[Histonet] Re: Histonet Digest, Vol 115, Issue 32

Barr,Kaye H khbarr <@t> mdanderson.org
Sat Jun 29 12:17:03 CDT 2013



Sent from my iPad

On Jun 29, 2013, at 12:03 PM, "histonet-request <@t> lists.utsouthwestern.edu" <histonet-request <@t> lists.utsouthwestern.edu> wrote:

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> Today's Topics:
> 
>   1. Decalcifier, adding Zinc Fixative (M.O.)
>   2. RE: Disposable blade information (Tom McNemar)
>   3. RE: Dragon users  (Gray, Ed)
>   4. PowerPath Issues (Willis, Donna G.)
>   5. Re: PowerPath Issues (Jesus Ellin)
>   6. Alport Syndrome (CHRISTIE GOWAN)
>   7. antibody providers for cytokine/chemokine IHC (Nathan Ostlie (S))
> 
> 
> ----------------------------------------------------------------------
> 
> Message: 1
> Date: Fri, 28 Jun 2013 11:09:04 -0700
> From: "M.O." <modz9636 <@t> gmail.com>
> Subject: [Histonet] Decalcifier, adding Zinc Fixative
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
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> 
> Hello Histonet!  Would anyone know if it is okay to add zinc fixative to
> TBD-2 decalcifier?  What ratio would be okay?
> 
> I am having an issue with human bone samples and want to make sure that
> these bones are properly fixed.  They are about 7mm thick and I am fixing
> them for 4 days, but additional fixation in the TBD-2 would be best.
> 
> Thank you,
> Merissa
> 
> 
> ------------------------------
> 
> Message: 2
> Date: Fri, 28 Jun 2013 14:16:29 -0400
> From: Tom McNemar <TMcNemar <@t> lmhealth.org>
> Subject: [Histonet] RE: Disposable blade information
> To: "'Hannen, Valerie'" <Valerie.Hannen <@t> parrishmed.com>,
>    "'histonet <@t> lists.utsouthwestern.edu'"
>    <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
>    <E9A90E28259D2F4E84308C5E8EA8F7B40153AB2F8FB2 <@t> lmhs-exchange>
> Content-Type: text/plain; charset="us-ascii"
> 
> I didn't think anybody still used steel knives....  I remember those days....
> 
> I would say to get away from the steel as soon as you can.  Disposable will make your life so much easier.
> 
> As you already know, it is near impossible to give you anything more than a guestimate but I would imagine that you could get by with one to three per day depending on case mix, staples, sutures, calcifications, etc.  There are so many variables.  It also depends on how many people are cutting those 50 blocks.
> 
> There are a lot blades to choose from.  You could probably get by for quite awhile just trying out different samples....
> 
> See if you can find a rep that can loan you a blade holder and try some.  The holder will be the biggest upfront expense.
> 
> I don't really know what the difference is between the high and low profiles but I suspect not much more than the height.
> 
> I have tried many different brands over the years and always go back to the AccuEdge low profile.
> 
> Tom McNemar, HT(ASCP)
> Histology Co-ordinator
> Licking Memorial Health Systems
> (740) 348-4163
> (740) 348-4166
> tmcnemar <@t> lmhealth.org
> www.LMHealth.org
> 
> 
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie
> Sent: Friday, June 28, 2013 12:03 PM
> To: 'histonet <@t> lists.utsouthwestern.edu'
> Subject: [Histonet] Disposable blade information
> 
> Good Morning all,
> 
> I am having to do a cost analysis study for the cost effectiveness of Sharpening steel blades vs. transitioning to disposable blades. I know...what you all are thinking..
> 
> "Your're still using steel blades??"...Yes, we are... :).  Our current knife sharpener has some issues, and I have decided to weigh buying a used knife sharpener against
> 
> switching to the disposable system.  So... a little background for you. We are a relatively small department, we average @ 50 blocks a day, of various tissues types.
> 
> My questions to you are this...and I know that answers will only be  estimations...but it is  a place for me to start.
> 
> What would be the average number of disposable blades that I would probably use in a day/ week?  On an average...how many blocks can be cut on each blade?
> 
> Has anyone recently made this transitiion? If so, what were some of the challenges??   I'm going to show my lack of knowledge of disposable blades with this next
> 
> question...but here it goes.. What is the difference between a low profile blade and a high profile blade...and would I need to buy both??
> 
> Ok... my head is hurting...so.. I am going to leave you with all the above questions.
> 
> Any and all information, suggestions, etc. will be greatly appreciated!!
> 
>   Thanks and have a GREAT day!!
> 
> Valerie A. Hannen, MLT(ASCP),HTL,SU(FL)
> Histology Section Chief
> Parrish Medical Center
> 951 N. Washington Ave.
> Titusville, Florida 32976
> Phone:(321) 268-6333 ext. 7506
> Fax: (321) 268-6149
> valerie.hannen <@t> parrishmed.com
> 
> 
> 
> 
> 
> 
> 
> 
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> This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you.
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> 
> 
> ------------------------------
> 
> Message: 3
> Date: Fri, 28 Jun 2013 14:15:21 -0400
> From: "Gray, Ed" <egray <@t> hsc.wvu.edu>
> Subject: [Histonet] RE: Dragon users 
> To: "histonet <@t> lists.utsouthwestern.edu"
>    <histonet <@t> lists.utsouthwestern.edu>
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>    <0BBA94171603A6468FFBA03A45A029FC04441004B7 <@t> MSEXCL.hscresex.local>
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> 
> We use Sennheiser RF sets in the gross room.  The sound quality is excellent and produce the best recognition of any wireless mike we've tried.  These were pretty much the only wireless option Nuance would recommend when we first started using Dragon several years ago. The cons are:
> 1.  The RF receiver is 21 x 10.5 x 3.5 cm with an external antenna and power supply, not huge but significant compared to a USB Bluetooth adapter.
> 2.  You are wired to a transmitter which is not terribly large or heavy but a wire to your belt/pocket none-the-less.
> 3.  They are battery hogs and quality drops rapidly once the indicator goes below 50%.
> 4.  They were expensive when we bought them.  I think they were over $300.00.  We bought some replacement headsets at one point...also expensive.  They have held up very well though.
> 
> 
> 
> Ed Gray | Pathology IT Analyst | Phone: 304-293-2945 | Fax 304-293-1627 | WVU Healthcare l egray <@t> wvuhealthcare.com
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> ------------------------------
> 
> Message: 3
> Date: Thu, 27 Jun 2013 19:23:06 +0000
> From: Nancy Schmitt <Nancy_Schmitt <@t> pa-ucl.com>
> Subject: [Histonet] Dragon users
> To: "Histonet (histonet <@t> lists.utsouthwestern.edu)"
>    <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
>    <906B4DA90ED1DB4DB6C7E94D7CEE6C36813D52E1 <@t> PEITHA.wad.pa-ucl.com>
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> 
> Hi All-
> 
> we are using Dragon Enterprise and currently using Plantronics wireless headsets - does anyone have any experience with other wireless microphone options?
> 
> Thanks for your input-
> 
> NancySchmitt
> 
> Dubuque, IA
> 
> 
> 
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> 
> 
> ------------------------------
> 
> Message: 4
> Date: Fri, 28 Jun 2013 16:25:05 +0000
> From: "Willis, Donna G." <Donna.Willis <@t> baylorhealth.edu>
> Subject: [Histonet] PowerPath Issues
> To: "histonet <@t> lists.utsouthwestern.edu"
>    <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
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> 
> Anyone out there in Histoland having issues with PowerPath (Sunquest) customer service?
> 
> Thanks,
> 
> Donna Willis, HT/HTL (ASCP)
> Anatomic Pathology Manager
> Baylor University Medical Center-Dallas
> ph. 214-820-2465 office
> ph. 214-725-6184 mobile
> donna.willis <@t> baylorhealth.edu
> 
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> 
> ------------------------------
> 
> Message: 5
> Date: Fri, 28 Jun 2013 19:01:19 +0000
> From: Jesus Ellin <JEllin <@t> yumaregional.org>
> Subject: Re: [Histonet] PowerPath Issues
> To: "Willis, Donna G." <Donna.Willis <@t> baylorhealth.edu>
> Cc: "histonet <@t> lists.utsouthwestern.edu"
>    <histonet <@t> lists.utsouthwestern.edu>,    Debbie Balli
>    <Debbie.Balli <@t> elekta.com>
> Message-ID: <A6D607C5-AAE0-4552-89CB-467EB8F3528C <@t> yumaregional.org>
> Content-Type: text/plain; charset="us-ascii"
> 
> Donna this is Jesus Ellin and I am the Sunquest POwerPath Chair,, I am cc Debbie Balli on this,, how can we help?
> 
> Sent from my iPad
> 
> On Jun 28, 2013, at 1:58 PM, "Willis, Donna G." <Donna.Willis <@t> baylorhealth.edu> wrote:
> 
>> Anyone out there in Histoland having issues with PowerPath (Sunquest) customer service?
>> 
>> Thanks,
>> 
>> Donna Willis, HT/HTL (ASCP)
>> Anatomic Pathology Manager
>> Baylor University Medical Center-Dallas
>> ph. 214-820-2465 office
>> ph. 214-725-6184 mobile
>> donna.willis <@t> baylorhealth.edu
>> 
>> **********************************************************************
>> This e-mail may contain confidential and/or privileged information. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information.
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> 
> ------------------------------
> 
> Message: 6
> Date: Fri, 28 Jun 2013 19:36:46 +0000
> From: CHRISTIE GOWAN <christiegowan <@t> msn.com>
> Subject: [Histonet] Alport Syndrome
> To: "histonet <@t> lists.utsouthwestern.edu"
>    <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <BLU173-W1098BFA29FEB1B10CDA647AE760 <@t> phx.gbl>
> Content-Type: text/plain; charset="iso-8859-1"
> 
> Is anyone using the Fluorchrome-Conjugated MoAbs for Alport Syndrome distributed by Cosmo Bio Co., LTD.?
> 
> We are using it on cryostat sections of renal tissue and the Texas Red fluorochrome seems to be extremely robust while the FITC-conjugated-anti alpha2(IV) is weak. I would be interested to hear your outcomes/solutions. 
> 
> 
> 
> Christie Gowan HT (ASCP)
> UAB Hospital Surgical Pathology
> Supervisor Lab Services
> 205-934-4991
> cgowan <@t> uabmc.edu
> 
>                         
> 
> ------------------------------
> 
> Message: 7
> Date: Fri, 28 Jun 2013 20:17:18 +0000
> From: "Nathan Ostlie (S)" <Nathan.Ostlie <@t> ColoradoCollege.edu>
> Subject: [Histonet] antibody providers for cytokine/chemokine IHC
> To: "histonet <@t> lists.utsouthwestern.edu"
>    <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
>    <8b449980f6f94ef684bd9dc45c3c7f44 <@t> BY2PR06MB012.namprd06.prod.outlook.com>
>    
> Content-Type: text/plain; charset="us-ascii"
> 
> Hello,
> 
> I am new to immunohistochemistry and I am in the process of ordering primary antibody for cytokine/chemokine staining. Does anyone have recommendations for companies that sell antibody compatible with IHC?
> 
> Examples of cytokines/chemokines we're hoping to stain for: CXCL5, CCL2, CXCL10, IL-6, IL-17 IFN-g
> 
> Thanks, Nate
> 
> 
> ------------------------------
> 
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> End of Histonet Digest, Vol 115, Issue 32
> *****************************************



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