include confidential information and/or protected patient health information. Any unauthorized review, forwarding, printing, copying, distributing, or otherwise disseminating or taking any action based on such information is strictly prohibited. If you have received this message in error, or have reason to believe you are not authorized to receive it, please delete this message promptly and notify the sender by e-mail with a copy to ISSECURITY@virtua.org. Thank you From pinkladyjmb <@t> yahoo.com Wed Mar 6 07:48:42 2013 From: pinkladyjmb <@t> yahoo.com (Jennifer Blanchard) Date: Wed Mar 6 07:48:45 2013 Subject: [Histonet] Jennifer Blanchard Message-ID: <1362577722.96085.YahooMailNeo@web142502.mail.bf1.yahoo.com> lvpiyyqmxtzxvuz rpx http://www.resoleviaggi.com/bn/jezqbazgqqslq/gtuvrbcppzqs/guwjliwjzjcxpl scbdm ozxoyzrqegx xtneaptgtnolaab qqpwfdlqjg From Terri.Brown <@t> Northside.com Wed Mar 6 07:51:35 2013 From: Terri.Brown <@t> Northside.com (Terri Brown) Date: Wed Mar 6 07:51:52 2013 Subject: [Histonet] Grossing Qualifications In-Reply-To: <6932520047F7EE46B512E9801344F160164EDC42@EXCHANGEMB-2.Virtua.org> References: <1362539468.97214.YahooMailClassic@web161206.mail.bf1.yahoo.com> <6932520047F7EE46B512E9801344F160164EDC42@EXCHANGEMB-2.Virtua.org> Message-ID: <731941C266951A47BEF11E5EFAAED9C9196548DB@nsmvexch01.northside.local> What kind of competency do you do for the Histology Tech that this type of processing? Terri H. Brown,, HT (ASCP) Pathology Laboratory Manager Northside Hospital Atlanta terri.brown@northside.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sullivan, Beatrice Sent: Wednesday, March 06, 2013 7:39 AM To: Ian R Bernard; Debbie Granato; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Grossing Qualifications The standard was changed for CAP. There is no more "processing" vs. "grossing". -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ian R Bernard Sent: Wednesday, March 06, 2013 7:35 AM To: Debbie Granato; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Grossing Qualifications Just did some work on this. There is a difference in non-pathologist processing versus grossing of specimens. The prostate biopsies is considered a processing versus a grossing. CAP and thus CLIA 88 states these as the qualifications for grossing For a technician to perform grossing procedures, he or she must possess either an associate degree or educational equivalent i.e. completion of 60 semester hours from an accredited institution which include 24 hours of medical laboratory technology courses or 24 hours of science courses that includes 6 semester hours of biology, chemistry or medical laboratory technology in any combination, or earn an associate degree in a laboratory science or medical laboratory technology, obtained from an accredited institution -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Debbie Granato Sent: Tuesday, March 05, 2013 9:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Grossing Qualifications We have an? employee who has attended a School of Medical Technology Program for one year (1974-75) and passed the registry in 1975. They have grossed since 2006 and have 8 hours of biology from a local college. Do these qualifications make them eligible to gross in prostate biopsies in an in- house laboratory? ?We are going over the requirements and are not sure if the experience that she has counts for her grossing in our lab. Does the time in the school fulfill the requirements? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message, and any included attachments, are from Virtua Health or its related affiliates and is intended only for the addressee(s). The information contained herein is privileged, proprietary or may include confidential information and/or protected patient health information. Any unauthorized review, forwarding, printing, copying, distributing, or otherwise disseminating or taking any action based on such information is strictly prohibited. If you have received this message in error, or have reason to believe you are not authorized to receive it, please delete this message promptly and notify the sender by e-mail with a copy to ISSECURITY@virtua.org. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by Northside Hospital. It may contain information that is confidential, privileged, proprietary, or otherwise legally exempt from disclosure. If you are not the intended recipient, you are hereby notified that you are not authorized to read, print, retain, copy or disseminate this message, any part of it, or any attachments. If you have received this message in error, please delete this message and any attachments from your system without reading the content and notify the sender immediately of the inadvertent transmission. There is no intent on the part of the sender to waive any privilege. From Caroline.Pratt <@t> uphs.upenn.edu Wed Mar 6 08:15:04 2013 From: Caroline.Pratt <@t> uphs.upenn.edu (Pratt, Caroline) Date: Wed Mar 6 08:15:10 2013 Subject: [Histonet] Grossing Qualifications In-Reply-To: <6932520047F7EE46B512E9801344F160164EDC42@EXCHANGEMB-2.Virtua.org> References: <1362539468.97214.YahooMailClassic@web161206.mail.bf1.yahoo.com> <6932520047F7EE46B512E9801344F160164EDC42@EXCHANGEMB-2.Virtua.org> Message-ID: Your tech is grandfathered in because she was grossing prior to 88. You just need documentation to prove it. We have a similar situation in our lab. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sullivan, Beatrice Sent: Wednesday, March 06, 2013 7:39 AM To: Ian R Bernard; Debbie Granato; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Grossing Qualifications The standard was changed for CAP. There is no more "processing" vs. "grossing". -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ian R Bernard Sent: Wednesday, March 06, 2013 7:35 AM To: Debbie Granato; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Grossing Qualifications Just did some work on this. There is a difference in non-pathologist processing versus grossing of specimens. The prostate biopsies is considered a processing versus a grossing. CAP and thus CLIA 88 states these as the qualifications for grossing For a technician to perform grossing procedures, he or she must possess either an associate degree or educational equivalent i.e. completion of 60 semester hours from an accredited institution which include 24 hours of medical laboratory technology courses or 24 hours of science courses that includes 6 semester hours of biology, chemistry or medical laboratory technology in any combination, or earn an associate degree in a laboratory science or medical laboratory technology, obtained from an accredited institution -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Debbie Granato Sent: Tuesday, March 05, 2013 9:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Grossing Qualifications We have an? employee who has attended a School of Medical Technology Program for one year (1974-75) and passed the registry in 1975. They have grossed since 2006 and have 8 hours of biology from a local college. Do these qualifications make them eligible to gross in prostate biopsies in an in- house laboratory? ?We are going over the requirements and are not sure if the experience that she has counts for her grossing in our lab. Does the time in the school fulfill the requirements? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message, and any included attachments, are from Virtua Health or its related affiliates and is intended only for the addressee(s). The information contained herein is privileged, proprietary or may include confidential information and/or protected patient health information. Any unauthorized review, forwarding, printing, copying, distributing, or otherwise disseminating or taking any action based on such information is strictly prohibited. If you have received this message in error, or have reason to believe you are not authorized to receive it, please delete this message promptly and notify the sender by e-mail with a copy to ISSECURITY@virtua.org. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From rsrichmond <@t> gmail.com Wed Mar 6 09:24:52 2013 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Wed Mar 6 09:24:57 2013 Subject: [Histonet] Re: Ethanol denatured with toluene Message-ID: Jackie O'Connor writes: >>I am currently in Germany running a QC inspection of a histology division. The histo lab uses ethanol denatured with toluene - I have never come across this before. I have been trying to troubleshoot their H+E stain, and I've been stumped for a long time as to why the sharpness and crispness I'm looking for isn't quite right. Could the toluene in the ethanol used to make dilutions be the culprit? I've not been able to find any references to toluene use in US products. Any input would be greatly appreciated.<< I've never encountered denaturation with toluene before, and I'm not sure it's a BATF-approved denaturant in the US. The German Wikipedia http://de.wikipedia.org/wiki/Verg%C3%A4llung makes reference to denaturation with toluene ("Toluol" in German). I think toluene is a poor choice for a denaturant, because of its toxicity (similar to xylene and benzene), and because it may dissolve some dyes out of tissue sections. The German Wikipedia article doesn't mention anything like our reagent alcohol (90% ethanol, 5% methanol, 5% isopropanol). I'd find out what other histology labs in Germany use. Wikipedia lists a number of possibilities - I'm going to leave this in German, since your client will need to look at it anyway: >>F?r die Verwendung als Rohstoff in der chemischen Industrie sind g?ngige Verg?llungsmittel Methylethylketon, Petrolether, Toluol und Cyclohexan. Weitere zugelassene Stoffe sind Schellack, Fichtenkolophonium, Phthals?urediethylester, Thymol, Diethylether und tert-Butanol in Verbindung mit Isopropanol oder Denatoniumbenzoat.<< Bob Richmond Samurai Pathologist Maryville, Tennessee USA From ree3 <@t> leicester.ac.uk Wed Mar 6 09:30:45 2013 From: ree3 <@t> leicester.ac.uk (Edwards, Richard E.) Date: Wed Mar 6 09:31:05 2013 Subject: [Histonet] Re: Ethanol denatured with toluene In-Reply-To: References: Message-ID: <7722595275A4DD4FA225B92CDBF174A101B0C795E335@EXC-MBX3.cfs.le.ac.uk> We used to use toluene on our tissue processor, it replaced benzene, ah the good old days!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: 06 March 2013 15:25 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Ethanol denatured with toluene Jackie O'Connor writes: >>I am currently in Germany running a QC inspection of a histology division. The histo lab uses ethanol denatured with toluene - I have never come across this before. I have been trying to troubleshoot their H+E stain, and I've been stumped for a long time as to why the sharpness and crispness I'm looking for isn't quite right. Could the toluene in the ethanol used to make dilutions be the culprit? I've not been able to find any references to toluene use in US products. Any input would be greatly appreciated.<< I've never encountered denaturation with toluene before, and I'm not sure it's a BATF-approved denaturant in the US. The German Wikipedia http://de.wikipedia.org/wiki/Verg%C3%A4llung makes reference to denaturation with toluene ("Toluol" in German). I think toluene is a poor choice for a denaturant, because of its toxicity (similar to xylene and benzene), and because it may dissolve some dyes out of tissue sections. The German Wikipedia article doesn't mention anything like our reagent alcohol (90% ethanol, 5% methanol, 5% isopropanol). I'd find out what other histology labs in Germany use. Wikipedia lists a number of possibilities - I'm going to leave this in German, since your client will need to look at it anyway: >>F?r die Verwendung als Rohstoff in der chemischen Industrie sind >>g?ngige Verg?llungsmittel Methylethylketon, Petrolether, Toluol und >>Cyclohexan. Weitere zugelassene Stoffe sind Schellack, >>Fichtenkolophonium, Phthals?urediethylester, Thymol, Diethylether und >>tert-Butanol in Verbindung mit Isopropanol oder Denatoniumbenzoat.<< Bob Richmond Samurai Pathologist Maryville, Tennessee USA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> gmail.com Wed Mar 6 09:33:17 2013 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Wed Mar 6 09:33:23 2013 Subject: [Histonet] Re: Pregnancy Message-ID: Helayne Parker is concerned about a woman 5 weeks pregnant working in an ordinary histopathology lab. I think the major issue is xylene (along with toluene and benzene). If the lab uses xylene in tissue processing and staining, I don't think she should be around it. If the lab is otherwise xylene-free, the mounting medium probably contains an aromatic hydrocarbon, and I don't think she should be coverslipping even under a proper hood, since xylene is readily absorbed through the skin. I think formaldehyde depends on ventilation. If the lab's as badly ventilated as most labs I work in, then I wouldn't want her to be around it. She should definitely run the problem past her OB-GYN, but I wouldn't want to put her doctor in the position of having to decide for her, simply because the problem is so far out of the doctor's field of expertise. Remember that very few pathologists (let alone clinicians) know as much about the materials science of histology as I do! Bob Richmond Samurai Pathologist Maryville, TN From cheastys <@t> svm.vetmed.wisc.edu Wed Mar 6 09:35:06 2013 From: cheastys <@t> svm.vetmed.wisc.edu (Sandra Cheasty) Date: Wed Mar 6 09:35:07 2013 Subject: [Histonet] DAB source needed Message-ID: Hello All, Can anyone recommend a DAB kit that is reliable and reasonable priced? Also, can someone shovel my driveway? Thanks! Sandy Sandra Cheasty Histology & Necropsy Supervisor UW-Madison, Wisconsin From dlne <@t> att.net Wed Mar 6 09:49:07 2013 From: dlne <@t> att.net (Dermpath Lab) Date: Wed Mar 6 09:49:10 2013 Subject: [Histonet] (no subject) Message-ID: <1362584947.28602.YahooMailClassic@web181605.mail.ne1.yahoo.com> Hi! I was just wondering what different facilities use for red ink/dye.? Some of the new ones we have tried gets too light or washes off during processing.? Thanks! From lblazek <@t> digestivespecialists.com Wed Mar 6 09:48:51 2013 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Wed Mar 6 09:50:17 2013 Subject: [Histonet] RE: DAB source needed In-Reply-To: References: Message-ID: <5A2BD13465E061429D6455C8D6B40E39164D40DC0E@IBMB7Exchange.digestivespecialists.com> I have retired from shoveling but try Bio Care for your DAB. They have a very nice one. Also if you find someone to shovel send them my way! Although Wisconsin to Ohio might be tricky. But thanks for sending the snow our way. :) Linda Linda Blazek HT (ASCP) Manager/Supervisor Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sandra Cheasty Sent: Wednesday, March 06, 2013 10:35 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] DAB source needed Hello All, Can anyone recommend a DAB kit that is reliable and reasonable priced? Also, can someone shovel my driveway? Thanks! Sandy Sandra Cheasty Histology & Necropsy Supervisor UW-Madison, Wisconsin _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cmiller <@t> gladstone.ucsf.edu Wed Mar 6 09:50:21 2013 From: cmiller <@t> gladstone.ucsf.edu (Caroline Miller) Date: Wed Mar 6 09:50:27 2013 Subject: [Histonet] Re: Pregnancy In-Reply-To: References: Message-ID: <132EC587-F095-44A6-BDCA-B317F8A471B4@gladstone.ucsf.edu> I was recently pregnant (I have a happy and healthy 8month old). I work in a research lab and did come in contact with xylene at 12 weeks when our processor went crazy and we had a whole batch of processing go wrong on us. I was pretty scared that the xylene had affected the baby so I got a xylene exposure badge from the h+s dept and we did a test. Even with the stainer open and the mounting hood at its top limit we didn't even come close to the legal limit of xylene exposure. I suppose my point is that new fume hoods do their job really well. I would certainly stay away from changing the Machine Caroline Miller Gladstone Institutes www.gladstoneinstitutes.org Tel: 415 7342566 Cell: 415 2187297 On Mar 6, 2013, at 7:33 AM, Bob Richmond wrote: > Helayne Parker is concerned about a woman 5 weeks pregnant working in > an ordinary histopathology lab. > > I think the major issue is xylene (along with toluene and benzene). If > the lab uses xylene in tissue processing and staining, I don't think > she should be around it. If the lab is otherwise xylene-free, the > mounting medium probably contains an aromatic hydrocarbon, and I don't > think she should be coverslipping even under a proper hood, since > xylene is readily absorbed through the skin. > > I think formaldehyde depends on ventilation. If the lab's as badly > ventilated as most labs I work in, then I wouldn't want her to be > around it. > > She should definitely run the problem past her OB-GYN, but I wouldn't > want to put her doctor in the position of having to decide for her, > simply because the problem is so far out of the doctor's field of > expertise. Remember that very few pathologists (let alone clinicians) > know as much about the materials science of histology as I do! > > Bob Richmond > Samurai Pathologist > Maryville, TN > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dlne <@t> att.net Wed Mar 6 09:50:59 2013 From: dlne <@t> att.net (Dermpath Lab) Date: Wed Mar 6 09:51:02 2013 Subject: Fw: [Histonet] (no subject) Message-ID: <1362585059.3320.YahooMailClassic@web181601.mail.ne1.yahoo.com> --- On Wed, 3/6/13, Dermpath Lab wrote: From: Dermpath Lab Subject: [Histonet] (no subject) To: histonet@lists.utsouthwestern.edu Date: Wednesday, March 6, 2013, 10:49 AM Hi! I was just wondering what different facilities use for red ink/dye.? Some of the new ones we have tried gets too light or washes off during processing.? Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cmiller <@t> gladstone.ucsf.edu Wed Mar 6 09:52:51 2013 From: cmiller <@t> gladstone.ucsf.edu (Caroline Miller) Date: Wed Mar 6 09:53:02 2013 Subject: [Histonet] Re: Pregnancy In-Reply-To: <132EC587-F095-44A6-BDCA-B317F8A471B4@gladstone.ucsf.edu> References: <132EC587-F095-44A6-BDCA-B317F8A471B4@gladstone.ucsf.edu> Message-ID: (Sorry hit send too soon, see below) Caroline Miller Gladstone Institutes www.gladstoneinstitutes.org Tel: 415 7342566 Cell: 415 2187297 On Mar 6, 2013, at 7:50 AM, Caroline Miller wrote: > I was recently pregnant (I have a happy and healthy 8month old). I work in a research lab and did come in contact with xylene at 12 weeks when our processor went crazy and we had a whole batch of processing go wrong on us. > > I was pretty scared that the xylene had affected the baby so I got a xylene exposure badge from the h+s dept and we did a test. Even with the stainer open and the mounting hood at its top > limit we didn't even come close to the legal limit of xylene exposure. > > I suppose my point is that new fume hoods do their job really well. I would certainly stay away from changing the > Machine and limit exposure, but I don't think there is any real danger with care and attention by the pregnant lady and her colleagues > > Caroline Miller > Gladstone Institutes > www.gladstoneinstitutes.org > > Tel: 415 7342566 > Cell: 415 2187297 > > > > > On Mar 6, 2013, at 7:33 AM, Bob Richmond wrote: > >> Helayne Parker is concerned about a woman 5 weeks pregnant working in >> an ordinary histopathology lab. >> >> I think the major issue is xylene (along with toluene and benzene). If >> the lab uses xylene in tissue processing and staining, I don't think >> she should be around it. If the lab is otherwise xylene-free, the >> mounting medium probably contains an aromatic hydrocarbon, and I don't >> think she should be coverslipping even under a proper hood, since >> xylene is readily absorbed through the skin. >> >> I think formaldehyde depends on ventilation. If the lab's as badly >> ventilated as most labs I work in, then I wouldn't want her to be >> around it. >> >> She should definitely run the problem past her OB-GYN, but I wouldn't >> want to put her doctor in the position of having to decide for her, >> simply because the problem is so far out of the doctor's field of >> expertise. Remember that very few pathologists (let alone clinicians) >> know as much about the materials science of histology as I do! >> >> Bob Richmond >> Samurai Pathologist >> Maryville, TN >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet From campbellj <@t> muhlbauerlab.com Wed Mar 6 10:18:38 2013 From: campbellj <@t> muhlbauerlab.com (Jennifer Campbell) Date: Wed Mar 6 10:18:44 2013 Subject: [Histonet] (no subject) In-Reply-To: <1362584947.28602.YahooMailClassic@web181605.mail.ne1.yahoo.com> References: <1362584947.28602.YahooMailClassic@web181605.mail.ne1.yahoo.com> Message-ID: We use India ink and then use 3% acetic acid to affix it to the specimen. Works great on ellipse excisions as well as small slices. Jen Campbell On Wed, Mar 6, 2013 at 10:49 AM, Dermpath Lab wrote: > Hi! I was just wondering what different facilities use for red ink/dye. > Some of the new ones we have tried gets too light or washes off during > processing. Thanks! > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Jen Campbell, BS, HT(ASCP) Supervisor of Technical Services Muhlbauer Dermatopathology Laboratory 61 Monroe Avenue, Ste B Pittsford NY 14534 P: 585.586.5166 F: 585.586.3137 IMPORTANT NOTICE: This e-mail and any attachments may contain confidential or sensitive information which is, or may be, legally privileged or otherwise protected by law from further disclosure. It is intended only for the addressee. If you received this in error or from someone who was not authorized to send it to you, please do not distribute, copy or use it or any attachments. Please notify the sender immediately by reply e-mail and delete this from your system. Thank you for your cooperation. From rjbuesa <@t> yahoo.com Wed Mar 6 10:52:38 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Mar 6 10:52:43 2013 Subject: [Histonet] Ethanol denatured with toluene In-Reply-To: <8CFE8680BBA6BDD-1434-C6005@webmail-m157.sysops.aol.com> References: <1362539468.97214.YahooMailClassic@web161206.mail.bf1.yahoo.com> <8CFE8680BBA6BDD-1434-C6005@webmail-m157.sysops.aol.com> Message-ID: <1362588758.2521.YahooMailNeo@web163106.mail.bf1.yahoo.com> Probably. Toluene is a clearant. Perhaps they are doing that following a very old recipe or some pathologist's preferences, but I do not understand the chemical rational behind that mixture. ren? J. From: Jackie O'Connor To: histonet@lists.utsouthwestern.edu Sent: Wednesday, March 6, 2013 4:56 AM Subject: [Histonet] Ethanol denatured with toluene I am currently in Germany running a QC inspection of a histology division.? The histo lab uses ethanol denatured with toluene - I have never come across this before.? I have been trying to troubleshoot their H+E stain, and I've been stumped for a long time as to why the sharpness and crispness I'm looking for isn't quite right.? Could the toluene in the ethanol used to make dilutions be the culprit?? I've not been able to find any references to toluene use in US products.? Any input would be greatly appreciated. Jackie O' _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TJohnson <@t> gnf.org Wed Mar 6 11:11:36 2013 From: TJohnson <@t> gnf.org (Teri Johnson) Date: Wed Mar 6 11:11:40 2013 Subject: [Histonet] Re: Ethanol denatured with toluene Message-ID: <9F3CFEE76E51B64991C7485270890B404972C930@EX5.lj.gnf.org> Hi Jackie, The ethanol we use is denatured with a lot of stuff that can include methanol, MIBK, Toluene, and ethyl acetate , and our nuclear stains are crisp and sharp. I would start looking at fixation first, followed by their H&E stain procedure and reagents. Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 From LangleKL <@t> stlukes.org Wed Mar 6 11:59:08 2013 From: LangleKL <@t> stlukes.org (Langley, Kristi L.) Date: Wed Mar 6 11:59:18 2013 Subject: [Histonet] Re: Inking esophagus biopsies In-Reply-To: References: Message-ID: <244DF79909BD6D4EB7685572698EF1F501A0FE9C@TDCEXCHXM004.ihs.org> Do people still use hematoxilyn? I went from one lab using safranin to now using hematox....I can not get them to switch either...in my opinion safranin works so much better, but they are not open to any changes of any kind......:( and if I offer any I am even told I am questioning authority..Help me find documentation to prove my case Kristi Langley HT(ASCP)cm St. Luke's Medical Center 2720 Stone Park Blvd. Sioux City, Ia 51104 712-279-3164 ext 6 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Bob Richmond [rsrichmond@gmail.com] Sent: Wednesday, December 12, 2012 12:17 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Inking esophagus biopsies Sheila Adey (somewhere in Canada) asks: Can anyone reccommend a good way to ink esophageal biopsies without using mercurochrome? Out of many pathology services I've worked on, I've only seen one that marked GI biopsies when grossing. They used safranin O (the routine Gram stain counterstain, from the microbiology lab) and it worked quite well. I would not use eosin because it makes fluorescence microscopy impossible. You can't get mercurochrome any more (in the US anyway), and you don't want the mercury in your processor anyway. Bob Richmond Samurai Pathologist Maryville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message and accompanying documents are covered by the Electronic Communications Privacy Act, 18 U.S.C. sections 2510-2521, and contain information intended for the specified individual(s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying, or the taking of any action based on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From lisab <@t> hollandhospital.org Wed Mar 6 11:21:28 2013 From: lisab <@t> hollandhospital.org (Lisa Brenner) Date: Wed Mar 6 12:12:13 2013 Subject: [Histonet] ANP.23018 Message-ID: <513734C8.215E.003C.1@hollandhospital.org> Hello, I was hoping someone could give me their interpretation of what they are looking for in this checklist question. ANP.23018 Control materials at more than one expression (level) are run concurrently with patient specimens. Note: Controls should verify test performance at relevant decision points. For many tests, a positive and negative control are sufficient. Controls need be run only on days when patient specimens are tested. For immunohistochemistry, the laboratory must follow the control requirements in the immunohistochemistry section of this checklist. This question is under quality control. Kind of under validation and calibration all under digital image analysis. I follow all the control requirements for immuno, special stains, and daily H&E QC. What are they looking for? Thanks in advance for your input. Lisa Lisa Brenner HTL Histology Lab Holland Hospital 602 Michigan Ave. Holland, MI 49423 (616)394-3184 Confidentiality Notice: The information contained in this e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information, or Protected Health Information as such term is defined under the Health Insurance Portability and Accountability Act of 1996 (HIPAA). Any unauthorized review, use, disclosure, copying or distribution is prohibited and may be unlawful. If you believe you have received this e-mail in error, please contact the sender by reply e-mail and delete all copies of the original message, including attachments. From Timothy.Morken <@t> ucsfmedctr.org Wed Mar 6 12:32:59 2013 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Wed Mar 6 12:33:17 2013 Subject: [Histonet] ANP.23018 In-Reply-To: <513734C8.215E.003C.1@hollandhospital.org> References: <513734C8.215E.003C.1@hollandhospital.org> Message-ID: <761E2B5697F795489C8710BCC72141FF061FF5@ex07.net.ucsf.edu> Lisa, I take this to mean that when you have an antigen for which expression level is a critical part of the interpretive decision that your control should have a full range of expression levels. The most important part of that is ensuring you are not missing low expression levels, for instance in ER staining. Or cut-off expression levels in Her2. Ideally you will have a protocol that delineates which antigens require that type of control and which of your controls meet the requirement. Of course you need a good validation of the control that shows the desired expression levels under suitable staining conditions. Tim Morken Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lisa Brenner Sent: Wednesday, March 06, 2013 9:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ANP.23018 Hello, I was hoping someone could give me their interpretation of what they are looking for in this checklist question. ANP.23018 Control materials at more than one expression (level) are run concurrently with patient specimens. Note: Controls should verify test performance at relevant decision points. For many tests, a positive and negative control are sufficient. Controls need be run only on days when patient specimens are tested. For immunohistochemistry, the laboratory must follow the control requirements in the immunohistochemistry section of this checklist. This question is under quality control. Kind of under validation and calibration all under digital image analysis. I follow all the control requirements for immuno, special stains, and daily H&E QC. What are they looking for? Thanks in advance for your input. Lisa Lisa Brenner HTL Histology Lab Holland Hospital 602 Michigan Ave. Holland, MI 49423 (616)394-3184 Confidentiality Notice: The information contained in this e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information, or Protected Health Information as such term is defined under the Health Insurance Portability and Accountability Act of 1996 (HIPAA). Any unauthorized review, use, disclosure, copying or distribution is prohibited and may be unlawful. If you believe you have received this e-mail in error, please contact the sender by reply e-mail and delete all copies of the original message, including attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Wed Mar 6 12:35:11 2013 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Wed Mar 6 12:35:21 2013 Subject: [Histonet] ANP.23018 In-Reply-To: <513734C8.215E.003C.1@hollandhospital.org> References: <513734C8.215E.003C.1@hollandhospital.org> Message-ID: <14E2C6176416974295479C64A11CB9AE016411AC27BE@SBS2K8.premierlab.local> Lisa First of all I want to state that I'm in a research lab and not a clinical lab. I believe that they are looking that the control tissues for IHC stains that are being used for image analysis (ER/PR, HER2, Ki-67, etc.) contain multiple tissue samples with different staining intensities, such as negative, 1+, 2+ and 3+ expression levels. What expression levels you use I believe CAP leaves up to you but I would think one tissue that is 1+ and one tissue that is 3+ would work. Others in the clinical setting may comment on this in more detail. Vendors will supply cell block controls with varying expression levels with these kits but ideally you should be using laboratory positive controls (tissue processed in your lab) and these should consist of multiple tissues so when stained they will demonstrate different expression levels. It's also important in the IHC protocol development and validation process that tissue of varying staining intensities be used also. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Laboratory Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 Work (303) 682-3949 Fax (303) 682-9060 Cell (303) 881-0763 liz@premierlab.com www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lisa Brenner Sent: Wednesday, March 06, 2013 10:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ANP.23018 Hello, I was hoping someone could give me their interpretation of what they are looking for in this checklist question. ANP.23018 Control materials at more than one expression (level) are run concurrently with patient specimens. Note: Controls should verify test performance at relevant decision points. For many tests, a positive and negative control are sufficient. Controls need be run only on days when patient specimens are tested. For immunohistochemistry, the laboratory must follow the control requirements in the immunohistochemistry section of this checklist. This question is under quality control. Kind of under validation and calibration all under digital image analysis. I follow all the control requirements for immuno, special stains, and daily H&E QC. What are they looking for? Thanks in advance for your input. Lisa Lisa Brenner HTL Histology Lab Holland Hospital 602 Michigan Ave. Holland, MI 49423 (616)394-3184 Confidentiality Notice: The information contained in this e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information, or Protected Health Information as such term is defined under the Health Insurance Portability and Accountability Act of 1996 (HIPAA). Any unauthorized review, use, disclosure, copying or distribution is prohibited and may be unlawful. If you believe you have received this e-mail in error, please contact the sender by reply e-mail and delete all copies of the original message, including attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Donna.Willis <@t> baylorhealth.edu Wed Mar 6 12:40:18 2013 From: Donna.Willis <@t> baylorhealth.edu (Willis, Donna G.) Date: Wed Mar 6 12:40:28 2013 Subject: [Histonet] ANP.23018 In-Reply-To: <761E2B5697F795489C8710BCC72141FF061FF5@ex07.net.ucsf.edu> References: <513734C8.215E.003C.1@hollandhospital.org> <761E2B5697F795489C8710BCC72141FF061FF5@ex07.net.ucsf.edu> Message-ID: <2572B4D63B62E64A8078D8BBE34D407804FA2B@BHDAMAEXML2.bhcs.pvt> If the question is listed under the Digital Image Analysis portion of the checklist and you do not perform IHC by a digital system then it is N/A. Donna Willis, HT/HTL (ASCP) Anatomic Pathology Manager Baylor University Medical Center-Dallas ph. 214-820-2465 office ph. 214-725-6184 mobile donna.willis@baylorhealth.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Wednesday, March 06, 2013 12:33 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP.23018 Lisa, I take this to mean that when you have an antigen for which expression level is a critical part of the interpretive decision that your control should have a full range of expression levels. The most important part of that is ensuring you are not missing low expression levels, for instance in ER staining. Or cut-off expression levels in Her2. Ideally you will have a protocol that delineates which antigens require that type of control and which of your controls meet the requirement. Of course you need a good validation of the control that shows the desired expression levels under suitable staining conditions. Tim Morken Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lisa Brenner Sent: Wednesday, March 06, 2013 9:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ANP.23018 Hello, I was hoping someone could give me their interpretation of what they are looking for in this checklist question. ANP.23018 Control materials at more than one expression (level) are run concurrently with patient specimens. Note: Controls should verify test performance at relevant decision points. For many tests, a positive and negative control are sufficient. Controls need be run only on days when patient specimens are tested. For immunohistochemistry, the laboratory must follow the control requirements in the immunohistochemistry section of this checklist. This question is under quality control. Kind of under validation and calibration all under digital image analysis. I follow all the control requirements for immuno, special stains, and daily H&E QC. What are they looking for? Thanks in advance for your input. Lisa Lisa Brenner HTL Histology Lab Holland Hospital 602 Michigan Ave. Holland, MI 49423 (616)394-3184 Confidentiality Notice: The information contained in this e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information, or Protected Health Information as such term is defined under the Health Insurance Portability and Accountability Act of 1996 (HIPAA). Any unauthorized review, use, disclosure, copying or distribution is prohibited and may be unlawful. If you believe you have received this e-mail in error, please contact the sender by reply e-mail and delete all copies of the original message, including attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This e-mail may contain confidential and/or privileged information. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. From lcates <@t> synecor.com Wed Mar 6 13:44:16 2013 From: lcates <@t> synecor.com (Lynne Cates) Date: Wed Mar 6 13:44:31 2013 Subject: [Histonet] Norepinephrine Message-ID: <8F48B67721A242408CC669457EC12ACFEA6A2D2D@EXCHANGE.CORP.synecor.local> I am looking for a lab that has the capability of doing norepinephrine testing on kidney shavings. It must be a validated GLP procedure. This is animal tissue. I would appreciate any help that anyone can provide. Lynne Cates, HT (ASCP) Synecor, LLC 3908 Patriot Drive Suite# 170 Durham, NC 27703 Phone 919-541-9977 x 119 lcates@synecor.com From pbourassa <@t> karospharma.com Thu Mar 7 07:36:18 2013 From: pbourassa <@t> karospharma.com (Patricia Bourassa) Date: Thu Mar 7 07:36:24 2013 Subject: [Histonet] Smooth muscle cell actin Message-ID: Howdy! Need to attempt immunostaining of smooth muscle cell actin in rat lung. Any recommendations for reliable SMC primary? Thanks in advance! Patti -- *Patti Bourassa Senior Scientist Karos Pharmaceuticals (203) 535-0540, ext 207* From AGleiberman <@t> cbiolabs.com Thu Mar 7 08:55:22 2013 From: AGleiberman <@t> cbiolabs.com (Anatoli Gleiberman) Date: Thu Mar 7 08:55:32 2013 Subject: [Histonet] Smooth muscle cell actin In-Reply-To: References: Message-ID: <77BC2EEB6AC66C49AEF794DC98BE314C013D04322A@cbiolabs05.CBiolabs.local> Patti, The best antibody (much better than any rabbit, goat or even the same 1A4 clone conjugated with FITC) are: Mouse monoclonal Anti-alpha-smooth muscle actin Cy3 conjugated, Sigma cat.No.C6198. Works in dilution 1:500-1:2000 on formaldehyde fixed cell cultures, cryosections (both from fresh frozen and formaldehyde fixed samples, paraffin after formalin etc). I work with it last 8 years without any problem. They are relatively expensive, but last for long due to high working dilution. Anatoli Gleiberman, PhD Director of Histopathology Cleveland Biolabs, Inc 73 High Street Buffalo, NY 14203 phone:716-849-6810 ext.354 fax:716-849-6817 e-mail: AGleiberman@cbiolabs.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patricia Bourassa Sent: Thursday, March 07, 2013 8:36 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Smooth muscle cell actin Howdy! Need to attempt immunostaining of smooth muscle cell actin in rat lung. Any recommendations for reliable SMC primary? Thanks in advance! Patti -- *Patti Bourassa Senior Scientist Karos Pharmaceuticals (203) 535-0540, ext 207* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information. From POWELL_SA <@t> mercer.edu Thu Mar 7 11:14:36 2013 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Thu Mar 7 11:14:41 2013 Subject: [Histonet] Question on Estrogen antibodies for fish Message-ID: <9BF995BC0E47744E9673A41486E24EE257974DA441@MERCERMAIL.MercerU.local> I am asking this for a researcher. Please respond to her at klar_elizabeth@columbusstate.edu. Her name is Ely Klar. She is looking for any form of estrogen antibody that works on bass fish gonads. Vendor's responses welcome. Shirley A. Powell, HT(ASCP)HTL, QIHC Technical Director Histology Curricular Support Laboratory Mercer University School of Medicine 1550 College Street Macon, GA 31207 478-301-2374 Lab 478-301-5489 Fax From soofias2 <@t> yahoo.com Thu Mar 7 11:21:41 2013 From: soofias2 <@t> yahoo.com (soofia siddiqui) Date: Thu Mar 7 11:21:47 2013 Subject: [Histonet] RE: DAB source needed In-Reply-To: <5A2BD13465E061429D6455C8D6B40E39164D40DC0E@IBMB7Exchange.digestivespecialists.com> References: <5A2BD13465E061429D6455C8D6B40E39164D40DC0E@IBMB7Exchange.digestivespecialists.com> Message-ID: <1362676901.9272.YahooMailNeo@web121905.mail.ne1.yahoo.com> I love Sigma DAB Tablets, they come in 1-5 and 15 MLs and you have to dissolve in water. The only problem is that they do not have any expression date. Soofia ________________________________ From: "Blazek, Linda" To: Sandra Cheasty ; "histonet@lists.utsouthwestern.edu" Sent: Wednesday, March 6, 2013 9:48 AM Subject: [Histonet] RE: DAB source needed I have retired from shoveling but try Bio Care for your DAB.? They have a very nice one.? Also if you find someone to shovel send them my way!? Although Wisconsin to Ohio might be tricky.? But thanks for sending the snow our way.? :) Linda Linda Blazek HT (ASCP) Manager/Supervisor Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sandra Cheasty Sent: Wednesday, March 06, 2013 10:35 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] DAB source needed Hello All, Can anyone recommend a DAB kit that is reliable and reasonable priced? Also, can someone shovel my driveway? Thanks! Sandy Sandra Cheasty Histology & Necropsy Supervisor UW-Madison, Wisconsin _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mhale <@t> MiracaLS.com Thu Mar 7 11:44:37 2013 From: mhale <@t> MiracaLS.com (Hale, Meredith) Date: Thu Mar 7 11:44:41 2013 Subject: [Histonet] KY HT Positions Message-ID: <0E828EC51C7CC445A51E53F81B64E8C71BEE31@s-irv-exchmb.PathologyPartners.intranet> Please no Recruiter calls Great opportunity for Histotechnician's in Crestview Hills, KY ! Tri-State Gastroenterology Associates is a multi-physician practice located in Northern Kentucky. Its mission is "To provide compassionate, high quality, cost-effective care to pateints with gastrointestinal problems" Looking for 2 Full Time HT/HTL's ( 32 hours a week is full time employement at this practice ) * Meet CLIA Grossing Requirements : CFR 493.1489, http://wwwn.cdc.gov/clia/regs/toc.aspx/ ,prior experience grossing GI specimens * Supervisor experience preferred * HT/HTL ASCP Certified * Experience with CLIA and CAP * Experience writing and maintaining policies and procedures * Prior laboratory start up experience is preferred * Ability to work independently Duties include: * Grossing * Embedding * Microtomy * Staining; routine and special stains only * Maintain supply orders and laboratory budget * Ability to be flexible and take on additional duties' as needed * Ability to work independently * Maintenance of laboratory for inspections * Maintenance of quality records Interested applicants should contact Meredith Hale; phone 214-596-2219 or through email mhale@miracals.com> Meredith Hale HT (ASCP)cm Director External Sales Support Miraca Life Sciences 6655 North MacArthur Blvd. Irving , Texas 75039 Office: 214-596-2219 Cell: 469-648-8253 Fax: 1-866-688-3280 mhale@miracals.com> From ncosenza <@t> siumed.edu Thu Mar 7 11:50:10 2013 From: ncosenza <@t> siumed.edu (Nicole Cosenza) Date: Thu Mar 7 11:50:14 2013 Subject: [Histonet] problem with DAB staining Message-ID: <5138D352.1070004@siumed.edu> Hello all: I recently did IHC on paraffin embedded slides via the DAB method. Upon addition of the DAB, the entire section turned brown. I know this is not true staining, as I don't expect a high yield of my protein of interest. Any suggestions as to why this happened and what to do about it? From shive003 <@t> umn.edu Thu Mar 7 11:56:37 2013 From: shive003 <@t> umn.edu (Jan Shivers) Date: Thu Mar 7 11:56:41 2013 Subject: [Histonet] problem with DAB staining In-Reply-To: <5138D352.1070004@siumed.edu> References: <5138D352.1070004@siumed.edu> Message-ID: Some suggestions: 1) your endogenous peroxidase was not quenched properly or entirely 2) your primary antibody is too concentrated 3) your secondary antibody is too concentrated 4) your incubation times are too long 5) your incubation temperatures are too high 6) your DAB was mixed up incorrectly 7) your rinses are insufficient in length 8) your rinses are insufficient in volume Jan Shivers UMN VDL St. Paul, MN On Thu, Mar 7, 2013 at 11:50 AM, Nicole Cosenza wrote: > Hello all: > > I recently did IHC on paraffin embedded slides via the DAB method. Upon > addition of the DAB, the entire section turned brown. I know this is not > true staining, as I don't expect a high yield of my protein of interest. > Any suggestions as to why this happened and what to do about it? > > ______________________________**_________________ > Histonet mailing list > Histonet@lists.utsouthwestern.**edu > http://lists.utsouthwestern.**edu/mailman/listinfo/histonet > From robert_schoonhoven <@t> yahoo.com Thu Mar 7 12:00:34 2013 From: robert_schoonhoven <@t> yahoo.com (Robert Schoonhoven) Date: Thu Mar 7 12:00:41 2013 Subject: [Histonet] problem with DAB staining In-Reply-To: <5138D352.1070004@siumed.edu> References: <5138D352.1070004@siumed.edu> Message-ID: <1362679234.87227.YahooMailNeo@web126203.mail.ne1.yahoo.com> We would need to see the entire set of steps as there?may be?a variety of causes. Robert Schoonhoven, HT/HTL (ASCP) ________________________________ From: Nicole Cosenza To: histonet@lists.utsouthwestern.edu Sent: Thursday, March 7, 2013 12:50 PM Subject: [Histonet] problem with DAB staining Hello all: I recently did IHC on paraffin embedded slides via the DAB method. Upon addition of the DAB, the entire section turned brown. I know this is not true staining, as I don't expect a high yield of my protein of interest. Any suggestions as to why this happened and what to do about it? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tahseen <@t> brain.net.pk Thu Mar 7 12:20:33 2013 From: tahseen <@t> brain.net.pk (tahseen) Date: Thu Mar 7 12:20:54 2013 Subject: [Histonet] problem with DAB staining In-Reply-To: <5138D352.1070004@siumed.edu> References: <5138D352.1070004@siumed.edu> Message-ID: <5d908a6d1593909336ccb5488917c524@brain.net.pk> Section should not dry in any stapes. Tahseen On 2013-03-07 22:50, Nicole Cosenza wrote: > Hello all: > > I recently did IHC on paraffin embedded slides via the DAB method. > Upon addition of the DAB, the entire section turned brown. I know > this > is not true staining, as I don't expect a high yield of my protein of > interest. Any suggestions as to why this happened and what to do > about > it? > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rgeske_2000 <@t> yahoo.com Thu Mar 7 12:39:41 2013 From: rgeske_2000 <@t> yahoo.com (Rob Geske) Date: Thu Mar 7 12:39:44 2013 Subject: [Histonet] problem with DAB staining In-Reply-To: <5138D352.1070004@siumed.edu> Message-ID: <1362681581.49475.YahooMailClassic@web160505.mail.bf1.yahoo.com> Did you use a serum block ahead of your primary antibody ?? If yes, what species was it derived from? In what species was your primary antibody generated ? --- On Thu, 3/7/13, Nicole Cosenza wrote: From: Nicole Cosenza Subject: [Histonet] problem with DAB staining To: histonet@lists.utsouthwestern.edu Date: Thursday, March 7, 2013, 11:50 AM Hello all: I recently did IHC on paraffin embedded slides via the DAB method. Upon addition of the DAB, the entire section turned brown. I know this is not true staining, as I don't expect a high yield of my protein of interest. Any suggestions as to why this happened and what to do about it? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From renafail2 <@t> gmail.com Thu Mar 7 13:01:13 2013 From: renafail2 <@t> gmail.com (Rena Fail) Date: Thu Mar 7 13:01:17 2013 Subject: [Histonet] problem with DAB staining In-Reply-To: <1362681581.49475.YahooMailClassic@web160505.mail.bf1.yahoo.com> References: <5138D352.1070004@siumed.edu> <1362681581.49475.YahooMailClassic@web160505.mail.bf1.yahoo.com> Message-ID: The most likey reasons are the tissue dried out at some point or the slide was not adequately rinsed between steps Rena Fail On Thu, Mar 7, 2013 at 1:39 PM, Rob Geske wrote: > Did you use a serum block ahead of your primary antibody ? If yes, what > species was it derived from? > > In what species was your primary antibody generated ? > > > > --- On Thu, 3/7/13, Nicole Cosenza wrote: > > From: Nicole Cosenza > Subject: [Histonet] problem with DAB staining > To: histonet@lists.utsouthwestern.edu > Date: Thursday, March 7, 2013, 11:50 AM > > Hello all: > > I recently did IHC on paraffin embedded slides via the DAB method. Upon > addition of the DAB, the entire section turned brown. I know this is not > true staining, as I don't expect a high yield of my protein of interest. > Any suggestions as to why this happened and what to do about it? > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From kgrobert <@t> rci.rutgers.edu Thu Mar 7 13:32:23 2013 From: kgrobert <@t> rci.rutgers.edu (kgrobert@rci.rutgers.edu) Date: Thu Mar 7 13:32:27 2013 Subject: [Histonet] Microtome upgrade, planning stages... Message-ID: We are starting to look into upgrading to "higher-end", but used, microtomes. What we have is still working, but the writing is on the wall for them in terms of repair/parts. Which brands/models are considered "higher-end" in the used market these days? (Leica is already on my list, but which models?) Thanks so much! Kathleen Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology & Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (848) 445-1443 FAX (732) 445-6905 From rjbuesa <@t> yahoo.com Thu Mar 7 14:04:32 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Mar 7 14:04:37 2013 Subject: [Histonet] Microtome upgrade, planning stages... In-Reply-To: References: Message-ID: <1362686672.28850.YahooMailNeo@web163101.mail.bf1.yahoo.com> Add "another Leica" to your list Ren? J, From: "kgrobert@rci.rutgers.edu" To: histonet Sent: Thursday, March 7, 2013 2:32 PM Subject: [Histonet] Microtome upgrade, planning stages... We are starting to look into upgrading to "higher-end", but used, microtomes.? What we have is still working, but the writing is on the wall for them in terms of repair/parts. Which brands/models are considered "higher-end" in the used market these days?? (Leica is already on my list, but which models?) Thanks so much! Kathleen Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology & Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (848) 445-1443 FAX (732) 445-6905 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BZIMMERM <@t> gru.edu Thu Mar 7 14:29:33 2013 From: BZIMMERM <@t> gru.edu (Zimmerman, Billie) Date: Thu Mar 7 14:29:39 2013 Subject: [Histonet] Kudos Message-ID: <7B3DEB32E69C034EACB479059C5DE3FF75A2FE@EX-MLB-03.ad.georgiahealth.edu> Thanks to everyone for your suggestions and protocols for the IDH1 antibody. It helped immensely and now I don't have to beat my head against the wall anymore! Billie Zimmerman MT(ASCP)QIHC Laboratory Clinical Operations Mgr. BF 117 706-721-5617/3630 Fax: 706-434-4883 Augusta State University and Georgia Health Sciences University have consolidated to become Georgia Regents University. Effective January 9, 2013, my email address has changed to BZIMMERM@gru.edu. Please update your address book to reflect this change. From epollock <@t> ucalgary.ca Thu Mar 7 15:24:42 2013 From: epollock <@t> ucalgary.ca (Betty Pollock) Date: Thu Mar 7 15:25:02 2013 Subject: [Histonet] SOP numbering for whole lab In-Reply-To: <1362436350.93849.YahooMailClassic@web161905.mail.bf1.yahoo.com> References: <1362436350.93849.YahooMailClassic@web161905.mail.bf1.yahoo.com> Message-ID: <4AA1FAB9B20A064C8FE823293DE8AD741CCA0D4BC8@EXMB05.admin.ad.ucalgary.ca> Our histology SOPs start with H- (to distinguish them from other area SOPs) then each is assigned a unique chronological 4 digit ID number followed by the version number. For example: H-0001.00 where 0001 is the number for a specific SOP and .00 refers to the original version. The number for the first revision of this SOP would be H-0001.01. It's simple and every SOP and revision has a unique number. We keep master lists of the SOPs and the SOPs are under document control as well so each has its own history file. Betty Pollock Diagnostic Services Unit Manager Faculty of Veterinary Medicine University of Calgary Calgary, AB, Canada T3R 1J3 Tel: 403-220-2806 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl Sent: March 4, 2013 3:33 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] SOP numbering for whole lab Help?? What kind of formula do you use when numbering and coding your SOPs for the lab?? Is there an easy way to keep them sorted and in line with all the regulatory bodies to help on inspection days? ? Any suggestions welcome--we're starting from scratch. ? Cheryl ? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kgrobert <@t> rci.rutgers.edu Thu Mar 7 15:41:24 2013 From: kgrobert <@t> rci.rutgers.edu (kgrobert@rci.rutgers.edu) Date: Thu Mar 7 15:41:28 2013 Subject: [Histonet] Has anybody ever used... Message-ID: <29f1a4fe7da34c2f0652a378376f1435.squirrel@webmail.rci.rutgers.edu> Leica's CoolClamp, or Thermo's Cool Cut? These sound like a possible good solution for the inevitable days when the aging AC plant here can't keep up with the summer heat. Thanks so much! Kathleen Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology & Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (848) 445-1443 FAX (732) 445-6905 From carlos.mora <@t> chem.ethz.ch Fri Mar 8 03:44:20 2013 From: carlos.mora <@t> chem.ethz.ch (Mora Carlos) Date: Fri Mar 8 03:44:30 2013 Subject: [Histonet] Histology protocol for Sectioning of Drosophila melanogaster Message-ID: <1DE0B7D63F28C34EA2B9C93553AD52DB16E81C06@MBX10.d.ethz.ch> Dear all I am searching for a histology protocol for whole fly sectioning for subsequent confocal fluorescence microscopy. Is there anyone who has ever done such an experiment and can provide a histology protocol? Thank you very much for your reply. Best wishes Carlos ________________________________ ETH Zurich Carlos Mora, PhD student Institute for Chemical and Bio-Engineering HCI E 112 Wolfgang-Pauli-Str. 10 CH-8093 Zurich SWITZERLAND carlos.mora@chem.ethz.ch www.fml.ethz.ch +41 44 632 30 54 phone +41 44 633 15 71 fax From mw <@t> personifysearch.com Fri Mar 8 08:22:09 2013 From: mw <@t> personifysearch.com (Matt Ward) Date: Fri Mar 8 08:22:22 2013 Subject: [Histonet] New Position Alert - Antibody Staining Consultant Message-ID: <856fe465ebd2ab575a97d189598fd380@mail.gmail.com> Good morning Histonet! Our client has recently opened a new expansion histology/antibody opportunity and I wanted to reach out regarding a few specifics and see if anyone would be interested in learning more. Our client is a global leader in Histology and IHC has recently opened a new expansion opportunity in the Midwest Region to focus on driving IHC Reagent and Antibody sales. We are searching for a histology professional who has a strong background in IHC and antibodies that would be interested in working in a team sales/consultant environment. Though sales experience is a plus, this position does not require previous sales experience and this would be outstanding opportunity for someone looking to break into sales that has a strong histology background. The ideal location for the position would be in the greater Minneapolis area, however we will consider candidates in the region (MN, ND, SD, IA, NE, KS). The position offer a strong package including a base salary, competitive commission structure, car allowance, gas card, cell phone, laptop computer, and full benefits. If you or anyone you may know would be interested in the position, please contact me directly mw@personifysearch.com or my colleague Courtney Harris at cah@personifysearch.com to learn more. Regards, Matt Ward *Account Executive* *Personify* 5020 Weston Parkway Suite 315 Cary NC 27513 (Tel) 800.875.6188 direct ext 103 (Fax) 919.460.0642 www.personifysearch.com From rmweber113 <@t> comcast.net Fri Mar 8 10:08:08 2013 From: rmweber113 <@t> comcast.net (rmweber113@comcast.net) Date: Fri Mar 8 10:08:29 2013 Subject: [Histonet] Leica Bond Max Message-ID: <2059431589.182624.1362758888218.JavaMail.root@sz0187a.westchester.pa.mail.comcast.net> Good Morning,? I have a question about the Leica Bond Max reagents.? Has anyone ever heard of purchasing the reagents from a 3rd party rather than from Leica ?? If so, what is the company's contact info. Thanks for your help. Marilynn Weber H.T.( ASCP ) QIHC Coastal Pathology Consulting Services LLC 732 814-1170 fax 267 722-8308 From BZIMMERM <@t> gru.edu Fri Mar 8 11:20:38 2013 From: BZIMMERM <@t> gru.edu (Zimmerman, Billie) Date: Fri Mar 8 11:20:49 2013 Subject: [Histonet] Georgia Society for Histology Symposium April 12 - 14, 2013 Message-ID: <7B3DEB32E69C034EACB479059C5DE3FF75A7CD@EX-MLB-03.ad.georgiahealth.edu> Please join us on Jekyll Island for the 40th anniversary celebration of the Georgia Society for Histotechnology. For an all-inclusive price of $135 you can obtain up to 15 CEU's through the National Society for Histotechnology. This is an affordable opportunity to network, learn from knowledgeable speakers, and visit the exhibit/vendor area. Please go to our website for symposium registration and room accommodations at Oceanside Inns and Suites. (Oceanside will honor our rate as long as rooms are available) We are only 4 short weeks away from our celebration. Jekyll Island is a historical state park featuring family fun, affordable adventures, and spectacular sunrises and sunsets. Come and leave your footprint on the beach and catch the "wave of the future in Histotechnology"! Warm regards, Wanda K. Simons HT(ASCP) GSH President www.histosearch.com/gsh/ (FREE MEMBERSHIP) WS/bz Augusta State University and Georgia Health Sciences University have consolidated to become Georgia Regents University. Effective January 9, 2013, my email address has changed to BZIMMERM@gru.edu. Please update your address book to reflect this change. From SWeaver <@t> tvmdl.tamu.edu Fri Mar 8 12:33:09 2013 From: SWeaver <@t> tvmdl.tamu.edu (Weaver, Stephanie) Date: Fri Mar 8 12:33:18 2013 Subject: [Histonet] RE: Histonet Digest, Vol 112, Issue 9 In-Reply-To: <20130308180544.23D4A21419F@barracuda.tvmdl.tamu.edu> References: <20130308180544.23D4A21419F@barracuda.tvmdl.tamu.edu> Message-ID: <1E7EE2744895574CB92A6B0CE1FBEDBB0103EA@tvmdlmail01.lab.tvmdl.edu> We purchased a Leica RM2125RMS manual rotary microtome new from Leica less than a year ago and you may want to be aware of the issues that we are having. We purchased it for multiple reasons, including the excellent reputation of Leica microtomes and the great sectioning of tissues (even bone) that we experienced during the demo. It still cuts great sections but I am not pleased with this purchase. We have replaced the cassette clamp at least 6 times since we've purchased it because the solid steel level to pull to open the cassette clamp breaks. Each new cassette clamp seems to last less time than the last. We are still under warranty so they have replaced this part for free every time and they are always kind when I tell them it broke again, but this is just not acceptable. We have been told that it is a known manufacturing problem and that they are working on a resolution but I would still be wary since this is obviously not fixed yet. The body of the microtome is also already showing discoloration and signs of corrosion but since these parts do not move and are not under stress it does not seem to be affecting the microtome. At this point I don't think I would ever buy Leica again, but I know that a lot of people have loved their equipment and have had very good longevity out of it. Good luck, Stephanie Weaver, HT (ASCP) From: "kgrobert@rci.rutgers.edu" To: histonet Sent: Thursday, March 7, 2013 2:32 PM Subject: [Histonet] Microtome upgrade, planning stages... We are starting to look into upgrading to "higher-end", but used, microtomes.? What we have is still working, but the writing is on the wall for them in terms of repair/parts. Which brands/models are considered "higher-end" in the used market these days?? (Leica is already on my list, but which models?) Thanks so much! Kathleen Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology & Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (848) 445-1443 FAX (732) 445-6905 From ryaskovich <@t> dir.nidcr.nih.gov Fri Mar 8 13:00:33 2013 From: ryaskovich <@t> dir.nidcr.nih.gov (Yaskovich, Ruth A (NIH/NIDCR) [E]) Date: Fri Mar 8 13:01:01 2013 Subject: [Histonet] RE: Histonet Digest, Vol 112, Issue 9 In-Reply-To: <1E7EE2744895574CB92A6B0CE1FBEDBB0103EA@tvmdlmail01.lab.tvmdl.edu> References: <20130308180544.23D4A21419F@barracuda.tvmdl.tamu.edu> <1E7EE2744895574CB92A6B0CE1FBEDBB0103EA@tvmdlmail01.lab.tvmdl.edu> Message-ID: <502052F0C384624FA5F2EF37D686AACC0F39F6DB@MLBXV06.nih.gov> Stephanie, This good to know because I'm considering a new microtome. Ruth N.I.H. -----Original Message----- From: Weaver, Stephanie [mailto:SWeaver@tvmdl.tamu.edu] Sent: Friday, March 08, 2013 1:33 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Histonet Digest, Vol 112, Issue 9 We purchased a Leica RM2125RMS manual rotary microtome new from Leica less than a year ago and you may want to be aware of the issues that we are having. We purchased it for multiple reasons, including the excellent reputation of Leica microtomes and the great sectioning of tissues (even bone) that we experienced during the demo. It still cuts great sections but I am not pleased with this purchase. We have replaced the cassette clamp at least 6 times since we've purchased it because the solid steel level to pull to open the cassette clamp breaks. Each new cassette clamp seems to last less time than the last. We are still under warranty so they have replaced this part for free every time and they are always kind when I tell them it broke again, but this is just not acceptable. We have been told that it is a known manufacturing problem and that they are working on a resolution but I would still be wary since this is obviously not fixed yet. The body of the microtome is also already showing discoloration and signs of corrosion but since these parts do not move and are not under stress it does not seem to be affecting the microtome. At this point I don't think I would ever buy Leica again, but I know that a lot of people have loved their equipment and have had very good longevity out of it. Good luck, Stephanie Weaver, HT (ASCP) From: "kgrobert@rci.rutgers.edu" To: histonet Sent: Thursday, March 7, 2013 2:32 PM Subject: [Histonet] Microtome upgrade, planning stages... We are starting to look into upgrading to "higher-end", but used, microtomes.? What we have is still working, but the writing is on the wall for them in terms of repair/parts. Which brands/models are considered "higher-end" in the used market these days?? (Leica is already on my list, but which models?) Thanks so much! Kathleen Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology & Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (848) 445-1443 FAX (732) 445-6905 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bouchalr <@t> wvuhealthcare.com Fri Mar 8 13:07:31 2013 From: bouchalr <@t> wvuhealthcare.com (Bouchal, Rena L) Date: Fri Mar 8 13:07:40 2013 Subject: [Histonet] RE: Histonet Digest, Vol 112, Issue 9 In-Reply-To: <1E7EE2744895574CB92A6B0CE1FBEDBB0103EA@tvmdlmail01.lab.tvmdl.edu> References: <20130308180544.23D4A21419F@barracuda.tvmdl.tamu.edu> <1E7EE2744895574CB92A6B0CE1FBEDBB0103EA@tvmdlmail01.lab.tvmdl.edu> Message-ID: <3F978C8467623541BA99D7EBFCF4BBD5013FC03D@NT-EXMB1.wvuh.wvuhs.com> We have had the same experiences with the clamps. At one point we were told we had to purchase a complete $2000 head when one of our clamps went. We "got ugly" and they gave it to us for free, but it was not pleasant. We have several Leica's and I am going to look at a Microm this year because of these problems. No one has ever admitted to us that there is a "known manufacturing problem". Its too bad because other than that we have been quite pleased with the units. I just don't want to fight with the company routinely over clamps. Please note that my email address as of Jan 3, 2011 is bouchalr@wvuhealthcare.com . Please make the appropriate changes in your address book. Rena Bouchal, M.S. Anatomic Pathology Manager West Virginia University Hospitals 304-293-7765 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weaver, Stephanie Sent: Friday, March 08, 2013 1:33 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Histonet Digest, Vol 112, Issue 9 We purchased a Leica RM2125RMS manual rotary microtome new from Leica less than a year ago and you may want to be aware of the issues that we are having. We purchased it for multiple reasons, including the excellent reputation of Leica microtomes and the great sectioning of tissues (even bone) that we experienced during the demo. It still cuts great sections but I am not pleased with this purchase. We have replaced the cassette clamp at least 6 times since we've purchased it because the solid steel level to pull to open the cassette clamp breaks. Each new cassette clamp seems to last less time than the last. We are still under warranty so they have replaced this part for free every time and they are always kind when I tell them it broke again, but this is just not acceptable. We have been told that it is a known manufacturing problem and that they are working on a resolution but I would still be wary since this is obviously not fixed yet. The body of the microtome is also already showing discoloration and signs of corrosion but since these parts do not move and are not under stress it does not seem to be affecting the microtome. At this point I don't think I would ever buy Leica again, but I know that a lot of people have loved their equipment and have had very good longevity out of it. Good luck, Stephanie Weaver, HT (ASCP) From: "kgrobert@rci.rutgers.edu" To: histonet Sent: Thursday, March 7, 2013 2:32 PM Subject: [Histonet] Microtome upgrade, planning stages... We are starting to look into upgrading to "higher-end", but used, microtomes.? What we have is still working, but the writing is on the wall for them in terms of repair/parts. Which brands/models are considered "higher-end" in the used market these days?? (Leica is already on my list, but which models?) Thanks so much! Kathleen Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology & Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (848) 445-1443 FAX (732) 445-6905 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BZIMMERM <@t> gru.edu Fri Mar 8 13:50:13 2013 From: BZIMMERM <@t> gru.edu (Zimmerman, Billie) Date: Fri Mar 8 13:50:42 2013 Subject: [Histonet] EGFRvIII Message-ID: <7B3DEB32E69C034EACB479059C5DE3FF75A89F@EX-MLB-03.ad.georgiahealth.edu> Our pathologists are requesting an antibody EGFRvIII. Is there anyone using this antibody on the Ventana Ultra or XT? Thanks in advance for your help. Billie Zimmerman MT(ASCP)QIHC 706-721-5617/3630 Augusta State University and Georgia Health Sciences University have consolidated to become Georgia Regents University. Effective January 9, 2013, my email address has changed to BZIMMERM@gru.edu. Please update your address book to reflect this change. From Maxim_71 <@t> mail.ru Fri Mar 8 22:46:35 2013 From: Maxim_71 <@t> mail.ru (Maxim Peshkov) Date: Fri Mar 8 22:46:44 2013 Subject: [Histonet] Microtome upgrade, planning stages Message-ID: <78240537.20130309084635@mail.ru> I tried some different microtomes (Leica, Microm, Thermo Shandon, Sakura), as sledge as rotary types. My choise is Leica RM2245. Leica RM2245 is semi automatic? and RM2255 or RM2265 are full automatic models. I like RM2245 because this model have a "rock" mode, when operator does for a section only 1/4 rotation. It is very ergonomic! Shandon Finesse ME also have "rock" mode, but I have not happy with it. Leica RM series have only one disadvantage - two different type for disposable blade holder (for low and high profile, but not universal). Buy both type holders. RM2245 cheaper than 55 or 65. Series 2125 is the same as Microm 325. I do not try Leica Cool Clamp. Hope this help. Maxim Peshkov Russia, Taganrog. From Steven.Weston <@t> utas.edu.au Fri Mar 8 23:55:31 2013 From: Steven.Weston <@t> utas.edu.au (Steven Weston) Date: Fri Mar 8 23:55:41 2013 Subject: [Histonet] re problem with DAB staining Message-ID: <7B808A2E6BDDBD4EA4FA6395FF7BED2B3D944F41@MBXSBYN2.utas.ad.internal> Another suggestion is that your sections may have dried out sometime after the antibodies have been added and before you put on the DAB. regards steve weston lab manager resp research group MRI From passorin <@t> yahoo.com Sat Mar 9 01:48:11 2013 From: passorin <@t> yahoo.com (Pasca Sorin) Date: Sat Mar 9 01:48:19 2013 Subject: [Histonet] (no subject) Message-ID: Sorin-Aurelian PASCA, PhD,DVM Pathological Anatomy and Forensic Medicine Faculty of Veterinary Medicine ?University of Applied Life Sciences and Environment? 8, Al. M. Sadoveanu, 700489, Iasi Romania Mobil: 0040740174130 Office: 0040232407335 e-mail: passorin@yahoo.com spasca@uaiasi.ro From nelsonrnch <@t> verizon.net Sat Mar 9 02:58:43 2013 From: nelsonrnch <@t> verizon.net (PATTI NELSON HT(ASCP)) Date: Sat Mar 9 02:58:49 2013 Subject: [Histonet] *HISTOTECHNICIAN POSITION IN LONG BEACH, CALIF.* Message-ID: <1362819523.71910.YahooMailNeo@web84504.mail.ne1.yahoo.com> To All, Immediate position and great opportunity for a Histotechnician?with MOH's experience?in Long Beach,?Ca. Derm Lab?is looking for a certified HT or HTL to run their?laboratory. Candidate must be ASCP certified and CLIA certified to perform gross dissection, Responsibilities would include the following: Maintenance of policies and procedures to State/CLIA standards, leading lab through State/CLIA inspection, maintenance?,?QA?and QC?for equipment and routine histology duties. Such as all of the histology duties from patient accessioning, grossing, embedding, cutting, staining to cover-slipping. This is a?full time?position that offers a competitive rate and flexible hours.? Interested applicants should contact me at your earliest convenience. Please privately in-box me your resumes/CV. ? BEST REGARDS, ? PATTI RUBEN-NELSON? H.T.(ASCP) PNP LABORATORY CONSULTANTS SUPERVISOR/DGC P.O. BOX 412 CABAZON, CA. 92230 cell (909) 841-9761 nelsonrnch@verizon.net ? CONFIDENTIALITY NOTICE: This message and any included attachments are from Patti Nelson, PNP Laboratory Consultants? ?and are intended only for the addressee.? The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or exemption from disclosure under applicable law.? Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful.? If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call? 909-841-9761. From ssegal2 <@t> slu.edu Sat Mar 9 13:22:20 2013 From: ssegal2 <@t> slu.edu (Salomao Segal) Date: Sat Mar 9 13:22:29 2013 Subject: [Histonet] microscope camera Message-ID: I have a Zeiss Universal microscope with a C mount but no camera. My question is: what is the best microscope camera that little money can buy? Any suggestions are most appreciated. Best -- Solomon Segal, M.D. Assistant Professor of Anatomy in Surgery Center for Anatomical Science and Education (CASE) Department of Surgery School of Medicine Saint Louis University 1402 South Grand Blvd. Schwitalla Hall - 3rd Floor - M310 Saint Louis, MO, 63104 office: 314 977 8023 laboratory: 314 977 8080 CASE: 314 977 8027 FAX: 314 977 5127 e-mail: ssegal2@slu.edu http://medschool.slu.edu/anatomy/ http://slu.academia.edu/SolomonSegal https://sites.google.com/a/slu.edu/segal-laboratory/ https://sites.google.com/a/slu.edu/dr-segal-s-clinical-anatomy-website/ From mwfolsom <@t> swcp.com Sat Mar 9 21:37:10 2013 From: mwfolsom <@t> swcp.com (Michael W. Folsom) Date: Sat Mar 9 21:37:46 2013 Subject: [Histonet] microscope camera In-Reply-To: References: Message-ID: <513BFFE6.9070802@swcp.com> Actually, if you already have a C-mount on your scope there are some inexpensive options. However, you might only capture part of your field of view depending on how big the chip is in your camera. Question does the c-mount on top of your scope have a part number? Is there any kind of lense in it or is it a tube connected to the head? Re: the camera - on Ebay you can find them starting at a bit above $100 - just depends on how many mega pixels you want. Remember depending on the magnification you can get into empty resolution pretty quickly. Mike On 03/09/2013 12:22 PM, Salomao Segal wrote: > I have a Zeiss Universal microscope with a C mount but no camera. > > My question is: what is the best microscope camera that little money can > buy? > > Any suggestions are most appreciated. > > Best > From max_histo_00 <@t> yahoo.it Sun Mar 10 03:39:42 2013 From: max_histo_00 <@t> yahoo.it (Massimo) Date: Sun Mar 10 04:35:53 2013 Subject: [Histonet] microscope camera In-Reply-To: References: Message-ID: <1362904782.77902.YahooMailNeo@web172003.mail.ir2.yahoo.com> Hi, on the phototube of the "Lomo" tri-ocular head of my microscope I use a Nikon Coolpix P310 Camera without "C" adaper. The phototube mounts a "Leitz" eyepiece 10x for photography (red dot) with a high exit eyepoint and a standard outside diameter barrel of 23.2 mm. The eyepiece is placed into an aluminum cylinder with an external diameter of 50.0 mm. and fixed by a plastic screw. The camera is hold on the cylinder by an "Universal Digiscoping Adapter" (diameter range: 43 - 65 mm.) brand "Vortex", which allows the movement of the camera in the X - Y directions. You can watch at the assembled system and some results on this folder link: http://www.mediafire.com/?qpo6i34ibh8o7 COST of the Nikon P310 camera in Italy on 29 May 2012: 350.00 Euro If you want to keep your "C" mount adaper you can choice among Nikon Coolpix cameras: 900, 995 and 4500. They are quite obsolete with cheap costs and you can still find something on "eBay". Despite its low cost they give good results. Kind Regards Massimo Tosi "A humble Chemical Engineer who loves Histology" ________________________________ Da: Salomao Segal A: histonet@lists.utsouthwestern.edu Inviato: Sabato 9 Marzo 2013 20:22 Oggetto: [Histonet] microscope camera I have a Zeiss Universal microscope with a C mount but no camera. My question is: what is the best microscope camera that little money can buy? Any suggestions are most appreciated. Best -- Solomon Segal, M.D. Assistant Professor of Anatomy in Surgery Center for Anatomical Science and Education (CASE) Department of Surgery School of Medicine Saint Louis University 1402 South Grand Blvd. Schwitalla Hall - 3rd Floor - M310 Saint Louis, MO, 63104 office: 314 977 8023 laboratory: 314 977 8080 CASE: 314 977 8027 FAX: 314 977 5127 e-mail: ssegal2@slu.edu http://medschool.slu.edu/anatomy/ http://slu.academia.edu/SolomonSegal https://sites.google.com/a/slu.edu/segal-laboratory/ https://sites.google.com/a/slu.edu/dr-segal-s-clinical-anatomy-website/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mayallf <@t> wave.co.nz Sun Mar 10 07:12:58 2013 From: mayallf <@t> wave.co.nz (Dr Mayall) Date: Sun Mar 10 07:13:06 2013 Subject: [Histonet] The Free Diagnostic Pathology Software Project from the UK - The third version of this free software is now being shared. Message-ID: The Free Diagnostic Pathology Software Project is sharing the third version of its free open source cellular pathology reporting software. You can try it online and download it at the project's website: www.FreeDP.org You are free to use it, change it and share it with others under the GPL3 open source license. The project's website has been visited by pathologists from over 55 countries. One in five visitors chooses to download a copy of the software for use on their own computer. It can be used with Windows of Apple OS. It is being used at hundreds of sites around the World. Version 1.1 of the software was distributed online in April 2012 and Version 2.1 in September 2012. During 2012 we developed proformas for more tumour types including ones based on the recently published The International Collaboration on Cancer Reporting (ICCR) proformas for endometrial carcinoma and invasive melanoma. The latest version, published in March 2013 includes improved breast carcinoma and colorectal carcinoma proformas together with improved PDF printing and some debugging. Over the next year we hope to incorporate more cancer proformas, and will release an updated version towards the end of the year, via the website above. I would be very for any feed back regarding bugs or ideas for enhancements. Fred Mayall Consultant Histopathologist, Taunton and Somerset NHS Trust From tkngflght <@t> yahoo.com Sun Mar 10 14:12:18 2013 From: tkngflght <@t> yahoo.com (Cheryl) Date: Sun Mar 10 14:12:23 2013 Subject: [Histonet] Happy Histotech Day! Message-ID: <1362942738.16061.YahooMailClassic@web161904.mail.bf1.yahoo.com> Hoping you get this from EVERYONE at work this week-- ? As alway, Histologists are a cut above !! ? Happy happy! ? Cheryl ? Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab by helping one GREAT?Tech at a time.? 281.852.9457?Office 800.756.3309?Phone & Fax? admin@fullstaff.org Sign up for the FREE?newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please?include your name and specialty in the body of the email. From Susan.Walzer <@t> HCAHealthcare.com Mon Mar 11 02:11:23 2013 From: Susan.Walzer <@t> HCAHealthcare.com (Susan.Walzer@HCAHealthcare.com) Date: Mon Mar 11 02:11:31 2013 Subject: [Histonet] RE: Histonet Digest, Vol 112, Issue 9 In-Reply-To: <3F978C8467623541BA99D7EBFCF4BBD5013FC03D@NT-EXMB1.wvuh.wvuhs.com> References: <20130308180544.23D4A21419F@barracuda.tvmdl.tamu.edu> <1E7EE2744895574CB92A6B0CE1FBEDBB0103EA@tvmdlmail01.lab.tvmdl.edu> <3F978C8467623541BA99D7EBFCF4BBD5013FC03D@NT-EXMB1.wvuh.wvuhs.com> Message-ID: <4BF03F5404EBDE409AF9232DA74B9DED2DFF7B2248@FWDCWPMSGCMS09.hca.corpad.net> The problem seems to be that the clamp screw handle is welded on and that is the weakness. On the old tall Leicas (does anyone remember them?) the screw-in lever was solid and we never had this problem. Also the springs get rusty inside the clamps probably from corrosive surface decal. It seems to be an ongoing problem. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bouchal, Rena L Sent: Friday, March 08, 2013 2:08 PM To: 'Weaver, Stephanie'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Histonet Digest, Vol 112, Issue 9 We have had the same experiences with the clamps. At one point we were told we had to purchase a complete $2000 head when one of our clamps went. We "got ugly" and they gave it to us for free, but it was not pleasant. We have several Leica's and I am going to look at a Microm this year because of these problems. No one has ever admitted to us that there is a "known manufacturing problem". Its too bad because other than that we have been quite pleased with the units. I just don't want to fight with the company routinely over clamps. Please note that my email address as of Jan 3, 2011 is bouchalr@wvuhealthcare.com . Please make the appropriate changes in your address book. Rena Bouchal, M.S. Anatomic Pathology Manager West Virginia University Hospitals 304-293-7765 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weaver, Stephanie Sent: Friday, March 08, 2013 1:33 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Histonet Digest, Vol 112, Issue 9 We purchased a Leica RM2125RMS manual rotary microtome new from Leica less than a year ago and you may want to be aware of the issues that we are having. We purchased it for multiple reasons, including the excellent reputation of Leica microtomes and the great sectioning of tissues (even bone) that we experienced during the demo. It still cuts great sections but I am not pleased with this purchase. We have replaced the cassette clamp at least 6 times since we've purchased it because the solid steel level to pull to open the cassette clamp breaks. Each new cassette clamp seems to last less time than the last. We are still under warranty so they have replaced this part for free every time and they are always kind when I tell them it broke again, but this is just not acceptable. We have been told that it is a known manufacturing problem and that they are working on a resolution but I would still be wary since this is obviously not fixed yet. The body of the microtome is also already showing discoloration and signs of corrosion but since these parts do not move and are not under stress it does not seem to be affecting the microtome. At this point I don't think I would ever buy Leica again, but I know that a lot of people have loved their equipment and have had very good longevity out of it. Good luck, Stephanie Weaver, HT (ASCP) From: "kgrobert@rci.rutgers.edu" To: histonet Sent: Thursday, March 7, 2013 2:32 PM Subject: [Histonet] Microtome upgrade, planning stages... We are starting to look into upgrading to "higher-end", but used, microtomes.? What we have is still working, but the writing is on the wall for them in terms of repair/parts. Which brands/models are considered "higher-end" in the used market these days?? (Leica is already on my list, but which models?) Thanks so much! Kathleen Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology & Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (848) 445-1443 FAX (732) 445-6905 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Susan.Walzer <@t> HCAHealthcare.com Mon Mar 11 02:12:38 2013 From: Susan.Walzer <@t> HCAHealthcare.com (Susan.Walzer@HCAHealthcare.com) Date: Mon Mar 11 02:12:45 2013 Subject: [Histonet] RE: Histonet Digest, Vol 112, Issue 9 In-Reply-To: <502052F0C384624FA5F2EF37D686AACC0F39F6DB@MLBXV06.nih.gov> References: <20130308180544.23D4A21419F@barracuda.tvmdl.tamu.edu> <1E7EE2744895574CB92A6B0CE1FBEDBB0103EA@tvmdlmail01.lab.tvmdl.edu> <502052F0C384624FA5F2EF37D686AACC0F39F6DB@MLBXV06.nih.gov> Message-ID: <4BF03F5404EBDE409AF9232DA74B9DED2DFF7B2249@FWDCWPMSGCMS09.hca.corpad.net> PS.. Even with the clamp problem I think the Leicas are superior. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Yaskovich, Ruth A (NIH/NIDCR) [E] Sent: Friday, March 08, 2013 2:01 PM To: 'Weaver, Stephanie'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Histonet Digest, Vol 112, Issue 9 Stephanie, This good to know because I'm considering a new microtome. Ruth N.I.H. -----Original Message----- From: Weaver, Stephanie [mailto:SWeaver@tvmdl.tamu.edu] Sent: Friday, March 08, 2013 1:33 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Histonet Digest, Vol 112, Issue 9 We purchased a Leica RM2125RMS manual rotary microtome new from Leica less than a year ago and you may want to be aware of the issues that we are having. We purchased it for multiple reasons, including the excellent reputation of Leica microtomes and the great sectioning of tissues (even bone) that we experienced during the demo. It still cuts great sections but I am not pleased with this purchase. We have replaced the cassette clamp at least 6 times since we've purchased it because the solid steel level to pull to open the cassette clamp breaks. Each new cassette clamp seems to last less time than the last. We are still under warranty so they have replaced this part for free every time and they are always kind when I tell them it broke again, but this is just not acceptable. We have been told that it is a known manufacturing problem and that they are working on a resolution but I would still be wary since this is obviously not fixed yet. The body of the microtome is also already showing discoloration and signs of corrosion but since these parts do not move and are not under stress it does not seem to be affecting the microtome. At this point I don't think I would ever buy Leica again, but I know that a lot of people have loved their equipment and have had very good longevity out of it. Good luck, Stephanie Weaver, HT (ASCP) From: "kgrobert@rci.rutgers.edu" To: histonet Sent: Thursday, March 7, 2013 2:32 PM Subject: [Histonet] Microtome upgrade, planning stages... We are starting to look into upgrading to "higher-end", but used, microtomes.? What we have is still working, but the writing is on the wall for them in terms of repair/parts. Which brands/models are considered "higher-end" in the used market these days?? (Leica is already on my list, but which models?) Thanks so much! Kathleen Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology & Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (848) 445-1443 FAX (732) 445-6905 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From one_angel_secret <@t> yahoo.com Mon Mar 11 10:38:49 2013 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Mon Mar 11 10:38:55 2013 Subject: [Histonet] Fw: news Message-ID: <1363016329.73457.YahooMailNeo@web161602.mail.bf1.yahoo.com> http://www.britvoice.co.uk/wwvc/woszquloax/hkjmnqu=bvdzq From relia1 <@t> earthlink.net Mon Mar 11 11:04:40 2013 From: relia1 <@t> earthlink.net (Pam Barker) Date: Mon Mar 11 11:04:45 2013 Subject: [Histonet] Happy Histotechnology Professionals Day from RELIA Solutions and Pam Barker. Also a Hot Histology Job Alert that I want to share with you. Message-ID: <016b01ce1e72$23ae65e0$6b0b31a0$@earthlink.net> Hi Histonetters!! Happy Histotechnology Professionals Day - Since it landed on Sunday I hope you get to celebrate all week long! I also have a new opportunity to tell you about: Anatomic Pathology Supervisor - Atlanta, GA RELIA Solutions has been engaged to assist in the recruitment of an Anatomic Pathology Supervisor for a prestigious institution located in Atlanta, GA. ASCP HT/HTL or CT, 5 years lab experience and 4 years of supervisory experience are required for this position. My client offers a competitive salary, excellent benefits, relocation assistance, a stable work environment and the opportunity to work at one of the most prestigious facilities in the Southeast U.S. If this is the right job for you RELIA can make it happen! For more information please contact Pam Barker at relia1@earthlink.net or toll free at 866-607-3542 RELIA Solutions is the nation's ONLY recruiting firm specializing in the nationwide permanent placement of histology professionals. To sign up for our free histology careers bulletin please send an e-mail to relia1@earthlink.net and include subscribe in the subject line. Keywords: Histology, histologist, histotechnician, histotechnologist, anatomic pathology If you or anyone you know might be interested in more information about this position please contact Pam Barker at relia1@earthlink.net or toll free at 866-607-3542. Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From one_angel_secret <@t> yahoo.com Mon Mar 11 12:47:55 2013 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Mon Mar 11 12:48:10 2013 Subject: [Histonet] Fw: news In-Reply-To: <1363016329.73457.YahooMailNeo@web161602.mail.bf1.yahoo.com> References: <1363016329.73457.YahooMailNeo@web161602.mail.bf1.yahoo.com> Message-ID: Disregard something is up with my account. Sent from my iPhone On Mar 11, 2013, at 11:38 AM, Kim Donadio wrote: > http://www.britvoice.co.uk/wwvc/woszquloax/hkjmnqu=bvdzq > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From victor.leyva-grado <@t> mssm.edu Mon Mar 11 14:37:29 2013 From: victor.leyva-grado <@t> mssm.edu (Leyva-Grado, Victor) Date: Mon Mar 11 14:37:35 2013 Subject: [Histonet] Detecting monoclonas in mouse lung Message-ID: Dear all, I have a couple of monoclonal antibodies raised against a respiratory virus. I used as treatment for disease and they worked. In trying to elucidate some mechanisms, I'm planning to biotinylate the antibody and at specific time points collect the lungs and do IHC to determine the distribution of the monoclonal. Do you think this will be feasible? I'm also planning to use some section for double labeling IF to determine the site of interaction antigen-antibody. Do you guys have any reference for this? Thanks a lot, Victor Victor H Leyva-Grado DVM, PhD Postdoctoral Fellow Microbiology Department Global Health and Emerging Pathogens Institute Icahn School of Medicine at Mount Sinai One Gustave L Levy Place Box 1124 Annenberg 16-15 New York, NY 10029 Phone 1-212-241-7094 Fax 1-212-534-1684 From Vickroy.Jim <@t> mhsil.com Tue Mar 12 08:11:09 2013 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Tue Mar 12 08:11:19 2013 Subject: [Histonet] Reagent containers for VIP 3000 Message-ID: We have two old VIP 3000's and are trying to keep them running. Unfortunately finding some parts are getting g very hard. Can anyone steer me to who might have some old reagent containers for the VIP 3000? We have one container that started leaking yesterday and I suspect this is going to be an ongoing problem because of the age of the instrument. Thanks for your assistance. Jim James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From Vickroy.Jim <@t> mhsil.com Tue Mar 12 13:48:59 2013 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Tue Mar 12 13:49:05 2013 Subject: [Histonet] Placentas - Requests for Homeopathic remedy usage Message-ID: We are occasionally getting requests for a patient to take home a placenta. Our hospital policy does not require that placentas are sent to pathology unless requested by the clinician. The placentas that are being requested are ones that normally would not come to pathology for examination and are supposedly being used in homeopathic placental remedies. Currently our hospital policy does not allow for tissue to be given to the patient to take home. I am interested in how other hospitals are handling these requests. Thanks for your time. Jim James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From sdysart <@t> mirnarx.com Tue Mar 12 14:07:28 2013 From: sdysart <@t> mirnarx.com (Sarah Dysart) Date: Tue Mar 12 14:07:43 2013 Subject: [Histonet] Citadel Message-ID: <8A70A9B2ECDD084DACFE6C59FCF86D5055F0CE5D@BL2PRD0711MB434.namprd07.prod.outlook.com> Thank god this thing has finally DIED!!! I need to order used equipment from a Texas source (sorry Denise...I would love to order from you again, but I have to order from a Texas company...). Does anyone know of a good used equipment company in Texas that sells processors? Thanks Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 From jadepasquale <@t> morphogenyx.com Tue Mar 12 16:02:25 2013 From: jadepasquale <@t> morphogenyx.com (jadepasquale) Date: Tue Mar 12 15:02:39 2013 Subject: [Histonet] microscope camera Message-ID: <513F97E1.4040500@morphogenyx.com> Solomon, Try the TIS camera site: http://www.theimagingsource.com/en_US/ -- Joseph A DePasquale, PhD Morphogenyx Inc From trathborne <@t> somerset-healthcare.com Tue Mar 12 16:16:07 2013 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Tue Mar 12 16:17:15 2013 Subject: [Histonet] RE: Placentas - Requests for Homeopathic remedy usage In-Reply-To: References: Message-ID: <3AD061FE740D464FAC7BF6B5CFB757076A282EA6@smcmail02.somerset-healthcare.com> This was discussed at great length last year. You can check the archives for a lot of responses. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Tuesday, March 12, 2013 2:49 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Placentas - Requests for Homeopathic remedy usage We are occasionally getting requests for a patient to take home a placenta. Our hospital policy does not require that placentas are sent to pathology unless requested by the clinician. The placentas that are being requested are ones that normally would not come to pathology for examination and are supposedly being used in homeopathic placental remedies. Currently our hospital policy does not allow for tissue to be given to the patient to take home. I am interested in how other hospitals are handling these requests. Thanks for your time. Jim James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Tue Mar 12 18:56:00 2013 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Tue Mar 12 18:56:04 2013 Subject: [Histonet] New HT program needs helps Message-ID: Merrit College, in Oakland, California, is in the process of starting a new Histotechnology program. They are looking for members for their advisory board, faculty, support with donations, advice, etc. If you are interested in being part of this start-up program please contact either/both of the people below. If you have any questions you may also contact me. Thanks, Jennifer MacDonald Gisele Giorgi, ggiorgi@peralta.edu, Director, Merritt Microscopy Program, www.merrittmicroscopy.net Hank Fabian, hfabian@peralta.edu, Director, Merritt Genomics Program, 510-436-2618 From Marilyn.Tyler <@t> uct.ac.za Wed Mar 13 05:27:07 2013 From: Marilyn.Tyler <@t> uct.ac.za (Marilyn Tyler) Date: Wed Mar 13 05:28:09 2013 Subject: [Histonet] MUC3 Message-ID: <5140709B020000900018CDCD@gwiasmtp.uct.ac.za> Hi Histonetters Does anyone use MUC3(mucin) antibody that is working on FFPE human tissue? Many Thanks Marilyn Tyler Medical School UCT Dept of Surgery J50/26 or 30 Surgical research. OMB Groote Schuur Hospital Observatory 021-4066227 ### UNIVERSITY OF CAPE TOWN This e-mail is subject to the UCT ICT policies and e-mail disclaimer published on our website at http://www.uct.ac.za/about/policies/emaildisclaimer/ or obtainable from +27 21 650 9111. This e-mail is intended only for the person(s) to whom it is addressed. If the e-mail has reached you in error, please notify the author. If you are not the intended recipient of the e-mail you may not use, disclose, copy, redirect or print the content. If this e-mail is not related to the business of UCT it is sent by the sender in the sender's individual capacity. ### From giuli.zunino <@t> gmail.com Wed Mar 13 05:31:29 2013 From: giuli.zunino <@t> gmail.com (Giulia Zunino) Date: Wed Mar 13 05:31:36 2013 Subject: [Histonet] Fixation Message-ID: I would like to have some informations about fetal brain fixation.. In general, it is in use the fomalin, but during the cut it is possible to see that in the deep layer the formalin doesn't arrive... So, Could you give some suggestion to improve this technique?! Thanks in advance Best Giulia Zunino, PhD Student Laboratory of Molecular Neuropathology Centre for Integrative Biology (CIBIO), University of Trento From mstone <@t> cmhlink.org Wed Mar 13 06:14:06 2013 From: mstone <@t> cmhlink.org (MARCELLYN A. STONE) Date: Wed Mar 13 06:14:11 2013 Subject: [Histonet] Embedding centers Message-ID: <6FF1D287A4C72844B37CF1B0FBAAB05C2FF7071B@exch-mbx-01.corp.cmhlink.org> Good morning, I have a tissue tek embedding center. The hot plate no longer works. I have been told I can not get it replaced until July and it is too expensive to repair. I have looked into barrowing or getting a loaner from someone who has a back-up. That has not worked. Anyone have any ideas on maybe a fix? Thanks Marcy CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for use by the designated recipients named above. They are intended solely for these recipients. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify Calvert Memorial Hospital immediately by telephone at (410)535-8282 and destroy all copies of this communication and any attachments. From rjbuesa <@t> yahoo.com Wed Mar 13 07:30:09 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Mar 13 07:30:16 2013 Subject: [Histonet] Fixation In-Reply-To: References: Message-ID: <1363177809.4292.YahooMailNeo@web163103.mail.bf1.yahoo.com> The fixation is not complete because fixation time is too short. Please go to http://www.histosearch.com/rene.html where you can find?two articles about formalin fixation where this issue is discussed. Ren? J. From: Giulia Zunino To: histonet@lists.utsouthwestern.edu Sent: Wednesday, March 13, 2013 6:31 AM Subject: [Histonet] Fixation I would like to have some informations about fetal brain fixation.. In general, it is in use the fomalin, but during the cut it is possible to see that in the deep layer the formalin doesn't arrive... So, Could you give some suggestion to improve this technique?! Thanks in advance Best Giulia Zunino, PhD Student Laboratory of Molecular Neuropathology Centre for Integrative Biology (CIBIO), University of Trento _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From trathborne <@t> somerset-healthcare.com Wed Mar 13 07:56:15 2013 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Wed Mar 13 07:56:11 2013 Subject: [Histonet] RE: Embedding centers In-Reply-To: <6FF1D287A4C72844B37CF1B0FBAAB05C2FF7071B@exch-mbx-01.corp.cmhlink.org> References: <6FF1D287A4C72844B37CF1B0FBAAB05C2FF7071B@exch-mbx-01.corp.cmhlink.org> Message-ID: <3AD061FE740D464FAC7BF6B5CFB757076A282FEA@smcmail02.somerset-healthcare.com> Maybe one of the used equipment vendors would consider a short-term lease with option to buy. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of MARCELLYN A. STONE Sent: Wednesday, March 13, 2013 7:14 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Embedding centers Good morning, I have a tissue tek embedding center. The hot plate no longer works. I have been told I can not get it replaced until July and it is too expensive to repair. I have looked into barrowing or getting a loaner from someone who has a back-up. That has not worked. Anyone have any ideas on maybe a fix? Thanks Marcy CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for use by the designated recipients named above. They are intended solely for these recipients. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify Calvert Memorial Hospital immediately by telephone at (410)535-8282 and destroy all copies of this communication and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Nancy_Schmitt <@t> pa-ucl.com Wed Mar 13 08:52:15 2013 From: Nancy_Schmitt <@t> pa-ucl.com (Nancy Schmitt) Date: Wed Mar 13 08:52:23 2013 Subject: [Histonet] CD68 Message-ID: <906B4DA90ED1DB4DB6C7E94D7CEE6C364DC730FC@PEITHA.wad.pa-ucl.com> Good Morning! CD68 (for the BOND)is backordered until May and I am looking for recommendations - Cellmarque or Biocare? Is there something better? I appreciate your input. Nancy Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From mcauliff <@t> umdnj.edu Wed Mar 13 09:06:13 2013 From: mcauliff <@t> umdnj.edu (Geoff) Date: Wed Mar 13 09:06:27 2013 Subject: [Histonet] Fixation In-Reply-To: References: Message-ID: <514087D5.2070606@umdnj.edu> Rene is correct, formalin fixes slowly so more time is needed. Raising the concentration of formaldehyde will not help. Adding a small amount of glutaraldehyde (0.25 to 0.5%) will fix more quickly but may have a negative effect on immunostaining. Geoff On 3/13/2013 6:31 AM, Giulia Zunino wrote: > I would like to have some informations about fetal brain fixation.. > In general, it is in use the fomalin, but during the cut it is possible to > see that in the deep layer the formalin doesn't arrive... > So, Could you give some suggestion to improve this technique?! > > Thanks in advance > > Best > > > > > > Giulia Zunino, PhD Student > > Laboratory of Molecular Neuropathology > Centre for Integrative Biology (CIBIO), University of Trento > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From SHUNTER <@t> beaumont.edu Wed Mar 13 10:58:02 2013 From: SHUNTER <@t> beaumont.edu (Sue Hunter) Date: Wed Mar 13 10:58:14 2013 Subject: [Histonet] RE: CD68 In-Reply-To: <906B4DA90ED1DB4DB6C7E94D7CEE6C364DC730FC@PEITHA.wad.pa-ucl.com> References: <906B4DA90ED1DB4DB6C7E94D7CEE6C364DC730FC@PEITHA.wad.pa-ucl.com> Message-ID: We use Ventana's ready to use for the Ultra, but it is clone KP1. You can get the same clone in a concentrate from Cell Marque. Sue Sue Hunter, Supervisor Advanced Diagnostics Beaumont Health System Royal Oak MI 248-898-5146 shunter@beaumont.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt Sent: Wednesday, March 13, 2013 9:52 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CD68 Good Morning! CD68 (for the BOND)is backordered until May and I am looking for recommendations - Cellmarque or Biocare? Is there something better? I appreciate your input. Nancy Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pbourassa <@t> karospharma.com Wed Mar 13 10:58:27 2013 From: pbourassa <@t> karospharma.com (Patricia Bourassa) Date: Wed Mar 13 10:58:36 2013 Subject: [Histonet] SMC Immuno and Elastin combo? Message-ID: Hello, I've read some papers which reference the combination of smooth muscle cell actin immunostaining with a Verhoeff's elastin counterstain. Has anyone ever done this and if so, do you have protocol you'd be willing to share? I'm staining mouse and rat lung and have a SMC protocol working that uses an Abcam antibody and NovaRed chromagen (peroxidase). I've also done EVG staining so I'm familiar with the general process. The combination is what I'm not sure of - I gave it a try for the heck of it and wiped out the immunostain. We've just started looking at this, so nothing is set in stone and I'd be grateful for any advice/ procedural help. Thanks in advance! Patti Bourassa Karos Pharmaceuticals -- *Patti Bourassa Senior Scientist Karos Pharmaceuticals (203) 535-0540, ext 207* From nto <@t> stowers.org Wed Mar 13 11:32:54 2013 From: nto <@t> stowers.org (Thomas, Nancy) Date: Wed Mar 13 11:33:29 2013 Subject: [Histonet] SMC Immuno and Elastin combo? In-Reply-To: References: Message-ID: Patti, The immunostaining probably was not wiped out, rather just covered up with the red color of the VG counterstain. You might try a chromogen that expresses in a different color. Or, you might leave off the van gieson part of the EVG (staining only the elastic tissue) and pick a counterstain of another color. Basically, you just need to make sure that none of the stains used have the same color outcome. I have done this combo in the past. Never really finished the project because the issue I had was that the lizard tissue I was working with just would not stay on the slide no matter what I tried. But, I do remember that I treated them as two separate stains. I did the actin staining first, rinsed it well, then started in on the elastin stain. You probably have a protocol for each of these and you just need to combine them. Nancy Thomas Stowers Institute -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patricia Bourassa Sent: Wednesday, March 13, 2013 10:58 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] SMC Immuno and Elastin combo? Hello, I've read some papers which reference the combination of smooth muscle cell actin immunostaining with a Verhoeff's elastin counterstain. Has anyone ever done this and if so, do you have protocol you'd be willing to share? I'm staining mouse and rat lung and have a SMC protocol working that uses an Abcam antibody and NovaRed chromagen (peroxidase). I've also done EVG staining so I'm familiar with the general process. The combination is what I'm not sure of - I gave it a try for the heck of it and wiped out the immunostain. We've just started looking at this, so nothing is set in stone and I'd be grateful for any advice/ procedural help. Thanks in advance! Patti Bourassa Karos Pharmaceuticals -- *Patti Bourassa Senior Scientist Karos Pharmaceuticals (203) 535-0540, ext 207* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From shive003 <@t> umn.edu Wed Mar 13 11:45:03 2013 From: shive003 <@t> umn.edu (Jan Shivers) Date: Wed Mar 13 11:45:08 2013 Subject: [Histonet] SMC Immuno and Elastin combo? In-Reply-To: References: Message-ID: Patti, You're using a mouse monoclonal antibody on mouse tissue, so be aware that the secondary link antibody will bind to any mouse IgG it finds in the tissue (normal plasma cells, IgG in normal sera present inside blood vessels, or as infiltrating interstitial normal sera, etc.), not just to the primary anti-Actin antibody. You may have to use a mouse-on-mouse kit for the Actin staining portion, if you see too much nonspecific background in your tissue. Jan Shivers UMN Vet Diag Lab On Wed, Mar 13, 2013 at 10:58 AM, Patricia Bourassa < pbourassa@karospharma.com> wrote: > Hello, > > I've read some papers which reference the combination of smooth muscle cell > actin immunostaining with a Verhoeff's elastin counterstain. > > Has anyone ever done this and if so, do you have protocol you'd be willing > to share? > > I'm staining mouse and rat lung and have a SMC protocol working that uses > an Abcam antibody and NovaRed chromagen (peroxidase). I've also done EVG > staining so I'm familiar with the general process. The combination is what > I'm not sure of - I gave it a try for the heck of it and wiped out the > immunostain. > > We've just started looking at this, so nothing is set in stone and I'd be > grateful for any advice/ procedural help. > > Thanks in advance! > > Patti Bourassa > Karos Pharmaceuticals > -- > *Patti Bourassa > Senior Scientist > Karos Pharmaceuticals > (203) 535-0540, ext 207* > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From l.kammili <@t> yahoo.com Wed Mar 13 13:58:11 2013 From: l.kammili <@t> yahoo.com (Lakshmi Kammili) Date: Wed Mar 13 13:58:15 2013 Subject: [Histonet] Issues with Shandon Pathcentre Message-ID: <1363201091.54711.YahooMailNeo@web121003.mail.ne1.yahoo.com> Hi all, ?I have some issues with Shandon Pathcentre. Though the machine is kind of old it was very perfect in shape and running good. ?I am posting this request to see if any of other labs faced this issue before and if any one can give me suggestions. It was running very fine and perfect, until it showed an error recently saying "temperature too high on rotary chamber". I checked the settings and it was on 35 which I assume is fine. All my waxes are also at fine temperature. I changed all the waxes and reagents to see if its a false sensor. But did not help. I changed the?protocol?also from 35 to ambient temperature, did not work. I reset the?memory?completely , did not work. I tried calling some repair places like Dolbey-Jamison and few other places. Most of them say they dont work with this machine any more.? I would appreciate if any one can suggest me any advice, regarding handling the issue differently or if any one know a lab repair service who deals with this kind old machines is much appreciated. Thank you, Lakshmi Kammili, Sr. Research Associate, School of medicine and health sciences, George Washington University, Ross hall, #725 2300 eye St, NW Washington DC. From rsrichmond <@t> gmail.com Wed Mar 13 14:35:25 2013 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Wed Mar 13 14:35:33 2013 Subject: [Histonet] Re: Placenta - Requests for Homeopathic remedy usage Message-ID: We indeed discussed "placenta encapsulation" on Histonet about a year ago. http://lists.utsouthwestern.edu/pipermail/histonet/2012-March/061671.html There are also placental homeopathic remedies: http://lists.utsouthwestern.edu/pipermail/histonet/2012-March/061671.html though I'm not sure that soaking a placenta in brandy for a week is exactly what Dr. Hahnemann prescribed. Bob Richmond Samurai Pathologist Maryville TN From stighe <@t> ufl.edu Wed Mar 13 15:48:07 2013 From: stighe <@t> ufl.edu (Tighe,Sean T) Date: Wed Mar 13 15:48:10 2013 Subject: [Histonet] Alizarin Red S Staining Protocol Message-ID: <14851f3cd0e28ac91b3b1ab24e6a84cd@ufl.edu> Good afternoon, In the past I have been using a 40mM Alizarin Red S Solution from Millipore to stain my cell cultures but I am now seeking an alternative. I plan on preparing fresh Alizarin Red S by adding the powder to 100ml of distilled water. In reviewing the literature, I have come across many labs using 1% ARS or 2% ARS and I am not sure if this concentration significantly matters. Furthermore, I understand some people use McGee-Russell's procedure using a pH of ~4.2 whereas others use Dahl's procedure using a pH of ~6.3. However using a pH of 4.2 seems illogical to me as this could remove some calcium from the monolayer. Currently I plan on fixing the cells with 4% Paraformaldehyde in PBS (pH 7) for 15 minutes, washing twice with water and then staining the cells with 2% ARS in water (pH 6.3). Do you see any problems with this? Also, should I stain for 5 minutes or roughly an hour with the ARS dye? I have had some problems with extracting this dye in the past. The 10% acetic acid does not seem to remove all of the dye and the results are variable. Note: before I extract the cells with acetic acid I wash the cells four times with water. Regards, Sean Tighe From ratliffjack <@t> hotmail.com Wed Mar 13 17:05:13 2013 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Wed Mar 13 17:05:23 2013 Subject: [Histonet] All Members Working with Bone, Biomaterials and Medical Device Implants Message-ID: On May the 4th, 2013, Polysciences, Inc. is hosting a full day educational event focusing on the Histological Applications & Techniques for Bone, Biomaterials and Medical Device Implants. On behalf of the speakers (Bob Skinner, Philip Seifert, Valantou Grover and Jack Ratliff) contributing to the educational content for this event, we would like for you to join us in Cambridge, MA at the Hyatt Regency Cambridge (overlooking Boston). With our combined histology experience of 90+ years in working with clinical and preclinical specimens pertaining to bone, biomaterials and medical device implants, you can expect to further your knowledge, understanding and training of specialized histology techniques associated with these specimen types and the applicational relevance of these techniques used in the evaluation safety and efficacy of therapeutic treatments. Registration for this event is now open with Early Bird registration set to end one week from Friday on March 22nd. The National Society for Histotechnology (NSH) will be providing 6 CEUs and Polysciences, Inc. will be providing a discount to any current NSH member as outlined below: WORKSHOP FEES Before March 22, 2013: $149.00 for NSH Members / $199.00 for Non-Members After March 22, 2013: $179.00 for NSH Members / $229.00 for Non-Members For the complete details of this full day Histological Applications & Techniques for Bone, Biomaterials and Medical Device Implants event and to register online, please visit the following link: http://www.polysciences.com/Interactive-Histology-Forum-About/185/ and sign up today to participate in the discussion of these specialized histology specimen applications and techniques that are rarely shared or even discussed on Histonet! You will not want to miss out on the information presented by 4 expert speakers in the field, all course materials, meals and a complete program book also containing technique specific protocols that you can repeat back in your lab! We hope to see you in Cambridge (Boston) and May the 4th be with you! Best Regards,Jack Ratliff From tony.henwood <@t> health.nsw.gov.au Wed Mar 13 18:13:09 2013 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Wed Mar 13 18:13:28 2013 Subject: [Histonet] SMC Immuno and Elastin combo? In-Reply-To: References: Message-ID: <6D6BD1DE8A5571489398B392A38A71579D236E4F@xmdb04.nch.kids> Nancy, The red of the classic VG counterstain stains the collagen. Muscle will appear yellow. If the yellow is obscuring the brown DAB then after VG staining wash the slides in water. This will remove the picric acid staining leaving the muscle brown. Since Nova red is red (I assume since I have not had cause to use it - I'm a DAB man!), I would use Picro Indigo carmine or Picro-Light green. Picro-indigo Solution (Ortiz-Hidalgo C "Abelardo Gallego(1879-1930)and his contributions to histotechnology: The Gallego stains" Acta histochemica1 13(2011):189-193): Picric acid (saturated solution) 100ml Indigo carmine 0.25mg Distilled water 15ml Whippy's Elastic Stain (Whippy P "A Stain to Try - Whippy's Elastic Stain" Histograph Jan 2008:7): Light Green Stock Solution Light Green 2 gm Distilled water 100 ml Glacial Acetic Acid 2 ml Place Light Green and distilled water into a conical flask and mix well. Add Acetic acid and mix. Filter and pour into stock bottle. Light Green - Picrate (Whippy's) Solution Saturated aqueous picric acid 10 ml Light Green stock solution 5 drops Pour Picric Acid into a conical flask. Add Light Green solution, stir until dissolved. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Thomas, Nancy Sent: Thursday, 14 March 2013 3:33 AM To: 'Patricia Bourassa'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] SMC Immuno and Elastin combo? Patti, The immunostaining probably was not wiped out, rather just covered up with the red color of the VG counterstain. You might try a chromogen that expresses in a different color. Or, you might leave off the van gieson part of the EVG (staining only the elastic tissue) and pick a counterstain of another color. Basically, you just need to make sure that none of the stains used have the same color outcome. I have done this combo in the past. Never really finished the project because the issue I had was that the lizard tissue I was working with just would not stay on the slide no matter what I tried. But, I do remember that I treated them as two separate stains. I did the actin staining first, rinsed it well, then started in on the elastin stain. You probably have a protocol for each of these and you just need to combine them. Nancy Thomas Stowers Institute -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patricia Bourassa Sent: Wednesday, March 13, 2013 10:58 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] SMC Immuno and Elastin combo? Hello, I've read some papers which reference the combination of smooth muscle cell actin immunostaining with a Verhoeff's elastin counterstain. Has anyone ever done this and if so, do you have protocol you'd be willing to share? I'm staining mouse and rat lung and have a SMC protocol working that uses an Abcam antibody and NovaRed chromagen (peroxidase). I've also done EVG staining so I'm familiar with the general process. The combination is what I'm not sure of - I gave it a try for the heck of it and wiped out the immunostain. We've just started looking at this, so nothing is set in stone and I'd be grateful for any advice/ procedural help. Thanks in advance! Patti Bourassa Karos Pharmaceuticals -- *Patti Bourassa Senior Scientist Karos Pharmaceuticals (203) 535-0540, ext 207* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From erinandjesseconley <@t> yahoo.com Wed Mar 13 19:34:00 2013 From: erinandjesseconley <@t> yahoo.com (Erin and Jesse Conley) Date: Wed Mar 13 19:34:03 2013 Subject: [Histonet] hot copy Message-ID: <1363221240.60371.YahooMailNeo@web141003.mail.bf1.yahoo.com> http://www.petfriendly.es/llahg/hxlke.wcdarfmxxhbo From lpwenk <@t> sbcglobal.net Wed Mar 13 21:20:32 2013 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Wed Mar 13 21:20:33 2013 Subject: [Histonet] Alizarin Red S Staining Protocol In-Reply-To: <14851f3cd0e28ac91b3b1ab24e6a84cd@ufl.edu> References: <14851f3cd0e28ac91b3b1ab24e6a84cd@ufl.edu> Message-ID: <1C99BBDAD19B41CE9C8FCC5CD05A897E@HP2010> We always used a 2% Alizarin red S (never tried it with 1%. I'm guessing that you would just have to leave it in longer.) We pH it to 4.1-4.3 with 0.5% ammonium hydroxide. I don't remember what the pH is when we begin, but note, we're adding a BASE to bring the pH up to ~4.2. So alizarin red S must be fairly acidic when dissolved in water. I was told that this is a chelating solution for soft metals. It therefore is not a specific stain for calcium, as it will also stain magnesium, manganese, barium, and strontium. However, these metals are usually not in very high concentrations. But to make it more specific for calcium, a pH of 4.1-4.3 is recommended. I've never done this stain on cell cultures, only on formalin fixed, paraffin embedded, 5 um thick sections. And we find that staining between 1-2 minutes works the best. This is a very sensitive stain. The longer the slide is left in the solution, the more and more the alizarin red S stains the background, to the point that it becomes difficult to tell positive calcium from background staining. Every cell has calcium in it (think membrane transport), and eventually, all the cells are going to pick up background orange color, not just the lesions with calcium. You mention extraction with acetic acid. Extracting what? Are you trying to extract the background staining? I don't know if that will work. This is a chelating agent . . . the CLAW! Once chelating agents hook up with the metal, they don't let go. I just don't happen to know if acid will disrupt this chelating bond. So it would be better to cut the time way down (try 1-2 minutes, see how it looks), and don't have so much background staining to begin with. Let us know how it turns out. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Health System Royal Oak, MI 48073 Opinions expressed are mine, and do not reflect upon my place of employment. -----Original Message----- From: Tighe,Sean T Sent: Wednesday, March 13, 2013 4:48 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Alizarin Red S Staining Protocol Good afternoon, In the past I have been using a 40mM Alizarin Red S Solution from Millipore to stain my cell cultures but I am now seeking an alternative. I plan on preparing fresh Alizarin Red S by adding the powder to 100ml of distilled water. In reviewing the literature, I have come across many labs using 1% ARS or 2% ARS and I am not sure if this concentration significantly matters. Furthermore, I understand some people use McGee-Russell's procedure using a pH of ~4.2 whereas others use Dahl's procedure using a pH of ~6.3. However using a pH of 4.2 seems illogical to me as this could remove some calcium from the monolayer. Currently I plan on fixing the cells with 4% Paraformaldehyde in PBS (pH 7) for 15 minutes, washing twice with water and then staining the cells with 2% ARS in water (pH 6.3). Do you see any problems with this? Also, should I stain for 5 minutes or roughly an hour with the ARS dye? I have had some problems with extracting this dye in the past. The 10% acetic acid does not seem to remove all of the dye and the results are variable. Note: before I extract the cells with acetic acid I wash the cells four times with water. Regards, Sean Tighe _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From max_histo_00 <@t> yahoo.it Thu Mar 14 02:28:28 2013 From: max_histo_00 <@t> yahoo.it (Massimo) Date: Thu Mar 14 02:31:42 2013 Subject: [Histonet] Fixation In-Reply-To: References: Message-ID: <1363246108.91354.YahooMailNeo@web172006.mail.ir2.yahoo.com> Hi, you can find a pratical suggestion at your problem by watching the Step 9: "Expedite Large Specimen Fixation" on the guide: "101 Steps to Better Histology - a Practical Guide to Good Histology Practice" You can download it from: http://www.leicabiosystems.com/pathologyleaders/101-steps-to-better-histology-a-practical-guide-to-good-histology-practice/ I don't know if such procedure is suitable for the study of your specimen but take it just like a suggestion. My Best. Massimo Tosi "A humble Chemical Engineer who loves Histology" ________________________________ Da: Giulia Zunino A: histonet@lists.utsouthwestern.edu Inviato: Mercoled? 13 Marzo 2013 11:31 Oggetto: [Histonet] Fixation I would like to have some informations about fetal brain fixation.. In general, it is in use the fomalin, but during the cut it is possible to see that in the deep layer the formalin doesn't arrive... So, Could you give some suggestion to improve this technique?! Thanks in advance Best Giulia Zunino, PhD Student Laboratory of Molecular Neuropathology Centre for Integrative Biology (CIBIO), University of Trento _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ibernard <@t> uab.edu Thu Mar 14 07:42:30 2013 From: ibernard <@t> uab.edu (Ian R Bernard) Date: Thu Mar 14 07:42:41 2013 Subject: [Histonet] AFB and Negative Control In-Reply-To: <1C99BBDAD19B41CE9C8FCC5CD05A897E@HP2010> References: <14851f3cd0e28ac91b3b1ab24e6a84cd@ufl.edu> <1C99BBDAD19B41CE9C8FCC5CD05A897E@HP2010> Message-ID: The only special stain that I know that requires the use of a negative control is for the AFB. I understand to rule out false positives as the AFB bacteria might exist in tap water. Nevertheless, a good QA practice which we will implement now. Other than Carson, does anyone know of a regulatory or accreditation agency is requiring this as well? Any suggestion on a good control tissue type? Carson recommends uterus. Also if there is a pick up on the negative slide (link to the tap water) will use of distilled water and a repeat procedure fix this? Any thoughts from fellow histonetters? Thanks Ian Bernard -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk Sent: Wednesday, March 13, 2013 9:21 PM To: Tighe,Sean T; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Alizarin Red S Staining Protocol We always used a 2% Alizarin red S (never tried it with 1%. I'm guessing that you would just have to leave it in longer.) We pH it to 4.1-4.3 with 0.5% ammonium hydroxide. I don't remember what the pH is when we begin, but note, we're adding a BASE to bring the pH up to ~4.2. So alizarin red S must be fairly acidic when dissolved in water. I was told that this is a chelating solution for soft metals. It therefore is not a specific stain for calcium, as it will also stain magnesium, manganese, barium, and strontium. However, these metals are usually not in very high concentrations. But to make it more specific for calcium, a pH of 4.1-4.3 is recommended. I've never done this stain on cell cultures, only on formalin fixed, paraffin embedded, 5 um thick sections. And we find that staining between 1-2 minutes works the best. This is a very sensitive stain. The longer the slide is left in the solution, the more and more the alizarin red S stains the background, to the point that it becomes difficult to tell positive calcium from background staining. Every cell has calcium in it (think membrane transport), and eventually, all the cells are going to pick up background orange color, not just the lesions with calcium. You mention extraction with acetic acid. Extracting what? Are you trying to extract the background staining? I don't know if that will work. This is a chelating agent . . . the CLAW! Once chelating agents hook up with the metal, they don't let go. I just don't happen to know if acid will disrupt this chelating bond. So it would be better to cut the time way down (try 1-2 minutes, see how it looks), and don't have so much background staining to begin with. Let us know how it turns out. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Health System Royal Oak, MI 48073 Opinions expressed are mine, and do not reflect upon my place of employment. -----Original Message----- From: Tighe,Sean T Sent: Wednesday, March 13, 2013 4:48 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Alizarin Red S Staining Protocol Good afternoon, In the past I have been using a 40mM Alizarin Red S Solution from Millipore to stain my cell cultures but I am now seeking an alternative. I plan on preparing fresh Alizarin Red S by adding the powder to 100ml of distilled water. In reviewing the literature, I have come across many labs using 1% ARS or 2% ARS and I am not sure if this concentration significantly matters. Furthermore, I understand some people use McGee-Russell's procedure using a pH of ~4.2 whereas others use Dahl's procedure using a pH of ~6.3. However using a pH of 4.2 seems illogical to me as this could remove some calcium from the monolayer. Currently I plan on fixing the cells with 4% Paraformaldehyde in PBS (pH 7) for 15 minutes, washing twice with water and then staining the cells with 2% ARS in water (pH 6.3). Do you see any problems with this? Also, should I stain for 5 minutes or roughly an hour with the ARS dye? I have had some problems with extracting this dye in the past. The 10% acetic acid does not seem to remove all of the dye and the results are variable. Note: before I extract the cells with acetic acid I wash the cells four times with water. Regards, Sean Tighe _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TNMayer <@t> mdanderson.org Thu Mar 14 12:52:39 2013 From: TNMayer <@t> mdanderson.org (Mayer,Toysha N) Date: Thu Mar 14 12:52:42 2013 Subject: [Histonet] RE: AFB and negative control Message-ID: <47E9B2C01DDDD94881EACD2DC44EBC880157985C@D1PWPEXMBX05.mdanderson.edu> While I don't use a negative control for the AFB, I will use distilled water throughout the procedure. Most of the time the water in the waterbath is distilled as well, to rule out contamination there as well. Make sure the waterbath has been disinfected. Toysha N. Mayer, MBA, HT (ASCP) Instructor, Education Coordinator Program in Histotechnology School of Health Professions MD Anderson Cancer Center (713) 563-3481 tnmayer@mdanderson.org Date: Thu, 14 Mar 2013 12:42:30 +0000 From: Ian R Bernard Subject: [Histonet] AFB and Negative Control To: Lee & Peggy Wenk , "Tighe, Sean T" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="utf-8" The only special stain that I know that requires the use of a negative control is for the AFB. I understand to rule out false positives as the AFB bacteria might exist in tap water. Nevertheless, a good QA practice which we will implement now. Other than Carson, does anyone know of a regulatory or accreditation agency is requiring this as well? Any suggestion on a good control tissue type? Carson recommends uterus. Also if there is a pick up on the negative slide (link to the tap water) will use of distilled water and a repeat procedure fix this? Any thoughts from fellow histonetters? Thanks Ian Bernard From BZIMMERM <@t> gru.edu Thu Mar 14 13:39:27 2013 From: BZIMMERM <@t> gru.edu (Zimmerman, Billie) Date: Thu Mar 14 13:41:04 2013 Subject: [Histonet] SOS EGFRV3 Message-ID: <7B3DEB32E69C034EACB479059C5DE3FF75B795@EX-MLB-03.ad.georgiahealth.edu> Does anyone perform this particular clone of EGFR?? Thanks in advance for your help. Billie Zimmerman MT(ASCP)QIHC 706-721-5617/3630 Augusta State University and Georgia Health Sciences University have consolidated to become Georgia Regents University. Effective January 9, 2013, my email address has changed to BZIMMERM@gru.edu. Please update your address book to reflect this change. From brannon <@t> alliedsearchpartners.com Thu Mar 14 14:14:22 2013 From: brannon <@t> alliedsearchpartners.com (Brannon Owens) Date: Thu Mar 14 14:15:03 2013 Subject: [Histonet] Technical Laboratory Coordinator of Pathology Surgical Services- Atlanta, GA Message-ID: Full time/permanent opening for a Lab Coordinator of Pathology Surgical Services. Ideal candidate is HT/HTL/CT certified with at least 4 years of supervisory experience. Send an email to Brannon@alliedsearchpartners.com for a full job description. To view a complete list of Allied Search Partners current openings go to: http://www.alliedsearchpartners.com/careers.php -- Brannon Owens Recruitment Manager Allied Search Partners From Marilyn.A.Weiss <@t> kp.org Thu Mar 14 14:31:50 2013 From: Marilyn.A.Weiss <@t> kp.org (Marilyn.A.Weiss@kp.org) Date: Thu Mar 14 14:32:19 2013 Subject: [Histonet] Re: Histonet Digest, Vol 112, Issue 13 In-Reply-To: <201303121703.r2CH38Dg002213@imp6.kp.org> References: <201303121703.r2CH38Dg002213@imp6.kp.org> Message-ID: Have a question to ask the group. One of the Pathologists is complaining about anucleate squames on her breast biopsies. Blames the tech for fingers being in the water bath, which may or may not happen. If she recuts the case it is great and she does not do anything different. what could cause this phenomenon? molds? It is driving us crazy. HELP NOTICE TO RECIPIENT: If you are not the intended recipient of this e-mail, you are prohibited from sharing, copying, or otherwise using or disclosing its contents. If you have received this e-mail in error, please notify the sender immediately by reply e-mail and permanently delete this e-mail and any attachments without reading, forwarding or saving them. Thank you. From: histonet-request@lists.utsouthwestern.edu To: histonet@lists.utsouthwestern.edu Date: 03/12/2013 10:03 AM Subject: Histonet Digest, Vol 112, Issue 13 Sent by: histonet-bounces@lists.utsouthwestern.edu Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Fw: news (Kim Donadio) 2. Detecting monoclonas in mouse lung (Leyva-Grado, Victor) 3. Reagent containers for VIP 3000 (Vickroy, Jim) ---------------------------------------------------------------------- Message: 1 Date: Mon, 11 Mar 2013 13:47:55 -0400 From: Kim Donadio Subject: Re: [Histonet] Fw: news To: Kim Donadio Cc: histonet , confirm unsub 2m2s2bpqnls4gcuu00bqawlkaga4rn0q , Pathrm35 , PensacolaToyBreedAdoption unsubscribe Message-ID: Content-Type: text/plain; charset=us-ascii Disregard something is up with my account. Sent from my iPhone On Mar 11, 2013, at 11:38 AM, Kim Donadio wrote: > http://www.britvoice.co.uk/wwvc/woszquloax/hkjmnqu=bvdzq > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Mon, 11 Mar 2013 19:37:29 +0000 From: "Leyva-Grado, Victor" Subject: [Histonet] Detecting monoclonas in mouse lung To: "'histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain; charset="us-ascii" Dear all, I have a couple of monoclonal antibodies raised against a respiratory virus. I used as treatment for disease and they worked. In trying to elucidate some mechanisms, I'm planning to biotinylate the antibody and at specific time points collect the lungs and do IHC to determine the distribution of the monoclonal. Do you think this will be feasible? I'm also planning to use some section for double labeling IF to determine the site of interaction antigen-antibody. Do you guys have any reference for this? Thanks a lot, Victor Victor H Leyva-Grado DVM, PhD Postdoctoral Fellow Microbiology Department Global Health and Emerging Pathogens Institute Icahn School of Medicine at Mount Sinai One Gustave L Levy Place Box 1124 Annenberg 16-15 New York, NY 10029 Phone 1-212-241-7094 Fax 1-212-534-1684 ------------------------------ Message: 3 Date: Tue, 12 Mar 2013 08:11:09 -0500 From: "Vickroy, Jim" Subject: [Histonet] Reagent containers for VIP 3000 To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" We have two old VIP 3000's and are trying to keep them running. Unfortunately finding some parts are getting g very hard. Can anyone steer me to who might have some old reagent containers for the VIP 3000? We have one container that started leaking yesterday and I suspect this is going to be an ongoing problem because of the age of the instrument. Thanks for your assistance. Jim James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 112, Issue 13 ***************************************** From maria.navas <@t> utah.edu Thu Mar 14 15:04:43 2013 From: maria.navas <@t> utah.edu (Maria Navas-Moreno) Date: Thu Mar 14 15:06:31 2013 Subject: [Histonet] Thawing brain tissue Message-ID: Hello Histonet community, I recently froze a primate brain using isopentane immerse in a bath of 100% ethanol and chunks of dry ice. Two out of the 3 blocks froze fine but the third one shows a very bad convex surface, although to be fair, the defective block was embedded in gelatin while the other ones weren't. We have had this issue before, even without the embedding, and my PI thinks it could be due in part to a bad blocking procedure in which the surface is not completely flat. Before we were able to "deal" with the convex defect. This time it is so bad that we think it should be better to thaw and re-freeze. When dealing with thawing is usually for other purposes (i.e., live cells retrieval) and I think it is suggested to thaw very fast, to avoid water crystal issues. But in the case of morphology and IHC, will it be beneficial to thaw quickly? I am trying to learn from others experience so I can try so salvage this sample so any information is appreciated. I know this is far from being a standard procedure but since the block got so deformed I don't think there is any other option, I am just trying to find the optimum conditions for a non-ideal situation. Thanks Maria Navas From maria.navas <@t> utah.edu Thu Mar 14 15:29:39 2013 From: maria.navas <@t> utah.edu (Maria De Los Angeles Navas) Date: Thu Mar 14 15:28:48 2013 Subject: [Histonet] Thawing brain tissue Message-ID: Hello Histonet community, I recently froze a primate brain using isopentane immerse in a bath of 100% ethanol and chunks of dry ice. Two out of the 3 blocks froze fine but the third one shows a very bad convex surface, although to be fair, the defective block was embedded in gelatin while the other ones weren't. We have had this issue before, even without the embedding, and my PI thinks it could be due in part to a bad blocking procedure in which the surface is not completely flat. Before we were able to "deal" with the convex defect. This time it is so bad that we think it should be better to thaw and re-freeze. When dealing with thawing is usually for other purposes (i.e., live cells retrieval) and I think it is suggested to thaw very fast, to avoid water crystal issues. But in the case of morphology and IHC, will it be beneficial to thaw quickly? I am trying to learn from others experience so I can try so salvage this sample so any information is appreciated. I know this is far from being a standard procedure but since the block got so deformed I don't think there is any other option, I am just trying to find the optimum conditions for a non-ideal situation. Thanks, Maria From joelleweaver <@t> hotmail.com Thu Mar 14 15:51:25 2013 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Thu Mar 14 15:51:32 2013 Subject: [Histonet] RE: AFB and negative control In-Reply-To: <47E9B2C01DDDD94881EACD2DC44EBC880157985C@D1PWPEXMBX05.mdanderson.edu> References: <47E9B2C01DDDD94881EACD2DC44EBC880157985C@D1PWPEXMBX05.mdanderson.edu> Message-ID: This practice is listed as a QC measure for issues of cross contamination in the ASCP publication "Quality Management in Anatomic Pathology, Nakhleh, R. M.D. I have never had any issues that were persistant enough to warrant this measure myself, but it is one of the suggestions made under the section for use of control tissue/slides. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: TNMayer@mdanderson.org > To: histonet@lists.utsouthwestern.edu > Date: Thu, 14 Mar 2013 17:52:39 +0000 > Subject: [Histonet] RE: AFB and negative control > > While I don't use a negative control for the AFB, I will use distilled water throughout the procedure. Most of the time the water in the waterbath is distilled as well, to rule out contamination there as well. Make sure the waterbath has been disinfected. > > Toysha N. Mayer, MBA, HT (ASCP) > Instructor, Education Coordinator > Program in Histotechnology > School of Health Professions > MD Anderson Cancer Center > (713) 563-3481 > tnmayer@mdanderson.org > > > > > Date: Thu, 14 Mar 2013 12:42:30 +0000 > From: Ian R Bernard > Subject: [Histonet] AFB and Negative Control > To: Lee & Peggy Wenk , "Tighe, Sean T" > , "histonet@lists.utsouthwestern.edu" > > Message-ID: > > Content-Type: text/plain; charset="utf-8" > > The only special stain that I know that requires the use of a negative control is for the AFB. I understand to rule out false positives as the AFB bacteria might exist in tap water. Nevertheless, a good QA practice which we will implement now. > > Other than Carson, does anyone know of a regulatory or accreditation agency is requiring this as well? Any suggestion on a good control tissue type? Carson recommends uterus. Also if there is a pick up on the negative slide (link to the tap water) will use of distilled water and a repeat procedure fix this? > > Any thoughts from fellow histonetters? > > Thanks > Ian Bernard > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cls71877 <@t> gmail.com Thu Mar 14 17:36:35 2013 From: cls71877 <@t> gmail.com (Cristi Rigazio) Date: Thu Mar 14 17:36:54 2013 Subject: [Histonet] RE: AFB and negative control In-Reply-To: References: <47E9B2C01DDDD94881EACD2DC44EBC880157985C@D1PWPEXMBX05.mdanderson.edu> Message-ID: Isn't a gram stain the only special that requires a positive and negative control? Sent from my iPhone On Mar 14, 2013, at 1:51 PM, joelle weaver wrote: > This practice is listed as a QC measure for issues of cross contamination in the ASCP publication "Quality Management in Anatomic Pathology, Nakhleh, R. M.D. I have never had any issues that were persistant enough to warrant this measure myself, but it is one of the suggestions made under the section for use of control tissue/slides. > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC >> From: TNMayer@mdanderson.org >> To: histonet@lists.utsouthwestern.edu >> Date: Thu, 14 Mar 2013 17:52:39 +0000 >> Subject: [Histonet] RE: AFB and negative control >> >> While I don't use a negative control for the AFB, I will use distilled water throughout the procedure. Most of the time the water in the waterbath is distilled as well, to rule out contamination there as well. Make sure the waterbath has been disinfected. >> >> Toysha N. Mayer, MBA, HT (ASCP) >> Instructor, Education Coordinator >> Program in Histotechnology >> School of Health Professions >> MD Anderson Cancer Center >> (713) 563-3481 >> tnmayer@mdanderson.org >> >> >> >> >> Date: Thu, 14 Mar 2013 12:42:30 +0000 >> From: Ian R Bernard >> Subject: [Histonet] AFB and Negative Control >> To: Lee & Peggy Wenk , "Tighe, Sean T" >> , "histonet@lists.utsouthwestern.edu" >> >> Message-ID: >> >> Content-Type: text/plain; charset="utf-8" >> >> The only special stain that I know that requires the use of a negative control is for the AFB. I understand to rule out false positives as the AFB bacteria might exist in tap water. Nevertheless, a good QA practice which we will implement now. >> >> Other than Carson, does anyone know of a regulatory or accreditation agency is requiring this as well? Any suggestion on a good control tissue type? Carson recommends uterus. Also if there is a pick up on the negative slide (link to the tap water) will use of distilled water and a repeat procedure fix this? >> >> Any thoughts from fellow histonetters? >> >> Thanks >> Ian Bernard >> >> >> >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tony.henwood <@t> health.nsw.gov.au Thu Mar 14 18:28:44 2013 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Thu Mar 14 18:29:25 2013 Subject: [Histonet] Re: Histonet Digest, Vol 112, Issue 13 In-Reply-To: References: <201303121703.r2CH38Dg002213@imp6.kp.org> Message-ID: <6D6BD1DE8A5571489398B392A38A71579D237318@xmdb04.nch.kids> Marilyn' Dandruff is believed to be the main culprit (a gem from the late (and Great) Lee Luna - we miss him). Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marilyn.A.Weiss@kp.org Sent: Friday, 15 March 2013 6:32 AM To: histonet@lists.utsouthwestern.edu Cc: histonet-bounces@lists.utsouthwestern.edu Subject: [Histonet] Re: Histonet Digest, Vol 112, Issue 13 Have a question to ask the group. One of the Pathologists is complaining about anucleate squames on her breast biopsies. Blames the tech for fingers being in the water bath, which may or may not happen. If she recuts the case it is great and she does not do anything different. what could cause this phenomenon? molds? It is driving us crazy. 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From: histonet-request@lists.utsouthwestern.edu To: histonet@lists.utsouthwestern.edu Date: 03/12/2013 10:03 AM Subject: Histonet Digest, Vol 112, Issue 13 Sent by: histonet-bounces@lists.utsouthwestern.edu Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Fw: news (Kim Donadio) 2. Detecting monoclonas in mouse lung (Leyva-Grado, Victor) 3. Reagent containers for VIP 3000 (Vickroy, Jim) ---------------------------------------------------------------------- Message: 1 Date: Mon, 11 Mar 2013 13:47:55 -0400 From: Kim Donadio Subject: Re: [Histonet] Fw: news To: Kim Donadio Cc: histonet , confirm unsub 2m2s2bpqnls4gcuu00bqawlkaga4rn0q , Pathrm35 , PensacolaToyBreedAdoption unsubscribe Message-ID: Content-Type: text/plain; charset=us-ascii Disregard something is up with my account. Sent from my iPhone On Mar 11, 2013, at 11:38 AM, Kim Donadio wrote: > http://www.britvoice.co.uk/wwvc/woszquloax/hkjmnqu=bvdzq > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Mon, 11 Mar 2013 19:37:29 +0000 From: "Leyva-Grado, Victor" Subject: [Histonet] Detecting monoclonas in mouse lung To: "'histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain; charset="us-ascii" Dear all, I have a couple of monoclonal antibodies raised against a respiratory virus. I used as treatment for disease and they worked. In trying to elucidate some mechanisms, I'm planning to biotinylate the antibody and at specific time points collect the lungs and do IHC to determine the distribution of the monoclonal. Do you think this will be feasible? I'm also planning to use some section for double labeling IF to determine the site of interaction antigen-antibody. Do you guys have any reference for this? Thanks a lot, Victor Victor H Leyva-Grado DVM, PhD Postdoctoral Fellow Microbiology Department Global Health and Emerging Pathogens Institute Icahn School of Medicine at Mount Sinai One Gustave L Levy Place Box 1124 Annenberg 16-15 New York, NY 10029 Phone 1-212-241-7094 Fax 1-212-534-1684 ------------------------------ Message: 3 Date: Tue, 12 Mar 2013 08:11:09 -0500 From: "Vickroy, Jim" Subject: [Histonet] Reagent containers for VIP 3000 To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" We have two old VIP 3000's and are trying to keep them running. Unfortunately finding some parts are getting g very hard. Can anyone steer me to who might have some old reagent containers for the VIP 3000? We have one container that started leaking yesterday and I suspect this is going to be an ongoing problem because of the age of the instrument. Thanks for your assistance. Jim James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 112, Issue 13 ***************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From G.Spoelstra <@t> murdoch.edu.au Fri Mar 15 03:55:59 2013 From: G.Spoelstra <@t> murdoch.edu.au (Gerard Spoelstra) Date: Fri Mar 15 08:27:41 2013 Subject: [Histonet] EMA,clone 23 dog Message-ID: Hi everyone, My colleague has been trying without success to run the monoclonal EMA , clone23 on a dog. He has tried ph9 tris buffer,ph 6 citrate/edta buffer and proteinase k for an hour using a 1/200 dilution and Dako envision. Any suggestions. Gerard Spoelstra Murdoch University From SHUNTER <@t> beaumont.edu Fri Mar 15 08:23:47 2013 From: SHUNTER <@t> beaumont.edu (Sue Hunter) Date: Fri Mar 15 16:57:18 2013 Subject: [Histonet] RE: SOS EGFRV3 In-Reply-To: <7B3DEB32E69C034EACB479059C5DE3FF75B795@EX-MLB-03.ad.georgiahealth.edu> References: <7B3DEB32E69C034EACB479059C5DE3FF75B795@EX-MLB-03.ad.georgiahealth.edu> Message-ID: Billie The last I heard (several years ago), this antibody was originally produced at a university - Duke maybe? They considered marketing it but then decided not to. I do not know of any other antibody for this mutation. We have a RT-PCR assay for it, but are not using it as there was no interest by our clinicians. If you ever find an antibody, please let me know. I would be interested in trying it also. Sue Sue Hunter, Supervisor Advanced Diagnostics Beaumont Health System Royal Oak MI 248-898-5146 shunter@beaumont.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Zimmerman, Billie Sent: Thursday, March 14, 2013 2:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] SOS EGFRV3 Does anyone perform this particular clone of EGFR?? Thanks in advance for your help. Billie Zimmerman MT(ASCP)QIHC 706-721-5617/3630 Augusta State University and Georgia Health Sciences University have consolidated to become Georgia Regents University. Effective January 9, 2013, my email address has changed to BZIMMERM@gru.edu. Please update your address book to reflect this change. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From shive003 <@t> umn.edu Fri Mar 15 09:32:05 2013 From: shive003 <@t> umn.edu (Jan Shivers) Date: Fri Mar 15 17:22:25 2013 Subject: [Histonet] EMA,clone 23 dog In-Reply-To: References: Message-ID: I have never had success in getting an EMA to cross-react with dog tissue (that particular clone epitope not conserved across species). If he has any success, I would be grateful to find out what antibody company and clone he uses. Best regards, Jan Shivers Senior Scientist University of Minnesota Veterinary Diagnostic Lab On Fri, Mar 15, 2013 at 3:55 AM, Gerard Spoelstra < G.Spoelstra@murdoch.edu.au> wrote: > Hi everyone, > > > > My colleague has been trying without success to run the monoclonal EMA , > clone23 on a dog. He has tried ph9 tris buffer,ph 6 citrate/edta buffer > and proteinase k for an hour using a 1/200 dilution and Dako envision. > > Any suggestions. > > > > Gerard Spoelstra > > Murdoch University > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jburch01 <@t> gmail.com Fri Mar 15 10:01:49 2013 From: jburch01 <@t> gmail.com (Jim Burchette) Date: Fri Mar 15 17:28:17 2013 Subject: [Histonet] RE: SOS EGFRV3 In-Reply-To: References: <7B3DEB32E69C034EACB479059C5DE3FF75B795@EX-MLB-03.ad.georgiahealth.edu> Message-ID: Sue, you are correct, EGFR v III was developed at Duke by Dr Darell Bigner. BA, you can reach him at 929-684-5018. Email is bigner@duke.edu I ran this antibody when I was there. Email me for IHC specifics. Best, JB On Mar 15, 2013 9:25 AM, "Sue Hunter" wrote: > Billie > The last I heard (several years ago), this antibody was originally > produced at a university - Duke maybe? They considered marketing it but > then decided not to. I do not know of any other antibody for this > mutation. We have a RT-PCR assay for it, but are not using it as there was > no interest by our clinicians. If you ever find an antibody, please let me > know. I would be interested in trying it also. > Sue > > Sue Hunter, Supervisor > Advanced Diagnostics > Beaumont Health System > Royal Oak MI > 248-898-5146 > shunter@beaumont.edu > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Zimmerman, Billie > Sent: Thursday, March 14, 2013 2:39 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] SOS EGFRV3 > > Does anyone perform this particular clone of EGFR?? Thanks in advance for > your help. > > > Billie Zimmerman MT(ASCP)QIHC > > 706-721-5617/3630 > > > Augusta State University and Georgia Health Sciences University have > consolidated to become Georgia Regents University. Effective January 9, > 2013, my email address has changed to BZIMMERM@gru.edu. Please update > your address book to reflect this change. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From allyse124 <@t> gmail.com Fri Mar 15 10:30:14 2013 From: allyse124 <@t> gmail.com (Allyse Mazzarelli) Date: Fri Mar 15 17:35:48 2013 Subject: [Histonet] Hematoxylin & Eosin-Y Re-staining Message-ID: Hi all you histonetters! I had stained a couple of slides yesterday using H&E, but the staining came out a little too light for my liking. Any set protocols for stripping the stain and redoing it? (I use 7100 plastic resin). I've put the slides in xylene to remove the coverslip/mounting medium, and if I cannot get a decent restain, I'll just cut more sections. I'm just curious if anyone has a protocol that works well. This would save me a lot of time! Thanks! Allyse Mazzarelli Histologist Neurotech USA Inc. From SHUNTER <@t> beaumont.edu Fri Mar 15 10:48:35 2013 From: SHUNTER <@t> beaumont.edu (Sue Hunter) Date: Fri Mar 15 17:39:53 2013 Subject: [Histonet] RE: SOS EGFRV3 In-Reply-To: References: <7B3DEB32E69C034EACB479059C5DE3FF75B795@EX-MLB-03.ad.georgiahealth.edu> Message-ID: Thanks for the update. sue From: Jim Burchette [mailto:jburch01@gmail.com] Sent: Friday, March 15, 2013 11:02 AM To: Sue Hunter Cc: Zimmerman, Billie; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: SOS EGFRV3 Sue, you are correct, EGFR v III was developed at Duke by Dr Darell Bigner. BA, you can reach him at 929-684-5018. Email is bigner@duke.edu I ran this antibody when I was there. Email me for IHC specifics. Best, JB On Mar 15, 2013 9:25 AM, "Sue Hunter" > wrote: Billie The last I heard (several years ago), this antibody was originally produced at a university - Duke maybe? They considered marketing it but then decided not to. I do not know of any other antibody for this mutation. We have a RT-PCR assay for it, but are not using it as there was no interest by our clinicians. If you ever find an antibody, please let me know. I would be interested in trying it also. Sue Sue Hunter, Supervisor Advanced Diagnostics Beaumont Health System Royal Oak MI 248-898-5146 shunter@beaumont.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Zimmerman, Billie Sent: Thursday, March 14, 2013 2:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] SOS EGFRV3 Does anyone perform this particular clone of EGFR?? Thanks in advance for your help. Billie Zimmerman MT(ASCP)QIHC 706-721-5617/3630 Augusta State University and Georgia Health Sciences University have consolidated to become Georgia Regents University. Effective January 9, 2013, my email address has changed to BZIMMERM@gru.edu. Please update your address book to reflect this change. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From debgranato <@t> yahoo.com Fri Mar 15 11:00:19 2013 From: debgranato <@t> yahoo.com (Debbie Granato) Date: Fri Mar 15 17:41:39 2013 Subject: [Histonet] Billing for Pin 4 Cocktail Message-ID: <1363363219.67860.YahooMailClassic@web161202.mail.bf1.yahoo.com> We?have a billing?question for the PIN4 Cocktail that we perform on prostate needle biopsies. We currently use the Biocare CK5+CK14+p63 and then add the Biocare?P?504S in the lab. How would you bill for this? ?Would billing for 1 stain be correct or can you bill for 3 stains? ? Thank you for your input. Debbie Granato ? From rheyna <@t> lumc.edu Fri Mar 15 11:09:01 2013 From: rheyna <@t> lumc.edu (Roger Heyna) Date: Fri Mar 15 17:44:32 2013 Subject: [Histonet] Billing for Pin 4 Cocktail In-Reply-To: <1363363219.67860.YahooMailClassic@web161202.mail.bf1.yahoo.com> References: <1363363219.67860.YahooMailClassic@web161202.mail.bf1.yahoo.com> Message-ID: <5143014D020000230004BE7C@gwgwia1.luhs.org> Our lab currently bills for three, and I know of other labs in our area that also bill for three. I have heard rumors that this may not be allowed in the near future. Roger Heyna Maywood, IL >>> Debbie Granato 3/15/2013 11:00 AM >>> We have a billing question for the PIN4 Cocktail that we perform on prostate needle biopsies. We currently use the Biocare CK5+CK14+p63 and then add the Biocare P 504S in the lab. How would you bill for this? Would billing for 1 stain be correct or can you bill for 3 stains? Thank you for your input. Debbie Granato _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mbmphoto <@t> gmail.com Fri Mar 15 11:11:52 2013 From: mbmphoto <@t> gmail.com (Maria Mejia) Date: Fri Mar 15 17:44:34 2013 Subject: [Histonet] searching for a good microtome repair person Message-ID: Please, can anyone recommend a good microtome repair person in the Bay Area (San Francisco/San Jose, CA). Please provide contact information. Maria Mejia Affymetric, Inc Santa Clara, CA From Rcartun <@t> harthosp.org Fri Mar 15 11:34:31 2013 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Mar 15 17:51:33 2013 Subject: [Histonet] PCR detection for Mycobacteria Message-ID: <51431556.7770.0077.1@harthosp.org> Is anyone offering clinical PCR detection for Mycobacteria from formalin-fixed, paraffin-embedded tissue? Thanks! Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-2204 Fax From vperez <@t> pathreflab.com Fri Mar 15 11:42:44 2013 From: vperez <@t> pathreflab.com (Vanessa Perez) Date: Fri Mar 15 17:52:45 2013 Subject: [Histonet] Billing for Pin 4 Cocktail References: <1363363219.67860.YahooMailClassic@web161202.mail.bf1.yahoo.com> <5143014D020000230004BE7C@gwgwia1.luhs.org> Message-ID: Its actually not allowed already. Any cocktail where all stains are done at one time on one slide can only be charged x1 even if its 2 or more antibodys in the cocktail. One of our reps updated us with our PIN cocktail -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Roger Heyna Sent: Friday, March 15, 2013 11:09 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Billing for Pin 4 Cocktail Our lab currently bills for three, and I know of other labs in our area that also bill for three. I have heard rumors that this may not be allowed in the near future. Roger Heyna Maywood, IL >>> Debbie Granato 3/15/2013 11:00 AM >>> We have a billing question for the PIN4 Cocktail that we perform on prostate needle biopsies. We currently use the Biocare CK5+CK14+p63 and then add the Biocare P 504S in the lab. How would you bill for this? Would billing for 1 stain be correct or can you bill for 3 stains? Thank you for your input. Debbie Granato _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lcolbert <@t> pathmdlabs.com Fri Mar 15 11:51:27 2013 From: lcolbert <@t> pathmdlabs.com (Laurie Colbert) Date: Fri Mar 15 17:56:13 2013 Subject: [Histonet] IHC validation Message-ID: <12ECD7346266D74691EC2BFC75285E451CC048EB@BFL323E10.pathmdlabs.local> If a lab is just starting up IHC for the first time and has to validate both the IHC stainer and the AB's/detection kit, does the validation have to be done using actual patient cases - or can you use strictly control tissue and purchased microarrays? Laurie Colbert, HT (ASCP) From Timothy.Morken <@t> ucsfmedctr.org Fri Mar 15 12:05:22 2013 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Fri Mar 15 17:58:35 2013 Subject: [Histonet] RE: IHC validation In-Reply-To: <12ECD7346266D74691EC2BFC75285E451CC048EB@BFL323E10.pathmdlabs.local> References: <12ECD7346266D74691EC2BFC75285E451CC048EB@BFL323E10.pathmdlabs.local> Message-ID: <761E2B5697F795489C8710BCC72141FF0646B1@ex07.net.ucsf.edu> Laurie, for first time validation use controls. Don't use patient cases that are done for initial diagnostics. If they are repeats to test the system, that is ok. The question is, who tested the controls? Ideally you will send a representative sample of your slides to another lab that uses the same or similar reagents to run for parallel testing. Tim Morken Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Friday, March 15, 2013 9:51 AM To: Histonet Post (histonet@lists.utsouthwestern.edu) Subject: [Histonet] IHC validation If a lab is just starting up IHC for the first time and has to validate both the IHC stainer and the AB's/detection kit, does the validation have to be done using actual patient cases - or can you use strictly control tissue and purchased microarrays? Laurie Colbert, HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wdesalvo.cac <@t> outlook.com Fri Mar 15 12:24:08 2013 From: wdesalvo.cac <@t> outlook.com (WILLIAM DESALVO) Date: Fri Mar 15 18:02:06 2013 Subject: [Histonet] IHC validation In-Reply-To: <12ECD7346266D74691EC2BFC75285E451CC048EB@BFL323E10.pathmdlabs.local> References: <12ECD7346266D74691EC2BFC75285E451CC048EB@BFL323E10.pathmdlabs.local> Message-ID: Laurie, I would use the control slides to validate and set up the antibodies. I good secondary check/control would be to send slides to another lab to check for correlation and confirmation of your protocol performance. Once you get an established process, then you can later check patient samples to the protocol and make adjustments, as necessary. William DeSalvo, BS HTL(ASCP) Production Manager-Anatomic Pathology Chair, NSH Quality Management Committee Owner/Consultant, Collaborative Advantage Consulting > From: lcolbert@pathmdlabs.com > To: histonet@lists.utsouthwestern.edu > Date: Fri, 15 Mar 2013 16:51:27 +0000 > Subject: [Histonet] IHC validation > > If a lab is just starting up IHC for the first time and has to validate both the IHC stainer and the AB's/detection kit, does the validation have to be done using actual patient cases - or can you use strictly control tissue and purchased microarrays? > > Laurie Colbert, HT (ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From turkekul <@t> gmail.com Fri Mar 15 12:35:25 2013 From: turkekul <@t> gmail.com (Mesru T) Date: Fri Mar 15 18:03:20 2013 Subject: [Histonet] Thawing frozen tissue Message-ID: Hi Maria, Often people freeze the tissues right after dissecting them out of the animal because either they are not sure what to do or they do not know better. Then they bring the frozen tissues kept at -80 C freezer and request paraffin embedding. I usually prefer to thaw them gradually. I place the frozen tissues in the cryostat (in a tightly sealed zip lock bag), which is at -50 C. Then I increase the temperature of the cryostat by 5 C every 45 min minutes until it is -5 C. Then I prepare ice cold 10% NBF (or 4% PFA) and place the tissues in the ice cold fixative and let the tissues fix at room temperature for enough time depending on the size and the type of tissue (For my tissues I fixed overnight at room temp). After that I process for paraffin embedding. The H&E staining comes fine for frozen tissue. And I have tried IHC with 4 different antibodies and had excellent results. I have tried mouse brain tumor ( pieces like ~1cm3 in volume) , human brain tumor ( small pieces like~ 0.5cm3 in volume) and human lymph nodes (pieces like ~3mm3 in volume) with good results. You can also re-freeze after thawing but you may have poor tissue architecture. I would suggest you to do a pilot trial experiment. You can thaw one block that you can spare (or you can prepare a new samples just to test thawing) and test how it comes out for your particular case. If you have satisfactory results you can go ahead and thaw other samples. I have tried to section frozen gelatin embedded tissue in the past and it was very difficult. Please take images with your phone and send us the photos of the defective blocks. Good luck! Mesru From wbenton <@t> cua.md Fri Mar 15 12:43:33 2013 From: wbenton <@t> cua.md (Walter Benton) Date: Fri Mar 15 18:04:30 2013 Subject: [Histonet] Freezing Microtome Special Stains Message-ID: <0B8979A204680A42B93A52B486088CD934229102DB@CUAEXH1.GCU-MD.local> We have an employee/student that is working on the instrumentation portion of her coursework and came across the Clinical Freezing Microtome (mostly replaced by a cryostat) Carson 3rd ed. pg. 58 . She would like to know if anyone has more information on the types of special stains that require free floating sections using this instrument and if anyone has pictures or links to documentation on the instrument. Thanks Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 126 (All Deliveries to Suite 127) Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) wbenton@cua.md ________________________________ CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From BGapinski <@t> pathgroup.com Fri Mar 15 13:29:54 2013 From: BGapinski <@t> pathgroup.com (Bruce Gapinski) Date: Fri Mar 15 18:13:51 2013 Subject: [Histonet] Microtome repair Message-ID: Jeff Myers is the best I've seen in my 35 years. This guy will cut beautiful sections as his final test after repair. He's one of "us". (408) 469-0957 Bruce Gapinsk HT (ASCP) Chief Histologist Marin Medical Laboratories PathGroup SF ________________________________ Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you From DSiena <@t> statlab.com Fri Mar 15 14:10:33 2013 From: DSiena <@t> statlab.com (Debra Siena) Date: Fri Mar 15 18:21:43 2013 Subject: [Histonet] RE: AFB and negative control In-Reply-To: References: <47E9B2C01DDDD94881EACD2DC44EBC880157985C@D1PWPEXMBX05.mdanderson.edu> Message-ID: Hi All, According to CLIA Interpretative guidelines, a negative AFB control tissue is to be run each day of testing. ?493.1256 Standard: Control procedures. (e)(2) Each day of use (unless otherwise specified in this subpart), test staining materials for intended reactivity to ensure predictable staining characteristics. Control materials for both positive and negative reactivity must be included, as appropriate. Interpretive Guidelines ?493.1256(e)(2)-(e)(3) Acid-fast stains must be checked each day of use for positive and negative reactivity. Debbie Siena 800.442.3573 ext. 229 | www.statlab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelle weaver Sent: Thursday, March 14, 2013 3:51 PM To: Mayer,Toysha N; 'histonet@lists.utsouthwestern.edu' Subject: RE: [Histonet] RE: AFB and negative control This practice is listed as a QC measure for issues of cross contamination in the ASCP publication "Quality Management in Anatomic Pathology, Nakhleh, R. M.D. I have never had any issues that were persistant enough to warrant this measure myself, but it is one of the suggestions made under the section for use of control tissue/slides. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: TNMayer@mdanderson.org > To: histonet@lists.utsouthwestern.edu > Date: Thu, 14 Mar 2013 17:52:39 +0000 > Subject: [Histonet] RE: AFB and negative control > > While I don't use a negative control for the AFB, I will use distilled water throughout the procedure. Most of the time the water in the waterbath is distilled as well, to rule out contamination there as well. Make sure the waterbath has been disinfected. > > Toysha N. Mayer, MBA, HT (ASCP) > Instructor, Education Coordinator > Program in Histotechnology > School of Health Professions > MD Anderson Cancer Center > (713) 563-3481 > tnmayer@mdanderson.org > > > > > Date: Thu, 14 Mar 2013 12:42:30 +0000 > From: Ian R Bernard > Subject: [Histonet] AFB and Negative Control > To: Lee & Peggy Wenk , "Tighe, Sean T" > , "histonet@lists.utsouthwestern.edu" > > Message-ID: > > Content-Type: text/plain; charset="utf-8" > > The only special stain that I know that requires the use of a negative control is for the AFB. I understand to rule out false positives as the AFB bacteria might exist in tap water. Nevertheless, a good QA practice which we will implement now. > > Other than Carson, does anyone know of a regulatory or accreditation agency is requiring this as well? Any suggestion on a good control tissue type? Carson recommends uterus. Also if there is a pick up on the negative slide (link to the tap water) will use of distilled water and a repeat procedure fix this? > > Any thoughts from fellow histonetters? > > Thanks > Ian Bernard > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From baazaouinarjes <@t> gmail.com Fri Mar 15 15:21:48 2013 From: baazaouinarjes <@t> gmail.com (narjes baazaoui) Date: Fri Mar 15 18:35:55 2013 Subject: [Histonet] vibratome mice brain sectionning problem Message-ID: Hello, I used to section the brains of my mice by microtome I did not have any issue. This period I changed to the vibratome I notice that it is cutting whole sections at the beginning then it is cutting just parts of the brain such as the hippocampus and parietal cortex without the frontal cortex or even just the hippocampus without the other structures. I am using cold PBS and sharp blade and the angle of the blade is 5 degree. If any body had any experience before with the vibratome or encounter this problem please do not hesitate to help me. Thank you so much. Regards. Narjes Baazaoui -- Narjes Baazaoui, PhD student Institute of Basic research (IBR), Laboratory of chemical neuropathology 3475995601 From RDELAVEGAAMADOR <@t> PARTNERS.ORG Fri Mar 15 15:41:23 2013 From: RDELAVEGAAMADOR <@t> PARTNERS.ORG (De La Vega Amador, Rodolfo Enrique) Date: Fri Mar 15 18:39:33 2013 Subject: [Histonet] Bone processing help Message-ID: <0F62C13F3FBAE541AC2D6AE56E26E85041D30F@PHSX10MB15.partners.org> Hi everybody, I am fairly new to all Histotechnology processes. We mainly work in our lab with mineralized bone with implants, so we fix them, dehydrate them, embed them in MMA, cut them, glued them to slides, ground them manually and then stain them. I need help in some parts of the process that need improvement: 1. We use Loctite 4471 on the samples, put them under vacuum, apply them to the slide and more vacuum. There's good results but there are some bubbles that still appear. Suggestions? 2. I can't seem to get the yellow/orange on bone with the Van Gieson stain. I've been doinga preheat at 55 ?C, etching, rinse in DI water, Sanderson?s Rapid Bone Stain, running tap water, Van Gieson (commercial from DHM) for 30 seconds to 5 minutes, paper dry and quickly dehydrate with 100% EtOH. What am I doing wrong? 3. The stain's been running when I add the glue to cover slip the slides. Especially the green color. Why does this happen? 4. Cover slipping is done with cyano acrylate, under suction as before, but bubbles still appear. I've tried placing the cover slip on an angle and wait for the glue to evenly spread, but the bone has just too many pores and air bubbles appear. Please help. I greatly appreciate any help on the subject. Best, Rodolfo De la Vega, MD. Research Fellow Laboratory for Musculoskeletal Research and Innovation Massachusetts General Hospital - Jackson Building 1120 The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From claudiamel2000 <@t> yahoo.com Sat Mar 16 03:27:11 2013 From: claudiamel2000 <@t> yahoo.com (claudia melidona) Date: Sat Mar 16 03:29:07 2013 Subject: [Histonet] FW: No Subject Message-ID: <1363422431.86984.YahooMailNeo@web162104.mail.bf1.yahoo.com> http://www.christiankeltermann.de/kqbc/kzcgvmemedmii.roplf From gu.lang <@t> gmx.at Sat Mar 16 04:42:33 2013 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sat Mar 16 04:43:51 2013 Subject: AW: [Histonet] PCR detection for Mycobacteria In-Reply-To: <51431556.7770.0077.1@harthosp.org> References: <51431556.7770.0077.1@harthosp.org> Message-ID: <001501ce222a$9667a9f0$c336fdd0$@gmx.at> I'm also interested in these assays. BioProducts in Austria sells a kit vor LightCycler PCR and they state it's good for FFPE. But on their website one cannot see the details. It's for 96 tests. I've found a 24-kit from Qiagen, which should work on FFPE. But the assay is not validated for it. Regards Gudrun Lang -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Richard Cartun Gesendet: Freitag, 15. M?rz 2013 17:35 An: Histonet Betreff: [Histonet] PCR detection for Mycobacteria Is anyone offering clinical PCR detection for Mycobacteria from formalin-fixed, paraffin-embedded tissue? Thanks! Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-2204 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Ashley.Troutman <@t> Vanderbilt.Edu Sat Mar 16 09:41:21 2013 From: Ashley.Troutman <@t> Vanderbilt.Edu (Troutman, Kenneth A) Date: Sat Mar 16 09:42:19 2013 Subject: [Histonet] IHC validation Message-ID: Hi Laurie, Ideally, you would like to validate both with tissues processed in your laboratory (controls and patient tissue). Purchased TMAs are okay, but they should not be the only thing used. For the instrument validation, I would definitely use tissue that has been processed in your lab and run the same stain on each instrument (I think we ran serial sections of vimentin) and compare them very closely for any variation (use the same antibody and the same detection kit for each instrument). My recommendation for new antibody validations is to run a set of normal tissues and tumor tissues (we have about 15 normal tissues that we harvested and created our own sausage block) along with about 20 or so different tumors (also created in sausage blocks) to see how they stain under as many circumstances as possible (especially in the conditions your pathologists are most likely to see). In addition to that, run a set of known positives and known negatives from your cases. CAP recommends 10 of each, but I believe the final number is at the Medical Director's discretion. You can also run a representative sample of positives and negatives in your lab and send another set of the same tissues to another lab that has a validated procedure for that antibody and validate it that way. As far as the detection kit goes, we don't actually "validate" the detection kit per se. We do a lot-to-lot comparison, which involves running tissue from the same block (we created special blocks specifically for detection kits) and run the same antibody (AE1/AE3 or CD3--something we do a LOT of) to compare from lot to lot. I hope I answered your question. Regards, Ashley Troutman BS, HT(ASCP) QIHC Immunohistochemistry Supervisor Vanderbilt University Histopathology 1301 Medical Center Drive TVC 4531 Nashville, TN 37232 ashley.troutman@vanderbilt.edu Message: 21 Date: Fri, 15 Mar 2013 16:51:27 +0000 From: Laurie Colbert > Subject: [Histonet] IHC validation To: "Histonet Post (histonet@lists.utsouthwestern.edu)" > Message-ID: <12ECD7346266D74691EC2BFC75285E451CC048EB@BFL323E10.pathmdlabs.local> Content-Type: text/plain; charset="us-ascii" If a lab is just starting up IHC for the first time and has to validate both the IHC stainer and the AB's/detection kit, does the validation have to be done using actual patient cases - or can you use strictly control tissue and purchased microarrays? Laurie Colbert, HT (ASCP) From kiran_g <@t> sbcglobal.net Sat Mar 16 10:39:06 2013 From: kiran_g <@t> sbcglobal.net (Kiranjit Grewal) Date: Sat Mar 16 10:39:21 2013 Subject: [Histonet] Best Carmine stain Message-ID: <8D2ECF37-6661-478D-886C-566493FDA66A@sbcglobal.net> Hello, Any tips on doing Best Carmine on Frozen sections without water. Can we use mucicarmine solution to pick up glycogen in heart muscle? Thank u, Kiran Sent from my iPhone From one_angel_secret <@t> yahoo.com Sat Mar 16 11:07:08 2013 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Sat Mar 16 11:07:48 2013 Subject: [Histonet] RE: IHC validation In-Reply-To: <761E2B5697F795489C8710BCC72141FF0646B1@ex07.net.ucsf.edu> References: <12ECD7346266D74691EC2BFC75285E451CC048EB@BFL323E10.pathmdlabs.local> <761E2B5697F795489C8710BCC72141FF0646B1@ex07.net.ucsf.edu> Message-ID: <1363450028.62436.YahooMailNeo@web161601.mail.bf1.yahoo.com> I agree about using known controls however sending cases out for comparison?could be a little costly, it is ideal though. One thing i have done is to go back in the records and pull up old cases that have been sent out for IHC and use those to compare not only the test as a case corrolation but also to compare any new controls I might want to continue using. Your pathologist should be able to verify accuracy and sign off on that.?? ? As far as the controls, Id purchase known controls those usually come with a stained example so you can compare that with your new controls and?any old known?cases you may have. Cases( blocks)?that are past thier?legal holding date of course.?? ? Good luck!! ? Kim D ? ? ? ________________________________ From: "Morken, Timothy" To: Laurie Colbert ; "Histonet Post (histonet@lists.utsouthwestern.edu)" Sent: Friday, March 15, 2013 1:05 PM Subject: [Histonet] RE: IHC validation Laurie, for first time validation use controls. Don't use patient cases that are done for initial diagnostics. If they are repeats to test the system, that is ok. The question is, who tested the controls? Ideally you will send a representative sample of your slides to another lab that uses the same or similar reagents to run for parallel testing. Tim Morken Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Friday, March 15, 2013 9:51 AM To: Histonet Post (histonet@lists.utsouthwestern.edu) Subject: [Histonet] IHC validation If a lab is just starting up IHC for the first time and has to validate both the IHC stainer and the AB's/detection kit, does the validation have to be done using actual patient cases - or can you use strictly control tissue and purchased microarrays? Laurie Colbert, HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From m.kap.1 <@t> erasmusmc.nl Sat Mar 16 12:07:53 2013 From: m.kap.1 <@t> erasmusmc.nl (M. Kap) Date: Sat Mar 16 12:08:01 2013 Subject: [Histonet] Re: Histonet Digest, Vol 112, Issue 17 ihc validation In-Reply-To: References: Message-ID: <23BA09B2-C2CD-4102-87D8-55FBF82C9736@erasmusmc.nl> Check out www.cqpath.com, nice references from a2z ;-) Verstuurd vanaf mijn iPad Op 16 mrt. 2013 om 18:05 heeft "histonet-request@lists.utsouthwestern.edu" het volgende geschreven: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. RE: IHC validation (Morken, Timothy) > 2. RE: IHC validation (WILLIAM DESALVO) > 3. Thawing frozen tissue (Mesru T) > 4. Freezing Microtome Special Stains (Walter Benton) > 5. Microtome repair (Bruce Gapinski) > 6. RE: RE: AFB and negative control (Debra Siena) > 7. vibratome mice brain sectionning problem (narjes baazaoui) > 8. Bone processing help (De La Vega Amador, Rodolfo Enrique) > 9. FW: No Subject (claudia melidona) > 10. AW: [Histonet] PCR detection for Mycobacteria (Gudrun Lang) > 11. RE: IHC validation (Troutman, Kenneth A) > 12. Best Carmine stain (Kiranjit Grewal) > 13. Re: RE: IHC validation (Kim Donadio) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Fri, 15 Mar 2013 17:05:22 +0000 > From: "Morken, Timothy" > Subject: [Histonet] RE: IHC validation > To: "Laurie Colbert" , "Histonet Post > (histonet@lists.utsouthwestern.edu)" > > Message-ID: <761E2B5697F795489C8710BCC72141FF0646B1@ex07.net.ucsf.edu> > Content-Type: text/plain; charset=us-ascii > > Laurie, for first time validation use controls. Don't use patient cases that are done for initial diagnostics. If they are repeats to test the system, that is ok. > > The question is, who tested the controls? Ideally you will send a representative sample of your slides to another lab that uses the same or similar reagents to run for parallel testing. > > Tim Morken > Department of Pathology > UC San Francisco Medical Center > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert > Sent: Friday, March 15, 2013 9:51 AM > To: Histonet Post (histonet@lists.utsouthwestern.edu) > Subject: [Histonet] IHC validation > > If a lab is just starting up IHC for the first time and has to validate both the IHC stainer and the AB's/detection kit, does the validation have to be done using actual patient cases - or can you use strictly control tissue and purchased microarrays? > > Laurie Colbert, HT (ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 2 > Date: Fri, 15 Mar 2013 10:24:08 -0700 > From: WILLIAM DESALVO > Subject: RE: [Histonet] IHC validation > To: Laurie Colbert , histonet > > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > Laurie, I would use the control slides to validate and set up the antibodies. I good secondary check/control would be to send slides to another lab to check for correlation and confirmation of your protocol performance. Once you get an established process, then you can later check patient samples to the protocol and make adjustments, as necessary. > > William DeSalvo, BS HTL(ASCP) > Production Manager-Anatomic Pathology > Chair, NSH Quality Management Committee > Owner/Consultant, Collaborative Advantage Consulting > > > >> From: lcolbert@pathmdlabs.com >> To: histonet@lists.utsouthwestern.edu >> Date: Fri, 15 Mar 2013 16:51:27 +0000 >> Subject: [Histonet] IHC validation >> >> If a lab is just starting up IHC for the first time and has to validate both the IHC stainer and the AB's/detection kit, does the validation have to be done using actual patient cases - or can you use strictly control tissue and purchased microarrays? >> >> Laurie Colbert, HT (ASCP) >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Message: 3 > Date: Fri, 15 Mar 2013 13:35:25 -0400 > From: Mesru T > Subject: [Histonet] Thawing frozen tissue > To: histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset=ISO-8859-1 > > Hi Maria, > > Often people freeze the tissues right after dissecting them out of the > animal because either they are not sure what to do or they do not know > better. > Then they bring the frozen tissues kept at -80 C freezer and request > paraffin embedding. > I usually prefer to thaw them gradually. I place the frozen tissues in the > cryostat (in a tightly sealed zip lock bag), which is at -50 C. Then I > increase the temperature of the cryostat by 5 C every 45 min minutes until > it is -5 C. Then I prepare ice cold 10% NBF (or 4% PFA) and place the > tissues in the ice cold fixative and let the tissues fix at room > temperature for enough time depending on the size and the type of tissue > (For my tissues I fixed overnight at room temp). After that I process for > paraffin embedding. The H&E staining comes fine for frozen tissue. And I > have tried IHC with 4 different antibodies and had excellent results. I > have tried mouse brain tumor ( pieces like ~1cm3 in volume) , human brain > tumor ( small pieces like~ 0.5cm3 in volume) and human lymph nodes (pieces > like ~3mm3 in volume) with good results. You can also re-freeze after > thawing but you may have poor tissue architecture. > I would suggest you to do a pilot trial experiment. You can thaw one block > that you can spare (or you can prepare a new samples just to test thawing) > and test how it comes out for your particular case. If you have > satisfactory results you can go ahead and thaw other samples. I have tried > to section frozen gelatin embedded tissue in the past and it was very > difficult. Please take images with your phone and send us the photos of the > defective blocks. > > Good luck! > Mesru > > > ------------------------------ > > Message: 4 > Date: Fri, 15 Mar 2013 13:43:33 -0400 > From: Walter Benton > Subject: [Histonet] Freezing Microtome Special Stains > To: "histonet@lists.utsouthwestern.edu" > > Cc: "Denicia A. Moore" > Message-ID: > <0B8979A204680A42B93A52B486088CD934229102DB@CUAEXH1.GCU-MD.local> > Content-Type: text/plain; charset="iso-8859-1" > > We have an employee/student that is working on the instrumentation portion of her coursework and came across the Clinical Freezing Microtome (mostly replaced by a cryostat) Carson 3rd ed. pg. 58 . She would like to know if anyone has more information on the types of special stains that require free floating sections using this instrument and if anyone has pictures or links to documentation on the instrument. > > Thanks > > Walter Benton HT(ASCP)QIHC > Histology Supervisor > Chesapeake Urology Associates > 806 Landmark Drive, Suite 126 > (All Deliveries to Suite 127) > Glen Burnie, MD 21061 > 443-471-5850 (Direct) > 410-768-5961 (Lab) > 410-768-5965 (Fax) > wbenton@cua.md > > > ________________________________ > CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. > > > ------------------------------ > > Message: 5 > Date: Fri, 15 Mar 2013 18:29:54 +0000 > From: "Bruce Gapinski" > Subject: [Histonet] Microtome repair > To: "'histonet@lists.utsouthwestern.edu'" > > Message-ID: > > Content-Type: text/plain; charset=us-ascii > > Jeff Myers is the best I've seen in my 35 years. This guy will cut beautiful sections as his final test after repair. He's one of "us". > (408) 469-0957 > > Bruce Gapinsk HT (ASCP) > Chief Histologist > Marin Medical Laboratories > PathGroup SF > > > ________________________________ > > Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you > > > ------------------------------ > > Message: 6 > Date: Fri, 15 Mar 2013 14:10:33 -0500 > From: Debra Siena > Subject: RE: [Histonet] RE: AFB and negative control > To: joelle weaver , "Mayer,Toysha N" > , "'histonet@lists.utsouthwestern.edu'" > > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > Hi All, > > According to CLIA Interpretative guidelines, a negative AFB control tissue is to be run each day of testing. > > ?493.1256 Standard: Control procedures. > > (e)(2) Each day of use (unless otherwise specified in this subpart), test staining materials for intended reactivity to ensure predictable staining characteristics. Control materials for both positive and negative reactivity must be included, as appropriate. > > Interpretive Guidelines ?493.1256(e)(2)-(e)(3) > Acid-fast stains must be checked each day of use for positive and negative reactivity. > > > > Debbie Siena > 800.442.3573 ext. 229 | www.statlab.com > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelle weaver > Sent: Thursday, March 14, 2013 3:51 PM > To: Mayer,Toysha N; 'histonet@lists.utsouthwestern.edu' > Subject: RE: [Histonet] RE: AFB and negative control > > This practice is listed as a QC measure for issues of cross contamination in the ASCP publication "Quality Management in Anatomic Pathology, Nakhleh, R. M.D. I have never had any issues that were persistant enough to warrant this measure myself, but it is one of the suggestions made under the section for use of control tissue/slides. > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC >> From: TNMayer@mdanderson.org >> To: histonet@lists.utsouthwestern.edu >> Date: Thu, 14 Mar 2013 17:52:39 +0000 >> Subject: [Histonet] RE: AFB and negative control >> >> While I don't use a negative control for the AFB, I will use distilled water throughout the procedure. Most of the time the water in the waterbath is distilled as well, to rule out contamination there as well. Make sure the waterbath has been disinfected. >> >> Toysha N. Mayer, MBA, HT (ASCP) >> Instructor, Education Coordinator >> Program in Histotechnology >> School of Health Professions >> MD Anderson Cancer Center >> (713) 563-3481 >> tnmayer@mdanderson.org >> >> >> >> >> Date: Thu, 14 Mar 2013 12:42:30 +0000 >> From: Ian R Bernard >> Subject: [Histonet] AFB and Negative Control >> To: Lee & Peggy Wenk , "Tighe, Sean T" >> , "histonet@lists.utsouthwestern.edu" >> >> Message-ID: >> >> Content-Type: text/plain; charset="utf-8" >> >> The only special stain that I know that requires the use of a negative control is for the AFB. I understand to rule out false positives as the AFB bacteria might exist in tap water. Nevertheless, a good QA practice which we will implement now. >> >> Other than Carson, does anyone know of a regulatory or accreditation agency is requiring this as well? Any suggestion on a good control tissue type? Carson recommends uterus. Also if there is a pick up on the negative slide (link to the tap water) will use of distilled water and a repeat procedure fix this? >> >> Any thoughts from fellow histonetters? >> >> Thanks >> Ian Bernard >> >> >> >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 7 > Date: Fri, 15 Mar 2013 16:21:48 -0400 > From: narjes baazaoui > Subject: [Histonet] vibratome mice brain sectionning problem > To: histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset=ISO-8859-1 > > Hello, > I used to section the brains of my mice by microtome I did not have any > issue. This period I changed to the vibratome I notice that it is cutting > whole sections at the beginning then it is cutting just parts of the brain > such as the hippocampus and parietal cortex without the frontal cortex or > even just the hippocampus without the other structures. I am using cold PBS > and sharp blade and the angle of the blade is 5 degree. If any body had any > experience before with the vibratome or encounter this problem please do > not hesitate to help me. > Thank you so much. > Regards. > Narjes Baazaoui > -- > Narjes Baazaoui, PhD student > Institute of Basic research (IBR), > Laboratory of chemical neuropathology > 3475995601 > > > ------------------------------ > > Message: 8 > Date: Fri, 15 Mar 2013 20:41:23 +0000 > From: "De La Vega Amador, Rodolfo Enrique" > > Subject: [Histonet] Bone processing help > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > <0F62C13F3FBAE541AC2D6AE56E26E85041D30F@PHSX10MB15.partners.org> > Content-Type: text/plain; charset="iso-8859-1" > > Hi everybody, > > I am fairly new to all Histotechnology processes. We mainly work in our lab with mineralized bone with implants, so we fix them, dehydrate them, embed them in MMA, cut them, glued them to slides, ground them manually and then stain them. I need help in some parts of the process that need improvement: > > > 1. We use Loctite 4471 on the samples, put them under vacuum, apply them to the slide and more vacuum. There's good results but there are some bubbles that still appear. Suggestions? > 2. I can't seem to get the yellow/orange on bone with the Van Gieson stain. I've been doinga preheat at 55 ?C, etching, rinse in DI water, Sanderson?s Rapid Bone Stain, running tap water, Van Gieson (commercial from DHM) for 30 seconds to 5 minutes, paper dry and quickly dehydrate with 100% EtOH. What am I doing wrong? > 3. The stain's been running when I add the glue to cover slip the slides. Especially the green color. Why does this happen? > 4. Cover slipping is done with cyano acrylate, under suction as before, but bubbles still appear. I've tried placing the cover slip on an angle and wait for the glue to evenly spread, but the bone has just too many pores and air bubbles appear. Please help. > > I greatly appreciate any help on the subject. > > Best, > > Rodolfo De la Vega, MD. > Research Fellow > Laboratory for Musculoskeletal Research and Innovation > Massachusetts General Hospital - Jackson Building 1120 > > > > The information in this e-mail is intended only for the person to whom it is > addressed. If you believe this e-mail was sent to you in error and the e-mail > contains patient information, please contact the Partners Compliance HelpLine at > http://www.partners.org/complianceline . If the e-mail was sent to you in error > but does not contain patient information, please contact the sender and properly > dispose of the e-mail. > > > > > ------------------------------ > > Message: 9 > Date: Sat, 16 Mar 2013 01:27:11 -0700 (PDT) > From: claudia melidona > Subject: [Histonet] FW: No Subject > To: Bruce Dunlap , Gregg Hill > , Staci , gary > weidman , histonet request > , histonet > > Message-ID: > <1363422431.86984.YahooMailNeo@web162104.mail.bf1.yahoo.com> > Content-Type: text/plain; charset=us-ascii > > http://www.christiankeltermann.de/kqbc/kzcgvmemedmii.roplf > > > > ------------------------------ > > Message: 10 > Date: Sat, 16 Mar 2013 10:42:33 +0100 > From: "Gudrun Lang" > Subject: AW: [Histonet] PCR detection for Mycobacteria > To: "'Richard Cartun'" > Cc: Histonet@lists.utsouthwestern.edu > Message-ID: <001501ce222a$9667a9f0$c336fdd0$@gmx.at> > Content-Type: text/plain; charset="iso-8859-1" > > I'm also interested in these assays. > > BioProducts in Austria sells a kit vor LightCycler PCR and they state it's > good for FFPE. But on their website one cannot see the details. It's for 96 > tests. > I've found a 24-kit from Qiagen, which should work on FFPE. But the assay is > not validated for it. > > Regards > Gudrun Lang > > -----Urspr?ngliche Nachricht----- > Von: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Richard > Cartun > Gesendet: Freitag, 15. M?rz 2013 17:35 > An: Histonet > Betreff: [Histonet] PCR detection for Mycobacteria > > Is anyone offering clinical PCR detection for Mycobacteria from > formalin-fixed, paraffin-embedded tissue? Thanks! > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & Immunopathology > Director, Biospecimen Collection Programs Assistant Director, Anatomic > Pathology Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 545-1596 > (860) 545-2204 Fax > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 11 > Date: Sat, 16 Mar 2013 14:41:21 +0000 > From: "Troutman, Kenneth A" > Subject: RE: [Histonet] IHC validation > To: "Histonet@lists.utsouthwestern.edu" > > Cc: "'lcolbert@pathmdlabs.com'" > Message-ID: > > > Content-Type: text/plain; charset="us-ascii" > > Hi Laurie, > > Ideally, you would like to validate both with tissues processed in your laboratory (controls and patient tissue). Purchased TMAs are okay, but they should not be the only thing used. > > For the instrument validation, I would definitely use tissue that has been processed in your lab and run the same stain on each instrument (I think we ran serial sections of vimentin) and compare them very closely for any variation (use the same antibody and the same detection kit for each instrument). > > My recommendation for new antibody validations is to run a set of normal tissues and tumor tissues (we have about 15 normal tissues that we harvested and created our own sausage block) along with about 20 or so different tumors (also created in sausage blocks) to see how they stain under as many circumstances as possible (especially in the conditions your pathologists are most likely to see). > > In addition to that, run a set of known positives and known negatives from your cases. CAP recommends 10 of each, but I believe the final number is at the Medical Director's discretion. > > You can also run a representative sample of positives and negatives in your lab and send another set of the same tissues to another lab that has a validated procedure for that antibody and validate it that way. > > As far as the detection kit goes, we don't actually "validate" the detection kit per se. We do a lot-to-lot comparison, which involves running tissue from the same block (we created special blocks specifically for detection kits) and run the same antibody (AE1/AE3 or CD3--something we do a LOT of) to compare from lot to lot. > > I hope I answered your question. > > Regards, > > Ashley Troutman BS, HT(ASCP) QIHC > Immunohistochemistry Supervisor > Vanderbilt University Histopathology > 1301 Medical Center Drive TVC 4531 > Nashville, TN 37232 > ashley.troutman@vanderbilt.edu > > Message: 21 > Date: Fri, 15 Mar 2013 16:51:27 +0000 > From: Laurie Colbert > > Subject: [Histonet] IHC validation > To: "Histonet Post (histonet@lists.utsouthwestern.edu)" > > > Message-ID: > <12ECD7346266D74691EC2BFC75285E451CC048EB@BFL323E10.pathmdlabs.local> > Content-Type: text/plain; charset="us-ascii" > > If a lab is just starting up IHC for the first time and has to validate both the IHC stainer and the AB's/detection kit, does the validation have to be done using actual patient cases - or can you use strictly control tissue and purchased microarrays? > > Laurie Colbert, HT (ASCP) > > > > ------------------------------ > > Message: 12 > Date: Sat, 16 Mar 2013 08:39:06 -0700 > From: Kiranjit Grewal > Subject: [Histonet] Best Carmine stain > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: <8D2ECF37-6661-478D-886C-566493FDA66A@sbcglobal.net> > Content-Type: text/plain; charset=us-ascii > > Hello, > Any tips on doing Best Carmine on Frozen sections without water. Can we use mucicarmine solution to pick up glycogen in heart muscle? > Thank u, > Kiran > Sent from my iPhone > > > ------------------------------ > > Message: 13 > Date: Sat, 16 Mar 2013 09:07:08 -0700 (PDT) > From: Kim Donadio > Subject: Re: [Histonet] RE: IHC validation > To: "Morken, Timothy" , Laurie Colbert > , "Histonet Post > \(histonet@lists.utsouthwestern.edu\)" > > Message-ID: > <1363450028.62436.YahooMailNeo@web161601.mail.bf1.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > I agree about using known controls however sending cases out for comparison?could be a little costly, it is ideal though. One thing i have done is to go back in the records and pull up old cases that have been sent out for IHC and use those to compare not only the test as a case corrolation but also to compare any new controls I might want to continue using. Your pathologist should be able to verify accuracy and sign off on that.?? > ? > As far as the controls, Id purchase known controls those usually come with a stained example so you can compare that with your new controls and?any old known?cases you may have. Cases( blocks)?that are past thier?legal holding date of course.?? > ? > Good luck!! > ? > Kim D > ? > ? > ? > > > ________________________________ > From: "Morken, Timothy" > To: Laurie Colbert ; "Histonet Post (histonet@lists.utsouthwestern.edu)" > Sent: Friday, March 15, 2013 1:05 PM > Subject: [Histonet] RE: IHC validation > > Laurie, for first time validation use controls. Don't use patient cases that are done for initial diagnostics. If they are repeats to test the system, that is ok. > > The question is, who tested the controls? Ideally you will send a representative sample of your slides to another lab that uses the same or similar reagents to run for parallel testing. > > Tim Morken > Department of Pathology > UC San Francisco Medical Center > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert > Sent: Friday, March 15, 2013 9:51 AM > To: Histonet Post (histonet@lists.utsouthwestern.edu) > Subject: [Histonet] IHC validation > > If a lab is just starting up IHC for the first time and has to validate both the IHC stainer and the AB's/detection kit, does the validation have to be done using actual patient cases - or can you use strictly control tissue and purchased microarrays? > > Laurie Colbert, HT (ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 112, Issue 17 > ***************************************** From laka808 <@t> yahoo.com Sat Mar 16 12:45:45 2013 From: laka808 <@t> yahoo.com (Lisa) Date: Sat Mar 16 12:45:51 2013 Subject: [Histonet] Possible continuing automatic staining problem Message-ID: <91C5EFB9-F0A3-4CDB-B5F9-D26761EE6B70@yahoo.com> Hi We have this continuing problem, don't know if its a staining or possible processing problem, all our slides keep coming out dark blue & muted, we have adjusted the times every possible way, tried cutting thinner, we change our solutions regularly on our VIP processing machine. Our automatic strainer is a older tissue Tec but the Dr's have never been overjoyed with the stain, but lately even if we hand stain it, it still looks very muted under the microscope......Please any suggestions out there, Dr & histotech's very frustrated. Lisa A Sent from my iPad From jqb7 <@t> cdc.gov Sat Mar 16 13:43:53 2013 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/OID/NCEZID)) Date: Sat Mar 16 13:44:11 2013 Subject: [Histonet] Possible continuing automatic staining problem In-Reply-To: <91C5EFB9-F0A3-4CDB-B5F9-D26761EE6B70@yahoo.com> References: <91C5EFB9-F0A3-4CDB-B5F9-D26761EE6B70@yahoo.com> Message-ID: Are you talking bout H&E's? If so what hematoxylin and eosin are you using? What protocol? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lisa Sent: Saturday, March 16, 2013 1:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Possible continuing automatic staining problem Hi We have this continuing problem, don't know if its a staining or possible processing problem, all our slides keep coming out dark blue & muted, we have adjusted the times every possible way, tried cutting thinner, we change our solutions regularly on our VIP processing machine. Our automatic strainer is a older tissue Tec but the Dr's have never been overjoyed with the stain, but lately even if we hand stain it, it still looks very muted under the microscope......Please any suggestions out there, Dr & histotech's very frustrated. Lisa A Sent from my iPad _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ernestinemiddleton <@t> yahoo.ca Sun Mar 17 05:52:35 2013 From: ernestinemiddleton <@t> yahoo.ca (Ernestine Middleton) Date: Sun Mar 17 05:52:40 2013 Subject: [Histonet] No Subject Message-ID: <1363517555.65463.YahooMailNeo@web140704.mail.bf1.yahoo.com> http://www.nmksorgudbrandsdal.com/mlgthlu/bfwsvet?ghxab From asmith <@t> mail.barry.edu Sun Mar 17 11:32:08 2013 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Sun Mar 17 11:32:29 2013 Subject: [Histonet] Best Carmine stain In-Reply-To: <8D2ECF37-6661-478D-886C-566493FDA66A@sbcglobal.net> References: <8D2ECF37-6661-478D-886C-566493FDA66A@sbcglobal.net> Message-ID: Best's carmine and mucicarmine are very different stains. Mucicarmine is carminic acid mordanted with aluminum in acid solution. It stains mucus but not glycogen. Best's carmine is an ammoniated carminic acid, probably an amine at one end and an amide at the other. It is very basic. It is a selective stain for glycogen. It stains glycogen lightly, but the stain is permanent in xylene-soluble resin. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kiranjit Grewal Sent: Saturday, March 16, 2013 11:39 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Best Carmine stain Hello, Any tips on doing Best Carmine on Frozen sections without water. Can we use mucicarmine solution to pick up glycogen in heart muscle? Thank u, Kiran Sent from my iPhone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carl.hobbs <@t> kcl.ac.uk Sun Mar 17 12:55:59 2013 From: carl.hobbs <@t> kcl.ac.uk (Hobbs, Carl) Date: Sun Mar 17 12:56:09 2013 Subject: [Histonet] Thawing frozen tissue Message-ID: <00D6B8253EAED840B8D04E235497381822D7C383@AM2PRD0311MB399.eurprd03.prod.outlook.com> I slice away any excess OCT and immediately immerse in Formalin. Place on a rocker for 2 days. If you allow to thaw before fixation...you risk losing more proteins. Unlikely for many proteins but, not worth it, imho. Occam's Razor rules Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD School of Biomedical Sciences Kings College London Guys Campus SE1 1UL Tel: 020 78486813 Fax: 020 78486816 020 78486813 From w_alkadhumi <@t> yahoo.com Mon Mar 18 06:10:12 2013 From: w_alkadhumi <@t> yahoo.com (wassan alkadhumi) Date: Mon Mar 18 06:10:17 2013 Subject: [Histonet] link Message-ID: <1363605012.1049.YahooMailNeo@web121502.mail.ne1.yahoo.com> http://bystrzak.net/wgterx/liawzoic From akbitting <@t> geisinger.edu Mon Mar 18 07:49:23 2013 From: akbitting <@t> geisinger.edu (Bitting, Angela K.) Date: Mon Mar 18 07:49:34 2013 Subject: [Histonet] Billing for Pin 4 Cocktail In-Reply-To: References: <1363363219.67860.YahooMailClassic@web161202.mail.bf1.yahoo.com> <5143014D020000230004BE7C@gwgwia1.luhs.org> Message-ID: <77F52EFAB8B1694B885E277C48FCD0F639B53434@GHSEXMBX4W8K1V.geisinger.edu> I was given info from a Dako rep last week that if the primary antibodies are "applied" separately to the slide as is true with Ventana instruments, you can charge for both. I am skeptical. Has anyone else heard this? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vanessa Perez Sent: Friday, March 15, 2013 12:43 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Billing for Pin 4 Cocktail Its actually not allowed already. Any cocktail where all stains are done at one time on one slide can only be charged x1 even if its 2 or more antibodys in the cocktail. One of our reps updated us with our PIN cocktail -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Roger Heyna Sent: Friday, March 15, 2013 11:09 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Billing for Pin 4 Cocktail Our lab currently bills for three, and I know of other labs in our area that also bill for three. I have heard rumors that this may not be allowed in the near future. Roger Heyna Maywood, IL >>> Debbie Granato 3/15/2013 11:00 AM >>> We have a billing question for the PIN4 Cocktail that we perform on prostate needle biopsies. We currently use the Biocare CK5+CK14+p63 and then add the Biocare P 504S in the lab. How would you bill for this? Would billing for 1 stain be correct or can you bill for 3 stains? Thank you for your input. Debbie Granato _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. ------------------------------------------------------------------------- This message was secured by ZixCorp(R). From MICHELLE.LAMPHERE <@t> childrens.com Mon Mar 18 08:00:51 2013 From: MICHELLE.LAMPHERE <@t> childrens.com (Michelle Lamphere) Date: Mon Mar 18 08:03:26 2013 Subject: [Histonet] Equipment service Message-ID: Does anybody in the DFW area have a vendor or company (other than the manufacturer) that they would recommend to service their histology equipment? Annual PMs, emergency repairs, etc... Michelle M Lamphere, HT (ASCP) Senior Tech, Histology Children's Medical Center 1935 Medical District Drive Dallas, TX 75235 Office :214-456-2798 Histology: 214-456-2318 Fax: 214-456-0779 Please consider the environment before printing this e-mail This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. Please consider the environment before printing this e-mail. This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. From MICHELLE.LAMPHERE <@t> childrens.com Mon Mar 18 08:14:04 2013 From: MICHELLE.LAMPHERE <@t> childrens.com (Michelle Lamphere) Date: Mon Mar 18 08:14:59 2013 Subject: [Histonet] Tracking OR specimens Message-ID: Are there any histology labs that actively participate in auditing the Operating Room on a daily basis to make sure that histology receives all of the specimens that the OR should have submitted? If so, how do you do this? Or should the OR be solely responsible for making sure that they specimens make it to histology? Michelle M Lamphere, HT (ASCP) Senior Tech, Histology Children's Medical Center 1935 Medical District Drive Dallas, TX 75235 Office :214-456-2798 Histology: 214-456-2318 Fax: 214-456-0779 Please consider the environment before printing this e-mail This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. Please consider the environment before printing this e-mail. This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. From ratliffjack <@t> hotmail.com Mon Mar 18 08:17:03 2013 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Mon Mar 18 08:17:10 2013 Subject: [Histonet] Bone processing help In-Reply-To: References: <0F62C13F3FBAE541AC2D6AE56E26E85041D30F@PHSX10MB15.partners.org> Message-ID: Dr. De la Vega, My name is Jack Ratliff and I Chair the Hard Tissue Committee for the National Society for Histotechnology. I am not sure if anyone has provided you with a response to your message, but maybe I can be of assistance. Just so you know, the NSH prides itself on being an educational resource for those working in the field of Histology. The Hard Tissue Committee represents the educational arm of the society for those working with "hard tissues" like bone, biomaterials and medical device implants and especially those type of histological specimens embedded into resins or plastics! I would like to first direct your attention to the NSH website at www.nsh.org. If you go to the following link: http://www.nsh.org/content/benefits-membership, you will find information concerning membership benefits and how to join so that you can take advantage of what the society can do for you and your laboratory! Please visit this site first and then feel free to get back to me with any questions. As for your current issues, I can definitely help you with all that you are experiencing. At the moment I am currently away from the US and in Germany until the 26th of March working with a group (Rowiak GmbH - www.rowiak.de) that has developed a non-contact laser microtome (Tissue Surgeon) that can do what you are doing now and produce stained slides @ 10-15 microns in 30 minutes from a resin/plastic embedded block! If you can give me a few more hours, I will personally respond to your message privately. In the meantime, please visit the NSH website (www.nsh.org), consider its membership and also look to join the Hard Tissue Committee so that I can provide you with meeting updates. With that said and seeing that you are in Massachusetts, on May the 4th, 2013, Polysciences, Inc. is hosting a full day educational event focusing on the *Histological Applications & Techniques for Bone, Biomaterials and Medical Device Implants*. I will also be a speaker at this event. Those that attend can expect to further their knowledge, understanding and training of specialized histology techniques associated with bone, biomaterials and medical device implant specimen types and attendees will learn of the applicational relevance of the techniques used in the evaluation safety and efficacy of therapeutic treatments. Registration is currently open with an *Early Bird registration set to end this Friday, March 22nd*. The National Society for Histotechnology (NSH) will be providing 6 CEUs and Polysciences, Inc. will be providing a discount to any current NSH member as outlined below: *WORKSHOP FEES* * * *Before March 22, 2013: $149.00 for NSH Members / $199.00 for Non-Members* * * *After March 22, 2013: $179.00 for NSH Members / $229.00 for Non-Members* For the complete details of this full day *Histological Applications & Techniques for Bone, Biomaterials and Medical Device Implants *event and to register online, please visit the following link: * http://www.polysciences.com/Interactive-Histology-Forum-About/185/* and sign up today to participate in the discussion of these specialized histology specimen applications and techniques that are rarely shared or even discussed on Histonet! You will not want to miss out on the information presented by 4 expert speakers in the field, all course materials, meals and a complete program book also containing technique specific protocols that you can repeat back in your lab! Best Regards, Jack Jack L. Ratliff Owner/Histologist, Ratliff Histology Consultants, LLC Chairman, Hard Tissue Committee - National Society for Histotechnology 389 Nichol Mill Lane Franklin, TN 37067 (317) 281-1975 (c) (615) 236-4901 (o) (615) 236-4962 (f) jratliff@ratliffhistology.com On Sun, Mar 17, 2013 at 8:09 PM, Jack Ratliff wrote: > > > > Begin forwarded message: > > *From:* "De La Vega Amador, Rodolfo Enrique" > > *Date:* March 15, 2013, 9:41:23 PM GMT+01:00 > *To:* "histonet@lists.utsouthwestern.edu" < > histonet@lists.utsouthwestern.edu> > *Subject:* *[Histonet] Bone processing help* > > Hi everybody, > > I am fairly new to all Histotechnology processes. We mainly work in our > lab with mineralized bone with implants, so we fix them, dehydrate them, > embed them in MMA, cut them, glued them to slides, ground them manually and > then stain them. I need help in some parts of the process that need > improvement: > > > 1. We use Loctite 4471 on the samples, put them under vacuum, apply them > to the slide and more vacuum. There's good results but there are some > bubbles that still appear. Suggestions? > 2. I can't seem to get the yellow/orange on bone with the Van Gieson > stain. I've been doinga preheat at 55 ?C, etching, rinse in DI water, > Sanderson?s Rapid Bone Stain, running tap water, Van Gieson (commercial > from DHM) for 30 seconds to 5 minutes, paper dry and quickly dehydrate with > 100% EtOH. What am I doing wrong? > 3. The stain's been running when I add the glue to cover slip the > slides. Especially the green color. Why does this happen? > 4. Cover slipping is done with cyano acrylate, under suction as before, > but bubbles still appear. I've tried placing the cover slip on an angle and > wait for the glue to evenly spread, but the bone has just too many pores > and air bubbles appear. Please help. > > I greatly appreciate any help on the subject. > > Best, > > Rodolfo De la Vega, MD. > Research Fellow > Laboratory for Musculoskeletal Research and Innovation > Massachusetts General Hospital - Jackson Building 1120 > > > > The information in this e-mail is intended only for the person to whom it > is > addressed. If you believe this e-mail was sent to you in error and the > e-mail > contains patient information, please contact the Partners Compliance > HelpLine at > http://www.partners.org/complianceline . If the e-mail was sent to you in > error > but does not contain patient information, please contact the sender and > properly > dispose of the e-mail. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From trathborne <@t> somerset-healthcare.com Mon Mar 18 08:21:16 2013 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Mon Mar 18 08:21:21 2013 Subject: [Histonet] RE: Tracking OR specimens In-Reply-To: References: Message-ID: <3AD061FE740D464FAC7BF6B5CFB757076A296B6B@smcmail02.somerset-healthcare.com> We are able to view a pending log. Once a patient is in the OR, nurses will place an order for the specimen. If we have not received the specimen in the anticipated time, we will call. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michelle Lamphere Sent: Monday, March 18, 2013 9:14 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Tracking OR specimens Are there any histology labs that actively participate in auditing the Operating Room on a daily basis to make sure that histology receives all of the specimens that the OR should have submitted? If so, how do you do this? Or should the OR be solely responsible for making sure that they specimens make it to histology? Michelle M Lamphere, HT (ASCP) Senior Tech, Histology Children's Medical Center 1935 Medical District Drive Dallas, TX 75235 Office :214-456-2798 Histology: 214-456-2318 Fax: 214-456-0779 Please consider the environment before printing this e-mail This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. Please consider the environment before printing this e-mail. This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek <@t> digestivespecialists.com Mon Mar 18 08:21:52 2013 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Mon Mar 18 08:24:44 2013 Subject: [Histonet] RE: Tracking OR specimens In-Reply-To: References: Message-ID: <5A2BD13465E061429D6455C8D6B40E39164D4BF602@IBMB7Exchange.digestivespecialists.com> Michelle, We have a check sheet that has all cases scheduled for the day. There is a place to log how many specimens were collected for each case. If there were no specimens collected then that is logged also. Linda Linda Blazek HT (ASCP) lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michelle Lamphere Sent: Monday, March 18, 2013 9:14 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Tracking OR specimens Are there any histology labs that actively participate in auditing the Operating Room on a daily basis to make sure that histology receives all of the specimens that the OR should have submitted? If so, how do you do this? Or should the OR be solely responsible for making sure that they specimens make it to histology? Michelle M Lamphere, HT (ASCP) Senior Tech, Histology Children's Medical Center 1935 Medical District Drive Dallas, TX 75235 Office :214-456-2798 Histology: 214-456-2318 Fax: 214-456-0779 Please consider the environment before printing this e-mail This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. Please consider the environment before printing this e-mail. This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BZIMMERM <@t> gru.edu Mon Mar 18 08:28:04 2013 From: BZIMMERM <@t> gru.edu (Zimmerman, Billie) Date: Mon Mar 18 08:33:28 2013 Subject: [Histonet] Georgia Society for Histology---IT'S NOT TOO LATE!!! What cha' gonna do at Jekyll?? Message-ID: <7B3DEB32E69C034EACB479059C5DE3FF75C0B7@EX-MLB-03.ad.georgiahealth.edu> Room rates remain at the symposium price IF AVAILABLE. (all inclusive price is $135) If anyone happens to say they are bored at the GSH meeting...I just wouldn't understand. Here's a mini list : * Sunrises and Sunsets (personally I've never seen a sunrise at the beach, what's the point, you can see the same thing on the other side in the evening, I'm not a morning person but maybe I will put a sunrise on my bucket list.) Don't tell Wanda I said this! * Turtle center * Kayaking and fishing * Horseback riding on the beach * Bicycle trails * Historic golf course * Tennis center * Historic Jekyll island club and village * High Tea at the Jekyll Club * Dolphin tours * Karaoke in the Sandbar Lounge * Putt putt golf * Fresh seafood * Brunswick stew( originated in the area) Original version had squirrel and/or rabbit EEEWWWEEE!!! Augusta State University and Georgia Health Sciences University have consolidated to become Georgia Regents University. Effective January 9, 2013, my email address has changed to BZIMMERM@gru.edu. Please update your address book to reflect this change. From Nancy_Schmitt <@t> pa-ucl.com Mon Mar 18 08:47:14 2013 From: Nancy_Schmitt <@t> pa-ucl.com (Nancy Schmitt) Date: Mon Mar 18 08:47:24 2013 Subject: [Histonet] yeast comtamination on surepath Message-ID: <906B4DA90ED1DB4DB6C7E94D7CEE6C364DC73505@PEITHA.wad.pa-ucl.com> Happy Monday! We are running surepath paps on the prepstain instrument. We are seeing yeast - and it seems to occur after we have had a PM done. I am wondering if anyone else sees this and how you are handling it. We have been bleaching the lines each day the cytotechs report they have seen it, but it seems to keep popping up. We filter our stains and perform routine maintenance as instructed. I have contacted technical support - they were not so helpful:( I will be giving that another whirl if doesn't clear up soon. Thank you for any advice - and I know this is a histology site, but I have been impressed with the cytoprep advice I have received here. Nancy Schmitt HT, MLT(ASCP) Histology Coordinator Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From TMcNemar <@t> lmhealth.org Mon Mar 18 09:38:01 2013 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Mon Mar 18 09:37:11 2013 Subject: [Histonet] RE: Tracking OR specimens In-Reply-To: References: Message-ID: Our OR uses a binder with log sheets. They write the specimen source one of the left over patient labels and place it onto the log sheet. We then match them up with the actual specimens received and initial the label. It works very well since any discrepancies are cleared up before the specimens ever leave the OR. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michelle Lamphere Sent: Monday, March 18, 2013 9:14 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Tracking OR specimens Are there any histology labs that actively participate in auditing the Operating Room on a daily basis to make sure that histology receives all of the specimens that the OR should have submitted? If so, how do you do this? Or should the OR be solely responsible for making sure that they specimens make it to histology? Michelle M Lamphere, HT (ASCP) Senior Tech, Histology Children's Medical Center 1935 Medical District Drive Dallas, TX 75235 Office :214-456-2798 Histology: 214-456-2318 Fax: 214-456-0779 Please consider the environment before printing this e-mail This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. Please consider the environment before printing this e-mail. This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From DKBoyd <@t> chs.net Mon Mar 18 10:13:13 2013 From: DKBoyd <@t> chs.net (Boyd, Debbie M) Date: Mon Mar 18 10:13:28 2013 Subject: [Histonet] RE: Tracking OR specimens In-Reply-To: References: Message-ID: <7EAFE982E328304DA6CE2B677BB76246794C9EE4@TN001WEXMBX12.US.chs.net> Yes. We have the OR send us there Operating Room Log, daily. Of course we get it the next day. It is what is logged into the computer system. Not just a label on a log sheet (we do that also). I reconcile the OR Log daily. We have actually caught specimens that were being tossed in the trash even though they were not on the exempt list. We have also found specimens that were left in a room or under a gurney during transport. Unfortunately, one was never recovered, but we could notify the physician immediately, instead of a couple weeks, when the patient is setting in his office. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michelle Lamphere Sent: Monday, March 18, 2013 9:14 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Tracking OR specimens Are there any histology labs that actively participate in auditing the Operating Room on a daily basis to make sure that histology receives all of the specimens that the OR should have submitted? If so, how do you do this? Or should the OR be solely responsible for making sure that they specimens make it to histology? Michelle M Lamphere, HT (ASCP) Senior Tech, Histology Children's Medical Center 1935 Medical District Drive Dallas, TX 75235 Office :214-456-2798 Histology: 214-456-2318 Fax: 214-456-0779 Please consider the environment before printing this e-mail This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. Please consider the environment before printing this e-mail. This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From wdesalvo.cac <@t> outlook.com Mon Mar 18 10:24:56 2013 From: wdesalvo.cac <@t> outlook.com (WILLIAM DESALVO) Date: Mon Mar 18 10:25:02 2013 Subject: [Histonet] Tracking OR specimens In-Reply-To: References: Message-ID: We use the OR schedule and have a log at Surgical Pathology that the person delivering specimens places a patient label w/ the number of specimens delivered and they sign off. This has helped many times when OR believes they have delivered all the specimens. We use both the log and OR schedule to reconcile cases and contact OR is a case has not been delivered by end of day. William DeSalvo, BS HTL(ASCP) Production Manager-Anatomic Pathology Chair, NSH Quality Management Committee Owner/Consultant, Collaborative Advantage Consulting > From: MICHELLE.LAMPHERE@childrens.com > To: histonet@lists.utsouthwestern.edu > Date: Mon, 18 Mar 2013 13:14:04 +0000 > Subject: [Histonet] Tracking OR specimens > > Are there any histology labs that actively participate in auditing the Operating Room on a daily basis to make sure that histology receives all of the specimens that the OR should have submitted? If so, how do you do this? Or should the OR be solely responsible for making sure that they specimens make it to histology? > > Michelle M Lamphere, HT (ASCP) > Senior Tech, Histology > Children's Medical Center > 1935 Medical District Drive > Dallas, TX 75235 > Office :214-456-2798 > Histology: 214-456-2318 > Fax: 214-456-0779 > > > Please consider the environment before printing this e-mail > > This e-mail, facsimile, or letter and any files or attachments transmitted with it contains > information that is confidential and privileged. This information is intended only for the use of the > individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further > disclosures are prohibited without proper authorization. If you are not the intended recipient, any > disclosure, copying, printing, or use of this information is strictly prohibited and possibly a > violation of federal or state law and regulations. If you have received this information in error, > please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at > privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all > applicable privileges related to this information. > > Please consider the environment before printing this e-mail. > > This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 <@t> earthlink.net Mon Mar 18 11:18:12 2013 From: relia1 <@t> earthlink.net (Pam Barker) Date: Mon Mar 18 12:59:26 2013 Subject: [Histonet] RELIA Histology Careers Bulletin Hot Jobs in NY, CA, GA, NC, PA, KY and OH March Madness is here!!! Message-ID: <007a01ce23f4$305bad70$91130850$@earthlink.net> Hi Histonetters! I Hope You Had A Fun And Lucky St. Patricks Day. And a Happy Histotechnology Professionals Day. (Did your lab do anything special?) March Madness has started and in addition to some great College Basketball. I have been experiencing the madness as well. My phone has literally been ringing off the hook with some great new opportunities. All of these positions are permanent full time positions and my clients offer excellent compensation, benefits and relocation assistance. Best of all these clients are motivated to hire and eager to meet you! Here is a list of my current openings: HISTOLOGY/PATHOLOGY MANAGEMENT Vancouver, WA ? Molecular Diagnostics Supervisor (multi lab responsibilities) Atlanta, GA ? Technical Coordinator ? Pathology Atlanta, GA ? Lead Histotechnologist San Francisco, CA ? Pathology Lab Manager ? previous academic exper req. Long Island, NY - Hematopathologist HISTOTECHS NC ? Charlotte Grossing Histotech NC ? Charlotte, IHC Specialist NC ? Charlotte ? Histology Tech PA ? Philadelphia ? FISH Tech NY ? NYC ? IHC Specialist NY ? Long Island FISH, FLOWand histology openings NY ? Syracuse ? Histology Tech NY Lic or eligible is required KY ? Louisville ? HT/HTL GA ? Atlanta ? HT/HTL OH ? Zanesville ? HT/HTL CA ? Long Beach ? Mohs Histotech SPECIAL JOB ALERT!! A client in NY has asked for help with their recruitment of a Hematopathologist. Do you know anyone for this position? If you refer someone that I place in this position I will double referral fee to $1000.00 This includes confidential referrals. Meaning if you give me the name of a hematopathologist and I contact them confidentially and place them you also get the referral fee. In other words you don?t have to introduce me to them. Just tell me who to call! If you or anyone you know might be interested in any of these positions or want help with a job search in another area please contact me. I can be reached at 866-607-3542 or relia1@earthlink.net. Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker ? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From tkngflght <@t> yahoo.com Mon Mar 18 11:45:48 2013 From: tkngflght <@t> yahoo.com (Cheryl) Date: Mon Mar 18 13:09:30 2013 Subject: [Histonet] Histo jobs: one temp - one permanent Message-ID: <1363625148.7799.YahooMailClassic@web161903.mail.bf1.yahoo.com> Hi All- ? Looking for a senior skilled tech for a day shift temp in the Northeast....email for more specifics. ? Also seeking a solid routine tech for a permanent position at my home lab in Louisiana....great people, good equipment, nice place to call home. ? As always--email or phone for more information and feel free to pass along to a friend! ? Cheryl Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab by helping one GREAT?Tech at a time.? 281.852.9457?Office 800.756.3309?Phone & Fax? admin@fullstaff.org Sign up for the FREE?newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please?include your name and specialty in the body of the email. From rheyna <@t> lumc.edu Mon Mar 18 12:11:15 2013 From: rheyna <@t> lumc.edu (Roger Heyna) Date: Mon Mar 18 13:13:48 2013 Subject: [Histonet] Billing for Pin 4 Cocktail In-Reply-To: <77F52EFAB8B1694B885E277C48FCD0F639B53434@GHSEXMBX4W8K1V.geisinger.edu> References: <1363363219.67860.YahooMailClassic@web161202.mail.bf1.yahoo.com> <5143014D020000230004BE7C@gwgwia1.luhs.org> <77F52EFAB8B1694B885E277C48FCD0F639B53434@GHSEXMBX4W8K1V.geisinger.edu> Message-ID: <51470463020000230004C056@gwgwia1.luhs.org> Our billing manager said the same thing. If the antibodies are being applied separately, you can bill for each one. If antibodies are being mixed and applied at the same time, you can only bill for one. It sounds like the difference between a double-stain (two different chromogens) and a single stain using an antibody cocktail. I still don't think we're doing it correctly, because we're billing for three, but at least it sounds like we can bill for two, which seems logical. Have others heard something different? Roger Heyna Maywood, IL >>> "Bitting, Angela K." 3/18/2013 7:49 AM >>> I was given info from a Dako rep last week that if the primary antibodies are "applied" separately to the slide as is true with Ventana instruments, you can charge for both. I am skeptical. Has anyone else heard this? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vanessa Perez Sent: Friday, March 15, 2013 12:43 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Billing for Pin 4 Cocktail Its actually not allowed already. Any cocktail where all stains are done at one time on one slide can only be charged x1 even if its 2 or more antibodys in the cocktail. One of our reps updated us with our PIN cocktail -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Roger Heyna Sent: Friday, March 15, 2013 11:09 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Billing for Pin 4 Cocktail Our lab currently bills for three, and I know of other labs in our area that also bill for three. I have heard rumors that this may not be allowed in the near future. Roger Heyna Maywood, IL >>> Debbie Granato 3/15/2013 11:00 AM >>> We have a billing question for the PIN4 Cocktail that we perform on prostate needle biopsies. We currently use the Biocare CK5+CK14+p63 and then add the Biocare P 504S in the lab. How would you bill for this? Would billing for 1 stain be correct or can you bill for 3 stains? Thank you for your input. Debbie Granato _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. ------------------------------------------------------------------------- This message was secured by ZixCorp(R). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Erin.Martin <@t> ucsf.edu Mon Mar 18 13:28:13 2013 From: Erin.Martin <@t> ucsf.edu (Martin, Erin) Date: Mon Mar 18 13:28:28 2013 Subject: [Histonet] Immunofluorescence on FFPE skin Message-ID: <24B7B291CC88D04AB663958E77A1F59D1567D4@ex09.net.ucsf.edu> Hello all, My pathologist gave me a copy of "Immunofluorescence with Dual Microwave Retrieval of Paraffin-Embedded Sections in the Assessment of Human Renal Biopsy Specimens" from the American Journal of Clinical Pathology. He said that the same principle should work on skin and he would like to be able to do IF on fixed tissue in addition to our usual cryostat sections. Has anyone else read the paper who might be willing to give me some basic advice to try working it out? Is a microwave necessary (paper's method uses 2 different wattage settings) or is there a way to use HIER in waterbath or pressure cooker? Thanks in advance, Erin Erin Martin, Histology Supervisor UCSF Dermatopathology Service 415-353-7248 Confidentiality Notice The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential and/or priviledged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited. If you receive this in error, please contact the sender and delete the material from any computer. From PAMarcum <@t> uams.edu Mon Mar 18 13:58:52 2013 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Mon Mar 18 13:59:02 2013 Subject: [Histonet] Does anyone sell or have Bielchowsky slides for sale? Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA32973A6AC9@Mail2Node2.ad.uams.edu> Please let me know if you can help on this one. Pam Marcum UAMS Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From PAMarcum <@t> uams.edu Mon Mar 18 14:38:50 2013 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Mon Mar 18 14:38:55 2013 Subject: [Histonet] RE: Does anyone sell or have Bielchowsky slides for sale? In-Reply-To: <62C639732D3F274DACED033EBDF6ADAF20D11006@evcspmbx2.ads.northwestern.edu> References: <41D3A1AF6FEF0643BDC89E0516A6EA32973A6AC9@Mail2Node2.ad.uams.edu> <62C639732D3F274DACED033EBDF6ADAF20D11006@evcspmbx2.ads.northwestern.edu> Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA32973A6ADF@Mail2Node2.ad.uams.edu> Sorry, I was not clear. I need the control slides if someone offers them. -----Original Message----- From: Bernice Frederick [mailto:b-frederick@northwestern.edu] Sent: Monday, March 18, 2013 2:15 PM To: Marcum, Pamela A Subject: RE: Does anyone sell or have Bielchowsky slides for sale? Are you looking for control slides? I see Newcomer and Rowley have the staining kits. Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marcum, Pamela A Sent: Monday, March 18, 2013 1:59 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Does anyone sell or have Bielchowsky slides for sale? Please let me know if you can help on this one. Pam Marcum UAMS Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From Wanda.Smith <@t> HCAhealthcare.com Mon Mar 18 15:00:03 2013 From: Wanda.Smith <@t> HCAhealthcare.com (Wanda.Smith@HCAhealthcare.com) Date: Mon Mar 18 15:03:36 2013 Subject: [Histonet] RE: Tracking OR specimens In-Reply-To: References: Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BA27D512B430@NADCWPMSGCMS03.hca.corpad.net> Good Afternoon, Our OR furnishes a specimen log that their personnel fills out with date, time, patient label, lists of all specimens, dr, their initials and Pathology personnel signs off when they get the specimen out of the window. If there are any problems or issues, we call the OR and meet someone from the case in the window to resolve the issue. I think it is the OR's responsibility to get the specimens from the OR to the window. Thanks, Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC? 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michelle Lamphere Sent: Monday, March 18, 2013 9:14 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Tracking OR specimens Are there any histology labs that actively participate in auditing the Operating Room on a daily basis to make sure that histology receives all of the specimens that the OR should have submitted? If so, how do you do this? Or should the OR be solely responsible for making sure that they specimens make it to histology? Michelle M Lamphere, HT (ASCP) Senior Tech, Histology Children's Medical Center 1935 Medical District Drive Dallas, TX 75235 Office :214-456-2798 Histology: 214-456-2318 Fax: 214-456-0779 Please consider the environment before printing this e-mail This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. Please consider the environment before printing this e-mail. This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From allison-malandra <@t> uiowa.edu Mon Mar 18 15:24:22 2013 From: allison-malandra <@t> uiowa.edu (Malandra, Allison E) Date: Mon Mar 18 15:25:25 2013 Subject: [Histonet] Fluorochrome bone labeling in dogs Message-ID: <451035461C585B4BAEBFC8ADAC7F804A2403E9A9@HC-MAILBOXC1-N5.healthcare.uiowa.edu> Good afternoon - Our lab is planning an upcoming study that involves using fluorochrome bone labels in hound dogs. One is oxytetracycline at 30 mg/kg IV. Back when I was a young veterinarian, I took care of two dogs that went into acute renal failure after they were given that dose. I know that oxytet is widely used as a bone label in dogs but the nephrotoxicosis has been reported in several citations. A PubMed search revealed a wide range of dosing of oxytet for bone labeling in dogs (usually between 10-30 mg/kg) used IV, SQ or IM. Can anyone share their oxytetracycline labeling protocol for dogs? We would prefer to not give it IV if possible but I have heard of granulomas forming IM. Has anyone experienced the renal toxic effects? I believe one paper looked at giving parenteral fluids at time of administration to help with diuresis but I am awaiting inter-library loan delivery for the full citation. I appreciate everyone's time and help. Allison Malandra, DVM Bone Healing Research Lab University of Iowa ________________________________ Notice: This UI Health Care e-mail (including attachments) is covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, is confidential and may be legally privileged. If you are not the intended recipient, you are hereby notified that any retention, dissemination, distribution, or copying of this communication is strictly prohibited. Please reply to the sender that you have received the message in error, then delete it. Thank you. ________________________________ From tony.henwood <@t> health.nsw.gov.au Mon Mar 18 17:32:50 2013 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Mon Mar 18 17:33:55 2013 Subject: [Histonet] RE: yeast comtamination on surepath In-Reply-To: <906B4DA90ED1DB4DB6C7E94D7CEE6C364DC73505@PEITHA.wad.pa-ucl.com> References: <906B4DA90ED1DB4DB6C7E94D7CEE6C364DC73505@PEITHA.wad.pa-ucl.com> Message-ID: <6D6BD1DE8A5571489398B392A38A71579D237E18@xmdb04.nch.kids> Nancy, The yeast may be growing in the Specimen collection vials. I found a similar problem with environmental yeasts growing in Hanks media (Henwood, A. (2011), Fungal contamination of Hanks solution. Diagn. Cytopathol. doi: 10.1002/dc.21798) I would recommend random cytocentrifuge preps, air-dried and stained with Giemsa or DiffQuik, taken from different lots of the un-used collection vials as a QC. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt Sent: Tuesday, 19 March 2013 12:47 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] yeast comtamination on surepath Happy Monday! We are running surepath paps on the prepstain instrument. We are seeing yeast - and it seems to occur after we have had a PM done. I am wondering if anyone else sees this and how you are handling it. We have been bleaching the lines each day the cytotechs report they have seen it, but it seems to keep popping up. We filter our stains and perform routine maintenance as instructed. I have contacted technical support - they were not so helpful:( I will be giving that another whirl if doesn't clear up soon. Thank you for any advice - and I know this is a histology site, but I have been impressed with the cytoprep advice I have received here. Nancy Schmitt HT, MLT(ASCP) Histology Coordinator Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From mohit.koladia <@t> ndsu.edu Tue Mar 19 01:20:43 2013 From: mohit.koladia <@t> ndsu.edu (Mohit Koladia) Date: Tue Mar 19 01:20:52 2013 Subject: [Histonet] Thionin / Acid fuchsin staining Rat brain Message-ID: *Hello Histonetters,* * * *I am looking for the staining protocol for thionin/Acid fucshin staining.* * * *I have Rat brain slides which are paraffin embedded and sectioned at 5-7 um. * *I have had a problem to make a Acid fucshin solution as it does not stick to the brain slices.* *Please give proper recipe for the same.* *I tried to search a lot but i didnt find any good article or literature that helps figuring this out.* * * *Would appreciate if someone can help me in this regards.* * * *Thanks,* * Mohit Koladia (B.Pharm, MS Pharm) Graduate Research Scholar, North Dakota State University, Fargo, ND, USA-58102* From rudymateo2000 <@t> yahoo.com Tue Mar 19 01:21:00 2013 From: rudymateo2000 <@t> yahoo.com (Rudy Mateo) Date: Tue Mar 19 01:21:08 2013 Subject: [Histonet] service manual Hacker Mesei 3655 Robotic Coverslipper Message-ID: <1363674060.88360.YahooMailClassic@web163905.mail.gq1.yahoo.com> We were given a Hacker Mesei 3655 Coverslipper as donation for our research. Unfortunately it falters during operation. We badly need a Service Manual for it to go up and running again. Could ?you possibly provide us ?even a PDF copy of service manual for this item?Kindly help us by offering a proposal.Thank you.R. Mateoat rudymateo2000@yahoo.com From Melissa.Kuhnla <@t> chsli.org Tue Mar 19 06:10:33 2013 From: Melissa.Kuhnla <@t> chsli.org (Kuhnla, Melissa) Date: Tue Mar 19 06:11:42 2013 Subject: [Histonet] Billing for Pin 4 Cocktail In-Reply-To: <51470463020000230004C056@gwgwia1.luhs.org> References: <1363363219.67860.YahooMailClassic@web161202.mail.bf1.yahoo.com><5143014D020000230004BE7C@gwgwia1.luhs.org><77F52EFAB8B1694B885E277C48FCD0F639B53434@GHSEXMBX4W8K1V.geisinger.edu> <51470463020000230004C056@gwgwia1.luhs.org> Message-ID: I always thought you could bill for three due to three distinguishable staining patterns. The two cytokeratins can not be distinguished from each other, therefore three charges instead of four (PIN4). -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Roger Heyna Sent: Monday, March 18, 2013 1:11 PM To: Angela K. Bitting; histonet@lists.utsouthwestern.edu; Vanessa Perez Subject: RE: [Histonet] Billing for Pin 4 Cocktail Our billing manager said the same thing. If the antibodies are being applied separately, you can bill for each one. If antibodies are being mixed and applied at the same time, you can only bill for one. It sounds like the difference between a double-stain (two different chromogens) and a single stain using an antibody cocktail. I still don't think we're doing it correctly, because we're billing for three, but at least it sounds like we can bill for two, which seems logical. Have others heard something different? Roger Heyna Maywood, IL >>> "Bitting, Angela K." 3/18/2013 7:49 AM >>> I was given info from a Dako rep last week that if the primary antibodies are "applied" separately to the slide as is true with Ventana instruments, you can charge for both. I am skeptical. Has anyone else heard this? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vanessa Perez Sent: Friday, March 15, 2013 12:43 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Billing for Pin 4 Cocktail Its actually not allowed already. Any cocktail where all stains are done at one time on one slide can only be charged x1 even if its 2 or more antibodys in the cocktail. One of our reps updated us with our PIN cocktail -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Roger Heyna Sent: Friday, March 15, 2013 11:09 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Billing for Pin 4 Cocktail Our lab currently bills for three, and I know of other labs in our area that also bill for three. I have heard rumors that this may not be allowed in the near future. Roger Heyna Maywood, IL >>> Debbie Granato 3/15/2013 11:00 AM >>> We have a billing question for the PIN4 Cocktail that we perform on prostate needle biopsies. We currently use the Biocare CK5+CK14+p63 and then add the Biocare P 504S in the lab. How would you bill for this? Would billing for 1 stain be correct or can you bill for 3 stains? Thank you for your input. Debbie Granato _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. ------------------------------------------------------------------------ - This message was secured by ZixCorp(R). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. From SStephenson <@t> lifecell.com Tue Mar 19 06:24:05 2013 From: SStephenson <@t> lifecell.com (Stephenson, Sheryl) Date: Tue Mar 19 06:26:58 2013 Subject: [Histonet] Possible continuing automatic staining problem In-Reply-To: <91C5EFB9-F0A3-4CDB-B5F9-D26761EE6B70@yahoo.com> References: <91C5EFB9-F0A3-4CDB-B5F9-D26761EE6B70@yahoo.com> Message-ID: What do you mean by "muted"; can you give a little more description? And what type of tissue, and is this H&E? Sheryl Stephenson | Histology Technician -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lisa Sent: Saturday, March 16, 2013 1:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Possible continuing automatic staining problem Hi We have this continuing problem, don't know if its a staining or possible processing problem, all our slides keep coming out dark blue & muted, we have adjusted the times every possible way, tried cutting thinner, we change our solutions regularly on our VIP processing machine. Our automatic strainer is a older tissue Tec but the Dr's have never been overjoyed with the stain, but lately even if we hand stain it, it still looks very muted under the microscope......Please any suggestions out there, Dr & histotech's very frustrated. Lisa A Sent from my iPad _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vperez <@t> pathreflab.com Tue Mar 19 07:39:52 2013 From: vperez <@t> pathreflab.com (Vanessa Perez) Date: Tue Mar 19 07:40:42 2013 Subject: [Histonet] Billing for Pin 4 Cocktail In-Reply-To: <77F52EFAB8B1694B885E277C48FCD0F639B53434@GHSEXMBX4W8K1V.geisinger.edu> References: <1363363219.67860.YahooMailClassic@web161202.mail.bf1.yahoo.com> <5143014D020000230004BE7C@gwgwia1.luhs.org> <77F52EFAB8B1694B885E277C48FCD0F639B53434@GHSEXMBX4W8K1V.geisinger.edu> Message-ID: Well we use a cocktail, so the antibodies are mixed together and so applied at the same time. All in the same dispenser not two diff one. Vanessa -----Original Message----- From: Bitting, Angela K. [mailto:akbitting@geisinger.edu] Sent: Monday, March 18, 2013 7:49 AM To: Vanessa Perez; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Billing for Pin 4 Cocktail I was given info from a Dako rep last week that if the primary antibodies are "applied" separately to the slide as is true with Ventana instruments, you can charge for both. I am skeptical. Has anyone else heard this? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vanessa Perez Sent: Friday, March 15, 2013 12:43 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Billing for Pin 4 Cocktail Its actually not allowed already. Any cocktail where all stains are done at one time on one slide can only be charged x1 even if its 2 or more antibodys in the cocktail. One of our reps updated us with our PIN cocktail -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Roger Heyna Sent: Friday, March 15, 2013 11:09 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Billing for Pin 4 Cocktail Our lab currently bills for three, and I know of other labs in our area that also bill for three. I have heard rumors that this may not be allowed in the near future. Roger Heyna Maywood, IL >>> Debbie Granato 3/15/2013 11:00 AM >>> We have a billing question for the PIN4 Cocktail that we perform on prostate needle biopsies. We currently use the Biocare CK5+CK14+p63 and then add the Biocare P 504S in the lab. How would you bill for this? Would billing for 1 stain be correct or can you bill for 3 stains? Thank you for your input. Debbie Granato _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. ------------------------------------------------------------------------- This message was secured by ZixCorp(R). From mhale <@t> MiracaLS.com Tue Mar 19 08:01:07 2013 From: mhale <@t> MiracaLS.com (Hale, Meredith) Date: Tue Mar 19 08:01:11 2013 Subject: [Histonet] KY HT Positions Message-ID: <0E828EC51C7CC445A51E53F81B64E8C71EA3D9@s-irv-exchmb.PathologyPartners.intranet> Please no recruiter calls Great opportunity for Histotechnician's in Crestview Hills, KY ! Tri-State Gastroenterology Associates is a multi-physician practice located in Northern Kentucky. Its mission is "To provide compassionate, high quality, cost-effective care to patients' with gastrointestinal problems" Looking for 2 Full Time HT/HTL's ( 32 hours a week is full time employment at this practice ) * Meet CLIA Grossing Requirements : CFR 493.1489, http://wwwn.cdc.gov/clia/regs/toc.aspx/ ,prior experience grossing GI specimens * Supervisor experience preferred * HT/HTL ASCP Certified * Experience with CLIA and CAP * Experience writing and maintaining policies and procedures * Prior laboratory start up experience is preferred * Ability to work independently Duties include: * Grossing * Embedding * Microtomy * Staining; routine and special stains only * Maintain supply orders and laboratory budget * Ability to be flexible and take on additional duties' as needed * Ability to work independently * Maintenance of laboratory for inspections * Maintenance of quality records Interested applicants should contact Meredith Hale; phone 214-596-2219 or through email mhale@miracals.com Meredith Hale HT (ASCP)cm Director External Sales Support Miraca Life Sciences 6655 North MacArthur Blvd. Irving , Texas 75039 Office: 214-596-2219 Cell: 469-648-8253 Fax: 1-866-688-3280 mhale@miracals.com> From Greg.Good <@t> tenethealth.com Mon Mar 18 13:11:17 2013 From: Greg.Good <@t> tenethealth.com (Good, Greg) Date: Tue Mar 19 08:01:47 2013 Subject: [Histonet] Lean Principles in Pathology Laboratory Message-ID: <5668023C7D6A4D4DAFF17BA7497FCCC56C791F29@TENHDCTHMB10-02.tenethealth.net> I am currently completing my capstone project through the University of Charleston and would greatly appreciate your help in taking a brief 5 minute survey. This survey is part of my action research paper and relates to various "Lean" methods which you may have applied in your histology laboratory. All respondents who include their email address will receive a copy of the research results upon completion to help identify how other histology labs have implemented various lean methodologies. Your email address will be used for no other purpose. If you choose not to receive this information, leave this field blank. Thanks! Click here to take survey or copy and paste the following link into your web browser. http://www.surveymonkey.com/s/J9TMZ73 Greg L. Good, HT(ASCP) Pathology Technical Manager Frye Regional Medical Center 420 North Center Street Hickory, North Carolina 28601 (828) 315-3680 (Office) (828) 315-3493 (Fax) greg.good@tenethealth.com NOTICE: The information in this communication is confidential and is directed only to the intended recipient. Please do not forward this communication without my permission. If you have received this communication in error, please notify me immediately. From Erin.Martin <@t> ucsf.edu Tue Mar 19 09:32:37 2013 From: Erin.Martin <@t> ucsf.edu (Martin, Erin) Date: Tue Mar 19 09:32:52 2013 Subject: [Histonet] IF on FFPE Message-ID: <24B7B291CC88D04AB663958E77A1F59D15684B@ex09.net.ucsf.edu> Hi everyone, Several people have contacted me regarding the article I mentioned in my post yesterday. Here are the details: Immunofluorescence With Dual Microwave Retrieval of Paraffin-Embedded Sections in the Assessment of Human Renal Biopsy Specimens AJCP 2013 139:71-78; doi:10.1309/AJCPRZG8EXN7BAID Suozhu Shi, Qingli Cheng, Ping Zhang, Nan Wang, Ying Zheng, Xue-Yuan Bai, and Xiangmei Chen Abstract: Immunofluorescence of frozen tissue sections (IF-F) is a classic technique for renal immunopathologic examination. However, it has certain disadvantages, such as diffuse antigen distribution and few or even no glomeruli in the section. We developed a new technique of immunofluorescence staining using dual microwave retrieval in paraffin-embedded renal tissue sections (IF-DMP) and compared IF-DMP with IF-F in 406 renal biopsy samples. IF-DMP detected significantly more glomeruli than did IF-F (P< .001). There was no significant difference for the specificity and sensitivity in the detection of immunoglobulins, complements, ?, and ? between IF-F and IF-DMP. Concordant observations were 98% for all immunofluorescence, complements, ?, and ? staining and 100% for immunoglobulin staining. Both techniques were completely accurate in confirming diagnoses of various glomerular diseases. IF-DMP provided clearer images of tissue structure and more precise localization of antigens, and it is a suitable alternative for traditional IF-F in clinical renal immunopathologic diagnosis. This is all foreign to me - we do IF on derm following an inherited protocol. I've never worked up any IF. If anyone has thoughts on how to apply this to skin I'd appreciate it! Thanks Erin Erin Martin, Histology Supervisor UCSF Dermatopathology Service 415-353-7248 Confidentiality Notice The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential and/or priviledged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited. If you receive this in error, please contact the sender and delete the material from any computer. From jtaylor <@t> meriter.com Tue Mar 19 11:18:39 2013 From: jtaylor <@t> meriter.com (Taylor, Jean) Date: Tue Mar 19 11:18:44 2013 Subject: [Histonet] p57 Message-ID: Hi everyone, Would anyone be willing to share information about what clone/company/dilution you use for p57? We are looking to use this antibody on formalin fixed/paraffin embedded human placenta. Thanks, Jean Taylor, HT(ASCP)QIHC Immuno Tech Meriter Health Services Madison, WI From Ronald.Houston <@t> nationwidechildrens.org Tue Mar 19 11:39:54 2013 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Tue Mar 19 11:42:36 2013 Subject: [Histonet] RE: p57 In-Reply-To: References: Message-ID: Jean, we use Leica's clone Kip2 on placentas on our Bonds; 1:150 Ronnie Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org "One person with passion is better than forty people merely interested." ~ E.M. Forster From: ihcrg@googlegroups.com [mailto:ihcrg@googlegroups.com] On Behalf Of Taylor, Jean Sent: Tuesday, March 19, 2013 12:19 PM To: 'histonet@lists.utsouthwestern.edu'; 'ihcrg@googlegroups.com' Subject: [IHCRG] p57 Hi everyone, Would anyone be willing to share information about what clone/company/dilution you use for p57? We are looking to use this antibody on formalin fixed/paraffin embedded human placenta. Thanks, Jean Taylor, HT(ASCP)QIHC Immuno Tech Meriter Health Services Madison, WI -- -- You received this message because you are subscribed to the Google Groups "ihcrg" group. The IHC Resource Group is a standing committee within the National Society for Histotechnology. To post to this group, send email to ihcrg@googlegroups.com To unsubscribe from this group, send email to ihcrg+unsubscribe@googlegroups.com For more options, visit this group at http://groups.google.com/group/ihcrg?hl=en To contact the National Society for Histotechnology, email: histo@nsh.org or call 443.535.4060. --- You received this message because you are subscribed to the Google Groups "ihcrg" group. To unsubscribe from this group and stop receiving emails from it, send an email to ihcrg+unsubscribe@googlegroups.com. For more options, visit https://groups.google.com/groups/opt_out. From Debra.Ortiz <@t> uchospitals.edu Tue Mar 19 12:22:12 2013 From: Debra.Ortiz <@t> uchospitals.edu (Debra.Ortiz@uchospitals.edu) Date: Tue Mar 19 12:23:03 2013 Subject: [Histonet] special stainers Message-ID: <3208937B4CD1CC45ADB5D8D4E6AA9FA24FC931@UCMCEXVS05HP.UCHAD.uchospitals.edu> Our histology laboratory is currently having problems with their specials stains; in particular the silver stains. Can you please tell me what instrumentation others in the field may be using for special stains? Thank you Debra A Ortiz, BS, MT, HT (ASCP) Technical Director, Anatomic Pathology The University of Chicago Medicine 5841 S. Maryland Ave. | Rm. S-631, MC6101 | Chicago, IL 60637 Office: 773-702-8492 Pager: 8633 AT THE FOREFRONT OF MEDICINE(r) http://www.uchospitals.edu http://www.uchicagokidshospital.org http://www.facebook.com/UChicagoMed Twitter: @UChicagoMed ******************************************************************************** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this e-mail message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is prohibited. If you have received this e-mail in error, please notify the sender and destroy all copies of the transmittal. Thank you University of Chicago Medical Center ******************************************************************************** From jqb7 <@t> cdc.gov Tue Mar 19 13:18:21 2013 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/OID/NCEZID)) Date: Tue Mar 19 14:16:26 2013 Subject: [Histonet] RE: special stainers In-Reply-To: <3208937B4CD1CC45ADB5D8D4E6AA9FA24FC931@UCMCEXVS05HP.UCHAD.uchospitals.edu> References: <3208937B4CD1CC45ADB5D8D4E6AA9FA24FC931@UCMCEXVS05HP.UCHAD.uchospitals.edu> Message-ID: We use the Dako Artisan. What silver stains are you wanting to automate? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Debra.Ortiz@uchospitals.edu Sent: Tuesday, March 19, 2013 1:22 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] special stainers Our histology laboratory is currently having problems with their specials stains; in particular the silver stains. Can you please tell me what instrumentation others in the field may be using for special stains? Thank you Debra A Ortiz, BS, MT, HT (ASCP) Technical Director, Anatomic Pathology The University of Chicago Medicine 5841 S. Maryland Ave. | Rm. S-631, MC6101 | Chicago, IL 60637 Office: 773-702-8492 Pager: 8633 AT THE FOREFRONT OF MEDICINE(r) http://www.uchospitals.edu http://www.uchicagokidshospital.org http://www.facebook.com/UChicagoMed Twitter: @UChicagoMed ******************************************************************************** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this e-mail message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is prohibited. If you have received this e-mail in error, please notify the sender and destroy all copies of the transmittal. Thank you University of Chicago Medical Center ******************************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SHUNTER <@t> beaumont.edu Tue Mar 19 09:32:06 2013 From: SHUNTER <@t> beaumont.edu (Sue Hunter) Date: Tue Mar 19 14:23:32 2013 Subject: [Histonet] RE: Immunofluorescence on FFPE skin In-Reply-To: <24B7B291CC88D04AB663958E77A1F59D1567D4@ex09.net.ucsf.edu> References: <24B7B291CC88D04AB663958E77A1F59D1567D4@ex09.net.ucsf.edu> Message-ID: Hi Erin We do direct immunofluorescence staining on FFPE kidney biopsies but instead of microwave antigen retrieval, we use proteinase K (20mg/ml Qiagen Cat # 19131) diluted 1/10 in Tris buffered saline for 20 minutes at Room Temp. We also incubate with the antibody (1/5 dilution) for 90 minutes. Don't remember if our student tried using the microwave (it was a student project) but I would certainly give it a try. There really is no difference with the wattage for a microwave - the trick is to get the solution boiling - THEN start your timing for retrieval. We do our AR for our FISH testing for 20 minutes at half power after it comes to a boil. This allows the solution to keep at near boiling temp but not to over flow the container when it comes back to a boil. The timing for using a pressure cooker would have to be validated - now you are adding an additional parameter of pressure to factor in to bring the solution to a boil. Good luck - hope it works for you. Sue Sue Hunter, Supervisor Advanced Diagnostics Beaumont Health System Royal Oak MI 248-898-5146 shunter@beaumont.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martin, Erin Sent: Monday, March 18, 2013 2:28 PM To: histonet Subject: [Histonet] Immunofluorescence on FFPE skin Hello all, My pathologist gave me a copy of "Immunofluorescence with Dual Microwave Retrieval of Paraffin-Embedded Sections in the Assessment of Human Renal Biopsy Specimens" from the American Journal of Clinical Pathology. He said that the same principle should work on skin and he would like to be able to do IF on fixed tissue in addition to our usual cryostat sections. Has anyone else read the paper who might be willing to give me some basic advice to try working it out? Is a microwave necessary (paper's method uses 2 different wattage settings) or is there a way to use HIER in waterbath or pressure cooker? Thanks in advance, Erin Erin Martin, Histology Supervisor UCSF Dermatopathology Service 415-353-7248 Confidentiality Notice The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential and/or priviledged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited. If you receive this in error, please contact the sender and delete the material from any computer. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BZIMMERM <@t> gru.edu Tue Mar 19 15:33:01 2013 From: BZIMMERM <@t> gru.edu (Zimmerman, Billie) Date: Tue Mar 19 15:33:06 2013 Subject: [Histonet] Georgia Society for Histotechnology Jekyll Island HISTOPALOOZA!! Message-ID: <7B3DEB32E69C034EACB479059C5DE3FF75C73C@EX-MLB-03.ad.georgiahealth.edu> There will be a general membership meeting while we are at Jekyll Island. We are asking all of you to help us plan the 2014 meeting. Next year's theme will be HISTOPALOOZA. A palooza is a big, promotional event that's fun and over the top. We plan to celebrate histotechnology in a big way. So, please join us! "You only live once, but if you do it right, once is enough." Mae West Wanda K. Simons President Michael Ayers Immediate Past President Michael Bourgeois Vice President Billie Zimmerman Secretary Shirley Powell Treasurer ***Tune in tomorrow for snippets of Billie's Travel Advisor for the Jekyll Island histology meeting. "no fluff, no sprinkles, just the facts." Billie Zimmerman MT(ASCP)QIHC GSH secretary (and Zumba gal) Augusta State University and Georgia Health Sciences University have consolidated to become Georgia Regents University. Effective January 9, 2013, my email address has changed to BZIMMERM@gru.edu. Please update your address book to reflect this change. From Vickroy.Jim <@t> mhsil.com Tue Mar 19 17:33:03 2013 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Tue Mar 19 17:33:10 2013 Subject: [Histonet] Validation of Her2 Message-ID: We are working on validating HER 2 testing in our laboratory. I see that the guidelines state that we should use 25 - 100 cases to complete our validation. In the past we sent our Her2 IHC testing to Clarient and they performed the technical testing and then our pathologists would do the scoring. So for validation we used 25 of the cases we sent to Clarient and then did them in house to compare. Our comparison was nearly 100%. Here is my question: Clarient uses the DAKO method while we are using Ventana's antibody Pathway (4B5). The validation guidelines state "parallel by identical method in another lab with the same validated assay is also acceptable". Can I assume that this means comparison with another lab for IHC HER2 testing and does not have to be using the same clone (DAKO vs Ventana)? Jim James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From JEllin <@t> yumaregional.org Tue Mar 19 19:30:23 2013 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Tue Mar 19 20:21:23 2013 Subject: [Histonet] Validation of Her2 In-Reply-To: References: Message-ID: <420A2ED8-1674-4D57-AEA1-773A7A195232@yumaregional.org> What did you do to evaluate your system and algorithms. Also the cases of ER and PR. Negative vs Postive concordance. Sent from my iPad On Mar 19, 2013, at 4:57 PM, "Vickroy, Jim" wrote: > > We are working on validating HER 2 testing in our laboratory. I see that the guidelines state that we should use 25 - 100 cases to complete our validation. > In the past we sent our Her2 IHC testing to Clarient and they performed the technical testing and then our pathologists would do the scoring. So for validation we used 25 of the cases we sent to Clarient and then did them in house to compare. Our comparison was nearly 100%. Here is my question: > > Clarient uses the DAKO method while we are using Ventana's antibody Pathway (4B5). The validation guidelines state "parallel by identical method in another lab with the same validated assay is also acceptable". Can I assume that this means comparison with another lab for IHC HER2 testing and does not have to be using the same clone (DAKO vs Ventana)? > > Jim > > James Vickroy BS, HT(ASCP) > > Surgical and Autopsy Pathology Technical Supervisor > Memorial Medical Center > 217-788-4046 > > > ________________________________ > This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ From lins0701 <@t> gmail.com Tue Mar 19 21:31:49 2013 From: lins0701 <@t> gmail.com (Sophia Lin) Date: Tue Mar 19 21:31:53 2013 Subject: [Histonet] H&E Stainer Leica vs Sakura Message-ID: Hi, We are currently looking to switch out our linear MKII stainer for either a Leica XL autostainer or the Sakura Tissue-Tek Prisma. Any recommendations? Are quantity of H&Es is increasing and we need adequate equipment to meet our workload. The incorporated oven seems excellent on both stainers. Any pros/cons would be greatly appreciated! Also, if you are currently using the stainer, does it meet your workload and what is your volume? Thanks! Sophia From PREISZNE <@t> mail.etsu.edu Wed Mar 20 08:14:07 2013 From: PREISZNE <@t> mail.etsu.edu (Preiszner, Johanna) Date: Wed Mar 20 08:14:34 2013 Subject: [Histonet] RE: IF on FFPE In-Reply-To: <0B25F4E5EE083249BB5E23012ACC3B1885AD514C@etsums4.etsu.edu> References: <24B7B291CC88D04AB663958E77A1F59D15684B@ex09.net.ucsf.edu>, <0B25F4E5EE083249BB5E23012ACC3B1885AD514C@etsums4.etsu.edu> Message-ID: <0B25F4E5EE083249BB5E23012ACC3B1885AD5205@etsums4.etsu.edu> Hi Netters, I've read the paper, but it's not clear what temperature they reached during the first round of HIER. They do not give the volume of the first HIER solution, only the time and wattage of the microwave treatment. And which procedure do they call "first" and "second"? It looks different in the "Methods" section from what they mean in the "Discussion". Hanna Preiszner ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Martin, Erin [Erin.Martin@ucsf.edu] Sent: Tuesday, March 19, 2013 10:32 AM To: histonet Subject: [Histonet] IF on FFPE Hi everyone, Several people have contacted me regarding the article I mentioned in my post yesterday. Here are the details: Immunofluorescence With Dual Microwave Retrieval of Paraffin-Embedded Sections in the Assessment of Human Renal Biopsy Specimens AJCP 2013 139:71-78; doi:10.1309/AJCPRZG8EXN7BAID Suozhu Shi, Qingli Cheng, Ping Zhang, Nan Wang, Ying Zheng, Xue-Yuan Bai, and Xiangmei Chen Abstract: Immunofluorescence of frozen tissue sections (IF-F) is a classic technique for renal immunopathologic examination. However, it has certain disadvantages, such as diffuse antigen distribution and few or even no glomeruli in the section. We developed a new technique of immunofluorescence staining using dual microwave retrieval in paraffin-embedded renal tissue sections (IF-DMP) and compared IF-DMP with IF-F in 406 renal biopsy samples. IF-DMP detected significantly more glomeruli than did IF-F (P< .001). There was no significant difference for the specificity and sensitivity in the detection of immunoglobulins, complements, ?, and ? between IF-F and IF-DMP. Concordant observations were 98% for all immunofluorescence, complements, ?, and ? staining and 100% for immunoglobulin staining. Both techniques were completely accurate in confirming diagnoses of various glomerular diseases. IF-DMP provided clearer images of tissue structure and more precise localization of antigens, and it is a suitable alternative for traditional IF-F in clinical renal immunopathologic diagnosis. This is all foreign to me - we do IF on derm following an inherited protocol. I've never worked up any IF. If anyone has thoughts on how to apply this to skin I'd appreciate it! Thanks Erin Erin Martin, Histology Supervisor UCSF Dermatopathology Service 415-353-7248 Confidentiality Notice The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential and/or priviledged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited. If you receive this in error, please contact the sender and delete the material from any computer. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kdwyer3322 <@t> aol.com Wed Mar 20 08:21:53 2013 From: kdwyer3322 <@t> aol.com (kdwyer3322@aol.com) Date: Wed Mar 20 08:22:48 2013 Subject: [Histonet] Solving the Puzzle...one piece at a Time - Texas Society for Histotechnology Symposium Message-ID: <8CFF38505160978-1104-319CC@Webmail-d116.sysops.aol.com> All, It's not to late to sign up for the Texas Society for Histotechnology State Symposium/Convention. Our meeting will include a career day for area high school students, slide contest for the HT students and a poster session. Attendees will get a chance to visit over 30 vendors and learn new techniques from 20 workshops to choose from. The meeting will be held at: J.W. Marriott, 5150 Westheimer Road, Houston, Texas When booking a room please let the hotel know you are with the Texas Society for Histotechnology We hope to see you in Houston!! From ihcman2010 <@t> hotmail.com Wed Mar 20 11:06:07 2013 From: ihcman2010 <@t> hotmail.com (Glen Dawson) Date: Wed Mar 20 11:06:11 2013 Subject: [Histonet] Tri-State Histology Symposium Message-ID: All, I am forwarding this to the histonet so that you have the opportunity to attend one of the best state conventions in the nation. Hope to see you there. Glen Dawson BS, HT(ASCP), QIHCWisconsin Histology Society President From: Mitchell Jean A [mailto:JMitchell@uwhealth.org] 2013 Tri-State Histology SymposiumDear Histonetters: You are invited to participate in the Iowa, Wisconsin and Minnesota Tri-State Spring Histology Symposium that will be held at the Hotel Julien, Dubuque, Iowa April 24-26th. There is an outstanding program of seminars and workshops that begin Wednesday afternoon April 24th and conclude at noon on Friday April 26th. Join us for education, vendor displays, socializing and histotech camaraderie as we look to a "Roaring Return to Dubuque" in 2013. For program, registration and vendor/exhibit information contact the following representatives: Iowa: Judi Stasko (judith.stasko@ars.usda.gov) Wisconsin: Dawn Schneider (dawn.schneider@ministryhealth.org) Minnesota: Sheri Blair (sheriblair1@netzero.net) Vendor/Exhibit: Dawn Schneider (dawn.schneider@ministryhealth.org) From Ashley.Troutman <@t> Vanderbilt.Edu Wed Mar 20 12:31:36 2013 From: Ashley.Troutman <@t> Vanderbilt.Edu (Troutman, Kenneth A) Date: Wed Mar 20 12:31:50 2013 Subject: [Histonet] Validation of Her2 Message-ID: Hi Jim, The way I understand this guideline, you will need to send it to a lab that uses the same Ventana clone and instrument that you use. Regards, Ashley Troutman BS, HT(ASCP) QIHC Immunohistochemistry Supervisor Vanderbilt University Histopathology 1301 Medical Center Drive TVC 4531 Nashville, TN 37232 ashley.troutman@vanderbilt.edu Message: 5 Date: Tue, 19 Mar 2013 17:33:03 -0500 From: "Vickroy, Jim" > Subject: [Histonet] Validation of Her2 To: "histonet@lists.utsouthwestern.edu" > Message-ID: > Content-Type: text/plain; charset="us-ascii" We are working on validating HER 2 testing in our laboratory. I see that the guidelines state that we should use 25 - 100 cases to complete our validation. In the past we sent our Her2 IHC testing to Clarient and they performed the technical testing and then our pathologists would do the scoring. So for validation we used 25 of the cases we sent to Clarient and then did them in house to compare. Our comparison was nearly 100%. Here is my question: Clarient uses the DAKO method while we are using Ventana's antibody Pathway (4B5). The validation guidelines state "parallel by identical method in another lab with the same validated assay is also acceptable". Can I assume that this means comparison with another lab for IHC HER2 testing and does not have to be using the same clone (DAKO vs Ventana)? Jim James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 From gu.lang <@t> gmx.at Wed Mar 20 12:43:42 2013 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Wed Mar 20 12:43:47 2013 Subject: AW: [Histonet] Immunofluorescence on FFPE skin In-Reply-To: <24B7B291CC88D04AB663958E77A1F59D1567D4@ex09.net.ucsf.edu> References: <24B7B291CC88D04AB663958E77A1F59D1567D4@ex09.net.ucsf.edu> Message-ID: <000901ce2592$770a4de0$651ee9a0$@gmx.at> HI Erin, we tried to do the usual IHC-protocol for immunoglobulins on fixed skin-biopsies for Pemphigus. (on Ventana Benchmark, with HIER in high pH retrieval buffer). It gave very, very overstained sections. We didn't continue this project, because usual IF is easier to "fix" , has worked "for ever" and the results are typically described in pathology-literature. But I think it could work, if you make a really high dilution of the antibodies. And in mind of the brown melanin-pigment, a red chromogen would give a better contrast. In my opinion microwave plays no role - it just has to be HIER. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Martin, Erin Gesendet: Montag, 18. M?rz 2013 19:28 An: histonet Betreff: [Histonet] Immunofluorescence on FFPE skin Hello all, My pathologist gave me a copy of "Immunofluorescence with Dual Microwave Retrieval of Paraffin-Embedded Sections in the Assessment of Human Renal Biopsy Specimens" from the American Journal of Clinical Pathology. He said that the same principle should work on skin and he would like to be able to do IF on fixed tissue in addition to our usual cryostat sections. Has anyone else read the paper who might be willing to give me some basic advice to try working it out? Is a microwave necessary (paper's method uses 2 different wattage settings) or is there a way to use HIER in waterbath or pressure cooker? Thanks in advance, Erin Erin Martin, Histology Supervisor UCSF Dermatopathology Service 415-353-7248 Confidentiality Notice The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential and/or priviledged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited. If you receive this in error, please contact the sender and delete the material from any computer. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Marilyn.A.Weiss <@t> kp.org Wed Mar 20 14:05:54 2013 From: Marilyn.A.Weiss <@t> kp.org (Marilyn.A.Weiss@kp.org) Date: Wed Mar 20 14:06:16 2013 Subject: [Histonet] question Message-ID: Thank you for your response about the squames and we are all wearing gloves now, if one uses them then we all do is my motto, although in 50 years of cutting, this is the first time this has come up. . guess I was lucky. anyway, the same pathologists would like to know if there is a standard about wearing gloves in the histo world and if so, is it because of squames or although the tissue is fixed, is there chances of infections or something else. thank you. NOTICE TO RECIPIENT: If you are not the intended recipient of this e-mail, you are prohibited from sharing, copying, or otherwise using or disclosing its contents. If you have received this e-mail in error, please notify the sender immediately by reply e-mail and permanently delete this e-mail and any attachments without reading, forwarding or saving them. Thank you. From LSebree <@t> uwhealth.org Wed Mar 20 16:07:44 2013 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Wed Mar 20 16:07:48 2013 Subject: [Histonet] Glutamine Synthetase and HSP 70 IHC test availability Message-ID: <77DD817201982748BC67D7960F2F76AF03E3C3@UWHC-MBX12.uwhis.hosp.wisc.edu> Hello Histonetters, One of our pathologists is requesting Glutamine Synthetase and Heat Shock Protein (HSP) 70 immunostains on a liver specimen. Our usual "go to" reference labs do not perform these. Are there labs out there that have these tests available? Even non-reference labs??? Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 From abright <@t> brightinstruments.com Wed Mar 20 16:37:45 2013 From: abright <@t> brightinstruments.com (Alan Bright) Date: Wed Mar 20 16:38:11 2013 Subject: [Histonet] question In-Reply-To: References: Message-ID: <80277E19-1C15-48BE-82F9-5BC079622D2B@brightinstruments.com> Strange about the gloves when vacuuming out fresh tissue trimmings from cryostats and exhausting the air back into the lab is done. Alan Bright On 20 Mar 2013, at 19:07, "Marilyn.A.Weiss@kp.org" wrote: > Thank you for your response about the squames and we are all wearing > gloves now, if one uses them then we all do is my motto, although in 50 > years of cutting, this is the first time this has come up. . guess I was > lucky. anyway, the same pathologists would like to know if there is a > standard about wearing gloves in the histo world and if so, is it because > of squames or although the tissue is fixed, is there chances of infections > or something else. thank you. > > NOTICE TO RECIPIENT: If you are not the intended recipient of this > e-mail, you are prohibited from sharing, copying, or otherwise using or > disclosing its contents. If you have received this e-mail in error, > please notify the sender immediately by reply e-mail and permanently > delete this e-mail and any attachments without reading, forwarding or > saving them. Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > From Rcartun <@t> harthosp.org Wed Mar 20 16:49:23 2013 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Wed Mar 20 16:51:08 2013 Subject: [Histonet] Glutamine Synthetase and HSP 70 IHC test availability In-Reply-To: <77DD817201982748BC67D7960F2F76AF03E3C3@UWHC-MBX12.uwhis.hosp.wisc.edu> References: <77DD817201982748BC67D7960F2F76AF03E3C3@UWHC-MBX12.uwhis.hosp.wisc.edu> Message-ID: <5149F6A3.7770.0077.1@harthosp.org> We are doing these markers, but, to be perfectly honest, I am not very impressed with their results. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-2204 Fax >>> Sebree Linda A 3/20/2013 5:07 PM >>> Hello Histonetters, One of our pathologists is requesting Glutamine Synthetase and Heat Shock Protein (HSP) 70 immunostains on a liver specimen. Our usual "go to" reference labs do not perform these. Are there labs out there that have these tests available? Even non-reference labs??? Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpwenk <@t> sbcglobal.net Wed Mar 20 17:45:22 2013 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Wed Mar 20 17:45:29 2013 Subject: [Histonet] Best Carmine stain In-Reply-To: <8D2ECF37-6661-478D-886C-566493FDA66A@sbcglobal.net> References: <8D2ECF37-6661-478D-886C-566493FDA66A@sbcglobal.net> Message-ID: If you are looking for glycogen - so a PAS. More sensitive and a deeper magenta color than the Best Carmine. Curious, what is "without water"? - The Frozen section????? But there's water in the cells, which is what is freezing. - The stain?????? But most stains are made in water (aqueous stains). Why do you need no water????? Peggy Wenk, HTL(ASCP)SLS -----Original Message----- From: Kiranjit Grewal Sent: Saturday, March 16, 2013 11:39 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Best Carmine stain Hello, Any tips on doing Best Carmine on Frozen sections without water. Can we use mucicarmine solution to pick up glycogen in heart muscle? Thank u, Kiran Sent from my iPhone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From contact <@t> histocare.com Wed Mar 20 17:49:22 2013 From: contact <@t> histocare.com (Contact HistoCare) Date: Wed Mar 20 17:50:13 2013 Subject: [Histonet] H&E Stainer Leica vs Sakura (Sophia Lin) In-Reply-To: <201303201704.r2KH4UhM024333@mail20c40.carrierzone.com> References: <201303201704.r2KH4UhM024333@mail20c40.carrierzone.com> Message-ID: <3A581710-3E83-49F4-B325-96DF378BE506@histocare.com> Both stainers are powerhouses. The Leica has a plain menu screen with a simple interface while the Sakura has a LCD screen with detailed information about what stage the staining process a rack is along with multiple menus. The difference between the performance changes drastically when the respective coverslipper attachments become involved. The Leica is seriously no match for the Sakura in this respect. The Leica's coverslipper is its Achilles heel and requires a LOT more attention and alerts frequently, very frequently. It takes a separate rack for staining the slides at the beginning of the process and eventually transfers them to a different rack one the cover slip is complete. This one uses glass and frequently drops glass, creates bubbles, drops and breaks slides. You will have to frequently purge the system and clean the cover medium needle dropper. Once done, it only holds. Two racks of 30 slides and will alert until you remove it. You can't leave this one alone for more than 5 minutes without an alert. Seriously. The Sakura's coverslipper uses cover tape which won't need to be replaced not even remotely as soon as the glass in the Leica. Finished slides remain in a carousel at the top and can hold about 10 racks of 20 before it alerts. For high volume, the Sakura pair wins hands down. You won't lose productivity time by needing to check on this machine pair. HistoCare.com Hi, We are currently looking to switch out our linear MKII stainer for either a Leica XL autostainer or the Sakura Tissue-Tek Prisma. Any recommendations? Are quantity of H&Es is increasing and we need adequate equipment to meet our workload. The incorporated oven seems excellent on both stainers. Any pros/cons would be greatly appreciated! Also, if you are currently using the stainer, does it meet your workload and what is your volume? Thanks! Sophia From lwhite38 <@t> cogeco.ca Wed Mar 20 18:07:54 2013 From: lwhite38 <@t> cogeco.ca (Lori White) Date: Wed Mar 20 18:08:12 2013 Subject: [Histonet] RE: H&E Stainer Leica vs Sakura In-Reply-To: <0MJY006CHY1OFLI0@busymta01.int.cogeco.net> References: <0MJY006CHY1OFLI0@busymta01.int.cogeco.net> Message-ID: <000301ce25bf$c4029d50$4c07d7f0$@ca> Hi Sophia, I am a big fan of the Prisma stainer. We have two of them - one for routine staining, linked to the coverslipper and the other is used for Cytology and some automated special stains. We have had them for five years with no problems other than the replacement of the door latches. Lori ------------------------------ Message: 7 Date: Tue, 19 Mar 2013 19:31:49 -0700 From: Sophia Lin Subject: [Histonet] H&E Stainer Leica vs Sakura To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hi, We are currently looking to switch out our linear MKII stainer for either a Leica XL autostainer or the Sakura Tissue-Tek Prisma. Any recommendations? Are quantity of H&Es is increasing and we need adequate equipment to meet our workload. The incorporated oven seems excellent on both stainers. Any pros/cons would be greatly appreciated! Also, if you are currently using the stainer, does it meet your workload and what is your volume? Thanks! Sophia From joelleweaver <@t> hotmail.com Wed Mar 20 18:32:07 2013 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Wed Mar 20 18:33:42 2013 Subject: [Histonet] H&E Stainer Leica vs Sakura (Sophia Lin) In-Reply-To: <3A581710-3E83-49F4-B325-96DF378BE506@histocare.com> References: <201303201704.r2KH4UhM024333@mail20c40.carrierzone.com>, <3A581710-3E83-49F4-B325-96DF378BE506@histocare.com> Message-ID: I agree. The Leica stainer and Sakura are both good instruments, however I really HATE that Leica glass cover slipper and I had the same assessment as to need to "baby sit". I sometimes preferred just hand cover slipping because it was less trouble, and even faster sometimes ( believe that or not). I had none of these issues with the Prisma covcr slipper, and no trouble with the user interface etc. I had the same issue with the door latch on the Prisma, but other than a quick replacement of that, it worked perfectly. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: contact@histocare.com > Date: Wed, 20 Mar 2013 17:49:22 -0500 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] H&E Stainer Leica vs Sakura (Sophia Lin) > > Both stainers are powerhouses. The Leica has a plain menu screen with a simple interface while the Sakura has a LCD screen with detailed information about what stage the staining process a rack is along with multiple menus. The difference between the performance changes drastically when the respective coverslipper attachments become involved. > > The Leica is seriously no match for the Sakura in this respect. The Leica's coverslipper is its Achilles heel and requires a LOT more attention and alerts frequently, very frequently. It takes a separate rack for staining the slides at the beginning of the process and eventually transfers them to a different rack one the cover slip is complete. This one uses glass and frequently drops glass, creates bubbles, drops and breaks slides. You will have to frequently purge the system and clean the cover medium needle dropper. Once done, it only holds. Two racks of 30 slides and will alert until you remove it. You can't leave this one alone for more than 5 minutes without an alert. Seriously. > > The Sakura's coverslipper uses cover tape which won't need to be replaced not even remotely as soon as the glass in the Leica. Finished slides remain in a carousel at the top and can hold about 10 racks of 20 before it alerts. For high volume, the Sakura pair wins hands down. You won't lose productivity time by needing to check on this machine pair. > > HistoCare.com > > > > > > > Hi, > > We are currently looking to switch out our linear MKII stainer for either a > Leica XL autostainer or the Sakura Tissue-Tek Prisma. Any recommendations? > Are quantity of H&Es is increasing and we need adequate equipment to meet > our workload. The incorporated oven seems excellent on both stainers. Any > pros/cons would be greatly appreciated! Also, if you are currently using > the stainer, does it meet your workload and what is your volume? > > Thanks! > > Sophia > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Wed Mar 20 18:38:04 2013 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Wed Mar 20 18:38:12 2013 Subject: [Histonet] question In-Reply-To: <80277E19-1C15-48BE-82F9-5BC079622D2B@brightinstruments.com> References: , <80277E19-1C15-48BE-82F9-5BC079622D2B@brightinstruments.com> Message-ID: Maybe that is why some like the UV decontamination option? I know I prefer it. If there is aerolizing of any material or particles after that cycle, at least it whatever was trapped in the chamber would have been decontaminated when you then vaccum. I think that the UV models have their own vaccum as I recall. Plus it is MUCH faster than the old defrots, wipe out, vaccum and rinse steps and air dry steps needed to decontaminate some ( usually older) cryostats. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: abright@brightinstruments.com > Date: Wed, 20 Mar 2013 21:37:45 +0000 > To: Marilyn.A.Weiss@kp.org > Subject: Re: [Histonet] question > CC: histonet@lists.utsouthwestern.edu > > Strange about the gloves when vacuuming out fresh tissue trimmings from cryostats and exhausting the air back into the lab is done. > > Alan Bright > > On 20 Mar 2013, at 19:07, "Marilyn.A.Weiss@kp.org" wrote: > > > Thank you for your response about the squames and we are all wearing > > gloves now, if one uses them then we all do is my motto, although in 50 > > years of cutting, this is the first time this has come up. . guess I was > > lucky. anyway, the same pathologists would like to know if there is a > > standard about wearing gloves in the histo world and if so, is it because > > of squames or although the tissue is fixed, is there chances of infections > > or something else. thank you. > > > > NOTICE TO RECIPIENT: If you are not the intended recipient of this > > e-mail, you are prohibited from sharing, copying, or otherwise using or > > disclosing its contents. If you have received this e-mail in error, > > please notify the sender immediately by reply e-mail and permanently > > delete this e-mail and any attachments without reading, forwarding or > > saving them. Thank you. > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > -- > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Wed Mar 20 18:39:48 2013 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Wed Mar 20 18:40:58 2013 Subject: [Histonet] question In-Reply-To: References: , , <80277E19-1C15-48BE-82F9-5BC079622D2B@brightinstruments.com>, Message-ID: sorry for the misspelling in my previous post. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: joelleweaver@hotmail.com > To: abright@brightinstruments.com; marilyn.a.weiss@kp.org > Date: Wed, 20 Mar 2013 23:38:04 +0000 > Subject: RE: [Histonet] question > CC: histonet@lists.utsouthwestern.edu > > Maybe that is why some like the UV decontamination option? I know I prefer it. If there is aerolizing of any material or particles after that cycle, at least it whatever was trapped in the chamber would have been decontaminated when you then vaccum. I think that the UV models have their own vaccum as I recall. Plus it is MUCH faster than the old defrots, wipe out, vaccum and rinse steps and air dry steps needed to decontaminate some ( usually older) cryostats. > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > From: abright@brightinstruments.com > > Date: Wed, 20 Mar 2013 21:37:45 +0000 > > To: Marilyn.A.Weiss@kp.org > > Subject: Re: [Histonet] question > > CC: histonet@lists.utsouthwestern.edu > > > > Strange about the gloves when vacuuming out fresh tissue trimmings from cryostats and exhausting the air back into the lab is done. > > > > Alan Bright > > > > On 20 Mar 2013, at 19:07, "Marilyn.A.Weiss@kp.org" wrote: > > > > > Thank you for your response about the squames and we are all wearing > > > gloves now, if one uses them then we all do is my motto, although in 50 > > > years of cutting, this is the first time this has come up. . guess I was > > > lucky. anyway, the same pathologists would like to know if there is a > > > standard about wearing gloves in the histo world and if so, is it because > > > of squames or although the tissue is fixed, is there chances of infections > > > or something else. thank you. > > > > > > NOTICE TO RECIPIENT: If you are not the intended recipient of this > > > e-mail, you are prohibited from sharing, copying, or otherwise using or > > > disclosing its contents. If you have received this e-mail in error, > > > please notify the sender immediately by reply e-mail and permanently > > > delete this e-mail and any attachments without reading, forwarding or > > > saving them. Thank you. > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > > -- > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Wed Mar 20 21:40:34 2013 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/OID/NCEZID)) Date: Wed Mar 20 21:41:26 2013 Subject: [Histonet] H&E Stainer Leica vs Sakura (Sophia Lin) In-Reply-To: <3A581710-3E83-49F4-B325-96DF378BE506@histocare.com> References: <201303201704.r2KH4UhM024333@mail20c40.carrierzone.com> <3A581710-3E83-49F4-B325-96DF378BE506@histocare.com> Message-ID: We have both and love them both. IF you are using tape coverslips then perhaps Sakura is your best bet. We use glass coverslips on BOTH the Leica and Sakura and find fewer problems with the Leica. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Contact HistoCare Sent: Wednesday, March 20, 2013 6:49 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] H&E Stainer Leica vs Sakura (Sophia Lin) Both stainers are powerhouses. The Leica has a plain menu screen with a simple interface while the Sakura has a LCD screen with detailed information about what stage the staining process a rack is along with multiple menus. The difference between the performance changes drastically when the respective coverslipper attachments become involved. The Leica is seriously no match for the Sakura in this respect. The Leica's coverslipper is its Achilles heel and requires a LOT more attention and alerts frequently, very frequently. It takes a separate rack for staining the slides at the beginning of the process and eventually transfers them to a different rack one the cover slip is complete. This one uses glass and frequently drops glass, creates bubbles, drops and breaks slides. You will have to frequently purge the system and clean the cover medium needle dropper. Once done, it only holds. Two racks of 30 slides and will alert until you remove it. You can't leave this one alone for more than 5 minutes without an alert. Seriously. The Sakura's coverslipper uses cover tape which won't need to be replaced not even remotely as soon as the glass in the Leica. Finished slides remain in a carousel at the top and can hold about 10 racks of 20 before it alerts. For high volume, the Sakura pair wins hands down. You won't lose productivity time by needing to check on this machine pair. HistoCare.com Hi, We are currently looking to switch out our linear MKII stainer for either a Leica XL autostainer or the Sakura Tissue-Tek Prisma. Any recommendations? Are quantity of H&Es is increasing and we need adequate equipment to meet our workload. The incorporated oven seems excellent on both stainers. Any pros/cons would be greatly appreciated! Also, if you are currently using the stainer, does it meet your workload and what is your volume? Thanks! Sophia _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lins0701 <@t> gmail.com Wed Mar 20 22:51:42 2013 From: lins0701 <@t> gmail.com (Sophia Lin) Date: Wed Mar 20 22:52:59 2013 Subject: [Histonet] Leica XL autostainer in California? Message-ID: Hi, Thanks for the responses regarding the Leica XL and the Sakura Tissue Tek Prisma! Just wondering if anyone in Southern California (Inland Empire/Los Angeles) area has the Leica XL stainer? My tech and I would love the opportunity to see it in action and to see it before we decide which to purchase. Please let me know if anyone in the southern california area has the Leica XL and would allow us to stop by to see it! We would love to treat you to lunch for your help! Thanks! Sophia From abright <@t> brightinstruments.com Thu Mar 21 05:25:41 2013 From: abright <@t> brightinstruments.com (Alan Bright) Date: Thu Mar 21 05:26:44 2013 Subject: [Histonet] question In-Reply-To: References: <80277E19-1C15-48BE-82F9-5BC079622D2B@brightinstruments.com> Message-ID: <928F2BCD-1482-489E-8056-6BD1761BE8C7@brightinstruments.com> I do not see that as UV will not decontaminate areas and tissue that are not in the UV light path or thick tissue. Alan Bright Sent from my iPhone On 20 Mar 2013, at 23:38, "joelle weaver" wrote: > Maybe that is why some like the UV decontamination option? I know I prefer it. If there is aerolizing of any material or particles after that cycle, at least it whatever was trapped in the chamber would have been decontaminated when you then vaccum. I think that the UV models have their own vaccum as I recall. Plus it is MUCH faster than the old defrots, wipe out, vaccum and rinse steps and air dry steps needed to decontaminate some ( usually older) cryostats. > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > From: abright@brightinstruments.com > > Date: Wed, 20 Mar 2013 21:37:45 +0000 > > To: Marilyn.A.Weiss@kp.org > > Subject: Re: [Histonet] question > > CC: histonet@lists.utsouthwestern.edu > > > > Strange about the gloves when vacuuming out fresh tissue trimmings from cryostats and exhausting the air back into the lab is done. > > > > Alan Bright > > > > On 20 Mar 2013, at 19:07, "Marilyn.A.Weiss@kp.org" wrote: > > > > > Thank you for your response about the squames and we are all wearing > > > gloves now, if one uses them then we all do is my motto, although in 50 > > > years of cutting, this is the first time this has come up. . guess I was > > > lucky. anyway, the same pathologists would like to know if there is a > > > standard about wearing gloves in the histo world and if so, is it because > > > of squames or although the tissue is fixed, is there chances of infections > > > or something else. thank you. > > > > > > NOTICE TO RECIPIENT: If you are not the intended recipient of this > > > e-mail, you are prohibited from sharing, copying, or otherwise using or > > > disclosing its contents. If you have received this e-mail in error, > > > please notify the sender immediately by reply e-mail and permanently > > > delete this e-mail and any attachments without reading, forwarding or > > > saving them. Thank you. > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > > -- > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Spam > Not spam > Forget previous vote From akbitting <@t> geisinger.edu Thu Mar 21 07:04:27 2013 From: akbitting <@t> geisinger.edu (Bitting, Angela K.) Date: Thu Mar 21 07:04:40 2013 Subject: [Histonet] H&E Stainer Leica vs Sakura (Sophia Lin) In-Reply-To: References: <201303201704.r2KH4UhM024333@mail20c40.carrierzone.com>, <3A581710-3E83-49F4-B325-96DF378BE506@histocare.com> Message-ID: <77F52EFAB8B1694B885E277C48FCD0F639B5C6F1@GHSEXMBX2W8K1V.geisinger.edu> We hate Leica's CV5030 coverslipper too.!!! "Babysit" is a good word to describe the user experience. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelle weaver Sent: Wednesday, March 20, 2013 7:32 PM To: Contact HistoCare; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] H&E Stainer Leica vs Sakura (Sophia Lin) I agree. The Leica stainer and Sakura are both good instruments, however I really HATE that Leica glass cover slipper and I had the same assessment as to need to "baby sit". I sometimes preferred just hand cover slipping because it was less trouble, and even faster sometimes ( believe that or not). I had none of these issues with the Prisma covcr slipper, and no trouble with the user interface etc. I had the same issue with the door latch on the Prisma, but other than a quick replacement of that, it worked perfectly. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: contact@histocare.com > Date: Wed, 20 Mar 2013 17:49:22 -0500 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] H&E Stainer Leica vs Sakura (Sophia Lin) > > Both stainers are powerhouses. The Leica has a plain menu screen with a simple interface while the Sakura has a LCD screen with detailed information about what stage the staining process a rack is along with multiple menus. The difference between the performance changes drastically when the respective coverslipper attachments become involved. > > The Leica is seriously no match for the Sakura in this respect. The Leica's coverslipper is its Achilles heel and requires a LOT more attention and alerts frequently, very frequently. It takes a separate rack for staining the slides at the beginning of the process and eventually transfers them to a different rack one the cover slip is complete. This one uses glass and frequently drops glass, creates bubbles, drops and breaks slides. You will have to frequently purge the system and clean the cover medium needle dropper. Once done, it only holds. Two racks of 30 slides and will alert until you remove it. You can't leave this one alone for more than 5 minutes without an alert. Seriously. > > The Sakura's coverslipper uses cover tape which won't need to be replaced not even remotely as soon as the glass in the Leica. Finished slides remain in a carousel at the top and can hold about 10 racks of 20 before it alerts. For high volume, the Sakura pair wins hands down. You won't lose productivity time by needing to check on this machine pair. > > HistoCare.com > > > > > > > Hi, > > We are currently looking to switch out our linear MKII stainer for > either a Leica XL autostainer or the Sakura Tissue-Tek Prisma. Any recommendations? > Are quantity of H&Es is increasing and we need adequate equipment to > meet our workload. The incorporated oven seems excellent on both > stainers. Any pros/cons would be greatly appreciated! Also, if you are > currently using the stainer, does it meet your workload and what is your volume? > > Thanks! > > Sophia > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. ------------------------------------------------------------------------- This message was secured by ZixCorp(R). From joelleweaver <@t> hotmail.com Thu Mar 21 07:10:31 2013 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Thu Mar 21 07:10:40 2013 Subject: [Histonet] question In-Reply-To: <928F2BCD-1482-489E-8056-6BD1761BE8C7@brightinstruments.com> References: <80277E19-1C15-48BE-82F9-5BC079622D2B@brightinstruments.com> , <928F2BCD-1482-489E-8056-6BD1761BE8C7@brightinstruments.com> Message-ID: I guess I didn't understand what debris you were speaking of, yes it would only decontaminate what is within the chamber. I guess I never had much issue with any "blowing" Joelle Weaver MAOM, HTL (ASCP) QIHC CC: marilyn.a.weiss@kp.org; histonet@lists.utsouthwestern.edu From: abright@brightinstruments.com Subject: Re: [Histonet] question Date: Thu, 21 Mar 2013 10:25:41 +0000 To: joelleweaver@hotmail.com I do not see that as UV will not decontaminate areas and tissue that are not in the UV light path or thick tissue.Alan Bright Sent from my iPhone On 20 Mar 2013, at 23:38, "joelle weaver" wrote: Maybe that is why some like the UV decontamination option? I know I prefer it. If there is aerolizing of any material or particles after that cycle, at least it whatever was trapped in the chamber would have been decontaminated when you then vaccum. I think that the UV models have their own vaccum as I recall. Plus it is MUCH faster than the old defrots, wipe out, vaccum and rinse steps and air dry steps needed to decontaminate some ( usually older) cryostats. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: abright@brightinstruments.com > Date: Wed, 20 Mar 2013 21:37:45 +0000 > To: Marilyn.A.Weiss@kp.org > Subject: Re: [Histonet] question > CC: histonet@lists.utsouthwestern.edu > > Strange about the gloves when vacuuming out fresh tissue trimmings from cryostats and exhausting the air back into the lab is done. > > Alan Bright > > On 20 Mar 2013, at 19:07, "Marilyn.A.Weiss@kp.org" wrote: > > > Thank you for your response about the squames and we are all wearing > > gloves now, if one uses them then we all do is my motto, although in 50 > > years of cutting, this is the first time this has come up. . guess I was > > lucky. anyway, the same pathologists would like to know if there is a > > standard about wearing gloves in the histo world and if so, is it because > > of squames or although the tissue is fixed, is there chances of infections > > or something else. thank you. > > > > NOTICE TO RECIPIENT: If you are not the intended recipient of this > > e-mail, you are prohibited from sharing, copying, or otherwise using or > > disclosing its contents. If you have received this e-mail in error, > > please notify the sender immediately by reply e-mail and permanently > > delete this e-mail and any attachments without reading, forwarding or > > saving them. Thank you. > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > -- > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BSullivan <@t> virtua.org Thu Mar 21 07:18:13 2013 From: BSullivan <@t> virtua.org (Sullivan, Beatrice) Date: Thu Mar 21 07:19:00 2013 Subject: [Histonet] H&E Stainer Leica vs Sakura (Sophia Lin) In-Reply-To: <77F52EFAB8B1694B885E277C48FCD0F639B5C6F1@GHSEXMBX2W8K1V.geisinger.edu> References: <201303201704.r2KH4UhM024333@mail20c40.carrierzone.com>, <3A581710-3E83-49F4-B325-96DF378BE506@histocare.com> <77F52EFAB8B1694B885E277C48FCD0F639B5C6F1@GHSEXMBX2W8K1V.geisinger.edu> Message-ID: <6932520047F7EE46B512E9801344F160164F073B@EXCHANGEMB-2.Virtua.org> I'd like to use something a little stronger to describe how much I dislike this coverslipper. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bitting, Angela K. Sent: Thursday, March 21, 2013 8:04 AM To: joelle weaver; Contact HistoCare; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] H&E Stainer Leica vs Sakura (Sophia Lin) We hate Leica's CV5030 coverslipper too.!!! "Babysit" is a good word to describe the user experience. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelle weaver Sent: Wednesday, March 20, 2013 7:32 PM To: Contact HistoCare; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] H&E Stainer Leica vs Sakura (Sophia Lin) I agree. The Leica stainer and Sakura are both good instruments, however I really HATE that Leica glass cover slipper and I had the same assessment as to need to "baby sit". I sometimes preferred just hand cover slipping because it was less trouble, and even faster sometimes ( believe that or not). I had none of these issues with the Prisma covcr slipper, and no trouble with the user interface etc. I had the same issue with the door latch on the Prisma, but other than a quick replacement of that, it worked perfectly. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: contact@histocare.com > Date: Wed, 20 Mar 2013 17:49:22 -0500 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] H&E Stainer Leica vs Sakura (Sophia Lin) > > Both stainers are powerhouses. The Leica has a plain menu screen with a simple interface while the Sakura has a LCD screen with detailed information about what stage the staining process a rack is along with multiple menus. The difference between the performance changes drastically when the respective coverslipper attachments become involved. > > The Leica is seriously no match for the Sakura in this respect. The Leica's coverslipper is its Achilles heel and requires a LOT more attention and alerts frequently, very frequently. It takes a separate rack for staining the slides at the beginning of the process and eventually transfers them to a different rack one the cover slip is complete. This one uses glass and frequently drops glass, creates bubbles, drops and breaks slides. You will have to frequently purge the system and clean the cover medium needle dropper. Once done, it only holds. Two racks of 30 slides and will alert until you remove it. You can't leave this one alone for more than 5 minutes without an alert. Seriously. > > The Sakura's coverslipper uses cover tape which won't need to be replaced not even remotely as soon as the glass in the Leica. Finished slides remain in a carousel at the top and can hold about 10 racks of 20 before it alerts. For high volume, the Sakura pair wins hands down. You won't lose productivity time by needing to check on this machine pair. > > HistoCare.com > > > > > > > Hi, > > We are currently looking to switch out our linear MKII stainer for > either a Leica XL autostainer or the Sakura Tissue-Tek Prisma. Any recommendations? > Are quantity of H&Es is increasing and we need adequate equipment to > meet our workload. The incorporated oven seems excellent on both > stainers. Any pros/cons would be greatly appreciated! Also, if you are > currently using the stainer, does it meet your workload and what is your volume? > > Thanks! > > Sophia > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. ------------------------------------------------------------------------- This message was secured by ZixCorp(R). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message, and any included attachments, are from Virtua Health or its related affiliates and is intended only for the addressee(s). The information contained herein is privileged, proprietary or may include confidential information and/or protected patient health information. Any unauthorized review, forwarding, printing, copying, distributing, or otherwise disseminating or taking any action based on such information is strictly prohibited. If you have received this message in error, or have reason to believe you are not authorized to receive it, please delete this message promptly and notify the sender by e-mail with a copy to ISSECURITY@virtua.org. Thank you From dhewitt <@t> hvhs.org Thu Mar 21 07:53:30 2013 From: dhewitt <@t> hvhs.org (Daniel Hewitt) Date: Thu Mar 21 07:53:41 2013 Subject: [Histonet] H&E Stainer Leica vs Sakura (Sophia Lin) In-Reply-To: <6932520047F7EE46B512E9801344F160164F073B@EXCHANGEMB-2.Virtua.org> Message-ID: <7DDB5AB36CBC574D8D680806E7BBE58B0169443F@MX-HVB-02.hvhs.org> I agree a lot of baby sitting with the leica, our other lab has the new Sakura which uses glass instead of tape, they love it. Daniel Hewitt Histology Supervisor, HVS 412-749-7371 This email, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, or an agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and delete and destroy all copies of the original message, including attachments. Please note that any views or opinions presented in this e-mail are solely those of the author and do not necessarily represent those of Heritage Valley Health System. The integrity and security of this message cannot be guaranteed on the internet. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sullivan, Beatrice Sent: Thursday, March 21, 2013 8:18 AM To: Bitting, Angela K.; joelle weaver; Contact HistoCare; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] H&E Stainer Leica vs Sakura (Sophia Lin) I'd like to use something a little stronger to describe how much I dislike this coverslipper. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bitting, Angela K. Sent: Thursday, March 21, 2013 8:04 AM To: joelle weaver; Contact HistoCare; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] H&E Stainer Leica vs Sakura (Sophia Lin) We hate Leica's CV5030 coverslipper too.!!! "Babysit" is a good word to describe the user experience. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelle weaver Sent: Wednesday, March 20, 2013 7:32 PM To: Contact HistoCare; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] H&E Stainer Leica vs Sakura (Sophia Lin) I agree. The Leica stainer and Sakura are both good instruments, however I really HATE that Leica glass cover slipper and I had the same assessment as to need to "baby sit". I sometimes preferred just hand cover slipping because it was less trouble, and even faster sometimes ( believe that or not). I had none of these issues with the Prisma covcr slipper, and no trouble with the user interface etc. I had the same issue with the door latch on the Prisma, but other than a quick replacement of that, it worked perfectly. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: contact@histocare.com > Date: Wed, 20 Mar 2013 17:49:22 -0500 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] H&E Stainer Leica vs Sakura (Sophia Lin) > > Both stainers are powerhouses. The Leica has a plain menu screen with a simple interface while the Sakura has a LCD screen with detailed information about what stage the staining process a rack is along with multiple menus. The difference between the performance changes drastically when the respective coverslipper attachments become involved. > > The Leica is seriously no match for the Sakura in this respect. The Leica's coverslipper is its Achilles heel and requires a LOT more attention and alerts frequently, very frequently. It takes a separate rack for staining the slides at the beginning of the process and eventually transfers them to a different rack one the cover slip is complete. This one uses glass and frequently drops glass, creates bubbles, drops and breaks slides. You will have to frequently purge the system and clean the cover medium needle dropper. Once done, it only holds. Two racks of 30 slides and will alert until you remove it. You can't leave this one alone for more than 5 minutes without an alert. Seriously. > > The Sakura's coverslipper uses cover tape which won't need to be replaced not even remotely as soon as the glass in the Leica. Finished slides remain in a carousel at the top and can hold about 10 racks of 20 before it alerts. For high volume, the Sakura pair wins hands down. You won't lose productivity time by needing to check on this machine pair. > > HistoCare.com > > > > > > > Hi, > > We are currently looking to switch out our linear MKII stainer for > either a Leica XL autostainer or the Sakura Tissue-Tek Prisma. Any recommendations? > Are quantity of H&Es is increasing and we need adequate equipment to > meet our workload. The incorporated oven seems excellent on both > stainers. Any pros/cons would be greatly appreciated! Also, if you are > currently using the stainer, does it meet your workload and what is your volume? > > Thanks! > > Sophia > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. ------------------------------------------------------------------------ - This message was secured by ZixCorp(R). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message, and any included attachments, are from Virtua Health or its related affiliates and is intended only for the addressee(s). The information contained herein is privileged, proprietary or may include confidential information and/or protected patient health information. Any unauthorized review, forwarding, printing, copying, distributing, or otherwise disseminating or taking any action based on such information is strictly prohibited. If you have received this message in error, or have reason to believe you are not authorized to receive it, please delete this message promptly and notify the sender by e-mail with a copy to ISSECURITY@virtua.org. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BZIMMERM <@t> gru.edu Thu Mar 21 08:18:30 2013 From: BZIMMERM <@t> gru.edu (Zimmerman, Billie) Date: Thu Mar 21 08:33:31 2013 Subject: [Histonet] Jekyll Island April 12 - 14, 2013 Vendor Reception and other thoughts... Message-ID: <7B3DEB32E69C034EACB479059C5DE3FF75D245@EX-MLB-03.ad.georgiahealth.edu> The vendor reception will be held Friday, April 12 from 7 - 9pm. We will have acoustic guitar entertainment provided by Jay Sweat. There will be a good selection of music that should please just about everyone. I'm sending everyone the link, in case you might want to dine at the restaurant where we "discovered" Jay Sweat and Travis Thrift. www.seajays.com It's a casual atmosphere and the food is good. Save room for the Key Lime pie. My suggestion is to stay away from the restaurant chains during your visit on Jekyll because you can always eat that stuff when you return home! Here's another restaurant to try: https://foursquare.com/v/fins-on-the-beach/4d99fa59744f370465151458 This restaurant is called Fins on the Beach. I dined on fish tacos. They were very good but I had eaten too many hushpuppies before I was served the entr?e. They serve a basket of hushpuppies with your drink order. Fins has the absolute best view on the island. This restaurant doesn't feel as casual as SeaJays but feel free to wear jeans/shorts. (I don't think I'd wear a speedo or bikini.) ***Last word: Don't visit Jekyll Island and plan on losing weight. You'll be darn lucky if you don't gain a pound or two. Just bring those sweat pants with the elastic waist. Augusta State University and Georgia Health Sciences University have consolidated to become Georgia Regents University. Effective January 9, 2013, my email address has changed to BZIMMERM@gru.edu. Please update your address book to reflect this change. From MICHELLE.LAMPHERE <@t> childrens.com Thu Mar 21 08:18:44 2013 From: MICHELLE.LAMPHERE <@t> childrens.com (Michelle Lamphere) Date: Thu Mar 21 08:39:23 2013 Subject: [Histonet] H&E Stainer Leica vs Sakura (Sophia Lin) Message-ID: I will give the Leica coverslipper some love here....we do not really have any problems. We put the rack in and go do whatever until it is done. I do not get air bubbles and I do not have to babysit. Obviously, as with any piece of equipment, occasionally something goes "out" and we have to have it repaired. But that thing has been chugging along in our lab since 2006. On the other hand, we did have the Sakura tape coverslipper for a couple of years back in the 90's, I think (was before I got here). Almost every one of those slides is un-usable. The tape has warped and pulled off and pulled the tissue sections with it. I am sure they have made improvements since then, but made the lab very leery of using the tape. Michelle M Lamphere, HT (ASCP) Senior Tech, Histology Children's Medical Center 1935 Medical District Drive Dallas, TX 75235 Office :214-456-2798 Histology: 214-456-2318 Fax: 214-456-0779 Please consider the environment before printing this e-mail. This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. From kgrobert <@t> rci.rutgers.edu Thu Mar 21 08:32:09 2013 From: kgrobert <@t> rci.rutgers.edu (kgrobert@rci.rutgers.edu) Date: Thu Mar 21 08:53:29 2013 Subject: [Histonet] H&E Stainer Leica vs Sakura (Sophia Lin) In-Reply-To: References: <201303201704.r2KH4UhM024333@mail20c40.carrierzone.com> <3A581710-3E83-49F4-B325-96DF378BE506@histocare.com> Message-ID: I have the Leica stainer and coverslipper, and I don't have anywhere near as many problems with the coverslipper as described by "Contact" below. Mine alerts once in a while; if his alerts that much, then something is seriously wrong. (The last time mine alerted that much, it needed a new "brain"-this is an older machine that had 5 circuit boards and one gave out-and one new sensor. Still worth it to us to fix it.) Anything as complex as staining and coverslipping robots will be fussy from time to time. But I love my Leica! Kathleen Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology & Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (848) 445-1443 FAX (732) 445-6905 > We have both and love them both. IF you are using tape coverslips then > perhaps Sakura is your best bet. We use glass coverslips on BOTH the Leica > and Sakura and find fewer problems with the Leica. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Contact > HistoCare > Sent: Wednesday, March 20, 2013 6:49 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] H&E Stainer Leica vs Sakura (Sophia Lin) > > Both stainers are powerhouses. The Leica has a plain menu screen with a > simple interface while the Sakura has a LCD screen with detailed > information about what stage the staining process a rack is along with > multiple menus. The difference between the performance changes > drastically when the respective coverslipper attachments become involved. > > The Leica is seriously no match for the Sakura in this respect. The > Leica's coverslipper is its Achilles heel and requires a LOT more > attention and alerts frequently, very frequently. It takes a separate rack > for staining the slides at the beginning of the process and eventually > transfers them to a different rack one the cover slip is complete. This > one uses glass and frequently drops glass, creates bubbles, drops and > breaks slides. You will have to frequently purge the system and clean the > cover medium needle dropper. Once done, it only holds. Two racks of 30 > slides and will alert until you remove it. You can't leave this one alone > for more than 5 minutes without an alert. Seriously. > > The Sakura's coverslipper uses cover tape which won't need to be replaced > not even remotely as soon as the glass in the Leica. Finished slides > remain in a carousel at the top and can hold about 10 racks of 20 before > it alerts. For high volume, the Sakura pair wins hands down. You won't > lose productivity time by needing to check on this machine pair. > > HistoCare.com > > > > > > > Hi, > > We are currently looking to switch out our linear MKII stainer for either > a Leica XL autostainer or the Sakura Tissue-Tek Prisma. Any > recommendations? > Are quantity of H&Es is increasing and we need adequate equipment to meet > our workload. The incorporated oven seems excellent on both stainers. Any > pros/cons would be greatly appreciated! Also, if you are currently using > the stainer, does it meet your workload and what is your volume? > > Thanks! > > Sophia > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From robinsoc <@t> mercyhealth.com Thu Mar 21 09:55:40 2013 From: robinsoc <@t> mercyhealth.com (Cynthia Robinson) Date: Thu Mar 21 09:55:48 2013 Subject: [Histonet] PC control Message-ID: <514AD91C020000AF0000D5D9@nodcdmg2.no.trinity-health.org> I am in need of pneumocystis control. Does anyone have any to share? I have a supply of fungus controls I can trade. Thanks......Cindi Cindi Robinson HT(ASCP) Mercy Medical Center Dunes Medical Laboratories 350 W Anchor Dr Dakota Dunes SD 57049 phone-712-279-2768 robinsoc@mercyhealth.com From turkekul <@t> gmail.com Thu Mar 21 09:58:47 2013 From: turkekul <@t> gmail.com (Mesru T) Date: Thu Mar 21 09:58:50 2013 Subject: [Histonet] Re: Histonet Digest, Vol 112, Issue 22 In-Reply-To: <514b02fe.48a9b60a.5002.2547SMTPIN_ADDED_MISSING@mx.google.com> References: <514b02fe.48a9b60a.5002.2547SMTPIN_ADDED_MISSING@mx.google.com> Message-ID: Dear Histonetters, I am going to do Immunofluoresence staining (beta-catenin, Notch1 and Rnx2) on MMA sections of human iliac crest undecalcified tissue (5um thick). In the literature I have encountered different protocols for removing the MMA. Neil Hand uses warm xylene, Nancy Troiano uses acetone and some other people use the monomer itself. So many options to choose from and to get confused. Does the deplasticizing protocol depends on the MMA embedding method or section thickness? Is there are preferred method of deplasticizing for IHC/IF staining? Another confusion is the antigen retrieval. In some articles antigen retrieval at boiling temperatures has been used, same as in paraffin section IHC. Neil Hand recommends pressure cooker. However, Nancy Troiano uses 50C water bath citric buffer (milder retrieval). Many researchers do not even use antigen retrieval at all. I do not know which is the best method to start optimization. How does the type of antigen retrieval used for IHC depends on the MMA method/polymerization time and temperature used? *Brief MMA protocol:* Activated Methyl Methacrylate (Monomer ) Methyl Methacrylate ???????? 200 ml N-Butyl Pthalate ??????????.. 50 ml Benzoyl Peroxide ????????. .. 8.4 g Store at 4C. *Processing:* ** 70% ethanol ???????????????.5 days Absolute ethanol ????????????.. 2-5 days Xylene ??????????????.... 2-5 days Activated Methyl Methacrylate (Monomer ) ??????????????... 6 days at 4C Polymerize in 34?C oven ????????.. more then 6 days until hard. Regards, Mes From Caroline.Pratt <@t> uphs.upenn.edu Thu Mar 21 09:59:47 2013 From: Caroline.Pratt <@t> uphs.upenn.edu (Pratt, Caroline) Date: Thu Mar 21 09:59:53 2013 Subject: [Histonet] H&E Stainer Leica vs Sakura (Sophia Lin) In-Reply-To: References: <201303201704.r2KH4UhM024333@mail20c40.carrierzone.com><3A581710-3E83-49F4-B325-96DF378BE506@histocare.com> Message-ID: We recently purchased a Sakura and we are very, very pleased with the results and ease of use. :) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kgrobert@rci.rutgers.edu Sent: Thursday, March 21, 2013 9:32 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] H&E Stainer Leica vs Sakura (Sophia Lin) I have the Leica stainer and coverslipper, and I don't have anywhere near as many problems with the coverslipper as described by "Contact" below. Mine alerts once in a while; if his alerts that much, then something is seriously wrong. (The last time mine alerted that much, it needed a new "brain"-this is an older machine that had 5 circuit boards and one gave out-and one new sensor. Still worth it to us to fix it.) Anything as complex as staining and coverslipping robots will be fussy from time to time. But I love my Leica! Kathleen Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology & Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (848) 445-1443 FAX (732) 445-6905 > We have both and love them both. IF you are using tape coverslips then > perhaps Sakura is your best bet. We use glass coverslips on BOTH the Leica > and Sakura and find fewer problems with the Leica. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Contact > HistoCare > Sent: Wednesday, March 20, 2013 6:49 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] H&E Stainer Leica vs Sakura (Sophia Lin) > > Both stainers are powerhouses. The Leica has a plain menu screen with a > simple interface while the Sakura has a LCD screen with detailed > information about what stage the staining process a rack is along with > multiple menus. The difference between the performance changes > drastically when the respective coverslipper attachments become involved. > > The Leica is seriously no match for the Sakura in this respect. The > Leica's coverslipper is its Achilles heel and requires a LOT more > attention and alerts frequently, very frequently. It takes a separate rack > for staining the slides at the beginning of the process and eventually > transfers them to a different rack one the cover slip is complete. This > one uses glass and frequently drops glass, creates bubbles, drops and > breaks slides. You will have to frequently purge the system and clean the > cover medium needle dropper. Once done, it only holds. Two racks of 30 > slides and will alert until you remove it. You can't leave this one alone > for more than 5 minutes without an alert. Seriously. > > The Sakura's coverslipper uses cover tape which won't need to be replaced > not even remotely as soon as the glass in the Leica. Finished slides > remain in a carousel at the top and can hold about 10 racks of 20 before > it alerts. For high volume, the Sakura pair wins hands down. You won't > lose productivity time by needing to check on this machine pair. > > HistoCare.com > > > > > > > Hi, > > We are currently looking to switch out our linear MKII stainer for either > a Leica XL autostainer or the Sakura Tissue-Tek Prisma. Any > recommendations? > Are quantity of H&Es is increasing and we need adequate equipment to meet > our workload. The incorporated oven seems excellent on both stainers. Any > pros/cons would be greatly appreciated! Also, if you are currently using > the stainer, does it meet your workload and what is your volume? > > Thanks! > > Sophia > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From tfountain <@t> dsmanitoba.ca Thu Mar 21 10:53:08 2013 From: tfountain <@t> dsmanitoba.ca (Tiana Baskin) Date: Thu Mar 21 10:53:19 2013 Subject: [Histonet] IHC Stainer Dispense volume Message-ID: I currently use a Dako Autostainer and we are dispensing 300uL of all reagents to the slide(100uL per drop zone). I am just curious what others are dispensing and if you've noticed any difference by changing the dispense volume in regards to stain quality. Does anyone adjust for very large tissue sections (that cover the majority of the slide)? Thanks :) -------------- next part -------------- This email and/or any documents in this transmission is intended for the addressee(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original. Ce courriel et tout document dans cette transmission est destin? ? la personne ou aux personnes ? qui il est adress?. Il peut contenir des informations privil?gi?es ou confidentielles. Toute utilisation, divulgation, distribution, copie, ou diffusion non autoris?e est strictement d?fendue. Si vous n'?tes pas le destinataire de ce message, veuillez en informer l'exp?diteur imm?diatement et lui remettre l'original. From Kim <@t> ncpath.com Thu Mar 21 11:27:41 2013 From: Kim <@t> ncpath.com (Kimmie Rabe) Date: Thu Mar 21 11:27:51 2013 Subject: [Histonet] ventana counterstaining Message-ID: Has anyone else had problems with the counterstain on the ventana being pink instead of that lovely pale blue color? Ventana says it's because we use Sta-On, but we don't always use Sta-On and the pink vs. blue staining doesn't correlate with whether Sta-On has been used or not. Don't get me wrong, I have nothing against pink. But if there is some problem with the hematoxylin we purchase from ventana or the dispensing or mixing thereof--we would like to know. Thanks! Kimmie E. Rabe, MD North Central Pathology, PA 3701 12th Street North, Suite 201 St. Cloud, MN 56303 Phone: 320-253-6554 Fax: 320-253-1218 kim@ncpath.com From LSebree <@t> uwhealth.org Thu Mar 21 11:41:21 2013 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Thu Mar 21 11:41:29 2013 Subject: [Histonet] RE: ventana counterstaining In-Reply-To: References: Message-ID: <77DD817201982748BC67D7960F2F76AF03E5B5@UWHC-MBX12.uwhis.hosp.wisc.edu> Kimmie, We use VMS' Hematoxylin II with no changes from how it has always stained. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kimmie Rabe Sent: Thursday, March 21, 2013 11:28 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] ventana counterstaining Has anyone else had problems with the counterstain on the ventana being pink instead of that lovely pale blue color? Ventana says it's because we use Sta-On, but we don't always use Sta-On and the pink vs. blue staining doesn't correlate with whether Sta-On has been used or not. Don't get me wrong, I have nothing against pink. But if there is some problem with the hematoxylin we purchase from ventana or the dispensing or mixing thereof--we would like to know. Thanks! Kimmie E. Rabe, MD North Central Pathology, PA 3701 12th Street North, Suite 201 St. Cloud, MN 56303 Phone: 320-253-6554 Fax: 320-253-1218 kim@ncpath.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BGapinski <@t> pathgroup.com Thu Mar 21 12:30:05 2013 From: BGapinski <@t> pathgroup.com (Bruce Gapinski) Date: Thu Mar 21 12:30:24 2013 Subject: [Histonet] Leica CV5030 Message-ID: This coverslipper is over engineered. No self respecting histologist would design something that holds the slide a foot in the air by two "v" shaped grippers. Gravity sucks and the slide falls. I have a box of DISTROYED slides that I show the repairman. Can you imagine "Sorry Mr. Smith we can't read your biopsy because it splintered into pieces AFTER we did all the hard work." "Good luck with that health of yours." It is NOT compatible with Ventana labels, as the gum sticks to those grippers and the slide falls, or jams in the output rack. To tell you the truth, I loved the old Leica coverslipper. It had a "walking beam" and never broke a slide. I'm in the mood for a Sakaura. Bruce Gapinsk HT (ASCP) Chief Histologist Marin Medical Laboratories PathGroup SF ________________________________ Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you From mroark <@t> sfmc.net Thu Mar 21 12:31:55 2013 From: mroark <@t> sfmc.net (Matthew Roark) Date: Thu Mar 21 12:32:30 2013 Subject: [Histonet] H&E Stainer Leica vs Sakura (Sophia Lin) In-Reply-To: References: <201303201704.r2KH4UhM024333@mail20c40.carrierzone.com><3A581710-3E83-49F4-B325-96DF378BE506@histocare.com> Message-ID: <004401ce2659$fb833d30$f289b790$@net> I currently use the Leica stainer and coverslipper without any problems. I took some time, at first, to fine tune the coverslipper but have not had any real problems since. Both are workhorses. Matthew Roark- HT/HTL(ASCP)CM Histology Specialist Saint Francis Medical Center 211 Saint Francis Drive Cape Girardeau, MO 63703 573-331-5267 mroark@sfmc.net http://www.sfmc.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pratt, Caroline Sent: Thursday, March 21, 2013 10:00 AM To: kgrobert@rci.rutgers.edu; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] H&E Stainer Leica vs Sakura (Sophia Lin) We recently purchased a Sakura and we are very, very pleased with the results and ease of use. :) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kgrobert@rci.rutgers.edu Sent: Thursday, March 21, 2013 9:32 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] H&E Stainer Leica vs Sakura (Sophia Lin) I have the Leica stainer and coverslipper, and I don't have anywhere near as many problems with the coverslipper as described by "Contact" below. Mine alerts once in a while; if his alerts that much, then something is seriously wrong. (The last time mine alerted that much, it needed a new "brain"-this is an older machine that had 5 circuit boards and one gave out-and one new sensor. Still worth it to us to fix it.) Anything as complex as staining and coverslipping robots will be fussy from time to time. But I love my Leica! Kathleen Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology & Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (848) 445-1443 FAX (732) 445-6905 > We have both and love them both. IF you are using tape coverslips then > perhaps Sakura is your best bet. We use glass coverslips on BOTH the Leica > and Sakura and find fewer problems with the Leica. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Contact > HistoCare > Sent: Wednesday, March 20, 2013 6:49 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] H&E Stainer Leica vs Sakura (Sophia Lin) > > Both stainers are powerhouses. The Leica has a plain menu screen with a > simple interface while the Sakura has a LCD screen with detailed > information about what stage the staining process a rack is along with > multiple menus. The difference between the performance changes > drastically when the respective coverslipper attachments become involved. > > The Leica is seriously no match for the Sakura in this respect. The > Leica's coverslipper is its Achilles heel and requires a LOT more > attention and alerts frequently, very frequently. It takes a separate rack > for staining the slides at the beginning of the process and eventually > transfers them to a different rack one the cover slip is complete. This > one uses glass and frequently drops glass, creates bubbles, drops and > breaks slides. You will have to frequently purge the system and clean the > cover medium needle dropper. Once done, it only holds. Two racks of 30 > slides and will alert until you remove it. You can't leave this one alone > for more than 5 minutes without an alert. Seriously. > > The Sakura's coverslipper uses cover tape which won't need to be replaced > not even remotely as soon as the glass in the Leica. Finished slides > remain in a carousel at the top and can hold about 10 racks of 20 before > it alerts. For high volume, the Sakura pair wins hands down. You won't > lose productivity time by needing to check on this machine pair. > > HistoCare.com > > > > > > > Hi, > > We are currently looking to switch out our linear MKII stainer for either > a Leica XL autostainer or the Sakura Tissue-Tek Prisma. Any > recommendations? > Are quantity of H&Es is increasing and we need adequate equipment to meet > our workload. The incorporated oven seems excellent on both stainers. Any > pros/cons would be greatly appreciated! Also, if you are currently using > the stainer, does it meet your workload and what is your volume? > > Thanks! > > Sophia > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Mike.Beske <@t> ministryhealth.org Thu Mar 21 13:08:54 2013 From: Mike.Beske <@t> ministryhealth.org (Beske, Mike) Date: Thu Mar 21 13:09:00 2013 Subject: [Histonet] Leica IP slide printer Message-ID: <66A6045667D5BF42816FCA774714444A06CFB9E11D@EXMHCMBX01VS.ministryhealth.net> Does anyone have a Leica IP slide printer that interfaces with Meditech Magic workstation and if you do, how is it working for you? Mike Beske, Pathology, St. Mary's Hospital, Rhinelander, Wi ________________________________ CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. From kmilne <@t> bccancer.bc.ca Thu Mar 21 13:30:57 2013 From: kmilne <@t> bccancer.bc.ca (Milne, Katy) Date: Thu Mar 21 13:31:26 2013 Subject: [Histonet] IHC Stainer Dispense volume In-Reply-To: <1b0eace5-b834-49d3-aa33-ef2beefe2fcd@SRVEXHT01.phsabc.ehcnet.ca> Message-ID: <3FEFF18FF4E1914A9AB7D8498591BE86012371AE1604@VEXCCR02.phsabc.ehcnet.ca> Hi Tiana, We just got a Biocare Intellipath FLX and it came set at 300ul too. I've switched it to 150ul, dropping in the middle zone only and had no problems, may see if I can go 100ul for smaller tissues, will up it for larger tissues. I pap pen around the samples though to make sure that reagent doesn't wander off. You may want to find a scrap piece of tissue and an antibody to a marker that should be expressed all though the tissue, right to the edges as much as you can; use a lower drop volume and make sure you get staining all the way across your tissue. Then be consistent about sample placement on the slide and pap-penning and you'll probably be fine, if your system is similar to mine that is! :) Katy From jqb7 <@t> cdc.gov Thu Mar 21 13:48:52 2013 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/OID/NCEZID)) Date: Thu Mar 21 13:49:11 2013 Subject: [Histonet] H&E Stainer Leica vs Sakura (Sophia Lin) In-Reply-To: <6932520047F7EE46B512E9801344F160164F073B@EXCHANGEMB-2.Virtua.org> References: <201303201704.r2KH4UhM024333@mail20c40.carrierzone.com>, <3A581710-3E83-49F4-B325-96DF378BE506@histocare.com> <77F52EFAB8B1694B885E277C48FCD0F639B5C6F1@GHSEXMBX2W8K1V.geisinger.edu> <6932520047F7EE46B512E9801344F160164F073B@EXCHANGEMB-2.Virtua.org> Message-ID: I love it. For a glass coverslipper............................. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sullivan, Beatrice Sent: Thursday, March 21, 2013 8:18 AM To: Bitting, Angela K.; joelle weaver; Contact HistoCare; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] H&E Stainer Leica vs Sakura (Sophia Lin) I'd like to use something a little stronger to describe how much I dislike this coverslipper. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bitting, Angela K. Sent: Thursday, March 21, 2013 8:04 AM To: joelle weaver; Contact HistoCare; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] H&E Stainer Leica vs Sakura (Sophia Lin) We hate Leica's CV5030 coverslipper too.!!! "Babysit" is a good word to describe the user experience. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelle weaver Sent: Wednesday, March 20, 2013 7:32 PM To: Contact HistoCare; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] H&E Stainer Leica vs Sakura (Sophia Lin) I agree. The Leica stainer and Sakura are both good instruments, however I really HATE that Leica glass cover slipper and I had the same assessment as to need to "baby sit". I sometimes preferred just hand cover slipping because it was less trouble, and even faster sometimes ( believe that or not). I had none of these issues with the Prisma covcr slipper, and no trouble with the user interface etc. I had the same issue with the door latch on the Prisma, but other than a quick replacement of that, it worked perfectly. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: contact@histocare.com > Date: Wed, 20 Mar 2013 17:49:22 -0500 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] H&E Stainer Leica vs Sakura (Sophia Lin) > > Both stainers are powerhouses. The Leica has a plain menu screen with a simple interface while the Sakura has a LCD screen with detailed information about what stage the staining process a rack is along with multiple menus. The difference between the performance changes drastically when the respective coverslipper attachments become involved. > > The Leica is seriously no match for the Sakura in this respect. The Leica's coverslipper is its Achilles heel and requires a LOT more attention and alerts frequently, very frequently. It takes a separate rack for staining the slides at the beginning of the process and eventually transfers them to a different rack one the cover slip is complete. This one uses glass and frequently drops glass, creates bubbles, drops and breaks slides. You will have to frequently purge the system and clean the cover medium needle dropper. Once done, it only holds. Two racks of 30 slides and will alert until you remove it. You can't leave this one alone for more than 5 minutes without an alert. Seriously. > > The Sakura's coverslipper uses cover tape which won't need to be replaced not even remotely as soon as the glass in the Leica. Finished slides remain in a carousel at the top and can hold about 10 racks of 20 before it alerts. For high volume, the Sakura pair wins hands down. You won't lose productivity time by needing to check on this machine pair. > > HistoCare.com > > > > > > > Hi, > > We are currently looking to switch out our linear MKII stainer for > either a Leica XL autostainer or the Sakura Tissue-Tek Prisma. Any recommendations? > Are quantity of H&Es is increasing and we need adequate equipment to > meet our workload. The incorporated oven seems excellent on both > stainers. Any pros/cons would be greatly appreciated! Also, if you are > currently using the stainer, does it meet your workload and what is your volume? > > Thanks! > > Sophia > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. ------------------------------------------------------------------------- This message was secured by ZixCorp(R). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message, and any included attachments, are from Virtua Health or its related affiliates and is intended only for the addressee(s). The information contained herein is privileged, proprietary or may include confidential information and/or protected patient health information. Any unauthorized review, forwarding, printing, copying, distributing, or otherwise disseminating or taking any action based on such information is strictly prohibited. If you have received this message in error, or have reason to believe you are not authorized to receive it, please delete this message promptly and notify the sender by e-mail with a copy to ISSECURITY@virtua.org. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b-frederick <@t> northwestern.edu Thu Mar 21 14:04:03 2013 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Thu Mar 21 14:04:09 2013 Subject: [Histonet] H&E Stainer Leica vs Sakura (Sophia Lin) In-Reply-To: References: <201303201704.r2KH4UhM024333@mail20c40.carrierzone.com>, <3A581710-3E83-49F4-B325-96DF378BE506@histocare.com> <77F52EFAB8B1694B885E277C48FCD0F639B5C6F1@GHSEXMBX2W8K1V.geisinger.edu> <6932520047F7EE46B512E9801344F160164F073B@EXCHANGEMB-2.Virtua.org> Message-ID: <62C639732D3F274DACED033EBDF6ADAF20D1157F@evcspmbx2.ads.northwestern.edu> We only babysit when we receive outside slides or the label was not removed prior to staining. It can be adjusted to fit the parameters of the slides used. We have the XL and connected coverslipper. Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/OID/NCEZID) Sent: Thursday, March 21, 2013 1:49 PM To: 'Sullivan, Beatrice'; Bitting, Angela K.; joelle weaver; Contact HistoCare; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] H&E Stainer Leica vs Sakura (Sophia Lin) I love it. For a glass coverslipper............................. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sullivan, Beatrice Sent: Thursday, March 21, 2013 8:18 AM To: Bitting, Angela K.; joelle weaver; Contact HistoCare; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] H&E Stainer Leica vs Sakura (Sophia Lin) I'd like to use something a little stronger to describe how much I dislike this coverslipper. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bitting, Angela K. Sent: Thursday, March 21, 2013 8:04 AM To: joelle weaver; Contact HistoCare; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] H&E Stainer Leica vs Sakura (Sophia Lin) We hate Leica's CV5030 coverslipper too.!!! "Babysit" is a good word to describe the user experience. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelle weaver Sent: Wednesday, March 20, 2013 7:32 PM To: Contact HistoCare; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] H&E Stainer Leica vs Sakura (Sophia Lin) I agree. The Leica stainer and Sakura are both good instruments, however I really HATE that Leica glass cover slipper and I had the same assessment as to need to "baby sit". I sometimes preferred just hand cover slipping because it was less trouble, and even faster sometimes ( believe that or not). I had none of these issues with the Prisma covcr slipper, and no trouble with the user interface etc. I had the same issue with the door latch on the Prisma, but other than a quick replacement of that, it worked perfectly. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: contact@histocare.com > Date: Wed, 20 Mar 2013 17:49:22 -0500 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] H&E Stainer Leica vs Sakura (Sophia Lin) > > Both stainers are powerhouses. The Leica has a plain menu screen with a simple interface while the Sakura has a LCD screen with detailed information about what stage the staining process a rack is along with multiple menus. The difference between the performance changes drastically when the respective coverslipper attachments become involved. > > The Leica is seriously no match for the Sakura in this respect. The Leica's coverslipper is its Achilles heel and requires a LOT more attention and alerts frequently, very frequently. It takes a separate rack for staining the slides at the beginning of the process and eventually transfers them to a different rack one the cover slip is complete. This one uses glass and frequently drops glass, creates bubbles, drops and breaks slides. You will have to frequently purge the system and clean the cover medium needle dropper. Once done, it only holds. Two racks of 30 slides and will alert until you remove it. You can't leave this one alone for more than 5 minutes without an alert. Seriously. > > The Sakura's coverslipper uses cover tape which won't need to be replaced not even remotely as soon as the glass in the Leica. Finished slides remain in a carousel at the top and can hold about 10 racks of 20 before it alerts. For high volume, the Sakura pair wins hands down. You won't lose productivity time by needing to check on this machine pair. > > HistoCare.com > > > > > > > Hi, > > We are currently looking to switch out our linear MKII stainer for > either a Leica XL autostainer or the Sakura Tissue-Tek Prisma. Any recommendations? > Are quantity of H&Es is increasing and we need adequate equipment to > meet our workload. The incorporated oven seems excellent on both > stainers. Any pros/cons would be greatly appreciated! Also, if you are > currently using the stainer, does it meet your workload and what is your volume? > > Thanks! > > Sophia > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. ------------------------------------------------------------------------- This message was secured by ZixCorp(R). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message, and any included attachments, are from Virtua Health or its related affiliates and is intended only for the addressee(s). The information contained herein is privileged, proprietary or may include confidential information and/or protected patient health information. Any unauthorized review, forwarding, printing, copying, distributing, or otherwise disseminating or taking any action based on such information is strictly prohibited. If you have received this message in error, or have reason to believe you are not authorized to receive it, please delete this message promptly and notify the sender by e-mail with a copy to ISSECURITY@virtua.org. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HornHV <@t> archildrens.org Thu Mar 21 14:26:26 2013 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Thu Mar 21 14:26:32 2013 Subject: [Histonet] RE: Leica CV5030 In-Reply-To: References: Message-ID: <25A4DE08332B19499904459F00AAACB719BE1356B0@EVS1.archildrens.org> We love our Leica CV5030. It rarely breaks a slide. It works well with our Leica Bond labels. I would buy another one. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bruce Gapinski Sent: Thursday, March 21, 2013 12:30 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Leica CV5030 This coverslipper is over engineered. No self respecting histologist would design something that holds the slide a foot in the air by two "v" shaped grippers. Gravity sucks and the slide falls. I have a box of DISTROYED slides that I show the repairman. Can you imagine "Sorry Mr. Smith we can't read your biopsy because it splintered into pieces AFTER we did all the hard work." "Good luck with that health of yours." It is NOT compatible with Ventana labels, as the gum sticks to those grippers and the slide falls, or jams in the output rack. To tell you the truth, I loved the old Leica coverslipper. It had a "walking beam" and never broke a slide. I'm in the mood for a Sakaura. Bruce Gapinsk HT (ASCP) Chief Histologist Marin Medical Laboratories PathGroup SF ________________________________ Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From mlunetta <@t> luhcares.org Thu Mar 21 14:31:38 2013 From: mlunetta <@t> luhcares.org (Matthew Lunetta) Date: Thu Mar 21 14:31:43 2013 Subject: [Histonet] Colorado State Histo Meeting April 19-20 Message-ID: <170CACC1CA297947A168665B532DD05C21ADCC8C@EXLUH01.Ad.luhcares.org> Hey Netters, It is not to late to join us in Fort Collins. Go to http://www.coloradohisto.org/ to register and check out the program. Respectfully, Matt Lunetta BS HT(ASCP) Lead Histology Longmont United Hospital From trathborne <@t> somerset-healthcare.com Thu Mar 21 14:31:35 2013 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Thu Mar 21 14:32:22 2013 Subject: [Histonet] RE: Leica CV5030 In-Reply-To: <25A4DE08332B19499904459F00AAACB719BE1356B0@EVS1.archildrens.org> References: <25A4DE08332B19499904459F00AAACB719BE1356B0@EVS1.archildrens.org> Message-ID: <3AD061FE740D464FAC7BF6B5CFB757076C660AB9@smcmail02.somerset-healthcare.com> So would I. Once the initial quirks were worked out, it rarely has a problem. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Thursday, March 21, 2013 3:26 PM To: 'Bruce Gapinski'; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Leica CV5030 We love our Leica CV5030. It rarely breaks a slide. It works well with our Leica Bond labels. I would buy another one. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bruce Gapinski Sent: Thursday, March 21, 2013 12:30 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Leica CV5030 This coverslipper is over engineered. No self respecting histologist would design something that holds the slide a foot in the air by two "v" shaped grippers. Gravity sucks and the slide falls. I have a box of DISTROYED slides that I show the repairman. Can you imagine "Sorry Mr. Smith we can't read your biopsy because it splintered into pieces AFTER we did all the hard work." "Good luck with that health of yours." It is NOT compatible with Ventana labels, as the gum sticks to those grippers and the slide falls, or jams in the output rack. To tell you the truth, I loved the old Leica coverslipper. It had a "walking beam" and never broke a slide. I'm in the mood for a Sakaura. Bruce Gapinsk HT (ASCP) Chief Histologist Marin Medical Laboratories PathGroup SF ________________________________ Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Margaret.Perry <@t> sdstate.edu Thu Mar 21 15:02:14 2013 From: Margaret.Perry <@t> sdstate.edu (Perry, Margaret) Date: Thu Mar 21 15:02:21 2013 Subject: [Histonet] polymer detection Message-ID: <25F4FBA34BE9D142964ECC4525B82AEE956D9E9B@SDSU-EX02.jacks.local> I would like to get your opinion about rabbit/mouse universal polymer detection systems. Are there any out there that have little or no background when used on canine ,feline, ovine, bovine, porcine, equine or caprine tissues? Right now I use separate polymers for rabbit or mouse antibodies. We use a DAKO autostainer. I'm just trying to get a feel about what is new to the field of polymers. Thank you for your replies. Margaret Perry HT(ASCP) Veterinary & Biomedical Sciences Department North Campus Drive Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 From liz <@t> premierlab.com Thu Mar 21 15:10:21 2013 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Thu Mar 21 15:10:25 2013 Subject: [Histonet] RE: polymer detection In-Reply-To: <25F4FBA34BE9D142964ECC4525B82AEE956D9E9B@SDSU-EX02.jacks.local> References: <25F4FBA34BE9D142964ECC4525B82AEE956D9E9B@SDSU-EX02.jacks.local> Message-ID: <14E2C6176416974295479C64A11CB9AE016411AC28F1@SBS2K8.premierlab.local> Margaret We personally do not use dual link reagents when working on animal tissue. We have found some issues with mouse polymers and porcine tissue in the past. I know the dual links make things a bit simpler but we prefer not to use them at all. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Laboratory Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 Work (303) 682-3949 Fax (303) 682-9060 Cell (303) 881-0763 liz@premierlab.com www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Perry, Margaret Sent: Thursday, March 21, 2013 2:02 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] polymer detection I would like to get your opinion about rabbit/mouse universal polymer detection systems. Are there any out there that have little or no background when used on canine ,feline, ovine, bovine, porcine, equine or caprine tissues? Right now I use separate polymers for rabbit or mouse antibodies. We use a DAKO autostainer. I'm just trying to get a feel about what is new to the field of polymers. Thank you for your replies. Margaret Perry HT(ASCP) Veterinary & Biomedical Sciences Department North Campus Drive Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carl.hobbs <@t> kcl.ac.uk Thu Mar 21 15:41:09 2013 From: carl.hobbs <@t> kcl.ac.uk (Hobbs, Carl) Date: Thu Mar 21 15:41:28 2013 Subject: [Histonet] Immunofluorescence on FFPE skin Message-ID: <00D6B8253EAED840B8D04E235497381822D7C922@AM2PRD0311MB399.eurprd03.prod.outlook.com> To me, the critical data is: which Abs do you want to use on FFPWS of skin, please? If you do chromogen IHC on your skins already, with them Abs, you can do IF. Sure, autofluorescence can be a problem but......not that big a problem. Interestedly, Carl NB: that article is......strange. Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD School of Biomedical Sciences Kings College London Guys Campus SE1 1UL Tel: 020 78486813 Fax: 020 78486816 020 78486813 From pruegg <@t> ihctech.net Thu Mar 21 16:58:00 2013 From: pruegg <@t> ihctech.net (pruegg@ihctech.net) Date: Thu Mar 21 16:58:04 2013 Subject: [Histonet] Mallory triple stain Message-ID: <005001ce267f$27fcbce0$77f636a0$@ihctech.net> Does anyone know what this is about? I think these people just want a trichrome stain and we are already doing a Masson's TC for them using aniline blue. I think they are just asking for a modified version of TC and want to make sure we use aniline blue for the collagen stain??? Is Mallory Triple stain something other than a modified trichrome stain? "What we were asking for was Mallory Triple stain, which appears to be the synonymous with Mallory Aniline Blue, and Mallory Trichrome. Is that correct, that those three names are for the same staining technique? The main components of the stain that we were looking for were: aniline blue, orange G, and acid fuchsin. As far as I can tell all three techniques have those three components." Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E. Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 rueggihcconsultingpr@outlook.com This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. From YuJ2 <@t> upmc.edu Thu Mar 21 18:26:47 2013 From: YuJ2 <@t> upmc.edu (Yu, Jian) Date: Thu Mar 21 18:26:53 2013 Subject: [Histonet] Mouse bone blocks/sections, bone marrow smears In-Reply-To: <7E0A77BFEB9A1E47A63F978E7116821F02AEC667@1upmc-msx11.acct.upmchs.net> References: <471953BC63077941B82C26A4338272B42F04E0@ORLEV03.hca.corpad.net> <7E0A77BFEB9A1E47A63F978E7116821F02AEC667@1upmc-msx11.acct.upmchs.net> Message-ID: <9B948E3AC1EA244FA291AEB778ECDCD02C906D@MSXMBXNSPRD02.acct.upmchs.net> Dear all, We like to make paraffin embedded bone of mouse tibia and cut longitudinal sections for H&E sections. Does anyone have a easy-to-follow protocol to prepare (fix and decal) the bone before processing? We routinely fix soft tissue (ie. mouse GI tract and liver) and send off to our pathology core for processing and embedding. Any tips on cutting the blocks is helpful too. These sections appear brittle. We are also interested in a practical protocol for making mouse BM smears and staining to determine cellularity changes after radiation. We just started working with the bone. Any advice will be very helpful and greatly appreciated. Thanks, Jian ******************************************************************* Jian Yu, Ph.D. University of Pittsburgh Cancer Institute Email: yuj2@upmc.edu ******************************************************************* From Mark.Elliott <@t> hli.ubc.ca Thu Mar 21 22:24:16 2013 From: Mark.Elliott <@t> hli.ubc.ca (Mark Elliott) Date: Thu Mar 21 22:24:30 2013 Subject: [Histonet] Dispenser volumes In-Reply-To: References: Message-ID: <514B6C70020000D600098240@mail.hli.ubc.ca> I too use the DAKO Autostainer and have for many years. My default setting is 100 ul and drop zone in the middle of the slide, however, I adjust the volume and the drop zone depending on how large the section is and where the section is on the slide in order to conserve reagents.I don't notice any difference in staining. Mark UBC-James Hogg Research Centre Vancouver BC Message: 7 Date: Thu, 21 Mar 2013 15:53:08 +0000 From: Tiana Baskin Subject: [Histonet] IHC Stainer Dispense volume To: "'histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain; charset="us-ascii" I currently use a Dako Autostainer and we are dispensing 300uL of all reagents to the slide(100uL per drop zone). I am just curious what others are dispensing and if you've noticed any difference by changing the dispense volume in regards to stain quality. Does anyone adjust for very large tissue sections (that cover the majority of the slide)? ***CONFIDENTIALITY NOTICE*** This electronic message and any attachments are intended only for the use of the addressee and may contain information that is privileged and confidential. Any dissemination, distribution or copying of this communication by unauthorized individuals is strictly prohibited. If you have received this communication in error, please notify the sender immediately by reply e-mail and delete the original and all copies from your system. From mikael.niku <@t> helsinki.fi Fri Mar 22 05:59:28 2013 From: mikael.niku <@t> helsinki.fi (Mikael Niku) Date: Fri Mar 22 06:01:43 2013 Subject: [Histonet] Cheap digital camera microscope adapters? Message-ID: <514C3990.1040704@helsinki.fi> Hello! I'm planning to purchase a set of digital cameras for our histology teaching laboratory, so that the students can do their histology work by taking microphotographs. Buying "real" microscope cameras would be too expensive as we need quite many (and our course microscopes don't have a camera tubus) so I'm thinking about fitting standard digital cameras in place of the eyepiece. The microscopes are good old Olympus CH2 ones and the cameras could be any affordable model. If anyone can recommend a functional but affordable adapter, fitting to a functional but affordable consumer level digital camera, please tell! With best regards, Mikael Niku, PhD University of Helsinki, Finland From rjbuesa <@t> yahoo.com Fri Mar 22 08:24:25 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Mar 22 08:24:30 2013 Subject: [Histonet] Mallory triple stain In-Reply-To: <005001ce267f$27fcbce0$77f636a0$@ihctech.net> References: <005001ce267f$27fcbce0$77f636a0$@ihctech.net> Message-ID: <1363958665.76274.YahooMailNeo@web163104.mail.bf1.yahoo.com> Not really! There are scores of trichrome stains and Mallory is one of them with the characteristic that cell nuclei are not stained with hematoxylin, while in Masson TC they are. I personally prefer Mallory, it is more color "rich" and simpler to do. It was invented at the end of the XIX century and?Masson's in the early 1930's With TC stains the histologist's preferences rule, even when all stain the same but with different colors and intensities. Ren? J. From: "pruegg@ihctech.net" To: histonet@lists.utsouthwestern.edu Sent: Thursday, March 21, 2013 5:58 PM Subject: [Histonet] Mallory triple stain Does anyone know what this is about?? I think these people just want a trichrome stain and we are already doing a Masson's TC for them using aniline blue.? I think they are just asking for a modified version of TC and want to make sure we use aniline blue for the collagen stain???? Is Mallory Triple stain something other than a modified trichrome stain? "What we were asking for was Mallory Triple stain, which appears to be the synonymous with Mallory Aniline Blue, and Mallory Trichrome. Is that correct, that those three names are for the same staining technique? The main components of the stain that we were looking for were: aniline blue, orange G, and acid fuchsin. As far as I can tell all three techniques have those three components." Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E. Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 rueggihcconsultingpr@outlook.com This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Mar 22 08:50:19 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Mar 22 08:51:10 2013 Subject: [Histonet] Cheap digital camera microscope adapters? In-Reply-To: <514C3990.1040704@helsinki.fi> References: <514C3990.1040704@helsinki.fi> Message-ID: <1363960219.88345.YahooMailNeo@web163105.mail.bf1.yahoo.com> If you have a Nikon Coolpix, you can adapt it to the microscope very easily although the Nikon Coolpix is not cheap. There is an article in "Histologic" [34(2):27-32 (2001)] that will give you good ideas. If you go to http://www.wpiinc.com/ there is an assortment of cheap "image capturing" devices of relatively good quality at low price. You can find image capturing devices at http://www.drinstruments.com/ for less than $100, but the images quality are wanting.? At http://www.srb-griturn.com/ they sell camera adapters that you can use to hold a camera to the ocular tube?to take photomicrographs of acceptable quality. Ren? J.? From: Mikael Niku To: histonet@lists.utsouthwestern.edu Sent: Friday, March 22, 2013 6:59 AM Subject: [Histonet] Cheap digital camera microscope adapters? Hello! I'm planning to purchase a set of digital cameras for our histology teaching laboratory, so that the students can do their histology work by taking microphotographs. Buying "real" microscope cameras would be too expensive as we need quite many (and our course microscopes don't have a camera tubus) so I'm thinking about fitting standard digital cameras in place of the eyepiece. The microscopes are good old Olympus CH2 ones and the cameras could be any affordable model. If anyone can recommend a functional but affordable adapter, fitting to a functional but affordable consumer level digital camera, please tell! With best regards, Mikael Niku, PhD University of Helsinki, Finland _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ADuddey <@t> firsthealth.org Fri Mar 22 09:03:03 2013 From: ADuddey <@t> firsthealth.org (Duddey, Aimee) Date: Fri Mar 22 09:03:16 2013 Subject: [Histonet] Georgia Society for Histology---IT'S NOT TOO LATE!!! What cha' gonna do at Jekyll?? In-Reply-To: <7B3DEB32E69C034EACB479059C5DE3FF75C0B7@EX-MLB-03.ad.georgiahealth.edu> References: <7B3DEB32E69C034EACB479059C5DE3FF75C0B7@EX-MLB-03.ad.georgiahealth.edu> Message-ID: Can you please email me the information for your GSH meeting? I think I might like to attend. aduddey@firsthealth.org Aimee -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Zimmerman, Billie Sent: Monday, March 18, 2013 9:28 AM To: histonet@lists.utsouthwestern.edu Cc: Wanda Simons Subject: [Histonet] Georgia Society for Histology---IT'S NOT TOO LATE!!! What cha' gonna do at Jekyll?? Room rates remain at the symposium price IF AVAILABLE. (all inclusive price is $135) If anyone happens to say they are bored at the GSH meeting...I just wouldn't understand. Here's a mini list : * Sunrises and Sunsets (personally I've never seen a sunrise at the beach, what's the point, you can see the same thing on the other side in the evening, I'm not a morning person but maybe I will put a sunrise on my bucket list.) Don't tell Wanda I said this! * Turtle center * Kayaking and fishing * Horseback riding on the beach * Bicycle trails * Historic golf course * Tennis center * Historic Jekyll island club and village * High Tea at the Jekyll Club * Dolphin tours * Karaoke in the Sandbar Lounge * Putt putt golf * Fresh seafood * Brunswick stew( originated in the area) Original version had squirrel and/or rabbit EEEWWWEEE!!! Augusta State University and Georgia Health Sciences University have consolidated to become Georgia Regents University. Effective January 9, 2013, my email address has changed to BZIMMERM@gru.edu. Please update your address book to reflect this change. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From smcbride <@t> andrew.cmu.edu Fri Mar 22 09:10:40 2013 From: smcbride <@t> andrew.cmu.edu (Sean McBride) Date: Fri Mar 22 09:10:45 2013 Subject: [Histonet] Mouse bone blocks/sections, bone marrow smears In-Reply-To: <9B948E3AC1EA244FA291AEB778ECDCD02C906D@MSXMBXNSPRD02.acct.upmchs.net> References: <471953BC63077941B82C26A4338272B42F04E0@ORLEV03.hca.corpad.net> <7E0A77BFEB9A1E47A63F978E7116821F02AEC667@1upmc-msx11.acct.upmchs.net> <9B948E3AC1EA244FA291AEB778ECDCD02C906D@MSXMBXNSPRD02.acct.upmchs.net> Message-ID: Hi Jian, I specialize in orthopedic histology, and I also live in Pittsburgh. I would be glad to discuss the details of your project and see if I can be of any assistance to you. Please contact me at your convenience. Best regards, ~Sean McBride Scientific Specialist Bone Tissue Engineering Center Carnegie Mellon Research Institute 700 Technology Drive Pittsburgh, PA 15219-3124 571-989-BONE (m) 412-268-8275 (fax) seanmcbride007@me.com On Mar 21, 2013, at 7:26 PM, Yu, Jian wrote: > Dear all, > > We like to make paraffin embedded bone of mouse tibia and cut longitudinal sections for H&E sections. > > Does anyone have a easy-to-follow protocol to prepare (fix and decal) the bone before processing? We routinely fix soft tissue (ie. mouse GI tract and liver) and send off to our pathology core for processing and embedding. > > Any tips on cutting the blocks is helpful too. These sections appear brittle. > > We are also interested in a practical protocol for making mouse BM smears and staining to determine cellularity changes after radiation. > > We just started working with the bone. Any advice will be very helpful and greatly appreciated. > > Thanks, Jian > ******************************************************************* > Jian Yu, Ph.D. > University of Pittsburgh Cancer Institute > Email: yuj2@upmc.edu > ******************************************************************* > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From contact <@t> histocare.com Fri Mar 22 09:29:59 2013 From: contact <@t> histocare.com (Contact HistoCare) Date: Fri Mar 22 09:30:05 2013 Subject: [Histonet] Leica Coverslipper Message-ID: <866A3404-4685-4FE2-A51F-BBA85603ABDD@histocare.com> Hi, The alerts are not from problems or malfunctions, they're from normal operation. For example the suction arm WILL alert if the cover glass is picked up and another is attached to the first one and somehow lands in a manner that makes the arm thinks the glass reservoir is empty. It WILL alert until someone quiets the alert, to let you know if the two racks are complete. It WILL alert once three racks have been coverslipped while it attempts to discharge a fourth rack and jam itself up. As someone mentioned previously, it definitely needs to be babysat just for normal operation. I agree that it is over-engineered , and yes the arm picking the slide up in the air is a poor design. The sakura uses an actuator to push the slide out and back in. In fact it's able to coverslip two slides in the same time the Leica does one. Although it is sufficient, It is not ideal for high volume operations. Personal preferences and contractual allegiances aside, the Sakura combo wins by a mile for bulletproof reliability and ability to handle large quantities without fail or alert. -------------- I have the Leica stainer and coverslipper, and I don't have anywhere near as many problems with the coverslipper as described by "Contact" below. Mine alerts once in a while; if his alerts that much, then something is seriously wrong. (The last time mine alerted that much, it needed a new "brain"-this is an older machine that had 5 circuit boards and one gave out-and one new sensor. Still worth it to us to fix it.) Anything as complex as staining and coverslipping robots will be fussy from time to time. But I love my Leica! Kathleen Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology & Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (848) 445-1443 FAX (732) 445-6905 From jqb7 <@t> cdc.gov Fri Mar 22 09:35:17 2013 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/OID/NCEZID)) Date: Fri Mar 22 09:35:41 2013 Subject: [Histonet] Leica Coverslipper In-Reply-To: <866A3404-4685-4FE2-A51F-BBA85603ABDD@histocare.com> References: <866A3404-4685-4FE2-A51F-BBA85603ABDD@histocare.com> Message-ID: I can honestly say we have used it for years and do not baby-sit it. Jeanine H. Bartlett Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 404-639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Contact HistoCare Sent: Friday, March 22, 2013 10:30 AM To: kgrobert@rci.rutgers.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica Coverslipper Hi, The alerts are not from problems or malfunctions, they're from normal operation. For example the suction arm WILL alert if the cover glass is picked up and another is attached to the first one and somehow lands in a manner that makes the arm thinks the glass reservoir is empty. It WILL alert until someone quiets the alert, to let you know if the two racks are complete. It WILL alert once three racks have been coverslipped while it attempts to discharge a fourth rack and jam itself up. As someone mentioned previously, it definitely needs to be babysat just for normal operation. I agree that it is over-engineered , and yes the arm picking the slide up in the air is a poor design. The sakura uses an actuator to push the slide out and back in. In fact it's able to coverslip two slides in the same time the Leica does one. Although it is sufficient, It is not ideal for high volume operations. Personal preferences and contractual allegiances aside, the Sakura combo wins by a mile for bulletproof reliability and ability to handle large quantities without fail or alert. -------------- I have the Leica stainer and coverslipper, and I don't have anywhere near as many problems with the coverslipper as described by "Contact" below. Mine alerts once in a while; if his alerts that much, then something is seriously wrong. (The last time mine alerted that much, it needed a new "brain"-this is an older machine that had 5 circuit boards and one gave out-and one new sensor. Still worth it to us to fix it.) Anything as complex as staining and coverslipping robots will be fussy from time to time. But I love my Leica! Kathleen Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology & Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (848) 445-1443 FAX (732) 445-6905_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Fri Mar 22 09:39:59 2013 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Fri Mar 22 09:40:04 2013 Subject: [Histonet] RE: Mouse bone blocks/sections, bone marrow smears In-Reply-To: <9B948E3AC1EA244FA291AEB778ECDCD02C906D@MSXMBXNSPRD02.acct.upmchs.net> References: <471953BC63077941B82C26A4338272B42F04E0@ORLEV03.hca.corpad.net> <7E0A77BFEB9A1E47A63F978E7116821F02AEC667@1upmc-msx11.acct.upmchs.net> <9B948E3AC1EA244FA291AEB778ECDCD02C906D@MSXMBXNSPRD02.acct.upmchs.net> Message-ID: <14E2C6176416974295479C64A11CB9AE016411AC2902@SBS2K8.premierlab.local> Jian I would fix for about 48 hours, then decal overnight in 10% formic acid, process to paraffin on a cycle of around 45 minutes per station, we sometimes process on hour long cycles with 3 absolutes and 3 xylenes, the sections cut just fine. We trim and then then soak the blocks for about 30 minutes on wet ice then section at 5 microns, let the slides drain a bit, shake off excess water and lay flat on a hot plate overnight set at 40C. If you are preparing bone marrow smears you want to keep then away from any 10% NBF so if you are collecting at necropsy stay as far away from the formalin as possible that will mess up your stain. Crack the femur and with a paint brush dipped in 5% EDTA collect the bone marrow from the femur and then "paint" it on the slide. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Laboratory Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 Work (303) 682-3949 Fax (303) 682-9060 Cell (303) 881-0763 liz@premierlab.com www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Yu, Jian Sent: Thursday, March 21, 2013 5:27 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Mouse bone blocks/sections, bone marrow smears Dear all, We like to make paraffin embedded bone of mouse tibia and cut longitudinal sections for H&E sections. Does anyone have a easy-to-follow protocol to prepare (fix and decal) the bone before processing? We routinely fix soft tissue (ie. mouse GI tract and liver) and send off to our pathology core for processing and embedding. Any tips on cutting the blocks is helpful too. These sections appear brittle. We are also interested in a practical protocol for making mouse BM smears and staining to determine cellularity changes after radiation. We just started working with the bone. Any advice will be very helpful and greatly appreciated. Thanks, Jian ******************************************************************* Jian Yu, Ph.D. University of Pittsburgh Cancer Institute Email: yuj2@upmc.edu ******************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BZIMMERM <@t> gru.edu Fri Mar 22 09:48:24 2013 From: BZIMMERM <@t> gru.edu (Zimmerman, Billie) Date: Fri Mar 22 09:51:19 2013 Subject: [Histonet] Billie's Blurbs-- High Tea at the Jekyll Club GSH Meeting April 12 - 14 Message-ID: <7B3DEB32E69C034EACB479059C5DE3FF75D5A3@EX-MLB-03.ad.georgiahealth.edu> For all of you history buffs, there's a treat for you on Jekyll Island. It's the Jekyll Island Club located minutes from where we will be meeting. The Jekyll Island Club hotel is a 4 star resort and is a National Historic Landmark. Even if you're not crazy about history, this place is worth the visit. When you walk in, you feel like you're walking into the book/movie "The Great Gatsby". There were folks on the lawn playing croquet! My brother told me that the hotel was haunted but he stayed there and didn't see a thing! ***I suggest High Tea at the Jekyll Island Club. It's from 4pm - 5:15pm and I think it's $14.95. ( It's much, much cheaper than the High Tea at the Empress Hotel on Victoria Island that we did last year.) It's served in the grand dining room. Please bring your camera because it's beautiful. Again, I'm asking you not to wear your speedo or crochet bikini. I'm attaching a link to the hotel, for you to see for yourself. I will say one last thing---THERE IS NO DOLLAR MENU. If you want to dine here bring some plastic, green stuff, or a friend who wants to buy your meal. http://www.jekyllclub.com/about-us/hotel-history/ Augusta State University and Georgia Health Sciences University have consolidated to become Georgia Regents University. Effective January 9, 2013, my email address has changed to BZIMMERM@gru.edu. Please update your address book to reflect this change. From HornHV <@t> archildrens.org Fri Mar 22 10:35:26 2013 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Fri Mar 22 10:35:41 2013 Subject: [Histonet] Leica Coverslipper In-Reply-To: References: <866A3404-4685-4FE2-A51F-BBA85603ABDD@histocare.com> Message-ID: <25A4DE08332B19499904459F00AAACB719BE1356B6@EVS1.archildrens.org> We too have one that is a work horse. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/OID/NCEZID) Sent: Friday, March 22, 2013 9:35 AM To: Contact HistoCare; kgrobert@rci.rutgers.edu; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Leica Coverslipper I can honestly say we have used it for years and do not baby-sit it. Jeanine H. Bartlett Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 404-639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Contact HistoCare Sent: Friday, March 22, 2013 10:30 AM To: kgrobert@rci.rutgers.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica Coverslipper Hi, The alerts are not from problems or malfunctions, they're from normal operation. For example the suction arm WILL alert if the cover glass is picked up and another is attached to the first one and somehow lands in a manner that makes the arm thinks the glass reservoir is empty. It WILL alert until someone quiets the alert, to let you know if the two racks are complete. It WILL alert once three racks have been coverslipped while it attempts to discharge a fourth rack and jam itself up. As someone mentioned previously, it definitely needs to be babysat just for normal operation. I agree that it is over-engineered , and yes the arm picking the slide up in the air is a poor design. The sakura uses an actuator to push the slide out and back in. In fact it's able to coverslip two slides in the same time the Leica does one. Although it is sufficient, It is not ideal for high volume operations. Personal preferences and contractual allegiances aside, the Sakura combo wins by a mile for bulletproof reliability and ability to handle large quantities without fail or alert. -------------- I have the Leica stainer and coverslipper, and I don't have anywhere near as many problems with the coverslipper as described by "Contact" below. Mine alerts once in a while; if his alerts that much, then something is seriously wrong. (The last time mine alerted that much, it needed a new "brain"-this is an older machine that had 5 circuit boards and one gave out-and one new sensor. Still worth it to us to fix it.) Anything as complex as staining and coverslipping robots will be fussy from time to time. But I love my Leica! Kathleen Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology & Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (848) 445-1443 FAX (732) 445-6905_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From Kim <@t> ncpath.com Fri Mar 22 11:02:52 2013 From: Kim <@t> ncpath.com (Kimmie Rabe) Date: Fri Mar 22 11:03:11 2013 Subject: [Histonet] RE: ventana counterstaining In-Reply-To: References: <77DD817201982748BC67D7960F2F76AF03E5B5@UWHC-MBX12.uwhis.hosp.wisc.edu> Message-ID: Hematoxylin and bluing on the ventana. But it is run through alcohols to xylene to coverslip. Possibly?? Although the histotechs say it comes off the ventana with the pink tint. Kim Rabe -----Original Message----- From: Lori Gemeinhardt [mailto:lorigemeinhardt@mac.com] Sent: Thursday, March 21, 2013 3:25 PM To: Sebree Linda A Cc: Kimmie Rabe; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: ventana counterstaining Hi Kimmie- Are you running your slides down in the routine H&E set up, ie are you using eosin 'contaminated' alcohols. Maybe you are lingering too long, and it is picking up some Eosin. Maybe?? Thanks- Lori On Mar 21, 2013, at 12:41 PM, Sebree Linda A wrote: > Kimmie, > > We use VMS' Hematoxylin II with no changes from how it has always stained. > > > > Linda A. Sebree > > University of Wisconsin Hospital & Clinics IHC/ISH Laboratory 600 > Highland Ave. > Madison, WI 53792 > > (608)265-6596 > FAX: (608)262-7174 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kimmie > Rabe > Sent: Thursday, March 21, 2013 11:28 AM > To: 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] ventana counterstaining > > Has anyone else had problems with the counterstain on the ventana being pink instead of that lovely pale blue color? Ventana says it's because we use Sta-On, but we don't always use Sta-On and the pink vs. blue staining doesn't correlate with whether Sta-On has been used or not. Don't get me wrong, I have nothing against pink. But if there is some problem with the hematoxylin we purchase from ventana or the dispensing or mixing thereof--we would like to know. > > Thanks! > > Kimmie E. Rabe, MD > North Central Pathology, PA > 3701 12th Street North, Suite 201 > St. Cloud, MN 56303 > Phone: 320-253-6554 > Fax: 320-253-1218 > kim@ncpath.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From chaul <@t> mail.med.upenn.edu Fri Mar 22 14:28:33 2013 From: chaul <@t> mail.med.upenn.edu (Lillian Chau) Date: Fri Mar 22 14:29:25 2013 Subject: [Histonet] Sirius Red Stain Troubleshooting Message-ID: <1973831796.9496262.1363980513546.JavaMail.root@zimbra.upenn.edu> Hi All- We have previously used Llewellyn sirius red protocol from Stainsfile with no issues to stain paneth cells. Recently, the sections are looking more like an H and E stain or completely red rather than the expected light blue (nuclei) and red (paneth) stain that we are accustomed to. The powder is from Sigma (its 2 years old) and we make a fresh solution for use each time. Hopefully you can provide some insight regarding this recent problem we are having. Thanks, Lillian From max_histo_00 <@t> yahoo.it Sat Mar 23 02:42:51 2013 From: max_histo_00 <@t> yahoo.it (Massimo) Date: Sat Mar 23 04:11:24 2013 Subject: [Histonet] Mallory triple stain In-Reply-To: <1363958665.76274.YahooMailNeo@web163104.mail.bf1.yahoo.com> References: <005001ce267f$27fcbce0$77f636a0$@ihctech.net> <1363958665.76274.YahooMailNeo@web163104.mail.bf1.yahoo.com> Message-ID: <1364024571.13517.YahooMailNeo@web172002.mail.ir2.yahoo.com> Hi all, you can find the original Mallory's trichrome stain with a typical example about the preparation of a transverse section of Amphioxus using the acid fuchsin-anilin? blue-orange G stain of Mallory (1901) at this address: http://www.mediafire.com/view/?ca96bvrdmp22te6 That's an abstract from the "A Text-book of Histology", please see at: http://www.mediafire.com/view/?5gjm10epdg303ja Kind Regards, Massimo Tosi "A humble Chemical Engineer who loves Histology" ________________________________ Da: Rene J Buesa A: "pruegg@ihctech.net" ; "histonet@lists.utsouthwestern.edu" Inviato: Venerd? 22 Marzo 2013 14:24 Oggetto: Re: [Histonet] Mallory triple stain Not really! There are scores of trichrome stains and Mallory is one of them with the characteristic that cell nuclei are not stained with hematoxylin, while in Masson TC they are. I personally prefer Mallory, it is more color "rich" and simpler to do. It was invented at the end of the XIX century and?Masson's in the early 1930's With TC stains the histologist's preferences rule, even when all stain the same but with different colors and intensities. Ren? J. From: "pruegg@ihctech.net" To: histonet@lists.utsouthwestern.edu Sent: Thursday, March 21, 2013 5:58 PM Subject: [Histonet] Mallory triple stain Does anyone know what this is about?? I think these people just want a trichrome stain and we are already doing a Masson's TC for them using aniline blue.? I think they are just asking for a modified version of TC and want to make sure we use aniline blue for the collagen stain???? Is Mallory Triple stain something other than a modified trichrome stain? "What we were asking for was Mallory Triple stain, which appears to be the synonymous with Mallory Aniline Blue, and Mallory Trichrome. Is that correct, that those three names are for the same staining technique? The main components of the stain that we were looking for were: aniline blue, orange G, and acid fuchsin. As far as I can tell all three techniques have those three components." Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E. Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 rueggihcconsultingpr@outlook.com This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From max_histo_00 <@t> yahoo.it Sat Mar 23 02:51:37 2013 From: max_histo_00 <@t> yahoo.it (Massimo) Date: Sat Mar 23 04:11:29 2013 Subject: [Histonet] Cheap digital camera microscope adapters? In-Reply-To: <1363960219.88345.YahooMailNeo@web163105.mail.bf1.yahoo.com> References: <514C3990.1040704@helsinki.fi> <1363960219.88345.YahooMailNeo@web163105.mail.bf1.yahoo.com> Message-ID: <1364025097.71166.YahooMailNeo@web172004.mail.ir2.yahoo.com> Hi, I have already answer?at a similar question. I hope it would be useful so I refer the whole text: ---------------------------------------------------- On the phototube of the "Lomo" tri-ocular head of my microscope I use a Nikon Coolpix P310 Camera without a "C" adaper. The phototube mounts a "Leitz" eyepiece 10x for photography (red dot) with a high exit eyepoint and a standard outside diameter barrel of 23.2 mm. The eyepiece is placed into an aluminum cylinder with an external diameter of 50.0 mm. and fixed by a plastic screw. The camera is hold on the cylinder by an "Universal Digiscoping Adapter" (diameter range: 43 - 65 mm.) brand "Vortex", which allows the movement of the camera in the X - Y directions. You can watch at the assembled system and some results on this folder link: http://www.mediafire.com/?qpo6i34ibh8o7 COST of the Nikon P310 camera in Italy on 29 May 2012: 350.00 Euro If you want to keep your "C" mount adaper you can choice among Nikon Coolpix cameras: 900, 995 and 4500. They are quite obsolete with cheap costs and you can still find something on "eBay". Despite its low cost they give good results. ---------------------------------------------------- Kind Regards, Massimo Tosi "A humble Chemical Engineer who loves Histology" ________________________________ Da: Rene J Buesa A: Mikael Niku ; "histonet@lists.utsouthwestern.edu" Inviato: Venerd? 22 Marzo 2013 14:50 Oggetto: Re: [Histonet] Cheap digital camera microscope adapters? If you have a Nikon Coolpix, you can adapt it to the microscope very easily although the Nikon Coolpix is not cheap. There is an article in "Histologic" [34(2):27-32 (2001)] that will give you good ideas. If you go to http://www.wpiinc.com/ there is an assortment of cheap "image capturing" devices of relatively good quality at low price. You can find image capturing devices at http://www.drinstruments.com/ for less than $100, but the images quality are wanting.? At http://www.srb-griturn.com/ they sell camera adapters that you can use to hold a camera to the ocular tube?to take photomicrographs of acceptable quality. Ren? J.? From: Mikael Niku To: histonet@lists.utsouthwestern.edu Sent: Friday, March 22, 2013 6:59 AM Subject: [Histonet] Cheap digital camera microscope adapters? Hello! I'm planning to purchase a set of digital cameras for our histology teaching laboratory, so that the students can do their histology work by taking microphotographs. Buying "real" microscope cameras would be too expensive as we need quite many (and our course microscopes don't have a camera tubus) so I'm thinking about fitting standard digital cameras in place of the eyepiece. The microscopes are good old Olympus CH2 ones and the cameras could be any affordable model. If anyone can recommend a functional but affordable adapter, fitting to a functional but affordable consumer level digital camera, please tell! With best regards, Mikael Niku, PhD University of Helsinki, Finland _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ibernard <@t> uab.edu Sun Mar 24 08:05:10 2013 From: ibernard <@t> uab.edu (Ian R Bernard) Date: Sun Mar 24 08:05:15 2013 Subject: [Histonet] Quality In AP Message-ID: I'm in the process of writing a comprehensive Quality Management Program for our AP department. I have references but would like some input from colleagues. - Sentinel event involves death or serious physical or psychological injury. - Near Miss fall short of that. Bottom-line, need some real life examples of near misses in Surgical pathology, Histopathology and Cytopathology. Send me you input IB From cls71877 <@t> gmail.com Sun Mar 24 09:58:15 2013 From: cls71877 <@t> gmail.com (Cristi Rigazio) Date: Sun Mar 24 09:58:24 2013 Subject: [Histonet] Quality In AP In-Reply-To: References: Message-ID: <37C9B2A7-F373-4881-8C54-5C405F30B7DF@gmail.com> During a tumor board conference, a pancreatic cancer case was being reviewed. The slide was shown and a pathologist pointed out the tissue was lung, not pancreas. The patient was scheduled for surgery the following day. It was promptly cancelled. This incident started in the lab when the wrong section was placed on the slide, how it got all the way to a final report and subsequent surgery scheduling, I can't answer. Is this the kind of example you are seeking? Kind regards, Cristi Sent from my iPhone On Mar 24, 2013, at 6:05 AM, Ian R Bernard wrote: > I'm in the process of writing a comprehensive Quality Management Program for our AP department. > > I have references but would like some input from colleagues. > > > - Sentinel event involves death or serious physical or psychological injury. > > - Near Miss fall short of that. > > Bottom-line, need some real life examples of near misses in Surgical pathology, Histopathology and Cytopathology. Send me you input > > IB > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Connie.Dieringer <@t> va.gov Thu Mar 7 14:18:37 2013 From: Connie.Dieringer <@t> va.gov (Dieringer, Connie J.) Date: Mon Mar 25 11:31:59 2013 Subject: [Histonet] Retention and Disposal of medical hardware Message-ID: I was wondering what everyone out there is doing in regards to how long you are keeping medical hardware such as pins/screws/metal plates/pacemakers, etc. that come from surgeries and how you are disposing of them? I know the CAP requirement for wet tissue is 2 weeks after the final report, but is hardware included in that? Do they go through any sort of decontamination before disposing of them? Connie Dieringer, HTL (ASCP) Histology Supervisor Michael E. DeBakey VA Medical Center Houston, TX Rm. 3A-215A, Bldg. 100 Tel.: 713-794-7259 Pager: 281-551-0171 Email: connie.dieringer@va.gov From Mark.Elliott <@t> hli.ubc.ca Mon Mar 25 09:28:34 2013 From: Mark.Elliott <@t> hli.ubc.ca (Mark Elliott) Date: Mon Mar 25 13:02:29 2013 Subject: [Histonet] EG1&2 antibodies In-Reply-To: <321175A4.509@mail.mrl.ubc.ca> References: <321175A4.509@mail.mrl.ubc.ca> Message-ID: <514FFCA2020000D60005CB6E@mail.hli.ubc.ca> I have been asked to do some staining for EG1&2 (ECP) in eosinophils but have not been able to find any suppliers for antibodies. All of my usual sources come up negative, including Biocompare. Anyone know of a supplier of the antibody? Thanks Mark ***CONFIDENTIALITY NOTICE*** This electronic message and any attachments are intended only for the use of the addressee and may contain information that is privileged and confidential. Any dissemination, distribution or copying of this communication by unauthorized individuals is strictly prohibited. If you have received this communication in error, please notify the sender immediately by reply e-mail and delete the original and all copies from your system. From lins0701 <@t> gmail.com Mon Mar 25 10:06:22 2013 From: lins0701 <@t> gmail.com (Sophia Lin) Date: Mon Mar 25 13:16:09 2013 Subject: [Histonet] hematoxylin back to deparaffin? Message-ID: We had a slight mistake with the deparaffin slides. A timer was not set, long story short. The deparaffinized slides went too far and proceeded to Hematoxylin. We have a linear stainer, so we took it back out from hematoxylin when we noticed the error and placed it into water to rinse. Then back to acid alchol. But the hematoxylin is still visible. Any tips? I have taken it back from completel H&E, just not hematoxylin... any advice would be great! Recutting isnt a good option. Thanks! From TJohnson <@t> gnf.org Mon Mar 25 12:07:49 2013 From: TJohnson <@t> gnf.org (Teri Johnson) Date: Mon Mar 25 14:01:41 2013 Subject: [Histonet] Re: hematoxylin back to deparaffin? Message-ID: <9F3CFEE76E51B64991C7485270890B4049734FAF@EX5.lj.gnf.org> Hi Sophia, If you plan to do immunostains that will eventually be hematoxylin counterstained, just let it be. If you need them to be completely decolorized, put them in 0.5% periodic acid and the rest of it should come out. I would be cautious however with what you do to the sections. It might make a difference to any subsequent testing downstream. Make sure you use two sets of controls, one with just regular depar, and one treated just like these slides. Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 From HornHV <@t> archildrens.org Mon Mar 25 13:03:24 2013 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Mon Mar 25 14:28:57 2013 Subject: [Histonet] RE: Retention and Disposal of medical hardware In-Reply-To: References: Message-ID: <25A4DE08332B19499904459F00AAACB719BE1356C4@EVS1.archildrens.org> We have a policy for hardware and if it includes any type of serial number, etc. it is sent back to surgery for the item to be documented as removed with the manufacturer. We also photograph them and include documentation in the gross dicatation. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dieringer, Connie J. Sent: Thursday, March 07, 2013 2:18 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Retention and Disposal of medical hardware I was wondering what everyone out there is doing in regards to how long you are keeping medical hardware such as pins/screws/metal plates/pacemakers, etc. that come from surgeries and how you are disposing of them? I know the CAP requirement for wet tissue is 2 weeks after the final report, but is hardware included in that? Do they go through any sort of decontamination before disposing of them? Connie Dieringer, HTL (ASCP) Histology Supervisor Michael E. DeBakey VA Medical Center Houston, TX Rm. 3A-215A, Bldg. 100 Tel.: 713-794-7259 Pager: 281-551-0171 Email: connie.dieringer@va.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From relia1 <@t> earthlink.net Mon Mar 25 14:09:11 2013 From: relia1 <@t> earthlink.net (Pam Barker) Date: Mon Mar 25 15:06:04 2013 Subject: [Histonet] RELIA Solutions HOT JOB ALERT!! Exclusive Opportunity with Brand New Dermpath lab in Southern CA on the beach!!! Message-ID: <205c01ce298c$3d60d700$b8228500$@earthlink.net> Hi Histonetters!! I hope your week is off to a great start. I have an exciting new opportunity to tell you about. This is a RELIA Solutions EXCLUSIVE!! I am working with a Dermatopathology practice in Southern CA that wants to hire an ASCP certified histotech with Mohs experience. This is a permanent full time day shift position. You would be the sole practitioner histo tech in their brand new state of the art lab. For more information please give me a call or shoot me an email. Thanks-Pam You can reach me toll free at 866-607-3542 until 5p EST or anytime on my cell phone at 407-353-5070 or via email at relia1@earthlink.net Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From gagnone <@t> KGH.KARI.NET Mon Mar 25 14:13:58 2013 From: gagnone <@t> KGH.KARI.NET (Gagnon, Eric) Date: Mon Mar 25 15:16:07 2013 Subject: [Histonet] Leica CV5030 Message-ID: <5F06C3AD0B27264CA20CFA986C87882E6FFCF5D0@EXCHANGEPV3.KGH.ON.CA> We have this same model which we use for routine staining, as well as the smaller Dako product we use for IHC and special stains. We've recently been advised to use microtome oil to lubricate the V-shaped grippers on the CV5030, as the previously-suggested grease was only causing more buildup (we also use Ventana labels). We had earlier received a software upgrade which lessened but certainly did not eliminate the gripping/dropping problems we had experienced that you described, Bruce. Show me a walkaway, problem-free coverslipper and I'll show you... I'm sure all models have their problems and challenges. Certainly the Dako CR100 has a simpler in-and-out, less-gravity-challenging mechanism than the Leica CV5030. Eric Gagnon Histology Laboratory Kingston General Hospital Kingston, Ontario, Canada Bruce wrote: >This coverslipper is over engineered. No self respecting histologist would design something that holds the slide a foot in the air by two "v" shaped grippers. Gravity sucks and the slide falls. I have a box of DISTROYED slides that I show the repairman. Can you imagine "Sorry Mr. Smith we can't read your biopsy because it splintered into pieces AFTER we did all the hard work." "Good luck with that health of yours." It is NOT compatible with Ventana labels, as the gum sticks to those grippers and the slide falls, or jams in the output rack. To tell you the truth, I loved the old Leica coverslipper. It had a "walking beam" and never broke a slide. I'm in the mood for a Sakaura. Bruce Gapinsk HT (ASCP) Chief Histologist Marin Medical Laboratories PathGroup SF From Stacy_McLaughlin <@t> cooley-dickinson.org Mon Mar 25 14:45:41 2013 From: Stacy_McLaughlin <@t> cooley-dickinson.org (Stacy McLaughlin) Date: Mon Mar 25 15:18:19 2013 Subject: [Histonet] RE: ventana counterstaining In-Reply-To: References: Message-ID: Yes! We've been experiencing the same issue intermittently for the past couple of weeks. The slides look like they did not get bluing reagent. I reported the problem to Ventana and they sent us a new bluing reagent dispenser. (Hoping this fixes it!) A couple of techs here thought the dispenser was difficult to press down on. A couple of weeks prior to this happening, we had a bluing reagent dispenser lose its prime and nothing was dispensed on the slides. Keeping my fingers crossed... Stacy McLaughlin, HT(ASCP) Histology Supervisor Cooley Dickinson Hospital 30 Locust Street Northampton, MA 01060 (413)582-2019 Stacy_McLaughlin@Cooley-Dickinson.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kimmie Rabe Sent: Thursday, March 21, 2013 12:28 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] ventana counterstaining Has anyone else had problems with the counterstain on the ventana being pink instead of that lovely pale blue color? Ventana says it's because we use Sta-On, but we don't always use Sta-On and the pink vs. blue staining doesn't correlate with whether Sta-On has been used or not. Don't get me wrong, I have nothing against pink. But if there is some problem with the hematoxylin we purchase from ventana or the dispensing or mixing thereof--we would like to know. Thanks! Kimmie E. Rabe, MD North Central Pathology, PA 3701 12th Street North, Suite 201 St. Cloud, MN 56303 Phone: 320-253-6554 Fax: 320-253-1218 kim@ncpath.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BZIMMERM <@t> gru.edu Mon Mar 25 16:14:29 2013 From: BZIMMERM <@t> gru.edu (Zimmerman, Billie) Date: Mon Mar 25 16:14:38 2013 Subject: [Histonet] GSH Jekyll Island April 12 - 14 Message-ID: <7B3DEB32E69C034EACB479059C5DE3FF75DEE7@EX-MLB-03.ad.georgiahealth.edu> Oh no! Tom Peters from Vanderbilt has told me I'm going to be in a pickle! That might be true if I decided to wear a crochet bikini to the Jekyll Island resort. Ha-ha Actually, Tom Peters will be speaking about the three Leadership Pickles. It's a session developed by the motivational speaker, Bob Farrell. There will be a "leadership pickles" survey conducted to find out what kind of pickle you might be. (everything you wanted to know about pickles but were afraid to ask....) I'm sure this will be an interesting subject for those of you in leadership roles and interested in finding out just what kind of pickle you might be. ***Here's just a bit of trivia for you. I found out that Bob Farrell is the person who started the Farrell's ice cream parlors. He sold out to Marriott in the early 1970's. Does anyone remember eating "the Zoo" at Farrell's? Look it up on YouTube. See you at the beach!! Augusta State University and Georgia Health Sciences University have consolidated to become Georgia Regents University. Effective January 9, 2013, my email address has changed to BZIMMERM@gru.edu. Please update your address book to reflect this change. From peddinti_2002us <@t> yahoo.co.in Mon Mar 25 17:52:00 2013 From: peddinti_2002us <@t> yahoo.co.in (kamal prasad) Date: Mon Mar 25 17:52:07 2013 Subject: [Histonet] Microscope/Camera Message-ID: <1364251920.13737.YahooMailNeo@web190902.mail.sg3.yahoo.com> Hello, ? We are interested in purchasing a brightfield microscope with ?4x, 10x, and 40x objectives (20x optional) as well as a 5MP minimum camera with associated image capture software. New or Used. Please contact espen@dbiosys.com. From ADuddey <@t> firsthealth.org Tue Mar 26 08:15:07 2013 From: ADuddey <@t> firsthealth.org (Duddey, Aimee) Date: Tue Mar 26 08:15:32 2013 Subject: [Histonet] Pathology requisitions Message-ID: Our facility, as many do now, uses electronic physician order entry. We struggle with the pathology and cytology portion of this. We still require the manual requisition to be able to obtain the necessary information like clinical history, specific specimen info, etc. Does anyone have a good electronic solution for the pathology/cytology requisition? Any ideas would be greatly appreciated. Aimee Duddey FirstHealth Moore Regional Hospital Pinehurst, NC 28394 aduddey@firsthealth.org 910-715-5286 From brett_connolly <@t> merck.com Tue Mar 26 08:17:45 2013 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Tue Mar 26 08:17:51 2013 Subject: [Histonet] What's your favorite mouse-on mouse detection kit Message-ID: Hi All, For those of you working in research on animals tissue I'm looking for a consensus of opinions on kits for HRP mouse- on -mouse detection. Would like to avoid biotinylation procedures (i.e.ARK). Thanks, Brett Brett M. Connolly, Ph.D. Principal Scientist, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From philip_manfre <@t> merck.com Tue Mar 26 08:27:00 2013 From: philip_manfre <@t> merck.com (Manfre, Philip) Date: Tue Mar 26 08:27:54 2013 Subject: [Histonet] RE: What's your favorite mouse-on mouse detection kit In-Reply-To: References: Message-ID: <558A4571351D0C42BD923F403F4198C4A6E91397CF@USCTMXP51014.merck.com> Hey Brett, We use Vector PK-2200. Usually works well. Phil. Philip Manfre, B.A., HT (ASCP) Associate Principal Scientist Merck Research Laboratories WP45-251 PO Box 4 West Point, PA 19486 215-652-9750 215-993-0383 (fax) philip_manfre@merck.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Connolly, Brett M Sent: Tuesday, March 26, 2013 9:18 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] What's your favorite mouse-on mouse detection kit Hi All, For those of you working in research on animals tissue I'm looking for a consensus of opinions on kits for HRP mouse- on -mouse detection. Would like to avoid biotinylation procedures (i.e.ARK). Thanks, Brett Brett M. Connolly, Ph.D. Principal Scientist, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From mhale <@t> MiracaLS.com Tue Mar 26 08:37:28 2013 From: mhale <@t> MiracaLS.com (Hale, Meredith) Date: Tue Mar 26 08:39:21 2013 Subject: [Histonet] HT Position Message-ID: <0E828EC51C7CC445A51E53F81B64E8C71F8BAB@s-irv-exchmb.PathologyPartners.intranet> Great opportunity for Histotechnician's in Crestview Hills, KY ! Tri-State Gastroenterology Associates is a multi-physician practice located in Northern Kentucky. Its mission is "To provide compassionate, high quality, cost-effective care to pateints with gastrointestinal problems" Looking for 2 Full Time HT/HTL's ( 32 hours a week is full time employement at this practice ) * Meet CLIA Grossing Requirements : CFR 493.1489, http://wwwn.cdc.gov/clia/regs/toc.aspx/ ,prior experience grossing GI specimens * Supervisor experience preferred * HT/HTL ASCP Certified * Experience with CLIA and CAP * Experience writing and maintaining policies and procedures * Prior laboratory start up experience is preferred * Ability to work independently Duties include: * Grossing * Embedding * Microtomy * Staining; routine and special stains only * Maintain supply orders and laboratory budget * Ability to be flexible and take on additional duties' as needed * Ability to work independently * Maintenance of laboratory for inspections * Maintenance of quality records Interested applicants should contact Meredith Hale; phone 214-596-2219 or through email mhale@miracals.com Meredith Hale HT (ASCP)cm Director External Sales Support Miraca Life Sciences 6655 North MacArthur Blvd. Irving , Texas 75039 Office: 214-596-2219 Cell: 469-648-8253 Fax: 1-866-688-3280 mhale@miracals.com> From thigginsht <@t> msn.com Tue Mar 26 09:18:10 2013 From: thigginsht <@t> msn.com (Tim Higgins) Date: Tue Mar 26 09:18:21 2013 Subject: [Histonet] Batch Controlling for IHC In-Reply-To: References: Message-ID: Hello All, Have you ever had a Pathologist say, if you have one tissue control (placed on the same slide as the patient tissue) per antibody, per patient case, but there are multiple blocks (same tissue type) from the same patient case, that would be batch controlling? Hope that made sense, just wondering if this is something others have dealt with. Thanks, Tim From jkrenz <@t> primecare.org Tue Mar 26 10:34:53 2013 From: jkrenz <@t> primecare.org (Renz, Jason) Date: Tue Mar 26 10:35:58 2013 Subject: [Histonet] Acid Fast Controls Message-ID: <1E0E2B14C709174B8AC2BE0AE7F76833A2F451E3CA@EXCHANGE2K7.staprimecare.org> We are in need of Acid Fast control blocks. Would anybody be interested in trading? We have very good Hpylori and Amyloid blocks. Thank you. Jason Renz HT(ASCP)/lab safety officer St. Alexius Medical Center Bismarck ND, 58506 (701)530-6733 ________________________________ This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. From mjdessoye <@t> commonwealthhealth.net Tue Mar 26 10:45:10 2013 From: mjdessoye <@t> commonwealthhealth.net (Dessoye, Michael J) Date: Tue Mar 26 10:45:29 2013 Subject: [Histonet] WT1 overstaining on Benchmark Ultra Message-ID: Hello Histonet, I have two strange occurrences that I'm trying to get to the bottom of. Running WT1 from Cell Marque on a Benchmark Ultra using iView detection and the recommended protocol. Two specimens (both of which happen to be pleural fluid) severely overstained, even leaving the slide with a brown background stain. This has only happened twice, and the slides were on different positions on different staining runs. They used the same antibody and detection kit, however other pleural fluid slides with WT1 are fine. Other slides that run on the same positions also stain ok. I am repeating the stains on the same tissues to try to repeat this but in the meantime has anyone experienced this condition or have any advice? The only time I've seen this condition before was due to a clogged vortex mixer, but it affected all the slides and is obviously not the case here. Thanks! Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdessoye@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Commonwealth Health. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. From lcolbert <@t> pathmdlabs.com Tue Mar 26 10:49:04 2013 From: lcolbert <@t> pathmdlabs.com (Laurie Colbert) Date: Tue Mar 26 10:49:26 2013 Subject: [Histonet] Control Tissue Message-ID: <12ECD7346266D74691EC2BFC75285E452F2D7AA4@BFL323E10.pathmdlabs.local> Does anyone have a good source for obtaining fresh tissue (formalin fixed) for IHC validation? I would need normal and tumor of various types. Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab From CThornton <@t> dahlchase.com Tue Mar 26 10:56:38 2013 From: CThornton <@t> dahlchase.com (Clare Thornton) Date: Tue Mar 26 10:59:31 2013 Subject: [Histonet] RE: WT1 overstaining on Benchmark Ultra In-Reply-To: References: Message-ID: I've seen the exact same thing happen...WT-1 antibody from Cell Marque, on Ventana instrumentation. Except we use Ultraview detection. I had 3 WT-1 slides running at the same time. Two were overstained like you describe, one was fine. The repeats were fine. The overstained slides almost look like they had been "cooked". Interesting! Clare J. Thornton, HTL(ASCP)QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dessoye, Michael J Sent: Tuesday, March 26, 2013 11:45 AM To: Histonet Subject: [Histonet] WT1 overstaining on Benchmark Ultra Hello Histonet, I have two strange occurrences that I'm trying to get to the bottom of. Running WT1 from Cell Marque on a Benchmark Ultra using iView detection and the recommended protocol. Two specimens (both of which happen to be pleural fluid) severely overstained, even leaving the slide with a brown background stain. This has only happened twice, and the slides were on different positions on different staining runs. They used the same antibody and detection kit, however other pleural fluid slides with WT1 are fine. Other slides that run on the same positions also stain ok. I am repeating the stains on the same tissues to try to repeat this but in the meantime has anyone experienced this condition or have any advice? The only time I've seen this condition before was due to a clogged vortex mixer, but it affected all the slides and is obviously not the case here. Thanks! Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdessoye@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Commonwealth Health. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akbitting <@t> geisinger.edu Tue Mar 26 11:07:10 2013 From: akbitting <@t> geisinger.edu (Bitting, Angela K.) Date: Tue Mar 26 11:09:51 2013 Subject: [Histonet] Batch Controlling for IHC In-Reply-To: References: Message-ID: <77F52EFAB8B1694B885E277C48FCD0F639B5D51C@GHSEXMBX2W8K1V.geisinger.edu> Technically, he would be right. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tim Higgins Sent: Tuesday, March 26, 2013 10:18 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Batch Controlling for IHC Hello All, Have you ever had a Pathologist say, if you have one tissue control (placed on the same slide as the patient tissue) per antibody, per patient case, but there are multiple blocks (same tissue type) from the same patient case, that would be batch controlling? Hope that made sense, just wondering if this is something others have dealt with. Thanks, Tim _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. ------------------------------------------------------------------------- This message was secured by ZixCorp(R). From SHEILA.HERRINGTON <@t> interiorhealth.ca Tue Mar 26 11:29:17 2013 From: SHEILA.HERRINGTON <@t> interiorhealth.ca (HERRINGTON, SHEILA) Date: Tue Mar 26 11:30:49 2013 Subject: [Histonet] RE: WT1 overstaining on Benchmark Ultra In-Reply-To: References: Message-ID: <9D5F3F245A5FE0458E46E9A71E3EE08C06B622D2CF@DC1SERV352.interiorhealth.ca> I have been experiencing the same random over staining issues with WT1 as well. I use the Benchmark Ultras with Ultraview. It started around the same time as a new lot number changed a few months back. I have tried everything, but being so random it is hard to find a solution. Sheila Herrington, IHC Kelowna General Grade 3, Immunohistochemistry, Histolopathogy Kelowna General Hospital -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Clare Thornton Sent: Tuesday, March 26, 2013 8:57 AM To: 'Dessoye, Michael J'; Histonet Subject: [Histonet] RE: WT1 overstaining on Benchmark Ultra I've seen the exact same thing happen...WT-1 antibody from Cell Marque, on Ventana instrumentation. Except we use Ultraview detection. I had 3 WT-1 slides running at the same time. Two were overstained like you describe, one was fine. The repeats were fine. The overstained slides almost look like they had been "cooked". Interesting! Clare J. Thornton, HTL(ASCP)QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dessoye, Michael J Sent: Tuesday, March 26, 2013 11:45 AM To: Histonet Subject: [Histonet] WT1 overstaining on Benchmark Ultra Hello Histonet, I have two strange occurrences that I'm trying to get to the bottom of. Running WT1 from Cell Marque on a Benchmark Ultra using iView detection and the recommended protocol. Two specimens (both of which happen to be pleural fluid) severely overstained, even leaving the slide with a brown background stain. This has only happened twice, and the slides were on different positions on different staining runs. They used the same antibody and detection kit, however other pleural fluid slides with WT1 are fine. Other slides that run on the same positions also stain ok. I am repeating the stains on the same tissues to try to repeat this but in the meantime has anyone experienced this condition or have any advice? The only time I've seen this condition before was due to a clogged vortex mixer, but it affected all the slides and is obviously not the case here. Thanks! Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdessoye@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Commonwealth Health. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tkngflght <@t> yahoo.com Tue Mar 26 12:40:43 2013 From: tkngflght <@t> yahoo.com (Cheryl) Date: Tue Mar 26 12:40:52 2013 Subject: [Histonet] eye pathology Message-ID: <1364319643.24961.YahooMailClassic@web161905.mail.bf1.yahoo.com> Hi Fellow 'Netters- ? Happy Passover, Easter, Solstice (...first day of Spring (last week, then it snowed!!) ? Is there a reference lab or facility that takes reference cases for eye pathology, specifically corneas?? Do they bill Medicare? ? Same question regarding brain (autopsy) pathology? ? Thanks in advance! Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab by helping one GREAT?Tech at a time.? 281.852.9457?Office 800.756.3309?Phone & Fax? admin@fullstaff.org Sign up for the FREE?newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please?include your name and specialty in the body of the email. From Meghan.Tucker <@t> ARS.USDA.GOV Tue Mar 26 14:20:47 2013 From: Meghan.Tucker <@t> ARS.USDA.GOV (Tucker, Meghan) Date: Tue Mar 26 14:21:38 2013 Subject: [Histonet] ASP 300S Stirrer Message-ID: <5A2DFCEFEE43D74C9685596FAABE71A20BD302@001FSN2MPN1-011.001f.mgd2.msft.net> Hello Histonet, We have an ASP 300S and have found that the stirrer at the bottom of the retort is not staying in place. It pops off of the base during the initial formalin fill and is not providing agitation throughout the process. Has anyone else with this processor had this issue? Any suggestions? Thank you in advance! Meghan Tucker USDA ARS This electronic message contains information generated by the USDA solely for the intended recipients. Any unauthorized interception of this message or the use or disclosure of the information it contains may violate the law and subject the violator to civil or criminal penalties. If you believe you have received this message in error, please notify the sender and delete the email immediately. From Joyce.Weems <@t> emoryhealthcare.org Tue Mar 26 14:27:08 2013 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Tue Mar 26 14:27:41 2013 Subject: [Histonet] RE: Pathology requisitions In-Reply-To: References: Message-ID: We have an electronic requisition that looks like the paper that we use in some units. The ORs didn't use it because they didn't have good access to computers from each room. But the GI labs and Breast Health used it successfully. A "Pathology" is ordered and fields pop up that the info is typed into. Two copies of the req prints - one in Histolgy with a bar code and one in the unit that is sent down with the specimen. That has served us well for several years. Unfortunately, with our partnerships with Emory and the upgrade to the system, we have to go back to paper. Maybe, someday.. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Duddey, Aimee Sent: Tuesday, March 26, 2013 9:15 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Pathology requisitions Our facility, as many do now, uses electronic physician order entry. We struggle with the pathology and cytology portion of this. We still require the manual requisition to be able to obtain the necessary information like clinical history, specific specimen info, etc. Does anyone have a good electronic solution for the pathology/cytology requisition? Any ideas would be greatly appreciated. Aimee Duddey FirstHealth Moore Regional Hospital Pinehurst, NC 28394 aduddey@firsthealth.org 910-715-5286 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From flnails <@t> texaschildrens.org Tue Mar 26 14:44:44 2013 From: flnails <@t> texaschildrens.org (Nails, Felton) Date: Tue Mar 26 14:44:50 2013 Subject: [Histonet] RE: Pathology requisitions In-Reply-To: References: Message-ID: What system are you using to place these orders in the OR? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Tuesday, March 26, 2013 2:27 PM To: 'Duddey, Aimee'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Pathology requisitions We have an electronic requisition that looks like the paper that we use in some units. The ORs didn't use it because they didn't have good access to computers from each room. But the GI labs and Breast Health used it successfully. A "Pathology" is ordered and fields pop up that the info is typed into. Two copies of the req prints - one in Histolgy with a bar code and one in the unit that is sent down with the specimen. That has served us well for several years. Unfortunately, with our partnerships with Emory and the upgrade to the system, we have to go back to paper. Maybe, someday.. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Duddey, Aimee Sent: Tuesday, March 26, 2013 9:15 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Pathology requisitions Our facility, as many do now, uses electronic physician order entry. We struggle with the pathology and cytology portion of this. We still require the manual requisition to be able to obtain the necessary information like clinical history, specific specimen info, etc. Does anyone have a good electronic solution for the pathology/cytology requisition? Any ideas would be greatly appreciated. Aimee Duddey FirstHealth Moore Regional Hospital Pinehurst, NC 28394 aduddey@firsthealth.org 910-715-5286 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ______________________________________________________________________ From hlukey <@t> msn.com Tue Mar 26 21:43:30 2013 From: hlukey <@t> msn.com (Hugh Luk) Date: Tue Mar 26 21:43:35 2013 Subject: [Histonet] RE: Histonet Digest, Vol 112, Issue 28 In-Reply-To: References: Message-ID: Hi Sophia, Your message is many hours old, and hopefully you figured out something already. Maybe this will help next time. What stains were you going to do with these slides? Our list of "Stain" removers may not be compatible with your desired stain. 1) 1% Acid (HCL), ~20 minutes, depending on hematoxylin blend. Acetic acid solutions will not remove the stain.2) 0.5% periodic acid, 5 minutes3) 5% Chromic acid, 5 minutes (wash water) followed by 1% sodium metabisulfite.4) Potassium permanganate, 5 minutes (wash water) followed by oxyalic acid.5) HIER6) Do not use any percentage of bleach. Bleach destroys proteins plus sections float right off. See a trend? Yeah, we've made our share of mistakes too. We also had several linear stainers. My coworker, Lynn, cut a few inches of polypropylene or nylon strapping (box shipping), bend 1.0 cm of one end 90 degrees, and insert between the container you don't want the slides to fall into + downstream. Put the piece over the top of the front third of the staining containers, to block the slide holders, not the slide bottoms. It saved us many times. Hope all turned out well,HughUH Cancer Center > ------------------------------ > > Date: Mon, 25 Mar 2013 08:06:22 -0700 > From: Sophia Lin > Subject: [Histonet] hematoxylin back to deparaffin? > To: histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset=ISO-8859-1 > > We had a slight mistake with the deparaffin slides. A timer was not set, > long story short. The deparaffinized slides went too far and proceeded to > Hematoxylin. We have a linear stainer, so we took it back out from > hematoxylin when we noticed the error and placed it into water to rinse. > Then back to acid alchol. But the hematoxylin is still visible. > > Any tips? I have taken it back from completel H&E, just not hematoxylin... > any advice would be great! Recutting isnt a good option. > > Thanks! > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 112, Issue 28 > ***************************************** From lins0701 <@t> gmail.com Wed Mar 27 08:15:13 2013 From: lins0701 <@t> gmail.com (Sophia Lin) Date: Wed Mar 27 08:15:28 2013 Subject: [Histonet] Wholes in tissue Message-ID: We noticed that once a section was placed on the water, the section has wholes that appear. They are not present when sectioning (not seen by the naked eye) but appear when the tissue is stretched. Is this an embedding issue? The "wholes" appear next to the tissue. This hasn't happened before just recently. Any thoughts? Sophia Sent from my iPhone From ibernard <@t> uab.edu Wed Mar 27 08:29:47 2013 From: ibernard <@t> uab.edu (Ian R Bernard) Date: Wed Mar 27 08:29:52 2013 Subject: [Histonet] Optimum room temperature for Histo & Cyto Lab Message-ID: Does anyone know about research or standards with this. Deg C or Deg F IB From BZIMMERM <@t> gru.edu Wed Mar 27 08:40:54 2013 From: BZIMMERM <@t> gru.edu (Zimmerman, Billie) Date: Wed Mar 27 08:41:01 2013 Subject: [Histonet] Students Please come to Jekyll Island!! Message-ID: <7B3DEB32E69C034EACB479059C5DE3FF75E65E@EX-MLB-03.ad.georgiahealth.edu> For the ALL INCLUSIVE price of $85, students my earn up to 15 CEU's through NSH. The Georgia Society for Histotechnology offers FREE membership for everyone. Robert Lott will be guiding you through the readiness for the HT/HTL exams. Find a roommate to split the cost of the room. If you can't find a classmate or friend, just find someone you can tolerate for about 48 to 72 hours. (Surely, you know someone within that tolerance range.) Way back, when the wheel was invented, I had a roommate that smoked in bed. It's rather paradoxical because she was in nursing school! So, find a roommate and enjoy all that Jekyll Island has to offer. No smoking allowed in rooms! Ha-ha See ya at the beach, Billie Augusta State University and Georgia Health Sciences University have consolidated to become Georgia Regents University. Effective January 9, 2013, my email address has changed to BZIMMERM@gru.edu. Please update your address book to reflect this change. From G.Spoelstra <@t> murdoch.edu.au Wed Mar 27 09:06:31 2013 From: G.Spoelstra <@t> murdoch.edu.au (Gerard Spoelstra) Date: Wed Mar 27 09:06:42 2013 Subject: [Histonet] Eosin not working for xylene free H&E Message-ID: Hi everyone, I have not had any success optimising the eosin counterstain in the absence of xylene. We routinely use 0.5% eosin Y in 95% ethanol. I've tried taking sections through to this to absolute ethanol but then air drying , but it leaves blotches of eosin. Using eosin 0.5% aqueous with a few drops of acetic acid doesn't appear to work, the keratin stains heavily but not elastin. After washing in water I leave for a short time in the oven then at room temperature for 5 minutes then coverslip with the dako atomatic cover slipper. For all the laboratories that have gone xylene free, are you just putting up with less than optimal eosin staining? Gerard Spoelstra Murdoch University From sdysart <@t> mirnarx.com Wed Mar 27 09:11:23 2013 From: sdysart <@t> mirnarx.com (Sarah Dysart) Date: Wed Mar 27 09:11:43 2013 Subject: [Histonet] RE: Eosin not working for xylene free H&E In-Reply-To: References: Message-ID: <8A70A9B2ECDD084DACFE6C59FCF86D5055F189CA@BL2PRD0711MB434.namprd07.prod.outlook.com> This exact reason is how I convinced my lab to go back to xylene!! I figure it's because there is water in your substitute. Because you can't see if there is water in it (from the air moisture or wherever...) it becomes frusterating and annoying to have to change out to fresh solutions sometimes several times a day!! Good Luck!! Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gerard Spoelstra Sent: Wednesday, March 27, 2013 9:07 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Eosin not working for xylene free H&E Hi everyone, I have not had any success optimising the eosin counterstain in the absence of xylene. We routinely use 0.5% eosin Y in 95% ethanol. I've tried taking sections through to this to absolute ethanol but then air drying , but it leaves blotches of eosin. Using eosin 0.5% aqueous with a few drops of acetic acid doesn't appear to work, the keratin stains heavily but not elastin. After washing in water I leave for a short time in the oven then at room temperature for 5 minutes then coverslip with the dako atomatic cover slipper. For all the laboratories that have gone xylene free, are you just putting up with less than optimal eosin staining? Gerard Spoelstra Murdoch University _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ibernard <@t> uab.edu Wed Mar 27 09:26:00 2013 From: ibernard <@t> uab.edu (Ian R Bernard) Date: Wed Mar 27 09:26:07 2013 Subject: [Histonet] Quality In AP In-Reply-To: <37C9B2A7-F373-4881-8C54-5C405F30B7DF@gmail.com> References: <37C9B2A7-F373-4881-8C54-5C405F30B7DF@gmail.com> Message-ID: Yes. Any more examples of near misses in histology and cytology? I will use these case studies and source of errors as examples. Although this may have been obvious human error with the wrong section on the slide, a systems approach to quality improvement could have prevented this incident. Building quality controls, assurances and improvement initiatives throughout the entire test cycle (pre analytical, analytical and post analytical) is key. We also need to be aware of latent systems errors that may or may not be in our control but must be considered as we try to improve quality and reduce errors for patient safety. IB -----Original Message----- From: Cristi Rigazio [mailto:cls71877@gmail.com] Sent: Sunday, March 24, 2013 9:58 AM To: Ian R Bernard Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Quality In AP During a tumor board conference, a pancreatic cancer case was being reviewed. The slide was shown and a pathologist pointed out the tissue was lung, not pancreas. The patient was scheduled for surgery the following day. It was promptly cancelled. This incident started in the lab when the wrong section was placed on the slide, how it got all the way to a final report and subsequent surgery scheduling, I can't answer. Is this the kind of example you are seeking? Kind regards, Cristi Sent from my iPhone On Mar 24, 2013, at 6:05 AM, Ian R Bernard wrote: > I'm in the process of writing a comprehensive Quality Management Program for our AP department. > > I have references but would like some input from colleagues. > > > - Sentinel event involves death or serious physical or psychological injury. > > - Near Miss fall short of that. > > Bottom-line, need some real life examples of near misses in Surgical pathology, Histopathology and Cytopathology. Send me you input > > IB > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Mhorne <@t> upei.ca Wed Mar 27 09:30:30 2013 From: Mhorne <@t> upei.ca (Margaret Horne) Date: Wed Mar 27 09:30:40 2013 Subject: [Histonet] Eosin not working for xylene free H&E In-Reply-To: References: Message-ID: <5152D856020000D100028FB5@oes-grpwise.novell.upei.ca> We don't have problems with our eosin after Xylene-free deparaffining. I use a 95% alcohol step just before the Eosin. Perhaps that might help. We don't air dry before coverslipping as my prof finds the quality not as good, so we use 100%IsOH before coverslipping; the mounting media you use will determine if it is ok to cover slip from IsOH. We just had a grad student compare Hot IsOH vs mineral oil vs Hot detergent as xylene free alternatives. I'll send you our final protocol if you like. Margaret Horne AVC, UPEI, "Gerard Spoelstra" 27/03/2013 11:06 AM >>> Hi everyone, I have not had any success optimising the eosin counterstain in the absence of xylene. We routinely use 0.5% eosin Y in 95% ethanol. I've tried taking sections through to this to absolute ethanol but then air drying , but it leaves blotches of eosin. Using eosin 0.5% aqueous with a few drops of acetic acid doesn't appear to work, the keratin stains heavily but not elastin. After washing in water I leave for a short time in the oven then at room temperature for 5 minutes then coverslip with the dako atomatic cover slipper. For all the laboratories that have gone xylene free, are you just putting up with less than optimal eosin staining? Gerard Spoelstra Murdoch University _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mhale <@t> MiracaLS.com Wed Mar 27 09:49:25 2013 From: mhale <@t> MiracaLS.com (Hale, Meredith) Date: Wed Mar 27 09:49:53 2013 Subject: [Histonet] HT Positions Message-ID: <0E828EC51C7CC445A51E53F81B64E8C71FB912@s-irv-exchmb.PathologyPartners.intranet> Great opportunity for Histotechnician's in Crestview Hills, KY ! Tri-State Gastroenterology Associates is a multi-physician practice located in Northern Kentucky. Its mission is "To provide compassionate, high quality, cost-effective care to pateints with gastrointestinal problems" Looking for 2 Full Time HT/HTL's ( 32 hours a week is full time employement at this practice ) * Meet CLIA Grossing Requirements : CFR 493.1489, http://wwwn.cdc.gov/clia/regs/toc.aspx/ ,prior experience grossing GI specimens * Supervisor experience preferred * HT/HTL ASCP Certified * Experience with CLIA and CAP * Experience writing and maintaining policies and procedures * Prior laboratory start up experience is preferred * Ability to work independently Duties include: * Grossing * Embedding * Microtomy * Staining; routine and special stains only * Maintain supply orders and laboratory budget * Ability to be flexible and take on additional duties' as needed * Ability to work independently * Maintenance of laboratory for inspections * Maintenance of quality records Interested applicants should contact Meredith Hale; phone 214-596-2219 or through email mhale@miracals.com Meredith Hale HT (ASCP)cm Director External Sales Support Miraca Life Sciences 6655 North MacArthur Blvd. Irving , Texas 75039 Office: 214-596-2219 Cell: 469-648-8253 Fax: 1-866-688-3280 mhale@miracals.com> From Timothy.Morken <@t> ucsfmedctr.org Wed Mar 27 10:17:18 2013 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Wed Mar 27 10:17:32 2013 Subject: [Histonet] Quality In AP In-Reply-To: References: <37C9B2A7-F373-4881-8C54-5C405F30B7DF@gmail.com> Message-ID: <761E2B5697F795489C8710BCC72141FF065D86@ex07.net.ucsf.edu> Oh, several types of problems I can think of in 30+ years of work: Labels switched on slides from two cases. Slides labeled correctly at microtome (hand written) but after staining the wrong paper label was put on the slide, and the label that should have been put on the slide was put on a different case. The correct slides and the wrong labels were both breast ca needle bx cases so the pathologist didn't catch that the mistake had happened. It only came to light when the other pathologist with the wrong label noticed the tissue section did not match those with IHC stains done on the same block. We traced back which slide must have gotten the wrong labels and notified the pathologist. He was just about to sign out the case. Cytology cell blocks mixed up. Several cytology cell blocks prepared together. Eventually the mistake was caught, four months later, during a random QA review. Outcome was chemo for a patient that didn't need it (long term problem is increased risk for cancer from the chemo), and no treatment for a patient that did need it (outcome, not treated as soon as could be). Tracing back we decided it was either mixed up at embedding or in cytology. Cytology was the probable source since blocks were (supposedly) always embedded individually at embedding. But we could not determine for sure where it happened. Two thyroid cases mixed up. At the microtome the cutter faced two thyroid blocks and put them back on ice. Slides were hand labeled for each block on the tray (against the rules - supposed to label slide only when block is being cut for sections). The cutter picked up a block, took sections and put them on the wrong slide. Same with second thyroid. The outcome was two patients getting the others treatment - one chemo, one surgery. I seems that no matter how many rules you put in place people always find a way to break them, intentionally (rushing, laziness is a type of intent) or not, it is all the same outcome - mistakes. We try to put in "engineered" controls - ways of doing things that cannot be short-cutted. That is hard to accomplish when any amount of "human nature" is involved. I tell my techs who fancy themselves as "fast" workers that NO ONE will remember how fast they were if they make major mistakes in the process. ONLY the mistakes will be remembered. Tim Morken -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ian R Bernard Sent: Wednesday, March 27, 2013 7:26 AM To: Cristi Rigazio Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Quality In AP Yes. Any more examples of near misses in histology and cytology? I will use these case studies and source of errors as examples. Although this may have been obvious human error with the wrong section on the slide, a systems approach to quality improvement could have prevented this incident. Building quality controls, assurances and improvement initiatives throughout the entire test cycle (pre analytical, analytical and post analytical) is key. We also need to be aware of latent systems errors that may or may not be in our control but must be considered as we try to improve quality and reduce errors for patient safety. IB -----Original Message----- From: Cristi Rigazio [mailto:cls71877@gmail.com] Sent: Sunday, March 24, 2013 9:58 AM To: Ian R Bernard Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Quality In AP During a tumor board conference, a pancreatic cancer case was being reviewed. The slide was shown and a pathologist pointed out the tissue was lung, not pancreas. The patient was scheduled for surgery the following day. It was promptly cancelled. This incident started in the lab when the wrong section was placed on the slide, how it got all the way to a final report and subsequent surgery scheduling, I can't answer. Is this the kind of example you are seeking? Kind regards, Cristi Sent from my iPhone On Mar 24, 2013, at 6:05 AM, Ian R Bernard wrote: > I'm in the process of writing a comprehensive Quality Management Program for our AP department. > > I have references but would like some input from colleagues. > > > - Sentinel event involves death or serious physical or psychological injury. > > - Near Miss fall short of that. > > Bottom-line, need some real life examples of near misses in Surgical pathology, Histopathology and Cytopathology. Send me you input > > IB > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TGoins <@t> mt.gov Wed Mar 27 11:11:48 2013 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Wed Mar 27 11:12:02 2013 Subject: [Histonet] RE: Eosin not working for xylene free H&E In-Reply-To: <8A70A9B2ECDD084DACFE6C59FCF86D5055F189CA@BL2PRD0711MB434.namprd07.prod.outlook.com> References: <8A70A9B2ECDD084DACFE6C59FCF86D5055F189CA@BL2PRD0711MB434.namprd07.prod.outlook.com> Message-ID: We use hot detergent solution for dewax and a xylene substitute (Pro-Par) after staining without problems. If there is water accumulating in the clearant, it can be removed with a molecular sieve placed in the staining trays. We don't have that problem here in Montana because it is dry dry dry. Tresa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sarah Dysart Sent: Wednesday, March 27, 2013 8:11 AM To: Gerard Spoelstra; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Eosin not working for xylene free H&E This exact reason is how I convinced my lab to go back to xylene!! I figure it's because there is water in your substitute. Because you can't see if there is water in it (from the air moisture or wherever...) it becomes frusterating and annoying to have to change out to fresh solutions sometimes several times a day!! Good Luck!! Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gerard Spoelstra Sent: Wednesday, March 27, 2013 9:07 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Eosin not working for xylene free H&E Hi everyone, I have not had any success optimising the eosin counterstain in the absence of xylene. We routinely use 0.5% eosin Y in 95% ethanol. I've tried taking sections through to this to absolute ethanol but then air drying , but it leaves blotches of eosin. Using eosin 0.5% aqueous with a few drops of acetic acid doesn't appear to work, the keratin stains heavily but not elastin. After washing in water I leave for a short time in the oven then at room temperature for 5 minutes then coverslip with the dako atomatic cover slipper. For all the laboratories that have gone xylene free, are you just putting up with less than optimal eosin staining? Gerard Spoelstra Murdoch University _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Mar 27 11:13:43 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Mar 27 11:13:46 2013 Subject: [Histonet] Eosin not working for xylene free H&E In-Reply-To: References: Message-ID: <1364400823.80656.YahooMailNeo@web163103.mail.bf1.yahoo.com> Totally xylene free staining is a consistent sequence described as: 1- dewax the sections with 2 washes of?liquid dish washer soap (any brand will do) in a 2% aq. sol. at 95?C of 1 minute each. 2- 2 consecutive 1 min. each wash with water at 90?C and to water at RT 3- stain as usual, any routine or special stains ? to pure ethanol 4- the stained sections are placed in an oven at 65?C for 5 minutes 5- cover as usual. No problems with the staining For more details please go to http://www.histosearch.com/rene.html?and read the "Xylene free" article. Ren? J. ? From: Gerard Spoelstra To: histonet@lists.utsouthwestern.edu Sent: Wednesday, March 27, 2013 10:06 AM Subject: [Histonet] Eosin not working for xylene free H&E Hi everyone, I have not had any success optimising the eosin counterstain in the absence of xylene. We routinely use 0.5% eosin Y in 95% ethanol. I've tried taking sections through to this to absolute ethanol but then air drying , but it leaves blotches of eosin.? Using eosin 0.5% aqueous with a few drops of acetic acid doesn't appear to work, the keratin stains heavily but not elastin. After washing in water I leave for a short time in the oven then at room temperature for 5 minutes then coverslip with the dako atomatic cover slipper.? For all the laboratories that have gone xylene free, are you just putting up with less than optimal eosin staining? Gerard Spoelstra? Murdoch University _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lins0701 <@t> gmail.com Wed Mar 27 11:26:45 2013 From: lins0701 <@t> gmail.com (Sophia Lin) Date: Wed Mar 27 11:26:49 2013 Subject: [Histonet] linear stainer hematoxylin staining issues Message-ID: We have the Hacker linear stainer, and for the past two weeks we had clean, crisp H&E staining. However, last Friday, something happened and our H&E stains no longer look crisp, but instead have a hazy, unreadable look. Nothing different has been done, and we have troubleshot for every possible scenario. After a clean set up, we noticed that the hematoxylin is not staining very well. We have it set for 2 minutes with agitation for a uniform staining. Hematoxylin is Harris Special (mercury free) from MCC and the eosin is with phloxine b from AMT. After the hematoxylin (2 buckets for total of 4.5 minutes), we use 1.5% acetic acid. It seems that the hematoxylin is not staining well. Any tips? We may need to consider increasing a bucket for hematoxylin? Thanks, Sophia From kkienitz <@t> orclinic.com Wed Mar 27 12:46:04 2013 From: kkienitz <@t> orclinic.com (Kienitz, Kari) Date: Wed Mar 27 12:48:19 2013 Subject: [Histonet] linear stainer hematoxylin staining issues In-Reply-To: References: Message-ID: <41400FFE517878449D89114DD2526090087EA15CB4@tocmail1.tocad.orclinic.com> If your acetic acid solution is made in-house....I would suggest remaking it, just in case it was accidently made stronger. Kari Kienitz HT, (ASCP) Histology Laboratory Portland Gastroenterology The Oregon Clinic 1111 NE 99th Ave Portland, OR 97220 503.935.8311 kkienitz@orclinic.com CONFIDENTIALITY WARNING: This e-mail and any attachments are for the exclusive and confidential use of the intended recipient. If you are not the intended recipient, please do not read, distribute or take action in reliance upon this missive. If you have received this in error, please notify the sender immediately by reply e-mail and delete this message and its attachments from your computer system. Thank you ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sophia Lin [lins0701@gmail.com] Sent: Wednesday, March 27, 2013 9:26 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] linear stainer hematoxylin staining issues We have the Hacker linear stainer, and for the past two weeks we had clean, crisp H&E staining. However, last Friday, something happened and our H&E stains no longer look crisp, but instead have a hazy, unreadable look. Nothing different has been done, and we have troubleshot for every possible scenario. After a clean set up, we noticed that the hematoxylin is not staining very well. We have it set for 2 minutes with agitation for a uniform staining. Hematoxylin is Harris Special (mercury free) from MCC and the eosin is with phloxine b from AMT. After the hematoxylin (2 buckets for total of 4.5 minutes), we use 1.5% acetic acid. It seems that the hematoxylin is not staining well. Any tips? We may need to consider increasing a bucket for hematoxylin? Thanks, Sophia _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ruhella.arjun <@t> gmail.com Wed Mar 27 12:56:04 2013 From: ruhella.arjun <@t> gmail.com (arjun ruhella) Date: Wed Mar 27 12:56:10 2013 Subject: [Histonet] linear stainer hematoxylin staining issues In-Reply-To: <41400FFE517878449D89114DD2526090087EA15CB4@tocmail1.tocad.orclinic.com> References: <41400FFE517878449D89114DD2526090087EA15CB4@tocmail1.tocad.orclinic.com> Message-ID: How do I unsubscribe from Histonet?? Thank you, Arjun On Wed, Mar 27, 2013 at 1:46 PM, Kienitz, Kari wrote: > If your acetic acid solution is made in-house....I would suggest remaking > it, just in case it was accidently made stronger. > > > Kari Kienitz HT, (ASCP) > Histology Laboratory > Portland Gastroenterology > The Oregon Clinic > 1111 NE 99th Ave > Portland, OR 97220 > 503.935.8311 > kkienitz@orclinic.com > > > > > CONFIDENTIALITY WARNING: This e-mail and any attachments are for the > exclusive and confidential use of the intended recipient. If you are not > the intended recipient, please do not read, distribute or take action in > reliance upon this missive. If you have received this in error, please > notify the sender immediately by reply e-mail and delete this message and > its attachments from your computer system. Thank you > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [ > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sophia Lin [ > lins0701@gmail.com] > Sent: Wednesday, March 27, 2013 9:26 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] linear stainer hematoxylin staining issues > > We have the Hacker linear stainer, and for the past two weeks we had clean, > crisp H&E staining. However, last Friday, something happened and our H&E > stains no longer look crisp, but instead have a hazy, unreadable look. > Nothing different has been done, and we have troubleshot for every possible > scenario. After a clean set up, we noticed that the hematoxylin is not > staining very well. We have it set for 2 minutes with agitation for a > uniform staining. Hematoxylin is Harris Special (mercury free) from MCC and > the eosin is with phloxine b from AMT. After the hematoxylin (2 buckets for > total of 4.5 minutes), we use 1.5% acetic acid. It seems that the > hematoxylin is not staining well. > > Any tips? We may need to consider increasing a bucket for hematoxylin? > > Thanks, > > Sophia > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From joelleweaver <@t> hotmail.com Wed Mar 27 14:23:17 2013 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Wed Mar 27 14:23:23 2013 Subject: [Histonet] Quality In AP In-Reply-To: <761E2B5697F795489C8710BCC72141FF065D86@ex07.net.ucsf.edu> References: , <37C9B2A7-F373-4881-8C54-5C405F30B7DF@gmail.com>, , <761E2B5697F795489C8710BCC72141FF065D86@ex07.net.ucsf.edu> Message-ID: "I tell my techs who fancy themselves as "fast" workers that NO ONE will remember how fast they were if they make major mistakes in the process. ONLY the mistakes will be remembered".Tim I had to re-post, so true and really can't be repeated enough. I had to learn this one the hard way Joelle Weaver MAOM, HTL (ASCP) QIHC > From: Timothy.Morken@ucsfmedctr.org > To: ibernard@uab.edu > Date: Wed, 27 Mar 2013 15:17:18 +0000 > Subject: RE: [Histonet] Quality In AP > CC: histonet@lists.utsouthwestern.edu > > Oh, several types of problems I can think of in 30+ years of work: > > Labels switched on slides from two cases. Slides labeled correctly at microtome (hand written) but after staining the wrong paper label was put on the slide, and the label that should have been put on the slide was put on a different case. The correct slides and the wrong labels were both breast ca needle bx cases so the pathologist didn't catch that the mistake had happened. It only came to light when the other pathologist with the wrong label noticed the tissue section did not match those with IHC stains done on the same block. We traced back which slide must have gotten the wrong labels and notified the pathologist. He was just about to sign out the case. > > Cytology cell blocks mixed up. Several cytology cell blocks prepared together. Eventually the mistake was caught, four months later, during a random QA review. Outcome was chemo for a patient that didn't need it (long term problem is increased risk for cancer from the chemo), and no treatment for a patient that did need it (outcome, not treated as soon as could be). Tracing back we decided it was either mixed up at embedding or in cytology. Cytology was the probable source since blocks were (supposedly) always embedded individually at embedding. But we could not determine for sure where it happened. > > Two thyroid cases mixed up. At the microtome the cutter faced two thyroid blocks and put them back on ice. Slides were hand labeled for each block on the tray (against the rules - supposed to label slide only when block is being cut for sections). The cutter picked up a block, took sections and put them on the wrong slide. Same with second thyroid. The outcome was two patients getting the others treatment - one chemo, one surgery. > > > I seems that no matter how many rules you put in place people always find a way to break them, intentionally (rushing, laziness is a type of intent) or not, it is all the same outcome - mistakes. > > We try to put in "engineered" controls - ways of doing things that cannot be short-cutted. That is hard to accomplish when any amount of "human nature" is involved. > > I tell my techs who fancy themselves as "fast" workers that NO ONE will remember how fast they were if they make major mistakes in the process. ONLY the mistakes will be remembered. > > Tim Morken > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ian R Bernard > Sent: Wednesday, March 27, 2013 7:26 AM > To: Cristi Rigazio > Cc: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Quality In AP > > Yes. Any more examples of near misses in histology and cytology? I will use these case studies and source of errors as examples. > > Although this may have been obvious human error with the wrong section on the slide, a systems approach to quality improvement could have prevented this incident. > > Building quality controls, assurances and improvement initiatives throughout the entire test cycle (pre analytical, analytical and post analytical) is key. > > We also need to be aware of latent systems errors that may or may not be in our control but must be considered as we try to improve quality and reduce errors for patient safety. > > IB > > -----Original Message----- > From: Cristi Rigazio [mailto:cls71877@gmail.com] > Sent: Sunday, March 24, 2013 9:58 AM > To: Ian R Bernard > Cc: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Quality In AP > > During a tumor board conference, a pancreatic cancer case was being reviewed. The slide was shown and a pathologist pointed out the tissue was lung, not pancreas. The patient was scheduled for surgery the following day. It was promptly cancelled. This incident started in the lab when the wrong section was placed on the slide, how it got all the way to a final report and subsequent surgery scheduling, I can't answer. Is this the kind of example you are seeking? > Kind regards, > Cristi > > Sent from my iPhone > > On Mar 24, 2013, at 6:05 AM, Ian R Bernard wrote: > > > I'm in the process of writing a comprehensive Quality Management Program for our AP department. > > > > I have references but would like some input from colleagues. > > > > > > - Sentinel event involves death or serious physical or psychological injury. > > > > - Near Miss fall short of that. > > > > Bottom-line, need some real life examples of near misses in Surgical pathology, Histopathology and Cytopathology. Send me you input > > > > IB > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWatson <@t> gnf.org Wed Mar 27 15:20:32 2013 From: JWatson <@t> gnf.org (James Watson) Date: Wed Mar 27 15:20:36 2013 Subject: [Histonet] Quality In AP In-Reply-To: <761E2B5697F795489C8710BCC72141FF065D86@ex07.net.ucsf.edu> References: <37C9B2A7-F373-4881-8C54-5C405F30B7DF@gmail.com> <761E2B5697F795489C8710BCC72141FF065D86@ex07.net.ucsf.edu> Message-ID: Years ago (30+) while working as a clinical lab tech in the Navy, the histotech was thrown out of the histology department for doing sloppy histology work as fast as he could, I volunteered to work in histology. After about 6 months of OJT and some training at AFIP I returned to the hospital and I assisted on an autopsy of a 30 y/o female that died of a malignant melanoma. The new pathologist mentioned to me that she had had a skin biopsy done a 5-6 years earlier that was reported out as being negative for melanoma. I dug the old slides and blocks out of the archives in the morgue and found the skin sample in the block was shriveled up in the paraffin. The original slides showed no melanin and morphology was terrible. I back processed the tissue, re fixed it, reprocessed it, and cut new sections. I restained the original slides and the new slides with H&E, Fontana Masson, and Warthin Starry for melanin; the new slides showed melanoma was present in the skin biopsy, the original slide quality was so bad (under fixed and poorly processed) that restaining barely showed melanin in the special stains. I showed the newly stained slides to the pathologist and he informed me that I cost the navy about a half a million dollars in a lawsuit. After that the pathologist insisted that all tissue was fully fixed and he did not care how long he had to wait for his slides, he wanted them all perfectly cut and stained perfectly. James Watson HT? ASCP GNF? Genomics Institute of the Novartis Research Foundation Tel??? 858-332-4647 Fax?? 858-812-1915 jwatson@gnf.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Wednesday, March 27, 2013 8:17 AM To: Ian R Bernard Cc: Histonet Subject: RE: [Histonet] Quality In AP Oh, several types of problems I can think of in 30+ years of work: Labels switched on slides from two cases. Slides labeled correctly at microtome (hand written) but after staining the wrong paper label was put on the slide, and the label that should have been put on the slide was put on a different case. The correct slides and the wrong labels were both breast ca needle bx cases so the pathologist didn't catch that the mistake had happened. It only came to light when the other pathologist with the wrong label noticed the tissue section did not match those with IHC stains done on the same block. We traced back which slide must have gotten the wrong labels and notified the pathologist. He was just about to sign out the case. Cytology cell blocks mixed up. Several cytology cell blocks prepared together. Eventually the mistake was caught, four months later, during a random QA review. Outcome was chemo for a patient that didn't need it (long term problem is increased risk for cancer from the chemo), and no treatment for a patient that did need it (outcome, not treated as soon as could be). Tracing back we decided it was either mixed up at embedding or in cytology. Cytology was the probable source since blocks were (supposedly) always embedded individually at embedding. But we could not determine for sure where it happened. Two thyroid cases mixed up. At the microtome the cutter faced two thyroid blocks and put them back on ice. Slides were hand labeled for each block on the tray (against the rules - supposed to label slide only when block is being cut for sections). The cutter picked up a block, took sections and put them on the wrong slide. Same with second thyroid. The outcome was two patients getting the others treatment - one chemo, one surgery. I seems that no matter how many rules you put in place people always find a way to break them, intentionally (rushing, laziness is a type of intent) or not, it is all the same outcome - mistakes. We try to put in "engineered" controls - ways of doing things that cannot be short-cutted. That is hard to accomplish when any amount of "human nature" is involved. I tell my techs who fancy themselves as "fast" workers that NO ONE will remember how fast they were if they make major mistakes in the process. ONLY the mistakes will be remembered. Tim Morken -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ian R Bernard Sent: Wednesday, March 27, 2013 7:26 AM To: Cristi Rigazio Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Quality In AP Yes. Any more examples of near misses in histology and cytology? I will use these case studies and source of errors as examples. Although this may have been obvious human error with the wrong section on the slide, a systems approach to quality improvement could have prevented this incident. Building quality controls, assurances and improvement initiatives throughout the entire test cycle (pre analytical, analytical and post analytical) is key. We also need to be aware of latent systems errors that may or may not be in our control but must be considered as we try to improve quality and reduce errors for patient safety. IB -----Original Message----- From: Cristi Rigazio [mailto:cls71877@gmail.com] Sent: Sunday, March 24, 2013 9:58 AM To: Ian R Bernard Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Quality In AP During a tumor board conference, a pancreatic cancer case was being reviewed. The slide was shown and a pathologist pointed out the tissue was lung, not pancreas. The patient was scheduled for surgery the following day. It was promptly cancelled. This incident started in the lab when the wrong section was placed on the slide, how it got all the way to a final report and subsequent surgery scheduling, I can't answer. Is this the kind of example you are seeking? Kind regards, Cristi Sent from my iPhone On Mar 24, 2013, at 6:05 AM, Ian R Bernard wrote: > I'm in the process of writing a comprehensive Quality Management Program for our AP department. > > I have references but would like some input from colleagues. > > > - Sentinel event involves death or serious physical or psychological injury. > > - Near Miss fall short of that. > > Bottom-line, need some real life examples of near misses in Surgical pathology, Histopathology and Cytopathology. Send me you input > > IB > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From delsuec <@t> gmail.com Wed Mar 27 16:30:45 2013 From: delsuec <@t> gmail.com (Deloris Carter) Date: Wed Mar 27 16:30:52 2013 Subject: [Histonet] Leica Cryostat Message-ID: We just upgraded our cryostat, and I'm wondering if anyone is in the market for a used Leica CM 1900. Let me know if you are interested. deloris.carter@shawneemission.org From ajohnston <@t> oregonmed.net Wed Mar 27 18:47:24 2013 From: ajohnston <@t> oregonmed.net (Johnston, Amy) Date: Wed Mar 27 18:47:30 2013 Subject: [Histonet] Methanol Message-ID: <8F6C0286892B2544B10E5F31F1C7075D27E1363E@IPSPRDMBOX1.oregonmed.net> Hello all, I work in a medical lab that has recently expanded to histology and we are working on our chemical hygiene plan. We have already done the initial air sample testing for formaldehyde and xylene, but we were told by someone at OSHA that we need to test for methanol as well. The only place that methanol is used is on one of the med tech's machines and it is only 4 ml occasionally. I'm not sure if this is something we need to include in our plan, it was never tested for in the past. How can we find out specifically which chemicals we need to monitor? Amy From hlukey <@t> msn.com Wed Mar 27 19:49:05 2013 From: hlukey <@t> msn.com (Hugh Luk) Date: Wed Mar 27 19:49:12 2013 Subject: [Histonet] Histonet In-Reply-To: References: Message-ID: Sophia, Holes like you mentioned, are probably from the polymers or impurities in your embedding wax separating. Gayle Callus mentioned this several times (see histosearch), and recommended "Stirring" the embedding paraffin every so often (before every use). It could also be your tissue processing. Check to make sure it is properly processed. Embed without letting the wax solidify before doing so. You may have a bad batch of reagents? As for the stainer, Hazy nuclei, as long as you are sure your hemotoxylin is fine, are indicative of incomplete deparaffinzation, which could mean your OVEN or your reagents. It could also be your acid/base solutions. Note: Have you started a new lot # of wax? How about xylene (xylene-substiture) or alcohols? I have heard people saying X-brand paraffin was having problems with impurities that could cause these problems. I also heard it was solved last year. Also, your stainer has very little leeway in deparaffinzation. You might have to do things by hand until this problem is resolved. You know, deparaffinize to water using the longer method, then finish the H&E in the stainer. Does this help? Good luck (whoa, you have your work cut out for you), Hugh UH Cancer Center Hawaii > Message: 6 > Date: Wed, 27 Mar 2013 06:15:13 -0700 > From: Sophia Lin > Subject: [Histonet] Wholes in tissue > To: Histonet > Message-ID: > Content-Type: text/plain; charset=us-ascii > > We noticed that once a section was placed on the water, the section has wholes that appear. They are not present when sectioning (not seen by the naked eye) but appear when the tissue is stretched. Is this an embedding issue? The "wholes" appear next to the tissue. This hasn't happened before just recently. > > Any thoughts? > > Sophia > > > ------------------------------ > > Message: 17 > Date: Wed, 27 Mar 2013 09:26:45 -0700 > From: Sophia Lin > Subject: [Histonet] linear stainer hematoxylin staining issues > To: histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset=ISO-8859-1 > > We have the Hacker linear stainer, and for the past two weeks we had clean, > crisp H&E staining. However, last Friday, something happened and our H&E > stains no longer look crisp, but instead have a hazy, unreadable look. > Nothing different has been done, and we have troubleshot for every possible > scenario. After a clean set up, we noticed that the hematoxylin is not > staining very well. We have it set for 2 minutes with agitation for a > uniform staining. Hematoxylin is Harris Special (mercury free) from MCC and > the eosin is with phloxine b from AMT. After the hematoxylin (2 buckets for > total of 4.5 minutes), we use 1.5% acetic acid. It seems that the > hematoxylin is not staining well. > > Any tips? We may need to consider increasing a bucket for hematoxylin? > > Thanks, > > Sophia > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 112, Issue 30 > ***************************************** From BSullivan <@t> virtua.org Thu Mar 28 06:58:10 2013 From: BSullivan <@t> virtua.org (Sullivan, Beatrice) Date: Thu Mar 28 06:58:18 2013 Subject: [Histonet] Quality In AP In-Reply-To: References: <37C9B2A7-F373-4881-8C54-5C405F30B7DF@gmail.com> <761E2B5697F795489C8710BCC72141FF065D86@ex07.net.ucsf.edu> Message-ID: <6932520047F7EE46B512E9801344F160164F13E8@EXCHANGEMB-2.Virtua.org> Profound what impact bad histology can have on someone. Makes me very sad. Beatrice Sullivan Corporate Histology Manager Virtua,Voorhees -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of James Watson Sent: Wednesday, March 27, 2013 4:21 PM To: Ian R Bernard Cc: Histonet Subject: RE: [Histonet] Quality In AP Years ago (30+) while working as a clinical lab tech in the Navy, the histotech was thrown out of the histology department for doing sloppy histology work as fast as he could, I volunteered to work in histology. After about 6 months of OJT and some training at AFIP I returned to the hospital and I assisted on an autopsy of a 30 y/o female that died of a malignant melanoma. The new pathologist mentioned to me that she had had a skin biopsy done a 5-6 years earlier that was reported out as being negative for melanoma. I dug the old slides and blocks out of the archives in the morgue and found the skin sample in the block was shriveled up in the paraffin. The original slides showed no melanin and morphology was terrible. I back processed the tissue, re fixed it, reprocessed it, and cut new sections. I restained the original slides and the new slides with H&E, Fontana Masson, and Warthin Starry for melanin; the new slides showed melanoma was present in the skin biopsy, the original slide quality was so bad (under fixed and poorly processed) that restaining barely showed melanin in the special stains. I showed the newly stained slides to the pathologist and he informed me that I cost the navy about a half a million dollars in a lawsuit. After that the pathologist insisted that all tissue was fully fixed and he did not care how long he had to wait for his slides, he wanted them all perfectly cut and stained perfectly. James Watson HT? ASCP GNF? Genomics Institute of the Novartis Research Foundation Tel??? 858-332-4647 Fax?? 858-812-1915 jwatson@gnf.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Wednesday, March 27, 2013 8:17 AM To: Ian R Bernard Cc: Histonet Subject: RE: [Histonet] Quality In AP Oh, several types of problems I can think of in 30+ years of work: Labels switched on slides from two cases. Slides labeled correctly at microtome (hand written) but after staining the wrong paper label was put on the slide, and the label that should have been put on the slide was put on a different case. The correct slides and the wrong labels were both breast ca needle bx cases so the pathologist didn't catch that the mistake had happened. It only came to light when the other pathologist with the wrong label noticed the tissue section did not match those with IHC stains done on the same block. We traced back which slide must have gotten the wrong labels and notified the pathologist. He was just about to sign out the case. Cytology cell blocks mixed up. Several cytology cell blocks prepared together. Eventually the mistake was caught, four months later, during a random QA review. Outcome was chemo for a patient that didn't need it (long term problem is increased risk for cancer from the chemo), and no treatment for a patient that did need it (outcome, not treated as soon as could be). Tracing back we decided it was either mixed up at embedding or in cytology. Cytology was the probable source since blocks were (supposedly) always embedded individually at embedding. But we could not determine for sure where it happened. Two thyroid cases mixed up. At the microtome the cutter faced two thyroid blocks and put them back on ice. Slides were hand labeled for each block on the tray (against the rules - supposed to label slide only when block is being cut for sections). The cutter picked up a block, took sections and put them on the wrong slide. Same with second thyroid. The outcome was two patients getting the others treatment - one chemo, one surgery. I seems that no matter how many rules you put in place people always find a way to break them, intentionally (rushing, laziness is a type of intent) or not, it is all the same outcome - mistakes. We try to put in "engineered" controls - ways of doing things that cannot be short-cutted. That is hard to accomplish when any amount of "human nature" is involved. I tell my techs who fancy themselves as "fast" workers that NO ONE will remember how fast they were if they make major mistakes in the process. ONLY the mistakes will be remembered. Tim Morken -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ian R Bernard Sent: Wednesday, March 27, 2013 7:26 AM To: Cristi Rigazio Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Quality In AP Yes. Any more examples of near misses in histology and cytology? I will use these case studies and source of errors as examples. Although this may have been obvious human error with the wrong section on the slide, a systems approach to quality improvement could have prevented this incident. Building quality controls, assurances and improvement initiatives throughout the entire test cycle (pre analytical, analytical and post analytical) is key. We also need to be aware of latent systems errors that may or may not be in our control but must be considered as we try to improve quality and reduce errors for patient safety. IB -----Original Message----- From: Cristi Rigazio [mailto:cls71877@gmail.com] Sent: Sunday, March 24, 2013 9:58 AM To: Ian R Bernard Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Quality In AP During a tumor board conference, a pancreatic cancer case was being reviewed. The slide was shown and a pathologist pointed out the tissue was lung, not pancreas. The patient was scheduled for surgery the following day. It was promptly cancelled. This incident started in the lab when the wrong section was placed on the slide, how it got all the way to a final report and subsequent surgery scheduling, I can't answer. Is this the kind of example you are seeking? Kind regards, Cristi Sent from my iPhone On Mar 24, 2013, at 6:05 AM, Ian R Bernard wrote: > I'm in the process of writing a comprehensive Quality Management Program for our AP department. > > I have references but would like some input from colleagues. > > > - Sentinel event involves death or serious physical or psychological injury. > > - Near Miss fall short of that. > > Bottom-line, need some real life examples of near misses in Surgical > pathology, Histopathology and Cytopathology. Send me you input > > IB > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message, and any included attachments, are from Virtua Health or its related affiliates and is intended only for the addressee(s). The information contained herein is privileged, proprietary or may include confidential information and/or protected patient health information. Any unauthorized review, forwarding, printing, copying, distributing, or otherwise disseminating or taking any action based on such information is strictly prohibited. If you have received this message in error, or have reason to believe you are not authorized to receive it, please delete this message promptly and notify the sender by e-mail with a copy to ISSECURITY@virtua.org. Thank you From PMonfils <@t> Lifespan.org Thu Mar 28 09:38:39 2013 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Thu Mar 28 09:38:46 2013 Subject: [Histonet] Methanol Message-ID: I would contact OSHA and tell them the specifics. The possible vapor contribution of 4 ml of methanol, if it is even measurable, is certainly insignificant from a health/safety perspective. From Dingersoll <@t> aplaboratories.com Thu Mar 28 10:53:44 2013 From: Dingersoll <@t> aplaboratories.com (Dingersoll@aplaboratories.com) Date: Thu Mar 28 10:53:55 2013 Subject: [Histonet] Expired slides Message-ID: <20130328085344.073ecbdb5144cf8a05e574ee22bfb11a.ca5daecafe.wbe@email17.secureserver.net> dingersoll@aplabo From gayle.callis <@t> bresnan.net Thu Mar 28 11:00:08 2013 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Thu Mar 28 11:00:21 2013 Subject: [Histonet] Re: Holes in paraffin, remedies Message-ID: <000101ce2bcd$53b25810$fb170830$@bresnan.net> Hugh wrote: Sophia, Holes like you mentioned, are probably from the polymers or impurities in your embedding wax separating. Gayle Callus mentioned this several times (see histosearch), and recommended "Stirring" the embedding paraffin every so often (before every use). It could also be your tissue processing. Check to make sure it is properly processed. Embed without letting the wax solidify before doing so. You may have a bad batch of reagents? As for the stainer, Hazy nuclei, as long as you are sure your hemotoxylin is fine, are indicative of incomplete deparaffinzation, which could mean your OVEN or your reagents. It could also be your acid/base solutions. Note: Have you started a new lot # of wax? How about xylene (xylene-substiture) or alcohols? I have heard people saying X-brand paraffin was having problems with impurities that could cause these problems. I also heard it was solved last year. Also, your stainer has very little leeway in deparaffinzation. You might have to do things by hand until this problem is resolved. You know, deparaffinize to water using the longer method, then finish the H&E in the stainer. Does this help? Good luck (whoa, you have your work cut out for you), **************************************************************************** *********************** Hugh, Thank you for the kind words and your reply was right on the money. In fact, we stirred the paraffin daily before embedding, since the settling of polymers is not just occasional. Also, clean your embedding center frequently, before adding more paraffin. There is a clever little test for paraffin carryover into your rehydration alcohols when removing paraffin. 1) Use a glass beaker, add a few ml of used alcohol starting with last 95% just before 70% in deparaffinization setup. 2) Pipette a few mls of tap water into this aliquot of 95% and look for cloudiness. If a white cloud occurs, you have paraffin carryover all the way down your deparaffinization process. 3) If you don't see cloudy in this last 95%, test the 95% before this one, and keep going backwards towards the xylene or xylene substitute. If the next 95% is cloudy, there is paraffin carryover. Change out that alcohol station and all the ones before that including the xylene/xylene substitute. Also, you can do "rotation" where you move the second ( closest to water) 95% into first 95% spot, then replace the second 95% with fresh, replace all 100% and xylene/xyl sub as these latter are probably heavily contaminated with paraffin which is carrying over into your alcohols at some level. We used two or three xylene/xylene substitute changes (three preferred when doing IHC at 5 min/change) , two (or three) 100%, two 95% and one 70% alcohol changes before distilled water, 3 minutes per change, with hand staining. Change distilled water frequently, fresh daily and more changes when staining many slides as you don't need alcohol carry over into your hematoxylin. Careful monitoring of your solvents and distilled water should allow better hematoxylin staining. We also changed our acidic solution after hematoxylin and bluing solutions daily, these solutions are cheap plus always doing a 1 minute running water rinse after hematoxylin, acidic solution, and bluing. If you don't have running water rinsing, at least change your water rinses before each staining run. This all sounds very picky, but our H&E staining was very successful. Gayle Callis HTL/HT/MT(ASCP) From ibernard <@t> uab.edu Thu Mar 28 11:45:57 2013 From: ibernard <@t> uab.edu (Ian R Bernard) Date: Thu Mar 28 11:46:02 2013 Subject: [Histonet] Validation Method for Auto H&E stainer Message-ID: I'm looking for a benchmark or researched protocol. IB From Steven.Swartwood <@t> cshs.org Thu Mar 28 12:40:22 2013 From: Steven.Swartwood <@t> cshs.org (Swartwood, Steven J) Date: Thu Mar 28 12:40:28 2013 Subject: [Histonet] Biocare Decloaker Nx Gen Message-ID: <959202AC61AEF942968646EC66E2BE3E407C11@CSHSMSGMBX05.CSMC.EDU> Has anyone ever used the Biocare Decloaking Chamber Nx Gen for epitope unmasking (heat retrieval)? Also does anyone have any personal pros/cons with this instrument? We are thinking about getting one because of inconsistencies with our current method using a vegetable steamer. We use many different tissue types both animal and human. Also we use many different antibodies. Currently with the vegetable steamer we've used Citrate, EDTA, and Tris-EDTA combinations. We've used times from 30-60 minutes, and even tried 30 minutes x 2 or 40 minutes x 2, but we still see some inconsistent staining. To make sure it wasn't due to fixation vimentin staining was performed and shows even staining. It's just certain antibodies that are giving us trouble even from multiple different companies. Any advice would be greatly appreciated and very beneficial to our work pertaining to the instrument. Thanks again everyone have a great end of the week, Steven Swartwood HT(ASCP) steven.swartwood@cshs.org IMPORTANT WARNING: This message is intended for the use of the person or entity to which it is addressed and may contain information that is privileged and confidential, the disclosure of which is governed by applicable law. If the reader of this message is not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this information is STRICTLY PROHIBITED. If you have received this message in error, please notify us immediately by calling (310) 423-6428 and destroy the related message. Thank You for your cooperation. From ibernard <@t> uab.edu Thu Mar 28 12:43:53 2013 From: ibernard <@t> uab.edu (Ian R Bernard) Date: Thu Mar 28 12:43:58 2013 Subject: [Histonet] RE: Biocare Decloaker Nx Gen In-Reply-To: <959202AC61AEF942968646EC66E2BE3E407C11@CSHSMSGMBX05.CSMC.EDU> References: <959202AC61AEF942968646EC66E2BE3E407C11@CSHSMSGMBX05.CSMC.EDU> Message-ID: I would welcome input hear as well since we are looking at buying one as well. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Swartwood, Steven J Sent: Thursday, March 28, 2013 12:40 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Biocare Decloaker Nx Gen Has anyone ever used the Biocare Decloaking Chamber Nx Gen for epitope unmasking (heat retrieval)? Also does anyone have any personal pros/cons with this instrument? We are thinking about getting one because of inconsistencies with our current method using a vegetable steamer. We use many different tissue types both animal and human. Also we use many different antibodies. Currently with the vegetable steamer we've used Citrate, EDTA, and Tris-EDTA combinations. We've used times from 30-60 minutes, and even tried 30 minutes x 2 or 40 minutes x 2, but we still see some inconsistent staining. To make sure it wasn't due to fixation vimentin staining was performed and shows even staining. It's just certain antibodies that are giving us trouble even from multiple different companies. Any advice would be greatly appreciated and very beneficial to our work pertaining to the instrument. Thanks again everyone have a great end of the week, Steven Swartwood HT(ASCP) steven.swartwood@cshs.org IMPORTANT WARNING: This message is intended for the use of the person or entity to which it is addressed and may contain information that is privileged and confidential, the disclosure of which is governed by applicable law. If the reader of this message is not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this information is STRICTLY PROHIBITED. If you have received this message in error, please notify us immediately by calling (310) 423-6428 and destroy the related message. Thank You for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Thu Mar 28 13:00:34 2013 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Thu Mar 28 13:00:49 2013 Subject: [Histonet] RE: Biocare Decloaker Nx Gen In-Reply-To: References: <959202AC61AEF942968646EC66E2BE3E407C11@CSHSMSGMBX05.CSMC.EDU> Message-ID: <761E2B5697F795489C8710BCC72141FF066066@ex07.net.ucsf.edu> We tried the Decloaking Chamber Plus a couple years ago after using the Decloaking chamber for years. The Decloaking chamber gave us great results, but the record keeping (time, temp, pressure) for four Decloakers was time consuming and irritating. The PLUS model uses a USB stick to record all that so we didn't have to. A great improvement. But we ended up going with Bond stainers which obviated all that. However, it was a good upgrade, and the NX looks even better - Pre-programmed, easier to use, and with the USB feature. Pressure cookers and waterbaths generally give more consistent results than steamers since there is variation in the heating within a steam chamber so I think it will help you a lot to change over. With the new instruments' automation it should be painless to operate. Tim Morken UCSF Medical Center Pathology Dept -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ian R Bernard Sent: Thursday, March 28, 2013 10:44 AM To: Swartwood, Steven J; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Biocare Decloaker Nx Gen I would welcome input hear as well since we are looking at buying one as well. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Swartwood, Steven J Sent: Thursday, March 28, 2013 12:40 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Biocare Decloaker Nx Gen Has anyone ever used the Biocare Decloaking Chamber Nx Gen for epitope unmasking (heat retrieval)? Also does anyone have any personal pros/cons with this instrument? We are thinking about getting one because of inconsistencies with our current method using a vegetable steamer. We use many different tissue types both animal and human. Also we use many different antibodies. Currently with the vegetable steamer we've used Citrate, EDTA, and Tris-EDTA combinations. We've used times from 30-60 minutes, and even tried 30 minutes x 2 or 40 minutes x 2, but we still see some inconsistent staining. To make sure it wasn't due to fixation vimentin staining was performed and shows even staining. It's just certain antibodies that are giving us trouble even from multiple different companies. Any advice would be greatly appreciated and very beneficial to our work pertaining to the instrument. Thanks again everyone have a great end of the week, Steven Swartwood HT(ASCP) steven.swartwood@cshs.org IMPORTANT WARNING: This message is intended for the use of the person or entity to which it is addressed and may contain information that is privileged and confidential, the disclosure of which is governed by applicable law. If the reader of this message is not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this information is STRICTLY PROHIBITED. If you have received this message in error, please notify us immediately by calling (310) 423-6428 and destroy the related message. Thank You for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Wanda.Smith <@t> HCAhealthcare.com Thu Mar 28 13:12:50 2013 From: Wanda.Smith <@t> HCAhealthcare.com (Wanda.Smith@HCAhealthcare.com) Date: Thu Mar 28 13:13:36 2013 Subject: [Histonet] List of Specimens a PA can Gross Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BA27D5E44DEA@NADCWPMSGCMS03.hca.corpad.net> Hello All, CAP IS HERE AS I TYPE THIS, and I am looking for a copy of the policy regarding what specimens a PA can gross with and without direct supervision. Will someone share theirs with me????? I am aware every lab is different, but it keep me from being cited! Thanks, Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. From lblazek <@t> digestivespecialists.com Thu Mar 28 13:51:34 2013 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Thu Mar 28 13:53:25 2013 Subject: [Histonet] RE: Biocare Decloaker Nx Gen In-Reply-To: <959202AC61AEF942968646EC66E2BE3E407C11@CSHSMSGMBX05.CSMC.EDU> References: <959202AC61AEF942968646EC66E2BE3E407C11@CSHSMSGMBX05.CSMC.EDU> Message-ID: <5A2BD13465E061429D6455C8D6B40E39164EEB14E0@IBMB7Exchange.digestivespecialists.com> We have used the Decloaking Chamber for several years now and have had excellent results. The nicest thing is that there is consistency and reliability. Each run will be the same as the one previous and having the ability to use the USB stick for documentation of time, pressure and temperature makes record keeping really easy. We have had no complaints. The only con I can come up with is that it comes in a big box that is hard to store if you have to send it back for some reason. Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Digestive Specialists, Inc Phone: (937) 396-2623 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Swartwood, Steven J Sent: Thursday, March 28, 2013 1:40 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Biocare Decloaker Nx Gen Has anyone ever used the Biocare Decloaking Chamber Nx Gen for epitope unmasking (heat retrieval)? Also does anyone have any personal pros/cons with this instrument? We are thinking about getting one because of inconsistencies with our current method using a vegetable steamer. We use many different tissue types both animal and human. Also we use many different antibodies. Currently with the vegetable steamer we've used Citrate, EDTA, and Tris-EDTA combinations. We've used times from 30-60 minutes, and even tried 30 minutes x 2 or 40 minutes x 2, but we still see some inconsistent staining. To make sure it wasn't due to fixation vimentin staining was performed and shows even staining. It's just certain antibodies that are giving us trouble even from multiple different companies. Any advice would be greatly appreciated and very beneficial to our work pertaining to the instrument. Thanks again everyone have a great end of the week, Steven Swartwood HT(ASCP) steven.swartwood@cshs.org IMPORTANT WARNING: This message is intended for the use of the person or entity to which it is addressed and may contain information that is privileged and confidential, the disclosure of which is governed by applicable law. If the reader of this message is not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this information is STRICTLY PROHIBITED. If you have received this message in error, please notify us immediately by calling (310) 423-6428 and destroy the related message. Thank You for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BZIMMERM <@t> gru.edu Thu Mar 28 13:57:46 2013 From: BZIMMERM <@t> gru.edu (Zimmerman, Billie) Date: Thu Mar 28 13:57:52 2013 Subject: [Histonet] Updates on Jekyll Island...Procrastinators please read this ASAP Message-ID: <7B3DEB32E69C034EACB479059C5DE3FF75EDBA@EX-MLB-03.ad.georgiahealth.edu> If you haven't reserved your room for the upcoming GSH meeting next month, please note that all island and standard Oceanside rooms have been reserved. But, just because you snoozed, were waiting on money for your institution, or you're just a classic procrastinator, there are still options. There are upgraded rooms such as the lanai. This room has a Jacuzzi, microwave, King size bed, small refrigerator, and a private balcony or patio. There's also the efficiency which has the kitchenette with a stove top, refrigerator, two double beds, sitting area, and private balcony or patio. The symposium rate still applies and if you have issues or concerns, contact Linda Schepps at Oceanside. She can't cure procrastination but she can help secure a room for you. While you're strolling around the historic area of the island in your speedo or crochet bikini, check out Becky's famous chicken salad. It's a little place with outside tables. Just walk up to the window and place your order. I attempted to have a chicken salad sandwich there but they were all sold out!! The lady managed to let me sample a teaspoon of it. It was delicious and didn't taste like that stuff in the grocery store. I plan to attempt another order when I return next month!! Augusta State University and Georgia Health Sciences University have consolidated to become Georgia Regents University. Effective January 9, 2013, my email address has changed to BZIMMERM@gru.edu. Please update your address book to reflect this change. From ratliffjack <@t> hotmail.com Thu Mar 28 14:16:45 2013 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Thu Mar 28 14:16:50 2013 Subject: [Histonet] Updates on Jekyll Island...Procrastinators please read this ASAP In-Reply-To: <7B3DEB32E69C034EACB479059C5DE3FF75EDBA@EX-MLB-03.ad.georgiahealth.edu> References: <7B3DEB32E69C034EACB479059C5DE3FF75EDBA@EX-MLB-03.ad.georgiahealth.edu> Message-ID: PLEASE! PLEASE! PLEASE! I want that room! Oh and since you mentioned speedos and crochet bikinis, you want me to bring my white mankini with the British flag on the front? Just kidding, I think I had better leave that at home! ? Jack Jack L. RatliffOwner/Histologist, Ratliff Histology Consultants, LLCChairman, Hard Tissue Committee - National Society for Histotechnology 389 Nichol Mill LaneFranklin, TN 37067(317) 281-1975 (c)(615) 236-4901 (o)(615) 236-4962 (f)jratliff@ratliffhistology.com > From: BZIMMERM@gru.edu > To: histonet@lists.utsouthwestern.edu > Date: Thu, 28 Mar 2013 18:57:46 +0000 > Subject: [Histonet] Updates on Jekyll Island...Procrastinators please read this ASAP > > If you haven't reserved your room for the upcoming GSH meeting next month, please note that all island and standard Oceanside rooms have been reserved. But, just because you snoozed, were waiting on money for your institution, or you're just a classic procrastinator, there are still options. There are upgraded rooms such as the lanai. This room has a Jacuzzi, microwave, King size bed, small refrigerator, and a private balcony or patio. There's also the efficiency which has the kitchenette with a stove top, refrigerator, two double beds, sitting area, and private balcony or patio. The symposium rate still applies and if you have issues or concerns, contact Linda Schepps at Oceanside. She can't cure procrastination but she can help secure a room for you. > > While you're strolling around the historic area of the island in your speedo or crochet bikini, check out Becky's famous chicken salad. It's a little place with outside tables. Just walk up to the window and place your order. I attempted to have a chicken salad sandwich there but they were all sold out!! The lady managed to let me sample a teaspoon of it. It was delicious and didn't taste like that stuff in the grocery store. I plan to attempt another order when I return next month!! > > > Augusta State University and Georgia Health Sciences University have consolidated to become Georgia Regents University. Effective January 9, 2013, my email address has changed to BZIMMERM@gru.edu. Please update your address book to reflect this change. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tfountain <@t> dsmanitoba.ca Thu Mar 28 14:36:11 2013 From: tfountain <@t> dsmanitoba.ca (Tiana Baskin) Date: Thu Mar 28 14:36:16 2013 Subject: [Histonet] RE: Biocare Decloaker Nx Gen In-Reply-To: <959202AC61AEF942968646EC66E2BE3E407C11@CSHSMSGMBX05.CSMC.EDU> References: <959202AC61AEF942968646EC66E2BE3E407C11@CSHSMSGMBX05.CSMC.EDU> Message-ID: I have had the pleasure of seeing a demo of these Nxgen decloakers and I was very impressed. I currently use the older generation ones which still work very reliably for me. There is a nice tray inside the chamber to ensure the slide containers do not tip over and the USB which tracks all of the temperature and pressure information is a QC dream. The only thing which I could see as a down side would be the programming. It is preset from what I remember so you have to choose from the programs already existing. I think the existing programs should cover most antigens but if you wanted to be creative you don't have that option. I would love to have one in my lab ... Enjoy -------------- next part -------------- This email and/or any documents in this transmission is intended for the addressee(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original. Ce courriel et tout document dans cette transmission est destin? ? la personne ou aux personnes ? qui il est adress?. Il peut contenir des informations privil?gi?es ou confidentielles. Toute utilisation, divulgation, distribution, copie, ou diffusion non autoris?e est strictement d?fendue. Si vous n'?tes pas le destinataire de ce message, veuillez en informer l'exp?diteur imm?diatement et lui remettre l'original. From pinkladyjmb <@t> yahoo.com Thu Mar 28 14:03:14 2013 From: pinkladyjmb <@t> yahoo.com (Jennifer Blanchard) Date: Thu Mar 28 14:57:36 2013 Subject: [Histonet] (no subject) Message-ID: <1364497394.56352.YahooMailNeo@web142501.mail.bf1.yahoo.com> http://www.savvybusinessmakers.com/iseiy/pqih.yxdxy?eglxo 3/28/2013 8:03:09 PM 3/28/2013 8:03:09 PM From Wanda.Smith <@t> HCAhealthcare.com Thu Mar 28 15:07:03 2013 From: Wanda.Smith <@t> HCAhealthcare.com (Wanda.Smith@HCAhealthcare.com) Date: Thu Mar 28 15:07:39 2013 Subject: [Histonet] Thanks for your help on PA Grossing Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BA27D5E45069@NADCWPMSGCMS03.hca.corpad.net> Thanks to ALL! I have what I need!!! Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. From POWELL_SA <@t> mercer.edu Thu Mar 28 15:15:51 2013 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Thu Mar 28 15:15:55 2013 Subject: [Histonet] Updates on Jekyll Island In-Reply-To: References: <7B3DEB32E69C034EACB479059C5DE3FF75EDBA@EX-MLB-03.ad.georgiahealth.edu> Message-ID: <9BF995BC0E47744E9673A41486E24EE25797C068A6@MERCERMAIL.MercerU.local> Waaaaaaaaaaaaaaaaaaay too much information Jack, just keep it covered. You can bring all those wonderful photos of your European trip with your Dad. I do hope you have made your reservations at Jekyll. Sounds like the rooms are going fast. See you at the beach. Oh and Jack, they will arrest you if you show too much. This is Georgia you know. Sp -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jack Ratliff Sent: Thursday, March 28, 2013 3:17 PM To: Zimmerman, Billie; Histonet Subject: RE: [Histonet] Updates on Jekyll Island...Procrastinators please read this ASAP PLEASE! PLEASE! PLEASE! I want that room! Oh and since you mentioned speedos and crochet bikinis, you want me to bring my white mankini with the British flag on the front? Just kidding, I think I had better leave that at home! ? Jack Jack L. RatliffOwner/Histologist, Ratliff Histology Consultants, LLCChairman, Hard Tissue Committee - National Society for Histotechnology 389 Nichol Mill LaneFranklin, TN 37067(317) 281-1975 (c)(615) 236-4901 (o)(615) 236-4962 (f)jratliff@ratliffhistology.com > From: BZIMMERM@gru.edu > To: histonet@lists.utsouthwestern.edu > Date: Thu, 28 Mar 2013 18:57:46 +0000 > Subject: [Histonet] Updates on Jekyll Island...Procrastinators please read this ASAP > > If you haven't reserved your room for the upcoming GSH meeting next month, please note that all island and standard Oceanside rooms have been reserved. But, just because you snoozed, were waiting on money for your institution, or you're just a classic procrastinator, there are still options. There are upgraded rooms such as the lanai. This room has a Jacuzzi, microwave, King size bed, small refrigerator, and a private balcony or patio. There's also the efficiency which has the kitchenette with a stove top, refrigerator, two double beds, sitting area, and private balcony or patio. The symposium rate still applies and if you have issues or concerns, contact Linda Schepps at Oceanside. She can't cure procrastination but she can help secure a room for you. > > While you're strolling around the historic area of the island in your speedo or crochet bikini, check out Becky's famous chicken salad. It's a little place with outside tables. Just walk up to the window and place your order. I attempted to have a chicken salad sandwich there but they were all sold out!! The lady managed to let me sample a teaspoon of it. It was delicious and didn't taste like that stuff in the grocery store. I plan to attempt another order when I return next month!! > > > Augusta State University and Georgia Health Sciences University have consolidated to become Georgia Regents University. Effective January 9, 2013, my email address has changed to BZIMMERM@gru.edu. Please update your address book to reflect this change. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ratliffjack <@t> hotmail.com Thu Mar 28 17:06:07 2013 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Thu Mar 28 17:06:15 2013 Subject: [Histonet] RE: Updates on Jekyll Island In-Reply-To: <9BF995BC0E47744E9673A41486E24EE25797C068A6@MERCERMAIL.MercerU.local> References: <7B3DEB32E69C034EACB479059C5DE3FF75EDBA@EX-MLB-03.ad.georgiahealth.edu>, , <9BF995BC0E47744E9673A41486E24EE25797C068A6@MERCERMAIL.MercerU.local> Message-ID: Im not that crazy Shirley, but I have worn a speedo on the beach within the last 8 months just for fun! LOL Yes, I have my reservation and I believe it is ocean/beach side, but that jacuzzi is a tempting thought to change! My Dad always says that only a fool wouldn't change their mind! LOL One more thing, if the weather is nice during those days, I'm seriously thinking of riding my Harley out there! See you all very soon! Jack > From: POWELL_SA@mercer.edu > To: ratliffjack@hotmail.com; bzimmerm@gru.edu; histonet@lists.utsouthwestern.edu > Date: Thu, 28 Mar 2013 16:15:51 -0400 > Subject: Updates on Jekyll Island > > Waaaaaaaaaaaaaaaaaaay too much information Jack, just keep it covered. You can bring all those wonderful photos of your European trip with your Dad. I do hope you have made your reservations at Jekyll. Sounds like the rooms are going fast. > > See you at the beach. Oh and Jack, they will arrest you if you show too much. This is Georgia you know. > > Sp > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jack Ratliff > Sent: Thursday, March 28, 2013 3:17 PM > To: Zimmerman, Billie; Histonet > Subject: RE: [Histonet] Updates on Jekyll Island...Procrastinators please read this ASAP > > PLEASE! PLEASE! PLEASE! I want that room! > Oh and since you mentioned speedos and crochet bikinis, you want me to bring my white mankini with the British flag on the front? Just kidding, I think I had better leave that at home! ? > Jack > > Jack L. RatliffOwner/Histologist, Ratliff Histology Consultants, LLCChairman, Hard Tissue Committee - National Society for Histotechnology > 389 Nichol Mill LaneFranklin, TN 37067(317) 281-1975 (c)(615) 236-4901 (o)(615) 236-4962 (f)jratliff@ratliffhistology.com > > > > From: BZIMMERM@gru.edu > > To: histonet@lists.utsouthwestern.edu > > Date: Thu, 28 Mar 2013 18:57:46 +0000 > > Subject: [Histonet] Updates on Jekyll Island...Procrastinators please read this ASAP > > > > If you haven't reserved your room for the upcoming GSH meeting next month, please note that all island and standard Oceanside rooms have been reserved. But, just because you snoozed, were waiting on money for your institution, or you're just a classic procrastinator, there are still options. There are upgraded rooms such as the lanai. This room has a Jacuzzi, microwave, King size bed, small refrigerator, and a private balcony or patio. There's also the efficiency which has the kitchenette with a stove top, refrigerator, two double beds, sitting area, and private balcony or patio. The symposium rate still applies and if you have issues or concerns, contact Linda Schepps at Oceanside. She can't cure procrastination but she can help secure a room for you. > > > > While you're strolling around the historic area of the island in your speedo or crochet bikini, check out Becky's famous chicken salad. It's a little place with outside tables. Just walk up to the window and place your order. I attempted to have a chicken salad sandwich there but they were all sold out!! The lady managed to let me sample a teaspoon of it. It was delicious and didn't taste like that stuff in the grocery store. I plan to attempt another order when I return next month!! > > > > > > Augusta State University and Georgia Health Sciences University have consolidated to become Georgia Regents University. Effective January 9, 2013, my email address has changed to BZIMMERM@gru.edu. Please update your address book to reflect this change. > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From badzrosari <@t> yahoo.com Fri Mar 29 05:21:42 2013 From: badzrosari <@t> yahoo.com (Bernadette del Rosario) Date: Fri Mar 29 05:21:51 2013 Subject: [Histonet] Lymphnode retrieval solution Message-ID: <1364552502.97653.YahooMailNeo@web121501.mail.ne1.yahoo.com> Has anyone there can share the best solution for retrieval of lymphnodes in the fats.Other than the GEWF/Penfix solutions,has anyone used a xylene based solution which will dissolved the fats making it transparent looking?and for lymphnodes to be seen easily?Cant find anything in the google..Highly appreciate any methods. From badzrosari <@t> yahoo.com Fri Mar 29 05:25:21 2013 From: badzrosari <@t> yahoo.com (Bernadette del Rosario) Date: Fri Mar 29 05:25:31 2013 Subject: [Histonet] Lymphnode retrieval solution Message-ID: <1364552721.26522.YahooMailNeo@web121502.mail.ne1.yahoo.com> Has anyone there can share the best solution for retrieval of lymphnodes in the fats.Other than the GEWF/Penfix solutions,has anyone used a xylene based solution which will dissolved the fats making it transparent ?and for lymphnodes to be seen easily.Any method? highly appreciated. From ttruscot <@t> vetmed.wsu.edu Fri Mar 29 09:33:12 2013 From: ttruscot <@t> vetmed.wsu.edu (Truscott, Tom) Date: Fri Mar 29 09:33:21 2013 Subject: [Histonet] Lymphnode retrieval solution In-Reply-To: <1364552502.97653.YahooMailNeo@web121501.mail.ne1.yahoo.com> References: <1364552502.97653.YahooMailNeo@web121501.mail.ne1.yahoo.com> Message-ID: <9EF5279EBDFE6E4FB6605E8F183A00274B622C53@CVM76.vetmed.wsu.edu> Hi Bernadette, Have you tried soaking the tissue in white vinegar? Tom Truscott -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernadette del Rosario Sent: Friday, March 29, 2013 3:22 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Lymphnode retrieval solution Has anyone there can share the best solution for retrieval of lymphnodes in the fats.Other than the GEWF/Penfix solutions,has anyone used a xylene based solution which will dissolved the fats making it transparent looking?and for lymphnodes to be seen easily?Cant find anything in the google..Highly appreciate any methods. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Fri Mar 29 10:16:17 2013 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Fri Mar 29 10:16:31 2013 Subject: [Histonet] minus 80 freezer safe time in power outage Message-ID: <761E2B5697F795489C8710BCC72141FF06837A@ex07.net.ucsf.edu> Hi all, For a minus 80 freezer what would you consider a "safe" time period that it can go without power before the temperature gets too high and contents need to be transferred (assuming door stays closed)? I'm thinking -40 would be a cutoff temp to move samples. Anyone else think differently about that? Thanks for any info! Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center 505 Parnassus Ave, Box 1656 Room S570 San Francisco, CA 94143 (415) 353-1266 (ph) (415) 514-3403 (fax) tim.morken@ucsfmedctr.org From doug.porter <@t> caplab.org Fri Mar 29 10:41:50 2013 From: doug.porter <@t> caplab.org (Douglas Porter) Date: Fri Mar 29 10:37:42 2013 Subject: [Histonet] Lymph node retrieval solution Message-ID: <001101ce2c93$eeca67e0$cc5f37a0$@caplab.org> We use a 50/50 solution of Acetone and Alcohol. It renders the fat somewhat transparent and turns the lymph nodes white. Keep in mind you need to fix the specimen properly BEFORE you use this technique. You will also need to use 3 to 4 changes at about 15 minutes each. Separate, pull apart, the bigger pieces to get better penetration of the solution and stir after first five minutes or so. I routinely find 1mm lymph nodes this way. Happy hunting! Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.porter@caplab.org www.caplab.org ? ? The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, copying, forwarding or capture of this communication is strictly prohibited. If you have received this communication in error, please notify me immediately by return e-mail and delete this and all copies. Thank-you. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Truscott, Tom Sent: Friday, March 29, 2013 10:33 AM To: Bernadette del Rosario; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Lymphnode retrieval solution Hi Bernadette, Have you tried soaking the tissue in white vinegar? Tom Truscott -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernadette del Rosario Sent: Friday, March 29, 2013 3:22 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Lymphnode retrieval solution Has anyone there can share the best solution for retrieval of lymphnodes in the fats.Other than the GEWF/Penfix solutions,has anyone used a xylene based solution which will dissolved the fats making it transparent looking?and for lymphnodes to be seen easily?Cant find anything in the google..Highly appreciate any methods. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMitchell <@t> uwhealth.org Fri Mar 29 10:38:17 2013 From: JMitchell <@t> uwhealth.org (Mitchell Jean A) Date: Fri Mar 29 10:38:23 2013 Subject: [Histonet] RE: minus 80 freezer safe time in power outage In-Reply-To: <761E2B5697F795489C8710BCC72141FF06837A@ex07.net.ucsf.edu> References: <761E2B5697F795489C8710BCC72141FF06837A@ex07.net.ucsf.edu> Message-ID: <16F90B93CA23D446980B3D591FD02DAD04E127@UWHC-MBX14.uwhis.hosp.wisc.edu> Tim: speaking from experience (unfortunately) and the failure of a -80/-70 freezer. As you would imagine once the power goes out or the freezer malfunctions - the freezer temperature rises very quickly. I would estimate, and without opening the door, at the most you have 1-2 hours before you are at -40 and or higher. But if the freezer malfunctions - you usually open the door to check for any ice blockage/build-up that might be causing a poor seal and once you open the door the temp increases very quickly. When these ultra-low freezers fail you don't have much time to transfer tissue - and usually in the middle of the night....... Jean Mitchell, BS HT (ASCP) University of Wisconsin Hospital & Clinics Neuromuscular Laboratory 600 Highland Avenue Madison, WI 53792-5132 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Friday, March 29, 2013 10:16 AM To: Histonet Subject: [Histonet] minus 80 freezer safe time in power outage Hi all, For a minus 80 freezer what would you consider a "safe" time period that it can go without power before the temperature gets too high and contents need to be transferred (assuming door stays closed)? I'm thinking -40 would be a cutoff temp to move samples. Anyone else think differently about that? Thanks for any info! Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center 505 Parnassus Ave, Box 1656 Room S570 San Francisco, CA 94143 (415) 353-1266 (ph) (415) 514-3403 (fax) tim.morken@ucsfmedctr.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hfedor <@t> jhmi.edu Fri Mar 29 11:19:05 2013 From: hfedor <@t> jhmi.edu (Helen Fedor) Date: Fri Mar 29 11:19:37 2013 Subject: [Histonet] RE: minus 80 freezer safe time in power outage In-Reply-To: <761E2B5697F795489C8710BCC72141FF06837A@ex07.net.ucsf.edu> References: <761E2B5697F795489C8710BCC72141FF06837A@ex07.net.ucsf.edu> Message-ID: Hello, I think it would depend on what you think the malfunction is due to. If you do not have a solution in sight. I would start thinking about moving them immediately. RNA is sensitive to this type of warming. How long is the transfer going to take? If they already up to -40 they are going to be to -30 when they get to their new destination. And that is a problem. Helen -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Friday, March 29, 2013 11:16 AM To: Histonet Subject: [Histonet] minus 80 freezer safe time in power outage Hi all, For a minus 80 freezer what would you consider a "safe" time period that it can go without power before the temperature gets too high and contents need to be transferred (assuming door stays closed)? I'm thinking -40 would be a cutoff temp to move samples. Anyone else think differently about that? Thanks for any info! Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center 505 Parnassus Ave, Box 1656 Room S570 San Francisco, CA 94143 (415) 353-1266 (ph) (415) 514-3403 (fax) tim.morken@ucsfmedctr.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Pathrm35 <@t> comcast.net Fri Mar 29 11:29:56 2013 From: Pathrm35 <@t> comcast.net (Pathrm35@comcast.net) Date: Fri Mar 29 11:30:29 2013 Subject: [Histonet] Sakura service plans Message-ID: <309781336.236580.1364574596514.JavaMail.root@sz0123a.westchester.pa.mail.comcast.net> I was wondering how many of you are using/paying for the service plans for the Sakura/Tissue Tek tissue processor, embedding center and Prisma coverslipper/stainer combo? Are these service plans worth the money? Thanks, Ron From liz <@t> premierlab.com Fri Mar 29 11:35:10 2013 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Fri Mar 29 11:35:13 2013 Subject: [Histonet] Sakura service plans In-Reply-To: <309781336.236580.1364574596514.JavaMail.root@sz0123a.westchester.pa.mail.comcast.net> References: <309781336.236580.1364574596514.JavaMail.root@sz0123a.westchester.pa.mail.comcast.net> Message-ID: <14E2C6176416974295479C64A11CB9AE016411AC299E@SBS2K8.premierlab.local> Ron We use a local service that has been trained in PM's on Sakura. We priced out the Sakura service and it was significantly more than the local service company that we use. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Laboratory Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 Work (303) 682-3949 Fax (303) 682-9060 Cell (303) 881-0763 liz@premierlab.com www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pathrm35@comcast.net Sent: Friday, March 29, 2013 10:30 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sakura service plans I was wondering how many of you are using/paying for the service plans for the Sakura/Tissue Tek tissue processor, embedding center and Prisma coverslipper/stainer combo? Are these service plans worth the money? Thanks, Ron _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mjdessoye <@t> commonwealthhealth.net Fri Mar 29 11:41:23 2013 From: mjdessoye <@t> commonwealthhealth.net (Dessoye, Michael J) Date: Fri Mar 29 11:41:35 2013 Subject: [Histonet] Cell block preparation Message-ID: Hello Histonet, What is everyone doing regarding cell block preparation? We have been using sponges for processing but we've had a recent contamination issue that may be attributable to the sponges (fluid types vary but mostly pleural fluids). We will be testing sponges, biopsy bags, and paper... anyone have a method they prefer? Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdessoye@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Commonwealth Health. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. From Valerie.Hannen <@t> parrishmed.com Fri Mar 29 11:52:34 2013 From: Valerie.Hannen <@t> parrishmed.com (Hannen, Valerie) Date: Fri Mar 29 11:52:38 2013 Subject: [Histonet] RE: Cell block preparation In-Reply-To: Message-ID: <450B7A81EDA0C54E97C53D60F00776C3231B3D7C14@isexstore03> We wrap our fluid blocks in paper, and I have not had a problem with cross-contamination. Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.hannen@parrishmed.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dessoye, Michael J Sent: Friday, March 29, 2013 12:41 PM To: Histonet Subject: [Histonet] Cell block preparation Hello Histonet, What is everyone doing regarding cell block preparation? We have been using sponges for processing but we've had a recent contamination issue that may be attributable to the sponges (fluid types vary but mostly pleural fluids). We will be testing sponges, biopsy bags, and paper... anyone have a method they prefer? Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdessoye@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Commonwealth Health. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet =================== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" =================== From Timothy.Morken <@t> ucsfmedctr.org Fri Mar 29 11:55:23 2013 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Fri Mar 29 11:55:36 2013 Subject: [Histonet] Sakura service plans In-Reply-To: <309781336.236580.1364574596514.JavaMail.root@sz0123a.westchester.pa.mail.comcast.net> References: <309781336.236580.1364574596514.JavaMail.root@sz0123a.westchester.pa.mail.comcast.net> Message-ID: <761E2B5697F795489C8710BCC72141FF068406@ex07.net.ucsf.edu> Ron, a few considerations" 1) Is there a company in your local area that can do it cheaper? (sakura contracts out the service, so that company may do it cheaper than going thru sakura) 2) Can they be there fast if you need it? At least as fast as the Sakura contractor 3) If you are thinking of going the PO route for each service and PM, a single service will cost almost half the price of an annual priority-service contract that includes a PM. 4) do you have a biomed department that can be trained to do the service? Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pathrm35@comcast.net Sent: Friday, March 29, 2013 9:30 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sakura service plans I was wondering how many of you are using/paying for the service plans for the Sakura/Tissue Tek tissue processor, embedding center and Prisma coverslipper/stainer combo? Are these service plans worth the money? Thanks, Ron _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TMcNemar <@t> lmhealth.org Fri Mar 29 11:59:51 2013 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Fri Mar 29 11:58:56 2013 Subject: [Histonet] RE: Cell block preparation In-Reply-To: References: Message-ID: We wrap them in lens paper. No problems. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dessoye, Michael J Sent: Friday, March 29, 2013 12:41 PM To: Histonet Subject: [Histonet] Cell block preparation Hello Histonet, What is everyone doing regarding cell block preparation? We have been using sponges for processing but we've had a recent contamination issue that may be attributable to the sponges (fluid types vary but mostly pleural fluids). We will be testing sponges, biopsy bags, and paper... anyone have a method they prefer? Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdessoye@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Commonwealth Health. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From jqb7 <@t> cdc.gov Fri Mar 29 12:01:21 2013 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/OID/NCEZID)) Date: Fri Mar 29 12:01:47 2013 Subject: [Histonet] Sakura service plans In-Reply-To: <309781336.236580.1364574596514.JavaMail.root@sz0123a.westchester.pa.mail.comcast.net> References: <309781336.236580.1364574596514.JavaMail.root@sz0123a.westchester.pa.mail.comcast.net> Message-ID: We use Sakura for our VIP-%'s and VIP-6 as well as our Prisma and Xpress and AutoTEC (this is the automatic embedder, not the traditional embedding center). We find it is worth the extra money as we get continuous free software upgrades that independent provider s do not offer. Jeanine H. Bartlett Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 404-639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pathrm35@comcast.net Sent: Friday, March 29, 2013 12:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sakura service plans I was wondering how many of you are using/paying for the service plans for the Sakura/Tissue Tek tissue processor, embedding center and Prisma coverslipper/stainer combo? Are these service plans worth the money? Thanks, Ron _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From steve8438 <@t> gmail.com Fri Mar 29 12:02:39 2013 From: steve8438 <@t> gmail.com (Steve Mello) Date: Fri Mar 29 12:02:47 2013 Subject: [Histonet] RE: Cell block preparation In-Reply-To: References: Message-ID: <1171931536-1364576559-cardhu_decombobulator_blackberry.rim.net-532327378-@b12.c1.bise6.blackberry> Lens paper has always been the best! Steve Mello HT(ASCP) Sent via BlackBerry by AT&T -----Original Message----- From: Tom McNemar Sender: histonet-bounces@lists.utsouthwestern.edu Date: Fri, 29 Mar 2013 12:59:51 To: 'Dessoye, Michael J'; Histonet Subject: [Histonet] RE: Cell block preparation We wrap them in lens paper. No problems. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dessoye, Michael J Sent: Friday, March 29, 2013 12:41 PM To: Histonet Subject: [Histonet] Cell block preparation Hello Histonet, What is everyone doing regarding cell block preparation? We have been using sponges for processing but we've had a recent contamination issue that may be attributable to the sponges (fluid types vary but mostly pleural fluids). We will be testing sponges, biopsy bags, and paper... anyone have a method they prefer? Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdessoye@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Commonwealth Health. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From plambert <@t> wisc.edu Fri Mar 29 12:10:00 2013 From: plambert <@t> wisc.edu (Paul Lambert) Date: Fri Mar 29 12:10:12 2013 Subject: [Histonet] please remove me from your email list Message-ID: <5D7F3100-1908-4A59-A1F6-A26EEDBE8A83@wisc.edu> please remove me from your email list. Thanks, Paul Paul F. Lambert, Ph.D. Howard M Temin Professor of Oncology McArdle Laboratory for Cancer Research University of Wisconsin School of Medicine and Public Health 1400 University Ave. Madison WI 53706 USA tel: 608-262-8533 fax: 608-262-2824 email: plambert@wisc.edu From dean1252 <@t> yahoo.com Fri Mar 29 15:39:00 2013 From: dean1252 <@t> yahoo.com (Nadine Abbott) Date: Fri Mar 29 15:39:08 2013 Subject: [Histonet] Genta vs alcian blue Message-ID: <1364589540.49615.YahooMailNeo@web121402.mail.ne1.yahoo.com> Hello Eveyone, ? My pathologist has asked me to find out what is the difference in the alcian blue stain vs the genta stain in assisting in the diagnosis of signet ring cell carcinoma. Any assistance you can give me would be much appreciated. ? Thanks so much. ? Nadine Abbott, HTL Grande Ronde Hospital La Grande, Oregon From Bruce_Palmatier <@t> vwr.com Fri Mar 29 15:01:44 2013 From: Bruce_Palmatier <@t> vwr.com (Bruce_Palmatier@vwr.com) Date: Fri Mar 29 15:39:46 2013 Subject: [Histonet] AUTO: Bruce Palmatier is out of the office (returning 04/02/2013) Message-ID: I am out of the office until 04/02/2013. I will be out of the office from March 29th through April 1st. If you have a question about an order or need assistance placing an order, or if the matter is urgent, please call 877-881-1192 between 8am and 8pm and a VWR Healthcare Service associate will assist you. For quote requests and pricing-related inquiries, I will respond with 24 hours. Thank You, Bruce Palmatier VWR Healthcare mobile 484-319-5563 Note: This is an automated response to your message "Histonet Digest, Vol 112, Issue 32" sent on 3/29/2013 12:20:58 PM. This is the only notification you will receive while this person is away. From amber.mckenzie <@t> gastrodocs.net Fri Mar 29 16:39:21 2013 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Fri Mar 29 16:49:44 2013 Subject: [Histonet] Flow cytometry protocol In-Reply-To: <455897B94CF3A44F81F727160F23FB110C7F7927@DC229.ad.dawsoncollege.qc.ca> References: <455897B94CF3A44F81F727160F23FB110C7E996E@DC229.ad.dawsoncollege.qc.ca>, <455897B94CF3A44F81F727160F23FB110C7F7927@DC229.ad.dawsoncollege.qc.ca> Message-ID: <5A33C952BB67F4468AF1F36D739212BCBCEB39A8@JERRY.Gia.com> Does anyone have a flow cytometry protocol they'd like to share? From Joyce.Weems <@t> emoryhealthcare.org Fri Mar 29 16:52:50 2013 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Fri Mar 29 16:53:01 2013 Subject: [Histonet] Genta vs alcian blue In-Reply-To: <1364589540.49615.YahooMailNeo@web121402.mail.ne1.yahoo.com> References: <1364589540.49615.YahooMailNeo@web121402.mail.ne1.yahoo.com> Message-ID: We have used the Genta for Helicobacter. It is a modified Steiner - with Alcian blue at the end - and then Hematoxylin and eosin. It is easy to see H. pylori as well as the morphology. I don't know how it relates to signet ring ca though. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nadine Abbott Sent: Friday, March 29, 2013 4:39 PM To: histonet Subject: [Histonet] Genta vs alcian blue Hello Eveyone, My pathologist has asked me to find out what is the difference in the alcian blue stain vs the genta stain in assisting in the diagnosis of signet ring cell carcinoma. Any assistance you can give me would be much appreciated. Thanks so much. Nadine Abbott, HTL Grande Ronde Hospital La Grande, Oregon _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From rllott <@t> bellsouth.net Sat Mar 30 16:27:17 2013 From: rllott <@t> bellsouth.net (Robert Lott) Date: Sat Mar 30 16:28:04 2013 Subject: [Histonet] Genta vs alcian blue Message-ID: <000001ce2d8d$5b20d4c0$11627e40$@net> As Joyce has pointed out below, the Genta is a Modified Steiner (for visualization of H.P.) then Alcian Blue (for visualization of signet ring cells and any other metaplasia) and H&E (for visualization of routine gastric morphology)... J Clin Pathol. 1997 October; 50(10): 867-868. PMCID: PMC500272 Early diagnosis of signet ring cell carcinoma of the stomach: role of the Genta stain. H M el-Zimaity, K Itani, and D Y Graham Department of Medicine, Veterans Affairs Medical Center, Houston, Texas, USA. Copyright notice Abstract Signet ring cell carcinoma is a poorly differentiated adenocarcinoma in which the tumor cells invade singly or in small groups. Early stages of the disease can be missed easily when using regular hematoxylin and eosin staining. This is a report of a case in which routine screening of gastric biopsies with the Genta stain was responsible for rapid identification of signet ring carcinoma. The patient, a 29 year old woman, had a large portion of the antrum excised surgically for signet ring cell gastric carcinoma. Follow up endoscopy six years later showed no evidence of tumor. Twenty six large cup biopsies were obtained and a "single focus" of signet ring tumor cells infiltrating the surface mucosa in single file was seen. The diagnosis was missed on hematoxylin and eosin stain by three senior pathologists but owing to "the Alcian blue component" of the Genta stain the tumor cells were recognized easily. Thus, the Genta stain not only facilitates "detection of Helicobacter pylori" but also allows for "simultaneous visualization of gastric morphology" as well as "signet ring carcinoma" that can be missed with conventional stains. Best Regards, Robert L. Lott Robert L. Lott, HTL(ASCP) rllott@bellsouth.net 205-746-5628 Message: 7 Date: Fri, 29 Mar 2013 21:52:50 +0000 From: "Weems, Joyce K." Subject: RE: [Histonet] Genta vs alcian blue To: "'Nadine Abbott'" , histonet Message-ID: Content-Type: text/plain; charset="iso-8859-1" We have used the Genta for Helicobacter. It is a modified Steiner - with Alcian blue at the end - and then Hematoxylin and eosin. It is easy to see H. pylori as well as the morphology. I don't know how it relates to signet ring ca though. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nadine Abbott Sent: Friday, March 29, 2013 4:39 PM To: histonet Subject: [Histonet] Genta vs alcian blue Hello Eveyone, My pathologist has asked me to find out what is the difference in the alcian blue stain vs the genta stain in assisting in the diagnosis of signet ring cell carcinoma. Any assistance you can give me would be much appreciated. Thanks so much. Nadine Abbott, HTL Grande Ronde Hospital La Grande, Oregon _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lins0701 <@t> gmail.com Sat Mar 30 21:08:40 2013 From: lins0701 <@t> gmail.com (Sophia Lin) Date: Sat Mar 30 21:08:50 2013 Subject: [Histonet] Cell blocks Message-ID: When we did cell blocks we used agar after the slides were prepared for cytospins. We noticed that the addition of agar to the cell blocks (in the centrifuge tubes) made it easier for the techs to cut. And wrapped it in lens paper. They came out consistent each time. Hope this helps! Sent from my iPhone From SteveM <@t> mcclainlab.com Sun Mar 31 05:44:06 2013 From: SteveM <@t> mcclainlab.com (Steve McClain) Date: Sun Mar 31 05:44:21 2013 Subject: [Histonet] RE: Histonet Digest, Vol 112, Issue 34 genta stain Message-ID: <012ADA4B5CC00F4AB5E4BAA399E0A5DF336A3779@ML1.McClainLabs.local> May well work-Genta stain has AlcianBlue. Please note HL Steedman's original paper describes "formaldehyde fixative is not good" for stabilizing/fixing mucin. Steve A. McClain, MD 631 361 4000