[Histonet] Cryosectioning of Rabbit Arteries

[Rene J Buesa]

First you have to make sure they are round before they are placed in OCT. If they are not round at that step it will be very difficult to "round them up" in PBS.
If round in the OCT just pick them directly from the blade. Floating them is never a good idea.
René J. 


________________________________
From: Karie Durynski <durynski <@t> vet.upenn.edu>
To: histonet <@t> lists.utsouthwestern.edu 
Sent: Tuesday, July 30, 2013 11:15 AM
Subject: [Histonet] Cryosectioning of Rabbit Arteries


Good morning,
I am sectioning fresh frozen rabbit arteries and they need to be nice and round/oval on the slides before staining. I am trying to float them in PBS but losing the section in PBS and unable to pick up and place on the slide due to the size of the tissue. They are so small to handle this way. If any one has a procedure or any advice they can share it would be greatly appreciated.
Thank you much,
Karie

-- 
Karie L Durynski A.S.
New Bolton Center
University of Pennsylvania
School of Veterinary Medicine
Comparative Orthopaedic Research Laboratory
382 W Street Road
Kennett Square, PA. 19348
Phone:610-925-6278
Fax:610-925-6820
Email:durynski <@t> vet.upenn.edu

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