From jshea121 <@t> roadrunner.com Sat Jun 1 18:25:37 2013 From: jshea121 <@t> roadrunner.com (Shea's) Date: Sat Jun 1 18:25:41 2013 Subject: [Histonet] bone marrow aspirations Message-ID: <38E00305B4744EDB9C25EE966E2030D9@JoannePC> I am also interested in how others are processing bone marrow aspirates. Currently, we let the aspirate clot on a watch glass for about 20 minutes, gentle slide it onto filter paper, roll it around to get rid of the excess fluid, place the clot between sponges, and process as usual. However, I am interested in knowing if any one out there is using a technique that we use to use at another hospital 33 years ago. If I remember correctly, the pathologist or oncologist would collect the aspirate using a heparin syringe. Some how, the aspirate smears for Hematology were made on 22x22 cover slips instead of slides. Then the EDTA tube was then given to Histology. I can't remember if we added formalin or B5 fixative to it at that time, but it was filtered into an obex tea bag type filter paper (The blood never clotted and ran through the filter paper, leaving only bone marrow spicules in the paper). We would fold the paper, place it in the cassette, and process as usual. The pathologist preferred this method because he wouldn't have to look at levels of clot to find spicules. Is there anyone out there using this method? Jan From rsrichmond <@t> gmail.com Sun Jun 2 12:23:13 2013 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Sun Jun 2 12:23:18 2013 Subject: [Histonet] Re: bone marrow aspirations Message-ID: At a time (about 35 years ago) when I was performing a lot of bone marrow aspirations and biopsies and had full control over my methods, I would perform a Westerman-Jensen needle biopsy, fixing that core specimen immediately and later decalcifying it, followed by a guardless Rosenthal needle aspiration (both in the posterior iliac crest). I would squirt some of the aspirate onto a slanted slide to pick out particles (don't call them spicules - that's bone) and prepare smears (or rather, have my assistant do that). I would then squirt some of the aspirate directly into neutral buffered formalin and give it a gentle shake. NBF doesn't clot blood, so I'd have a suspension of marrow particles I'd filter through a tea bag (now I'd use one of those little nylon bags) to be embedded as a cell block. I'd then let the rest of the aspirate clot in the syringe, so I'd also have a clot section block. I'd fix the core specimen and the clot in Zenker/Helly fixative, which of course you can't do today. I'd thus have the smears, and three paraffin blocks. My yield of metastatic cancer was probably a good bit higher than with less exacting methods. Bob Richmond Samurai Pathologist Maryville TN From mstone <@t> cmhlink.org Mon Jun 3 05:07:31 2013 From: mstone <@t> cmhlink.org (MARCELLYN A. STONE) Date: Mon Jun 3 05:07:39 2013 Subject: [Histonet] Pneumocystis bronch wash/sputum control slides Message-ID: <6FF1D287A4C72844B37CF1B0FBAAB05CD865811F@Exch-mbx-02.corp.cmhlink.org> I am looking for a supplier of pneumocystis positive control slides for sputum/bronch wash not tissue. Can anyone help me? Thanks Marcy CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for use by the designated recipients named above. They are intended solely for these recipients. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify Calvert Memorial Hospital immediately by telephone at (410)535-8282 and destroy all copies of this communication and any attachments. From asmith <@t> mail.barry.edu Mon Jun 3 08:09:12 2013 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Mon Jun 3 08:09:24 2013 Subject: [Histonet] Formalin In-Reply-To: <1370018477.61740.YahooMailNeo@web163103.mail.bf1.yahoo.com> References: <1370018477.61740.YahooMailNeo@web163103.mail.bf1.yahoo.com> Message-ID: <93D1C752AF965C43AE4C84D6D45CFC2FDFEF5B@BL2PRD0710MB373.namprd07.prod.outlook.com> Formalin does burn. If you soak a piece of paper in formalin, you can set it on fire with a match. Formalin is also slightly carcinogenic. The biggest problem with formalin is that a few people are severely allergic to it. -Allen A. Smith, Ph.D. Barry University School of Podiatric Medicine -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, May 31, 2013 12:41 PM To: Scott, Allison D; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Formalin Formalin is THEORETICALLY (and very UNLIKELY) "flammable, but putting it into a flammables cabinet is going too far. Try to find out those conflicting standards (JCAHO vs. CAP) and follow the strictest. Also please remember that many inspectors just "love to show off!" Some think that have to "cite for something" because otherwise they are not doing a good job, or do not know enough. Ren? J. From: "Scott, Allison D" To: "histonet@lists.utsouthwestern.edu" Sent: Friday, May 31, 2013 12:31 PM Subject: [Histonet] Formalin Hello to all in histoland.? Happy Friday.? Last week we were inspected by a new group of surveyors that are like JCAHO called DNV.? They said that we had to much formalin stored in our gross room.? We had 15 gallons.? They told us that we could only have 2 gallons there at a time.? I have never heard of such.? We were just inspected by CAP and I might add that it was an? intense inspection.? They checked everything. This never came up.? What is the recommended amount of formalin that can be stored in an area.? Does it need to be in a flammable cabinet?? The cabinet that we have the formalin in is so old that we are? having a vendor to come out to check to see if it suitable for chemical storage. Allison Scott HT(ASCP) Supervisor, Histology Lab LBJ Hospital Harris Health System Office: 713-566-2148 Lab: 713-566-5287 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged.? This e-mail may also be confidential and/or privileged under Texas law.? The e-mail is for the use of only the individual or entity named above.? If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tgenade <@t> gmail.com Mon Jun 3 13:30:08 2013 From: tgenade <@t> gmail.com (Tyrone Genade) Date: Mon Jun 3 13:30:14 2013 Subject: [Histonet] picric acid paranoia Message-ID: Hello, I am moving to the USA from sunny South Africa. I would like to bring my wax blocks with me but the fish inside them were fixed with Bouin's fluid. I'm worried the picric acid could draw the wrong sort of attention. Courier companies and US Customs (which never got back to me) haven't been able to give me an answer if they are safe to travel. The blocks have sat under my lab bench for 4 years without blowing up so I guess they are perfectly safe. Anyone have an opinion on the issues or some advice on an expert (at US customs?) to contact? I would probably ship them by surface post as it just more cost effective. Thanks Tyrone Genade PhD Department of Human Biology University of Cape Town From llewllew <@t> shaw.ca Mon Jun 3 14:04:56 2013 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Mon Jun 3 14:05:14 2013 Subject: [Histonet] picric acid paranoia In-Reply-To: References: Message-ID: <51ACE8D8.1080305@shaw.ca> I don't know what the US regulations for importing paraffin blocks are, but Bouin fixed and paraffin processed tissues are perfectly safe. It is picric acid and its simple salts which are dangerous when dried. Since your tissues went through alcohol during processing, any free picric acid will have been removed and only that attached to the proteins remains. In fact, the paraffin wax itself is likely more of a hazard since it is inflammable. I suspect they could be safely shipped by mail, with appropriate declarations. Bryan Llewellyn Tyrone Genade wrote: > Hello, > > I am moving to the USA from sunny South Africa. I would like to bring my > wax blocks with me but the fish inside them were fixed with Bouin's fluid. > I'm worried the picric acid could draw the wrong sort of attention. Courier > companies and US Customs (which never got back to me) haven't been able to > give me an answer if they are safe to travel. The blocks have sat under my > lab bench for 4 years without blowing up so I guess they are perfectly > safe. Anyone have an opinion on the issues or some advice on an expert (at > US customs?) to contact? I would probably ship them by surface post as it > just more cost effective. > > Thanks > > Tyrone Genade PhD > Department of Human Biology > University of Cape Town > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Timothy.Morken <@t> ucsfmedctr.org Mon Jun 3 14:10:14 2013 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Mon Jun 3 14:12:22 2013 Subject: [Histonet] picric acid paranoia In-Reply-To: <51ACE8D8.1080305@shaw.ca> References: <51ACE8D8.1080305@shaw.ca> Message-ID: <761E2B5697F795489C8710BCC72141FF0826F5@ex07.net.ucsf.edu> I agree with Bryan, the only dangerous form is anhydrous powder. I'm thinking they might be more interested in having you declare these blocks are not infectious... Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bryan Llewellyn Sent: Monday, June 03, 2013 12:05 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] picric acid paranoia I don't know what the US regulations for importing paraffin blocks are, but Bouin fixed and paraffin processed tissues are perfectly safe. It is picric acid and its simple salts which are dangerous when dried. Since your tissues went through alcohol during processing, any free picric acid will have been removed and only that attached to the proteins remains. In fact, the paraffin wax itself is likely more of a hazard since it is inflammable. I suspect they could be safely shipped by mail, with appropriate declarations. Bryan Llewellyn Tyrone Genade wrote: > Hello, > > I am moving to the USA from sunny South Africa. I would like to bring > my wax blocks with me but the fish inside them were fixed with Bouin's fluid. > I'm worried the picric acid could draw the wrong sort of attention. > Courier companies and US Customs (which never got back to me) haven't > been able to give me an answer if they are safe to travel. The blocks > have sat under my lab bench for 4 years without blowing up so I guess > they are perfectly safe. Anyone have an opinion on the issues or some > advice on an expert (at US customs?) to contact? I would probably ship > them by surface post as it just more cost effective. > > Thanks > > Tyrone Genade PhD > Department of Human Biology > University of Cape Town > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kvickers <@t> westmoreland-derm.com Mon Jun 3 14:18:08 2013 From: kvickers <@t> westmoreland-derm.com (Karen Vickers) Date: Mon Jun 3 14:18:56 2013 Subject: [Histonet] Job Opening Message-ID: <004901ce608f$16026a90$42073fb0$@com> We have a job opening in our dermatopathology lab for a Histotechnician. If interested, please submit resume to: Westmoreland Dermatology P. O. Box 8695 Columbus, MS 39705 or email kvickers@westmoreland-derm.com Thank you, Karen Vickers Westmoreland Dermatology Administrator phone 662-243-2435 fax 662-328-7037 cell 662-425-3769 From shive003 <@t> umn.edu Mon Jun 3 15:30:04 2013 From: shive003 <@t> umn.edu (Jan Shivers) Date: Mon Jun 3 15:30:07 2013 Subject: [Histonet] Highlands J Virus Message-ID: (Asking for a colleague...) Is there any lab out there currently doing IHC for Highlands J Virus? You can message me privately. Thanks in advance, Jan Shivers Senior Scientist Univ. of Minnesota Veterinary Diagnostic Lab shive003@umn.edu From asmith <@t> mail.barry.edu Tue Jun 4 07:52:26 2013 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Tue Jun 4 07:52:38 2013 Subject: [Histonet] picric acid paranoia In-Reply-To: References: Message-ID: <93D1C752AF965C43AE4C84D6D45CFC2FDFF156@BL2PRD0710MB373.namprd07.prod.outlook.com> Picric acid bound to collagen is not an explosion hazard. Even if it were, the surrounding paraffin wax would cushion the picric acid to the point of making it shockproof. Most of the picric acid in a fixative ends up in the hazmat bottle rather than in the tissue. Thus even putting 50 or so blocks of tissue fixed in picric acid into a hot fire would create less blast than a hearing aid battery. Bulk picric acid, where there is no moderator between the crystals, is another story. - Allen A. Smith, Ph.D. Barry University School of Podiatric Medicine -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tyrone Genade Sent: Monday, June 03, 2013 2:30 PM To: histonet Subject: [Histonet] picric acid paranoia Hello, I am moving to the USA from sunny South Africa. I would like to bring my wax blocks with me but the fish inside them were fixed with Bouin's fluid. I'm worried the picric acid could draw the wrong sort of attention. Courier companies and US Customs (which never got back to me) haven't been able to give me an answer if they are safe to travel. The blocks have sat under my lab bench for 4 years without blowing up so I guess they are perfectly safe. Anyone have an opinion on the issues or some advice on an expert (at US customs?) to contact? I would probably ship them by surface post as it just more cost effective. Thanks Tyrone Genade PhD Department of Human Biology University of Cape Town _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From chapcl <@t> yahoo.com Tue Jun 4 08:22:04 2013 From: chapcl <@t> yahoo.com (Will Chappell) Date: Tue Jun 4 08:22:12 2013 Subject: [Histonet] picric acid paranoia In-Reply-To: <93D1C752AF965C43AE4C84D6D45CFC2FDFF156@BL2PRD0710MB373.namprd07.prod.outlook.com> References: <93D1C752AF965C43AE4C84D6D45CFC2FDFF156@BL2PRD0710MB373.namprd07.prod.outlook.com> Message-ID: <57AFF7C7-473B-4820-A3F4-6BDF70AEBD79@yahoo.com> Ship them as you would any biological test samples. No problems here. Sent from my iPhone On Jun 4, 2013, at 5:52 AM, "Smith, Allen" wrote: > Picric acid bound to collagen is not an explosion hazard. Even if it were, the surrounding paraffin wax would cushion the picric acid to the point of making it shockproof. Most of the picric acid in a fixative ends up in the hazmat bottle rather than in the tissue. Thus even putting 50 or so blocks of tissue fixed in picric acid into a hot fire would create less blast than a hearing aid battery. > Bulk picric acid, where there is no moderator between the crystals, is another story. > - Allen A. Smith, Ph.D. > Barry University School of Podiatric Medicine > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tyrone Genade > Sent: Monday, June 03, 2013 2:30 PM > To: histonet > Subject: [Histonet] picric acid paranoia > > Hello, > > I am moving to the USA from sunny South Africa. I would like to bring my wax blocks with me but the fish inside them were fixed with Bouin's fluid. > I'm worried the picric acid could draw the wrong sort of attention. Courier companies and US Customs (which never got back to me) haven't been able to give me an answer if they are safe to travel. The blocks have sat under my lab bench for 4 years without blowing up so I guess they are perfectly safe. Anyone have an opinion on the issues or some advice on an expert (at US customs?) to contact? I would probably ship them by surface post as it just more cost effective. > > Thanks > > Tyrone Genade PhD > Department of Human Biology > University of Cape Town > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From christina.thurby <@t> bms.com Tue Jun 4 13:21:50 2013 From: christina.thurby <@t> bms.com (Thurby, Christina) Date: Tue Jun 4 13:22:06 2013 Subject: [Histonet] Anyone know if commercial antibodies to KCA-1 and/or KCA-2 (Kupffer cell antibodies) are available? Message-ID: Hi all, I have been searching for a Kupffer cell antibody. I read a paper that KCA-1 and KCA-2 are two monoclonal antibodies that are specific for Kupffer cells (PMCID: PMC1877398) . I am looking for a vendor that may have these antibodies available commercially. Alternatively, if anyone knows of other antibodies that are specific for Kupffer cells that would also be helpful! Thanks! Christina Thurby Bristol Myers Squibb 812-307-2093 ________________________________ This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. From liz <@t> premierlab.com Tue Jun 4 14:03:23 2013 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Tue Jun 4 14:03:38 2013 Subject: [Histonet] Anyone know if commercial antibodies to KCA-1 and/or KCA-2 (Kupffer cell antibodies) are available? In-Reply-To: References: Message-ID: <14E2C6176416974295479C64A11CB9AE016411AC2F55@SBS2K8.premierlab.local> Christy This is not necessarily specific for kupffer cells it will stain other macrophages but F4/80 does stain Kupffer cells. I guess I should ask which species, ED-1 will work in rat, the F4/80 in mouse and I have heard that Iba-1 stains macrophages in mice nicely that's a microglia marker, but we have not tried it on any other tissue than brain so far. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Laboratory Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 Work (303) 682-3949 Fax (303) 682-9060 Cell (303) 881-0763 liz@premierlab.com www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Thurby, Christina Sent: Tuesday, June 04, 2013 12:22 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Anyone know if commercial antibodies to KCA-1 and/or KCA-2 (Kupffer cell antibodies) are available? Hi all, I have been searching for a Kupffer cell antibody. I read a paper that KCA-1 and KCA-2 are two monoclonal antibodies that are specific for Kupffer cells (PMCID: PMC1877398) . I am looking for a vendor that may have these antibodies available commercially. Alternatively, if anyone knows of other antibodies that are specific for Kupffer cells that would also be helpful! Thanks! Christina Thurby Bristol Myers Squibb 812-307-2093 ________________________________ This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. From JWatson <@t> gnf.org Tue Jun 4 14:08:50 2013 From: JWatson <@t> gnf.org (James Watson) Date: Tue Jun 4 14:08:55 2013 Subject: [Histonet] Anyone know if commercial antibodies to KCA-1 and/or KCA-2 (Kupffer cell antibodies) are available? In-Reply-To: <14E2C6176416974295479C64A11CB9AE016411AC2F55@SBS2K8.premierlab.local> References: <14E2C6176416974295479C64A11CB9AE016411AC2F55@SBS2K8.premierlab.local> Message-ID: We are in the process of working up the Iba1 on mouse and it does stain the Kupffer cells, macrophages, and microglial James Watson HT? ASCP GNF? Genomics Institute of the Novartis Research Foundation Tel??? 858-332-4647 Fax?? 858-812-1915 jwatson@gnf.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala Sent: Tuesday, June 04, 2013 12:03 PM To: Thurby, Christina; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Anyone know if commercial antibodies to KCA-1 and/or KCA-2 (Kupffer cell antibodies) are available? Christy This is not necessarily specific for kupffer cells it will stain other macrophages but F4/80 does stain Kupffer cells. I guess I should ask which species, ED-1 will work in rat, the F4/80 in mouse and I have heard that Iba-1 stains macrophages in mice nicely that's a microglia marker, but we have not tried it on any other tissue than brain so far. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Laboratory Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 Work (303) 682-3949 Fax (303) 682-9060 Cell (303) 881-0763 liz@premierlab.com www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Thurby, Christina Sent: Tuesday, June 04, 2013 12:22 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Anyone know if commercial antibodies to KCA-1 and/or KCA-2 (Kupffer cell antibodies) are available? Hi all, I have been searching for a Kupffer cell antibody. I read a paper that KCA-1 and KCA-2 are two monoclonal antibodies that are specific for Kupffer cells (PMCID: PMC1877398) . I am looking for a vendor that may have these antibodies available commercially. Alternatively, if anyone knows of other antibodies that are specific for Kupffer cells that would also be helpful! Thanks! Christina Thurby Bristol Myers Squibb 812-307-2093 ________________________________ This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From christina.thurby <@t> bms.com Tue Jun 4 14:19:16 2013 From: christina.thurby <@t> bms.com (Thurby, Christina) Date: Tue Jun 4 14:19:26 2013 Subject: [Histonet] Anyone know if commercial antibodies to KCA-1 and/or KCA-2 (Kupffer cell antibodies) are available? In-Reply-To: References: <14E2C6176416974295479C64A11CB9AE016411AC2F55@SBS2K8.premierlab.local> Message-ID: Thanks James and Liz! I'll look into the Iba1, this is an antibody I have not looked at yet! Christina >-----Original Message----- >From: James Watson [mailto:JWatson@gnf.org] >Sent: Tuesday, June 04, 2013 2:09 PM >To: 'Elizabeth Chlipala'; Thurby, Christina; >histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] Anyone know if commercial antibodies to KCA-1 >and/or KCA-2 (Kupffer cell antibodies) are available? > >We are in the process of working up the Iba1 on mouse and it does stain >the Kupffer cells, macrophages, and microglial > >James Watson HT ASCP >GNF Genomics Institute of the Novartis Research Foundation >Tel 858-332-4647 >Fax 858-812-1915 >jwatson@gnf.org > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet- >bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala >Sent: Tuesday, June 04, 2013 12:03 PM >To: Thurby, Christina; histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] Anyone know if commercial antibodies to KCA-1 >and/or KCA-2 (Kupffer cell antibodies) are available? > >Christy > >This is not necessarily specific for kupffer cells it will stain other >macrophages but F4/80 does stain Kupffer cells. I guess I should ask >which species, ED-1 will work in rat, the F4/80 in mouse and I have >heard that Iba-1 stains macrophages in mice nicely that's a microglia >marker, but we have not tried it on any other tissue than brain so far. > >Liz > >Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Laboratory Manager Premier >Laboratory, LLC PO Box 18592 Boulder, CO 80308 Work (303) 682-3949 Fax >(303) 682-9060 Cell (303) 881-0763 liz@premierlab.com www.premierlab.com > >Ship to address: > >1567 Skyway Drive, Unit E >Longmont, CO 80504 > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet- >bounces@lists.utsouthwestern.edu] On Behalf Of Thurby, Christina >Sent: Tuesday, June 04, 2013 12:22 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Anyone know if commercial antibodies to KCA-1 and/or >KCA-2 (Kupffer cell antibodies) are available? > >Hi all, >I have been searching for a Kupffer cell antibody. I read a paper that >KCA-1 and KCA-2 are two monoclonal antibodies that are specific for >Kupffer cells (PMCID: PMC1877398) . I am looking for a vendor that may >have these antibodies available commercially. Alternatively, if anyone >knows of other antibodies that are specific for Kupffer cells that would >also be helpful! >Thanks! > >Christina Thurby >Bristol Myers Squibb >812-307-2093 > > > > >________________________________ >This message (including any attachments) may contain confidential, >proprietary, privileged and/or private information. The information is >intended to be for the use of the individual or entity designated above. >If you are not the intended recipient of this message, please notify the >sender immediately, and delete the message and any attachments. Any >disclosure, reproduction, distribution or other use of this message or >any attachments by an individual or entity other than the intended >recipient is prohibited. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. From Rcartun <@t> harthosp.org Tue Jun 4 15:28:59 2013 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Tue Jun 4 15:29:18 2013 Subject: [Histonet] Question - MDM Message-ID: <51AE15CB020000770003B61B@gwmail3.harthosp.org> Is anyone using a commercially available antibody (not RUO) for the immunohistochemical detection of MDM in liposarcoma? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax From Andrea.X.Dejager <@t> kp.org Tue Jun 4 15:31:15 2013 From: Andrea.X.Dejager <@t> kp.org (Andrea.X.Dejager@kp.org) Date: Tue Jun 4 15:31:34 2013 Subject: [Histonet] picric acid In-Reply-To: <201306041754.r54Hs4ij017613@pps.reinject> References: <201306041754.r54Hs4ij017613@pps.reinject> Message-ID: Considering tissues were processed and are in paraffin there is no need to panic, Picric acid was removed by solutions on the tissue processor. Andrea De Jager, H.T. ASCP Histology Manager, Regional Reference Lab Kaiser Permanente - Colorado Phone: 303-404-4152 Fax: 303-404-4161 email: ANDREA.X.DEJAGER@KP.org NOTICE TO RECIPIENT: If you are not the intended recipient of this e-mail, you are prohibited from sharing, copying, or otherwise using or disclosing its contents. If you have received this e-mail in error, please notify the sender immediately by reply e-mail and permanently delete this e-mail and any attachments without reading, forwarding or saving them. Thank you. From: histonet-request@lists.utsouthwestern.edu To: histonet@lists.utsouthwestern.edu Date: 06/04/2013 12:02 PM Subject: Histonet Digest, Vol 115, Issue 4 Sent by: histonet-bounces@lists.utsouthwestern.edu Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. picric acid paranoia (Tyrone Genade) 2. Re: picric acid paranoia (Bryan Llewellyn) 3. RE: picric acid paranoia (Morken, Timothy) 4. Job Opening (Karen Vickers) 5. Highlands J Virus (Jan Shivers) 6. RE: picric acid paranoia (Smith, Allen) 7. Re: picric acid paranoia (Will Chappell) ---------------------------------------------------------------------- Message: 1 Date: Mon, 3 Jun 2013 20:30:08 +0200 From: Tyrone Genade Subject: [Histonet] picric acid paranoia To: histonet Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hello, I am moving to the USA from sunny South Africa. I would like to bring my wax blocks with me but the fish inside them were fixed with Bouin's fluid. I'm worried the picric acid could draw the wrong sort of attention. Courier companies and US Customs (which never got back to me) haven't been able to give me an answer if they are safe to travel. The blocks have sat under my lab bench for 4 years without blowing up so I guess they are perfectly safe. Anyone have an opinion on the issues or some advice on an expert (at US customs?) to contact? I would probably ship them by surface post as it just more cost effective. Thanks Tyrone Genade PhD Department of Human Biology University of Cape Town ------------------------------ Message: 2 Date: Mon, 03 Jun 2013 12:04:56 -0700 From: Bryan Llewellyn Subject: Re: [Histonet] picric acid paranoia To: histonet@lists.utsouthwestern.edu Message-ID: <51ACE8D8.1080305@shaw.ca> Content-Type: text/plain; charset=ISO-8859-1; format=flowed I don't know what the US regulations for importing paraffin blocks are, but Bouin fixed and paraffin processed tissues are perfectly safe. It is picric acid and its simple salts which are dangerous when dried. Since your tissues went through alcohol during processing, any free picric acid will have been removed and only that attached to the proteins remains. In fact, the paraffin wax itself is likely more of a hazard since it is inflammable. I suspect they could be safely shipped by mail, with appropriate declarations. Bryan Llewellyn Tyrone Genade wrote: > Hello, > > I am moving to the USA from sunny South Africa. I would like to bring my > wax blocks with me but the fish inside them were fixed with Bouin's fluid. > I'm worried the picric acid could draw the wrong sort of attention. Courier > companies and US Customs (which never got back to me) haven't been able to > give me an answer if they are safe to travel. The blocks have sat under my > lab bench for 4 years without blowing up so I guess they are perfectly > safe. Anyone have an opinion on the issues or some advice on an expert (at > US customs?) to contact? I would probably ship them by surface post as it > just more cost effective. > > Thanks > > Tyrone Genade PhD > Department of Human Biology > University of Cape Town > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 3 Date: Mon, 3 Jun 2013 19:10:14 +0000 From: "Morken, Timothy" Subject: RE: [Histonet] picric acid paranoia To: "histonet@lists.utsouthwestern.edu" Message-ID: <761E2B5697F795489C8710BCC72141FF0826F5@ex07.net.ucsf.edu> Content-Type: text/plain; charset=us-ascii I agree with Bryan, the only dangerous form is anhydrous powder. I'm thinking they might be more interested in having you declare these blocks are not infectious... Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [ mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bryan Llewellyn Sent: Monday, June 03, 2013 12:05 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] picric acid paranoia I don't know what the US regulations for importing paraffin blocks are, but Bouin fixed and paraffin processed tissues are perfectly safe. It is picric acid and its simple salts which are dangerous when dried. Since your tissues went through alcohol during processing, any free picric acid will have been removed and only that attached to the proteins remains. In fact, the paraffin wax itself is likely more of a hazard since it is inflammable. I suspect they could be safely shipped by mail, with appropriate declarations. Bryan Llewellyn Tyrone Genade wrote: > Hello, > > I am moving to the USA from sunny South Africa. I would like to bring > my wax blocks with me but the fish inside them were fixed with Bouin's fluid. > I'm worried the picric acid could draw the wrong sort of attention. > Courier companies and US Customs (which never got back to me) haven't > been able to give me an answer if they are safe to travel. The blocks > have sat under my lab bench for 4 years without blowing up so I guess > they are perfectly safe. Anyone have an opinion on the issues or some > advice on an expert (at US customs?) to contact? I would probably ship > them by surface post as it just more cost effective. > > Thanks > > Tyrone Genade PhD > Department of Human Biology > University of Cape Town > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Mon, 3 Jun 2013 14:18:08 -0500 From: "Karen Vickers" Subject: [Histonet] Job Opening To: Message-ID: <004901ce608f$16026a90$42073fb0$@com> Content-Type: text/plain; charset="us-ascii" We have a job opening in our dermatopathology lab for a Histotechnician. If interested, please submit resume to: Westmoreland Dermatology P. O. Box 8695 Columbus, MS 39705 or email kvickers@westmoreland-derm.com Thank you, Karen Vickers Westmoreland Dermatology Administrator phone 662-243-2435 fax 662-328-7037 cell 662-425-3769 ------------------------------ Message: 5 Date: Mon, 3 Jun 2013 15:30:04 -0500 From: Jan Shivers Subject: [Histonet] Highlands J Virus To: histonet Message-ID: Content-Type: text/plain; charset=ISO-8859-1 (Asking for a colleague...) Is there any lab out there currently doing IHC for Highlands J Virus? You can message me privately. Thanks in advance, Jan Shivers Senior Scientist Univ. of Minnesota Veterinary Diagnostic Lab shive003@umn.edu ------------------------------ Message: 6 Date: Tue, 4 Jun 2013 12:52:26 +0000 From: "Smith, Allen" Subject: RE: [Histonet] picric acid paranoia To: "tgenade@gmail.com" Cc: "histonet@lists.utsouthwestern.edu" Message-ID: <93D1C752AF965C43AE4C84D6D45CFC2FDFF156@BL2PRD0710MB373.namprd07.prod.outlook.com> Content-Type: text/plain; charset="us-ascii" Picric acid bound to collagen is not an explosion hazard. Even if it were, the surrounding paraffin wax would cushion the picric acid to the point of making it shockproof. Most of the picric acid in a fixative ends up in the hazmat bottle rather than in the tissue. Thus even putting 50 or so blocks of tissue fixed in picric acid into a hot fire would create less blast than a hearing aid battery. Bulk picric acid, where there is no moderator between the crystals, is another story. - Allen A. Smith, Ph.D. Barry University School of Podiatric Medicine -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [ mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tyrone Genade Sent: Monday, June 03, 2013 2:30 PM To: histonet Subject: [Histonet] picric acid paranoia Hello, I am moving to the USA from sunny South Africa. I would like to bring my wax blocks with me but the fish inside them were fixed with Bouin's fluid. I'm worried the picric acid could draw the wrong sort of attention. Courier companies and US Customs (which never got back to me) haven't been able to give me an answer if they are safe to travel. The blocks have sat under my lab bench for 4 years without blowing up so I guess they are perfectly safe. Anyone have an opinion on the issues or some advice on an expert (at US customs?) to contact? I would probably ship them by surface post as it just more cost effective. Thanks Tyrone Genade PhD Department of Human Biology University of Cape Town _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Tue, 4 Jun 2013 06:22:04 -0700 From: Will Chappell Subject: Re: [Histonet] picric acid paranoia To: "Smith, Allen" Cc: "histonet@lists.utsouthwestern.edu" , "tgenade@gmail.com" Message-ID: <57AFF7C7-473B-4820-A3F4-6BDF70AEBD79@yahoo.com> Content-Type: text/plain; charset=us-ascii Ship them as you would any biological test samples. No problems here. Sent from my iPhone On Jun 4, 2013, at 5:52 AM, "Smith, Allen" wrote: > Picric acid bound to collagen is not an explosion hazard. Even if it were, the surrounding paraffin wax would cushion the picric acid to the point of making it shockproof. Most of the picric acid in a fixative ends up in the hazmat bottle rather than in the tissue. Thus even putting 50 or so blocks of tissue fixed in picric acid into a hot fire would create less blast than a hearing aid battery. > Bulk picric acid, where there is no moderator between the crystals, is another story. > - Allen A. Smith, Ph.D. > Barry University School of Podiatric Medicine > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [ mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tyrone Genade > Sent: Monday, June 03, 2013 2:30 PM > To: histonet > Subject: [Histonet] picric acid paranoia > > Hello, > > I am moving to the USA from sunny South Africa. I would like to bring my wax blocks with me but the fish inside them were fixed with Bouin's fluid. > I'm worried the picric acid could draw the wrong sort of attention. Courier companies and US Customs (which never got back to me) haven't been able to give me an answer if they are safe to travel. The blocks have sat under my lab bench for 4 years without blowing up so I guess they are perfectly safe. Anyone have an opinion on the issues or some advice on an expert (at US customs?) to contact? I would probably ship them by surface post as it just more cost effective. > > Thanks > > Tyrone Genade PhD > Department of Human Biology > University of Cape Town > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 115, Issue 4 **************************************** From CObregon <@t> mhs.net Tue Jun 4 16:34:02 2013 From: CObregon <@t> mhs.net (Obregon, Cecilia) Date: Tue Jun 4 16:34:08 2013 Subject: [Histonet] PAX-8 background staining Ultra platform Message-ID: <598BB4A3A92D7F4DAA14C30E4AE70C2F10B1386E@MHSEXMB05.mhs.net> Anyone running PAX-8 on the Benchmark Ultra succesfully? I have tried numerous protocols and antibodies, and still not able to obtain good results. I'm getting some background/cytoplasmic staining especially in normal kidney along with nuclear staining in the distal tubules and collecting ducts. I can't seem to completely be able to get it of it without compromising the signal. I have tried the Cell Marque MRQ-50 clone, the Biocare BC12, and now trying the Polyclonal Proteintech ...which looks promising. It's hard to duplicate the protocols that other institutions have in place mostly because the platforms are different. Our institution only has Ventana platform, and they're suggesting we try their new detection kit for this marker: Optiview. I can't honestly imagine buying a detection for just one antibody... We currently run UltraView Detection. Any inputs or suggestions would be greatly appreciated. Cecilia M. Obregon Memorial Regional Hospital 3501 Johnson Street Hollywood, FL 33021 Ph: 954-265-5317 From b427297 <@t> aol.com Tue Jun 4 21:12:16 2013 From: b427297 <@t> aol.com (Jackie O'Connor) Date: Tue Jun 4 21:12:19 2013 Subject: [Histonet] 10% NBF classified as non-haz waste In-Reply-To: References: <201306041754.r54Hs4ij017613@pps.reinject> Message-ID: <8D02FA93B869E05-E20-4E1BB@webmail-d127.sysops.aol.com> Did I miss something? My division safety group has instructed me to consider used 10% NBF as non-hazardous waste. Do I need to get out more, or is it still toxic and a potential carcinogen? Can I add it to beer again to get that nice foamy head like in "Good Morning Vietnam"? After all, it's non-hazardous. From joel.haas <@t> gladstone.ucsf.edu Wed Jun 5 00:29:55 2013 From: joel.haas <@t> gladstone.ucsf.edu (Joel Haas) Date: Wed Jun 5 00:30:05 2013 Subject: [Histonet] Electron Microscopy protocols Message-ID: Hello all, I am working on developing an electron microscopy protocol for looking at lipid droplets in cultured cells. We have used thin section TEM previously and found that the morphology of the droplets is often deformed (should be spherical, shows up as egg shaped or lumpy). If possible we would like to avoid these types of preservation artifacts. Does anyone have suggestions for adapting the protocol to better preserve these types of structures--a triglyceride lipid core surrounded by a phospholipid monolayer? Thanks in advance, Joel From Jonathan.Cremer <@t> med.kuleuven.be Wed Jun 5 01:07:40 2013 From: Jonathan.Cremer <@t> med.kuleuven.be (Jonathan Cremer) Date: Wed Jun 5 01:07:49 2013 Subject: [Histonet] 10% NBF classified as non-haz waste In-Reply-To: <8D02FA93B869E05-E20-4E1BB@webmail-d127.sysops.aol.com> References: <201306041754.r54Hs4ij017613@pps.reinject> , <8D02FA93B869E05-E20-4E1BB@webmail-d127.sysops.aol.com> Message-ID: Tell your division safety group to stick their nose in a 10% NBF waste bottle and take a few deep breaths. Make sure you get it on video, so we can have a laugh. --- Jonathan Cremer Laboratory Technician TARGID - KU Leuven Gasthuisberg CDG; Labo Experimentele Immunologie; Herestraat 49 bus 811; 3000 Leuven; Belgium ________________________________________ Van: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] namens Jackie O'Connor [b427297@aol.com] Verzonden: woensdag 5 juni 2013 4:12 To: histonet@lists.utsouthwestern.edu Onderwerp: [Histonet] 10% NBF classified as non-haz waste Did I miss something? My division safety group has instructed me to consider used 10% NBF as non-hazardous waste. Do I need to get out more, or is it still toxic and a potential carcinogen? Can I add it to beer again to get that nice foamy head like in "Good Morning Vietnam"? After all, it's non-hazardous. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Stacy_McLaughlin <@t> cooley-dickinson.org Wed Jun 5 06:41:03 2013 From: Stacy_McLaughlin <@t> cooley-dickinson.org (Stacy McLaughlin) Date: Wed Jun 5 06:41:10 2013 Subject: [Histonet] 10% NBF classified as non-haz waste In-Reply-To: References: <201306041754.r54Hs4ij017613@pps.reinject> , <8D02FA93B869E05-E20-4E1BB@webmail-d127.sysops.aol.com> Message-ID: As crazy as this sounds, it is not considered a hazardous waste by USDOT standards. I don't agree with it. Chug a lug! Stacy McLaughlin, HT(ASCP) Histology Supervisor Cooley Dickinson Hospital 30 Locust Street Northampton, MA 01060 (413)582-2019 Stacy_McLaughlin@Cooley-Dickinson.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jonathan Cremer Sent: Wednesday, June 05, 2013 2:08 AM To: Jackie O'Connor; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] 10% NBF classified as non-haz waste Tell your division safety group to stick their nose in a 10% NBF waste bottle and take a few deep breaths. Make sure you get it on video, so we can have a laugh. --- Jonathan Cremer Laboratory Technician TARGID - KU Leuven Gasthuisberg CDG; Labo Experimentele Immunologie; Herestraat 49 bus 811; 3000 Leuven; Belgium ________________________________________ Van: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] namens Jackie O'Connor [b427297@aol.com] Verzonden: woensdag 5 juni 2013 4:12 To: histonet@lists.utsouthwestern.edu Onderwerp: [Histonet] 10% NBF classified as non-haz waste Did I miss something? My division safety group has instructed me to consider used 10% NBF as non-hazardous waste. Do I need to get out more, or is it still toxic and a potential carcinogen? Can I add it to beer again to get that nice foamy head like in "Good Morning Vietnam"? After all, it's non-hazardous. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Jun 5 07:59:37 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jun 5 07:59:42 2013 Subject: [Histonet] Electron Microscopy protocols In-Reply-To: References: Message-ID: <1370437177.52565.YahooMailNeo@web163104.mail.bf1.yahoo.com> Compressed fat cell cytoplasms (that is what you are seeing as deformed) are cause by to harsh dehydration. Dehydration for TEM specimens has to be extremely graded, without passing from one alcohol to the next with more that 5% gradient. Additionally fixation has to be complete. Everything in specimens for TEM has to be very gradual, something not very difficult to do in specimens that, as a norm, are 2mm cubes. Check your processing protocol. Ren? J. From: Joel Haas To: histonet@lists.utsouthwestern.edu Sent: Wednesday, June 5, 2013 1:29 AM Subject: [Histonet] Electron Microscopy protocols Hello all, ? ? I am working on developing an electron microscopy protocol for looking at lipid droplets in cultured cells. We have used thin section TEM previously and found that the morphology of the droplets is often deformed (should be spherical, shows up as egg shaped or lumpy). If possible we would like to avoid these types of preservation artifacts. Does anyone have suggestions for adapting the protocol to better preserve these types of structures--a triglyceride lipid core surrounded by a phospholipid monolayer? Thanks in advance, Joel _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Jun 5 08:03:20 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jun 5 08:03:24 2013 Subject: [Histonet] 10% NBF classified as non-haz waste In-Reply-To: <8D02FA93B869E05-E20-4E1BB@webmail-d127.sysops.aol.com> References: <201306041754.r54Hs4ij017613@pps.reinject> <8D02FA93B869E05-E20-4E1BB@webmail-d127.sysops.aol.com> Message-ID: <1370437400.55387.YahooMailNeo@web163104.mail.bf1.yahoo.com> Yes,?you missed something. 10% NBF is hazardous and carcinogenic and, by the way, do not add it to your beer because it is also poisonous, affects the brain causing heart and respiratory failures. Ren? J.? From: Jackie O'Connor To: histonet@lists.utsouthwestern.edu Sent: Tuesday, June 4, 2013 10:12 PM Subject: [Histonet] 10% NBF classified as non-haz waste Did I miss something?? My division safety group has instructed me to consider used 10% NBF as non-hazardous waste. Do I need to get out more, or is it still toxic and a potential carcinogen?? Can I add it to beer again to get that nice foamy head like in "Good Morning Vietnam"? After all, it's non-hazardous. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Liz.Drinkall <@t> nmhs.org Wed Jun 5 08:21:43 2013 From: Liz.Drinkall <@t> nmhs.org (Drinkall, Liz) Date: Wed Jun 5 08:24:37 2013 Subject: [Histonet] FISH controls Message-ID: Hi everyone, I am working in a brand new cytogenetics lab, and we have some questions about controls for Her2. Does your lab use a control for each run, and if so, what does your control contain? Have you ever used a Her2 positive cell line as a control? Finally, during our validations for FISH, is it enough to get the same result as Clarient or do we need to verify with another method? Thanks in advance for any help you can give. Liz Liz Drinkall, HTL (ASCP) CM Methodist Hospital Histology Lab/Nebraska Collaborative Lab 8303 Dodge St. Omaha, NE 68114 (402)354-4572/(402)354-7974 This message and any included attachments are from Nebraska Methodist Health System and its affiliates and are intended only for the addressee. The message may contain privileged, confidential and/or proprietary information intended only for the person(s) named. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Nebraska Methodist Health System and its affiliates in Omaha, Nebraska, U.S.A at (402)354-2280. From vperez <@t> pathreflab.com Wed Jun 5 08:46:30 2013 From: vperez <@t> pathreflab.com (Vanessa Perez) Date: Wed Jun 5 08:46:36 2013 Subject: [Histonet] RE: PAX-8 background staining Ultra platform In-Reply-To: <598BB4A3A92D7F4DAA14C30E4AE70C2F10B1386E@MHSEXMB05.mhs.net> References: <598BB4A3A92D7F4DAA14C30E4AE70C2F10B1386E@MHSEXMB05.mhs.net> Message-ID: We use std cc1 Antibody incubation 36 min @ 36degrees WE use the PAX-8 that comes in the ventana dispenser clone MRQ-50...i think its actually a cell marquee one tho Vanessa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Obregon, Cecilia Sent: Tuesday, June 04, 2013 4:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] PAX-8 background staining Ultra platform Anyone running PAX-8 on the Benchmark Ultra succesfully? I have tried numerous protocols and antibodies, and still not able to obtain good results. I'm getting some background/cytoplasmic staining especially in normal kidney along with nuclear staining in the distal tubules and collecting ducts. I can't seem to completely be able to get it of it without compromising the signal. I have tried the Cell Marque MRQ-50 clone, the Biocare BC12, and now trying the Polyclonal Proteintech ...which looks promising. It's hard to duplicate the protocols that other institutions have in place mostly because the platforms are different. Our institution only has Ventana platform, and they're suggesting we try their new detection kit for this marker: Optiview. I can't honestly imagine buying a detection for just one antibody... We currently run UltraView Detection. Any inputs or suggestions would be greatly appreciated. Cecilia M. Obregon Memorial Regional Hospital 3501 Johnson Street Hollywood, FL 33021 Ph: 954-265-5317 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LSebree <@t> uwhealth.org Wed Jun 5 09:23:24 2013 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Wed Jun 5 09:23:29 2013 Subject: [Histonet] RE: PAX-8 background staining Ultra platform In-Reply-To: References: <598BB4A3A92D7F4DAA14C30E4AE70C2F10B1386E@MHSEXMB05.mhs.net> Message-ID: <77DD817201982748BC67D7960F2F76AF04B9A7@UWHC-MBX12.uwhis.hosp.wisc.edu> We also use Ventana's PAX-8, manufactured for VMS by Cell Marque. Our protocol on our Ultras is 36" CC1, 16" incubation @ 36 degrees C. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vanessa Perez Sent: Wednesday, June 05, 2013 8:47 AM To: Obregon, Cecilia; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: PAX-8 background staining Ultra platform We use std cc1 Antibody incubation 36 min @ 36degrees WE use the PAX-8 that comes in the ventana dispenser clone MRQ-50...i think its actually a cell marquee one tho Vanessa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Obregon, Cecilia Sent: Tuesday, June 04, 2013 4:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] PAX-8 background staining Ultra platform Anyone running PAX-8 on the Benchmark Ultra succesfully? I have tried numerous protocols and antibodies, and still not able to obtain good results. I'm getting some background/cytoplasmic staining especially in normal kidney along with nuclear staining in the distal tubules and collecting ducts. I can't seem to completely be able to get it of it without compromising the signal. I have tried the Cell Marque MRQ-50 clone, the Biocare BC12, and now trying the Polyclonal Proteintech ...which looks promising. It's hard to duplicate the protocols that other institutions have in place mostly because the platforms are different. Our institution only has Ventana platform, and they're suggesting we try their new detection kit for this marker: Optiview. I can't honestly imagine buying a detection for just one antibody... We currently run UltraView Detection. Any inputs or suggestions would be greatly appreciated. Cecilia M. Obregon Memorial Regional Hospital 3501 Johnson Street Hollywood, FL 33021 Ph: 954-265-5317 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BSullivan <@t> virtua.org Wed Jun 5 09:32:08 2013 From: BSullivan <@t> virtua.org (Sullivan, Beatrice) Date: Wed Jun 5 09:32:13 2013 Subject: [Histonet] Setting benchmark for Frozens Message-ID: <6932520047F7EE46B512E9801344F1603230B22C@ExchangeMB-1.Virtua.org> We are attempting to set a realistic benchmark for TAT on Frozen Sections. Any suggestions? We currently use 20 minutes without including multiple part cases. Thank you. Beatrice L. Sullivan HT(ASCP)HTL Corporate Histology Manager Virtua, Voorhees 856-247-3144 This message, and any included attachments, are from Virtua Health or its related affiliates and is intended only for the addressee(s). The information contained herein is privileged, proprietary or may include confidential information and/or protected patient health information. Any unauthorized review, forwarding, printing, copying, distributing, or otherwise disseminating or taking any action based on such information is strictly prohibited. If you have received this message in error, or have reason to believe you are not authorized to receive it, please delete this message promptly and notify the sender by e-mail with a copy to ISSECURITY@virtua.org. Thank you From cjohnson <@t> nmda.nmsu.edu Wed Jun 5 09:39:00 2013 From: cjohnson <@t> nmda.nmsu.edu (Johnson, Carole) Date: Wed Jun 5 09:39:13 2013 Subject: [Histonet] CD 79a Message-ID: Hello everyone. I am working up CD 79a (primarily on dogs and cats) and would like to know what folks in the veterinary world are successful with. I have been unsuccessful with Novocastra antibody. Has anyone got experience with the antibody form LS Bio? I use Bond Max platform. Thanks for your input! Carole Johnson, HT(ASCP)cm Histopathology Section Supervisor New Mexico Department of Agriculture Veterinary Diagnostic Services Confidentiality Notice: New Mexico has a very broad public records law. Most written communications to or from state employees are public records. Your e-mail communications may therefore be subject to public disclosure. This e-mail, including all attachments is for the sole use of the intended recipients. Any unauthorized review, use, disclosure or distribution is prohibited unless specifically provided under the New Mexico Inspection of Public Records Act. From shive003 <@t> umn.edu Wed Jun 5 09:54:51 2013 From: shive003 <@t> umn.edu (Jan Shivers) Date: Wed Jun 5 09:54:58 2013 Subject: [Histonet] CD 79a In-Reply-To: References: Message-ID: Carole, I use Biocare's CM067C (clone HM47/A9) with HIER in a Biocare Decloaking Chamber, pH 6.0 citrate buffer solution. It works successfully on cat, cow, deer, dog, dolphin, ferret, frog, goat, hedgehog, horse, llama, monkey, pig, rabbit, seal, and sheep. It does not work on chicken, eagle, or kangaroo. Jan Shivers Senior Scientist/Section Head - IHC/Histo U of MN Veterinary Diagnostic Lab On Wed, Jun 5, 2013 at 9:39 AM, Johnson, Carole wrote: > Hello everyone. I am working up CD 79a (primarily on dogs and cats) and > would like to know what folks in the veterinary world are successful with. > I have been unsuccessful with Novocastra antibody. Has anyone got > experience with the antibody form LS Bio? I use Bond Max platform. Thanks > for your input! > > Carole Johnson, HT(ASCP)cm > Histopathology Section Supervisor > New Mexico Department of Agriculture > Veterinary Diagnostic Services > > Confidentiality Notice: New Mexico has a very broad public records law. > Most written communications to or from state employees are public records. > Your e-mail communications may therefore be subject to public disclosure. > This e-mail, including all attachments is for the sole use of the intended > recipients. Any unauthorized review, use, disclosure or distribution is > prohibited unless specifically provided under the New Mexico Inspection of > Public Records Act. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From trathborne <@t> somerset-healthcare.com Wed Jun 5 10:41:31 2013 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Wed Jun 5 10:42:34 2013 Subject: [Histonet] RE: Setting benchmark for Frozens In-Reply-To: <6932520047F7EE46B512E9801344F1603230B22C@ExchangeMB-1.Virtua.org> References: <6932520047F7EE46B512E9801344F1603230B22C@ExchangeMB-1.Virtua.org> Message-ID: <3AD061FE740D464FAC7BF6B5CFB757076C6F8C1E@smcmail02.somerset-healthcare.com> We have been tracking this for a number of years. The CAP benchmark is 20 minutes, and that is what we had used when we first started. However, because we have had years when the average started to be closer to 17-18 minutes we set 2 standards - ours and CAP's. Right now we look to maintain a fifteen minute TAT, and are often around 12 minutes. I believe that our times are this good for a number of reasons. We are located in the same building as our OR. We use a pneumatic tube to expedite transportation. We have an "OR hotline" phone which was installed next to the tube system in the OR. All they have to do is pick up the hand-set and it will automatically ring at 5 different phones in Pathology. It will not go to any voice mails either. Someone is always able to answer in this way. We are then notified that a FS is on the way. This gives us time to start the pre-cool on the cryostat, notify the pathologist, log on to the computer and uncover the stain solutions. If by that time the specimen has not yet been delivered by the receiving person at the Lab end, we go to the tube and check for it ourselves. Even though the TAT is from Received to Called, we still find the time saved preparing before the actual receipt of the specimen to be beneficial. If you have problems meeting the 20 minute CAP benchmark, start by tracking your own times and try to improve each quarter. Look at processes and how simple changes can be made. Good luck! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sullivan, Beatrice Sent: Wednesday, June 05, 2013 10:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Setting benchmark for Frozens We are attempting to set a realistic benchmark for TAT on Frozen Sections. Any suggestions? We currently use 20 minutes without including multiple part cases. Thank you. Beatrice L. Sullivan HT(ASCP)HTL Corporate Histology Manager Virtua, Voorhees 856-247-3144 This message, and any included attachments, are from Virtua Health or its related affiliates and is intended only for the addressee(s). The information contained herein is privileged, proprietary or may include confidential information and/or protected patient health information. Any unauthorized review, forwarding, printing, copying, distributing, or otherwise disseminating or taking any action based on such information is strictly prohibited. If you have received this message in error, or have reason to believe you are not authorized to receive it, please delete this message promptly and notify the sender by e-mail with a copy to ISSECURITY@virtua.org. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BGapinski <@t> pathgroup.com Wed Jun 5 11:34:25 2013 From: BGapinski <@t> pathgroup.com (Bruce Gapinski) Date: Wed Jun 5 11:34:31 2013 Subject: [Histonet] 10% NBF non-hazardous Message-ID: Haha everyone! Some funny stuff out there for sure. Good thing you don't live here in California where 10% NBF is listed as a carcinogen. Proposition 65 (California's Safe Drinking Water Act circa 1970) here states that no carcinogen may go down the drain. Get this.... The only things that go down our drain are soap, water and bleach. And the cost isn't so great, after all we're still in business. I must admit I am proud of this. Hippy, Bruce Gapinsk HT (ASCP) Chief Histologist Marin Medical Laboratories PathGroup SF ________________________________ Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you From b427297 <@t> aol.com Wed Jun 5 11:47:53 2013 From: b427297 <@t> aol.com (Jackie O'Connor) Date: Wed Jun 5 11:47:59 2013 Subject: [Histonet] 10% NBF non-hazardous In-Reply-To: References: Message-ID: <8D030238E95FDC7-22C4-3F6D@webmail-d275.sysops.aol.com> To be clear, we don't pour it down the drain, we still collect waste (up to 40 gallons a week) in carboys and submit it for disposal through our company - we are a large company.. but when we submit chemical waste, the designation is "non-hazardous" on our website, which piqued my interest to notify our safety peeps. I just don't get how they determined this. -----Original Message----- From: Bruce Gapinski To: 'histonet@lists.utsouthwestern.edu' Sent: Wed, Jun 5, 2013 11:34 am Subject: [Histonet] 10% NBF non-hazardous Haha everyone! Some funny stuff out there for sure. Good thing you don't live here in California where 10% NBF is listed as a arcinogen. Proposition 65 (California's Safe Drinking Water Act circa 1970) ere states that no carcinogen may go down the drain. et this.... The only things that go down our drain are soap, water and bleach. nd the cost isn't so great, after all we're still in business. I must admit I m proud of this. ippy, ruce Gapinsk HT (ASCP) hief Histologist arin Medical Laboratories athGroup SF _______________________________ Important Notice: This e-mail is intended for the use of the person to whom it s addressed and may contain information that is privileged and confidential. If ou are not the intended recipient, any disclosure, copying, distribution, or se of the contents of this message is strictly prohibited. If you have received his e-mail in error, please destroy this message and contact the Security fficer at PathGroup, Inc immediately at 615-562-9255. Thank you ______________________________________________ istonet mailing list istonet@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet From cpyse <@t> x-celllab.com Wed Jun 5 11:47:59 2013 From: cpyse <@t> x-celllab.com (Cindy Pyse) Date: Wed Jun 5 11:48:04 2013 Subject: [Histonet] her-2-neu Message-ID: <002a01ce620c$6fd82950$4f887bf0$@x-celllab.com> Hello Histonetters Just recently we have been having some variable staining with the Her-2- Neu. We are using the Dako kit with the Dako stainer. I will get one case that the staining is light while the rest of the slides stain fine. We repeat the staining and the results are fine. No change in any reagents or buffer expect for the pretreatment that is made fresh daily. Any ideas? Any help will be appreciated. Thanks in advance. Cindy Cindy Pyse CLT, HT(ASCP) Laboratory Manager X-Cell Laboratories of WNY 20 Northpointe Parkway Ste 100 Amherst, NY 14228 716-250-9235 Ext. 232 cpyse@x-celllab.com From marktarango <@t> gmail.com Wed Jun 5 12:53:58 2013 From: marktarango <@t> gmail.com (Mark Tarango) Date: Wed Jun 5 12:54:02 2013 Subject: [Histonet] FISH controls In-Reply-To: References: Message-ID: Hi Liz, We do use cell line controls. We use HS578T for our cutoff control (in the equivocal range 1.8-2.2). This control usually scores at 1.8-2.0. We also use MDA-MB-231 as our negative control which usually gives a score of 1.0-1.1. Both of these can be purchased from ATCC. These are the same cell lines that are mentioned in the PathVysion HER2 FISH product insert as the controls that Abbott sells. You can purchase the cell line from ATCC, grow it up, and make your own cell blocks.. or purchase control slides from Abbott. We ran 55 cases during validation and got over 95% concordance with FISH performed at an outside lab. Mark On Wed, Jun 5, 2013 at 6:21 AM, Drinkall, Liz wrote: > Hi everyone, > > I am working in a brand new cytogenetics lab, and we have some questions > about controls for Her2. Does your lab use a control for each run, and if > so, what does your control contain? Have you ever used a Her2 positive cell > line as a control? Finally, during our validations for FISH, is it enough > to get the same result as Clarient or do we need to verify with another > method? Thanks in advance for any help you can give. > > Liz > > > > Liz Drinkall, HTL (ASCP) CM > Methodist Hospital > Histology Lab/Nebraska Collaborative Lab > 8303 Dodge St. > Omaha, NE 68114 > (402)354-4572/(402)354-7974 > > > This message and any included attachments are from Nebraska Methodist > Health System and its affiliates and are intended only for the addressee. > The message may contain privileged, confidential and/or proprietary > information intended only for the person(s) named. Unauthorized > forwarding, printing, copying, distribution, or use of such information is > strictly prohibited and may be unlawful. If you are not the addressee, > please promptly delete this message and notify the sender of the delivery > error by e-mail or you may call Nebraska Methodist Health System and its > affiliates in Omaha, Nebraska, U.S.A at (402)354-2280 > ._______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From rjbuesa <@t> yahoo.com Wed Jun 5 13:12:28 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jun 5 13:12:33 2013 Subject: [Histonet] Setting benchmark for Frozens In-Reply-To: <6932520047F7EE46B512E9801344F1603230B22C@ExchangeMB-1.Virtua.org> References: <6932520047F7EE46B512E9801344F1603230B22C@ExchangeMB-1.Virtua.org> Message-ID: <1370455948.70687.YahooMailNeo@web163102.mail.bf1.yahoo.com> I do not think you have to do much, because frozen TAT is the ONLY task the CAP has developed a standard of 20 minutes or less since the specimen is received at the lab. As an internal standard, the national average is 15 minutes but why push yourself? Just make sure that the TAT is 20 minutes and you will be OK with CAP Ren? J. From: "Sullivan, Beatrice" To: "histonet@lists.utsouthwestern.edu" Sent: Wednesday, June 5, 2013 10:32 AM Subject: [Histonet] Setting benchmark for Frozens We are attempting to set a realistic benchmark for TAT on Frozen Sections. Any suggestions? We currently use 20 minutes without including multiple part cases. Thank you. Beatrice L. Sullivan HT(ASCP)HTL Corporate Histology Manager Virtua, Voorhees 856-247-3144 This message, and any included attachments, are from Virtua Health or its related affiliates and is intended only for the addressee(s). The information contained herein is privileged, proprietary or may include confidential information and/or protected patient health information. Any unauthorized review, forwarding, printing, copying, distributing, or otherwise disseminating or taking any action based on such information is strictly prohibited. If you have received this message in error, or have reason to believe you are not authorized to receive it, please delete this message promptly and notify the sender by e-mail with a copy to ISSECURITY@virtua.org. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Karen.Heckford <@t> DignityHealth.org Thu Jun 6 09:12:25 2013 From: Karen.Heckford <@t> DignityHealth.org (Heckford, Karen - SMMC-SF) Date: Thu Jun 6 09:12:37 2013 Subject: [Histonet] Tissue Falling off Message-ID: Good Morning, I have a question about tissue falling off in the higher ph decloaking. I just tried using 10%NBF for 20 minutes after deparaffinizing and before decloaking. I still had the tissue fall off. It is well fixed breast tissue. Could I use albumin on the slide or will that interfere with the staining? Any suggestions welcomed or if you have another way of doing the formalin trick. I use a pressure cooker and it gets up to 126.5c for 5 minutes and then goes down to 89.5c for ten seconds. Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." From sarah_taba <@t> yahoo.com Thu Jun 6 09:27:45 2013 From: sarah_taba <@t> yahoo.com (sarah Tabatabaei) Date: Thu Jun 6 09:27:49 2013 Subject: [Histonet] Please unsubscribe. Message-ID: <1370528865.83006.YahooMailNeo@web160704.mail.bf1.yahoo.com> Thank you. From Bauer.Karen <@t> mayo.edu Thu Jun 6 09:35:52 2013 From: Bauer.Karen <@t> mayo.edu (Bauer, Karen L.) Date: Thu Jun 6 09:35:58 2013 Subject: [Histonet] RE: Setting benchmark for Frozens In-Reply-To: <6932520047F7EE46B512E9801344F1603230B22C@ExchangeMB-1.Virtua.org> References: <6932520047F7EE46B512E9801344F1603230B22C@ExchangeMB-1.Virtua.org> Message-ID: <8A2A8E67E35BB24DAD9375F9E163107507FFBD@MSGPEXCHA01A.mfad.mfroot.org> Hi Beatrice, FS TAT is no longer a CAP requirement, since ANP.11820 was deleted from the checklist in 2011. We still document an annual sample of FS TATs as part of our Quality Management measures. I believe CAP understood that all labs/departments are unique and should set standards specific to what works for them. FS TAT times should be set by the medical director and placed in FS procedures, but CAP is no longer listing that requirement in the ANP checklist. Good luck, Karen Karen L. Bauer HTL/HT (ASCP) | Histology Supervisor | Pathology | MOHS Lab Supervisor | Dermatology | Phone: 715-838-3205 | bauer.karen@mayo.edu | Mayo Clinic Health System | 1221 Whipple Street | Eau Claire, WI 54702 | mayoclinichealthsystem.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sullivan, Beatrice Sent: Wednesday, June 05, 2013 9:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Setting benchmark for Frozens We are attempting to set a realistic benchmark for TAT on Frozen Sections. Any suggestions? We currently use 20 minutes without including multiple part cases. Thank you. Beatrice L. Sullivan HT(ASCP)HTL Corporate Histology Manager Virtua, Voorhees 856-247-3144 This message, and any included attachments, are from Virtua Health or its related affiliates and is intended only for the addressee(s). The information contained herein is privileged, proprietary or may include confidential information and/or protected patient health information. Any unauthorized review, forwarding, printing, copying, distributing, or otherwise disseminating or taking any action based on such information is strictly prohibited. If you have received this message in error, or have reason to believe you are not authorized to receive it, please delete this message promptly and notify the sender by e-mail with a copy to ISSECURITY@virtua.org. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Jun 6 09:55:59 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jun 6 09:56:05 2013 Subject: [Histonet] Tissue Falling off In-Reply-To: References: Message-ID: <1370530559.35499.YahooMailNeo@web163105.mail.bf1.yahoo.com> And why would you use 10% NBF after deperaffinizing and before decloaking? Use (+) charged slides Ren? J. From: "Heckford, Karen - SMMC-SF" To: "histonet@lists.utsouthwestern.edu" Sent: Thursday, June 6, 2013 10:12 AM Subject: [Histonet] Tissue Falling off Good Morning, I have a question about tissue falling off in the higher ph decloaking.? ? I just tried using 10%NBF for 20 minutes after deparaffinizing and before decloaking.? I still had the tissue fall off.? It is well fixed breast tissue.? Could I use albumin on the slide or will that interfere with the staining?? ? Any suggestions welcomed or if you have another way of doing the formalin trick.? ? I use a pressure cooker and it gets up to 126.5c for 5 minutes and then goes down to 89.5c for ten seconds. Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford@dignityhealth.org ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? Caution:? This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED.? The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error.? Any further review, dissemination, distribution, or copying of this message is strictly prohibited.? If you have received this communication in error, please notify us? immediately by reply email.? Thank you." _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histotalk <@t> yahoo.com Thu Jun 6 10:36:49 2013 From: histotalk <@t> yahoo.com (David Kemler) Date: Thu Jun 6 10:36:56 2013 Subject: [Histonet] FW: breaking news Message-ID: <1370533009.92195.YahooMailNeo@web121506.mail.ne1.yahoo.com> http://www.aecapital.com/jxwke/qwro.fkmmwqngii From c.tague <@t> Pathologyarts.com Thu Jun 6 11:23:20 2013 From: c.tague <@t> Pathologyarts.com (Curt) Date: Thu Jun 6 11:23:32 2013 Subject: [Histonet] MMR IHC question Message-ID: <9C8F910F72893643B3C3793C3D67132B0139C623@PATHOLOGYSERVER.pathologyarts.local> Hello histo-world, This might not exactly be a histo question but I know you are all so knowledgeable, it couldn't hurt to ask. I'm looking to add some molecular testing to our menu, specifically reflex testing when there is an absence of a MMR protein, let's use Lynch syndrome as an example. First there is a pane of IHC (MLH1, MSH2, MSH6, PMS2), depending on the results the next step is reflex for molecular tests. My simple question is this, what type of equipment are these molecular test run on? Are there universal platforms that run molecular tests for a variety of tumors, the only variation would be the "kit" for that specific gene or set of genes? Does that make sense, what platform or machine is needed to run molecular tests? Thanks, Curt From asmith <@t> mail.barry.edu Thu Jun 6 12:16:36 2013 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Thu Jun 6 12:16:50 2013 Subject: [Histonet] RE: Tissue Falling off In-Reply-To: References: Message-ID: <93D1C752AF965C43AE4C84D6D45CFC2FDFFFD1@BL2PRD0710MB373.namprd07.prod.outlook.com> Use silane-treated slides: buy them of make your own with "Vectabond" or something similar. I have only once in 6 years lost a section off a silane-treated slide. - Allen A. Smith, Ph.D. Barry University School of Podiatric Medicine Miami Shores, Florida -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Heckford, Karen - SMMC-SF Sent: Thursday, June 06, 2013 10:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tissue Falling off Good Morning, I have a question about tissue falling off in the higher ph decloaking. I just tried using 10%NBF for 20 minutes after deparaffinizing and before decloaking. I still had the tissue fall off. It is well fixed breast tissue. Could I use albumin on the slide or will that interfere with the staining? Any suggestions welcomed or if you have another way of doing the formalin trick. I use a pressure cooker and it gets up to 126.5c for 5 minutes and then goes down to 89.5c for ten seconds. Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From marktarango <@t> gmail.com Thu Jun 6 12:54:50 2013 From: marktarango <@t> gmail.com (Mark Tarango) Date: Thu Jun 6 12:54:56 2013 Subject: [Histonet] MMR IHC question In-Reply-To: <9C8F910F72893643B3C3793C3D67132B0139C623@PATHOLOGYSERVER.pathologyarts.local> References: <9C8F910F72893643B3C3793C3D67132B0139C623@PATHOLOGYSERVER.pathologyarts.local> Message-ID: Hi Curt, You can setup these assays from scratch if you know what you're doing, but if you want FDA approved or to buy simple kits then you need to go with a company that sell that. Roche just recently had their EGFR assay FDA approved. We are using Qiagen products for EGFR, KRAS, BRAF. We use the QIAcube for DNA extraction, the QIAgility for liquid handling in preparation of master mixes, and the rotorgene for real-time PCR. There are different tests to reflex to at the point of MMR negative IHC staining. Some labs do BRAF and/or KRAS as a surrogate marker for sporadic CRC. Other labs might do methylation testing directly. We're currently validating a sequencing methylation assay for MLH-1 using Qiagen's pyrosequencing platform (PyroMark). You should definitely have a pathologist involved in this. Mark On Thu, Jun 6, 2013 at 9:23 AM, Curt wrote: > Hello histo-world, > > This might not exactly be a histo question but I know you are all so > knowledgeable, it couldn't hurt to ask. > > I'm looking to add some molecular testing to our menu, specifically reflex > testing when there is an absence of a MMR protein, let's use Lynch syndrome > as an example. First there is a pane of IHC (MLH1, MSH2, MSH6, PMS2), > depending on the results the next step is reflex for molecular tests. > My simple question is this, what type of equipment are these molecular > test run on? Are there universal platforms that run molecular tests for a > variety of tumors, the only variation would be the "kit" for that specific > gene or set of genes? > > Does that make sense, what platform or machine is needed to run molecular > tests? > > Thanks, > > Curt > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From richeyk <@t> musc.edu Thu Jun 6 13:02:46 2013 From: richeyk <@t> musc.edu (Richey, Katie) Date: Thu Jun 6 13:03:50 2013 Subject: [Histonet] Histology Technologist Needed!! Message-ID: <8A0408B04034CF47A315F652EF7479F714AA732B02@EVS7.clinlan.local> The Medical University of South Carolina is in need of a Histology Technologist. We have two vacancies - one in Histopathology & Special Stains and one in the MOHS' Surgery Lab. Please see our ad below and if interested please visit our website http://www.muscjobs.com and submit your application. HISTOLOGY TECHNOLOGIST NEEDED! The 700+ bed Medical Center is made up of four hospitals: Medical University Hospital, MUSC Children's Hospital, the Institute of Psychiatry, and the Ashley River Tower which is home to the Heart & Vascular Center and Digestive Disease Center. MUSC Medical Center also contains centers for specialized care: Transplant Center, Storm, Eye Institute, and the Hollings Cancer Center. Accredited by Joint Commission, MUSC has consistently received top rankings by US News and World Report. About our Team The MUSC Medical Center offers employees the opportunity for growth and development and to be recognized and rewarded for their achievements. Nurses, physicians, pharmacists, therapists, health care practitioners and other employees work side by side as strategic partners with a single goal: to provide the most effective patient service in the most efficient and caring manner. At the MUSC Medical Center, you will find excellence in patient care and rewarding careers. With Charleston's only Level One Trauma Center, Level III Neonatal Intensive Care Unit and Transplant Services, we are the leader in advanced health care. We are proud of our award-winning staff and the services they provide. Under direct supervision this individual will perform histologic procedures on human tissues to include but not limited to routine histology, special stains, Immunohistochemistry procedures, and frozen sectioning for microscopic examination by a pathologist. We offer a competitive compensation and benefits package in a progressive environment. All interested candidates should complete an online application at www.muscjobs.com. Please reference posting # A012438 to apply for this position. "Promoting Workplace Diversity: An Equal Opportunity Employer" Requirements: A bachelor's degree in histotechnology, biology or other related scientific discipline and histotechnologist HTL(ASCP) certification by the American Society of Clinical Pathology Board of Certification (ASCP BOC). If not certified, must be registry eligible and successfully obtain certification within the first year of employment. Katie Richey, MHA Healthcare Recruiter Medical University of South Carolina Medical Center Human Resources 163 Rutledge Ave, Suite 200 MSC 602 Charleston, SC 29425 Phone: 843.792.4491 Fax: 843.792.0853 From Karen.Heckford <@t> DignityHealth.org Thu Jun 6 13:35:35 2013 From: Karen.Heckford <@t> DignityHealth.org (Heckford, Karen - SMMC-SF) Date: Thu Jun 6 13:35:44 2013 Subject: [Histonet] Part time job in Berkeley California Message-ID: I was just informed by one of my Pathologists that they are looking for a part time Histology Tech at a small Urology lab in Berkeley near Alta Bates hospital. The pay is competitive for the area. If you are interested call Dr. Janet Kallo at: 415-269-7285. No recruiters please! Thanks, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." From tissuetech <@t> juno.com Thu Jun 6 13:38:53 2013 From: tissuetech <@t> juno.com (tissuetech@juno.com) Date: Thu Jun 6 13:39:25 2013 Subject: [Histonet] Histology Accessioning Position Open Message-ID: <20130606.133853.23072.0@webmail09.vgs.untd.com> Tissue Techniques is a small energetic laboratory with a family feel, and we are currently in search of an experienced Histology Data Entry team member. Tissue Techniques is located in Dallas, Texas. Applying future team members must have knowledge of common Histology terminology, and familiarity of Patient Demographic entry. Please send all interests to tissuetech@juno.com with a subject line of Accessioning Position. Thank you The Tissue Techniques Team ____________________________________________________________ BlackBerry® 10 Find out more about the new BlackBerry 10 smartphone. http://thirdpartyoffers.juno.com/TGL3141/51b0d74e363e9574d5eb6st03vuc From lmdee1 <@t> yahoo.com Thu Jun 6 14:24:32 2013 From: lmdee1 <@t> yahoo.com (Linda) Date: Thu Jun 6 14:24:36 2013 Subject: [Histonet] CAP inspect- It's not about you(lab), it is about me(inspector) Message-ID: <1370546672.87658.YahooMailNeo@web164003.mail.gq1.yahoo.com> Greetings All, ? I just went through my Physician Office Laboratory(POL) inspection recently. ? We all have to go through CAP inspections.? Yes, it is a voluntarily process.? We all work very hard and make sure everything is spit shined when they come into our pseudo-homes. ? With all the CAP rules changing, all the multitude of sections verses just Lab general, Anatomic Path, and if you do cytology, that section.?Now, with the non-exist?traditional hospital laboratory being replaced with a central location to serve many hospitals.? Or now, as am I, a physical owned lab, with no pathologist on site.? And as always, being understaffed, required to produce more in a shorter amount of time, etc., essentially not feeling the "love". ? Do you feel the laboratory inspection process should change too? ? I am not endorsing or refuting any of the suggested process changes,?they are?thoughts?that I actually have heard through the society grapevine.? Such as being taken over by the FDA, or having paid inspection teams, or some other process in-between?? A different set of inspectors who understand that POLs,? are the test(analytical) process and not the post-analytical process(the path report). ? I send out to histoland the question interested in your thoughts. ? Respectfully, ? Linda Dee, BGS, HT(ASCP)? From Timothy.Morken <@t> ucsfmedctr.org Thu Jun 6 14:45:04 2013 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Thu Jun 6 14:45:19 2013 Subject: [Histonet] CAP inspect- It's not about you(lab), it is about me(inspector) In-Reply-To: <1370546672.87658.YahooMailNeo@web164003.mail.gq1.yahoo.com> References: <1370546672.87658.YahooMailNeo@web164003.mail.gq1.yahoo.com> Message-ID: <761E2B5697F795489C8710BCC72141FF083047@ex07.net.ucsf.edu> FDA cannot inspect a lab that only uses commercially available reagents for their testing. FDA only has authority over the manufacturers of reagents, and even then only the "active ingredient," ie, the Anylate Specific Reagent. However, FDA is interested because so many labs are making up their own tests, mainly for genetic/molecular tho even the Her2 interpretation and test variability issues attract attention from FDA. Are labs really qualified to do high quality validation testing? That is what FDA is curious about....and should a lab be considered a "manufacturing" facility if they make up their own test, especially if they make their own genetic probes. All the rest you mention is under the purview of the deemed agents for CLIA - CAP and Joint Commission. They both inspect to ensure the CLIA regulations are followed, but use different approaches towards the same end. As more variety of testing is implemented, they have to interpret how the regulations apply to the new tests. CLIA has to sign off on what they do, so it is not something done without oversight. It could be that POL's are pushing the limits on what they can handle with the personnel they have. At our institution we have a large support staff for quality assurance and regulatory compliance. They keep track and work on a lot of the issues so I don't have to spend my time doing that. A POL would need to hire consultants to get the same level of support. I suspect many can't afford that so have to scrape by figuring it out for themselves. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Linda Sent: Thursday, June 06, 2013 12:25 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP inspect- It's not about you(lab), it is about me(inspector) Greetings All, ? I just went through my Physician Office Laboratory(POL) inspection recently. ? We all have to go through CAP inspections.? Yes, it is a voluntarily process.? We all work very hard and make sure everything is spit shined when they come into our pseudo-homes. ? With all the CAP rules changing, all the multitude of sections verses just Lab general, Anatomic Path, and if you do cytology, that section.?Now, with the non-exist?traditional hospital laboratory being replaced with a central location to serve many hospitals.? Or now, as am I, a physical owned lab, with no pathologist on site.? And as always, being understaffed, required to produce more in a shorter amount of time, etc., essentially not feeling the "love". ? Do you feel the laboratory inspection process should change too? ? I am not endorsing or refuting any of the suggested process changes,?they are?thoughts?that I actually have heard through the society grapevine.? Such as being taken over by the FDA, or having paid inspection teams, or some other process in-between?? A different set of inspectors who understand that POLs,? are the test(analytical) process and not the post-analytical process(the path report). ? I send out to histoland the question interested in your thoughts. ? Respectfully, ? Linda Dee, BGS, HT(ASCP)? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From c.tague <@t> Pathologyarts.com Thu Jun 6 14:51:48 2013 From: c.tague <@t> Pathologyarts.com (Curt) Date: Thu Jun 6 14:51:55 2013 Subject: [Histonet] CAP inspect- It's not about you(lab), it is about me(inspector) In-Reply-To: <1370546672.87658.YahooMailNeo@web164003.mail.gq1.yahoo.com> References: <1370546672.87658.YahooMailNeo@web164003.mail.gq1.yahoo.com> Message-ID: <9C8F910F72893643B3C3793C3D67132B0139CBA3@PATHOLOGYSERVER.pathologyarts.local> Though I don't think this is exactly what you're asking, I think the best thing they could do is terminate this "exception" to the law that allows preferential self referral. There is abusive over utilization costing millions/billions in additional cost to tax payers and those who purchase health insurance. The response from the feds is to cut the bread winning cpt code by 52% (in our area)... I have a friend who owns a small lab with 2 employees, because of these cuts he needs to lay them both off.... because of greedy POD's abusing the system. So there you have it, that's the first thing they should do. Curt -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Linda Sent: Thursday, June 06, 2013 12:25 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP inspect- It's not about you(lab), it is about me(inspector) Greetings All, ? I just went through my Physician Office Laboratory(POL) inspection recently. ? We all have to go through CAP inspections.? Yes, it is a voluntarily process.? We all work very hard and make sure everything is spit shined when they come into our pseudo-homes. ? With all the CAP rules changing, all the multitude of sections verses just Lab general, Anatomic Path, and if you do cytology, that section.?Now, with the non-exist?traditional hospital laboratory being replaced with a central location to serve many hospitals.? Or now, as am I, a physical owned lab, with no pathologist on site.? And as always, being understaffed, required to produce more in a shorter amount of time, etc., essentially not feeling the "love". ? Do you feel the laboratory inspection process should change too? ? I am not endorsing or refuting any of the suggested process changes,?they are?thoughts?that I actually have heard through the society grapevine.? Such as being taken over by the FDA, or having paid inspection teams, or some other process in-between?? A different set of inspectors who understand that POLs,? are the test(analytical) process and not the post-analytical process(the path report). ? I send out to histoland the question interested in your thoughts. ? Respectfully, ? Linda Dee, BGS, HT(ASCP)? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Thu Jun 6 15:19:56 2013 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Thu Jun 6 15:20:03 2013 Subject: [Histonet] CAP inspect- It's not about you(lab), it is about me(inspector) In-Reply-To: <761E2B5697F795489C8710BCC72141FF083047@ex07.net.ucsf.edu> References: <1370546672.87658.YahooMailNeo@web164003.mail.gq1.yahoo.com> <761E2B5697F795489C8710BCC72141FF083047@ex07.net.ucsf.edu> Message-ID: <14E2C6176416974295479C64A11CB9AE016411AC2FA1@SBS2K8.premierlab.local> Tim I'm not sure you are completely accurate, maybe in the clinical laboratory, but the FDA does inspect labs, my lab has been inspected by the FDA back in 2007 and I suspect we will have another inspection soon, these inspections are not planned, they just show up, which can be quite exciting. The inspection I had was related to GLP compliance. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Laboratory Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 Work (303) 682-3949 Fax (303) 682-9060 Cell (303) 881-0763 liz@premierlab.com www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Thursday, June 06, 2013 1:45 PM To: Linda; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CAP inspect- It's not about you(lab), it is about me(inspector) FDA cannot inspect a lab that only uses commercially available reagents for their testing. FDA only has authority over the manufacturers of reagents, and even then only the "active ingredient," ie, the Anylate Specific Reagent. However, FDA is interested because so many labs are making up their own tests, mainly for genetic/molecular tho even the Her2 interpretation and test variability issues attract attention from FDA. Are labs really qualified to do high quality validation testing? That is what FDA is curious about....and should a lab be considered a "manufacturing" facility if they make up their own test, especially if they make their own genetic probes. All the rest you mention is under the purview of the deemed agents for CLIA - CAP and Joint Commission. They both inspect to ensure the CLIA regulations are followed, but use different approaches towards the same end. As more variety of testing is implemented, they have to interpret how the regulations apply to the new tests. CLIA has to sign off on what they do, so it is not something done without oversight. It could be that POL's are pushing the limits on what they can handle with the personnel they have. At our institution we have a large support staff for quality assurance and regulatory compliance. They keep track and work on a lot of the issues so I don't have to spend my time doing that. A POL would need to hire consultants to get the same level of support. I suspect many can't afford that so have to scrape by figuring it out for themselves. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Linda Sent: Thursday, June 06, 2013 12:25 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP inspect- It's not about you(lab), it is about me(inspector) Greetings All, I just went through my Physician Office Laboratory(POL) inspection recently. We all have to go through CAP inspections. Yes, it is a voluntarily process. We all work very hard and make sure everything is spit shined when they come into our pseudo-homes. With all the CAP rules changing, all the multitude of sections verses just Lab general, Anatomic Path, and if you do cytology, that section. Now, with the non-exist traditional hospital laboratory being replaced with a central location to serve many hospitals. Or now, as am I, a physical owned lab, with no pathologist on site. And as always, being understaffed, required to produce more in a shorter amount of time, etc., essentially not feeling the "love". Do you feel the laboratory inspection process should change too? I am not endorsing or refuting any of the suggested process changes, they are thoughts that I actually have heard through the society grapevine. Such as being taken over by the FDA, or having paid inspection teams, or some other process in-between? A different set of inspectors who understand that POLs, are the test(analytical) process and not the post-analytical process(the path report). I send out to histoland the question interested in your thoughts. Respectfully, Linda Dee, BGS, HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Thu Jun 6 15:33:30 2013 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Thu Jun 6 15:33:44 2013 Subject: [Histonet] CAP inspect- It's not about you(lab), it is about me(inspector) In-Reply-To: <14E2C6176416974295479C64A11CB9AE016411AC2FA1@SBS2K8.premierlab.local> References: <1370546672.87658.YahooMailNeo@web164003.mail.gq1.yahoo.com> <761E2B5697F795489C8710BCC72141FF083047@ex07.net.ucsf.edu> <14E2C6176416974295479C64A11CB9AE016411AC2FA1@SBS2K8.premierlab.local> Message-ID: <761E2B5697F795489C8710BCC72141FF0830C6@ex07.net.ucsf.edu> Liz, That's because some (most?) of your work is related to drug or other product development. They do inspect GLP labs. They don't inspect purely diagnostic labs. At least human diagnostics. I'm not sure about veterinary diagnostics. Tim -----Original Message----- From: Elizabeth Chlipala [mailto:liz@premierlab.com] Sent: Thursday, June 06, 2013 1:20 PM To: Morken, Timothy; Linda; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CAP inspect- It's not about you(lab), it is about me(inspector) Tim I'm not sure you are completely accurate, maybe in the clinical laboratory, but the FDA does inspect labs, my lab has been inspected by the FDA back in 2007 and I suspect we will have another inspection soon, these inspections are not planned, they just show up, which can be quite exciting. The inspection I had was related to GLP compliance. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Laboratory Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 Work (303) 682-3949 Fax (303) 682-9060 Cell (303) 881-0763 liz@premierlab.com www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Thursday, June 06, 2013 1:45 PM To: Linda; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CAP inspect- It's not about you(lab), it is about me(inspector) FDA cannot inspect a lab that only uses commercially available reagents for their testing. FDA only has authority over the manufacturers of reagents, and even then only the "active ingredient," ie, the Anylate Specific Reagent. However, FDA is interested because so many labs are making up their own tests, mainly for genetic/molecular tho even the Her2 interpretation and test variability issues attract attention from FDA. Are labs really qualified to do high quality validation testing? That is what FDA is curious about....and should a lab be considered a "manufacturing" facility if they make up their own test, especially if they make their own genetic probes. All the rest you mention is under the purview of the deemed agents for CLIA - CAP and Joint Commission. They both inspect to ensure the CLIA regulations are followed, but use different approaches towards the same end. As more variety of testing is implemented, they have to interpret how the regulations apply to the new tests. CLIA has to sign off on what they do, so it is not something done without oversight. It could be that POL's are pushing the limits on what they can handle with the personnel they have. At our institution we have a large support staff for quality assurance and regulatory compliance. They keep track and work on a lot of the issues so I don't have to spend my time doing that. A POL would need to hire consultants to get the same level of support. I suspect many can't afford that so have to scrape by figuring it out for themselves. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Linda Sent: Thursday, June 06, 2013 12:25 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP inspect- It's not about you(lab), it is about me(inspector) Greetings All, I just went through my Physician Office Laboratory(POL) inspection recently. We all have to go through CAP inspections. Yes, it is a voluntarily process. We all work very hard and make sure everything is spit shined when they come into our pseudo-homes. With all the CAP rules changing, all the multitude of sections verses just Lab general, Anatomic Path, and if you do cytology, that section. Now, with the non-exist traditional hospital laboratory being replaced with a central location to serve many hospitals. Or now, as am I, a physical owned lab, with no pathologist on site. And as always, being understaffed, required to produce more in a shorter amount of time, etc., essentially not feeling the "love". Do you feel the laboratory inspection process should change too? I am not endorsing or refuting any of the suggested process changes, they are thoughts that I actually have heard through the society grapevine. Such as being taken over by the FDA, or having paid inspection teams, or some other process in-between? A different set of inspectors who understand that POLs, are the test(analytical) process and not the post-analytical process(the path report). I send out to histoland the question interested in your thoughts. Respectfully, Linda Dee, BGS, HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From fslovinsky <@t> yahoo.com Thu Jun 6 22:13:56 2013 From: fslovinsky <@t> yahoo.com (Frank Slovinsky) Date: Thu Jun 6 22:14:03 2013 Subject: [Histonet] salutations Message-ID: <1370574836.27755.YahooMailNeo@web161506.mail.bf1.yahoo.com> http://www.atonement-denver.org/tmp/youtube.php?jsroxvns882vdlzaip fslovinsky Frank Slovinsky ................ All the cool things in life can be described with either yes! or heh. -- Bruce Sherrod From jlm_327 <@t> hotmail.com Fri Jun 7 03:24:09 2013 From: jlm_327 <@t> hotmail.com (Jessica Meininger) Date: Fri Jun 7 03:24:20 2013 Subject: [Histonet] Pathologists' Assistant Message-ID: I know that this is mostly for histotechs and other related laboratory professionals, so I thought I would see if there are any lab professionals out there who have heard of a possible Pathologists' Assistant job at the hospital they are at. I will be graduating from a NAACLS accredited Pathologists' Assistant Program in mid-july. I will at that time be ASCP certification eligible. I am willing to relocate (from Michigan). If anyone has heard anything, and could give me some information, please let me know. Thank you so much. Sincerely, Jessica jlm_327@hotmail.com From SHUNTER <@t> beaumont.edu Fri Jun 7 07:16:42 2013 From: SHUNTER <@t> beaumont.edu (Sue Hunter) Date: Fri Jun 7 07:16:51 2013 Subject: [Histonet] MMR IHC question In-Reply-To: References: <9C8F910F72893643B3C3793C3D67132B0139C623@PATHOLOGYSERVER.pathologyarts.local> Message-ID: Hi Curt I agree with Mark - you can set up lab developed tests or - depending on your volumn, experience, requirements for FDA approval, etc - you can buy kits. We often reflex to MSI testing - run on capillary electrophoresis instruments- we have ABI-310s. Our primer sets are lab developed but you can also buy them. We also do KRAS, BRAF, EGFR(mostly on lungs), and MLH1 methylation by real time on an ABI 7500. Easy to set up and run and fast run time. Kits are from EntroGen, MLH1 is lab developed. We do manual DNA extractions using the Qiagen extraction kits. Hope this helps. Sue Sue Hunter, Supervisor Advanced Diagnostics Beaumont Health System Royal Oak MI 248-898-5146 shunter@beaumont.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Tarango Sent: Thursday, June 06, 2013 1:55 PM To: Curt Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] MMR IHC question Hi Curt, You can setup these assays from scratch if you know what you're doing, but if you want FDA approved or to buy simple kits then you need to go with a company that sell that. Roche just recently had their EGFR assay FDA approved. We are using Qiagen products for EGFR, KRAS, BRAF. We use the QIAcube for DNA extraction, the QIAgility for liquid handling in preparation of master mixes, and the rotorgene for real-time PCR. There are different tests to reflex to at the point of MMR negative IHC staining. Some labs do BRAF and/or KRAS as a surrogate marker for sporadic CRC. Other labs might do methylation testing directly. We're currently validating a sequencing methylation assay for MLH-1 using Qiagen's pyrosequencing platform (PyroMark). You should definitely have a pathologist involved in this. Mark On Thu, Jun 6, 2013 at 9:23 AM, Curt wrote: > Hello histo-world, > > This might not exactly be a histo question but I know you are all so > knowledgeable, it couldn't hurt to ask. > > I'm looking to add some molecular testing to our menu, specifically > reflex testing when there is an absence of a MMR protein, let's use > Lynch syndrome as an example. First there is a pane of IHC (MLH1, > MSH2, MSH6, PMS2), depending on the results the next step is reflex for molecular tests. > My simple question is this, what type of equipment are these molecular > test run on? Are there universal platforms that run molecular tests > for a variety of tumors, the only variation would be the "kit" for > that specific gene or set of genes? > > Does that make sense, what platform or machine is needed to run > molecular tests? > > Thanks, > > Curt > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tkngflght <@t> yahoo.com Fri Jun 7 11:24:13 2013 From: tkngflght <@t> yahoo.com (Cheryl) Date: Fri Jun 7 11:24:18 2013 Subject: [Histonet] -70 freezer available for re-sale Message-ID: <1370622253.97956.YahooMailClassic@web161906.mail.bf1.yahoo.com> Hi All, ? We have a -70 freezer we no longer need--large upright model with double gaskets.? Very clean.? Was hoping to be called by refurbished equipment folks who might be interested.? ? SP Brand Cryo-fridge Series by Allegiance. Model 214L-560315-NM ? It's about 10+ years old. ? Please call 225.766.4999 and ask for me. Cheryl? From ahorvath <@t> cogipath.com Fri Jun 7 12:51:48 2013 From: ahorvath <@t> cogipath.com (andrew horvath) Date: Fri Jun 7 12:51:56 2013 Subject: [Histonet] high complexity testing and personnel requirements Message-ID: Hello Everyone, I am trying to verify if CLIA has changed their designation of who can perform high complexity testing. Recently I was informed that in the most recent CLIA regs (updated January 2013?) that the education requirements have been changed and made less restrictive. Those with a Bachelor degree, regardless if it was a science field, can gross. I have contacted a state CLIA contact but have not gotten a response yet. Can anyone confirm this change? thanks Andrew Horvath, ****MBA**, **MA******** Interim Operations Manager**** 303-770-4848 (O)**** 303-770-6641 (fax)**** **** *****Colorado****** GI Pathology***** ****7346 S. Alton Way******** ****Suite** 10E****** ****Centennial**, **CO** **80112 From jbro22 <@t> lsuhsc.edu Fri Jun 7 12:53:28 2013 From: jbro22 <@t> lsuhsc.edu (Browning, Jeffrey A.) Date: Fri Jun 7 12:53:34 2013 Subject: [Histonet] Pathology Assistant Position - Shreveport, LA Message-ID: <2E3D5719BC42374E982EF0AD611F236286F49EA2@SH-ExchMB1.master.lsuhsc.edu> Greetings, We have an upcoming vacancy for a Pathologist Assistant position in Shreveport, LA. Primary duties include grossing biopsies and large specimens (appendages, organs, etc) and a teaching role for pathology residents performing grossing rotations. Completion of an accredited Pathology Assistant program is NOT required. Candidates must possess: a bachelor degree in a physical science, completion of an accredited lab science program (HT, PA, etc), OR the educational equivalent--experience in anatomic pathology/grossing preferred. If you are interested or know anyone who may be please email me directly. Thanks! Jeff Browning, HTL(ASCP) Technical Director, Anatomic Pathology Department of Pathology LSU Health Science Center - Shreveport 1501 Kings Highway Shreveport, LA 71103 (318) 675-5872 jbro22@lsuhsc.edu From Reuel.Cornelia <@t> tsrh.org Fri Jun 7 14:22:31 2013 From: Reuel.Cornelia <@t> tsrh.org (Reuel Cornelia) Date: Fri Jun 7 14:22:41 2013 Subject: [Histonet] Thermo Scientific STP 420ES Message-ID: <51B1ECA7.077E.00C5.1@tsrh.org> Histonetters, Is anybody using a Thermo Scientific STP 420ES? What do you think of the processor. Please give us your feedback. Thank you. Reuel Cornelia From meganb1103 <@t> yahoo.com Fri Jun 7 18:18:50 2013 From: meganb1103 <@t> yahoo.com (Megan) Date: Fri Jun 7 18:18:54 2013 Subject: [Histonet] Hello Message-ID: <1370647130.51903.YahooMailClassic@web162403.mail.bf1.yahoo.com> Hello, I am a student in Histotechnology school and I am doing a research of the history of histology for the years 1826-1850 and I can not find any information online. Could someone point me to a few websites? Thanks so much. Megan From b427297 <@t> aol.com Fri Jun 7 21:56:03 2013 From: b427297 <@t> aol.com (b427297@aol.com) Date: Fri Jun 7 21:56:20 2013 Subject: [Histonet] Hello In-Reply-To: <1370647130.51903.YahooMailClassic@web162403.mail.bf1.yahoo.com> References: <1370647130.51903.YahooMailClassic@web162403.mail.bf1.yahoo.com> Message-ID: Google Dr James McCormick in Chicago. He knows everything about antique histo practices. He does lectures about it. I think he was there when the microscope was invented... :-) Sent from my iPhone On Jun 7, 2013, at 6:18 PM, Megan wrote: > Hello, > > I am a student in Histotechnology school and I am doing a research of the history of histology for the years 1826-1850 and I can not find any information online. Could someone point me to a few websites? Thanks so much. > > Megan > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From andreahooper <@t> rocketmail.com Sat Jun 8 07:23:11 2013 From: andreahooper <@t> rocketmail.com (Andrea Hooper) Date: Sat Jun 8 07:25:56 2013 Subject: [Histonet] CD3 Message-ID: <7F3C4208-9749-481C-AD55-AFF264212C87@rocketmail.com> Does anyone know if a human specific (no cross to mouse) anti-CD3 for paraffin? Thanks, Andrea From jdooley2008 <@t> yahoo.com Sat Jun 8 11:38:16 2013 From: jdooley2008 <@t> yahoo.com (James Dooley) Date: Sat Jun 8 11:38:20 2013 Subject: [Histonet] from: James Dooley subject: Message-ID: <1370709496.75407.YahooMailNeo@web120606.mail.ne1.yahoo.com> http://www.cmsgrid.net/izgkpndo.php?cgupdhesvv=dlighxtrhxok From bakevictoria <@t> gmail.com Sun Jun 9 14:32:39 2013 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Sun Jun 9 14:32:45 2013 Subject: [Histonet] Hello In-Reply-To: <1370647130.51903.YahooMailClassic@web162403.mail.bf1.yahoo.com> References: <1370647130.51903.YahooMailClassic@web162403.mail.bf1.yahoo.com> Message-ID: Hi Megan I don't have any direct websites, one of the best sources the AFIP closed in 2011 - they had a wonderful collection of artifacts and specimens going back to before the Civil War. (I have to confess I could have done without seeing Dan Sickles leg though - that was just plain creepy.) It was a wealth of information about the progression of Pathology in the time period you are asking about. Now it is a part of the Joint Pathology Center in Bethesda Maryland. The phone number is 301-295-4819. Another resource would be the NLM (National Library of Medicine) also in Bethesda. If you go to the website http://www.nlm.nih.gov/nmd/index.html it brings up a menu and if you go to Medical History Library you can start searching there. They have a group of reference librarians there that should be able to help you as well, the phone number is 301-402-8878 or you can e-mail them at hmdref@nlm.nih.gov . They were very helpful to me while I was at the NIH and if you tell them exactly what you are looking for I'm sure they can assist you with your project. Best of Luck Vikki Baker On Fri, Jun 7, 2013 at 7:18 PM, Megan wrote > Hello, > > I am a student in Histotechnology school and I am doing a research of the > history of histology for the years 1826-1850 and I can not find any > information online. Could someone point me to a few websites? Thanks so > much. > > Megan > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Dorothy.L.Webb <@t> HealthPartners.Com Mon Jun 10 12:24:04 2013 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Mon Jun 10 12:24:21 2013 Subject: [Histonet] RE: samples In-Reply-To: <20130610102005.329d3e5ba3e6f0f6c4282a5937147d9c.c0cdd02b11.wbe@email02.secureserver.net> References: <20130610102005.329d3e5ba3e6f0f6c4282a5937147d9c.c0cdd02b11.wbe@email02.secureserver.net> Message-ID: <65365F35C0F2EF4D846EC3CA73E49C4302758CF69446@HPEMX3.HealthPartners.int> It is actually not a website but the internet communication (like a listserve) they have for PA?s. For instance, histology has one at histonet@lists.utsouthwestern.edu that is used to communicate and network online. Thanks again! Dorothy Webb From: sales@royalmarker.com [mailto:sales@royalmarker.com] Sent: Monday, June 10, 2013 12:20 PM To: Webb, Dorothy L Subject: RE: samples No problem, the samples are on the way. How were you recommended for our product? Could you let us know which website you are referring to? Thanks Again, Shawn Jenkins -------- Original Message -------- Subject: RE: samples From: "Webb, Dorothy L" > Date: Fri, June 07, 2013 1:40 pm To: "'sales@royalmarker.com'" > Thank you very much. Your marking inks were recommended on the website for PA?s. Your efficient response is greatly appreciated and we will get back to you next week with feedback! Dorothy Webb From: sales@royalmarker.com [mailto:sales@royalmarker.com] Sent: Friday, June 07, 2013 12:30 PM To: Webb, Dorothy L Subject: RE: samples Hello Dorothy, We will gladly send you a sample pack of our marking dyes to try. It will ship out today via UPS to the address below that you provided. I'll be sure to have your name as well as Jesse Spates on the label as well for a contact. The set will include 10 1/4oz bottles (all of our colors). Please contact us with any comments on the product. We appreciate your feedback and hope you are happy with our products. Have a great weekend and look forward to hearing from you. Best Regards, Shawn Jenkins Royal Marker (P) 954-462-0264 (F) 954-374-7290 -------- Original Message -------- Subject: samples From: "Webb, Dorothy L" > Date: Fri, June 07, 2013 11:59 am To: "'sales@royalmarker.com'" > Cc: "Spates, Jesse D" > Our PA?s are looking to try other inks than what we are currently using so we are wondering if we could please get some ink samples. Our hospital is Regions Hospital 640 Jackson St. St. Paul, MN. 55101 I am the technical specialist of histology, Dorothy Webb and the lead PA is Jesse Spates @ 651-254-4913. Thank you, Dorothy Webb Regions Histology Technical Specialist 651-254-2962 ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 From wgray19 <@t> sc.rr.com Mon Jun 10 17:00:12 2013 From: wgray19 <@t> sc.rr.com (Wanda Shotsberger Gray) Date: Mon Jun 10 17:00:21 2013 Subject: [Histonet] Re: Histonet Digest, Vol 115, Issue 10 Message-ID: Hi Megan, Welcome to the anatomic side! I just googled "history of tissue dyes" and a wealth of information popped up including this PDF: www.immunologie-labor.com/cellmarker_files/IET_reagents_07.pdf all about the discovery of hematoxylin and aniline dyes. The time period you mentioned was the time of these discoveries, so searching for history of hematoxylin or eosin should also get you good hits. Good luck! Wanda Shotsberger HT/HTL (ASCP) From cfitz <@t> 007group.com Mon Jun 10 19:03:14 2013 From: cfitz <@t> 007group.com (Cathy) Date: Mon Jun 10 19:03:20 2013 Subject: [Histonet] bone marrow aspirations In-Reply-To: <38E00305B4744EDB9C25EE966E2030D9@JoannePC> References: <38E00305B4744EDB9C25EE966E2030D9@JoannePC> Message-ID: <001b01ce6637$11eb70c0$35c25240$@007group.com> We are using a similar method at our site. The aspirate is placed into EDTA and then into formalin to fix. We pour the aspirate through a biopsy bag, open the bag and scrape the spicules together, transfer them to another biopsy bag and process as usual. I have received several compliments on the quality of the slides. Cathy Kelowna, B.C. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shea's Sent: Saturday, June 1, 2013 4:26 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] bone marrow aspirations I am also interested in how others are processing bone marrow aspirates. Currently, we let the aspirate clot on a watch glass for about 20 minutes, gentle slide it onto filter paper, roll it around to get rid of the excess fluid, place the clot between sponges, and process as usual. However, I am interested in knowing if any one out there is using a technique that we use to use at another hospital 33 years ago. If I remember correctly, the pathologist or oncologist would collect the aspirate using a heparin syringe. Some how, the aspirate smears for Hematology were made on 22x22 cover slips instead of slides. Then the EDTA tube was then given to Histology. I can't remember if we added formalin or B5 fixative to it at that time, but it was filtered into an obex tea bag type filter paper (The blood never clotted and ran through the filter paper, leaving only bone marrow spicules in the paper). We would fold the paper, place it in the cassette, and process as usual. The pathologist preferred this method because he wouldn't have to look at levels of clot to find spicules. Is there anyone out there using this method? Jan _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Robert.Fauck <@t> ccdhb.org.nz Tue Jun 11 00:04:35 2013 From: Robert.Fauck <@t> ccdhb.org.nz (Robert Fauck [CCDHB]) Date: Tue Jun 11 00:05:03 2013 Subject: [Histonet] Her-2 antibody Message-ID: <9C92DFB6EA9983408AA9785182E9FC966804DE@WN0NTEML11.AD.CCDHB.HEALTH.NZ> Hi All We here in wellington Hospital New Zealand having some problem with the Her-2 ( Lieca, NCL-L-CBE-356 ) Top half of the slide ( control or Test tissue) are strongly positive but if we put the control tissue on the bottom half it is week to negative! Does any one has the same problems with your Her-2, it is done on a Leica Bond (Max) III. Much appreciated for any suggestions, also from Leica Technical specialists. Regards, Robert Fauck Wellington hospital, NZ This email or attachment(s) may contain confidential or legally privileged information intended for the sole use of the addressee(s). Any use, redistribution, disclosure, or reproduction of this message, except as intended, is prohibited. If you received this email in error, please notify the sender and remove all copies of the message, including any attachments. Any views or opinions expressed in this email (unless otherwise stated) may not represent those of Capital & Coast District Health Board. http://www.ccdhb.org.nz (1C_S1) From ione.jackman <@t> uconn.edu Tue Jun 11 08:34:09 2013 From: ione.jackman <@t> uconn.edu (Jackman, Mary Ione) Date: Tue Jun 11 08:34:14 2013 Subject: [Histonet] Heinz body stain on already dried blood smears. Message-ID: Good Morning, Does anyone have a protocol they like for demonstrating Heinz bodies on already dried blood smears? There is no blood in tubes available any longer. I saw a citation for using Wrights Giemsa, but is there one that is more specific that anyone uses and is willing to share? Thanks. Ione Jackman From gtharp <@t> pcasoutheast.com Tue Jun 11 09:08:40 2013 From: gtharp <@t> pcasoutheast.com (Gloria Tharp) Date: Tue Jun 11 09:08:55 2013 Subject: [Histonet] Telepathology Message-ID: <006701ce66ad$2c971fc0$85c55f40$@com> Does anyone have any recommendations for scanning equipment to have slides read off site from main lab. Thanks. Gloria Tharp, BA, HTL(ASCP) Director of Operations PCA Southeast gtharp@pcasoutheast.com 931-490-1005 800-388-1294 From liz <@t> premierlab.com Tue Jun 11 09:28:20 2013 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Tue Jun 11 09:28:27 2013 Subject: [Histonet] Telepathology In-Reply-To: <006701ce66ad$2c971fc0$85c55f40$@com> References: <006701ce66ad$2c971fc0$85c55f40$@com> Message-ID: <14E2C6176416974295479C64A11CB9AE016411AB4898@SBS2K8.premierlab.local> Gloria There are many systems out there that perform scanning, your choice is dependent upon how you are going to be utilizing the instrument, how many slides per day that you will need to scan, if you will be utilizing any image analysis algorithms, etc. The DPA has lots information on its website - https://digitalpathologyassociation.org/ They are also hosting a free webinar on July 10th (this is the 1st of 4) to sign up just go to this link https://digitalpathologyassociation.org/2013-dpa-webinar-series The webinar is titled Real-World Examples of Practical Consultative/Collaborative Digital Pathology Programs Wednesday, July 10 | 2 PM EST They also have a yearly conference, this year its in San Antonio you would be able to visit with many of the vendors that sell scanning systems - here is the link for that https://digitalpathologyassociation.org/pathology-visions-2013. Good Luck Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 881-0763 cell (303) 682-9060 fax liz@premierlab.com Ship to address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gloria Tharp [gtharp@pcasoutheast.com] Sent: Tuesday, June 11, 2013 8:08 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Telepathology Does anyone have any recommendations for scanning equipment to have slides read off site from main lab. Thanks. Gloria Tharp, BA, HTL(ASCP) Director of Operations PCA Southeast gtharp@pcasoutheast.com 931-490-1005 800-388-1294 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SJMccabe <@t> drmc.org Tue Jun 11 13:03:36 2013 From: SJMccabe <@t> drmc.org (McCabe, Sara) Date: Tue Jun 11 13:05:38 2013 Subject: [Histonet] Her-2 Bond issues In-Reply-To: <440d6e2c-e9ec-41d0-8cc9-ca082d5dc713@EX07.drmc.org> References: <440d6e2c-e9ec-41d0-8cc9-ca082d5dc713@EX07.drmc.org> Message-ID: <02AE2390303AAB43A823930EAD6B63AE668A86AB@ex07> Robert, We had a similar problem with our Bond. It was a calibration issue. It was only noticeable with our more "delicate" antibodies. I would call for service. Sara McCabe, HT(ASCP) DuBois Regional Medical Center This email and any attached files are confidential and intended solely for the intended recipient(s). If you are not the named recipient you should not read, distribute, copy or alter this email. Any views or opinions expressed in this email are those of the author and do not represent those of the company. Warning: Although precautions have been taken to make sure no viruses are present in this email, the company cannot accept responsibility for any loss or damage that arise from the use of this email or attachments. From chesarato <@t> hotmail.com Tue Jun 11 13:48:56 2013 From: chesarato <@t> hotmail.com (Cesar Francisco Romero) Date: Tue Jun 11 13:49:02 2013 Subject: [Histonet] RE: Histonet Digest, Vol 115, Issue 11 In-Reply-To: References: Message-ID: Her 2 Antibody I do Manual IHC with Tissue Tek Slide Racks and have the same problem. I think the problem is in the holder clip. Only the top sample stains well. Cesar Romero Buenos Aires Argentina > From: histonet-request@lists.utsouthwestern.edu > Subject: Histonet Digest, Vol 115, Issue 11 > To: histonet@lists.utsouthwestern.edu > Date: Tue, 11 Jun 2013 10:00:54 -0700 > > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. RE: samples (Webb, Dorothy L) > 2. Re: Histonet Digest, Vol 115, Issue 10 (Wanda Shotsberger Gray) > 3. RE: bone marrow aspirations (Cathy) > 4. Her-2 antibody (Robert Fauck [CCDHB]) > 5. Heinz body stain on already dried blood smears. > (Jackman, Mary Ione) > 6. Telepathology (Gloria Tharp) > 7. RE: Telepathology (Elizabeth Chlipala) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Mon, 10 Jun 2013 12:24:04 -0500 > From: "Webb, Dorothy L" > Subject: [Histonet] RE: samples > To: "'sales@royalmarker.com'" > Cc: "'histonet@lists.utsouthwestern.edu'" > > Message-ID: > <65365F35C0F2EF4D846EC3CA73E49C4302758CF69446@HPEMX3.HealthPartners.int> > > Content-Type: text/plain; charset="utf-8" > > It is actually not a website but the internet communication (like a listserve) they have for PA?s. For instance, histology has one at histonet@lists.utsouthwestern.edu that is used to communicate and network online. > > Thanks again! > > Dorothy Webb > > From: sales@royalmarker.com [mailto:sales@royalmarker.com] > Sent: Monday, June 10, 2013 12:20 PM > To: Webb, Dorothy L > Subject: RE: samples > > No problem, the samples are on the way. How were you recommended for our product? Could you let us know which website you are referring to? > > Thanks Again, > Shawn Jenkins > > > -------- Original Message -------- > Subject: RE: samples > From: "Webb, Dorothy L" > > Date: Fri, June 07, 2013 1:40 pm > To: "'sales@royalmarker.com'" > > Thank you very much. Your marking inks were recommended on the website for PA?s. > > Your efficient response is greatly appreciated and we will get back to you next week with feedback! > > Dorothy Webb > > From: sales@royalmarker.com [mailto:sales@royalmarker.com] > Sent: Friday, June 07, 2013 12:30 PM > To: Webb, Dorothy L > Subject: RE: samples > > Hello Dorothy, > > We will gladly send you a sample pack of our marking dyes to try. It will ship out today via UPS to the address below that you provided. I'll be sure to have your name as well as Jesse Spates on the label as well for a contact. The set will include 10 1/4oz bottles (all of our colors). Please contact us with any comments on the product. We appreciate your feedback and hope you are happy with our products. Have a great weekend and look forward to hearing from you. > > Best Regards, > Shawn Jenkins > Royal Marker > (P) 954-462-0264 > (F) 954-374-7290 > > > -------- Original Message -------- > Subject: samples > From: "Webb, Dorothy L" > > Date: Fri, June 07, 2013 11:59 am > To: "'sales@royalmarker.com'" > > Cc: "Spates, Jesse D" > > Our PA?s are looking to try other inks than what we are currently using so we are wondering if we could please get some ink samples. > > Our hospital is Regions Hospital > 640 Jackson St. > St. Paul, MN. 55101 > > I am the technical specialist of histology, Dorothy Webb and the lead PA is Jesse Spates @ 651-254-4913. > > Thank you, > > Dorothy Webb > Regions Histology Technical Specialist > 651-254-2962 > > > ________________________________ > This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. > > If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 > > ------------------------------ > > Message: 2 > Date: Mon, 10 Jun 2013 18:00:12 -0400 > From: Wanda Shotsberger Gray > Subject: [Histonet] Re: Histonet Digest, Vol 115, Issue 10 > To: histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; charset=utf-8 > > Hi Megan, > Welcome to the anatomic side! I just googled "history of tissue dyes" and a wealth of information popped up including this PDF: www.immunologie-labor.com/cellmarker_files/IET_reagents_07.pdf > all about the discovery of hematoxylin and aniline dyes. The time period you mentioned was the time of these discoveries, so searching for history of hematoxylin or eosin should also get you good hits. Good luck! > Wanda Shotsberger > HT/HTL (ASCP) > > ------------------------------ > > Message: 3 > Date: Mon, 10 Jun 2013 17:03:14 -0700 > From: "Cathy" > Subject: RE: [Histonet] bone marrow aspirations > To: "'Shea's'" , > > Message-ID: <001b01ce6637$11eb70c0$35c25240$@007group.com> > Content-Type: text/plain; charset="us-ascii" > > We are using a similar method at our site. The aspirate is placed into EDTA > and then into formalin to fix. We pour the aspirate through a biopsy bag, > open the bag and scrape the spicules together, transfer them to another > biopsy bag and process as usual. I have received several compliments on the > quality of the slides. > > Cathy > Kelowna, B.C. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shea's > Sent: Saturday, June 1, 2013 4:26 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] bone marrow aspirations > > I am also interested in how others are processing bone marrow aspirates. > Currently, we let the aspirate clot on a watch glass for about 20 minutes, > gentle slide it onto filter paper, roll it around to get rid of the excess > fluid, place the clot between sponges, and process as usual. > However, I am interested in knowing if any one out there is using a > technique that we use to use at another hospital 33 years ago. > If I remember correctly, the pathologist or oncologist would collect the > aspirate using a heparin syringe. Some how, the aspirate smears for > Hematology were made on 22x22 cover slips instead of slides. Then the EDTA > tube was then given to Histology. I can't remember if we added formalin or > B5 fixative to it at that time, but it was filtered into an obex tea bag > type filter paper (The blood never clotted and ran through the filter paper, > leaving only bone marrow spicules in the paper). We would fold the paper, > place it in the cassette, and process as usual. The pathologist preferred > this method because he wouldn't have to look at levels of clot to find > spicules. Is there anyone out there using this method? > Jan > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 4 > Date: Tue, 11 Jun 2013 17:04:35 +1200 > From: "Robert Fauck [CCDHB]" > Subject: [Histonet] Her-2 antibody > To: histonet@lists.utsouthwestern.edu > Message-ID: > <9C92DFB6EA9983408AA9785182E9FC966804DE@WN0NTEML11.AD.CCDHB.HEALTH.NZ> > Content-Type: text/plain; charset=us-ascii > > Hi All > > We here in wellington Hospital New Zealand having some problem with the > Her-2 ( Lieca, NCL-L-CBE-356 ) > > Top half of the slide ( control or Test tissue) are strongly positive > but if we put the control tissue on the bottom half it is week to > negative! > > Does any one has the same problems with your Her-2, it is done on a > Leica Bond (Max) III. > > Much appreciated for any suggestions, also from Leica Technical > specialists. > > Regards, > Robert Fauck > Wellington hospital, NZ > > > This email or attachment(s) may contain confidential or legally privileged information intended for the sole use of the addressee(s). Any use, redistribution, disclosure, or reproduction of this message, except as intended, is prohibited. If you received this email in error, please notify the sender and remove all copies of the message, including any attachments. Any views or opinions expressed in this email (unless otherwise stated) may not represent those of Capital & Coast District Health Board. > > http://www.ccdhb.org.nz > > (1C_S1) > > > > ------------------------------ > > Message: 5 > Date: Tue, 11 Jun 2013 13:34:09 +0000 > From: "Jackman, Mary Ione" > Subject: [Histonet] Heinz body stain on already dried blood smears. > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > Good Morning, > Does anyone have a protocol they like for demonstrating Heinz bodies on already dried blood smears? There is no blood in tubes available any longer. I saw a citation for using Wrights Giemsa, but is there one that is more specific that anyone uses and is willing to share? > Thanks. > Ione Jackman > > > > > ------------------------------ > > Message: 6 > Date: Tue, 11 Jun 2013 09:08:40 -0500 > From: "Gloria Tharp" > Subject: [Histonet] Telepathology > To: > Message-ID: <006701ce66ad$2c971fc0$85c55f40$@com> > Content-Type: text/plain; charset="us-ascii" > > Does anyone have any recommendations for scanning equipment to have slides > read off site from main lab. Thanks. > > > > > > Gloria Tharp, BA, HTL(ASCP) > > Director of Operations > > PCA Southeast > > gtharp@pcasoutheast.com > > 931-490-1005 > > 800-388-1294 > > > > > > ------------------------------ > > Message: 7 > Date: Tue, 11 Jun 2013 08:28:20 -0600 > From: Elizabeth Chlipala > Subject: RE: [Histonet] Telepathology > To: Gloria Tharp , > "Histonet@lists.utsouthwestern.edu" > > Message-ID: > <14E2C6176416974295479C64A11CB9AE016411AB4898@SBS2K8.premierlab.local> > Content-Type: text/plain; charset="us-ascii" > > Gloria > > There are many systems out there that perform scanning, your choice is dependent upon how you are going to be utilizing the instrument, how many slides per day that you will need to scan, if you will be utilizing any image analysis algorithms, etc. > > The DPA has lots information on its website - https://digitalpathologyassociation.org/ > > They are also hosting a free webinar on July 10th (this is the 1st of 4) to sign up just go to this link https://digitalpathologyassociation.org/2013-dpa-webinar-series > The webinar is titled Real-World Examples of Practical Consultative/Collaborative Digital Pathology Programs > Wednesday, July 10 | 2 PM EST > > They also have a yearly conference, this year its in San Antonio you would be able to visit with many of the vendors that sell scanning systems - here is the link for that > https://digitalpathologyassociation.org/pathology-visions-2013. > > Good Luck > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > Premier Laboratory, LLC > PO Box 18592 > Boulder, CO 80308 > (303) 682-3949 office > (303) 881-0763 cell > (303) 682-9060 fax > liz@premierlab.com > > Ship to address: > > Premier Laboratory, LLC > 1567 Skyway Drive, Unit E > Longmont, CO 80504 > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gloria Tharp [gtharp@pcasoutheast.com] > Sent: Tuesday, June 11, 2013 8:08 AM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] Telepathology > > Does anyone have any recommendations for scanning equipment to have slides > read off site from main lab. Thanks. > > > > > > Gloria Tharp, BA, HTL(ASCP) > > Director of Operations > > PCA Southeast > > gtharp@pcasoutheast.com > > 931-490-1005 > > 800-388-1294 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 115, Issue 11 > ***************************************** From Kristopher.Kalleberg <@t> unilever.com Tue Jun 11 14:05:03 2013 From: Kristopher.Kalleberg <@t> unilever.com (Kalleberg, Kristopher) Date: Tue Jun 11 14:05:15 2013 Subject: [Histonet] Biocare Medical Mach 4 Message-ID: Hello All, Does anyone have any knowledge of the Biocare Medical Mach 4 AP detection system. How does this system exactly work. Does it use a dextran backbone with 2ndary antibodies attached like other systems and then the polymer binds to that? Their datasheets simply state that it uses a specific probe and then the polymer binds to that. I am very pleased with the results I have achieved with this system but am a little confused on the technique of labeling. Any knowledge on this topic would be greatly appreciated. And as always, thanks in advance. Kris From tkngflght <@t> yahoo.com Tue Jun 11 14:49:02 2013 From: tkngflght <@t> yahoo.com (Cheryl) Date: Tue Jun 11 14:49:07 2013 Subject: [Histonet] Leica filter available - CV5000 Message-ID: <1370980142.9333.YahooMailClassic@web161902.mail.bf1.yahoo.com> Found an unused Leice Coverslip unit CV5000 filter in a cupboard---anyone need it? ? Thank you! Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab by helping one GREAT?Tech at a time.? 281.852.9457?Office 800.756.3309?Phone & Fax? admin@fullstaff.org Sign up for the FREE?newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please?include your name and specialty in the body of the email. From marktarango <@t> gmail.com Tue Jun 11 16:59:03 2013 From: marktarango <@t> gmail.com (Mark Tarango) Date: Tue Jun 11 16:59:07 2013 Subject: [Histonet] Biocare Medical Mach 4 In-Reply-To: References: Message-ID: Hi Kris, The polymer backbone for this detection kit is conjugated with anti-rabbit antibodies only (AP Polymer reagent). The specific probe is a rabbit anti-mouse antibody in the AP probe reagent. That is why you only need to apply the AP probe when your primary antibody is from mouse but not when it's rabbit. Mark On Tue, Jun 11, 2013 at 12:05 PM, Kalleberg, Kristopher < Kristopher.Kalleberg@unilever.com> wrote: > Hello All, > > Does anyone have any knowledge of the Biocare Medical Mach 4 AP detection > system. How does this system exactly work. Does it use a dextran backbone > with 2ndary antibodies attached like other systems and then the polymer > binds to that? Their datasheets simply state that it uses a specific probe > and then the polymer binds to that. I am very pleased with the results I > have achieved with this system but am a little confused on the technique of > labeling. Any knowledge on this topic would be greatly appreciated. And > as always, thanks in advance. > > Kris > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From ibernard <@t> uab.edu Wed Jun 12 06:57:52 2013 From: ibernard <@t> uab.edu (Ian R Bernard) Date: Wed Jun 12 06:58:00 2013 Subject: [Histonet] Telepathology In-Reply-To: <006701ce66ad$2c971fc0$85c55f40$@com> References: <006701ce66ad$2c971fc0$85c55f40$@com> Message-ID: We are a test site for the Aperio System. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gloria Tharp Sent: Tuesday, June 11, 2013 9:09 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Telepathology Does anyone have any recommendations for scanning equipment to have slides read off site from main lab. Thanks. Gloria Tharp, BA, HTL(ASCP) Director of Operations PCA Southeast gtharp@pcasoutheast.com 931-490-1005 800-388-1294 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SHUNTER <@t> beaumont.edu Wed Jun 12 09:41:41 2013 From: SHUNTER <@t> beaumont.edu (Sue Hunter) Date: Wed Jun 12 09:41:46 2013 Subject: [Histonet] RE: Her-2 antibody In-Reply-To: <9C92DFB6EA9983408AA9785182E9FC966804DE@WN0NTEML11.AD.CCDHB.HEALTH.NZ> References: <9C92DFB6EA9983408AA9785182E9FC966804DE@WN0NTEML11.AD.CCDHB.HEALTH.NZ> Message-ID: We have a Bond Max and run mainly ER/PR and ISH stains on that. After Leica loaded in a new version of software in October we started having problems like that too. Seems you have to have all 10 spots on the tray filled with a slide - doesn't have to be programed, just a slide and cover tile for the weight. We also were doing a workaround with a Vantage label under the Leica label and found out that the two label thickness interferes with the movement of the cover tile (sometimes...but not always), which was affecting our staining. Even a regular slide label under the Leica label will cause a problem. We finally have gone to entering a case, printing the labels but not putting them on the slides, cutting the sections and then putting the labels on the slide for positive patient identification. Not ideal, and I worry a lot about case mix ups, but that is what is working to get consistent staining here. As they say, The Enemy of Good is Better. Wish Leica had not made it better! It was working very nicely before. Sue Sue Hunter, Supervisor Advanced Diagnostics Beaumont Health System Royal Oak MI 248-898-5146 shunter@beaumont.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Fauck [CCDHB] Sent: Tuesday, June 11, 2013 1:05 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Her-2 antibody Hi All We here in wellington Hospital New Zealand having some problem with the Her-2 ( Lieca, NCL-L-CBE-356 ) Top half of the slide ( control or Test tissue) are strongly positive but if we put the control tissue on the bottom half it is week to negative! Does any one has the same problems with your Her-2, it is done on a Leica Bond (Max) III. Much appreciated for any suggestions, also from Leica Technical specialists. Regards, Robert Fauck Wellington hospital, NZ This email or attachment(s) may contain confidential or legally privileged information intended for the sole use of the addressee(s). Any use, redistribution, disclosure, or reproduction of this message, except as intended, is prohibited. If you received this email in error, please notify the sender and remove all copies of the message, including any attachments. Any views or opinions expressed in this email (unless otherwise stated) may not represent those of Capital & Coast District Health Board. http://www.ccdhb.org.nz (1C_S1) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LMurphy2 <@t> aultman.com Wed Jun 12 09:55:28 2013 From: LMurphy2 <@t> aultman.com (Leann M. Murphy) Date: Wed Jun 12 09:55:35 2013 Subject: [Histonet] Waterbaths Message-ID: <3CA7BA3FBC705C45870DDB88E634192040643B@pa01exc001.ahf2000.aultman.com> What are the best water bath to use? I am using the Boekel water baths and they always seem to become rusty and we have to purchase new ones. Any suggestions? LeAnn Murphy Aultman Hospital Canton, Ohio From Ronald.Houston <@t> nationwidechildrens.org Wed Jun 12 09:59:09 2013 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Wed Jun 12 09:59:37 2013 Subject: [Histonet] RE: Her-2 antibody In-Reply-To: References: <9C92DFB6EA9983408AA9785182E9FC966804DE@WN0NTEML11.AD.CCDHB.HEALTH.NZ> Message-ID: Never had any such problems with either the Max or the Bond IIIs in the 6+ years we have had them(no matter how many labels are on the slide - we have had three labels on a slide with no problems at all), unless of course your tissue extends to the rim of the covertile - then what would you expect? You certainly do not need to have all 10 spaces filled on the tray. Suggest you contact Leica service as it seems as though your machine needs calibrated and realigned Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org "One person with passion is better than forty people merely interested." ~ E.M. Forster -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sue Hunter Sent: Wednesday, June 12, 2013 10:42 AM To: Robert Fauck [CCDHB]; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Her-2 antibody We have a Bond Max and run mainly ER/PR and ISH stains on that. After Leica loaded in a new version of software in October we started having problems like that too. Seems you have to have all 10 spots on the tray filled with a slide - doesn't have to be programed, just a slide and cover tile for the weight. We also were doing a workaround with a Vantage label under the Leica label and found out that the two label thickness interferes with the movement of the cover tile (sometimes...but not always), which was affecting our staining. Even a regular slide label under the Leica label will cause a problem. We finally have gone to entering a case, printing the labels but not putting them on the slides, cutting the sections and then putting the labels on the slide for positive patient identification. Not ideal, and I worry a lot about case mix ups, but that is what is working to get consistent staining here. As they say, The Enemy of Good is Better. Wish Leica had not made it better! It was working very nicely before. Sue Sue Hunter, Supervisor Advanced Diagnostics Beaumont Health System Royal Oak MI 248-898-5146 shunter@beaumont.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Fauck [CCDHB] Sent: Tuesday, June 11, 2013 1:05 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Her-2 antibody Hi All We here in wellington Hospital New Zealand having some problem with the Her-2 ( Lieca, NCL-L-CBE-356 ) Top half of the slide ( control or Test tissue) are strongly positive but if we put the control tissue on the bottom half it is week to negative! Does any one has the same problems with your Her-2, it is done on a Leica Bond (Max) III. Much appreciated for any suggestions, also from Leica Technical specialists. Regards, Robert Fauck Wellington hospital, NZ This email or attachment(s) may contain confidential or legally privileged information intended for the sole use of the addressee(s). Any use, redistribution, disclosure, or reproduction of this message, except as intended, is prohibited. If you received this email in error, please notify the sender and remove all copies of the message, including any attachments. Any views or opinions expressed in this email (unless otherwise stated) may not represent those of Capital & Coast District Health Board. http://www.ccdhb.org.nz (1C_S1) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cmiller <@t> physlab.com Wed Jun 12 10:12:47 2013 From: cmiller <@t> physlab.com (Cheri Miller) Date: Wed Jun 12 10:12:53 2013 Subject: [Histonet] RE: Waterbaths In-Reply-To: <3CA7BA3FBC705C45870DDB88E634192040643B@pa01exc001.ahf2000.aultman.com> References: <3CA7BA3FBC705C45870DDB88E634192040643B@pa01exc001.ahf2000.aultman.com> Message-ID: I agree with you. Ours seem to short out as well. I now only purchase the baths that have a separate Pyrex glass insert. They also provide more of an "edge" space to lay out your ribbons when cutting multiple levels and ribbons. Cheri "March 10, 2013 is Histotechnology Professionals Day" Cheryl A. Miller HT(ASCP)cm Histology Supervisor, Hygiene Officer Physicians Laboratory Services 4840 F Street Omaha, NE. 68127-0999 402 731 4145 ext. 554 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Leann M. Murphy Sent: Wednesday, June 12, 2013 9:55 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Waterbaths What are the best water bath to use? I am using the Boekel water baths and they always seem to become rusty and we have to purchase new ones. Any suggestions? LeAnn Murphy Aultman Hospital Canton, Ohio _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From SHUNTER <@t> beaumont.edu Wed Jun 12 10:20:52 2013 From: SHUNTER <@t> beaumont.edu (Sue Hunter) Date: Wed Jun 12 10:21:00 2013 Subject: [Histonet] RE: Her-2 antibody In-Reply-To: References: <9C92DFB6EA9983408AA9785182E9FC966804DE@WN0NTEML11.AD.CCDHB.HEALTH.NZ> Message-ID: We have contacted Leica several times, had the machine calibrated/aligned many times, and that is what we have been told. It seems to hold true as with only one label we have no problems. Also, after the upgrade, we saw the problem a lot when we only ran one or two slides on a tray. The magic number seems to be 4 slides to get acceptable staining, but Leica suggests we load it up. We had never had a problem until the upgrade in software. Sue -----Original Message----- From: Houston, Ronald [mailto:Ronald.Houston@nationwidechildrens.org] Sent: Wednesday, June 12, 2013 10:59 AM To: Sue Hunter; Robert Fauck [CCDHB]; histonet@lists.utsouthwestern.edu Subject: RE: Her-2 antibody Never had any such problems with either the Max or the Bond IIIs in the 6+ years we have had them(no matter how many labels are on the slide - we have had three labels on a slide with no problems at all), unless of course your tissue extends to the rim of the covertile - then what would you expect? You certainly do not need to have all 10 spaces filled on the tray. Suggest you contact Leica service as it seems as though your machine needs calibrated and realigned Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org "One person with passion is better than forty people merely interested." ~ E.M. Forster -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sue Hunter Sent: Wednesday, June 12, 2013 10:42 AM To: Robert Fauck [CCDHB]; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Her-2 antibody We have a Bond Max and run mainly ER/PR and ISH stains on that. After Leica loaded in a new version of software in October we started having problems like that too. Seems you have to have all 10 spots on the tray filled with a slide - doesn't have to be programed, just a slide and cover tile for the weight. We also were doing a workaround with a Vantage label under the Leica label and found out that the two label thickness interferes with the movement of the cover tile (sometimes...but not always), which was affecting our staining. Even a regular slide label under the Leica label will cause a problem. We finally have gone to entering a case, printing the labels but not putting them on the slides, cutting the sections and then putting the labels on the slide for positive patient identification. Not ideal, and I worry a lot about case mix ups, but that is what is working to get consistent staining here. As they say, The Enemy of Good is Better. Wish Leica had not made it better! It was working very nicely before. Sue Sue Hunter, Supervisor Advanced Diagnostics Beaumont Health System Royal Oak MI 248-898-5146 shunter@beaumont.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Fauck [CCDHB] Sent: Tuesday, June 11, 2013 1:05 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Her-2 antibody Hi All We here in wellington Hospital New Zealand having some problem with the Her-2 ( Lieca, NCL-L-CBE-356 ) Top half of the slide ( control or Test tissue) are strongly positive but if we put the control tissue on the bottom half it is week to negative! Does any one has the same problems with your Her-2, it is done on a Leica Bond (Max) III. Much appreciated for any suggestions, also from Leica Technical specialists. Regards, Robert Fauck Wellington hospital, NZ This email or attachment(s) may contain confidential or legally privileged information intended for the sole use of the addressee(s). Any use, redistribution, disclosure, or reproduction of this message, except as intended, is prohibited. If you received this email in error, please notify the sender and remove all copies of the message, including any attachments. Any views or opinions expressed in this email (unless otherwise stated) may not represent those of Capital & Coast District Health Board. http://www.ccdhb.org.nz (1C_S1) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Colleen_Herring <@t> bshsi.org Wed Jun 12 10:48:33 2013 From: Colleen_Herring <@t> bshsi.org (Herring, Colleen) Date: Wed Jun 12 10:48:50 2013 Subject: [Histonet] Control Storage Message-ID: <3AE5857C7EC73F4E983F39C97DF811C09C23@EDC-EXMB2-01.ads.bshsi.com> Does anyone have any ideas or suggestions about the storage of blocks and precut slides for immunos? ________________________________________________________________________________________________________ The information in this communication is intended to be confidential to the Individual(s) and/or Entity to whom it is addressed. It may contain information of a Privileged and/or Confidential nature, which is subject to Federal and/or State privacy regulations. In the event that you are not the intended recipient or the agent of the intended recipient, do not copy or use the information contained within this communication, or allow it to be read, copied or utilized in any manner, by any other person(s). Should this communication be received in error, please notify the sender immediately either by response e-mail or by phone, and permanently delete the original e-mail, attachment(s), and any copies. ________________________________________________________________________________________________________ From liz <@t> premierlab.com Wed Jun 12 10:55:45 2013 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Wed Jun 12 10:56:34 2013 Subject: [Histonet] RE: Control Storage In-Reply-To: <3AE5857C7EC73F4E983F39C97DF811C09C23@EDC-EXMB2-01.ads.bshsi.com> References: <3AE5857C7EC73F4E983F39C97DF811C09C23@EDC-EXMB2-01.ads.bshsi.com> Message-ID: <14E2C6176416974295479C64A11CB9AE016411AB48B0@SBS2K8.premierlab.local> We store our blocks at room temp, our IHC slides are stored in the fridge. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 881-0763 cell (303) 682-9060 fax liz@premierlab.com Ship to address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Herring, Colleen [Colleen_Herring@bshsi.org] Sent: Wednesday, June 12, 2013 9:48 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Control Storage Does anyone have any ideas or suggestions about the storage of blocks and precut slides for immunos? ________________________________________________________________________________________________________ The information in this communication is intended to be confidential to the Individual(s) and/or Entity to whom it is addressed. It may contain information of a Privileged and/or Confidential nature, which is subject to Federal and/or State privacy regulations. In the event that you are not the intended recipient or the agent of the intended recipient, do not copy or use the information contained within this communication, or allow it to be read, copied or utilized in any manner, by any other person(s). Should this communication be received in error, please notify the sender immediately either by response e-mail or by phone, and permanently delete the original e-mail, attachment(s), and any copies. ________________________________________________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hfedor <@t> jhmi.edu Wed Jun 12 11:01:42 2013 From: hfedor <@t> jhmi.edu (Helen Fedor) Date: Wed Jun 12 11:01:59 2013 Subject: [Histonet] RE: Control Storage In-Reply-To: <14E2C6176416974295479C64A11CB9AE016411AB48B0@SBS2K8.premierlab.local> References: <3AE5857C7EC73F4E983F39C97DF811C09C23@EDC-EXMB2-01.ads.bshsi.com> <14E2C6176416974295479C64A11CB9AE016411AB48B0@SBS2K8.premierlab.local> Message-ID: Hello, We are storing our unstained slides at -20 in Ziploc bags. Our TMA blocks are being stored at 4 degrees. Helen L. Fedor Oncology Tissue Services, Manager Johns Hopkins University 600 N. Wolfe St,?| Marburg Room 406 Baltimore, MD?| 21287-7065 410.614.1660 http://tmalab.jhmi.edu/ http://prostatebiorepository.org/ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala Sent: Wednesday, June 12, 2013 11:56 AM To: Herring, Colleen; Histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Control Storage We store our blocks at room temp, our IHC slides are stored in the fridge. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 881-0763 cell (303) 682-9060 fax liz@premierlab.com Ship to address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Herring, Colleen [Colleen_Herring@bshsi.org] Sent: Wednesday, June 12, 2013 9:48 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Control Storage Does anyone have any ideas or suggestions about the storage of blocks and precut slides for immunos? ________________________________________________________________________________________________________ The information in this communication is intended to be confidential to the Individual(s) and/or Entity to whom it is addressed. It may contain information of a Privileged and/or Confidential nature, which is subject to Federal and/or State privacy regulations. In the event that you are not the intended recipient or the agent of the intended recipient, do not copy or use the information contained within this communication, or allow it to be read, copied or utilized in any manner, by any other person(s). Should this communication be received in error, please notify the sender immediately either by response e-mail or by phone, and permanently delete the original e-mail, attachment(s), and any copies. ________________________________________________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vvelez <@t> usgs.gov Wed Jun 12 11:49:40 2013 From: vvelez <@t> usgs.gov (Velez, Vanessa) Date: Wed Jun 12 11:49:58 2013 Subject: [Histonet] Shandon Excelsior Tissue Processor Message-ID: Does any one having problems with the Excelsior Tissue Processor power board repeatedly after power outage? This has happened to me three times in the last five years even though it has backup batteries. From mroark <@t> sfmc.net Wed Jun 12 13:18:51 2013 From: mroark <@t> sfmc.net (Matthew Roark) Date: Wed Jun 12 13:19:19 2013 Subject: [Histonet] Tissue Processors Message-ID: <000f01ce6799$4a64f2b0$df2ed810$@net> So in the next couple of months we are getting ready to demo Thermo's STP 420ES and Leica's ASP6025 tissue processor . Looking for the good, bad, and ugly about them. So if you have one or have had a demo please share what you've found. Thanks!! Matthew Roark- HT/HTL(ASCP)CM Histology Specialist Saint Francis Medical Center 211 Saint Francis Drive Cape Girardeau, MO 63703 573-331-5267 mroark@sfmc.net http://www.sfmc.net From anolan <@t> prometheushealthcare.com Wed Jun 12 14:19:34 2013 From: anolan <@t> prometheushealthcare.com (Anna Nolan) Date: Wed Jun 12 14:19:41 2013 Subject: [Histonet] Histology Openings Message-ID: <00c801ce67a1$c696cbd0$53c46370$@prometheushealthcare.com> Hi my name is Anna and I work with Prometheus Healthcare doing laboratory recruitment. Currently I'm looking to fill several histology positions. Please call or email me if any of these positions are of interest to you. Portland, ME -Histology Technologist (3AM-11AM) NYC -Grossing Specialist (3rd shift) -Histology Tech (1st shift) Suffern, NY -IHC Histology Tech (1st shift) -Cutting embedding, staining, and coverslipping. IHC Experience a plus. (7AM-4PM) Anna Nolan Recruiter Prometheus Healthcare Office 301-693-9057 Cell 301-693-8908 Fax 301-368-2478 anolan @prometheushealthcare.com http://www.linkedin.com/pub/annelise-nolan/55/ba0/ab6 www.prometheushealthcare.com From lizronan <@t> umich.edu Wed Jun 12 15:59:52 2013 From: lizronan <@t> umich.edu (Elizabeth Ronan) Date: Wed Jun 12 15:59:59 2013 Subject: [Histonet] Paraffin processing native sheep ACL Message-ID: <51B8E148.3020102@umich.edu> Hello, I need to paraffin process native sheep anterior cruciate ligament (ACL) that has been fixed in 10% neutral buffered formalin for 7 days. I was wondering if anyone with more expertise on this subject could guide me with the best lengths of times in the alcohols, xylenes, and paraffin to fix ACL. I tried a 12 hour program but the sections crumbled in the middle and it appeared that the paraffin had not fully perfused the ligament. I have access to the following program, and can alter the lengths of the steps for as long as desired: Program: 70% 80% 95% 95% 100% 100% Xylene Xylene Paraffin Paraffin Paraffin Any advice is much appreciated. Thanks for your time, Liz From tony.henwood <@t> health.nsw.gov.au Wed Jun 12 18:40:22 2013 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Wed Jun 12 18:40:44 2013 Subject: [Histonet] Shandon Excelsior Tissue Processor In-Reply-To: References: Message-ID: <6D6BD1DE8A5571489398B392A38A71579D271C66@xmdb04.nch.kids> Vanessa, We installed an Eaton 9130 UPS unit with a SSFIO Surge Protector. Cost under $1,500 Australian. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Velez, Vanessa Sent: Thursday, 13 June 2013 2:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Shandon Excelsior Tissue Processor Does any one having problems with the Excelsior Tissue Processor power board repeatedly after power outage? This has happened to me three times in the last five years even though it has backup batteries. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From PAMarcum <@t> uams.edu Thu Jun 13 06:05:48 2013 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Thu Jun 13 06:05:53 2013 Subject: [Histonet] Shandon Excelsior Tissue Processor In-Reply-To: <6D6BD1DE8A5571489398B392A38A71579D271C66@xmdb04.nch.kids> References: , <6D6BD1DE8A5571489398B392A38A71579D271C66@xmdb04.nch.kids> Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA32CA17004C@Mail2Node2.ad.uams.edu> Since we have some power issues here and 4 Excelsiors (no problems with them on this issue) we have UPS units on all of our computerized equipment. We did this due to issues in other areas of the lab and decided it was safer than taking a chance and being down. We bought Powervar and I think the suggested units were around $2,000 to $3,000 each with one unit serving two processors. Other sites in Little Rock who have some of the same power issues have had problems. Pam Marcum UAMS ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Tony Henwood (SCHN) [tony.henwood@health.nsw.gov.au] Sent: Wednesday, June 12, 2013 6:40 PM To: 'Velez, Vanessa'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Shandon Excelsior Tissue Processor Vanessa, We installed an Eaton 9130 UPS unit with a SSFIO Surge Protector. Cost under $1,500 Australian. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Velez, Vanessa Sent: Thursday, 13 June 2013 2:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Shandon Excelsior Tissue Processor Does any one having problems with the Excelsior Tissue Processor power board repeatedly after power outage? This has happened to me three times in the last five years even though it has backup batteries. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From carnold <@t> ourlab.net Thu Jun 13 08:15:23 2013 From: carnold <@t> ourlab.net (Casey Arnold) Date: Thu Jun 13 08:15:29 2013 Subject: [Histonet] Sakura Tissue-Tek Prisma Message-ID: We have recently started using a Sakura Tissue-tek Prisma that we inherited from our sister lab. While running some validation testing I have noticed that the staining is very inconsistent. The tissue will stain from light to dark within the same rack and sometimes within the same cut (slide). I have reset the mix, speed, etc, to factory settings. I have tried increasing my hematoxylin, and bluing times. I have changed water filters, decreased water rinse times, tried different types of tissue, all to no avail. Has anyone had this problem or have any suggestions on how to correct it? I have tried everything the Sakura tech line has recommended and am still at a loss. -- NOTICE: This electronic mail message and any files transmitted with it are intended exclusively for the individual or entity to which it is addressed. The message, together with any attachment, may contain confidential and/or privileged information. Any unauthorized review, use, printing, saving, copying, disclosure or distribution is strictly prohibited. If you have received this message in error, please immediately advise the sender by reply email and delete all copies. Thank you *Casey Arnold * *Histology Supervisor** OURLabs / OPKO Diagnostics, LLC* 1450 Elm Hill Pike Nashville, TN 37210 Direct: 615-345-4582 Office: 615-874-0410 Fax: 615-345-4595 From akbitting <@t> geisinger.edu Thu Jun 13 09:21:16 2013 From: akbitting <@t> geisinger.edu (Bitting, Angela K.) Date: Thu Jun 13 09:21:28 2013 Subject: [Histonet] RE: Control Storage In-Reply-To: <14E2C6176416974295479C64A11CB9AE016411AB48B0@SBS2K8.premierlab.local> References: <3AE5857C7EC73F4E983F39C97DF811C09C23@EDC-EXMB2-01.ads.bshsi.com> <14E2C6176416974295479C64A11CB9AE016411AB48B0@SBS2K8.premierlab.local> Message-ID: <77F52EFAB8B1694B885E277C48FCD0F647C7298D@GHSEXMBX4W8K1V.geisinger.edu> Something to remember is that humidity is the enemy too. If you are storing your slides in a refrigerator make sure that the humidity is well controlled. We found out the hard way. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala Sent: Wednesday, June 12, 2013 11:56 AM To: Herring, Colleen; Histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Control Storage We store our blocks at room temp, our IHC slides are stored in the fridge. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 881-0763 cell (303) 682-9060 fax liz@premierlab.com Ship to address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Herring, Colleen [Colleen_Herring@bshsi.org] Sent: Wednesday, June 12, 2013 9:48 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Control Storage Does anyone have any ideas or suggestions about the storage of blocks and precut slides for immunos? ________________________________________________________________________________________________________ The information in this communication is intended to be confidential to the Individual(s) and/or Entity to whom it is addressed. It may contain information of a Privileged and/or Confidential nature, which is subject to Federal and/or State privacy regulations. In the event that you are not the intended recipient or the agent of the intended recipient, do not copy or use the information contained within this communication, or allow it to be read, copied or utilized in any manner, by any other person(s). Should this communication be received in error, please notify the sender immediately either by response e-mail or by phone, and permanently delete the original e-mail, attachment(s), and any copies. ________________________________________________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. ------------------------------------------------------------------------- This message was secured by ZixCorp(R). From amber.mckenzie <@t> gastrodocs.net Thu Jun 13 09:22:10 2013 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Thu Jun 13 09:22:14 2013 Subject: [Histonet] Old books In-Reply-To: References: <5BD80891-C417-4913-8A0D-2F40AA47F8DB@gmail.com> Message-ID: <5A33C952BB67F4468AF1F36D739212BCBD6ADF63@JERRY.Gia.com> I'm cleaning house :) Anyone interested in buying these old books? 1. A color Atlas of Histology by Dennis Strete, ISBN 0-06-501084-1 2. Human Histology, 2nd edition, by Alan Stevens and James Lowe, ISBN 0-7234-2485-3 3. Immunolgy, 4th edition, by Roitt, Brostoff, Male ISBN 0-7234-2178-1 4. Atlas of Histology with Funcitonal correlations, by Victor P. Erodschenko ISBN 0-583-2818-9 5. Histotechnology, A Self Instructional Text, 2nd edition, by Freida Carson (I'm interested in purchasing the 3rd edition). From megan-flynn <@t> northwestern.edu Thu Jun 13 10:11:12 2013 From: megan-flynn <@t> northwestern.edu (Megan Elizabeth Flynn) Date: Thu Jun 13 10:11:17 2013 Subject: [Histonet] cutting arterial grafts Message-ID: <5748287483CD8A48BC4A27D05908D4E215E72FFC@CHCSPMBX3.ads.northwestern.edu> Hi all, I am in a research lab and I am cutting rat arterial grafts made with ePTFE. I'm cutting at 30 degrees at 5 microns. The lower edge of the graft seems to be lifting up from the adventitia and folding over. I have tried playing with the temperature, but none of it seems to make a difference. Can anyone help? Megan From idimitro <@t> mun.ca Thu Jun 13 10:55:23 2013 From: idimitro <@t> mun.ca (idimitro@mun.ca) Date: Thu Jun 13 10:55:31 2013 Subject: [Histonet] Electron Microscopy protocols In-Reply-To: References: Message-ID: <14C3108E8B98EF43B3EDAE58336700341FA60F@exchange.med.mun.ca> Hi Joel, Lipids are fixed only by OsO4, so you need to use it as a post-fixative. As a fixative in our lab we use Karnovsky fixative, then we use osmium tetroxide as a post-fixative/fixative for the lipids for 15 min. Then we wash in Na-cacodylate buffer for 5 min, and then dehydrate, starting with two changes of 70% ethanol ( for 10min.) and then we go to two changes of 95% and then absolute ethanol, followed by two changes of abs. acetone, then 50:50 acetone:resin and 2 changes of resin, then embedding and polymerization at the right temperature. If your lipids are fixed properly they will be round droplets with characteristic appearance. OsO4 will blacken the tissue. We always have excellent results with this protocol. Hope that was helpful, Iliana dimitrova, Memorial university of Newfoundland, Canada -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joel Haas Sent: June-05-13 3:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Electron Microscopy protocols Hello all, I am working on developing an electron microscopy protocol for looking at lipid droplets in cultured cells. We have used thin section TEM previously and found that the morphology of the droplets is often deformed (should be spherical, shows up as egg shaped or lumpy). If possible we would like to avoid these types of preservation artifacts. Does anyone have suggestions for adapting the protocol to better preserve these types of structures--a triglyceride lipid core surrounded by a phospholipid monolayer? Thanks in advance, Joel _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic communication is governed by the terms and conditions at http://www.mun.ca/cc/policies/electronic_communications_disclaimer_2012.php From Andrea.X.Dejager <@t> kp.org Thu Jun 13 12:25:39 2013 From: Andrea.X.Dejager <@t> kp.org (Andrea.X.Dejager@kp.org) Date: Thu Jun 13 12:26:14 2013 Subject: [Histonet] Re: Histonet Digest, Vol 115, Issue 13 In-Reply-To: <201306131704.r5DH4Tfp031015@masdcsmrp112.kp.org> References: <201306131704.r5DH4Tfp031015@masdcsmrp112.kp.org> Message-ID: This message is for Casey Arnold, we never had any problems with our stainer/coverslipper, I will send you our procedure to try out. Do you dry slides on the stainer or elsewhere? Andrea De Jager, H.T. ASCP Histology Manager, Regional Reference Lab Kaiser Permanente - Colorado Phone: 303-404-4152 Fax: 303-404-4161 email: ANDREA.X.DEJAGER@KP.org NOTICE TO RECIPIENT: If you are not the intended recipient of this e-mail, you are prohibited from sharing, copying, or otherwise using or disclosing its contents. If you have received this e-mail in error, please notify the sender immediately by reply e-mail and permanently delete this e-mail and any attachments without reading, forwarding or saving them. Thank you. From: histonet-request@lists.utsouthwestern.edu To: histonet@lists.utsouthwestern.edu Date: 06/13/2013 11:04 AM Subject: Histonet Digest, Vol 115, Issue 13 Sent by: histonet-bounces@lists.utsouthwestern.edu Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Tissue Processors (Matthew Roark) 2. Histology Openings (Anna Nolan) 3. Paraffin processing native sheep ACL (Elizabeth Ronan) 4. RE: Shandon Excelsior Tissue Processor (Tony Henwood (SCHN)) 5. RE: Shandon Excelsior Tissue Processor (Marcum, Pamela A) 6. Sakura Tissue-Tek Prisma (Casey Arnold) 7. RE: Control Storage (Bitting, Angela K.) 8. Old books (Amber McKenzie) 9. cutting arterial grafts (Megan Elizabeth Flynn) 10. RE: Electron Microscopy protocols (idimitro@mun.ca) ---------------------------------------------------------------------- Message: 1 Date: Wed, 12 Jun 2013 13:18:51 -0500 From: "Matthew Roark" Subject: [Histonet] Tissue Processors To: Message-ID: <000f01ce6799$4a64f2b0$df2ed810$@net> Content-Type: text/plain; charset="us-ascii" So in the next couple of months we are getting ready to demo Thermo's STP 420ES and Leica's ASP6025 tissue processor . Looking for the good, bad, and ugly about them. So if you have one or have had a demo please share what you've found. Thanks!! Matthew Roark- HT/HTL(ASCP)CM Histology Specialist Saint Francis Medical Center 211 Saint Francis Drive Cape Girardeau, MO 63703 573-331-5267 mroark@sfmc.net http://www.sfmc.net ------------------------------ Message: 2 Date: Wed, 12 Jun 2013 15:19:34 -0400 From: "Anna Nolan" Subject: [Histonet] Histology Openings To: Message-ID: <00c801ce67a1$c696cbd0$53c46370$@prometheushealthcare.com> Content-Type: text/plain; charset="us-ascii" Hi my name is Anna and I work with Prometheus Healthcare doing laboratory recruitment. Currently I'm looking to fill several histology positions. Please call or email me if any of these positions are of interest to you. Portland, ME -Histology Technologist (3AM-11AM) NYC -Grossing Specialist (3rd shift) -Histology Tech (1st shift) Suffern, NY -IHC Histology Tech (1st shift) -Cutting embedding, staining, and coverslipping. IHC Experience a plus. (7AM-4PM) Anna Nolan Recruiter Prometheus Healthcare Office 301-693-9057 Cell 301-693-8908 Fax 301-368-2478 anolan @prometheushealthcare.com http://www.linkedin.com/pub/annelise-nolan/55/ba0/ab6 www.prometheushealthcare.com ------------------------------ Message: 3 Date: Wed, 12 Jun 2013 16:59:52 -0400 From: Elizabeth Ronan Subject: [Histonet] Paraffin processing native sheep ACL To: histonet@lists.utsouthwestern.edu Message-ID: <51B8E148.3020102@umich.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Hello, I need to paraffin process native sheep anterior cruciate ligament (ACL) that has been fixed in 10% neutral buffered formalin for 7 days. I was wondering if anyone with more expertise on this subject could guide me with the best lengths of times in the alcohols, xylenes, and paraffin to fix ACL. I tried a 12 hour program but the sections crumbled in the middle and it appeared that the paraffin had not fully perfused the ligament. I have access to the following program, and can alter the lengths of the steps for as long as desired: Program: 70% 80% 95% 95% 100% 100% Xylene Xylene Paraffin Paraffin Paraffin Any advice is much appreciated. Thanks for your time, Liz ------------------------------ Message: 4 Date: Wed, 12 Jun 2013 23:40:22 +0000 From: "Tony Henwood (SCHN)" Subject: RE: [Histonet] Shandon Excelsior Tissue Processor To: "'Velez, Vanessa'" , "histonet@lists.utsouthwestern.edu" Message-ID: <6D6BD1DE8A5571489398B392A38A71579D271C66@xmdb04.nch.kids> Content-Type: text/plain; charset="us-ascii" Vanessa, We installed an Eaton 9130 UPS unit with a SSFIO Surge Protector. Cost under $1,500 Australian. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [ mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Velez, Vanessa Sent: Thursday, 13 June 2013 2:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Shandon Excelsior Tissue Processor Does any one having problems with the Excelsior Tissue Processor power board repeatedly after power outage? This has happened to me three times in the last five years even though it has backup batteries. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* ------------------------------ Message: 5 Date: Thu, 13 Jun 2013 11:05:48 +0000 From: "Marcum, Pamela A" Subject: RE: [Histonet] Shandon Excelsior Tissue Processor To: "Tony Henwood (SCHN)" , "'Velez, Vanessa'" , "histonet@lists.utsouthwestern.edu" Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA32CA17004C@Mail2Node2.ad.uams.edu> Content-Type: text/plain; charset="us-ascii" Since we have some power issues here and 4 Excelsiors (no problems with them on this issue) we have UPS units on all of our computerized equipment. We did this due to issues in other areas of the lab and decided it was safer than taking a chance and being down. We bought Powervar and I think the suggested units were around $2,000 to $3,000 each with one unit serving two processors. Other sites in Little Rock who have some of the same power issues have had problems. Pam Marcum UAMS ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Tony Henwood (SCHN) [tony.henwood@health.nsw.gov.au] Sent: Wednesday, June 12, 2013 6:40 PM To: 'Velez, Vanessa'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Shandon Excelsior Tissue Processor Vanessa, We installed an Eaton 9130 UPS unit with a SSFIO Surge Protector. Cost under $1,500 Australian. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [ mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Velez, Vanessa Sent: Thursday, 13 June 2013 2:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Shandon Excelsior Tissue Processor Does any one having problems with the Excelsior Tissue Processor power board repeatedly after power outage? This has happened to me three times in the last five years even though it has backup batteries. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ------------------------------ Message: 6 Date: Thu, 13 Jun 2013 08:15:23 -0500 From: Casey Arnold Subject: [Histonet] Sakura Tissue-Tek Prisma To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 We have recently started using a Sakura Tissue-tek Prisma that we inherited from our sister lab. While running some validation testing I have noticed that the staining is very inconsistent. The tissue will stain from light to dark within the same rack and sometimes within the same cut (slide). I have reset the mix, speed, etc, to factory settings. I have tried increasing my hematoxylin, and bluing times. I have changed water filters, decreased water rinse times, tried different types of tissue, all to no avail. Has anyone had this problem or have any suggestions on how to correct it? I have tried everything the Sakura tech line has recommended and am still at a loss. -- NOTICE: This electronic mail message and any files transmitted with it are intended exclusively for the individual or entity to which it is addressed. The message, together with any attachment, may contain confidential and/or privileged information. Any unauthorized review, use, printing, saving, copying, disclosure or distribution is strictly prohibited. If you have received this message in error, please immediately advise the sender by reply email and delete all copies. Thank you *Casey Arnold * *Histology Supervisor** OURLabs / OPKO Diagnostics, LLC* 1450 Elm Hill Pike Nashville, TN 37210 Direct: 615-345-4582 Office: 615-874-0410 Fax: 615-345-4595 ------------------------------ Message: 7 Date: Thu, 13 Jun 2013 14:21:16 +0000 From: "Bitting, Angela K." Subject: [Histonet] RE: Control Storage To: Elizabeth Chlipala , "Herring, Colleen" , "Histonet@lists.utsouthwestern.edu" Message-ID: <77F52EFAB8B1694B885E277C48FCD0F647C7298D@GHSEXMBX4W8K1V.geisinger.edu> Content-Type: text/plain; charset="us-ascii" Something to remember is that humidity is the enemy too. If you are storing your slides in a refrigerator make sure that the humidity is well controlled. We found out the hard way. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [ mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala Sent: Wednesday, June 12, 2013 11:56 AM To: Herring, Colleen; Histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Control Storage We store our blocks at room temp, our IHC slides are stored in the fridge. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 881-0763 cell (303) 682-9060 fax liz@premierlab.com Ship to address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Herring, Colleen [Colleen_Herring@bshsi.org] Sent: Wednesday, June 12, 2013 9:48 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Control Storage Does anyone have any ideas or suggestions about the storage of blocks and precut slides for immunos? ________________________________________________________________________________________________________ The information in this communication is intended to be confidential to the Individual(s) and/or Entity to whom it is addressed. It may contain information of a Privileged and/or Confidential nature, which is subject to Federal and/or State privacy regulations. In the event that you are not the intended recipient or the agent of the intended recipient, do not copy or use the information contained within this communication, or allow it to be read, copied or utilized in any manner, by any other person(s). Should this communication be received in error, please notify the sender immediately either by response e-mail or by phone, and permanently delete the original e-mail, attachment(s), and any copies. ________________________________________________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. 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If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. ------------------------------------------------------------------------- This message was secured by ZixCorp(R). ------------------------------ Message: 8 Date: Thu, 13 Jun 2013 14:22:10 +0000 From: Amber McKenzie Subject: [Histonet] Old books To: "histonet@lists.utsouthwestern.edu" Message-ID: <5A33C952BB67F4468AF1F36D739212BCBD6ADF63@JERRY.Gia.com> Content-Type: text/plain; charset="us-ascii" I'm cleaning house :) Anyone interested in buying these old books? 1. A color Atlas of Histology by Dennis Strete, ISBN 0-06-501084-1 2. Human Histology, 2nd edition, by Alan Stevens and James Lowe, ISBN 0-7234-2485-3 3. Immunolgy, 4th edition, by Roitt, Brostoff, Male ISBN 0-7234-2178-1 4. Atlas of Histology with Funcitonal correlations, by Victor P. Erodschenko ISBN 0-583-2818-9 5. Histotechnology, A Self Instructional Text, 2nd edition, by Freida Carson (I'm interested in purchasing the 3rd edition). ------------------------------ Message: 9 Date: Thu, 13 Jun 2013 15:11:12 +0000 From: Megan Elizabeth Flynn Subject: [Histonet] cutting arterial grafts To: "histonet@lists.utsouthwestern.edu" Message-ID: <5748287483CD8A48BC4A27D05908D4E215E72FFC@CHCSPMBX3.ads.northwestern.edu> Content-Type: text/plain; charset="us-ascii" Hi all, I am in a research lab and I am cutting rat arterial grafts made with ePTFE. I'm cutting at 30 degrees at 5 microns. The lower edge of the graft seems to be lifting up from the adventitia and folding over. I have tried playing with the temperature, but none of it seems to make a difference. Can anyone help? Megan ------------------------------ Message: 10 Date: Thu, 13 Jun 2013 15:55:23 +0000 From: Subject: RE: [Histonet] Electron Microscopy protocols To: , Message-ID: <14C3108E8B98EF43B3EDAE58336700341FA60F@exchange.med.mun.ca> Content-Type: text/plain; charset="us-ascii" Hi Joel, Lipids are fixed only by OsO4, so you need to use it as a post-fixative. As a fixative in our lab we use Karnovsky fixative, then we use osmium tetroxide as a post-fixative/fixative for the lipids for 15 min. Then we wash in Na-cacodylate buffer for 5 min, and then dehydrate, starting with two changes of 70% ethanol ( for 10min.) and then we go to two changes of 95% and then absolute ethanol, followed by two changes of abs. acetone, then 50:50 acetone:resin and 2 changes of resin, then embedding and polymerization at the right temperature. If your lipids are fixed properly they will be round droplets with characteristic appearance. OsO4 will blacken the tissue. We always have excellent results with this protocol. Hope that was helpful, Iliana dimitrova, Memorial university of Newfoundland, Canada -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [ mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joel Haas Sent: June-05-13 3:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Electron Microscopy protocols Hello all, I am working on developing an electron microscopy protocol for looking at lipid droplets in cultured cells. We have used thin section TEM previously and found that the morphology of the droplets is often deformed (should be spherical, shows up as egg shaped or lumpy). If possible we would like to avoid these types of preservation artifacts. Does anyone have suggestions for adapting the protocol to better preserve these types of structures--a triglyceride lipid core surrounded by a phospholipid monolayer? Thanks in advance, Joel _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic communication is governed by the terms and conditions at http://www.mun.ca/cc/policies/electronic_communications_disclaimer_2012.php ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 115, Issue 13 ***************************************** From SSCALISE <@t> beaumont.edu Thu Jun 13 20:34:12 2013 From: SSCALISE <@t> beaumont.edu (Sharon Scalise) Date: Thu Jun 13 20:34:16 2013 Subject: [Histonet] PA job opening Message-ID: <190D98228ADC1747BCE27019B78FD8F301165ECB@EXMAIL06.ms.beaumont.edu> We currently have an opening for a part-time contingent Pathologists' Assistant at our Royal Oak, Michigan campus. Please go to www.beaumont.edu and click on the "careers" tab for more information. Sharon Scalise, HTL(ASCP) Histology Supervisor-Anatomic Pathology Beaumont Health System 3601 W. 13 Mile Rd. Royal Oak, MI 48073 248 898-5981 sscalise@beaumont.edu From a.prior <@t> tissueregenix.com Fri Jun 14 02:45:47 2013 From: a.prior <@t> tissueregenix.com (Andrew Prior) Date: Fri Jun 14 02:42:58 2013 Subject: [Histonet] Paraffin processing native sheep ACL Message-ID: Hi Liz, I inherited the following protocol for ACL samples. It works quite well, but times probably could be reduced - the optimisation is on my to-do list. 70% Alcohol - 1 hour 90% alcohol - 1 hour 100% Alcohol -2 hours 100% alcohol - 3 hours 100% alcohol - 4 hours Xylene - 1.5 hours Xylene - 1.5 hours Xylene - 3 hours Wax - 3 hours Wax - 3 hours Wax - 4 hours I cut the sections at 8um so they hold together better. Takes a while for all the wrinkles to disappear when floating out on water-bath so be patient Hope this helps. Andrew Andrew Prior Histologist Tissue Regenix Group E-mail: a.prior@tissueregenix.com Website: www.tissueregenix.com ------------------------------ Message: 3 Date: Wed, 12 Jun 2013 16:59:52 -0400 From: Elizabeth Ronan > Subject: [Histonet] Paraffin processing native sheep ACL To: histonet@lists.utsouthwestern.edu Message-ID: <51B8E148.3020102@umich.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Hello, I need to paraffin process native sheep anterior cruciate ligament (ACL) that has been fixed in 10% neutral buffered formalin for 7 days. I was wondering if anyone with more expertise on this subject could guide me with the best lengths of times in the alcohols, xylenes, and paraffin to fix ACL. I tried a 12 hour program but the sections crumbled in the middle and it appeared that the paraffin had not fully perfused the ligament. I have access to the following program, and can alter the lengths of the steps for as long as desired: Program: 70% 80% 95% 95% 100% 100% Xylene Xylene Paraffin Paraffin Paraffin Any advice is much appreciated. Thanks for your time, Liz The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited. If you received this in error, please contact the sender and delete the material from any computer. Although we routinely screen for viruses, addressees should check this e-mail and any attachment for viruses. We make no warranty as to absence of viruses in this e-mail or any attachments. Registered Office: The Biocentre, Innovation Way, Heslington, York, YO10 5NY Registered No. 05969271 From mucram11 <@t> comcast.net Fri Jun 14 10:40:28 2013 From: mucram11 <@t> comcast.net (Pam Marcum) Date: Fri Jun 14 10:40:50 2013 Subject: [Histonet] Re: [Sorry cannot send without piggybacking this) In-Reply-To: Message-ID: <87624226.270636.1371224428471.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> We are in the process of installing EPIC University wide at the Medical Center.? I seem to be seeing some issues with EPIC understanding AP and what we need to run a Histology/IHC/Slide and Block/Gross Room and Morgue and several other areas.? I am getting the impression this is a system for the pathologists and doctors with all areas of lab sort of an afterthought.? Can anyone using give me feedback offline or online?? I am going in as superuser and need to know what I am facing.? The only other choice we have is to stay with an older version of SoftPath, UGH! ? Thank you for any feelback, Pam Marcum ----- Original Message ----- From: "WILLIAM DESALVO" To: "Akemi Allison" , "histonet" Sent: Thursday, April 4, 2013 11:53:42 AM Subject: RE: [Histonet] CEU's 36 hours in three years. ? Go to: http://www.ascp.org/Board-of-Certification William DeSalvo, BS HTL(ASCP) Production Manager-Anatomic Pathology Chair, NSH Quality Management Committee Owner/Consultant, Collaborative Advantage Consulting ? > Date: Thu, 4 Apr 2013 09:32:52 -0700 > From: akemiat3377@yahoo.com > To: Histonet@lists.utsouthwestern.edu > CC: > Subject: [Histonet] CEU's > > Hi All: > ? > I have been asked a question that I don't have the answer too. I need to know how many CEU's a registered HT/HTL is required to have to maintain their registry. ?I am a dinosaur who received her HT in 1968 and my HTL in 1982, so do not need to renew mine. > ? > Akemi Allison-Tacha, BS, HT (ASCP) HTL > Pathology Manager > Monterey Bay GI Consultants > 23 Upper Ragsdale Drive, Suite 200 > Monterey, CA 93940 > (381) 375-3577 ?X117 > Email: aallison@montereygi.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ????????????????? ???????? ? ???????????????? ?_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ASelf <@t> georgetownhospitalsystem.org Fri Jun 14 11:00:25 2013 From: ASelf <@t> georgetownhospitalsystem.org (Amy Self) Date: Fri Jun 14 11:01:28 2013 Subject: [Histonet] Non-Gyn Cytology Competency Message-ID: Happy Friday to all in Histoland, I am in need of a non-gyn cytology competency checklist. Does anyone out there have anything that they would be willing to share with me? Thanks in advance and I hope everyone has a wonderful weekend. Amy Self Histology Lab Senior Tech Lab Georgetown Memorial Hospital 606 Black River Road Georgetown, SC 29440 843-520-8711 ASelf@georgetownhospitalsystem.org NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. From joewalker <@t> rrmc.org Fri Jun 14 11:23:26 2013 From: joewalker <@t> rrmc.org (Joe W. Walker, Jr.) Date: Fri Jun 14 11:23:32 2013 Subject: [Histonet] RE: Non-Gyn Cytology Competency In-Reply-To: References: Message-ID: <3C2378778400AD448ADA6FD6BDB7CCCC17F6265B@RRMBX03.rrmc.local> Hi Amy, Please define Non-GYN Cytology Competency checklist. Are you talking about the prep of non-gyn's or assessing competency of the cytotechnologist and pathologist in the interpretation of results? Joe W. Walker, Jr. MS, SCT(ASCP)CM Anatomical Pathology Manager Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P: 802.747.1790 F: 802.747.6525 NEW EMAIL: joewalker@rrmc.org http://www.rrmc.org/ Our Vision: To be the Best Community Healthcare System in New England Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet Recognition? and the Governor's Award for Performance Excellence -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Self Sent: Friday, June 14, 2013 12:00 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Non-Gyn Cytology Competency Happy Friday to all in Histoland, I am in need of a non-gyn cytology competency checklist. Does anyone out there have anything that they would be willing to share with me? Thanks in advance and I hope everyone has a wonderful weekend. Amy Self Histology Lab Senior Tech Lab Georgetown Memorial Hospital 606 Black River Road Georgetown, SC 29440 843-520-8711 ASelf@georgetownhospitalsystem.org NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. Thank You From jtaylor <@t> meriter.com Fri Jun 14 12:07:14 2013 From: jtaylor <@t> meriter.com (Taylor, Jean) Date: Fri Jun 14 12:07:24 2013 Subject: [Histonet] p40 staining for breast myoepithelial cells Message-ID: Hi everyone, Are any labs using the monoclonal p40 antibody for myoepithelial cell staining in breast tissue? The pathologist that I work with has me testing this antibody to be used to differentiate between lung squamous and adenocarcinoma, but is also wondering if labs are using it on breast tissue as well. Thanks, Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI From khbarr <@t> mdanderson.org Fri Jun 14 12:17:44 2013 From: khbarr <@t> mdanderson.org (Barr,Kaye H) Date: Fri Jun 14 12:18:34 2013 Subject: [Histonet] RE: Histonet Digest, Vol 115, Issue 14 In-Reply-To: <3d55e2c3-3acc-4a05-8b05-7e8701034646@DCPWPEXHUBCAS05.mdanderson.edu> References: <3d55e2c3-3acc-4a05-8b05-7e8701034646@DCPWPEXHUBCAS05.mdanderson.edu> Message-ID: <65C8EC869E8581459DCD566079572FAC0B7EC491@DCPWPEXMBX02.mdanderson.edu> The University of Texas M. D. Anderson Cancer Center is looking for a part time pathologist assistant. Hours are flexible. If interested, apply at www.mdanderson.org. Click on careers, search current openings. Kaye Barr, HT (ASCP) | Laboratory Manager M. D. Anderson Cancer Center | Pathology/Histology Labs 1515 Holcombe Blvd, Unit 85 | Houston, TX 77030 713.792.5366 office | khbarr@mdanderson.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Friday, June 14, 2013 12:02 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 115, Issue 14 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Histonet Digest, Vol 115, Issue 13 (Andrea.X.Dejager@kp.org) 2. PA job opening (Sharon Scalise) 3. Re: Paraffin processing native sheep ACL (Andrew Prior) 4. Re: [Sorry cannot send without piggybacking this) (Pam Marcum) 5. Non-Gyn Cytology Competency (Amy Self) 6. RE: Non-Gyn Cytology Competency (Joe W. Walker, Jr.) ---------------------------------------------------------------------- Message: 1 Date: Thu, 13 Jun 2013 11:25:39 -0600 From: Andrea.X.Dejager@kp.org Subject: [Histonet] Re: Histonet Digest, Vol 115, Issue 13 To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" This message is for Casey Arnold, we never had any problems with our stainer/coverslipper, I will send you our procedure to try out. Do you dry slides on the stainer or elsewhere? Andrea De Jager, H.T. ASCP Histology Manager, Regional Reference Lab Kaiser Permanente - Colorado Phone: 303-404-4152 Fax: 303-404-4161 email: ANDREA.X.DEJAGER@KP.org NOTICE TO RECIPIENT: If you are not the intended recipient of this e-mail, you are prohibited from sharing, copying, or otherwise using or disclosing its contents. If you have received this e-mail in error, please notify the sender immediately by reply e-mail and permanently delete this e-mail and any attachments without reading, forwarding or saving them. Thank you. From: histonet-request@lists.utsouthwestern.edu To: histonet@lists.utsouthwestern.edu Date: 06/13/2013 11:04 AM Subject: Histonet Digest, Vol 115, Issue 13 Sent by: histonet-bounces@lists.utsouthwestern.edu Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Tissue Processors (Matthew Roark) 2. Histology Openings (Anna Nolan) 3. Paraffin processing native sheep ACL (Elizabeth Ronan) 4. RE: Shandon Excelsior Tissue Processor (Tony Henwood (SCHN)) 5. RE: Shandon Excelsior Tissue Processor (Marcum, Pamela A) 6. Sakura Tissue-Tek Prisma (Casey Arnold) 7. RE: Control Storage (Bitting, Angela K.) 8. Old books (Amber McKenzie) 9. cutting arterial grafts (Megan Elizabeth Flynn) 10. RE: Electron Microscopy protocols (idimitro@mun.ca) ---------------------------------------------------------------------- Message: 1 Date: Wed, 12 Jun 2013 13:18:51 -0500 From: "Matthew Roark" Subject: [Histonet] Tissue Processors To: Message-ID: <000f01ce6799$4a64f2b0$df2ed810$@net> Content-Type: text/plain; charset="us-ascii" So in the next couple of months we are getting ready to demo Thermo's STP 420ES and Leica's ASP6025 tissue processor . Looking for the good, bad, and ugly about them. So if you have one or have had a demo please share what you've found. Thanks!! Matthew Roark- HT/HTL(ASCP)CM Histology Specialist Saint Francis Medical Center 211 Saint Francis Drive Cape Girardeau, MO 63703 573-331-5267 mroark@sfmc.net http://www.sfmc.net ------------------------------ Message: 2 Date: Wed, 12 Jun 2013 15:19:34 -0400 From: "Anna Nolan" Subject: [Histonet] Histology Openings To: Message-ID: <00c801ce67a1$c696cbd0$53c46370$@prometheushealthcare.com> Content-Type: text/plain; charset="us-ascii" Hi my name is Anna and I work with Prometheus Healthcare doing laboratory recruitment. Currently I'm looking to fill several histology positions. Please call or email me if any of these positions are of interest to you. Portland, ME -Histology Technologist (3AM-11AM) NYC -Grossing Specialist (3rd shift) -Histology Tech (1st shift) Suffern, NY -IHC Histology Tech (1st shift) -Cutting embedding, staining, and coverslipping. IHC Experience a plus. (7AM-4PM) Anna Nolan Recruiter Prometheus Healthcare Office 301-693-9057 Cell 301-693-8908 Fax 301-368-2478 anolan @prometheushealthcare.com http://www.linkedin.com/pub/annelise-nolan/55/ba0/ab6 www.prometheushealthcare.com ------------------------------ Message: 3 Date: Wed, 12 Jun 2013 16:59:52 -0400 From: Elizabeth Ronan Subject: [Histonet] Paraffin processing native sheep ACL To: histonet@lists.utsouthwestern.edu Message-ID: <51B8E148.3020102@umich.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Hello, I need to paraffin process native sheep anterior cruciate ligament (ACL) that has been fixed in 10% neutral buffered formalin for 7 days. I was wondering if anyone with more expertise on this subject could guide me with the best lengths of times in the alcohols, xylenes, and paraffin to fix ACL. I tried a 12 hour program but the sections crumbled in the middle and it appeared that the paraffin had not fully perfused the ligament. I have access to the following program, and can alter the lengths of the steps for as long as desired: Program: 70% 80% 95% 95% 100% 100% Xylene Xylene Paraffin Paraffin Paraffin Any advice is much appreciated. Thanks for your time, Liz ------------------------------ Message: 4 Date: Wed, 12 Jun 2013 23:40:22 +0000 From: "Tony Henwood (SCHN)" Subject: RE: [Histonet] Shandon Excelsior Tissue Processor To: "'Velez, Vanessa'" , "histonet@lists.utsouthwestern.edu" Message-ID: <6D6BD1DE8A5571489398B392A38A71579D271C66@xmdb04.nch.kids> Content-Type: text/plain; charset="us-ascii" Vanessa, We installed an Eaton 9130 UPS unit with a SSFIO Surge Protector. Cost under $1,500 Australian. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [ mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Velez, Vanessa Sent: Thursday, 13 June 2013 2:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Shandon Excelsior Tissue Processor Does any one having problems with the Excelsior Tissue Processor power board repeatedly after power outage? This has happened to me three times in the last five years even though it has backup batteries. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* ------------------------------ Message: 5 Date: Thu, 13 Jun 2013 11:05:48 +0000 From: "Marcum, Pamela A" Subject: RE: [Histonet] Shandon Excelsior Tissue Processor To: "Tony Henwood (SCHN)" , "'Velez, Vanessa'" , "histonet@lists.utsouthwestern.edu" Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA32CA17004C@Mail2Node2.ad.uams.edu> Content-Type: text/plain; charset="us-ascii" Since we have some power issues here and 4 Excelsiors (no problems with them on this issue) we have UPS units on all of our computerized equipment. We did this due to issues in other areas of the lab and decided it was safer than taking a chance and being down. We bought Powervar and I think the suggested units were around $2,000 to $3,000 each with one unit serving two processors. Other sites in Little Rock who have some of the same power issues have had problems. Pam Marcum UAMS ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Tony Henwood (SCHN) [tony.henwood@health.nsw.gov.au] Sent: Wednesday, June 12, 2013 6:40 PM To: 'Velez, Vanessa'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Shandon Excelsior Tissue Processor Vanessa, We installed an Eaton 9130 UPS unit with a SSFIO Surge Protector. Cost under $1,500 Australian. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [ mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Velez, Vanessa Sent: Thursday, 13 June 2013 2:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Shandon Excelsior Tissue Processor Does any one having problems with the Excelsior Tissue Processor power board repeatedly after power outage? This has happened to me three times in the last five years even though it has backup batteries. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ------------------------------ Message: 6 Date: Thu, 13 Jun 2013 08:15:23 -0500 From: Casey Arnold Subject: [Histonet] Sakura Tissue-Tek Prisma To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 We have recently started using a Sakura Tissue-tek Prisma that we inherited from our sister lab. While running some validation testing I have noticed that the staining is very inconsistent. The tissue will stain from light to dark within the same rack and sometimes within the same cut (slide). I have reset the mix, speed, etc, to factory settings. I have tried increasing my hematoxylin, and bluing times. I have changed water filters, decreased water rinse times, tried different types of tissue, all to no avail. Has anyone had this problem or have any suggestions on how to correct it? I have tried everything the Sakura tech line has recommended and am still at a loss. -- NOTICE: This electronic mail message and any files transmitted with it are intended exclusively for the individual or entity to which it is addressed. The message, together with any attachment, may contain confidential and/or privileged information. Any unauthorized review, use, printing, saving, copying, disclosure or distribution is strictly prohibited. If you have received this message in error, please immediately advise the sender by reply email and delete all copies. Thank you *Casey Arnold * *Histology Supervisor** OURLabs / OPKO Diagnostics, LLC* 1450 Elm Hill Pike Nashville, TN 37210 Direct: 615-345-4582 Office: 615-874-0410 Fax: 615-345-4595 ------------------------------ Message: 7 Date: Thu, 13 Jun 2013 14:21:16 +0000 From: "Bitting, Angela K." Subject: [Histonet] RE: Control Storage To: Elizabeth Chlipala , "Herring, Colleen" , "Histonet@lists.utsouthwestern.edu" Message-ID: <77F52EFAB8B1694B885E277C48FCD0F647C7298D@GHSEXMBX4W8K1V.geisinger.edu> Content-Type: text/plain; charset="us-ascii" Something to remember is that humidity is the enemy too. If you are storing your slides in a refrigerator make sure that the humidity is well controlled. We found out the hard way. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [ mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala Sent: Wednesday, June 12, 2013 11:56 AM To: Herring, Colleen; Histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Control Storage We store our blocks at room temp, our IHC slides are stored in the fridge. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 881-0763 cell (303) 682-9060 fax liz@premierlab.com Ship to address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Herring, Colleen [Colleen_Herring@bshsi.org] Sent: Wednesday, June 12, 2013 9:48 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Control Storage Does anyone have any ideas or suggestions about the storage of blocks and precut slides for immunos? ________________________________________________________________________________________________________ The information in this communication is intended to be confidential to the Individual(s) and/or Entity to whom it is addressed. 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Should this communication be received in error, please notify the sender immediately either by response e-mail or by phone, and permanently delete the original e-mail, attachment(s), and any copies. ________________________________________________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. 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If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. ------------------------------------------------------------------------- This message was secured by ZixCorp(R). ------------------------------ Message: 8 Date: Thu, 13 Jun 2013 14:22:10 +0000 From: Amber McKenzie Subject: [Histonet] Old books To: "histonet@lists.utsouthwestern.edu" Message-ID: <5A33C952BB67F4468AF1F36D739212BCBD6ADF63@JERRY.Gia.com> Content-Type: text/plain; charset="us-ascii" I'm cleaning house :) Anyone interested in buying these old books? 1. A color Atlas of Histology by Dennis Strete, ISBN 0-06-501084-1 2. Human Histology, 2nd edition, by Alan Stevens and James Lowe, ISBN 0-7234-2485-3 3. Immunolgy, 4th edition, by Roitt, Brostoff, Male ISBN 0-7234-2178-1 4. Atlas of Histology with Funcitonal correlations, by Victor P. Erodschenko ISBN 0-583-2818-9 5. Histotechnology, A Self Instructional Text, 2nd edition, by Freida Carson (I'm interested in purchasing the 3rd edition). ------------------------------ Message: 9 Date: Thu, 13 Jun 2013 15:11:12 +0000 From: Megan Elizabeth Flynn Subject: [Histonet] cutting arterial grafts To: "histonet@lists.utsouthwestern.edu" Message-ID: <5748287483CD8A48BC4A27D05908D4E215E72FFC@CHCSPMBX3.ads.northwestern.edu> Content-Type: text/plain; charset="us-ascii" Hi all, I am in a research lab and I am cutting rat arterial grafts made with ePTFE. I'm cutting at 30 degrees at 5 microns. The lower edge of the graft seems to be lifting up from the adventitia and folding over. I have tried playing with the temperature, but none of it seems to make a difference. Can anyone help? Megan ------------------------------ Message: 10 Date: Thu, 13 Jun 2013 15:55:23 +0000 From: Subject: RE: [Histonet] Electron Microscopy protocols To: , Message-ID: <14C3108E8B98EF43B3EDAE58336700341FA60F@exchange.med.mun.ca> Content-Type: text/plain; charset="us-ascii" Hi Joel, Lipids are fixed only by OsO4, so you need to use it as a post-fixative. As a fixative in our lab we use Karnovsky fixative, then we use osmium tetroxide as a post-fixative/fixative for the lipids for 15 min. Then we wash in Na-cacodylate buffer for 5 min, and then dehydrate, starting with two changes of 70% ethanol ( for 10min.) and then we go to two changes of 95% and then absolute ethanol, followed by two changes of abs. acetone, then 50:50 acetone:resin and 2 changes of resin, then embedding and polymerization at the right temperature. If your lipids are fixed properly they will be round droplets with characteristic appearance. OsO4 will blacken the tissue. We always have excellent results with this protocol. Hope that was helpful, Iliana dimitrova, Memorial university of Newfoundland, Canada -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [ mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joel Haas Sent: June-05-13 3:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Electron Microscopy protocols Hello all, I am working on developing an electron microscopy protocol for looking at lipid droplets in cultured cells. We have used thin section TEM previously and found that the morphology of the droplets is often deformed (should be spherical, shows up as egg shaped or lumpy). If possible we would like to avoid these types of preservation artifacts. Does anyone have suggestions for adapting the protocol to better preserve these types of structures--a triglyceride lipid core surrounded by a phospholipid monolayer? Thanks in advance, Joel _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic communication is governed by the terms and conditions at http://www.mun.ca/cc/policies/electronic_communications_disclaimer_2012.php ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 115, Issue 13 ***************************************** ------------------------------ Message: 2 Date: Fri, 14 Jun 2013 01:34:12 +0000 From: Sharon Scalise Subject: [Histonet] PA job opening To: "'histonet' (histonet@lists.utsouthwestern.edu)" Message-ID: <190D98228ADC1747BCE27019B78FD8F301165ECB@EXMAIL06.ms.beaumont.edu> Content-Type: text/plain; charset="us-ascii" We currently have an opening for a part-time contingent Pathologists' Assistant at our Royal Oak, Michigan campus. Please go to www.beaumont.edu and click on the "careers" tab for more information. Sharon Scalise, HTL(ASCP) Histology Supervisor-Anatomic Pathology Beaumont Health System 3601 W. 13 Mile Rd. Royal Oak, MI 48073 248 898-5981 sscalise@beaumont.edu ------------------------------ Message: 3 Date: Fri, 14 Jun 2013 07:45:47 +0000 From: Andrew Prior Subject: Re: [Histonet] Paraffin processing native sheep ACL To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Hi Liz, I inherited the following protocol for ACL samples. It works quite well, but times probably could be reduced - the optimisation is on my to-do list. 70% Alcohol - 1 hour 90% alcohol - 1 hour 100% Alcohol -2 hours 100% alcohol - 3 hours 100% alcohol - 4 hours Xylene - 1.5 hours Xylene - 1.5 hours Xylene - 3 hours Wax - 3 hours Wax - 3 hours Wax - 4 hours I cut the sections at 8um so they hold together better. Takes a while for all the wrinkles to disappear when floating out on water-bath so be patient Hope this helps. Andrew Andrew Prior Histologist Tissue Regenix Group E-mail: a.prior@tissueregenix.com Website: www.tissueregenix.com ------------------------------ Message: 3 Date: Wed, 12 Jun 2013 16:59:52 -0400 From: Elizabeth Ronan > Subject: [Histonet] Paraffin processing native sheep ACL To: histonet@lists.utsouthwestern.edu Message-ID: <51B8E148.3020102@umich.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Hello, I need to paraffin process native sheep anterior cruciate ligament (ACL) that has been fixed in 10% neutral buffered formalin for 7 days. I was wondering if anyone with more expertise on this subject could guide me with the best lengths of times in the alcohols, xylenes, and paraffin to fix ACL. I tried a 12 hour program but the sections crumbled in the middle and it appeared that the paraffin had not fully perfused the ligament. I have access to the following program, and can alter the lengths of the steps for as long as desired: Program: 70% 80% 95% 95% 100% 100% Xylene Xylene Paraffin Paraffin Paraffin Any advice is much appreciated. Thanks for your time, Liz The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited. If you received this in error, please contact the sender and delete the material from any computer. Although we routinely screen for viruses, addressees should check this e-mail and any attachment for viruses. We make no warranty as to absence of viruses in this e-mail or any attachments. Registered Office: The Biocentre, Innovation Way, Heslington, York, YO10 5NY Registered No. 05969271 ------------------------------ Message: 4 Date: Fri, 14 Jun 2013 15:40:28 +0000 (UTC) From: Pam Marcum Subject: [Histonet] Re: [Sorry cannot send without piggybacking this) To: WILLIAM DESALVO Cc: histonet Message-ID: <87624226.270636.1371224428471.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Content-Type: text/plain; charset=utf-8 We are in the process of installing EPIC University wide at the Medical Center.?? I seem to be seeing some issues with EPIC understanding AP and what we need to run a Histology/IHC/Slide and Block/Gross Room and Morgue and several other areas.?? I am getting the impression this is a system for the pathologists and doctors with all areas of lab sort of an afterthought.?? Can anyone using give me feedback offline or online??? I am going in as superuser and need to know what I am facing.?? The only other choice we have is to stay with an older version of SoftPath, UGH! ?? Thank you for any feelback, Pam Marcum ----- Original Message ----- From: "WILLIAM DESALVO" To: "Akemi Allison" , "histonet" Sent: Thursday, April 4, 2013 11:53:42 AM Subject: RE: [Histonet] CEU's 36 hours in three years. ?? Go to: http://www.ascp.org/Board-of-Certification William DeSalvo, BS HTL(ASCP) Production Manager-Anatomic Pathology Chair, NSH Quality Management Committee Owner/Consultant, Collaborative Advantage Consulting ?? > Date: Thu, 4 Apr 2013 09:32:52 -0700 > From: akemiat3377@yahoo.com > To: Histonet@lists.utsouthwestern.edu > CC: > Subject: [Histonet] CEU's > > Hi All: > ?? > I have been asked a question that I don't have the answer too. I need to know how many CEU's a registered HT/HTL is required to have to maintain their registry. ??I am a dinosaur who received her HT in 1968 and my HTL in 1982, so do not need to renew mine. > ?? > Akemi Allison-Tacha, BS, HT (ASCP) HTL > Pathology Manager > Monterey Bay GI Consultants > 23 Upper Ragsdale Drive, Suite 200 > Monterey, CA 93940 > (381) 375-3577 ??X117 > Email: aallison@montereygi.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ?????????????????????????????????? ???????????????? ?? ???????????????????????????????? ??_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Fri, 14 Jun 2013 12:00:25 -0400 From: Amy Self Subject: [Histonet] Non-Gyn Cytology Competency To: "'histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain; charset="us-ascii" Happy Friday to all in Histoland, I am in need of a non-gyn cytology competency checklist. Does anyone out there have anything that they would be willing to share with me? Thanks in advance and I hope everyone has a wonderful weekend. Amy Self Histology Lab Senior Tech Lab Georgetown Memorial Hospital 606 Black River Road Georgetown, SC 29440 843-520-8711 ASelf@georgetownhospitalsystem.org NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. ------------------------------ Message: 6 Date: Fri, 14 Jun 2013 16:23:26 +0000 From: "Joe W. Walker, Jr." Subject: [Histonet] RE: Non-Gyn Cytology Competency To: Amy Self , "'histonet@lists.utsouthwestern.edu'" Message-ID: <3C2378778400AD448ADA6FD6BDB7CCCC17F6265B@RRMBX03.rrmc.local> Content-Type: text/plain; charset="iso-8859-1" Hi Amy, Please define Non-GYN Cytology Competency checklist. Are you talking about the prep of non-gyn's or assessing competency of the cytotechnologist and pathologist in the interpretation of results? Joe W. Walker, Jr. MS, SCT(ASCP)CM Anatomical Pathology Manager Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P: 802.747.1790 F: 802.747.6525 NEW EMAIL: joewalker@rrmc.org http://www.rrmc.org/ Our Vision: To be the Best Community Healthcare System in New England Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet Recognition? and the Governor's Award for Performance Excellence -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Self Sent: Friday, June 14, 2013 12:00 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Non-Gyn Cytology Competency Happy Friday to all in Histoland, I am in need of a non-gyn cytology competency checklist. Does anyone out there have anything that they would be willing to share with me? Thanks in advance and I hope everyone has a wonderful weekend. Amy Self Histology Lab Senior Tech Lab Georgetown Memorial Hospital 606 Black River Road Georgetown, SC 29440 843-520-8711 ASelf@georgetownhospitalsystem.org NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. Thank You ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 115, Issue 14 ***************************************** From Rcartun <@t> harthosp.org Fri Jun 14 12:36:14 2013 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Jun 14 12:36:32 2013 Subject: [Histonet] Re: [IHCRG] p40 staining for breast myoepithelial cells In-Reply-To: References: Message-ID: <51BB1C4E020000770003BDAD@gwmail3.harthosp.org> We have not validated it for this purpose, but it does label myoepithelial cells in breast tissue very nicely. FYI - We are using BioCare's "RTU" p40 on Leica's Bond Max. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> "Taylor, Jean" 6/14/2013 1:07 PM >>> Hi everyone, Are any labs using the monoclonal p40 antibody for myoepithelial cell staining in breast tissue? The pathologist that I work with has me testing this antibody to be used to differentiate between lung squamous and adenocarcinoma, but is also wondering if labs are using it on breast tissue as well. Thanks, Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI -- -- You received this message because you are subscribed to the Google Groups "ihcrg" group. The IHC Resource Group is a standing committee within the National Society for Histotechnology. To post to this group, send email to ihcrg@googlegroups.com To unsubscribe from this group, send email to ihcrg+unsubscribe@googlegroups.com For more options, visit this group at http://groups.google.com/group/ihcrg?hl=en To contact the National Society for Histotechnology, email: histo@nsh.org or call 443.535.4060. --- You received this message because you are subscribed to the Google Groups "ihcrg" group. To unsubscribe from this group and stop receiving emails from it, send an email to ihcrg+unsubscribe@googlegroups.com. For more options, visit https://groups.google.com/groups/opt_out. From brannon <@t> alliedsearchpartners.com Fri Jun 14 14:30:09 2013 From: brannon <@t> alliedsearchpartners.com (Brannon Owens) Date: Fri Jun 14 14:30:21 2013 Subject: [Histonet] Part Time HT/HTL Opening Message-ID: We are now hiring for a part time permanent HT or HTL for a laboratory in the northwest Atlanta, GA area. Send email to brannon@alliedsearchpartners.com for a full job description or to apply. Brannon Owens Recruitment Manager Allied Search Partners LinkedIn: http://www.linkedin.com/pub/brannon-owens/28/528/823 http://www.alliedsearchpartners.com T: 888.388.7571 ext. 106 F: 888.388.7572 From KJohnson <@t> med.miami.edu Fri Jun 14 15:09:20 2013 From: KJohnson <@t> med.miami.edu (Johnson, Kevin) Date: Fri Jun 14 15:09:37 2013 Subject: [Histonet] Cutting paraffin sections...on a cryostat? Message-ID: <59F7809368E0084C8AE6B38C93D735EB2FA3D609C3@MEDEXMB02.ad.med.miami.edu> Hi, all. A bit of an odd question: a colleague knows of someone wanting to cut paraffin sections who has a cryostat, but no microtome. Since a cryostat's basically a microtome in a freezer chamber, I thought that it may be awkward, but theoretically doable once it was brought to room temp and dried out thoroughly. However, I wondered if lubricants formulated for the cold might become too thin for use at room temp, possibly causing damage to moving parts. Any thoughts? Kevin Johnson University of Miami Diabetes Research Institute From sdysart <@t> mirnarx.com Fri Jun 14 15:14:59 2013 From: sdysart <@t> mirnarx.com (Sarah Dysart) Date: Fri Jun 14 15:15:06 2013 Subject: [Histonet] RE: Cutting paraffin sections...on a cryostat? In-Reply-To: <59F7809368E0084C8AE6B38C93D735EB2FA3D609C3@MEDEXMB02.ad.med.miami.edu> References: <59F7809368E0084C8AE6B38C93D735EB2FA3D609C3@MEDEXMB02.ad.med.miami.edu> Message-ID: <8cfd4dd5a99d4f2aa6dab07cc650b673@BY2PR07MB010.namprd07.prod.outlook.com> Technically you are right...it's just a really cold tome. I guess you could cut sections with it...it would be super awkward though you're right. I wouldn't use normal tome oil on it because if you did decide to turn it back on, the normal oil will freeze up on you. Just continue to use the cryostat oil and you should be fine. Tell your colleague good luck!! Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Johnson, Kevin Sent: Friday, June 14, 2013 3:09 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Cutting paraffin sections...on a cryostat? Hi, all. A bit of an odd question: a colleague knows of someone wanting to cut paraffin sections who has a cryostat, but no microtome. Since a cryostat's basically a microtome in a freezer chamber, I thought that it may be awkward, but theoretically doable once it was brought to room temp and dried out thoroughly. However, I wondered if lubricants formulated for the cold might become too thin for use at room temp, possibly causing damage to moving parts. Any thoughts? Kevin Johnson University of Miami Diabetes Research Institute _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brett_connolly <@t> merck.com Fri Jun 14 15:21:52 2013 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Fri Jun 14 15:21:56 2013 Subject: [Histonet] RE: Cutting paraffin sections...on a cryostat? In-Reply-To: <59F7809368E0084C8AE6B38C93D735EB2FA3D609C3@MEDEXMB02.ad.med.miami.edu> References: <59F7809368E0084C8AE6B38C93D735EB2FA3D609C3@MEDEXMB02.ad.med.miami.edu> Message-ID: Sure it could be done - low throughput and very awkward though. So, no other lab at the university has a microtome that they would let a fellow researcher get some time on ??? Brett M. Connolly, Ph.D. Principal Scientist, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Johnson, Kevin Sent: Friday, June 14, 2013 4:09 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Cutting paraffin sections...on a cryostat? Hi, all. A bit of an odd question: a colleague knows of someone wanting to cut paraffin sections who has a cryostat, but no microtome. Since a cryostat's basically a microtome in a freezer chamber, I thought that it may be awkward, but theoretically doable once it was brought to room temp and dried out thoroughly. However, I wondered if lubricants formulated for the cold might become too thin for use at room temp, possibly causing damage to moving parts. Any thoughts? Kevin Johnson University of Miami Diabetes Research Institute _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From rjbuesa <@t> yahoo.com Fri Jun 14 15:51:34 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jun 14 15:51:38 2013 Subject: [Histonet] Cutting paraffin sections...on a cryostat? In-Reply-To: <59F7809368E0084C8AE6B38C93D735EB2FA3D609C3@MEDEXMB02.ad.med.miami.edu> References: <59F7809368E0084C8AE6B38C93D735EB2FA3D609C3@MEDEXMB02.ad.med.miami.edu> Message-ID: <1371243094.97073.YahooMailNeo@web163106.mail.bf1.yahoo.com> It is theoretically possible although very "odd" to say the last. Never mind the lubricants. The only thing is that the HT may end with a terrible back pain. Ren? J. From: "Johnson, Kevin" To: "'histonet@lists.utsouthwestern.edu'" Sent: Friday, June 14, 2013 4:09 PM Subject: [Histonet] Cutting paraffin sections...on a cryostat? Hi, all.? A bit of an odd question: a colleague knows of someone wanting to cut paraffin sections who has a cryostat, but no microtome. Since a cryostat's basically a microtome in a freezer chamber, I thought that it may be awkward, but theoretically doable once it was brought to room temp and dried out thoroughly. However, I wondered if lubricants formulated for the cold might become too thin for use at room temp, possibly causing damage to moving parts.? Any thoughts? Kevin Johnson University of Miami Diabetes Research Institute _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From latecor <@t> montevideo.com.uy Fri Jun 14 22:07:51 2013 From: latecor <@t> montevideo.com.uy (C.D.G.) Date: Fri Jun 14 22:08:00 2013 Subject: [Histonet] manual setup for Reichert-Jung 2050 needed Message-ID: <201306150007510574.0040B781@smtp.montevideo.com.uy> Hi all: i received a Reichert-Jung microtome 2050 model. I need instructions for its operation. The electronic panel displays "stop" illuminated and I dont know how to continue, as other buttons seems not to operate. Any help of people who has worked or know to operate this motorized microtome will be appreciated. My kind regards, Carlos Defeo Histotechnologist From tgenade <@t> gmail.com Sat Jun 15 06:20:57 2013 From: tgenade <@t> gmail.com (Tyrone Genade) Date: Sat Jun 15 06:21:08 2013 Subject: [Histonet] importing tissue blocks into the USA Message-ID: Hello, I have a box of tissue blocks which I want to take with me to my new lab in the USA. The tissue was fixed in Bouin's Fluid or Davidson's Fixative and then set in wax for sectioning. I have contacted US Customs who said this is an issue for the EPA. I contacted the EPA and they said that because this is for R&D there is an exception of some kind allowing me to import the blocks. I then asked them what forms I need to complete so that the blocks are cleared on arrival (i.e. Customs knows this is the EPA's business and the EPA knows its for R&D and I don't get fined or tossed in prison). The EPA then referred me to their website (or several pages on it) and have now three times not answered my direct questions of "is there a form" and "which form is it?" (I wonder if the person on the other end of the TSCA hotline knows themselves.) I can't figure out what the inventory ( http://www.epa.gov/opptintr/existingchemicals/pubs/tscainventory/howto.html) has to do with anything; and the TSCA Import & Export Requirements ( http://www.epa.gov/oppt/import-export/) also doesn't answer my questions (given above). Has anyone ever had to import samples for analysis and would you care to tell me what hoops I have to jump through and forms need to be filled in? The EPA does seem to go out of it way to be unclear and difficult. :-( Thanks -- Tyrone the Perplexed Department of Human Biology University of Cape Town From b427297 <@t> aol.com Sat Jun 15 15:17:20 2013 From: b427297 <@t> aol.com (Jackie O'Connor) Date: Sat Jun 15 15:17:25 2013 Subject: [Histonet] importing tissue blocks into the USA In-Reply-To: References: Message-ID: <8D0381C786987D7-17C8-D833@webmail-d140.sysops.aol.com> Are these human or animal tissues? If they are monkey or pig, you will have a problem. I import animal blocks routinely from Europe and Japan. Please contact me privately on Monday and I can give you more information once I am back in my office. Jackie O'Connor -----Original Message----- From: Tyrone Genade To: histonet Sent: Sat, Jun 15, 2013 6:22 am Subject: [Histonet] importing tissue blocks into the USA Hello, I have a box of tissue blocks which I want to take with me to my new lab in the USA. The tissue was fixed in Bouin's Fluid or Davidson's Fixative and then set in wax for sectioning. I have contacted US Customs who said this is an issue for the EPA. I contacted the EPA and they said that because this is for R&D there is an exception of some kind allowing me to import the blocks. I then asked them what forms I need to complete so that the blocks are cleared on arrival (i.e. Customs knows this is the EPA's business and the EPA knows its for R&D and I don't get fined or tossed in prison). The EPA then referred me to their website (or several pages on it) and have now three times not answered my direct questions of "is there a form" and "which form is it?" (I wonder if the person on the other end of the TSCA hotline knows themselves.) I can't figure out what the inventory ( http://www.epa.gov/opptintr/existingchemicals/pubs/tscainventory/howto.html) has to do with anything; and the TSCA Import & Export Requirements ( http://www.epa.gov/oppt/import-export/) also doesn't answer my questions (given above). Has anyone ever had to import samples for analysis and would you care to tell me what hoops I have to jump through and forms need to be filled in? The EPA does seem to go out of it way to be unclear and difficult. :-( Thanks -- Tyrone the Perplexed Department of Human Biology University of Cape Town _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From adesupo2002 <@t> hotmail.com Sat Jun 15 20:10:33 2013 From: adesupo2002 <@t> hotmail.com (ADESUPO ADESUYI) Date: Sat Jun 15 20:10:38 2013 Subject: [Histonet] Histology Lab Plan Message-ID: Hi, We are in the process of moving the histology lab from the present building to a new one. I will appreciate it if you guys in histo land could help me with the layout plan taking lean into consideration. I mean the plan to start from Accessioning , Gross, Tissue Processors,Embedding Center, Microtomy, Routine Stainer, Special Stainer, Ventana XT and Ultra. Thank you for your usual cooperation. Thanks, Adesupo From rennie1108 <@t> yahoo.com Sun Jun 16 10:23:49 2013 From: rennie1108 <@t> yahoo.com (Adrienne Anderson) Date: Sun Jun 16 10:23:57 2013 Subject: [Histonet] RE: Adrienne Anderson Message-ID: <1371396229.99679.YahooMailNeo@web125601.mail.ne1.yahoo.com> Hello! http://www.adicea.com/lrgo/vmc/azyir/ajxo.htm Adrienne Anderson vgzb From rjbuesa <@t> yahoo.com Sun Jun 16 10:33:27 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sun Jun 16 10:33:31 2013 Subject: [Histonet] Histology Lab Plan In-Reply-To: References: Message-ID: <1371396807.72763.YahooMailNeo@web163102.mail.bf1.yahoo.com> You know exactly what you want. Why don't you ask the person who is going to draw the plans for your new lab (we do not know the size of the layout you have) to include what you need. The flow you describe is OK, but do not try to put it in a continuous because it will not be adequate. Imagine like a "U"; start (as you point out) with accessioning, continue further some steps, turn around and end side by side where you started BUT finish not with a histology task, but in the area where the pathologists are that is where your slides are destined to end. Ren? J. From: ADESUPO ADESUYI To: "histonet@lists.utsouthwestern.edu" Sent: Saturday, June 15, 2013 9:10 PM Subject: [Histonet] Histology Lab Plan Hi, ? ? We are in the process of moving the histology lab from the present building to a new one. I will appreciate it if you guys in histo land could help me with the layout plan taking lean into consideration. I mean the plan to start from Accessioning , Gross, Tissue Processors,Embedding Center, Microtomy, Routine Stainer, Special Stainer, Ventana XT and Ultra. ? Thank you for your usual cooperation. ? Thanks, Adesupo ??? ??? ??? ? ??? ??? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mmooreht <@t> yahoo.com Sun Jun 16 10:42:11 2013 From: mmooreht <@t> yahoo.com (Michelle Moore) Date: Sun Jun 16 11:36:49 2013 Subject: [Histonet] RE: Michelle Moore Message-ID: <1371397331.24954.YahooMailNeo@web125101.mail.ne1.yahoo.com> http://montrealbarbell.com/nnlxwbn/cgia/pfstr/ucw.htm Best regards, Michelle Moore From Steven.Weston <@t> utas.edu.au Mon Jun 17 06:10:17 2013 From: Steven.Weston <@t> utas.edu.au (Steven Weston) Date: Mon Jun 17 06:10:31 2013 Subject: [Histonet] re 2050 microtome Message-ID: <7B808A2E6BDDBD4EA4FA6395FF7BED2B5E7E2402@MBXSBYN2.utas.ad.internal> This could be as simple as moving the specimen holder forward. It may be racked right into the microtome and therefore show as stopped. Try moving the holder forward and see if the stopped light goes off. Regards steve weston lab manager Breathe-Well CRE UTAS-SOM Message: 9 Date: Sat, 15 Jun 2013 00:07:51 -0300 From: "C.D.G." Subject: [Histonet] manual setup for Reichert-Jung 2050 needed To: histonet@lists.utsouthwestern.edu Cc: histonet@lists.utsouthwestern.edu Message-ID: <201306150007510574.0040B781@smtp.montevideo.com.uy> Content-Type: text/plain; charset="ISO-8859-1" Hi all: i received a Reichert-Jung microtome 2050 model. I need instructions for its operation. The electronic panel displays "stop" illuminated and I dont know how to continue, as other buttons seems not to operate. Any help of people who has worked or know to operate this motorized microtome will be appreciated. My kind regards, Carlos Defeo Histotechnologist From Sandra.Harrison3 <@t> va.gov Mon Jun 17 08:30:06 2013 From: Sandra.Harrison3 <@t> va.gov (Harrison, Sandra C.) Date: Mon Jun 17 08:30:40 2013 Subject: [Histonet] Cutting paraffin sections on a cryostat operated at room temperature? Nope. Message-ID: Ever tried turning the handle of the cryostat, when it's at room temperature? Cryostats are tooled & manufactured to operate at a low temperature. Since metal contracts at the low temperature, you'll find that you can't operate the microtome at the higher temperature. The handle will barely move. Sandy Harrison, HTL (ASCP) Histology Supervisor Minneapolis VAHCS 612-467-2449 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Johnson, Kevin Sent: Friday, June 14, 2013 3:09 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Cutting paraffin sections...on a cryostat? Hi, all. A bit of an odd question: a colleague knows of someone wanting to cut paraffin sections who has a cryostat, but no microtome. Since a cryostat's basically a microtome in a freezer chamber, I thought that it may be awkward, but theoretically doable once it was brought to room temp and dried out thoroughly. However, I wondered if lubricants formulated for the cold might become too thin for use at room temp, possibly causing damage to moving parts. Any thoughts? Kevin Johnson University of Miami Diabetes Research Institute _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ratliffjack <@t> hotmail.com Mon Jun 17 08:54:53 2013 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Mon Jun 17 08:54:59 2013 Subject: [Histonet] Paraffin processing native sheep ACL In-Reply-To: References: Message-ID: You might also consider using methyl salicylate instead of xylenes. Thanks to the help of Bob Skinner, I have achieved very nice results with native tendon. Generally speaking these MS steps will take a little longer, but you can monitor the progress very easily by watching for complete transparency of the tendon. You can then even develop a somewhat standardized protocol if you plan to process this type of tissue in the future. You even have a lot more flexibility with MS than xylenes as prolonged use in xylenes can make the tissue more hardened and brittle. Lastly, it is not generally recommended to put MS on the tissue processor, so I process to the final 100% EtOH, perform the MS exchanges by hand, transfer the tissues into a manual wax step to get rid of as much MS as possible and then finish with three (3) automated wax steps on the tissue processor. For my wax infiltration I use a 50:50 blend of TissuePrep from Fisher Scientific and EM400 from Leica. I then embed in 100% EM400. Best Regards, Jack Jack L RatliffOwner/Histologist, Ratliff Histology Consultants, LLCChairman, Hard Tissue Committee - National Society for Histotechnology > From: a.prior@tissueregenix.com > To: histonet@lists.utsouthwestern.edu > Date: Fri, 14 Jun 2013 07:45:47 +0000 > Subject: Re: [Histonet] Paraffin processing native sheep ACL > CC: > > Hi Liz, > > > > I inherited the following protocol for ACL samples. It works quite well, but times probably could be reduced - the optimization is on my to-do list. > > 70% Alcohol - 1 hour > > 90% alcohol - 1 hour > > 100% Alcohol -2 hours > > 100% alcohol - 3 hours > > 100% alcohol - 4 hours > > Xylene - 1.5 hours > > Xylene - 1.5 hours > > Xylene - 3 hours > > Wax - 3 hours > > Wax - 3 hours > > Wax - 4 hours > > I cut the sections at 8um so they hold together better. Takes a while for all the wrinkles to disappear when floating out on water-bath so be patient > > Hope this helps. > > > > Andrew > > > Andrew Prior > Histologist > Tissue Regenix Group > E-mail: a.prior@tissueregenix.com > Website: www.tissueregenix.com > > > > ------------------------------ > > Message: 3 > > Date: Wed, 12 Jun 2013 16:59:52 -0400 > > From: Elizabeth Ronan > > > Subject: [Histonet] Paraffin processing native sheep ACL > > To: histonet@lists.utsouthwestern.edu > > Message-ID: <51B8E148.3020102@umich.edu> > > Content-Type: text/plain; charset=ISO-8859-1; format=flowed > > > > Hello, > > > > I need to paraffin process native sheep anterior cruciate ligament (ACL) that has been fixed in 10% neutral buffered formalin for 7 days. I was wondering if anyone with more expertise on this subject could guide me with the best lengths of times in the alcohols, xylenes, and paraffin to fix ACL. I tried a 12 hour program but the sections crumbled in the middle and it appeared that the paraffin had not fully perfused the ligament. > > > > I have access to the following program, and can alter the lengths of the steps for as long as desired: > > > > Program: > > 70% > > 80% > > 95% > > 95% > > 100% > > 100% > > Xylene > > Xylene > > Paraffin > > Paraffin > > Paraffin > > > > Any advice is much appreciated. > > Thanks for your time, > > Liz > > > > The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited. If you received this in error, please contact the sender and delete the material from any computer. Although we routinely screen for viruses, addressees should check this e-mail and any attachment for viruses. We make no warranty as to absence of viruses in this e-mail or any attachments. > > Registered Office: The Biocentre, Innovation Way, Heslington, York, YO10 5NY > Registered No. 05969271 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jshelley <@t> sanfordburnham.org Mon Jun 17 09:30:03 2013 From: jshelley <@t> sanfordburnham.org (John Shelley) Date: Mon Jun 17 09:30:10 2013 Subject: [Histonet] Picro Sirius Red Stain Message-ID: Hi All, Hope all are having a great Monday!!!! I am trying to do a Picro Sirius Red stain and have looked at many protocols that seem to not use a particular reagent that is in a manufacturers kit. I was hoping if someone could enlighten me as to why this reagent would be used and if someone is using it at what strength is the PhosoMolybdic Acid concentration. Your help will be greatly appreciated!!!! Kind Regards! John J Shelley Research Specialist, Histology Core Facility From hmarlatt26 <@t> gmail.com Mon Jun 17 09:33:21 2013 From: hmarlatt26 <@t> gmail.com (Heather Marlatt) Date: Mon Jun 17 09:33:27 2013 Subject: [Histonet] Processing Guinea Pig Message-ID: Hello Histonet! I'm a long time reader first time poster. Does anyone have experience processing guinea pig tissues? I have been processing kidney and heart but it is consistently coming out mushy in the middle. The mouse tissue comes out fine even when processed on the same run. I had the tissue grossed in thinner (2.5mm) thinking that perhaps it was too thick but it didn't seem to help. Also, it has been fixed in 10%NBF for several days. I was just wondering if anyone else had similar problems with guinea pig? I appreciate in advance any advice or tips. Here is the protocol: Formalin 1hr 70% etOH 1hr 95% 1hr 100% 30min 100% 1hr 100% 1hr 100%1hr Clearify 1hr Clearify 1hr Clearify 1hr Paraffin 1hr paraffin 1hr All under pressure and heat only on the paraffin. Thanks Heather From algranth <@t> email.arizona.edu Mon Jun 17 10:28:30 2013 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Mon Jun 17 10:28:33 2013 Subject: [Histonet] Picro Sirius Red Stain In-Reply-To: References: Message-ID: <02DCE952-FD4D-432E-BB62-84AD17A507D6@email.arizona.edu> I do a Picro Sirius Red stain for collagen (got the protocol from Gayle Callis) and don't use Phosphomolybdic acid. My protocol is simple: one hour in PSR two rinses in acidified water rinse you can counterstain but my clients don't want a counterstain so I dehydrate, clear and coverslip. Boom, I'm done. The PSR stain is just Sirius Red F3B 0.5 gms and 500 ml of Sat. Picric Acid. Do not use a dye that is not CI 35780. The acidified water is 5ml Glacial Acetic Acid to 1L of DH2O Under polarized light the PSR stain is gorgeous! I love looking at the orange, yellow, pinks and green colors. Good thing because I do this stain all the time. Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 From dunatrsd <@t> sbcglobal.net Mon Jun 17 10:35:43 2013 From: dunatrsd <@t> sbcglobal.net (dusko trajkovic) Date: Mon Jun 17 10:35:48 2013 Subject: [Histonet] Cryostat Repair Service in San Diego In-Reply-To: <7B808A2E6BDDBD4EA4FA6395FF7BED2B5E7E2402@MBXSBYN2.utas.ad.internal> References: <7B808A2E6BDDBD4EA4FA6395FF7BED2B5E7E2402@MBXSBYN2.utas.ad.internal> Message-ID: <1371483343.29781.YahooMailNeo@web181703.mail.ne1.yahoo.com> Good Morning, Can anyone recomend a good, reliable cryostat repair service in the San diego area? thanks Dusko From avogaro <@t> science.unitn.it Mon Jun 17 10:35:44 2013 From: avogaro <@t> science.unitn.it (Laura Avogaro) Date: Mon Jun 17 10:35:52 2013 Subject: [Histonet] Picro Sirius Red Stain In-Reply-To: References: Message-ID: <3388.192.168.178.78.1371483344.squirrel@www.science.unitn.it> Dear John, I usually perform Picrosirius Red stain in cardiac tisse sections. I use phosphomolibic acid treatment in order to eliminate the cytoplasmic staining improving collagen/myoplasm contrast. Have a look at this paper: Picrosirius red staining of cardiac muscle following phosphomolybdic acid treatment Dolber PC, Spach MS Stain Technol. 1987 Jan;62(1):23-6. Good luck! Laura > Hi All, > > Hope all are having a great Monday!!!! > > I am trying to do a Picro Sirius Red stain and have looked at many > protocols that seem to not use a particular reagent that is in a > manufacturers kit. I was hoping if someone could enlighten me as to why > this reagent would be used and if someone is using it at what strength is > the PhosoMolybdic Acid concentration. > > Your help will be greatly appreciated!!!! > > Kind Regards! > > John J Shelley > Research Specialist, Histology Core Facility > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Laura Avogaro University of Trento Via delle Regole, 101 38123 Mattarello (TN) ? Italy Tel: +39 0461 283425 From Kari.Breal <@t> alexian.net Mon Jun 17 10:40:32 2013 From: Kari.Breal <@t> alexian.net (Breal, Kari) Date: Mon Jun 17 10:40:39 2013 Subject: [Histonet] ACIS CALIBRATION SLIDE SET Message-ID: Does anyone have an ACIS Calibration set they are not using and are willing to part with? One of my slides broke and I can't find a replacement anywhere. Thanks, Kari Breal Kari Breal, HT (ASCP) Histology Manager Alexian Brothers Health System ABMC-847-437-5500 ext. 5155 SAMC-847-843-2000 ext. 6818 kari.breal@alexian.net CONFIDENTIALITY NOTICE: This email message and any accompanying data or files is confidential and may contain privileged information intended only for the named recipient(s). If you are not the intended recipient(s), you are hereby notified that the dissemination, distribution, and or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at the email address above, delete this email from your computer, and destroy any copies in any form immediately. Receipt by anyone other than the named recipient(s) is not a waiver of any attorney-client, work product, or other applicable privilege. From algranth <@t> email.arizona.edu Mon Jun 17 10:50:09 2013 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Mon Jun 17 10:50:15 2013 Subject: [Histonet] Processing Guinea Pig In-Reply-To: References: Message-ID: Heather, Looking at your protocol for processing the kidney and heart tissue I can't figure out why it is mushy especially if you are putting it in the cassettes so thin and it has had a chance to fix well before processing. In fact, looking at your protocol I might think that your tissue might be dried out and need to sit in cold icy water before sectioning. At any rate, I would rinse well in running water after fixing and skip the formalin on the processor. I'd start in 70% alcohol, maybe two changes and move on up to 80%, 2- 95%'s and 3-100%'s. I'm not familiar with the clearing agent that you use. I use Clear Rite 3 and never have a problem - 2 changes of Clear Rite 3 and 4 paraffins. I don't know how many cassettes you are processing but make sure there is adequate room for the reagents on the processor to move around the cassettes and that you have a good ratio of the reagents to the tissue and that the reagents are fresh. If you can increase the agitation in some of the dehydration steps it might help. Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 From cheastys <@t> svm.vetmed.wisc.edu Mon Jun 17 12:23:29 2013 From: cheastys <@t> svm.vetmed.wisc.edu (Sandra Cheasty) Date: Mon Jun 17 12:23:07 2013 Subject: [Histonet] Parts for Sakura Tissue Tek 5 Embedding Center Message-ID: Hello all you Histologists, Does anyone know of a used Histology equipment company that sells Sakura parts? We have a fairly new embedding center, (but past warranty), that needs a single heater unit. Sakura only sells the entire dispenser unit for nearly $8,000. I don't mean to offend any Sakura fans, but I don't think that's right. Thanks, Sandy Histology & Necropsy Supervisor UW-Madison From kecslide <@t> yahoo.com Mon Jun 17 12:23:16 2013 From: kecslide <@t> yahoo.com (kristy castillo) Date: Mon Jun 17 12:23:20 2013 Subject: [Histonet] Histology position Message-ID: <1371489796.32681.YahooMailNeo@web141105.mail.bf1.yahoo.com> Hi Histonetters, ? I am moving back to Phoenix, Arizona and I am looking for a Histology position.?? I have over 17 years experience?in the Histology field.? I am willing to?work full time and even part time.? Please feel free to contact me at kecslide@yahoo.com? I hope to hear from you soon.? Thank you for your time and consideration.? Kristy From tmoore9k <@t> gmail.com Mon Jun 17 16:10:51 2013 From: tmoore9k <@t> gmail.com (Teresa Moore) Date: Mon Jun 17 16:10:54 2013 Subject: [Histonet] Blade Rationing Message-ID: I work in a hospital, there are three of us on this particular shift and we cut approx. 200 blocks, give or take a few. Our histo lab manager is telling us we should only be using one pack of blades (50 per pack) a month. I'm wondering what other techs think of this especially lab managers and supervisors. tmoore9k@gmail.com From c.tague <@t> Pathologyarts.com Mon Jun 17 16:16:04 2013 From: c.tague <@t> Pathologyarts.com (Curt) Date: Mon Jun 17 16:16:08 2013 Subject: [Histonet] Blade Rationing In-Reply-To: References: Message-ID: <9C8F910F72893643B3C3793C3D67132B013A2BF5@PATHOLOGYSERVER.pathologyarts.local> That's penny pinching right there. I'd say fine, you tell me when to change blades and then put through the crap slides they get. Just avoid the calculi and staples at all costs... sometimes these number crunchers don't think. Curt -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Teresa Moore Sent: Monday, June 17, 2013 2:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Blade Rationing I work in a hospital, there are three of us on this particular shift and we cut approx. 200 blocks, give or take a few. Our histo lab manager is telling us we should only be using one pack of blades (50 per pack) a month. I'm wondering what other techs think of this especially lab managers and supervisors. tmoore9k@gmail.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mucram11 <@t> comcast.net Mon Jun 17 16:17:49 2013 From: mucram11 <@t> comcast.net (Pam Marcum) Date: Mon Jun 17 16:17:58 2013 Subject: [Histonet] Blade Rationing In-Reply-To: Message-ID: <189308733.318237.1371503869187.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Sorry, I tell my techs a knife is cheaper than a complaint from the pathologists so, please don't abuse.? H owever; don't hesitate to use what you need.? As teh old saying oges for us "A happy Patholgist is a happy life"!!? A dull is bad for sectioning and is dangerous overall.? I can't imagine telling my Histologist to cut corners on knives and they know me well enough that they would think I had lost my mind!!? So many other places to cut cost and not affect section quality. Pam Marcum Histology Supervisor UAMS ----- Original Message ----- From: "Teresa Moore" To: histonet@lists.utsouthwestern.edu Sent: Monday, June 17, 2013 4:10:51 PM Subject: [Histonet] Blade Rationing I work in a hospital, there are three of us on this particular shift and we cut approx. 200 blocks, give or take a few. ?Our histo lab manager is telling us we should only be using one pack of blades (50 per pack) a month. ?I'm wondering what other techs think of this especially lab managers and supervisors. tmoore9k@gmail.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek <@t> digestivespecialists.com Mon Jun 17 16:48:35 2013 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Mon Jun 17 16:48:41 2013 Subject: [Histonet] Blade Rationing In-Reply-To: References: Message-ID: <3F0FD258-D403-4E3D-A113-301B0285EA84@digestivespecialists.com> >From a managers point of view, whoin my opinion that is a poor way to try to cut expenses. It will only lead to recuts and possible loss of important tissue. For the techs to understand the necessity to conserve is important but the tech needs to use their discretion as to when a blade needs changing. Sent from my iPhone On Jun 17, 2013, at 5:08 PM, "Teresa Moore" wrote: > I work in a hospital, there are three of us on this particular shift and we > cut approx. 200 blocks, give or take a few. Our histo lab manager is > telling us we should only be using one pack of blades (50 per pack) a > month. I'm wondering what other techs think of this especially lab > managers and supervisors. > > tmoore9k@gmail.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Vickroy.Jim <@t> mhsil.com Mon Jun 17 17:09:53 2013 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Mon Jun 17 17:09:59 2013 Subject: [Histonet] HT and HTL Job Descriptions Message-ID: We are in the process of updating job descriptions and want to use this opportunity to see how other organizations are handling the difference in job descriptions between HT's and HTL's. Obviously if you have a different pay schedule for the two positions you need to clearly delineate the job descriptions and specify the increased responsibilities of staff that are HTL's. I am interested in how other institutions have handled this issue. In the past we have had the same job description for HT and HTL and have also not had a difference in pay schedules. I know that some places do not separate the two positions. Any information that you could share would be helpful as we look into this project. Thanks James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From Cynthia_Brundige <@t> ssmhc.com Mon Jun 17 17:21:22 2013 From: Cynthia_Brundige <@t> ssmhc.com (Brundige, Cynthia) Date: Mon Jun 17 17:21:29 2013 Subject: [Histonet] RE: HT and HTL Job Descriptions In-Reply-To: References: Message-ID: <3651CE1AFD7C6C4495776542DCE5AD1E0FF471@s928-apexm01.ds.ad.ssmhc.com> St. Anthony Hospital, located in Oklahoma City, Oklahoma currently has an excellent opportunities for experienced Histologic Technicians. To work for our organization requires Certification as an HT or HLT by the American Society of Clinical Pathologists (ASCP) - or - other nationally recognized certifying agency acceptable to the Laboratory Director - or - experience acceptable to the Laboratory Director. Two years of previous histology experience required. Outstanding benefits package, competitive pay and generous paid time off. For consideration, please apply online at www.saintsok.com, Ad # 17929, or contact Renee Watley for additional information at (405) 272-6016; renee_watley@ssmhc.com. Cynthia L. Brundige SSM Health Care of Oklahoma Regional Vice President-Human Resources 1000 N. Lee Street, P.O. Box 205 Oklahoma City, Oklahoma 73101 cynthia_brundige@ssmhc.com -----Original Message----- ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ Confidentiality Notice: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. From Timothy.Morken <@t> ucsfmedctr.org Mon Jun 17 17:31:05 2013 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Mon Jun 17 17:34:20 2013 Subject: [Histonet] RE: HT and HTL Job Descriptions In-Reply-To: References: Message-ID: <761E2B5697F795489C8710BCC72141FF083DF0@ex07.net.ucsf.edu> We have Histotechnologst 1, 2, 3, and Lead, Supervisor: We don't have a requirement that they have their HT or HTL certification, but do need specific experience in a histology lab. All require bachelor's degree with educational qualifications to sit for HT or HTL exam. Most have their HT or HTL. A few new people do not...yet. 1 - entry level bench tech, education equiv to one year experience ,ie some lab work. 2 - having one year histo lab experience 3 - having 4 years experience (Senior tech), Lead - having 5 years experience including all aspects of the lab. Supervisor, 10 years experience, general supervision and administration. Levels 1 and 2 are general bench techs rotating to all areas of the lab Level 3 is Senior tech who can be made responsible for overseeing one are of the lab, not to work there all the time, but be responsible for making sure everything is ship shape and people have what they need, do validations of stains and equipment. They also can take charge at the times a Lead or Supervisor is not around (ie, early AM or late PM) Lead Tech does more admin work (in addition to some bench work), troubleshooting, oversees procedure validation, workflow scheduling on the fly, generally keeps the lab running. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Monday, June 17, 2013 3:10 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HT and HTL Job Descriptions We are in the process of updating job descriptions and want to use this opportunity to see how other organizations are handling the difference in job descriptions between HT's and HTL's. Obviously if you have a different pay schedule for the two positions you need to clearly delineate the job descriptions and specify the increased responsibilities of staff that are HTL's. I am interested in how other institutions have handled this issue. In the past we have had the same job description for HT and HTL and have also not had a difference in pay schedules. I know that some places do not separate the two positions. Any information that you could share would be helpful as we look into this project. Thanks James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Susan.Walzer <@t> HCAHealthcare.com Tue Jun 18 02:07:02 2013 From: Susan.Walzer <@t> HCAHealthcare.com (Susan.Walzer@HCAHealthcare.com) Date: Tue Jun 18 02:07:30 2013 Subject: [Histonet] Blade Rationing In-Reply-To: <3F0FD258-D403-4E3D-A113-301B0285EA84@digestivespecialists.com> References: <3F0FD258-D403-4E3D-A113-301B0285EA84@digestivespecialists.com> Message-ID: <4BF03F5404EBDE409AF9232DA74B9DED2FA0A08080@FWDCWPMSGCMS09.hca.corpad.net> You need to tell your manager that you cannot do your job without proper tools. Only the tech cutting knows how many blades he or she needs to cut a days work. These micro managers need to do some bench work and get a reality check. Unbelievable! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Monday, June 17, 2013 5:49 PM To: Teresa Moore Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Blade Rationing >From a managers point of view, whoin my opinion that is a poor way to try to cut expenses. It will only lead to recuts and possible loss of important tissue. For the techs to understand the necessity to conserve is important but the tech needs to use their discretion as to when a blade needs changing. Sent from my iPhone On Jun 17, 2013, at 5:08 PM, "Teresa Moore" wrote: > I work in a hospital, there are three of us on this particular shift > and we cut approx. 200 blocks, give or take a few. Our histo lab > manager is telling us we should only be using one pack of blades (50 > per pack) a month. I'm wondering what other techs think of this > especially lab managers and supervisors. > > tmoore9k@gmail.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tpodawiltz <@t> lrgh.org Tue Jun 18 04:13:57 2013 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Tue Jun 18 04:14:05 2013 Subject: [Histonet] Blade Rationing In-Reply-To: References: Message-ID: <38667E7FB77ECD4E91BFAEB8D98638632500C1DD2D@LRGHEXVS1.practice.lrgh.org> Is your manager a Tech? Sure does not sound like one. Anyone that is willing to compromise the quality of diagnostic slides to save a dollar should not be in a management position. Tom Podawiltz HT (ASCP) Histology Section Head/Laboratory Safety Officer. LRGHealthcare Laconia, NH 03246 603-524-3211 ext: 3220 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Teresa Moore Sent: Monday, June 17, 2013 5:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Blade Rationing I work in a hospital, there are three of us on this particular shift and we cut approx. 200 blocks, give or take a few. Our histo lab manager is telling us we should only be using one pack of blades (50 per pack) a month. I'm wondering what other techs think of this especially lab managers and supervisors. tmoore9k@gmail.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From ree3 <@t> leicester.ac.uk Tue Jun 18 04:25:02 2013 From: ree3 <@t> leicester.ac.uk (Edwards, Richard E.) Date: Tue Jun 18 04:25:10 2013 Subject: [Histonet] Blade Rationing In-Reply-To: References: Message-ID: <7722595275A4DD4FA225B92CDBF174A101B0CE99604B@EXC-MBX3.cfs.le.ac.uk> What total rubbish, what planet is this manager from??? Good luck.................. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Teresa Moore Sent: 17 June 2013 22:11 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Blade Rationing I work in a hospital, there are three of us on this particular shift and we cut approx. 200 blocks, give or take a few. Our histo lab manager is telling us we should only be using one pack of blades (50 per pack) a month. I'm wondering what other techs think of this especially lab managers and supervisors. tmoore9k@gmail.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Tue Jun 18 07:14:00 2013 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Tue Jun 18 07:14:06 2013 Subject: [Histonet] RE: HT and HTL Job Descriptions In-Reply-To: <761E2B5697F795489C8710BCC72141FF083DF0@ex07.net.ucsf.edu> References: , <761E2B5697F795489C8710BCC72141FF083DF0@ex07.net.ucsf.edu> Message-ID: Pleased to see that you have many options for employees to pursue and fit in with different skill and education levels. I definitely prefer new hires that are at least exam eligible. In my lab situation it is just not good for the workflow to have work around their ability to do or not do different tasks. However, I know that people come into histology by various paths, so I am willing to train and support anyone who is seriously pursuing certification. This may have to be looked at differently when you are working with already employees ( not new hires) that are in various situations with regards to their education, training and experience. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: Timothy.Morken@ucsfmedctr.org > To: Vickroy.Jim@mhsil.com; histonet@lists.utsouthwestern.edu > Date: Mon, 17 Jun 2013 22:31:05 +0000 > CC: > Subject: [Histonet] RE: HT and HTL Job Descriptions > > We have Histotechnologst 1, 2, 3, and Lead, Supervisor: We don't have a requirement that they have their HT or HTL certification, but do need specific experience in a histology lab. > All require bachelor's degree with educational qualifications to sit for HT or HTL exam. Most have their HT or HTL. A few new people do not...yet. > 1 - entry level bench tech, education equiv to one year experience ,ie some lab work. > 2 - having one year histo lab experience > 3 - having 4 years experience (Senior tech), > Lead - having 5 years experience including all aspects of the lab. > Supervisor, 10 years experience, general supervision and administration. > > Levels 1 and 2 are general bench techs rotating to all areas of the lab > Level 3 is Senior tech who can be made responsible for overseeing one are of the lab, not to work there all the time, but be responsible for making sure everything is ship shape and people have what they need, do validations of stains and equipment. They also can take charge at the times a Lead or Supervisor is not around (ie, early AM or late PM) > Lead Tech does more admin work (in addition to some bench work), troubleshooting, oversees procedure validation, workflow scheduling on the fly, generally keeps the lab running. > > > Tim Morken > Supervisor, Electron Microscopy/Neuromuscular Special Studies > Department of Pathology > UC San Francisco Medical Center > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim > Sent: Monday, June 17, 2013 3:10 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] HT and HTL Job Descriptions > > > We are in the process of updating job descriptions and want to use this opportunity to see how other organizations are handling the difference in job descriptions between HT's and HTL's. Obviously if you have a different pay schedule for the two positions you need to clearly delineate the job descriptions and specify the increased responsibilities of staff that are HTL's. > > I am interested in how other institutions have handled this issue. In the past we have had the same job description for HT and HTL and have also not had a difference in pay schedules. I know that some places do not separate the two positions. Any information that you could share would be helpful as we look into this project. Thanks > > James Vickroy BS, HT(ASCP) > > Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center > 217-788-4046 > > > ________________________________ > This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Tue Jun 18 07:19:37 2013 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Tue Jun 18 07:19:42 2013 Subject: [Histonet] Blade Rationing In-Reply-To: <4BF03F5404EBDE409AF9232DA74B9DED2FA0A08080@FWDCWPMSGCMS09.hca.corpad.net> References: , <3F0FD258-D403-4E3D-A113-301B0285EA84@digestivespecialists.com>, <4BF03F5404EBDE409AF9232DA74B9DED2FA0A08080@FWDCWPMSGCMS09.hca.corpad.net> Message-ID: Certainly techs should be conscious of not wasting supplies- but I have never known this to be an issue. If the goal is to reduce costs,? Rationing blades has to be one of the worst and least effective means to achieve that. I can think of so many other ways to reduce waste(cost), that will have a greater positive impact on the bottom line. The re-work alone might become ridiculous. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: Susan.Walzer@HCAHealthcare.com > To: lblazek@digestivespecialists.com; tmoore9k@gmail.com > Date: Tue, 18 Jun 2013 02:07:02 -0500 > Subject: RE: [Histonet] Blade Rationing > CC: histonet@lists.utsouthwestern.edu > > You need to tell your manager that you cannot do your job without proper tools. Only the tech cutting knows how many blades he or she needs to cut a days work. These micro managers need to do some bench work and get a reality check. Unbelievable! > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda > Sent: Monday, June 17, 2013 5:49 PM > To: Teresa Moore > Cc: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Blade Rationing > > >From a managers point of view, whoin my opinion that is a poor way to try to cut expenses. It will only lead to recuts and possible loss of important tissue. For the techs to understand the necessity to conserve is important but the tech needs to use their discretion as to when a blade needs changing. > > Sent from my iPhone > > On Jun 17, 2013, at 5:08 PM, "Teresa Moore" wrote: > > > I work in a hospital, there are three of us on this particular shift > > and we cut approx. 200 blocks, give or take a few. Our histo lab > > manager is telling us we should only be using one pack of blades (50 > > per pack) a month. I'm wondering what other techs think of this > > especially lab managers and supervisors. > > > > tmoore9k@gmail.com > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Karen.Heckford <@t> DignityHealth.org Tue Jun 18 07:28:29 2013 From: Karen.Heckford <@t> DignityHealth.org (Heckford, Karen - SMMC-SF) Date: Tue Jun 18 07:28:35 2013 Subject: [Histonet] Blade Rationing In-Reply-To: References: Message-ID: Wow!!! It is pretty obvious this person has never worked as a HT. There are somethings you can cut corners but somethings you cannot. Do they want unreadable slides. I would without hesitation say to this manager the reason why this is not a good idea and would he want substandard slides if it was his tissue or someone he loves. Augh!!!! Stuff like this just makes me angry. Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Teresa Moore Sent: Monday, June 17, 2013 2:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Blade Rationing I work in a hospital, there are three of us on this particular shift and we cut approx. 200 blocks, give or take a few. Our histo lab manager is telling us we should only be using one pack of blades (50 per pack) a month. I'm wondering what other techs think of this especially lab managers and supervisors. tmoore9k@gmail.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DKBoyd <@t> chs.net Tue Jun 18 07:42:59 2013 From: DKBoyd <@t> chs.net (Boyd, Debbie M) Date: Tue Jun 18 07:43:10 2013 Subject: [Histonet] Blade Rationing In-Reply-To: References: Message-ID: <7EAFE982E328304DA6CE2B677BB76246794E31C4@TN001WEXMBX12.US.chs.net> The price of a few blades is nothing compared to a law suit for intentional neglect and endangerment to patient care. Not to mention some blades are "bad" when they come out of the box. This is a very good example of "pound foolish and penny wise". Only the tech cutting knows when they need to change a blade. I encourage my techs to change blades often. It's all about getting good sections. When our slides go out to other institutes (for consults) it is a reflection on our work. Not to mention the cost for recuts (if the tissues hasn't been lost). Tech time is expensive too. One must weigh the whole situation in terms of cost. I always tell my techs to think of each specimen as one of their family member's. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Teresa Moore Sent: Monday, June 17, 2013 5:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Blade Rationing I work in a hospital, there are three of us on this particular shift and we cut approx. 200 blocks, give or take a few. Our histo lab manager is telling us we should only be using one pack of blades (50 per pack) a month. I'm wondering what other techs think of this especially lab managers and supervisors. tmoore9k@gmail.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From jbro22 <@t> lsuhsc.edu Tue Jun 18 08:45:21 2013 From: jbro22 <@t> lsuhsc.edu (Browning, Jeffrey A.) Date: Tue Jun 18 08:45:30 2013 Subject: [Histonet] Lab Technician - Gross room/Autopsy (Shreveport, LA) Message-ID: <2E3D5719BC42374E982EF0AD611F23628A49AF64@SH-EXCHMB3.master.lsuhsc.edu> Greetings, We currently have an opening for a Laboratory Technician to assist with gross room and autopsy procedures (Shreveport, LA). Please contact me for full job description and link to application website. Thanks! Jeff Browning, HTL(ASCP) Technical Director, Anatomic Pathology Department of Pathology LSU Health Science Center - Shreveport 1501 Kings Highway Shreveport, LA 71103 (318) 675-5872 jbro22@lsuhsc.edu From Thomas.Jasper <@t> deaconess.com Tue Jun 18 08:51:32 2013 From: Thomas.Jasper <@t> deaconess.com (Thomas Jasper) Date: Tue Jun 18 08:51:38 2013 Subject: [Histonet] Blade Rationing In-Reply-To: References: Message-ID: Teresa, I concur with the all the responses. It seems your lab manager is not grasping the technical reality you must work under. If you are being asked to save money on blades why not try some different brands or negotiate some better pricing? That is something the lab manager can work on. Also, I would think you are doing your best optimize the use of each blade. You should be able to get 3 good cutting areas per blade before they're spent. Another consideration is having some blades for facing in only. I'm guessing the manager is being pressured to cut cost. I would look in other areas and at other items. Blades are of too critical importance to mess around with much. Good luck, Tom Jasper Thomas Jasper HT (ASCP) BAS AP Supervisor Deaconess Hospital Evansville, IN -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Teresa Moore Sent: Monday, June 17, 2013 4:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Blade Rationing I work in a hospital, there are three of us on this particular shift and we cut approx. 200 blocks, give or take a few. Our histo lab manager is telling us we should only be using one pack of blades (50 per pack) a month. I'm wondering what other techs think of this especially lab managers and supervisors. tmoore9k@gmail.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Deaconess Health System. If you have received this email in error please notify the originator of the message. From pam <@t> dlcjax.com Tue Jun 18 08:52:34 2013 From: pam <@t> dlcjax.com (pam@dlcjax.com) Date: Tue Jun 18 08:52:39 2013 Subject: [Histonet] (no subject) Message-ID: <249931259.65611.1371563554576.JavaMail.mail@webmail13> Please remove me from the mailing list. Thanks Pam Mathews, CDC Dermatology and Laser Center Orange Park, Florida 32073 Office Manager 904-276-4500 Office 904-276-4160 Fax 904-945-6845 Cell From rjbuesa <@t> yahoo.com Tue Jun 18 08:55:10 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jun 18 08:55:13 2013 Subject: [Histonet] Blade Rationing In-Reply-To: References: Message-ID: <1371563710.85433.YahooMailNeo@web163102.mail.bf1.yahoo.com> At 200 blocks/day x 5 days/week x 4 weeks/month = 4000 blocks/month which means that the 3 of you will have to use 1 blade every 80 blocks including trimming and sectioning which is ABSOLUTELY RIDICULOUS! The cost of blades, especially the better ones, are going up and you can save by using one blade to trim and another to?make the final section, but at the rate your manager wants the quality will be compromised. The "norm" (if there is a norm at all) is that a histotech will probably change blades every 5 to 10 blocks if the infiltration is good and there are no decals involved in the process. Lets assume that you can hold to 1 blade every 10 blocks, that will mean that during 1 month you will use 400 blades = 8 blades boxes. Find out how many you are actually using now and you will have an idea of your present blades usage. Additionally dull blades not only compromise the quality of the sections but also reduce sectioning productivity and what you may be saving in blades are going to increase in histotech time and total section production costs. Ren? J. From: Teresa Moore To: histonet@lists.utsouthwestern.edu Sent: Monday, June 17, 2013 5:10 PM Subject: [Histonet] Blade Rationing I work in a hospital, there are three of us on this particular shift and we cut approx. 200 blocks, give or take a few.? Our histo lab manager is telling us we should only be using one pack of blades (50 per pack) a month.? I'm wondering what other techs think of this especially lab managers and supervisors. tmoore9k@gmail.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 <@t> earthlink.net Tue Jun 18 09:01:02 2013 From: relia1 <@t> earthlink.net (Pam Barker) Date: Tue Jun 18 09:01:04 2013 Subject: [Histonet] RELIA Hot Job Alert 6-17-2013 Exciting and Immediate Opportunities in TX, NC, CA, MA, GA, NJ, TN, and VA Message-ID: <009301ce6c2c$44f4fc70$cedef550$@earthlink.net> Hello Histonetters!!!, I hope you are having a great week. I wanted to send a quick note to tell you about the positions that I am working on and am most excited about. Why am I excited about these positions? Because each of these clients was asked if I had a histotech for you today would you be ready to interview and hire. Every One of these clients said YES!!! All of these positions are full time and permanent!! My clients offer excellent compensation, benefits and in most cases either relocation or a sign-on bonus. Here are the openings I am working on from these clients!! HISTOLOGY MANAGEMENT/SUPERVISORS Pathology Manager Vet/Research - Boston, MA Histology Supervisor - Atlanta, GA Lead Histotechnologist - Long Beach, CA HISTOTECHNICIAN/HISTOTECHNOLOGIST ***ASCP HT/HTL and 2 years exper required for all of these positions.*** Paterson, NJ-Night shift CLIA qual to gross Charlotte, NC-Night shift strong cutting and embedding Chattanooga, TN-Night shift Grossing Histotech CLIA Qual to gross Nashville, TN -Mid shift strong cutting embedding and staining Harrisonburg, VA - dayshift, IHC, CLIA Qual to gross and mentoring skills Atlanta, GA - Days Dermpath exper. Required Tyler, TX - Days strong routine histology Augusta, GA - Days routine histology Modesto, CA - IHC/strong cutter Ridgecrest, CA - Lead Histotech days. If you think you or someone you know might be interested in any of these opportunities or would like to talk about a job search in another area, please contact me. If I place someone you refer You will earn a referral fee. I can be reached toll free at the office at 866-607-3542 or relia1@earthlink.net or you can always catch me on cell via call or text at 407-353-5070. Thanks-Pam Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From ewj <@t> pigsqq.org Tue Jun 18 09:20:10 2013 From: ewj <@t> pigsqq.org (=?UTF-8?B?IkUuIFdheW5lIEpvaG5zb24g5pyx56iz5qOuIg==?=) Date: Tue Jun 18 09:20:32 2013 Subject: [Histonet] Blade Rationing In-Reply-To: <1371563710.85433.YahooMailNeo@web163102.mail.bf1.yahoo.com> References: <1371563710.85433.YahooMailNeo@web163102.mail.bf1.yahoo.com> Message-ID: <51C06C9A.5080205@pigsqq.org> A stingy person is called an Iron Rooster (tie gong ji) in Chinese. An iron rooster won't turn loose of even one feather. The scratches that appear on slides cut with blades that should have been changed --we call them iron rooster tracks. Sort of a pun since many people think Chinese characters look like chicken tracks. We don't worry about what the blades cost. Our clients demand good answers, and we send pictures of the lesions with our reports, so we need good results. On 3:59, Rene J Buesa wrote: > At 200 blocks/day x 5 days/week x 4 weeks/month = 4000 blocks/month which means that the 3 of you will have to use 1 blade every 80 blocks including trimming and sectioning which is ABSOLUTELY RIDICULOUS! > The cost of blades, especially the better ones, are going up and you can save by using one blade to trim and another to make the final section, but at the rate your manager wants the quality will be compromised. > The "norm" (if there is a norm at all) is that a histotech will probably change blades every 5 to 10 blocks if the infiltration is good and there are no decals involved in the process. > Lets assume that you can hold to 1 blade every 10 blocks, that will mean that during 1 month you will use 400 blades = blades boxes. > Find out how many you are actually using now and you will have an idea of your present blades usage. > Additionally dull blades not only compromise the quality of the sections but also reduce sectioning productivity and what you may be saving in blades are going to increase in histotech time and total section production costs. > Ren? J. > > From: Teresa Moore > To: histonet@lists.utsouthwestern.edu > Sent: Monday, June 17, 2013 5:10 PM > Subject: [Histonet] Blade Rationing > > > I work in a hospital, there are three of us on this particular shift and we > cut approx. 200 blocks, give or take a few. Our histo lab manager is > telling us we should only be using one pack of blades (50 per pack) a > month. I'm wondering what other techs think of this especially lab > managers and supervisors. > > tmoore9k@gmail.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From TNMayer <@t> mdanderson.org Tue Jun 18 09:46:53 2013 From: TNMayer <@t> mdanderson.org (Mayer,Toysha N) Date: Tue Jun 18 09:46:59 2013 Subject: [Histonet] New York Licensure Message-ID: <47E9B2C01DDDD94881EACD2DC44EBC880161D07C@D1PWPEXMBX05.mdanderson.edu> Good morning, This question is for those in the New York state area: What is needed for an HTL to become licensed to work in New York state. I have two students who are moving to the area after graduation (August), and will be eligible to sit for the ASCP HTL. The problem is the license requirements are listing HT as the qualifying certification. One student has contacted a recruiter and the state licensure agency and still is not sure what to do. The state society said that since there are no HTL programs, a HT exam would have to be passed. Huh??? The students are moving from Texas to New York state, and will hold a BS HTL, eligibility date for BOC of Aug 15. Any help would be appreciated. Toysha N. Mayer, MBA, HT (ASCP) Instructor, Education Coordinator Program in Histotechnology School of Health Professions MD Anderson Cancer Center (713) 563-3481 tnmayer@mdanderson.org From asmith <@t> mail.barry.edu Tue Jun 18 09:53:22 2013 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Tue Jun 18 09:53:35 2013 Subject: [Histonet] RE:Blade Rationing Message-ID: I run an academic lab on a very tight budget. A paper towel used to dry washed hands is used again. Outdated dye solutions are adsorbed onto a small pile of old paper towels to save on waste disposal costs. (A quarter-pound of solid waste costs less to dispose of than 2 liters of aqueous liquid waste.) Disposable pipettes are washed and reused until the numbers wear off. I make up Vector's ImmPact SG 1.7 ml a time, store it in the fridge, and use it all week. I don't save on microtome blades. Dull blades leave holes in 4 micron sections. Sections cut with a dull blade have the annoying habit of exploding on the water bath. Dull blades tease out collagen fibers and drape them over the cells I'm trying to study. When a blade is dull it goes into the sharps box. -Allen A. Smith, Ph.D. Barry University School of Podiatric Medicine Miami Shores, FL From Timothy.Morken <@t> ucsfmedctr.org Tue Jun 18 09:54:34 2013 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Tue Jun 18 09:57:54 2013 Subject: [Histonet] RE: HT and HTL Job Descriptions In-Reply-To: References: , <761E2B5697F795489C8710BCC72141FF083DF0@ex07.net.ucsf.edu> Message-ID: <761E2B5697F795489C8710BCC72141FF083EE9@ex07.net.ucsf.edu> Joelle, We have a situation here at UC San Francisco that is almost ideal concerning personnel. We have a largish lab - 13 techs in histo alone (Kaiser across the bay has over 40, so we feel large-ish compared to them!), 4 in EM, 8 in grossing, so people have an opportunity for variety. Our campus is specifically a medical/nursing/dental/psychiatry school and Medical Center, and has a huge research center. Many of our techs started out after college in the research center where they learned how to do basic bench work of all kinds and picked up histology skills along the way. The combination of research exposure and practical bench work makes for good techs. That helps a lot with filling spots that open. Fortunately lots of people want to live in the SF Bay Area, even though the cost of living is high, so we always have good applicants. Our medical staff is excellent and very, very appreciative of the techs working in the lab. That makes for a good working relationship for everyone. On top of all we pay very well! Tim From: joelle weaver [mailto:joelleweaver@hotmail.com] Sent: Tuesday, June 18, 2013 5:14 AM To: Morken, Timothy; Vickroy, Jim; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: HT and HTL Job Descriptions Pleased to see that you have many options for employees to pursue and fit in with different skill and education levels. I definitely prefer new hires that are at least exam eligible. In my lab situation it is just not good for the workflow to have work around their ability to do or not do different tasks. However, I know that people come into histology by various paths, so I am willing to train and support anyone who is seriously pursuing certification. This may have to be looked at differently when you are working with already employees ( not new hires) that are in various situations with regards to their education, training and experience. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: Timothy.Morken@ucsfmedctr.org > To: Vickroy.Jim@mhsil.com; histonet@lists.utsouthwestern.edu > Date: Mon, 17 Jun 2013 22:31:05 +0000 > CC: > Subject: [Histonet] RE: HT and HTL Job Descriptions > > We have Histotechnologst 1, 2, 3, and Lead, Supervisor: We don't have a requirement that they have their HT or HTL certification, but do need specific experience in a histology lab. > All require bachelor's degree with educational qualifications to sit for HT or HTL exam. Most have their HT or HTL. A few new people do not...yet. > 1 - entry level bench tech, education equiv to one year experience ,ie some lab work. > 2 - having one year histo lab experience > 3 - having 4 years experience (Senior tech), > Lead - having 5 years experience including all aspects of the lab. > Supervisor, 10 years experience, general supervision and administration. > > Levels 1 and 2 are general bench techs rotating to all areas of the lab > Level 3 is Senior tech who can be made responsible for overseeing one are of the lab, not to work there all the time, but be responsible for making sure everything is ship shape and people have what they need, do validations of stains and equipment. They also can take charge at the times a Lead or Supervisor is not around (ie, early AM or late PM) > Lead Tech does more admin work (in addition to some bench work), troubleshooting, oversees procedure validation, workflow scheduling on the fly, generally keeps the lab running. > > > Tim Morken > Supervisor, Electron Microscopy/Neuromuscular Special Studies > Department of Pathology > UC San Francisco Medical Center > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim > Sent: Monday, June 17, 2013 3:10 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] HT and HTL Job Descriptions > > > We are in the process of updating job descriptions and want to use this opportunity to see how other organizations are handling the difference in job descriptions between HT's and HTL's. Obviously if you have a different pay schedule for the two positions you need to clearly delineate the job descriptions and specify the increased responsibilities of staff that are HTL's. > > I am interested in how other institutions have handled this issue. In the past we have had the same job description for HT and HTL and have also not had a difference in pay schedules. I know that some places do not separate the two positions. Any information that you could share would be helpful as we look into this project. Thanks > > James Vickroy BS, HT(ASCP) > > Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center > 217-788-4046 > > > ________________________________ > This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Tue Jun 18 10:00:01 2013 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Tue Jun 18 10:03:22 2013 Subject: [Histonet] Blade Rationing In-Reply-To: <1371563710.85433.YahooMailNeo@web163102.mail.bf1.yahoo.com> References: <1371563710.85433.YahooMailNeo@web163102.mail.bf1.yahoo.com> Message-ID: <761E2B5697F795489C8710BCC72141FF083F18@ex07.net.ucsf.edu> Rene, Thanks (again!) for putting the numbers up on this. It is one thing to say "not a good idea" but as has been said, if you can count it you can manage it, so it's the numbers that will convince people. Numbers help in the discussion by making the situation real. How about some numbers from everyone about how many blades they think is reasonable? Tim Morken UCSF Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, June 18, 2013 6:55 AM To: Teresa Moore; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Blade Rationing At 200 blocks/day x 5 days/week x 4 weeks/month = 4000 blocks/month which means that the 3 of you will have to use 1 blade every 80 blocks including trimming and sectioning which is ABSOLUTELY RIDICULOUS! The cost of blades, especially the better ones, are going up and you can save by using one blade to trim and another to?make the final section, but at the rate your manager wants the quality will be compromised. The "norm" (if there is a norm at all) is that a histotech will probably change blades every 5 to 10 blocks if the infiltration is good and there are no decals involved in the process. Lets assume that you can hold to 1 blade every 10 blocks, that will mean that during 1 month you will use 400 blades = 8 blades boxes. Find out how many you are actually using now and you will have an idea of your present blades usage. Additionally dull blades not only compromise the quality of the sections but also reduce sectioning productivity and what you may be saving in blades are going to increase in histotech time and total section production costs. Ren? J. From: Teresa Moore To: histonet@lists.utsouthwestern.edu Sent: Monday, June 17, 2013 5:10 PM Subject: [Histonet] Blade Rationing I work in a hospital, there are three of us on this particular shift and we cut approx. 200 blocks, give or take a few.? Our histo lab manager is telling us we should only be using one pack of blades (50 per pack) a month.? I'm wondering what other techs think of this especially lab managers and supervisors. tmoore9k@gmail.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Ruth.Riisnaes <@t> icr.ac.uk Tue Jun 18 10:18:41 2013 From: Ruth.Riisnaes <@t> icr.ac.uk (Ruth Riisnaes) Date: Tue Jun 18 10:19:04 2013 Subject: [Histonet] (no subject) In-Reply-To: <249931259.65611.1371563554576.JavaMail.mail@webmail13> References: <249931259.65611.1371563554576.JavaMail.mail@webmail13> Message-ID: Please remove me from the mailing list as well. Thanks. Ms Ruth Riisnaes Cancer Biomarkers Team MGN3, MUCRC The Institute of Cancer Research 15, Cotswold Road Sutton Surrey SM2 5NG Tel. 020 8643 8901 x 4778 Fax 020 8722 4084 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of pam@dlcjax.com Sent: 18 June 2013 14:53 To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Please remove me from the mailing list. Thanks Pam Mathews, CDC Dermatology and Laser Center Orange Park, Florida 32073 Office Manager 904-276-4500 Office 904-276-4160 Fax 904-945-6845 Cell _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The Institute of Cancer Research: Royal Cancer Hospital, a charitable Company Limited by Guarantee, Registered in England under Company No. 534147 with its Registered Office at 123 Old Brompton Road, London SW7 3RP. This e-mail message is confidential and for use by the addressee only. If the message is received by anyone other than the addressee, please return the message to the sender by replying to it and then delete the message from your computer and network. From campbellj <@t> muhlbauerlab.com Tue Jun 18 10:28:09 2013 From: campbellj <@t> muhlbauerlab.com (Jennifer Campbell) Date: Tue Jun 18 10:28:15 2013 Subject: [Histonet] New York Licensure In-Reply-To: <47E9B2C01DDDD94881EACD2DC44EBC880161D07C@D1PWPEXMBX05.mdanderson.edu> References: <47E9B2C01DDDD94881EACD2DC44EBC880161D07C@D1PWPEXMBX05.mdanderson.edu> Message-ID: <7A783F6B-5ACB-4AE2-8ECE-A47AC7ECA477@muhlbauerlab.com> They will need to take the HT and have the results sent to NYS for their license. At this time NYS does not recognize the HTL exam for licensure eligibility. Jen Campbell Sent from my iPhone On Jun 18, 2013, at 10:46 AM, "Mayer,Toysha N" wrote: > Good morning, > > This question is for those in the New York state area: What is needed for an HTL to become licensed to work in New York state. I have two students who are moving to the area after graduation (August), and will be eligible to sit for the ASCP HTL. The problem is the license requirements are listing HT as the qualifying certification. One student has contacted a recruiter and the state licensure agency and still is not sure what to do. > The state society said that since there are no HTL programs, a HT exam would have to be passed. Huh??? > The students are moving from Texas to New York state, and will hold a BS HTL, eligibility date for BOC of Aug 15. > Any help would be appreciated. > > > > Toysha N. Mayer, MBA, HT (ASCP) > Instructor, Education Coordinator > Program in Histotechnology > School of Health Professions > MD Anderson Cancer Center > (713) 563-3481 > tnmayer@mdanderson.org > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From benjamin <@t> histologistics.com Tue Jun 18 10:42:50 2013 From: benjamin <@t> histologistics.com (Benjamin) Date: Tue Jun 18 10:43:04 2013 Subject: [Histonet] Blade Rationing In-Reply-To: References: Message-ID: 200 blocks per day / 3 techs = 66 blocks per day per tech 66 blocks X 20 workdays a month = 1320 blocks a month 1320 blocks / 50 pack of blades = 26 blocks per blade Resonable or not? You decide. Sent from my iPhone On Jun 17, 2013, at 5:10 PM, Teresa Moore wrote: > I work in a hospital, there are three of us on this particular shift and we > cut approx. 200 blocks, give or take a few. Our histo lab manager is > telling us we should only be using one pack of blades (50 per pack) a > month. I'm wondering what other techs think of this especially lab > managers and supervisors. > > tmoore9k@gmail.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From contact <@t> excaliburpathology.com Tue Jun 18 10:47:39 2013 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Tue Jun 18 10:47:45 2013 Subject: [Histonet] Blade Rationing In-Reply-To: <51C06C9A.5080205@pigsqq.org> References: <1371563710.85433.YahooMailNeo@web163102.mail.bf1.yahoo.com> <51C06C9A.5080205@pigsqq.org> Message-ID: <1371570459.14035.YahooMailNeo@web5701.biz.mail.ne1.yahoo.com> Love this! Where is the like button! ? Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 8901 S. Santa Fe, Suite G Oklahoma City, OK 73139 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ________________________________ From: ""E. Wayne Johnson ???"" To: Rene J Buesa Cc: "histonet@lists.utsouthwestern.edu" ; Teresa Moore Sent: Tuesday, June 18, 2013 9:20 AM Subject: Re: Re: [Histonet] Blade Rationing A stingy person is called an Iron Rooster (tie gong ji) in Chinese. An iron rooster won't turn loose of even one feather. The scratches that appear on slides cut with blades that should have been changed --we call them iron rooster tracks.? Sort of a pun since many people think Chinese characters look like chicken tracks. We don't worry about what the blades cost.? Our clients demand good answers, and we send pictures of the lesions with our reports, so we need good results. On 3:59, Rene J Buesa wrote: > At 200 blocks/day x 5 days/week x 4 weeks/month = 4000 blocks/month which means that the 3 of you will have to use 1 blade every 80 blocks including trimming and sectioning which is ABSOLUTELY RIDICULOUS! > The cost of blades, especially the better ones, are going up and you can save by using one blade to trim and another to make the final section, but at the rate your manager wants the quality will be compromised. > The "norm" (if there is a norm at all) is that a histotech will probably change blades every 5 to 10 blocks if the infiltration is good and there are no decals involved in the process. > Lets assume that you can hold to 1 blade every 10 blocks, that will mean that during 1 month you will use 400 blades = blades boxes. > Find out how many you are actually using now and you will have an idea of your present blades usage. > Additionally dull blades not only compromise the quality of the sections but also reduce sectioning productivity and what you may be saving in blades are going to increase in histotech time and total section production costs. > Ren? J. > > From: Teresa Moore > To: histonet@lists.utsouthwestern.edu > Sent: Monday, June 17, 2013 5:10 PM > Subject: [Histonet] Blade Rationing > > > I work in a hospital, there are three of us on this particular shift and we > cut approx. 200 blocks, give or take a few.? Our histo lab manager is > telling us we should only be using one pack of blades (50 per pack) a > month.? I'm wondering what other techs think of this especially lab > managers and supervisors. > > tmoore9k@gmail.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Tue Jun 18 10:50:50 2013 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Tue Jun 18 10:50:55 2013 Subject: [Histonet] Blade Rationing In-Reply-To: <1371570459.14035.YahooMailNeo@web5701.biz.mail.ne1.yahoo.com> References: , <1371563710.85433.YahooMailNeo@web163102.mail.bf1.yahoo.com>, <51C06C9A.5080205@pigsqq.org>, <1371570459.14035.YahooMailNeo@web5701.biz.mail.ne1.yahoo.com> Message-ID: Ikxpa2UiIA0KDQoKCgpKb2VsbGUgV2VhdmVyIE1BT00sIEhUTCAoQVNDUCkgUUlIQw0KIA0KPiBE 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10:54:08 2013 Subject: FW: [Histonet] Blade Rationing In-Reply-To: References: Message-ID: -----Original Message----- From: Thomas Jasper Sent: Tuesday, June 18, 2013 8:52 AM To: 'Teresa Moore' Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Blade Rationing Teresa, I concur with the all the responses. It seems your lab manager is not grasping the technical reality you must work under. If you are being asked to save money on blades why not try some different brands or negotiate some better pricing? That is something the lab manager can work on. Also, I would think you are doing your best optimize the use of each blade. You should be able to get 3 good cutting areas per blade before they're spent. Another consideration is having some blades for facing in only. I'm guessing the manager is being pressured to cut cost. I would look in other areas and at other items. Blades are of too critical importance to mess around with much. Good luck, Tom Jasper Thomas Jasper HT (ASCP) BAS AP Supervisor Deaconess Hospital Evansville, IN -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Teresa Moore Sent: Monday, June 17, 2013 4:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Blade Rationing I work in a hospital, there are three of us on this particular shift and we cut approx. 200 blocks, give or take a few. Our histo lab manager is telling us we should only be using one pack of blades (50 per pack) a month. I'm wondering what other techs think of this especially lab managers and supervisors. tmoore9k@gmail.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Deaconess Health System. If you have received this email in error please notify the originator of the message. From lblazek <@t> digestivespecialists.com Tue Jun 18 10:53:18 2013 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Tue Jun 18 10:57:04 2013 Subject: [Histonet] Blade Rationing In-Reply-To: References: Message-ID: <5A2BD13465E061429D6455C8D6B40E391656A9D552@IBMB7Exchange.digestivespecialists.com> I don't think it's a matter of being reasonable or not, though I think 26 blocks is a bit high. The major issue is taking away the discretion of the tech to make a decision as to the quality of the slide they are producing. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Benjamin Sent: Tuesday, June 18, 2013 11:43 AM To: Teresa Moore Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Blade Rationing 200 blocks per day / 3 techs = 66 blocks per day per tech 66 blocks X 20 workdays a month = 1320 blocks a month 1320 blocks / 50 pack of blades = 26 blocks per blade Resonable or not? You decide. Sent from my iPhone On Jun 17, 2013, at 5:10 PM, Teresa Moore wrote: > I work in a hospital, there are three of us on this particular shift > and we cut approx. 200 blocks, give or take a few. Our histo lab > manager is telling us we should only be using one pack of blades (50 > per pack) a month. I'm wondering what other techs think of this > especially lab managers and supervisors. > > tmoore9k@gmail.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Tue Jun 18 11:00:03 2013 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Tue Jun 18 11:00:17 2013 Subject: [Histonet] Blade Rationing In-Reply-To: References: Message-ID: <761E2B5697F795489C8710BCC72141FF083FB5@ex07.net.ucsf.edu> "26 blocks per blade" That's good. At the price we pay for blades that would be less than $11.00 per day per tech (150 blocks/day average), OR LESS THAN 20 MIN OF PAY PER TECH!! If they were limiting their blades, how many minutes would they spend trying to get good sections, or how expensive would mistakes be if something was missed due to poor sections? However, I think some labs do try to save money this way. Our pathologists often comment how much better our output is thaN the consult slides we get -especially after we recut outside blocks and compare to the original slides. Tim Morken -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Benjamin Sent: Tuesday, June 18, 2013 8:43 AM To: Teresa Moore Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Blade Rationing 200 blocks per day / 3 techs = 66 blocks per day per tech 66 blocks X 20 workdays a month = 1320 blocks a month 1320 blocks / 50 pack of blades = 26 blocks per blade Resonable or not? You decide. Sent from my iPhone On Jun 17, 2013, at 5:10 PM, Teresa Moore wrote: > I work in a hospital, there are three of us on this particular shift > and we cut approx. 200 blocks, give or take a few. Our histo lab > manager is telling us we should only be using one pack of blades (50 > per pack) a month. I'm wondering what other techs think of this > especially lab managers and supervisors. > > tmoore9k@gmail.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From talulahgosh <@t> gmail.com Tue Jun 18 11:20:39 2013 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Tue Jun 18 11:20:45 2013 Subject: [Histonet] (no subject) In-Reply-To: References: <249931259.65611.1371563554576.JavaMail.mail@webmail13> Message-ID: No. "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." -Chuck Palahniuk, "Haunted" On Tue, Jun 18, 2013 at 11:18 AM, Ruth Riisnaes wrote: > Please remove me from the mailing list as well. > > Thanks. > > Ms Ruth Riisnaes > Cancer Biomarkers Team > MGN3, MUCRC > The Institute of Cancer Research > 15, Cotswold Road > Sutton > Surrey SM2 5NG > > Tel. 020 8643 8901 x 4778 > Fax 020 8722 4084 > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of pam@dlcjax.com > Sent: 18 June 2013 14:53 > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] (no subject) > > > Please remove me from the mailing list. > > > > Thanks > > > > Pam Mathews, CDC > Dermatology and Laser Center > Orange Park, Florida 32073 > Office Manager > 904-276-4500 Office > 904-276-4160 Fax > 904-945-6845 Cell > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > The Institute of Cancer Research: Royal Cancer Hospital, a charitable > Company Limited by Guarantee, Registered in England under Company No. > 534147 with its Registered Office at 123 Old Brompton Road, London SW7 3RP. > > This e-mail message is confidential and for use by the addressee only. If > the message is received by anyone other than the addressee, please return > the message to the sender by replying to it and then delete the message > from your computer and network. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From cpyse <@t> x-celllab.com Tue Jun 18 11:36:50 2013 From: cpyse <@t> x-celllab.com (Cindy Pyse) Date: Tue Jun 18 11:36:54 2013 Subject: [Histonet] Blade Rationing In-Reply-To: References: Message-ID: <003e01ce6c42$087093c0$1951bb40$@x-celllab.com> There is no reason to cut quality of the slides by rationing blades. You have just made your techs job harder, which will effect TAT and now will probably get numerous recuts not to mention phone calls from the pathologist reading the slides. I tell my techs use what you need. I purchase them the accu-edge blades so for me cost is not an issue. But this is coming from a tech turned manager so my stand is a little different. Cindy Cindy Pyse CLT, HT(ASCP) Laboratory Manager X-Cell Laboratories of WNY 20 Northpointe Parkway Ste 100 Amherst, NY 14228 716-250-9235 Ext. 232 cpyse@x-celllab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Teresa Moore Sent: Monday, June 17, 2013 5:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Blade Rationing I work in a hospital, there are three of us on this particular shift and we cut approx. 200 blocks, give or take a few. Our histo lab manager is telling us we should only be using one pack of blades (50 per pack) a month. I'm wondering what other techs think of this especially lab managers and supervisors. tmoore9k@gmail.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gagnone <@t> KGH.KARI.NET Tue Jun 18 11:44:12 2013 From: gagnone <@t> KGH.KARI.NET (Gagnon, Eric) Date: Tue Jun 18 11:44:20 2013 Subject: [Histonet] Blade Rationing / Blade Conservation Message-ID: <5F06C3AD0B27264CA20CFA986C87882E6FFEA5A6@EXCHANGEPV1.KGH.ON.CA> Desperately trying to salvage something positive out of this justifiably acrimonious thread...may I suggest the following blade conservation strategy, that though perhaps well-known, hasn't come up in this discussion yet. By using one blade as a trimming blade, the 'edge' on the next blade will be conserved for actual sectioning. Similarly, when cutting levels, one-half of a blade can be used for rough trimming, then the same blade pushed across into the cutting zone for the actual sectioning. Also, if during trimming a hard/calcified/stapled section is found, perform microtomy on that block last, after trying to minimize the negative effects on cutting. Perhaps by conserving blades in these and other ways, some cost-savings can be found for the penny-wise manager! Hope this helps, Eric Gagnon MLT Histology Laboratory Kingston General Hospital Kingston, Ontario, Canada >I work in a hospital, there are three of us on this particular shift and we cut approx. 200 blocks, give or take a few. Our histo lab manager is telling us we should only be using one pack of blades (50 per pack) a month. I'm wondering what other techs think of this especially lab managers and supervisors. tmoore9k@gmail.com From algranth <@t> email.arizona.edu Tue Jun 18 11:45:31 2013 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Tue Jun 18 11:45:35 2013 Subject: [Histonet] Blade Rationing In-Reply-To: <761E2B5697F795489C8710BCC72141FF083FB5@ex07.net.ucsf.edu> References: <761E2B5697F795489C8710BCC72141FF083FB5@ex07.net.ucsf.edu> Message-ID: <83FBFBB6-0848-4EDC-B09C-34EF582BE977@email.arizona.edu> Just wondering if your manager will be seeing any of these responses? Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 From TJohnson <@t> gnf.org Tue Jun 18 12:13:26 2013 From: TJohnson <@t> gnf.org (Teri Johnson) Date: Tue Jun 18 12:13:38 2013 Subject: [Histonet] Re: Blade Rationing Message-ID: <9F3CFEE76E51B64991C7485270890B4049783365@EX4.lj.gnf.org> >I work in a hospital, there are three of us on this particular shift and we cut approx. 200 blocks, give or take a few. Our histo lab manager is telling us we should only be using one pack of blades (50 per pack) a month. I'm wondering what other techs think of this especially lab managers and supervisors. tmoore9k@gmail.com > Just wondering if your manager will be seeing any of these responses? Andrea Grantham, HT (ASCP) Senior Research Specialist Just in case.... Have your lab manager demonstrate how they expect this to be done... using specimens from his/her family members. Teri Johnson Manager, Histology GNF - San Diego, CA 858-332-4752 From CThornton <@t> dahlchase.com Tue Jun 18 12:43:10 2013 From: CThornton <@t> dahlchase.com (Clare Thornton) Date: Tue Jun 18 12:43:14 2013 Subject: [Histonet] p40 antibody Message-ID: I have a 6ml bottle (predilute) of Biocare's polyclonal p40 antibody that expired 3/13. We can't use it anymore, but is there anyone out there who can? I'll be happy to send it to you. It seems like such a waste to throw out that amount of antibody. Clare J. Thornton, HTL(ASCP),QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com From litepath2000 <@t> yahoo.com Tue Jun 18 12:51:28 2013 From: litepath2000 <@t> yahoo.com (NYSHisto) Date: Tue Jun 18 12:51:32 2013 Subject: Subject: [Histonet] New York Licensure Message-ID: <1371577888.70410.YahooMailNeo@web141204.mail.bf1.yahoo.com> Hi Toysha Yes, this is correct.? It is unfortunate but this is the way the law has been written, much to our disappointment.I would be more than happy to speak to you directly about this and will give you a call asap. Luis ? ------- Luis Chiriboga Ph.D. President, New York State Histotechnological Society NYSHS Website: www.nyhisto.org NYSHS Message Board: http://tech.groups.yahoo.com/group/NYSHS1972/ This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email. ------------------------------ Message: 2 Date: Tue, 18 Jun 2013 14:46:53 +0000 From: "Mayer,Toysha N" Subject: [Histonet] New York Licensure To: "'histonet@lists.utsouthwestern.edu'" ??? Message-ID: ??? <47E9B2C01DDDD94881EACD2DC44EBC880161D07C@D1PWPEXMBX05.mdanderson.edu> Content-Type: text/plain; charset="us-ascii" Good morning, This question is for those in the New York state area:? What is needed for an HTL to become licensed to work in New York state.? I have two students who are moving to the area after graduation (August), and will be eligible to sit for the ASCP HTL.? The problem is the license requirements are listing HT as the qualifying certification.? One student has contacted a recruiter and the state licensure agency and still is not sure what to do.? The state society said that since there are no HTL programs, a HT exam would have to be passed.? Huh??? The students are moving from Texas to New York state, and will hold a BS HTL, eligibility date for BOC of Aug 15. Any help would be appreciated. Toysha N. Mayer, MBA, HT (ASCP) Instructor, Education Coordinator Program in Histotechnology School of Health Professions MD Anderson Cancer Center (713) 563-3481 tnmayer@mdanderson.org From tmoore9k <@t> gmail.com Tue Jun 18 13:22:57 2013 From: tmoore9k <@t> gmail.com (Teresa Moore) Date: Tue Jun 18 13:23:01 2013 Subject: [Histonet] Blade Rationing Follow-up Message-ID: I really appreciate everyone's constructive comments regarding my post on blade rationing. Lots of you said there are many other ways to cut costs in the lab. I would like to hear some of your suggestions so I can take them back to my manager. I'd like to give her some legitimate alternatives to her proposal. Would like to contribute to solving the problem of cutting costs. Thanks again Teresa Moore, HT From JMaslanka <@t> stpetes.org Tue Jun 18 13:27:10 2013 From: JMaslanka <@t> stpetes.org (JMaslanka@stpetes.org) Date: Tue Jun 18 13:28:15 2013 Subject: [Histonet] IHC Tech Competency Checklist Message-ID: Anyone willing to share IHC Technician Competency checklist? Thanks in advance Joe Maslanka BS, CT,HT (ASCP) Anatomical Pathology Technical Supervisor St Peter's Hospital,MT 59601 (P)(406) 447-2406 (F)(406)444-2126 Give thanks for ALL things..... "Kindness is the language the blind can see & the deaf can hear- Mark Twain This electronic mail message contains information which is confidential. If you are not the intended recipient, please be aware that any disclosure, photocopying, distribution or use of the contents of the received information is prohibited. If you have received this e-mail in error, please reply to the sender immediately and permanently delete this message and all copies of it. Thank you. From Sherrian.McAnn <@t> va.gov Tue Jun 18 13:32:12 2013 From: Sherrian.McAnn <@t> va.gov (McAnn, Sherrian) Date: Tue Jun 18 13:32:48 2013 Subject: [Histonet] Cutting paraffin sections on a cryostat operated at roomtemperature? Nope. Message-ID: <61E2B58CECEF384094A363989D47C09009E9BFBF@VHAV17MSGA2.v17.med.va.gov> Thank You! If you have ever had to break one down and clean and put it back together you would know it is difficult (at best) to even turn the handle. These are precisely machined and don't operate well ...if at all when warm. I suppose though that you could cut your sections cold , however seems like it would be awkward. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Harrison, Sandra C. Sent: Monday, June 17, 2013 8:30 AM To: Johnson, Kevin; histonet@lists.utsouthwestern.edu Subject: [Histonet] Cutting paraffin sections on a cryostat operated at roomtemperature? Nope. Ever tried turning the handle of the cryostat, when it's at room temperature? Cryostats are tooled & manufactured to operate at a low temperature. Since metal contracts at the low temperature, you'll find that you can't operate the microtome at the higher temperature. The handle will barely move. Sandy Harrison, HTL (ASCP) Histology Supervisor Minneapolis VAHCS 612-467-2449 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Johnson, Kevin Sent: Friday, June 14, 2013 3:09 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Cutting paraffin sections...on a cryostat? Hi, all. A bit of an odd question: a colleague knows of someone wanting to cut paraffin sections who has a cryostat, but no microtome. Since a cryostat's basically a microtome in a freezer chamber, I thought that it may be awkward, but theoretically doable once it was brought to room temp and dried out thoroughly. However, I wondered if lubricants formulated for the cold might become too thin for use at room temp, possibly causing damage to moving parts. Any thoughts? Kevin Johnson University of Miami Diabetes Research Institute _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Allison.Scott <@t> harrishealth.org Tue Jun 18 13:55:33 2013 From: Allison.Scott <@t> harrishealth.org (Scott, Allison D) Date: Tue Jun 18 13:55:38 2013 Subject: [Histonet] Free standing outpatient surgery center Message-ID: Hello to all in histo land. In January we will have an outpatient surgery center that will have a frozen section lab located there. We are now in the stages of figuring out how we will get specimens from there to our main histology lab. Is there anyone out there that has the same sort of set up and how are you managing it . We already have the equipment selected that we need its all of the other pieces that are making it interesting. Allison Scott HT(ASCP) Supervisor, Histology Lab LBJ Hospital Harris Health System Office: 713-566-2148 Lab: 713-566-5287 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. From natalia_zinchenko <@t> hotmail.com Tue Jun 18 14:03:54 2013 From: natalia_zinchenko <@t> hotmail.com (Natalia Zinchenko) Date: Tue Jun 18 14:04:02 2013 Subject: [Histonet] RE: Histonet Digest, Vol 115, Issue 17 In-Reply-To: References: Message-ID: Hello Histonet! I'm a long time reader first time poster. Does anyone have experience processing guinea pig tissues? I have been processing kidney and heart but it is consistently coming out mushy in the middle. The mouse tissue comes out fine even when processed on the same run. I had the tissue grossed in thinner (2.5mm) thinking that perhaps it was too thick but it didn't seem to help. Also, it has been fixed in 10%NBF for several days. I was just wondering if anyone else had similar problems with guinea pig? I appreciate in advance any advice or tips. Here is the protocol: Formalin 1hr 70% etOH 1hr 95% 1hr 100% 30min 100% 1hr 100% 1hr 100%1hr Clearify 1hr Clearify 1hr Clearify 1hr Paraffin 1hr paraffin 1hr All under pressure and heat only on the paraffin. Thanks Heather I work with guinea pig soft and decalcified bone specimens for several years. Here is my protocol: Fixation in 10% NBF from24 to 48 hours. Switch to 70% ethanol or do the infiltration 70% etOh 1 hr 80% 1 hr 95% 1 hr 95% 1 hr 100% 1 hr 100% 1 hr 100% 1 hr Xylene 1 hr Xylene 1 hr Xylene 1 hr Wax58 1 hr Wax58 1 hr Wax58 1 hr Wax58 1 hr > From: histonet-request@lists.utsouthwestern.edu > Subject: Histonet Digest, Vol 115, Issue 17 > To: histonet@lists.utsouthwestern.edu > Date: Mon, 17 Jun 2013 10:00:39 -0700 > > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. re 2050 microtome (Steven Weston) > 2. Cutting paraffin sections on a cryostat operated at room > temperature? Nope. (Harrison, Sandra C.) > 3. RE: Paraffin processing native sheep ACL (Jack Ratliff) > 4. Picro Sirius Red Stain (John Shelley) > 5. Processing Guinea Pig (Heather Marlatt) > 6. Re: Picro Sirius Red Stain (Grantham, Andrea L - (algranth)) > 7. Cryostat Repair Service in San Diego (dusko trajkovic) > 8. Re: Picro Sirius Red Stain (Laura Avogaro) > 9. ACIS CALIBRATION SLIDE SET (Breal, Kari) > 10. Re: Processing Guinea Pig (Grantham, Andrea L - (algranth)) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Mon, 17 Jun 2013 11:10:17 +0000 > From: Steven Weston > Subject: [Histonet] re 2050 microtome > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > <7B808A2E6BDDBD4EA4FA6395FF7BED2B5E7E2402@MBXSBYN2.utas.ad.internal> > Content-Type: text/plain; CHARSET=US-ASCII > > This could be as simple as moving the specimen holder forward. It may be racked right into the microtome and therefore show as stopped. > > Try moving the holder forward and see if the stopped light goes off. > > Regards > > > steve weston > lab manager > Breathe-Well CRE > UTAS-SOM > > > > > > Message: 9 > Date: Sat, 15 Jun 2013 00:07:51 -0300 > From: "C.D.G." > Subject: [Histonet] manual setup for Reichert-Jung 2050 needed > To: histonet@lists.utsouthwestern.edu > Cc: histonet@lists.utsouthwestern.edu > Message-ID: <201306150007510574.0040B781@smtp.montevideo.com.uy> > Content-Type: text/plain; charset="ISO-8859-1" > > Hi all: > i received a Reichert-Jung microtome 2050 model. I need instructions for its operation. > The electronic panel displays "stop" illuminated and I dont know how to continue, as other > buttons seems not to operate. Any help of people who has worked or know to operate this > motorized microtome will be appreciated. > My kind regards, > Carlos Defeo > Histotechnologist > > > > > > > ------------------------------ > > Message: 2 > Date: Mon, 17 Jun 2013 08:30:06 -0500 > From: "Harrison, Sandra C." > Subject: [Histonet] Cutting paraffin sections on a cryostat operated > at room temperature? Nope. > To: "Johnson, Kevin" , > > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > Ever tried turning the handle of the cryostat, when it's at room > temperature? > Cryostats are tooled & manufactured to operate at a low temperature. > Since metal contracts at the low temperature, you'll find that you can't > operate the microtome at the higher temperature. The handle will barely > move. > > Sandy Harrison, HTL (ASCP) > Histology Supervisor > Minneapolis VAHCS > 612-467-2449 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Johnson, > Kevin > Sent: Friday, June 14, 2013 3:09 PM > To: 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] Cutting paraffin sections...on a cryostat? > > Hi, all. A bit of an odd question: a colleague knows of someone wanting > to cut paraffin sections who has a cryostat, but no microtome. Since a > cryostat's basically a microtome in a freezer chamber, I thought that it > may be awkward, but theoretically doable once it was brought to room > temp and dried out thoroughly. However, I wondered if lubricants > formulated for the cold might become too thin for use at room temp, > possibly causing damage to moving parts. Any thoughts? > > Kevin Johnson > University of Miami > Diabetes Research Institute > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 3 > Date: Mon, 17 Jun 2013 09:54:53 -0400 > From: Jack Ratliff > Subject: RE: [Histonet] Paraffin processing native sheep ACL > To: Andrew Prior , Histonet > , "lizronan@umich.edu" > > Cc: Jack Ratliff > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > You might also consider using methyl salicylate instead of xylenes. Thanks to the help of Bob Skinner, I have achieved very nice results with native tendon. > Generally speaking these MS steps will take a little longer, but you can monitor the progress very easily by watching for complete transparency of the tendon. You can then even develop a somewhat standardized protocol if you plan to process this type of tissue in the future. You even have a lot more flexibility with MS than xylenes as prolonged use in xylenes can make the tissue more hardened and brittle. > Lastly, it is not generally recommended to put MS on the tissue processor, so I process to the final 100% EtOH, perform the MS exchanges by hand, transfer the tissues into a manual wax step to get rid of as much MS as possible and then finish with three (3) automated wax steps on the tissue processor. > For my wax infiltration I use a 50:50 blend of TissuePrep from Fisher Scientific and EM400 from Leica. I then embed in 100% EM400. > Best Regards, > Jack > > > Jack L RatliffOwner/Histologist, Ratliff Histology Consultants, LLCChairman, Hard Tissue Committee - National Society for Histotechnology > > > > > From: a.prior@tissueregenix.com > > To: histonet@lists.utsouthwestern.edu > > Date: Fri, 14 Jun 2013 07:45:47 +0000 > > Subject: Re: [Histonet] Paraffin processing native sheep ACL > > CC: > > > > Hi Liz, > > > > > > > > I inherited the following protocol for ACL samples. It works quite well, but times probably could be reduced - the optimization is on my to-do list. > > > > 70% Alcohol - 1 hour > > > > 90% alcohol - 1 hour > > > > 100% Alcohol -2 hours > > > > 100% alcohol - 3 hours > > > > 100% alcohol - 4 hours > > > > Xylene - 1.5 hours > > > > Xylene - 1.5 hours > > > > Xylene - 3 hours > > > > Wax - 3 hours > > > > Wax - 3 hours > > > > Wax - 4 hours > > > > I cut the sections at 8um so they hold together better. Takes a while for all the wrinkles to disappear when floating out on water-bath so be patient > > > > Hope this helps. > > > > > > > > Andrew > > > > > > Andrew Prior > > Histologist > > Tissue Regenix Group > > E-mail: a.prior@tissueregenix.com > > Website: www.tissueregenix.com > > > > > > > > ------------------------------ > > > > Message: 3 > > > > Date: Wed, 12 Jun 2013 16:59:52 -0400 > > > > From: Elizabeth Ronan > > > > > Subject: [Histonet] Paraffin processing native sheep ACL > > > > To: histonet@lists.utsouthwestern.edu > > > > Message-ID: <51B8E148.3020102@umich.edu> > > > > Content-Type: text/plain; charset=ISO-8859-1; format=flowed > > > > > > > > Hello, > > > > > > > > I need to paraffin process native sheep anterior cruciate ligament (ACL) that has been fixed in 10% neutral buffered formalin for 7 days. I was wondering if anyone with more expertise on this subject could guide me with the best lengths of times in the alcohols, xylenes, and paraffin to fix ACL. I tried a 12 hour program but the sections crumbled in the middle and it appeared that the paraffin had not fully perfused the ligament. > > > > > > > > I have access to the following program, and can alter the lengths of the steps for as long as desired: > > > > > > > > Program: > > > > 70% > > > > 80% > > > > 95% > > > > 95% > > > > 100% > > > > 100% > > > > Xylene > > > > Xylene > > > > Paraffin > > > > Paraffin > > > > Paraffin > > > > > > > > Any advice is much appreciated. > > > > Thanks for your time, > > > > Liz > > > > > > > > The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited. If you received this in error, please contact the sender and delete the material from any computer. Although we routinely screen for viruses, addressees should check this e-mail and any attachment for viruses. We make no warranty as to absence of viruses in this e-mail or any attachments. > > > > Registered Office: The Biocentre, Innovation Way, Heslington, York, YO10 5NY > > Registered No. 05969271 > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Message: 4 > Date: Mon, 17 Jun 2013 14:30:03 +0000 > From: John Shelley > Subject: [Histonet] Picro Sirius Red Stain > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > Hi All, > > Hope all are having a great Monday!!!! > > I am trying to do a Picro Sirius Red stain and have looked at many protocols that seem to not use a particular reagent that is in a manufacturers kit. I was hoping if someone could enlighten me as to why this reagent would be used and if someone is using it at what strength is the PhosoMolybdic Acid concentration. > > Your help will be greatly appreciated!!!! > > Kind Regards! > > John J Shelley > Research Specialist, Histology Core Facility > > > > ------------------------------ > > Message: 5 > Date: Mon, 17 Jun 2013 07:33:21 -0700 > From: Heather Marlatt > Subject: [Histonet] Processing Guinea Pig > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > > Content-Type: text/plain; charset=ISO-8859-1 > > Hello Histonet! I'm a long time reader first time poster. Does anyone have > experience processing guinea pig tissues? I have been processing kidney and > heart but it is consistently coming out mushy in the middle. The mouse > tissue comes out fine even when processed on the same run. I had the tissue > grossed in thinner (2.5mm) thinking that perhaps it was too thick but it > didn't seem to help. Also, it has been fixed in 10%NBF for several days. > > I was just wondering if anyone else had similar problems with guinea pig? > > I appreciate in advance any advice or tips. > > Here is the protocol: > > Formalin 1hr > 70% etOH 1hr > 95% 1hr > 100% 30min > 100% 1hr > 100% 1hr > 100%1hr > Clearify 1hr > Clearify 1hr > Clearify 1hr > Paraffin 1hr > paraffin 1hr > > > All under pressure and heat only on the paraffin. > > Thanks > Heather > > > ------------------------------ > > Message: 6 > Date: Mon, 17 Jun 2013 15:28:30 +0000 > From: "Grantham, Andrea L - (algranth)" > Subject: Re: [Histonet] Picro Sirius Red Stain > To: John Shelley > Cc: "histonet@lists.utsouthwestern.edu" > > Message-ID: <02DCE952-FD4D-432E-BB62-84AD17A507D6@email.arizona.edu> > Content-Type: text/plain; charset="us-ascii" > > I do a Picro Sirius Red stain for collagen (got the protocol from Gayle Callis) and don't use Phosphomolybdic acid. > > My protocol is simple: > one hour in PSR > two rinses in acidified water > rinse > you can counterstain but my clients don't want a counterstain so I dehydrate, clear and coverslip. > Boom, I'm done. > > The PSR stain is just Sirius Red F3B 0.5 gms and 500 ml of Sat. Picric Acid. Do not use a dye that is not CI 35780. > The acidified water is 5ml Glacial Acetic Acid to 1L of DH2O > > Under polarized light the PSR stain is gorgeous! I love looking at the orange, yellow, pinks and green colors. Good thing because I do this stain all the time. > > Andrea Grantham, HT (ASCP) > Senior Research Specialist > University of Arizona > Cellular and Molecular Medicine > Histology Service Laboratory > P.O.Box 245044 > Tucson, AZ 85724 > > algranth@email.arizona.edu > Tel: 520.626.4415 Fax: 520.626.2097 > > > > > > > > ------------------------------ > > Message: 7 > Date: Mon, 17 Jun 2013 08:35:43 -0700 (PDT) > From: dusko trajkovic > Subject: [Histonet] Cryostat Repair Service in San Diego > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > <1371483343.29781.YahooMailNeo@web181703.mail.ne1.yahoo.com> > Content-Type: text/plain; charset=us-ascii > > > > Good Morning, > Can anyone recomend a good, reliable cryostat repair service in the San diego area? > thanks > Dusko > > ------------------------------ > > Message: 8 > Date: Mon, 17 Jun 2013 17:35:44 +0200 (CEST) > From: "Laura Avogaro" > Subject: Re: [Histonet] Picro Sirius Red Stain > To: "John Shelley" > Cc: histonet@lists.utsouthwestern.edu > Message-ID: > <3388.192.168.178.78.1371483344.squirrel@www.science.unitn.it> > Content-Type: text/plain;charset=iso-8859-1 > > Dear John, > I usually perform Picrosirius Red stain in cardiac tisse sections. > I use phosphomolibic acid treatment in order to eliminate the cytoplasmic > staining improving collagen/myoplasm contrast. > > Have a look at this paper: > Picrosirius red staining of cardiac muscle following phosphomolybdic acid > treatment > Dolber PC, Spach MS > Stain Technol. 1987 Jan;62(1):23-6. > > Good luck! > > Laura > > > > Hi All, > > > > Hope all are having a great Monday!!!! > > > > I am trying to do a Picro Sirius Red stain and have looked at many > > protocols that seem to not use a particular reagent that is in a > > manufacturers kit. I was hoping if someone could enlighten me as to why > > this reagent would be used and if someone is using it at what strength is > > the PhosoMolybdic Acid concentration. > > > > Your help will be greatly appreciated!!!! > > > > Kind Regards! > > > > John J Shelley > > Research Specialist, Histology Core Facility > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > Laura Avogaro > University of Trento > Via delle Regole, 101 38123 Mattarello (TN) ? Italy > Tel: +39 0461 283425 > > > > > ------------------------------ > > Message: 9 > Date: Mon, 17 Jun 2013 10:40:32 -0500 > From: "Breal, Kari" > Subject: [Histonet] ACIS CALIBRATION SLIDE SET > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > > > Content-Type: text/plain; charset="us-ascii" > > Does anyone have an ACIS Calibration set they are not using and are willing to part with? One of my slides broke and I can't find a replacement anywhere. > > Thanks, > Kari Breal > > > Kari Breal, HT (ASCP) > Histology Manager > Alexian Brothers Health System > ABMC-847-437-5500 ext. 5155 > SAMC-847-843-2000 ext. 6818 > kari.breal@alexian.net > > > > > > CONFIDENTIALITY NOTICE: > This email message and any accompanying data or files is confidential and may contain privileged information intended only for the named recipient(s). If you are not the intended recipient(s), you are hereby notified that the dissemination, distribution, and or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at the email address above, delete this email from your computer, and destroy any copies in any form immediately. Receipt by anyone other than the named recipient(s) is not a waiver of any attorney-client, work product, or other applicable privilege. > > ------------------------------ > > Message: 10 > Date: Mon, 17 Jun 2013 15:50:09 +0000 > From: "Grantham, Andrea L - (algranth)" > Subject: Re: [Histonet] Processing Guinea Pig > Cc: "histonet@lists.utsouthwestern.edu" > > Message-ID: > Content-Type: text/plain; charset="us-ascii" > > Heather, > Looking at your protocol for processing the kidney and heart tissue I can't figure out why it is mushy especially if you are putting it in the cassettes so thin and it has had a chance to fix well before processing. In fact, looking at your protocol I might think that your tissue might be dried out and need to sit in cold icy water before sectioning. > > At any rate, I would rinse well in running water after fixing and skip the formalin on the processor. > > I'd start in 70% alcohol, maybe two changes and move on up to 80%, 2- 95%'s and 3-100%'s. > I'm not familiar with the clearing agent that you use. I use Clear Rite 3 and never have a problem - 2 changes of Clear Rite 3 and 4 paraffins. > > I don't know how many cassettes you are processing but make sure there is adequate room for the reagents on the processor to move around the cassettes and that you have a good ratio of the reagents to the tissue and that the reagents are fresh. If you can increase the agitation in some of the dehydration steps it might help. > > > Andrea Grantham, HT (ASCP) > Senior Research Specialist > University of Arizona > Cellular and Molecular Medicine > Histology Service Laboratory > P.O.Box 245044 > Tucson, AZ 85724 > > algranth@email.arizona.edu > Tel: 520.626.4415 Fax: 520.626.2097 > > > > > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 115, Issue 17 > ***************************************** From c.tague <@t> Pathologyarts.com Tue Jun 18 14:35:59 2013 From: c.tague <@t> Pathologyarts.com (Curt) Date: Tue Jun 18 14:36:05 2013 Subject: [Histonet] Blade Rationing Follow-up In-Reply-To: References: Message-ID: <9C8F910F72893643B3C3793C3D67132B013A3A99@PATHOLOGYSERVER.pathologyarts.local> You could suggest that she switch to part-time... :) That's probably not a good idea. How about recycling your alcohol and Xylene/Xylene substitute? Are you already doing that? I'm not sure what others are looking at as far as cost to process per block but we recycle all 100% and Xylene, change the processors 2x/wk and have a cost of about $0.11 per block. I think we're the biggest hit is the paraffin, about 1 cs/wk. formalin is cheap, Xylene is all recycled and alcohol is all recycled but a couple gallons of the 100% which are obviously run virgin reagents. I've found significant savings. I'm curious if others are managing their costs at this level too, if so, what are your numbers looking like? Curt Tague, CEO, Pathology Arts -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Teresa Moore Sent: Tuesday, June 18, 2013 11:23 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Blade Rationing Follow-up I really appreciate everyone's constructive comments regarding my post on blade rationing. Lots of you said there are many other ways to cut costs in the lab. I would like to hear some of your suggestions so I can take them back to my manager. I'd like to give her some legitimate alternatives to her proposal. Would like to contribute to solving the problem of cutting costs. Thanks again Teresa Moore, HT _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Jun 18 14:37:39 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jun 18 14:37:42 2013 Subject: [Histonet] Blade Rationing Follow-up In-Reply-To: References: Message-ID: <1371584259.44164.YahooMailNeo@web163105.mail.bf1.yahoo.com> I understand your point about telling your manager where to cut costs, but that is YOUR MANAGER'S job for which s/he is for sure better paid than you are. Let s/he figure that out! Just warn your manager about the loss of quality with a measure like the one you have been asked to comply with. You are better off if you discuss this issue with the lab director. Ren? J. From: Teresa Moore To: histonet@lists.utsouthwestern.edu Sent: Tuesday, June 18, 2013 2:22 PM Subject: [Histonet] Blade Rationing Follow-up I really appreciate everyone's constructive comments regarding my post on blade rationing.? Lots of you said there are many other ways to cut costs in the lab.? I would like to hear some of your suggestions so I can take them back to my manager.? I'd like to give her some legitimate alternatives to her proposal. Would like to contribute to solving the problem of cutting costs. Thanks again Teresa Moore, HT _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From c.tague <@t> Pathologyarts.com Tue Jun 18 14:41:08 2013 From: c.tague <@t> Pathologyarts.com (Curt) Date: Tue Jun 18 14:41:13 2013 Subject: [Histonet] Blade Rationing Follow-up In-Reply-To: <9C8F910F72893643B3C3793C3D67132B013A3A99@PATHOLOGYSERVER.pathologyarts.local> References: <9C8F910F72893643B3C3793C3D67132B013A3A99@PATHOLOGYSERVER.pathologyarts.local> Message-ID: <9C8F910F72893643B3C3793C3D67132B013A3AF4@PATHOLOGYSERVER.pathologyarts.local> Had a thought change in the middle of a sentence, forgive the grammatical error, we're.... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Curt Sent: Tuesday, June 18, 2013 12:36 PM To: Teresa Moore; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Blade Rationing Follow-up You could suggest that she switch to part-time... :) That's probably not a good idea. How about recycling your alcohol and Xylene/Xylene substitute? Are you already doing that? I'm not sure what others are looking at as far as cost to process per block but we recycle all 100% and Xylene, change the processors 2x/wk and have a cost of about $0.11 per block. I think we're the biggest hit is the paraffin, about 1 cs/wk. formalin is cheap, Xylene is all recycled and alcohol is all recycled but a couple gallons of the 100% which are obviously run virgin reagents. I've found significant savings. I'm curious if others are managing their costs at this level too, if so, what are your numbers looking like? Curt Tague, CEO, Pathology Arts -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Teresa Moore Sent: Tuesday, June 18, 2013 11:23 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Blade Rationing Follow-up I really appreciate everyone's constructive comments regarding my post on blade rationing. Lots of you said there are many other ways to cut costs in the lab. I would like to hear some of your suggestions so I can take them back to my manager. I'd like to give her some legitimate alternatives to her proposal. Would like to contribute to solving the problem of cutting costs. Thanks again Teresa Moore, HT _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From c.tague <@t> Pathologyarts.com Tue Jun 18 14:50:16 2013 From: c.tague <@t> Pathologyarts.com (Curt) Date: Tue Jun 18 14:50:21 2013 Subject: [Histonet] Blade Rationing Follow-up In-Reply-To: <1371584259.44164.YahooMailNeo@web163105.mail.bf1.yahoo.com> References: <1371584259.44164.YahooMailNeo@web163105.mail.bf1.yahoo.com> Message-ID: <9C8F910F72893643B3C3793C3D67132B013A3B4A@PATHOLOGYSERVER.pathologyarts.local> I'd send an email to both of them, show's them that you're being proactive and taking the initiative to look out for patient care AND the best interests of the lab/hosp. Include the lab director to get credit for your high level of care, so the manager doesn't take all the credit. This is how you move up the food chain, prove you're more valuable then what they're currently using you for. Curt -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, June 18, 2013 12:38 PM To: Teresa Moore; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Blade Rationing Follow-up I understand your point about telling your manager where to cut costs, but that is YOUR MANAGER'S job for which s/he is for sure better paid than you are. Let s/he figure that out! Just warn your manager about the loss of quality with a measure like the one you have been asked to comply with. You are better off if you discuss this issue with the lab director. Ren? J. From: Teresa Moore To: histonet@lists.utsouthwestern.edu Sent: Tuesday, June 18, 2013 2:22 PM Subject: [Histonet] Blade Rationing Follow-up I really appreciate everyone's constructive comments regarding my post on blade rationing.? Lots of you said there are many other ways to cut costs in the lab.? I would like to hear some of your suggestions so I can take them back to my manager.? I'd like to give her some legitimate alternatives to her proposal. Would like to contribute to solving the problem of cutting costs. Thanks again Teresa Moore, HT _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Matthew.Reardon <@t> petermac.org Tue Jun 18 19:59:04 2013 From: Matthew.Reardon <@t> petermac.org (Reardon Matthew) Date: Tue Jun 18 20:00:03 2013 Subject: [Histonet] NX70 Cryostat Message-ID: Hello, I was wondering if anyone has any experience with the NX70 Cryostat made by ThermoFisher? Thanks, Matt This email (including any attachments or links) may contain confidential and/or legally privileged information and is intended only to be read or used by the addressee. If you are not the intended addressee, any use, distribution, disclosure or copying of this email is strictly prohibited. Confidentiality and legal privilege attached to this email (including any attachments) are not waived or lost by reason of its mistaken delivery to you. If you have received this email in error, please delete it and notify us immediately by telephone or email. Peter MacCallum Cancer Centre provides no guarantee that this transmission is free of virus or that it has not been intercepted or altered and will not be liable for any delay in its receipt. From rmhickey88 <@t> gmail.com Tue Jun 18 21:50:17 2013 From: rmhickey88 <@t> gmail.com (Ryan Hickey) Date: Tue Jun 18 21:50:22 2013 Subject: [Histonet] HTL Currently Seeking Employment Message-ID: Hello Histonet, and thank you for the opportunity to introduce myself: My name is Ryan Hickey, and I will soon be graduating from a NAACLS-accredited 1-year program in Histotechnology from The University of Texas M.D. Anderson Cancer Center School of Health Professions. I am in the final stage of my clinical rotations both in immunohistochemistry and biorepository research, after accruing experience in immunohistochemistry, special stains, microtomy, cryotomy, grossing, and laboratory operations. Throughout the duration of the program, I feel that I have received a strong foundation of knowledge and clinical practice in histotechnology. Prior to my clinical education in histotechnology, I spent significant time in a molecular profiling and diagnostics laboratory practicing immunohistochemical and immunofluorescence assays. My work comprised protocol validation and optimization in regards to the characterization of biomarkers related to squamous cell carcinomas. In August, I will be eligible for ASCP Histotechnologist Certification (HTL); my application for the certification exam has been submitted. If selected, I am able to begin work immediately. Please feel free to contact me if you or any of your colleagues or associates are seeking a well-qualified, dedicated histotechnologist. Thank you for taking the time to review my information and consider my request; as always, I look forward to contributing constructively to the Histonet in the near future. Kind regards, Ryan M. Hickey, BS From rmhickey88 <@t> gmail.com Tue Jun 18 22:08:12 2013 From: rmhickey88 <@t> gmail.com (Ryan Hickey) Date: Tue Jun 18 22:08:16 2013 Subject: [Histonet] Re: HTL Currently Seeking Employment In-Reply-To: References: Message-ID: You may contact me at rmhickey88@gmail.com On Tue, Jun 18, 2013 at 9:50 PM, Ryan Hickey wrote: > Hello Histonet, and thank you for the opportunity to introduce myself: > > > My name is Ryan Hickey, and I will soon be graduating from a > NAACLS-accredited 1-year program in Histotechnology from The University of > Texas M.D. Anderson Cancer Center School of Health Professions. I am in the > final stage of my clinical rotations both in immunohistochemistry and > biorepository research, after accruing experience in immunohistochemistry, > special stains, microtomy, cryotomy, grossing, and laboratory operations. > Throughout the duration of the program, I feel that I have received a > strong foundation of knowledge and clinical practice in histotechnology. > > Prior to my clinical education in histotechnology, I spent significant > time in a molecular profiling and diagnostics laboratory practicing > immunohistochemical and immunofluorescence assays. My work comprised > protocol validation and optimization in regards to the characterization of > biomarkers related to squamous cell carcinomas. > > In August, I will be eligible for ASCP Histotechnologist Certification > (HTL); my application for the certification exam has been submitted. If > selected, I am able to begin work immediately. > > Please feel free to contact me if you or any of your colleagues or > associates are seeking a well-qualified, dedicated histotechnologist. Thank > you for taking the time to review my information and consider my request; > as always, I look forward to contributing constructively to the Histonet in > the near future. > > > Kind regards, > > Ryan M. Hickey, BS > > From tony.henwood <@t> health.nsw.gov.au Tue Jun 18 23:11:49 2013 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Tue Jun 18 23:12:04 2013 Subject: [Histonet] Blade Rationing Follow-up In-Reply-To: References: Message-ID: <6D6BD1DE8A5571489398B392A38A71579D28242C@xmdb04.nch.kids> Good Answer!!! ;) Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Teresa Moore Sent: Wednesday, 19 June 2013 4:23 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Blade Rationing Follow-up I really appreciate everyone's constructive comments regarding my post on blade rationing. Lots of you said there are many other ways to cut costs in the lab. I would like to hear some of your suggestions so I can take them back to my manager. I'd like to give her some legitimate alternatives to her proposal. Would like to contribute to solving the problem of cutting costs. Thanks again Teresa Moore, HT _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From Jonathan.Cremer <@t> med.kuleuven.be Wed Jun 19 01:47:31 2013 From: Jonathan.Cremer <@t> med.kuleuven.be (Jonathan Cremer) Date: Wed Jun 19 01:47:50 2013 Subject: [Histonet] RE: RE:Blade Rationing In-Reply-To: References: Message-ID: Do you know that you can buy graduated glass pipettes which can be washed and reused many, many, many, many times, made for that exact purpose? You can also autclave them, or bake them at 180 ?C for sterility. At least you wouldn't have the issue of components starting to leach from the plastic after reusing them several times... I'd rather consider resharpening disposable blades instead of washing dispoble plastic pipettes. --- Jonathan Cremer Laboratory Technician ________________________________________ Van: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] namens Smith, Allen [asmith@mail.barry.edu] Verzonden: dinsdag 18 juni 2013 16:53 To: histonet@lists.utsouthwestern.edu Onderwerp: [Histonet] RE:Blade Rationing I run an academic lab on a very tight budget. A paper towel used to dry washed hands is used again. Outdated dye solutions are adsorbed onto a small pile of old paper towels to save on waste disposal costs. (A quarter-pound of solid waste costs less to dispose of than 2 liters of aqueous liquid waste.) Disposable pipettes are washed and reused until the numbers wear off. I make up Vector's ImmPact SG 1.7 ml a time, store it in the fridge, and use it all week. I don't save on microtome blades. Dull blades leave holes in 4 micron sections. Sections cut with a dull blade have the annoying habit of exploding on the water bath. Dull blades tease out collagen fibers and drape them over the cells I'm trying to study. When a blade is dull it goes into the sharps box. -Allen A. Smith, Ph.D. Barry University School of Podiatric Medicine Miami Shores, FL _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Wed Jun 19 07:06:47 2013 From: joelleweaver <@t> hotmail.com (=?utf-8?B?am9lbGxld2VhdmVyQGhvdG1haWwuY29t?=) Date: Wed Jun 19 07:06:53 2013 Subject: =?utf-8?B?UmU6IFtIaXN0b25ldF0gSUhDIFRlY2ggQ29tcGV0ZW5jeSBDaGVja2xpc3Q=?= Message-ID: Give me more specifics, platforms? Manual? Theory? Or training checklists? Maybe can help, finishing mine now Sent from my Verizon Wireless 4G LTE Smartphone ----- Reply message ----- From: JMaslanka@stpetes.org To: Subject: [Histonet] IHC Tech Competency Checklist Date: Tue, Jun 18, 2013 2:27 pm Anyone willing to share IHC Technician Competency checklist? Thanks in advance Joe Maslanka BS, CT,HT (ASCP) Anatomical Pathology Technical Supervisor St Peter's Hospital,MT 59601 (P)(406) 447-2406 (F)(406)444-2126 Give thanks for ALL things..... "Kindness is the language the blind can see & the deaf can hear- Mark Twain This electronic mail message contains information which is confidential. If you are not the intended recipient, please be aware that any disclosure, photocopying, distribution or use of the contents of the received information is prohibited. If you have received this e-mail in error, please reply to the sender immediately and permanently delete this message and all copies of it. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From portera <@t> msu.edu Wed Jun 19 08:31:50 2013 From: portera <@t> msu.edu (Amy Porter) Date: Wed Jun 19 08:31:33 2013 Subject: [Histonet] IHC on Gerbil Tissue Message-ID: <001601ce6cf1$5b07f790$1117e6b0$@edu> Is anyone out there performing IHC with Mouse primaries on gerbil tissue??? If so what are you using for secondaries? Amy S. Porter, HT(ASCP) QIHC Michigan State University Investigative HistoPathology Laboratory William S. Spielman, Ph.D. - Director Department of Physiology / Human Pathology Biomedical Physical Sciences Building 567 Wilson Road - Room 2133 East Lansing, MI 48824-3320 Phone: 517-884-5026 Fax: 517-432-1368 portera@msu.edu www.humanpathology.msu.edu From Elizabeth.Cameron <@t> jax.org Wed Jun 19 09:37:28 2013 From: Elizabeth.Cameron <@t> jax.org (Elizabeth Cameron) Date: Wed Jun 19 09:37:34 2013 Subject: [Histonet] Cutting cells on PTFE membrane? Message-ID: Does anyone have any experience with cutting cells on PTFE (polytetrafluoroethylene) membrane? Are there any issues cutting it with standard FFPE methods? Thanks in advance, Liz The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as possible. From sdysart <@t> mirnarx.com Wed Jun 19 09:51:35 2013 From: sdysart <@t> mirnarx.com (Sarah Dysart) Date: Wed Jun 19 09:51:46 2013 Subject: [Histonet] FAB fragments Message-ID: I am going to try using a FAB fragment to block some mouse on mouse IHC staining...the protocol I am looking at says to dilute in PBS. My question is can I dilute it with my normal dilution buffer (DAKO, background reducing dilution buffer...which I'm sure is mostly PBS...), or should I using PBS? Thanks Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 From ahorvath <@t> cogipath.com Wed Jun 19 10:19:08 2013 From: ahorvath <@t> cogipath.com (Andrew Horvath) Date: Wed Jun 19 10:19:48 2013 Subject: [Histonet] Blade Rationing Follow-up In-Reply-To: <6D6BD1DE8A5571489398B392A38A71579D28242C@xmdb04.nch.kids> References: <6D6BD1DE8A5571489398B392A38A71579D28242C@xmdb04.nch.kids> Message-ID: One suggestion is to make sure purchasing is being done through a group purchasing organization (GPO) (i.e., MedAssets, Amerinet, or Provista). A second is to review contracts that are currently in place to identify if changes could be made to lower cost vendors. I would anticipate the lab manager has done these things but these are places to start. You could volunteer to assist in these activities to increase your value to the organization. Andrew Horvath, MBA, MA Interim Operations Manager 303-770-4848 (O) 303-770-6641 (fax) Colorado GI Pathology 7346 S. Alton Way Suite 10E Centennial, CO 80112 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony Henwood (SCHN) Sent: Tuesday, June 18, 2013 10:12 PM To: 'Teresa Moore'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Blade Rationing Follow-up Good Answer!!! ;) Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Teresa Moore Sent: Wednesday, 19 June 2013 4:23 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Blade Rationing Follow-up I really appreciate everyone's constructive comments regarding my post on blade rationing. Lots of you said there are many other ways to cut costs in the lab. I would like to hear some of your suggestions so I can take them back to my manager. I'd like to give her some legitimate alternatives to her proposal. Would like to contribute to solving the problem of cutting costs. Thanks again Teresa Moore, HT _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ************************************************************************ ********* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ************************************************************************ ********* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From algranth <@t> email.arizona.edu Wed Jun 19 10:35:56 2013 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Wed Jun 19 10:36:07 2013 Subject: [Histonet] Cutting cells on PTFE membrane? In-Reply-To: References: Message-ID: <404A7CEF-AB49-4308-A633-475188CDB550@email.arizona.edu> I cut cells grown on membranes pretty often and just process them along with the other tissue. I usually put the membranes into a histoscreen cassette - don't wrap it or put anything on top of the membrane. On embedding I usually cut the membrane into 2-3 sections and embed standing up to see the cells and how they attach to the membrane. Works well - no problems. Andi Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 From saby_joseph_a <@t> yahoo.com Wed Jun 19 11:39:08 2013 From: saby_joseph_a <@t> yahoo.com (Joseph Saby) Date: Wed Jun 19 11:39:16 2013 Subject: [Histonet] Joseph Saby Message-ID: <1371659948.5374.YahooMailNeo@web163001.mail.bf1.yahoo.com> dshos http://bran-denschools.org/hui/jdul/jifh/pbiaq.htm Joseph Saby mhphq From BMolinari <@t> texasheart.org Wed Jun 19 12:32:26 2013 From: BMolinari <@t> texasheart.org (Molinari, Betsy) Date: Wed Jun 19 12:32:31 2013 Subject: [Histonet] Karnovsky's Message-ID: Hi, I received a heart that was fixed in 2% Karnovsky?s. I was told it was initially kept under refrigeration but then stored at RT. I do not know how long the heart has been stored at RT. By the look of the tissue I would say quite a while. Is this tissue compromised in any way? Should I remove it from the Karnovsky?s and store in in something else? This heart is to undergo paraffin processing. Thanks for any input. Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave MC1-283 Houston,TX 77030 832-355-6524 (lab) 832-355-6812 (fax) Betsy Molinari Senior Histology Research Technician 832-355-6524 | BMolinari@texasheart.org | www.texasheart.org 6770 Bertner Ave., MC 1-283, Houston, TX 77030 [Texas Heart Institute][THI News] [THI on Facebook] [THI on Flicker] [THI on Google] [THI on Pinterest] [THI on Twitter] [THI on You Tube] Confidentiality Notice: This message may be confidential and/or privileged. If you are not the intended recipient you may not review, disseminate or copy this e-mail, its contents and/or any attachments. Please immediately notify the sender If you have received this e-mail in error and delete it from your computer system. From lguernsey <@t> ucsd.edu Wed Jun 19 13:01:10 2013 From: lguernsey <@t> ucsd.edu (Lucie Guernsey) Date: Wed Jun 19 13:02:00 2013 Subject: [Histonet] Mixing Paraffin Brands Message-ID: Hi, Does anyone know if there's a reason why one shouldn't mix different brands of paraffin? We normally use Fisherbrand's Paraplast Plus (melting point 56 C). We inherited about 8 bags of McCormick Paraplast X-tra (melting point 52 C). Will the different melting points be a problem? If we were to use the McCormick paraffin, the only place it may mix with the Fisherbrand paraffin is in the blocks themselves (as we refill the embedder). But I don't want to compromise the quality of our blocks just to not waste the free paraffin. Or, another option could be that we use the McCormick in the processor and the Fisherbrand in the embedder. Could that cause issues in the blocks as the tissue would be infiltrated with one brand and embedded in another? Maybe I'm over-thinking this...... Many thanks! Lucie Lucie Guernsey UCSD Dept. of Pathology lguernsey@ucsd.edu From rjbuesa <@t> yahoo.com Wed Jun 19 13:20:41 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jun 19 13:20:47 2013 Subject: [Histonet] Karnovsky's In-Reply-To: References: Message-ID: <1371666041.21504.YahooMailNeo@web163106.mail.bf1.yahoo.com> Are you referring to a whole heart? If that is the case I think the fixation of the tissue will be very poor because Karnovsky's penetrate very slowly. I do not think it will be worth the effort and materials to process this tissue. Ren? J. From: "Molinari, Betsy" To: "Histonet@lists.utsouthwestern.edu" Sent: Wednesday, June 19, 2013 1:32 PM Subject: [Histonet] Karnovsky's Hi, I received a heart that was fixed in 2% Karnovsky?s. I was told it was initially kept under refrigeration but then stored at RT. I do not know how long? the heart has been stored at RT. By the look of the tissue I would say quite a while. Is this tissue compromised in any way? Should I remove it from the Karnovsky?s and store in in something else? This heart is to undergo paraffin processing. Thanks for any input. Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave MC1-283 Houston,TX 77030 832-355-6524 (lab) 832-355-6812 (fax) Betsy Molinari Senior Histology Research Technician 832-355-6524 | BMolinari@texasheart.org | www.texasheart.org 6770 Bertner Ave., MC 1-283, Houston, TX 77030 [Texas Heart Institute][THI News] [THI on Facebook] ? [THI on Flicker] ? [THI on Google] ? [THI on Pinterest] ? [THI on Twitter] ? [THI on You Tube] Confidentiality Notice: This message may be confidential and/or privileged. If you are not the intended recipient you may not review, disseminate or copy this e-mail, its contents and/or any attachments. Please immediately notify the sender If you have received this e-mail in error and delete it from your computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mtighe <@t> trudeauinstitute.org Wed Jun 19 13:22:44 2013 From: mtighe <@t> trudeauinstitute.org (Mike Tighe) Date: Wed Jun 19 13:22:54 2013 Subject: [Histonet] Tissue Tek VIP 3000 Message-ID: <135069b13312463f90a9987ee100f133@BY2PR07MB074.namprd07.prod.outlook.com> In an internet search I came across a used TissueTek VIP 3000 that is in "excellent condition". This looks to be an older model and I am wondering if anyone has any experience with this instrument? Are there any issues with parts replacement? comes with one year warranty and they are asking around $8000.00. Thanks! Mike From rjbuesa <@t> yahoo.com Wed Jun 19 13:24:27 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jun 19 13:24:32 2013 Subject: [Histonet] Mixing Paraffin Brands In-Reply-To: References: Message-ID: <1371666267.45338.YahooMailNeo@web163104.mail.bf1.yahoo.com> Each paraffin has some additives to improve either its penetration rate or density to section. Mixing different melting points (MP) paraffin will result in another paraffin with an intermediate MP and the sectioning will be different. Preparing the block with the mixture will probably cause so troubles while sectioning. Why don't you just use them separate? There is no good reason to mix them and the two paraffins you mention are of good quality. Ren? J. From: Lucie Guernsey To: histonet@lists.utsouthwestern.edu Sent: Wednesday, June 19, 2013 2:01 PM Subject: [Histonet] Mixing Paraffin Brands Hi, Does anyone know if there's a reason why one shouldn't mix different brands of paraffin? We normally use Fisherbrand's Paraplast Plus (melting point 56 C). We inherited about 8 bags of McCormick Paraplast X-tra (melting point 52 C). Will the different melting points be a problem? If we were to use the McCormick paraffin, the only place it may mix with the Fisherbrand paraffin is in the blocks themselves (as we refill the embedder). But I don't want to compromise the quality of our blocks just to not waste the free paraffin. Or, another option could be that we use the McCormick in the processor and the Fisherbrand in the embedder. Could that cause issues in the blocks as the tissue would be infiltrated with one brand and embedded in another? Maybe I'm over-thinking this...... Many thanks! Lucie Lucie Guernsey UCSD Dept. of Pathology lguernsey@ucsd.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Jun 19 13:27:17 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jun 19 13:27:21 2013 Subject: [Histonet] Tissue Tek VIP 3000 In-Reply-To: <135069b13312463f90a9987ee100f133@BY2PR07MB074.namprd07.prod.outlook.com> References: <135069b13312463f90a9987ee100f133@BY2PR07MB074.namprd07.prod.outlook.com> Message-ID: <1371666437.41983.YahooMailNeo@web163105.mail.bf1.yahoo.com> According with the model and brand the price is very good. Why don't you contact Sakura to find about parts for it in existence or even a maintenance contract with them? Sakura VIP are top of the line in tissue processing. Ren? J. From: Mike Tighe To: "histonet@lists.utsouthwestern.edu (histonet@lists.utsouthwestern.edu)" Sent: Wednesday, June 19, 2013 2:22 PM Subject: [Histonet] Tissue Tek VIP 3000 In an internet search I came across a used TissueTek VIP 3000 that is in "excellent condition". This looks to be an older model and I am wondering if anyone has any experience with this instrument? Are there any issues with parts replacement? comes with one year warranty and they are asking around $8000.00. Thanks! Mike _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DSiena <@t> statlab.com Wed Jun 19 13:29:45 2013 From: DSiena <@t> statlab.com (Debra Siena) Date: Wed Jun 19 13:29:49 2013 Subject: [Histonet] Mixing Paraffin Brands In-Reply-To: References: Message-ID: Hi Lucie, Paraplast Plus has DMSO and Paraplast Xtra doesn't. It may be best to infiltrate with the Plus which is softer and then embed with the Paraplast Xtra which is a bit harder. I don't usually recommend mixing them especially if you only have a few bags. Thanks Debbie Siena 800.442.3573 ext. 229 | www.statlab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lucie Guernsey Sent: Wednesday, June 19, 2013 1:01 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Mixing Paraffin Brands Hi, Does anyone know if there's a reason why one shouldn't mix different brands of paraffin? We normally use Fisherbrand's Paraplast Plus (melting point 56 C). We inherited about 8 bags of McCormick Paraplast X-tra (melting point 52 C). Will the different melting points be a problem? If we were to use the McCormick paraffin, the only place it may mix with the Fisherbrand paraffin is in the blocks themselves (as we refill the embedder). But I don't want to compromise the quality of our blocks just to not waste the free paraffin. Or, another option could be that we use the McCormick in the processor and the Fisherbrand in the embedder. Could that cause issues in the blocks as the tissue would be infiltrated with one brand and embedded in another? Maybe I'm over-thinking this...... Many thanks! Lucie Lucie Guernsey UCSD Dept. of Pathology lguernsey@ucsd.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LouroP <@t> Princeton.Huntingdon.com Wed Jun 19 13:32:33 2013 From: LouroP <@t> Princeton.Huntingdon.com (Louro, Pedro) Date: Wed Jun 19 13:32:40 2013 Subject: [Histonet] Mixing Paraffin Brands References: <1371666267.45338.YahooMailNeo@web163104.mail.bf1.yahoo.com> Message-ID: Just out of curiosity, what paraffin would people out there recommend using for animal bone joints and turbinates? We are currently using parapalst plus and was thinking of changing to a "harder paraffin" Any thoughts??? Pedro L. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, June 19, 2013 2:24 PM To: Lucie Guernsey; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Mixing Paraffin Brands Each paraffin has some additives to improve either its penetration rate or density to section. Mixing different melting points (MP) paraffin will result in another paraffin with an intermediate MP and the sectioning will be different. Preparing the block with the mixture will probably cause so troubles while sectioning. Why don't you just use them separate? There is no good reason to mix them and the two paraffins you mention are of good quality. Ren? J. From: Lucie Guernsey To: histonet@lists.utsouthwestern.edu Sent: Wednesday, June 19, 2013 2:01 PM Subject: [Histonet] Mixing Paraffin Brands Hi, Does anyone know if there's a reason why one shouldn't mix different brands of paraffin? We normally use Fisherbrand's Paraplast Plus (melting point 56 C). We inherited about 8 bags of McCormick Paraplast X-tra (melting point 52 C). Will the different melting points be a problem? If we were to use the McCormick paraffin, the only place it may mix with the Fisherbrand paraffin is in the blocks themselves (as we refill the embedder). But I don't want to compromise the quality of our blocks just to not waste the free paraffin. Or, another option could be that we use the McCormick in the processor and the Fisherbrand in the embedder. Could that cause issues in the blocks as the tissue would be infiltrated with one brand and embedded in another? Maybe I'm over-thinking this...... Many thanks! Lucie Lucie Guernsey UCSD Dept. of Pathology lguernsey@ucsd.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************************************************************************************** Our Values: Excellence, Drive, Ownership, Challenge, Teamwork, Respect ******************************************************************************************** LEGAL NOTICE This message is confidential and contains information which may be legally privileged. It is intended for the stated addressee(s) only. Access to this email by anyone else is unauthorised. If you are not the intended addressee, any disclosure or copying of the contents of this email or any action taken (or not taken) in reliance on it is unauthorised and is unlawful. If you are not the addressee, please inform the sender immediately and destroy the original message. The data in this email will have been screened for the presence of computer viruses known by the Company at the time the email was produced. The Company cannot guarantee, however, that the email or any attachments are virus free. Delivery of the email will be at the Client's risk. Registered in England No. 1815730 VAT Reg. No. GB425507072 Registered Office: Woolley Road, Alconbury, Huntingdon, Cambridgeshire PE28 4HS ********************************************************************** From MDiCarlo <@t> KaleidaHealth.Org Wed Jun 19 13:39:16 2013 From: MDiCarlo <@t> KaleidaHealth.Org (DiCarlo, Margaret) Date: Wed Jun 19 13:39:23 2013 Subject: [Histonet] Mixing Paraffin Brands In-Reply-To: References: <1371666267.45338.YahooMailNeo@web163104.mail.bf1.yahoo.com> Message-ID: <731BE09CDB19AA43AA8682199D42D31B05494D0A@ADCEXCHANGE01.KaleidaHealth.org> I use Tissue Prep 2 for human bones and have previously used it for mice bone joints. It works very well for both. Peggy DiCarlo -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Louro, Pedro Sent: Wednesday, June 19, 2013 14:33 To: Rene J Buesa; Lucie Guernsey; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Mixing Paraffin Brands Just out of curiosity, what paraffin would people out there recommend using for animal bone joints and turbinates? We are currently using parapalst plus and was thinking of changing to a "harder paraffin" Any thoughts??? Pedro L. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, June 19, 2013 2:24 PM To: Lucie Guernsey; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Mixing Paraffin Brands Each paraffin has some additives to improve either its penetration rate or density to section. Mixing different melting points (MP) paraffin will result in another paraffin with an intermediate MP and the sectioning will be different. Preparing the block with the mixture will probably cause so troubles while sectioning. Why don't you just use them separate? There is no good reason to mix them and the two paraffins you mention are of good quality. Ren? J. From: Lucie Guernsey To: histonet@lists.utsouthwestern.edu Sent: Wednesday, June 19, 2013 2:01 PM Subject: [Histonet] Mixing Paraffin Brands Hi, Does anyone know if there's a reason why one shouldn't mix different brands of paraffin? We normally use Fisherbrand's Paraplast Plus (melting point 56 C). We inherited about 8 bags of McCormick Paraplast X-tra (melting point 52 C). Will the different melting points be a problem? If we were to use the McCormick paraffin, the only place it may mix with the Fisherbrand paraffin is in the blocks themselves (as we refill the embedder). But I don't want to compromise the quality of our blocks just to not waste the free paraffin. Or, another option could be that we use the McCormick in the processor and the Fisherbrand in the embedder. Could that cause issues in the blocks as the tissue would be infiltrated with one brand and embedded in another? Maybe I'm over-thinking this...... Many thanks! Lucie Lucie Guernsey UCSD Dept. of Pathology lguernsey@ucsd.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************************************************************************************** Our Values: Excellence, Drive, Ownership, Challenge, Teamwork, Respect ******************************************************************************************** LEGAL NOTICE This message is confidential and contains information which may be legally privileged. It is intended for the stated addressee(s) only. Access to this email by anyone else is unauthorised. If you are not the intended addressee, any disclosure or copying of the contents of this email or any action taken (or not taken) in reliance on it is unauthorised and is unlawful. If you are not the addressee, please inform the sender immediately and destroy the original message. The data in this email will have been screened for the presence of computer viruses known by the Company at the time the email was produced. The Company cannot guarantee, however, that the email or any attachments are virus free. Delivery of the email will be at the Client's risk. Registered in England No. 1815730 VAT Reg. No. GB425507072 Registered Office: Woolley Road, Alconbury, Huntingdon, Cambridgeshire PE28 4HS ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The Keeping You Informed section of Kaleida Health`s website features a wealth of information, stories and pictures about our valued workforce and the tremendous momentum our organization is experiencing. Check us out at: www.kaleidahealth.org/kyi CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. 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From philip_manfre <@t> merck.com Wed Jun 19 13:40:12 2013 From: philip_manfre <@t> merck.com (Manfre, Philip) Date: Wed Jun 19 13:40:33 2013 Subject: [Histonet] Mixing Paraffin Brands In-Reply-To: References: Message-ID: <558A4571351D0C42BD923F403F4198C4AC554C9886@USCTMXP51014.merck.com> Lucie, Apparently I only responded to you directly. I am re-sending my response in case anyone else on the net is interested. Phil. Lucie, Personally, I would not mix them, as they have somewhat different properties, such as the melting point. I think you are asking for trouble, or inconsistent results at least, when compared to your normal product. If you have more than one processor and embedding center, set up one of each with the other paraffin and use it on low-priority tissue, if such exists. At least the infiltration and embedding matrix will be the same. Just my opinion. Phil Philip Manfre, B.A., HT (ASCP) Associate Principal Scientist Merck Research Laboratories WP45-251 PO Box 4 West Point, PA 19486 215-652-9750 215-993-0383 (fax) philip_manfre@merck.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lucie Guernsey Sent: Wednesday, June 19, 2013 2:01 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Mixing Paraffin Brands Hi, Does anyone know if there's a reason why one shouldn't mix different brands of paraffin? We normally use Fisherbrand's Paraplast Plus (melting point 56 C). We inherited about 8 bags of McCormick Paraplast X-tra (melting point 52 C). Will the different melting points be a problem? If we were to use the McCormick paraffin, the only place it may mix with the Fisherbrand paraffin is in the blocks themselves (as we refill the embedder). But I don't want to compromise the quality of our blocks just to not waste the free paraffin. Or, another option could be that we use the McCormick in the processor and the Fisherbrand in the embedder. Could that cause issues in the blocks as the tissue would be infiltrated with one brand and embedded in another? Maybe I'm over-thinking this...... Many thanks! Lucie Lucie Guernsey UCSD Dept. of Pathology lguernsey@ucsd.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From philip_manfre <@t> merck.com Wed Jun 19 13:42:05 2013 From: philip_manfre <@t> merck.com (Manfre, Philip) Date: Wed Jun 19 13:42:09 2013 Subject: [Histonet] Mixing Paraffin Brands In-Reply-To: References: <1371666267.45338.YahooMailNeo@web163104.mail.bf1.yahoo.com> Message-ID: <558A4571351D0C42BD923F403F4198C4AC554C988B@USCTMXP51014.merck.com> Hey Pedro, How are you? We sometimes use T-555, which is a little firmer. Phil. Philip Manfre, B.A., HT (ASCP) Associate Principal Scientist Merck Research Laboratories WP45-251 PO Box 4 West Point, PA 19486 215-652-9750 215-993-0383 (fax) philip_manfre@merck.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Louro, Pedro Sent: Wednesday, June 19, 2013 2:33 PM To: Rene J Buesa; Lucie Guernsey; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Mixing Paraffin Brands Just out of curiosity, what paraffin would people out there recommend using for animal bone joints and turbinates? We are currently using parapalst plus and was thinking of changing to a "harder paraffin" Any thoughts??? Pedro L. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, June 19, 2013 2:24 PM To: Lucie Guernsey; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Mixing Paraffin Brands Each paraffin has some additives to improve either its penetration rate or density to section. Mixing different melting points (MP) paraffin will result in another paraffin with an intermediate MP and the sectioning will be different. Preparing the block with the mixture will probably cause so troubles while sectioning. Why don't you just use them separate? There is no good reason to mix them and the two paraffins you mention are of good quality. Ren? J. From: Lucie Guernsey To: histonet@lists.utsouthwestern.edu Sent: Wednesday, June 19, 2013 2:01 PM Subject: [Histonet] Mixing Paraffin Brands Hi, Does anyone know if there's a reason why one shouldn't mix different brands of paraffin? We normally use Fisherbrand's Paraplast Plus (melting point 56 C). We inherited about 8 bags of McCormick Paraplast X-tra (melting point 52 C). Will the different melting points be a problem? If we were to use the McCormick paraffin, the only place it may mix with the Fisherbrand paraffin is in the blocks themselves (as we refill the embedder). But I don't want to compromise the quality of our blocks just to not waste the free paraffin. Or, another option could be that we use the McCormick in the processor and the Fisherbrand in the embedder. Could that cause issues in the blocks as the tissue would be infiltrated with one brand and embedded in another? Maybe I'm over-thinking this...... Many thanks! Lucie Lucie Guernsey UCSD Dept. of Pathology lguernsey@ucsd.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************************************************************************************** Our Values: Excellence, Drive, Ownership, Challenge, Teamwork, Respect ******************************************************************************************** LEGAL NOTICE This message is confidential and contains information which may be legally privileged. It is intended for the stated addressee(s) only. Access to this email by anyone else is unauthorised. If you are not the intended addressee, any disclosure or copying of the contents of this email or any action taken (or not taken) in reliance on it is unauthorised and is unlawful. If you are not the addressee, please inform the sender immediately and destroy the original message. The data in this email will have been screened for the presence of computer viruses known by the Company at the time the email was produced. The Company cannot guarantee, however, that the email or any attachments are virus free. Delivery of the email will be at the Client's risk. Registered in England No. 1815730 VAT Reg. No. GB425507072 Registered Office: Woolley Road, Alconbury, Huntingdon, Cambridgeshire PE28 4HS ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From JGarfield <@t> lifecell.com Wed Jun 19 14:17:22 2013 From: JGarfield <@t> lifecell.com (Garfield, Jacqueline) Date: Wed Jun 19 14:18:03 2013 Subject: [Histonet] Mixing Paraffin Brands In-Reply-To: References: <1371666267.45338.YahooMailNeo@web163104.mail.bf1.yahoo.com> Message-ID: <76C6970EFD29CA46A2F105DE3737CE0904081C5939@AMWPVEX03.kci.com> Pedro, I recommend Paraplast-Xtra. Regards, Jackie Jacqueline D. Garfield | Manager, Histology Main 908.947.1100 Fax 908.947.1085 Direct 908.947.1182 Email jgarfield@ lifecell.com www.lifcell.com LifeCell Corporation | One Millennium Way | Branchburg, NJ | 08876 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Louro, Pedro Sent: Wednesday, June 19, 2013 2:33 PM To: Rene J Buesa; Lucie Guernsey; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Mixing Paraffin Brands Just out of curiosity, what paraffin would people out there recommend using for animal bone joints and turbinates? We are currently using parapalst plus and was thinking of changing to a "harder paraffin" Any thoughts??? Pedro L. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, June 19, 2013 2:24 PM To: Lucie Guernsey; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Mixing Paraffin Brands Each paraffin has some additives to improve either its penetration rate or density to section. Mixing different melting points (MP) paraffin will result in another paraffin with an intermediate MP and the sectioning will be different. Preparing the block with the mixture will probably cause so troubles while sectioning. Why don't you just use them separate? There is no good reason to mix them and the two paraffins you mention are of good quality. Ren? J. From: Lucie Guernsey To: histonet@lists.utsouthwestern.edu Sent: Wednesday, June 19, 2013 2:01 PM Subject: [Histonet] Mixing Paraffin Brands Hi, Does anyone know if there's a reason why one shouldn't mix different brands of paraffin? We normally use Fisherbrand's Paraplast Plus (melting point 56 C). We inherited about 8 bags of McCormick Paraplast X-tra (melting point 52 C). Will the different melting points be a problem? If we were to use the McCormick paraffin, the only place it may mix with the Fisherbrand paraffin is in the blocks themselves (as we refill the embedder). But I don't want to compromise the quality of our blocks just to not waste the free paraffin. Or, another option could be that we use the McCormick in the processor and the Fisherbrand in the embedder. Could that cause issues in the blocks as the tissue would be infiltrated with one brand and embedded in another? Maybe I'm over-thinking this...... Many thanks! Lucie Lucie Guernsey UCSD Dept. of Pathology lguernsey@ucsd.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************************************************************************************** Our Values: Excellence, Drive, Ownership, Challenge, Teamwork, Respect ******************************************************************************************** LEGAL NOTICE This message is confidential and contains information which may be legally privileged. It is intended for the stated addressee(s) only. Access to this email by anyone else is unauthorised. If you are not the intended addressee, any disclosure or copying of the contents of this email or any action taken (or not taken) in reliance on it is unauthorised and is unlawful. If you are not the addressee, please inform the sender immediately and destroy the original message. The data in this email will have been screened for the presence of computer viruses known by the Company at the time the email was produced. The Company cannot guarantee, however, that the email or any attachments are virus free. Delivery of the email will be at the Client's risk. Registered in England No. 1815730 VAT Reg. No. GB425507072 Registered Office: Woolley Road, Alconbury, Huntingdon, Cambridgeshire PE28 4HS ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Thomas.Jasper <@t> deaconess.com Wed Jun 19 14:20:04 2013 From: Thomas.Jasper <@t> deaconess.com (Thomas Jasper) Date: Wed Jun 19 14:20:09 2013 Subject: [Histonet] Blade Rationing Follow-up In-Reply-To: References: Message-ID: You could start with cost comparison of blades (since this whole thing started as a blade issue). Reps are willing to let you demo some before committing and you might save money if everyone likes a less expensive blade. You can do the same for paraffin and slides as well as reagent alcohols and xylene. Again, these are things the manager should be getting after. Does your lab adhere to Lean principles? There may be some cost savings there if you can accomplish tasks more efficiently; get the work done in less time or cutting back on overtime. It's a bit difficult to say as much depends on the nature and scope of your service. All situations are unique - do you send a lot of work out? Could you take it on in-house? Or are you trying to do things in-house that could be sent out and have a cost positive effect? Lots of questions for the manager... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Teresa Moore Sent: Tuesday, June 18, 2013 1:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Blade Rationing Follow-up I really appreciate everyone's constructive comments regarding my post on blade rationing. Lots of you said there are many other ways to cut costs in the lab. I would like to hear some of your suggestions so I can take them back to my manager. I'd like to give her some legitimate alternatives to her proposal. Would like to contribute to solving the problem of cutting costs. Thanks again Teresa Moore, HT _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Deaconess Health System. If you have received this email in error please notify the originator of the message. From ratliffjack <@t> hotmail.com Wed Jun 19 14:51:59 2013 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Wed Jun 19 14:52:03 2013 Subject: [Histonet] Mixing Paraffin Brands In-Reply-To: <76C6970EFD29CA46A2F105DE3737CE0904081C5939@AMWPVEX03.kci.com> References: , <1371666267.45338.YahooMailNeo@web163104.mail.bf1.yahoo.com>, , <76C6970EFD29CA46A2F105DE3737CE0904081C5939@AMWPVEX03.kci.com> Message-ID: Pedro, I have been using a superb method from Bob Skinner for all my decalcified bone work. Basically, I infiltrate with 50% TissuePrep from Fisher Scientific and 50% EM400 from Leica. I then embed in straight EM400. I personally feel that I get good infiltration and I never have had a problem with my bone cutting. (knocking twice on wood....LOL) I might also mention that I decalcify with 5% formic acid and clear after dehydration using methyl salicylate instead of xylenes. Jack Jack L RatliffOwner/Histologist, Ratliff Histology Consultants, LLCChairman, Hard Tissue Committee - National Society for Histotechnology > From: JGarfield@lifecell.com > To: LouroP@Princeton.Huntingdon.com; rjbuesa@yahoo.com; lguernsey@ucsd.edu; histonet@lists.utsouthwestern.edu > Date: Wed, 19 Jun 2013 14:17:22 -0500 > Subject: RE: [Histonet] Mixing Paraffin Brands > CC: > > Pedro, > > I recommend Paraplast-Xtra. > > Regards, > Jackie > > Jacqueline D. Garfield | Manager, Histology > > Main 908.947.1100 Fax 908.947.1085 > Direct 908.947.1182 > Email jgarfield@ lifecell.com > www.lifcell.com > > LifeCell Corporation | One Millennium Way | Branchburg, NJ | 08876 > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Louro, Pedro > Sent: Wednesday, June 19, 2013 2:33 PM > To: Rene J Buesa; Lucie Guernsey; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Mixing Paraffin Brands > > Just out of curiosity, what paraffin would people out there recommend using for animal bone joints and turbinates? > We are currently using parapalst plus and was thinking of changing to a "harder paraffin" > > Any thoughts??? > > Pedro L. > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa > Sent: Wednesday, June 19, 2013 2:24 PM > To: Lucie Guernsey; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Mixing Paraffin Brands > > Each paraffin has some additives to improve either its penetration rate or density to section. > Mixing different melting points (MP) paraffin will result in another paraffin with an intermediate MP and the sectioning will be different. > Preparing the block with the mixture will probably cause so troubles while sectioning. > Why don't you just use them separate? There is no good reason to mix them and the two paraffins you mention are of good quality. > Ren? J. > > From: Lucie Guernsey > To: histonet@lists.utsouthwestern.edu > Sent: Wednesday, June 19, 2013 2:01 PM > Subject: [Histonet] Mixing Paraffin Brands > > > Hi, > > Does anyone know if there's a reason why one shouldn't mix different brands > of paraffin? We normally use Fisherbrand's Paraplast Plus (melting point 56 > C). We inherited about 8 bags of McCormick Paraplast X-tra (melting point > 52 C). Will the different melting points be a problem? > > If we were to use the McCormick paraffin, the only place it may mix with > the Fisherbrand paraffin is in the blocks themselves (as we refill the > embedder). But I don't want to compromise the quality of our blocks just to > not waste the free paraffin. > > Or, another option could be that we use the McCormick in the processor and > the Fisherbrand in the embedder. Could that cause issues in the blocks as > the tissue would be infiltrated with one brand and embedded in another? > > Maybe I'm over-thinking this...... > > Many thanks! > Lucie > > Lucie Guernsey > UCSD > Dept. of Pathology > lguernsey@ucsd.edu > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ******************************************************************************************** > Our Values: Excellence, Drive, Ownership, Challenge, Teamwork, Respect > ******************************************************************************************** > > LEGAL NOTICE > This message is confidential and contains information which may be legally privileged. It is intended for the stated addressee(s) only. Access to this email by anyone else is unauthorised. If you are not the intended addressee, any disclosure or copying of the contents of this email or any action taken (or not taken) in reliance on it is unauthorised and is unlawful. If you are not the addressee, please inform the sender immediately and destroy the original message. > The data in this email will have been screened for the presence of computer viruses known by the Company at the time the email was produced. The Company cannot guarantee, however, that the email or any attachments are virus free. Delivery of the email will be at the Client's risk. > Registered in England No. 1815730 > VAT Reg. No. GB425507072 > Registered Office: Woolley Road, Alconbury, Huntingdon, Cambridgeshire PE28 4HS > ********************************************************************** > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From foreightl <@t> gmail.com Wed Jun 19 15:09:18 2013 From: foreightl <@t> gmail.com (Patrick Laurie) Date: Wed Jun 19 15:09:22 2013 Subject: [Histonet] Tissue Tek VIP 3000 In-Reply-To: <1371666437.41983.YahooMailNeo@web163105.mail.bf1.yahoo.com> References: <135069b13312463f90a9987ee100f133@BY2PR07MB074.namprd07.prod.outlook.com> <1371666437.41983.YahooMailNeo@web163105.mail.bf1.yahoo.com> Message-ID: Hi Mike, We have two of the VIP 3000's, they are true workhorses. One of ours is from 1969 and still processing without any issues. Make sure that you do have a company nearby that can work on them. You can still find parts for them, I tend to only get parts that are used though. I don't know if Sakura makes new parts. Good luck! Patrick On Wed, Jun 19, 2013 at 2:27 PM, Rene J Buesa wrote: > According with the model and brand the price is very good. > Why don't you contact Sakura to find about parts for it in existence or > even a maintenance contract with them? > Sakura VIP are top of the line in tissue processing. > Ren? J. > > From: Mike Tighe > To: "histonet@lists.utsouthwestern.edu (histonet@lists.utsouthwestern.edu)" > > Sent: Wednesday, June 19, 2013 2:22 PM > Subject: [Histonet] Tissue Tek VIP 3000 > > > In an internet search I came across a used TissueTek VIP 3000 that is in > "excellent condition". This looks to be an older model and I am wondering > if anyone has any experience with this instrument? Are there any issues > with parts replacement? comes with one year warranty and they are asking > around $8000.00. > > Thanks! > Mike > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plaurie@cellnetix.com From liz <@t> premierlab.com Wed Jun 19 15:08:58 2013 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Wed Jun 19 15:10:55 2013 Subject: [Histonet] Mixing Paraffin Brands In-Reply-To: References: , Message-ID: <14E2C6176416974295479C64A11CB9AE016411AB48F6@SBS2K8.premierlab.local> That's what we do and we do not have any issues with bone, we use paraplast to infiltrate and paraplast extra to embed in. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 881-0763 cell (303) 682-9060 fax liz@premierlab.com Ship to address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Debra Siena [DSiena@statlab.com] Sent: Wednesday, June 19, 2013 12:29 PM To: Lucie Guernsey; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Mixing Paraffin Brands Hi Lucie, Paraplast Plus has DMSO and Paraplast Xtra doesn't. It may be best to infiltrate with the Plus which is softer and then embed with the Paraplast Xtra which is a bit harder. I don't usually recommend mixing them especially if you only have a few bags. Thanks Debbie Siena 800.442.3573 ext. 229 | www.statlab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lucie Guernsey Sent: Wednesday, June 19, 2013 1:01 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Mixing Paraffin Brands Hi, Does anyone know if there's a reason why one shouldn't mix different brands of paraffin? We normally use Fisherbrand's Paraplast Plus (melting point 56 C). We inherited about 8 bags of McCormick Paraplast X-tra (melting point 52 C). Will the different melting points be a problem? If we were to use the McCormick paraffin, the only place it may mix with the Fisherbrand paraffin is in the blocks themselves (as we refill the embedder). But I don't want to compromise the quality of our blocks just to not waste the free paraffin. Or, another option could be that we use the McCormick in the processor and the Fisherbrand in the embedder. Could that cause issues in the blocks as the tissue would be infiltrated with one brand and embedded in another? Maybe I'm over-thinking this...... Many thanks! Lucie Lucie Guernsey UCSD Dept. of Pathology lguernsey@ucsd.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lguernsey <@t> ucsd.edu Wed Jun 19 17:22:00 2013 From: lguernsey <@t> ucsd.edu (Lucie Guernsey) Date: Wed Jun 19 17:22:46 2013 Subject: [Histonet] Mixing Paraffin Brands In-Reply-To: <14E2C6176416974295479C64A11CB9AE016411AB48F6@SBS2K8.premierlab.local> References: <14E2C6176416974295479C64A11CB9AE016411AB48F6@SBS2K8.premierlab.local> Message-ID: Thank you all for your responses! I'm so glad I asked - I didn't realize that plus was softer than xtra. Now I'm definitely interested in trying to embed using the xtra since sometimes I feel like our blocks could be harder. I think I'll follow the majority's advice: infiltrate using Fisher's Paraplast Plus and embed using McCormick's Paraplast X-tra. Thanks again! Lucie Lucie Guernsey UCSD Dept. of Pathology lguernsey@ucsd.edu On Wed, Jun 19, 2013 at 1:08 PM, Elizabeth Chlipala wrote: > That's what we do and we do not have any issues with bone, we use > paraplast to infiltrate and paraplast extra to embed in. > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > Premier Laboratory, LLC > PO Box 18592 > Boulder, CO 80308 > (303) 682-3949 office > (303) 881-0763 cell > (303) 682-9060 fax > liz@premierlab.com > > Ship to address: > > Premier Laboratory, LLC > 1567 Skyway Drive, Unit E > Longmont, CO 80504 > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [ > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Debra Siena [ > DSiena@statlab.com] > Sent: Wednesday, June 19, 2013 12:29 PM > To: Lucie Guernsey; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Mixing Paraffin Brands > > Hi Lucie, > > Paraplast Plus has DMSO and Paraplast Xtra doesn't. It may be best to > infiltrate with the Plus which is softer and then embed with the Paraplast > Xtra which is a bit harder. I don't usually recommend mixing them > especially if you only have a few bags. Thanks > > Debbie Siena > 800.442.3573 ext. 229 | www.statlab.com > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lucie Guernsey > Sent: Wednesday, June 19, 2013 1:01 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Mixing Paraffin Brands > > Hi, > > Does anyone know if there's a reason why one shouldn't mix different > brands of paraffin? We normally use Fisherbrand's Paraplast Plus (melting > point 56 C). We inherited about 8 bags of McCormick Paraplast X-tra > (melting point > 52 C). Will the different melting points be a problem? > > If we were to use the McCormick paraffin, the only place it may mix with > the Fisherbrand paraffin is in the blocks themselves (as we refill the > embedder). But I don't want to compromise the quality of our blocks just to > not waste the free paraffin. > > Or, another option could be that we use the McCormick in the processor and > the Fisherbrand in the embedder. Could that cause issues in the blocks as > the tissue would be infiltrated with one brand and embedded in another? > > Maybe I'm over-thinking this...... > > Many thanks! > Lucie > > Lucie Guernsey > UCSD > Dept. of Pathology > lguernsey@ucsd.edu > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From saby_joseph_a <@t> yahoo.com Wed Jun 19 17:27:47 2013 From: saby_joseph_a <@t> yahoo.com (Joseph Saby) Date: Wed Jun 19 17:27:52 2013 Subject: [Histonet] Processing Guinea Pig In-Reply-To: References: Message-ID: <1371680867.34822.YahooMailNeo@web163003.mail.bf1.yahoo.com> Heather- ? I do not know why, but to properly process guinea pig tissues you need a much more rigorous program than what would work for mice.?It is very easy to over process mouse tissue.? Even rat tissue needs more processing.? Guinea pig tissue need a program designed for processing larger animals/tissues, such as one would use to process dogs or even swine. ? Let me know what programs you have, and I will get back with you with what would work.? ? Joe Saby BA HT NAMSA, Inc. ________________________________ From: Heather Marlatt To: "histonet@lists.utsouthwestern.edu" Sent: Monday, June 17, 2013 10:33 AM Subject: [Histonet] Processing Guinea Pig Hello Histonet! I'm a long time reader first time poster. Does anyone have experience processing guinea pig tissues? I have been processing kidney and heart but it is consistently coming out mushy in the middle. The mouse tissue comes out fine even when processed on the same run. I had the tissue grossed in thinner (2.5mm) thinking that perhaps it was too thick but it didn't seem to help. Also, it has been fixed in 10%NBF for several days. I was just wondering if anyone else had similar problems with guinea pig? I appreciate in advance any advice or tips. Here is the protocol: Formalin 1hr 70% etOH 1hr 95% 1hr 100% 30min 100% 1hr 100% 1hr 100%1hr Clearify 1hr Clearify? 1hr Clearify 1hr Paraffin 1hr paraffin 1hr All under pressure and heat only on the paraffin. Thanks Heather _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Jun 19 18:34:00 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jun 19 18:34:06 2013 Subject: [Histonet] Mixing Paraffin Brands In-Reply-To: References: <1371666267.45338.YahooMailNeo@web163104.mail.bf1.yahoo.com> Message-ID: <1371684840.32300.YahooMailNeo@web163105.mail.bf1.yahoo.com> Use a 65?C MP paraffin from Merk Ren? J. From: "Louro, Pedro" To: Rene J Buesa ; Lucie Guernsey ; histonet@lists.utsouthwestern.edu Sent: Wednesday, June 19, 2013 2:32 PM Subject: RE: [Histonet] Mixing Paraffin Brands Just out of curiosity, what paraffin would people out there recommend using for animal bone joints and turbinates? We are currently using parapalst plus and was thinking of changing to a "harder paraffin" Any thoughts??? Pedro L. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, June 19, 2013 2:24 PM To: Lucie Guernsey; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Mixing Paraffin Brands Each paraffin has some additives to improve either its penetration rate or density to section. Mixing different melting points (MP) paraffin will result in another paraffin with an intermediate MP and the sectioning will be different. Preparing the block with the mixture will probably cause so troubles while sectioning. Why don't you just use them separate? There is no good reason to mix them and the two paraffins you mention are of good quality. Ren? J. From: Lucie Guernsey To: histonet@lists.utsouthwestern.edu Sent: Wednesday, June 19, 2013 2:01 PM Subject: [Histonet] Mixing Paraffin Brands Hi, Does anyone know if there's a reason why one shouldn't mix different brands of paraffin? We normally use Fisherbrand's Paraplast Plus (melting point 56 C). We inherited about 8 bags of McCormick Paraplast X-tra (melting point 52 C). Will the different melting points be a problem? If we were to use the McCormick paraffin, the only place it may mix with the Fisherbrand paraffin is in the blocks themselves (as we refill the embedder). But I don't want to compromise the quality of our blocks just to not waste the free paraffin. Or, another option could be that we use the McCormick in the processor and the Fisherbrand in the embedder. Could that cause issues in the blocks as the tissue would be infiltrated with one brand and embedded in another? Maybe I'm over-thinking this...... Many thanks! Lucie Lucie Guernsey UCSD Dept. of Pathology lguernsey@ucsd.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************************************************************************************** Our Values:? Excellence, Drive, Ownership, Challenge, Teamwork, Respect ******************************************************************************************** LEGAL NOTICE This message is confidential and contains information which may be legally privileged. It is intended for the stated addressee(s) only. Access to this email by anyone else is unauthorised. If you are not the intended addressee, any disclosure or copying of the contents of this email or any action taken (or not taken) in reliance on it is unauthorised and is unlawful. If you are not the addressee, please inform the sender immediately and destroy the original message. The data in this email will have been screened for the presence of computer viruses known by the Company at the time the email was produced. The Company cannot guarantee, however, that the email or any attachments are virus free. Delivery of the email will be at the Client's risk. Registered in England No. 1815730 VAT Reg. No. GB425507072 Registered Office: Woolley Road, Alconbury, Huntingdon, Cambridgeshire PE28 4HS ********************************************************************** From Susan.Walzer <@t> HCAHealthcare.com Thu Jun 20 02:16:16 2013 From: Susan.Walzer <@t> HCAHealthcare.com (Susan.Walzer@HCAHealthcare.com) Date: Thu Jun 20 02:16:26 2013 Subject: [Histonet] Blade Rationing Follow-up In-Reply-To: References: Message-ID: <4BF03F5404EBDE409AF9232DA74B9DED2FA11EF308@FWDCWPMSGCMS09.hca.corpad.net> You can shop all the blades in the world( and we have tried many) but if you care about quality sections the Accu-edges are the only game in town. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Thomas Jasper Sent: Wednesday, June 19, 2013 3:20 PM To: 'Teresa Moore'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Blade Rationing Follow-up You could start with cost comparison of blades (since this whole thing started as a blade issue). Reps are willing to let you demo some before committing and you might save money if everyone likes a less expensive blade. You can do the same for paraffin and slides as well as reagent alcohols and xylene. Again, these are things the manager should be getting after. Does your lab adhere to Lean principles? There may be some cost savings there if you can accomplish tasks more efficiently; get the work done in less time or cutting back on overtime. It's a bit difficult to say as much depends on the nature and scope of your service. All situations are unique - do you send a lot of work out? Could you take it on in-house? Or are you trying to do things in-house that could be sent out and have a cost positive effect? Lots of questions for the manager... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Teresa Moore Sent: Tuesday, June 18, 2013 1:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Blade Rationing Follow-up I really appreciate everyone's constructive comments regarding my post on blade rationing. Lots of you said there are many other ways to cut costs in the lab. I would like to hear some of your suggestions so I can take them back to my manager. I'd like to give her some legitimate alternatives to her proposal. Would like to contribute to solving the problem of cutting costs. Thanks again Teresa Moore, HT _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Deaconess Health System. If you have received this email in error please notify the originator of the message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mucram11 <@t> comcast.net Thu Jun 20 06:10:17 2013 From: mucram11 <@t> comcast.net (Pam Marcum) Date: Thu Jun 20 06:10:46 2013 Subject: [Histonet] Blade Rationing Follow-up In-Reply-To: <4BF03F5404EBDE409AF9232DA74B9DED2FA11EF308@FWDCWPMSGCMS09.hca.corpad.net> Message-ID: <948780997.369305.1371726617974.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> I would disagree as we have used the Thermo Premiers and they are just as good and?we are n ot paying for the name.? I h ave tried just about every new blade used in Histology going back to sharpening the big blades and if you look there are excellent blades out there. We changed from Accu Edge several years ago everyone was upset for a few weeks.??Then I offered them some back after?a few Accu Edge?packages were found ?in my office.? Suddenly ? the staff that could only use Accu Edge refused to use them as they had changed their minds.?So you can find others if you are aware?of quality and price.? There are some bad blades out there that we would not use however; there is no?longer only one game in town for blades. ? Pam ----- Original Message ----- From: "Susan Walzer" To: "Thomas Jasper" , tmoore9k@gmail.com, histonet@lists.utsouthwestern.edu Sent: Thursday, June 20, 2013 2:16:16 AM Subject: RE: [Histonet] Blade Rationing Follow-up You can shop all the blades in the world( and we have tried many) but if you care about quality sections the Accu-edges are the only game in town. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Thomas Jasper Sent: Wednesday, June 19, 2013 3:20 PM To: 'Teresa Moore'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Blade Rationing Follow-up You could start with cost comparison of blades (since this whole thing started as a blade issue). ?Reps are willing to let you demo some before committing and you might save money if everyone likes a less expensive blade. ?You can do the same for paraffin and slides as well as reagent alcohols and xylene. ?Again, these are things the manager should be getting after. ?Does your lab adhere to Lean principles? ?There may be some cost savings there if you can accomplish tasks more efficiently; get the work done in less time or cutting back on overtime. ?It's a bit difficult to say as much depends on the nature and scope of your service. ?All situations are unique - do you send a lot of work out? ?Could you take it on in-house? ?Or are you trying to do things in-house that could be sent out and have a cost positive effect? ?Lots of questions for the manager... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Teresa Moore Sent: Tuesday, June 18, 2013 1:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Blade Rationing Follow-up I really appreciate everyone's constructive comments regarding my post on blade rationing. ?Lots of you said there are many other ways to cut costs in the lab. ?I would like to hear some of your suggestions so I can take them back to my manager. ?I'd like to give her some legitimate alternatives to her proposal. Would like to contribute to solving the problem of cutting costs. Thanks again Teresa Moore, HT _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Deaconess Health System. If you have received this email in error please notify the originator of the message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HornHV <@t> archildrens.org Thu Jun 20 07:08:07 2013 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Thu Jun 20 07:08:17 2013 Subject: [Histonet] Blade Rationing Follow-up In-Reply-To: <948780997.369305.1371726617974.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> References: <4BF03F5404EBDE409AF9232DA74B9DED2FA11EF308@FWDCWPMSGCMS09.hca.corpad.net> <948780997.369305.1371726617974.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Message-ID: <25A4DE08332B19499904459F00AAACB719C357325E@EVS1.archildrens.org> I agree with Pam. The Thermo Premiers are excellent blades. We prefer them. My personal opinion is the Accu-Edge are not as good as they used to be. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children?s Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pam Marcum Sent: Thursday, June 20, 2013 6:10 AM To: Susan Walzer Cc: histonet@lists.utsouthwestern.edu; tmoore9k@gmail.com Subject: Re: [Histonet] Blade Rationing Follow-up I would disagree as we have used the Thermo Premiers and they are just as good and?we are n ot paying for the name.? I h ave tried just about every new blade used in Histology going back to sharpening the big blades and if you look there are excellent blades out there. We changed from Accu Edge several years ago everyone was upset for a few weeks.??Then I offered them some back after?a few Accu Edge?packages were found ?in my office.? Suddenly ? the staff that could only use Accu Edge refused to use them as they had changed their minds.?So you can find others if you are aware?of quality and price.? There are some bad blades out there that we would not use however; there is no?longer only one game in town for blades. ? Pam ----- Original Message ----- From: "Susan Walzer" To: "Thomas Jasper" , tmoore9k@gmail.com, histonet@lists.utsouthwestern.edu Sent: Thursday, June 20, 2013 2:16:16 AM Subject: RE: [Histonet] Blade Rationing Follow-up You can shop all the blades in the world( and we have tried many) but if you care about quality sections the Accu-edges are the only game in town. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Thomas Jasper Sent: Wednesday, June 19, 2013 3:20 PM To: 'Teresa Moore'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Blade Rationing Follow-up You could start with cost comparison of blades (since this whole thing started as a blade issue). ?Reps are willing to let you demo some before committing and you might save money if everyone likes a less expensive blade. ?You can do the same for paraffin and slides as well as reagent alcohols and xylene. ?Again, these are things the manager should be getting after. ?Does your lab adhere to Lean principles? ?There may be some cost savings there if you can accomplish tasks more efficiently; get the work done in less time or cutting back on overtime. ?It's a bit difficult to say as much depends on the nature and scope of your service. ?All situations are unique - do you send a lot of work out? ?Could you take it on in-house? ?Or are you trying to do things in-house that could be sent out and have a cost positive effect? ?Lots of questions for the manager... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Teresa Moore Sent: Tuesday, June 18, 2013 1:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Blade Rationing Follow-up I really appreciate everyone's constructive comments regarding my post on blade rationing. ?Lots of you said there are many other ways to cut costs in the lab. ?I would like to hear some of your suggestions so I can take them back to my manager. ?I'd like to give her some legitimate alternatives to her proposal. Would like to contribute to solving the problem of cutting costs. Thanks again Teresa Moore, HT _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Deaconess Health System. If you have received this email in error please notify the originator of the message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From joelleweaver <@t> hotmail.com Thu Jun 20 09:07:22 2013 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Thu Jun 20 09:07:27 2013 Subject: [Histonet] RE: Her 2 In-Reply-To: References: , , Message-ID: Hi I am just wondering if anyone would be willing to reply and provide their thoughts on the idea of providing correlation studies for labs that don't do FISH in-house for Her 2 correlation ? I will have the FDA approved FISH for Her2 and also perform IHC and possibly ISH ( on Bond platform). Just seeking input....Thanks Joelle Weaver MAOM, HTL (ASCP) QIHC To: joelleweaver@hotmail.com Subject: RE: [Histonet] IHC Tech Competency Checklist From: JMaslanka@stpetes.org Date: Wed, 19 Jun 2013 13:24:03 -0600 Thanks Again Joe Maslanka BS, CT,HT (ASCP) Anatomical Pathology Technical Supervisor St Peter's Hospital,MT 59601 (P)(406) 447-2406 (F)(406)444-2126 Give thanks for ALL things..... "Kindness is the language the blind can see & the deaf can hear- Mark Twain This electronic mail message contains information which is confidential. If you are not the intended recipient, please be aware that any disclosure, photocopying, distribution or use of the contents of the received information is prohibited. If you have received this e-mail in error, please reply to the sender immediately and permanently delete this message and all copies of it. Thank you. From rjbuesa <@t> yahoo.com Thu Jun 20 09:32:26 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jun 20 09:32:30 2013 Subject: [Histonet] RE: Her 2 In-Reply-To: References: , , Message-ID: <1371738746.24954.YahooMailNeo@web163103.mail.bf1.yahoo.com> This is a very god idea, but I think you should first review the literature because I am quite sure that this type of study has already been done. I suggest you to contact CAP about this issue before embarking on such a study that will have to have an experimental design including the sample size and all the variables that could affect it such as fixatives and fixation times; processing protocols and reagents as well as all the different clones that can be used. Also all the variables affecting the FISH results. This will also be an extremely costly project so you will have to seek a sponsor or get written consent from your employer to avoid problems down the road. This is why I think that you should review first the literature. Ren? J. From: joelle weaver To: "histonet@lists.utsouthwestern.edu" Sent: Thursday, June 20, 2013 10:07 AM Subject: [Histonet] RE: Her 2 Hi I am just wondering if anyone would be willing to reply and provide their thoughts on the idea of providing correlation studies for labs that don't do FISH in-house for Her 2 correlation ? I will have the FDA approved FISH for Her2 and also perform IHC and possibly ISH ( on Bond platform). Just seeking input....Thanks Joelle Weaver MAOM, HTL (ASCP) QIHC To: joelleweaver@hotmail.com Subject: RE: [Histonet] IHC Tech Competency Checklist From: JMaslanka@stpetes.org Date: Wed, 19 Jun 2013 13:24:03 -0600 Thanks Again Joe Maslanka BS, CT,HT (ASCP) Anatomical Pathology Technical Supervisor St Peter's Hospital,MT 59601 (P)(406) 447-2406 (F)(406)444-2126? ? ? ? ? ? ? ? ? Give thanks for ALL things..... "Kindness is the language the blind can see & the deaf can hear- Mark Twain This electronic mail message contains information which is confidential. If you are not the intended recipient, please be aware that any disclosure, photocopying, distribution or use of the contents of the received information is prohibited. If you have received this e-mail in error, please reply to the sender immediately and permanently delete this message and all copies of it. Thank you. ??? ??? ??? ? ??? ??? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mroark <@t> sfmc.net Thu Jun 20 10:24:40 2013 From: mroark <@t> sfmc.net (Matthew Roark) Date: Thu Jun 20 10:25:02 2013 Subject: [Histonet] Validation Protocols Message-ID: <003801ce6dca$48de1b60$da9a5220$@net> Would anybody be willing to share their protocol/policy for the validation of a new IHC stainer and/or tissue processor? Thanks!! Matthew Roark- HT/HTL(ASCP)CM Histology Specialist Saint Francis Medical Center 211 Saint Francis Drive Cape Girardeau, MO 63703 573-331-5267 mroark@sfmc.net http://www.sfmc.net From joelleweaver <@t> hotmail.com Thu Jun 20 10:28:53 2013 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Thu Jun 20 10:28:58 2013 Subject: [Histonet] RE: Her 2 In-Reply-To: <1371738746.24954.YahooMailNeo@web163103.mail.bf1.yahoo.com> References: , , , , , , <1371738746.24954.YahooMailNeo@web163103.mail.bf1.yahoo.com> Message-ID: Not looking to publish anything or do any study. I will be doing the tests already. Thanks for the suggestions. Joelle Weaver MAOM, HTL (ASCP) QIHC Date: Thu, 20 Jun 2013 07:32:26 -0700 From: rjbuesa@yahoo.com Subject: Re: [Histonet] RE: Her 2 To: joelleweaver@hotmail.com; histonet@lists.utsouthwestern.edu This is a very god idea, but I think you should first review the literature because I am quite sure that this type of study has already been done. I suggest you to contact CAP about this issue before embarking on such a study that will have to have an experimental design including the sample size and all the variables that could affect it such as fixatives and fixation times; processing protocols and reagents as well as all the different clones that can be used. Also all the variables affecting the FISH results. This will also be an extremely costly project so you will have to seek a sponsor or get written consent from your employer to avoid problems down the road. This is why I think that you should review first the literature. Ren? J. From: joelle weaver To: "histonet@lists.utsouthwestern.edu" Sent: Thursday, June 20, 2013 10:07 AM Subject: [Histonet] RE: Her 2 Hi I am just wondering if anyone would be willing to reply and provide their thoughts on the idea of providing correlation studies for labs that don't do FISH in-house for Her 2 correlation ? I will have the FDA approved FISH for Her2 and also perform IHC and possibly ISH ( on Bond platform). Just seeking input....Thanks Joelle Weaver MAOM, HTL (ASCP) QIHC To: joelleweaver@hotmail.com Subject: RE: [Histonet] IHC Tech Competency Checklist From: JMaslanka@stpetes.org Date: Wed, 19 Jun 2013 13:24:03 -0600 Thanks Again Joe Maslanka BS, CT,HT (ASCP) Anatomical Pathology Technical Supervisor St Peter's Hospital,MT 59601 (P)(406) 447-2406 (F)(406)444-2126 Give thanks for ALL things..... "Kindness is the language the blind can see & the deaf can hear- Mark Twain This electronic mail message contains information which is confidential. If you are not the intended recipient, please be aware that any disclosure, photocopying, distribution or use of the contents of the received information is prohibited. If you have received this e-mail in error, please reply to the sender immediately and permanently delete this message and all copies of it. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Thu Jun 20 10:33:50 2013 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Thu Jun 20 10:33:55 2013 Subject: [Histonet] RE: Her 2 In-Reply-To: <1371738746.24954.YahooMailNeo@web163103.mail.bf1.yahoo.com> References: , , , , , , , <1371738746.24954.YahooMailNeo@web163103.mail.bf1.yahoo.com> Message-ID: I have looked at a substantial amount of literature already, just to become more educated, generally , including the orignal FDA study. I am aware of the CAP/ASCO releases , regulations, updates and resources for laboratories. Any other "must reads" on this topic anyone can recommend? In no way , was I suggesting such an undertaking, just seeing if I could assist anyone. I know about the CAP lab list, not even trying to get on that. Joelle Weaver MAOM, HTL (ASCP) QIHC > Date: Thu, 20 Jun 2013 07:32:26 -0700 > From: rjbuesa@yahoo.com > To: joelleweaver@hotmail.com; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] RE: Her 2 > CC: > > This is a very god idea, but I think you should first review the literature because I am quite sure that this type of study has already been done. > I suggest you to contact CAP about this issue before embarking on such a study that will have to have an experimental design including the sample size and all the variables that could affect it such as fixatives and fixation times; processing protocols and reagents as well as all the different clones that can be used. Also all the variables affecting the FISH results. This will also be an extremely costly project so you will have to seek a sponsor or get written consent from your employer to avoid problems down the road. > This is why I think that you should review first the literature. > Ren? J. > > From: joelle weaver > To: "histonet@lists.utsouthwestern.edu" > Sent: Thursday, June 20, 2013 10:07 AM > Subject: [Histonet] RE: Her 2 > > > Hi > I am just wondering if anyone would be willing to reply and provide their thoughts on the idea of providing correlation studies for labs that don't do FISH in-house for Her 2 correlation ? > I will have the FDA approved FISH for Her2 and also perform IHC and possibly ISH ( on Bond platform). > > Just seeking input....Thanks > > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > To: joelleweaver@hotmail.com > Subject: RE: [Histonet] IHC Tech Competency Checklist > From: JMaslanka@stpetes.org > Date: Wed, 19 Jun 2013 13:24:03 -0600 > > Thanks Again > > > > > > Joe Maslanka BS, CT,HT (ASCP) > > Anatomical Pathology Technical Supervisor > > St Peter's Hospital,MT 59601 > > (P)(406) 447-2406 > > (F)(406)444-2126 > > > > > Give thanks for ALL things..... > > "Kindness is the language the blind can see & the deaf can hear- > Mark Twain > > > > > > > > This electronic mail message contains information which is confidential. > If you are not the intended recipient, please be aware that any disclosure, > photocopying, distribution or use of the contents of the received information > is prohibited. If you have received this e-mail in error, please reply > to the sender immediately and permanently delete this message and all copies > of it. Thank you. _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From joelleweaver <@t> hotmail.com Thu Jun 20 10:36:45 2013 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Thu Jun 20 10:36:50 2013 Subject: [Histonet] RE: Her 2 In-Reply-To: <9C8F910F72893643B3C3793C3D67132B013A54C6@PATHOLOGYSERVER.pathologyarts.local> References: , , , , , , , <9C8F910F72893643B3C3793C3D67132B013A54C6@PATHOLOGYSERVER.pathologyarts.local> Message-ID: According to my Lecia consultant I can run a concentrated AB and also ISH. I will be working it up in a few weeks. I will also do manual FISH PathVyson, Her 2. I was not looking for participants for some kind of study, just seeing if when I had all 3, I could help other labs. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: c.tague@Pathologyarts.com > To: joelleweaver@hotmail.com > Subject: RE: [Histonet] RE: Her 2 > Date: Thu, 20 Jun 2013 14:56:09 +0000 > > Hi Joelle, > > Sorry but I'm not able to add to your correlation study as we are not currently providing any Her2 on site. That's actually why I'm writing, I'm trying to learn a little about this to provide that option here at my lab. I too have the Bond platform, how are you running Her2 on that system. I just assumed it was a FISH or ISH test, are you running them on the bond? I'd like to be able to offer them as well but Leica does not have anything for Her2. Any input will be greatly appreciated, > > Have a great day, > > Curt > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelle weaver > Sent: Thursday, June 20, 2013 7:07 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: Her 2 > > Hi > I am just wondering if anyone would be willing to reply and provide their thoughts on the idea of providing correlation studies for labs that don't do FISH in-house for Her 2 correlation ? > I will have the FDA approved FISH for Her2 and also perform IHC and possibly ISH ( on Bond platform). > > Just seeking input....Thanks > > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > To: joelleweaver@hotmail.com > Subject: RE: [Histonet] IHC Tech Competency Checklist > From: JMaslanka@stpetes.org > Date: Wed, 19 Jun 2013 13:24:03 -0600 > > Thanks Again > > > > > > Joe Maslanka BS, CT,HT (ASCP) > > Anatomical Pathology Technical Supervisor > > St Peter's Hospital,MT 59601 > > (P)(406) 447-2406 > > (F)(406)444-2126 > > > > > Give thanks for ALL things..... > > "Kindness is the language the blind can see & the deaf can hear- Mark Twain > > > > > > > > This electronic mail message contains information which is confidential. > If you are not the intended recipient, please be aware that any disclosure, photocopying, distribution or use of the contents of the received information is prohibited. If you have received this e-mail in error, please reply to the sender immediately and permanently delete this message and all copies > of it. Thank you. _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Jun 20 10:57:28 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jun 20 10:57:32 2013 Subject: [Histonet] RE: Her 2 In-Reply-To: References: , , , , , , <1371738746.24954.YahooMailNeo@web163103.mail.bf1.yahoo.com> Message-ID: <1371743848.44164.YahooMailNeo@web163104.mail.bf1.yahoo.com> Then I misinterpreted your original posting because you wrote "correlation studies for labs that don't do FISH" for me it meant that you were going to share your results with other labs and in that case you needed to do what I recommended. Ren? J. From: joelle weaver To: Rene J Buesa ; "histonet@lists.utsouthwestern.edu" Sent: Thursday, June 20, 2013 11:28 AM Subject: RE: [Histonet] RE: Her 2 Not looking to publish anything or do any study. I will be doing the tests already. ?Thanks for the suggestions. Joelle Weaver MAOM, HTL (ASCP) QIHC ? Date: Thu, 20 Jun 2013 07:32:26 -0700 From: rjbuesa@yahoo.com Subject: Re: [Histonet] RE: Her 2 To: joelleweaver@hotmail.com; histonet@lists.utsouthwestern.edu This is a very god idea, but I think you should first review the literature because I am quite sure that this type of study has already been done. I suggest you to contact CAP about this issue before embarking on such a study that will have to have an experimental design including the sample size and all the variables that could affect it such as fixatives and fixation times; processing protocols and reagents as well as all the different clones that can be used. Also all the variables affecting the FISH results. This will also be an extremely costly project so you will have to seek a sponsor or get written consent from your employer to avoid problems down the road. This is why I think that you should review first the literature. Ren? J. From: joelle weaver To: "histonet@lists.utsouthwestern.edu" Sent: Thursday, June 20, 2013 10:07 AM Subject: [Histonet] RE: Her 2 Hi I am just wondering if anyone would be willing to reply and provide their thoughts on the idea of providing correlation studies for labs that don't do FISH in-house for Her 2 correlation ? I will have the FDA approved FISH for Her2 and also perform IHC and possibly ISH ( on Bond platform). Just seeking input....Thanks Joelle Weaver MAOM, HTL (ASCP) QIHC To: joelleweaver@hotmail.com Subject: RE: [Histonet] IHC Tech Competency Checklist From: JMaslanka@stpetes.org Date: Wed, 19 Jun 2013 13:24:03 -0600 Thanks Again Joe Maslanka BS, CT,HT (ASCP) Anatomical Pathology Technical Supervisor St Peter's Hospital,MT 59601 (P)(406) 447-2406 (F)(406)444-2126? ? ? ? ? ? ? ? ? Give thanks for ALL things..... "Kindness is the language the blind can see & the deaf can hear- Mark Twain This electronic mail message contains information which is confidential. If you are not the intended recipient, please be aware that any disclosure, photocopying, distribution or use of the contents of the received information is prohibited. If you have received this e-mail in error, please reply to the sender immediately and permanently delete this message and all copies of it. Thank you. ??? ??? ??? ? ??? ??? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BMolinari <@t> texasheart.org Thu Jun 20 11:02:47 2013 From: BMolinari <@t> texasheart.org (Molinari, Betsy) Date: Thu Jun 20 11:02:53 2013 Subject: [Histonet] Karnovsky's In-Reply-To: <1371666041.21504.YahooMailNeo@web163106.mail.bf1.yahoo.com> References: <1371666041.21504.YahooMailNeo@web163106.mail.bf1.yahoo.com> Message-ID: Rene, Thank you for your response. It is a whole heart. We have documented the arrival condition and took pictures. I took a small piece and processed it last night and will be cutting it in a few minutes. I am pretty sure the results will be sub-optimal. This will be for documentation. Thanks again, Betsy Betsy Molinari Senior Histology Research Technician 832-355-6524 | BMolinari@texasheart.org | www.texasheart.org 6770 Bertner Ave., MC 1-283, Houston, TX 77030 [Texas Heart Institute][THI News] [THI on Facebook] [THI on Flicker] [THI on Google] [THI on Pinterest] [THI on Twitter] [THI on You Tube] Confidentiality Notice: This message may be confidential and/or privileged. If you are not the intended recipient you may not review, disseminate or copy this e-mail, its contents and/or any attachments. Please immediately notify the sender If you have received this e-mail in error and delete it from your computer system. From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: Wednesday, June 19, 2013 1:21 PM To: Molinari, Betsy; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Karnovsky's Are you referring to a whole heart? If that is the case I think the fixation of the tissue will be very poor because Karnovsky's penetrate very slowly. I do not think it will be worth the effort and materials to process this tissue. Ren? J. From: "Molinari, Betsy" > To: "Histonet@lists.utsouthwestern.edu" > Sent: Wednesday, June 19, 2013 1:32 PM Subject: [Histonet] Karnovsky's Hi, I received a heart that was fixed in 2% Karnovsky?s. I was told it was initially kept under refrigeration but then stored at RT. I do not know how long the heart has been stored at RT. By the look of the tissue I would say quite a while. Is this tissue compromised in any way? Should I remove it from the Karnovsky?s and store in in something else? This heart is to undergo paraffin processing. Thanks for any input. Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave MC1-283 Houston,TX 77030 832-355-6524 (lab) 832-355-6812 (fax) Betsy Molinari Senior Histology Research Technician 832-355-6524 | BMolinari@texasheart.org> | www.texasheart.org 6770 Bertner Ave., MC 1-283, Houston, TX 77030 [Texas Heart Institute][THI News] [THI on Facebook] [THI on Flicker] [THI on Google] [THI on Pinterest] [THI on Twitter] [THI on You Tube] Confidentiality Notice: This message may be confidential and/or privileged. If you are not the intended recipient you may not review, disseminate or copy this e-mail, its contents and/or any attachments. Please immediately notify the sender If you have received this e-mail in error and delete it from your computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sdysart <@t> mirnarx.com Thu Jun 20 11:13:16 2013 From: sdysart <@t> mirnarx.com (Sarah Dysart) Date: Thu Jun 20 11:13:23 2013 Subject: [Histonet] Cholesterol Message-ID: <793f2461053a4e4f82ad7ee536857cb2@BLUPR07MB001.namprd07.prod.outlook.com> Has anyone ever done work with cholesterol receptors? Specifically APoB and E? Wondering if there is a specific IHC or special that will stain for these? I know Oil Red would stain all the fat, but they are wanting more of the affinity of each cell to uptake the cholesterol (ie the receptors). Can you get as specific as APoB or do you have to stay general as in the LDL type antibodies? Just throwing it out there to see if there is any insight while I google my day away on the subject =) Thanks in advance, and happy Thursday!! Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 From TJohnson <@t> gnf.org Thu Jun 20 12:18:16 2013 From: TJohnson <@t> gnf.org (Teri Johnson) Date: Thu Jun 20 12:18:22 2013 Subject: [Histonet] Thermo Scientific ClearVue Coverslipper Message-ID: <9F3CFEE76E51B64991C7485270890B4049784954@EX4.lj.gnf.org> Dear colleagues, If any of you have experience using the Thermo ClearVue Coverslipper in your lab, would you please let me know how the instrument is working in your hands? I have experience already with a wide variety of automatic glass coverslippers but have heard virtually nothing about this unit yet. Feel free to email me directly. Best wishes, Teri Johnson Manager, Histology GNF - San Diego, CA 858-332-4752 From Lisa.White3 <@t> va.gov Thu Jun 20 13:23:06 2013 From: Lisa.White3 <@t> va.gov (White, Lisa M.) Date: Thu Jun 20 13:23:40 2013 Subject: [Histonet] Fatty Fixation Message-ID: <2B2ECF33934F5D4996D8BE03EFDF397607D89EB2@VHAV09MSGA3.v09.med.va.gov> Does anyone have a method they will share to fix fatty specimens? Does anyone utilize a stir plate? Any help greatly appreciated. We currently use Alcoholic Formalin but the results are not reliable. Lisa White HT(ASCP) Supervisory HT James H. Quillen VAMC Corner of Veterans Way and Lamont VAMC Warehouse BLDG. 205 PO Box 4000 PLMS 113 Mountain Home, TN 37684 423-979-3567 423-979-3401 fax From hymclab.hymclab <@t> ministryhealth.org Thu Jun 20 15:02:37 2013 From: hymclab.hymclab <@t> ministryhealth.org (hymclab) Date: Thu Jun 20 15:02:56 2013 Subject: [Histonet] Blade Rationing Follow-up In-Reply-To: <948780997.369305.1371726617974.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> References: <4BF03F5404EBDE409AF9232DA74B9DED2FA11EF308@FWDCWPMSGCMS09.hca.corpad.net> <948780997.369305.1371726617974.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Message-ID: I agree with you Pam. We changed from Accu Edge to the Thermo Premiers several years ago. The Thermo Premiers gives us the opportunity to cut more blocks than the Accu Edge. We love them!!! Dawn D. Schneider, HT(ASCP) Lead HT Howard Young Medical Center 240 Maple Ave. Woodruff, WI 54558 715-356-8174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pam Marcum Sent: Thursday, June 20, 2013 6:10 AM To: Susan Walzer Cc: histonet@lists.utsouthwestern.edu; tmoore9k@gmail.com Subject: Re: [Histonet] Blade Rationing Follow-up I would disagree as we have used the Thermo Premiers and they are just as good and we are n ot paying for the name. I h ave tried just about every new blade used in Histology going back to sharpening the big blades and if you look there are excellent blades out there. We changed from Accu Edge several years ago everyone was upset for a few weeks. Then I offered them some back after a few Accu Edge packages were found in my office. Suddenly the staff that could only use Accu Edge refused to use them as they had changed their minds. So you can find others if you are aware of quality and price. There are some bad blades out there that we would not use however; there is no longer only one game in town for blades. Pam ----- Original Message ----- From: "Susan Walzer" To: "Thomas Jasper" , tmoore9k@gmail.com, histonet@lists.utsouthwestern.edu Sent: Thursday, June 20, 2013 2:16:16 AM Subject: RE: [Histonet] Blade Rationing Follow-up You can shop all the blades in the world( and we have tried many) but if you care about quality sections the Accu-edges are the only game in town. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Thomas Jasper Sent: Wednesday, June 19, 2013 3:20 PM To: 'Teresa Moore'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Blade Rationing Follow-up You could start with cost comparison of blades (since this whole thing started as a blade issue). Reps are willing to let you demo some before committing and you might save money if everyone likes a less expensive blade. You can do the same for paraffin and slides as well as reagent alcohols and xylene. Again, these are things the manager should be getting after. Does your lab adhere to Lean principles? There may be some cost savings there if you can accomplish tasks more efficiently; get the work done in less time or cutting back on overtime. It's a bit difficult to say as much depends on the nature and scope of your service. All situations are unique - do you send a lot of work out? Could you take it on in-house? Or are you trying to do things in-house that could be sent out and have a cost positive effect? Lots of questions for the manager... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Teresa Moore Sent: Tuesday, June 18, 2013 1:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Blade Rationing Follow-up I really appreciate everyone's constructive comments regarding my post on blade rationing. Lots of you said there are many other ways to cut costs in the lab. I would like to hear some of your suggestions so I can take them back to my manager. I'd like to give her some legitimate alternatives to her proposal. Would like to contribute to solving the problem of cutting costs. Thanks again Teresa Moore, HT _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Deaconess Health System. If you have received this email in error please notify the originator of the message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. From ahorvath <@t> cogipath.com Thu Jun 20 15:15:45 2013 From: ahorvath <@t> cogipath.com (Andrew Horvath) Date: Thu Jun 20 15:16:08 2013 Subject: [Histonet] Validation Protocols (ANP.23120) In-Reply-To: <003801ce6dca$48de1b60$da9a5220$@net> References: <003801ce6dca$48de1b60$da9a5220$@net> Message-ID: The following is a copy of validation protocol I received from CAP... Dear Andrew, Here is the information for ANP.23120: To validate a new processing schedule the user should run tissue samples in duplicate. Side by side samples of the tissue that have been fixed for the same amount of time and are of the same size and thickness. Reagents on the processor should be of the same age and quality, i.e. all fresh reagents. Process, embed cut and stain slides (at the same time). The quality of the blocks should be evaluated on tissue quality I.e. Firmness, ease of cutting etc. The slides can be blind evaluated with the pathologist not knowing which processing schedule was used. Grade on quality of section and staining quality. The new process schedule must be of equal or better quality before put into use. This method can also be used when using the same processing schedule but setting up a new processor of the same model or when setting up a new brand of processor. Multiple common tissue types should be evaluated. Fake biopsies can be created so that patient tissues are not compromised. If you are changing a specific program on a single processor, like you are adjusting the ties or reagents on a breast tissue program. You run the first set of samples on the processor using the routine program and the run the duplicate set of tissues on the same processor using the new program to be validated. When validating programs for a new processor (especially when the new processor is a new brand or different model) you run the first set of tissues on the routine program on the old processor and the second set using the same program on the new processor. I hope this information has been helpful. Sincerely, Arlene Clancy MBA MT(ASCP)SC Technical Team, CAP Accreditation Programs College of American Pathologists Andrew Horvath, MBA, MA Interim Operations Manager 303-770-4848 (O) 303-770-6641 (fax) Colorado GI Pathology 7346 S. Alton Way Suite 10E Centennial, CO 80112 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Matthew Roark Sent: Thursday, June 20, 2013 9:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Validation Protocols Would anybody be willing to share their protocol/policy for the validation of a new IHC stainer and/or tissue processor? Thanks!! Matthew Roark- HT/HTL(ASCP)CM Histology Specialist Saint Francis Medical Center 211 Saint Francis Drive Cape Girardeau, MO 63703 573-331-5267 mroark@sfmc.net http://www.sfmc.net _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brannon <@t> alliedsearchpartners.com Thu Jun 20 15:37:14 2013 From: brannon <@t> alliedsearchpartners.com (Brannon Owens) Date: Thu Jun 20 15:37:27 2013 Subject: [Histonet] Histology leadership role in Long Beach, CA Message-ID: ASCP certified HT or HTL needed for full time permanent position. M-F day shift. Contact for full job description or to apply. Brannon Owens Recruitment Manager Allied Search Partners LinkedIn: http://www.linkedin.com/pub/brannon-owens/28/528/823 http://www.alliedsearchpartners.com T: 888.388.7571 ext. 106 F: 888.388.7572 From rjbuesa <@t> yahoo.com Thu Jun 20 15:38:08 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jun 20 15:38:17 2013 Subject: [Histonet] Fatty Fixation In-Reply-To: <2B2ECF33934F5D4996D8BE03EFDF397607D89EB2@VHAV09MSGA3.v09.med.va.gov> References: <2B2ECF33934F5D4996D8BE03EFDF397607D89EB2@VHAV09MSGA3.v09.med.va.gov> Message-ID: <1371760688.29681.YahooMailNeo@web163101.mail.bf1.yahoo.com> The answer is time, time, time (>36h) Ren? J. From: "White, Lisa M." To: histonet@lists.utsouthwestern.edu Sent: Thursday, June 20, 2013 2:23 PM Subject: [Histonet] Fatty Fixation Does anyone have a method they will share to fix fatty specimens?? Does anyone utilize a stir plate?? Any help greatly appreciated.? We currently use Alcoholic Formalin but the results are not reliable. Lisa White HT(ASCP) Supervisory HT James H. Quillen VAMC Corner of Veterans Way and Lamont VAMC Warehouse BLDG. 205 PO Box 4000 PLMS 113 Mountain Home, TN 37684 423-979-3567 423-979-3401 fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From awatanabe <@t> tgen.org Thu Jun 20 20:27:52 2013 From: awatanabe <@t> tgen.org (awatanabe@tgen.org) Date: Thu Jun 20 20:28:08 2013 Subject: [Histonet] Less costly IHC optimization instruments Message-ID: Hi everyone, I'm looking into ways of doing my IHC optimizations for less up front costs on reagents instead of using my big Ventana instrument. I've seen this product advertised and wondered if anyone has one or has used one. If so can you give me some pros and cons of the instrument? I appreciate any information you can provide. IQ Kinetic Slide Stainer http://biocare.net/products/instrumentation/iq-kinetic-slide-stainer Aprill Watanabe, B.S. Laboratory Coordinator The Dorrance Clinical Laboratory at TGen, Histopathology Laboratory Integrated Cancer Genomics Division Macromolecular Analyte Processing Center (MAPC) Translational Genomics Research Institute (TGen) 445 North 5th Street Phoenix, AZ 85004 Office: 602-343-8822 DCL Lab: 602-343-8796 Fax: 602-343-8717 Cell: 602-481-8654 email: awatanabe@tgen.org website: www.tgen.org From max_histo_00 <@t> yahoo.it Fri Jun 21 00:17:12 2013 From: max_histo_00 <@t> yahoo.it (Massimo) Date: Fri Jun 21 00:17:26 2013 Subject: [Histonet] Fatty Fixation In-Reply-To: <1371760688.29681.YahooMailNeo@web163101.mail.bf1.yahoo.com> References: <2B2ECF33934F5D4996D8BE03EFDF397607D89EB2@VHAV09MSGA3.v09.med.va.gov> <1371760688.29681.YahooMailNeo@web163101.mail.bf1.yahoo.com> Message-ID: <1371791832.84038.YahooMailNeo@web172005.mail.ir2.yahoo.com> You can fix in Flemming's Liquid or, better in an aqueous solution of Osmic Acid at 1% for 24 hours. The Osmic acid has also the property to blacken fats. Kind Regards, Massimo Tosi ________________________________ Da: Rene J Buesa A: "White, Lisa M." ; "histonet@lists.utsouthwestern.edu" Inviato: Gioved? 20 Giugno 2013 22:38 Oggetto: Re: [Histonet] Fatty Fixation The answer is time, time, time (>36h) Ren? J. From: "White, Lisa M." To: histonet@lists.utsouthwestern.edu Sent: Thursday, June 20, 2013 2:23 PM Subject: [Histonet] Fatty Fixation Does anyone have a method they will share to fix fatty specimens?? Does anyone utilize a stir plate?? Any help greatly appreciated.? We currently use Alcoholic Formalin but the results are not reliable. Lisa White HT(ASCP) Supervisory HT James H. Quillen VAMC Corner of Veterans Way and Lamont VAMC Warehouse BLDG. 205 PO Box 4000 PLMS 113 Mountain Home, TN 37684 423-979-3567 423-979-3401 fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Fri Jun 21 06:49:16 2013 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Fri Jun 21 06:49:23 2013 Subject: [Histonet] New FT Histology Position In-Reply-To: <9C8F910F72893643B3C3793C3D67132B013A566B@PATHOLOGYSERVER.pathologyarts.local> References: , , , , , , , <9C8F910F72893643B3C3793C3D67132B013A54C6@PATHOLOGYSERVER.pathologyarts.local>, , <9C8F910F72893643B3C3793C3D67132B013A55E7@PATHOLOGYSERVER.pathologyarts.local>, , <9C8F910F72893643B3C3793C3D67132B013A566B@PATHOLOGYSERVER.pathologyarts.local> Message-ID: PGXL Laboratories, located in Louisville, KY, is seeking a HT (ASCP) or HTL (ASCP) or equivalent for its newly created histology laboratory. This is a unique opportunity for a highly motivated, service oriented individual. The ideal candidate has a minimum of one year experience under the supervision of a board certified pathologist and experience in manual Histochemistry, automated Immunohistochemistry, automated In-situ Hybridization and manual Fluorescent In-situ Hybridization, and/or Cytogenetics experience. Grossing experience of small tissue specimens is also desired. Interested candidates should send their resume to HR@pgxlab.com. Joelle Weaver MAOM, HTL (ASCP) QIHC From: c.tague@Pathologyarts.com To: joelleweaver@hotmail.com Subject: RE: [Histonet] RE: Her 2 Date: Thu, 20 Jun 2013 16:51:48 +0000 Thank you kindly, I will try to remember to follow up, I?d like to be able to offer the same test but, unlike you, I don?t have the QIHC? I?m a little green and novice in the IHC world, still learning, but I?ll get there. Thank you again for offering the help, Curt From: joelle weaver [mailto:joelleweaver@hotmail.com] Sent: Thursday, June 20, 2013 9:26 AM To: Curt Subject: RE: [Histonet] RE: Her 2 I guess my fault, how I worded it was confusing, though I didn't mean it to be. Keep in contact about the Her 2, if I can help in anyway let me know. I have a concentrate, I will validate probably like an ASR Joelle Weaver MAOM, HTL (ASCP) QIHC From: c.tague@Pathologyarts.com To: joelleweaver@hotmail.com Subject: RE: [Histonet] RE: Her 2 Date: Thu, 20 Jun 2013 16:10:43 +0000 Gotch ya, sorry for the misunderstanding. Curt From: joelle weaver [mailto:joelleweaver@hotmail.com] Sent: Thursday, June 20, 2013 8:37 AM To: Curt Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Her 2 According to my Lecia consultant I can run a concentrated AB and also ISH. I will be working it up in a few weeks. I will also do manual FISH PathVyson, Her 2. I was not looking for participants for some kind of study, just seeing if when I had all 3, I could help other labs. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: c.tague@Pathologyarts.com > To: joelleweaver@hotmail.com > Subject: RE: [Histonet] RE: Her 2 > Date: Thu, 20 Jun 2013 14:56:09 +0000 > > Hi Joelle, > > Sorry but I'm not able to add to your correlation study as we are not currently providing any Her2 on site. That's actually why I'm writing, I'm trying to learn a little about this to provide that option here at my lab. I too have the Bond platform, how are you running Her2 on that system. I just assumed it was a FISH or ISH test, are you running them on the bond? I'd like to be able to offer them as well but Leica does not have anything for Her2. Any input will be greatly appreciated, > > Have a great day, > > Curt > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelle weaver > Sent: Thursday, June 20, 2013 7:07 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: Her 2 > > Hi > I am just wondering if anyone would be willing to reply and provide their thoughts on the idea of providing correlation studies for labs that don't do FISH in-house for Her 2 correlation ? > I will have the FDA approved FISH for Her2 and also perform IHC and possibly ISH ( on Bond platform). > > Just seeking input....Thanks > > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > To: joelleweaver@hotmail.com > Subject: RE: [Histonet] IHC Tech Competency Checklist > From: JMaslanka@stpetes.org > Date: Wed, 19 Jun 2013 13:24:03 -0600 > > Thanks Again > > > > > > Joe Maslanka BS, CT,HT (ASCP) > > Anatomical Pathology Technical Supervisor > > St Peter's Hospital,MT 59601 > > (P)(406) 447-2406 > > (F)(406)444-2126 > > > > > Give thanks for ALL things..... > > "Kindness is the language the blind can see & the deaf can hear- Mark Twain > > > > > > > > This electronic mail message contains information which is confidential. > If you are not the intended recipient, please be aware that any disclosure, photocopying, distribution or use of the contents of the received information is prohibited. If you have received this e-mail in error, please reply to the sender immediately and permanently delete this message and all copies > of it. Thank you. _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SGoodacre <@t> chw.org Fri Jun 21 08:12:28 2013 From: SGoodacre <@t> chw.org (Goodacre, Suzanne) Date: Fri Jun 21 08:12:36 2013 Subject: [Histonet] CMV positive wet tissue Message-ID: <1FAD53D23463274AAA30BB5868F4DA9B02AB92@C1XCHPWS12.chwi.chswi.org> Hello, I am in the process of validating CMV ISH on the BOND instrument from Leica for our Histology lab. Currently all of the tissue that we have to use in the validation is negative for CMV and was processed by the VIP 5 processor. Moving forward, the lab we will be using the Peloris processor. For a complete validation, it is necessary for me to use unprocessed formalin fixed tissue that is positive for the CMV virus. We refer to it as "wet" tissue or without processing. Does anyone have any positive tissue that you would be willing to share? It would be greatly appreciated. Thank you. Suzanne From akwong22 <@t> alumni.uwo.ca Fri Jun 21 08:17:50 2013 From: akwong22 <@t> alumni.uwo.ca (Andrea Kwong) Date: Fri Jun 21 08:18:00 2013 Subject: [Histonet] NonSpecific Staining Throughout Frozen Tissue Message-ID: I am using an anti-Sox9 rabbit polyclonal antibody to stain frozen OCT-embedded porcine heart valve tissue, but am getting non-specific staining throughout my sections. I do not think it is something to do directly with the primary antibody because I have been getting this nonspecific staining even on sections with no primary and IgG control and with another antibody (anti-SMA) that we have previously used successfully on frozen porcine tissue. My protocol is as follows: Briefly: 1) Warm slides at room temperature for 20 min 2) Fix in acetone 10 min 3) 3x3min PBS/Tween Wash 4) Peroxidase Block 10 min (3% H2O2 in Methanol) 5) 2x3min PBS/Tween Wash 6) 45 min Horse Serum Block (Vectastain Elite Kit) 7) 1 hour Primary Antibody 8) 4x3min PBS/Tween Wash 9) 30 min Secondary Antibody (Vectastain Elite Kit) 10) 4x3min PBS/Tween Wash 11) 30 min Vectastain ABC (Vectastain Elite Kit) 12) 2x3min PBS/Tween Wash 13) 5 min Vector Nova Red 14) 5 min dH2O 15) Hematoxylin Counterstain 16) Graded alcohol dehydration to xylene 17) Mount with Krystalon I previously thought it may be an issue with endogenous biotin, but I did a run without serum block, primary and secondary... but there was no staining. I'm thinking it may be an issue with fixation affecting secondary, but I'm not sure what to change and was hoping for a second opinion. Thanks for the help From JMaslanka <@t> stpetes.org Fri Jun 21 10:49:13 2013 From: JMaslanka <@t> stpetes.org (JMaslanka@stpetes.org) Date: Fri Jun 21 10:50:09 2013 Subject: [Histonet] Billing ??? Message-ID: Happy Friday This is a question to those who work at a hospital that have contracted pathologists. Does your hospital bill the technical component and the pathologist bill the profession component or does the pathologist bill everything and pay the hospital for technical component? Joe Maslanka BS, CT,HT (ASCP) Anatomical Pathology Technical Supervisor Give thanks for ALL things..... "Kindness is the language the blind can see & the deaf can hear- Mark Twain This electronic mail message contains information which is confidential. If you are not the intended recipient, please be aware that any disclosure, photocopying, distribution or use of the contents of the received information is prohibited. If you have received this e-mail in error, please reply to the sender immediately and permanently delete this message and all copies of it. Thank you. From mward <@t> wakehealth.edu Fri Jun 21 11:01:15 2013 From: mward <@t> wakehealth.edu (Martha Ward-Pathology) Date: Fri Jun 21 11:01:26 2013 Subject: [Histonet] Billing ??? In-Reply-To: References: Message-ID: At our institution the hospital bills the technical and the pathologist bills the professional. ? Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard ?\? Winston-Salem, NC 27157 p 336.716.2109 ?\? f 336.716.5890 ? mward@wakehealth.edu ? ? ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of JMaslanka@stpetes.org Sent: Friday, June 21, 2013 11:49 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Billing ??? Happy Friday This is a question to those who work at a hospital that have contracted pathologists. Does your hospital bill the technical component and the pathologist bill the profession component or does the pathologist bill everything and pay the hospital for technical component? Joe Maslanka BS, CT,HT (ASCP) Anatomical Pathology Technical Supervisor Give thanks for ALL things..... "Kindness is the language the blind can see & the deaf can hear- Mark Twain This electronic mail message contains information which is confidential. If you are not the intended recipient, please be aware that any disclosure, photocopying, distribution or use of the contents of the received information is prohibited. If you have received this e-mail in error, please reply to the sender immediately and permanently delete this message and all copies of it. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Joyce.Weems <@t> emoryhealthcare.org Fri Jun 21 11:07:00 2013 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Fri Jun 21 11:07:13 2013 Subject: [Histonet] Billing ??? In-Reply-To: References: Message-ID: Hospital bills technical and pathologists bill professional Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of JMaslanka@stpetes.org Sent: Friday, June 21, 2013 11:49 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Billing ??? Happy Friday This is a question to those who work at a hospital that have contracted pathologists. Does your hospital bill the technical component and the pathologist bill the profession component or does the pathologist bill everything and pay the hospital for technical component? Joe Maslanka BS, CT,HT (ASCP) Anatomical Pathology Technical Supervisor Give thanks for ALL things..... "Kindness is the language the blind can see & the deaf can hear- Mark Twain This electronic mail message contains information which is confidential. If you are not the intended recipient, please be aware that any disclosure, photocopying, distribution or use of the contents of the received information is prohibited. If you have received this e-mail in error, please reply to the sender immediately and permanently delete this message and all copies of it. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From mward <@t> wakehealth.edu Fri Jun 21 11:07:26 2013 From: mward <@t> wakehealth.edu (Martha Ward-Pathology) Date: Fri Jun 21 11:07:31 2013 Subject: [Histonet] p16 on the Bond In-Reply-To: References: Message-ID: I am having trouble posting to the histonet for some reason. I am looking for condition and incubation times for p16 on the Bond. Our new pathologist says our p16 looks overstained to him and not at all what he is accustomed to seeing and I am trying to get the staining more to his liking, without much success. Thanks in advance for the help. ? Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard ?\? Winston-Salem, NC 27157 p 336.716.2109 ?\? f 336.716.5890 ? mward@wakehealth.edu ? ? ? From TMcNemar <@t> lmhealth.org Fri Jun 21 11:48:18 2013 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Fri Jun 21 11:48:36 2013 Subject: [Histonet] Billing ??? In-Reply-To: References: Message-ID: Hospital bills the technical and pathologists bill the professional. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of JMaslanka@stpetes.org Sent: Friday, June 21, 2013 11:49 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Billing ??? Happy Friday This is a question to those who work at a hospital that have contracted pathologists. Does your hospital bill the technical component and the pathologist bill the profession component or does the pathologist bill everything and pay the hospital for technical component? Joe Maslanka BS, CT,HT (ASCP) Anatomical Pathology Technical Supervisor Give thanks for ALL things..... "Kindness is the language the blind can see & the deaf can hear- Mark Twain This electronic mail message contains information which is confidential. If you are not the intended recipient, please be aware that any disclosure, photocopying, distribution or use of the contents of the received information is prohibited. If you have received this e-mail in error, please reply to the sender immediately and permanently delete this message and all copies of it. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From Wanda.Smith <@t> HCAhealthcare.com Fri Jun 21 11:57:25 2013 From: Wanda.Smith <@t> HCAhealthcare.com (Wanda.Smith@HCAhealthcare.com) Date: Fri Jun 21 11:57:33 2013 Subject: [Histonet] Billing ??? In-Reply-To: References: Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BA27DC3116B4@NADCWPMSGCMS03.hca.corpad.net> Good Afternoon to All, At our hospital, for hospital inpatients and outpatients the Pathologist bill for their professional fee and the hospital bills the patients for the technical component. For non-patient specimens (patients seen at other offices and facilities), the Pathologist bills the patient globally for both tech and pro fee and pays the hospital for the technical services on their industrial account. Hope that helps! Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC? 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of JMaslanka@stpetes.org Sent: Friday, June 21, 2013 11:49 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Billing ??? Happy Friday This is a question to those who work at a hospital that have contracted pathologists. Does your hospital bill the technical component and the pathologist bill the profession component or does the pathologist bill everything and pay the hospital for technical component? Joe Maslanka BS, CT,HT (ASCP) Anatomical Pathology Technical Supervisor Give thanks for ALL things..... "Kindness is the language the blind can see & the deaf can hear- Mark Twain This electronic mail message contains information which is confidential. If you are not the intended recipient, please be aware that any disclosure, photocopying, distribution or use of the contents of the received information is prohibited. If you have received this e-mail in error, please reply to the sender immediately and permanently delete this message and all copies of it. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Fri Jun 21 12:27:10 2013 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Jun 21 12:27:21 2013 Subject: [Histonet] p16 on the Bond In-Reply-To: References: Message-ID: <51C454AE020000770003C333@gwmail3.harthosp.org> We dilute the CINtec p16 RTU antibody (Ventana/Roche) "1:10" (15' primary antibody incubation) and use high pH retrieval (H2) for 10' on the Bond Max. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> Martha Ward-Pathology 6/21/2013 12:07 PM >>> I am having trouble posting to the histonet for some reason. I am looking for condition and incubation times for p16 on the Bond. Our new pathologist says our p16 looks overstained to him and not at all what he is accustomed to seeing and I am trying to get the staining more to his liking, without much success. Thanks in advance for the help. Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mward@wakehealth.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Fri Jun 21 13:16:30 2013 From: pruegg <@t> ihctech.net (pruegg@ihctech.net) Date: Fri Jun 21 13:16:38 2013 Subject: [Histonet] OMICS Message-ID: <006101ce6eab$75b78ef0$6126acd0$@ihctech.net> Beware of invitations to speak at an OMICS conference, check out this link for details http://scholarlyoa.com/2013/01/25/omics-predatory-meetings/ Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E. Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 pruegghm@hotmail.com rueggihcconsultingpr@outlook.com This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. From egray <@t> hsc.wvu.edu Fri Jun 21 14:02:34 2013 From: egray <@t> hsc.wvu.edu (Gray, Ed) Date: Fri Jun 21 14:06:53 2013 Subject: [Histonet] RE: Billing ??? Message-ID: <0BBA94171603A6468FFBA03A45A029FC0443FF89AE@MSEXCL.hscresex.local> Hospital bills technical and pathologist bills technical. That's an interesting alternative though. I'm just an IT Analyst which means I know way more about billing than I want to but nowhere near enough to be a revenue cycle manager or even biller. I am interested in hearing from anyone that does both and then pays the hospital. It seems like that would be a significant benefit to everyone: The insurer/patient would only get one bill -- at least for those services; any denials would be processed together reducing work for hospital billing; and the practice plan could charge the hospital a nickel for doing it. Ed Gray | Pathology IT Analyst | Phone: 304-293-2945 | Fax 304-293-1627 | WVU Healthcare l egray@wvuhealthcare.com Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ------------------------------ Message: 12 Date: Fri, 21 Jun 2013 09:49:13 -0600 From: JMaslanka@stpetes.org Subject: [Histonet] Billing ??? To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" Happy Friday This is a question to those who work at a hospital that have contracted pathologists. Does your hospital bill the technical component and the pathologist bill the profession component or does the pathologist bill everything and pay the hospital for technical component? Joe Maslanka BS, CT,HT (ASCP) Anatomical Pathology Technical Supervisor Give thanks for ALL things..... "Kindness is the language the blind can see & the deaf can hear- Mark Twain This electronic mail message contains information which is confidential. If you are not the intended recipient, please be aware that any disclosure, photocopying, distribution or use of the contents of the received information is prohibited. If you have received this e-mail in error, please reply to the sender immediately and permanently delete this message and all copies of it. Thank you. From lpwenk <@t> sbcglobal.net Fri Jun 21 15:19:01 2013 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Fri Jun 21 15:19:10 2013 Subject: [Histonet] Fatty Fixation In-Reply-To: <2B2ECF33934F5D4996D8BE03EFDF397607D89EB2@VHAV09MSGA3.v09.med.va.gov> References: <2B2ECF33934F5D4996D8BE03EFDF397607D89EB2@VHAV09MSGA3.v09.med.va.gov> Message-ID: <5BC2382A690D4082BFA20067267A10BA@HP2010> What exactly is wrong with the fatty specimens? If the nuclei look smudgy, with no nuclear detail, then it has not been fixed long enough. If the fat is still in the tissue and you cannot section it on a microtome, then the tissue has not had enough time during processing, especially length of time in xylene and paraffin. So it would be a processing problem, not a fixation problem. And possibly a grossing problem, if the fatty tissue is grossed to thick for the length of time on the processor. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 Opinions expressed do not reflect on the hospital. -----Original Message----- From: White, Lisa M. Sent: Thursday, June 20, 2013 2:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Fatty Fixation Does anyone have a method they will share to fix fatty specimens? Does anyone utilize a stir plate? Any help greatly appreciated. We currently use Alcoholic Formalin but the results are not reliable. Lisa White HT(ASCP) Supervisory HT James H. Quillen VAMC Corner of Veterans Way and Lamont VAMC Warehouse BLDG. 205 PO Box 4000 PLMS 113 Mountain Home, TN 37684 423-979-3567 423-979-3401 fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ewj <@t> pigsqq.org Sun Jun 23 11:27:06 2013 From: ewj <@t> pigsqq.org (=?UTF-8?B?IkUuIFdheW5lIEpvaG5zb24g5pyx56iz5qOuIg==?=) Date: Sun Jun 23 11:27:17 2013 Subject: [Histonet] PCR on paraffin sections Message-ID: <51C721DA.70208@pigsqq.org> Hi. We would like to start doing PCR on paraffin fixed tissues from pigs.. It has some appeal to us because we can see the lesions in the tissue and then we can go after the causative agent post-hoc. It may be useful in cases where we have clear lesions on histopath but not the right fresh tissues for PCR. We are very limited on what we can do with IHC because of the extreme difficulty of procuring the primary Ab. It's very simple to make PCR primers here. We do have PCR up and running for all of the diseases that we are interested in. I understand that the process is basically transferring the sections to a small tube instead of a slide, then dewax with a little xylene, and remove the xylene with alcohol, then digest the section to release the viral RNA. Does anyone have any recommendations, advice, or experience with the digestion step, and can suggest an appropriate enzyme mix for this digestion? Yes I am considering in-situ hybridization but we have working PCR methods so that seems to be a simple logical step. E. Wayne Johnson Enable Ag Tech Beijing. From m.kap.1 <@t> erasmusmc.nl Sun Jun 23 12:13:51 2013 From: m.kap.1 <@t> erasmusmc.nl (M. Kap) Date: Sun Jun 23 12:13:58 2013 Subject: [Histonet] FFPE PCR In-Reply-To: <9d1e000f-45cd-4e9d-a2a5-a2fb195f0fdb@EXCH-HE03.erasmusmc.nl> References: <9d1e000f-45cd-4e9d-a2a5-a2fb195f0fdb@EXCH-HE03.erasmusmc.nl> Message-ID: ProtK digestion at 55 degrees C for half an hour will do fine There is also a new FISH like technique to detect single RNA molecules in situ, can't remember the name atm. But it works with so called Z-probes and it's very specific and sensitive. Especially when you want to look at virus in tissue it would be quite intersting to see where the virus is situated. You may even consider PAXgene fixation from now on, great histo/molecular combo fixation. Best regards, Marcel Kap Verstuurd vanaf mijn iPad Op 23 jun. 2013 om 19:05 heeft "histonet-request@lists.utsouthwestern.edu" het volgende geschreven: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. PCR on paraffin sections (E. Wayne Johnson ???) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Mon, 24 Jun 2013 00:27:06 +0800 > From: "E. Wayne Johnson ???" > Subject: [Histonet] PCR on paraffin sections > To: histonet@lists.utsouthwestern.edu > Message-ID: <51C721DA.70208@pigsqq.org> > Content-Type: text/plain; charset=UTF-8; format=flowed > > Hi. > > We would like to start doing PCR on paraffin fixed tissues from pigs.. > > It has some appeal to us because we can see the lesions in the tissue > and then we can go after the causative agent post-hoc. It may be useful > in cases where we have clear lesions on histopath but not the right > fresh tissues > for PCR. We are very limited on what we can do with IHC because > of the extreme difficulty of procuring the primary Ab. It's very > simple to make PCR primers here. > > We do have PCR up and running for all of the diseases that we > are interested in. I understand that the process is basically transferring > the sections to a small tube instead of a slide, then dewax with a little > xylene, and remove the xylene with alcohol, then digest the section > to release the viral RNA. > > Does anyone have any recommendations, advice, or experience with the > digestion > step, and can suggest an appropriate enzyme mix for this digestion? > > Yes I am considering in-situ hybridization but we have working PCR methods > so that seems to be a simple logical step. > > E. Wayne Johnson > Enable Ag Tech > Beijing. > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 115, Issue 26 > ***************************************** From sforeman <@t> labpath.com Sun Jun 23 17:06:54 2013 From: sforeman <@t> labpath.com (Susan Foreman) Date: Sun Jun 23 17:08:44 2013 Subject: [Histonet] anyone running PAX5 on smears Message-ID: <000801ce705d$f8e5eb10$eab1c130$@com> Hello, histonetters, this feed has proven helpful time after time. Does anyone run PAX5 (currently we are using DAKO antibody on Ventana stainer) on smears? We have it worked up in ffpe, but having some trouble getting staining with the smears. We have been acetone fixing the smears and the cells have stayed on beautifully, but no staining of the Bcells. I would love the help this researcher out with beautiful slides really soon J. Many Thanks, Susan Foreman, HT From Sherrian.McAnn <@t> va.gov Mon Jun 24 07:45:48 2013 From: Sherrian.McAnn <@t> va.gov (McAnn, Sherrian) Date: Mon Jun 24 07:46:12 2013 Subject: [Histonet] Fatty Fixation Message-ID: <61E2B58CECEF384094A363989D47C09009EFDF1D@VHAV17MSGA2.v17.med.va.gov> We use Dissect-Aide seems to work really well especially with lymph nodes too. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of White, Lisa M. Sent: Thursday, June 20, 2013 1:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Fatty Fixation Does anyone have a method they will share to fix fatty specimens? Does anyone utilize a stir plate? Any help greatly appreciated. We currently use Alcoholic Formalin but the results are not reliable. Lisa White HT(ASCP) Supervisory HT James H. Quillen VAMC Corner of Veterans Way and Lamont VAMC Warehouse BLDG. 205 PO Box 4000 PLMS 113 Mountain Home, TN 37684 423-979-3567 423-979-3401 fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sdysart <@t> mirnarx.com Mon Jun 24 08:24:42 2013 From: sdysart <@t> mirnarx.com (Sarah Dysart) Date: Mon Jun 24 08:24:53 2013 Subject: [Histonet] Fatty Fixation In-Reply-To: <5BC2382A690D4082BFA20067267A10BA@HP2010> References: <2B2ECF33934F5D4996D8BE03EFDF397607D89EB2@VHAV09MSGA3.v09.med.va.gov> <5BC2382A690D4082BFA20067267A10BA@HP2010> Message-ID: <32bd7d6805b54bf0ae507fe7c0c82181@BLUPR07MB001.namprd07.prod.outlook.com> Try formalin-aceto-alcohol (F-A-A)...it works pretty well =) Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk Sent: Friday, June 21, 2013 3:19 PM To: White, Lisa M.; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Fatty Fixation What exactly is wrong with the fatty specimens? If the nuclei look smudgy, with no nuclear detail, then it has not been fixed long enough. If the fat is still in the tissue and you cannot section it on a microtome, then the tissue has not had enough time during processing, especially length of time in xylene and paraffin. So it would be a processing problem, not a fixation problem. And possibly a grossing problem, if the fatty tissue is grossed to thick for the length of time on the processor. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 Opinions expressed do not reflect on the hospital. -----Original Message----- From: White, Lisa M. Sent: Thursday, June 20, 2013 2:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Fatty Fixation Does anyone have a method they will share to fix fatty specimens? Does anyone utilize a stir plate? Any help greatly appreciated. We currently use Alcoholic Formalin but the results are not reliable. Lisa White HT(ASCP) Supervisory HT James H. Quillen VAMC Corner of Veterans Way and Lamont VAMC Warehouse BLDG. 205 PO Box 4000 PLMS 113 Mountain Home, TN 37684 423-979-3567 423-979-3401 fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sdysart <@t> mirnarx.com Mon Jun 24 08:26:06 2013 From: sdysart <@t> mirnarx.com (Sarah Dysart) Date: Mon Jun 24 08:26:25 2013 Subject: [Histonet] RE: FFPE PCR In-Reply-To: References: <9d1e000f-45cd-4e9d-a2a5-a2fb195f0fdb@EXCH-HE03.erasmusmc.nl> Message-ID: <8d916070d01645a2ab3e8b05f4610cfc@BLUPR07MB001.namprd07.prod.outlook.com> I do the PK digestion at 55C overnight and get the best results Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of M. Kap Sent: Sunday, June 23, 2013 12:14 PM To: Subject: [Histonet] FFPE PCR ProtK digestion at 55 degrees C for half an hour will do fine There is also a new FISH like technique to detect single RNA molecules in situ, can't remember the name atm. But it works with so called Z-probes and it's very specific and sensitive. Especially when you want to look at virus in tissue it would be quite intersting to see where the virus is situated. You may even consider PAXgene fixation from now on, great histo/molecular combo fixation. Best regards, Marcel Kap Verstuurd vanaf mijn iPad Op 23 jun. 2013 om 19:05 heeft "histonet-request@lists.utsouthwestern.edu" het volgende geschreven: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. PCR on paraffin sections (E. Wayne Johnson ???) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Mon, 24 Jun 2013 00:27:06 +0800 > From: "E. Wayne Johnson ???" > Subject: [Histonet] PCR on paraffin sections > To: histonet@lists.utsouthwestern.edu > Message-ID: <51C721DA.70208@pigsqq.org> > Content-Type: text/plain; charset=UTF-8; format=flowed > > Hi. > > We would like to start doing PCR on paraffin fixed tissues from pigs.. > > It has some appeal to us because we can see the lesions in the tissue > and then we can go after the causative agent post-hoc. It may be useful > in cases where we have clear lesions on histopath but not the right > fresh tissues > for PCR. We are very limited on what we can do with IHC because > of the extreme difficulty of procuring the primary Ab. It's very > simple to make PCR primers here. > > We do have PCR up and running for all of the diseases that we > are interested in. I understand that the process is basically transferring > the sections to a small tube instead of a slide, then dewax with a little > xylene, and remove the xylene with alcohol, then digest the section > to release the viral RNA. > > Does anyone have any recommendations, advice, or experience with the > digestion > step, and can suggest an appropriate enzyme mix for this digestion? > > Yes I am considering in-situ hybridization but we have working PCR methods > so that seems to be a simple logical step. > > E. Wayne Johnson > Enable Ag Tech > Beijing. > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 115, Issue 26 > ***************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpjones <@t> srhs-pa.org Mon Jun 24 10:21:43 2013 From: lpjones <@t> srhs-pa.org (Jones, Laura) Date: Mon Jun 24 10:21:23 2013 Subject: [Histonet] Screen Cassettes Message-ID: <4AE8039AEA096143B965CBC6D092166802351B3A4F@EXCH2007.srhs-pa.org> Good morning and Happy Monday to all. We have 18 boxes of Thermo brand screen cassettes, product #0071120-00-22D and light green in color, that our Pathologist has decided he no longer wants. If anyone is interested in these, please contact Mary Ellen Maravich at mmaravich@srhs-pa.org There is nothing wrong with them that we are aware of, and the boxes have never been opened. Thanks in advance! ________________________________ Sharon Regional Health System is the area's largest hospital and provider of health care services. Visit us online at http://www.sharonregional.com for a complete listing of our services, primary care physicians and specialists, and satellite locations. Confidentiality Note: This message is intended for use only by the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. Thank you. From foreightl <@t> gmail.com Mon Jun 24 10:59:38 2013 From: foreightl <@t> gmail.com (Patrick Laurie) Date: Mon Jun 24 10:59:42 2013 Subject: [Histonet] Block labeling Message-ID: Hi Everyone, What is the best way to label blocks that are already embedded? We have a large control bank that we are trying to relabel and make search able, however, most marking pens don't write well on paraffin covered blocks, even when we scrape them off. Pencils work, not always the most legibly, so I was wondering if there is a clever solution that someone might have found. We've tried putting stickers or labels on the back, but that falls right off. Thanks in advance for any suggestions. -- Patrick Laurie(HT)ASCP QIHC Histology Manager Celligent Diagnostics, LLC 101 East W.T. Harris Blvd | Suite 1212 | Charlotte, NC 28262 Work: 704-970-3300 Cell: 704-266-0869 From lblazek <@t> digestivespecialists.com Mon Jun 24 11:19:08 2013 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Mon Jun 24 11:23:07 2013 Subject: [Histonet] Block labeling In-Reply-To: References: Message-ID: <5A2BD13465E061429D6455C8D6B40E391656A9E086@IBMB7Exchange.digestivespecialists.com> Put a label on the back of the block and then a couple of drops of paraffin. It acts like glue! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patrick Laurie Sent: Monday, June 24, 2013 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Block labeling Hi Everyone, What is the best way to label blocks that are already embedded? We have a large control bank that we are trying to relabel and make search able, however, most marking pens don't write well on paraffin covered blocks, even when we scrape them off. Pencils work, not always the most legibly, so I was wondering if there is a clever solution that someone might have found. We've tried putting stickers or labels on the back, but that falls right off. Thanks in advance for any suggestions. -- Patrick Laurie(HT)ASCP QIHC Histology Manager Celligent Diagnostics, LLC 101 East W.T. Harris Blvd | Suite 1212 | Charlotte, NC 28262 Work: 704-970-3300 Cell: 704-266-0869 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 <@t> earthlink.net Mon Jun 24 11:37:33 2013 From: relia1 <@t> earthlink.net (Pam Barker) Date: Mon Jun 24 11:37:38 2013 Subject: [Histonet] RELIA HOT Job Alert - Lead Histotech for Brand New Dermpath Lab in Long Beach, CA Have Frozen Sections Experience? Learn Mohs!!! Message-ID: <013c01ce70f9$20a8bce0$61fa36a0$@earthlink.net> Hi Histonetters!! I hope your week is off to a great start. I have an exciting new opportunity to tell you about. I am working with a Dermatopathology practice in Long Beach, CA that wants to hire an ASCP certified histotech with frozen sections experience and will train in Mohs. This is a permanent full time day shift position. You would be the sole practitioner histo tech in their brand new state of the art lab. For more information please give me a call or shoot me an email. Thanks-Pam You can reach me toll free at 866-607-3542 until 5p EST or anytime on my cell phone at 407-353-5070 or via email at relia1@earthlink.net Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From TMcNemar <@t> lmhealth.org Mon Jun 24 11:51:53 2013 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Mon Jun 24 11:51:54 2013 Subject: [Histonet] Block labeling In-Reply-To: References: Message-ID: I used to use a 4x4 with a little Americlear or Xylene to clean the paraffin off before relabeling. You can then dip or run a little paraffin over it. May not be practical if you have a lot. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patrick Laurie Sent: Monday, June 24, 2013 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Block labeling Hi Everyone, What is the best way to label blocks that are already embedded? We have a large control bank that we are trying to relabel and make search able, however, most marking pens don't write well on paraffin covered blocks, even when we scrape them off. Pencils work, not always the most legibly, so I was wondering if there is a clever solution that someone might have found. We've tried putting stickers or labels on the back, but that falls right off. Thanks in advance for any suggestions. -- Patrick Laurie(HT)ASCP QIHC Histology Manager Celligent Diagnostics, LLC 101 East W.T. Harris Blvd | Suite 1212 | Charlotte, NC 28262 Work: 704-970-3300 Cell: 704-266-0869 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From jaylundgren <@t> gmail.com Mon Jun 24 13:04:15 2013 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Mon Jun 24 13:04:21 2013 Subject: [Histonet] Block labeling In-Reply-To: References: Message-ID: Scrape with a knife, AND clean block with gauze and xylene (under a hood), relabel with permanent marker, dip re-labeled block in paraffin to protect. The trick is to get ALL the paraffin off the writing surface. On Mon, Jun 24, 2013 at 11:51 AM, Tom McNemar wrote: > I used to use a 4x4 with a little Americlear or Xylene to clean the > paraffin off before relabeling. You can then dip or run a little paraffin > over it. May not be practical if you have a lot. > > Tom McNemar, HT(ASCP) > Histology Co-ordinator > Licking Memorial Health Systems > (740) 348-4163 > (740) 348-4166 > tmcnemar@lmhealth.org > www.LMHealth.org > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patrick Laurie > Sent: Monday, June 24, 2013 12:00 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Block labeling > > Hi Everyone, > > What is the best way to label blocks that are already embedded? We have a > large control bank that we are trying to relabel and make search able, > however, most marking pens don't write well on paraffin covered blocks, > even when we scrape them off. Pencils work, not always the most legibly, > so I was wondering if there is a clever solution that someone might have > found. We've tried putting stickers or labels on the back, but that falls > right off. > > Thanks in advance for any suggestions. > > -- > > Patrick Laurie(HT)ASCP QIHC > > Histology Manager > > Celligent Diagnostics, LLC > > 101 East W.T. Harris Blvd | Suite 1212 | Charlotte, NC 28262 > > Work: 704-970-3300 Cell: 704-266-0869 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This e-mail, including attachments, is intended for the sole use of the > individual and/or entity to whom it is addressed, and contains information > from Licking Memorial Health Systems which is confidential or privileged. > If you are not the intended recipient, nor authorized to receive for the > intended recipient, be aware that any disclosure, copying, distribution or > use of the contents of this e-mail and attachments is prohibited. If you > have received this in error, please advise the sender by reply e-mail and > delete the message immediately. You may also contact the LMH Process > Improvement Center at 740-348-4641. E-mail transmissions cannot be > guaranteed to be secure or error-free as information could be intercepted, > corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. > The sender therefore does not accept liability for any errors or omissions > in the contents of this message, which arise as a result of e-mail > transmission. Thank you. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From hfedor <@t> jhmi.edu Mon Jun 24 13:07:58 2013 From: hfedor <@t> jhmi.edu (Helen Fedor) Date: Mon Jun 24 13:08:04 2013 Subject: [Histonet] Block labeling In-Reply-To: References: Message-ID: Hello, we are using the labels from the "Brady" label maker with good success. They are extra sticky and you can find some that will stick to paraffin. We use ones that qualify for freezing since they need to be safe on the ice tray. http://www.bradycorp.com/ Helen -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patrick Laurie Sent: Monday, June 24, 2013 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Block labeling Hi Everyone, What is the best way to label blocks that are already embedded? We have a large control bank that we are trying to relabel and make search able, however, most marking pens don't write well on paraffin covered blocks, even when we scrape them off. Pencils work, not always the most legibly, so I was wondering if there is a clever solution that someone might have found. We've tried putting stickers or labels on the back, but that falls right off. Thanks in advance for any suggestions. -- Patrick Laurie(HT)ASCP QIHC Histology Manager Celligent Diagnostics, LLC 101 East W.T. Harris Blvd | Suite 1212 | Charlotte, NC 28262 Work: 704-970-3300 Cell: 704-266-0869 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jaylundgren <@t> gmail.com Mon Jun 24 13:46:03 2013 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Mon Jun 24 13:46:10 2013 Subject: [Histonet] Block labeling In-Reply-To: References: Message-ID: IMHO, any stick on label, no matter how good the adhesive, is not as safe as writing on the block itself. On Mon, Jun 24, 2013 at 1:07 PM, Helen Fedor wrote: > Hello, we are using the labels from the "Brady" label maker with good > success. They are extra sticky and you can find some that will stick to > paraffin. We use ones that qualify for freezing since they need to be safe > on the ice tray. > > http://www.bradycorp.com/ > > Helen > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patrick Laurie > Sent: Monday, June 24, 2013 12:00 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Block labeling > > Hi Everyone, > > What is the best way to label blocks that are already embedded? We have a > large control bank that we are trying to relabel and make search able, > however, most marking pens don't write well on paraffin covered blocks, > even when we scrape them off. Pencils work, not always the most legibly, > so I was wondering if there is a clever solution that someone might have > found. We've tried putting stickers or labels on the back, but that falls > right off. > > Thanks in advance for any suggestions. > > -- > > Patrick Laurie(HT)ASCP QIHC > > Histology Manager > > Celligent Diagnostics, LLC > > 101 East W.T. Harris Blvd | Suite 1212 | Charlotte, NC 28262 > > Work: 704-970-3300 Cell: 704-266-0869 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From LSebree <@t> uwhealth.org Mon Jun 24 13:56:42 2013 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Mon Jun 24 13:56:48 2013 Subject: [Histonet] IHC antibody for Major Basic Protein for eosinophils Message-ID: <77DD817201982748BC67D7960F2F76AF04FA6D@UWHC-MBX12.uwhis.hosp.wisc.edu> Are there any reference labs offering this on FFPE specimens? Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 From JMacDonald <@t> mtsac.edu Mon Jun 24 16:26:03 2013 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Mon Jun 24 16:26:10 2013 Subject: [Histonet] Need Current Histology Benchmark Data Message-ID: Does anyone have current benchmark data? ? like expected average # blocks/slides, per day ? From joelleweaver <@t> hotmail.com Mon Jun 24 17:36:40 2013 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Mon Jun 24 17:36:46 2013 Subject: [Histonet] Need Current Histology Benchmark Data In-Reply-To: References: Message-ID: There is this pretty recent article http://www.archivesofpathology.org/doi/pdf/10.1043/2010-0288-CP.1 Joelle Weaver MAOM, HTL (ASCP) QIHC To: histonet@lists.utsouthwestern.edu From: JMacDonald@mtsac.edu Date: Mon, 24 Jun 2013 14:26:03 -0700 Subject: [Histonet] Need Current Histology Benchmark Data Does anyone have current benchmark data? ? like expected average # blocks/slides, per day ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From christen <@t> vet.k-state.edu Tue Jun 25 06:46:44 2013 From: christen <@t> vet.k-state.edu (Shelly Christenson) Date: Tue Jun 25 06:46:59 2013 Subject: [Histonet] Histology Lab Position opening Message-ID: <06E342B6098ED9478347E1407764C80407D5CDB9@VETMXHT.ads.vet.k-state.edu> We have an opening in our histology lab at the Veterinary Diagnostic Laboratory at Kansas State University in Manhattan Kansas. Information on the position is listed below. If you want more information about the area or the job you can contact me or Brooke Stallbaumer. Thanks for your interest, Shelly Veterinary Diagnostic Laboratory Research Assistant in Histopathology Lab A term Research Assistant position in the Veterinary Diagnostic Laboratory at Kansas State University is available. A Bachelor of Science and one year of laboratory experience is required. An individual with a Certification in Histotechnology or histology experience is desirable. A background in laboratory methods and procedures is beneficial. Maintaining harmonious working relationships with co-workers and other employees is a must. A positive customer service attitude is essential. The individual must be able to work cooperatively in scheduling work hours with co-workers such that hours outside of 8-5 may be expected, and work could include Saturdays/weekend duties. Application screening begins July 8, 2013 and continues until the position is filled. Submit a cover letter, resume, and contact information for 3 Professional references to Brooke Stallbaumer, Kansas State University, 102 Trotter Hall, Manhattan, KS 66506 or email bstall@vet.ksu.edu. KSU is an AA/EOE. Background check required. Shelly Christenson HT (ASCP) Veterinary Diagnostic Lab Histopathology Mosier Hall L-216 Kansas State University 1800 Denison Manhattan, KS 66506 785/532-4464 christen@vet.k-state.edu From Caroline.Pratt <@t> uphs.upenn.edu Tue Jun 25 10:11:29 2013 From: Caroline.Pratt <@t> uphs.upenn.edu (Pratt, Caroline) Date: Tue Jun 25 10:12:01 2013 Subject: [Histonet] Per Diem Techs Message-ID: The Dermatopathology lab at Penn Medicine is looking for per diem HT's that can work immediately any shift but preferably nights. We operate 24/5. If you are interested, please email me your resume at Caroline.Pratt@uphs.upenn.edu. Thanks! Caroline M. Pratt, MBA Practice Administrator Dermpath 3020 Market Street, Ste 201 Philadelphia, PA 19104 Phone 215-349-8178 Fax 215-662-6150 The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From SJMccabe <@t> drmc.org Tue Jun 25 11:22:36 2013 From: SJMccabe <@t> drmc.org (McCabe, Sara) Date: Tue Jun 25 11:25:32 2013 Subject: [Histonet] RE: p16 on Bond In-Reply-To: <844c7cc2-6cdd-4cb3-880d-3bcb892cd6c9@EX07.drmc.org> References: <844c7cc2-6cdd-4cb3-880d-3bcb892cd6c9@EX07.drmc.org> Message-ID: <02AE2390303AAB43A823930EAD6B63AE668A86B5@ex07> Richard, We currently do not dilute the p16 antibody. We run it on the Bond using ER1 for 20 minutes. Sara McCabe, HT (ASCP) DuBois Regional Medical Center This email and any attached files are confidential and intended solely for the intended recipient(s). If you are not the named recipient you should not read, distribute, copy or alter this email. Any views or opinions expressed in this email are those of the author and do not represent those of the company. Warning: Although precautions have been taken to make sure no viruses are present in this email, the company cannot accept responsibility for any loss or damage that arise from the use of this email or attachments. From joelleweaver <@t> hotmail.com Tue Jun 25 11:29:38 2013 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Tue Jun 25 11:39:23 2013 Subject: [Histonet] Need Current Histology Benchmark Data In-Reply-To: References: Message-ID: PGXL Laboratories, located in Louisville, KY, is seeking a HT (ASCP) or HTL (ASCP) for its newly created histology laboratory. The histology lab section will support the existing molecular and pharmacogenommics services provided by this molecular laboratory. This is a unique opportunity for a highly motivated individual, which will provide an oppportunity for much more variety and challenge than the typical histology position. Seeking: ASCP histotechnician/histotechnologist certification.Experience in histochemical staining ( all aspects), immunohistochemistry, in- situ hybridization, and FISH is preferred. Education and training meeting CLIA high complexity requirements highly desired. Interested candidates should send their resume to HR@pgxlab.com. You will get an automated response, however, I can also provide more details and answer many of the questions you may have about the position. Please contact me at this email for more information. Thank you! Joelle Weaver MAOM, HTL (ASCP) QIHC To: histonet@lists.utsouthwestern.edu From: JMacDonald@mtsac.edu Date: Mon, 24 Jun 2013 14:26:03 -0700 Subject: [Histonet] Need Current Histology Benchmark Data Does anyone have current benchmark data? ? like expected average # blocks/slides, per day ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rheyna <@t> lumc.edu Tue Jun 25 11:53:43 2013 From: rheyna <@t> lumc.edu (Roger Heyna) Date: Tue Jun 25 11:53:49 2013 Subject: [Histonet] Adenovirus Tissue Controls Needed Message-ID: <51C984C7020000230007C356@gwgwia1.luhs.org> We have been using tissues from an old autopsy case as our adenovirus IHC controls, and we are nearly out. I am not able to find another adenovirus case within our institution that we can use for controls. Does anyone know a vendor that supplies these controls? Also, is there a reference lab that performs this test via IHC? Our usual three reference labs do not offer this stain. And lastly, if anyone has extra adenovirus-infected tissues, would you be willing to send us some? We would be willing to pay for it. Thank you in advance for your help. Roger Maywood, IL From Lisa.White3 <@t> va.gov Tue Jun 25 12:19:06 2013 From: Lisa.White3 <@t> va.gov (White, Lisa M.) Date: Tue Jun 25 12:19:28 2013 Subject: : [Histonet] Block labeling Message-ID: <2B2ECF33934F5D4996D8BE03EFDF397607D89ECB@VHAV09MSGA3.v09.med.va.gov> Before you put the label on the back touch the back of the block to the hot plate on your embedding unit for a few seconds, the label will stick when the wax is warm. If you have a really stubborn one pour a little wax over the label to seal it into the back of the block. You will still be able to read the label if it is in dark ink. Lisa White HT(ASCP) Supervisory HT James H. Quillen VAMC Corner of Veterans Way and Lamont VAMC Warehouse BLDG. 205 PO Box 4000 PLMS 113 Mountain Home, TN 37684 423-979-3567 423-979-3401 fax From Rcartun <@t> harthosp.org Tue Jun 25 12:31:40 2013 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Tue Jun 25 12:31:48 2013 Subject: [Histonet] Adenovirus Tissue Controls Needed In-Reply-To: <51C984C7020000230007C356@gwgwia1.luhs.org> References: <51C984C7020000230007C356@gwgwia1.luhs.org> Message-ID: <51C99BBC020000770003C547@gwmail3.harthosp.org> I do IHC for Adenovirus in my laboratory. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> Roger Heyna 6/25/2013 12:53 PM >>> We have been using tissues from an old autopsy case as our adenovirus IHC controls, and we are nearly out. I am not able to find another adenovirus case within our institution that we can use for controls. Does anyone know a vendor that supplies these controls? Also, is there a reference lab that performs this test via IHC? Our usual three reference labs do not offer this stain. And lastly, if anyone has extra adenovirus-infected tissues, would you be willing to send us some? We would be willing to pay for it. Thank you in advance for your help. Roger Maywood, IL From tfraser <@t> olympicmedical.org Tue Jun 25 12:42:13 2013 From: tfraser <@t> olympicmedical.org (Tasha Fraser) Date: Tue Jun 25 12:42:24 2013 Subject: [Histonet] Epic and PowerPath Message-ID: <5290DE49B23B6240BC0DF14FC8142D1901CB9106@is-210vs.olympicmedical.local> Does anyone out there use Epic and PowerPath together? Tasha R. Fraser, HT (ASCP) Olympic Medical Center 939 Caroline Street Port Angeles, WA 98362 ------------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended individual(s) named above and may contain confidential, privileged, and/or protected information. Any unauthorized review, use, disclosure, copying, or distribution of its contents is prohibited. If you are not the intended recipient, you have received this email in error. If so, please notify the sender immediately by reply email and delete/destroy the original and all copies of this communication. Also know that Internet e-mail is not secure. In choosing to communicate with Olympic Medical Center by email you will assume these confidentiality risks. Internet messages may become corrupted, incomplete, or may incorrectly identify the sender. From JEllin <@t> yumaregional.org Tue Jun 25 12:53:08 2013 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Tue Jun 25 12:53:19 2013 Subject: [Histonet] RE: Epic and PowerPath In-Reply-To: <5290DE49B23B6240BC0DF14FC8142D1901CB9106@is-210vs.olympicmedical.local> References: <5290DE49B23B6240BC0DF14FC8142D1901CB9106@is-210vs.olympicmedical.local> Message-ID: We use EPIC and PowerPath,, how can I help you... Jesus Ellin Yuma Regional Medical Center 928-336-1743 jellin@yumaregional.org ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Tasha Fraser [tfraser@olympicmedical.org] Sent: Tuesday, June 25, 2013 10:42 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Epic and PowerPath Does anyone out there use Epic and PowerPath together? Tasha R. Fraser, HT (ASCP) Olympic Medical Center 939 Caroline Street Port Angeles, WA 98362 ------------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended individual(s) named above and may contain confidential, privileged, and/or protected information. Any unauthorized review, use, disclosure, copying, or distribution of its contents is prohibited. If you are not the intended recipient, you have received this email in error. If so, please notify the sender immediately by reply email and delete/destroy the original and all copies of this communication. Also know that Internet e-mail is not secure. In choosing to communicate with Olympic Medical Center by email you will assume these confidentiality risks. Internet messages may become corrupted, incomplete, or may incorrectly identify the sender. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ From plott <@t> uab.edu Tue Jun 25 13:10:30 2013 From: plott <@t> uab.edu (Patricia F Lott) Date: Tue Jun 25 13:10:35 2013 Subject: [Histonet] need clamp Message-ID: <372C4AF089B6AE48B36F064FA891FF78137D1B69@UABEXMB3.ad.uab.edu> Does anyone have an extra lateral displacement clamp lever for Leica 2135 or 2265? From sforeman <@t> labpath.com Tue Jun 25 13:10:59 2013 From: sforeman <@t> labpath.com (Susan Foreman) Date: Tue Jun 25 13:12:55 2013 Subject: [Histonet] HPV ISH Probes Message-ID: <003901ce71cf$58982ce0$09c886a0$@com> Does anyone have HPV-ISH working? utilizing a vendor for the probe other than Ventana? Prep Kit? Many Thanks, Susan Foreman, HT (ASCP) (865)584-1933 From joelleweaver <@t> hotmail.com Tue Jun 25 13:36:58 2013 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Tue Jun 25 13:37:02 2013 Subject: [Histonet] HPV ISH Probes In-Reply-To: <003901ce71cf$58982ce0$09c886a0$@com> References: <003901ce71cf$58982ce0$09c886a0$@com> Message-ID: Give me about a month. Enzo Joelle Weaver MAOM, HTL (ASCP) QIHC > From: sforeman@labpath.com > To: histonet@lists.utsouthwestern.edu > Date: Tue, 25 Jun 2013 14:10:59 -0400 > Subject: [Histonet] HPV ISH Probes > > Does anyone have HPV-ISH working? utilizing a vendor for the probe other > than Ventana? Prep Kit? > > > > > > Many Thanks, > > Susan Foreman, HT (ASCP) > (865)584-1933 > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Tue Jun 25 14:33:55 2013 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Tue Jun 25 14:33:59 2013 Subject: [Histonet] HPV ISH Probes In-Reply-To: <003901ce71cf$58982ce0$09c886a0$@com> References: <003901ce71cf$58982ce0$09c886a0$@com> Message-ID: HPV6, 11, 16 18, 31, 33 & 51 "screening' Joelle Weaver MAOM, HTL (ASCP) QIHC > From: sforeman@labpath.com > To: histonet@lists.utsouthwestern.edu > Date: Tue, 25 Jun 2013 14:10:59 -0400 > Subject: [Histonet] HPV ISH Probes > > Does anyone have HPV-ISH working? utilizing a vendor for the probe other > than Ventana? Prep Kit? > > > > > > Many Thanks, > > Susan Foreman, HT (ASCP) > (865)584-1933 > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Ronald.Houston <@t> nationwidechildrens.org Tue Jun 25 14:41:28 2013 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Tue Jun 25 14:41:45 2013 Subject: [Histonet] HPV ISH Probes In-Reply-To: References: <003901ce71cf$58982ce0$09c886a0$@com> Message-ID: Many people are working on the Enzo probes just now. Mayo does have the probes up and running on the Bonds, but are keeping tight-lipped about their protocols. We tried the Dako probes but never could get consistent results. Ronnie Houston MS HT(ASCP)QIHC -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelle weaver Sent: Tuesday, June 25, 2013 3:34 PM To: Susan Foreman; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HPV ISH Probes HPV6, 11, 16 18, 31, 33 & 51 "screening' Joelle Weaver MAOM, HTL (ASCP) QIHC > From: sforeman@labpath.com > To: histonet@lists.utsouthwestern.edu > Date: Tue, 25 Jun 2013 14:10:59 -0400 > Subject: [Histonet] HPV ISH Probes > > Does anyone have HPV-ISH working? utilizing a vendor for the probe > other than Ventana? Prep Kit? > > > > > > Many Thanks, > > Susan Foreman, HT (ASCP) > (865)584-1933 > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lizronan <@t> umich.edu Tue Jun 25 14:48:26 2013 From: lizronan <@t> umich.edu (Elizabeth Ronan) Date: Tue Jun 25 14:48:33 2013 Subject: [Histonet] Dyeing engineered tissues prior to implantation for easier identification Message-ID: Hi everyone, My lab implants tissue engineered constructs into animal models and we are having trouble distinguishing what is actually implanted tissue at the time of explant. Is there a way to dye or mark our tissue grafts prior to implantation? We plan on doing IHC on the explants so we don't want any auto fluorescence caused by the dye to interfere. We've thrown around the idea of staining the construct with evan's blue, however it is excited in the red channel. I was wondering if anyone out there had any ideas for a tracer dye we could stain our constructs with that will allow it to be visibly identifiable once implanted into animals and that would not interfere with IHC or elicit an immune response. Thanks, Liz From joelleweaver <@t> hotmail.com Tue Jun 25 15:09:12 2013 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Tue Jun 25 15:09:21 2013 Subject: [Histonet] HPV ISH Probes In-Reply-To: References: <003901ce71cf$58982ce0$09c886a0$@com>, , Message-ID: Also using Bond, will let you know. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: Ronald.Houston@nationwidechildrens.org > To: joelleweaver@hotmail.com; sforeman@labpath.com; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] HPV ISH Probes > Date: Tue, 25 Jun 2013 19:41:28 +0000 > > Many people are working on the Enzo probes just now. Mayo does have the probes up and running on the Bonds, but are keeping tight-lipped about their protocols. > We tried the Dako probes but never could get consistent results. > > Ronnie Houston MS HT(ASCP)QIHC > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelle weaver > Sent: Tuesday, June 25, 2013 3:34 PM > To: Susan Foreman; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] HPV ISH Probes > > HPV6, 11, 16 18, 31, 33 & 51 "screening' > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > From: sforeman@labpath.com > > To: histonet@lists.utsouthwestern.edu > > Date: Tue, 25 Jun 2013 14:10:59 -0400 > > Subject: [Histonet] HPV ISH Probes > > > > Does anyone have HPV-ISH working? utilizing a vendor for the probe > > other than Ventana? Prep Kit? > > > > > > > > > > > > Many Thanks, > > > > Susan Foreman, HT (ASCP) > > (865)584-1933 > > > > > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CObregon <@t> mhs.net Tue Jun 25 15:32:42 2013 From: CObregon <@t> mhs.net (Obregon, Cecilia) Date: Tue Jun 25 15:33:05 2013 Subject: [Histonet] HPV ISH Probes In-Reply-To: References: <003901ce71cf$58982ce0$09c886a0$@com> , Message-ID: <598BB4A3A92D7F4DAA14C30E4AE70C2F10B1B95C@MHSEXMB05.mhs.net> I have personally tried the Dako probes on the Ventana platform but no luck so far. I have heard about the Enzo probes been succesfully worked up at other institutions. Does anybody know if a 'link antibody' is needed for the Enzo probes? Greatly appreciate any inputs. Cecilia M. Obregon, HTL (ASCP) Memorial Regional Hospital 3501 Johnson Street Hollywood, FL 33021 Ph: 954-265-5317 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Houston, Ronald [Ronald.Houston@nationwidechildrens.org] Sent: Tuesday, June 25, 2013 3:41 PM To: joelle weaver; Susan Foreman; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HPV ISH Probes Many people are working on the Enzo probes just now. Mayo does have the probes up and running on the Bonds, but are keeping tight-lipped about their protocols. We tried the Dako probes but never could get consistent results. Ronnie Houston MS HT(ASCP)QIHC -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelle weaver Sent: Tuesday, June 25, 2013 3:34 PM To: Susan Foreman; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HPV ISH Probes HPV6, 11, 16 18, 31, 33 & 51 "screening' Joelle Weaver MAOM, HTL (ASCP) QIHC > From: sforeman@labpath.com > To: histonet@lists.utsouthwestern.edu > Date: Tue, 25 Jun 2013 14:10:59 -0400 > Subject: [Histonet] HPV ISH Probes > > Does anyone have HPV-ISH working? utilizing a vendor for the probe > other than Ventana? Prep Kit? > > > > > > Many Thanks, > > Susan Foreman, HT (ASCP) > (865)584-1933 > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vikk_0521 <@t> yahoo.com Tue Jun 25 20:45:10 2013 From: vikk_0521 <@t> yahoo.com (Vikrant Piprode) Date: Tue Jun 25 20:45:15 2013 Subject: [Histonet] Thought you'd find this interesting Message-ID: <0.0.0.6A3.1CE720ECB1C5FE0.0@vmta06.monkeyinferno.com> Hi! I?ve just learned about the water crisis and thought you would be interested to check out this story: https://waterforward.charitywater.org/et/jfPeUNkk Let me know what you think! Thanks, Vikrant -- Sent via WaterForward, an initiative of charity: water WaterForward, 387 Tehama Street, San Francisco, CA 94103, USA. Click here to unsubscribe: https://waterforward.charitywater.org/opt_out?et=jfPeUNkk From akbitting <@t> geisinger.edu Wed Jun 26 08:27:55 2013 From: akbitting <@t> geisinger.edu (Bitting, Angela K.) Date: Wed Jun 26 08:28:03 2013 Subject: [Histonet] HPV ISH Probes In-Reply-To: <598BB4A3A92D7F4DAA14C30E4AE70C2F10B1B95C@MHSEXMB05.mhs.net> References: <003901ce71cf$58982ce0$09c886a0$@com> , <598BB4A3A92D7F4DAA14C30E4AE70C2F10B1B95C@MHSEXMB05.mhs.net> Message-ID: <77F52EFAB8B1694B885E277C48FCD0F64A70EF15@GHSEXMBX4W8K1V.geisinger.edu> I believe so, yes. This is the info I was given. Buy Biotin Antibody Rabbit Polyclonal: A150-109A from Bethyl Laboratories, Inc. 25043 West FM 1097, Montgomery, TX 77356, 1-800-338-9579 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Obregon, Cecilia Sent: Tuesday, June 25, 2013 4:33 PM To: Houston, Ronald; joelle weaver; Susan Foreman; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HPV ISH Probes I have personally tried the Dako probes on the Ventana platform but no luck so far. I have heard about the Enzo probes been succesfully worked up at other institutions. Does anybody know if a 'link antibody' is needed for the Enzo probes? Greatly appreciate any inputs. Cecilia M. Obregon, HTL (ASCP) Memorial Regional Hospital 3501 Johnson Street Hollywood, FL 33021 Ph: 954-265-5317 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Houston, Ronald [Ronald.Houston@nationwidechildrens.org] Sent: Tuesday, June 25, 2013 3:41 PM To: joelle weaver; Susan Foreman; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HPV ISH Probes Many people are working on the Enzo probes just now. Mayo does have the probes up and running on the Bonds, but are keeping tight-lipped about their protocols. We tried the Dako probes but never could get consistent results. Ronnie Houston MS HT(ASCP)QIHC -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelle weaver Sent: Tuesday, June 25, 2013 3:34 PM To: Susan Foreman; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HPV ISH Probes HPV6, 11, 16 18, 31, 33 & 51 "screening' Joelle Weaver MAOM, HTL (ASCP) QIHC > From: sforeman@labpath.com > To: histonet@lists.utsouthwestern.edu > Date: Tue, 25 Jun 2013 14:10:59 -0400 > Subject: [Histonet] HPV ISH Probes > > Does anyone have HPV-ISH working? utilizing a vendor for the probe > other than Ventana? Prep Kit? > > > > > > Many Thanks, > > Susan Foreman, HT (ASCP) > (865)584-1933 > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. ------------------------------------------------------------------------- This message was secured by ZixCorp(R). From m_chadwell <@t> hotmail.com Wed Jun 26 09:49:33 2013 From: m_chadwell <@t> hotmail.com (margaret chadwell) Date: Wed Jun 26 09:52:37 2013 Subject: =?utf-8?Q?Re:_[Histonet]_HPV_ISH_Probes?= Message-ID: We have gotten good results with the following probes. http://www.panomics.com/products/rna-in-situ-analysis/viewrna-ish-tissue-assay/overview At first they were not working well but the rep come out for the day and went over them with us and not the results are beautiful and consistent. Margie Chadwell Sent from Windows Mail From: Bitting, Angela K. Sent: ?Wednesday?, ?June? ?26?, ?2013 ?6?:?28? ?AM To: Obregon, Cecilia, Houston, Ronald, joelle weaver, Susan Foreman, histonet@lists.utsouthwestern.edu I believe so, yes. This is the info I was given. Buy Biotin Antibody Rabbit Polyclonal: A150-109A from Bethyl Laboratories, Inc. 25043 West FM 1097, Montgomery, TX 77356, 1-800-338-9579 -----Original Message--http://www.panomics.com/products/rna-in-situ-analysis/viewrna-ish-tissue-assay/overview--- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Obregon, Cecilia Sent: Tuesday, June 25, 2013 4:33 PM To: Houston, Ronald; joelle weaver; Susan Foreman; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HPV ISH Probes I have personally tried the Dako probes on the Ventana platform but no luck so far. I have heard about the Enzo probes been succesfully worked up at other institutions. Does anybody know if a 'link antibody' is needed for the Enzo probes? Greatly appreciate any inputs. Cecilia M. Obregon, HTL (ASCP) Memorial Regional Hospital 3501 Johnson Street Hollywood, FL 33021 Ph: 954-265-5317 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Houston, Ronald [Ronald.Houston@nationwidechildrens.org] Sent: Tuesday, June 25, 2013 3:41 PM To: joelle weaver; Susan Foreman; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HPV ISH Probes Many people are working on the Enzo probes just now. Mayo does have the probes up and running on the Bonds, but are keeping tight-lipped about their protocols. We tried the Dako probes but never could get consistent results. Ronnie Houston MS HT(ASCP)QIHC -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelle weaver Sent: Tuesday, June 25, 2013 3:34 PM To: Susan Foreman; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HPV ISH Probes HPV6, 11, 16 18, 31, 33 & 51 "screening' Joelle Weaver MAOM, HTL (ASCP) QIHC > From: sforeman@labpath.com > To: histonet@lists.utsouthwestern.edu > Date: Tue, 25 Jun 2013 14:10:59 -0400 > Subject: [Histonet] HPV ISH Probes > > Does anyone have HPV-ISH working? utilizing a vendor for the probe > other than Ventana? Prep Kit? > > > > > > Many Thanks, > > Susan Foreman, HT (ASCP) > (865)584-1933 > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. ------------------------------------------------------------------------- This message was secured by ZixCorp(R). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CObregon <@t> mhs.net Wed Jun 26 12:47:34 2013 From: CObregon <@t> mhs.net (Obregon, Cecilia) Date: Wed Jun 26 12:48:34 2013 Subject: [Histonet] HPV ISH Probes In-Reply-To: References: Message-ID: <598BB4A3A92D7F4DAA14C30E4AE70C2F10B1B9E6@MHSEXMB05.mhs.net> What detection are you using? We only have Ventana instrumentation at our lab. Thank you Cecilia M. Obregon, HTL (ASCP) Memorial Regional Hospital 3501 Johnson Street Hollywood, FL 33021 954-265-5317 ________________________________ From: margaret chadwell [m_chadwell@hotmail.com] Sent: Wednesday, June 26, 2013 10:49 AM To: Obregon, Cecilia; Houston, Ronald; joelle weaver; Susan Foreman; histonet@lists.utsouthwestern.edu; Bitting, Angela K. Subject: Re: [Histonet] HPV ISH Probes We have gotten good results with the following probes. http://www.panomics.com/products/rna-in-situ-analysis/viewrna-ish-tissue-assay/overview At first they were not working well but the rep come out for the day and went over them with us and not the results are beautiful and consistent. Margie Chadwell Sent from Windows Mail From: Bitting, Angela K. Sent: ?Wednesday?, ?June? ?26?, ?2013 ?6?:?28? ?AM To: Obregon, Cecilia, Houston, Ronald, joelle weaver, Susan Foreman, histonet@lists.utsouthwestern.edu I believe so, yes. This is the info I was given. Buy Biotin Antibody Rabbit Polyclonal: A150-109A from Bethyl Laboratories, Inc. 25043 West FM 1097, Montgomery, TX 77356, 1-800-338-9579 -----Original Message--http://www.panomics.com/products/rna-in-situ-analysis/viewrna-ish-tissue-assay/overview--- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Obregon, Cecilia Sent: Tuesday, June 25, 2013 4:33 PM To: Houston, Ronald; joelle weaver; Susan Foreman; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HPV ISH Probes I have personally tried the Dako probes on the Ventana platform but no luck so far. I have heard about the Enzo probes been succesfully worked up at other institutions. Does anybody know if a 'link antibody' is needed for the Enzo probes? Greatly appreciate any inputs. Cecilia M. Obregon, HTL (ASCP) Memorial Regional Hospital 3501 Johnson Street Hollywood, FL 33021 Ph: 954-265-5317 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Houston, Ronald [Ronald.Houston@nationwidechildrens.org] Sent: Tuesday, June 25, 2013 3:41 PM To: joelle weaver; Susan Foreman; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HPV ISH Probes Many people are working on the Enzo probes just now. Mayo does have the probes up and running on the Bonds, but are keeping tight-lipped about their protocols. We tried the Dako probes but never could get consistent results. Ronnie Houston MS HT(ASCP)QIHC -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelle weaver Sent: Tuesday, June 25, 2013 3:34 PM To: Susan Foreman; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HPV ISH Probes HPV6, 11, 16 18, 31, 33 & 51 "screening' Joelle Weaver MAOM, HTL (ASCP) QIHC > From: sforeman@labpath.com > To: histonet@lists.utsouthwestern.edu > Date: Tue, 25 Jun 2013 14:10:59 -0400 > Subject: [Histonet] HPV ISH Probes > > Does anyone have HPV-ISH working? utilizing a vendor for the probe > other than Ventana? Prep Kit? > > > > > > Many Thanks, > > Susan Foreman, HT (ASCP) > (865)584-1933 > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. ------------------------------------------------------------------------- This message was secured by ZixCorp(R). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ktuttle <@t> umm.edu Wed Jun 26 13:36:47 2013 From: ktuttle <@t> umm.edu (Kimberly Tuttle) Date: Wed Jun 26 13:37:02 2013 Subject: [Histonet] making 10 % EDTA Message-ID: <51CAFC7F0200001A00098794@gwia2.umm.edu> Hi Histonetters, I have purchased a liquid EDTA solution that is .25M. I need to make 10% EDTA. Do I just dilute it 1/10 with DI water? Thanks Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 Pager#5029 (410) 328-5558 (410) 328-5508 fax https://cf.umaryland.edu/freezer/promo_pbr.cfm This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. From PMonfils <@t> Lifespan.org Wed Jun 26 13:58:44 2013 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Wed Jun 26 13:59:10 2013 Subject: [Histonet] making 10 % EDTA In-Reply-To: <51CAFC7F0200001A00098794@gwia2.umm.edu> References: <51CAFC7F0200001A00098794@gwia2.umm.edu> Message-ID: The MW of EDTA is 294.2. A 1M solution would therefore contain 294.2 g/L, so a .25M solution would contain 73.55 g/L. A 10% solution would contain 100g/L, so your solution is already less concentrated than 10%. From dmccaig <@t> ckha.on.ca Wed Jun 26 14:01:41 2013 From: dmccaig <@t> ckha.on.ca (Diana McCaig) Date: Wed Jun 26 14:01:52 2013 Subject: [Histonet] Bone marrow blocks Message-ID: I sent out a request for information a while back and am going to ask again from a different approach. I am finding problems with embedding blocks of bone marrow particles with or without blood clot. Does anyone have a special technique they would like to share to concentrate the particles centrally in the block. Our current technique has the particles put in a block and then they are tamped down causing them to disperse throughout the block. Sometimes this results in the particles being located around the perimeter of the block with very little centrally positioned yielding extensive time to scan the entire slide because they are so spaced out. Do you let the sample clot or do you use anti-coagulant? Are the particles separated or are they mixed in with the blood? Any suggestions will be greatly appreciated Thanks Diana From SJMccabe <@t> drmc.org Wed Jun 26 14:18:43 2013 From: SJMccabe <@t> drmc.org (McCabe, Sara) Date: Wed Jun 26 14:22:23 2013 Subject: [Histonet] Cytology Staining In-Reply-To: References: Message-ID: <02AE2390303AAB43A823930EAD6B63AE668A86B9@ex07> I have a question for those of you that also may perform cytology specimens at your facility. How do you prevent carryover from one case to another? For example, we had obvious carryover from a pleural fluid onto an FNA of a thyroid case. This has never happened here (to our knowledge). Has anyone else every experienced this? We spray fixed our slides with an alcohol/acetone based fixative before staining them. Any advice would be appreciated! Sara McCabe, HT(ASCP) DuBois Regional Medical Center This email and any attached files are confidential and intended solely for the intended recipient(s). If you are not the named recipient you should not read, distribute, copy or alter this email. Any views or opinions expressed in this email are those of the author and do not represent those of the company. Warning: Although precautions have been taken to make sure no viruses are present in this email, the company cannot accept responsibility for any loss or damage that arise from the use of this email or attachments. From TGoins <@t> mt.gov Wed Jun 26 14:46:49 2013 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Wed Jun 26 14:47:25 2013 Subject: [Histonet] making 10 % EDTA In-Reply-To: <51CAFC7F0200001A00098794@gwia2.umm.edu> References: <51CAFC7F0200001A00098794@gwia2.umm.edu> Message-ID: The molecular weight of the EDTA will vary depending on chemical makeup - regardless, the solution you have is probably less than 10% already. EDTA tri-sodium salt with MW 358 at 0.25 M is 89.5 g per liter or 8.95 g per 100 ml or 8.95%. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kimberly Tuttle Sent: Wednesday, June 26, 2013 12:37 PM To: histonet Subject: [Histonet] making 10 % EDTA Hi Histonetters, I have purchased a liquid EDTA solution that is .25M. I need to make 10% EDTA. Do I just dilute it 1/10 with DI water? Thanks Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 Pager#5029 (410) 328-5558 (410) 328-5508 fax https://cf.umaryland.edu/freezer/promo_pbr.cfm This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. From tony.henwood <@t> health.nsw.gov.au Wed Jun 26 18:20:11 2013 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Wed Jun 26 18:20:41 2013 Subject: [Histonet] RE: Bone marrow blocks In-Reply-To: References: Message-ID: <6D6BD1DE8A5571489398B392A38A71579D2880BD@xmdb04.nch.kids> Well you could try the warm agar approach: Agar Cell Block Method Reference: Olson NJ, Gogel HK, Williams WL, et al (1986) Processing of aspiration cytology samples: an alternative method. Acta Cytol 30:409-412. Solutions: 1. 3% Agar 2. Fixative Procedure: 1. Fix material in preferred fixative 2. Heat 3% agar in a 60oC oven until molten. 3. Centrifuge specimen and decant supernatant. 4. Add 3-4 drops molten agar (dependent on size of sample), gently mix 5. Allow to cool, add fixative, remove block and process. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Diana McCaig Sent: Thursday, 27 June 2013 5:02 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Bone marrow blocks I sent out a request for information a while back and am going to ask again from a different approach. I am finding problems with embedding blocks of bone marrow particles with or without blood clot. Does anyone have a special technique they would like to share to concentrate the particles centrally in the block. Our current technique has the particles put in a block and then they are tamped down causing them to disperse throughout the block. Sometimes this results in the particles being located around the perimeter of the block with very little centrally positioned yielding extensive time to scan the entire slide because they are so spaced out. Do you let the sample clot or do you use anti-coagulant? Are the particles separated or are they mixed in with the blood? Any suggestions will be greatly appreciated Thanks Diana _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From cindy.deriso <@t> yale.edu Wed Jun 26 19:43:57 2013 From: cindy.deriso <@t> yale.edu (DeRiso, Cynthia) Date: Wed Jun 26 19:44:05 2013 Subject: [Histonet] Histology lab supervisor opening, Connecticut Message-ID: HISTOLOGY SUPERVISOR Dermatopathology Laboratory of New England seeks Histology Supervisor to join our excellent team. The supervisor oversees all laboratory functions, with the primary role being participation with and supervision of histology technicians in all aspects of processing skin biopsies, including grossing, embedding, cutting and special staining. This individual will organize work flow and resources to ensure efficient and cost-effective operations. Assist with teaching and implementation of all policies and procedures. Order laboratory supplies and materials to maintain adequate inventory, and ensure ongoing compliance with all quality and accreditation organization requirements. Candidate must have at least an Associate's degree in a laboratory science, e.g., biology, chemistry, or medical laboratory technology (Bachelor's degree preferred) and HT or HTL certification. Dermatopathology Laboratory of New England is a thriving, private dermatopathology laboratory in operation for 18 years, staffed with four physicians and approximately ten supporting staff members. We have a pleasant work environment and nice setting and are located in central Connecticut, with easy access from major highways. Excellent pay and benefits including full medical and 401(k) profit sharing plan. Please email cover letter and resume to Chad Ward at chad.ward@dlne.us or fax to 203.630.2909. Chad A Ward, MBA Operations Manager Dermatopathology Laboratory of New England, P.C. 140 Green Road Meriden, CT 06450 Ph: (203) 630-2666 Fax: (203) 630-2909 Sent from my iPad From jburch01 <@t> gmail.com Wed Jun 26 20:09:22 2013 From: jburch01 <@t> gmail.com (Jim Burchette) Date: Wed Jun 26 20:09:27 2013 Subject: [Histonet] making 10 % EDTA In-Reply-To: References: <51CAFC7F0200001A00098794@gwia2.umm.edu> Message-ID: Don't forget EDTA doesn't fully go into solution until the pH approaches 8.0. You will need to prepare a sodium hydroxide solution for pH adjustment. On Wed, Jun 26, 2013 at 3:46 PM, Goins, Tresa wrote: > The molecular weight of the EDTA will vary depending on chemical makeup - > regardless, the solution you have is probably less than 10% already. EDTA > tri-sodium salt with MW 358 at 0.25 M is 89.5 g per liter or 8.95 g per 100 > ml or 8.95%. > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kimberly Tuttle > Sent: Wednesday, June 26, 2013 12:37 PM > To: histonet > Subject: [Histonet] making 10 % EDTA > > Hi Histonetters, > > I have purchased a liquid EDTA solution that is .25M. I need to make 10% > EDTA. Do I just dilute it 1/10 with DI water? > > > Thanks > > > Kimberly C. Tuttle HT (ASCP) > Pathology Biorepository and Research Core University of Maryland Room > NBW58, UMMC > 22 S. Greene St > Baltimore, MD 21201 > Pager#5029 > (410) 328-5558 > (410) 328-5508 fax > https://cf.umaryland.edu/freezer/promo_pbr.cfm > > > > > This e-mail and any accompanying attachments may be privileged, > confidential, contain protected health information about an identified > patient or be otherwise protected from disclosure. State and federal law > protect the confidentiality of this information. If the reader of this > message is not the intended recipient; you are prohibited from using, > disclosing, reproducing or distributing this information; you should > immediately notify the sender by telephone or e-mail and delete this e-mail. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Jim Burchette "Fly Fishing Bum" *<'(((><* From TMcNemar <@t> lmhealth.org Thu Jun 27 04:47:53 2013 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Thu Jun 27 04:47:57 2013 Subject: [Histonet] RE: Cytology Staining In-Reply-To: <02AE2390303AAB43A823930EAD6B63AE668A86B9@ex07> References: <02AE2390303AAB43A823930EAD6B63AE668A86B9@ex07> Message-ID: We stain all fluids separately and check for malignancy. If malignant, the stains are changed before staining anything else. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of McCabe, Sara Sent: Wednesday, June 26, 2013 3:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cytology Staining I have a question for those of you that also may perform cytology specimens at your facility. How do you prevent carryover from one case to another? For example, we had obvious carryover from a pleural fluid onto an FNA of a thyroid case. This has never happened here (to our knowledge). Has anyone else every experienced this? We spray fixed our slides with an alcohol/acetone based fixative before staining them. Any advice would be appreciated! Sara McCabe, HT(ASCP) DuBois Regional Medical Center This email and any attached files are confidential and intended solely for the intended recipient(s). If you are not the named recipient you should not read, distribute, copy or alter this email. Any views or opinions expressed in this email are those of the author and do not represent those of the company. Warning: Although precautions have been taken to make sure no viruses are present in this email, the company cannot accept responsibility for any loss or damage that arise from the use of this email or attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From DKBoyd <@t> chs.net Thu Jun 27 09:13:00 2013 From: DKBoyd <@t> chs.net (Boyd, Debbie M) Date: Thu Jun 27 09:13:09 2013 Subject: [Histonet] RE: Cytology Staining In-Reply-To: <02AE2390303AAB43A823930EAD6B63AE668A86B9@ex07> References: <02AE2390303AAB43A823930EAD6B63AE668A86B9@ex07> Message-ID: <7EAFE982E328304DA6CE2B677BB76246794E4337@TN001WEXMBX12.US.chs.net> Body fluids are stained separately as they have a high potential for floating. FNA's , bronchs, thyroids, urine ect. Can all be stained together. After a malignant case is reported all stains are filtered and the first alcohol before each stain is discarded. The first alcohol after each stain is discarded and the others rotated up with a fresh one at the end. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of McCabe, Sara Sent: Wednesday, June 26, 2013 3:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cytology Staining I have a question for those of you that also may perform cytology specimens at your facility. How do you prevent carryover from one case to another? For example, we had obvious carryover from a pleural fluid onto an FNA of a thyroid case. This has never happened here (to our knowledge). Has anyone else every experienced this? We spray fixed our slides with an alcohol/acetone based fixative before staining them. Any advice would be appreciated! Sara McCabe, HT(ASCP) DuBois Regional Medical Center This email and any attached files are confidential and intended solely for the intended recipient(s). If you are not the named recipient you should not read, distribute, copy or alter this email. Any views or opinions expressed in this email are those of the author and do not represent those of the company. Warning: Although precautions have been taken to make sure no viruses are present in this email, the company cannot accept responsibility for any loss or damage that arise from the use of this email or attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From ktuttle <@t> umm.edu Thu Jun 27 09:57:55 2013 From: ktuttle <@t> umm.edu (Kimberly Tuttle) Date: Thu Jun 27 09:58:10 2013 Subject: [Histonet] making 10 % EDTA In-Reply-To: References: <51CAFC7F0200001A00098794@gwia2.umm.edu> Message-ID: <51CC1AB30200001A000988D6@gwia2.umm.edu> Thanks for your responses. Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 Pager#5029 (410) 328-5558 (410) 328-5508 fax https://cf.umaryland.edu/freezer/promo_pbr.cfm This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. From sforeman <@t> labpath.com Thu Jun 27 10:14:15 2013 From: sforeman <@t> labpath.com (Susan Foreman) Date: Thu Jun 27 10:16:51 2013 Subject: [Histonet] HPV ISH Probes In-Reply-To: <598BB4A3A92D7F4DAA14C30E4AE70C2F10B1B9E6@MHSEXMB05.mhs.net> References: <598BB4A3A92D7F4DAA14C30E4AE70C2F10B1B9E6@MHSEXMB05.mhs.net> Message-ID: <002901ce7348$ff909cb0$feb1d610$@com> Thank you all for your responses regarding HPV-ISH Probes. I am eagerly awaiting news that folks have this worked up on this platform and optimized. Kudos to you. Susan Foreman, HT (ASCP) KDL Pathology 315 Erin Drive Knoxville, TN 37919 (865)584-1933 From: Obregon, Cecilia [mailto:CObregon@mhs.net] Sent: Wednesday, June 26, 2013 1:48 PM To: margaret chadwell; Houston, Ronald; joelle weaver; Susan Foreman; histonet@lists.utsouthwestern.edu; Bitting, Angela K. Subject: RE: [Histonet] HPV ISH Probes What detection are you using? We only have Ventana instrumentation at our lab. Thank you Cecilia M. Obregon, HTL (ASCP) Memorial Regional Hospital 3501 Johnson Street Hollywood, FL 33021 954-265-5317 _____ From: margaret chadwell [m_chadwell@hotmail.com] Sent: Wednesday, June 26, 2013 10:49 AM To: Obregon, Cecilia; Houston, Ronald; joelle weaver; Susan Foreman; histonet@lists.utsouthwestern.edu; Bitting, Angela K. Subject: Re: [Histonet] HPV ISH Probes We have gotten good results with the following probes. http://www.panomics.com/products/rna-in-situ-analysis/viewrna-ish-tissue-ass ay/overview At first they were not working well but the rep come out for the day and went over them with us and not the results are beautiful and consistent. Margie Chadwell Sent from Windows Mail From: Bitting, Angela K. Sent: ?Wednesday?, ?June? ?26?, ?2013 ?6?:?28? ?AM To: Obregon, Cecilia, Houston, Ronald, joelle weaver, Susan Foreman, histonet@lists.utsouthwestern.edu I believe so, yes. This is the info I was given. Buy Biotin Antibody Rabbit Polyclonal: A150-109A from Bethyl Laboratories, Inc. 25043 West FM 1097, Montgomery, TX 77356, 1-800-338-9579 -----Original Message--http://www.panomics.com/products/rna-in-situ-analysis/viewrna-ish-t issue-assay/overview--- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Obregon, Cecilia Sent: Tuesday, June 25, 2013 4:33 PM To: Houston, Ronald; joelle weaver; Susan Foreman; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HPV ISH Probes I have personally tried the Dako probes on the Ventana platform but no luck so far. I have heard about the Enzo probes been succesfully worked up at other institutions. Does anybody know if a 'link antibody' is needed for the Enzo probes? Greatly appreciate any inputs. Cecilia M. Obregon, HTL (ASCP) Memorial Regional Hospital 3501 Johnson Street Hollywood, FL 33021 Ph: 954-265-5317 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Houston, Ronald [Ronald.Houston@nationwidechildrens.org] Sent: Tuesday, June 25, 2013 3:41 PM To: joelle weaver; Susan Foreman; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HPV ISH Probes Many people are working on the Enzo probes just now. Mayo does have the probes up and running on the Bonds, but are keeping tight-lipped about their protocols. We tried the Dako probes but never could get consistent results. Ronnie Houston MS HT(ASCP)QIHC -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelle weaver Sent: Tuesday, June 25, 2013 3:34 PM To: Susan Foreman; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HPV ISH Probes HPV6, 11, 16 18, 31, 33 & 51 "screening' Joelle Weaver MAOM, HTL (ASCP) QIHC > From: sforeman@labpath.com > To: histonet@lists.utsouthwestern.edu > Date: Tue, 25 Jun 2013 14:10:59 -0400 > Subject: [Histonet] HPV ISH Probes > > Does anyone have HPV-ISH working? utilizing a vendor for the probe > other than Ventana? Prep Kit? > > > > > > Many Thanks, > > Susan Foreman, HT (ASCP) > (865)584-1933 > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. ------------------------------------------------------------------------- This message was secured by ZixCorp(R). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CObregon <@t> mhs.net Thu Jun 27 10:22:45 2013 From: CObregon <@t> mhs.net (Obregon, Cecilia) Date: Thu Jun 27 10:23:40 2013 Subject: [Histonet] HPV ISH Probes In-Reply-To: <002901ce7348$ff909cb0$feb1d610$@com> References: <598BB4A3A92D7F4DAA14C30E4AE70C2F10B1B9E6@MHSEXMB05.mhs.net>, <002901ce7348$ff909cb0$feb1d610$@com> Message-ID: <598BB4A3A92D7F4DAA14C30E4AE70C2F10B1BB13@MHSEXMB05.mhs.net> Me too. I appreciate all of your input and suggestions. I have ordered the Enzo probes to try out. I will keep you posted. Cecilia M OBregon, HTL (ASCP) Memorial Regional Hospital 3501 Johnson Street Hollywood, FL 33021 954-265-5317 ________________________________ From: Susan Foreman [sforeman@labpath.com] Sent: Thursday, June 27, 2013 11:14 AM To: Obregon, Cecilia; 'margaret chadwell'; 'Houston, Ronald'; 'joelle weaver'; histonet@lists.utsouthwestern.edu; 'Bitting, Angela K.' Subject: RE: [Histonet] HPV ISH Probes Thank you all for your responses regarding HPV-ISH Probes. I am eagerly awaiting news that folks have this worked up on this platform and optimized. Kudos to you. Susan Foreman, HT (ASCP) KDL Pathology 315 Erin Drive Knoxville, TN 37919 (865)584-1933 From: Obregon, Cecilia [mailto:CObregon@mhs.net] Sent: Wednesday, June 26, 2013 1:48 PM To: margaret chadwell; Houston, Ronald; joelle weaver; Susan Foreman; histonet@lists.utsouthwestern.edu; Bitting, Angela K. Subject: RE: [Histonet] HPV ISH Probes What detection are you using? We only have Ventana instrumentation at our lab. Thank you Cecilia M. Obregon, HTL (ASCP) Memorial Regional Hospital 3501 Johnson Street Hollywood, FL 33021 954-265-5317 ________________________________ From: margaret chadwell [m_chadwell@hotmail.com] Sent: Wednesday, June 26, 2013 10:49 AM To: Obregon, Cecilia; Houston, Ronald; joelle weaver; Susan Foreman; histonet@lists.utsouthwestern.edu; Bitting, Angela K. Subject: Re: [Histonet] HPV ISH Probes We have gotten good results with the following probes. http://www.panomics.com/products/rna-in-situ-analysis/viewrna-ish-tissue-assay/overview At first they were not working well but the rep come out for the day and went over them with us and not the results are beautiful and consistent. Margie Chadwell Sent from Windows Mail From: Bitting, Angela K. Sent: ?Wednesday?, ?June? ?26?, ?2013 ?6?:?28? ?AM To: Obregon, Cecilia, Houston, Ronald, joelle weaver, Susan Foreman, histonet@lists.utsouthwestern.edu I believe so, yes. This is the info I was given. Buy Biotin Antibody Rabbit Polyclonal: A150-109A from Bethyl Laboratories, Inc. 25043 West FM 1097, Montgomery, TX 77356, 1-800-338-9579 -----Original Message--http://www.panomics.com/products/rna-in-situ-analysis/viewrna-ish-tissue-assay/overview--- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Obregon, Cecilia Sent: Tuesday, June 25, 2013 4:33 PM To: Houston, Ronald; joelle weaver; Susan Foreman; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HPV ISH Probes I have personally tried the Dako probes on the Ventana platform but no luck so far. I have heard about the Enzo probes been succesfully worked up at other institutions. Does anybody know if a 'link antibody' is needed for the Enzo probes? Greatly appreciate any inputs. Cecilia M. Obregon, HTL (ASCP) Memorial Regional Hospital 3501 Johnson Street Hollywood, FL 33021 Ph: 954-265-5317 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Houston, Ronald [Ronald.Houston@nationwidechildrens.org] Sent: Tuesday, June 25, 2013 3:41 PM To: joelle weaver; Susan Foreman; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HPV ISH Probes Many people are working on the Enzo probes just now. Mayo does have the probes up and running on the Bonds, but are keeping tight-lipped about their protocols. We tried the Dako probes but never could get consistent results. Ronnie Houston MS HT(ASCP)QIHC -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelle weaver Sent: Tuesday, June 25, 2013 3:34 PM To: Susan Foreman; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HPV ISH Probes HPV6, 11, 16 18, 31, 33 & 51 "screening' Joelle Weaver MAOM, HTL (ASCP) QIHC > From: sforeman@labpath.com > To: histonet@lists.utsouthwestern.edu > Date: Tue, 25 Jun 2013 14:10:59 -0400 > Subject: [Histonet] HPV ISH Probes > > Does anyone have HPV-ISH working? utilizing a vendor for the probe > other than Ventana? Prep Kit? > > > > > > Many Thanks, > > Susan Foreman, HT (ASCP) > (865)584-1933 > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. ------------------------------------------------------------------------- This message was secured by ZixCorp(R). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Thu Jun 27 13:55:04 2013 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Thu Jun 27 13:55:16 2013 Subject: [Histonet] HHV-8 Message-ID: <51CC5248020000770003C6DE@gwmail3.harthosp.org> Is anyone doing PCR for HHV-8 on formalin-fixed, paraffin-embedded tissue? We have a very interesting autopsy case where we would like to test brain tissue. Thank you! Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax From PMonfils <@t> Lifespan.org Thu Jun 27 13:58:31 2013 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Thu Jun 27 13:58:39 2013 Subject: [Histonet] pepsin pre-digestion before immuno staining Message-ID: We use trypsin and protease fairly often in our lab in conjunction with immuno staining, but we have not used pepsin. An investigator wants us to use pepsin, and we do have some, but would appreciate any recommendations regarding its use - concentration, time, temperature. Thanks Paul M. From Nancy_Schmitt <@t> pa-ucl.com Thu Jun 27 14:23:06 2013 From: Nancy_Schmitt <@t> pa-ucl.com (Nancy Schmitt) Date: Thu Jun 27 14:23:13 2013 Subject: [Histonet] Dragon users Message-ID: <906B4DA90ED1DB4DB6C7E94D7CEE6C36813D52E1@PEITHA.wad.pa-ucl.com> Hi All- we are using Dragon Enterprise and currently using Plantronics wireless headsets - does anyone have any experience with other wireless microphone options? Thanks for your input- NancySchmitt Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From portera <@t> msu.edu Thu Jun 27 14:32:17 2013 From: portera <@t> msu.edu (Amy Porter) Date: Thu Jun 27 14:32:09 2013 Subject: [Histonet] pepsin pre-digestion before immuno staining In-Reply-To: References: Message-ID: <000601ce736d$0901de70$1b059b50$@edu> 0.1% Trypsin + 0.1% Calcium Chloride in PBS for 10-20 minutes at 37C -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Monfils, Paul Sent: Thursday, June 27, 2013 2:59 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] pepsin pre-digestion before immuno staining We use trypsin and protease fairly often in our lab in conjunction with immuno staining, but we have not used pepsin. An investigator wants us to use pepsin, and we do have some, but would appreciate any recommendations regarding its use - concentration, time, temperature. Thanks Paul M. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From portera <@t> msu.edu Thu Jun 27 14:33:44 2013 From: portera <@t> msu.edu (Amy Porter) Date: Thu Jun 27 14:33:31 2013 Subject: [Histonet] pepsin pre-digestion before immuno staining In-Reply-To: References: Message-ID: <000701ce736d$3ca5c750$b5f155f0$@edu> Disregard my previous email that was for Trypsin.....duh, sorry about that!! Pepsin we use at a 0.04% in 0.2N HCl for 20 minutes at 37C -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Monfils, Paul Sent: Thursday, June 27, 2013 2:59 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] pepsin pre-digestion before immuno staining We use trypsin and protease fairly often in our lab in conjunction with immuno staining, but we have not used pepsin. An investigator wants us to use pepsin, and we do have some, but would appreciate any recommendations regarding its use - concentration, time, temperature. Thanks Paul M. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From susaninthelaboratory <@t> gmail.com Thu Jun 27 14:52:32 2013 From: susaninthelaboratory <@t> gmail.com (Susan Shaw) Date: Thu Jun 27 14:52:36 2013 Subject: [Histonet] Xylene contamination Message-ID: Hello, Is there something that can cause alcohol contamination of the first xylene on a VIP tissue processor, besides carryover or someone adding the wrong reagent to the xylene? Any help with this issue will be greatly appreciated. Thanks! From Rebecca.Riesen <@t> hma.com Fri Jun 28 09:32:54 2013 From: Rebecca.Riesen <@t> hma.com (Riesen, Rebecca) Date: Fri Jun 28 09:33:05 2013 Subject: [Histonet] RE: Histonet Digest, Vol 115, Issue 24 In-Reply-To: <19740412024625.D85D05230C33D7D5@mx2.hma.com> References: <19740412024625.D85D05230C33D7D5@mx2.hma.com> Message-ID: Message: 12 Date: Fri, 21 Jun 2013 09:49:13 -0600 From: JMaslanka@stpetes.org Subject: [Histonet] Billing ??? To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" Happy Friday This is a question to those who work at a hospital that have contracted pathologists. Does your hospital bill the technical component and the pathologist bill the profession component or does the pathologist bill everything and pay the hospital for technical component? Joe Maslanka BS, CT,HT (ASCP) Anatomical Pathology Technical Supervisor Give thanks for ALL things..... "Kindness is the language the blind can see & the deaf can hear- Mark Twain Here pathologists bill PC portion only. We (the hospital) bill the TC portion only. From rjbuesa <@t> yahoo.com Fri Jun 28 09:47:27 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jun 28 09:47:34 2013 Subject: [Histonet] RE: Histonet Digest, Vol 115, Issue 24 In-Reply-To: References: <19740412024625.D85D05230C33D7D5@mx2.hma.com> Message-ID: <1372430847.66733.YahooMailNeo@web163101.mail.bf1.yahoo.com> Depends on the contract signed between the PA and the hospital You can find both arrangements. Ren? J. From: "Riesen, Rebecca" To: "histonet@lists.utsouthwestern.edu" Sent: Friday, June 28, 2013 10:32 AM Subject: [Histonet] RE: Histonet Digest, Vol 115, Issue 24 Message: 12 Date: Fri, 21 Jun 2013 09:49:13 -0600 From: JMaslanka@stpetes.org Subject: [Histonet] Billing ??? To: histonet@lists.utsouthwestern.edu Message-ID: ??? ??? Content-Type: text/plain; charset="US-ASCII" Happy Friday This is a question to those who work at a hospital that have contracted pathologists. Does your hospital bill the technical component and the pathologist bill the profession component or does the pathologist bill everything and pay the hospital for technical component? Joe Maslanka BS, CT,HT (ASCP) Anatomical Pathology Technical Supervisor Give thanks for ALL things..... "Kindness is the language the blind can see & the deaf can hear- Mark Twain Here pathologists bill PC portion only.? We (the hospital) bill the TC portion only. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Valerie.Hannen <@t> parrishmed.com Fri Jun 28 11:02:31 2013 From: Valerie.Hannen <@t> parrishmed.com (Hannen, Valerie) Date: Fri Jun 28 11:02:38 2013 Subject: [Histonet] Disposable blade information Message-ID: <450B7A81EDA0C54E97C53D60F00776C3232B4BB60C@isexstore03> Good Morning all, I am having to do a cost analysis study for the cost effectiveness of Sharpening steel blades vs. transitioning to disposable blades. I know...what you all are thinking.. "Your're still using steel blades??"...Yes, we are... :). Our current knife sharpener has some issues, and I have decided to weigh buying a used knife sharpener against switching to the disposable system. So... a little background for you. We are a relatively small department, we average @ 50 blocks a day, of various tissues types. My questions to you are this...and I know that answers will only be estimations...but it is a place for me to start. What would be the average number of disposable blades that I would probably use in a day/ week? On an average...how many blocks can be cut on each blade? Has anyone recently made this transitiion? If so, what were some of the challenges?? I'm going to show my lack of knowledge of disposable blades with this next question...but here it goes.. What is the difference between a low profile blade and a high profile blade...and would I need to buy both?? Ok... my head is hurting...so.. I am going to leave you with all the above questions. Any and all information, suggestions, etc. will be greatly appreciated!! Thanks and have a GREAT day!! Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.hannen@parrishmed.com =================== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" =================== From modz9636 <@t> gmail.com Fri Jun 28 13:09:04 2013 From: modz9636 <@t> gmail.com (M.O.) Date: Fri Jun 28 13:09:08 2013 Subject: [Histonet] Decalcifier, adding Zinc Fixative Message-ID: Hello Histonet! Would anyone know if it is okay to add zinc fixative to TBD-2 decalcifier? What ratio would be okay? I am having an issue with human bone samples and want to make sure that these bones are properly fixed. They are about 7mm thick and I am fixing them for 4 days, but additional fixation in the TBD-2 would be best. Thank you, Merissa From TMcNemar <@t> lmhealth.org Fri Jun 28 13:16:29 2013 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Fri Jun 28 13:16:27 2013 Subject: [Histonet] RE: Disposable blade information In-Reply-To: <450B7A81EDA0C54E97C53D60F00776C3232B4BB60C@isexstore03> References: <450B7A81EDA0C54E97C53D60F00776C3232B4BB60C@isexstore03> Message-ID: I didn't think anybody still used steel knives.... I remember those days.... I would say to get away from the steel as soon as you can. Disposable will make your life so much easier. As you already know, it is near impossible to give you anything more than a guestimate but I would imagine that you could get by with one to three per day depending on case mix, staples, sutures, calcifications, etc. There are so many variables. It also depends on how many people are cutting those 50 blocks. There are a lot blades to choose from. You could probably get by for quite awhile just trying out different samples.... See if you can find a rep that can loan you a blade holder and try some. The holder will be the biggest upfront expense. I don't really know what the difference is between the high and low profiles but I suspect not much more than the height. I have tried many different brands over the years and always go back to the AccuEdge low profile. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie Sent: Friday, June 28, 2013 12:03 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Disposable blade information Good Morning all, I am having to do a cost analysis study for the cost effectiveness of Sharpening steel blades vs. transitioning to disposable blades. I know...what you all are thinking.. "Your're still using steel blades??"...Yes, we are... :). Our current knife sharpener has some issues, and I have decided to weigh buying a used knife sharpener against switching to the disposable system. So... a little background for you. We are a relatively small department, we average @ 50 blocks a day, of various tissues types. My questions to you are this...and I know that answers will only be estimations...but it is a place for me to start. What would be the average number of disposable blades that I would probably use in a day/ week? On an average...how many blocks can be cut on each blade? Has anyone recently made this transitiion? If so, what were some of the challenges?? I'm going to show my lack of knowledge of disposable blades with this next question...but here it goes.. What is the difference between a low profile blade and a high profile blade...and would I need to buy both?? Ok... my head is hurting...so.. I am going to leave you with all the above questions. Any and all information, suggestions, etc. will be greatly appreciated!! Thanks and have a GREAT day!! Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.hannen@parrishmed.com ============= "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ============= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From egray <@t> hsc.wvu.edu Fri Jun 28 13:15:21 2013 From: egray <@t> hsc.wvu.edu (Gray, Ed) Date: Fri Jun 28 13:20:02 2013 Subject: [Histonet] RE: Dragon users Message-ID: <0BBA94171603A6468FFBA03A45A029FC04441004B7@MSEXCL.hscresex.local> We use Sennheiser RF sets in the gross room. The sound quality is excellent and produce the best recognition of any wireless mike we've tried. These were pretty much the only wireless option Nuance would recommend when we first started using Dragon several years ago. The cons are: 1. The RF receiver is 21 x 10.5 x 3.5 cm with an external antenna and power supply, not huge but significant compared to a USB Bluetooth adapter. 2. You are wired to a transmitter which is not terribly large or heavy but a wire to your belt/pocket none-the-less. 3. They are battery hogs and quality drops rapidly once the indicator goes below 50%. 4. They were expensive when we bought them. I think they were over $300.00. We bought some replacement headsets at one point...also expensive. They have held up very well though. Ed Gray | Pathology IT Analyst | Phone: 304-293-2945 | Fax 304-293-1627 | WVU Healthcare l egray@wvuhealthcare.com Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ------------------------------ Message: 3 Date: Thu, 27 Jun 2013 19:23:06 +0000 From: Nancy Schmitt Subject: [Histonet] Dragon users To: "Histonet (histonet@lists.utsouthwestern.edu)" Message-ID: <906B4DA90ED1DB4DB6C7E94D7CEE6C36813D52E1@PEITHA.wad.pa-ucl.com> Content-Type: text/plain; charset="iso-8859-1" Hi All- we are using Dragon Enterprise and currently using Plantronics wireless headsets - does anyone have any experience with other wireless microphone options? Thanks for your input- NancySchmitt Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From Donna.Willis <@t> baylorhealth.edu Fri Jun 28 11:25:05 2013 From: Donna.Willis <@t> baylorhealth.edu (Willis, Donna G.) Date: Fri Jun 28 13:57:52 2013 Subject: [Histonet] PowerPath Issues Message-ID: <2572B4D63B62E64A8078D8BBE34D4078303DF1@BHDASVEXML2.bhcs.pvt> Anyone out there in Histoland having issues with PowerPath (Sunquest) customer service? Thanks, Donna Willis, HT/HTL (ASCP) Anatomic Pathology Manager Baylor University Medical Center-Dallas ph. 214-820-2465 office ph. 214-725-6184 mobile donna.willis@baylorhealth.edu ********************************************************************** This e-mail may contain confidential and/or privileged information. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. From JEllin <@t> yumaregional.org Fri Jun 28 14:01:19 2013 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Fri Jun 28 14:01:27 2013 Subject: [Histonet] PowerPath Issues In-Reply-To: <2572B4D63B62E64A8078D8BBE34D4078303DF1@BHDASVEXML2.bhcs.pvt> References: <2572B4D63B62E64A8078D8BBE34D4078303DF1@BHDASVEXML2.bhcs.pvt> Message-ID: Donna this is Jesus Ellin and I am the Sunquest POwerPath Chair,, I am cc Debbie Balli on this,, how can we help? Sent from my iPad On Jun 28, 2013, at 1:58 PM, "Willis, Donna G." wrote: > Anyone out there in Histoland having issues with PowerPath (Sunquest) customer service? > > Thanks, > > Donna Willis, HT/HTL (ASCP) > Anatomic Pathology Manager > Baylor University Medical Center-Dallas > ph. 214-820-2465 office > ph. 214-725-6184 mobile > donna.willis@baylorhealth.edu > > ********************************************************************** > This e-mail may contain confidential and/or privileged information. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ From christiegowan <@t> msn.com Fri Jun 28 14:36:46 2013 From: christiegowan <@t> msn.com (CHRISTIE GOWAN) Date: Fri Jun 28 14:36:50 2013 Subject: [Histonet] Alport Syndrome Message-ID: Is anyone using the Fluorchrome-Conjugated MoAbs for Alport Syndrome distributed by Cosmo Bio Co., LTD.? We are using it on cryostat sections of renal tissue and the Texas Red fluorochrome seems to be extremely robust while the FITC-conjugated-anti alpha2(IV) is weak. I would be interested to hear your outcomes/solutions. Christie Gowan HT (ASCP) UAB Hospital Surgical Pathology Supervisor Lab Services 205-934-4991 cgowan@uabmc.edu From Nathan.Ostlie <@t> ColoradoCollege.edu Fri Jun 28 15:17:18 2013 From: Nathan.Ostlie <@t> ColoradoCollege.edu (Nathan Ostlie (S)) Date: Fri Jun 28 15:17:26 2013 Subject: [Histonet] antibody providers for cytokine/chemokine IHC Message-ID: <8b449980f6f94ef684bd9dc45c3c7f44@BY2PR06MB012.namprd06.prod.outlook.com> Hello, I am new to immunohistochemistry and I am in the process of ordering primary antibody for cytokine/chemokine staining. Does anyone have recommendations for companies that sell antibody compatible with IHC? Examples of cytokines/chemokines we're hoping to stain for: CXCL5, CCL2, CXCL10, IL-6, IL-17 IFN-g Thanks, Nate From khbarr <@t> mdanderson.org Sat Jun 29 12:17:03 2013 From: khbarr <@t> mdanderson.org (Barr,Kaye H) Date: Sat Jun 29 12:17:17 2013 Subject: [Histonet] Re: Histonet Digest, Vol 115, Issue 32 In-Reply-To: <288bcb14-829b-486d-bc01-49872cc4b097@DCPWPEXHUBCAS04.mdanderson.edu> References: <288bcb14-829b-486d-bc01-49872cc4b097@DCPWPEXHUBCAS04.mdanderson.edu> Message-ID: <76C3EE12-49C6-49B9-9EF1-B9CE83D206E5@mdanderson.org> Sent from my iPad On Jun 29, 2013, at 12:03 PM, "histonet-request@lists.utsouthwestern.edu" wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Decalcifier, adding Zinc Fixative (M.O.) > 2. RE: Disposable blade information (Tom McNemar) > 3. RE: Dragon users (Gray, Ed) > 4. PowerPath Issues (Willis, Donna G.) > 5. Re: PowerPath Issues (Jesus Ellin) > 6. Alport Syndrome (CHRISTIE GOWAN) > 7. antibody providers for cytokine/chemokine IHC (Nathan Ostlie (S)) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Fri, 28 Jun 2013 11:09:04 -0700 > From: "M.O." > Subject: [Histonet] Decalcifier, adding Zinc Fixative > To: histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset=ISO-8859-1 > > Hello Histonet! Would anyone know if it is okay to add zinc fixative to > TBD-2 decalcifier? What ratio would be okay? > > I am having an issue with human bone samples and want to make sure that > these bones are properly fixed. They are about 7mm thick and I am fixing > them for 4 days, but additional fixation in the TBD-2 would be best. > > Thank you, > Merissa > > > ------------------------------ > > Message: 2 > Date: Fri, 28 Jun 2013 14:16:29 -0400 > From: Tom McNemar > Subject: [Histonet] RE: Disposable blade information > To: "'Hannen, Valerie'" , > "'histonet@lists.utsouthwestern.edu'" > > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > I didn't think anybody still used steel knives.... I remember those days.... > > I would say to get away from the steel as soon as you can. Disposable will make your life so much easier. > > As you already know, it is near impossible to give you anything more than a guestimate but I would imagine that you could get by with one to three per day depending on case mix, staples, sutures, calcifications, etc. There are so many variables. It also depends on how many people are cutting those 50 blocks. > > There are a lot blades to choose from. You could probably get by for quite awhile just trying out different samples.... > > See if you can find a rep that can loan you a blade holder and try some. The holder will be the biggest upfront expense. > > I don't really know what the difference is between the high and low profiles but I suspect not much more than the height. > > I have tried many different brands over the years and always go back to the AccuEdge low profile. > > Tom McNemar, HT(ASCP) > Histology Co-ordinator > Licking Memorial Health Systems > (740) 348-4163 > (740) 348-4166 > tmcnemar@lmhealth.org > www.LMHealth.org > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie > Sent: Friday, June 28, 2013 12:03 PM > To: 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] Disposable blade information > > Good Morning all, > > I am having to do a cost analysis study for the cost effectiveness of Sharpening steel blades vs. transitioning to disposable blades. I know...what you all are thinking.. > > "Your're still using steel blades??"...Yes, we are... :). Our current knife sharpener has some issues, and I have decided to weigh buying a used knife sharpener against > > switching to the disposable system. So... a little background for you. We are a relatively small department, we average @ 50 blocks a day, of various tissues types. > > My questions to you are this...and I know that answers will only be estimations...but it is a place for me to start. > > What would be the average number of disposable blades that I would probably use in a day/ week? On an average...how many blocks can be cut on each blade? > > Has anyone recently made this transitiion? If so, what were some of the challenges?? I'm going to show my lack of knowledge of disposable blades with this next > > question...but here it goes.. What is the difference between a low profile blade and a high profile blade...and would I need to buy both?? > > Ok... my head is hurting...so.. I am going to leave you with all the above questions. > > Any and all information, suggestions, etc. will be greatly appreciated!! > > Thanks and have a GREAT day!! > > Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) > Histology Section Chief > Parrish Medical Center > 951 N. Washington Ave. > Titusville, Florida 32976 > Phone:(321) 268-6333 ext. 7506 > Fax: (321) 268-6149 > valerie.hannen@parrishmed.com > > > > > > > > > ============= > "This email is intended solely for the use of the individual to > whom it is addressed and may contain information that is > privileged, confidential or otherwise exempt from disclosure > under applicable law. If the reader of this email is not the > intended recipient or the employee or agent responsible for > delivering the message to the intended recipient, you are > hereby notified that any dissemination, distribution, or > copying of this communication is strictly prohibited. If you > have received this communication in error, please immediately > delete this message. Thank you" > ============= > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. > > > > ------------------------------ > > Message: 3 > Date: Fri, 28 Jun 2013 14:15:21 -0400 > From: "Gray, Ed" > Subject: [Histonet] RE: Dragon users > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > <0BBA94171603A6468FFBA03A45A029FC04441004B7@MSEXCL.hscresex.local> > Content-Type: text/plain; charset="us-ascii" > > We use Sennheiser RF sets in the gross room. The sound quality is excellent and produce the best recognition of any wireless mike we've tried. These were pretty much the only wireless option Nuance would recommend when we first started using Dragon several years ago. The cons are: > 1. The RF receiver is 21 x 10.5 x 3.5 cm with an external antenna and power supply, not huge but significant compared to a USB Bluetooth adapter. > 2. You are wired to a transmitter which is not terribly large or heavy but a wire to your belt/pocket none-the-less. > 3. They are battery hogs and quality drops rapidly once the indicator goes below 50%. > 4. They were expensive when we bought them. I think they were over $300.00. We bought some replacement headsets at one point...also expensive. They have held up very well though. > > > > Ed Gray | Pathology IT Analyst | Phone: 304-293-2945 | Fax 304-293-1627 | WVU Healthcare l egray@wvuhealthcare.com > Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. > > ------------------------------ > > Message: 3 > Date: Thu, 27 Jun 2013 19:23:06 +0000 > From: Nancy Schmitt > Subject: [Histonet] Dragon users > To: "Histonet (histonet@lists.utsouthwestern.edu)" > > Message-ID: > <906B4DA90ED1DB4DB6C7E94D7CEE6C36813D52E1@PEITHA.wad.pa-ucl.com> > Content-Type: text/plain; charset="iso-8859-1" > > Hi All- > > we are using Dragon Enterprise and currently using Plantronics wireless headsets - does anyone have any experience with other wireless microphone options? > > Thanks for your input- > > NancySchmitt > > Dubuque, IA > > > > NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. > > > > > > > > > ------------------------------ > > Message: 4 > Date: Fri, 28 Jun 2013 16:25:05 +0000 > From: "Willis, Donna G." > Subject: [Histonet] PowerPath Issues > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > <2572B4D63B62E64A8078D8BBE34D4078303DF1@BHDASVEXML2.bhcs.pvt> > Content-Type: text/plain; charset="ISO-8859-1" > > Anyone out there in Histoland having issues with PowerPath (Sunquest) customer service? > > Thanks, > > Donna Willis, HT/HTL (ASCP) > Anatomic Pathology Manager > Baylor University Medical Center-Dallas > ph. 214-820-2465 office > ph. 214-725-6184 mobile > donna.willis@baylorhealth.edu > > ********************************************************************** > This e-mail may contain confidential and/or privileged information. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. > > > ------------------------------ > > Message: 5 > Date: Fri, 28 Jun 2013 19:01:19 +0000 > From: Jesus Ellin > Subject: Re: [Histonet] PowerPath Issues > To: "Willis, Donna G." > Cc: "histonet@lists.utsouthwestern.edu" > , Debbie Balli > > Message-ID: > Content-Type: text/plain; charset="us-ascii" > > Donna this is Jesus Ellin and I am the Sunquest POwerPath Chair,, I am cc Debbie Balli on this,, how can we help? > > Sent from my iPad > > On Jun 28, 2013, at 1:58 PM, "Willis, Donna G." wrote: > >> Anyone out there in Histoland having issues with PowerPath (Sunquest) customer service? >> >> Thanks, >> >> Donna Willis, HT/HTL (ASCP) >> Anatomic Pathology Manager >> Baylor University Medical Center-Dallas >> ph. 214-820-2465 office >> ph. 214-725-6184 mobile >> donna.willis@baylorhealth.edu >> >> ********************************************************************** >> This e-mail may contain confidential and/or privileged information. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ______________________________________________________________________ > This message is confidential, intended only for the named > recipient(s) and may contain information that is privileged > or exempt from disclosure under applicable law. If you are > not the intended recipient(s), you are notified that the > dissemination, distribution, or copying of this message is > strictly prohibited. If you receive this message in error, > or are not the named recipient(s), please notify the sender > at either the e-mail, fax, address, or telephone number > listed above and delete this e-mail from your computer. > Thank You. > ______________________________________________________________________ > > > > ------------------------------ > > Message: 6 > Date: Fri, 28 Jun 2013 19:36:46 +0000 > From: CHRISTIE GOWAN > Subject: [Histonet] Alport Syndrome > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > Is anyone using the Fluorchrome-Conjugated MoAbs for Alport Syndrome distributed by Cosmo Bio Co., LTD.? > > We are using it on cryostat sections of renal tissue and the Texas Red fluorochrome seems to be extremely robust while the FITC-conjugated-anti alpha2(IV) is weak. I would be interested to hear your outcomes/solutions. > > > > Christie Gowan HT (ASCP) > UAB Hospital Surgical Pathology > Supervisor Lab Services > 205-934-4991 > cgowan@uabmc.edu > > > > ------------------------------ > > Message: 7 > Date: Fri, 28 Jun 2013 20:17:18 +0000 > From: "Nathan Ostlie (S)" > Subject: [Histonet] antibody providers for cytokine/chemokine IHC > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > <8b449980f6f94ef684bd9dc45c3c7f44@BY2PR06MB012.namprd06.prod.outlook.com> > > Content-Type: text/plain; charset="us-ascii" > > Hello, > > I am new to immunohistochemistry and I am in the process of ordering primary antibody for cytokine/chemokine staining. Does anyone have recommendations for companies that sell antibody compatible with IHC? > > Examples of cytokines/chemokines we're hoping to stain for: CXCL5, CCL2, CXCL10, IL-6, IL-17 IFN-g > > Thanks, Nate > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 115, Issue 32 > *****************************************