[Histonet] anyone running PAX5 on smears

[Susan Foreman]

Hello, histonetters, this feed has proven helpful time after time.  Does
anyone run PAX5 (currently we are using DAKO antibody on Ventana stainer) on
smears?  We have it worked up in ffpe, but having some trouble getting
staining with the smears.   We have been acetone fixing the smears and the
cells have stayed on beautifully, but no staining of the Bcells.  I would
love the help this researcher out with beautiful slides really soon J.

 

 

Many Thanks, 

Susan Foreman, HT