From amylee779 <@t> yahoo.com Mon Jul 1 13:05:50 2013 From: amylee779 <@t> yahoo.com (Amy Lee) Date: Mon Jul 1 13:05:55 2013 Subject: [Histonet] renin IHC Message-ID: <1372701950.60879.YahooMailNeo@web126006.mail.ne1.yahoo.com> Hello, I am looking for a renin antibody for IHC on FFPE rodent tissue. I searched around and couldn't?decide which one I should try.?Could anybody recommend one? ? Thanks in advance. ? Amy From Stacy_McLaughlin <@t> cooley-dickinson.org Mon Jul 1 13:38:30 2013 From: Stacy_McLaughlin <@t> cooley-dickinson.org (Stacy McLaughlin) Date: Mon Jul 1 13:38:37 2013 Subject: [Histonet] specimen tracking systems Message-ID: Hello Histoland, Wondering how many of you use specimen tracking systems (bar-coded) and what type of system you use. Pros-cons of each system? Thanks, Stacy From pkarlisch <@t> hmc.psu.edu Mon Jul 1 16:35:32 2013 From: pkarlisch <@t> hmc.psu.edu (Karlisch, Patricia) Date: Mon Jul 1 16:36:39 2013 Subject: [Histonet] Immunohistochemistry TAT Message-ID: Histonetters, I am looking for some help in getting IHC slides out in an 8 hour period of time. Here is some information. * We have a busy Immunohistochemistry lab and the volume of requests keep increasing. We offer approximately 150 different IHC stains ( including ISH and Dual ISH for Her 2). Generally, there are request for >150- 200 IHC tests twice a day, not including negative and positive (same slide) controls. * The techs pull an AM log at 6am and another at 1pm. The 1pm log will be handled while waiting for the IHC's to be completed from the 6am run and these will always be run overnight. * The 6am log requires that one tech start cutting each block for multiple IHC stains. (A search for blocks and the necessary control slide occurs by the same person). The slides are kept in a 60 degree oven for about 60 minutes. Most of these slides will be ready by 2-3:30pm. Here is my question. If we allowed requests for IHC stains to wait until 9AM how could we still get slides out by 3:30pm-4pm? In other words we would not have access to an LIS log until 9am so that the pathologists could order from the morning biopsies. What is the best way to do this with 3 Ventana Ultra's and one Benchmark XT. The blocks ( usually 40-50) need to be cut; sit in an oven and then get labeled and placed on the Ventana's. All longer stains such as ISH would be run overnight. The following tasks makes the 3:30- 4pm deadline difficult: * Searching for the blocks * Cutting the blocks (40-50) onto control slides + negative control * One hour in the oven to dry (Can we use 30minutes?) * Attaching labels to 150 slides and setting up instruments * One microtome * 2 techs/ some of the time Can you share your workflow with me if you do a similar volume of slides within an 8 hours period. How would you staff the two IHC techs to gain the most efficiency. As you know the fuller the instrument the longer the staining process. Thank you, Pat Karlisch, Supervisor pkarlisch@hmc.psu.edu Tel 717-531-6072 From lharris <@t> samhealth.org Mon Jul 1 16:45:27 2013 From: lharris <@t> samhealth.org (Lori Harris) Date: Mon Jul 1 16:45:33 2013 Subject: [Histonet] DAB Testing Message-ID: <450EDC37E404D142AF67D7314C954C8A3C7BCA943A@SHSMAILVI01.int.samhealth.net> Hello All! Can someone recommend a company that does DAB waste testing? I would like to get our waste tested from our Ventana Ultra. Thanks. Lori A. Harris, HT (ASCP) 541-768-6078 Histology Section Lead GSRMC Pathology Lab 3600 NW Samaritan Drive Corvallis, OR 97330 ________________________________ Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From lpwenk <@t> sbcglobal.net Tue Jul 2 04:55:03 2013 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Tue Jul 2 04:55:13 2013 Subject: [Histonet] If going to holiday/vacation, or to unsubscribe Message-ID: <837FE2645C9E46B4B740D031BA573AE2@HP2010> If you are going to vacation/holiday, please unsubscribe from Histonet. We don?t want a zillion messages saying ?I am out of the office.? If you no longer want to receive Histonet emails because it isn?t your cup of tea, then please follow these directions on how to unsubscribe: - Go to the bottom of any Histonet email (such as this one) - Click on the link to the webpage page (the one that starts with ?http?, not the email link) - Scroll again to the bottom of the page, type your email address where you receive Histonet, into the last box on the bottom of the page. - Hit the button that says ?unsubscribe or edit options? - Follow any other directions (sorry, I?m not going any further on the links, since I don?t want to unsubscribe. There may or may not be more directions. I don?t remember.) When back from your vacation/holiday, do a search for ?Histonet subscribe?, follow the links, and resubscribe. Or save this email, and go to: http://lists.utsouthwestern.edu/mailman/listinfo/histonet to resubscribe. Thank you. Peggy A. Wenk, HTL(ASCP)SLS From gu.lang <@t> gmx.at Tue Jul 2 06:43:36 2013 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Tue Jul 2 06:44:27 2013 Subject: AW: [Histonet] Immunohistochemistry TAT In-Reply-To: References: Message-ID: <001301ce7719$6459fd70$2d0df850$@gmx.at> Dear Pat, here some inputs. If usefull, you have to decide for yourself. Ad controls: We use for most of our antibodies an "universal-control". This is put together with tiny pieces of normal tonsil, appendix, skin, liver and pancreas. If you are interested, look at the website of NordiQC. Some labs prefer to cut the controls in advance. This can also save time, but the controls shouldn't get older than a week. Ad speed: we cut the urgent cases in advance besides the HE with 6 slides per block. These are needle biopsies from breast, liver, kidney, .... etc.; the pathologists only have to order a panel. Ad drying: we often skip the drying in the oven at all. But it is important to get rid of the water under the sections. (Besides: drying at high temp for longer period can alter stainability.) Ad LIS: we also have no LIS, but a light-version of it. We use a WORD-document as form. The pathologists can mark the wished antibodies and print the sheet directly onto our printer in the lab. Ad organisation: isn't it possible with 4 instruments to keep the same antibodies on each instrument? For example: one machine is the breast-machine, one for lymphomas, etc. If this is possible, you can add a "machine-code" on the labels and only look at this code while filling the machines. We work only with one Ultra. First technician comes at 6.30 and starts the first run until 7.00-7.30. Second run is started at 10.30-11.00 and the run over night is started around 15.00. The most slides have to been cut at afternoon. And if there are more than 30, they go into the morning run. This gives for the urgent biopsies an IHC-TAT about 5-6 hours (from HE delivering to IHC delivering). Most other cases are ready over night (HE delivering till 12.30 and IHC-ordering-deadline at 14.30). Hope this helps Gudrun Lang Ltd. BMA Histolabor Akh Linz, Austria -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Karlisch, Patricia Gesendet: Montag, 01. Juli 2013 23:36 An: 'histonet@lists.utsouthwestern.edu' Betreff: [Histonet] Immunohistochemistry TAT Histonetters, I am looking for some help in getting IHC slides out in an 8 hour period of time. Here is some information. * We have a busy Immunohistochemistry lab and the volume of requests keep increasing. We offer approximately 150 different IHC stains ( including ISH and Dual ISH for Her 2). Generally, there are request for >150- 200 IHC tests twice a day, not including negative and positive (same slide) controls. * The techs pull an AM log at 6am and another at 1pm. The 1pm log will be handled while waiting for the IHC's to be completed from the 6am run and these will always be run overnight. * The 6am log requires that one tech start cutting each block for multiple IHC stains. (A search for blocks and the necessary control slide occurs by the same person). The slides are kept in a 60 degree oven for about 60 minutes. Most of these slides will be ready by 2-3:30pm. Here is my question. If we allowed requests for IHC stains to wait until 9AM how could we still get slides out by 3:30pm-4pm? In other words we would not have access to an LIS log until 9am so that the pathologists could order from the morning biopsies. What is the best way to do this with 3 Ventana Ultra's and one Benchmark XT. The blocks ( usually 40-50) need to be cut; sit in an oven and then get labeled and placed on the Ventana's. All longer stains such as ISH would be run overnight. The following tasks makes the 3:30- 4pm deadline difficult: * Searching for the blocks * Cutting the blocks (40-50) onto control slides + negative control * One hour in the oven to dry (Can we use 30minutes?) * Attaching labels to 150 slides and setting up instruments * One microtome * 2 techs/ some of the time Can you share your workflow with me if you do a similar volume of slides within an 8 hours period. How would you staff the two IHC techs to gain the most efficiency. As you know the fuller the instrument the longer the staining process. Thank you, Pat Karlisch, Supervisor pkarlisch@hmc.psu.edu Tel 717-531-6072 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LSebree <@t> uwhealth.org Tue Jul 2 07:41:37 2013 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Tue Jul 2 07:41:43 2013 Subject: [Histonet] RE: Immunohistochemistry TAT In-Reply-To: References: Message-ID: <77DD817201982748BC67D7960F2F76AF0505C7@UWHC-MBX12.uwhis.hosp.wisc.edu> Pat, Right off the bat I can see 2 things that could speed things up for you. We have Ultras and only dry our slides for 10 minutes at 60 d C. If tissues don't stay on well, try some "Stay On" in your water bath; we're experimenting with that now. Also, we spend way too much time ourselves searching for blocks. See if there is a way to have them organized immediately after sectioning the H&Es so they are quicker to find...we are struggling with this. My 2 cents; good luck. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Karlisch, Patricia Sent: Monday, July 01, 2013 4:36 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Immunohistochemistry TAT Histonetters, I am looking for some help in getting IHC slides out in an 8 hour period of time. Here is some information. * We have a busy Immunohistochemistry lab and the volume of requests keep increasing. We offer approximately 150 different IHC stains ( including ISH and Dual ISH for Her 2). Generally, there are request for >150- 200 IHC tests twice a day, not including negative and positive (same slide) controls. * The techs pull an AM log at 6am and another at 1pm. The 1pm log will be handled while waiting for the IHC's to be completed from the 6am run and these will always be run overnight. * The 6am log requires that one tech start cutting each block for multiple IHC stains. (A search for blocks and the necessary control slide occurs by the same person). The slides are kept in a 60 degree oven for about 60 minutes. Most of these slides will be ready by 2-3:30pm. Here is my question. If we allowed requests for IHC stains to wait until 9AM how could we still get slides out by 3:30pm-4pm? In other words we would not have access to an LIS log until 9am so that the pathologists could order from the morning biopsies. What is the best way to do this with 3 Ventana Ultra's and one Benchmark XT. The blocks ( usually 40-50) need to be cut; sit in an oven and then get labeled and placed on the Ventana's. All longer stains such as ISH would be run overnight. The following tasks makes the 3:30- 4pm deadline difficult: * Searching for the blocks * Cutting the blocks (40-50) onto control slides + negative control * One hour in the oven to dry (Can we use 30minutes?) * Attaching labels to 150 slides and setting up instruments * One microtome * 2 techs/ some of the time Can you share your workflow with me if you do a similar volume of slides within an 8 hours period. How would you staff the two IHC techs to gain the most efficiency. As you know the fuller the instrument the longer the staining process. Thank you, Pat Karlisch, Supervisor pkarlisch@hmc.psu.edu Tel 717-531-6072 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brett_connolly <@t> merck.com Tue Jul 2 08:07:42 2013 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Tue Jul 2 08:08:52 2013 Subject: [Histonet] H&E staining question Message-ID: Histonetters - We had some rat tissues that were removed at necropsy and immediately fixed in formalin for 48 hrs. Since the tissues could not be processed after the 48 hr fixation they were transferred to 70% ETOH for about a week, then processed and stained with H&E. The H&E staining looks faded/washed out when compared to previous rat tissue that was fixed for 48 hr and then processed and stained without the extended time in 70% ETOH. The reason for limiting the fixation to 48 hr in the more recent study was that we were doing some IHC stains and wanted to keep the same fixation time as the previous study. I have extra unstained slides left over - any suggestions on how to get some better, crisper H&E results? Has anyone observed the washed out H&E appearance from tissues stored in 70% ETOH. Thanks, Brett Brett M. Connolly, Ph.D. Principal Scientist, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From brett_connolly <@t> merck.com Tue Jul 2 09:21:05 2013 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Tue Jul 2 09:21:12 2013 Subject: [Histonet] RE: H&E staining question - some clarification Message-ID: I forgot to mention that the H&E was performed in another lab at our facility...most likely on an autostainer. We just did the IHC studies and I have always used 70% ETOH for tissue storage ... never heard of this staining phenomenon happening before. Brett -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Connolly, Brett M Sent: Tuesday, July 02, 2013 9:08 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] H&E staining question Histonetters - We had some rat tissues that were removed at necropsy and immediately fixed in formalin for 48 hrs. Since the tissues could not be processed after the 48 hr fixation they were transferred to 70% ETOH for about a week, then processed and stained with H&E. The H&E staining looks faded/washed out when compared to previous rat tissue that was fixed for 48 hr and then processed and stained without the extended time in 70% ETOH. The reason for limiting the fixation to 48 hr in the more recent study was that we were doing some IHC stains and wanted to keep the same fixation time as the previous study. I have extra unstained slides left over - any suggestions on how to get some better, crisper H&E results? Has anyone observed the washed out H&E appearance from tissues stored in 70% ETOH. Thanks, Brett Brett M. Connolly, Ph.D. Principal Scientist, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From cforster <@t> umn.edu Tue Jul 2 09:50:02 2013 From: cforster <@t> umn.edu (Colleen Forster) Date: Tue Jul 2 09:50:14 2013 Subject: [Histonet] H&E staining question In-Reply-To: References: Message-ID: <51D2E89A.2070509@umn.edu> I am interested in the answer as I use this same technique on my rodent samples and have not had this issue. On 7/2/2013 8:07 AM, Connolly, Brett M wro > Histonetters - > > We had some rat tissues that were removed at necropsy and immediately fixed in formalin for 48 hrs. Since the tissues could not be processed after the 48 hr fixation they were transferred to 70% ETOH for about a week, then processed and stained with H&E. > > The H&E staining looks faded/washed out when compared to previous rat tissue that was fixed for 48 hr and then processed and stained without the extended time in 70% ETOH. The reason for limiting the fixation to 48 hr in the more recent study was that we were doing some IHC stains and wanted to keep the same fixation time as the previous study. > > I have extra unstained slides left over - any suggestions on how to get some better, crisper H&E results? Has anyone observed the washed out H&E appearance from tissues stored in 70% ETOH. > > Thanks, > Brett > > Brett M. Connolly, Ph.D. > Principal Scientist, Imaging Dept. > Merck & Co., Inc. > PO Box 4, WP-44K > West Point, PA 19486 > brett_connolly@merck.com > T- 215-652-2501 > F- 215-993-6803 > > > > > > > Notice: This e-mail message, together with any attachments, contains > information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, > New Jersey, USA 08889), and/or its affiliates Direct contact information > for affiliates is available at > http://www.merck.com/contact/contacts.html) that may be confidential, > proprietary copyrighted and/or legally privileged. It is intended solely > for the use of the individual or entity named on this message. If you are > not the intended recipient, and have received this message in error, > please notify us immediately by reply e-mail and then delete it from > your system. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BDeBrosse-Serra <@t> isisph.com Tue Jul 2 10:18:30 2013 From: BDeBrosse-Serra <@t> isisph.com (Bea DeBrosse-Serra) Date: Tue Jul 2 10:18:42 2013 Subject: [Histonet] RE: H&E staining question In-Reply-To: References: Message-ID: <493CAA64F203E14E8823737B9EE0E25F09488DD8A6@EXCHMB01.isis.local> Hi Brett, That the H&E staining looks faded after storage in 70% seems very unlikely. We do this with rat and mouse tissues all the time. Did the other lab use a different Hematoxylin, Eosin, or used a different staining protocol on the autostainer? Bea Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Connolly, Brett M Sent: Tuesday, July 02, 2013 6:08 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] H&E staining question Histonetters - We had some rat tissues that were removed at necropsy and immediately fixed in formalin for 48 hrs. Since the tissues could not be processed after the 48 hr fixation they were transferred to 70% ETOH for about a week, then processed and stained with H&E. The H&E staining looks faded/washed out when compared to previous rat tissue that was fixed for 48 hr and then processed and stained without the extended time in 70% ETOH. The reason for limiting the fixation to 48 hr in the more recent study was that we were doing some IHC stains and wanted to keep the same fixation time as the previous study. I have extra unstained slides left over - any suggestions on how to get some better, crisper H&E results? Has anyone observed the washed out H&E appearance from tissues stored in 70% ETOH. Thanks, Brett Brett M. Connolly, Ph.D. Principal Scientist, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LSebree <@t> uwhealth.org Tue Jul 2 11:00:21 2013 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Tue Jul 2 11:00:27 2013 Subject: [Histonet] IHC Elastin stain Message-ID: <77DD817201982748BC67D7960F2F76AF0506A0@UWHC-MBX12.uwhis.hosp.wisc.edu> Do any of you do this? Do any referral labs offer this? Thanks, Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 From Ronald.Houston <@t> nationwidechildrens.org Tue Jul 2 11:05:12 2013 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Tue Jul 2 11:05:19 2013 Subject: [Histonet] RE: IHC Elastin stain In-Reply-To: <77DD817201982748BC67D7960F2F76AF0506A0@UWHC-MBX12.uwhis.hosp.wisc.edu> References: <77DD817201982748BC67D7960F2F76AF0506A0@UWHC-MBX12.uwhis.hosp.wisc.edu> Message-ID: Linda, we do it here Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org "One person with passion is better than forty people merely interested." ~ E.M. Forster -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A Sent: Tuesday, July 02, 2013 12:00 PM To: Histonet (Histonet@lists.utsouthwestern.edu) Subject: [Histonet] IHC Elastin stain Do any of you do this? Do any referral labs offer this? Thanks, Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cpyse <@t> x-celllab.com Tue Jul 2 11:08:17 2013 From: cpyse <@t> x-celllab.com (Cindy Pyse) Date: Tue Jul 2 11:08:22 2013 Subject: [Histonet] DAB Testing In-Reply-To: <450EDC37E404D142AF67D7314C954C8A3C7BCA943A@SHSMAILVI01.int.samhealth.net> References: <450EDC37E404D142AF67D7314C954C8A3C7BCA943A@SHSMAILVI01.int.samhealth.net> Message-ID: <002c01ce773e$5db454a0$191cfde0$@x-celllab.com> I would be interested in this information as well. Cindy Cindy Pyse CLT, HT(ASCP) Laboratory Manager X-Cell Laboratories of WNY 20 Northpointe Parkway Ste 100 Amherst, NY 14228 716-250-9235 Ext. 232 cpyse@x-celllab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lori Harris Sent: Monday, July 01, 2013 5:45 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] DAB Testing Hello All! Can someone recommend a company that does DAB waste testing? I would like to get our waste tested from our Ventana Ultra. Thanks. Lori A. Harris, HT (ASCP) 541-768-6078 Histology Section Lead GSRMC Pathology Lab 3600 NW Samaritan Drive Corvallis, OR 97330 ________________________________ Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TJohnson <@t> gnf.org Tue Jul 2 12:24:08 2013 From: TJohnson <@t> gnf.org (Teri Johnson) Date: Tue Jul 2 12:24:14 2013 Subject: [Histonet] Re: H&E staining question Message-ID: <9F3CFEE76E51B64991C7485270890B404978C64C@EX4.lj.gnf.org> Hi Brett, I agree with the others, the storage in 70% ETOH is likely not the cause of the washed out appearance of the staining. * Did the lab that did the H&E staining run a daily control? How did that look? Perhaps the tap water they are using went a little funny? Perhaps the depar xylene needs refreshing? The hematoxylin is used up? If all the other H&Es done that day look good, then look further. * Look at the fixative, it is possible to get bad batches of that. It is also possible to cram a lot of tissue into a small space resulting in inadequate fixation. Or perhaps it was bloody and not changed to fresh fixative? Was one sample fixed at room temperature and the other fixed at 4 degrees C? Was the person doing the necropsy the same among animal batches? * Two things might help get better hematoxylin staining - I recall a journal article about using antigen retrieval pretreatment to improve H&E staining on samples that had been stored in fixative for a prolonged period of time. That might help. Years ago, I also noticed that the hematoxylin counterstained PAS slides looked better than our H&Es, so we put a bucket of 0.5% periodic acid as an oxidation step before hematoxylin. It needs to be rinsed well so there is no periodic acid carryover (that'll kill your hematoxylin!), but that might improve your staining. If you get this figured out, please let us know the cause and fix. Teri Johnson Manager, Histology GNF - San Diego, CA 858-332-4752 From JGarrigan <@t> cbiolabs.com Tue Jul 2 14:32:25 2013 From: JGarrigan <@t> cbiolabs.com (Jennifer Garrigan) Date: Tue Jul 2 14:32:51 2013 Subject: [Histonet] RE: Histonet Digest, Vol 116, Issue 1 In-Reply-To: <20130702170538.14C711BF7BA@barracuda.cbiolabs.com> References: <20130702170538.14C711BF7BA@barracuda.cbiolabs.com> Message-ID: <91BD8268D893C44A823BE2653A60CE5301A1C34D7A@cbiolabs05.CBiolabs.local> Re: H&E Staining We do not have a high volume of rat tissues and thus stain by hand. Generally we have increased the time in hematoxylin and eosin slightly because the tissue is so dense, even if cut at the same thickness as mouse tissue. We do long term storage in 70% EtOH and have not had any problems with staining after that. Hope this may help Jennifer -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Tuesday, July 02, 2013 1:06 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 116, Issue 1 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. renin IHC (Amy Lee) 2. specimen tracking systems (Stacy McLaughlin) 3. Immunohistochemistry TAT (Karlisch, Patricia) 4. DAB Testing (Lori Harris) 5. If going to holiday/vacation, or to unsubscribe (Lee & Peggy Wenk) 6. AW: [Histonet] Immunohistochemistry TAT (Gudrun Lang) 7. RE: Immunohistochemistry TAT (Sebree Linda A) 8. H&E staining question (Connolly, Brett M) 9. RE: H&E staining question - some clarification (Connolly, Brett M) 10. Re: H&E staining question (Colleen Forster) 11. RE: H&E staining question (Bea DeBrosse-Serra) 12. IHC Elastin stain (Sebree Linda A) 13. RE: IHC Elastin stain (Houston, Ronald) 14. RE: DAB Testing (Cindy Pyse) ---------------------------------------------------------------------- Message: 1 Date: Mon, 1 Jul 2013 11:05:50 -0700 (PDT) From: Amy Lee Subject: [Histonet] renin IHC To: histonet Message-ID: <1372701950.60879.YahooMailNeo@web126006.mail.ne1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hello, I am looking for a renin antibody for IHC on FFPE rodent tissue. I searched around and couldn't?decide which one I should try.?Could anybody recommend one? ? Thanks in advance. ? Amy ------------------------------ Message: 2 Date: Mon, 1 Jul 2013 18:38:30 +0000 From: Stacy McLaughlin Subject: [Histonet] specimen tracking systems To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Hello Histoland, Wondering how many of you use specimen tracking systems (bar-coded) and what type of system you use. Pros-cons of each system? Thanks, Stacy ------------------------------ Message: 3 Date: Mon, 1 Jul 2013 21:35:32 +0000 From: "Karlisch, Patricia" Subject: [Histonet] Immunohistochemistry TAT To: "'histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain; charset="us-ascii" Histonetters, I am looking for some help in getting IHC slides out in an 8 hour period of time. Here is some information. * We have a busy Immunohistochemistry lab and the volume of requests keep increasing. We offer approximately 150 different IHC stains ( including ISH and Dual ISH for Her 2). Generally, there are request for >150- 200 IHC tests twice a day, not including negative and positive (same slide) controls. * The techs pull an AM log at 6am and another at 1pm. The 1pm log will be handled while waiting for the IHC's to be completed from the 6am run and these will always be run overnight. * The 6am log requires that one tech start cutting each block for multiple IHC stains. (A search for blocks and the necessary control slide occurs by the same person). The slides are kept in a 60 degree oven for about 60 minutes. Most of these slides will be ready by 2-3:30pm. Here is my question. If we allowed requests for IHC stains to wait until 9AM how could we still get slides out by 3:30pm-4pm? In other words we would not have access to an LIS log until 9am so that the pathologists could order from the morning biopsies. What is the best way to do this with 3 Ventana Ultra's and one Benchmark XT. The blocks ( usually 40-50) need to be cut; sit in an oven and then get labeled and placed on the Ventana's. All longer stains such as ISH would be run overnight. The following tasks makes the 3:30- 4pm deadline difficult: * Searching for the blocks * Cutting the blocks (40-50) onto control slides + negative control * One hour in the oven to dry (Can we use 30minutes?) * Attaching labels to 150 slides and setting up instruments * One microtome * 2 techs/ some of the time Can you share your workflow with me if you do a similar volume of slides within an 8 hours period. How would you staff the two IHC techs to gain the most efficiency. As you know the fuller the instrument the longer the staining process. Thank you, Pat Karlisch, Supervisor pkarlisch@hmc.psu.edu Tel 717-531-6072 ------------------------------ Message: 4 Date: Mon, 1 Jul 2013 14:45:27 -0700 From: Lori Harris Subject: [Histonet] DAB Testing To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <450EDC37E404D142AF67D7314C954C8A3C7BCA943A@SHSMAILVI01.int.samhealth.net> Content-Type: text/plain; charset="us-ascii" Hello All! Can someone recommend a company that does DAB waste testing? I would like to get our waste tested from our Ventana Ultra. Thanks. Lori A. Harris, HT (ASCP) 541-768-6078 Histology Section Lead GSRMC Pathology Lab 3600 NW Samaritan Drive Corvallis, OR 97330 ________________________________ Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ------------------------------ Message: 5 Date: Tue, 2 Jul 2013 05:55:03 -0400 From: "Lee & Peggy Wenk" Subject: [Histonet] If going to holiday/vacation, or to unsubscribe To: "Histonet" Message-ID: <837FE2645C9E46B4B740D031BA573AE2@HP2010> Content-Type: text/plain; charset="utf-8" If you are going to vacation/holiday, please unsubscribe from Histonet. We don???t want a zillion messages saying ???I am out of the office.??? If you no longer want to receive Histonet emails because it isn???t your cup of tea, then please follow these directions on how to unsubscribe: - Go to the bottom of any Histonet email (such as this one) - Click on the link to the webpage page (the one that starts with ???http???, not the email link) - Scroll again to the bottom of the page, type your email address where you receive Histonet, into the last box on the bottom of the page. - Hit the button that says ???unsubscribe or edit options??? - Follow any other directions (sorry, I???m not going any further on the links, since I don???t want to unsubscribe. There may or may not be more directions. I don???t remember.) When back from your vacation/holiday, do a search for ???Histonet subscribe???, follow the links, and resubscribe. Or save this email, and go to: http://lists.utsouthwestern.edu/mailman/listinfo/histonet to resubscribe. Thank you. Peggy A. Wenk, HTL(ASCP)SLS ------------------------------ Message: 6 Date: Tue, 2 Jul 2013 13:43:36 +0200 From: "Gudrun Lang" Subject: AW: [Histonet] Immunohistochemistry TAT To: "'Karlisch, Patricia'" Cc: histonet@lists.utsouthwestern.edu Message-ID: <001301ce7719$6459fd70$2d0df850$@gmx.at> Content-Type: text/plain; charset="iso-8859-1" Dear Pat, here some inputs. If usefull, you have to decide for yourself. Ad controls: We use for most of our antibodies an "universal-control". This is put together with tiny pieces of normal tonsil, appendix, skin, liver and pancreas. If you are interested, look at the website of NordiQC. Some labs prefer to cut the controls in advance. This can also save time, but the controls shouldn't get older than a week. Ad speed: we cut the urgent cases in advance besides the HE with 6 slides per block. These are needle biopsies from breast, liver, kidney, .... etc.; the pathologists only have to order a panel. Ad drying: we often skip the drying in the oven at all. But it is important to get rid of the water under the sections. (Besides: drying at high temp for longer period can alter stainability.) Ad LIS: we also have no LIS, but a light-version of it. We use a WORD-document as form. The pathologists can mark the wished antibodies and print the sheet directly onto our printer in the lab. Ad organisation: isn't it possible with 4 instruments to keep the same antibodies on each instrument? For example: one machine is the breast-machine, one for lymphomas, etc. If this is possible, you can add a "machine-code" on the labels and only look at this code while filling the machines. We work only with one Ultra. First technician comes at 6.30 and starts the first run until 7.00-7.30. Second run is started at 10.30-11.00 and the run over night is started around 15.00. The most slides have to been cut at afternoon. And if there are more than 30, they go into the morning run. This gives for the urgent biopsies an IHC-TAT about 5-6 hours (from HE delivering to IHC delivering). Most other cases are ready over night (HE delivering till 12.30 and IHC-ordering-deadline at 14.30). Hope this helps Gudrun Lang Ltd. BMA Histolabor Akh Linz, Austria -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Karlisch, Patricia Gesendet: Montag, 01. Juli 2013 23:36 An: 'histonet@lists.utsouthwestern.edu' Betreff: [Histonet] Immunohistochemistry TAT Histonetters, I am looking for some help in getting IHC slides out in an 8 hour period of time. Here is some information. * We have a busy Immunohistochemistry lab and the volume of requests keep increasing. We offer approximately 150 different IHC stains ( including ISH and Dual ISH for Her 2). Generally, there are request for >150- 200 IHC tests twice a day, not including negative and positive >(same slide) controls. * The techs pull an AM log at 6am and another at 1pm. The 1pm log will be handled while waiting for the IHC's to be completed from the 6am run and these will always be run overnight. * The 6am log requires that one tech start cutting each block for multiple IHC stains. (A search for blocks and the necessary control slide occurs by the same person). The slides are kept in a 60 degree oven for about 60 minutes. Most of these slides will be ready by 2-3:30pm. Here is my question. If we allowed requests for IHC stains to wait until 9AM how could we still get slides out by 3:30pm-4pm? In other words we would not have access to an LIS log until 9am so that the pathologists could order from the morning biopsies. What is the best way to do this with 3 Ventana Ultra's and one Benchmark XT. The blocks ( usually 40-50) need to be cut; sit in an oven and then get labeled and placed on the Ventana's. All longer stains such as ISH would be run overnight. The following tasks makes the 3:30- 4pm deadline difficult: * Searching for the blocks * Cutting the blocks (40-50) onto control slides + negative control * One hour in the oven to dry (Can we use 30minutes?) * Attaching labels to 150 slides and setting up instruments * One microtome * 2 techs/ some of the time Can you share your workflow with me if you do a similar volume of slides within an 8 hours period. How would you staff the two IHC techs to gain the most efficiency. As you know the fuller the instrument the longer the staining process. Thank you, Pat Karlisch, Supervisor pkarlisch@hmc.psu.edu Tel 717-531-6072 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Tue, 2 Jul 2013 12:41:37 +0000 From: Sebree Linda A Subject: [Histonet] RE: Immunohistochemistry TAT To: "'Karlisch, Patricia'" , "'histonet@lists.utsouthwestern.edu'" Message-ID: <77DD817201982748BC67D7960F2F76AF0505C7@UWHC-MBX12.uwhis.hosp.wisc.edu> Content-Type: text/plain; charset="us-ascii" Pat, Right off the bat I can see 2 things that could speed things up for you. We have Ultras and only dry our slides for 10 minutes at 60 d C. If tissues don't stay on well, try some "Stay On" in your water bath; we're experimenting with that now. Also, we spend way too much time ourselves searching for blocks. See if there is a way to have them organized immediately after sectioning the H&Es so they are quicker to find...we are struggling with this. My 2 cents; good luck. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Karlisch, Patricia Sent: Monday, July 01, 2013 4:36 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Immunohistochemistry TAT Histonetters, I am looking for some help in getting IHC slides out in an 8 hour period of time. Here is some information. * We have a busy Immunohistochemistry lab and the volume of requests keep increasing. We offer approximately 150 different IHC stains ( including ISH and Dual ISH for Her 2). Generally, there are request for >150- 200 IHC tests twice a day, not including negative and positive (same slide) controls. * The techs pull an AM log at 6am and another at 1pm. The 1pm log will be handled while waiting for the IHC's to be completed from the 6am run and these will always be run overnight. * The 6am log requires that one tech start cutting each block for multiple IHC stains. (A search for blocks and the necessary control slide occurs by the same person). The slides are kept in a 60 degree oven for about 60 minutes. Most of these slides will be ready by 2-3:30pm. Here is my question. If we allowed requests for IHC stains to wait until 9AM how could we still get slides out by 3:30pm-4pm? In other words we would not have access to an LIS log until 9am so that the pathologists could order from the morning biopsies. What is the best way to do this with 3 Ventana Ultra's and one Benchmark XT. The blocks ( usually 40-50) need to be cut; sit in an oven and then get labeled and placed on the Ventana's. All longer stains such as ISH would be run overnight. The following tasks makes the 3:30- 4pm deadline difficult: * Searching for the blocks * Cutting the blocks (40-50) onto control slides + negative control * One hour in the oven to dry (Can we use 30minutes?) * Attaching labels to 150 slides and setting up instruments * One microtome * 2 techs/ some of the time Can you share your workflow with me if you do a similar volume of slides within an 8 hours period. How would you staff the two IHC techs to gain the most efficiency. As you know the fuller the instrument the longer the staining process. Thank you, Pat Karlisch, Supervisor pkarlisch@hmc.psu.edu Tel 717-531-6072 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Tue, 2 Jul 2013 09:07:42 -0400 From: "Connolly, Brett M" Subject: [Histonet] H&E staining question To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Histonetters - We had some rat tissues that were removed at necropsy and immediately fixed in formalin for 48 hrs. Since the tissues could not be processed after the 48 hr fixation they were transferred to 70% ETOH for about a week, then processed and stained with H&E. The H&E staining looks faded/washed out when compared to previous rat tissue that was fixed for 48 hr and then processed and stained without the extended time in 70% ETOH. The reason for limiting the fixation to 48 hr in the more recent study was that we were doing some IHC stains and wanted to keep the same fixation time as the previous study. I have extra unstained slides left over - any suggestions on how to get some better, crisper H&E results? Has anyone observed the washed out H&E appearance from tissues stored in 70% ETOH. Thanks, Brett Brett M. Connolly, Ph.D. Principal Scientist, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------ Message: 9 Date: Tue, 2 Jul 2013 10:21:05 -0400 From: "Connolly, Brett M" Subject: [Histonet] RE: H&E staining question - some clarification To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" I forgot to mention that the H&E was performed in another lab at our facility...most likely on an autostainer. We just did the IHC studies and I have always used 70% ETOH for tissue storage ... never heard of this staining phenomenon happening before. Brett -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Connolly, Brett M Sent: Tuesday, July 02, 2013 9:08 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] H&E staining question Histonetters - We had some rat tissues that were removed at necropsy and immediately fixed in formalin for 48 hrs. Since the tissues could not be processed after the 48 hr fixation they were transferred to 70% ETOH for about a week, then processed and stained with H&E. The H&E staining looks faded/washed out when compared to previous rat tissue that was fixed for 48 hr and then processed and stained without the extended time in 70% ETOH. The reason for limiting the fixation to 48 hr in the more recent study was that we were doing some IHC stains and wanted to keep the same fixation time as the previous study. I have extra unstained slides left over - any suggestions on how to get some better, crisper H&E results? Has anyone observed the washed out H&E appearance from tissues stored in 70% ETOH. Thanks, Brett Brett M. Connolly, Ph.D. Principal Scientist, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------ Message: 10 Date: Tue, 02 Jul 2013 09:50:02 -0500 From: Colleen Forster Subject: Re: [Histonet] H&E staining question To: histonet@lists.utsouthwestern.edu Message-ID: <51D2E89A.2070509@umn.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed I am interested in the answer as I use this same technique on my rodent samples and have not had this issue. On 7/2/2013 8:07 AM, Connolly, Brett M wro > Histonetters - > > We had some rat tissues that were removed at necropsy and immediately fixed in formalin for 48 hrs. Since the tissues could not be processed after the 48 hr fixation they were transferred to 70% ETOH for about a week, then processed and stained with H&E. > > The H&E staining looks faded/washed out when compared to previous rat tissue that was fixed for 48 hr and then processed and stained without the extended time in 70% ETOH. The reason for limiting the fixation to 48 hr in the more recent study was that we were doing some IHC stains and wanted to keep the same fixation time as the previous study. > > I have extra unstained slides left over - any suggestions on how to get some better, crisper H&E results? Has anyone observed the washed out H&E appearance from tissues stored in 70% ETOH. > > Thanks, > Brett > > Brett M. Connolly, Ph.D. > Principal Scientist, Imaging Dept. > Merck & Co., Inc. > PO Box 4, WP-44K > West Point, PA 19486 > brett_connolly@merck.com > T- 215-652-2501 > F- 215-993-6803 > > > > > > > Notice: This e-mail message, together with any attachments, contains > information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, > New Jersey, USA 08889), and/or its affiliates Direct contact information > for affiliates is available at > http://www.merck.com/contact/contacts.html) that may be confidential, > proprietary copyrighted and/or legally privileged. It is intended solely > for the use of the individual or entity named on this message. If you are > not the intended recipient, and have received this message in error, > please notify us immediately by reply e-mail and then delete it from > your system. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Tue, 2 Jul 2013 08:18:30 -0700 From: Bea DeBrosse-Serra Subject: [Histonet] RE: H&E staining question To: "'Connolly, Brett M'" , "histonet@lists.utsouthwestern.edu" Message-ID: <493CAA64F203E14E8823737B9EE0E25F09488DD8A6@EXCHMB01.isis.local> Content-Type: text/plain; charset="us-ascii" Hi Brett, That the H&E staining looks faded after storage in 70% seems very unlikely. We do this with rat and mouse tissues all the time. Did the other lab use a different Hematoxylin, Eosin, or used a different staining protocol on the autostainer? Bea Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Connolly, Brett M Sent: Tuesday, July 02, 2013 6:08 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] H&E staining question Histonetters - We had some rat tissues that were removed at necropsy and immediately fixed in formalin for 48 hrs. Since the tissues could not be processed after the 48 hr fixation they were transferred to 70% ETOH for about a week, then processed and stained with H&E. The H&E staining looks faded/washed out when compared to previous rat tissue that was fixed for 48 hr and then processed and stained without the extended time in 70% ETOH. The reason for limiting the fixation to 48 hr in the more recent study was that we were doing some IHC stains and wanted to keep the same fixation time as the previous study. I have extra unstained slides left over - any suggestions on how to get some better, crisper H&E results? Has anyone observed the washed out H&E appearance from tissues stored in 70% ETOH. Thanks, Brett Brett M. Connolly, Ph.D. Principal Scientist, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 12 Date: Tue, 2 Jul 2013 16:00:21 +0000 From: Sebree Linda A Subject: [Histonet] IHC Elastin stain To: "Histonet (Histonet@lists.utsouthwestern.edu)" Message-ID: <77DD817201982748BC67D7960F2F76AF0506A0@UWHC-MBX12.uwhis.hosp.wisc.edu> Content-Type: text/plain; charset="us-ascii" Do any of you do this? Do any referral labs offer this? Thanks, Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 ------------------------------ Message: 13 Date: Tue, 2 Jul 2013 16:05:12 +0000 From: "Houston, Ronald" Subject: [Histonet] RE: IHC Elastin stain To: Sebree Linda A , "Histonet (Histonet@lists.utsouthwestern.edu)" Message-ID: Content-Type: text/plain; charset="us-ascii" Linda, we do it here Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org "One person with passion is better than forty people merely interested." ~ E.M. Forster -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A Sent: Tuesday, July 02, 2013 12:00 PM To: Histonet (Histonet@lists.utsouthwestern.edu) Subject: [Histonet] IHC Elastin stain Do any of you do this? Do any referral labs offer this? Thanks, Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Tue, 2 Jul 2013 12:08:17 -0400 From: "Cindy Pyse" Subject: RE: [Histonet] DAB Testing To: "'Lori Harris'" , Message-ID: <002c01ce773e$5db454a0$191cfde0$@x-celllab.com> Content-Type: text/plain; charset="us-ascii" I would be interested in this information as well. Cindy Cindy Pyse CLT, HT(ASCP) Laboratory Manager X-Cell Laboratories of WNY 20 Northpointe Parkway Ste 100 Amherst, NY 14228 716-250-9235 Ext. 232 cpyse@x-celllab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lori Harris Sent: Monday, July 01, 2013 5:45 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] DAB Testing Hello All! Can someone recommend a company that does DAB waste testing? I would like to get our waste tested from our Ventana Ultra. Thanks. Lori A. Harris, HT (ASCP) 541-768-6078 Histology Section Lead GSRMC Pathology Lab 3600 NW Samaritan Drive Corvallis, OR 97330 ________________________________ Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 116, Issue 1 **************************************** This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information. From pruegg <@t> ihctech.net Tue Jul 2 17:22:50 2013 From: pruegg <@t> ihctech.net (pruegg@ihctech.net) Date: Tue Jul 2 17:22:53 2013 Subject: [Histonet] markers in specific species Message-ID: <008e01ce7772$b0f13d20$12d3b760$@ihctech.net> Can anyone recommend a good antibody made in rat that works in mouse tissue that is a nuclear marker and another one that is a membrane marker? Can anyone recommend a good antibody made in goat that works in mouse and rat tissue that is a nuclear marker and another one that is a membrane marker? Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E. Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 pruegghm@hotmail.com rueggihcconsultingpr@outlook.com This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. From tony.henwood <@t> health.nsw.gov.au Tue Jul 2 18:07:51 2013 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Tue Jul 2 18:08:08 2013 Subject: [Histonet] RE: Immunohistochemistry TAT In-Reply-To: <77DD817201982748BC67D7960F2F76AF0505C7@UWHC-MBX12.uwhis.hosp.wisc.edu> References: <77DD817201982748BC67D7960F2F76AF0505C7@UWHC-MBX12.uwhis.hosp.wisc.edu> Message-ID: <6D6BD1DE8A5571489398B392A38A71579D28918A@xmdb04.nch.kids> Yes, I agree with most. But remember a major part of dewaxing sections is to heat the sections to remove as much wax as possible. This allows the xylene or hydrocarbon dewaxing to be as efficient as it can. Remember to be wary of drying temperature, too high and some antigens (eg S100, 5D3, CMV) will be adversely affected (see Henwood, A., (2005) "Effect of Slide Drying at 80?C on Immunohistochemistry" J Histotechnol 28(1):45-46). We have noticed that staining for S100 was weaker on sections that had not been heated for as long as our routine time (30minutes 64oC). One wonders whether heated detergent dewaxing might be more efficient at removing wax (see Henwood, A. F., Prasad, L., & Bourke, V. M. (2013). "The application of heated detergent dewaxing and rehydration to techniques for the demonstration of fungi: a comparison to routine xylene-alcohol dewaxing" J Histotechnol 36(2):45-50) It is also possible that different waxes (especially in older, "cured" blocks) have different de-waxing requirements. Something to consider! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A Sent: Tuesday, 2 July 2013 10:42 PM To: 'Karlisch, Patricia'; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Immunohistochemistry TAT Pat, Right off the bat I can see 2 things that could speed things up for you. We have Ultras and only dry our slides for 10 minutes at 60 d C. If tissues don't stay on well, try some "Stay On" in your water bath; we're experimenting with that now. Also, we spend way too much time ourselves searching for blocks. See if there is a way to have them organized immediately after sectioning the H&Es so they are quicker to find...we are struggling with this. My 2 cents; good luck. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Karlisch, Patricia Sent: Monday, July 01, 2013 4:36 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Immunohistochemistry TAT Histonetters, I am looking for some help in getting IHC slides out in an 8 hour period of time. Here is some information. * We have a busy Immunohistochemistry lab and the volume of requests keep increasing. We offer approximately 150 different IHC stains ( including ISH and Dual ISH for Her 2). Generally, there are request for >150- 200 IHC tests twice a day, not including negative and positive (same slide) controls. * The techs pull an AM log at 6am and another at 1pm. The 1pm log will be handled while waiting for the IHC's to be completed from the 6am run and these will always be run overnight. * The 6am log requires that one tech start cutting each block for multiple IHC stains. (A search for blocks and the necessary control slide occurs by the same person). The slides are kept in a 60 degree oven for about 60 minutes. Most of these slides will be ready by 2-3:30pm. Here is my question. If we allowed requests for IHC stains to wait until 9AM how could we still get slides out by 3:30pm-4pm? In other words we would not have access to an LIS log until 9am so that the pathologists could order from the morning biopsies. What is the best way to do this with 3 Ventana Ultra's and one Benchmark XT. The blocks ( usually 40-50) need to be cut; sit in an oven and then get labeled and placed on the Ventana's. All longer stains such as ISH would be run overnight. The following tasks makes the 3:30- 4pm deadline difficult: * Searching for the blocks * Cutting the blocks (40-50) onto control slides + negative control * One hour in the oven to dry (Can we use 30minutes?) * Attaching labels to 150 slides and setting up instruments * One microtome * 2 techs/ some of the time Can you share your workflow with me if you do a similar volume of slides within an 8 hours period. How would you staff the two IHC techs to gain the most efficiency. As you know the fuller the instrument the longer the staining process. Thank you, Pat Karlisch, Supervisor pkarlisch@hmc.psu.edu Tel 717-531-6072 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From tony.henwood <@t> health.nsw.gov.au Tue Jul 2 19:16:51 2013 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Tue Jul 2 19:17:08 2013 Subject: [Histonet] RE: H&E staining question In-Reply-To: References: Message-ID: <6D6BD1DE8A5571489398B392A38A71579D2891DD@xmdb04.nch.kids> You could try 10 minutes treatment with periodic acid prior to H&E staining (Luna's technique I think) Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Connolly, Brett M Sent: Tuesday, 2 July 2013 11:08 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] H&E staining question Histonetters - We had some rat tissues that were removed at necropsy and immediately fixed in formalin for 48 hrs. Since the tissues could not be processed after the 48 hr fixation they were transferred to 70% ETOH for about a week, then processed and stained with H&E. The H&E staining looks faded/washed out when compared to previous rat tissue that was fixed for 48 hr and then processed and stained without the extended time in 70% ETOH. The reason for limiting the fixation to 48 hr in the more recent study was that we were doing some IHC stains and wanted to keep the same fixation time as the previous study. I have extra unstained slides left over - any suggestions on how to get some better, crisper H&E results? Has anyone observed the washed out H&E appearance from tissues stored in 70% ETOH. Thanks, Brett Brett M. Connolly, Ph.D. Principal Scientist, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From pkarlisch <@t> hmc.psu.edu Wed Jul 3 08:35:13 2013 From: pkarlisch <@t> hmc.psu.edu (Karlisch, Patricia) Date: Wed Jul 3 08:35:23 2013 Subject: [Histonet] RE: Immunohistochemistry TAT In-Reply-To: <6D6BD1DE8A5571489398B392A38A71579D28918A@xmdb04.nch.kids> References: <77DD817201982748BC67D7960F2F76AF0505C7@UWHC-MBX12.uwhis.hosp.wisc.edu> <6D6BD1DE8A5571489398B392A38A71579D28918A@xmdb04.nch.kids> Message-ID: Tony, Great information! We will consider keeping the dewaxing time in mind. What do you think of hotplate warming? Pat -----Original Message----- From: Tony Henwood (SCHN) [mailto:tony.henwood@health.nsw.gov.au] Sent: Tuesday, July 02, 2013 7:08 PM To: 'Sebree Linda A'; Karlisch, Patricia; 'histonet@lists.utsouthwestern.edu' Subject: RE: Immunohistochemistry TAT Yes, I agree with most. But remember a major part of dewaxing sections is to heat the sections to remove as much wax as possible. This allows the xylene or hydrocarbon dewaxing to be as efficient as it can. Remember to be wary of drying temperature, too high and some antigens (eg S100, 5D3, CMV) will be adversely affected (see Henwood, A., (2005) "Effect of Slide Drying at 80?C on Immunohistochemistry" J Histotechnol 28(1):45-46). We have noticed that staining for S100 was weaker on sections that had not been heated for as long as our routine time (30minutes 64oC). One wonders whether heated detergent dewaxing might be more efficient at removing wax (see Henwood, A. F., Prasad, L., & Bourke, V. M. (2013). "The application of heated detergent dewaxing and rehydration to techniques for the demonstration of fungi: a comparison to routine xylene-alcohol dewaxing" J Histotechnol 36(2):45-50) It is also possible that different waxes (especially in older, "cured" blocks) have different de-waxing requirements. Something to consider! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A Sent: Tuesday, 2 July 2013 10:42 PM To: 'Karlisch, Patricia'; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Immunohistochemistry TAT Pat, Right off the bat I can see 2 things that could speed things up for you. We have Ultras and only dry our slides for 10 minutes at 60 d C. If tissues don't stay on well, try some "Stay On" in your water bath; we're experimenting with that now. Also, we spend way too much time ourselves searching for blocks. See if there is a way to have them organized immediately after sectioning the H&Es so they are quicker to find...we are struggling with this. My 2 cents; good luck. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Karlisch, Patricia Sent: Monday, July 01, 2013 4:36 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Immunohistochemistry TAT Histonetters, I am looking for some help in getting IHC slides out in an 8 hour period of time. Here is some information. * We have a busy Immunohistochemistry lab and the volume of requests keep increasing. We offer approximately 150 different IHC stains ( including ISH and Dual ISH for Her 2). Generally, there are request for >150- 200 IHC tests twice a day, not including negative and positive (same slide) controls. * The techs pull an AM log at 6am and another at 1pm. The 1pm log will be handled while waiting for the IHC's to be completed from the 6am run and these will always be run overnight. * The 6am log requires that one tech start cutting each block for multiple IHC stains. (A search for blocks and the necessary control slide occurs by the same person). The slides are kept in a 60 degree oven for about 60 minutes. Most of these slides will be ready by 2-3:30pm. Here is my question. If we allowed requests for IHC stains to wait until 9AM how could we still get slides out by 3:30pm-4pm? In other words we would not have access to an LIS log until 9am so that the pathologists could order from the morning biopsies. What is the best way to do this with 3 Ventana Ultra's and one Benchmark XT. The blocks ( usually 40-50) need to be cut; sit in an oven and then get labeled and placed on the Ventana's. All longer stains such as ISH would be run overnight. The following tasks makes the 3:30- 4pm deadline difficult: * Searching for the blocks * Cutting the blocks (40-50) onto control slides + negative control * One hour in the oven to dry (Can we use 30minutes?) * Attaching labels to 150 slides and setting up instruments * One microtome * 2 techs/ some of the time Can you share your workflow with me if you do a similar volume of slides within an 8 hours period. How would you staff the two IHC techs to gain the most efficiency. As you know the fuller the instrument the longer the staining process. Thank you, Pat Karlisch, Supervisor pkarlisch@hmc.psu.edu Tel 717-531-6072 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From TJohnson <@t> gnf.org Wed Jul 3 09:37:42 2013 From: TJohnson <@t> gnf.org (Teri Johnson) Date: Wed Jul 3 09:37:47 2013 Subject: [Histonet] Re: H&E staining question In-Reply-To: <1217DDB3D7DE5E418E3D560A268EABD048E0ECA0@xmdb03.nch.kids> References: <9F3CFEE76E51B64991C7485270890B404978C64C@EX4.lj.gnf.org>, <1217DDB3D7DE5E418E3D560A268EABD048E0ECA0@xmdb03.nch.kids> Message-ID: <5F46D504-1CC1-419E-BFFE-C928C02AB642@gnf.org> Hi Linda, There is no journal article, merely an observation on my part many years ago. Teri On Jul 2, 2013, at 10:05 PM, "Linda Prasad (SCHN)" wrote: > Hi Teri, > Oxidising the slides in periodic acid prior to H&E improves the HE. Would really appreciate if you could please send me the reference for that article. Thank you Teri. > > > Linda Prasad, MSc, BSc > > Hospital Scientist | Histopathology > t: 02 9845 3316 | f: 02 9845 3318 | e: linda.prasad@health.nsw.gov.au | w: www.schn.health.nsw.gov.au > m: 0425 314 267 > > > > Cnr Hawkesbury Road and Hainsworth Street, Westmead > Locked Bag 4001, Westmead NSW 2145 > > P Please consider the environment before printing this email. > > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Teri Johnson [TJohnson@gnf.org] > Sent: Wednesday, 3 July 2013 3:24 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: H&E staining question > > Hi Brett, > > I agree with the others, the storage in 70% ETOH is likely not the cause of the washed out appearance of the staining. > > * Did the lab that did the H&E staining run a daily control? How did that look? Perhaps the tap water they are using went a little funny? Perhaps the depar xylene needs refreshing? The hematoxylin is used up? If all the other H&Es done that day look good, then look further. > > * Look at the fixative, it is possible to get bad batches of that. It is also possible to cram a lot of tissue into a small space resulting in inadequate fixation. Or perhaps it was bloody and not changed to fresh fixative? Was one sample fixed at room temperature and the other fixed at 4 degrees C? Was the person doing the necropsy the same among animal batches? > > * Two things might help get better hematoxylin staining - I recall a journal article about using antigen retrieval pretreatment to improve H&E staining on samples that had been stored in fixative for a prolonged period of time. That might help. Years ago, I also noticed that the hematoxylin counterstained PAS slides looked better than our H&Es, so we put a bucket of 0.5% periodic acid as an oxidation step before hematoxylin. It needs to be rinsed well so there is no periodic acid carryover (that'll kill your hematoxylin!), but that might improve your staining. > > If you get this figured out, please let us know the cause and fix. > > Teri Johnson > Manager, Histology > GNF - San Diego, CA > 858-332-4752 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ********************************************************************************* > This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. > > Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. > > This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. > ********************************************************************************* From pam <@t> dlcjax.com Wed Jul 3 10:20:37 2013 From: pam <@t> dlcjax.com (pam@dlcjax.com) Date: Wed Jul 3 10:20:46 2013 Subject: [Histonet] (no subject) Message-ID: <926995000.9097.1372864837435.JavaMail.mail@webmail04> Please delete me from emails. Thanks Pam Mathews, CDC Dermatology and Laser Center Orange Park, Florida 32073 Office Manager 904-276-4500 Office 904-276-4160 Fax 904-945-6845 Cell From tkngflght <@t> yahoo.com Wed Jul 3 11:40:58 2013 From: tkngflght <@t> yahoo.com (Cheryl) Date: Wed Jul 3 11:41:04 2013 Subject: [Histonet] HepB sAg control tissue Message-ID: <1372869658.81824.YahooMailNeo@web161903.mail.bf1.yahoo.com> Hi guys! ? We're having trouble sourcing a good tissue control for hepatitis b surface antigen.? Willing to trade- ? Anyone anyone? ? Thank you! Cheryl Kerry, HT(ASCP) Full Staff Inc. From tkngflght <@t> yahoo.com Wed Jul 3 12:05:31 2013 From: tkngflght <@t> yahoo.com (Cheryl) Date: Wed Jul 3 12:05:35 2013 Subject: [Histonet] looking for techs in Louisiana Message-ID: <1372871131.76358.YahooMailNeo@web161905.mail.bf1.yahoo.com> Hello everyone--happy July 4th! ? Two labs in SW Louisiana are looking for histotechs.? One needs PRN so anyone within driving distance who wants a pick-up job--give me a holler?? I'm doing this as a favor so it's not a recruiting thing--helping a friend. ? Also--My lab needs a night shift tech--possibly a lead position with IHC skills but we'll consider a good core routine?histotech as well.? We have fun, the lab is clean, with good equipment and reasonable workloads.?We even go out as a crew once in a while--a rare culture these days.?The most recent hire couldn't manage the shift (her first time on nights).? Also not a recruiting thing--it's my lab!! ? Referals are welcome--we'll take good care of your friends :) ? Thanks! ? Cheryl Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab by helping one GREAT?Tech at a time.? 281.852.9457?Office 800.756.3309?Phone & Fax? admin@fullstaff.org From TNMayer <@t> mdanderson.org Wed Jul 3 12:34:33 2013 From: TNMayer <@t> mdanderson.org (Mayer,Toysha N) Date: Wed Jul 3 12:34:39 2013 Subject: [Histonet] RE: H&E staining question Message-ID: <47E9B2C01DDDD94881EACD2DC44EBC88016481AE@D1PWPEXMBX05.mdanderson.edu> Contact Erika Fredenburg at HCI Sciences. She and her father may be able to help you. Her email is: efredenburgh@hcisciences.com Toysha N. Mayer, MBA, HT (ASCP) Instructor, Education Coordinator Program in Histotechnology School of Health Professions MD Anderson Cancer Center (713) 563-3481 tnmayer@mdanderson.org Message: 1 Date: Tue, 2 Jul 2013 17:24:08 +0000 From: Teri Johnson Subject: [Histonet] Re: H&E staining question To: "histonet@lists.utsouthwestern.edu" Message-ID: <9F3CFEE76E51B64991C7485270890B404978C64C@EX4.lj.gnf.org> Content-Type: text/plain; charset="us-ascii" Hi Brett, I agree with the others, the storage in 70% ETOH is likely not the cause of the washed out appearance of the staining. * Did the lab that did the H&E staining run a daily control? How did that look? Perhaps the tap water they are using went a little funny? Perhaps the depar xylene needs refreshing? The hematoxylin is used up? If all the other H&Es done that day look good, then look further. * Look at the fixative, it is possible to get bad batches of that. It is also possible to cram a lot of tissue into a small space resulting in inadequate fixation. Or perhaps it was bloody and not changed to fresh fixative? Was one sample fixed at room temperature and the other fixed at 4 degrees C? Was the person doing the necropsy the same among animal batches? * Two things might help get better hematoxylin staining - I recall a journal article about using antigen retrieval pretreatment to improve H&E staining on samples that had been stored in fixative for a prolonged period of time. That might help. Years ago, I also noticed that the hematoxylin counterstained PAS slides looked better than our H&Es, so we put a bucket of 0.5% periodic acid as an oxidation step before hematoxylin. It needs to be rinsed well so there is no periodic acid carryover (that'll kill your hematoxylin!), but that might improve your staining. If you get this figured out, please let us know the cause and fix. Teri Johnson Manager, Histology GNF - San Diego, CA 858-332-4752 From TJohnson <@t> gnf.org Wed Jul 3 12:39:38 2013 From: TJohnson <@t> gnf.org (Teri Johnson) Date: Wed Jul 3 12:39:44 2013 Subject: [Histonet] RE: Re: H&E staining question In-Reply-To: <1217DDB3D7DE5E418E3D560A268EABD048E0ECA0@xmdb03.nch.kids> References: <9F3CFEE76E51B64991C7485270890B404978C64C@EX4.lj.gnf.org> <1217DDB3D7DE5E418E3D560A268EABD048E0ECA0@xmdb03.nch.kids> Message-ID: <9F3CFEE76E51B64991C7485270890B404978D048@EX4.lj.gnf.org> Thanks to Tony Henwood for the nudge regarding Luna...I found a blurb about it, and John Kiernan references Luna's AFIP manual. Blurb: http://cshprotocols.cshlp.org/content/2008/7/pdb.top50.full Specific location on the page: http://cshprotocols.cshlp.org/content/2008/7/pdb.top50.full#xref-ref-31-1 Reference: Luna L.G., ed (1968) Manual of histologic staining methods of the Armed Forces Institute of Pathology (McGraw-Hill, New York), 3. ~Teri -----Original Message----- From: Linda Prasad (SCHN) [mailto:linda.prasad@health.nsw.gov.au] Sent: Tuesday, July 02, 2013 10:05 PM To: Teri Johnson; histonet@lists.utsouthwestern.edu Subject: RE: Re: H&E staining question Hi Teri, Oxidising the slides in periodic acid prior to H&E improves the HE. Would really appreciate if you could please send me the reference for that article. Thank you Teri. Linda Prasad, MSc, BSc Hospital Scientist | Histopathology t: 02 9845 3316 | f: 02 9845 3318 | e: linda.prasad@health.nsw.gov.au | w: www.schn.health.nsw.gov.au m: 0425 314 267 Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145 P Please consider the environment before printing this email. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Teri Johnson [TJohnson@gnf.org] Sent: Wednesday, 3 July 2013 3:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: H&E staining question Hi Brett, I agree with the others, the storage in 70% ETOH is likely not the cause of the washed out appearance of the staining. * Did the lab that did the H&E staining run a daily control? How did that look? Perhaps the tap water they are using went a little funny? Perhaps the depar xylene needs refreshing? The hematoxylin is used up? If all the other H&Es done that day look good, then look further. * Look at the fixative, it is possible to get bad batches of that. It is also possible to cram a lot of tissue into a small space resulting in inadequate fixation. Or perhaps it was bloody and not changed to fresh fixative? Was one sample fixed at room temperature and the other fixed at 4 degrees C? Was the person doing the necropsy the same among animal batches? * Two things might help get better hematoxylin staining - I recall a journal article about using antigen retrieval pretreatment to improve H&E staining on samples that had been stored in fixative for a prolonged period of time. That might help. Years ago, I also noticed that the hematoxylin counterstained PAS slides looked better than our H&Es, so we put a bucket of 0.5% periodic acid as an oxidation step before hematoxylin. It needs to be rinsed well so there is no periodic acid carryover (that'll kill your hematoxylin!), but that might improve your staining. If you get this figured out, please let us know the cause and fix. Teri Johnson Manager, Histology GNF - San Diego, CA 858-332-4752 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From Tony_Reilly <@t> health.qld.gov.au Wed Jul 3 18:06:09 2013 From: Tony_Reilly <@t> health.qld.gov.au (Tony Reilly) Date: Wed Jul 3 18:07:09 2013 Subject: [Histonet] Re: H&E staining question In-Reply-To: <5F46D504-1CC1-419E-BFFE-C928C02AB642@gnf.org> References: <9F3CFEE76E51B64991C7485270890B404978C64C@EX4.lj.gnf.org>, <1217DDB3D7DE5E418E3D560A268EABD048E0ECA0@xmdb03.nch.kids> <5F46D504-1CC1-419E-BFFE-C928C02AB642@gnf.org> Message-ID: <51D53B01.411C.0039.0@health.qld.gov.au> Hi Linda For a little more information (not an article) go to: http://www.ihcworld.com/royellis/problems/problem21.htm regards Tony Tony Reilly B.App.Sc. , M.Sc. Chief Scientist, Anatomical Pathology Pathology Queensland-PA Laboratory ________________________________________________ Health Services Support Agency | Department of Health Level 1, Building 15,Princess Alexandra Hospital Ipswich Road,WOOLLOONGABBA Qld4102 Ph: 07 3176 2412 Mob: 0402 139411 Fax: 07 3176 2930 Email: tony_reilly@health.qld.gov.au Web: www.health.qld.gov.au/qhcss/ >>> Teri Johnson 7/4/2013 12:37 am >>> Hi Linda, There is no journal article, merely an observation on my part many years ago. Teri On Jul 2, 2013, at 10:05 PM, "Linda Prasad (SCHN)" wrote: > Hi Teri, > Oxidising the slides in periodic acid prior to H&E improves the HE. Would really appreciate if you could please send me the reference for that article. Thank you Teri. > > > Linda Prasad, MSc, BSc > > Hospital Scientist | Histopathology > t: 02 9845 3316 | f: 02 9845 3318 | e: linda.prasad@health.nsw.gov.au | w: www.schn.health.nsw.gov.au > m: 0425 314 267 > > > > Cnr Hawkesbury Road and Hainsworth Street, Westmead > Locked Bag 4001, Westmead NSW 2145 > > P Please consider the environment before printing this email. > > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Teri Johnson [TJohnson@gnf.org] > Sent: Wednesday, 3 July 2013 3:24 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: H&E staining question > > Hi Brett, > > I agree with the others, the storage in 70% ETOH is likely not the cause of the washed out appearance of the staining. > > * Did the lab that did the H&E staining run a daily control? How did that look? Perhaps the tap water they are using went a little funny? Perhaps the depar xylene needs refreshing? The hematoxylin is used up? If all the other H&Es done that day look good, then look further. > > * Look at the fixative, it is possible to get bad batches of that. It is also possible to cram a lot of tissue into a small space resulting in inadequate fixation. Or perhaps it was bloody and not changed to fresh fixative? Was one sample fixed at room temperature and the other fixed at 4 degrees C? Was the person doing the necropsy the same among animal batches? > > * Two things might help get better hematoxylin staining - I recall a journal article about using antigen retrieval pretreatment to improve H&E staining on samples that had been stored in fixative for a prolonged period of time. That might help. Years ago, I also noticed that the hematoxylin counterstained PAS slides looked better than our H&Es, so we put a bucket of 0.5% periodic acid as an oxidation step before hematoxylin. It needs to be rinsed well so there is no periodic acid carryover (that'll kill your hematoxylin!), but that might improve your staining. > > If you get this figured out, please let us know the cause and fix. > > Teri Johnson > Manager, Histology > GNF - San Diego, CA > 858-332-4752 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ********************************************************************************* > This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. > > Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. > > This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. > ********************************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ********************************************************************************** From akemiat3377 <@t> yahoo.com Wed Jul 3 18:54:19 2013 From: akemiat3377 <@t> yahoo.com (Akemi Allison) Date: Wed Jul 3 18:54:26 2013 Subject: [Histonet] Re: H&E staining question In-Reply-To: <51D53B01.411C.0039.0@health.qld.gov.au> References: <9F3CFEE76E51B64991C7485270890B404978C64C@EX4.lj.gnf.org>, <1217DDB3D7DE5E418E3D560A268EABD048E0ECA0@xmdb03.nch.kids> <5F46D504-1CC1-419E-BFFE-C928C02AB642@gnf.org> <51D53B01.411C.0039.0@health.qld.gov.au> Message-ID: <1372895659.60631.YahooMailNeo@web140603.mail.bf1.yahoo.com> Tony:? I actually was going to suggest this. ?You beat me to it! ?I used to do GMA on 1.5 microns sections and did a Harris hematoxylin and eosin/phloxine routinely (by the way, this was not recommended back in 1980. Gills hematoxylin was the norm!).? To get the results I wanted, I would routinely oxidize the slides in 1% periodic acid?and rince in running tap water before staining in hematoxylin. ?the staining was intensified and was beautiful! ?I came up with this because my PAS's with hematoxylin counterstains looked better than routine H&E's. ?I figuired it was worth doing the GMA's routinely using?1% periodic acid since we didn't do that many. Funny how we stumble on our modifications! ? Science never sleeps!! ? Akemi Allison BS, HT(ASCP)HTL Director Phoenix Lab Consulting E-Mail: akemiat3377@yahoo.com ________________________________ From: Tony Reilly To: Teri Johnson ; Linda Prasad (SCHN) Cc: "histonet@lists.utsouthwestern.edu" Sent: Wednesday, July 3, 2013 4:06 PM Subject: [Histonet] Re: H&E staining question Hi Linda For a little more information (not an article) go to: http://www.ihcworld.com/royellis/problems/problem21.htm regards Tony Tony Reilly? B.App.Sc. , M.Sc. Chief Scientist, Anatomical Pathology Pathology Queensland-PA Laboratory ________________________________________________ Health Services Support Agency | Department of Health Level 1, Building 15,Princess Alexandra Hospital Ipswich Road,WOOLLOONGABBA? Qld4102 Ph: 07 3176 2412 Mob: 0402 139411 Fax: 07 3176 2930 Email: tony_reilly@health.qld.gov.au Web:? www.health.qld.gov.au/qhcss/ >>> Teri Johnson 7/4/2013 12:37 am >>> Hi Linda, There is no journal article, merely an observation on my part many years ago. Teri On Jul 2, 2013, at 10:05 PM, "Linda Prasad (SCHN)" wrote: > Hi Teri, > Oxidising the slides in periodic acid prior to H&E improves the HE. Would really appreciate if you could please send me the reference for that article. Thank you Teri. > > > Linda Prasad, MSc, BSc > > Hospital Scientist | Histopathology > t: 02 9845 3316 | f: 02 9845 3318 | e: linda.prasad@health.nsw.gov.au | w: www.schn.health.nsw.gov.au > m: 0425 314 267 > > > > Cnr Hawkesbury Road and Hainsworth Street, Westmead > Locked Bag 4001, Westmead NSW 2145 > > P? Please consider the environment before printing this email. > > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Teri Johnson [TJohnson@gnf.org] > Sent: Wednesday, 3 July 2013 3:24 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: H&E staining question > > Hi Brett, > > I agree with the others, the storage in 70% ETOH is likely not the cause of the washed out appearance of the staining. > > *? ? ? Did the lab that did the H&E staining run a daily control? How did that look? Perhaps the tap water they are using went a little funny? Perhaps the depar xylene needs refreshing? The hematoxylin is used up? If all the other H&Es done that day look good, then look further. > > *? ? ? Look at the fixative, it is possible to get bad batches of that. It is also possible to cram a lot of tissue into a small space resulting in inadequate fixation. Or perhaps it was bloody and not changed to fresh fixative? Was one sample fixed at room temperature and the other fixed at 4 degrees C? Was the person doing the necropsy the same among animal batches? > > *? ? ? Two things might help get better hematoxylin staining - I recall a journal article about using antigen retrieval pretreatment to improve H&E staining on samples that had been stored in fixative for a prolonged period of time. That might help.? Years ago,? I also noticed that the hematoxylin counterstained PAS slides looked better than our H&Es, so we put a bucket of 0.5% periodic acid as an oxidation step before hematoxylin. It needs to be rinsed well so there is no periodic acid carryover (that'll kill your hematoxylin!), but that might improve your staining. > > If you get this figured out, please let us know the cause and fix. > > Teri Johnson > Manager, Histology > GNF - San Diego, CA > 858-332-4752 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ********************************************************************************* > This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. > > Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. > > This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. > ********************************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited.? The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email.? You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ********************************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akemiat3377 <@t> yahoo.com Wed Jul 3 19:06:25 2013 From: akemiat3377 <@t> yahoo.com (Akemi Allison) Date: Wed Jul 3 19:06:29 2013 Subject: [Histonet] Re: H&E staining question In-Reply-To: <1372895659.60631.YahooMailNeo@web140603.mail.bf1.yahoo.com> References: <9F3CFEE76E51B64991C7485270890B404978C64C@EX4.lj.gnf.org>, <1217DDB3D7DE5E418E3D560A268EABD048E0ECA0@xmdb03.nch.kids> <5F46D504-1CC1-419E-BFFE-C928C02AB642@gnf.org> <51D53B01.411C.0039.0@health.qld.gov.au> <1372895659.60631.YahooMailNeo@web140603.mail.bf1.yahoo.com> Message-ID: <1372896385.57994.YahooMailNeo@web140601.mail.bf1.yahoo.com> Oh by the way, the reason I did a?Harris hematoxylin and eosin/phloxine on GMA (plastics) was because our pathologists at OHSU hated Gills and were used to seeing Harris?hematoxylin on all of our regular surgical and autopsy slides. We only did GMA on kidney, liver, some GI and BM core bx's.? I was just a plain ole histologist that wanted to please her pathologists! ?Anything can be accomplished if you are creative and innovative! ? Akemi Allison BS, HT(ASCP)HTL Director Phoenix Lab Consulting E-Mail: akemiat3377@yahoo.com ________________________________ From: Akemi Allison To: Tony Reilly ; Teri Johnson ; Linda Prasad (SCHN) Cc: "histonet@lists.utsouthwestern.edu" Sent: Wednesday, July 3, 2013 4:54 PM Subject: Re: [Histonet] Re: H&E staining question Tony:? I actually was going to suggest this. ?You beat me to it! ?I used to do GMA on 1.5 microns sections and did a Harris hematoxylin and eosin/phloxine routinely (by the way, this was not recommended back in 1980. Gills hematoxylin was the norm!).? To get the results I wanted, I would routinely oxidize the slides in 1% periodic acid?and rince in running tap water before staining in hematoxylin. ?the staining was intensified and was beautiful! ?I came up with this because my PAS's with hematoxylin counterstains looked better than routine H&E's. ?I figuired it was worth doing the GMA's routinely using?1% periodic acid since we didn't do that many. Funny how we stumble on our modifications! ? Science never sleeps!! ? Akemi Allison BS, HT(ASCP)HTL Director Phoenix Lab Consulting E-Mail: akemiat3377@yahoo.com ________________________________ From: Tony Reilly To: Teri Johnson ; Linda Prasad (SCHN) Cc: "histonet@lists.utsouthwestern.edu" Sent: Wednesday, July 3, 2013 4:06 PM Subject: [Histonet] Re: H&E staining question Hi Linda For a little more information (not an article) go to: http://www.ihcworld.com/royellis/problems/problem21.htm regards Tony Tony Reilly? B.App.Sc. , M.Sc. Chief Scientist, Anatomical Pathology Pathology Queensland-PA Laboratory ________________________________________________ Health Services Support Agency | Department of Health Level 1, Building 15,Princess Alexandra Hospital Ipswich Road,WOOLLOONGABBA? Qld4102 Ph: 07 3176 2412 Mob: 0402 139411 Fax: 07 3176 2930 Email: tony_reilly@health.qld.gov.au Web:? www.health.qld.gov.au/qhcss/ >>> Teri Johnson 7/4/2013 12:37 am >>> Hi Linda, There is no journal article, merely an observation on my part many years ago. Teri On Jul 2, 2013, at 10:05 PM, "Linda Prasad (SCHN)" wrote: > Hi Teri, > Oxidising the slides in periodic acid prior to H&E improves the HE. Would really appreciate if you could please send me the reference for that article. Thank you Teri. > > > Linda Prasad, MSc, BSc > > Hospital Scientist | Histopathology > t: 02 9845 3316 | f: 02 9845 3318 | e: linda.prasad@health.nsw.gov.au | w: www.schn.health.nsw.gov.au > m: 0425 314 267 > > > > Cnr Hawkesbury Road and Hainsworth Street, Westmead > Locked Bag 4001, Westmead NSW 2145 > > P? Please consider the environment before printing this email. > > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Teri Johnson [TJohnson@gnf.org] > Sent: Wednesday, 3 July 2013 3:24 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: H&E staining question > > Hi Brett, > > I agree with the others, the storage in 70% ETOH is likely not the cause of the washed out appearance of the staining. > > *? ? ?? Did the lab that did the H&E staining run a daily control? How did that look? Perhaps the tap water they are using went a little funny? Perhaps the depar xylene needs refreshing? The hematoxylin is used up? If all the other H&Es done that day look good, then look further. > > *? ? ?? Look at the fixative, it is possible to get bad batches of that. It is also possible to cram a lot of tissue into a small space resulting in inadequate fixation. Or perhaps it was bloody and not changed to fresh fixative? Was one sample fixed at room temperature and the other fixed at 4 degrees C? Was the person doing the necropsy the same among animal batches? > > *? ? ?? Two things might help get better hematoxylin staining - I recall a journal article about using antigen retrieval pretreatment to improve H&E staining on samples that had been stored in fixative for a prolonged period of time. That might help.? Years ago,? I also noticed that the hematoxylin counterstained PAS slides looked better than our H&Es, so we put a bucket of 0.5% periodic acid as an oxidation step before hematoxylin. It needs to be rinsed well so there is no periodic acid carryover (that'll kill your hematoxylin!), but that might improve your staining. > > If you get this figured out, please let us know the cause and fix. > > Teri Johnson > Manager, Histology > GNF - San Diego, CA > 858-332-4752 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ********************************************************************************* > This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. > > Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. > > This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. > ********************************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited.? The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email.? You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ********************************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tony.henwood <@t> health.nsw.gov.au Wed Jul 3 19:38:06 2013 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Wed Jul 3 19:38:26 2013 Subject: [Histonet] RE: Immunohistochemistry TAT In-Reply-To: References: <77DD817201982748BC67D7960F2F76AF0505C7@UWHC-MBX12.uwhis.hosp.wisc.edu> <6D6BD1DE8A5571489398B392A38A71579D28918A@xmdb04.nch.kids> Message-ID: <6D6BD1DE8A5571489398B392A38A71579D289604@xmdb04.nch.kids> Good as long as the wax has a chance to roll off the slide. We tend to use a drying oven. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Karlisch, Patricia [mailto:pkarlisch@hmc.psu.edu] Sent: Wednesday, 3 July 2013 11:35 PM To: Tony Henwood (SCHN); 'Sebree Linda A'; 'histonet@lists.utsouthwestern.edu' Subject: RE: Immunohistochemistry TAT Tony, Great information! We will consider keeping the dewaxing time in mind. What do you think of hotplate warming? Pat -----Original Message----- From: Tony Henwood (SCHN) [mailto:tony.henwood@health.nsw.gov.au] Sent: Tuesday, July 02, 2013 7:08 PM To: 'Sebree Linda A'; Karlisch, Patricia; 'histonet@lists.utsouthwestern.edu' Subject: RE: Immunohistochemistry TAT Yes, I agree with most. But remember a major part of dewaxing sections is to heat the sections to remove as much wax as possible. This allows the xylene or hydrocarbon dewaxing to be as efficient as it can. Remember to be wary of drying temperature, too high and some antigens (eg S100, 5D3, CMV) will be adversely affected (see Henwood, A., (2005) "Effect of Slide Drying at 80?C on Immunohistochemistry" J Histotechnol 28(1):45-46). We have noticed that staining for S100 was weaker on sections that had not been heated for as long as our routine time (30minutes 64oC). One wonders whether heated detergent dewaxing might be more efficient at removing wax (see Henwood, A. F., Prasad, L., & Bourke, V. M. (2013). "The application of heated detergent dewaxing and rehydration to techniques for the demonstration of fungi: a comparison to routine xylene-alcohol dewaxing" J Histotechnol 36(2):45-50) It is also possible that different waxes (especially in older, "cured" blocks) have different de-waxing requirements. Something to consider! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A Sent: Tuesday, 2 July 2013 10:42 PM To: 'Karlisch, Patricia'; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Immunohistochemistry TAT Pat, Right off the bat I can see 2 things that could speed things up for you. We have Ultras and only dry our slides for 10 minutes at 60 d C. If tissues don't stay on well, try some "Stay On" in your water bath; we're experimenting with that now. Also, we spend way too much time ourselves searching for blocks. See if there is a way to have them organized immediately after sectioning the H&Es so they are quicker to find...we are struggling with this. My 2 cents; good luck. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Karlisch, Patricia Sent: Monday, July 01, 2013 4:36 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Immunohistochemistry TAT Histonetters, I am looking for some help in getting IHC slides out in an 8 hour period of time. Here is some information. * We have a busy Immunohistochemistry lab and the volume of requests keep increasing. We offer approximately 150 different IHC stains ( including ISH and Dual ISH for Her 2). Generally, there are request for >150- 200 IHC tests twice a day, not including negative and positive (same slide) controls. * The techs pull an AM log at 6am and another at 1pm. The 1pm log will be handled while waiting for the IHC's to be completed from the 6am run and these will always be run overnight. * The 6am log requires that one tech start cutting each block for multiple IHC stains. (A search for blocks and the necessary control slide occurs by the same person). The slides are kept in a 60 degree oven for about 60 minutes. Most of these slides will be ready by 2-3:30pm. Here is my question. If we allowed requests for IHC stains to wait until 9AM how could we still get slides out by 3:30pm-4pm? In other words we would not have access to an LIS log until 9am so that the pathologists could order from the morning biopsies. What is the best way to do this with 3 Ventana Ultra's and one Benchmark XT. The blocks ( usually 40-50) need to be cut; sit in an oven and then get labeled and placed on the Ventana's. All longer stains such as ISH would be run overnight. The following tasks makes the 3:30- 4pm deadline difficult: * Searching for the blocks * Cutting the blocks (40-50) onto control slides + negative control * One hour in the oven to dry (Can we use 30minutes?) * Attaching labels to 150 slides and setting up instruments * One microtome * 2 techs/ some of the time Can you share your workflow with me if you do a similar volume of slides within an 8 hours period. How would you staff the two IHC techs to gain the most efficiency. As you know the fuller the instrument the longer the staining process. Thank you, Pat Karlisch, Supervisor pkarlisch@hmc.psu.edu Tel 717-531-6072 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From Tony_Reilly <@t> health.qld.gov.au Wed Jul 3 20:48:01 2013 From: Tony_Reilly <@t> health.qld.gov.au (Tony Reilly) Date: Wed Jul 3 20:48:39 2013 Subject: [Histonet] Re: H&E staining question In-Reply-To: References: <9F3CFEE76E51B64991C7485270890B404978C64C@EX4.lj.gnf.org>,, <1217DDB3D7DE5E418E3D560A268EABD048E0ECA0@xmdb03.nch.kids>, <5F46D504-1CC1-419E-BFFE-C928C02AB642@gnf.org>, <51D53B01.411C.0039.0@health.qld.gov.au>, <1372895659.60631.YahooMailNeo@web140603.mail.bf1.yahoo.com>, <1372896385.57994.YahooMailNeo@web140601.mail.bf1.yahoo.com> Message-ID: <51D560F1.411C.0039.0@health.qld.gov.au> The lab I work in now did GMA for all Bone marrows as well, of course with B5 fixation. Some of the haematologists still miss the old days. In those days everything revolved around quality of work. Now we have to balance practicality, cost, OH&S and time to get a compromised method. regards Tony >>> Patsy Ruegg 7/4/2013 10:36 am >>> I did a lot of GMA work on bone marrows and lymph nodes back in the late 70's and early 80's, we had a special H&E developed by my hematopathologist that was Hematoxylin (Harris before Gills came out) eosin and azure we called it HEA I just ran across the protocol a few days ago i should post it for old time sake, it was brilliant for 2 micron sections of hematopoetic tissue, hematologist are the best morphologist in my opinion and demanded thin high quality histology sections. The nuclear detail with HEA is phenomenal. A little off topic we did not use an oxidizer. > Date: Wed, 3 Jul 2013 17:06:25 -0700 > From: akemiat3377@yahoo.com > To: akemiat3377@yahoo.com; Tony_Reilly@health.qld.gov.au; TJohnson@gnf.org; linda.prasad@health.nsw.gov.au > Subject: Re: [Histonet] Re: H&E staining question > CC: histonet@lists.utsouthwestern.edu > > Oh by the way, the reason I did a Harris hematoxylin and eosin/phloxine on GMA (plastics) was because our pathologists at OHSU hated Gills and were used to seeing Harris hematoxylin on all of our regular surgical and autopsy slides. We only did GMA on kidney, liver, some GI and BM core bx's. > > I was just a plain ole histologist that wanted to please her pathologists! Anything can be accomplished if you are creative and innovative! > > Akemi Allison BS, HT(ASCP)HTL > Director > Phoenix Lab Consulting > E-Mail: akemiat3377@yahoo.com > > > > > ________________________________ > From: Akemi Allison > To: Tony Reilly ; Teri Johnson ; Linda Prasad (SCHN) > Cc: "histonet@lists.utsouthwestern.edu" > Sent: Wednesday, July 3, 2013 4:54 PM > Subject: Re: [Histonet] Re: H&E staining question > > > Tony: > I actually was going to suggest this. You beat me to it! I used to do GMA on 1.5 microns sections and did a Harris hematoxylin and eosin/phloxine routinely (by the way, this was not recommended back in 1980. Gills hematoxylin was the norm!). > > To get the results I wanted, I would routinely oxidize the slides in 1% periodic acid and rince in running tap water before staining in hematoxylin. the staining was intensified and was beautiful! I came up with this because my PAS's with hematoxylin counterstains looked better than routine H&E's. I figuired it was worth doing the GMA's routinely using 1% periodic acid since we didn't do that many. Funny how we stumble on our modifications! > > Science never sleeps!! > > Akemi Allison BS, HT(ASCP)HTL > Director > Phoenix Lab Consulting > E-Mail: akemiat3377@yahoo.com > > > > > ________________________________ > From: Tony Reilly > To: Teri Johnson ; Linda Prasad (SCHN) > Cc: "histonet@lists.utsouthwestern.edu" > Sent: Wednesday, July 3, 2013 4:06 PM > Subject: [Histonet] Re: H&E staining question > > > Hi Linda > > For a little more information (not an article) go to: > http://www.ihcworld.com/royellis/problems/problem21.htm > > regards > Tony > > > > > Tony Reilly B.App.Sc. , M.Sc. > Chief Scientist, Anatomical Pathology > Pathology Queensland-PA Laboratory > ________________________________________________ > Health Services Support Agency | Department of Health > > Level 1, Building 15,Princess Alexandra Hospital > Ipswich Road,WOOLLOONGABBA Qld4102 > Ph: 07 3176 2412 > Mob: 0402 139411 > Fax: 07 3176 2930 > Email: tony_reilly@health.qld.gov.au > Web: www.health.qld.gov.au/qhcss/ > > > > > >>> Teri Johnson 7/4/2013 12:37 am >>> > Hi Linda, > > There is no journal article, merely an observation on my part many years ago. > > Teri > > On Jul 2, 2013, at 10:05 PM, "Linda Prasad (SCHN)" wrote: > > > Hi Teri, > > Oxidising the slides in periodic acid prior to H&E improves the HE. Would really appreciate if you could please send me the reference for that article. Thank you Teri. > > > > > > Linda Prasad, MSc, BSc > > > > Hospital Scientist | Histopathology > > t: 02 9845 3316 | f: 02 9845 3318 | e: linda.prasad@health.nsw.gov.au | w: www.schn.health.nsw.gov.au > > m: 0425 314 267 > > > > > > > > Cnr Hawkesbury Road and Hainsworth Street, Westmead > > Locked Bag 4001, Westmead NSW 2145 > > > > P Please consider the environment before printing this email. > > > > ________________________________________ > > From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Teri Johnson [TJohnson@gnf.org] > > Sent: Wednesday, 3 July 2013 3:24 AM > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] Re: H&E staining question > > > > Hi Brett, > > > > I agree with the others, the storage in 70% ETOH is likely not the cause of the washed out appearance of the staining. > > > > * Did the lab that did the H&E staining run a daily control? How did that look? Perhaps the tap water they are using went a little funny? Perhaps the depar xylene needs refreshing? The hematoxylin is used up? If all the other H&Es done that day look good, then look further. > > > > * Look at the fixative, it is possible to get bad batches of that. It is also possible to cram a lot of tissue into a small space resulting in inadequate fixation. Or perhaps it was bloody and not changed to fresh fixative? Was one sample fixed at room temperature and the other fixed at 4 degrees C? Was the person doing the necropsy the same among animal batches? > > > > * Two things might help get better hematoxylin staining - I recall a journal article about using antigen retrieval pretreatment to improve H&E staining on samples that had been stored in fixative for a prolonged period of time. That might help. Years ago, I also noticed that the hematoxylin counterstained PAS slides looked better than our H&Es, so we put a bucket of 0.5% periodic acid as an oxidation step before hematoxylin. It needs to be rinsed well so there is no periodic acid carryover (that'll kill your hematoxylin!), but that might improve your staining. > > > > If you get this figured out, please let us know the cause and fix. > > > > Teri Johnson > > Manager, Histology > > GNF - San Diego, CA > > 858-332-4752 > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ********************************************************************************* > > This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. > > > > Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. > > > > This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. > > ********************************************************************************* > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ******************************************************************************** > This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. > Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. > If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. > If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. > Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. > Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. > ********************************************************************************** > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From ewj <@t> pigsqq.org Fri Jul 5 08:27:54 2013 From: ewj <@t> pigsqq.org (ewj@pigsqq.org) Date: Fri Jul 5 08:28:05 2013 Subject: [Histonet] periodic acid/h&e Message-ID: <20130705132754.12098.qmail@station195.com> Thanks for the tip. I had some periodic acid so I tried a 2 minute soak in a 0.5% soln of that followed by a tap water wash and then dw then Harris hematoxylin countered with a Eosin-Biebrich scarlet we like on some classical swine fever slides I looked at today. Very nice. I will play with this more. By the way, We use the hot water/detergent method for all our dewaxing now. We still are using xylene and alcohols for our processing from fixative to paraffin but we will change that eventually. E. Wayne Johnson Enable Ag Tech Beijing From ewj <@t> pigsqq.org Fri Jul 5 08:27:54 2013 From: ewj <@t> pigsqq.org (ewj@pigsqq.org) Date: Fri Jul 5 08:28:06 2013 Subject: [Histonet] periodic acid/h&e Message-ID: <20130705132754.12098.qmail@station195.com> Thanks for the tip. I had some periodic acid so I tried a 2 minute soak in a 0.5% soln of that followed by a tap water wash and then dw then Harris hematoxylin countered with a Eosin-Biebrich scarlet we like on some classical swine fever slides I looked at today. Very nice. I will play with this more. By the way, We use the hot water/detergent method for all our dewaxing now. We still are using xylene and alcohols for our processing from fixative to paraffin but we will change that eventually. E. Wayne Johnson Enable Ag Tech Beijing From joewalker <@t> rrmc.org Fri Jul 5 08:58:06 2013 From: joewalker <@t> rrmc.org (Joe W. Walker, Jr.) Date: Fri Jul 5 08:58:13 2013 Subject: [Histonet] RE: Cytology Staining In-Reply-To: <7EAFE982E328304DA6CE2B677BB76246794E4337@TN001WEXMBX12.US.chs.net> References: <02AE2390303AAB43A823930EAD6B63AE668A86B9@ex07> <7EAFE982E328304DA6CE2B677BB76246794E4337@TN001WEXMBX12.US.chs.net> Message-ID: <3C2378778400AD448ADA6FD6BDB7CCCC17FB1638@RRMBX03.rrmc.local> So, I'm late to this response, but there are several ways you can avoid floaters. We utilize the ThinPrep process for all body fluids, bronch washes, urines or other fluids we receive. The nature of this type of prep is considered a method for reducing the potential for floaters. You could also use a cytospin and the cytospin collection fluid contains PEG, which helps to the cells adhere to the slide. We generally triage our body cavity fluids by taking a drop (after the material has been placed into a Preservcyt vial) and make a quick wet prep. We add a few drops of toluedine blue to the drop, cover slip and do a rapid review for any positive appearing cells. If found, we stain the body cavity fluid separately. If not, it is batched with the other non-gyn specimens and stained. We do separate out our direct smear prepped FNA's though as they have more of a tendency to shed material when it was not spread correctly, which resulted in thick areas. We generally know if the FNA is positive or not because we attend most of those procedures and provide a rapid interpretation. All stains are filtered if a positive case is encountered. Hope this helps to the conversation, Joe W. Walker, Jr. MS, SCT(ASCP)CM Anatomical Pathology Manager Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P: 802.747.1790 F: 802.747.6525 NEW EMAIL: joewalker@rrmc.org http://www.rrmc.org/ Our Vision: To be the Best Community Healthcare System in New England Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet Recognition? and the Governor's Award for Performance Excellence -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Boyd, Debbie M Sent: Thursday, June 27, 2013 10:13 AM To: McCabe, Sara; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Cytology Staining Body fluids are stained separately as they have a high potential for floating. FNA's , bronchs, thyroids, urine ect. Can all be stained together. After a malignant case is reported all stains are filtered and the first alcohol before each stain is discarded. The first alcohol after each stain is discarded and the others rotated up with a fresh one at the end. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of McCabe, Sara Sent: Wednesday, June 26, 2013 3:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cytology Staining I have a question for those of you that also may perform cytology specimens at your facility. How do you prevent carryover from one case to another? For example, we had obvious carryover from a pleural fluid onto an FNA of a thyroid case. This has never happened here (to our knowledge). Has anyone else every experienced this? We spray fixed our slides with an alcohol/acetone based fixative before staining them. Any advice would be appreciated! Sara McCabe, HT(ASCP) DuBois Regional Medical Center This email and any attached files are confidential and intended solely for the intended recipient(s). If you are not the named recipient you should not read, distribute, copy or alter this email. Any views or opinions expressed in this email are those of the author and do not represent those of the company. Warning: Although precautions have been taken to make sure no viruses are present in this email, the company cannot accept responsibility for any loss or damage that arise from the use of this email or attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. 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If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. Thank You From akemiat3377 <@t> yahoo.com Fri Jul 5 09:38:32 2013 From: akemiat3377 <@t> yahoo.com (Akemi Allison) Date: Fri Jul 5 09:38:36 2013 Subject: [Histonet] periodic acid/h&e In-Reply-To: <20130705132754.12098.qmail@station195.com> References: <20130705132754.12098.qmail@station195.com> Message-ID: <1373035112.63357.YahooMailNeo@web140603.mail.bf1.yahoo.com> I forgot to mention the timing. ?You may try 5 minutes or even 10 minutes before staining in hematoxylin. It will give you even better results. I used 5 minutes in 1?% periodic acid. ? Akemi Allison BS, HT(ASCP)HTL Director Phoenix Lab Consulting E-Mail: akemiat3377@yahoo.com ________________________________ From: "ewj@pigsqq.org" To: histonet@lists.utsouthwestern.edu; """ Sent: Friday, July 5, 2013 6:27 AM Subject: [Histonet] periodic acid/h&e Thanks for the tip.? I had some periodic acid so I tried a 2 minute soak in a 0.5% soln of that followed by a tap water wash and then dw then Harris hematoxylin countered with a Eosin-Biebrich scarlet we like on some classical swine fever slides I looked at today. Very nice.? I will play with this more. By the way, We use the hot water/detergent method for all our dewaxing now. We still are using xylene and alcohols for our processing from fixative to paraffin but we will change that eventually. E. Wayne Johnson Enable Ag Tech Beijing _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kgrobert <@t> rci.rutgers.edu Fri Jul 5 10:17:15 2013 From: kgrobert <@t> rci.rutgers.edu (kgrobert@rci.rutgers.edu) Date: Fri Jul 5 10:17:19 2013 Subject: [Histonet] Processing Drosophila brains into paraffin Message-ID: I was given a protocol for processing Drosophila brains into paraffin manually, and it involves methyl benzoate. It is as follows: Fixation: 3.5-4 hours in Carnoy's fixative, in hood (room temperature) Processing: 2x30 min 99% ethanol (Would 95% be okay?) 60 min in absolute ethanol Overnight in methyl benzoat (that's how it's written in the protocol), can be up to 3 days (*Here is my question-I looked on Sigma's website for this, and found a bunch of similar chemicals, but no straight methyl benzoat. What do I order?) 1 hour methylbenzoat-paraffin 1:1 mix at 60 degrees C 6x20 min 60 degrees C paraffin (we use Paraplast X-tra) Thanks so much! Kathleen Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology & Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (848) 445-1443 FAX (732) 445-6905 From dmlaud <@t> gmail.com Fri Jul 5 11:13:21 2013 From: dmlaud <@t> gmail.com (Damien) Date: Fri Jul 5 11:13:24 2013 Subject: [Histonet] Re: Processing Drosophila into paraffin Message-ID: Hi Kathleen, Yes,95% ethanol is fine. It's (Methyl Benzoate) most likely misspelled in your protocol. Try Sigma catalog # M29903. -Damien L -- Damien Laudier Laudier Histology 917-836-7573 www.LaudierHistology.com * * From ewj <@t> pigsqq.org Fri Jul 5 11:39:25 2013 From: ewj <@t> pigsqq.org (ewj@pigsqq.org) Date: Fri Jul 5 11:39:34 2013 Subject: =?utf-8?B?UmU6IFtIaXN0b25ldF0gcGVyaW9kaWMgYWNpZC9oJmU=?= In-Reply-To: <1373035112.63357.YahooMailNeo@web140603.mail.bf1.yahoo.com> References: <20130705132754.12098.qmail@station195.com> <1373035112.63357.YahooMailNeo@web140603.mail.bf1.yahoo.com> Message-ID: <20130705163925.7810.qmail@station195.com> I have an ancient copy of Humason and she had methods 2 to 5 minutes for PAS etc with Periodic Acid so I tried 2 min today. Will try more time and stronger solutions and will also get around to doing some staining with PAS that I wanted to do to look at goblet cells and compare them to the vacuolated cells produced by porcine epidemic diarrhea... > -------Original Message------- > From: Akemi Allison > To: ewj@pigsqq.org , histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] periodic acid/h&e > Sent: Jul 05 '13 22:38 > > I forgot to mention the timing. ?You may try 5 minutes or even 10 minutes before staining in hematoxylin. It will give you even better results. I used 5 minutes in 1?% periodic acid. > ? > Akemi Allison BS, HT(ASCP)HTL > Director > Phoenix Lab Consulting > E-Mail: akemiat3377@yahoo.com > > > > > ________________________________ > From: "ewj@pigsqq.org" > To: histonet@lists.utsouthwestern.edu; """ > Sent: Friday, July 5, 2013 6:27 AM > Subject: [Histonet] periodic acid/h&e > > > Thanks for the tip.? I had some periodic acid so I tried a 2 minute soak in a 0.5% soln of that followed by a tap water wash and then dw then Harris hematoxylin countered with a Eosin-Biebrich scarlet we like on some classical swine fever slides I looked at today. Very nice.? I will play with this more. > > By the way, We use the hot water/detergent method for all our dewaxing now. > > We still are using xylene and alcohols for our processing from fixative to paraffin but > we will change that eventually. > > E. Wayne Johnson > Enable Ag Tech > Beijing > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From BGapinski <@t> pathgroup.com Fri Jul 5 11:57:29 2013 From: BGapinski <@t> pathgroup.com (Bruce Gapinski) Date: Fri Jul 5 11:57:36 2013 Subject: [Histonet] phobic slides Message-ID: Histonians! Has anyone else had incorrect IHC staining due to overly charged slides that are hydrophobic? My Ventana service department was out because I kept complaining about incomplete staining. For instance, the control at the top of the slide stained with both DAB and hematoxylin. But on the very same slide the hematoxylin was missing from the patient tissue. I told them how this unnerved me. If the same thing happens but the hematoxylin stains and the DAB does not.......I could be looking at a false negative. This may have happened already as far as I know. The solution according to Ventana is to take 4 slides from each new box of slides (1/2 gross) and do a vortex mix on each of them one at a time. Then you know you have valid slides to stain patient tissues! I have no time for this. And I'm trying to believe it but it's just not scientific. How is it that my results have been free of this "phobia" for over a year? Why didn't I get this (EVER) with Dako instrumentation? The answer, I was told, was that the humidity was a problem. If I were to take this to it's ridiculous conclusion, I would need to inform oncologists not to operate during these incorrectly humidified days. It IS summer so I'm not sure how much humidity is required. 1. What brand of slides do you use? 2. Have you had the "slide phobia" so that it compromised the results? (Slide-phobia is for retired Histologists, no?) 3. (I think I know the answer but I just have to ask) Do you validate slides? Bruce Gapinsk HT (ASCP) Chief Histologist Marin Medical Laboratories PathGroup SF ________________________________ Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you From Timothy.Morken <@t> ucsfmedctr.org Fri Jul 5 12:15:04 2013 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Fri Jul 5 12:15:18 2013 Subject: [Histonet] RE: phobic slides In-Reply-To: References: Message-ID: <761E2B5697F795489C8710BCC72141FF085E14@ex07.net.ucsf.edu> I've seen that when using the Dako stainer or manual staining. The small amount of liquid used for the antibody and detection pools up on the slide rather than spreading out. We solved it for those uses with longer soaks in TBS-tween before putting the slides on the stainer and ensuring the rinse buffer and even diluent had tween in it to promote spreading. I wouldn't think that would happen on the Ventana or Bond instruments with their liquid coverslips and coverplates. And you couldn't use the same solution anyway... Tim Morken UCSF Pathology -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bruce Gapinski Sent: Friday, July 05, 2013 9:57 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] phobic slides Histonians! Has anyone else had incorrect IHC staining due to overly charged slides that are hydrophobic? My Ventana service department was out because I kept complaining about incomplete staining. For instance, the control at the top of the slide stained with both DAB and hematoxylin. But on the very same slide the hematoxylin was missing from the patient tissue. I told them how this unnerved me. If the same thing happens but the hematoxylin stains and the DAB does not.......I could be looking at a false negative. This may have happened already as far as I know. The solution according to Ventana is to take 4 slides from each new box of slides (1/2 gross) and do a vortex mix on each of them one at a time. Then you know you have valid slides to stain patient tissues! I have no time for this. And I'm trying to believe it but it's just not scientific. How is it that my results have been free of this "phobia" for over a year? Why didn't I get this (EVER) with Dako instrumentation? The answer, I was told, was that the humidity was a problem. If I were to take this to it's ridiculous conclusion, I would need to inform oncologists not to operate during these incorrectly humidified days. It IS summer so I'm not sure how much humidity is required. 1. What brand of slides do you use? 2. Have you had the "slide phobia" so that it compromised the results? (Slide-phobia is for retired Histologists, no?) 3. (I think I know the answer but I just have to ask) Do you validate slides? Bruce Gapinsk HT (ASCP) Chief Histologist Marin Medical Laboratories PathGroup SF ________________________________ Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wbenton <@t> cua.md Fri Jul 5 12:17:30 2013 From: wbenton <@t> cua.md (Walter Benton) Date: Fri Jul 5 12:17:37 2013 Subject: [Histonet] RE: phobic slides In-Reply-To: References: Message-ID: <0B8979A204680A42B93A52B486088CD93698C8C1A7@CUAEXH1.GCU-MD.local> Bruce, Are you using the slides with the patient and control all in one. If so, is the paint on the top of the slide or the reverse underside? There has been a known problem for years that correlates to what you are describing with the control box slides with paint on the top. Secondly, have you noticed this phenomenon on one instrument or all? If it is one instrument I would check to make sure the vortex mixers are working properly. This is done easily with a drop of Hematoxylin on the slide and turning the vortex mixers on within the instrument. Another possibility could be that your instrument may not be lining up with the holes where the slide positions are located. Not knowing which instrument you are using it is hard to say. The benchmark had both slide carousel and dispenser reagent rack moving which often caused misalignment and solutions would not reach the slide, bit instead would be on the drip tray. The XT can have the same problem, but not nearly as often since only the reagents move and not the slides. As I'm sure you aware slide variability does exist within the pack of slides at times, (I have witnessed this personally with Asian slides specifically, never German or American). It truly depends on the technique being used to charge the slide. Finally, you may not have seen this with Dako instrumentation since it uses a different mechanism to place reagent on the slide. It uses the drop zones and surfactant to have the solution cover the slide. I would imagine that it is less likely to occur on the Dako unless your slides are truly hydrophobic. Hope this helps! Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) ChesapeakeUrology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bruce Gapinski [BGapinski@pathgroup.com] Sent: Friday, July 05, 2013 12:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] phobic slides Histonians! Has anyone else had incorrect IHC staining due to overly charged slides that are hydrophobic? My Ventana service department was out because I kept complaining about incomplete staining. For instance, the control at the top of the slide stained with both DAB and hematoxylin. But on the very same slide the hematoxylin was missing from the patient tissue. I told them how this unnerved me. If the same thing happens but the hematoxylin stains and the DAB does not.......I could be looking at a false negative. This may have happened already as far as I know. The solution according to Ventana is to take 4 slides from each new box of slides (1/2 gross) and do a vortex mix on each of them one at a time. Then you know you have valid slides to stain patient tissues! I have no time for this. And I'm trying to believe it but it's just not scientific. How is it that my results have been free of this "phobia" for over a year? Why didn't I get this (EVER) with Dako instrumentation? The answer, I was told, was that the humidity was a problem. If I were to take this to it's ridiculous conclusion, I would need to inform oncologists not to operate during these incorrectly humidified days. It IS summer so I'm not sure how much humidity is required. 1. What brand of slides do you use? 2. Have you had the "slide phobia" so that it compromised the results? (Slide-phobia is for retired Histologists, no?) 3. (I think I know the answer but I just have to ask) Do you validate slides? Bruce Gapinsk HT (ASCP) Chief Histologist Marin Medical Laboratories PathGroup SF ________________________________ Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From micropathlabs <@t> yahoo.com Fri Jul 5 12:22:07 2013 From: micropathlabs <@t> yahoo.com (Sheila Haas) Date: Fri Jul 5 12:22:11 2013 Subject: [Histonet] phobic slides In-Reply-To: References: Message-ID: <1373044927.51402.YahooMailNeo@web122006.mail.ne1.yahoo.com> I've used Ventana for years and have never had that problem. We use Superfrost Plus slides from Cardinal. I can certainly see that their resolution would be too time consuming and I too would be concerned that the staining would be false if any reagent in the detection kit did not spread properly over the entire slide. I suggest you press it a little further with Ventana. I think there could be other instrumentation?issues that would cause your problem. Hope this helps. Sheila Haas Laboratory Manager MicroPath Laboratories, Inc. ________________________________ From: Bruce Gapinski To: "histonet@lists.utsouthwestern.edu" Sent: Friday, July 5, 2013 12:57 PM Subject: [Histonet] phobic slides Histonians! ? ? ? ? ? ? ? ? Has anyone else had incorrect? IHC? staining due to overly charged slides that are hydrophobic? My Ventana service department was out because I kept complaining about incomplete staining. For instance, the control at the top of the slide stained with? both DAB and hematoxylin. But on the very same slide the hematoxylin was missing from the patient tissue. ? ? ? ? ? ? ? ? I told them how this unnerved me. If the same thing happens but the hematoxylin stains and the DAB does not.......I could be looking at a false negative. This may have happened already as far as I know. ? ? ? ? ? ? ? ? The solution according to Ventana is to take 4 slides from each new box of slides (1/2 gross) and do a vortex mix on each of them one at a time. Then you know you have valid slides to stain patient tissues! I have no time for this. And I'm trying to believe it but it's just not scientific. How is it that my results have been free of this "phobia" for over a year? Why didn't I get this (EVER) with Dako instrumentation? The answer, I was told, was that the humidity was a problem. If I were to take this to it's ridiculous conclusion, I would need to inform oncologists not to operate during these incorrectly humidified days. It IS summer so I'm not sure how much humidity is required. 1.? ? ? What brand of slides do you use? 2.? ? ? Have you had the "slide phobia" so that it compromised the results? (Slide-phobia is for retired Histologists, no?) 3.? ? ? (I think I know the answer but I just have to ask) Do you validate slides? Bruce Gapinsk HT (ASCP) Chief Histologist Marin Medical Laboratories PathGroup SF ________________________________ Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CThornton <@t> dahlchase.com Fri Jul 5 12:32:06 2013 From: CThornton <@t> dahlchase.com (Clare Thornton) Date: Fri Jul 5 12:32:10 2013 Subject: [Histonet] RE: phobic slides In-Reply-To: References: Message-ID: Bruce, We are very familiar with this problem. Yes, we have encountered slides that are hydrophobic, and the reagents don't mix well on the slide. I have photos of this happening in progress! We think it came down to humidity, it always seems to happen in the summer months. We use Superfrost plus slides. Needless to say, when we enounter this problem we end up doing a lot of repeats. If you can, try a different lot of slides, sometimes this helps. I'll be glad to talk to you more about it offline if you wish. Clare J. Thornton, HTL(ASCP),QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bruce Gapinski Sent: Friday, July 05, 2013 12:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] phobic slides Histonians! Has anyone else had incorrect IHC staining due to overly charged slides that are hydrophobic? My Ventana service department was out because I kept complaining about incomplete staining. For instance, the control at the top of the slide stained with both DAB and hematoxylin. But on the very same slide the hematoxylin was missing from the patient tissue. I told them how this unnerved me. If the same thing happens but the hematoxylin stains and the DAB does not.......I could be looking at a false negative. This may have happened already as far as I know. The solution according to Ventana is to take 4 slides from each new box of slides (1/2 gross) and do a vortex mix on each of them one at a time. Then you know you have valid slides to stain patient tissues! I have no time for this. And I'm trying to believe it but it's just not scientific. How is it that my results have been free of this "phobia" for over a year? Why didn't I get this (EVER) with Dako instrumentation? The answer, I was told, was that the humidity was a problem. If I were to take this to it's ridiculous conclusion, I would need to inform oncologists not to operate during these incorrectly humidified days. It IS summer so I'm not sure how much humidity is required. 1. What brand of slides do you use? 2. Have you had the "slide phobia" so that it compromised the results? (Slide-phobia is for retired Histologists, no?) 3. (I think I know the answer but I just have to ask) Do you validate slides? Bruce Gapinsk HT (ASCP) Chief Histologist Marin Medical Laboratories PathGroup SF ________________________________ Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jshea121 <@t> roadrunner.com Sat Jul 6 13:17:09 2013 From: jshea121 <@t> roadrunner.com (Shea's) Date: Sat Jul 6 13:17:18 2013 Subject: [Histonet] phobic slides Message-ID: <635BD53F70CA461C84044787BC280925@JoannePC> We too have experienced problems (uneven H&E staining) from such slides. We were using Super-frost Plus without a problem, however, thought we could save a few dollars with another vendor. We noticed their charged slides worked too well in that they trapped water under the slide after picking up the section from the water bath. After notifying the vendor, we were instructed to pick up the sections more vertically. Really? Only been doing this for 30 years. Even our usual procedure of a gentle tap of the slide to release the extra water, air drying for 20 minutes, then drying in a 60 degree slide drying oven for 1 hour, still showed some water trapped at the bottom of the section. As a result, we stayed with what worked. Jan From abijag76 <@t> rediffmail.com Sun Jul 7 08:25:31 2013 From: abijag76 <@t> rediffmail.com (abijag ) Date: Sun Jul 7 08:25:42 2013 Subject: [Histonet] collagen I IHC detection in mouse pulmonary fibrosis Message-ID: <20130707132531.23041.qmail@f4mail-235-219.rediffmail.com> All, I would like to quantify collagen 1 in bleomycin induced lung fibrosis in mice. It will be highly appreciated if any of our histonetters will share their protocol for IHC staining of perfusion fixed(10% NBF) mouse fibrotic lungs. Thanks in advance Abi From Tony_Reilly <@t> health.qld.gov.au Sun Jul 7 18:15:54 2013 From: Tony_Reilly <@t> health.qld.gov.au (Tony Reilly) Date: Sun Jul 7 18:16:39 2013 Subject: [Histonet] phobic slides In-Reply-To: References: Message-ID: <51DA8349.411C.0039.0@health.qld.gov.au> Hi Bruce Our Immuno staff have reported the uneven staining artifact with some brands of coated slides but not others and we are staining on the Ventana platform. I have also noticed in some cases when slides are inadequately dewaxed the Haematoxylin will stain even though there is loss of immunostaining.. regards Tony Tony Reilly B.App.Sc. , M.Sc. Chief Scientist, Anatomical Pathology Pathology Queensland-PA Laboratory ________________________________________________ Health Services Support Agency | Department of Health Level 1, Building 15,Princess Alexandra Hospital Ipswich Road,WOOLLOONGABBA Qld4102 Ph: 07 3176 2412 Mob: 0402 139411 Fax: 07 3176 2930 Email: tony_reilly@health.qld.gov.au Web: www.health.qld.gov.au/qhcss/ >>> Bruce Gapinski 7/6/2013 2:57 am >>> Histonians! Has anyone else had incorrect IHC staining due to overly charged slides that are hydrophobic? My Ventana service department was out because I kept complaining about incomplete staining. For instance, the control at the top of the slide stained with both DAB and hematoxylin. But on the very same slide the hematoxylin was missing from the patient tissue. I told them how this unnerved me. If the same thing happens but the hematoxylin stains and the DAB does not.......I could be looking at a false negative. This may have happened already as far as I know. The solution according to Ventana is to take 4 slides from each new box of slides (1/2 gross) and do a vortex mix on each of them one at a time. Then you know you have valid slides to stain patient tissues! I have no time for this. And I'm trying to believe it but it's just not scientific. How is it that my results have been free of this "phobia" for over a year? Why didn't I get this (EVER) with Dako instrumentation? The answer, I was told, was that the humidity was a problem. If I were to take this to it's ridiculous conclusion, I would need to inform oncologists not to operate during these incorrectly humidified days. It IS summer so I'm not sure how much humidity is required. 1. What brand of slides do you use? 2. Have you had the "slide phobia" so that it compromised the results? (Slide-phobia is for retired Histologists, no?) 3. (I think I know the answer but I just have to ask) Do you validate slides? Bruce Gapinsk HT (ASCP) Chief Histologist Marin Medical Laboratories PathGroup SF ________________________________ Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ********************************************************************************** From abtdhu <@t> gmail.com Sun Jul 7 21:05:57 2013 From: abtdhu <@t> gmail.com (Dorothy Hu) Date: Sun Jul 7 21:06:01 2013 Subject: [Histonet] Calcein labeled plastic section mounting procedure Message-ID: Hi All, I am relatively new to plastic embedding media. I need to permanently mount acrylosin calcein labeled undecaled bone sections under coverslip. Would be possible for someone tell me that do I need to de-plastic first in xylene? What mounting media should I use to protect fluorescent green color fading? Much gratitude. Dorothy Hu abtdhu@gmail.com From Melissa.Kuhnla <@t> chsli.org Mon Jul 8 06:39:22 2013 From: Melissa.Kuhnla <@t> chsli.org (Kuhnla, Melissa) Date: Mon Jul 8 06:39:34 2013 Subject: [Histonet] MMR antibodies Message-ID: Good Morning, We are beginning to optimize and validate the four antibodies for colon MMR (MLH1, MSH2, MSH6, PMS2). We are having difficulties gathering cases to show appropriate loss of staining for all four antibodies. Can anyone share ideas on how this can be accomplished? We are already reaching out to our genetics department and reference lab for cases we have sent in the past. Thank you :-) Melissa Kuhnla Lead Medical Technologist for IHC and FISH Catholic Health Services of Long Island Regional Laboratory Services The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. From jshelley <@t> sanfordburnham.org Mon Jul 8 07:47:42 2013 From: jshelley <@t> sanfordburnham.org (John Shelley) Date: Mon Jul 8 07:47:47 2013 Subject: [Histonet] RE: phobic slides In-Reply-To: References: Message-ID: Hi Bruce, I just seen your post and I will tell you that I have seen this problem on a Ventana instrument as well. I mostly see it when slides other than superfrost plus slides are used however I have even seen it on those as well. I was told one time to use an antibody diluent just before placing any antibody on the slides. I understand that you most likely do not open your instrument once it is started but I titrate by hand and I have seen it as early as antibody titration or begins after that at secondary titration. There is no rhyme or reason but I can tell you that the oils on one's hand can cause this as well. Would love to hear about the resolution if there is any. Thanks!!! Kind Regards! ? John J Shelley From relia1 <@t> earthlink.net Mon Jul 8 09:15:48 2013 From: relia1 <@t> earthlink.net (Pam Barker) Date: Mon Jul 8 09:15:51 2013 Subject: [Histonet] Hot Histology Job Alert from Pam Barker at RELIA Solutions!! A RELIA Exclusive!! Message-ID: <000501ce7be5$a5654dd0$f02fe970$@earthlink.net> Hi Histonetters!! I hope everybody had a great 4th of July holiday weekend. I wanted to write a quick post and let you know about a new opportunity that I am working on. This is a RELIA Exclusive!! Here is the information: ASCP HT/HTL Histology Tech - Bristol, TN RELIA Solutions, the nation's only recruiting firm specializing exclusively in the permanent placement of histology techs is working with a brand new client in Bristol, TN that is in need of a day shift ASCP certified histotech. Knowledge of routine histology- cutting, embedding, special stains etc. is required. At least 1 year of experience is preferred. My client offers an excellent compensation and benefits package and a great group of people to work with. They are ready to hire today!! For more information pleases contact Pam Barker at relia1@earthlink.net or toll free at 866-607-3542. Keywords: histology, histologist, histotechnician, histotechnologist. Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From davidclark10 <@t> comcast.net Mon Jul 8 13:10:22 2013 From: davidclark10 <@t> comcast.net (davidclark10@comcast.net) Date: Mon Jul 8 13:20:51 2013 Subject: [Histonet] Plastic/Plexiglass Incubation chamber for IHC slides In-Reply-To: <493436627.1040751.1373306890387.JavaMail.root@sz0051a.emeryville.ca.mail.comcast.net> Message-ID: <722423942.1040847.1373307022705.JavaMail.root@sz0051a.emeryville.ca.mail.comcast.net> Hi Histonetters, I hope you all had a wonderful Independence Day! Do you guys remember in the olden days before automation when we did IHC by hand? I'm looking for an incubation chamber that allows us to incubate our slides overnight. The one I have right now is about 20x6x6 with a lid and allows for about 4 flat racks of slides to be placed inside. Any information would be appreciated either if you have one or know of a company that can make one. Cheers! David Clark Oregon Health & Science University Portland, Oregon From wbenton <@t> cua.md Mon Jul 8 13:20:24 2013 From: wbenton <@t> cua.md (Walter Benton) Date: Mon Jul 8 13:20:59 2013 Subject: [Histonet] Plastic/Plexiglass Incubation chamber for IHC slides In-Reply-To: <722423942.1040847.1373307022705.JavaMail.root@sz0051a.emeryville.ca.mail.comcast.net> References: <493436627.1040751.1373306890387.JavaMail.root@sz0051a.emeryville.ca.mail.comcast.net>, <722423942.1040847.1373307022705.JavaMail.root@sz0051a.emeryville.ca.mail.comcast.net> Message-ID: <0B8979A204680A42B93A52B486088CD938E883CB5D@CUAEXH1.GCU-MD.local> http://www.ihcworld.com/products/IHC-Staining-System.htm I have not used, but it seems that it would work like the old ones. Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) ChesapeakeUrology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of davidclark10@comcast.net [davidclark10@comcast.net] Sent: Monday, July 08, 2013 2:10 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Plastic/Plexiglass Incubation chamber for IHC slides Hi Histonetters, I hope you all had a wonderful Independence Day! Do you guys remember in the olden days before automation when we did IHC by hand? I'm looking for an incubation chamber that allows us to incubate our slides overnight. The one I have right now is about 20x6x6 with a lid and allows for about 4 flat racks of slides to be placed inside. Any information would be appreciated either if you have one or know of a company that can make one. Cheers! David Clark Oregon Health & Science University Portland, Oregon _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From BDeBrosse-Serra <@t> isisph.com Mon Jul 8 13:57:01 2013 From: BDeBrosse-Serra <@t> isisph.com (Bea DeBrosse-Serra) Date: Mon Jul 8 13:57:12 2013 Subject: [Histonet] Plastic/Plexiglass Incubation chamber for IHC slides In-Reply-To: <0B8979A204680A42B93A52B486088CD938E883CB5D@CUAEXH1.GCU-MD.local> References: <493436627.1040751.1373306890387.JavaMail.root@sz0051a.emeryville.ca.mail.comcast.net>, <722423942.1040847.1373307022705.JavaMail.root@sz0051a.emeryville.ca.mail.comcast.net> <0B8979A204680A42B93A52B486088CD938E883CB5D@CUAEXH1.GCU-MD.local> Message-ID: <493CAA64F203E14E8823737B9EE0E25F09488DD8B9@EXCHMB01.isis.local> We do IHC by hand most of the times.............. http://www.newcomersupply.com/products/slide-staining-trays-ihc Bea Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Walter Benton Sent: Monday, July 08, 2013 11:20 AM To: davidclark10@comcast.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Plastic/Plexiglass Incubation chamber for IHC slides http://www.ihcworld.com/products/IHC-Staining-System.htm I have not used, but it seems that it would work like the old ones. Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) ChesapeakeUrology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of davidclark10@comcast.net [davidclark10@comcast.net] Sent: Monday, July 08, 2013 2:10 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Plastic/Plexiglass Incubation chamber for IHC slides Hi Histonetters, I hope you all had a wonderful Independence Day! Do you guys remember in the olden days before automation when we did IHC by hand? I'm looking for an incubation chamber that allows us to incubate our slides overnight. The one I have right now is about 20x6x6 with a lid and allows for about 4 flat racks of slides to be placed inside. Any information would be appreciated either if you have one or know of a company that can make one. Cheers! David Clark Oregon Health & Science University Portland, Oregon _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bheller <@t> cellmarque.com Mon Jul 8 13:57:32 2013 From: bheller <@t> cellmarque.com (Brent Heller) Date: Mon Jul 8 13:58:35 2013 Subject: [Histonet] Plastic/Plexiglass Incubation chamber for IHC slides In-Reply-To: <493CAA64F203E14E8823737B9EE0E25F09488DD8B9@EXCHMB01.isis.local> References: <493436627.1040751.1373306890387.JavaMail.root@sz0051a.emeryville.ca.mail.comcast.net>, <722423942.1040847.1373307022705.JavaMail.root@sz0051a.emeryville.ca.mail.comcast.net> <0B8979A204680A42B93A52B486088CD938E883CB5D@CUAEXH1.GCU-MD.local> <493CAA64F203E14E8823737B9EE0E25F09488DD8B9@EXCHMB01.isis.local> Message-ID: Start heating. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bea DeBrosse-Serra Sent: Monday, July 08, 2013 11:57 AM To: 'Walter Benton'; davidclark10@comcast.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Plastic/Plexiglass Incubation chamber for IHC slides We do IHC by hand most of the times.............. http://www.newcomersupply.com/products/slide-staining-trays-ihc Bea Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Walter Benton Sent: Monday, July 08, 2013 11:20 AM To: davidclark10@comcast.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Plastic/Plexiglass Incubation chamber for IHC slides http://www.ihcworld.com/products/IHC-Staining-System.htm I have not used, but it seems that it would work like the old ones. Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) ChesapeakeUrology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of davidclark10@comcast.net [davidclark10@comcast.net] Sent: Monday, July 08, 2013 2:10 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Plastic/Plexiglass Incubation chamber for IHC slides Hi Histonetters, I hope you all had a wonderful Independence Day! Do you guys remember in the olden days before automation when we did IHC by hand? I'm looking for an incubation chamber that allows us to incubate our slides overnight. The one I have right now is about 20x6x6 with a lid and allows for about 4 flat racks of slides to be placed inside. Any information would be appreciated either if you have one or know of a company that can make one. Cheers! David Clark Oregon Health & Science University Portland, Oregon _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sdysart <@t> mirnarx.com Mon Jul 8 14:00:53 2013 From: sdysart <@t> mirnarx.com (Sarah Dysart) Date: Mon Jul 8 14:01:01 2013 Subject: [Histonet] Plastic/Plexiglass Incubation chamber for IHC slides In-Reply-To: References: <493436627.1040751.1373306890387.JavaMail.root@sz0051a.emeryville.ca.mail.comcast.net>, <722423942.1040847.1373307022705.JavaMail.root@sz0051a.emeryville.ca.mail.comcast.net> <0B8979A204680A42B93A52B486088CD938E883CB5D@CUAEXH1.GCU-MD.local> <493CAA64F203E14E8823737B9EE0E25F09488DD8B9@EXCHMB01.isis.local> Message-ID: <858d14533b8b444ebc6e419d0764d021@BY2PR07MB106.namprd07.prod.outlook.com> It's not the "old days" in research world...I still do all my IHC by hand =) I use a vegetable steamer I bought at Walmart and Coplin jars full of the retrieval solution...just an inexpensive solution if you need one? Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Brent Heller Sent: Monday, July 08, 2013 1:58 PM To: 'Bea DeBrosse-Serra'; 'Walter Benton'; davidclark10@comcast.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Plastic/Plexiglass Incubation chamber for IHC slides Start heating. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bea DeBrosse-Serra Sent: Monday, July 08, 2013 11:57 AM To: 'Walter Benton'; davidclark10@comcast.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Plastic/Plexiglass Incubation chamber for IHC slides We do IHC by hand most of the times.............. http://www.newcomersupply.com/products/slide-staining-trays-ihc Bea Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Walter Benton Sent: Monday, July 08, 2013 11:20 AM To: davidclark10@comcast.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Plastic/Plexiglass Incubation chamber for IHC slides http://www.ihcworld.com/products/IHC-Staining-System.htm I have not used, but it seems that it would work like the old ones. Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) ChesapeakeUrology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of davidclark10@comcast.net [davidclark10@comcast.net] Sent: Monday, July 08, 2013 2:10 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Plastic/Plexiglass Incubation chamber for IHC slides Hi Histonetters, I hope you all had a wonderful Independence Day! Do you guys remember in the olden days before automation when we did IHC by hand? I'm looking for an incubation chamber that allows us to incubate our slides overnight. The one I have right now is about 20x6x6 with a lid and allows for about 4 flat racks of slides to be placed inside. Any information would be appreciated either if you have one or know of a company that can make one. Cheers! David Clark Oregon Health & Science University Portland, Oregon _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From shive003 <@t> umn.edu Mon Jul 8 15:16:01 2013 From: shive003 <@t> umn.edu (Jan Shivers) Date: Mon Jul 8 15:16:04 2013 Subject: [Histonet] Plastic/Plexiglass Incubation chamber for IHC slides In-Reply-To: <722423942.1040847.1373307022705.JavaMail.root@sz0051a.emeryville.ca.mail.comcast.net> References: <493436627.1040751.1373306890387.JavaMail.root@sz0051a.emeryville.ca.mail.comcast.net> <722423942.1040847.1373307022705.JavaMail.root@sz0051a.emeryville.ca.mail.comcast.net> Message-ID: When doing manuals, I use the Newcomer Supply humidity chamber that holds 20 slides, but when I run out of space, I always have my trusty metal cake pan with lid (and 1 ml disposable pipets for slide racks) from the 'old days'. Jan Shivers UMN Vet Diag Lab St. Paul, MN On Mon, Jul 8, 2013 at 1:10 PM, wrote: > Hi Histonetters, > > > I hope you all had a wonderful Independence Day! > > > Do you guys remember in the olden days before automation when we did IHC > by hand? > > > I'm looking for an incubation chamber that allows us to incubate our > slides overnight. The one I have right now is about 20x6x6 with a lid > > > and allows for about 4 flat racks of slides to be placed inside. Any > information would be appreciated either if you have one or know of a > company that can make one. > > > Cheers! > > > David Clark > Oregon Health & Science University > Portland, Oregon > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From amber.mckenzie <@t> gastrodocs.net Mon Jul 8 15:20:42 2013 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Mon Jul 8 15:20:51 2013 Subject: [Histonet] Accessioning specimes In-Reply-To: References: <5BD80891-C417-4913-8A0D-2F40AA47F8DB@gmail.com> Message-ID: <5A33C952BB67F4468AF1F36D739212BCBD6B8788@JERRY.Gia.com> When accessioning, do you label the paperwork AND the container with the surgical number? If so, do you hand write the number or use a label maker? Also, do you keep your embedding logs for 2 years like CAP requires QA and QC records? From Joyce.Weems <@t> emoryhealthcare.org Mon Jul 8 15:26:02 2013 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Mon Jul 8 15:26:12 2013 Subject: [Histonet] RE: Accessioning specimes In-Reply-To: <5A33C952BB67F4468AF1F36D739212BCBD6B8788@JERRY.Gia.com> References: <5BD80891-C417-4913-8A0D-2F40AA47F8DB@gmail.com> <5A33C952BB67F4468AF1F36D739212BCBD6B8788@JERRY.Gia.com> Message-ID: We label both - with computer generated labels. Yes, we treat the embedding logs as QC and keep them the required 2 years. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Monday, July 08, 2013 4:21 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Accessioning specimes When accessioning, do you label the paperwork AND the container with the surgical number? If so, do you hand write the number or use a label maker? Also, do you keep your embedding logs for 2 years like CAP requires QA and QC records? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From lblazek <@t> digestivespecialists.com Mon Jul 8 15:35:26 2013 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Mon Jul 8 15:35:30 2013 Subject: [Histonet] RE: Accessioning specimes In-Reply-To: <5A33C952BB67F4468AF1F36D739212BCBD6B8788@JERRY.Gia.com> References: <5BD80891-C417-4913-8A0D-2F40AA47F8DB@gmail.com> <5A33C952BB67F4468AF1F36D739212BCBD6B8788@JERRY.Gia.com> Message-ID: <5A2BD13465E061429D6455C8D6B40E391656C21638@IBMB7Exchange.digestivespecialists.com> We label both the paperwork and the container with the surgical number. We get Avery labels that are four across and have a program set up to print batches of consecutive numbers four across. We keep our embedding logs in the computer. Linda Blazek HT (ASCP) Manager/Supervisor Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Monday, July 08, 2013 4:21 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Accessioning specimes When accessioning, do you label the paperwork AND the container with the surgical number? If so, do you hand write the number or use a label maker? Also, do you keep your embedding logs for 2 years like CAP requires QA and QC records? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Mon Jul 8 15:36:14 2013 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Mon Jul 8 15:36:21 2013 Subject: [Histonet] RE: Accessioning specimes In-Reply-To: References: <5BD80891-C417-4913-8A0D-2F40AA47F8DB@gmail.com>, , <5A33C952BB67F4468AF1F36D739212BCBD6B8788@JERRY.Gia.com>, Message-ID: Yes, ( all associated items),bar code ( machine read) and written(people read),yes, cross check each, yes, keep all logs a minimum of 2 years Joelle Weaver MAOM, HTL (ASCP) QIHC > From: Joyce.Weems@emoryhealthcare.org > To: amber.mckenzie@gastrodocs.net; histonet@lists.utsouthwestern.edu > Date: Mon, 8 Jul 2013 20:26:02 +0000 > CC: > Subject: [Histonet] RE: Accessioning specimes > > We label both - with computer generated labels. > > Yes, we treat the embedding logs as QC and keep them the required 2 years. > > Joyce Weems > Pathology Manager > 678-843-7376 Phone > 678-843-7831 Fax > joyce.weems@emoryhealthcare.org > > > > www.saintjosephsatlanta.org > 5665 Peachtree Dunwoody Road > Atlanta, GA 30342 > > This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie > Sent: Monday, July 08, 2013 4:21 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Accessioning specimes > > > When accessioning, do you label the paperwork AND the container with the surgical number? If so, do you hand write the number or use a label maker? > > Also, do you keep your embedding logs for 2 years like CAP requires QA and QC records? > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ________________________________ > > This e-mail message (including any attachments) is for the sole use of > the intended recipient(s) and may contain confidential and privileged > information. If the reader of this message is not the intended > recipient, you are hereby notified that any dissemination, distribution > or copying of this message (including any attachments) is strictly > prohibited. > > If you have received this message in error, please contact > the sender by reply e-mail message and destroy all copies of the > original message (including attachments). > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Tony_Reilly <@t> health.qld.gov.au Mon Jul 8 21:47:09 2013 From: Tony_Reilly <@t> health.qld.gov.au (Tony Reilly) Date: Mon Jul 8 21:47:47 2013 Subject: [Histonet] MSI on Ventana Message-ID: <51DC064D.411C.0039.0@health.qld.gov.au> Hello We have previously been sending our MSI Abs to another lab where they are being performed on the Bond. We have now been informed that we are to do our own and one of my IHC staff with previous experience in another lab informs me that they do not work as well on the Ventana platforms that we are currently running. Is ther anybody out there successfully staining MSI on the Ventana. If so what brand/clone of Abs are you using? We are trying to avoid using the Ventana RTU Abs due to cost. regards Tony Tony Reilly B.App.Sc. , M.Sc. Chief Scientist, Anatomical Pathology Pathology Queensland-PA Laboratory ________________________________________________ Health Services Support Agency | Department of Health Level 1, Building 15,Princess Alexandra Hospital Ipswich Road,WOOLLOONGABBA Qld4102 Ph: 07 3176 2412 Mob: 0402 139411 Fax: 07 3176 2930 Email: tony_reilly@health.qld.gov.au Web: www.health.qld.gov.au/qhcss/ ******************************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ********************************************************************************** From LSebree <@t> uwhealth.org Tue Jul 9 07:35:09 2013 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Tue Jul 9 07:35:14 2013 Subject: [Histonet] MSI on Ventana In-Reply-To: <51DC064D.411C.0039.0@health.qld.gov.au> References: <51DC064D.411C.0039.0@health.qld.gov.au> Message-ID: <77DD817201982748BC67D7960F2F76AF050EC0@UWHC-MBX12.uwhis.hosp.wisc.edu> Hey Tony, We are using VMS antibodies (2 are manufactured by CellMarque) on our Ultras. We've had to go with the Optiview detection to get satisfactory results. Let me know if you'd like our protocols. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony Reilly Sent: Monday, July 08, 2013 9:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] MSI on Ventana Hello We have previously been sending our MSI Abs to another lab where they are being performed on the Bond. We have now been informed that we are to do our own and one of my IHC staff with previous experience in another lab informs me that they do not work as well on the Ventana platforms that we are currently running. Is ther anybody out there successfully staining MSI on the Ventana. If so what brand/clone of Abs are you using? We are trying to avoid using the Ventana RTU Abs due to cost. regards Tony Tony Reilly B.App.Sc. , M.Sc. Chief Scientist, Anatomical Pathology Pathology Queensland-PA Laboratory ________________________________________________ Health Services Support Agency | Department of Health Level 1, Building 15,Princess Alexandra Hospital Ipswich Road,WOOLLOONGABBA Qld4102 Ph: 07 3176 2412 Mob: 0402 139411 Fax: 07 3176 2930 Email: tony_reilly@health.qld.gov.au Web: www.health.qld.gov.au/qhcss/ ******************************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ********************************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mtighe <@t> trudeauinstitute.org Tue Jul 9 09:38:18 2013 From: mtighe <@t> trudeauinstitute.org (Mike Tighe) Date: Tue Jul 9 09:38:36 2013 Subject: [Histonet] Paraplast vs. Paraplast plus Message-ID: <8150f333f52b4ab78e125c893005c7bf@BY2PR07MB074.namprd07.prod.outlook.com> Does anyone have a preference over Paraplast and Paraplast plus for mouse tissues? Thanks!! Mike From nguy0515 <@t> gmail.com Tue Jul 9 12:05:57 2013 From: nguy0515 <@t> gmail.com (Trini) Date: Tue Jul 9 12:06:01 2013 Subject: [Histonet] Plastics- please help! Message-ID: <4975CA99-6512-43D4-B65E-A4648ABA2D22@gmail.com> Hello histonetters, Our lab is thinking about introducing plastics but we are not sure what we need or how to set it up. Basically everything on how to set up a plastics lab. I don't know anything about it but would love to learn. Can someone please help us! From PIXLEYSK <@t> UCMAIL.UC.EDU Tue Jul 9 12:23:45 2013 From: PIXLEYSK <@t> UCMAIL.UC.EDU (Pixley, Sarah (pixleysk)) Date: Tue Jul 9 12:23:50 2013 Subject: [Histonet] RE: Plastic/Plexiglass Incubation chamber for IHC slides Message-ID: <944784C5518B3345820A2D4D91A9C7CB458E9A12@UCMAILA6.ad.uc.edu> Dear All: We use a Tupperware container, with a nice air/liquid-tight lid. But any container like that will work. Our trick is to buy plastic ceiling tile material from the hardware store. (Used in ceiling panels.) It comes in big sheets (5 feet by 3 feet?) and is pretty cheap (~$12/sheet). We get the white or clear kind that has square holes cut through the plastic. The holes are about 1 cm square. We then use pliers to "cut" out panels/pieces that will fit inside the container (wear face protection because the plastic flies all over the place). We put one piece in the bottom of the container, then set a metal slide rack (or any water-proof slide rack) on top. We put water in the bottom and this first panel keeps the slides from getting wet. Then, I cut out 4 single squares of the material and put them inside the metal slide racks, at all 4 corners. Then I can lay another metal slide rack on top. Another 4 pieces, another rack, etc. So you can build up a tall stack depending on the size of your container. And the ceiling tile material is also really good for under drying dishes and other uses around the lab. The cheap way to make an incubation chamber! Sarah Pixley Univ. of Cincinnati From reetzba <@t> dxandtx.com Tue Jul 9 12:32:04 2013 From: reetzba <@t> dxandtx.com (Reetz, Brittany (DTC)) Date: Tue Jul 9 12:32:12 2013 Subject: [Histonet] Validating a new reagent Message-ID: My lab is considering getting our xylene and formalin from a different company so all of our reagents are purchase through only one company. I know we cannot just start using these new reagents without doing some kind of validation with our processor and stainer, but after thorough searching on CAP I cannot for the life of me find what we need to do to start using these reagents. Can someone give me some direction as to where to find this info, or if anyone knows what to do let me know? Thanks, Brittany Reetz, HT (ASCP)cm Diagnostic and Treatment Center 3401 Cranberry Blvd Weston WI 54476 (715)-393-2081 reetzba@dxandtx.com ________________________________ CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. From Timothy.Morken <@t> ucsfmedctr.org Tue Jul 9 12:34:15 2013 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Tue Jul 9 12:34:28 2013 Subject: [Histonet] RE: Plastic/Plexiglass Incubation chamber for IHC slides In-Reply-To: <944784C5518B3345820A2D4D91A9C7CB458E9A12@UCMAILA6.ad.uc.edu> References: <944784C5518B3345820A2D4D91A9C7CB458E9A12@UCMAILA6.ad.uc.edu> Message-ID: <761E2B5697F795489C8710BCC72141FF086229@ex07.net.ucsf.edu> Sarah, Wow, brings back memories of the good ol' days when we used all kinds of stuff to make our own trays. In one lab I worked at we had a small Tupperware container for each antibody and layed paper towels and wood rods on the bottom to hold the slides the surface. We'd wet the paper towels for humidity. When a new lab was built they specifically designed it for this method and we had a 20-foot long bench just so we could line up dozens of these small Tupperware containers! What a pain that was to go through each round of washing!! We did 150 slides a day that way for years. Blessed is the day we got automated stainers!! Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pixley, Sarah (pixleysk) Sent: Tuesday, July 09, 2013 10:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Plastic/Plexiglass Incubation chamber for IHC slides Dear All: We use a Tupperware container, with a nice air/liquid-tight lid. But any container like that will work. Our trick is to buy plastic ceiling tile material from the hardware store. (Used in ceiling panels.) It comes in big sheets (5 feet by 3 feet?) and is pretty cheap (~$12/sheet). We get the white or clear kind that has square holes cut through the plastic. The holes are about 1 cm square. We then use pliers to "cut" out panels/pieces that will fit inside the container (wear face protection because the plastic flies all over the place). We put one piece in the bottom of the container, then set a metal slide rack (or any water-proof slide rack) on top. We put water in the bottom and this first panel keeps the slides from getting wet. Then, I cut out 4 single squares of the material and put them inside the metal slide racks, at all 4 corners. Then I can lay another metal slide rack on top. Another 4 pieces, another rack, etc. So you can build up a tall stack depending on the size of your container. And the ceiling tile material is also really good for under drying dishes and other uses around the lab. The cheap way to make an incubation chamber! Sarah Pixley Univ. of Cincinnati _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From talulahgosh <@t> gmail.com Tue Jul 9 12:46:13 2013 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Tue Jul 9 12:46:23 2013 Subject: [Histonet] RE: Plastic/Plexiglass Incubation chamber for IHC slides In-Reply-To: <761E2B5697F795489C8710BCC72141FF086229@ex07.net.ucsf.edu> References: <944784C5518B3345820A2D4D91A9C7CB458E9A12@UCMAILA6.ad.uc.edu> <761E2B5697F795489C8710BCC72141FF086229@ex07.net.ucsf.edu> Message-ID: We use bioassay dishes with 10mL pipets glued to them. The slides sit on two pipets and you can get two rows of slides in. Cutting the pipets' ends off is the hardest part. I take that back, finding a glue that will hold the pipets has been a problem lately, as rubber cement isn't made like they used to make it. A glue gun works, but sometimes the pipets need to be reglued. Emily "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." -Chuck Palahniuk, "Haunted" On Tue, Jul 9, 2013 at 1:34 PM, Morken, Timothy < Timothy.Morken@ucsfmedctr.org> wrote: > Sarah, > > Wow, brings back memories of the good ol' days when we used all kinds of > stuff to make our own trays. In one lab I worked at we had a small > Tupperware container for each antibody and layed paper towels and wood rods > on the bottom to hold the slides the surface. We'd wet the paper towels for > humidity. When a new lab was built they specifically designed it for this > method and we had a 20-foot long bench just so we could line up dozens of > these small Tupperware containers! What a pain that was to go through each > round of washing!! We did 150 slides a day that way for years. Blessed is > the day we got automated stainers!! > > > Tim Morken > Supervisor, Electron Microscopy/Neuromuscular Special Studies > Department of Pathology > UC San Francisco Medical Center > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pixley, Sarah > (pixleysk) > Sent: Tuesday, July 09, 2013 10:24 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: Plastic/Plexiglass Incubation chamber for IHC > slides > > Dear All: > We use a Tupperware container, with a nice air/liquid-tight lid. But any > container like that will work. Our trick is to buy plastic ceiling tile > material from the hardware store. (Used in ceiling panels.) It comes in > big sheets (5 feet by 3 feet?) and is pretty cheap (~$12/sheet). We get > the white or clear kind that has square holes cut through the plastic. The > holes are about 1 cm square. We then use pliers to "cut" out panels/pieces > that will fit inside the container (wear face protection because the > plastic flies all over the place). We put one piece in the bottom of the > container, then set a metal slide rack (or any water-proof slide rack) on > top. We put water in the bottom and this first panel keeps the slides from > getting wet. Then, I cut out 4 single squares of the material and put them > inside the metal slide racks, at all 4 corners. Then I can lay another > metal slide rack on top. Another 4 pieces, another rack, etc. So you can > build up a tall stack depending on the size of your container. And the > ceiling tile material is also really good for under drying dishes and other > uses around the lab. > The cheap way to make an incubation chamber! > Sarah Pixley > Univ. of Cincinnati > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From lcolbert <@t> pathmdlabs.com Tue Jul 9 12:49:38 2013 From: lcolbert <@t> pathmdlabs.com (Laurie Colbert) Date: Tue Jul 9 12:51:24 2013 Subject: [Histonet] p16 control tissue Message-ID: <12ECD7346266D74691EC2BFC75285E452F3135F9@BFL323E10.pathmdlabs.local> We are going to be running the p16 antibody from Biocare (RUO) on skin specimens. What type of tissue should I run for a positive control? The data sheet says normal testis.......... Laurie Colbert From JMacDonald <@t> mtsac.edu Tue Jul 9 12:56:33 2013 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Tue Jul 9 12:56:46 2013 Subject: [Histonet] immuno moisture chambers Message-ID: Ted Pella has some options. We use the Immunostain Moisture Chambers #21049, but there are others. http://www.tedpella.com/glasswar_html/slidedsh.htm#21049 From LSebree <@t> uwhealth.org Tue Jul 9 12:58:11 2013 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Tue Jul 9 12:58:14 2013 Subject: [Histonet] RE: p16 control tissue In-Reply-To: <12ECD7346266D74691EC2BFC75285E452F3135F9@BFL323E10.pathmdlabs.local> References: <12ECD7346266D74691EC2BFC75285E452F3135F9@BFL323E10.pathmdlabs.local> Message-ID: <77DD817201982748BC67D7960F2F76AF051054@UWHC-MBX12.uwhis.hosp.wisc.edu> Laurie, Ideally you would use the same type of tissue as your test tissue. Our validation procedure states that we must run all (within reason) types of tissue that a particular antibody will be used with. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Tuesday, July 09, 2013 12:50 PM To: Histonet Post (histonet@lists.utsouthwestern.edu) Subject: [Histonet] p16 control tissue We are going to be running the p16 antibody from Biocare (RUO) on skin specimens. What type of tissue should I run for a positive control? The data sheet says normal testis.......... Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TMcNemar <@t> lmhealth.org Tue Jul 9 12:58:56 2013 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Tue Jul 9 12:58:51 2013 Subject: [Histonet] RE: p16 control tissue In-Reply-To: <12ECD7346266D74691EC2BFC75285E452F3135F9@BFL323E10.pathmdlabs.local> References: <12ECD7346266D74691EC2BFC75285E452F3135F9@BFL323E10.pathmdlabs.local> Message-ID: We are using Biocare's ab with squamous cell ca as the control of choice. I believe you can also use cervical dysplasia or tonsil. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Tuesday, July 09, 2013 1:50 PM To: Histonet Post (histonet@lists.utsouthwestern.edu) Subject: [Histonet] p16 control tissue We are going to be running the p16 antibody from Biocare (RUO) on skin specimens. What type of tissue should I run for a positive control? The data sheet says normal testis.......... Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From dhewitt <@t> hvhs.org Tue Jul 9 13:08:42 2013 From: dhewitt <@t> hvhs.org (Daniel Hewitt) Date: Tue Jul 9 13:08:49 2013 Subject: [Histonet] RE: p16 control tissue In-Reply-To: Message-ID: <7DDB5AB36CBC574D8D680806E7BBE58B0169453C@MX-HVB-02.hvhs.org> We use a nice CIN3 on a cervical cone. Daniel Hewitt Histology Supervisor, HVS 412-749-7371 This email, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, or an agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and delete and destroy all copies of the original message, including attachments. Please note that any views or opinions presented in this e-mail are solely those of the author and do not necessarily represent those of Heritage Valley Health System. The integrity and security of this message cannot be guaranteed on the internet. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Tuesday, July 09, 2013 1:59 PM To: 'Laurie Colbert'; Histonet Post(histonet@lists.utsouthwestern.edu) Subject: [Histonet] RE: p16 control tissue We are using Biocare's ab with squamous cell ca as the control of choice. I believe you can also use cervical dysplasia or tonsil. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Tuesday, July 09, 2013 1:50 PM To: Histonet Post (histonet@lists.utsouthwestern.edu) Subject: [Histonet] p16 control tissue We are going to be running the p16 antibody from Biocare (RUO) on skin specimens. What type of tissue should I run for a positive control? The data sheet says normal testis.......... Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From KSimeone <@t> leavittmgt.com Tue Jul 9 13:41:33 2013 From: KSimeone <@t> leavittmgt.com (Delray Beach Pathology Kari Simeone) Date: Tue Jul 9 13:41:43 2013 Subject: [Histonet] TEMP POSITION PALM BEACH, FL Message-ID: Hi...We're looking for a Histotechnologist for a 3-4 month temporary (possible full-time) position at our very busy dermatology lab in Delray Beach, FL. THIS IS NOT AN AGENCY. Please contact me below or send your resume if interested. This would be a 8a-5p, full time position from July-November. Here is our current advertisement. Job Duties Include: Grossing, processing, embedding, cutting, staining and coverslipping. Accessioning all specimens along with other team members. Attention to detail. Performing special stains, following protocols. Putting slides together with requisitions- double checking for accuracy. Cutting control slides Quality Control Adhering to all Policy and Procedures Job Requirements Requirements Include: Current Florida Histotechnologist license A MINIMUM of Five years + experience in the following: Embedding Cutting Staining Special Stains Troubleshooting Immunohistochemistry- 2 years + experience preferred. Grossing experience preferred, but will train. PLEASE DO NOT CONTACT ME FROM ANY AGENCIES, YOU WILL NOT BE ANSWERED. Thanks! Kari M Simeone Histology/Immunohistochemistry Specialist Supervisor Alternate Laboratory Supervisor ADCS Clinics, LLC Advanced Dermatology and Cosmetic Surgery Ameriderm 561.819.0857 ext 100 561.819.6517 fax ksimeone@leavittmgt.com www.advancedderm.com The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message. From talulahgosh <@t> gmail.com Tue Jul 9 14:17:48 2013 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Tue Jul 9 14:17:53 2013 Subject: [Histonet] a stretch Message-ID: Does anyone know Tony Green at Presby UPMC? I can't find his contact info and UPMC doesn't let you search employees. And if you do, I LOVE YOU AND I NEED A JOB. Emily "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." -Chuck Palahniuk, "Haunted" From deganh <@t> upstate.edu Tue Jul 9 14:33:28 2013 From: deganh <@t> upstate.edu (Helene Degan) Date: Tue Jul 9 14:33:33 2013 Subject: [Histonet] COX/SDH Combo staining Message-ID: <51DC2D480200007A000255AC@gatedom1.upstate.edu> Hi I'm looking for COX/SDH combo staining procedures for muscle enzymes we currently do these two enzyme procedure separately and we our looking into combining them or any idea as to what sites I may find the information. Thanks Helene deganh@upstate.edu From chesarato <@t> hotmail.com Tue Jul 9 14:39:14 2013 From: chesarato <@t> hotmail.com (Cesar Francisco Romero) Date: Tue Jul 9 14:39:22 2013 Subject: [Histonet] RE: Plastic/Plexiglass Incubation chamber for IHC slides Message-ID: For Manual IHC I suggest best the Sequenza Slide Rack from Thermo Scientific. It makes your life easier and you can save money, because you only need 100 ul of any reactive per slide. Here is the link to the web page. http://www.thermo.fi/com/cda/product/detail/0,1055,1000005630785,00.html I hope it helps. Cesar Romero Buenos Aires Argentina From sdysart <@t> mirnarx.com Tue Jul 9 14:49:25 2013 From: sdysart <@t> mirnarx.com (Sarah Dysart) Date: Tue Jul 9 14:49:48 2013 Subject: [Histonet] IHC controls Message-ID: <6b9539e768184c8ab8449206dbc2dbb8@BY2PR07MB106.namprd07.prod.outlook.com> So, I am working on some IHC stuff. I need control for mouse tissues. While I have just about every normal tissue you could ask for, several of these antibodies say the positive control is lung cancer, or breast cancer, or prostate cancer. While I have all of these in human I don't have them in the species I am staining in...mouse. Also, a couple of them say tonsil...do mice even have tonsils?? Anyhoo, what is ya'lls suggestion on this one? Do I stain the appropriate tissue just in the wrong species (human), or does someone know where I can purchase mouse control slides and/or blocks? Thanks Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 From jamie.erickson <@t> abbvie.com Tue Jul 9 15:52:32 2013 From: jamie.erickson <@t> abbvie.com (Erickson, Jamie E) Date: Tue Jul 9 15:52:37 2013 Subject: [Histonet] Section cells on a plastic trans well membrane Message-ID: <8B946A68A8F3534A99CC493DEFB49B101325A98A@WM10002P.oneabbott.com> Hi All, Has anyone tried to section cells cut from a trans well dish? We have cells that are cultured and attach to a trans well membrane and our pathologist wants to look at them on the membrane but the membrane is a thin plastic film...see the problem. We fixed the cells in the trans well for 5 minutes then cut the trans well out of the well and bisected it and processed overnight we use paraffin type #1 paraffin. The next morning we embedded it with type #9 paraffin. The samples cut but do not stay on the slide after xylene. I think the plastic does not adhere to the slide. Would a adhesive slide work to keep the thin plastic stuck to the slide?? My pathologist does not want to change to a collagen membrane so he wants me to find another (harder paraffin) to use, I don't think that will help. Has anyone does similar processing/embedding? I'll take any help I can get.. Thanks, Jamie Abbvie Bioreseach Center Pharmacology 100 Research Dr. Worcester,Ma 01605 OFFICE +1 508-688-3134 FAX +1 508-793-4895 EMAIL jamie.erickson@abbvie.com From liz <@t> premierlab.com Tue Jul 9 16:05:28 2013 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Tue Jul 9 16:05:33 2013 Subject: [Histonet] RE: Section cells on a plastic trans well membrane In-Reply-To: <8B946A68A8F3534A99CC493DEFB49B101325A98A@WM10002P.oneabbott.com> References: <8B946A68A8F3534A99CC493DEFB49B101325A98A@WM10002P.oneabbott.com> Message-ID: <14E2C6176416974295479C64A11CB9AE016411AB4A03@SBS2K8.premierlab.local> Jamie We have sectioned these before and we used charged slides and did not have a problem with section adherence. I would fix longer than 5 minutes. We would use biopsy punches to remove the membrane from the well we would process the disc whole between biopsy pads and bisect prior to embedding. You need to use a good plus slide and it you are still having problems I would let the unstained slides sit for a couple days prior to staining. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 881-0763 cell (303) 682-9060 fax liz@premierlab.com Ship to address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Erickson, Jamie E [jamie.erickson@abbvie.com] Sent: Tuesday, July 09, 2013 2:52 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Section cells on a plastic trans well membrane Hi All, Has anyone tried to section cells cut from a trans well dish? We have cells that are cultured and attach to a trans well membrane and our pathologist wants to look at them on the membrane but the membrane is a thin plastic film...see the problem. We fixed the cells in the trans well for 5 minutes then cut the trans well out of the well and bisected it and processed overnight we use paraffin type #1 paraffin. The next morning we embedded it with type #9 paraffin. The samples cut but do not stay on the slide after xylene. I think the plastic does not adhere to the slide. Would a adhesive slide work to keep the thin plastic stuck to the slide?? My pathologist does not want to change to a collagen membrane so he wants me to find another (harder paraffin) to use, I don't think that will help. Has anyone does similar processing/embedding? I'll take any help I can get.. Thanks, Jamie Abbvie Bioreseach Center Pharmacology 100 Research Dr. Worcester,Ma 01605 OFFICE +1 508-688-3134 FAX +1 508-793-4895 EMAIL jamie.erickson@abbvie.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Tue Jul 9 17:42:52 2013 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Tue Jul 9 17:51:34 2013 Subject: [Histonet] Wage and Vacancy Survey Message-ID: I was able to locate the vacancy survey that the ASCP published, but cannot find the wage survey. Does anyone have information? It was supposed to be published in the November 2012 issue of LabMedicine. Thanks, Jennifer MacDonald From tony.henwood <@t> health.nsw.gov.au Tue Jul 9 18:17:48 2013 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Tue Jul 9 18:18:06 2013 Subject: [Histonet] COX/SDH Combo staining In-Reply-To: <51DC2D480200007A000255AC@gatedom1.upstate.edu> References: <51DC2D480200007A000255AC@gatedom1.upstate.edu> Message-ID: <6D6BD1DE8A5571489398B392A38A71579D28AE05@xmdb04.nch.kids> Here is our technique: Cytochrome Oxidase - SDH Use 1. Defects of cytochrome oxidase activity 2. Demonstration of mitochondria Underlying Principle Combining the COX with the SDH can demonstrate COX negative fibres. These may be SDH positive and may be ragged red fibres. 3 of the 13 subunits of COX are encoded by mitochondrial DNA whereas SDH is encoded by nuclear DNA. Therefore SDH is not affected by mitochondrial DNA mutations. Ragged red fibres in mitochondrial myopathies are generally COX neg (except in MELAS). Intra fibre mosaicism (mixture of bluish COX deficient and brownish COX positive mitochondria within the same fibre are also well demonstrated by this combined technique). Fixation and Sectioning Air dried unfixed 8?M cryostat sections Reagents 1. PBS Buffer: Dissolve one Dulbecco PBS Tablet in 100ml distilled water 2. COX A Solution: Warning: DAB is carcinogenic ? Avoid contact with skin Sucrose 0.75g 3,3' Diaminobenzidine.4HCl (DAB) 0.05g Adjust to pH 7.6 PBS Buffer up to 50ml Aliquot in 2ml amounts (labelled CA), store at -20oC 3. COX B Solution: Catalase (Sigma C9322) 0.001g Cytochrome C (Sigma C2037) 0.05g Adjust to pH 7.6 PBS Buffer up to 50ml Aliquot in 2ml amounts (labelled CB), store at -20oC 4. Incubating medium Defrost one CA and one CB vial, mix and place in a 2-slide plastic slide mailer. Place mailer in a coplin jar for support. 5. SDH Reagents 1 Stock 0.2M Sodium Dihydrogen Phosphate (NaH2PO4) Sodium Dihydrogen Phosphate 23.996 g Distilled water 1000 ml Store at room temperature 2 Stock 0.2M Disodium Hydrogen Phosphate (Na2HPO4) Disodium Hydrogen Phosphate 28.392 g Distilled water 1000 ml Store at room temperature 3 0.6M succinate solution (adjusted to pH 7.6) Warning: Irritant ? see MSDS Sodium succinate 12.96 g Distilled water 64.00 ml 1M HCl 0.40 ml pH to 7.6 with 0.2M Disodium Hydrogen Phosphate Distilled water up to 80 ml Aliquot 800?l into eppendorf vials (labelled S) and store at -20?C 3. Yellow SDH Incubation Medium Stock 0.2M Sodium Hydrogen Phosphate 52ml Stock 0.2M Disodium Hydrogen Phosphate 348ml Nitro Blue Tetrazolium (NBT) 0.6 g Adjust to pH 7.6 with stock phosphate solutions Aliquot 4ml into tubes labelled ?SY? and store at -20?C (enough for 100 tubes) 4. Incubating medium (prepare fresh) 1. Defrost a vial of succinate solution (S) and a Yellow Incubation Solution (SY) 2. Add solution ?S? to ?SY?, prior to use, mix and place in a small plastic slide mailer. Method 1. Prepare incubation solution 2. Immediately place frozen sectioned slides in incubation solution and incubate for two hours at room temperature. 3. Check staining and replace for longer if required 4. Rinse slides in distilled water and prepare SDH incubation medium. 5. Add slides to SDH medium and incubate at 37oC for 1-2 hour. 6. Rinse slides in distilled water. 7. Rinse in water, dehydrate, clear and mount. Results Cytochrome Oxidase positive mitochondria Brown. Cytochrome Oxidase negative mitochondria Blue References 1. Seligman etal (1968) J Cell Biol 38:1-14. 2. Loughlin M. (1993). Muscle biopsy. A laboratory investigation. Butterworth-Heinemann p.38-39. 3. Sheehan D, Hrapchak B. (1987). Histotechnology, 2nd Ed. Batelle Press, Columbus p306-307 Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Helene Degan Sent: Wednesday, 10 July 2013 5:33 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] COX/SDH Combo staining Hi I'm looking for COX/SDH combo staining procedures for muscle enzymes we currently do these two enzyme procedure separately and we our looking into combining them or any idea as to what sites I may find the information. Thanks Helene deganh@upstate.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From batesf <@t> ohsu.edu Tue Jul 9 18:29:45 2013 From: batesf <@t> ohsu.edu (Florence Leomiti) Date: Tue Jul 9 18:29:51 2013 Subject: [Histonet] COX/SDH Combo staining In-Reply-To: <6D6BD1DE8A5571489398B392A38A71579D28AE05@xmdb04.nch.kids> References: <51DC2D480200007A000255AC@gatedom1.upstate.edu> <6D6BD1DE8A5571489398B392A38A71579D28AE05@xmdb04.nch.kids> Message-ID: <311B5F326A1C0E4D8CACC6F278FCACEA1667A9A1D8@EX-MB07.ohsu.edu> Easier way to do this stain...since you do these two stains separately incubate the slide in a the COX solution for 1 hour rinse in distilled water and incubate in SDH solution for 1 hr. rinse 5min in physiological saline, 10min in 10% formalin saline, rinse in 15% ethanol then mount with Crystal mount media. Let dry then cover slip as usual Florence Leomiti??? HT (ASCP) Neuromuscular Lab Tech. Phone 503-494-6781 Fax 503-418-4249 Pager 16822 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony Henwood (SCHN) Sent: Tuesday, July 09, 2013 4:18 PM To: 'Helene Degan'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] COX/SDH Combo staining Here is our technique: Cytochrome Oxidase - SDH Use 1. Defects of cytochrome oxidase activity 2. Demonstration of mitochondria Underlying Principle Combining the COX with the SDH can demonstrate COX negative fibres. These may be SDH positive and may be ragged red fibres. 3 of the 13 subunits of COX are encoded by mitochondrial DNA whereas SDH is encoded by nuclear DNA. Therefore SDH is not affected by mitochondrial DNA mutations. Ragged red fibres in mitochondrial myopathies are generally COX neg (except in MELAS). Intra fibre mosaicism (mixture of bluish COX deficient and brownish COX positive mitochondria within the same fibre are also well demonstrated by this combined technique). Fixation and Sectioning Air dried unfixed 8?M cryostat sections Reagents 1. PBS Buffer: Dissolve one Dulbecco PBS Tablet in 100ml distilled water 2. COX A Solution: Warning: DAB is carcinogenic ? Avoid contact with skin Sucrose 0.75g 3,3' Diaminobenzidine.4HCl (DAB) 0.05g Adjust to pH 7.6 PBS Buffer up to 50ml Aliquot in 2ml amounts (labelled CA), store at -20oC 3. COX B Solution: Catalase (Sigma C9322) 0.001g Cytochrome C (Sigma C2037) 0.05g Adjust to pH 7.6 PBS Buffer up to 50ml Aliquot in 2ml amounts (labelled CB), store at -20oC 4. Incubating medium Defrost one CA and one CB vial, mix and place in a 2-slide plastic slide mailer. Place mailer in a coplin jar for support. 5. SDH Reagents 1 Stock 0.2M Sodium Dihydrogen Phosphate (NaH2PO4) Sodium Dihydrogen Phosphate 23.996 g Distilled water 1000 ml Store at room temperature 2 Stock 0.2M Disodium Hydrogen Phosphate (Na2HPO4) Disodium Hydrogen Phosphate 28.392 g Distilled water 1000 ml Store at room temperature 3 0.6M succinate solution (adjusted to pH 7.6) Warning: Irritant ? see MSDS Sodium succinate 12.96 g Distilled water 64.00 ml 1M HCl 0.40 ml pH to 7.6 with 0.2M Disodium Hydrogen Phosphate Distilled water up to 80 ml Aliquot 800?l into eppendorf vials (labelled S) and store at -20?C 3. Yellow SDH Incubation Medium Stock 0.2M Sodium Hydrogen Phosphate 52ml Stock 0.2M Disodium Hydrogen Phosphate 348ml Nitro Blue Tetrazolium (NBT) 0.6 g Adjust to pH 7.6 with stock phosphate solutions Aliquot 4ml into tubes labelled ?SY? and store at -20?C (enough for 100 tubes) 4. Incubating medium (prepare fresh) 1. Defrost a vial of succinate solution (S) and a Yellow Incubation Solution (SY) 2. Add solution ?S? to ?SY?, prior to use, mix and place in a small plastic slide mailer. Method 1. Prepare incubation solution 2. Immediately place frozen sectioned slides in incubation solution and incubate for two hours at room temperature. 3. Check staining and replace for longer if required 4. Rinse slides in distilled water and prepare SDH incubation medium. 5. Add slides to SDH medium and incubate at 37oC for 1-2 hour. 6. Rinse slides in distilled water. 7. Rinse in water, dehydrate, clear and mount. Results Cytochrome Oxidase positive mitochondria Brown. Cytochrome Oxidase negative mitochondria Blue References 1. Seligman etal (1968) J Cell Biol 38:1-14. 2. Loughlin M. (1993). Muscle biopsy. A laboratory investigation. Butterworth-Heinemann p.38-39. 3. Sheehan D, Hrapchak B. (1987). Histotechnology, 2nd Ed. Batelle Press, Columbus p306-307 Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Helene Degan Sent: Wednesday, 10 July 2013 5:33 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] COX/SDH Combo staining Hi I'm looking for COX/SDH combo staining procedures for muscle enzymes we currently do these two enzyme procedure separately and we our looking into combining them or any idea as to what sites I may find the information. Thanks Helene deganh@upstate.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From joelleweaver <@t> hotmail.com Tue Jul 9 18:38:01 2013 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Tue Jul 9 18:38:07 2013 Subject: [Histonet] Wage and Vacancy Survey In-Reply-To: References: Message-ID: I guess every other year. This is 2010 http://labmed.ascpjournals.org/content/42/3/141.full.pdf+html The last couple of years vacancy & wage from ASCP site http://www.ascp.org/functional-nav/career-center Joelle Weaver MAOM, HTL (ASCP) QIHC > To: histonet@lists.utsouthwestern.edu > From: JMacDonald@mtsac.edu > Date: Tue, 9 Jul 2013 15:42:52 -0700 > Subject: [Histonet] Wage and Vacancy Survey > > I was able to locate the vacancy survey that the ASCP published, but > cannot find the wage survey. Does anyone have information? It was > supposed to be published in the November 2012 issue of LabMedicine. > Thanks, > Jennifer MacDonald > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Nancy_Schmitt <@t> pa-ucl.com Wed Jul 10 04:30:37 2013 From: Nancy_Schmitt <@t> pa-ucl.com (Nancy Schmitt) Date: Wed Jul 10 04:30:43 2013 Subject: [Histonet] AFB stain Message-ID: <906B4DA90ED1DB4DB6C7E94D7CEE6C36813D6091@PEITHA.wad.pa-ucl.com> We run the AFB on the Dako Artisan - recently it has been coming off with VERY intense blue staining. Tech. support offered a new kit - same problem. We lessened the time and got the same result. All other stains on the Artisan are working. Thoughts are appreciated! Nancy Schmitt HT, MLT(ASCP) Histology Coordinator United Clinical Laboratories Dubuque, IA 52001 NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From jqb7 <@t> cdc.gov Wed Jul 10 04:45:42 2013 From: jqb7 <@t> cdc.gov (Sanders, Jeanine (CDC/OID/NCEZID)) Date: Wed Jul 10 04:45:57 2013 Subject: [Histonet] RE: AFB stain In-Reply-To: <906B4DA90ED1DB4DB6C7E94D7CEE6C36813D6091@PEITHA.wad.pa-ucl.com> References: <906B4DA90ED1DB4DB6C7E94D7CEE6C36813D6091@PEITHA.wad.pa-ucl.com> Message-ID: We have been using the AFB Green from Dako but prior to that no issues with the blue Jeanine H. Sanders Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 404-639-3590 jqb7@cdc.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt Sent: Wednesday, July 10, 2013 5:31 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] AFB stain We run the AFB on the Dako Artisan - recently it has been coming off with VERY intense blue staining. Tech. support offered a new kit - same problem. We lessened the time and got the same result. All other stains on the Artisan are working. Thoughts are appreciated! Nancy Schmitt HT, MLT(ASCP) Histology Coordinator United Clinical Laboratories Dubuque, IA 52001 NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ranna0726 <@t> gmail.com Wed Jul 10 08:17:47 2013 From: ranna0726 <@t> gmail.com (Ranna Mehta) Date: Wed Jul 10 08:17:53 2013 Subject: [Histonet] re.COX/SDH Combo staining Message-ID: Hi Helene, I have the procedure for cox/sdh staining. I stains frozen muscle first with COX procedure and then SDH. I will send you in your private email. Regards Ranna Neuromuscular dept. From algranth <@t> email.arizona.edu Wed Jul 10 09:40:20 2013 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Wed Jul 10 09:40:26 2013 Subject: [Histonet] Section cells on a plastic trans well membrane In-Reply-To: <8B946A68A8F3534A99CC493DEFB49B101325A98A@WM10002P.oneabbott.com> References: <8B946A68A8F3534A99CC493DEFB49B101325A98A@WM10002P.oneabbott.com> Message-ID: <8D510418-84BE-4CF5-A5D7-9FBFECC47618@email.arizona.edu> I do these frequently. The lab requesting the work usually does the fixing but I'm sure they do longer than 5 minutes. They then replace the fixative with 70% ETOH and bring the whole thing to the lab. I use a tip of a scalpel blade to remove the whole membrane and I process it in a screen cassette or a bx cassette with small holes. I don't like to put anything on top of the membrane that might disturb the cells growing on it. When embedding I cut the membrane circle in half and embed it standing up with the cut side down. I always use charged slides and have done H&E's and various special stains on the membranes and have never had one lift off. I usually let dry overnite and then put the slides in a drying oven with gentle heat for maybe 15 minutes before staining. Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 From sforeman <@t> labpath.com Wed Jul 10 11:24:39 2013 From: sforeman <@t> labpath.com (Susan Foreman) Date: Wed Jul 10 11:27:30 2013 Subject: [Histonet] BRAF IHC Message-ID: <004301ce7d89$fa2344d0$ee69ce70$@com> Pat, I was wondering how your BRAF IHC staining is going? We have the same antibody working using Ventana Benchmark Ultra. We do use amp kit. With you post, did anyone respond that was using other clones? V600K or D? Many Thanks, Susan Foreman (865)584-1933 From john <@t> imebinc.com Wed Jul 10 11:32:51 2013 From: john <@t> imebinc.com (john@imebinc.com) Date: Wed Jul 10 11:34:48 2013 Subject: [Histonet] bubbles in paraffin blocks Message-ID: <2AE4B22D615240F4B9D5D3E514315801@IMEBJOHN> Histonet experts,can you give me reason why embedded paraffin would have tiny bubbles in the block after the cooling process? Any idea are appreciated John O'Brien IMEB, INC "The Pathology Specialists" Tel 800-543-8496 Fax:760-761-0859 john@imebinc.com From michaela.tourville <@t> duke.edu Wed Jul 10 11:38:56 2013 From: michaela.tourville <@t> duke.edu (Michaela Lefaivre) Date: Wed Jul 10 11:39:06 2013 Subject: [Histonet] RE: BRAF IHC Message-ID: Any luck getting the Braf IHC to work using biocare? I am getting ready to work this antibody up. Thanks in advance Michaela Michaela LeFaivre BS, HTL (ASCP) CM Molecular Technician III Molecular Pathology Rm 4344 Purple Zone 919-684-4303 HIPAA Privacy Notification: This message and any accompanying documents are covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, and contain information intended for the specific individual (s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying or the taking of any action based on the contents of this information is strictly prohibited . If you have received this communication in error, please notify us immediately by e-mail , and delete the original message. From Pat.Bell <@t> ucdenver.edu Wed Jul 10 11:52:49 2013 From: Pat.Bell <@t> ucdenver.edu (Bell, Pat) Date: Wed Jul 10 11:53:03 2013 Subject: [Histonet] BRAF IHC In-Reply-To: <004301ce7d89$fa2344d0$ee69ce70$@com> References: <004301ce7d89$fa2344d0$ee69ce70$@com> Message-ID: <64DB27005E2FD3439E88502D7A5C9121010241E276C2@CORTEZ.ucdenver.pvt> I have not used this antibody so I don't know what you mean. Maybe I am missing something! Sorry, Pat -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Susan Foreman Sent: Wednesday, July 10, 2013 10:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] BRAF IHC Pat, I was wondering how your BRAF IHC staining is going? We have the same antibody working using Ventana Benchmark Ultra. We do use amp kit. With you post, did anyone respond that was using other clones? V600K or D? Many Thanks, Susan Foreman (865)584-1933 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TMcNemar <@t> lmhealth.org Wed Jul 10 12:01:01 2013 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Wed Jul 10 12:00:54 2013 Subject: [Histonet] bubbles in paraffin blocks In-Reply-To: <2AE4B22D615240F4B9D5D3E514315801@IMEBJOHN> References: <2AE4B22D615240F4B9D5D3E514315801@IMEBJOHN> Message-ID: I see this when using the cassettes with the smaller holes and when the paraffin is added too fast. Air gets trapped under the cassette. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of john@imebinc.com Sent: Wednesday, July 10, 2013 12:33 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] bubbles in paraffin blocks Histonet experts,can you give me reason why embedded paraffin would have tiny bubbles in the block after the cooling process? Any idea are appreciated John O'Brien IMEB, INC "The Pathology Specialists" Tel 800-543-8496 Fax:760-761-0859 john@imebinc.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From sforeman <@t> labpath.com Wed Jul 10 12:04:36 2013 From: sforeman <@t> labpath.com (Susan Foreman) Date: Wed Jul 10 12:07:29 2013 Subject: [Histonet] BRAF IHC In-Reply-To: <64DB27005E2FD3439E88502D7A5C9121010241E276C2@CORTEZ.ucdenver.pvt> References: <004301ce7d89$fa2344d0$ee69ce70$@com> <64DB27005E2FD3439E88502D7A5C9121010241E276C2@CORTEZ.ucdenver.pvt> Message-ID: <004901ce7d8f$8e96b5c0$abc42140$@com> I was attempting to respond to this older post: Thanks Pat Zeitlow pzeitlow <@t> bbpllab.com Fri May 3 13:52:49 CDT 2013 Previous message: [Histonet] BRAF IHC Next message: [Histonet] BrDU Messages sorted by: [ date ] [ thread ] [ subject ] [ author ] Also using Spring Bioscience antibody. Trying to get it automated using Biocare instrument. You? Pat -----Original Message----- From: Bitting, Angela K. [mailto:akbitting <@t> geisinger.edu] Sent: Friday, May 03, 2013 1:45 PM To: Pat Zeitlow Subject: RE: BRAF IHC Working it up right now. Using Spring Bioscience's BRAF V600E. Are you using an automated platform? Angie -----Original Message----- From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Pat Zeitlow Sent: Friday, May 03, 2013 2:36 PM To: histonet <@t> lists.utsouthwestern.edu Subject: [Histonet] BRAF IHC Is anyone doing BRAF IHC staining that is willing to share protocol details? TIA, Pat From CDavis <@t> che-east.org Wed Jul 10 12:44:25 2013 From: CDavis <@t> che-east.org (Davis, Cassie) Date: Wed Jul 10 12:45:39 2013 Subject: [Histonet] bubbles in paraffin blocks & darker blue counterstain In-Reply-To: References: Message-ID: <08861B9CF6C7774E874635A4818AE37B0122FD966C@CHEXCMS01.one.ads.che.org> RE: tiny bubbles If the tiny bubbles are a "new" thing it might be humidity causing tiny water droplets to condense in your melted paraffin or on the paraffin pellets/flakes in transport to your lab or in your lab that show up after melting. RE: too dark AFB blue counterstain I don't know the auto-stainer you use or the protocol you follow after staining but might not be the machine. If you dehydrate & clear your slides by hand after staining it might be the alcohols. Some techs are known to use absolute alcohol where the 95% is supposed to be in dehydrating before clearing steps. If that is the case, not as much of the blue is removed during the process. Cassandra Davis CDavis@che-east.org 302-575-8095 Saint Francis Hospital Saintfrancishealthcare.org Saint Francis Facebook Page ________________________________________ Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From gu.lang <@t> gmx.at Wed Jul 10 13:02:14 2013 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Wed Jul 10 13:26:45 2013 Subject: AW: [Histonet] bubbles in paraffin blocks In-Reply-To: <2AE4B22D615240F4B9D5D3E514315801@IMEBJOHN> References: <2AE4B22D615240F4B9D5D3E514315801@IMEBJOHN> Message-ID: <001b01ce7d97$9cf70090$d6e501b0$@gmx.at> I've seen this with paraffin, that was too long in the oven before embedding. After (I think) weeks it comes to saponification of the paraffin chains. So it makes bubbles like soap. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von john@imebinc.com Gesendet: Mittwoch, 10. Juli 2013 18:33 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] bubbles in paraffin blocks Histonet experts,can you give me reason why embedded paraffin would have tiny bubbles in the block after the cooling process? Any idea are appreciated John O'Brien IMEB, INC "The Pathology Specialists" Tel 800-543-8496 Fax:760-761-0859 john@imebinc.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Pat.Bell <@t> ucdenver.edu Wed Jul 10 13:32:31 2013 From: Pat.Bell <@t> ucdenver.edu (Bell, Pat) Date: Wed Jul 10 13:32:42 2013 Subject: [Histonet] BRAF IHC In-Reply-To: <004901ce7d8f$8e96b5c0$abc42140$@com> References: <004301ce7d89$fa2344d0$ee69ce70$@com> <64DB27005E2FD3439E88502D7A5C9121010241E276C2@CORTEZ.ucdenver.pvt> <004901ce7d8f$8e96b5c0$abc42140$@com> Message-ID: <64DB27005E2FD3439E88502D7A5C9121010241E2779B@CORTEZ.ucdenver.pvt> OH, Wrong Pat! :) -----Original Message----- From: Susan Foreman [mailto:sforeman@labpath.com] Sent: Wednesday, July 10, 2013 11:05 AM To: Bell, Pat; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] BRAF IHC I was attempting to respond to this older post: Thanks Pat Zeitlow pzeitlow <@t> bbpllab.com Fri May 3 13:52:49 CDT 2013 Previous message: [Histonet] BRAF IHC Next message: [Histonet] BrDU Messages sorted by: [ date ] [ thread ] [ subject ] [ author ] Also using Spring Bioscience antibody. Trying to get it automated using Biocare instrument. You? Pat -----Original Message----- From: Bitting, Angela K. [mailto:akbitting <@t> geisinger.edu] Sent: Friday, May 03, 2013 1:45 PM To: Pat Zeitlow Subject: RE: BRAF IHC Working it up right now. Using Spring Bioscience's BRAF V600E. Are you using an automated platform? Angie -----Original Message----- From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Pat Zeitlow Sent: Friday, May 03, 2013 2:36 PM To: histonet <@t> lists.utsouthwestern.edu Subject: [Histonet] BRAF IHC Is anyone doing BRAF IHC staining that is willing to share protocol details? TIA, Pat From CThornton <@t> dahlchase.com Wed Jul 10 13:58:50 2013 From: CThornton <@t> dahlchase.com (Clare Thornton) Date: Wed Jul 10 13:58:54 2013 Subject: [Histonet] p40 Message-ID: Who is working with p40 antibody? Are you seeing any staining in (lung) adenocarcinomas? What about normal lung? Will p40 pick up a squamous differentiation in adenocarcinoma? Thanks for any help! Clare Clare J. Thornton, HTL(ASCP),QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com From dreynold <@t> mdanderson.org Wed Jul 10 17:09:31 2013 From: dreynold <@t> mdanderson.org (Reynolds,Donna M) Date: Wed Jul 10 17:09:41 2013 Subject: [Histonet] RE: Plexiglas humidity chambers Message-ID: <01C7405EC5F26E40A9A64015F13B461C284D37@D1PWPEXMBX04.mdanderson.edu> We are a research lab and still do all our staining by hand. Just prefer it that way. Our carpenter shop makes ours. We determine size by how many slides we want to stain at one time. We have chambers that will hold 5, 30, 40, 60 slides. We make the Plexiglas box out of black Plexiglas so it can be used for fluorescent or DAB labeling. Chambers are 2 inches deep, 12 ? " wide and length determined by how many slides we want it to hold. A piece of plexi 1" wide is glued to bottom standing on its side 1 inches from the side of chamber and ? " from the end of chamber. Then a lid is made with a small ridge of plexi on edges so that it will fit inside the bottom of the chamber to holds it in place when moving chamber. Then we take a thinner Plexiglas 2 ? inch wide and a little shorter than the internal width of chamber, glue a ? inch wide plexi to one side of it. Slides can then be taped to the plexi "stick" at the label end of slide with a good strong tape. Our "sticks" will hold 10 slides across them. When you are washing you can pick up the "stick" with the slides on it and wash 1 to 10 slides at a time. Hope this makes sense. We love this system for doing our IHC by hand. Donna Reynolds HT (ASCP) Chief Histo Tech Core IHC Lab Dept. Cancer Biology, 713-792-8106 From sbaldwin <@t> mhhcc.org Thu Jul 11 07:23:08 2013 From: sbaldwin <@t> mhhcc.org (Sara Baldwin/mhhcc.org) Date: Thu Jul 11 07:24:07 2013 Subject: [Histonet] CAP REG Message-ID: Is there a CAP or some Regulation with a percentage for correlating the frozen section diagnosis with the final diagnosis, my pathologist seems to think there is we are joint commision and can't find anything about this, can anyone help?? Thanks Histology/Cytology Supervisor S. Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph 812-996-0210, 0216, Fax 812-996-0232, Pager 812-481-0897, Cell 812-887-3357 From Wanda.Smith <@t> HCAhealthcare.com Thu Jul 11 08:01:53 2013 From: Wanda.Smith <@t> HCAhealthcare.com (Wanda.Smith@HCAhealthcare.com) Date: Thu Jul 11 08:01:59 2013 Subject: [Histonet] CAP REG In-Reply-To: References: Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BA27DC8F9C74@NADCWPMSGCMS03.hca.corpad.net> Good Morning to All, This is a CAP reg ANP.10100 Intra-operative/Final Diagnosis Disparity. I have a report that prints FS and final dx and I go through and check it each quarter for PI. If there is a discrepancy, I have a form the Pathologist fills out stating what the issue may have been, for example, Sectioning problem, Sampling problem, Inappropriate selection of material for FS, etc. The Pathologist checks one and I file it with my PI. Hope this helps. Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC? 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sara Baldwin/mhhcc.org Sent: Thursday, July 11, 2013 8:23 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP REG Is there a CAP or some Regulation with a percentage for correlating the frozen section diagnosis with the final diagnosis, my pathologist seems to think there is we are joint commision and can't find anything about this, can anyone help?? Thanks Histology/Cytology Supervisor S. Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph 812-996-0210, 0216, Fax 812-996-0232, Pager 812-481-0897, Cell 812-887-3357 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From m_chadwell <@t> hotmail.com Thu Jul 11 10:17:00 2013 From: m_chadwell <@t> hotmail.com (margaret chadwell) Date: Thu Jul 11 10:24:29 2013 Subject: [Histonet] Cutting Frozen Brain Message-ID: Hello Everyone, Does anyone have any suggestions for cutting corneal rat brains (20 microns), fixed in PFA, treated with sucrose and embedded into OCT. They cut well but when I pick them up on the slide they always have wrinkles, I have tried many different things with no luck. Different modes of rolling the tissue onto the slide Cold slides in the cryostat, placing the tissue on the slide, gently brushing it while warming from below with my finger And just dropping the slide onto the cut flattened section I am totally frustrated and need to find a way to improve the section quality at this point. Any suggestions or advise would be greatly appreciated! Thank you in advance! Margie Chadwell Sent from Windows Mail From mward <@t> wakehealth.edu Thu Jul 11 13:48:45 2013 From: mward <@t> wakehealth.edu (Martha Ward-Pathology) Date: Thu Jul 11 13:48:53 2013 Subject: [Histonet] DAB enhancer In-Reply-To: <004901ce7d8f$8e96b5c0$abc42140$@com> References: <004301ce7d89$fa2344d0$ee69ce70$@com> <64DB27005E2FD3439E88502D7A5C9121010241E276C2@CORTEZ.ucdenver.pvt> <004901ce7d8f$8e96b5c0$abc42140$@com> Message-ID: I have been asked by a new pathologist to look into a DAB enhancer for use on our Bond III instruments. Are many of you using this on the Bond, or any other staining platform and what are the pros and cons of its use? Thanks! ? Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard ?\? Winston-Salem, NC 27157 p 336.716.2109 ?\? f 336.716.5890 ? mward@wakehealth.edu ? ? ? Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mward <@t> wakehealth.edu Thu Jul 11 13:52:36 2013 From: mward <@t> wakehealth.edu (Martha Ward-Pathology) Date: Thu Jul 11 13:52:43 2013 Subject: [Histonet] DAB enhancer In-Reply-To: <0B8979A204680A42B93A52B486088CD938E883CB5D@CUAEXH1.GCU-MD.local> References: <493436627.1040751.1373306890387.JavaMail.root@sz0051a.emeryville.ca.mail.comcast.net>, <722423942.1040847.1373307022705.JavaMail.root@sz0051a.emeryville.ca.mail.comcast.net> <0B8979A204680A42B93A52B486088CD938E883CB5D@CUAEXH1.GCU-MD.local> Message-ID: Sorry but I cannot get my messages to go through to the Histonet so I am piggybacking onto a previous message: I have been asked by a new pathologist to look into a DAB enhancer for use on our Bond III instruments. Are many of you using this on the Bond, or any other staining platform and what are the pros and cons of its use? Thanks! Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mward@wakehealth.edu -----Original Message----- From Pat.Bell <@t> ucdenver.edu Thu Jul 11 14:10:32 2013 From: Pat.Bell <@t> ucdenver.edu (Bell, Pat) Date: Thu Jul 11 14:10:36 2013 Subject: [Histonet] iNOS Message-ID: <64DB27005E2FD3439E88502D7A5C9121010241E27C55@CORTEZ.ucdenver.pvt> Hello to Everyone, Does anyone have an antibody for iNOS that will work in Human? I appreciate any help you wonderful people could give me. Also, could you share your protocol with me? Thanks, Pat Pat Bell HT(ASCP) Medical Oncology University of Colorado, Denver 12801 E 17th Ave, MS 8117 Aurora, Colorado 80045 303-724-6077 pat.bell@ucdenver.edu From Rcartun <@t> harthosp.org Thu Jul 11 14:46:29 2013 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Thu Jul 11 14:46:40 2013 Subject: [Histonet] DAB enhancer In-Reply-To: References: <004301ce7d89$fa2344d0$ee69ce70$@com> <64DB27005E2FD3439E88502D7A5C9121010241E276C2@CORTEZ.ucdenver.pvt> <004901ce7d8f$8e96b5c0$abc42140$@com> Message-ID: <51DED355020000770003CFBC@gwmail3.harthosp.org> I don't know why anyone would want to use a DAB enhancer for slides prepared on Leica-Microsystems' Bond Max platforms. The resulting immunoreactivity is exceptionally "Robust" in my experience when using their Bond Polymer Refine Detection kit. Could this request be for a protein target expressed at low levels? Is your hematoxylin too dark obscuring the immunoreactivity? Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> Martha Ward-Pathology 7/11/2013 2:48 PM >>> I have been asked by a new pathologist to look into a DAB enhancer for use on our Bond III instruments. Are many of you using this on the Bond, or any other staining platform and what are the pros and cons of its use? Thanks! Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mward@wakehealth.edu Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From POWELL_SA <@t> mercer.edu Thu Jul 11 16:02:13 2013 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Thu Jul 11 16:02:17 2013 Subject: [Histonet] Ciliated Protozoa Message-ID: <9BF995BC0E47744E9673A41486E24EE2583F686BE2@MERCERMAIL.MercerU.local> I am way out of my league here and need help from the histonet world. I have been asked to do wet and dry silver nitrate-protargol staining for a research project on ciliated protozoa. I need to speak with someone out there who has done this before. You may contact me directly. Thanks Shirley A. Powell, HT(ASCP)HTL, QIHC Technical Director Histology Curricular Support Laboratory Mercer University School of Medicine 1550 College Street Macon, GA 31207 478-301-2374 Lab 478-301-5489 Fax From Steven.Weston <@t> utas.edu.au Thu Jul 11 19:31:07 2013 From: Steven.Weston <@t> utas.edu.au (Steven Weston) Date: Thu Jul 11 19:31:18 2013 Subject: [Histonet] re wrinkles in section Message-ID: Hi Margaret, One possible solution to the wrinkling problem you have is to float your twenty microns onto the slide. I do this regularly with mouse or rat brain by cutting the section and putting it into culture wells with buffer solution. Then once I have completed my sectioning I lift the individual sections using a hockey stick made from a bent glass pasteur pipette (although a good fine paintbrush works as well) and dip them into a 30% ethanol solution briefly and then onto a distilled water bath. When you lower the section onto the water bath (at room temp) it should spread due to surface tension changes, and then you can pick it up onto a charged slide as you would a paraffin section. You then allow the section to dry on the slide. This also removes the OCT and gives you nice sections on clean slide that, once dried, stain well. (I usually dry in a 37 degree incubator overnight). Hope this helps Steve Weston Lab Manager Breathe-Well Centre of Research Excellence for Chronic Respiratory Disease. UTAS-SOM 0408990859 62264871 From rgeske_2000 <@t> yahoo.com Fri Jul 12 05:20:15 2013 From: rgeske_2000 <@t> yahoo.com (Rob Geske) Date: Fri Jul 12 06:15:31 2013 Subject: [Histonet] Hamamatsu NanoZoomer In-Reply-To: <51DED355020000770003CFBC@gwmail3.harthosp.org> References: <004301ce7d89$fa2344d0$ee69ce70$@com> <64DB27005E2FD3439E88502D7A5C9121010241E276C2@CORTEZ.ucdenver.pvt> <004901ce7d8f$8e96b5c0$abc42140$@com> <51DED355020000770003CFBC@gwmail3.harthosp.org> Message-ID: <1373624415.83535.YahooMailNeo@web160501.mail.bf1.yahoo.com> All, i would appreciate any user experience with the latest version of Hamamatsu's digital slide scanner (NanoZoomer 2.0-HT C9600-13) that is fitted with the fluorescence imaging module -- link below.? We've had our NanoZoomer? (with fluorescence) for? over 7 years and it has been reliable and generates excellent quality digital scans both bright field and fluorescent.? i'm considering? either upgrading the fluorescent capability in the current unit or doing a full instrument replacement and actual user comments will be very useful. we will be sending Hamamatsu some of our slides for scanning as well as hopefully getting some hands on experience with the instrument.? thanks in advance. regards, rob http://www.hamamatsu.com/us/en/product/category/5002/5007/C9600-13/index.html From d-emge <@t> northwestern.edu Fri Jul 12 07:47:14 2013 From: d-emge <@t> northwestern.edu (Donna J Emge) Date: Fri Jul 12 07:47:22 2013 Subject: [Histonet] Cutting Frozen Brain Message-ID: <54C3042A8564BA4AA2D355DD1D4C949C11AE28DC@CHCSPMBX1.ads.northwestern.edu> The wrinkling can happen sometimes when cutting 4%PFA, sucrose cryoprotected brain samples. Flat sections that wrinkle when picked up on the slide. Using a soft brush, moisten it with distilled water and swipe it across the area of pickup on your slide, then pick up your section. It will flatten. Works like a charm! You do not need the brush too wet, so shake off the excess water before swiping the slide. Also, if doing insitu hybridization use inactivated DEPC or RNase free water using standard clean techniques for the brush between samples. Donna J. Emge, HT-ASCP Mouse Histology and Phenotyping Core Manager Northwestern University Olson Building room 8-333 710 North Fairbanks Court Chicago, IL 60611 MHPL@northwestern.edu d-emge@northwestern.edu Lab 312-503-2679 Fax 312-503-2369 From amber.mckenzie <@t> gastrodocs.net Fri Jul 12 08:33:20 2013 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Fri Jul 12 08:33:23 2013 Subject: [Histonet] Label identifiers In-Reply-To: References: Message-ID: <5A33C952BB67F4468AF1F36D739212BCBD6B9E8F@JERRY.Gia.com> I'm trying to get our Co-path computer system set up to print labels instead of us handwriting on the paperwork and containers...what information do you guys have on your printed labels...just patient name and accession no? Thanks! From Joyce.Weems <@t> EmoryHealthcare.org Fri Jul 12 09:07:06 2013 From: Joyce.Weems <@t> EmoryHealthcare.org (Weems, Joyce K.) Date: Fri Jul 12 09:07:45 2013 Subject: [Histonet] RE: Label identifiers In-Reply-To: <5A33C952BB67F4468AF1F36D739212BCBD6B9E8F@JERRY.Gia.com> References: <5A33C952BB67F4468AF1F36D739212BCBD6B9E8F@JERRY.Gia.com> Message-ID: Patient names, case number, hospital name, city and state, and a bar code here Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph?s Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Friday, July 12, 2013 9:33 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Label identifiers I'm trying to get our Co-path computer system set up to print labels instead of us handwriting on the paperwork and containers...what information do you guys have on your printed labels...just patient name and accession no? Thanks! ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From o.m.gallagher <@t> sheffield.ac.uk Fri Jul 12 09:43:23 2013 From: o.m.gallagher <@t> sheffield.ac.uk (Orla M Gallagher) Date: Fri Jul 12 09:43:29 2013 Subject: [Histonet] How to remove 80% ethanol from bones before decalcifying in EDTA Message-ID: Dear Histonetters, I have some mouse bones samples which have been fixed in 70% ethanol for a few weeks and stored in 80% ethanol for a week; now our colleague would like to decalcify them in EDTA for wax embedding and staining. What is the best way to remove the ethanol before transfer to EDTA? Thanks, Orla -- ************************** Ms. Orla Gallagher Bone Analysis Laboratory Mellanby Centre for Bone Research Department of Human Metabolism D Floor Medical School University of Sheffield Beech Hill Road Sheffield S10 2RX UK Website: http://mellanbycentre.dept.shef.ac.uk Tel: 0044114-2713337 (office) 0044114-2713174 (lab) E-Mail: o.m.gallagher@sheffield.ac.uk STOP: Do you really need to print this e-mail? BE GREEN: Keep it on the screen. Times Higher Education University of the Year Data protection and confidentiality: The information contained in this message or any appended documents may be privileged and confidential and is intended for the exclusive use of the addressee(s). If you are not the addressee, any disclosure, reproduction, distributions, other dissemination or use of this message is strictly prohibited and may be unlawful. If you receive this correspondence in error please contact the sender immediately and permanently delete/destroy what you have received. From o.m.gallagher <@t> sheffield.ac.uk Fri Jul 12 09:52:03 2013 From: o.m.gallagher <@t> sheffield.ac.uk (Orla M Gallagher) Date: Fri Jul 12 09:52:11 2013 Subject: [Histonet] Bone decalcifying/processing problem Message-ID: Dear Histonetters, Has anyone ever seen large holes/vacuoles in the bone marrow of mouse long bones after decalcification and processing to wax? We've had this problem with a study which was fixed in 4% paraformaldehyde and decalcified at 4 degrees C over a 14 day period according to a routinely-used protocol involving changes of 15% EDTA/0.5% PFA in PBS pH 8.0, rinsed in PBS to remove EDTA before processing to paraffin wax. The marrow is filled with large holes, almost like balloons. I'm happy to send images to anyone who might be able to help! Best wishes, Orla -- ************************** Ms. Orla Gallagher Bone Analysis Laboratory Mellanby Centre for Bone Research Department of Human Metabolism D Floor Medical School University of Sheffield Beech Hill Road Sheffield S10 2RX UK Website: http://mellanbycentre.dept.shef.ac.uk Tel: 0044114-2713337 (office) 0044114-2713174 (lab) E-Mail: o.m.gallagher@sheffield.ac.uk STOP: Do you really need to print this e-mail? BE GREEN: Keep it on the screen. Times Higher Education University of the Year Data protection and confidentiality: The information contained in this message or any appended documents may be privileged and confidential and is intended for the exclusive use of the addressee(s). If you are not the addressee, any disclosure, reproduction, distributions, other dissemination or use of this message is strictly prohibited and may be unlawful. If you receive this correspondence in error please contact the sender immediately and permanently delete/destroy what you have received. From liz <@t> premierlab.com Fri Jul 12 09:55:38 2013 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Fri Jul 12 09:58:30 2013 Subject: [Histonet] Bone decalcifying/processing problem In-Reply-To: References: Message-ID: <14E2C6176416974295479C64A11CB9AE016411AB4A3A@SBS2K8.premierlab.local> Orla I know this might sound silly but normal mouse bone marrow contains both fat and hemoatopoietic marrow, the areas that are fat will appear like round voids. Is that what you are seeing? Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 881-0763 cell (303) 682-9060 fax liz@premierlab.com Ship to address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Orla M Gallagher [o.m.gallagher@sheffield.ac.uk] Sent: Friday, July 12, 2013 8:52 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Bone decalcifying/processing problem Dear Histonetters, Has anyone ever seen large holes/vacuoles in the bone marrow of mouse long bones after decalcification and processing to wax? We've had this problem with a study which was fixed in 4% paraformaldehyde and decalcified at 4 degrees C over a 14 day period according to a routinely-used protocol involving changes of 15% EDTA/0.5% PFA in PBS pH 8.0, rinsed in PBS to remove EDTA before processing to paraffin wax. The marrow is filled with large holes, almost like balloons. I'm happy to send images to anyone who might be able to help! Best wishes, Orla -- ************************** Ms. Orla Gallagher Bone Analysis Laboratory Mellanby Centre for Bone Research Department of Human Metabolism D Floor Medical School University of Sheffield Beech Hill Road Sheffield S10 2RX UK Website: http://mellanbycentre.dept.shef.ac.uk Tel: 0044114-2713337 (office) 0044114-2713174 (lab) E-Mail: o.m.gallagher@sheffield.ac.uk STOP: Do you really need to print this e-mail? BE GREEN: Keep it on the screen. Times Higher Education University of the Year Data protection and confidentiality: The information contained in this message or any appended documents may be privileged and confidential and is intended for the exclusive use of the addressee(s). If you are not the addressee, any disclosure, reproduction, distributions, other dissemination or use of this message is strictly prohibited and may be unlawful. If you receive this correspondence in error please contact the sender immediately and permanently delete/destroy what you have received. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TMcNemar <@t> lmhealth.org Fri Jul 12 10:13:49 2013 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Fri Jul 12 10:13:46 2013 Subject: [Histonet] RE: Label identifiers In-Reply-To: <5A33C952BB67F4468AF1F36D739212BCBD6B9E8F@JERRY.Gia.com> References: <5A33C952BB67F4468AF1F36D739212BCBD6B9E8F@JERRY.Gia.com> Message-ID: We have patient name, accession number, the pathologist's initials, hospital name and address, and special stain, or H&E, or level, etc. The accession number and DOB is also included in a barcode that is printed on the label. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Friday, July 12, 2013 9:33 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Label identifiers I'm trying to get our Co-path computer system set up to print labels instead of us handwriting on the paperwork and containers...what information do you guys have on your printed labels...just patient name and accession no? Thanks! This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From ree3 <@t> leicester.ac.uk Fri Jul 12 10:17:48 2013 From: ree3 <@t> leicester.ac.uk (Edwards, Richard E.) Date: Fri Jul 12 10:17:54 2013 Subject: [Histonet] tol blue and mast cells Message-ID: <7722595275A4DD4FA225B92CDBF174A101B0D08CC273@EXC-MBX3.cfs.le.ac.uk> Hi All, your thoughts on the best toluidine blue method for mast cells?, many thanks. Richard Edwards University of Leicester U.K. From TMcNemar <@t> lmhealth.org Fri Jul 12 10:22:54 2013 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Fri Jul 12 10:22:46 2013 Subject: [Histonet] RE: Label identifiers In-Reply-To: References: <5A33C952BB67F4468AF1F36D739212BCBD6B9E8F@JERRY.Gia.com> Message-ID: Whoops! I guess you probably weren?t asking about slide labels. For container labels, it is barcode, patient name, account number, specimen source, and container number. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Friday, July 12, 2013 11:14 AM To: 'Amber McKenzie'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Label identifiers We have patient name, accession number, the pathologist's initials, hospital name and address, and special stain, or H&E, or level, etc. The accession number and DOB is also included in a barcode that is printed on the label. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Friday, July 12, 2013 9:33 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Label identifiers I'm trying to get our Co-path computer system set up to print labels instead of us handwriting on the paperwork and containers...what information do you guys have on your printed labels...just patient name and accession no? Thanks! This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From liz <@t> premierlab.com Fri Jul 12 10:21:50 2013 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Fri Jul 12 10:25:29 2013 Subject: [Histonet] RE: tol blue and mast cells In-Reply-To: <7722595275A4DD4FA225B92CDBF174A101B0D08CC273@EXC-MBX3.cfs.le.ac.uk> References: <7722595275A4DD4FA225B92CDBF174A101B0D08CC273@EXC-MBX3.cfs.le.ac.uk> Message-ID: <14E2C6176416974295479C64A11CB9AE016411AB4A3B@SBS2K8.premierlab.local> Richard We use 0.04% T. Blue in an acetate buffer that is pH'd to 4.0. Stain for about 5 minutes, rinse in water, let air dry and then coverslip in xylene. You will loose the metachromatic staining if you run the slides through alcohol. I have an SOP if you would like to see one. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 881-0763 cell (303) 682-9060 fax liz@premierlab.com Ship to address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Edwards, Richard E. [ree3@leicester.ac.uk] Sent: Friday, July 12, 2013 9:17 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] tol blue and mast cells Hi All, your thoughts on the best toluidine blue method for mast cells?, many thanks. Richard Edwards University of Leicester U.K. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From llewllew <@t> shaw.ca Fri Jul 12 10:39:54 2013 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Fri Jul 12 10:40:08 2013 Subject: [Histonet] tol blue and mast cells In-Reply-To: <7722595275A4DD4FA225B92CDBF174A101B0D08CC273@EXC-MBX3.cfs.le.ac.uk> References: <7722595275A4DD4FA225B92CDBF174A101B0D08CC273@EXC-MBX3.cfs.le.ac.uk> Message-ID: <51E0234A.2070708@shaw.ca> Try the one on stainsfile (http://stainsfile.info/StainsFile/stain/cell/aldtolblue.htm). It gives dark blue mast cell granules and is not removed with ethanol dehydration. Bryan Llewellyn Edwards, Richard E. wrote: > > Hi All, your thoughts on the best toluidine blue method for mast cells?, many thanks. > > > Richard Edwards > > University of Leicester > > U.K. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From BDeBrosse-Serra <@t> isisph.com Fri Jul 12 11:39:26 2013 From: BDeBrosse-Serra <@t> isisph.com (Bea DeBrosse-Serra) Date: Fri Jul 12 11:39:34 2013 Subject: [Histonet] Contract laboratories for 2 x 3 brain sections; cutting, staining, possibly scanning Message-ID: <493CAA64F203E14E8823737B9EE0E25F09488DD8C7@EXCHMB01.isis.local> Hello histonetters, Who do you use for large brain slides (monkey, pig) to do the studies? Contract labs are welcome to contact me too. Thanks, Bea Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 From arvidsonkristen <@t> yahoo.com Fri Jul 12 12:25:17 2013 From: arvidsonkristen <@t> yahoo.com (kristen arvidson) Date: Fri Jul 12 12:25:27 2013 Subject: [Histonet] Eosin Staining Issues Message-ID: <1373649917.92619.YahooMailNeo@web122204.mail.ne1.yahoo.com> Hello All! ? I am having some bizzare staining issues.? The eosin seems to be washing out??? The slides look "sun bleached" according to the Pathologists.? The problem is that it is only happening on occasion.?? One rack stains fine and the next one has a problem.? ?It even appears to be ?happening within cases staining in the same rack.? My first though is tap water ph.? We don't monitor that, haven't needed to, ?we've?never had a problem like this before.? Thoughts??? Thanks From estelahisto <@t> gmail.com Fri Jul 12 12:47:11 2013 From: estelahisto <@t> gmail.com (Estela Molina) Date: Fri Jul 12 12:47:14 2013 Subject: [Histonet] Adipose tissue engineering Message-ID: What is the best technique th process scaffolds? we are using adipose tissue engineering with an alginate/gelating scaffold. Thanks From Pathrm35 <@t> comcast.net Fri Jul 12 13:11:37 2013 From: Pathrm35 <@t> comcast.net (Pathrm35@comcast.net) Date: Fri Jul 12 13:12:04 2013 Subject: [Histonet] 88363 cpt code Message-ID: <85802262.1438611.1373652697271.JavaMail.root@sz0123a.westchester.pa.mail.comcast.net> I was wondering if anyone bills insurance companies for a 88363 cpt code? This is for retrieving archived blocks and slides, packaging them and sending them out to outside facilities for molecular testing (PTEN, KRAS,etc). If you do bill, what is the charge? ? Thanks, Ron From Joyce.Weems <@t> emoryhealthcare.org Fri Jul 12 13:13:17 2013 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Fri Jul 12 13:13:25 2013 Subject: [Histonet] 88363 cpt code In-Reply-To: <85802262.1438611.1373652697271.JavaMail.root@sz0123a.westchester.pa.mail.comcast.net> References: <85802262.1438611.1373652697271.JavaMail.root@sz0123a.westchester.pa.mail.comcast.net> Message-ID: Yes, we do - $25.00 or so.. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph?s Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pathrm35@comcast.net Sent: Friday, July 12, 2013 2:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] 88363 cpt code I was wondering if anyone bills insurance companies for a 88363 cpt code? This is for retrieving archived blocks and slides, packaging them and sending them out to outside facilities for molecular testing (PTEN, KRAS,etc). If you do bill, what is the charge? Thanks, Ron _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From Joyce.Weems <@t> emoryhealthcare.org Fri Jul 12 13:14:06 2013 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Fri Jul 12 13:14:14 2013 Subject: [Histonet] 88363 cpt code In-Reply-To: <85802262.1438611.1373652697271.JavaMail.root@sz0123a.westchester.pa.mail.comcast.net> References: <85802262.1438611.1373652697271.JavaMail.root@sz0123a.westchester.pa.mail.comcast.net> Message-ID: And I should clarify - that is the technical part. We bill separately from the pathologists. I don't know what their price is. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph?s Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pathrm35@comcast.net Sent: Friday, July 12, 2013 2:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] 88363 cpt code I was wondering if anyone bills insurance companies for a 88363 cpt code? This is for retrieving archived blocks and slides, packaging them and sending them out to outside facilities for molecular testing (PTEN, KRAS,etc). If you do bill, what is the charge? Thanks, Ron _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From John.McGinley <@t> ColoState.EDU Fri Jul 12 13:40:13 2013 From: John.McGinley <@t> ColoState.EDU (McGinley,John) Date: Fri Jul 12 13:40:20 2013 Subject: [Histonet] Aperio ScanScope Message-ID: <9633BECFEA62114E8318B7754A08866C0253F216@ex13.colostate.edu> Hi, I realize this is a longshot, but the research principal investigator I work for asked me to inquire. Does anyone have an Aperio ScanScope they would consider donating or selling at a greatly reduced price? Thanks, John ----------------------------- John N. McGinley Cancer Prevention Laboratory Colorado State University 1173 Campus Delivery Fort Collins, CO 80523-1173 john.mcginley@colostate.edu www.cpl.colostate.edu From gu.lang <@t> gmx.at Fri Jul 12 13:43:18 2013 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Fri Jul 12 13:43:20 2013 Subject: AW: [Histonet] Eosin Staining Issues In-Reply-To: <1373649917.92619.YahooMailNeo@web122204.mail.ne1.yahoo.com> References: <1373649917.92619.YahooMailNeo@web122204.mail.ne1.yahoo.com> Message-ID: <000c01ce7f2f$ad22c2d0$07684870$@gmx.at> What about section-thickness? Do you have a tech, who cuts very thin? I have sometimes this problem, that the staining looks very faint because the slide is just too thin. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von kristen arvidson Gesendet: Freitag, 12. Juli 2013 19:25 An: histonet Betreff: [Histonet] Eosin Staining Issues Hello All! ? I am having some bizzare staining issues.? The eosin seems to be washing out??? The slides look "sun bleached" according to the Pathologists.? The problem is that it is only happening on occasion.?? One rack stains fine and the next one has a problem.? ?It even appears to be ?happening within cases staining in the same rack.? My first though is tap water ph.? We don't monitor that, haven't needed to, ?we've?never had a problem like this before.? Thoughts??? Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Fri Jul 12 14:01:49 2013 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Fri Jul 12 14:03:42 2013 Subject: [Histonet] RE: Aperio ScanScope In-Reply-To: <9633BECFEA62114E8318B7754A08866C0253F216@ex13.colostate.edu> References: <9633BECFEA62114E8318B7754A08866C0253F216@ex13.colostate.edu> Message-ID: <14E2C6176416974295479C64A11CB9AE016411AB4A4E@SBS2K8.premierlab.local> John There are plenty labs out there that will scan slides as a fee for service we do that for CSU already for the microbiology department. There are also some less expensive scanners out there that scan one to five slides at a time that would be less expensive than an Aperio Scan Scope Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 881-0763 cell (303) 682-9060 fax liz@premierlab.com Ship to address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 ________________________________________ From: ihcrg@googlegroups.com [ihcrg@googlegroups.com] On Behalf Of McGinley,John [John.McGinley@ColoState.EDU] Sent: Friday, July 12, 2013 12:40 PM To: histonet@lists.utsouthwestern.edu; ihcrg@googlegroups.com Subject: [IHCRG] Aperio ScanScope Hi, I realize this is a longshot, but the research principal investigator I work for asked me to inquire. Does anyone have an Aperio ScanScope they would consider donating or selling at a greatly reduced price? Thanks, John ----------------------------- John N. McGinley Cancer Prevention Laboratory Colorado State University 1173 Campus Delivery Fort Collins, CO 80523-1173 john.mcginley@colostate.edu www.cpl.colostate.edu -- -- You received this message because you are subscribed to the Google Groups "ihcrg" group. The IHC Resource Group is a standing committee within the National Society for Histotechnology. To post to this group, send email to ihcrg@googlegroups.com To unsubscribe from this group, send email to ihcrg+unsubscribe@googlegroups.com For more options, visit this group at http://groups.google.com/group/ihcrg?hl=en To contact the National Society for Histotechnology, email: histo@nsh.org or call 443.535.4060. --- You received this message because you are subscribed to the Google Groups "ihcrg" group. To unsubscribe from this group and stop receiving emails from it, send an email to ihcrg+unsubscribe@googlegroups.com. For more options, visit https://groups.google.com/groups/opt_out. From jhabecke <@t> fhcrc.org Fri Jul 12 14:30:11 2013 From: jhabecke <@t> fhcrc.org (Randolph-Habecker, Julie) Date: Fri Jul 12 14:30:18 2013 Subject: [Histonet] Anti-Human Hepatocyte antibody clone OCH1E5 Message-ID: Hey Histonetters, Has anyone noted negative staining with the anti-human hepatocyte antibody Clone OCH1E5 on hepatocytes surrounding the centrilobular vein? We are staining mouse liver samples and get great staining on about 80-90% of the hepatocytes in normal liver but then see very low to frank negative staining only in this region. Thanks! Julie Julie Randolph-Habecker, Ph.D. Director, Experimental Histopathology Shared Resources Fred Hutchinson Cancer Research Center 1100 Fairview Ave N, DE-360 Seattle WA 98109-1024 Tel: 206-667-6119 Fax: 206-667-6845 jhabecke@fhcrc.org From Rcartun <@t> harthosp.org Fri Jul 12 17:22:51 2013 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Jul 12 17:23:03 2013 Subject: [Histonet] 88363 cpt code In-Reply-To: <85802262.1438611.1373652697271.JavaMail.root@sz0123a.westchester.pa.mail.comcast.net> References: <85802262.1438611.1373652697271.JavaMail.root@sz0123a.westchester.pa.mail.comcast.net> Message-ID: <51E0497B020000770003D0FF@gwmail3.harthosp.org> Is there a "Technical" charge (TC) for 88369 or is it only a "Professional" charge that the pathologist bills? Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> 7/12/2013 2:11 PM >>> I was wondering if anyone bills insurance companies for a 88363 cpt code? This is for retrieving archived blocks and slides, packaging them and sending them out to outside facilities for molecular testing (PTEN, KRAS,etc). If you do bill, what is the charge? Thanks, Ron _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Sat Jul 13 05:41:07 2013 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Sat Jul 13 05:41:12 2013 Subject: [Histonet] DAB enhancer In-Reply-To: References: <493436627.1040751.1373306890387.JavaMail.root@sz0051a.emeryville.ca.mail.comcast.net>, , <722423942.1040847.1373307022705.JavaMail.root@sz0051a.emeryville.ca.mail.comcast.net>, <0B8979A204680A42B93A52B486088CD938E883CB5D@CUAEXH1.GCU-MD.local>, Message-ID: So far, in my situation, there has been no need. I would suggest maybe looking at the overall optimization, and maybe contacting a Leica field representative. They have information about what has been working for others and this may streamline the troubleshooting. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: mward@wakehealth.edu > To: wbenton@cua.md; davidclark10@comcast.net; histonet@lists.utsouthwestern.edu > Date: Thu, 11 Jul 2013 18:52:36 +0000 > CC: > Subject: [Histonet] DAB enhancer > > Sorry but I cannot get my messages to go through to the Histonet so I am piggybacking onto a previous message: > > > I have been asked by a new pathologist to look into a DAB enhancer for use on our Bond III instruments. Are many of you using this on the Bond, or any other staining platform and what are the pros and cons of its use? > > Thanks! > > > Martha Ward, MT (ASCP) QIHC > Manager > > Molecular Diagnostics Lab > Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mward@wakehealth.edu > > -----Original Message----- > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Sat Jul 13 05:42:25 2013 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Sat Jul 13 05:42:29 2013 Subject: [Histonet] 88363 cpt code In-Reply-To: <85802262.1438611.1373652697271.JavaMail.root@sz0123a.westchester.pa.mail.comcast.net> References: <85802262.1438611.1373652697271.JavaMail.root@sz0123a.westchester.pa.mail.comcast.net> Message-ID: Yes, since my facility is primarily molecular. I can look up the reimbursement from my spreadsheet next week if you need that ( at home now). Joelle Weaver MAOM, HTL (ASCP) QIHC > Date: Fri, 12 Jul 2013 18:11:37 +0000 > From: Pathrm35@comcast.net > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] 88363 cpt code > > > > > I was wondering if anyone bills insurance companies for a 88363 cpt code? This is for retrieving archived blocks and slides, packaging them and sending them out to outside facilities for molecular testing (PTEN, KRAS,etc). If you do bill, what is the charge? > > > > Thanks, > > Ron > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Sat Jul 13 05:44:57 2013 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Sat Jul 13 05:45:01 2013 Subject: [Histonet] 88363 cpt code In-Reply-To: <51E0497B020000770003D0FF@gwmail3.harthosp.org> References: <85802262.1438611.1373652697271.JavaMail.root@sz0123a.westchester.pa.mail.comcast.net>, <51E0497B020000770003D0FF@gwmail3.harthosp.org> Message-ID: By my recollection of the cpt resource, it depends on if the material needs to have additional slides or tissue preparation prepared in that facility. If so, I believe that you have some TC charges. I can do a double check on that for all. Joelle Weaver MAOM, HTL (ASCP) QIHC Date: Fri, 12 Jul 2013 18:22:51 -0400 From: Rcartun@harthosp.org To: Pathrm35@comcast.net; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] 88363 cpt code CC: Is there a "Technical" charge (TC) for 88369 or is it only a "Professional" charge that the pathologist bills? Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> 7/12/2013 2:11 PM >>> I was wondering if anyone bills insurance companies for a 88363 cpt code? This is for retrieving archived blocks and slides, packaging them and sending them out to outside facilities for molecular testing (PTEN, KRAS,etc). If you do bill, what is the charge? Thanks, Ron _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tony.henwood <@t> health.nsw.gov.au Sun Jul 14 18:16:31 2013 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Sun Jul 14 18:16:45 2013 Subject: [Histonet] Bone decalcifying/processing problem In-Reply-To: References: Message-ID: <6D6BD1DE8A5571489398B392A38A71579D28BB9A@xmdb04.nch.kids> Orla, You seem to have introduced many poor histologic processing practices into your technique. Firstly ???4% paraformaldehyde. How did you make this up? The nomenclature is incorrect (see Histonet archives and Manoonkitiwongsa PS, Schultz RL. (2002) "Proper nomenclature of formaldehyde and paraformaldehyde fixatives for Histochemistry" Histochem J 34: 365-367). Secondly, why fix and decal at 4oC. Low temperatures slow down the rate of diffusion and therefore fixation and can cause the formation of ice crystal artefact, which seems like what you have here. Also if you are going to decalcify tissues then I would recommend doubling your fixation time. Remember one of the big advantages of aldehyde fixation is that it cross-links and thereby protects cell and tissue constituents from processing. Try doing it all at room temperature, I bet that the artefacts will disappear. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Orla M Gallagher Sent: Saturday, 13 July 2013 12:52 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Bone decalcifying/processing problem Dear Histonetters, Has anyone ever seen large holes/vacuoles in the bone marrow of mouse long bones after decalcification and processing to wax? We've had this problem with a study which was fixed in 4% paraformaldehyde and decalcified at 4 degrees C over a 14 day period according to a routinely-used protocol involving changes of 15% EDTA/0.5% PFA in PBS pH 8.0, rinsed in PBS to remove EDTA before processing to paraffin wax. The marrow is filled with large holes, almost like balloons. I'm happy to send images to anyone who might be able to help! Best wishes, Orla -- ************************** Ms. Orla Gallagher Bone Analysis Laboratory Mellanby Centre for Bone Research Department of Human Metabolism D Floor Medical School University of Sheffield Beech Hill Road Sheffield S10 2RX UK Website: http://mellanbycentre.dept.shef.ac.uk Tel: 0044114-2713337 (office) 0044114-2713174 (lab) E-Mail: o.m.gallagher@sheffield.ac.uk STOP: Do you really need to print this e-mail? BE GREEN: Keep it on the screen. Times Higher Education University of the Year Data protection and confidentiality: The information contained in this message or any appended documents may be privileged and confidential and is intended for the exclusive use of the addressee(s). If you are not the addressee, any disclosure, reproduction, distributions, other dissemination or use of this message is strictly prohibited and may be unlawful. If you receive this correspondence in error please contact the sender immediately and permanently delete/destroy what you have received. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From tony.henwood <@t> health.nsw.gov.au Sun Jul 14 18:19:49 2013 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Sun Jul 14 18:20:18 2013 Subject: [Histonet] RE: tol blue and mast cells In-Reply-To: <7722595275A4DD4FA225B92CDBF174A101B0D08CC273@EXC-MBX3.cfs.le.ac.uk> References: <7722595275A4DD4FA225B92CDBF174A101B0D08CC273@EXC-MBX3.cfs.le.ac.uk> Message-ID: <6D6BD1DE8A5571489398B392A38A71579D28BBAC@xmdb04.nch.kids> Hi Richard, I would strongly recommend the Churukian and Schenk method (J.Histotechnol 4(2):85-86, 1981). A simple but brilliant stain. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Edwards, Richard E. Sent: Saturday, 13 July 2013 1:18 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] tol blue and mast cells Hi All, your thoughts on the best toluidine blue method for mast cells?, many thanks. Richard Edwards University of Leicester U.K. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From a.prior <@t> tissueregenix.com Mon Jul 15 02:33:25 2013 From: a.prior <@t> tissueregenix.com (Andrew Prior) Date: Mon Jul 15 02:31:28 2013 Subject: [Histonet] tol blue and mast cells Message-ID: I used this method in a previous job which gave good results on 5um sections of FFPE mouse lung. The eosin counterstain allowed easier navigation around the tissue and meant the tissue was dark enough for the slide scanner to "see" automatically. * Dewax and rehydrate to distilled water * Toluidine blue - 5 seconds * Running tap water - 20 seconds * 1% acetic acid - 1 second * Running tap water - 20 seconds * 0.5% Eosin - 1 second * Running tap water - 20 seconds * Ethanol - 45 seconds x3 * Xylene - 45 seconds x3 * Coverslip with DPX Andrew Prior Histologist Tissue Regenix Group E-mail: a.prior@tissueregenix.com Website: www.tissueregenix.com From ree3 <@t> leicester.ac.uk Mon Jul 15 04:55:24 2013 From: ree3 <@t> leicester.ac.uk (Edwards, Richard E.) Date: Mon Jul 15 04:55:31 2013 Subject: [Histonet] non-xylene dewaxing. Message-ID: <7722595275A4DD4FA225B92CDBF174A101B0D08CC282@EXC-MBX3.cfs.le.ac.uk> Hi All, any preferred methods?, all information gratefully received, many thanks. Richard Edwards University of Leicester U.K. From relia1 <@t> earthlink.net Mon Jul 15 08:32:39 2013 From: relia1 <@t> earthlink.net (Pam Barker) Date: Mon Jul 15 08:32:38 2013 Subject: [Histonet] RELIA Histology Careers Bulletin 7-15-2013 I hope you are having a great Summer!! Message-ID: <00b001ce815f$c74cf9f0$55e6edd0$@earthlink.net> Hi Histonetters! What are you doing on your summer vacation? Been anyplace you would like to move to? Closer to the beach or the mountains? Closer to family or friends? I can help!! There is still time this summer to make that move. I have opportunities in all kinds of labs in all areas of the country. Your next position is just a phone call or an e-mail away. All of the opportunities that I represent are permanent full time positions with companies that offer excellent salaries, benefits and relocation assistance. Here are the opportunities that I am most excited about: HISTOLOGY MANAGEMENT/SUPERVISORS Pathology Manager Vet/Research - Boston, MA Lead Histotechnologist - Long Beach, CA Lead Histotechnologist - near Columbus, OH Lead Histotechnologist - Ridgecrest, CA HISTOTECHNICIAN/HISTOTECHNOLOGIST Nashville,TN - cutting and embedding ASCP not required ***ASCP HT/HTL and 2 years exper required for all of these positions.*** Paterson, NJ-Night shift CLIA qual to gross Charlotte, NC-Day and night shifts 10K RELo/Sign On. Bristol, TN - Days private lab strong routine histology Tyler, TX - Days strong routine histology Augusta, GA - Days routine histology Ridgecrest, CA - Lead Histotech day Atlanta, GA - Multiple shifts ASCP HT/HTL required. Mohs Histotech - Ann Arbor, MI OTHER POSITIONS Molecular Diagnostics Tech Augusta, GA Cytogenetics Tech - Paterson, NJ If you or anyone you know are interested in hearing more about any of these opportunities or have another type of position or area in mind and would like some help in your job search please let me know. Just shoot me an e-mail at relia1@earthlink.net or give me a call toll free at 866-607-3542. Remember RELIA pays a referral fee if we place someone you refer to us! We appreciate the help and your friends do too!! Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From staceyjm <@t> sbcglobal.net Mon Jul 15 12:09:47 2013 From: staceyjm <@t> sbcglobal.net (STACEY) Date: Mon Jul 15 12:10:03 2013 Subject: [Histonet] (no subject) Message-ID: <1373908187.34444.YahooMailNeo@web184403.mail.bf1.yahoo.com> Our laboratory has a Leica Peloris processor. Currently, we are using unbuffered 10% formalin. We are in the process of switching to 10% neutral buffered formalin. There is some concern about salt build-up within the processor, as the cleaning cycle only consists of cleaning xylene and cleaning alcohol. I spoke to a representative at Leica who maintains that if you use 1 or 2 stations containing 70% alcohol immediately after the formain stations, that this is enough to prevent salt build-up from the buffered formalin. One of my technicians has some concern about this procedure. I would appreciate any feedback on this issue, especially from another lab using the Leica Peloris with 10% neutral buffered formalin. Thank you. ? Stacey Merica H.T. Histology Supervisor North Kansas City Hospital North Kansas City, MO From wbenton <@t> cua.md Mon Jul 15 12:19:28 2013 From: wbenton <@t> cua.md (Walter Benton) Date: Mon Jul 15 12:20:58 2013 Subject: [Histonet] (no subject) In-Reply-To: <1373908187.34444.YahooMailNeo@web184403.mail.bf1.yahoo.com> References: <1373908187.34444.YahooMailNeo@web184403.mail.bf1.yahoo.com> Message-ID: <0B8979A204680A42B93A52B486088CD938E883CBAE@CUAEXH1.GCU-MD.local> Stacey, In addition to the 70% step, many processors (not sure about this one had ways to perform a warm water flush) to rinse the lines of salt precipitate. Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) ChesapeakeUrology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of STACEY [staceyjm@sbcglobal.net] Sent: Monday, July 15, 2013 1:09 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Our laboratory has a Leica Peloris processor. Currently, we are using unbuffered 10% formalin. We are in the process of switching to 10% neutral buffered formalin. There is some concern about salt build-up within the processor, as the cleaning cycle only consists of cleaning xylene and cleaning alcohol. I spoke to a representative at Leica who maintains that if you use 1 or 2 stations containing 70% alcohol immediately after the formain stations, that this is enough to prevent salt build-up from the buffered formalin. One of my technicians has some concern about this procedure. I would appreciate any feedback on this issue, especially from another lab using the Leica Peloris with 10% neutral buffered formalin. Thank you. Stacey Merica H.T. Histology Supervisor North Kansas City Hospital North Kansas City, MO _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From tony.auge <@t> gmail.com Mon Jul 15 12:47:02 2013 From: tony.auge <@t> gmail.com (Tony Auge) Date: Mon Jul 15 12:47:08 2013 Subject: [Histonet] (no subject) In-Reply-To: <1373908187.34444.YahooMailNeo@web184403.mail.bf1.yahoo.com> References: <1373908187.34444.YahooMailNeo@web184403.mail.bf1.yahoo.com> Message-ID: I recently was having salt percipitation problems with my VIP proccesor and we were doing a hot water flush every week. We were starting with 90% alcohol and heat on the first station and also using 10% NBF. We switched to 50% in the first station and 70% in the second station. This has not only got rid of the salt problem but our small tissue biopsies look much better. I'm not sure what your technicians concerns are about but from my experience lowering the concentration of the first alcohols is more gentle for the smaller tissues but it might not processes big fatty specimens as well. There will be also more reagent carryover from the water introduced in the first station but is worth it in my opinion. Good Luck! Tony Auge HTL QIHC (ASCP) Cell: (651) 373-4768 Email: tony.auge@gmail.com From robinsoc <@t> mercyhealth.com Mon Jul 15 13:09:20 2013 From: robinsoc <@t> mercyhealth.com (Cynthia Robinson) Date: Mon Jul 15 13:09:34 2013 Subject: [Histonet] (no subject) In-Reply-To: References: <1373908187.34444.YahooMailNeo@web184403.mail.bf1.yahoo.com> Message-ID: <51E3F480020000AF0000EF7F@nodcdmg2.no.trinity-health.org> If you use anything higher than 70% alcohol after the 10% buffered formalin you will have salt precipitate out and cause all kinds of problems. We use 70%, 80%, 90% alcohol in the stations after the formalin and have not seen any issues with our Peloris or the VIPs. Cindi Robinson HT(ASCP) Mercy Medical Center Dunes Medical Laboratories 350 W Anchor Dr Dakota Dunes SD 57049 phone-712-279-2768 robinsoc@mercyhealth.com >>> Tony Auge 07/15/2013 12:47 >>> I recently was having salt percipitation problems with my VIP proccesor and we were doing a hot water flush every week. We were starting with 90% alcohol and heat on the first station and also using 10% NBF. We switched to 50% in the first station and 70% in the second station. This has not only got rid of the salt problem but our small tissue biopsies look much better. I'm not sure what your technicians concerns are about but from my experience lowering the concentration of the first alcohols is more gentle for the smaller tissues but it might not processes big fatty specimens as well. There will be also more reagent carryover from the water introduced in the first station but is worth it in my opinion. Good Luck! Tony Auge HTL QIHC (ASCP) Cell: (651) 373-4768 Email: tony.auge@gmail.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From annamhuntley <@t> gmail.com Mon Jul 15 16:13:02 2013 From: annamhuntley <@t> gmail.com (Anna Huntley Coffey) Date: Mon Jul 15 16:13:06 2013 Subject: [Histonet] Histonet mailing list submission: Jones' Methenamine Silver Stain Message-ID: Dear Histonetters, I was wondering if anyone has experience with Jones' Methenamine Silver stain? We have attempted this stain several times, each time with a slight tweak to the protocols we found online. We found that it took much longer than expected (3-4 hours compared to the expected 1 hour) for the membranes to develop the expected black color every attempt we made. The few times we did see the black color develop in the membranes, the next step in the gold chloride solution (1 minute) washed away all the dark color in the kidney, leaving it a very light brown. However, we did notice that only the skin sample on our test slides retained the black color. Since we ultimately need this stain for kidney samples, this didn't do us any good. We tried thinner sections (2 micron), acid washed glassware, brand new reagents, and multiple protocols with no luck. I would greatly appreciate any advice as to what we could try differently or if anyone else has experienced these same problems. Thank you! Anna From renafail2 <@t> gmail.com Mon Jul 15 18:06:53 2013 From: renafail2 <@t> gmail.com (Rena Fail) Date: Mon Jul 15 18:06:58 2013 Subject: [Histonet] Histonet mailing list submission: Jones' Methenamine Silver Stain In-Reply-To: References: Message-ID: Anna, place your coplin jar with silver solution in a 65 degree waterbath 5-10 minutes before your slides need to go in the sol. PreHeating the sol.in the waterbath and staining the slides in the waterbath will aid in reducing the time down to 10-15 minutes. make the methenamine- silver fresh. the silver and borax can be made ahead the methenamine should be made fresh each time., Don;t check the slides until the tissue looks golden brown.your end point is when the elsatic tissue is black (wlastic stains1st with the silver), Bowman's capsule is black and the basement membrane of the glomerlus is golden brown to black. the temptation is to stop when the bm is defined. Make sure the bowmans capsule is black and the bm in the glom. is dark golden brown to black. BM in thhte skin stains rapidly much faster than thhat in thhe glom Rena fail On Mon, Jul 15, 2013 at 5:13 PM, Anna Huntley Coffey wrote: > Dear Histonetters, > > I was wondering if anyone has experience with Jones' Methenamine Silver > stain? We have attempted this stain several times, each time with a slight > tweak to the protocols we found online. We found that it took much longer > than expected (3-4 hours compared to the expected 1 hour) for the membranes > to develop the expected black color every attempt we made. The few times > we did see the black color develop in the membranes, the next step in the > gold chloride solution (1 minute) washed away all the dark color in the > kidney, leaving it a very light brown. However, we did notice that only > the skin sample on our test slides retained the black color. Since we > ultimately need this stain for kidney samples, this didn't do us any good. > We tried thinner sections (2 micron), acid washed glassware, brand new > reagents, and multiple protocols with no luck. I would greatly appreciate > any advice as to what we could try differently or if anyone else has > experienced these same problems. > > Thank you! > Anna > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From tony.henwood <@t> health.nsw.gov.au Mon Jul 15 18:27:09 2013 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Mon Jul 15 18:27:36 2013 Subject: [Histonet] Histonet mailing list submission: Jones' Methenamine Silver Stain In-Reply-To: References: Message-ID: <6D6BD1DE8A5571489398B392A38A71579D28C113@xmdb04.nch.kids> Anna, I would try Hayashi et al (Stain Technol 64(4):185-190, 1989) technique. They have modified this procedure by including a treatment with thiosemicarbazide before silver impregnation. This enhances the deposition of silver, decreasing background staining. It is believed that the hydrazine group (-NHNH2) of thiosemicarbazide blocks the aldehyde group produced following periodic aid treatment and the thiocarbamyl group (-SCNH2) of thiosemicarbazide reduces the methenamine silver. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anna Huntley Coffey Sent: Tuesday, 16 July 2013 7:13 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histonet mailing list submission: Jones' Methenamine Silver Stain Dear Histonetters, I was wondering if anyone has experience with Jones' Methenamine Silver stain? We have attempted this stain several times, each time with a slight tweak to the protocols we found online. We found that it took much longer than expected (3-4 hours compared to the expected 1 hour) for the membranes to develop the expected black color every attempt we made. The few times we did see the black color develop in the membranes, the next step in the gold chloride solution (1 minute) washed away all the dark color in the kidney, leaving it a very light brown. However, we did notice that only the skin sample on our test slides retained the black color. Since we ultimately need this stain for kidney samples, this didn't do us any good. We tried thinner sections (2 micron), acid washed glassware, brand new reagents, and multiple protocols with no luck. I would greatly appreciate any advice as to what we could try differently or if anyone else has experienced these same problems. Thank you! Anna _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From tony.henwood <@t> health.nsw.gov.au Mon Jul 15 18:33:35 2013 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Mon Jul 15 18:33:52 2013 Subject: [Histonet] RE: non-xylene dewaxing. In-Reply-To: <7722595275A4DD4FA225B92CDBF174A101B0D08CC282@EXC-MBX3.cfs.le.ac.uk> References: <7722595275A4DD4FA225B92CDBF174A101B0D08CC282@EXC-MBX3.cfs.le.ac.uk> Message-ID: <6D6BD1DE8A5571489398B392A38A71579D28C15C@xmdb04.nch.kids> Hi Richard, I have attached two papers that might be useful Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Edwards, Richard E. Sent: Monday, 15 July 2013 7:55 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] non-xylene dewaxing. Hi All, any preferred methods?, all information gratefully received, many thanks. Richard Edwards University of Leicester U.K. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From histo <@t> pathlab.us Tue Jul 16 06:14:08 2013 From: histo <@t> pathlab.us (Histology) Date: Tue Jul 16 06:14:13 2013 Subject: [Histonet] Competency for Anatomic and Clinical Pathology at Message-ID: <9C0B151B30AC5F4ABFD1B69D915F84CF33E6D4@dc01.DominionPath.local> Hi, I would love to see your competency spreadsheet for histology. We just finished our first CAP inspection and got a deficiency here. He said the direct observation was great, but that we need to have all 6 elements. I am having trouble trying to come up with a way to evaluate some of these and would love any help. Thanks in advance, Mehndi Helgren Dominion Pathology Laboratories 733 Boush St. Suite 200 Norfolk, VA 23510 757-664-7901 histo@pathlab.us From gu.lang <@t> gmx.at Tue Jul 16 10:43:17 2013 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Tue Jul 16 10:57:03 2013 Subject: AW: [Histonet] Histonet mailing list submission: Jones' Methenamine Silver Stain In-Reply-To: References: Message-ID: <000001ce823b$30efb420$92cf1c60$@gmx.at> Hi Anna! We had good success with following protocol: Deparaffination, rehydration to dest. Water 0,5 % Periodic acid 20 min Prewarm Methenamin-Silver-Solution without Borax in 60?C oven Rinse slides in dest. Water 3x 5 min Add Borax to Methenamin-Silver Solution Put slides into Methenamin-Silver-Working solution for 45 min Prewarm coplin jar with dest. Water in 60?C oven Rinse slides in prewarmed water Let cool for 10 min 0,1 % goldchloride 10 min Rinse in dest. Water 2% Sodiumthiosulfate 5 sek Rinse in running tapwater 5 min Counterstain with hematoxylin if wanted. Etc, Methenamin-silver-Stock: 400 ml 3% hexamethylentetramin (CAS 100-97-0) 10 ml 10% silvernitrate (CAS 7761-88-8) Solution turns first cloudy, then clear. Usefull for half a year. Methenamin-silver-Workingsolution: 80 ml Silver-Stock 8 ml Borax (=sodiumtetraborate CAS 1303-96-4) Gives a pH of 9 During incubation in silversolution a "mirror" developes onto the slides and the coplin jars. Just when this point is reached, the reaction is enough and ready. This turning point occurs suddenly. You have to watch the jar every few minutes after the first 40-45 min. Silversolution without Borax and pH 9 bring no silver-reaction. Hope this helps Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Anna Huntley Coffey Gesendet: Montag, 15. Juli 2013 23:13 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Histonet mailing list submission: Jones' Methenamine Silver Stain Dear Histonetters, I was wondering if anyone has experience with Jones' Methenamine Silver stain? We have attempted this stain several times, each time with a slight tweak to the protocols we found online. We found that it took much longer than expected (3-4 hours compared to the expected 1 hour) for the membranes to develop the expected black color every attempt we made. The few times we did see the black color develop in the membranes, the next step in the gold chloride solution (1 minute) washed away all the dark color in the kidney, leaving it a very light brown. However, we did notice that only the skin sample on our test slides retained the black color. Since we ultimately need this stain for kidney samples, this didn't do us any good. We tried thinner sections (2 micron), acid washed glassware, brand new reagents, and multiple protocols with no luck. I would greatly appreciate any advice as to what we could try differently or if anyone else has experienced these same problems. Thank you! Anna _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From KSimeone <@t> leavittmgt.com Tue Jul 16 11:23:48 2013 From: KSimeone <@t> leavittmgt.com (Delray Beach Pathology Kari Simeone) Date: Tue Jul 16 11:24:09 2013 Subject: [Histonet] HHV8 control slides Message-ID: Hi Histonetters! Can anyone recommend a place where I can purchase HHV8 control slides for IHC? I usually purchase from Cell Marque but they are back ordered. Thanks! :-) Kari M Simeone Histology/Immunohistochemistry Specialist Supervisor Alternate Laboratory Supervisor ADCS Clinics, LLC Advanced Dermatology and Cosmetic Surgery Ameriderm 561.819.0857 ext 100 561.819.6517 fax ksimeone@leavittmgt.com www.advancedderm.com The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message. From CObregon <@t> mhs.net Tue Jul 16 11:38:03 2013 From: CObregon <@t> mhs.net (Obregon, Cecilia) Date: Tue Jul 16 11:38:24 2013 Subject: [Histonet] RE: HHV8 control slides In-Reply-To: References: Message-ID: <598BB4A3A92D7F4DAA14C30E4AE70C2F10B1D0B6@MHSEXMB05.mhs.net> We purchase control slides for HSV, PCP, Gram, AFB from Mercedes Medical. Here's the contact info: Mercedes Medical Ph: 800-331-2716 www.mercedesmedical.com Cecilia M. Obregon, HTL(ASCP) QIHC Memorial Regional Hospital 3501 Johnson Street Hollywood, FL 33021 Ph: 954-265-5317 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Delray Beach Pathology Kari Simeone [KSimeone@leavittmgt.com] Sent: Tuesday, July 16, 2013 12:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HHV8 control slides Hi Histonetters! Can anyone recommend a place where I can purchase HHV8 control slides for IHC? I usually purchase from Cell Marque but they are back ordered. Thanks! :-) Kari M Simeone Histology/Immunohistochemistry Specialist Supervisor Alternate Laboratory Supervisor ADCS Clinics, LLC Advanced Dermatology and Cosmetic Surgery Ameriderm 561.819.0857 ext 100 561.819.6517 fax ksimeone@leavittmgt.com www.advancedderm.com The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From foreightl <@t> gmail.com Tue Jul 16 11:39:36 2013 From: foreightl <@t> gmail.com (Patrick Laurie) Date: Tue Jul 16 11:39:39 2013 Subject: [Histonet] Competency for Anatomic and Clinical Pathology at In-Reply-To: <9C0B151B30AC5F4ABFD1B69D915F84CF33E6D4@dc01.DominionPath.local> References: <9C0B151B30AC5F4ABFD1B69D915F84CF33E6D4@dc01.DominionPath.local> Message-ID: Hi Mehndi, The Michigan Society of Histotechnologists publishes a competency assessment handbook that helped us set our competency procedure up. It is a relatively small fee, but it is very detailed, has histology and gross room suggestions. Patrick On Tue, Jul 16, 2013 at 7:14 AM, Histology wrote: > Hi, > > > > I would love to see your competency spreadsheet for histology. We just > finished our first CAP inspection and got a deficiency here. He said > the direct observation was great, but that we need to have all 6 > elements. I am having trouble trying to come up with a way to evaluate > some of these and would love any help. > > > > Thanks in advance, > > > > Mehndi Helgren > > Dominion Pathology Laboratories > > 733 Boush St. > > Suite 200 > > Norfolk, VA 23510 > > 757-664-7901 > > histo@pathlab.us > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie(HT)ASCP QIHC Histology Manager Celligent Diagnostics, LLC 101 East W.T. Harris Blvd | Suite 1212 | Charlotte, NC 28262 Work: 704-970-3300 Cell: 704-266-0869 From amber.mckenzie <@t> gastrodocs.net Tue Jul 16 14:24:50 2013 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Tue Jul 16 14:24:53 2013 Subject: [Histonet] Sub-optimal Results In-Reply-To: <1371396807.72763.YahooMailNeo@web163102.mail.bf1.yahoo.com> References: <1371396807.72763.YahooMailNeo@web163102.mail.bf1.yahoo.com> Message-ID: <5A33C952BB67F4468AF1F36D739212BCBD6BB13C@JERRY.Gia.com> Do you guys keep the QA form when a specimen is rejected and sent back to the source for correction? ANP. 11475 From patrick.lewis <@t> seattlechildrens.org Tue Jul 16 15:02:45 2013 From: patrick.lewis <@t> seattlechildrens.org (Lewis, Patrick) Date: Tue Jul 16 15:02:53 2013 Subject: [Histonet] Searching for HHV-7 antibody Message-ID: <3903BE18914F4440834F0E620415FFCA25AC7AF1@PPWEXD01a.childrens.sea.kids> HI everyone, Does anyone know where to get 5E1 mouse monoclonal antibody directed against HHV-7 (pp85 protein complex) We tried two suppliers but their product has been discontinued and now we can not seem to find it anywhere. Thanks Patrick. CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From JMacDonald <@t> mtsac.edu Tue Jul 16 15:47:16 2013 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Tue Jul 16 15:47:24 2013 Subject: [Histonet] Positions in SF area Message-ID: I have a graduate that is looking for a Histotechnician position in the Bay area. He is currently working for a dermatology lab. He would like to relocate to the Bay area. If you know of any positions would you please let me know. Thank you, Jennifer MacDonald Mt. San Antonio College 909-274-4884 jmacdonald@mtsac.edu From LSebree <@t> uwhealth.org Tue Jul 16 16:51:08 2013 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Tue Jul 16 16:51:12 2013 Subject: [Histonet] RE: Sub-optimal Results In-Reply-To: <5A33C952BB67F4468AF1F36D739212BCBD6BB13C@JERRY.Gia.com> References: <1371396807.72763.YahooMailNeo@web163102.mail.bf1.yahoo.com> <5A33C952BB67F4468AF1F36D739212BCBD6BB13C@JERRY.Gia.com> Message-ID: <77DD817201982748BC67D7960F2F76AF051CC3@UWHC-MBX12.uwhis.hosp.wisc.edu> We are required to keep all QA forms for 2 years. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Tuesday, July 16, 2013 2:25 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sub-optimal Results Do you guys keep the QA form when a specimen is rejected and sent back to the source for correction? ANP. 11475 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Tue Jul 16 17:23:35 2013 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Tue Jul 16 17:23:40 2013 Subject: [Histonet] Sub-optimal Results In-Reply-To: <5A33C952BB67F4468AF1F36D739212BCBD6BB13C@JERRY.Gia.com> References: , <1371396807.72763.YahooMailNeo@web163102.mail.bf1.yahoo.com>, <5A33C952BB67F4468AF1F36D739212BCBD6BB13C@JERRY.Gia.com> Message-ID: Yes absolutely. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: amber.mckenzie@gastrodocs.net > To: histonet@lists.utsouthwestern.edu > Date: Tue, 16 Jul 2013 19:24:50 +0000 > Subject: [Histonet] Sub-optimal Results > > Do you guys keep the QA form when a specimen is rejected and sent back to the source for correction? ANP. 11475 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MICHELLE.LAMPHERE <@t> childrens.com Wed Jul 17 08:10:02 2013 From: MICHELLE.LAMPHERE <@t> childrens.com (Michelle Lamphere) Date: Wed Jul 17 08:10:06 2013 Subject: [Histonet] RE: Competency for Anatomic and Clinical Pathology Message-ID: I think a few people might find this interesting.... I recently attended a class about Competency Assessments in the lab. The class was given by Ken Byrd (fairly certain that is how you spell his name), a Senior Inspector at CAP. When this particular question came up, I asked him to give examples of how the histology lab was supposed to use the 6 elements to assess competency. He informed the entire class that the competency assessment question with the six elements did not apply to the histology lab because histology did not report test results. It is the one question on the Gen Lab checklist that did not apply to ANP. Kinda shocking, I know. It does not mean that we scrapped our entire competency program, we simply removed some of the six elements. Michelle Lamphere Senior Tech, Histology Anatomic Pathology Children's Medical Center O: 214.456.2318 | Fax: 214.456.0779 E: michelle.lamphere@childrens.com 1935 Medical District Drive | B1.06 ?| Dallas, Texas 75235 Message: 9 Date: Tue, 16 Jul 2013 07:14:08 -0400 From: "Histology" Subject: [Histonet] Competency for Anatomic and Clinical Pathology at To: Message-ID: <9C0B151B30AC5F4ABFD1B69D915F84CF33E6D4@dc01.DominionPath.local> Content-Type: text/plain; charset="us-ascii" Hi, I would love to see your competency spreadsheet for histology. We just finished our first CAP inspection and got a deficiency here. He said the direct observation was great, but that we need to have all 6 elements. I am having trouble trying to come up with a way to evaluate some of these and would love any help. Thanks in advance, Mehndi Helgren Dominion Pathology Laboratories 733 Boush St. Suite 200 Norfolk, VA 23510 757-664-7901 histo@pathlab.us Please consider the environment before printing this e-mail. This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. From mbmphoto <@t> gmail.com Wed Jul 17 09:12:11 2013 From: mbmphoto <@t> gmail.com (Maria Mejia) Date: Wed Jul 17 09:12:20 2013 Subject: [Histonet] microtome aligner In-Reply-To: References: , <1371396807.72763.YahooMailNeo@web163102.mail.bf1.yahoo.com>, <5A33C952BB67F4468AF1F36D739212BCBD6BB13C@JERRY.Gia.com> Message-ID: <7814CEF8-9D00-4B49-BCB6-FEDD10559178@gmail.com> Hello, Can anyone provide any additional information regarding microtome aligners? Do they work? I have never used or seen this particular tool, so which brand works best? I know of only one company that sells a microtome aligner (universal type). Any information any one can provide will be most appreciated. Best Maria Mejia San Francisco, CA From Timothy.Morken <@t> ucsfmedctr.org Wed Jul 17 10:09:10 2013 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Wed Jul 17 10:10:47 2013 Subject: [Histonet] RE: Competency for Anatomic and Clinical Pathology In-Reply-To: References: Message-ID: <761E2B5697F795489C8710BCC72141FF08704F@ex07.net.ucsf.edu> Yes, that is "interesting" considering that competencies are some of the primary documents that the Joint Commission inspectors ask for every time. And JC is under the same rules as CAP. So what is CAP's deal? BTW, What was this class focused on? Not histology? Clincal lab, cytology? Curious. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michelle Lamphere Sent: Wednesday, July 17, 2013 6:10 AM To: 'histonet@lists.utsouthwestern.edu' Cc: Elma Cortinas Subject: [Histonet] RE: Competency for Anatomic and Clinical Pathology I think a few people might find this interesting.... I recently attended a class about Competency Assessments in the lab. The class was given by Ken Byrd (fairly certain that is how you spell his name), a Senior Inspector at CAP. When this particular question came up, I asked him to give examples of how the histology lab was supposed to use the 6 elements to assess competency. He informed the entire class that the competency assessment question with the six elements did not apply to the histology lab because histology did not report test results. It is the one question on the Gen Lab checklist that did not apply to ANP. Kinda shocking, I know. It does not mean that we scrapped our entire competency program, we simply removed some of the six elements. Michelle Lamphere Senior Tech, Histology Anatomic Pathology Children's Medical Center O: 214.456.2318 | Fax: 214.456.0779 E: michelle.lamphere@childrens.com 1935 Medical District Drive | B1.06 ?| Dallas, Texas 75235 Message: 9 Date: Tue, 16 Jul 2013 07:14:08 -0400 From: "Histology" Subject: [Histonet] Competency for Anatomic and Clinical Pathology at To: Message-ID: <9C0B151B30AC5F4ABFD1B69D915F84CF33E6D4@dc01.DominionPath.local> Content-Type: text/plain; charset="us-ascii" Hi, I would love to see your competency spreadsheet for histology. We just finished our first CAP inspection and got a deficiency here. He said the direct observation was great, but that we need to have all 6 elements. I am having trouble trying to come up with a way to evaluate some of these and would love any help. Thanks in advance, Mehndi Helgren Dominion Pathology Laboratories 733 Boush St. Suite 200 Norfolk, VA 23510 757-664-7901 histo@pathlab.us Please consider the environment before printing this e-mail. This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From algranth <@t> email.arizona.edu Wed Jul 17 10:14:16 2013 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Wed Jul 17 10:14:21 2013 Subject: [Histonet] microtome aligner In-Reply-To: <7814CEF8-9D00-4B49-BCB6-FEDD10559178@gmail.com> References: , <1371396807.72763.YahooMailNeo@web163102.mail.bf1.yahoo.com>, <5A33C952BB67F4468AF1F36D739212BCBD6BB13C@JERRY.Gia.com> <7814CEF8-9D00-4B49-BCB6-FEDD10559178@gmail.com> Message-ID: <84CFE38D-CA15-4427-A370-D442ECE8CFE4@email.arizona.edu> They do work. I saw one at NSH some years ago and came home and had the guys in our shop make one for me exactly like the one I saw and it cost next to nothing. We bought the little round level at home depot. Andi Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 From TGoins <@t> mt.gov Wed Jul 17 10:59:52 2013 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Wed Jul 17 11:00:57 2013 Subject: [Histonet] microtome aligner In-Reply-To: <7814CEF8-9D00-4B49-BCB6-FEDD10559178@gmail.com> References: , <1371396807.72763.YahooMailNeo@web163102.mail.bf1.yahoo.com>, <5A33C952BB67F4468AF1F36D739212BCBD6BB13C@JERRY.Gia.com> <7814CEF8-9D00-4B49-BCB6-FEDD10559178@gmail.com> Message-ID: Yikes. There is a lot of "play" getting that bubble in the middle of the "universal" aligner that may translate to large variations in alignment each time the thing is used. The high cost is probably for the clamp, but if the bubble level is worth $1.50 the quality of the clamp doesn't matter. Save your money and use a blank paraffin block. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Maria Mejia Sent: Wednesday, July 17, 2013 8:12 AM To: HistoNet Subject: [Histonet] microtome aligner Hello, Can anyone provide any additional information regarding microtome aligners? Do they work? I have never used or seen this particular tool, so which brand works best? I know of only one company that sells a microtome aligner (universal type). Any information any one can provide will be most appreciated. Best Maria Mejia San Francisco, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From patpxs <@t> gmail.com Wed Jul 17 11:13:07 2013 From: patpxs <@t> gmail.com (Paula Sicurello) Date: Wed Jul 17 11:13:11 2013 Subject: [Histonet] microtome aligner In-Reply-To: <84CFE38D-CA15-4427-A370-D442ECE8CFE4@email.arizona.edu> References: <1371396807.72763.YahooMailNeo@web163102.mail.bf1.yahoo.com> <5A33C952BB67F4468AF1F36D739212BCBD6BB13C@JERRY.Gia.com> <7814CEF8-9D00-4B49-BCB6-FEDD10559178@gmail.com> <84CFE38D-CA15-4427-A370-D442ECE8CFE4@email.arizona.edu> Message-ID: I used an even cheaper version of the aligner. My microtome service engineer suggested an "L level" or a "T" level that you can buy at Home Depot. The L looks just like an L, it is actually a right angle measuring device, used to make sure the corners or something are straight at 90 degrees. You take the knife holder out and place one side of the L on the knife holder place and the other against the block face. Paula :-) -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 HIPAA Privacy Notification: This message and any accompanying documents are covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, and contain information intended for the specific individual (s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying or the taking of any action based on the contents of this information is strictly prohibited . If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. On Wed, Jul 17, 2013 at 11:14 AM, Grantham, Andrea L - (algranth) < algranth@email.arizona.edu> wrote: > They do work. I saw one at NSH some years ago and came home and had the > guys in our shop make one for me exactly like the one I saw and it cost > next to nothing. We bought the little round level at home depot. > > Andi > > > > > Andrea Grantham, HT (ASCP) > Senior Research Specialist > University of Arizona > Cellular and Molecular Medicine > Histology Service Laboratory > P.O.Box 245044 > Tucson, AZ 85724 > > algranth@email.arizona.edu > Tel: 520.626.4415 Fax: 520.626.2097 > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From joelleweaver <@t> hotmail.com Wed Jul 17 11:34:05 2013 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Wed Jul 17 11:34:12 2013 Subject: [Histonet] microtome aligner In-Reply-To: <84CFE38D-CA15-4427-A370-D442ECE8CFE4@email.arizona.edu> References: , , <1371396807.72763.YahooMailNeo@web163102.mail.bf1.yahoo.com>, , <5A33C952BB67F4468AF1F36D739212BCBD6BB13C@JERRY.Gia.com>, , <7814CEF8-9D00-4B49-BCB6-FEDD10559178@gmail.com>, <84CFE38D-CA15-4427-A370-D442ECE8CFE4@email.arizona.edu> Message-ID: Yes you can certainly align the old fashioned way easy enough, but I find these are very handy and do save some time. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: algranth@email.arizona.edu > CC: histonet@lists.utsouthwestern.edu > Date: Wed, 17 Jul 2013 15:14:16 +0000 > Subject: Re: [Histonet] microtome aligner > > They do work. I saw one at NSH some years ago and came home and had the guys in our shop make one for me exactly like the one I saw and it cost next to nothing. We bought the little round level at home depot. > > Andi > > > > > Andrea Grantham, HT (ASCP) > Senior Research Specialist > University of Arizona > Cellular and Molecular Medicine > Histology Service Laboratory > P.O.Box 245044 > Tucson, AZ 85724 > > algranth@email.arizona.edu > Tel: 520.626.4415 Fax: 520.626.2097 > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vavalos <@t> allergydermatology.com Wed Jul 17 11:35:29 2013 From: vavalos <@t> allergydermatology.com (Vanessa Avalos) Date: Wed Jul 17 11:35:49 2013 Subject: [Histonet] Lab Equipment Repair in AZ?? Message-ID: <6a98ea2a7359418fa1bcbb0d57a879b7@BY2PR07MB217.namprd07.prod.outlook.com> We recently found out that our equipment repair tech has retired. For our PM's and small repairs of our non contracted lab equipment we have used a local company for years. Sad to see him go. Does any one have any other local medical lab equipment techs/companies out in AZ (Phoenix) they would recommend? I am not looking for reps to offer warranties, only recommendations at this time. Thanks in advance! V.Avalos ADS, INC Fax:602-277-2134 From leila.etemadi <@t> med.lu.se Wed Jul 17 11:39:31 2013 From: leila.etemadi <@t> med.lu.se (Leila Etemadi) Date: Wed Jul 17 11:39:37 2013 Subject: [Histonet] My perfusion problem Message-ID: <52551199-3F4A-451B-A02E-0D6B06529AA6@med.lu.se> Hi, I am desperately looking for an answer to my problem in perfusion procedure. What is my problem? To explain that I thought first I may clear what is my usual routin. I used to perfuse rat's brain through this procedure: 1- Anesthetise animal with sufficient amount of pentobarbital. 2- Clamp the vessel lying beside vertebra in the back, and then using a perfusion pump with an 17 or 18 gauge blunted needle inserted into the ascending aorta. 3- Washout blood with 0.9% salin ( room temperature), 150-200 ml, 76 rpm speed ( usually I wait to see the out put solution from the heart is not looking so red/ blood look like). 4- Switch to the 4% cold paraformaldehyde ( with buffer, 7.4 pH) ( on ice cold), 150-200 ml, 46 rpm speed. ( some times I can see the formalin dance but not always !). 5- after using this amount of PFA normally the neck part is quite stiff, then animal will be decapitated. I extract the brain right away, postfix in PFA over the night ( 4?c in the refrigerator). 6- Move to the sucrose 20%. When I saw brain is sinking in the solution ( which normally it happens in one and half day), I freeze the brain on dry ice quickly. The results of this procedure is good enough histology after dissection with cryostat ( 12-20?m). For my recent project I need brain and spinal cord both so basically I skip clamping the back along vertebra to circulate solution through whole body ( I didn't change any other steps, so I do exactly whatever I mentioned above except for spinal cord post fixation in PFA is about 2 hours). After sectioning ( 16?m), I've got a proper tissue from spinal cord but when it comes to the brain result is quite different !!, when I replaced my sections on the slide ( with plus charge), suddenly a lot of hole began to shaped in the tissue which practically ruined the entire piece..!!!, First I had no clue for what I could see but then I noticed during post fixation procedure, when I transfer brain from PFA solution to the sucrose, brain is sinking right away ( it is not floating at all, as it suppose to do..) !!!!! So I've run another perfusion procedure but this time when I reached to the washing out by cold PFA I didn't decreased the pump speed ( as normally I decrease it from 76 rpm to 46 rpm, this time I run whole procedure with the speed of 76 rpm), on the other hand I used more PFA solution ( about 350 ml PFA). After extracting brain I noticed brain was looking dried up. during post fixation and cryoprotection procedure (sucrose solution), sinking was looking normal, but after sectioning of course brain tissue was destroyed again!!! Now, I need your expert advices for my problem. I apologise for my naivety on this subject in advance. Humbly appreciate for your great time and attention in advance. I severely looking forward to your help and valuable tips. With the best, Leila Leila Etemadi M.Sc., Ph.D Candidate Neuronano Research Center (NRC) Lund University, BMC F10 Sweden Tel: +46-46-2221503 E-mail: Leila.Etemadi@med.lu.se Sent from my iPad From donna_suresch <@t> merck.com Wed Jul 17 12:34:22 2013 From: donna_suresch <@t> merck.com (Suresch, Donna L.) Date: Wed Jul 17 12:34:35 2013 Subject: [Histonet] RE: Histonet Digest, Vol 116, Issue 16 In-Reply-To: References: Message-ID: <2C9A1D9608959940943F357E0A470FF8BD8FC7060F@USCTMXP51005.merck.com> RE: Microtome Aligners Maria, Those microtome aligners will only be helpful in aligning all your microtomes to the same angle. They do not help you find the optimal angle. Donna L. Suresch Senior Imaging Research Scientist Merck Research Laboratories Department of Imaging - West Point Campus -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Wednesday, July 17, 2013 1:02 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 116, Issue 16 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Sub-optimal Results (Amber McKenzie) 2. Searching for HHV-7 antibody (Lewis, Patrick) 3. Positions in SF area (Jennifer MacDonald) 4. RE: Sub-optimal Results (Sebree Linda A) 5. RE: Sub-optimal Results (joelle weaver) 6. RE: Competency for Anatomic and Clinical Pathology (Michelle Lamphere) 7. microtome aligner (Maria Mejia) 8. RE: Competency for Anatomic and Clinical Pathology (Morken, Timothy) 9. Re: microtome aligner (Grantham, Andrea L - (algranth)) 10. RE: microtome aligner (Goins, Tresa) 11. Re: microtome aligner (Paula Sicurello) 12. RE: microtome aligner (joelle weaver) 13. Lab Equipment Repair in AZ?? (Vanessa Avalos) 14. My perfusion problem (Leila Etemadi) ---------------------------------------------------------------------- Message: 1 Date: Tue, 16 Jul 2013 19:24:50 +0000 From: Amber McKenzie Subject: [Histonet] Sub-optimal Results To: "histonet@lists.utsouthwestern.edu" Message-ID: <5A33C952BB67F4468AF1F36D739212BCBD6BB13C@JERRY.Gia.com> Content-Type: text/plain; charset="us-ascii" Do you guys keep the QA form when a specimen is rejected and sent back to the source for correction? ANP. 11475 ------------------------------ Message: 2 Date: Tue, 16 Jul 2013 20:02:45 +0000 From: "Lewis, Patrick" Subject: [Histonet] Searching for HHV-7 antibody To: "'Histonet@lists.utsouthwestern.edu'" Message-ID: <3903BE18914F4440834F0E620415FFCA25AC7AF1@PPWEXD01a.childrens.sea.kids> Content-Type: text/plain; charset="us-ascii" HI everyone, Does anyone know where to get 5E1 mouse monoclonal antibody directed against HHV-7 (pp85 protein complex) We tried two suppliers but their product has been discontinued and now we can not seem to find it anywhere. Thanks Patrick. CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ------------------------------ Message: 3 Date: Tue, 16 Jul 2013 13:47:16 -0700 From: Jennifer MacDonald Subject: [Histonet] Positions in SF area To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" I have a graduate that is looking for a Histotechnician position in the Bay area. He is currently working for a dermatology lab. He would like to relocate to the Bay area. If you know of any positions would you please let me know. Thank you, Jennifer MacDonald Mt. San Antonio College 909-274-4884 jmacdonald@mtsac.edu ------------------------------ Message: 4 Date: Tue, 16 Jul 2013 21:51:08 +0000 From: Sebree Linda A Subject: [Histonet] RE: Sub-optimal Results To: 'Amber McKenzie' , "histonet@lists.utsouthwestern.edu" Message-ID: <77DD817201982748BC67D7960F2F76AF051CC3@UWHC-MBX12.uwhis.hosp.wisc.edu> Content-Type: text/plain; charset="us-ascii" We are required to keep all QA forms for 2 years. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Tuesday, July 16, 2013 2:25 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sub-optimal Results Do you guys keep the QA form when a specimen is rejected and sent back to the source for correction? ANP. 11475 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Tue, 16 Jul 2013 22:23:35 +0000 From: joelle weaver Subject: RE: [Histonet] Sub-optimal Results To: Amber McKenzie , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Yes absolutely. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: amber.mckenzie@gastrodocs.net > To: histonet@lists.utsouthwestern.edu > Date: Tue, 16 Jul 2013 19:24:50 +0000 > Subject: [Histonet] Sub-optimal Results > > Do you guys keep the QA form when a specimen is rejected and sent back to the source for correction? ANP. 11475 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Wed, 17 Jul 2013 13:10:02 +0000 From: Michelle Lamphere Subject: [Histonet] RE: Competency for Anatomic and Clinical Pathology To: "'histonet@lists.utsouthwestern.edu'" Cc: Elma Cortinas Message-ID: Content-Type: text/plain; charset="iso-8859-1" I think a few people might find this interesting.... I recently attended a class about Competency Assessments in the lab. The class was given by Ken Byrd (fairly certain that is how you spell his name), a Senior Inspector at CAP. When this particular question came up, I asked him to give examples of how the histology lab was supposed to use the 6 elements to assess competency. He informed the entire class that the competency assessment question with the six elements did not apply to the histology lab because histology did not report test results. It is the one question on the Gen Lab checklist that did not apply to ANP. Kinda shocking, I know. It does not mean that we scrapped our entire competency program, we simply removed some of the six elements. Michelle Lamphere Senior Tech, Histology Anatomic Pathology Children's Medical Center O: 214.456.2318 | Fax: 214.456.0779 E: michelle.lamphere@childrens.com 1935 Medical District Drive | B1.06 ?| Dallas, Texas 75235 Message: 9 Date: Tue, 16 Jul 2013 07:14:08 -0400 From: "Histology" Subject: [Histonet] Competency for Anatomic and Clinical Pathology at To: Message-ID: <9C0B151B30AC5F4ABFD1B69D915F84CF33E6D4@dc01.DominionPath.local> Content-Type: text/plain; charset="us-ascii" Hi, I would love to see your competency spreadsheet for histology. We just finished our first CAP inspection and got a deficiency here. He said the direct observation was great, but that we need to have all 6 elements. I am having trouble trying to come up with a way to evaluate some of these and would love any help. Thanks in advance, Mehndi Helgren Dominion Pathology Laboratories 733 Boush St. Suite 200 Norfolk, VA 23510 757-664-7901 histo@pathlab.us Please consider the environment before printing this e-mail. This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. ------------------------------ Message: 7 Date: Wed, 17 Jul 2013 07:12:11 -0700 From: Maria Mejia Subject: [Histonet] microtome aligner To: HistoNet Message-ID: <7814CEF8-9D00-4B49-BCB6-FEDD10559178@gmail.com> Content-Type: text/plain; charset=iso-8859-1 Hello, Can anyone provide any additional information regarding microtome aligners? Do they work? I have never used or seen this particular tool, so which brand works best? I know of only one company that sells a microtome aligner (universal type). Any information any one can provide will be most appreciated. Best Maria Mejia San Francisco, CA ------------------------------ Message: 8 Date: Wed, 17 Jul 2013 15:09:10 +0000 From: "Morken, Timothy" Subject: [Histonet] RE: Competency for Anatomic and Clinical Pathology To: "Michelle Lamphere" , "'histonet@lists.utsouthwestern.edu'" Message-ID: <761E2B5697F795489C8710BCC72141FF08704F@ex07.net.ucsf.edu> Content-Type: text/plain; charset=iso-8859-1 Yes, that is "interesting" considering that competencies are some of the primary documents that the Joint Commission inspectors ask for every time. And JC is under the same rules as CAP. So what is CAP's deal? BTW, What was this class focused on? Not histology? Clincal lab, cytology? Curious. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michelle Lamphere Sent: Wednesday, July 17, 2013 6:10 AM To: 'histonet@lists.utsouthwestern.edu' Cc: Elma Cortinas Subject: [Histonet] RE: Competency for Anatomic and Clinical Pathology I think a few people might find this interesting.... I recently attended a class about Competency Assessments in the lab. The class was given by Ken Byrd (fairly certain that is how you spell his name), a Senior Inspector at CAP. When this particular question came up, I asked him to give examples of how the histology lab was supposed to use the 6 elements to assess competency. He informed the entire class that the competency assessment question with the six elements did not apply to the histology lab because histology did not report test results. It is the one question on the Gen Lab checklist that did not apply to ANP. Kinda shocking, I know. It does not mean that we scrapped our entire competency program, we simply removed some of the six elements. Michelle Lamphere Senior Tech, Histology Anatomic Pathology Children's Medical Center O: 214.456.2318 | Fax: 214.456.0779 E: michelle.lamphere@childrens.com 1935 Medical District Drive | B1.06 ?| Dallas, Texas 75235 Message: 9 Date: Tue, 16 Jul 2013 07:14:08 -0400 From: "Histology" Subject: [Histonet] Competency for Anatomic and Clinical Pathology at To: Message-ID: <9C0B151B30AC5F4ABFD1B69D915F84CF33E6D4@dc01.DominionPath.local> Content-Type: text/plain; charset="us-ascii" Hi, I would love to see your competency spreadsheet for histology. We just finished our first CAP inspection and got a deficiency here. He said the direct observation was great, but that we need to have all 6 elements. I am having trouble trying to come up with a way to evaluate some of these and would love any help. Thanks in advance, Mehndi Helgren Dominion Pathology Laboratories 733 Boush St. Suite 200 Norfolk, VA 23510 757-664-7901 histo@pathlab.us Please consider the environment before printing this e-mail. This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Wed, 17 Jul 2013 15:14:16 +0000 From: "Grantham, Andrea L - (algranth)" Subject: Re: [Histonet] microtome aligner Cc: HistoNet Message-ID: <84CFE38D-CA15-4427-A370-D442ECE8CFE4@email.arizona.edu> Content-Type: text/plain; charset="us-ascii" They do work. I saw one at NSH some years ago and came home and had the guys in our shop make one for me exactly like the one I saw and it cost next to nothing. We bought the little round level at home depot. Andi Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 ------------------------------ Message: 10 Date: Wed, 17 Jul 2013 15:59:52 +0000 From: "Goins, Tresa" Subject: RE: [Histonet] microtome aligner To: Maria Mejia , HistoNet Message-ID: Content-Type: text/plain; charset="us-ascii" Yikes. There is a lot of "play" getting that bubble in the middle of the "universal" aligner that may translate to large variations in alignment each time the thing is used. The high cost is probably for the clamp, but if the bubble level is worth $1.50 the quality of the clamp doesn't matter. Save your money and use a blank paraffin block. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Maria Mejia Sent: Wednesday, July 17, 2013 8:12 AM To: HistoNet Subject: [Histonet] microtome aligner Hello, Can anyone provide any additional information regarding microtome aligners? Do they work? I have never used or seen this particular tool, so which brand works best? I know of only one company that sells a microtome aligner (universal type). Any information any one can provide will be most appreciated. Best Maria Mejia San Francisco, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Wed, 17 Jul 2013 12:13:07 -0400 From: Paula Sicurello Subject: Re: [Histonet] microtome aligner To: "Grantham, Andrea L - (algranth)" Cc: HistoNet Message-ID: Content-Type: text/plain; charset=ISO-8859-1 I used an even cheaper version of the aligner. My microtome service engineer suggested an "L level" or a "T" level that you can buy at Home Depot. The L looks just like an L, it is actually a right angle measuring device, used to make sure the corners or something are straight at 90 degrees. You take the knife holder out and place one side of the L on the knife holder place and the other against the block face. Paula :-) -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 HIPAA Privacy Notification: This message and any accompanying documents are covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, and contain information intended for the specific individual (s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying or the taking of any action based on the contents of this information is strictly prohibited . If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. On Wed, Jul 17, 2013 at 11:14 AM, Grantham, Andrea L - (algranth) < algranth@email.arizona.edu> wrote: > They do work. I saw one at NSH some years ago and came home and had the > guys in our shop make one for me exactly like the one I saw and it cost > next to nothing. We bought the little round level at home depot. > > Andi > > > > > Andrea Grantham, HT (ASCP) > Senior Research Specialist > University of Arizona > Cellular and Molecular Medicine > Histology Service Laboratory > P.O.Box 245044 > Tucson, AZ 85724 > > algranth@email.arizona.edu > Tel: 520.626.4415 Fax: 520.626.2097 > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 12 Date: Wed, 17 Jul 2013 16:34:05 +0000 From: joelle weaver Subject: RE: [Histonet] microtome aligner To: "Grantham, Andrea L - (algranth)" Cc: HistoNet Message-ID: Content-Type: text/plain; charset="iso-8859-1" Yes you can certainly align the old fashioned way easy enough, but I find these are very handy and do save some time. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: algranth@email.arizona.edu > CC: histonet@lists.utsouthwestern.edu > Date: Wed, 17 Jul 2013 15:14:16 +0000 > Subject: Re: [Histonet] microtome aligner > > They do work. I saw one at NSH some years ago and came home and had the guys in our shop make one for me exactly like the one I saw and it cost next to nothing. We bought the little round level at home depot. > > Andi > > > > > Andrea Grantham, HT (ASCP) > Senior Research Specialist > University of Arizona > Cellular and Molecular Medicine > Histology Service Laboratory > P.O.Box 245044 > Tucson, AZ 85724 > > algranth@email.arizona.edu > Tel: 520.626.4415 Fax: 520.626.2097 > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 13 Date: Wed, 17 Jul 2013 16:35:29 +0000 From: Vanessa Avalos Subject: [Histonet] Lab Equipment Repair in AZ?? To: "histonet-request@lists.utsouthwestern.edu" , "histonet@lists.utsouthwestern.edu" Message-ID: <6a98ea2a7359418fa1bcbb0d57a879b7@BY2PR07MB217.namprd07.prod.outlook.com> Content-Type: text/plain; charset="us-ascii" We recently found out that our equipment repair tech has retired. For our PM's and small repairs of our non contracted lab equipment we have used a local company for years. Sad to see him go. Does any one have any other local medical lab equipment techs/companies out in AZ (Phoenix) they would recommend? I am not looking for reps to offer warranties, only recommendations at this time. Thanks in advance! V.Avalos ADS, INC Fax:602-277-2134 ------------------------------ Message: 14 Date: Wed, 17 Jul 2013 16:39:31 +0000 From: Leila Etemadi Subject: [Histonet] My perfusion problem To: "histonet@lists.utsouthwestern.edu" Message-ID: <52551199-3F4A-451B-A02E-0D6B06529AA6@med.lu.se> Content-Type: text/plain; charset="utf-8" Hi, I am desperately looking for an answer to my problem in perfusion procedure. What is my problem? To explain that I thought first I may clear what is my usual routin. I used to perfuse rat's brain through this procedure: 1- Anesthetise animal with sufficient amount of pentobarbital. 2- Clamp the vessel lying beside vertebra in the back, and then using a perfusion pump with an 17 or 18 gauge blunted needle inserted into the ascending aorta. 3- Washout blood with 0.9% salin ( room temperature), 150-200 ml, 76 rpm speed ( usually I wait to see the out put solution from the heart is not looking so red/ blood look like). 4- Switch to the 4% cold paraformaldehyde ( with buffer, 7.4 pH) ( on ice cold), 150-200 ml, 46 rpm speed. ( some times I can see the formalin dance but not always !). 5- after using this amount of PFA normally the neck part is quite stiff, then animal will be decapitated. I extract the brain right away, postfix in PFA over the night ( 4??c in the refrigerator). 6- Move to the sucrose 20%. When I saw brain is sinking in the solution ( which normally it happens in one and half day), I freeze the brain on dry ice quickly. The results of this procedure is good enough histology after dissection with cryostat ( 12-20??m). For my recent project I need brain and spinal cord both so basically I skip clamping the back along vertebra to circulate solution through whole body ( I didn't change any other steps, so I do exactly whatever I mentioned above except for spinal cord post fixation in PFA is about 2 hours). After sectioning ( 16??m), I've got a proper tissue from spinal cord but when it comes to the brain result is quite different !!, when I replaced my sections on the slide ( with plus charge), suddenly a lot of hole began to shaped in the tissue which practically ruined the entire piece..!!!, First I had no clue for what I could see but then I noticed during post fixation procedure, when I transfer brain from PFA solution to the sucrose, brain is sinking right away ( it is not floating at all, as it suppose to do..) !!!!! So I've run another perfusion procedure but this time when I reached to the washing out by cold PFA I didn't decreased the pump speed ( as normally I decrease it from 76 rpm to 46 rpm, this time I run whole procedure with the speed of 76 rpm), on the other hand I used more PFA solution ( about 350 ml PFA). After extracting brain I noticed brain was looking dried up. during post fixation and cryoprotection procedure (sucrose solution), sinking was looking normal, but after sectioning of course brain tissue was destroyed again!!! Now, I need your expert advices for my problem. I apologise for my naivety on this subject in advance. Humbly appreciate for your great time and attention in advance. I severely looking forward to your help and valuable tips. With the best, Leila Leila Etemadi M.Sc., Ph.D Candidate Neuronano Research Center (NRC) Lund University, BMC F10 Sweden Tel: +46-46-2221503 E-mail: Leila.Etemadi@med.lu.se Sent from my iPad ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 116, Issue 16 ***************************************** Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From ewj <@t> pigsqq.org Wed Jul 17 13:01:47 2013 From: ewj <@t> pigsqq.org (ewj@pigsqq.org) Date: Wed Jul 17 13:01:57 2013 Subject: [Histonet] non-xylene processing Message-ID: <20130717180147.6199.qmail@station195.com> We now have a no-xylene method start to finish We have a Midea (Mei Di) 800W manual dial microwave oven that we have rigged with a K-type metal shielded thermocouple that goes in through a slightly enlarged vent hole in the internal guide housing. The internal length of the thermocouple wire is wrapped with heavy duty aluminum foil to block microwaves from creating silly currents in the thermocouple wire. PT100 RTD's are great for water baths but microwaves zap them in seconds. The hysteresis temp controller switches a 12v relay which drives a 10A 240V relay that supplies power to a plain socket for the microwave oven. The microwave oven is a manual dial type so just cutting the power turns it off and on quite effectively. A low power (% time) setting is enough. High settings give too much overshoot. The 12V DC circuit allows us to eliminate problems with the back EMF from the MW oven and 240V relay. We have a diode that blocks the DC back EMF. Cut in well fixed tissues - fixed in formalin or Bouins. Microwave Cassettes in 100% Isopropanol at 60C - 10 min Repeat with fresh IsopOH another 10 min. Put cassettes in a fired clay earthenware pot - Pour in paraffin heated to 75C in a metal teapot. Microwave 10 min at 80C Pour out paraffin into open metal pot. Repeat above 10 min. Pour out paraffin to metal pot. Take cassettes to the embedding station, Embed, ice, trim, section. Bake 20 min on slide warmer at 60C We dewax with 95C tap water heated in a tank. A PT100 RTD drives a hysteresis controller. Also we use 12 V relay switched by the controller to drive the 240V relay that switches the heating element on and off. We have an electric solenoid valve and a waterproof switch to control the water flow when we need the hot water. The hot water flows into a 1 L pot with silicone rubber handles. We add 20 gm of a dishwasher detergent powder made in Shanghai. (It's similar to Cascade. We dont like Finish very much as sold in China). We put in the slide racks after the water and detergent are in the pot. We treat for one minute, remove the slides, pour out the water and repeat for another 1 minute soak. Next comes a 30 sec soak in clear hot tap water and a 1 min rinse in plain running tap water. Then 0.5% Periodic acid 10 min Tap water rinse 1 min Distilled water 1 min Harris Hematoxylin 4 min Rinse tap water 1 min 1% HCl in 70% EtOH 30 sec Rinse and blue in tap water 1 min Eosin/Biebrich Scarlet 30 sec Rinse tap water 1 min Rinse Distilled Water 30 sec Dry with hair dryer Coverslip. Our mounting medium contains xylene as a clearing agent/diluent. That's the only xylene in the normal runs. We like this method because it is quick, environmentally friendly, and people friendly and it produces very lovely slides that we can read and photograph for our reports. A major part of our work is diagnosis of pig diseases, so the ability to give same-day histopath results on formalin fixed samples is valuable to our customers. We do still use xylene to dewax formalin fixed paraffin block sections that are used for PCR presently, but we think we can use hot soapy water for that also with some larger tubes. We heat the teapot on an induction stove. We have that rigged for thermal control also and a PT100 in through the lid in the teapot. It is much trickier controlling the induction stove, because they have many built-in safety features and you can't just turn one off and on again at the mains outlet. What you have to do is solder in leads to a relay across the on-off push button, and rig the relay for momentary contact by including a capacitor that is charged for "on" and discharged for "off", with a momentary contact at each time faking a key press. This lets us heat our paraffin teapot accurately with induction stove efficiency and automatic temp control. E. Wayne Johnson Enable Ag Tech Beijing From mmooreht <@t> yahoo.com Wed Jul 17 14:27:04 2013 From: mmooreht <@t> yahoo.com (Michelle Moore) Date: Wed Jul 17 14:27:08 2013 Subject: [Histonet] non-xylene processing In-Reply-To: <20130717180147.6199.qmail@station195.com> References: <20130717180147.6199.qmail@station195.com> Message-ID: <1374089224.11661.YahooMailNeo@web125106.mail.ne1.yahoo.com> We also de-wax slides in a similar way as the procedure listed below. We also use a waterbath and dish soap (Dawn)/rinse agent method. (Rinse agent (Cascade) helps with water tension.) The key?is water temp. it has to be over 90 C in order for it to work. Obviously if you de-wax non-chemical you do not have the alcohols on the stain line either = huge cost savings all the way around, not to mention the healthier work area! Michelle Moore SRMC St. Thomas, USVI ________________________________ From: "ewj@pigsqq.org" To: histonet@lists.utsouthwestern.edu Sent: Wednesday, July 17, 2013 1:01 PM Subject: [Histonet] non-xylene processing We now have a no-xylene method start to finish We have a Midea (Mei Di) 800W manual dial microwave oven that we have rigged with a K-type metal shielded thermocouple that goes in through a slightly enlarged vent hole in the internal guide housing.? The internal length of the thermocouple wire is wrapped with heavy duty aluminum foil to block microwaves from creating silly currents in the thermocouple wire.? PT100 RTD's are great for water baths but microwaves zap them in seconds. The hysteresis temp controller switches a 12v relay which drives a 10A 240V relay that supplies power to a plain socket for the microwave oven. The microwave oven is a manual dial type so just cutting the power turns it off and on quite effectively.? A low power (% time) setting is enough.? High settings give too much overshoot. The 12V DC circuit allows us to eliminate problems with the back EMF from the MW oven and 240V relay.? We have a diode that blocks the DC back EMF. Cut in well fixed tissues - fixed in formalin or Bouins. Microwave Cassettes in 100% Isopropanol at 60C - 10 min Repeat with fresh IsopOH another 10 min. Put cassettes in a fired clay earthenware pot - Pour in paraffin heated? to 75C in a metal teapot. Microwave 10 min at 80C Pour out paraffin into open metal pot. Repeat above 10 min. Pour out paraffin to metal pot. Take cassettes to the embedding station, Embed, ice, trim, section. Bake 20 min on slide warmer at 60C We dewax with 95C tap water heated in a tank. A PT100 RTD drives a hysteresis controller. Also we use 12 V relay switched by the controller to drive the 240V relay that switches the heating element on and off. We have an electric solenoid valve and a waterproof switch to control the water flow when we need the hot water. The hot water flows into a 1 L pot with silicone rubber handles. We add 20 gm of a dishwasher detergent powder made in Shanghai. (It's similar to Cascade.? We dont like Finish very much as sold in China). We put in the slide racks after the water and detergent are in the pot. We treat for one minute, remove the slides, pour out the water and repeat for another 1 minute soak. Next comes a 30 sec soak in clear hot tap water and a 1 min rinse in plain running tap water. Then 0.5% Periodic acid 10 min Tap water rinse 1 min Distilled water 1 min Harris Hematoxylin 4 min Rinse tap water 1 min 1% HCl in 70% EtOH 30 sec Rinse and blue in tap water 1 min Eosin/Biebrich Scarlet 30 sec Rinse tap water 1 min Rinse Distilled Water 30 sec Dry with hair dryer Coverslip. Our mounting medium contains xylene as a clearing agent/diluent. That's the only xylene in the normal runs. We like this method because it is quick, environmentally friendly, and people friendly and it produces very lovely slides that we can read and photograph for our reports. A major part of our work is diagnosis of pig diseases, so the ability to give same-day histopath results on formalin fixed samples is valuable to our customers. We do still use xylene to dewax formalin fixed paraffin block sections that are used for PCR presently, but we think we can use hot soapy water for that also with some larger tubes. We heat the teapot on an induction stove.? We have that rigged for thermal control also and a PT100 in through the lid in the teapot.? It is much trickier controlling the induction stove, because they have many built-in safety features and you can't just turn one off and on again at the mains outlet.? What you have to do is solder in leads to a relay across the on-off push button, and rig the relay for momentary contact by including a capacitor that is charged for "on" and discharged for "off", with a momentary contact at each time faking a key press. This lets us heat our paraffin teapot accurately with induction stove efficiency and automatic temp control. E. Wayne Johnson Enable Ag Tech Beijing _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ewj <@t> pigsqq.org Wed Jul 17 16:11:35 2013 From: ewj <@t> pigsqq.org (ewj@pigsqq.org) Date: Wed Jul 17 16:11:43 2013 Subject: =?utf-8?B?UmU6IFtIaXN0b25ldF0gbm9uLXh5bGVuZSBwcm9jZXNzaW5n?= In-Reply-To: <1374089224.11661.YahooMailNeo@web125106.mail.ne1.yahoo.com> References: <20130717180147.6199.qmail@station195.com> <1374089224.11661.YahooMailNeo@web125106.mail.ne1.yahoo.com> Message-ID: <20130717211135.11711.qmail@station195.com> We used Dawn until we ran out. Dawn can work. It is hard to get here. We used calgon with the dawn because the water is so hard. > -------Original Message------- > From: Michelle Moore > To: ewj@pigsqq.org , histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] non-xylene processing > Sent: Jul 18 '13 03:27 > > We also de-wax slides in a similar way as the procedure listed below. We > also use a waterbath and dish soap (Dawn)/rinse agent method. (Rinse agent > (Cascade) helps with water tension.) The key is water temp. it has to be > over 90 C in order for it to work. Obviously if you de-wax non-chemical you > do not have the alcohols on the stain line either = huge cost savings all > the way around, not to mention the healthier work area! > Michelle Moore > SRMC > St. Thomas, USVI > > > FROM: "ewj@pigsqq.org" > TO: histonet@lists.utsouthwestern.edu > SENT: Wednesday, July 17, 2013 1:01 PM > SUBJECT: [Histonet] non-xylene processing > > > We now have a no-xylene method start to finish > > We have a Midea (Mei Di) 800W manual dial microwave oven > that we have rigged with a K-type metal shielded thermocouple > that goes in through a slightly enlarged vent hole in the internal > guide housing. The internal length of the thermocouple wire is > wrapped with heavy duty aluminum foil to block microwaves from > creating silly currents in the thermocouple wire. PT100 RTD's > are great for water baths but microwaves zap them in seconds. > > The hysteresis temp controller switches a 12v relay which drives > a 10A 240V relay that supplies power to a plain socket for the microwave > oven. > The microwave oven is a manual dial type so just cutting the power > turns it off and on quite effectively. A low power (% time) setting > is enough. High settings give too much overshoot. > The 12V DC circuit allows us to eliminate problems with the > back EMF from the MW oven and 240V relay. We > have a diode that blocks the DC back EMF. > > Cut in well fixed tissues - fixed in formalin or Bouins. > Microwave Cassettes in 100% Isopropanol at 60C - 10 min > Repeat with fresh IsopOH another 10 min. > > Put cassettes in a fired clay earthenware pot - > Pour in paraffin heated to 75C in a metal teapot. > Microwave 10 min at 80C > Pour out paraffin into open metal pot. > Repeat above 10 min. > Pour out paraffin to metal pot. > > Take cassettes to the embedding station, > Embed, ice, trim, section. > Bake 20 min on slide warmer at 60C > > We dewax with 95C tap water heated in a tank. > A PT100 RTD drives a hysteresis controller. > Also we use 12 V relay switched by the controller to drive the 240V > relay that switches the heating element on and off. > > We have an electric solenoid valve and a waterproof switch to control the > water flow when we need the hot water. > > The hot water flows into a 1 L pot with silicone rubber handles. > We add 20 gm of a dishwasher detergent powder made in Shanghai. > (It's similar to Cascade. We dont like Finish very much as sold in China). > > We put in the slide racks after the water and detergent are in the pot. > We treat for one minute, remove the slides, pour out the > water and repeat for another 1 minute soak. > Next comes a 30 sec soak in clear hot tap water > and a 1 min rinse in plain running tap water. > > Then > 0.5% Periodic acid 10 min > Tap water rinse 1 min > Distilled water 1 min > Harris Hematoxylin 4 min > Rinse tap water 1 min > 1% HCl in 70% EtOH 30 sec > Rinse and blue in tap water 1 min > Eosin/Biebrich Scarlet 30 sec > Rinse tap water 1 min > Rinse Distilled Water 30 sec > Dry with hair dryer > Coverslip. > > Our mounting medium contains xylene as a clearing agent/diluent. > That's the only xylene in the normal runs. > > We like this method because it is quick, environmentally friendly, and > people friendly > and it produces very lovely slides that we can read and photograph for our > reports. > > A major part of our work is diagnosis of pig diseases, so the ability to > give same-day > histopath results on formalin fixed samples is valuable to our customers. > > We do still use xylene to dewax formalin fixed paraffin block sections that > are used for PCR presently, > but we think we can use hot soapy water for that also with some larger > tubes. > > We heat the teapot on an induction stove. We have that rigged for thermal > control also > and a PT100 in through the lid in the teapot. It is much trickier > controlling the induction > stove, because they have many built-in safety features and you can't just > turn one off and > on again at the mains outlet. What you have to do is solder in leads to a > relay across the > on-off push button, and rig the relay for momentary contact by including a > capacitor that is charged > for "on" and discharged for "off", with a momentary contact at each time > faking a key press. > This lets us heat our paraffin teapot accurately with induction stove > efficiency and automatic temp control. > > E. Wayne Johnson > Enable Ag Tech > Beijing > > _______________________________________________ > Histonet mailing list > [LINK: mailto:Histonet@lists.utsouthwestern.edu] > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tfountain <@t> dsmanitoba.ca Wed Jul 17 16:29:25 2013 From: tfountain <@t> dsmanitoba.ca (Tiana Baskin) Date: Wed Jul 17 16:29:30 2013 Subject: [Histonet] Formalin Neutralization Message-ID: Hi Histonetters, We are looking to other labs for information and suggestions for formalin neutralization. What products do you use and do they work effectively? Thanks for your input!! Tiana Baskin -------------- next part -------------- This email and/or any documents in this transmission is intended for the addressee(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original. Ce courriel et tout document dans cette transmission est destin? ? la personne ou aux personnes ? qui il est adress?. Il peut contenir des informations privil?gi?es ou confidentielles. Toute utilisation, divulgation, distribution, copie, ou diffusion non autoris?e est strictement d?fendue. Si vous n'?tes pas le destinataire de ce message, veuillez en informer l'exp?diteur imm?diatement et lui remettre l'original. From lpwenk <@t> sbcglobal.net Thu Jul 18 05:14:16 2013 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Thu Jul 18 05:14:29 2013 Subject: [Histonet] RE: Competency for Anatomic and Clinical Pathology In-Reply-To: References: Message-ID: On Wednesday (yesterday), there was a CAP teleconference on the to-be-updated (to be posted end of July 2013) AP checklist. Someone asked a question about this, mentioning that "someone" from CAP had said competency assessment does not apply to histology. The reply from the presenter and the CAP "office" people who could also respond was that competency assess does apply to histology, but that some of the 6 components that have to be checked for may not apply for some or most of the histotech jobs. So if a component of competency doesn't apply, then it doesn't have to be evaluated. Below is part of the standard: GEN.55500 Competency Assessment Phase II The competency of each person to perform his/her assigned duties is assessed. NOTE: The competency of each person to perform the duties assigned must be assessed following training before the person performs patient testing.Thereafter, during the first year of an individual's duties, competency must be assessed at least semiannually. After an individual has performed his/her duties for one year, competency must be assessed annually. Retraining and reassessment of employee competency must occur when problems are identified with employee performance. Elements of competency assessment include but are not limited to: 1. Direct observations of routine patient test performance, including, as applicable, patient identification and preparation; and specimen collection, handling, processing and testing 2. Monitoring the recording and reporting of test results, including, as applicable, reporting critical results 3. Review of intermediate test results or worksheets, quality control records, proficiency testing results, and preventive maintenance records 4. Direct observation of performance of instrument maintenance and function checks 5. Assessment of test performance through testing previously analyzed specimens, internal blind testing samples or external proficiency testing samples; and 6. Evaluation of problem-solving skills These are the 6 components, all of which must be assessed for every task done by histotech - except if it doesn't apply. So, for example, if you are assessing the competency of sectioning, then #2 - reporting of critical values, and #5 - blind testing samples - doesn't apply, so you don't need to assess via #2 and #5. But you would have to assess the person microtoming via the other 4 types of assessment. (But if you are participating in HistoQIP, microtomy is being assess via external proficiency testing sample, so if some of your people's slides were evaluated, you actually have #5 covered for microtomy). So every aspect of the histotech's job (and the lab assistant) must be assessed by as many of the 6 elements above as apply to each task. CAP says we must assess competency, Joint Commission says we must assess competency, and CLIA says we must assess competency, and the 6 elements come from CLIA. So we must assess competency. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 The opinions expressed are mine, and do not reflect on my hospital. -----Original Message----- From: Michelle Lamphere Sent: Wednesday, July 17, 2013 9:10 AM To: 'histonet@lists.utsouthwestern.edu' Cc: Elma Cortinas Subject: [Histonet] RE: Competency for Anatomic and Clinical Pathology I think a few people might find this interesting.... I recently attended a class about Competency Assessments in the lab. The class was given by Ken Byrd (fairly certain that is how you spell his name), a Senior Inspector at CAP. When this particular question came up, I asked him to give examples of how the histology lab was supposed to use the 6 elements to assess competency. He informed the entire class that the competency assessment question with the six elements did not apply to the histology lab because histology did not report test results. It is the one question on the Gen Lab checklist that did not apply to ANP. Kinda shocking, I know. It does not mean that we scrapped our entire competency program, we simply removed some of the six elements. Michelle Lamphere Senior Tech, Histology Anatomic Pathology Children's Medical Center O: 214.456.2318 | Fax: 214.456.0779 E: michelle.lamphere@childrens.com 1935 Medical District Drive | B1.06 | Dallas, Texas 75235 Message: 9 Date: Tue, 16 Jul 2013 07:14:08 -0400 From: "Histology" Subject: [Histonet] Competency for Anatomic and Clinical Pathology at To: Message-ID: <9C0B151B30AC5F4ABFD1B69D915F84CF33E6D4@dc01.DominionPath.local> Content-Type: text/plain; charset="us-ascii" Hi, I would love to see your competency spreadsheet for histology. We just finished our first CAP inspection and got a deficiency here. He said the direct observation was great, but that we need to have all 6 elements. I am having trouble trying to come up with a way to evaluate some of these and would love any help. Thanks in advance, Mehndi Helgren Dominion Pathology Laboratories 733 Boush St. Suite 200 Norfolk, VA 23510 757-664-7901 histo@pathlab.us Please consider the environment before printing this e-mail. This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Thu Jul 18 07:21:25 2013 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Thu Jul 18 07:21:30 2013 Subject: [Histonet] RE: Competency for Anatomic and Clinical Pathology In-Reply-To: References: , Message-ID: Thanks Peggy. I appreciate this, I hate to think we were taking a step back... Joelle Weaver MAOM, HTL (ASCP) QIHC > From: lpwenk@sbcglobal.net > To: MICHELLE.LAMPHERE@childrens.com; histonet@lists.utsouthwestern.edu > Date: Thu, 18 Jul 2013 06:14:16 -0400 > Subject: Re: [Histonet] RE: Competency for Anatomic and Clinical Pathology > CC: ELMA.Cortinas@childrens.com > > On Wednesday (yesterday), there was a CAP teleconference on the > to-be-updated (to be posted end of July 2013) AP checklist. > > Someone asked a question about this, mentioning that "someone" from CAP had > said competency assessment does not apply to histology. > > The reply from the presenter and the CAP "office" people who could also > respond was that competency assess does apply to histology, but that some of > the 6 components that have to be checked for may not apply for some or most > of the histotech jobs. So if a component of competency doesn't apply, then > it doesn't have to be evaluated. > > Below is part of the standard: > > GEN.55500 Competency Assessment Phase II > The competency of each person to perform his/her assigned duties is > assessed. > NOTE: The competency of each person to perform the duties assigned must be > assessed following > training before the person performs patient testing.Thereafter, during the > first year of an individual's > duties, competency must be assessed at least semiannually. After an > individual has performed > his/her duties for one year, competency must be assessed annually. > Retraining and reassessment > of employee competency must occur when problems are identified with employee > performance. > Elements of competency assessment include but are not limited to: > 1. Direct observations of routine patient test performance, including, as > applicable, patient > identification and preparation; and specimen collection, handling, > processing and testing > 2. Monitoring the recording and reporting of test results, including, as > applicable, reporting > critical results > 3. Review of intermediate test results or worksheets, quality control > records, proficiency > testing results, and preventive maintenance records > 4. Direct observation of performance of instrument maintenance and function > checks > 5. Assessment of test performance through testing previously analyzed > specimens, internal > blind testing samples or external proficiency testing samples; and > 6. Evaluation of problem-solving skills > > These are the 6 components, all of which must be assessed for every task > done by histotech - except if it doesn't apply. So, for example, if you are > assessing the competency of sectioning, then #2 - reporting of critical > values, and #5 - blind testing samples - doesn't apply, so you don't need to > assess via #2 and #5. But you would have to assess the person microtoming > via the other 4 types of assessment. (But if you are participating in > HistoQIP, microtomy is being assess via external proficiency testing sample, > so if some of your people's slides were evaluated, you actually have #5 > covered for microtomy). > > So every aspect of the histotech's job (and the lab assistant) must be > assessed by as many of the 6 elements above as apply to each task. > > CAP says we must assess competency, Joint Commission says we must assess > competency, and CLIA says we must assess competency, and the 6 elements come > from CLIA. So we must assess competency. > > Peggy A. Wenk, HTL(ASCP)SLS > William Beaumont Hospital > Royal Oak, MI 48073 > > The opinions expressed are mine, and do not reflect on my hospital. > > -----Original Message----- > From: Michelle Lamphere > Sent: Wednesday, July 17, 2013 9:10 AM > To: 'histonet@lists.utsouthwestern.edu' > Cc: Elma Cortinas > Subject: [Histonet] RE: Competency for Anatomic and Clinical Pathology > > I think a few people might find this interesting.... > > I recently attended a class about Competency Assessments in the lab. The > class was given by Ken Byrd (fairly certain that is how you spell his name), > a Senior Inspector at CAP. When this particular question came up, I asked > him to give examples of how the histology lab was supposed to use the 6 > elements to assess competency. He informed the entire class that the > competency assessment question with the six elements did not apply to the > histology lab because histology did not report test results. It is the one > question on the Gen Lab checklist that did not apply to ANP. Kinda > shocking, I know. > > It does not mean that we scrapped our entire competency program, we simply > removed some of the six elements. > > > > Michelle Lamphere > Senior Tech, Histology > Anatomic Pathology > Children's Medical Center > O: 214.456.2318 | Fax: 214.456.0779 > E: michelle.lamphere@childrens.com > 1935 Medical District Drive | B1.06 | Dallas, Texas 75235 > > > > Message: 9 > Date: Tue, 16 Jul 2013 07:14:08 -0400 > From: "Histology" > Subject: [Histonet] Competency for Anatomic and Clinical Pathology at > To: > Message-ID: > <9C0B151B30AC5F4ABFD1B69D915F84CF33E6D4@dc01.DominionPath.local> > Content-Type: text/plain; charset="us-ascii" > > Hi, > > > > I would love to see your competency spreadsheet for histology. We just > finished our first CAP inspection and got a deficiency here. He said the > direct observation was great, but that we need to have all 6 elements. I am > having trouble trying to come up with a way to evaluate some of these and > would love any help. > > > > Thanks in advance, > > > > Mehndi Helgren > > Dominion Pathology Laboratories > > 733 Boush St. > > Suite 200 > > Norfolk, VA 23510 > > 757-664-7901 > > histo@pathlab.us > > > Please consider the environment before printing this e-mail. > > This e-mail, facsimile, or letter and any files or attachments transmitted > with it contains > information that is confidential and privileged. This information is > intended only for the > use of the individual(s) and entity(ies) to whom it is addressed. If you are > the intended > recipient, further disclosures are prohibited without proper authorization. > If you are not > the intended recipient, any disclosure, copying, printing, or use of this > information is > strictly prohibited and possibly a violation of federal or state law and > regulations. If you > have received this information in error, please notify Children's Medical > Center Dallas > immediately at 214-456-4444 or via e-mail at privacy@childrens.com. > Children's Medical > Center Dallas and its affiliates hereby claim all applicable privileges > related to this > information. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TMcNemar <@t> lmhealth.org Thu Jul 18 07:57:46 2013 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Thu Jul 18 07:57:39 2013 Subject: [Histonet] Microtome advise... Message-ID: Hello all. I am preparing my budget and am seeking recommendations on automated microtomes. I am specifically interested in brands other than Leica (I already have two). I would appreciate any thoughts you may have. Thanks. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org ________________________________ This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From b-frederick <@t> northwestern.edu Thu Jul 18 08:25:52 2013 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Thu Jul 18 08:25:58 2013 Subject: [Histonet] RE: Competency for Anatomic and Clinical Pathology In-Reply-To: References: Message-ID: <62C639732D3F274DACED033EBDF6ADAF20D4AD85@evcspmbx2.ads.northwestern.edu> We do the blinded sample part. Our manager has us look at an H&E stained slide and we identify the tissue (basic histo) and we are asked what stain is appropriate for the tissue (special stain or IHC) Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk Sent: Thursday, July 18, 2013 5:14 AM To: Michelle Lamphere; histonet@lists.utsouthwestern.edu Cc: Elma Cortinas Subject: Re: [Histonet] RE: Competency for Anatomic and Clinical Pathology On Wednesday (yesterday), there was a CAP teleconference on the to-be-updated (to be posted end of July 2013) AP checklist. Someone asked a question about this, mentioning that "someone" from CAP had said competency assessment does not apply to histology. The reply from the presenter and the CAP "office" people who could also respond was that competency assess does apply to histology, but that some of the 6 components that have to be checked for may not apply for some or most of the histotech jobs. So if a component of competency doesn't apply, then it doesn't have to be evaluated. Below is part of the standard: GEN.55500 Competency Assessment Phase II The competency of each person to perform his/her assigned duties is assessed. NOTE: The competency of each person to perform the duties assigned must be assessed following training before the person performs patient testing.Thereafter, during the first year of an individual's duties, competency must be assessed at least semiannually. After an individual has performed his/her duties for one year, competency must be assessed annually. Retraining and reassessment of employee competency must occur when problems are identified with employee performance. Elements of competency assessment include but are not limited to: 1. Direct observations of routine patient test performance, including, as applicable, patient identification and preparation; and specimen collection, handling, processing and testing 2. Monitoring the recording and reporting of test results, including, as applicable, reporting critical results 3. Review of intermediate test results or worksheets, quality control records, proficiency testing results, and preventive maintenance records 4. Direct observation of performance of instrument maintenance and function checks 5. Assessment of test performance through testing previously analyzed specimens, internal blind testing samples or external proficiency testing samples; and 6. Evaluation of problem-solving skills These are the 6 components, all of which must be assessed for every task done by histotech - except if it doesn't apply. So, for example, if you are assessing the competency of sectioning, then #2 - reporting of critical values, and #5 - blind testing samples - doesn't apply, so you don't need to assess via #2 and #5. But you would have to assess the person microtoming via the other 4 types of assessment. (But if you are participating in HistoQIP, microtomy is being assess via external proficiency testing sample, so if some of your people's slides were evaluated, you actually have #5 covered for microtomy). So every aspect of the histotech's job (and the lab assistant) must be assessed by as many of the 6 elements above as apply to each task. CAP says we must assess competency, Joint Commission says we must assess competency, and CLIA says we must assess competency, and the 6 elements come from CLIA. So we must assess competency. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 The opinions expressed are mine, and do not reflect on my hospital. -----Original Message----- From: Michelle Lamphere Sent: Wednesday, July 17, 2013 9:10 AM To: 'histonet@lists.utsouthwestern.edu' Cc: Elma Cortinas Subject: [Histonet] RE: Competency for Anatomic and Clinical Pathology I think a few people might find this interesting.... I recently attended a class about Competency Assessments in the lab. The class was given by Ken Byrd (fairly certain that is how you spell his name), a Senior Inspector at CAP. When this particular question came up, I asked him to give examples of how the histology lab was supposed to use the 6 elements to assess competency. He informed the entire class that the competency assessment question with the six elements did not apply to the histology lab because histology did not report test results. It is the one question on the Gen Lab checklist that did not apply to ANP. Kinda shocking, I know. It does not mean that we scrapped our entire competency program, we simply removed some of the six elements. Michelle Lamphere Senior Tech, Histology Anatomic Pathology Children's Medical Center O: 214.456.2318 | Fax: 214.456.0779 E: michelle.lamphere@childrens.com 1935 Medical District Drive | B1.06 | Dallas, Texas 75235 Message: 9 Date: Tue, 16 Jul 2013 07:14:08 -0400 From: "Histology" Subject: [Histonet] Competency for Anatomic and Clinical Pathology at To: Message-ID: <9C0B151B30AC5F4ABFD1B69D915F84CF33E6D4@dc01.DominionPath.local> Content-Type: text/plain; charset="us-ascii" Hi, I would love to see your competency spreadsheet for histology. We just finished our first CAP inspection and got a deficiency here. He said the direct observation was great, but that we need to have all 6 elements. I am having trouble trying to come up with a way to evaluate some of these and would love any help. Thanks in advance, Mehndi Helgren Dominion Pathology Laboratories 733 Boush St. Suite 200 Norfolk, VA 23510 757-664-7901 histo@pathlab.us Please consider the environment before printing this e-mail. This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ratliffjack <@t> hotmail.com Thu Jul 18 08:55:01 2013 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Thu Jul 18 08:55:11 2013 Subject: [Histonet] Microtome advise... In-Reply-To: References: Message-ID: <09C21F00-273E-464C-B8F5-A6D97C4F3A53@hotmail.com> How about the new Sakura with the auto orientation feature! I saw it on display at the Missouri Society for Histotechnology meeting and it was quite impressive! Of course if you have the budget, there's alway the non-contact laser microtome from Rowiak! I have seen and used that unit to cut fresh soft tissues (brain) and resin embedded bone and biomaterial implants! Jack On Jul 18, 2013, at 7:57 AM, Tom McNemar wrote: > Hello all. I am preparing my budget and am seeking recommendations on automated microtomes. I am specifically interested in brands other than Leica (I already have two). I would appreciate any thoughts you may have. Thanks. > > Tom McNemar, HT(ASCP) > Histology Co-ordinator > Licking Memorial Health Systems > (740) 348-4163 > (740) 348-4166 > tmcnemar@lmhealth.org > www.LMHealth.org > > ________________________________ > This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From LSebree <@t> uwhealth.org Thu Jul 18 08:58:43 2013 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Thu Jul 18 08:58:49 2013 Subject: [Histonet] Hepatocyte Nuclear Factor (HNF-1B) Message-ID: <77DD817201982748BC67D7960F2F76AF0598FA@UWHC-MBX12.uwhis.hosp.wisc.edu> Good morning all, One of our pathologists has requested this antibody and we don't have it in our inventory. So I'm asking if there are any reference labs offering HNF-1B for FFPE human tissue? Thanks for any/all responses. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 From ratliffjack <@t> hotmail.com Thu Jul 18 09:38:41 2013 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Thu Jul 18 09:38:49 2013 Subject: [Histonet] Microtome advise... In-Reply-To: <09C21F00-273E-464C-B8F5-A6D97C4F3A53@hotmail.com> References: <09C21F00-273E-464C-B8F5-A6D97C4F3A53@hotmail.com> Message-ID: BTW. I'm certain that the Sakura unit will be on display at the NSH S/C meeting in Providence, RI (20-25 Sept 2013) and I know for a fact that the laser microtome will be on display there as well because it will be demonstrated and used, along with a Leica rotary microtome for part of a workshop I am co-presenting with Bob Skinner (WS # 61 - A Detailed Examination of Working With Decalcified and Undecalcified Bone In Support of Preclinical and Clinical Research). Jack On Jul 18, 2013, at 8:55 AM, Jack Ratliff wrote: > How about the new Sakura with the auto orientation feature! I saw it on display at the Missouri Society for Histotechnology meeting and it was quite impressive! Of course if you have the budget, there's alway the non-contact laser microtome from Rowiak! I have seen and used that unit to cut fresh soft tissues (brain) and resin embedded bone and biomaterial implants! > > Jack > > > On Jul 18, 2013, at 7:57 AM, Tom McNemar wrote: > >> Hello all. I am preparing my budget and am seeking recommendations on automated microtomes. I am specifically interested in brands other than Leica (I already have two). I would appreciate any thoughts you may have. Thanks. >> >> Tom McNemar, HT(ASCP) >> Histology Co-ordinator >> Licking Memorial Health Systems >> (740) 348-4163 >> (740) 348-4166 >> tmcnemar@lmhealth.org >> www.LMHealth.org >> >> ________________________________ >> This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From akemiat3377 <@t> yahoo.com Thu Jul 18 10:32:18 2013 From: akemiat3377 <@t> yahoo.com (Akemi Allison) Date: Thu Jul 18 10:32:21 2013 Subject: [Histonet] CLIA Compliance Regulations for histology staff coverage Message-ID: <1374161538.5028.YahooMailNeo@web140601.mail.bf1.yahoo.com> Good morning to all in Histoland! ? Hopefully,?one of you?can help me with a question which was proposed to me by a fellow histologist working in a very small GI histology lab. ? Here is what information she gave me:? She is training a histology assistant in house to do all the histology technical responsibilities at the request of the GI doctors.? She said her OJT assistant is doing a great job technically, but she has not yet registered in school to finish her ?AA Degree so she can sit for the HT exam.? She has been working under her instruction for 1 year.? Her lab is California State and CLIA licensed. ? The GI doctors want the assistant to cover for her while she is on vacation. ?The pathologist who is the medical director does ?not think that CLIA Regulations allows this and wants the specimens sent out during her absence.? She needs the CLIA Regulations stating what the guidelines are so her lab is complying to regulations.? She didn?t want to call CLIA because it would send a RED FLAG up.? I also didn?t want to call them because they may ask me what the name of the lab was that was proposing this question.? ? I?told her that this was most likely?illegal, but she needs it in black and white.? Do any of you have that information or a web link to go to at your finger tips? She needs the regulations by next week. ? Thank you in advance for your help, Akemi ? Akemi Allison-Tacha, BS, HT/HTL?(ASCP) Pathology Manager Monterey Bay GI Consultants 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 (381) 375-3577? X117 Email: aallison@montereygi.com From amber.mckenzie <@t> gastrodocs.net Thu Jul 18 10:32:53 2013 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Thu Jul 18 10:32:56 2013 Subject: [Histonet] Embeding log In-Reply-To: References: Message-ID: <5A33C952BB67F4468AF1F36D739212BCBD6BBE15@JERRY.Gia.com> On the daily embedding log, do you have your embdedders initial each case they embed or do you allow them to initial the first case on the page and then draw a line down the page symbolizing they did all of those? How detailed should it be? And, you keep these for 2 years like all other paperwork? Thanks! From ignac324 <@t> gmail.com Thu Jul 18 11:28:06 2013 From: ignac324 <@t> gmail.com (Ignacio Ruz Caracuel) Date: Thu Jul 18 11:28:10 2013 Subject: [Histonet] My perfusion problem In-Reply-To: <52551199-3F4A-451B-A02E-0D6B06529AA6@med.lu.se> References: <52551199-3F4A-451B-A02E-0D6B06529AA6@med.lu.se> Message-ID: Hi! When I used to do whole body perfusion, it tooks me around 10 minutes to perfuse with 0,9% salin and another 10 minutes with PFA. (Sorry, I haven?t with me the rpm) but it was around 300 ml of each. I think you have to increase the amount of 0,9% salin you perfuse (because you have increased the circuit) to avoid clot formation. I hope you find it useful and I wish you good luck! Ignacio Ruz-Caracuel Medical Student, Histology Intern Student Faculty of Medicine, University of C?rdoba SPAIN 2013/7/17 Leila Etemadi > Hi, > > I am desperately looking for an answer to my problem in perfusion > procedure. > > What is my problem? > > To explain that I thought first I may clear what is my usual routin. > > I used to perfuse rat's brain through this procedure: > > 1- Anesthetise animal with sufficient amount of pentobarbital. > > 2- Clamp the vessel lying beside vertebra in the back, and then using a > perfusion pump with an 17 or 18 gauge blunted needle inserted into the > ascending aorta. > > 3- Washout blood with 0.9% salin ( room temperature), 150-200 ml, 76 rpm > speed ( usually I wait to see the out put solution from the heart is not > looking so red/ blood look like). > > 4- Switch to the 4% cold paraformaldehyde ( with buffer, 7.4 pH) ( on ice > cold), 150-200 ml, 46 rpm speed. ( some times I can see the formalin dance > but not always !). > > 5- after using this amount of PFA normally the neck part is quite stiff, > then animal will be decapitated. I extract the brain right away, postfix in > PFA over the night ( 4?c in the refrigerator). > > 6- Move to the sucrose 20%. When I saw brain is sinking in the solution ( > which normally it happens in one and half day), I freeze the brain on dry > ice quickly. > > The results of this procedure is good enough histology after dissection > with cryostat ( 12-20?m). > > > > For my recent project I need brain and spinal cord both so basically I > skip clamping the back > > along vertebra to circulate solution through whole body ( I didn't change > any other steps, so I do > > exactly whatever I mentioned above except for spinal cord post fixation in > PFA is about 2 hours). > > After sectioning ( 16?m), I've got a proper tissue from spinal cord but > when it comes to the > > brain result is quite different !!, when I replaced my sections on the > slide ( with plus charge), > > suddenly a lot of hole began to shaped in the tissue which practically > ruined the entire > > piece..!!!, > > First I had no clue for what I could see but then I noticed during post > fixation procedure, when I > > transfer brain from PFA solution to the sucrose, brain is sinking right > away ( it is not > > floating at all, as it suppose to do..) !!!!! > > So I've run another perfusion procedure but this time when I reached to > the washing out by cold > > PFA I didn't decreased the pump speed ( as normally I decrease it from 76 > rpm to 46 rpm, > > this time I run whole procedure with the speed of 76 rpm), on the other > hand I used more PFA > > solution ( about 350 ml PFA). After extracting brain I noticed brain was > looking dried up. > > during post fixation and cryoprotection procedure (sucrose solution), > sinking was looking normal, > > but after sectioning of course brain tissue was destroyed again!!! > > Now, I need your expert advices for my problem. I apologise for my naivety > on this subject in advance. > > > Humbly appreciate for your great time and attention in advance. I severely > looking forward to your help and valuable tips. > > > With the best, > > Leila > > > > > > > > > > Leila Etemadi > M.Sc., Ph.D Candidate > Neuronano Research Center (NRC) > Lund University, BMC F10 > Sweden > > Tel: +46-46-2221503 > E-mail: Leila.Etemadi@med.lu.se > > > > > > > > > > > > Sent from my iPad > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From joelleweaver <@t> hotmail.com Thu Jul 18 11:38:59 2013 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Thu Jul 18 11:39:02 2013 Subject: [Histonet] Embeding log In-Reply-To: <5A33C952BB67F4468AF1F36D739212BCBD6BBE15@JERRY.Gia.com> References: , <5A33C952BB67F4468AF1F36D739212BCBD6BBE15@JERRY.Gia.com> Message-ID: each- ideally electronically-, or mark blocks ( various ways), all records -for accountability and related to QC- all minimum of 2 years. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: amber.mckenzie@gastrodocs.net > To: histonet@lists.utsouthwestern.edu > Date: Thu, 18 Jul 2013 15:32:53 +0000 > Subject: [Histonet] Embeding log > > > On the daily embedding log, do you have your embdedders initial each case they embed or do you allow them to initial the first case on the page and then draw a line down the page symbolizing they did all of those? How detailed should it be? And, you keep these for 2 years like all other paperwork? Thanks! > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Karen.Heckford <@t> DignityHealth.org Thu Jul 18 12:00:25 2013 From: Karen.Heckford <@t> DignityHealth.org (Heckford, Karen - SMMC-SF) Date: Thu Jul 18 12:00:31 2013 Subject: [Histonet] Histology Video Message-ID: Does anyone know of any Histology parody videos out there on YouTube? Just curious! Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." From jqb7 <@t> cdc.gov Thu Jul 18 12:02:25 2013 From: jqb7 <@t> cdc.gov (Sanders, Jeanine (CDC/OID/NCEZID)) Date: Thu Jul 18 12:02:40 2013 Subject: [Histonet] RE: Histology Video In-Reply-To: References: Message-ID: Let's make one! Actually, I probably am not allowed to...but the rest of you could! Go for it! Jeanine H. Sanders Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 404-639-3590 jqb7@cdc.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Heckford, Karen - SMMC-SF Sent: Thursday, July 18, 2013 1:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histology Video Does anyone know of any Histology parody videos out there on YouTube? Just curious! Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tony.auge <@t> gmail.com Thu Jul 18 12:03:54 2013 From: tony.auge <@t> gmail.com (Tony Auge) Date: Thu Jul 18 12:03:58 2013 Subject: [Histonet] Microtome advise... In-Reply-To: References: Message-ID: The Thermo Shandon Finesse ME+ is my all time favorite =) Tony Auge HTL (ASCP) QIHC Cell: (651) 373-4768 Email: tony.auge@gmail.com From Diana.Harris <@t> viha.ca Thu Jul 18 12:20:21 2013 From: Diana.Harris <@t> viha.ca (Harris, Diana) Date: Thu Jul 18 12:20:34 2013 Subject: [Histonet] RE: Histology Video In-Reply-To: References: Message-ID: Thrift Lab - UF Pathology Diana Harris QC & Method Development Technologist Dept. Of Laboratory Medicine Anatomical Pathology Royal Jubilee Hospital - VIHA 250-370-8402 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Heckford, Karen - SMMC-SF Sent: Thursday, July 18, 2013 10:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histology Video Does anyone know of any Histology parody videos out there on YouTube? Just curious! Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Thu Jul 18 12:27:47 2013 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Thu Jul 18 12:27:57 2013 Subject: [Histonet] CLIA Compliance Regulations for histology staff coverage In-Reply-To: <1374161538.5028.YahooMailNeo@web140601.mail.bf1.yahoo.com> References: <1374161538.5028.YahooMailNeo@web140601.mail.bf1.yahoo.com> Message-ID: California does not have licensure for histotechs. To my knowledge California does not have any regulations concerning who can cut biopsies. There are many labs that have uncertified people working without the supervision of certified people. That being said, is this the only reason the Pathologist wants the cases sent our during her absence? He is the one that has to sign his name to the cases. From: Akemi Allison To: Histonet Date: 07/18/2013 08:34 AM Subject: [Histonet] CLIA Compliance Regulations for histology staff coverage Sent by: histonet-bounces@lists.utsouthwestern.edu Good morning to all in Histoland! Hopefully, one of you can help me with a question which was proposed to me by a fellow histologist working in a very small GI histology lab. Here is what information she gave me: She is training a histology assistant in house to do all the histology technical responsibilities at the request of the GI doctors. She said her OJT assistant is doing a great job technically, but she has not yet registered in school to finish her AA Degree so she can sit for the HT exam. She has been working under her instruction for 1 year. Her lab is California State and CLIA licensed. The GI doctors want the assistant to cover for her while she is on vacation. The pathologist who is the medical director does not think that CLIA Regulations allows this and wants the specimens sent out during her absence. She needs the CLIA Regulations stating what the guidelines are so her lab is complying to regulations. She didn?t want to call CLIA because it would send a RED FLAG up. I also didn?t want to call them because they may ask me what the name of the lab was that was proposing this question. I told her that this was most likely illegal, but she needs it in black and white. Do any of you have that information or a web link to go to at your finger tips? She needs the regulations by next week. Thank you in advance for your help, Akemi Akemi Allison-Tacha, BS, HT/HTL (ASCP) Pathology Manager Monterey Bay GI Consultants 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 (381) 375-3577 X117 Email: aallison@montereygi.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akbitting <@t> geisinger.edu Thu Jul 18 12:28:32 2013 From: akbitting <@t> geisinger.edu (Bitting, Angela K.) Date: Thu Jul 18 12:28:37 2013 Subject: [Histonet] RE: Histology Video In-Reply-To: References: Message-ID: <77F52EFAB8B1694B885E277C48FCD0F64A712225@GHSEXMBX4W8K1V.geisinger.edu> I saw a few a while back. Pretty funny. It looks like they were made by some Pathology residents and techs. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sanders, Jeanine (CDC/OID/NCEZID) Sent: Thursday, July 18, 2013 1:02 PM To: Heckford, Karen - SMMC-SF; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Histology Video Let's make one! Actually, I probably am not allowed to...but the rest of you could! Go for it! Jeanine H. Sanders Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 404-639-3590 jqb7@cdc.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Heckford, Karen - SMMC-SF Sent: Thursday, July 18, 2013 1:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histology Video Does anyone know of any Histology parody videos out there on YouTube? Just curious! Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. From akemiat3377 <@t> yahoo.com Thu Jul 18 12:32:44 2013 From: akemiat3377 <@t> yahoo.com (Akemi Allison) Date: Thu Jul 18 12:32:47 2013 Subject: [Histonet] Histology Training and Competency checklist Message-ID: <1374168764.27785.YahooMailNeo@web140603.mail.bf1.yahoo.com> Hi Again in Histoland. I have another favor to ask.? I was going to give my friend a Histology Training and Compentency Checklist, but I had it on my old computor and didn't transfer it to my new MAC.? I only have my Compentency checklist for Client Services from PhenoPath.? Would anyone like to share theirs with me? ? Thank you in advance for your assistance, Akemi ? Akemi Allison-Tacha, BS, HT/HTL?(ASCP) Pathology Manager Monterey Bay GI Consultants 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 (381) 375-3577? X117 Email: aallison@montereygi.com From contact <@t> excaliburpathology.com Thu Jul 18 12:38:30 2013 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Thu Jul 18 12:38:38 2013 Subject: [Histonet] Histology Video In-Reply-To: References: Message-ID: <1374169110.23068.YahooMailNeo@web5701.biz.mail.ne1.yahoo.com> This is one of the best I have seen: http://www.youtube.com/watch?v=Fl4L4M8m4d0 ? Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 8901 S. Santa Fe, Suite G Oklahoma City, OK 73139 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ________________________________ From: "Heckford, Karen - SMMC-SF" To: "histonet@lists.utsouthwestern.edu" Sent: Thursday, July 18, 2013 12:00 PM Subject: [Histonet] Histology Video Does anyone know of any Histology parody videos out there on YouTube?? Just curious! Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford@dignityhealth.org ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? Caution:? This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED.? The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error.? Any further review, dissemination, distribution, or copying of this message is strictly prohibited.? If you have received this communication in error, please notify us? immediately by reply email.? Thank you." _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b-frederick <@t> northwestern.edu Thu Jul 18 13:09:41 2013 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Thu Jul 18 13:09:47 2013 Subject: [Histonet] RE: Histology Video In-Reply-To: References: Message-ID: <62C639732D3F274DACED033EBDF6ADAF20D4AE82@evcspmbx2.ads.northwestern.edu> In Providence?!!!! Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Heckford, Karen - SMMC-SF Sent: Thursday, July 18, 2013 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histology Video Does anyone know of any Histology parody videos out there on YouTube? Just curious! Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Allison.Scott <@t> harrishealth.org Thu Jul 18 13:24:51 2013 From: Allison.Scott <@t> harrishealth.org (Scott, Allison D) Date: Thu Jul 18 13:24:56 2013 Subject: [Histonet] Carnoys solution Message-ID: Hello to all in histoland. Does anyone have a carnoys recipe that does not have call for chloroform? I had a recipe for one, but now I can't find it. Any and all help will be appreciated. Allison Scott HT(ASCP) Supervisor, Histology Lab LBJ Hospital Harris Health System Office: 713-566-2148 Lab: 713-566-5287 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. From brannon <@t> alliedsearchpartners.com Thu Jul 18 13:44:05 2013 From: brannon <@t> alliedsearchpartners.com (Brannon Owens) Date: Thu Jul 18 13:44:15 2013 Subject: [Histonet] Histotech openings 2nd and 3rd shifts Message-ID: ASCP certified Histotechs with IHC experience needed for a Florida laboratory. Two shifts available. Email me for full job description! Brannon Owens Recruitment Manager Allied Search Partners LinkedIn: http://www.linkedin.com/pub/brannon-owens/28/528/823 http://www.alliedsearchpartners.com T: 888.388.7571 ext. 106 F: 888.388.7572 From AHutton <@t> dh.org Thu Jul 18 13:57:09 2013 From: AHutton <@t> dh.org (Hutton, Allison) Date: Thu Jul 18 13:58:05 2013 Subject: [Histonet] voice recognition Message-ID: <4ED8C96A8F20FC4F883A92E2A0A0D64A765C@DH-MAIL01.dhorg.org> We are currently working on implementing voice recognition for grossing (and reading in the future). I understand how it all works but am having a hard time figuring it into my workflow. If there is anyone in the eastern PA area that is doing this (we are using Dragon) could you please email me offline. Thanks Allison ahutton@dh.org From cls71877 <@t> gmail.com Thu Jul 18 15:09:31 2013 From: cls71877 <@t> gmail.com (Cristi Rigazio) Date: Thu Jul 18 15:09:40 2013 Subject: [Histonet] RE: Histology Video In-Reply-To: <62C639732D3F274DACED033EBDF6ADAF20D4AE82@evcspmbx2.ads.northwestern.edu> References: <62C639732D3F274DACED033EBDF6ADAF20D4AE82@evcspmbx2.ads.northwestern.edu> Message-ID: If one is organized for NSH, count me in!! Sent from my iPhone On Jul 18, 2013, at 11:09 AM, Bernice Frederick wrote: > In Providence?!!!! > > Bernice Frederick HTL (ASCP) > Senior Research Tech > Pathology Core Facility > ECOGPCO-RL > Robert. H. Lurie Cancer Center > Northwestern University > 710 N Fairbanks Court > Olson 8-421 > Chicago,IL 60611 > 312-503-3723 > b-frederick@northwestern.edu > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Heckford, Karen - SMMC-SF > Sent: Thursday, July 18, 2013 12:00 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Histology Video > > Does anyone know of any Histology parody videos out there on YouTube? Just curious! > > Karen Heckford HT ASCP CE > Lead Histology Technician > St. Mary's Medical Center > 450 Stanyan St. > San Francisco, Ca. 94117 > 415-668-1000 ext. 6167 > karen.heckford@dignityhealth.org > Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Thu Jul 18 15:11:58 2013 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Thu Jul 18 15:12:03 2013 Subject: [Histonet] Carnoys solution In-Reply-To: References: Message-ID: Clarke Fluid is one part glacial acetic acid to 3 parts absolute alcohol. From: "Scott, Allison D" To: "histonet@lists.utsouthwestern.edu" Date: 07/18/2013 11:27 AM Subject: [Histonet] Carnoys solution Sent by: histonet-bounces@lists.utsouthwestern.edu Hello to all in histoland. Does anyone have a carnoys recipe that does not have call for chloroform? I had a recipe for one, but now I can't find it. Any and all help will be appreciated. Allison Scott HT(ASCP) Supervisor, Histology Lab LBJ Hospital Harris Health System Office: 713-566-2148 Lab: 713-566-5287 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Taylor.Clifford <@t> va.gov Thu Jul 18 15:15:58 2013 From: Taylor.Clifford <@t> va.gov (Clifford, Taylor) Date: Thu Jul 18 15:16:28 2013 Subject: [Histonet] FW: Carnoys solution In-Reply-To: <7FF33CF7105F23409F31E748D906B489A012F4A21E@R04BYNMSGB2.r04.med.va.gov> References: <7FF33CF7105F23409F31E748D906B489A012F4A21E@R04BYNMSGB2.r04.med.va.gov> Message-ID: <7FF33CF7105F23409F31E748D906B489A0133AAD13@R04BYNMSGB2.r04.med.va.gov> Clarke's fixative has been known to work as a replacement to Carnoy's, it is said to be the original "Carnoy's I", whereas the Carnoy's as it is known today with chloroform is Carnoy's II. http://stainsfile.info/StainsFile/prepare/fix/fixatives/clarke.htm Taylor CM Clifford Research Associate Albany Research Institute Albany Stratton VA Medical Center 113 Holland Avenue Albany, NY 12208 Room A610 518-626-5474 Taylor.Clifford@va.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Thursday, July 18, 2013 2:25 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Carnoys solution Hello to all in histoland. Does anyone have a carnoys recipe that does not have call for chloroform? I had a recipe for one, but now I can't find it. Any and all help will be appreciated. Allison Scott HT(ASCP) Supervisor, Histology Lab LBJ Hospital Harris Health System Office: 713-566-2148 Lab: 713-566-5287 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From one_angel_secret <@t> yahoo.com Thu Jul 18 18:05:34 2013 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Thu Jul 18 18:05:40 2013 Subject: [Histonet] CLIA Compliance Regulations for histology staff coverage In-Reply-To: References: <1374161538.5028.YahooMailNeo@web140601.mail.bf1.yahoo.com> Message-ID: <1374188734.37648.YahooMailNeo@web161602.mail.bf1.yahoo.com> CLIA only regulated labs can have uncertified or licensed personel doing task such as embedding, microtomy and standard H&E staining as CLIA does not regulate those task as Jennifer mentioned. That said, who is going to gross in the biopsies( High complexity task)?in the other persons absence? Because that is a CLIA regulated task and needs to be done by someone who meets CLIA 1489, which is the education, AS degree that includes the required biology and chemistry, or 60 hrs of education that includes the required biology and chemistry. ? http://www.mass.gov/eohhs/docs/dph/clinical-lab/clia-lab-qualifications.pdf ? ? Kim D ________________________________ From: Jennifer MacDonald To: Akemi Allison Cc: Histonet ; histonet-bounces@lists.utsouthwestern.edu Sent: Thursday, July 18, 2013 1:27 PM Subject: Re: [Histonet] CLIA Compliance Regulations for histology staff coverage California does not have licensure for histotechs.? To my knowledge California does not have any regulations concerning who can cut biopsies. There are many labs that have uncertified people working without the supervision of certified people.? That being said, is this the only reason the Pathologist wants the cases sent our during her absence?? He is the one that has to sign his name to the cases. From:? Akemi Allison To:? ? Histonet Date:? 07/18/2013 08:34 AM Subject:? ? ? ? [Histonet] CLIA Compliance Regulations for histology staff coverage Sent by:? ? ? ? histonet-bounces@lists.utsouthwestern.edu Good morning to all in Histoland! Hopefully, one of you can help me with a question which was proposed to me by a fellow histologist working in a very small GI histology lab. Here is what information she gave me:? She is training a histology assistant in house to do all the histology technical responsibilities at the request of the GI doctors.? She said her OJT assistant is doing a great job technically, but she has not yet registered in school to finish her? AA Degree so she can sit for the HT exam.? She has been working under her instruction for 1 year.? Her lab is California State and CLIA licensed. The GI doctors want the assistant to cover for her while she is on vacation.? The pathologist who is the medical director does? not think that CLIA Regulations allows this and wants the specimens sent out during her absence.? She needs the CLIA Regulations stating what the guidelines are so her lab is complying to regulations.? She didn?t want to call CLIA because it would send a RED FLAG up.? I also didn?t want to call them because they may ask me what the name of the lab was that was proposing this question.? I told her that this was most likely illegal, but she needs it in black and white.? Do any of you have that information or a web link to go to at your finger tips? She needs the regulations by next week. Thank you in advance for your help, Akemi Akemi Allison-Tacha, BS, HT/HTL (ASCP) Pathology Manager Monterey Bay GI Consultants 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 (381) 375-3577? X117 Email: aallison@montereygi.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jshea121 <@t> roadrunner.com Thu Jul 18 19:08:56 2013 From: jshea121 <@t> roadrunner.com (Shea's) Date: Thu Jul 18 19:09:02 2013 Subject: [Histonet] QA forms Message-ID: <433E37873E1A450AA37E92E44CCECAB1@JoannePC> Our state requires us to keep them for 4 years...... Before we write retention policies, we are required to look at CMS, State and CAP requirements. I have these attachment charts in my retention policy (as references). The policy states that we will retain for a minimum of __________(most astringent). Like most, if space allows, I can keep longer, however, not obligated....see attached Stated from CAP "Some state regulations as well as other federal mandates may require retention of records and/or materials for a longer time period than that specified in the CLIA 88 regulations; therefore any applicable state or federal laws should be reviewed carefully when individual laboratories develop their record retention policies." From tony.henwood <@t> health.nsw.gov.au Thu Jul 18 20:22:37 2013 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Thu Jul 18 20:23:02 2013 Subject: [Histonet] RE: Carnoys solution In-Reply-To: References: Message-ID: <6D6BD1DE8A5571489398B392A38A71579D28CEC7@xmdb04.nch.kids> Hi Allison, It will depend on what you are using the Carnoy's for. If it is for the removal of blood from smeared cytology preps then Clarke's fluid, a 3:1 mixture of ethanol and acetic acid, works very well. For fixation of tissue samples prior to processing to wax then you probably need to use the original Carnoy's recipe, though Meier's Fixative (Glacial acetic acid 25ml + 95% ethanol 475ml), with less acetic acid has been used in the past. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Friday, 19 July 2013 4:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Carnoys solution Hello to all in histoland. Does anyone have a carnoys recipe that does not have call for chloroform? I had a recipe for one, but now I can't find it. Any and all help will be appreciated. Allison Scott HT(ASCP) Supervisor, Histology Lab LBJ Hospital Harris Health System Office: 713-566-2148 Lab: 713-566-5287 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From susanbachus <@t> verizon.net Fri Jul 19 09:56:04 2013 From: susanbachus <@t> verizon.net (susanbachus@verizon.net) Date: Fri Jul 19 09:56:21 2013 Subject: [Histonet] histology humor on youtube In-Reply-To: <5A33C952BB67F4468AF1F36D739212BCBD6ADF63@JERRY.Gia.com> References: <5BD80891-C417-4913-8A0D-2F40AA47F8DB@gmail.com> <5A33C952BB67F4468AF1F36D739212BCBD6ADF63@JERRY.Gia.com> Message-ID: <4702AADF74764E35B66A71294FC98AAA@UserPC> I'm afraid I don't have the original query any more, but I just stumbled onto this video from a Pathology lab, which does include some histology. Be forewarned that there's some profanity. https://www.youtube.com/watch?v=ZL7a3_JRWQY From talulahgosh <@t> gmail.com Fri Jul 19 11:39:09 2013 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Fri Jul 19 11:39:22 2013 Subject: [Histonet] histology humor on youtube In-Reply-To: <4702AADF74764E35B66A71294FC98AAA@UserPC> References: <5BD80891-C417-4913-8A0D-2F40AA47F8DB@gmail.com> <5A33C952BB67F4468AF1F36D739212BCBD6ADF63@JERRY.Gia.com> <4702AADF74764E35B66A71294FC98AAA@UserPC> Message-ID: Hey, I just found that today too! Are you a farker?? I almost sent it to the list, but then decided someone had already done so and I missed it. I heard that the university is upset because it's gone viral, something about inappropriate behavior in the lab. NO HAVING FUN, EVER!! :) Emily "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." -Chuck Palahniuk, "Haunted" On Fri, Jul 19, 2013 at 10:56 AM, wrote: > I'm afraid I don't have the original query any more, but I just stumbled > onto this video from a Pathology lab, which does include some histology. Be > forewarned that there's some profanity. https://www.youtube.com/watch?** > v=ZL7a3_JRWQY > > > ______________________________**_________________ > Histonet mailing list > Histonet@lists.utsouthwestern.**edu > http://lists.utsouthwestern.**edu/mailman/listinfo/histonet > From susanbachus <@t> verizon.net Fri Jul 19 11:51:58 2013 From: susanbachus <@t> verizon.net (susanbachus@verizon.net) Date: Fri Jul 19 11:52:13 2013 Subject: [Histonet] histology humor on youtube In-Reply-To: References: <5BD80891-C417-4913-8A0D-2F40AA47F8DB@gmail.com> <5A33C952BB67F4468AF1F36D739212BCBD6ADF63@JERRY.Gia.com> <4702AADF74764E35B66A71294FC98AAA@UserPC> Message-ID: <1C89DD2B9F0B4B2AA2B6FAE610672E47@UserPC> Sorry, I don't even know what a farker is! This was shared on a veterinarian's FB page! I almost sent the Lady Science one, which I think is hysterical, but I was afraid it wasn't relevant enough to histology per se (all biochemistry). But I'm glad someone else did. Well, maybe these will give a creative histologist some ideas... -----Original Message----- From: Emily Sours Sent: Friday, July 19, 2013 12:39 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] histology humor on youtube Hey, I just found that today too! Are you a farker?? I almost sent it to the list, but then decided someone had already done so and I missed it. I heard that the university is upset because it's gone viral, something about inappropriate behavior in the lab. NO HAVING FUN, EVER!! :) Emily "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." -Chuck Palahniuk, "Haunted" On Fri, Jul 19, 2013 at 10:56 AM, wrote: > I'm afraid I don't have the original query any more, but I just stumbled > onto this video from a Pathology lab, which does include some histology. > Be > forewarned that there's some profanity. https://www.youtube.com/watch?** > v=ZL7a3_JRWQY > > > ______________________________**_________________ > Histonet mailing list > Histonet@lists.utsouthwestern.**edu > http://lists.utsouthwestern.**edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> gmail.com Fri Jul 19 11:56:55 2013 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Fri Jul 19 11:56:57 2013 Subject: [Histonet] Re: Carnoy solution Message-ID: Carnoy's fixative contains chloroform, and that's enough reason not to use it. In addition to its toxicity, chloroform dissolves methacrylate plastic, as I found out the hard way once when I tried to fix a specimen in Carnoy's fixative in a methacrylate container because I had nothing else. I've seen Carnoy's fixative used as a "disclosing fixative" for finding mesenteric lymph nodes in colectomy specimens. There's no excuse for this. There are a number of proprietary fixatives (such as Surgipath's O-Fix) for this purpose, or if you're allowed to brew your own, I use Davidson's fixative (3 parts water, 3 parts reagent alcohol, 2 parts 37% formaldehyde [NOT 10% NBF], 1 part glacial acetic acid.) Bob Richmond Samurai Pathologist Maryville TN From Donna.Willis <@t> baylorhealth.edu Fri Jul 19 12:09:57 2013 From: Donna.Willis <@t> baylorhealth.edu (Willis, Donna G.) Date: Fri Jul 19 12:10:23 2013 Subject: [Histonet] Transcriptionist Productivity Message-ID: <2572B4D63B62E64A8078D8BBE34D4078312366@BHDASVEXML2.bhcs.pvt> For Managers of Anatomic Pathology Transcriptionist, do you have productivity standards for your staff? If yes, would you please share them. Thanks, Donna Willis, HT/HTL (ASCP) Anatomic Pathology Manager Baylor University Medical Center-Dallas ph. 214-820-2465 office ph. 214-725-6184 mobile donna.willis@baylorhealth.edu ********************************************************************** This e-mail may contain confidential and/or privileged information. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. From christina.thurby <@t> bms.com Fri Jul 19 12:52:59 2013 From: christina.thurby <@t> bms.com (Thurby, Christina) Date: Fri Jul 19 12:53:03 2013 Subject: [Histonet] RE: CLIA Compliance Regulations for histology staff coverage In-Reply-To: <033f7a34-41b5-41da-b43b-d0efc38500c8@ushpwbmsmhp001.one.ads.bms.com> References: <033f7a34-41b5-41da-b43b-d0efc38500c8@ushpwbmsmhp001.one.ads.bms.com> Message-ID: Hi Akemi, Please refer to this article in Advance magazine from 2010. The second page has the CLIA regulations you are looking for. Features: The Grossing Histotechnologist in Surgical Pathology Part 1 of this 2-part article explores regulatory requirements. By Izak B. Dimenstein, MD, PhD, HT(ASCP) Posted on: May 6, 2010 http://laboratory-manager.advanceweb.com/features/article-2/the-grossing-histotechnologist-in-surgical-pathology.aspx Christina Thurby Bristol Myers Squibb 812-307-2093 > 5. Re: CLIA Compliance Regulations for histology staff coverage > (Jennifer MacDonald) >From: Akemi Allison >To: Histonet >Date: 07/18/2013 08:34 AM >Subject: [Histonet] CLIA Compliance Regulations for histology >staff >coverage >Sent by: histonet-bounces@lists.utsouthwestern.edu > > > >Good morning to all in Histoland! > >Hopefully, one of you can help me with a question which was proposed to >me >by a fellow histologist working in a very small GI histology lab. > >Here is what information she gave me: She is training a histology >assistant in house to do all the histology technical responsibilities at >the request of the GI doctors. She said her OJT assistant is doing a >great job technically, but she has not yet registered in school to >finish >her AA Degree so she can sit for the HT exam. She has been working >under >her instruction for 1 year. Her lab is California State and CLIA >licensed. > >The GI doctors want the assistant to cover for her while she is on >vacation. The pathologist who is the medical director does not think >that CLIA Regulations allows this and wants the specimens sent out >during >her absence. She needs the CLIA Regulations stating what the guidelines >are so her lab is complying to regulations. She didn???t want to call >CLIA >because it would send a RED FLAG up. I also didn???t want to call them >because they may ask me what the name of the lab was that was proposing >this question. > >I told her that this was most likely illegal, but she needs it in black >and white. Do any of you have that information or a web link to go to >at >your finger tips? She needs the regulations by next week. > >Thank you in advance for your help, >Akemi > >Akemi Allison-Tacha, BS, HT/HTL (ASCP) >Pathology Manager >Monterey Bay GI Consultants >23 Upper Ragsdale Drive, Suite 200 >Monterey, CA 93940 >(381) 375-3577 X117 >Email: aallison@montereygi.com >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. From sae2001 <@t> med.cornell.edu Fri Jul 19 16:03:03 2013 From: sae2001 <@t> med.cornell.edu (Scott A. Ely) Date: Fri Jul 19 16:03:09 2013 Subject: [Histonet] RE: Histonet Digest, Vol 116, Issue 20 In-Reply-To: <6bc2134e-47d7-473a-bcc5-2da726ed8e82@NYSGCAS02.a.wcmc-ad.net> References: <6bc2134e-47d7-473a-bcc5-2da726ed8e82@NYSGCAS02.a.wcmc-ad.net> Message-ID: We decalcify bone marrow core biopsies in EDTA with HCL, for 1 hour. This works very well for us. However, we have found that the HCL makes it impossible to do in situ hybridization with some probes (other probes work OK). Do any of you do ISH on bone marrow core biopsies? Does it work well with all needed probes, especially kappa and lambda? If so, how do you decalcify? Thank you. ------------------------------------------------------------------------------- Scott Ely, MD MPH Associate Director, Hematopathology Fellowship Program Section of Hematopathology Department of Pathology Weill Medical College of Cornell University New York Presbyterian Hospital Room: Starr 715 525 E. 68th Street New York, NY 10065 PH: 212-746-2442 FAX: 212-746-2009 http://www.cornellphysicians.com/scottely/ Legal Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the original intended recipient(s) selected by Dr. Ely and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the recipient specified by Dr. Ely, please contact the sender by reply e-mail and destroy all copies of the original message. ------------------------------------------------------------------------------- ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of histonet-request@lists.utsouthwestern.edu [histonet-request@lists.utsouthwestern.edu] Sent: Friday, July 19, 2013 1:02 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 116, Issue 20 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: histology humor on youtube (susanbachus@verizon.net) 2. Re: Carnoy solution (Bob Richmond) ---------------------------------------------------------------------- Message: 1 Date: Fri, 19 Jul 2013 12:51:58 -0400 From: Subject: Re: [Histonet] histology humor on youtube To: "Emily Sours" , Message-ID: <1C89DD2B9F0B4B2AA2B6FAE610672E47@UserPC> Content-Type: text/plain; format=flowed; charset=iso-8859-1; reply-type=original Sorry, I don't even know what a farker is! This was shared on a veterinarian's FB page! I almost sent the Lady Science one, which I think is hysterical, but I was afraid it wasn't relevant enough to histology per se (all biochemistry). But I'm glad someone else did. Well, maybe these will give a creative histologist some ideas... -----Original Message----- From: Emily Sours Sent: Friday, July 19, 2013 12:39 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] histology humor on youtube Hey, I just found that today too! Are you a farker?? I almost sent it to the list, but then decided someone had already done so and I missed it. I heard that the university is upset because it's gone viral, something about inappropriate behavior in the lab. NO HAVING FUN, EVER!! :) Emily "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." -Chuck Palahniuk, "Haunted" On Fri, Jul 19, 2013 at 10:56 AM, wrote: > I'm afraid I don't have the original query any more, but I just stumbled > onto this video from a Pathology lab, which does include some histology. > Be > forewarned that there's some profanity. https://www.youtube.com/watch?** > v=ZL7a3_JRWQY > > > ______________________________**_________________ > Histonet mailing list > Histonet@lists.utsouthwestern.**edu > http://lists.utsouthwestern.**edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Fri, 19 Jul 2013 12:56:55 -0400 From: Bob Richmond Subject: [Histonet] Re: Carnoy solution To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Carnoy's fixative contains chloroform, and that's enough reason not to use it. In addition to its toxicity, chloroform dissolves methacrylate plastic, as I found out the hard way once when I tried to fix a specimen in Carnoy's fixative in a methacrylate container because I had nothing else. I've seen Carnoy's fixative used as a "disclosing fixative" for finding mesenteric lymph nodes in colectomy specimens. There's no excuse for this. There are a number of proprietary fixatives (such as Surgipath's O-Fix) for this purpose, or if you're allowed to brew your own, I use Davidson's fixative (3 parts water, 3 parts reagent alcohol, 2 parts 37% formaldehyde [NOT 10% NBF], 1 part glacial acetic acid.) Bob Richmond Samurai Pathologist Maryville TN ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 116, Issue 20 ***************************************** From zipper210 <@t> gmail.com Sat Jul 20 21:09:29 2013 From: zipper210 <@t> gmail.com (a b) Date: Sat Jul 20 21:09:34 2013 Subject: [Histonet] Spirochete IHC on H-Pylori infected Tissue ? Message-ID: So here I am working my Saturday night, running an Immuno for Monday, only to open the fridge and find no more H-Pylori antibody cause we're out! : ( Can I substitute with Spirochete Antigen, and If so will the result will be as strong? As a standard procedure, I am doing a Warthin Starry on it also, Advice would be great. Thanks Belma Woodcraft ( Histology Lead ) From jaylundgren <@t> gmail.com Sun Jul 21 14:42:49 2013 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Sun Jul 21 14:42:55 2013 Subject: [Histonet] Re: Carnoy solution In-Reply-To: References: Message-ID: Whenever I am asked to make Carnoy's I reply, "Vroom-vroom!". Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) On Fri, Jul 19, 2013 at 11:56 AM, Bob Richmond wrote: > Carnoy's fixative contains chloroform, and that's enough reason not to use > it. In addition to its toxicity, chloroform dissolves methacrylate plastic, > as I found out the hard way once when I tried to fix a specimen in Carnoy's > fixative in a methacrylate container because I had nothing else. > > I've seen Carnoy's fixative used as a "disclosing fixative" for finding > mesenteric lymph nodes in colectomy specimens. There's no excuse for this. > There are a number of proprietary fixatives (such as Surgipath's O-Fix) for > this purpose, or if you're allowed to brew your own, I use Davidson's > fixative (3 parts water, 3 parts reagent alcohol, 2 parts 37% formaldehyde > [NOT 10% NBF], 1 part glacial acetic acid.) > > Bob Richmond > Samurai Pathologist > Maryville TN > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From rjbuesa <@t> yahoo.com Sun Jul 21 15:11:34 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sun Jul 21 15:11:38 2013 Subject: [Histonet] Spirochete IHC on H-Pylori infected Tissue ? In-Reply-To: References: Message-ID: <1374437494.60048.YahooMailNeo@web163106.mail.bf1.yahoo.com> You are overlooking the essential characteristic of IHC, namely, specificity. Ab to H-Pilory will react only with H-Pilory, and the same goes for Spirochete. You will be wasting all your reagents and, worse of everything, your supervisor will wonder about your knowledge. Ren? J. From: a b To: histonet@lists.utsouthwestern.edu Sent: Saturday, July 20, 2013 10:09 PM Subject: [Histonet] Spirochete IHC on H-Pylori infected Tissue ? So here I am working my Saturday night, running an Immuno for Monday, only to open the fridge and find no more H-Pylori antibody cause we're out! : ( Can I substitute with Spirochete Antigen, and If so will the result will be as strong? As a standard procedure, I am doing a Warthin Starry on it also, Advice would be great. Thanks Belma Woodcraft ( Histology Lead ) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cpyse <@t> x-celllab.com Mon Jul 22 07:26:12 2013 From: cpyse <@t> x-celllab.com (Cindy Pyse) Date: Mon Jul 22 07:26:16 2013 Subject: [Histonet] Transcriptionist Productivity In-Reply-To: <2572B4D63B62E64A8078D8BBE34D4078312366@BHDASVEXML2.bhcs.pvt> References: <2572B4D63B62E64A8078D8BBE34D4078312366@BHDASVEXML2.bhcs.pvt> Message-ID: <000f01ce86d6$a720a1a0$f561e4e0$@x-celllab.com> I also would be interested in any replies. Cindy Cindy Pyse CLT, HT(ASCP) Laboratory Manager X-Cell Laboratories of WNY 20 Northpointe Parkway Ste 100 Amherst, NY 14228 716-250-9235 Ext. 232 cpyse@x-celllab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Willis, Donna G. Sent: Friday, July 19, 2013 1:10 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Transcriptionist Productivity For Managers of Anatomic Pathology Transcriptionist, do you have productivity standards for your staff? If yes, would you please share them. Thanks, Donna Willis, HT/HTL (ASCP) Anatomic Pathology Manager Baylor University Medical Center-Dallas ph. 214-820-2465 office ph. 214-725-6184 mobile donna.willis@baylorhealth.edu ********************************************************************** This e-mail may contain confidential and/or privileged information. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Mon Jul 22 08:27:53 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Jul 22 08:28:00 2013 Subject: [Histonet] Transcriptionist Productivity In-Reply-To: <000f01ce86d6$a720a1a0$f561e4e0$@x-celllab.com> References: <2572B4D63B62E64A8078D8BBE34D4078312366@BHDASVEXML2.bhcs.pvt> <000f01ce86d6$a720a1a0$f561e4e0$@x-celllab.com> Message-ID: <1374499673.49766.YahooMailNeo@web163103.mail.bf1.yahoo.com> Go to http://www.histosearch.com/rene.html and you will find data on this aspect in the Staffing article. Ren? J. From: Cindy Pyse To: "'Willis, Donna G.'" ; histonet@lists.utsouthwestern.edu Sent: Monday, July 22, 2013 8:26 AM Subject: RE: [Histonet] Transcriptionist Productivity I also would be interested in any replies. Cindy Cindy Pyse CLT, HT(ASCP) Laboratory Manager X-Cell Laboratories of WNY 20 Northpointe Parkway Ste 100 Amherst, NY 14228 716-250-9235 Ext. 232 cpyse@x-celllab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Willis, Donna G. Sent: Friday, July 19, 2013 1:10 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Transcriptionist Productivity For Managers of Anatomic Pathology Transcriptionist, do you have productivity standards for your staff?? If yes, would you please share them. Thanks, Donna Willis, HT/HTL (ASCP) Anatomic Pathology Manager Baylor University Medical Center-Dallas ph. 214-820-2465 office ph. 214-725-6184 mobile donna.willis@baylorhealth.edu ********************************************************************** This e-mail may contain confidential and/or privileged information. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Valerie.Hannen <@t> parrishmed.com Mon Jul 22 10:46:56 2013 From: Valerie.Hannen <@t> parrishmed.com (Hannen, Valerie) Date: Mon Jul 22 10:47:07 2013 Subject: [Histonet] Knife sharpener glass plate Message-ID: <450B7A81EDA0C54E97C53D60F00776C3232B4BB620@isexstore03> I am going to go out on a limb here and ask what some may think is a really silly question. Does anyone have any rectangular( not the ones notched into a semi-triangle) glass plates that fit the AO 925? I also am seeking an instruction manual for it too. I was fortunate enough to be given one of these knife sharpeners by a very generous donor, but since I have never seen this model before, I am not sure how to "refrost" my plates. I received one rectangular plate and 3 of the semi- triangular ones. The sharpener does not appear to have anywhere to attach a mechanism that would hold the plates face to face so that they may be "refrosted". Any help and advice is greatly appreciated. Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.hannen@parrishmed.com =================== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" =================== From Heidi.Hawthorne <@t> onassignment.com Mon Jul 22 11:58:09 2013 From: Heidi.Hawthorne <@t> onassignment.com (Heidi Hawthorne) Date: Mon Jul 22 11:58:23 2013 Subject: [Histonet] Part-time Histo needed- East Bay of San Fran Message-ID: <519812AD3D64B24C9311DB9774CF73E2930EE87C@oasslcexm03.oaifield.onasgn.com> Part-time contract position (local contract, not a travel opportunity!) in the East Bay of San Francisco. Great way to supplement a full-time job! Must have grossing experience. Schedule would be in the afternoon/early evening hours. Other requirements-hospital lab experience a must and minimum requirement of an AA degree. For immediate consideration, contact: Kara Eula, Recruiter On Assignment, Inc. t: (510) 663-8622 Kara.Eula@onassignment.com From erikaswanson83 <@t> yahoo.com Mon Jul 22 12:10:41 2013 From: erikaswanson83 <@t> yahoo.com (Erika Swanson) Date: Mon Jul 22 12:11:01 2013 Subject: [Histonet] Unsubscribe. Message-ID: <4E627161-4588-4BB5-8CBA-6A06D0BC68A2@yahoo.com> Unsubscribe. On Jul 22, 2013, at 10:01 AM, histonet-request@lists.utsouthwestern.edu wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Re: Re: Carnoy solution (Jay Lundgren) > 2. Re: Spirochete IHC on H-Pylori infected Tissue ? (Rene J Buesa) > 3. RE: Transcriptionist Productivity (Cindy Pyse) > 4. Re: Transcriptionist Productivity (Rene J Buesa) > 5. Knife sharpener glass plate (Hannen, Valerie) > 6. Part-time Histo needed- East Bay of San Fran (Heidi Hawthorne) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Sun, 21 Jul 2013 14:42:49 -0500 > From: Jay Lundgren > Subject: Re: [Histonet] Re: Carnoy solution > To: Bob Richmond > Cc: histonet > Message-ID: > > Content-Type: text/plain; charset=ISO-8859-1 > > Whenever I am asked to make Carnoy's I reply, "Vroom-vroom!". > > Sincerely, > > Jay A. Lundgren, M.S., HTL > (ASCP) > > > On Fri, Jul 19, 2013 at 11:56 AM, Bob Richmond wrote: > >> Carnoy's fixative contains chloroform, and that's enough reason not to use >> it. In addition to its toxicity, chloroform dissolves methacrylate plastic, >> as I found out the hard way once when I tried to fix a specimen in Carnoy's >> fixative in a methacrylate container because I had nothing else. >> >> I've seen Carnoy's fixative used as a "disclosing fixative" for finding >> mesenteric lymph nodes in colectomy specimens. There's no excuse for this. >> There are a number of proprietary fixatives (such as Surgipath's O-Fix) for >> this purpose, or if you're allowed to brew your own, I use Davidson's >> fixative (3 parts water, 3 parts reagent alcohol, 2 parts 37% formaldehyde >> [NOT 10% NBF], 1 part glacial acetic acid.) >> >> Bob Richmond >> Samurai Pathologist >> Maryville TN >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Message: 2 > Date: Sun, 21 Jul 2013 13:11:34 -0700 (PDT) > From: Rene J Buesa > Subject: Re: [Histonet] Spirochete IHC on H-Pylori infected Tissue ? > To: a b , "histonet@lists.utsouthwestern.edu" > > Message-ID: > <1374437494.60048.YahooMailNeo@web163106.mail.bf1.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > You are overlooking the essential characteristic of IHC, namely, specificity. > Ab to H-Pilory will react only with H-Pilory, and the same goes for Spirochete. > You will be wasting all your reagents and, worse of everything, your supervisor will wonder about your knowledge. > Ren? J. > > From: a b > To: histonet@lists.utsouthwestern.edu > Sent: Saturday, July 20, 2013 10:09 PM > Subject: [Histonet] Spirochete IHC on H-Pylori infected Tissue ? > > > So here I am working my Saturday night, running an Immuno for Monday, only > to open the fridge and find no more H-Pylori antibody cause we're out! : ( > Can I substitute with Spirochete Antigen, and If so will the result will be > as strong? > > As a standard procedure, I am doing a Warthin Starry on it also, > > Advice would be great. Thanks > > Belma Woodcraft ( Histology Lead ) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > Message: 3 > Date: Mon, 22 Jul 2013 08:26:12 -0400 > From: "Cindy Pyse" > Subject: RE: [Histonet] Transcriptionist Productivity > To: "'Willis, Donna G.'" , > > Message-ID: <000f01ce86d6$a720a1a0$f561e4e0$@x-celllab.com> > Content-Type: text/plain; charset="us-ascii" > > I also would be interested in any replies. > Cindy > > Cindy Pyse CLT, HT(ASCP) > Laboratory Manager > X-Cell Laboratories of WNY > 20 Northpointe Parkway Ste 100 > Amherst, NY 14228 > 716-250-9235 Ext. 232 > cpyse@x-celllab.com > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Willis, > Donna G. > Sent: Friday, July 19, 2013 1:10 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Transcriptionist Productivity > > For Managers of Anatomic Pathology Transcriptionist, do you have > productivity standards for your staff? If yes, would you please share them. > > Thanks, > > Donna Willis, HT/HTL (ASCP) > Anatomic Pathology Manager > Baylor University Medical Center-Dallas > ph. 214-820-2465 office > ph. 214-725-6184 mobile > donna.willis@baylorhealth.edu > > ********************************************************************** > This e-mail may contain confidential and/or privileged information. This > information is intended only for the use of the individual(s) and > entity(ies) to whom it is addressed. If you are the intended recipient, > further disclosures are prohibited without proper authorization. If you are > not the intended recipient (or have received this e-mail in error) please > notify the sender immediately and destroy this e-mail. Any unauthorized > copying, disclosure or distribution of the material in this e-mail is > strictly forbidden and possibly a violation of federal or state law and > regulations. Baylor Health Care System, its subsidiaries, and affiliates > hereby claim all applicable privileges related to this information. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 4 > Date: Mon, 22 Jul 2013 06:27:53 -0700 (PDT) > From: Rene J Buesa > Subject: Re: [Histonet] Transcriptionist Productivity > To: Cindy Pyse , "'Willis, Donna G.'" > , "histonet@lists.utsouthwestern.edu" > > Message-ID: > <1374499673.49766.YahooMailNeo@web163103.mail.bf1.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Go to http://www.histosearch.com/rene.html and you will find data on this aspect in the Staffing article. > Ren? J. > > From: Cindy Pyse > To: "'Willis, Donna G.'" ; histonet@lists.utsouthwestern.edu > Sent: Monday, July 22, 2013 8:26 AM > Subject: RE: [Histonet] Transcriptionist Productivity > > > I also would be interested in any replies. > Cindy > > Cindy Pyse CLT, HT(ASCP) > Laboratory Manager > X-Cell Laboratories of WNY > 20 Northpointe Parkway Ste 100 > Amherst, NY 14228 > 716-250-9235 Ext. 232 > cpyse@x-celllab.com > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Willis, > Donna G. > Sent: Friday, July 19, 2013 1:10 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Transcriptionist Productivity > > For Managers of Anatomic Pathology Transcriptionist, do you have > productivity standards for your staff? If yes, would you please share them. > > Thanks, > > Donna Willis, HT/HTL (ASCP) > Anatomic Pathology Manager > Baylor University Medical Center-Dallas > ph. 214-820-2465 office > ph. 214-725-6184 mobile > donna.willis@baylorhealth.edu > > ********************************************************************** > This e-mail may contain confidential and/or privileged information. This > information is intended only for the use of the individual(s) and > entity(ies) to whom it is addressed. If you are the intended recipient, > further disclosures are prohibited without proper authorization. If you are > not the intended recipient (or have received this e-mail in error) please > notify the sender immediately and destroy this e-mail. Any unauthorized > copying, disclosure or distribution of the material in this e-mail is > strictly forbidden and possibly a violation of federal or state law and > regulations. Baylor Health Care System, its subsidiaries, and affiliates > hereby claim all applicable privileges related to this information. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > Message: 5 > Date: Mon, 22 Jul 2013 11:46:56 -0400 > From: "Hannen, Valerie" > Subject: [Histonet] Knife sharpener glass plate > To: "'histonet@lists.utsouthwestern.edu'" > > Message-ID: <450B7A81EDA0C54E97C53D60F00776C3232B4BB620@isexstore03> > Content-Type: text/plain; charset="us-ascii" > > I am going to go out on a limb here and ask what some may think is a really silly question. > > Does anyone have any rectangular( not the ones notched into a semi-triangle) glass plates that fit the AO 925? > > I also am seeking an instruction manual for it too. > > I was fortunate enough to be given one of these knife sharpeners by a very generous donor, but since I have never seen this model before, I am not sure how to "refrost" my plates. I received one rectangular plate and 3 of the semi- triangular ones. The sharpener does not appear to have anywhere to attach a mechanism that would hold the plates face to face so that they may be "refrosted". > > Any help and advice is greatly appreciated. > > > Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) > Histology Section Chief > Parrish Medical Center > 951 N. Washington Ave. > Titusville, Florida 32976 > Phone:(321) 268-6333 ext. 7506 > Fax: (321) 268-6149 > valerie.hannen@parrishmed.com > > > =================== > "This email is intended solely for the use of the individual to > whom it is addressed and may contain information that is > privileged, confidential or otherwise exempt from disclosure > under applicable law. If the reader of this email is not the > intended recipient or the employee or agent responsible for > delivering the message to the intended recipient, you are > hereby notified that any dissemination, distribution, or > copying of this communication is strictly prohibited. If you > have received this communication in error, please immediately > delete this message. Thank you" > =================== > > ------------------------------ > > Message: 6 > Date: Mon, 22 Jul 2013 16:58:09 +0000 > From: Heidi Hawthorne > Subject: [Histonet] Part-time Histo needed- East Bay of San Fran > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > <519812AD3D64B24C9311DB9774CF73E2930EE87C@oasslcexm03.oaifield.onasgn.com> > > Content-Type: text/plain; charset="us-ascii" > > Part-time contract position (local contract, not a travel opportunity!) in the East Bay of San Francisco. Great way to supplement a full-time job! Must have grossing experience. Schedule would be in the afternoon/early evening hours. Other requirements-hospital lab experience a must and minimum requirement of an AA degree. > > > > For immediate consideration, contact: > > Kara Eula, Recruiter > > On Assignment, Inc. > > t: (510) 663-8622 > > Kara.Eula@onassignment.com > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 116, Issue 23 > ***************************************** From Kimberly.Blundon <@t> drdc-rddc.gc.ca Mon Jul 22 12:17:06 2013 From: Kimberly.Blundon <@t> drdc-rddc.gc.ca (Blundon, Kimberly) Date: Mon Jul 22 12:17:11 2013 Subject: [Histonet] Unsubscribe Message-ID: Unsubscribe From trevorcwicks <@t> hotmail.com Sat Jul 20 16:39:35 2013 From: trevorcwicks <@t> hotmail.com (Trevor Wicks) Date: Mon Jul 22 19:08:16 2013 Subject: [Histonet] Fwd: Histonet Digest, Vol 116, Issue 21 Message-ID: twicks@sidra.org Sent from Samsung Mobile -------- Original message -------- Subject: Histonet Digest, Vol 116, Issue 21 From: histonet-request@lists.utsouthwestern.edu To: histonet@lists.utsouthwestern.edu CC: Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Transcriptionist Productivity (Willis, Donna G.) 2. RE: CLIA Compliance Regulations for histology staff coverage (Thurby, Christina) 3. RE: Histonet Digest, Vol 116, Issue 20 (Scott A. Ely) ---------------------------------------------------------------------- Message: 1 Date: Fri, 19 Jul 2013 17:09:57 +0000 From: "Willis, Donna G." Subject: [Histonet] Transcriptionist Productivity To: "histonet@lists.utsouthwestern.edu" Message-ID: <2572B4D63B62E64A8078D8BBE34D4078312366@BHDASVEXML2.bhcs.pvt> Content-Type: text/plain; charset="ISO-8859-1" For Managers of Anatomic Pathology Transcriptionist, do you have productivity standards for your staff? If yes, would you please share them. Thanks, Donna Willis, HT/HTL (ASCP) Anatomic Pathology Manager Baylor University Medical Center-Dallas ph. 214-820-2465 office ph. 214-725-6184 mobile donna.willis@baylorhealth.edu ********************************************************************** This e-mail may contain confidential and/or privileged information. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. ------------------------------ Message: 2 Date: Fri, 19 Jul 2013 13:52:59 -0400 From: "Thurby, Christina" Subject: [Histonet] RE: CLIA Compliance Regulations for histology staff coverage To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Hi Akemi, Please refer to this article in Advance magazine from 2010. The second page has the CLIA regulations you are looking for. Features: The Grossing Histotechnologist in Surgical Pathology Part 1 of this 2-part article explores regulatory requirements. By Izak B. Dimenstein, MD, PhD, HT(ASCP) Posted on: May 6, 2010 http://laboratory-manager.advanceweb.com/features/article-2/the-grossing-histotechnologist-in-surgical-pathology.aspx Christina Thurby Bristol Myers Squibb 812-307-2093 > 5. Re: CLIA Compliance Regulations for histology staff coverage > (Jennifer MacDonald) >From: Akemi Allison >To: Histonet >Date: 07/18/2013 08:34 AM >Subject: [Histonet] CLIA Compliance Regulations for histology >staff >coverage >Sent by: histonet-bounces@lists.utsouthwestern.edu > > > >Good morning to all in Histoland! > >Hopefully, one of you can help me with a question which was proposed to >me >by a fellow histologist working in a very small GI histology lab. > >Here is what information she gave me: She is training a histology >assistant in house to do all the histology technical responsibilities at >the request of the GI doctors. She said her OJT assistant is doing a >great job technically, but she has not yet registered in school to >finish >her AA Degree so she can sit for the HT exam. She has been working >under >her instruction for 1 year. Her lab is California State and CLIA >licensed. > >The GI doctors want the assistant to cover for her while she is on >vacation. The pathologist who is the medical director does not think >that CLIA Regulations allows this and wants the specimens sent out >during >her absence. She needs the CLIA Regulations stating what the guidelines >are so her lab is complying to regulations. She didn???t want to call >CLIA >because it would send a RED FLAG up. I also didn???t want to call them >because they may ask me what the name of the lab was that was proposing >this question. > >I told her that this was most likely illegal, but she needs it in black >and white. Do any of you have that information or a web link to go to >at >your finger tips? She needs the regulations by next week. > >Thank you in advance for your help, >Akemi > >Akemi Allison-Tacha, BS, HT/HTL (ASCP) >Pathology Manager >Monterey Bay GI Consultants >23 Upper Ragsdale Drive, Suite 200 >Monterey, CA 93940 >(381) 375-3577 X117 >Email: aallison@montereygi.com >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. ------------------------------ Message: 3 Date: Fri, 19 Jul 2013 21:03:03 +0000 From: "Scott A. Ely" Subject: [Histonet] RE: Histonet Digest, Vol 116, Issue 20 To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" We decalcify bone marrow core biopsies in EDTA with HCL, for 1 hour. This works very well for us. However, we have found that the HCL makes it impossible to do in situ hybridization with some probes (other probes work OK). Do any of you do ISH on bone marrow core biopsies? Does it work well with all needed probes, especially kappa and lambda? If so, how do you decalcify? Thank you. ------------------------------------------------------------------------------- Scott Ely, MD MPH Associate Director, Hematopathology Fellowship Program Section of Hematopathology Department of Pathology Weill Medical College of Cornell University New York Presbyterian Hospital Room: Starr 715 525 E. 68th Street New York, NY 10065 PH: 212-746-2442 FAX: 212-746-2009 http://www.cornellphysicians.com/scottely/ Legal Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the original intended recipient(s) selected by Dr. Ely and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the recipient specified by Dr. Ely, please contact the sender by reply e-mail and destroy all copies of the original message. ------------------------------------------------------------------------------- ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of histonet-request@lists.utsouthwestern.edu [histonet-request@lists.utsouthwestern.edu] Sent: Friday, July 19, 2013 1:02 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 116, Issue 20 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: histology humor on youtube (susanbachus@verizon.net) 2. Re: Carnoy solution (Bob Richmond) ---------------------------------------------------------------------- Message: 1 Date: Fri, 19 Jul 2013 12:51:58 -0400 From: Subject: Re: [Histonet] histology humor on youtube To: "Emily Sours" , Message-ID: <1C89DD2B9F0B4B2AA2B6FAE610672E47@UserPC> Content-Type: text/plain; format=flowed; charset=iso-8859-1; reply-type=original Sorry, I don't even know what a farker is! This was shared on a veterinarian's FB page! I almost sent the Lady Science one, which I think is hysterical, but I was afraid it wasn't relevant enough to histology per se (all biochemistry). But I'm glad someone else did. Well, maybe these will give a creative histologist some ideas... -----Original Message----- From: Emily Sours Sent: Friday, July 19, 2013 12:39 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] histology humor on youtube Hey, I just found that today too! Are you a farker?? I almost sent it to the list, but then decided someone had already done so and I missed it. I heard that the university is upset because it's gone viral, something about inappropriate behavior in the lab. NO HAVING FUN, EVER!! :) Emily "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." -Chuck Palahniuk, "Haunted" On Fri, Jul 19, 2013 at 10:56 AM, wrote: > I'm afraid I don't have the original query any more, but I just stumbled > onto this video from a Pathology lab, which does include some histology. > Be > forewarned that there's some profanity. https://www.youtube.com/watch?** > v=ZL7a3_JRWQY > > > ______________________________**_________________ > Histonet mailing list > Histonet@lists.utsouthwestern.**edu > http://lists.utsouthwestern.**edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Fri, 19 Jul 2013 12:56:55 -0400 From: Bob Richmond Subject: [Histonet] Re: Carnoy solution To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Carnoy's fixative contains chloroform, and that's enough reason not to use it. In addition to its toxicity, chloroform dissolves methacrylate plastic, as I found out the hard way once when I tried to fix a specimen in Carnoy's fixative in a methacrylate container because I had nothing else. I've seen Carnoy's fixative used as a "disclosing fixative" for finding mesenteric lymph nodes in colectomy specimens. There's no excuse for this. There are a number of proprietary fixatives (such as Surgipath's O-Fix) for this purpose, or if you're allowed to brew your own, I use Davidson's fixative (3 parts water, 3 parts reagent alcohol, 2 parts 37% formaldehyde [NOT 10% NBF], 1 part glacial acetic acid.) Bob Richmond Samurai Pathologist Maryville TN ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 116, Issue 20 ***************************************** ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 116, Issue 21 ***************************************** From mmooreht <@t> yahoo.com Tue Jul 23 11:35:46 2013 From: mmooreht <@t> yahoo.com (Michelle Moore) Date: Tue Jul 23 11:35:50 2013 Subject: [Histonet] Formalin Message-ID: <1374597346.60782.YahooMailNeo@web125102.mail.ne1.yahoo.com> Good day Histoland! ? Can anyone that is using a formalin substitute that is happy with the quality?of fixation please share! ? Thank you. Michelle Moore SRMC St. Thomas USVI From sbaldwin <@t> mhhcc.org Tue Jul 23 13:06:32 2013 From: sbaldwin <@t> mhhcc.org (Sara Baldwin/mhhcc.org) Date: Tue Jul 23 13:07:44 2013 Subject: [Histonet] High complexity tests Message-ID: Histonetters we are going to be going thru a state inspection and was getting some homework done, is Nexus special stainer (ventana) hig complexity tests? What about frozen sections? Any help will be appreciated! Thanks Histology/Cytology Supervisor S. Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph 812-996-0210, 0216, Fax 812-996-0232, Pager 812-481-0897, Cell 812-887-3357 From jo-ann.bader <@t> mcgill.ca Tue Jul 23 13:31:32 2013 From: jo-ann.bader <@t> mcgill.ca (Jo-Ann Bader, Ms.) Date: Tue Jul 23 13:31:36 2013 Subject: [Histonet] Micro Tissue Arrayer Message-ID: <8C36045F0065CE48906E684F15FD4CB6320E31DB@EXMBX2010-6.campus.MCGILL.CA> We are currently in the market for a Micro Tissue Arrayer. I would like to hear from other labs that have one, which one do you have and is it easy to getr service for them. Thanks Jo-Ann Bader Histology Co-Ordinator Goodman Cancer Centre McGill University 1160 Pine Ave. W - Rm 312 Montreal, QC, Canada H3G 1Y6 Tel: 514-398-8270 From gmartin <@t> marshallmedical.org Tue Jul 23 13:46:30 2013 From: gmartin <@t> marshallmedical.org (Martin, Gary) Date: Tue Jul 23 13:46:38 2013 Subject: [Histonet] Sections Message-ID: <6ED9D4252F278841A0593D3D788AF24C1C72713A@mailsvr.MARSHMED.local> One of our pathologist has requested a change in the way we cut GI specimens. We presently put three levels on one slide. Exp. we face the block, cut a ribbon and take one section of that ribbon and catch on the slide. We then progress into the block and repeat the process until we have three levels on the slide. The new suggestion is to simply face the block, create a ribbon, and take three to four section of that ribbon and that's it no more progression into the block. The thinking is that the Pathologist will see what they need to in those levels, and if not they will call for "deepers". I have never cut like this and just wanted to hear some thoughts on this method. Thanks Gary From jorourke <@t> allied360.com Tue Jul 23 13:47:02 2013 From: jorourke <@t> allied360.com (Judy O'Rourke) Date: Tue Jul 23 13:47:08 2013 Subject: [Histonet] Study Highlights Risk of Errors in Processing Biopsy Tissues in Anatomic Pathology Labs In-Reply-To: Message-ID: Histonetters... A study in the Journal of Histotechnology highlights the all-too-real risk of errors that can be made in the processing of biopsy tissue in anatomic pathology labs. Read the story here: http://www.clpmag.com/all-news/24255-risk-of-errors-in-processing-biopsy-tissues-in-anatomic-pathology-labs Send me your questions for the study authors. I can post them on histonet and/or anonymously, on our website-your choice. Thank you, Judy JUDY O'ROURKE | Chief Editor Clinical Lab Products Allied Media 7101 College Blvd, Suite 400 Overland Park, KS 66210 office 913.894.6923 x687 | fax 913.894.6932 Direct office 619.659.1065 | Direct fax 619.659.1065 jorourke@allied360.com | www.clpmag.com From jorourke <@t> allied360.com Tue Jul 23 13:56:16 2013 From: jorourke <@t> allied360.com (Judy O'Rourke) Date: Tue Jul 23 13:56:36 2013 Subject: [Histonet] Study Highlights Risk of Errors in Processing Biopsy Tissues in Anatomic Pathology Labs In-Reply-To: Message-ID: I can post the questions/answers on histonet. On 7/23/13 11:47 AM, "Judy O'Rourke" wrote: >Histonetters... > >A study in the Journal of Histotechnology highlights the all-too-real >risk of errors that can be made in the processing of biopsy tissue in >anatomic pathology labs. > >Read the story here: >http://www.clpmag.com/all-news/24255-risk-of-errors-in-processing-biopsy-t >issues-in-anatomic-pathology-labs > >Send me your questions for the study authors. I can post them on histonet >and/or anonymously, on our website-your choice. > >Thank you, > >Judy > >JUDY O'ROURKE | Chief Editor > >Clinical Lab Products >Allied Media >7101 College Blvd, Suite 400 Overland Park, KS 66210 >office 913.894.6923 x687 | fax 913.894.6932 >Direct office 619.659.1065 | Direct fax 619.659.1065 >jorourke@allied360.com | www.clpmag.com > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tony.auge <@t> gmail.com Tue Jul 23 14:04:34 2013 From: tony.auge <@t> gmail.com (Tony Auge) Date: Tue Jul 23 14:04:39 2013 Subject: [Histonet] Sections In-Reply-To: <6ED9D4252F278841A0593D3D788AF24C1C72713A@mailsvr.MARSHMED.local> References: <6ED9D4252F278841A0593D3D788AF24C1C72713A@mailsvr.MARSHMED.local> Message-ID: We cut the way your Pathologist is requesting at my lab, it's simpler and faster then the way you are currently cutting. I was used to cutting the way you described but this way is much easier. Check with your other Pathologists that they will be ok with the switch. I have certain Pathologist that will order more deepers because they want to see more of the block. It will speed up cutting in the first place but may take more time to cut deepers later in the day. -- Tony Auge HTL (ASCP) QIHC Cell: (651) 373-4768 Email: tony.auge@gmail.com From Joyce.Weems <@t> emoryhealthcare.org Tue Jul 23 14:21:37 2013 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Tue Jul 23 14:23:13 2013 Subject: [Histonet] RE: Sections In-Reply-To: <6ED9D4252F278841A0593D3D788AF24C1C72713A@mailsvr.MARSHMED.local> References: <6ED9D4252F278841A0593D3D788AF24C1C72713A@mailsvr.MARSHMED.local> Message-ID: If placing more than 1 section of the same level on a slide, I've always cut three slides. I've never had a pathologist who only wanted to see one level. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martin, Gary Sent: Tuesday, July 23, 2013 2:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sections One of our pathologist has requested a change in the way we cut GI specimens. We presently put three levels on one slide. Exp. we face the block, cut a ribbon and take one section of that ribbon and catch on the slide. We then progress into the block and repeat the process until we have three levels on the slide. The new suggestion is to simply face the block, create a ribbon, and take three to four section of that ribbon and that's it no more progression into the block. The thinking is that the Pathologist will see what they need to in those levels, and if not they will call for "deepers". I have never cut like this and just wanted to hear some thoughts on this method. Thanks Gary _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From flnails <@t> texaschildrens.org Tue Jul 23 14:46:52 2013 From: flnails <@t> texaschildrens.org (Nails, Felton) Date: Tue Jul 23 14:46:56 2013 Subject: [Histonet] RE: Sections In-Reply-To: References: <6ED9D4252F278841A0593D3D788AF24C1C72713A@mailsvr.MARSHMED.local> Message-ID: <327E034F1892504289B7A17EC71DF9F302254E@TCFMSG03.ad.texaschildrenshospital.org> I have heard of both methods, currently we put double ribbons on one slide. The only time we have repeats is if the initial cut is superficial. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Tuesday, July 23, 2013 2:22 PM To: 'Martin, Gary'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Sections If placing more than 1 section of the same level on a slide, I've always cut three slides. I've never had a pathologist who only wanted to see one level. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martin, Gary Sent: Tuesday, July 23, 2013 2:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sections One of our pathologist has requested a change in the way we cut GI specimens. We presently put three levels on one slide. Exp. we face the block, cut a ribbon and take one section of that ribbon and catch on the slide. We then progress into the block and repeat the process until we have three levels on the slide. The new suggestion is to simply face the block, create a ribbon, and take three to four section of that ribbon and that's it no more progression into the block. The thinking is that the Pathologist will see what they need to in those levels, and if not they will call for "deepers". I have never cut like this and just wanted to hear some thoughts on this method. Thanks Gary _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ______________________________________________________________________ From Dingersoll <@t> aplaboratories.com Tue Jul 23 14:49:17 2013 From: Dingersoll <@t> aplaboratories.com (Dingersoll@aplaboratories.com) Date: Tue Jul 23 14:49:22 2013 Subject: [Histonet] Study Highlights Risk of Errors in Processing Biopsy Tissues in Anatomic Pathology Labs Message-ID: <20130723124917.073ecbdb5144cf8a05e574ee22bfb11a.a4252137a6.wbe@email17.secureserver.net> Donna S. Ingersoll, B.S., HTL, CT(ASCP) -------- Original Message ----- Subject: [Histonet] Study Highlights Risk of Errors in Processing Biopsy Tissues in Anatomic Pathology Labs From: Judy O'Rourke Date: Tue, July 23, 2013 2:47 pm To: "[2]histonet@lists.utsouthwestern.edu" Histonetters... A study i all-too-real risk of errors biopsy tissue in anatomic pathology Read the story here: [4]http://www.clpmag.com/all-news/24255-risk-of-errors-in-processing -biopsy-tissues-in-anatomic-pathology-labs Send me your qu histonet and/or anonymous Thank you, Judy JUDY O'ROURKE | Chief Editor Clinical Lab Product Allied Media 7101 College Blvd, Suite 400 Overland Park, KS 6 office 913.894.6923 x687 | fax 913.894.6932 Direct offic [5]jorourke@allied360.com<[6]mailto:jorourke@allied360.com> | [7]www.clpmag.com __________________________ Histonet mailing list [8]Histonet@lists.utsouthwestern.edu References 1. 3D"mailto:jorourke@allied360.com" 2. 3D"mailto:histo 3. 3D"mailto:histonet@lists.utsouthwestern.edu" 4. 3D"http://www.clpmag.com/al 5. 3D"mailto:jorourke@a 6. 3D"mailto:jorourke@alli 7. 3D"http://www.cl=/ 8. 3D"mailto:H 9. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" From jaylundgren <@t> gmail.com Tue Jul 23 14:56:15 2013 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Tue Jul 23 14:56:19 2013 Subject: [Histonet] Sections In-Reply-To: References: <6ED9D4252F278841A0593D3D788AF24C1C72713A@mailsvr.MARSHMED.local> Message-ID: I've worked places where they've used all kinds of leveling methods, specifying number of microns between levels, etc. I cut exactly whatever the pathologist wants me to cut. I've traveled for 16 years now. Think about some of the wacky crap I've seen. A GOOD histotech, in my humble opinion, ( assuming a new, state of the art mirotome, new disposable blade, cold, rough cut, hydrated blocks) should be able to lay out a 21 section 3um ribbon of a GI bx longways on the water bath in one go. Said histotech would always ignore the first and last sections of said 21 section ribbon (one is touching the waterbath, and the terminal one touched the forceps/stick/finger/etc) and pick up the 2nd/3rd, 12th/13th, and 19th/20th sections. This is 3 levels. A BAD histotech will lay out a six section ribbon, take six sections (and a floater), and call it 3 levels. Make absolutely sure that you are rough cutting into the block adequately to provide a full face section. Most of your recuts will come from under-faced blocks. But try to cut and float nice LOOOOOOONG ribbons to pick up your sections from and you will get nicer sections and less recuts. What takes time cutting (traditional) levels is the refacing and recooling/rehydration of the block. If you can get true levels off of one long ribbon (not 6 adjacent sections), and not increase recuts as a result, you win twice. So does the patient. :) Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) On Tue, Jul 23, 2013 at 2:04 PM, Tony Auge wrote: > We cut the way your Pathologist is requesting at my lab, it's simpler and > faster then the way you are currently cutting. I was used to cutting the > way you described but this way is much easier. Check with your other > Pathologists that they will be ok with the switch. I have certain > Pathologist that will order more deepers because they want to see more of > the block. It will speed up cutting in the first place but may take more > time to cut deepers later in the day. > -- > > Tony Auge HTL (ASCP) QIHC > Cell: (651) 373-4768 > Email: tony.auge@gmail.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jaylundgren <@t> gmail.com Tue Jul 23 15:21:18 2013 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Tue Jul 23 15:21:23 2013 Subject: [Histonet] Study Highlights Risk of Errors in Processing Biopsy Tissues in Anatomic Pathology Labs In-Reply-To: <20130723124917.073ecbdb5144cf8a05e574ee22bfb11a.a4252137a6.wbe@email17.secureserver.net> References: <20130723124917.073ecbdb5144cf8a05e574ee22bfb11a.a4252137a6.wbe@email17.secureserver.net> Message-ID: I have a question. How does this corporate sponsored, softball, self published, meta-science, piece of marketing get to masquerade as a scientific paper? Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) On Tue, Jul 23, 2013 at 2:49 PM, wrote: > > Donna S. Ingersoll, B.S., HTL, CT(ASCP) > > > > > > > > -------- Original Message ----- --- > > Subject: [Histonet] Study Highlights Risk of Errors in Processing Biopsy > > Tissues in Anatomic Pathology Labs > > From: Judy O'Rourke <[1]jorourke@allied360.com> ; > > Date: Tue, July 23, 2013 2:47 pm > > To: "[2]histonet@lists.utsouthwestern.edu" > > <[3]histonet@lists.uts outhwestern.edu> > > > > Histonetters... > > > > A study i n the Journal of Histotechnology highlights the > all-too-real risk of errors that can be made in the processing of > biopsy tissue in anatomic pathology labs. > > > > Read the story here: > [4]http://www.clpmag.com/all-news/24255-risk-of-errors-in-processing > -biopsy-tissues-in-anatomic-pathology-labs > > > > Send me your qu estions for the study authors. I can post them on > histonet and/or anonymous ly, on our website-your choice. > > > > Thank you, > > > > Judy > > > JUDY O'ROURKE | Chief Editor > > > > Clinical Lab Product s > > Allied Media > > 7101 College Blvd, Suite 400 Overland Park, KS 6 6210 > > office 913.894.6923 x687 | fax 913.894.6932 > > Direct offic e 619.659.1065 | Direct fax 619.659.1065 > > [5]jorourke@allied360.com<[6]mailto:jorourke@allied360.com> | > [7]www.clpmag.com > > > > > > __________________________ _____________________ > > Histonet mailing list > > [8]Histonet@lists.utsouthwestern.edu > > [9]ht tp://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > References > > 1. 3D"mailto:jorourke@allied360.com" > 2. 3D"mailto:histo 3. 3D"mailto:histonet@lists.utsouthwestern.edu" > 4. 3D"http://www.clpmag.com/al 5. 3D"mailto:jorourke@a 6. > 3D"mailto:jorourke@alli 7. 3D"http://www.cl/ > 8. 3D"mailto:H 9. 3D" > http://lists.utsouthwestern.edu/mailman/listinfo/histonet" > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jshelley <@t> sanfordburnham.org Tue Jul 23 15:36:55 2013 From: jshelley <@t> sanfordburnham.org (John Shelley) Date: Tue Jul 23 15:36:59 2013 Subject: [Histonet] Dok-4 Message-ID: Hi All, Wondering if anyone has used this antibody on human FFPE tissue and what the retrieval method was. I am going to use a breast tumor as my optimizing control but have limited supply. I am using Santa Cruz antibody C-16: sc-130133. Your help will be greatly appreciated!!!! Kind Regards! John J Shelley Research Specialist, Histology Core Facility Sanford-Burnham Medical Research Institute at Lake Nona From jorourke <@t> allied360.com Tue Jul 23 15:51:55 2013 From: jorourke <@t> allied360.com (Judy O'Rourke) Date: Tue Jul 23 15:52:28 2013 Subject: [Histonet] Study Highlights Risk of Errors in Processing Biopsy Tissues in Anatomic Pathology Labs In-Reply-To: Message-ID: My apologies if I've posted something that is not useful/helpful. I posted it because it was included in the Journal of Histotechnology, and was hoping to be a conduit for actionable information. Judy From: Jay Lundgren > Date: Tuesday, July 23, 2013 1:21 PM To: "Dingersoll@aplaboratories.com" > Cc: Judy O'Rourke >, "histonet@lists.utsouthwestern.edu" > Subject: Re: [Histonet] Study Highlights Risk of Errors in Processing Biopsy Tissues in Anatomic Pathology Labs I have a question. How does this corporate sponsored, softball, self published, meta-science, piece of marketing get to masquerade as a scientific paper? Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) On Tue, Jul 23, 2013 at 2:49 PM, > wrote: Donna S. Ingersoll, B.S., HTL, CT(ASCP) -------- Original Message ----- --- Subject: [Histonet] Study Highlights Risk of Errors in Processing Biopsy Tissues in Anatomic Pathology Labs From: Judy O'Rourke <[1]jorourke@allied360.com> ; Date: Tue, July 23, 2013 2:47 pm To: "[2]histonet@lists.utsouthwestern.edu" <[3]histonet@lists.uts outhwestern.edu> Histonetters... A study i n the Journal of Histotechnology highlights the all-too-real risk of errors that can be made in the processing of biopsy tissue in anatomic pathology labs. Read the story here: [4]http://www.clpmag.com/all-news/24255-risk-of-errors-in-processing -biopsy-tissues-in-anatomic-pathology-labs Send me your qu estions for the study authors. I can post them on histonet and/or anonymous ly, on our website-your choice. Thank you, Judy JUDY O'ROURKE | Chief Editor Clinical Lab Product s Allied Media 7101 College Blvd, Suite 400 Overland Park, KS 6 6210 office 913.894.6923 x687 | fax 913.894.6932 Direct offic e 619.659.1065 | Direct fax 619.659.1065 [5]jorourke@allied360.com<[6]mailto:jorourke@allied360.com> | [7]www.clpmag.com __________________________ _____________________ Histonet mailing list [8]Histonet@lists.utsouthwestern.edu [9]ht tp://lists.utsouthwestern.edu/mailman/listinfo/histonet References 1. 3D"mailto:jorourke@allied360.com" 2. 3D"mailto:histo 3. 3D"mailto:histonet@lists.utsouthwestern.edu" 4. 3D"http://www.clpmag.com/al 5. 3D"mailto:jorourke@a 6. 3D"mailto:jorourke@alli 7. 3D"http://www.cl/ 8. 3D"mailto:H 9. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jaylundgren <@t> gmail.com Tue Jul 23 17:49:49 2013 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Tue Jul 23 17:49:55 2013 Subject: [Histonet] Study Highlights Risk of Errors in Processing Biopsy Tissues in Anatomic Pathology Labs In-Reply-To: References: Message-ID: Sorry, I was a little snarky. Ventana holds key patents on the reaction pad that allow the staining to be done on individual, flat slides, in an automated stainer. So they sponsor a "paper" to prove the hypothesis that everyone not using their equipment is doing it wrong. And they pick a silver stain for the "experiment" in which they know most people re-use their silver solution. Then they publish it in a publication in which they are major advertisers. This is marketing disguising itself as science. On Tue, Jul 23, 2013 at 3:51 PM, Judy O'Rourke wrote: > My apologies if I've posted something that is not useful/helpful. > > I posted it because it was included in the *Journal of Histotechnology,* and > was hoping to be a conduit for actionable information. > > Judy > > From: Jay Lundgren > Date: Tuesday, July 23, 2013 1:21 PM > To: "Dingersoll@aplaboratories.com" > Cc: Judy O'Rourke , " > histonet@lists.utsouthwestern.edu" > Subject: Re: [Histonet] Study Highlights Risk of Errors in Processing > Biopsy Tissues in Anatomic Pathology Labs > > I have a question. How does this corporate sponsored, softball, self > published, meta-science, piece of marketing get to masquerade as a > scientific paper? > > Sincerely, > > Jay A. Lundgren, > M.S., HTL (ASCP) > > > On Tue, Jul 23, 2013 at 2:49 PM, wrote: > >> >> Donna S. Ingersoll, B.S., HTL, CT(ASCP) >> >> >> >> >> >> >> >> -------- Original Message ----- --- >> >> Subject: [Histonet] Study Highlights Risk of Errors in Processing >> Biopsy >> >> Tissues in Anatomic Pathology Labs >> >> From: Judy O'Rourke <[1]jorourke@allied360.com> ; >> >> Date: Tue, July 23, 2013 2:47 pm >> >> To: "[2]histonet@lists.utsouthwestern.edu" >> >> <[3]histonet@lists.uts outhwestern.edu> >> >> >> >> Histonetters... >> >> >> >> A study i n the Journal of Histotechnology highlights the >> all-too-real risk of errors that can be made in the processing of >> biopsy tissue in anatomic pathology labs. >> >> >> >> Read the story here: >> [4]http://www.clpmag.com/all-news/24255-risk-of-errors-in-processing -biopsy-tissues-in-anatomic-pathology-labs >> >> >> >> Send me your qu estions for the study authors. I can post them on >> histonet and/or anonymous ly, on our website-your choice. >> >> >> >> Thank you, >> >> >> >> Judy >> >> >> JUDY O'ROURKE | Chief Editor >> >> >> >> Clinical Lab Product s >> >> Allied Media >> >> 7101 College Blvd, Suite 400 Overland Park, KS 6 6210 >> >> office 913.894.6923 x687 | fax 913.894.6932 >> >> Direct offic e 619.659.1065 | Direct fax 619.659.1065 >> >> [5]jorourke@allied360.com<[6]mailto:jorourke@allied360.com> | >> [7]www.clpmag.com >> >> >> >> >> >> __________________________ _____________________ >> >> Histonet mailing list >> >> [8]Histonet@lists.utsouthwestern.edu >> >> [9]ht tp://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> >> >> >> >> References >> >> 1. 3D"mailto:jorourke@allied360.com" >> 2. 3D"mailto:histo 3. 3D"mailto:histonet@lists.utsouthwestern.edu" >> 4. 3D"http://www.clpmag.com/al 5. 3D"mailto:jorourke@a 6. >> 3D"mailto:jorourke@alli 7. 3D"http://www.cl/ >> 8. 3D"mailto:H 9. 3D" >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet" >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > From Tony_Reilly <@t> health.qld.gov.au Tue Jul 23 18:09:01 2013 From: Tony_Reilly <@t> health.qld.gov.au (Tony Reilly) Date: Tue Jul 23 18:09:31 2013 Subject: [Histonet] Sections In-Reply-To: <6ED9D4252F278841A0593D3D788AF24C1C72713A@mailsvr.MARSHMED.local> References: <6ED9D4252F278841A0593D3D788AF24C1C72713A@mailsvr.MARSHMED.local> Message-ID: <51EF99AD.411C.0039.0@health.qld.gov.au> Hi Martin You should cut as your pathologist requests as it is their name on the final report. However if I were you I would keep a record of how many cases in the future you need to go back to cut extra levels. If you are going back to a majority of cases present the data to your pathologist as evidence that it would be more efficient to cut levels in the first instance. If in the majority of cases you are not cutting levels then you have saved yourself a lot of work. Having said that there are some instances in GI biopsies where the pathology can be very focal and levels are good insurance. regards Tony Tony Reilly B.App.Sc. , M.Sc. Chief Scientist, Anatomical Pathology Pathology Queensland-PA Laboratory ________________________________________________ Health Services Support Agency | Department of Health Level 1, Building 15,Princess Alexandra Hospital Ipswich Road,WOOLLOONGABBA Qld4102 Ph: 07 3176 2412 Mob: 0402 139411 Fax: 07 3176 2930 Email: tony_reilly@health.qld.gov.au Web: www.health.qld.gov.au/qhcss/ >>> "Martin, Gary" 7/24/2013 4:46 am >>> One of our pathologist has requested a change in the way we cut GI specimens. We presently put three levels on one slide. Exp. we face the block, cut a ribbon and take one section of that ribbon and catch on the slide. We then progress into the block and repeat the process until we have three levels on the slide. The new suggestion is to simply face the block, create a ribbon, and take three to four section of that ribbon and that's it no more progression into the block. The thinking is that the Pathologist will see what they need to in those levels, and if not they will call for "deepers". I have never cut like this and just wanted to hear some thoughts on this method. Thanks Gary _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. 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Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ********************************************************************************** From joelleweaver <@t> hotmail.com Tue Jul 23 18:14:10 2013 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Tue Jul 23 18:14:17 2013 Subject: [Histonet] RE: Sections In-Reply-To: <327E034F1892504289B7A17EC71DF9F302254E@TCFMSG03.ad.texaschildrenshospital.org> References: <6ED9D4252F278841A0593D3D788AF24C1C72713A@mailsvr.MARSHMED.local>, , <327E034F1892504289B7A17EC71DF9F302254E@TCFMSG03.ad.texaschildrenshospital.org> Message-ID: I have heard of and performed both methods. The variables seem to be with a ribbon, that if some fold, wrinkle, or fall off, you basically have more sections of the same depth on one slide. That being said, if that level is bad or superficial, you will get recuts/deepers. If they are only needing one depth into the tissue for initial diagnosis or are worried about conserving tissue, then a ribbon might work. If they want progression, then you need more levels, one slide or many. Without going full rotations there wouldn't be much progression in depth in a single ribbon, especially at the thickness one would normally cut biopsy sections. Personally I like levels, one somewhat superficial section that is still a complete outline, one slightly deeper and one fully into the specimen without cutting anything away. Personally levels not only save recuts to me, but provide only one slide for labeling ( less labeling error opportunities), higher staining throughput/TAT/cases, less storage, less slides...you get the idea. of course ultimately it is up to the Pathologist and sometimes others, but was there some reason given for the change in preference? Joelle Weaver MAOM, HTL (ASCP) QIHC > From: flnails@texaschildrens.org > To: Joyce.Weems@emoryhealthcare.org; gmartin@marshallmedical.org; histonet@lists.utsouthwestern.edu > Date: Tue, 23 Jul 2013 19:46:52 +0000 > CC: > Subject: [Histonet] RE: Sections > > I have heard of both methods, currently we put double ribbons on one slide. > The only time we have repeats is if the initial cut is superficial. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. > Sent: Tuesday, July 23, 2013 2:22 PM > To: 'Martin, Gary'; histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: Sections > > If placing more than 1 section of the same level on a slide, I've always cut three slides. I've never had a pathologist who only wanted to see one level. > > Joyce Weems > Pathology Manager > 678-843-7376 Phone > 678-843-7831 Fax > joyce.weems@emoryhealthcare.org > > > > www.saintjosephsatlanta.org > 5665 Peachtree Dunwoody Road > Atlanta, GA 30342 > > This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martin, Gary > Sent: Tuesday, July 23, 2013 2:47 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Sections > > One of our pathologist has requested a change in the way we cut GI specimens. We presently put three levels on one slide. Exp. we face the block, cut a ribbon and take one section of that ribbon and catch on the slide. We then progress into the block and repeat the process until we have three levels on the slide. The new suggestion is to simply face the block, create a ribbon, and take three to four section of that ribbon and that's it no more progression into the block. The thinking is that the Pathologist will see what they need to in those levels, and if not they will call for "deepers". I have never cut like this and just wanted to hear some thoughts on this method. > > Thanks > > Gary > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ________________________________ > > This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. > > If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ______________________________________________________________________ > CONFIDENTIALITY NOTICE: > The information in this e-mail may be confidential and/or > privileged. If you are not the intended recipient or an > authorized representative of the intended recipient, you > are hereby notified that any review, dissemination, or > copying of this e-mail and its attachments, if any, or > the information contained herein is prohibited. If you > have received this e-mail in error, please immediately > notify the sender by return e-mail and delete this e-mail > from your computer system. Thank you. > ______________________________________________________________________ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Tue Jul 23 18:15:54 2013 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Tue Jul 23 18:15:59 2013 Subject: [Histonet] Sections In-Reply-To: References: <6ED9D4252F278841A0593D3D788AF24C1C72713A@mailsvr.MARSHMED.local>, , Message-ID: Agreed. Joelle Weaver MAOM, HTL (ASCP) QIHC > Date: Tue, 23 Jul 2013 14:56:15 -0500 > From: jaylundgren@gmail.com > To: tony.auge@gmail.com > Subject: Re: [Histonet] Sections > CC: histonet@lists.utsouthwestern.edu; gmartin@marshallmedical.org > > I've worked places where they've used all kinds of leveling methods, > specifying number of microns between levels, etc. I cut exactly whatever > the pathologist wants me to cut. I've traveled for 16 years now. Think > about some of the wacky crap I've seen. > A GOOD histotech, in my humble opinion, ( assuming a new, state of the > art mirotome, new disposable blade, cold, rough cut, hydrated blocks) > should be able to lay out a 21 section 3um ribbon of a GI bx longways on > the water bath in one go. > Said histotech would always ignore the first and last sections of > said 21 section ribbon (one is touching the waterbath, and the terminal one > touched the forceps/stick/finger/etc) and pick up the 2nd/3rd, 12th/13th, > and 19th/20th sections. This is 3 levels. > A BAD histotech will lay out a six section ribbon, take six sections > (and a floater), and call it 3 levels. > Make absolutely sure that you are rough cutting into the block > adequately to provide a full face section. Most of your recuts will come > from under-faced blocks. But try to cut and float nice LOOOOOOONG ribbons > to pick up your sections from and you will get nicer sections and less > recuts. What takes time cutting (traditional) levels is the refacing and > recooling/rehydration of the block. > If you can get true levels off of one long ribbon (not 6 adjacent > sections), and not increase recuts as a result, you win twice. So does the > patient. :) > > Sincerely, > > Jay A. Lundgren, M.S., HTL (ASCP) > > > > > > > > > > > On Tue, Jul 23, 2013 at 2:04 PM, Tony Auge wrote: > > > We cut the way your Pathologist is requesting at my lab, it's simpler and > > faster then the way you are currently cutting. I was used to cutting the > > way you described but this way is much easier. Check with your other > > Pathologists that they will be ok with the switch. I have certain > > Pathologist that will order more deepers because they want to see more of > > the block. It will speed up cutting in the first place but may take more > > time to cut deepers later in the day. > > -- > > > > Tony Auge HTL (ASCP) QIHC > > Cell: (651) 373-4768 > > Email: tony.auge@gmail.com > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Tue Jul 23 18:24:39 2013 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Tue Jul 23 18:24:44 2013 Subject: [Histonet] Study Highlights Risk of Errors in Processing Biopsy Tissues in Anatomic Pathology Labs In-Reply-To: References: , Message-ID: This reminds me of an earlier article on this subject ( that did not appear to have a corporate sponsor), and it compared several variables and points that introduce slide contamination, (stain bath cross-contamination, water bath and some others). It had some pretty good comparisons I thought at the time I read it. It looks to me like maybe this article was the jumping off point for this information? Even though there is a marketing angle perhaps, either way, it is good to reinforce those practice-methods and QC. If I can relocate that article I can post it if there is interest in this topic. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: jorourke@allied360.com > To: jaylundgren@gmail.com; Dingersoll@aplaboratories.com > Date: Tue, 23 Jul 2013 20:51:55 +0000 > Subject: Re: [Histonet] Study Highlights Risk of Errors in Processing Biopsy Tissues in Anatomic Pathology Labs > CC: histonet@lists.utsouthwestern.edu > > My apologies if I've posted something that is not useful/helpful. > > I posted it because it was included in the Journal of Histotechnology, and was hoping to be a conduit for actionable information. > > Judy > > From: Jay Lundgren > > Date: Tuesday, July 23, 2013 1:21 PM > To: "Dingersoll@aplaboratories.com" > > Cc: Judy O'Rourke >, "histonet@lists.utsouthwestern.edu" > > Subject: Re: [Histonet] Study Highlights Risk of Errors in Processing Biopsy Tissues in Anatomic Pathology Labs > > I have a question. How does this corporate sponsored, softball, self published, meta-science, piece of marketing get to masquerade as a scientific paper? > > Sincerely, > > Jay A. Lundgren, M.S., HTL (ASCP) > > > On Tue, Jul 23, 2013 at 2:49 PM, > wrote: > > Donna S. Ingersoll, B.S., HTL, CT(ASCP) > > > > > > > > -------- Original Message ----- --- > > Subject: [Histonet] Study Highlights Risk of Errors in Processing Biopsy > > Tissues in Anatomic Pathology Labs > > From: Judy O'Rourke <[1]jorourke@allied360.com> ; > > Date: Tue, July 23, 2013 2:47 pm > > To: "[2]histonet@lists.utsouthwestern.edu" > > <[3]histonet@lists.uts outhwestern.edu> > > > > Histonetters... > > > > A study i n the Journal of Histotechnology highlights the > all-too-real risk of errors that can be made in the processing of > biopsy tissue in anatomic pathology labs. > > > > Read the story here: > [4]http://www.clpmag.com/all-news/24255-risk-of-errors-in-processing -biopsy-tissues-in-anatomic-pathology-labs > > > > Send me your qu estions for the study authors. I can post them on > histonet and/or anonymous ly, on our website-your choice. > > > > Thank you, > > > > Judy > > > JUDY O'ROURKE | Chief Editor > > > > Clinical Lab Product s > > Allied Media > > 7101 College Blvd, Suite 400 Overland Park, KS 6 6210 > > office 913.894.6923 x687 | fax 913.894.6932 > > Direct offic e 619.659.1065 | Direct fax 619.659.1065 > > [5]jorourke@allied360.com<[6]mailto:jorourke@allied360.com> | > [7]www.clpmag.com > > > > > > __________________________ _____________________ > > Histonet mailing list > > [8]Histonet@lists.utsouthwestern.edu > > [9]ht tp://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > References > > 1. 3D"mailto:jorourke@allied360.com" > 2. 3D"mailto:histo 3. 3D"mailto:histonet@lists.utsouthwestern.edu" > 4. 3D"http://www.clpmag.com/al 5. 3D"mailto:jorourke@a 6. 3D"mailto:jorourke@alli 7. 3D"http://www.cl/ > 8. 3D"mailto:H 9. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Tue Jul 23 19:15:50 2013 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Tue Jul 23 19:15:56 2013 Subject: [Histonet] High complexity tests In-Reply-To: References: Message-ID: Sara This link has CLIA list-hopes it helps http://www.cms.gov/Regulations-and-Guidance/Legislation/CLIA/Categorization_of_Tests.html Joelle Weaver MAOM, HTL (ASCP) QIHC > From: sbaldwin@mhhcc.org > To: histonet@lists.utsouthwestern.edu > Date: Tue, 23 Jul 2013 14:06:32 -0400 > Subject: [Histonet] High complexity tests > > Histonetters > we are going to be going thru a state inspection and was getting some homework done, is Nexus special stainer (ventana) hig complexity tests? > What about frozen sections? Any help will be appreciated! > > Thanks > Histology/Cytology Supervisor > S. Kathy Baldwin, SCT (ASCP) > Memorial Hospital and Health Care Center > sbaldwin@mhhcc.org > Ph 812-996-0210, 0216, Fax 812-996-0232, > Pager 812-481-0897, Cell 812-887-3357 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tony.henwood <@t> health.nsw.gov.au Tue Jul 23 19:27:04 2013 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Tue Jul 23 19:27:38 2013 Subject: [Histonet] Study Highlights Risk of Errors in Processing Biopsy Tissues in Anatomic Pathology Labs In-Reply-To: References: , Message-ID: <6D6BD1DE8A5571489398B392A38A71579D28DC76@xmdb04.nch.kids> Joelle, Could you possibly be referring to: Platt E, Sommer P; McDonald L, Bennett A, Hunt J (2009) "Tissue Floaters and Contaminants in the Histology Laboratory" Arch Pathol Lab Med 133:973-978 Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelle weaver Sent: Wednesday, 24 July 2013 9:25 AM To: Judy O'Rourke; Jay Lundgren; Dingersoll@aplaboratories.com Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Study Highlights Risk of Errors in Processing Biopsy Tissues in Anatomic Pathology Labs This reminds me of an earlier article on this subject ( that did not appear to have a corporate sponsor), and it compared several variables and points that introduce slide contamination, (stain bath cross-contamination, water bath and some others). It had some pretty good comparisons I thought at the time I read it. It looks to me like maybe this article was the jumping off point for this information? Even though there is a marketing angle perhaps, either way, it is good to reinforce those practice-methods and QC. If I can relocate that article I can post it if there is interest in this topic. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: jorourke@allied360.com > To: jaylundgren@gmail.com; Dingersoll@aplaboratories.com > Date: Tue, 23 Jul 2013 20:51:55 +0000 > Subject: Re: [Histonet] Study Highlights Risk of Errors in Processing Biopsy Tissues in Anatomic Pathology Labs > CC: histonet@lists.utsouthwestern.edu > > My apologies if I've posted something that is not useful/helpful. > > I posted it because it was included in the Journal of Histotechnology, and was hoping to be a conduit for actionable information. > > Judy > > From: Jay Lundgren > > > Date: Tuesday, July 23, 2013 1:21 PM > To: > "Dingersoll@aplaboratories.com" > > > Cc: Judy O'Rourke > >, > "histonet@lists.utsouthwestern.edu n.edu>" > n.edu>> > Subject: Re: [Histonet] Study Highlights Risk of Errors in Processing > Biopsy Tissues in Anatomic Pathology Labs > > I have a question. How does this corporate sponsored, softball, self published, meta-science, piece of marketing get to masquerade as a scientific paper? > > Sincerely, > > Jay A. > Lundgren, M.S., HTL (ASCP) > > > On Tue, Jul 23, 2013 at 2:49 PM, > wrote: > > Donna S. Ingersoll, B.S., HTL, CT(ASCP) > > > > > > > > -------- Original Message ----- --- > > Subject: [Histonet] Study Highlights Risk of Errors in Processing Biopsy > > Tissues in Anatomic Pathology Labs > > From: Judy O'Rourke > <[1]jorourke@allied360.com> ; > > Date: Tue, July 23, 2013 2:47 pm > > To: "[2]histonet@lists.utsouthwestern.edu" > > <[3]histonet@lists.uts > outhwestern.edu> > > > > Histonetters... > > > > A study i n the Journal of Histotechnology highlights the > all-too-real risk of errors that can be made in the processing of > biopsy tissue in anatomic pathology labs. > > > > Read the story here: > [4]http://www.clpmag.com/all-news/24255-risk-of-errors-in-processing -biopsy-tissues-in-anatomic-pathology-labs > > > > Send me your qu estions for the study authors. I can post them on > histonet and/or anonymous ly, on our website-your choice. > > > > Thank you, > > > > Judy > > > JUDY O'ROURKE | Chief Editor > > > > Clinical Lab Product s > > Allied Media > > 7101 College Blvd, Suite 400 Overland Park, KS 6 6210 > > office 913.894.6923 x687 | fax > 913.894.6932 > > Direct offic e 619.659.1065 | Direct fax > 619.659.1065 > > [5]jorourke@allied360.com<[6]mailto:jorourke@allied360.com> | > [7]www.clpmag.com > > > > > > __________________________ _____________________ > > Histonet mailing list > > [8]Histonet@lists.utsouthwestern.edu ern.edu> > > [9]ht > tp://lists.utsouthwestern.edu/mailman/listinfo/histonet tsouthwestern.edu/mailman/listinfo/histonet> > > > > > > > > References > > 1. 3D"mailto:jorourke@allied360.com" > 2. 3D"mailto:histo 3. 3D"mailto:histonet@lists.utsouthwestern.edu" > 4. 3D"http://www.clpmag.com/al 5. 3D"mailto:jorourke@a 6. 3D"mailto:jorourke@alli 7. 3D"http://www.cl/ > 8. 3D"mailto:H 9. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu .edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From zipper210 <@t> gmail.com Wed Jul 24 04:55:10 2013 From: zipper210 <@t> gmail.com (a b) Date: Wed Jul 24 04:55:15 2013 Subject: [Histonet] Spirochete IHC on H-Pylori infected Tissue ? In-Reply-To: <1374437494.60048.YahooMailNeo@web163106.mail.bf1.yahoo.com> References: <1374437494.60048.YahooMailNeo@web163106.mail.bf1.yahoo.com> Message-ID: Rene that sounded really rude, its a simple yes or no question. Some medical journals list H-Pylori as a "Spirochete"! that's why I asked in the first place... I wonder what your supervisor thinks of your knowledge, especially when you spelled H-Pylori > " H-pilori" ??? hmm. -__- On Sun, Jul 21, 2013 at 3:11 PM, Rene J Buesa wrote: > You are overlooking the essential characteristic of IHC, namely, > specificity. > Ab to H-Pilory will react only with H-Pilory, and the same goes for > Spirochete. > You will be wasting all your reagents and, worse of everything, your > supervisor will wonder about your knowledge. > Ren? J. > > *From:* a b > *To:* histonet@lists.utsouthwestern.edu > *Sent:* Saturday, July 20, 2013 10:09 PM > *Subject:* [Histonet] Spirochete IHC on H-Pylori infected Tissue ? > > So here I am working my Saturday night, running an Immuno for Monday, only > to open the fridge and find no more H-Pylori antibody cause we're out! : ( > Can I substitute with Spirochete Antigen, and If so will the result will be > as strong? > > As a standard procedure, I am doing a Warthin Starry on it also, > > Advice would be great. Thanks > > Belma Woodcraft ( Histology Lead ) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From joelleweaver <@t> hotmail.com Wed Jul 24 07:11:44 2013 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Wed Jul 24 07:11:50 2013 Subject: [Histonet] Study Highlights Risk of Errors in Processing Biopsy Tissues in Anatomic Pathology Labs In-Reply-To: <6D6BD1DE8A5571489398B392A38A71579D28DC76@xmdb04.nch.kids> References: , , , , <6D6BD1DE8A5571489398B392A38A71579D28DC76@xmdb04.nch.kids> Message-ID: Yes, that is the article! Thank you Tony for the citation. I have a hard copy in a file somewhere..... Joelle Weaver MAOM, HTL (ASCP) QIHC > From: tony.henwood@health.nsw.gov.au > To: joelleweaver@hotmail.com; jorourke@allied360.com; jaylundgren@gmail.com; dingersoll@aplaboratories.com > CC: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Study Highlights Risk of Errors in Processing Biopsy Tissues in Anatomic Pathology Labs > Date: Wed, 24 Jul 2013 00:27:04 +0000 > > Joelle, > > Could you possibly be referring to: > > Platt E, Sommer P; McDonald L, Bennett A, Hunt J (2009) "Tissue Floaters and Contaminants in the Histology Laboratory" Arch Pathol Lab Med 133:973-978 > > Regards > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) > Laboratory Manager & Senior Scientist > Tel: 612 9845 3306 > Fax: 612 9845 3318 > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead > Locked Bag 4001, Westmead NSW 2145, AUSTRALIA > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelle weaver > Sent: Wednesday, 24 July 2013 9:25 AM > To: Judy O'Rourke; Jay Lundgren; Dingersoll@aplaboratories.com > Cc: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Study Highlights Risk of Errors in Processing Biopsy Tissues in Anatomic Pathology Labs > > This reminds me of an earlier article on this subject ( that did not appear to have a corporate sponsor), and it compared several variables and points that introduce slide contamination, (stain bath cross-contamination, water bath and some others). It had some pretty good comparisons I thought at the time I read it. It looks to me like maybe this article was the jumping off point for this information? Even though there is a marketing angle perhaps, either way, it is good to reinforce those practice-methods and QC. If I can relocate that article I can post it if there is interest in this topic. > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > From: jorourke@allied360.com > > To: jaylundgren@gmail.com; Dingersoll@aplaboratories.com > > Date: Tue, 23 Jul 2013 20:51:55 +0000 > > Subject: Re: [Histonet] Study Highlights Risk of Errors in Processing Biopsy Tissues in Anatomic Pathology Labs > > CC: histonet@lists.utsouthwestern.edu > > > > My apologies if I've posted something that is not useful/helpful. > > > > I posted it because it was included in the Journal of Histotechnology, and was hoping to be a conduit for actionable information. > > > > Judy > > > > From: Jay Lundgren > > > > > Date: Tuesday, July 23, 2013 1:21 PM > > To: > > "Dingersoll@aplaboratories.com" > > > > > Cc: Judy O'Rourke > > >, > > "histonet@lists.utsouthwestern.edu > n.edu>" > > > n.edu>> > > Subject: Re: [Histonet] Study Highlights Risk of Errors in Processing > > Biopsy Tissues in Anatomic Pathology Labs > > > > I have a question. How does this corporate sponsored, softball, self published, meta-science, piece of marketing get to masquerade as a scientific paper? > > > > Sincerely, > > > > Jay A. > > Lundgren, M.S., HTL (ASCP) > > > > > > On Tue, Jul 23, 2013 at 2:49 PM, > wrote: > > > > Donna S. Ingersoll, B.S., HTL, CT(ASCP) > > > > > > > > > > > > > > > > -------- Original Message ----- --- > > > > Subject: [Histonet] Study Highlights Risk of Errors in Processing Biopsy > > > > Tissues in Anatomic Pathology Labs > > > > From: Judy O'Rourke > > <[1]jorourke@allied360.com> ; > > > > Date: Tue, July 23, 2013 2:47 pm > > > > To: "[2]histonet@lists.utsouthwestern.edu" > > > > <[3]histonet@lists.uts > > outhwestern.edu> > > > > > > > > Histonetters... > > > > > > > > A study i n the Journal of Histotechnology highlights the > > all-too-real risk of errors that can be made in the processing of > > biopsy tissue in anatomic pathology labs. > > > > > > > > Read the story here: > > [4]http://www.clpmag.com/all-news/24255-risk-of-errors-in-processing -biopsy-tissues-in-anatomic-pathology-labs > > > > > > > > Send me your qu estions for the study authors. I can post them on > > histonet and/or anonymous ly, on our website-your choice. > > > > > > > > Thank you, > > > > > > > > Judy > > > > > > JUDY O'ROURKE | Chief Editor > > > > > > > > Clinical Lab Product s > > > > Allied Media > > > > 7101 College Blvd, Suite 400 Overland Park, KS 6 6210 > > > > office 913.894.6923 x687 | fax > > 913.894.6932 > > > > Direct offic e 619.659.1065 | Direct fax > > 619.659.1065 > > > > [5]jorourke@allied360.com<[6]mailto:jorourke@allied360.com> | > > [7]www.clpmag.com > > > > > > > > > > > > __________________________ _____________________ > > > > Histonet mailing list > > > > [8]Histonet@lists.utsouthwestern.edu > ern.edu> > > > > [9]ht > > tp://lists.utsouthwestern.edu/mailman/listinfo/histonet > tsouthwestern.edu/mailman/listinfo/histonet> > > > > > > > > > > > > > > > > References > > > > 1. 3D"mailto:jorourke@allied360.com" > > 2. 3D"mailto:histo 3. 3D"mailto:histonet@lists.utsouthwestern.edu" > > 4. 3D"http://www.clpmag.com/al 5. 3D"mailto:jorourke@a 6. 3D"mailto:jorourke@alli 7. 3D"http://www.cl/ > > 8. 3D"mailto:H 9. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > .edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ********************************************************************************* > This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. > > Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. > > This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. > ********************************************************************************* From DKBoyd <@t> chs.net Wed Jul 24 07:46:58 2013 From: DKBoyd <@t> chs.net (Boyd, Debbie M) Date: Wed Jul 24 07:47:09 2013 Subject: [Histonet] Sections In-Reply-To: References: <6ED9D4252F278841A0593D3D788AF24C1C72713A@mailsvr.MARSHMED.local>, , Message-ID: <7EAFE982E328304DA6CE2B677BB76246794EC457@TN001WEXMBX12.US.chs.net> It all depends on your definition of "levels" . Levels is going deeper into the block between each pick up. Serial sections are just cutting a ribbon and taking that ribbon. I don't feel you are deeper in the block when you take "sections" at 12 and 13. Sections 19 and 20 may be deep enough to be considered "level 2". You are only advancing 3um with each section. It doesn't do the patient any good to leave the tissue undiagnosed in the block. There have been times when the cancer didn't show up until level 3. I treat every biopsy as my own. Of course, you must defer to the pathologist . Our job is not to make it easier on us, ours is to serve the patient. Just my 2 cents. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelle weaver Sent: Tuesday, July 23, 2013 7:16 PM To: Jay Lundgren; Tony Auge Cc: histonet@lists.utsouthwestern.edu; Martin, Gary Subject: RE: [Histonet] Sections Agreed. Joelle Weaver MAOM, HTL (ASCP) QIHC > Date: Tue, 23 Jul 2013 14:56:15 -0500 > From: jaylundgren@gmail.com > To: tony.auge@gmail.com > Subject: Re: [Histonet] Sections > CC: histonet@lists.utsouthwestern.edu; gmartin@marshallmedical.org > > I've worked places where they've used all kinds of leveling > methods, specifying number of microns between levels, etc. I cut > exactly whatever the pathologist wants me to cut. I've traveled for > 16 years now. Think about some of the wacky crap I've seen. > A GOOD histotech, in my humble opinion, ( assuming a new, state of > the art mirotome, new disposable blade, cold, rough cut, hydrated > blocks) should be able to lay out a 21 section 3um ribbon of a GI bx > longways on the water bath in one go. > Said histotech would always ignore the first and last sections > of said 21 section ribbon (one is touching the waterbath, and the > terminal one touched the forceps/stick/finger/etc) and pick up the > 2nd/3rd, 12th/13th, and 19th/20th sections. This is 3 levels. > A BAD histotech will lay out a six section ribbon, take six > sections (and a floater), and call it 3 levels. > Make absolutely sure that you are rough cutting into the block > adequately to provide a full face section. Most of your recuts will come > from under-faced blocks. But try to cut and float nice LOOOOOOONG ribbons > to pick up your sections from and you will get nicer sections and less > recuts. What takes time cutting (traditional) levels is the refacing > and recooling/rehydration of the block. > If you can get true levels off of one long ribbon (not 6 adjacent > sections), and not increase recuts as a result, you win twice. So does > the patient. :) > > Sincerely, > > Jay A. Lundgren, M.S., HTL > (ASCP) > > > > > > > > > > > On Tue, Jul 23, 2013 at 2:04 PM, Tony Auge wrote: > > > We cut the way your Pathologist is requesting at my lab, it's > > simpler and faster then the way you are currently cutting. I was > > used to cutting the way you described but this way is much easier. > > Check with your other Pathologists that they will be ok with the > > switch. I have certain Pathologist that will order more deepers > > because they want to see more of the block. It will speed up cutting > > in the first place but may take more time to cut deepers later in the day. > > -- > > > > Tony Auge HTL (ASCP) QIHC > > Cell: (651) 373-4768 > > Email: tony.auge@gmail.com > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From TGoins <@t> mt.gov Wed Jul 24 09:25:48 2013 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Wed Jul 24 09:25:58 2013 Subject: [Histonet] Spirochete IHC on H-Pylori infected Tissue ? In-Reply-To: References: <1374437494.60048.YahooMailNeo@web163106.mail.bf1.yahoo.com> Message-ID: Belma - Whether or not the spirochete antibody would work with H. pylori depends entirely on the anti-spirochete antibody specificity. We use an anti-chlamydia antibody that hits all Chlamydia sp.; others are species specific. The data sheet or the WEB site for the spirochete antibody should provide that information. Tresa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of a b Sent: Wednesday, July 24, 2013 3:55 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Spirochete IHC on H-Pylori infected Tissue ? Rene that sounded really rude, its a simple yes or no question. Some medical journals list H-Pylori as a "Spirochete"! that's why I asked in the first place... I wonder what your supervisor thinks of your knowledge, especially when you spelled H-Pylori > " H-pilori" ??? hmm. -__- On Sun, Jul 21, 2013 at 3:11 PM, Rene J Buesa > wrote: > You are overlooking the essential characteristic of IHC, namely, > specificity. > Ab to H-Pilory will react only with H-Pilory, and the same goes for > Spirochete. > You will be wasting all your reagents and, worse of everything, your > supervisor will wonder about your knowledge. > Ren? J. > > *From:* a b > > *To:* histonet@lists.utsouthwestern.edu > *Sent:* Saturday, July 20, 2013 10:09 PM > *Subject:* [Histonet] Spirochete IHC on H-Pylori infected Tissue ? > > So here I am working my Saturday night, running an Immuno for Monday, > only to open the fridge and find no more H-Pylori antibody cause we're > out! : ( Can I substitute with Spirochete Antigen, and If so will the > result will be as strong? > > As a standard procedure, I am doing a Warthin Starry on it also, > > Advice would be great. Thanks > > Belma Woodcraft ( Histology Lead ) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rheyna <@t> lumc.edu Wed Jul 24 10:05:29 2013 From: rheyna <@t> lumc.edu (Roger Heyna) Date: Wed Jul 24 10:06:00 2013 Subject: [Histonet] Embedding Contamination Message-ID: <51EFA6E9020000230007E5DB@gwgwia1.luhs.org> Hi Histonetters: When a pathologist identifies extraneous tissue on a slide, and histology determines that the tissue contamination originates from embedding, what is your procedure for correcting the embedding? Do you remove the extraneous (or contamination) tissue from the block, recut the slide, and discard the original contaminated slide? Do you leave the extraneous tissue in the block and make a note in the report and/or on the slide? Do you keep track of these in a log somewhere? Thanks in advance for your help. Roger Maywood, IL From rjbuesa <@t> yahoo.com Wed Jul 24 11:54:53 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jul 24 11:55:02 2013 Subject: [Histonet] Embedding Contamination In-Reply-To: <51EFA6E9020000230007E5DB@gwgwia1.luhs.org> References: <51EFA6E9020000230007E5DB@gwgwia1.luhs.org> Message-ID: <1374684893.88879.YahooMailNeo@web163101.mail.bf1.yahoo.com> This how I always handled this issue: 1- document the contamination in your QC file trying to identify the tissue source; 2- train the embedding histotechs in the proper way of cleaning the embedding instruments and the forceps wells and document it also in the corresponding QC file; 3- save the slide marking the contaminating tissue fragment; 4- remove the contaminant and recut the block. Do not melt the block down, just remove the contaminant with a needle. 5- the slide to be filed with the case is the recut (step 4). Probably other people handle this issue differently, but that is how I handled it Ren? J. From: Roger Heyna To: histonet@lists.utsouthwestern.edu Sent: Wednesday, July 24, 2013 11:05 AM Subject: [Histonet] Embedding Contamination Hi Histonetters: When a pathologist identifies extraneous tissue on a slide, and histology determines that the tissue contamination originates from embedding, what is your procedure for correcting the embedding? Do you remove the extraneous (or contamination) tissue from the block, recut the slide, and discard the original contaminated slide? Do you leave the extraneous tissue in the block and make a note in the report and/or on the slide? Do you keep track of these in a log somewhere? Thanks in advance for your help. Roger Maywood, IL _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gmartin <@t> marshallmedical.org Wed Jul 24 12:44:32 2013 From: gmartin <@t> marshallmedical.org (Martin, Gary) Date: Wed Jul 24 12:44:42 2013 Subject: [Histonet] Sections Message-ID: <6ED9D4252F278841A0593D3D788AF24C1C7275DB@mailsvr.MARSHMED.local> I want to thank everyone for the great responses I received to my question about different methods of sectioning specimens. What I heard as a common response was to give the Pathologist what they need to do their job, which is exactly what I had intended and will do. Many thanks Gary From azdudley <@t> hotmail.com Wed Jul 24 14:49:39 2013 From: azdudley <@t> hotmail.com (anita dudley) Date: Wed Jul 24 14:49:42 2013 Subject: [Histonet] CAP 23045 Message-ID: Can someone please tell me are you adding this to your instrument procedure or should there be a seperate procedure for each instrument? Thanks so much, Anita Dudley From jaylundgren <@t> gmail.com Wed Jul 24 17:44:50 2013 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Wed Jul 24 17:44:58 2013 Subject: [Histonet] Embedding Contamination In-Reply-To: <1374684893.88879.YahooMailNeo@web163101.mail.bf1.yahoo.com> References: <51EFA6E9020000230007E5DB@gwgwia1.luhs.org> <1374684893.88879.YahooMailNeo@web163101.mail.bf1.yahoo.com> Message-ID: Everyone jumps on the embedder in these situations, but the contamination might be coming from the gross board or the tissue processor as well. So be sure to train the appropriate docs/PA's/histotechs how to avoid cross contamination when grossing/processing as well. And stop jumping on the embedder, 'cause the dirt from your shoes might be what's causing the contamination in the first place. Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) On Wed, Jul 24, 2013 at 11:54 AM, Rene J Buesa wrote: > This how I always handled this issue: > 1- document the contamination in your QC file trying to identify the > tissue source; > 2- train the embedding histotechs in the proper way of cleaning the > embedding instruments and the forceps wells and document it also in the > corresponding QC file; > 3- save the slide marking the contaminating tissue fragment; > 4- remove the contaminant and recut the block. Do not melt the block down, > just remove the contaminant with a needle. > 5- the slide to be filed with the case is the recut (step 4). > Probably other people handle this issue differently, but that is how I > handled it > Ren? J. > > From: Roger Heyna > To: histonet@lists.utsouthwestern.edu > Sent: Wednesday, July 24, 2013 11:05 AM > Subject: [Histonet] Embedding Contamination > > > Hi Histonetters: > > When a pathologist identifies extraneous tissue on a slide, and histology > determines that the tissue contamination originates from embedding, what is > your procedure for correcting the embedding? Do you remove the extraneous > (or contamination) tissue from the block, recut the slide, and discard the > original contaminated slide? Do you leave the extraneous tissue in the > block and make a note in the report and/or on the slide? Do you keep track > of these in a log somewhere? > > Thanks in advance for your help. > Roger > Maywood, IL > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jbro22 <@t> lsuhsc.edu Thu Jul 25 09:30:35 2013 From: jbro22 <@t> lsuhsc.edu (Browning, Jeffrey A.) Date: Thu Jul 25 09:30:44 2013 Subject: [Histonet] Histotech vacancies for routine, renal, and/or neuromuscular (Shreveport, LA) Message-ID: <2E3D5719BC42374E982EF0AD611F23629280FD79@SH-ExchMB1.master.lsuhsc.edu> Good morning, Our Pathology Department will soon be advertising vacancies for histotechnologists, with opportunity to specialize in renal specimens (routine FFPE, special stains, cryotomy, IF) or neuromuscular specimens (routine FFPE, special stains, cryotomy, enzyme histochemistry) depending on experience. If interested please contact me directly. Thank you, Jeff Browning, HTL(ASCP) Technical Director, Anatomic Pathology Department of Pathology LSU Health Science Center - Shreveport 1501 Kings Highway Shreveport, LA 71103 (318) 675-5872 jbro22@lsuhsc.edu From jshelley <@t> sanfordburnham.org Thu Jul 25 09:44:53 2013 From: jshelley <@t> sanfordburnham.org (John Shelley) Date: Thu Jul 25 09:44:58 2013 Subject: [Histonet] Dok-4 Second Request Message-ID: Hi All, I am hoping someone in research may have used this antibody in the past and may be able to shed some light on whether they were able to get this to work or not. I am running some more slides using 4 different method of retrieval to see if any of those work. Wondering if anyone has used this antibody on human FFPE tissue and what the retrieval method was. I am going to use a breast tumor as my optimizing control but have limited supply. I am using Santa Cruz antibody C-16: sc-130133. Your help will be greatly appreciated!!!! Kind Regards! John J Shelley Research Specialist, Histology Core Facility Sanford-Burnham Medical Research Institute at Lake Nona From brett_connolly <@t> merck.com Thu Jul 25 12:08:26 2013 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Thu Jul 25 12:08:36 2013 Subject: [Histonet] Leica Bond protocol question Message-ID: Hi All, I am not a Bond user, but have an antibody were a user had success using "standard protocol F" Can some please enlighten me with the specifics of that protocol? Many thanks, Brett Brett M. Connolly, Ph.D. Principal Scientist, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From sforeman <@t> labpath.com Thu Jul 25 13:39:51 2013 From: sforeman <@t> labpath.com (Susan Foreman) Date: Thu Jul 25 13:43:41 2013 Subject: [Histonet] BRAF V600 D & V600 K Message-ID: <001901ce8966$595e12d0$0c1a3870$@com> Hello, Histoland, I would appreciate any help you can offer on these antibodies. Does anyone have experience with the BRAF V600 D and V600 K? Are you running in colorectal specimen or in melanocytic (skin) tumors? Many Thanks, Susan Foreman, HT (ASCP) KDL Pathology 315 Erin Drive Knoxville, TN 37919 (865)584-1933 From katherine.bohrer <@t> merck.com Thu Jul 25 14:28:40 2013 From: katherine.bohrer <@t> merck.com (Bohrer, Katherine) Date: Thu Jul 25 14:28:54 2013 Subject: [Histonet] Coverslipper recommendations/ feedback Message-ID: <6F559D7F2112B0428CD7C49DBC7555BCC1ED86A558@USCTMXP51009.merck.com> Greetings! We are currently looking to purchase a new automated coverslipper, and looking for any experience-based feedback or recommendations to aid us in our search. Dako and Leica models are of particular interest. Thanks! Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From BDeBrosse-Serra <@t> isisph.com Thu Jul 25 14:37:46 2013 From: BDeBrosse-Serra <@t> isisph.com (Bea DeBrosse-Serra) Date: Thu Jul 25 14:37:51 2013 Subject: [Histonet] RE: Coverslipper recommendations/ feedback In-Reply-To: <6F559D7F2112B0428CD7C49DBC7555BCC1ED86A558@USCTMXP51009.merck.com> References: <6F559D7F2112B0428CD7C49DBC7555BCC1ED86A558@USCTMXP51009.merck.com> Message-ID: <493CAA64F203E14E8823737B9EE0E25F094BEAF1B2@EXCHMB01.isis.local> We like our Leica coverslipper. I guess it also depends on what kind of automated stainer you have, if you want to have continuous motion. Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bohrer, Katherine Sent: Thursday, July 25, 2013 12:29 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Coverslipper recommendations/ feedback Greetings! We are currently looking to purchase a new automated coverslipper, and looking for any experience-based feedback or recommendations to aid us in our search. Dako and Leica models are of particular interest. Thanks! Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MSHERWOOD <@t> PARTNERS.ORG Thu Jul 25 14:45:08 2013 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret) Date: Thu Jul 25 14:45:14 2013 Subject: [Histonet] RE: Coverslipper recommendations/ feedback In-Reply-To: <6F559D7F2112B0428CD7C49DBC7555BCC1ED86A558@USCTMXP51009.merck.com> References: <6F559D7F2112B0428CD7C49DBC7555BCC1ED86A558@USCTMXP51009.merck.com> Message-ID: <16F356143B1CE2459BC129BF68AD0F0F14E529E6@PHSX10MB25.partners.org> We have the Leica CV5030 coverslipper which we use independently from the stainer-Leica XLautostainer (for space issues). We love it! I might add, we purchased both as refurbished equipment about 3-4 years ago and they are still going strong--saved a lot of money by going this route. Peggy Peggy Sherwood Research Specialist, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bohrer, Katherine Sent: Thursday, July 25, 2013 3:29 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Coverslipper recommendations/ feedback Greetings! We are currently looking to purchase a new automated coverslipper, and looking for any experience-based feedback or recommendations to aid us in our search. Dako and Leica models are of particular interest. Thanks! Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From Joyce.Weems <@t> emoryhealthcare.org Thu Jul 25 14:53:23 2013 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Thu Jul 25 14:53:31 2013 Subject: [Histonet] RE: Coverslipper recommendations/ feedback In-Reply-To: <6F559D7F2112B0428CD7C49DBC7555BCC1ED86A558@USCTMXP51009.merck.com> References: <6F559D7F2112B0428CD7C49DBC7555BCC1ED86A558@USCTMXP51009.merck.com> Message-ID: We have the Leica Multistainer/Coverslipper combo - absolutely wonderful!! Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bohrer, Katherine Sent: Thursday, July 25, 2013 3:29 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Coverslipper recommendations/ feedback Greetings! We are currently looking to purchase a new automated coverslipper, and looking for any experience-based feedback or recommendations to aid us in our search. Dako and Leica models are of particular interest. Thanks! Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From Beth.Fye <@t> HCAhealthcare.com Thu Jul 25 15:29:07 2013 From: Beth.Fye <@t> HCAhealthcare.com (Beth.Fye@HCAhealthcare.com) Date: Thu Jul 25 15:29:13 2013 Subject: [Histonet] Transcriptionist Productivity (Willis, Donna G.) Message-ID: <938F8EC5A524D34EB5796E23E52781D361C40369A4@NADCWPMSGCMS05.hca.corpad.net> We have two types of transcriptionists working for our Market Pathology departments. There are onsite transcriptionists that transcribe and have many other duties such as doing send outs and answering phones. Because they have other miscellaneous duties, coming up with a standard was difficult, but we did do it. We compare their characters typed by hour worked. I add up all the character totals and then divide it between the number of transcriptionists. This is the average. Each transcriptionist must be doing at least 80% of the average. This helps to make sure that one transcriptionist is not doing less than others. We also have transcriptionists that do transcription from home. Since they do not have the interruptions or other duties, they have stricter guidelines. The minimum that they are supposed to complete is 160 characters per hour worked however they often do much more. I hope this helps, it's not an easy thing to keep track of, I know. Let me know if you need any more information. Beth A. Fye, CT (ASCP) Pathology Technical Manager HCA Richmond Hospital Laboratories office: (804) 228-6564 fax: (877)699-3895 From mikeykkk_3 <@t> live.com Thu Jul 25 20:08:40 2013 From: mikeykkk_3 <@t> live.com (Mike) Date: Thu Jul 25 20:08:52 2013 Subject: [Histonet] Tissue Message-ID: Where can I buy fresh tissue samples to test on my processor? Thanks From tony.henwood <@t> health.nsw.gov.au Thu Jul 25 21:25:01 2013 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Thu Jul 25 21:25:22 2013 Subject: [Histonet] Tissue In-Reply-To: References: Message-ID: <6D6BD1DE8A5571489398B392A38A71579D28E311@xmdb04.nch.kids> Hi Mike, Try the butchers (liver, kidney, tripe, heart, brain etc) - very cheap Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Sent: Friday, 26 July 2013 11:09 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Tissue Where can I buy fresh tissue samples to test on my processor? Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From sarah.cosgrove <@t> spotimaging.com Fri Jul 26 07:35:01 2013 From: sarah.cosgrove <@t> spotimaging.com (Sarah Cosgrove) Date: Fri Jul 26 07:35:31 2013 Subject: [Histonet] Tissue In-Reply-To: References: Message-ID: We get fresh samples for testing from the butcher at our local polish market. Best regards, Sarah Cosgrove *SPOT Imaging Solutions*, a division of Diagnostic Instruments, Inc. 6540 Burroughs Ave. Sterling Heights, MI 48314-2133 USA Phone: +1 (586) 731-6000 Fax: +1 (586) 731-6469 Web: www.spotimaging.com * * On Thu, Jul 25, 2013 at 9:08 PM, Mike wrote: > Where can I buy fresh tissue samples to test on my processor? > > Thanks > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Ronald.Houston <@t> nationwidechildrens.org Fri Jul 26 08:41:48 2013 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Fri Jul 26 08:41:55 2013 Subject: [Histonet] RE: Transcriptionist Productivity (Willis, Donna G.) In-Reply-To: <938F8EC5A524D34EB5796E23E52781D361C40369A4@NADCWPMSGCMS05.hca.corpad.net> References: <938F8EC5A524D34EB5796E23E52781D361C40369A4@NADCWPMSGCMS05.hca.corpad.net> Message-ID: Beth surely not 160 characters per hour; perhaps 160 lines/hour? This is one of the measurements of medical transcription productivity standards, typically rates fall within the 100-200 lines/hour, based on 65 characters/line, and depending on the speciality, type of facility and the dictator! The other major standard being Accuracy with a goal of ~98%. My pet hamster could do more than 160 characters per hour and he's arthritic (accuracy probably down in the 0.98% range though)! Ronnie -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Beth.Fye@HCAhealthcare.com Sent: Thursday, July 25, 2013 4:29 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Transcriptionist Productivity (Willis, Donna G.) We have two types of transcriptionists working for our Market Pathology departments. There are onsite transcriptionists that transcribe and have many other duties such as doing send outs and answering phones. Because they have other miscellaneous duties, coming up with a standard was difficult, but we did do it. We compare their characters typed by hour worked. I add up all the character totals and then divide it between the number of transcriptionists. This is the average. Each transcriptionist must be doing at least 80% of the average. This helps to make sure that one transcriptionist is not doing less than others. We also have transcriptionists that do transcription from home. Since they do not have the interruptions or other duties, they have stricter guidelines. The minimum that they are supposed to complete is 160 characters per hour worked however they often do much more. I hope this helps, it's not an easy thing to keep track of, I know. Let me know if you need any more information. Beth A. Fye, CT (ASCP) Pathology Technical Manager HCA Richmond Hospital Laboratories office: (804) 228-6564 fax: (877)699-3895 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Beth.Fye <@t> HCAhealthcare.com Fri Jul 26 08:44:09 2013 From: Beth.Fye <@t> HCAhealthcare.com (Beth.Fye@HCAhealthcare.com) Date: Fri Jul 26 08:44:16 2013 Subject: [Histonet] RE: Transcriptionist Productivity (Willis, Donna G.) In-Reply-To: References: <938F8EC5A524D34EB5796E23E52781D361C40369A4@NADCWPMSGCMS05.hca.corpad.net> Message-ID: <938F8EC5A524D34EB5796E23E52781D361C4036DFD@NADCWPMSGCMS05.hca.corpad.net> I apologize, it is 160 lines per hour. Beth A. Fye, CT (ASCP) Pathology Technical Manager HCA Richmond Hospital Laboratories office: (804) 228-6564 fax: (877)699-3895 -----Original Message----- From: Houston, Ronald [mailto:Ronald.Houston@nationwidechildrens.org] Sent: Friday, July 26, 2013 9:42 AM To: Fye Beth; histonet@lists.utsouthwestern.edu Subject: RE: Transcriptionist Productivity (Willis, Donna G.) Beth surely not 160 characters per hour; perhaps 160 lines/hour? This is one of the measurements of medical transcription productivity standards, typically rates fall within the 100-200 lines/hour, based on 65 characters/line, and depending on the speciality, type of facility and the dictator! The other major standard being Accuracy with a goal of ~98%. My pet hamster could do more than 160 characters per hour and he's arthritic (accuracy probably down in the 0.98% range though)! Ronnie -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Beth.Fye@HCAhealthcare.com Sent: Thursday, July 25, 2013 4:29 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Transcriptionist Productivity (Willis, Donna G.) We have two types of transcriptionists working for our Market Pathology departments. There are onsite transcriptionists that transcribe and have many other duties such as doing send outs and answering phones. Because they have other miscellaneous duties, coming up with a standard was difficult, but we did do it. We compare their characters typed by hour worked. I add up all the character totals and then divide it between the number of transcriptionists. This is the average. Each transcriptionist must be doing at least 80% of the average. This helps to make sure that one transcriptionist is not doing less than others. We also have transcriptionists that do transcription from home. Since they do not have the interruptions or other duties, they have stricter guidelines. The minimum that they are supposed to complete is 160 characters per hour worked however they often do much more. I hope this helps, it's not an easy thing to keep track of, I know. Let me know if you need any more information. Beth A. Fye, CT (ASCP) Pathology Technical Manager HCA Richmond Hospital Laboratories office: (804) 228-6564 fax: (877)699-3895 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mhale <@t> MiracaLS.com Fri Jul 26 08:44:51 2013 From: mhale <@t> MiracaLS.com (Hale, Meredith) Date: Fri Jul 26 08:44:55 2013 Subject: [Histonet] Louisiana HT Position Message-ID: <0E828EC51C7CC445A51E53F81B64E8C72D59AD@s-irv-exchmb.PathologyPartners.intranet> Great full time opportunity for a Histotechnician in Shreveport, Louisiana ! Regional Urology is looking for a certified HT or HTL to join their laboratory . Candidate must meet the following criteria: * Meet CLIA Grossing Requirements : CFR 493.1489, http://wwwn.cdc.gov/clia/regs/toc.aspx/ ,prior experience grossing GI specimens * HT ASCP Certified Duties include: * Grossing * Cytology preparation * Embedding * Microtomy * Staining * Ability to be flexible and take on additional duties' as needed This is a full time position that offers a competitive rate and flexible hours. Interested applicants should contact Meredith Hale; phone 214-596-2219 or through email mhale@miracals.com Meredith Hale HT (ASCP)cm Director External Sales Support Miraca Life Sciences 6655 North MacArthur Blvd. Irving , Texas 75039 Office: 214-596-2219 Cell: 469-648-8253 Fax: 1-866-688-3280 mhale@miracals.com> From s.winville <@t> yahoo.com Fri Jul 26 12:21:08 2013 From: s.winville <@t> yahoo.com (Suzanne Winville) Date: Fri Jul 26 12:21:13 2013 Subject: [Histonet] Tanner Cryostats Message-ID: <1374859268.94066.YahooMailNeo@web160703.mail.bf1.yahoo.com> I am inquiring to anyone that has heard of the Tanner cryostat. I have a colleague that is budgeting for a new unit for their Mohs lab and is familiar with the new Leica CM 1860, Thermo-Fisher HM525. She saw that Mercedes Medical sells the Tanner unit that is apparently competitive in price to more familiar units out in the market. I have done my own due dilligence and discovered that the Tanner unit looks exactly like a unit sold by a Chinese company. The website is www.syroundfin.com. I am very skeptical of companies outside the US that do not have a presence here. Service, parts availability and quality is usually a major obstacle as new distributors in the US do not have an extensive enough service organization to uphold the warrantees. My advice would be to purchase from an established patholgy company that has a worldwide presence. Does anyone have any experience with this unit and suggestions for alternative models? Any feedback would be greatly appreciated! Suzanne Winville From delsuec <@t> gmail.com Fri Jul 26 16:09:18 2013 From: delsuec <@t> gmail.com (Deloris Carter) Date: Fri Jul 26 16:09:24 2013 Subject: [Histonet] Losing tissue on IHC slides Message-ID: Hi all, I'm looking for some troubleshooting help. In the last couple of months we've been having an increasing trend in losing tissue from our IHC slides. We use a Ventana Benchmark XT. PM is done on it quarterly. The problem seems to be with the patient tissue, not the control tissue. We use Fisherbrand Superfrost Plus slides, picking up the control by dipping the top end of the slide to avoid "double dipping". We use tap water in the waterbaths which I know could cause an issue, but that's the way it's always been done here, so there's been no change to that part of the procedure. (We did try using DI water, but it didn't make any difference. Tissue still fell off of slides.) We do use DI water to mix the bulk reagents.The control slides may sometimes have been cut for a longer than recommended time, but that part of the procedure hasn't changed either, same process as always. If we run separate slides for control and patient tissue, we get much better results, but still have some tissue loss. I can't say it's all of our tests, but it is a steadily worsening problem. I'm going through tons of reagent, and I can't seem to nail down the problem. Ventana rep said to try DI water, don't double dip, try adhesive slides (which didn't really make any difference that we could tell). I would appreciate any suggestions Deloris Carter Shawnee Mission Medical Center From tony.auge <@t> gmail.com Fri Jul 26 16:38:00 2013 From: tony.auge <@t> gmail.com (Tony Auge) Date: Fri Jul 26 16:38:04 2013 Subject: [Histonet] Losing tissue on IHC slides In-Reply-To: References: Message-ID: How are you drying your slides? If in an oven, what's the temp and duration? Try going longer and tap off excess water before drying. Tony Auge HTL (ASCP) QIHC Cell: (651) 373-4768 Email: tony.auge@gmail.com From hans <@t> histologistics.com Fri Jul 26 18:24:24 2013 From: hans <@t> histologistics.com (Hans B Snyder) Date: Fri Jul 26 18:24:30 2013 Subject: [Histonet] Losing tissue on IHC slides In-Reply-To: References: Message-ID: I agree water is always a potential issue. Tissue can be finicky though. Have you tried poly-L-lysine coated slides? They do help is some occasions. If you need a protocol, let me know, I can write one up, very easy. Hans B Snyder Histologistics 60 Prescott Street Worcester, MA 01605 508-308-7800 hans@histologistics.com On Fri, Jul 26, 2013 at 5:38 PM, Tony Auge wrote: > How are you drying your slides? If in an oven, what's the temp and > duration? Try going longer and tap off excess water before drying. > > > > Tony Auge HTL (ASCP) QIHC > Cell: (651) 373-4768 > Email: tony.auge@gmail.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From benjamin <@t> histologistics.com Sat Jul 27 06:20:41 2013 From: benjamin <@t> histologistics.com (Benjamin) Date: Sat Jul 27 06:20:58 2013 Subject: [Histonet] Losing tissue on IHC slides In-Reply-To: References: Message-ID: So the patient tissue and control are on the same slide, and the control is staying on but patient tissue is falling off? Maybe dont bake the control tissue just let them air dry, the humidity in the slide drying oven might be messing with the super frost plus slides. Its just a coating on the slide of some sort, i know after the first dip they dont work again like the first time, so just air dry the controls and bake after patient tissue is picked up. Sent from my iPhone On Jul 26, 2013, at 5:09 PM, Deloris Carter wrote: > Hi all, > I'm looking for some troubleshooting help. In the last couple of months > we've been having an increasing trend in losing tissue from our IHC > slides. We use a Ventana Benchmark XT. PM is done on it quarterly. The > problem seems to be with the patient tissue, not the control tissue. We > use Fisherbrand Superfrost Plus slides, picking up the control by dipping > the top end of the slide to avoid "double dipping". We use tap water in > the waterbaths which I know could cause an issue, but that's the way it's > always been done here, so there's been no change to that part of the > procedure. (We did try using DI water, but it didn't make any difference. > Tissue still fell off of slides.) We do use DI water to mix the bulk > reagents.The control slides may sometimes have been cut for a longer than > recommended time, but that part of the procedure hasn't changed either, > same process as always. If we run separate slides for control and patient > tissue, we get much better results, but still have some tissue loss. I > can't say it's all of our tests, but it is a steadily worsening problem. > I'm going through tons of reagent, and I can't seem to nail down the > problem. Ventana rep said to try DI water, don't double dip, try adhesive > slides (which didn't really make any difference that we could tell). I > would appreciate any suggestions > Deloris Carter > Shawnee Mission Medical Center > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From catherinesimonson <@t> gmail.com Sat Jul 27 13:36:29 2013 From: catherinesimonson <@t> gmail.com (Catherine Simonson) Date: Sat Jul 27 13:36:34 2013 Subject: [Histonet] Losing tissue on slides. Message-ID: Hello there, One other possibility, all else being equal, is that of fixation. If whomever is doing the grossing is not fixing the tissue long enough before processing ( do you have a new PA or pathologist?). That definitely has an impact, I would start there. This is especially true of more fatty tissues such as breast tissue and lymph nodes. I would still recommend using DI water in the bath, stand the slides upright after picking up sections for at least 10 to 15 minutes then dry in an oven at 60 degrees Celsius for another 15 to 20 minutes. Just my two cents. Best of luck. Catherine Simonson, B.S. HT(ASCP) From CThornton <@t> dahlchase.com Sun Jul 28 08:24:17 2013 From: CThornton <@t> dahlchase.com (Clare Thornton) Date: Sun Jul 28 08:24:21 2013 Subject: [Histonet] Losing tissue on IHC slides In-Reply-To: References: Message-ID: I have a couple of things. First off, are the sections coming off the slides entirely, or does it have a "chewed up" appearance? If the sections are coming completely off, I would agree with the others that water is the issue. Also, make sure you are cutting no thicker than 4 microns. We had repeated a test twice due to sections falling off, and the tech who cut them has a tendency to cut on 5 microns. I cut them the third time at 4 microns, and let the sections really flatten out on the water bath for a minute. Then baked them well, and third time was the charm. The sections remained on. As far as the "double dipping" of precut control slides go, I spoke directly with a representative from Erie (the makers of all superfrost plus slides) and was told you could dip those slides a hundred times in water, it will not affect the charge on the slides. We've never worried about double dipping and it has never caused us a problem. If the sections have more of a chewed up appearance, then it's probably a combination of factors: fatty tissue, not well fixed, strong antigen retrieval. We've done something for years now that seems to help. After baking, we "post fix" some of our IHC slides in formalin for about 15 minutes. Rinse in tap water then put on stainer as usual. This seems to help when certain antibodies like to chew up the tissue. We don't do this will all antibodies, mostly breast markers and those with strong antigen retrieval. I hope this helps! Good luck. Clare J. Thornton, HTL(ASCP) Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Deloris Carter [delsuec@gmail.com] Sent: Friday, July 26, 2013 5:09 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Losing tissue on IHC slides Hi all, I'm looking for some troubleshooting help. In the last couple of months we've been having an increasing trend in losing tissue from our IHC slides. We use a Ventana Benchmark XT. PM is done on it quarterly. The problem seems to be with the patient tissue, not the control tissue. We use Fisherbrand Superfrost Plus slides, picking up the control by dipping the top end of the slide to avoid "double dipping". We use tap water in the waterbaths which I know could cause an issue, but that's the way it's always been done here, so there's been no change to that part of the procedure. (We did try using DI water, but it didn't make any difference. Tissue still fell off of slides.) We do use DI water to mix the bulk reagents.The control slides may sometimes have been cut for a longer than recommended time, but that part of the procedure hasn't changed either, same process as always. If we run separate slides for control and patient tissue, we get much better results, but still have some tissue loss. I can't say it's all of our tests, but it is a steadily worsening problem. I'm going through tons of reagent, and I can't seem to nail down the problem. Ventana rep said to try DI water, don't double dip, try adhesive slides (which didn't really make any difference that we could tell). I would appreciate any suggestions Deloris Carter Shawnee Mission Medical Center _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SHUNTER <@t> beaumont.edu Mon Jul 29 07:37:08 2013 From: SHUNTER <@t> beaumont.edu (Sue Hunter) Date: Mon Jul 29 07:37:18 2013 Subject: [Histonet] Losing tissue on IHC slides In-Reply-To: References: Message-ID: Good Morning Another thing to try is different slides. We have had issues with bad batches/lots of slides - even the Fisher Superfrost plus. Especially if your purchasing department buys in quantity and then stores the slides in hot warehouses. This seems to negate the charge on the slides - but in an inconsistent way. You can have bad slides mixed with good ones in the same box. Even if you don't want to change vendors, trying a different kind of charged slide would eliminate bad slides as part of the problem. And just an FYI- we do our control slides the same way you do, (and use tap water) so I don't think that is your problem. We are using the "cheaper" Fisher Superfrost plus slides and they seem to work as well as the full priced ones. We cut our immuno sections at 3um. I know how frustrating this can be - I feel your pain! Good luck! Sue Sue Hunter, Supervisor Advanced Diagnostics Beaumont Health System Royal Oak MI 248-898-5146 shunter@beaumont.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Deloris Carter Sent: Friday, July 26, 2013 5:09 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Losing tissue on IHC slides Hi all, I'm looking for some troubleshooting help. In the last couple of months we've been having an increasing trend in losing tissue from our IHC slides. We use a Ventana Benchmark XT. PM is done on it quarterly. The problem seems to be with the patient tissue, not the control tissue. We use Fisherbrand Superfrost Plus slides, picking up the control by dipping the top end of the slide to avoid "double dipping". We use tap water in the waterbaths which I know could cause an issue, but that's the way it's always been done here, so there's been no change to that part of the procedure. (We did try using DI water, but it didn't make any difference. Tissue still fell off of slides.) We do use DI water to mix the bulk reagents.The control slides may sometimes have been cut for a longer than recommended time, but that part of the procedure hasn't changed either, same process as always. If we run separate slides for control and patient tissue, we get much better results, but still have some tissue loss. I can't say it's all of our tests, but it is a steadily worsening problem. I'm going through tons of reagent, and I can't seem to nail down the problem. Ventana rep said to try DI water, don't double dip, try adhesive slides (which didn't really make any difference that we could tell). I would appreciate any suggestions Deloris Carter Shawnee Mission Medical Center _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rooster3801 <@t> yahoo.com Mon Jul 29 07:44:45 2013 From: rooster3801 <@t> yahoo.com (Rooster) Date: Mon Jul 29 07:44:50 2013 Subject: [Histonet] HT jobs in Atlanta? Message-ID: <9258535D-27CD-46C7-8B9B-01062B1308D2@yahoo.com> Hello histoneters! Anyone know of any Histology technician job opportunities in the greater Atlanta GA area? Chris From rooster3801 <@t> yahoo.com Mon Jul 29 07:55:33 2013 From: rooster3801 <@t> yahoo.com (Rooster) Date: Mon Jul 29 07:56:40 2013 Subject: [Histonet] HT jobs in Atlanta Message-ID: <6F178FAC-1761-42D8-ABE4-DC05886A73C2@yahoo.com> Hello Histoneters, Anyone know of any job openings for a registered and experienced histology technician in the greater Atlanta GA area? Thanks Everyone, Chris From KGoodkowsky <@t> goodwin.edu Mon Jul 29 08:01:10 2013 From: KGoodkowsky <@t> goodwin.edu (Kelli Goodkowsky) Date: Mon Jul 29 08:01:20 2013 Subject: [Histonet] Used Tissue Processor Message-ID: <028E6C53598F944BA638B7239290AE4A2A5B61D2@GSEXC100.goodwincollege.org> Good Morning, I am looking to purchase a used tissue processor in good working condition for our student laboratory. Please contact me if you have something. I would like to have a tissue processor for the fall semester if at all possible. Thank you. Kelli Goodkowsky Director Clinical Education Histologic Sciences Goodwin College (860) 727-6917 kgoodkowsky@goodwin.edu From Vickroy.Jim <@t> mhsil.com Mon Jul 29 08:05:15 2013 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Mon Jul 29 08:05:28 2013 Subject: [Histonet] Looking for input on tissue processors Message-ID: We are evaluating tissue processors to replace one of our 1980's VIP. So far we have looked at the Thermofisher STP420, Leica ASP6025, VIP6, and we may look at theThermofisher Excelsior ES. I would be interested in the opinions of other customers which processor they would go with and why. James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From PAMarcum <@t> uams.edu Mon Jul 29 08:24:04 2013 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Mon Jul 29 08:24:09 2013 Subject: [Histonet] RE: Looking for input on tissue processors In-Reply-To: References: Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA32CA17530E@Mail2Node2.ad.uams.edu> Hi Jim, We have four Excelsiors here at UAMS in the Histology Lab. We bought two to replace very old VIPs over 2 years ago and were very happy with the processing and the safety. We have less exposure to fumes as well as ease of use. As supervisor I am concerned about safety. If I find something that will meet or exceed our needs while improving safety and reduce my reagent costs I will go for it. The Excelsiors, especially since we now have replaced all except our bone marrow processor have reduced our cost by about 60% for processing. We have had very few problems and they have been very responsive to us on any questions. Training was excellent in Kalamazoo. Pam -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Monday, July 29, 2013 8:05 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Looking for input on tissue processors We are evaluating tissue processors to replace one of our 1980's VIP. So far we have looked at the Thermofisher STP420, Leica ASP6025, VIP6, and we may look at theThermofisher Excelsior ES. I would be interested in the opinions of other customers which processor they would go with and why. James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From Terri.Brown <@t> Northside.com Mon Jul 29 10:38:52 2013 From: Terri.Brown <@t> Northside.com (Terri Brown) Date: Mon Jul 29 10:39:07 2013 Subject: [Histonet] HT jobs in Atlanta? In-Reply-To: <9258535D-27CD-46C7-8B9B-01062B1308D2@yahoo.com> References: <9258535D-27CD-46C7-8B9B-01062B1308D2@yahoo.com> Message-ID: <731941C266951A47BEF11E5EFAAED9C9196551A5@nsmvexch01.northside.local> Northside Hospital- Histology Supervisor Histology Tech II Histology Tech I Terri H. Brown,, HT (ASCP) Pathology Laboratory Manager Northside Hospital Atlanta terri.brown@northside.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rooster Sent: Monday, July 29, 2013 8:45 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HT jobs in Atlanta? Hello histoneters! Anyone know of any Histology technician job opportunities in the greater Atlanta GA area? Chris _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ************* Begin CareBridge/Ironport DKIM Verification Failure Disclaimer ************* This email has already passed extensive Anti-Spam and Anti-Virus scanning measures and found to have a high probability of not containing spam or virus content. However... This email has failed Domain Keys Identified Mail (DKIM) verification. DKIM references can be found at http://www.dkim.org and is defined by the overseeing organization as: "DomainKeys Identified Mail (DKIM) lets an organization take responsibility for a message while it is in transit. The organization is a handler of the message, either as its originator or as an intermediary. Their reputation is the basis for evaluating whether to trust the message for delivery. 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X-BeenThere: histonet@lists.utsouthwestern.edu X-Mailman-Version: 2.1.5 Precedence: list List-Id: For the exchange of information pertaining to histotechnology and related fields List-Unsubscribe: , List-Archive: List-Post: List-Help: List-Subscribe: , Sender: histonet-bounces@lists.utsouthwestern.edu Errors-To: histonet-bounces@lists.utsouthwestern.edu X-Scan-Signature: fa1887bb5270b51defc3a2885a1d8a92 X-SA-Exim-Connect-IP: 127.0.0.1 X-SA-Exim-Mail-From: histonet-bounces@lists.utsouthwestern.edu X-SA-Exim-Scanned: No (on swlx162.swmed.edu); SAEximRunCond expanded to false ************* End of Disclaimer ************* CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by Northside Hospital. It may contain information that is confidential, privileged, proprietary, or otherwise legally exempt from disclosure. If you are not the intended recipient, you are hereby notified that you are not authorized to read, print, retain, copy or disseminate this message, any part of it, or any attachments. If you have received this message in error, please delete this message and any attachments from your system without reading the content and notify the sender immediately of the inadvertent transmission. There is no intent on the part of the sender to waive any privilege. From lcolbert <@t> pathmdlabs.com Mon Jul 29 10:46:57 2013 From: lcolbert <@t> pathmdlabs.com (Laurie Colbert) Date: Mon Jul 29 10:49:31 2013 Subject: [Histonet] Recycled Xylene Message-ID: <12ECD7346266D74691EC2BFC75285E452F315AB9@BFL323E10.pathmdlabs.local> Does anyone out there have any problems with using recycled xylene to clean the tissue processor? I was just told that we should not use recycled xylene to clean the tissue processor. How about using recycled xylene for either processing or staining? Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab From rjbuesa <@t> yahoo.com Mon Jul 29 11:01:07 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Jul 29 11:01:12 2013 Subject: [Histonet] Recycled Xylene In-Reply-To: <12ECD7346266D74691EC2BFC75285E452F315AB9@BFL323E10.pathmdlabs.local> References: <12ECD7346266D74691EC2BFC75285E452F315AB9@BFL323E10.pathmdlabs.local> Message-ID: <1375113667.52151.YahooMailNeo@web163103.mail.bf1.yahoo.com> I used recycled xylene for any and all tasks where "pure" xylene was needed. Ren? J. ________________________________ From: Laurie Colbert To: "Histonet Post (histonet@lists.utsouthwestern.edu)" Sent: Monday, July 29, 2013 11:46 AM Subject: [Histonet] Recycled Xylene Does anyone out there have any problems with using recycled xylene to? clean the tissue processor?? I was just told that we should not use recycled xylene to clean the tissue processor.? How about using recycled xylene for either processing or staining? Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA? 90048 (323) 648-3214 direct (424) 245-7284 main lab _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From flnails <@t> texaschildrens.org Mon Jul 29 11:02:07 2013 From: flnails <@t> texaschildrens.org (Nails, Felton) Date: Mon Jul 29 11:02:13 2013 Subject: [Histonet] RE: Recycled Xylene In-Reply-To: <12ECD7346266D74691EC2BFC75285E452F315AB9@BFL323E10.pathmdlabs.local> References: <12ECD7346266D74691EC2BFC75285E452F315AB9@BFL323E10.pathmdlabs.local> Message-ID: <327E034F1892504289B7A17EC71DF9F30231EE@TCFMSG03.ad.texaschildrenshospital.org> We have been using recycled xylene for years and have found that it only present a problem on the clean cycle of the processor. Everywhere we have not experienced a problem, so we use new xylene on the cleaning cycle. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Monday, July 29, 2013 10:47 AM To: Histonet Post (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Recycled Xylene Does anyone out there have any problems with using recycled xylene to clean the tissue processor? I was just told that we should not use recycled xylene to clean the tissue processor. How about using recycled xylene for either processing or staining? Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ______________________________________________________________________ From ctorrence <@t> kmcpa.com Mon Jul 29 11:16:17 2013 From: ctorrence <@t> kmcpa.com (Carol Torrence) Date: Mon Jul 29 11:18:17 2013 Subject: [Histonet] Looking for cryomolds Message-ID: <7A588CEA30D05743AA8F65E38BEC6E7C86F56B@KMC1Exc.kmcpa.com> I found an old box of Tissue Tek 4728 cryomolds in our Mohs lab. They are round with a small tab on the side. We like them so much better than the disposable embedding molds sold for paraffin embedding. I have seen cryomolds that look like the same ones for paraffin but are they any thinner for quicker freezing? 1. Does anyone know where to get the round ones? I do not see them on the Sakura website. 2. Do the square ones that look like our paraffin molds release easily? Thanks, Carol Torrence From m_chadwell <@t> hotmail.com Mon Jul 29 11:23:59 2013 From: m_chadwell <@t> hotmail.com (margaret chadwell) Date: Mon Jul 29 11:25:06 2013 Subject: =?utf-8?Q?Re:_[Histonet]_Looking_for_cryomolds?= Message-ID: Hi Carol PSL (Pacific Southwest Lab Supplies) sells the round Cryomolds. Margie Chadwell Sent from Windows Mail From: Carol Torrence Sent: ?Monday?, ?July? ?29?, ?2013 ?9?:?18? ?AM To: histonet@lists.utsouthwestern.edu I found an old box of Tissue Tek 4728 cryomolds in our Mohs lab. They are round with a small tab on the side. We like them so much better than the disposable embedding molds sold for paraffin embedding. I have seen cryomolds that look like the same ones for paraffin but are they any thinner for quicker freezing? 1. Does anyone know where to get the round ones? I do not see them on the Sakura website. 2. Do the square ones that look like our paraffin molds release easily? Thanks, Carol Torrence _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dmccaig <@t> ckha.on.ca Mon Jul 29 11:54:34 2013 From: dmccaig <@t> ckha.on.ca (Diana McCaig) Date: Mon Jul 29 11:55:45 2013 Subject: [Histonet] Looking for cryomolds In-Reply-To: References: Message-ID: I did not know such a product existed until now. How do these molds work? Diana McCaig Histology Lab Chatham Kent Health Alliance 80 Grand Avenue West Chatham. Ontario N7L 1B7 519-352-6401 (6604) This email communication and any files transmitted with it may contain confidential and or proprietary information and is provided for the use of the intended recipient only. Any review, retransmission or dissemination of this information by anyone other than the intended recipient is prohibited. If you receive this email in error, please contact the sender and delete this communication and any copies immediately. Thank you. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of margaret chadwell Sent: July-29-13 12:24 PM To: histonet@lists.utsouthwestern.edu; Carol Torrence Subject: Re: [Histonet] Looking for cryomolds Hi Carol PSL (Pacific Southwest Lab Supplies) sells the round Cryomolds. Margie Chadwell Sent from Windows Mail From: Carol Torrence Sent: ?Monday?, ?July? ?29?, ?2013 ?9?:?18? ?AM To: histonet@lists.utsouthwestern.edu I found an old box of Tissue Tek 4728 cryomolds in our Mohs lab. They are round with a small tab on the side. We like them so much better than the disposable embedding molds sold for paraffin embedding. I have seen cryomolds that look like the same ones for paraffin but are they any thinner for quicker freezing? 1. Does anyone know where to get the round ones? I do not see them on the Sakura website. 2. Do the square ones that look like our paraffin molds release easily? Thanks, Carol Torrence _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BGapinski <@t> pathgroup.com Mon Jul 29 12:02:43 2013 From: BGapinski <@t> pathgroup.com (Bruce Gapinski) Date: Mon Jul 29 12:02:48 2013 Subject: [Histonet] AM HT Message-ID: Early shift available. We are looking for someone to embed starting at 2:30 AM in gorgeous Marin County California. HT preferred, but will train if necessary. To apply contact Bruce Gapinski bgapinski@pathgroup.com (415) 209-6076 Fax resumes to (415) 898-0870 Bruce Gapinsk HT (ASCP) Chief Histologist Marin Medical Laboratories PathGroup SF ________________________________ Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you From Dorothy.L.Webb <@t> HealthPartners.Com Mon Jul 29 12:11:56 2013 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Mon Jul 29 12:12:11 2013 Subject: [Histonet] used xylene/VIP 6 Message-ID: <65365F35C0F2EF4D846EC3CA73E49C43029A796FEE18@HPEMX3.HealthPartners.int> We have the VIP 6 and really like it..user friendly and faster TAT. We have been having rotary valve concerns within our Leica Peloris tissue processor and the technician as well as Leica technical services both feel it is because we have been using recycled xylene in the cleaning cycle. I was always told that when recycling xylene, one gets a "pure" product back, which I always found hard to believe. We do use recycled xylene in routine tissue processing, rotating between new and recycled as we change the reagents and rotate. Dorothy Webb, HT (ASCP) Regions Histology Technical Specialist ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 From CDavis <@t> che-east.org Mon Jul 29 12:30:22 2013 From: CDavis <@t> che-east.org (Davis, Cassie) Date: Mon Jul 29 12:30:38 2013 Subject: [Histonet] RE: cryomolds In-Reply-To: <84a5c26a-acec-41a3-9144-e113180fde36@CHESXH01.one.ads.che.org> References: <84a5c26a-acec-41a3-9144-e113180fde36@CHESXH01.one.ads.che.org> Message-ID: <08861B9CF6C7774E874635A4818AE37B0124310777@CHEXCMS01.one.ads.che.org> The cryomold have been moved on the website: check under specification & accessories of the cryostats. I hate when they move things on websites but I guess they are employing the grocery store theory (move thing so customers will see other stuff to buy as they look for what they really need) http://www.sakura-americas.com/products/tisstek-cryo3mc.html My experience with the square & rectangle ones, they are ok for about 1-2 uses with the paraffin, then the centers warp. We did freeze some of our case tissue to save for long term storage in the square ones with OTC but we didn't have to cut the tissues Cassandra Davis CDavis@che-east.org 302-575-8095 ________________________________________ Message: 11 Date: Mon, 29 Jul 2013 16:16:17 +0000 From: Carol Torrence Subject: [Histonet] Looking for cryomolds To: "histonet@lists.utsouthwestern.edu" Message-ID: <7A588CEA30D05743AA8F65E38BEC6E7C86F56B@KMC1Exc.kmcpa.com> Content-Type: text/plain; charset="us-ascii" I found an old box of Tissue Tek 4728 cryomolds in our Mohs lab. They are round with a small tab on the side. We like them so much better than the disposable embedding molds sold for paraffin embedding. I have seen cryomolds that look like the same ones for paraffin but are they any thinner for quicker freezing? 1. Does anyone know where to get the round ones? I do not see them on the Sakura website. 2. Do the square ones that look like our paraffin molds release easily? Thanks, Carol Torrence Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From rjbuesa <@t> yahoo.com Mon Jul 29 15:07:32 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Jul 29 15:07:41 2013 Subject: [Histonet] used xylene/VIP 6 In-Reply-To: <65365F35C0F2EF4D846EC3CA73E49C43029A796FEE18@HPEMX3.HealthPartners.int> References: <65365F35C0F2EF4D846EC3CA73E49C43029A796FEE18@HPEMX3.HealthPartners.int> Message-ID: <1375128452.56022.YahooMailNeo@web163105.mail.bf1.yahoo.com> Let me be as frank and cynical as possible: properly recycled xylene will produce xylene?pure and "unadulterated". Those "opinions"?from "technical" services?that also provide non-recycled xylene have a vested interest in making you believe that their product is the ONLY one to use. Never believe somebody who has a vested interest in selling you something when providing an opinion against something you obtain cheaper. Just my "cynical" opinion (usually right on these issues). Otherwise, provide scientific proof to the contrary! I used recycled xylene in my 3 VIP and added pure xylene when my reserves were exhausted or depleted by use. Ren? J. ________________________________ From: "Webb, Dorothy L" To: "'histonet@lists.utsouthwestern.edu'" Sent: Monday, July 29, 2013 1:11 PM Subject: [Histonet] used xylene/VIP 6 We have the VIP 6 and really like it..user friendly and faster TAT. We have been having rotary valve concerns within our Leica Peloris tissue processor and the technician as well as Leica technical services both feel it is because we have been using recycled xylene in the cleaning cycle.? I was always told that when recycling xylene, one gets a "pure" product back, which I always found hard to believe.? We do use recycled xylene in routine tissue processing, rotating between new and recycled as we change the reagents and rotate. Dorothy Webb, HT (ASCP) Regions Histology Technical Specialist ? ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Mon Jul 29 15:24:13 2013 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Mon Jul 29 15:24:18 2013 Subject: [Histonet] Looking for cryomolds In-Reply-To: <7A588CEA30D05743AA8F65E38BEC6E7C86F56B@KMC1Exc.kmcpa.com> References: <7A588CEA30D05743AA8F65E38BEC6E7C86F56B@KMC1Exc.kmcpa.com> Message-ID: I know the round ones you are talking about. I like them too. The other square shaped are also good. I have some order # 4566, and they are about the dimensions of a metal biopsy base mold with a extended edge. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: ctorrence@kmcpa.com > To: histonet@lists.utsouthwestern.edu > Date: Mon, 29 Jul 2013 16:16:17 +0000 > Subject: [Histonet] Looking for cryomolds > > I found an old box of Tissue Tek 4728 cryomolds in our Mohs lab. They are round with a small tab on the side. We like them so much better than the disposable embedding molds sold for paraffin embedding. I have seen cryomolds that look like the same ones for paraffin but are they any thinner for quicker freezing? > > 1. Does anyone know where to get the round ones? I do not see them on the Sakura website. > 2. Do the square ones that look like our paraffin molds release easily? > > Thanks, > Carol Torrence > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From yyung <@t> scripps.edu Mon Jul 29 18:00:27 2013 From: yyung <@t> scripps.edu (Yun Yung) Date: Mon Jul 29 18:00:31 2013 Subject: [Histonet] Histochoice MB vs other fixatives Message-ID: <9EB1B123C28EA4488A6D524DAF09F32D023A5BC215ED@EXCH-CCR01.lj.ad.scripps.edu> Dear Histoland, Does anyone have experience using Histochoice for IHC, in particular for mouse brain tissue? Cryoprotected vs paraffinized material? I am currently looking at both structural proteins (e.g., tubulin) as well as transcription factors (e.g., b-catenin and Pax6). I normally use 4% PFA (cryoprotected, frozen, OCT embedded) FAA (formalin-ethanol-acetic acid) (paraffin embedded). These two fixes work decently, but now I'm starting to do IHC for G protein-coupled receptors and some have suggested using PLP as an alternative. There was a small discussion in 2009 on this list; hopefully more people have experience with it now. Thanks, Yun ___________________________________________ Yun C. Yung, Ph.D. Chun Lab, Dorris Neuroscience Center The Scripps Research Institute 10550 N. Torrey Pines Rd, DNC-118 La Jolla, CA 92037 From ggracie <@t> stvincents.com.au Mon Jul 29 23:58:26 2013 From: ggracie <@t> stvincents.com.au (Gary Gracie) Date: Mon Jul 29 23:59:12 2013 Subject: [Histonet] impact of KOH on melanoma markers Message-ID: <9499653da5734fd3af324fed5e3aacee@SVMHSEXCH02.svmhs.stvincents.com.au> Dear All I recently received a request for HMB45, MART-1, MiTF, S100 and Tyrosinase on sections of toe nail. Our laboratory will usually soften the toe nail in Potassium hydroxide (KOH) either before processing or sometimes after trimming the paraffin block and floating the block in a jar of KOH. The pathologist was reasonably certain the nail contained melanoma yet the markers were all negative or at best, weakly postive. Thankfully there was a second specimen that, for whatever reason, had missed the KOH treatment. This stained with the markers as expected. Does anyone else use KOH for nail softening? Has anyone ever encountered a problem with KOH "knocking out" epitopes as described? Thanks Gary Gracie Senior Technical Officer IHC Laboratory Anatomical Pathology St Vincents Hospital Sydney, Australia ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the system manager. This footnote also confirms that this email message has been virus scanned and although no viruses were detected by the system, St Vincents & Mater Health Sydney accepts no liability for any consequential damage resulting from email containing any computer viruses. ********************************************************************** From mhale <@t> MiracaLS.com Tue Jul 30 07:13:38 2013 From: mhale <@t> MiracaLS.com (Hale, Meredith) Date: Tue Jul 30 07:13:43 2013 Subject: [Histonet] Louisiana Position Message-ID: <0E828EC51C7CC445A51E53F81B64E8C72D843F@s-irv-exchmb.PathologyPartners.intranet> Great full time opportunity for a Histotechnician in Shreveport, Louisiana ! Regional Urology is looking for a certified HT or HTL to join their laboratory . Candidate must meet the following criteria: * Meet CLIA Grossing Requirements : CFR 493.1489, http://wwwn.cdc.gov/clia/regs/toc.aspx/ ,prior experience grossing GI specimens * HT ASCP Certified Duties include: * Grossing * Cytology preparation * Embedding * Microtomy * Staining * Ability to be flexible and take on additional duties' as needed This is a full time position that offers a competitive rate and flexible hours. Interested applicants should contact Meredith Hale; phone 214-596-2219 or through email mhale@miracals.com Meredith Hale HT (ASCP)cm Director External Sales Support Miraca Life Sciences 6655 North MacArthur Blvd. Irving , Texas 75039 Office: 214-596-2219 Cell: 469-648-8253 Fax: 1-866-688-3280 mhale@miracals.com> From Stephen.Clark1 <@t> hcahealthcare.com Tue Jul 30 07:31:07 2013 From: Stephen.Clark1 <@t> hcahealthcare.com (Stephen.Clark1@hcahealthcare.com) Date: Tue Jul 30 07:31:15 2013 Subject: [Histonet] Any poitions open in the Charlotte, NC area Message-ID: <213A638666ECDB4A8A81B5CF8646CC0B74E4A9E212@NADCWPMSGCMS05.hca.corpad.net> Hey everyone, Just inquiring as to if anyone knows of any Histotech positions in the Charlotte, NC are. If anyone is familiar with any open positions, please let me know. Thank you. Steve Clark Pathology Dept. Supervisor Grand Strand Regional Medical Center 843-692-1486 Lab 843-692-1459 Desk Stephen.Clark1@hcahealthcare.com [cid:image001.gif@01C9DADA.65BB9E10] From durynski <@t> vet.upenn.edu Tue Jul 30 10:15:40 2013 From: durynski <@t> vet.upenn.edu (Karie Durynski) Date: Tue Jul 30 10:15:49 2013 Subject: [Histonet] Cryosectioning of Rabbit Arteries Message-ID: <1594099375.7451223.1375197340653.JavaMail.root@zimbra.upenn.edu> Good morning, I am sectioning fresh frozen rabbit arteries and they need to be nice and round/oval on the slides before staining. I am trying to float them in PBS but losing the section in PBS and unable to pick up and place on the slide due to the size of the tissue. They are so small to handle this way. If any one has a procedure or any advice they can share it would be greatly appreciated. Thank you much, Karie -- Karie L Durynski A.S. New Bolton Center University of Pennsylvania School of Veterinary Medicine Comparative Orthopaedic Research Laboratory 382 W Street Road Kennett Square, PA. 19348 Phone:610-925-6278 Fax:610-925-6820 Email:durynski@vet.upenn.edu From nto <@t> stowers.org Tue Jul 30 10:37:14 2013 From: nto <@t> stowers.org (Thomas, Nancy) Date: Tue Jul 30 10:37:22 2013 Subject: [Histonet] quality sections of bone using tape transfer method Message-ID: Recently, I have been spending much time using the tape transfer method to get sections of undecalcified mouse femur and humerus. After searching histonet archives, I am using some suggestions such as 4x coated slides, changing the knife angle, flashing several times, rolling the $#*! out of the sections (as was suggested :)) and letting the slides rest on dry ice after flashing them. Thank you because these have all helped the bone tissue to remain on the slide while pulling the tape off. Now my problem is the quality of the bone tissue. Microscopically, it looks like a cobble-stone walkway... many little circular structures all bumping up against each other. It is noticeable on unstained sections, so staining is not the cause. I have made sections between 3? and 10? and it is present on all, although the 3? had one smooth area. I would compare it somewhat to a cryo section of brain that sections nicely, then crackles as it adheres to the slide. I have tried many different ways to get a better section, but have not yet hit on the magic mix. I am at the point of blaming the cryo jane apparatus since it is over 10 years old. We checked into changing the UV lamp but were told that parts are no longer made for this model. We are checking into a new piece of equipment, but first, is there anything I have overlooked? Has anyone seen this same type of feature I have described? Any thoughts as to what could be causing it and how to eliminate it? Thank you in advance for any help and advice, Nancy Thomas Stower's Institute for Medical Research Kansas City, MO From liz <@t> premierlab.com Tue Jul 30 10:48:49 2013 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Tue Jul 30 10:51:26 2013 Subject: [Histonet] RE: quality sections of bone using tape transfer method In-Reply-To: References: Message-ID: <14E2C6176416974295479C64A11CB9AE016411AB4B20@SBS2K8.premierlab.local> Nancy The cryojane system in my opinion is not a perfect system, it works sometimes and other times its not that successful. John Tarpley wrote an article in the JOH a while back (in the 80's or 90's) on his process for undecalcifed bone section utilizing the tape transfer system. It should be in the archives on the NSH website if you are a member. Good Luck Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 881-0763 cell (303) 682-9060 fax liz@premierlab.com Ship to address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Thomas, Nancy [nto@stowers.org] Sent: Tuesday, July 30, 2013 9:37 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] quality sections of bone using tape transfer method Recently, I have been spending much time using the tape transfer method to get sections of undecalcified mouse femur and humerus. After searching histonet archives, I am using some suggestions such as 4x coated slides, changing the knife angle, flashing several times, rolling the $#*! out of the sections (as was suggested :)) and letting the slides rest on dry ice after flashing them. Thank you because these have all helped the bone tissue to remain on the slide while pulling the tape off. Now my problem is the quality of the bone tissue. Microscopically, it looks like a cobble-stone walkway... many little circular structures all bumping up against each other. It is noticeable on unstained sections, so staining is not the cause. I have made sections between 3? and 10? and it is present on all, although the 3? had one smooth area. I would compare it somewhat to a cryo section of brain that sections nicely, then crackles as it adheres to the slide. I have tried many different ways to get a better section, but have not yet hit on the magic mix. I am at the point of blaming the cryo jane apparatus since it is over 10 years old. We checked into changing the UV lamp but were told that parts are no longer made for this model. We are checking into a new piece of equipment, but first, is there anything I have overlooked? Has anyone seen this same type of feature I have described? Any thoughts as to what could be causing it and how to eliminate it? Thank you in advance for any help and advice, Nancy Thomas Stower's Institute for Medical Research Kansas City, MO _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Jul 30 11:31:58 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jul 30 11:32:03 2013 Subject: [Histonet] Cryosectioning of Rabbit Arteries In-Reply-To: <1594099375.7451223.1375197340653.JavaMail.root@zimbra.upenn.edu> References: <1594099375.7451223.1375197340653.JavaMail.root@zimbra.upenn.edu> Message-ID: <1375201918.26631.YahooMailNeo@web163101.mail.bf1.yahoo.com> First you have to make sure they are round before they are placed in OCT. If they are not round at that step it will be very difficult to "round them up" in PBS. If round in the OCT just pick them directly from the blade. Floating them is never a good idea. Ren? J.? ________________________________ From: Karie Durynski To: histonet@lists.utsouthwestern.edu Sent: Tuesday, July 30, 2013 11:15 AM Subject: [Histonet] Cryosectioning of Rabbit Arteries Good morning, I am sectioning fresh frozen rabbit arteries and they need to be nice and round/oval on the slides before staining. I am trying to float them in PBS but losing the section in PBS and unable to pick up and place on the slide due to the size of the tissue. They are so small to handle this way. If any one has a procedure or any advice they can share it would be greatly appreciated. Thank you much, Karie -- Karie L Durynski A.S. New Bolton Center University of Pennsylvania School of Veterinary Medicine Comparative Orthopaedic Research Laboratory 382 W Street Road Kennett Square, PA. 19348 Phone:610-925-6278 Fax:610-925-6820 Email:durynski@vet.upenn.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cpyse <@t> x-celllab.com Tue Jul 30 13:05:44 2013 From: cpyse <@t> x-celllab.com (Cindy Pyse) Date: Tue Jul 30 13:05:56 2013 Subject: [Histonet] PAX-2 Message-ID: <005801ce8d4f$692ad2b0$3b807810$@x-celllab.com> Hi Histonetters I am currently working on the optimization of the PAX-2 antibody (concentrate) from cell marque. Does anyone have a protocol they would be will to share for the Dako48. Thanks to any and all in advance. Cindy Cindy Pyse CLT, HT(ASCP) Laboratory Manager X-Cell Laboratories of WNY 20 Northpointe Parkway Ste 100 Amherst, NY 14228 716-250-9235 Ext. 232 cpyse@x-celllab.com From Elizabeth.Cameron <@t> jax.org Tue Jul 30 14:02:26 2013 From: Elizabeth.Cameron <@t> jax.org (Elizabeth Cameron) Date: Tue Jul 30 14:02:32 2013 Subject: [Histonet] DNA Extraction Message-ID: We are preparing paraffin slides for DNA extraction, and I am not sure if once cut they should be kept cold before the extraction is done. We are not doing the extraction, just the prep. If anyone else is doing this, I would greatly appreciate some input. Thank you, Elizabeth The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as possible. From Bruce_Palmatier <@t> vwr.com Tue Jul 30 14:22:07 2013 From: Bruce_Palmatier <@t> vwr.com (Bruce_Palmatier@vwr.com) Date: Tue Jul 30 14:22:24 2013 Subject: [Histonet] AUTO: Bruce Palmatier is out of the office (returning 08/02/2013) Message-ID: I will be out of the office starting 07/30/2013 and will not return until 08/02/2013 I will be out of the office this week attending the AACC meeting in Houston. If you have a question about an order or need assistance placing an order, or if the matter is urgent, please call 877-881-1192 between 8am and 8pm and a VWR Healthcare Service associate will assist you. For quote requests and pricing-related inquiries, I will respond with 24 hours. Thank You, Bruce Palmatier VWR Healthcare mobile 484-319-5563 Note: This is an automated response to your message "Histonet Digest, Vol 116, Issue 32" sent on 7/30/2013 1:05:30 PM. This is the only notification you will receive while this person is away. From marktarango <@t> gmail.com Tue Jul 30 14:26:08 2013 From: marktarango <@t> gmail.com (Mark Tarango) Date: Tue Jul 30 14:26:17 2013 Subject: [Histonet] DNA Extraction In-Reply-To: References: Message-ID: Hi Elizabeth, Room temp is fine for FFPE tissue for DNA extraction. We do it this way every day! thanks Mark On Tue, Jul 30, 2013 at 12:02 PM, Elizabeth Cameron < Elizabeth.Cameron@jax.org> wrote: > We are preparing paraffin slides for DNA extraction, and I am not sure if > once cut they should be kept cold before the extraction is done. We are > not doing the extraction, just the prep. If anyone else is doing this, I > would greatly appreciate some input. > Thank you, > Elizabeth > > The information in this email, including attachments, may be confidential > and is intended solely for the addressee(s). If you believe you received > this email by mistake, please notify the sender by return email as soon as > possible. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jwood <@t> fairchildmed.org Tue Jul 30 14:37:19 2013 From: jwood <@t> fairchildmed.org (Jean Wood) Date: Tue Jul 30 14:38:20 2013 Subject: [Histonet] Two Patient Identifiers on slides Message-ID: <87A4EF3F1FBA9D42A7D1EC82F031C0B80A3FD35E07@cliff> Hello Histonetters, Recently we started utilizing a slide labeling component that is built into our AP Easy LIS system and has accession number, levels and patients first and last name when labels are printed out. Dymo does not have a chemically resistant label (we have a Dymo 450 printer) and we have been putting the labels on AFTER the slides are stained and cover slipped. In the meantime, the HT is writing in pencil the accession # and levels on the slide which is then covered up with the permanent label after cover slipping. Our Lab Manager is worried that we are not compliant as we do not have two patient identifiers on throughout the whole process (she wants us to write patient names on slides in pencil (before staining) and then cover that up with the pre-printed label after staining. 1. What is everyone else doing? 2. Have any of you found a chemically resistant label compatible with the Dymo labeler? Jean Wood BS, HT Fairchild Medical Center Pathology Dept. Ph:530.841.6243 Fax:530.841.6232 jwood@fairchildmed.org From DKBoyd <@t> chs.net Tue Jul 30 14:41:58 2013 From: DKBoyd <@t> chs.net (Boyd, Debbie M) Date: Tue Jul 30 14:42:07 2013 Subject: [Histonet] RE: Two Patient Identifiers on slides In-Reply-To: <87A4EF3F1FBA9D42A7D1EC82F031C0B80A3FD35E07@cliff> References: <87A4EF3F1FBA9D42A7D1EC82F031C0B80A3FD35E07@cliff> Message-ID: <7EAFE982E328304DA6CE2B677BB76246794ECA94@TN001WEXMBX12.US.chs.net> Your manager is correct. You must have two patient identifiers through the whole process. We write the full name, accession number and designation ( a, b L1 ) on each slide. I have had both CAP and Joint Commission inspectors pull the labels back looking for 2 identifiers. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jean Wood Sent: Tuesday, July 30, 2013 3:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Two Patient Identifiers on slides Hello Histonetters, Recently we started utilizing a slide labeling component that is built into our AP Easy LIS system and has accession number, levels and patients first and last name when labels are printed out. Dymo does not have a chemically resistant label (we have a Dymo 450 printer) and we have been putting the labels on AFTER the slides are stained and cover slipped. In the meantime, the HT is writing in pencil the accession # and levels on the slide which is then covered up with the permanent label after cover slipping. Our Lab Manager is worried that we are not compliant as we do not have two patient identifiers on throughout the whole process (she wants us to write patient names on slides in pencil (before staining) and then cover that up with the pre-printed label after staining. 1. What is everyone else doing? 2. Have any of you found a chemically resistant label compatible with the Dymo labeler? Jean Wood BS, HT Fairchild Medical Center Pathology Dept. Ph:530.841.6243 Fax:530.841.6232 jwood@fairchildmed.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From mmooreht <@t> yahoo.com Tue Jul 30 14:46:03 2013 From: mmooreht <@t> yahoo.com (Michelle Moore) Date: Tue Jul 30 14:46:09 2013 Subject: [Histonet] Two Patient Identifiers on slides In-Reply-To: <87A4EF3F1FBA9D42A7D1EC82F031C0B80A3FD35E07@cliff> References: <87A4EF3F1FBA9D42A7D1EC82F031C0B80A3FD35E07@cliff> Message-ID: <1375213563.32782.YahooMailNeo@web125101.mail.ne1.yahoo.com> We are writing path #, date of birth & patient initials as identifiers.? Michelle ________________________________ From: Jean Wood To: "histonet@lists.utsouthwestern.edu" Sent: Tuesday, July 30, 2013 2:37 PM Subject: [Histonet] Two Patient Identifiers on slides Hello Histonetters, ? ? ? Recently we started utilizing a slide labeling component that is built into our AP Easy LIS system and has accession number, levels and patients first and last name when labels are printed out. Dymo does not have a chemically resistant label (we have a Dymo 450 printer) and we have been putting the labels on AFTER the slides are stained and cover slipped. In the meantime, the HT is writing in pencil the accession # and levels on the slide which is then covered up with the permanent label after cover slipping. Our Lab Manager is worried that we are not compliant as we do not have two patient identifiers on throughout the whole process (she wants us to write patient names on slides in pencil (before staining) and then cover that up with the pre-printed label after staining. 1. What is everyone else doing? 2. Have any of you found a chemically resistant label compatible with the Dymo labeler? Jean Wood BS, HT Fairchild Medical Center Pathology Dept. Ph:530.841.6243 Fax:530.841.6232 jwood@fairchildmed.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MWeirauch <@t> crittenton.com Tue Jul 30 15:04:27 2013 From: MWeirauch <@t> crittenton.com (Maray Weirauch) Date: Tue Jul 30 15:04:31 2013 Subject: [Histonet] Suncycle Technologies Message-ID: Has anyone used this gravity recycling system for their reagent alcohol? Good? Bad? Comments? Thanks! From joewalker <@t> rrmc.org Tue Jul 30 15:30:41 2013 From: joewalker <@t> rrmc.org (Joe W. Walker, Jr.) Date: Tue Jul 30 15:30:57 2013 Subject: [Histonet] Two Patient Identifiers on slides In-Reply-To: <1375213563.32782.YahooMailNeo@web125101.mail.ne1.yahoo.com> References: <87A4EF3F1FBA9D42A7D1EC82F031C0B80A3FD35E07@cliff> <1375213563.32782.YahooMailNeo@web125101.mail.ne1.yahoo.com> Message-ID: <3C2378778400AD448ADA6FD6BDB7CCCC17FFE0FD@RRMBX03.rrmc.local> I don't think this is technically correct according to the CAP. The question in the most recent checklist states: **REVISED** 07/11/2011 ANP.52000 Specimen ID Phase II The identity of every specimen and image, including blocks, slides, and electron micrographs, is maintained through each step in processing. NOTE: Each block of tissue must be individually labeled with unique patient identifier(s), e.g. accession number etched onto the block or embedded into it. Storage of unlabeled blocks in separate containers that are labeled with patient number or name does not meet this requirement. Evidence of Compliance: ? Written procedure describing system for maintaining specimen identity This question does not state that 2 identifiers must be on the slides and blocks, etc. It states it must be a unique identifier throughout the process. A specimen accession number would technically suffice. You can add additional identifiers if that helps in your process but they are not required. I believe that this is often confused with the General lab question GEN.40491 which requires that any specimen that is collected and submitted to the lab must have 2 unique identifiers on it. Once the specimen is in the lab, the unique accession number would suffice. Now, if you are utilizing the 2 identifiers to help prevent mislabeling, then I think it is a good idea as an extra precaution step. We are fortunate to have a xylene resistant label and printer that allows us to label the slides prior to processing. My two cents as a manager, Joe W. Walker, Jr. MS, SCT(ASCP)CM Anatomical Pathology Manager Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P: 802.747.1790 F: 802.747.6525 Email joewalker@rrmc.org www.rrmc.org Our Vision: To be the Best Community Healthcare System in New England Rutland Regional?Vermont?s 1st Hospital to Achieve Both ANCC Magnet Recognition? and the Governor?s Award for Performance Excellence -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michelle Moore Sent: Tuesday, July 30, 2013 3:46 PM To: Jean Wood; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Two Patient Identifiers on slides We are writing path #, date of birth & patient initials as identifiers. Michelle ________________________________ From: Jean Wood To: "histonet@lists.utsouthwestern.edu" Sent: Tuesday, July 30, 2013 2:37 PM Subject: [Histonet] Two Patient Identifiers on slides Hello Histonetters, Recently we started utilizing a slide labeling component that is built into our AP Easy LIS system and has accession number, levels and patients first and last name when labels are printed out. Dymo does not have a chemically resistant label (we have a Dymo 450 printer) and we have been putting the labels on AFTER the slides are stained and cover slipped. In the meantime, the HT is writing in pencil the accession # and levels on the slide which is then covered up with the permanent label after cover slipping. Our Lab Manager is worried that we are not compliant as we do not have two patient identifiers on throughout the whole process (she wants us to write patient names on slides in pencil (before staining) and then cover that up with the pre-printed label after staining. 1. What is everyone else doing? 2. Have any of you found a chemically resistant label compatible with the Dymo labeler? Jean Wood BS, HT Fairchild Medical Center Pathology Dept. Ph:530.841.6243 Fax:530.841.6232 jwood@fairchildmed.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. Thank You From jaylundgren <@t> gmail.com Tue Jul 30 15:46:08 2013 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Tue Jul 30 15:46:22 2013 Subject: [Histonet] Two Patient Identifiers on slides In-Reply-To: <3C2378778400AD448ADA6FD6BDB7CCCC17FFE0FD@RRMBX03.rrmc.local> References: <87A4EF3F1FBA9D42A7D1EC82F031C0B80A3FD35E07@cliff> <1375213563.32782.YahooMailNeo@web125101.mail.ne1.yahoo.com> <3C2378778400AD448ADA6FD6BDB7CCCC17FFE0FD@RRMBX03.rrmc.local> Message-ID: Birth date is not a unique patient identifier. Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) On Tue, Jul 30, 2013 at 3:30 PM, Joe W. Walker, Jr. wrote: > I don't think this is technically correct according to the CAP. The > question in the most recent checklist states: > > **REVISED** 07/11/2011 > > ANP.52000 Specimen ID Phase II > The identity of every specimen and image, including blocks, slides, and > electron micrographs, > is maintained through each step in processing. > > NOTE: Each block of tissue must be individually labeled with unique > patient identifier(s), e.g. > accession number etched onto the block or embedded into it. Storage of > unlabeled blocks in separate > containers that are labeled with patient number or name does not meet this > requirement. > Evidence of Compliance: > > ? Written procedure describing system for maintaining specimen identity > > This question does not state that 2 identifiers must be on the slides and > blocks, etc. It states it must be a unique identifier throughout the > process. A specimen accession number would technically suffice. You can > add additional identifiers if that helps in your process but they are not > required. > > I believe that this is often confused with the General lab question > GEN.40491 which requires that any specimen that is collected and submitted > to the lab must have 2 unique identifiers on it. Once the specimen is in > the lab, the unique accession number would suffice. > > Now, if you are utilizing the 2 identifiers to help prevent mislabeling, > then I think it is a good idea as an extra precaution step. We are > fortunate to have a xylene resistant label and printer that allows us to > label the slides prior to processing. > > My two cents as a manager, > > Joe W. Walker, Jr. MS, SCT(ASCP)CM > Anatomical Pathology Manager > Rutland Regional Medical Center > 160 Allen Street, Rutland, VT 05701 > P: 802.747.1790 F: 802.747.6525 > Email joewalker@rrmc.org www.rrmc.org > > Our Vision: > To be the Best Community Healthcare System in New England > > Rutland Regional?Vermont?s 1st Hospital to Achieve Both ANCC Magnet > Recognition? and the Governor?s Award for Performance Excellence > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michelle Moore > Sent: Tuesday, July 30, 2013 3:46 PM > To: Jean Wood; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Two Patient Identifiers on slides > > We are writing path #, date of birth & patient initials as identifiers. > Michelle > > > ________________________________ > From: Jean Wood > To: "histonet@lists.utsouthwestern.edu" > > Sent: Tuesday, July 30, 2013 2:37 PM > Subject: [Histonet] Two Patient Identifiers on slides > > > Hello Histonetters, > > Recently we started utilizing a slide labeling component that is > built into our AP Easy LIS system and has accession number, levels and > patients first and last name when labels are printed out. Dymo does not > have a chemically resistant label (we have a Dymo 450 printer) and we have > been putting the labels on AFTER the slides are stained and cover slipped. > > In the meantime, the HT is writing in pencil the accession # and levels on > the slide which is then covered up with the permanent label after cover > slipping. Our Lab Manager is worried that we are not compliant as we do not > have two patient identifiers on throughout the whole process (she wants us > to write patient names on slides in pencil (before staining) and then cover > that up with the pre-printed label after staining. > > 1. What is everyone else doing? > 2. Have any of you found a chemically resistant label compatible with the > Dymo labeler? > > > Jean Wood BS, HT > Fairchild Medical Center Pathology Dept. > Ph:530.841.6243 Fax:530.841.6232 > jwood@fairchildmed.org > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > This message (and any included attachments) is from Rutland Regional > Health Services and is intended only for the addressee(s). The information > contained herein may include privileged or otherwise confidential > information. Unauthorized review, forwarding, printing, copying, > distributing, or using such information is strictly prohibited and may be > unlawful. If you received this message in error, or have reason to believe > you are not authorized to receive it, please promptly delete this message > and notify the sender by e-mail. > > Thank You > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From joewalker <@t> rrmc.org Tue Jul 30 15:59:17 2013 From: joewalker <@t> rrmc.org (Joe W. Walker, Jr.) Date: Tue Jul 30 15:59:27 2013 Subject: [Histonet] Two Patient Identifiers on slides In-Reply-To: References: <87A4EF3F1FBA9D42A7D1EC82F031C0B80A3FD35E07@cliff> <1375213563.32782.YahooMailNeo@web125101.mail.ne1.yahoo.com> <3C2378778400AD448ADA6FD6BDB7CCCC17FFE0FD@RRMBX03.rrmc.local> Message-ID: <3C2378778400AD448ADA6FD6BDB7CCCC17FFE159@RRMBX03.rrmc.local> I would agree that it is not unique but it is acceptable according to the CAP. Joe W. Walker, Jr. MS, SCT(ASCP)CM Anatomical Pathology Manager Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P: 802.747.1790 F: 802.747.6525 Email joewalker@rrmc.org www.rrmc.org Our Vision: To be the Best Community Healthcare System in New England Rutland Regional?Vermont?s 1st Hospital to Achieve Both ANCC Magnet Recognition? and the Governor?s Award for Performance Excellence From: Jay Lundgren [mailto:jaylundgren@gmail.com] Sent: Tuesday, July 30, 2013 4:46 PM To: Joe W. Walker, Jr. Cc: Michelle Moore; Jean Wood; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Two Patient Identifiers on slides Birth date is not a unique patient identifier. Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) On Tue, Jul 30, 2013 at 3:30 PM, Joe W. Walker, Jr. > wrote: I don't think this is technically correct according to the CAP. The question in the most recent checklist states: **REVISED** 07/11/2011 ANP.52000 Specimen ID Phase II The identity of every specimen and image, including blocks, slides, and electron micrographs, is maintained through each step in processing. NOTE: Each block of tissue must be individually labeled with unique patient identifier(s), e.g. accession number etched onto the block or embedded into it. Storage of unlabeled blocks in separate containers that are labeled with patient number or name does not meet this requirement. Evidence of Compliance: ? Written procedure describing system for maintaining specimen identity This question does not state that 2 identifiers must be on the slides and blocks, etc. It states it must be a unique identifier throughout the process. A specimen accession number would technically suffice. You can add additional identifiers if that helps in your process but they are not required. I believe that this is often confused with the General lab question GEN.40491 which requires that any specimen that is collected and submitted to the lab must have 2 unique identifiers on it. Once the specimen is in the lab, the unique accession number would suffice. Now, if you are utilizing the 2 identifiers to help prevent mislabeling, then I think it is a good idea as an extra precaution step. We are fortunate to have a xylene resistant label and printer that allows us to label the slides prior to processing. My two cents as a manager, Joe W. Walker, Jr. MS, SCT(ASCP)CM Anatomical Pathology Manager Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P: 802.747.1790 F: 802.747.6525 Email joewalker@rrmc.org www.rrmc.org Our Vision: To be the Best Community Healthcare System in New England Rutland Regional?Vermont?s 1st Hospital to Achieve Both ANCC Magnet Recognition? and the Governor?s Award for Performance Excellence -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michelle Moore Sent: Tuesday, July 30, 2013 3:46 PM To: Jean Wood; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Two Patient Identifiers on slides We are writing path #, date of birth & patient initials as identifiers. Michelle ________________________________ From: Jean Wood > To: "histonet@lists.utsouthwestern.edu" > Sent: Tuesday, July 30, 2013 2:37 PM Subject: [Histonet] Two Patient Identifiers on slides Hello Histonetters, Recently we started utilizing a slide labeling component that is built into our AP Easy LIS system and has accession number, levels and patients first and last name when labels are printed out. Dymo does not have a chemically resistant label (we have a Dymo 450 printer) and we have been putting the labels on AFTER the slides are stained and cover slipped. In the meantime, the HT is writing in pencil the accession # and levels on the slide which is then covered up with the permanent label after cover slipping. Our Lab Manager is worried that we are not compliant as we do not have two patient identifiers on throughout the whole process (she wants us to write patient names on slides in pencil (before staining) and then cover that up with the pre-printed label after staining. 1. What is everyone else doing? 2. Have any of you found a chemically resistant label compatible with the Dymo labeler? Jean Wood BS, HT Fairchild Medical Center Pathology Dept. Ph:530.841.6243 Fax:530.841.6232 jwood@fairchildmed.org> _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. Thank You _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. Thank You From lhamilton <@t> wcplaboratories.com Tue Jul 30 16:09:25 2013 From: lhamilton <@t> wcplaboratories.com (Lisa Hamilton) Date: Tue Jul 30 16:09:35 2013 Subject: [Histonet] RE: Two Patient Identifiers on slides In-Reply-To: <7EAFE982E328304DA6CE2B677BB76246794ECA94@TN001WEXMBX12.US.chs.net> References: <87A4EF3F1FBA9D42A7D1EC82F031C0B80A3FD35E07@cliff> <7EAFE982E328304DA6CE2B677BB76246794ECA94@TN001WEXMBX12.US.chs.net> Message-ID: <51F82B85.6000900@wcplaboratories.com> The "Two Identifiers" are for Intra-Operative (frozen section) slides only, not routine H&E slides *ANP.11800* .... at least so far! Routine slide information is listed under *ANP.21050 Specimen Identity* -Lisa H. On 7/30/2013 1:41 PM, Boyd, Debbie M wrote: > Your manager is correct. You must have two patient identifiers through the whole process. We write the full name, accession number and designation ( a, b L1 ) on each slide. I have had both CAP and Joint Commission inspectors pull the labels back looking for 2 identifiers. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jean Wood > Sent: Tuesday, July 30, 2013 3:37 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Two Patient Identifiers on slides > > Hello Histonetters, > > Recently we started utilizing a slide labeling component that is built into our AP Easy LIS system and has accession number, levels and patients first and last name when labels are printed out. Dymo does not have a chemically resistant label (we have a Dymo 450 printer) and we have been putting the labels on AFTER the slides are stained and cover slipped. > > In the meantime, the HT is writing in pencil the accession # and levels on the slide which is then covered up with the permanent label after cover slipping. Our Lab Manager is worried that we are not compliant as we do not have two patient identifiers on throughout the whole process (she wants us to write patient names on slides in pencil (before staining) and then cover that up with the pre-printed label after staining. > > 1. What is everyone else doing? > 2. Have any of you found a chemically resistant label compatible with the Dymo labeler? > > > Jean Wood BS, HT > Fairchild Medical Center Pathology Dept. > Ph:530.841.6243 Fax:530.841.6232 > jwood@fairchildmed.org > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -------------------------------------------------------------------------- > Disclaimer: This electronic message may contain information that is > Proprietary, Confidential, or legally privileged or protected. It > is intended only for the use of the individual(s) and entity named > in the message. If you are not an intended recipient of this > message, please notify the sender immediately and delete the > material from your computer. Do not deliver, distribute or copy > this message and do not disclose its contents or take any action in > reliance on the information it contains. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From Vickroy.Jim <@t> mhsil.com Tue Jul 30 17:00:25 2013 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Tue Jul 30 17:00:30 2013 Subject: [Histonet] Background on Jones Stain Message-ID: Recently we switched to a different hydrophilic slide for our special stains and IHC stains. Everything is working great except for one stain......the Jones Silver stain. There is a brown stain all over the slide except where the tissue is. It appears that the Jones has worked. Everyone of our other silver stains work fine with this new slide. We use Ventana stainers and when we use a different hydrophilic slide or even some hydrophobic slides the Jones does not have this background stain all over the slide. Any ideas on what is happening or has anyone else experienced this problem? James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From patrick.lewis <@t> seattlechildrens.org Tue Jul 30 18:13:27 2013 From: patrick.lewis <@t> seattlechildrens.org (Lewis, Patrick) Date: Tue Jul 30 18:13:40 2013 Subject: [Histonet] nanodrop of DNA preps from paraffin sections Message-ID: <3903BE18914F4440834F0E620415FFCA25ACD4E6@PPWEXD01a.childrens.sea.kids> Hi Everyone, Just out of curiosity, what sort of nanodrop values do you typically get with DNA extractions from paraffin sections. I usually get around 100 ng/uL, depending on tissue size, and the number of churls I take. Around 80% are 100 ng to 200 ng/uL. Rarely, I get as high as 1000 ng/uL, 0-5% of the samples I take. Occasionally I get 10-40 ng/uL 5-15% of the samples I take. I primarily extract from tonsil, but I have done several other tissue types. Patrick. I also primarily use QIAgen Mini kits. CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From TMcNemar <@t> lmhealth.org Wed Jul 31 04:48:36 2013 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Wed Jul 31 04:48:28 2013 Subject: [Histonet] RE: Two Patient Identifiers on slides In-Reply-To: <87A4EF3F1FBA9D42A7D1EC82F031C0B80A3FD35E07@cliff> References: <87A4EF3F1FBA9D42A7D1EC82F031C0B80A3FD35E07@cliff> Message-ID: Our cassettes are printed with the accession number, patient name, and a barcode that also contains the DOB. When sectioning, this barcode is scanned at the microtome when the slides are cut. Labels are printed, again at the microtome, and the slides are labeled. The slide label contains the accession number, patient name, level number or special stain, the pathologist's initials, and the hospital address. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jean Wood Sent: Tuesday, July 30, 2013 3:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Two Patient Identifiers on slides Hello Histonetters, Recently we started utilizing a slide labeling component that is built into our AP Easy LIS system and has accession number, levels and patients first and last name when labels are printed out. Dymo does not have a chemically resistant label (we have a Dymo 450 printer) and we have been putting the labels on AFTER the slides are stained and cover slipped. In the meantime, the HT is writing in pencil the accession # and levels on the slide which is then covered up with the permanent label after cover slipping. Our Lab Manager is worried that we are not compliant as we do not have two patient identifiers on throughout the whole process (she wants us to write patient names on slides in pencil (before staining) and then cover that up with the pre-printed label after staining. 1. What is everyone else doing? 2. Have any of you found a chemically resistant label compatible with the Dymo labeler? Jean Wood BS, HT Fairchild Medical Center Pathology Dept. Ph:530.841.6243 Fax:530.841.6232 jwood@fairchildmed.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From mw <@t> personifysearch.com Wed Jul 31 07:05:49 2013 From: mw <@t> personifysearch.com (Matt Ward) Date: Wed Jul 31 07:05:54 2013 Subject: [Histonet] New Job Opportunity - Field Histology Applications Specialist Message-ID: <70e4f1d6e3260c53a3d4f1f871a3041b@mail.gmail.com> Good morning! We have had a new opportunity open with a world leading manufacturer of histology products. Our client is growing and searching for a histology professional to join their team on the West Coast. The ideal candidate will be based in CA, and will work in the field with sales reps as the technical specialist. This opportunity is perfect for someone who is looking to break out of the laboratory and into the field. The opportunity offers a competitive base salary + bonus/commission + great benefits. If this sounds like something you would be interested in learning more about, please contact me at mw@personfiysearch.com, or call 800.875.6188 ext. 103. I look forward to connecting soon! Best Regards, Matt Ward Matt Ward *Account Executive* *Personify* 5020 Weston Parkway Suite 315 Cary NC 27513 (Tel) 800.875.6188 direct ext 103 (Fax) 919.460.0642 www.personifysearch.com From JMaslanka <@t> stpetes.org Wed Jul 31 08:52:33 2013 From: JMaslanka <@t> stpetes.org (JMaslanka@stpetes.org) Date: Wed Jul 31 08:53:21 2013 Subject: [Histonet] ??? Message-ID: Is anyone using Ventana's Vantage software without the Symphony stainer? If so, I would like to ask you a few questions. Thanks in advance Joe Maslanka BS, CT,HT (ASCP) Anatomical Pathology Technical Supervisor St Peter's Hospital,MT 59601 (P)(406) 447-2406 (F)(406)444-2126 Give thanks for ALL things..... "Kindness is the language the blind can see & the deaf can hear- Mark Twain From gu.lang <@t> gmx.at Wed Jul 31 09:13:29 2013 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Wed Jul 31 09:14:56 2013 Subject: AW: [Histonet] nanodrop of DNA preps from paraffin sections In-Reply-To: <3903BE18914F4440834F0E620415FFCA25ACD4E6@PPWEXD01a.childrens.sea.kids> References: <3903BE18914F4440834F0E620415FFCA25ACD4E6@PPWEXD01a.childrens.sea.kids> Message-ID: <000301ce8df8$21baf730$6530e590$@gmx.at> It depends on... We isolate DNA from very small biopsies -- 10-40 ng/?l (10 slices, measured with Qubit; Nanodrop is usuall about 2-3 fold more). >From surgical tissue -- 50-150 ng/?l (1 cm2, 3-4 slices) We use the Qiacube with filter columns for isolation, so too much DNA would be sticking in the silicate-membrane. What is the question behind your question? Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Lewis, Patrick Gesendet: Mittwoch, 31. Juli 2013 01:13 An: 'Histonet@lists.utsouthwestern.edu' Betreff: [Histonet] nanodrop of DNA preps from paraffin sections Hi Everyone, Just out of curiosity, what sort of nanodrop values do you typically get with DNA extractions from paraffin sections. I usually get around 100 ng/uL, depending on tissue size, and the number of churls I take. Around 80% are 100 ng to 200 ng/uL. Rarely, I get as high as 1000 ng/uL, 0-5% of the samples I take. Occasionally I get 10-40 ng/uL 5-15% of the samples I take. I primarily extract from tonsil, but I have done several other tissue types. Patrick. I also primarily use QIAgen Mini kits. CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Pathrm35 <@t> comcast.net Wed Jul 31 10:01:19 2013 From: Pathrm35 <@t> comcast.net (Pathrm35@comcast.net) Date: Wed Jul 31 10:01:44 2013 Subject: [Histonet] polyomavirus antigen immunostain in urine cytology specimens Message-ID: <507690732.2100781.1375282879645.JavaMail.root@sz0123a.westchester.pa.mail.comcast.net> Fellow techs, Does any one have any experience with a immunostain for a polyomavirus antigen in urine cytology specimens? Thanks, Ron From debgranato <@t> yahoo.com Wed Jul 31 10:04:30 2013 From: debgranato <@t> yahoo.com (Debbie Granato) Date: Wed Jul 31 10:04:34 2013 Subject: [Histonet] Shipping Slides Message-ID: <1375283070.53642.YahooMailNeo@web161203.mail.bf1.yahoo.com> Good Morning! ? Can anyone tell me the best way that you have found to ship slides by Fed Ex? ?I need to send several cases out and want the safest way possible to eliminate broken slides. We have tried plastic slide boxes with gauze for cushioning and then taped shut and a few other ways. Are there special transport slide containers, other than the 5 slide holders. Any suggestions would be greatly appreciated! ? Thank you, Debbie Granato HT(ASCP) From benjamin <@t> histologistics.com Wed Jul 31 10:07:21 2013 From: benjamin <@t> histologistics.com (Benjamin) Date: Wed Jul 31 10:07:40 2013 Subject: [Histonet] RE: Two Patient Identifiers on slides In-Reply-To: <7EAFE982E328304DA6CE2B677BB76246794ECA94@TN001WEXMBX12.US.chs.net> References: <87A4EF3F1FBA9D42A7D1EC82F031C0B80A3FD35E07@cliff> <7EAFE982E328304DA6CE2B677BB76246794ECA94@TN001WEXMBX12.US.chs.net> Message-ID: <751B56EE-2745-4A5D-A8A6-99A1E8E14E9A@histologistics.com> I often see blocks from many institutions that only have surgical numbers on them, then after the slides are always expected to have both. Seems like blocks are the only exception to this rule, but it is probably just a commonly practiced noncompliancy type of thing more than an exception. Sent from my iPhone On Jul 30, 2013, at 3:41 PM, "Boyd, Debbie M" wrote: > Your manager is correct. You must have two patient identifiers through the whole process. We write the full name, accession number and designation ( a, b L1 ) on each slide. I have had both CAP and Joint Commission inspectors pull the labels back looking for 2 identifiers. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jean Wood > Sent: Tuesday, July 30, 2013 3:37 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Two Patient Identifiers on slides > > Hello Histonetters, > > Recently we started utilizing a slide labeling component that is built into our AP Easy LIS system and has accession number, levels and patients first and last name when labels are printed out. Dymo does not have a chemically resistant label (we have a Dymo 450 printer) and we have been putting the labels on AFTER the slides are stained and cover slipped. > > In the meantime, the HT is writing in pencil the accession # and levels on the slide which is then covered up with the permanent label after cover slipping. Our Lab Manager is worried that we are not compliant as we do not have two patient identifiers on throughout the whole process (she wants us to write patient names on slides in pencil (before staining) and then cover that up with the pre-printed label after staining. > > 1. What is everyone else doing? > 2. Have any of you found a chemically resistant label compatible with the Dymo labeler? > > > Jean Wood BS, HT > Fairchild Medical Center Pathology Dept. > Ph:530.841.6243 Fax:530.841.6232 > jwood@fairchildmed.org > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -------------------------------------------------------------------------- > Disclaimer: This electronic message may contain information that is > Proprietary, Confidential, or legally privileged or protected. It > is intended only for the use of the individual(s) and entity named > in the message. If you are not an intended recipient of this > message, please notify the sender immediately and delete the > material from your computer. Do not deliver, distribute or copy > this message and do not disclose its contents or take any action in > reliance on the information it contains. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mhale <@t> MiracaLS.com Wed Jul 31 10:10:40 2013 From: mhale <@t> MiracaLS.com (Hale, Meredith) Date: Wed Jul 31 10:10:46 2013 Subject: [Histonet] Louisiana Position Message-ID: <0E828EC51C7CC445A51E53F81B64E8C72DA872@s-irv-exchmb.PathologyPartners.intranet> Great full time opportunity for a Histotechnician in Shreveport, Louisiana ! Regional Urology is looking for a certified HT or HTL to join their laboratory . Candidate must meet the following criteria: * Meet CLIA Grossing Requirements : CFR 493.1489, http://wwwn.cdc.gov/clia/regs/toc.aspx/ ,prior experience grossing GI specimens * HT ASCP Certified Duties include: * Grossing * Cytology preparation * Embedding * Microtomy * Staining * Ability to be flexible and take on additional duties' as needed This is a full time position that offers a competitive rate and flexible hours. Interested applicants should contact Meredith Hale; phone 214-596-2219 or through email mhale@miracals.com Meredith Hale HT (ASCP)cm Director External Sales Support Miraca Life Sciences 6655 North MacArthur Blvd. Irving , Texas 75039 Office: 214-596-2219 Cell: 469-648-8253 Fax: 1-866-688-3280 mhale@miracals.com> From benjamin <@t> histologistics.com Wed Jul 31 10:17:34 2013 From: benjamin <@t> histologistics.com (Benjamin) Date: Wed Jul 31 10:17:43 2013 Subject: [Histonet] Shipping Slides In-Reply-To: <1375283070.53642.YahooMailNeo@web161203.mail.bf1.yahoo.com> References: <1375283070.53642.YahooMailNeo@web161203.mail.bf1.yahoo.com> Message-ID: <757CB77F-5323-4DBA-B402-9984FF674537@histologistics.com> We use a rectangular cut bubble wrap inside the box on top of the slides, then wrap the entire box in bubble wrap a few times around. Its the best skill to teach your interns, cutting bubble wrap, boxing it up and bringing to fedex, then picking up coffee on the way back! Some slide boxes come with bubble wrap from the vendor, we save those and use when shipping also. Sent from my iPhone On Jul 31, 2013, at 11:04 AM, Debbie Granato wrote: > Good Morning! > > Can anyone tell me the best way that you have found to ship slides by Fed Ex? > I need to send several cases out and want the safest way possible to eliminate broken slides. > We have tried plastic slide boxes with gauze for cushioning and then taped shut and a few other ways. Are there special transport slide containers, other than the 5 slide holders. > Any suggestions would be greatly appreciated! > > Thank you, > Debbie Granato HT(ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hhawkins <@t> UTMB.EDU Wed Jul 31 10:28:13 2013 From: hhawkins <@t> UTMB.EDU (Hawkins, Hal K.) Date: Wed Jul 31 10:28:17 2013 Subject: [Histonet] RE: Two Patient Identifiers on slides In-Reply-To: <51F82B85.6000900@wcplaboratories.com> References: <87A4EF3F1FBA9D42A7D1EC82F031C0B80A3FD35E07@cliff> <7EAFE982E328304DA6CE2B677BB76246794ECA94@TN001WEXMBX12.US.chs.net>, <51F82B85.6000900@wcplaboratories.com> Message-ID: <22624908330375439D6382C9F95093FF126B433E@GRMBX1.utmb.edu> I notice the new AP checklists effective 7/31/2013 do not include ANP.11800. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Lisa Hamilton [lhamilton@wcplaboratories.com] Sent: Tuesday, July 30, 2013 4:09 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Two Patient Identifiers on slides The "Two Identifiers" are for Intra-Operative (frozen section) slides only, not routine H&E slides *ANP.11800* .... at least so far! Routine slide information is listed under *ANP.21050 Specimen Identity* -Lisa H. On 7/30/2013 1:41 PM, Boyd, Debbie M wrote: > Your manager is correct. You must have two patient identifiers through the whole process. We write the full name, accession number and designation ( a, b L1 ) on each slide. I have had both CAP and Joint Commission inspectors pull the labels back looking for 2 identifiers. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jean Wood > Sent: Tuesday, July 30, 2013 3:37 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Two Patient Identifiers on slides > > Hello Histonetters, > > Recently we started utilizing a slide labeling component that is built into our AP Easy LIS system and has accession number, levels and patients first and last name when labels are printed out. Dymo does not have a chemically resistant label (we have a Dymo 450 printer) and we have been putting the labels on AFTER the slides are stained and cover slipped. > > In the meantime, the HT is writing in pencil the accession # and levels on the slide which is then covered up with the permanent label after cover slipping. Our Lab Manager is worried that we are not compliant as we do not have two patient identifiers on throughout the whole process (she wants us to write patient names on slides in pencil (before staining) and then cover that up with the pre-printed label after staining. > > 1. What is everyone else doing? > 2. Have any of you found a chemically resistant label compatible with the Dymo labeler? > > > Jean Wood BS, HT > Fairchild Medical Center Pathology Dept. > Ph:530.841.6243 Fax:530.841.6232 > jwood@fairchildmed.org > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -------------------------------------------------------------------------- > Disclaimer: This electronic message may contain information that is > Proprietary, Confidential, or legally privileged or protected. It > is intended only for the use of the individual(s) and entity named > in the message. If you are not an intended recipient of this > message, please notify the sender immediately and delete the > material from your computer. Do not deliver, distribute or copy > this message and do not disclose its contents or take any action in > reliance on the information it contains. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From trathborne <@t> somerset-healthcare.com Wed Jul 31 10:38:09 2013 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Wed Jul 31 10:39:08 2013 Subject: [Histonet] Shipping Slides In-Reply-To: <1375283070.53642.YahooMailNeo@web161203.mail.bf1.yahoo.com> References: <1375283070.53642.YahooMailNeo@web161203.mail.bf1.yahoo.com> Message-ID: <3AD061FE740D464FAC7BF6B5CFB757076C725B93@smcmail02.somerset-healthcare.com> If the slides are well dried, you can also bunch them together and wrap in a paper towel if you don't have too many. Tape when wrapped, then place in a slide box and insert in a padded envelope for shipping. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Debbie Granato Sent: Wednesday, July 31, 2013 11:05 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Shipping Slides Good Morning! ? Can anyone tell me the best way that you have found to ship slides by Fed Ex? ?I need to send several cases out and want the safest way possible to eliminate broken slides. We have tried plastic slide boxes with gauze for cushioning and then taped shut and a few other ways. Are there special transport slide containers, other than the 5 slide holders. Any suggestions would be greatly appreciated! ? Thank you, Debbie Granato HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b427297 <@t> aol.com Wed Jul 31 10:43:51 2013 From: b427297 <@t> aol.com (Jackie O'Connor) Date: Wed Jul 31 10:43:54 2013 Subject: [Histonet] Seeking part time position In-Reply-To: References: <87A4EF3F1FBA9D42A7D1EC82F031C0B80A3FD35E07@cliff> Message-ID: <8D05C1BE3BD74BE-243C-11100@webmail-m277.sysops.aol.com> Posting for a friend who is a highly qualified histotech, looking for a 2-3 day per week permanent position in Northern Illinois. Got a job? Please send me your contact information, or I can provide information to you privately. From epeters2 <@t> gmu.edu Wed Jul 31 11:42:39 2013 From: epeters2 <@t> gmu.edu (Esther C Peters) Date: Wed Jul 31 11:42:47 2013 Subject: [Histonet] Shipping Slides In-Reply-To: <3AD061FE740D464FAC7BF6B5CFB757076C725B93@smcmail02.somerset-healthcare.com> References: <1375283070.53642.YahooMailNeo@web161203.mail.bf1.yahoo.com>, <3AD061FE740D464FAC7BF6B5CFB757076C725B93@smcmail02.somerset-healthcare.com> Message-ID: The trick to successful shipping is to make sure the slides cannot rattle in the slide box. If you put your tissue, gauze, or bubble wrap layer on top and shut the box, shake gently to see if they can move. If so, you can put a piece of tape across all the slides in the box to anchor them in place (stick the tape ends to the outside of the box. Then make sure the box is securely shut, putting tape or rubber bands around the outside in both directions. Then pack in a well-padded sturdy shipping box! Esther C. Peters, Ph.D. Assistant Professor Environmental Science & Policy George Mason University ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Rathborne, Toni [trathborne@somerset-healthcare.com] Sent: Wednesday, July 31, 2013 11:38 AM To: 'Debbie Granato'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Shipping Slides If the slides are well dried, you can also bunch them together and wrap in a paper towel if you don't have too many. Tape when wrapped, then place in a slide box and insert in a padded envelope for shipping. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Debbie Granato Sent: Wednesday, July 31, 2013 11:05 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Shipping Slides Good Morning! Can anyone tell me the best way that you have found to ship slides by Fed Ex? I need to send several cases out and want the safest way possible to eliminate broken slides. We have tried plastic slide boxes with gauze for cushioning and then taped shut and a few other ways. Are there special transport slide containers, other than the 5 slide holders. Any suggestions would be greatly appreciated! Thank you, Debbie Granato HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hymclab.hymclab <@t> ministryhealth.org Wed Jul 31 11:46:29 2013 From: hymclab.hymclab <@t> ministryhealth.org (hymclab) Date: Wed Jul 31 11:46:43 2013 Subject: [Histonet] Two Patient Identifiers on slides In-Reply-To: <3C2378778400AD448ADA6FD6BDB7CCCC17FFE0FD@RRMBX03.rrmc.local> References: <87A4EF3F1FBA9D42A7D1EC82F031C0B80A3FD35E07@cliff> <1375213563.32782.YahooMailNeo@web125101.mail.ne1.yahoo.com> <3C2378778400AD448ADA6FD6BDB7CCCC17FFE0FD@RRMBX03.rrmc.local> Message-ID: The below statement is correct and our last Joint Commission inpector clarified that the 2 patient identifier rule only applies to the specimen containers coming in the door. Once they are accessioned in Path you can label slides and blocks with only the accession number and do not need the 2 patient identifiers on them. We do make sure the patient name is on the side of the block if we send out just for completeness sakr and all of out final slides labels have the patient name on them as well. Dawn D. Schneider, HT(ASCP) Lead HT Howard Young Medical Center 240 Maple Ave. Woodruff, WI 54558 715-356-8174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe W. Walker, Jr. Sent: Tuesday, July 30, 2013 3:31 PM To: Michelle Moore; Jean Wood; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Two Patient Identifiers on slides I don't think this is technically correct according to the CAP. The question in the most recent checklist states: **REVISED** 07/11/2011 ANP.52000 Specimen ID Phase II The identity of every specimen and image, including blocks, slides, and electron micrographs, is maintained through each step in processing. NOTE: Each block of tissue must be individually labeled with unique patient identifier(s), e.g. accession number etched onto the block or embedded into it. Storage of unlabeled blocks in separate containers that are labeled with patient number or name does not meet this requirement. Evidence of Compliance: ? Written procedure describing system for maintaining specimen identity This question does not state that 2 identifiers must be on the slides and blocks, etc. It states it must be a unique identifier throughout the process. A specimen accession number would technically suffice. You can add additional identifiers if that helps in your process but they are not required. I believe that this is often confused with the General lab question GEN.40491 which requires that any specimen that is collected and submitted to the lab must have 2 unique identifiers on it. Once the specimen is in the lab, the unique accession number would suffice. Now, if you are utilizing the 2 identifiers to help prevent mislabeling, then I think it is a good idea as an extra precaution step. We are fortunate to have a xylene resistant label and printer that allows us to label the slides prior to processing. My two cents as a manager, Joe W. Walker, Jr. MS, SCT(ASCP)CM Anatomical Pathology Manager Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P: 802.747.1790 F: 802.747.6525 Email joewalker@rrmc.org www.rrmc.org Our Vision: To be the Best Community Healthcare System in New England Rutland Regional?Vermont?s 1st Hospital to Achieve Both ANCC Magnet Recognition? and the Governor?s Award for Performance Excellence -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michelle Moore Sent: Tuesday, July 30, 2013 3:46 PM To: Jean Wood; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Two Patient Identifiers on slides We are writing path #, date of birth & patient initials as identifiers. Michelle ________________________________ From: Jean Wood To: "histonet@lists.utsouthwestern.edu" Sent: Tuesday, July 30, 2013 2:37 PM Subject: [Histonet] Two Patient Identifiers on slides Hello Histonetters, Recently we started utilizing a slide labeling component that is built into our AP Easy LIS system and has accession number, levels and patients first and last name when labels are printed out. Dymo does not have a chemically resistant label (we have a Dymo 450 printer) and we have been putting the labels on AFTER the slides are stained and cover slipped. In the meantime, the HT is writing in pencil the accession # and levels on the slide which is then covered up with the permanent label after cover slipping. Our Lab Manager is worried that we are not compliant as we do not have two patient identifiers on throughout the whole process (she wants us to write patient names on slides in pencil (before staining) and then cover that up with the pre-printed label after staining. 1. What is everyone else doing? 2. Have any of you found a chemically resistant label compatible with the Dymo labeler? Jean Wood BS, HT Fairchild Medical Center Pathology Dept. Ph:530.841.6243 Fax:530.841.6232 jwood@fairchildmed.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. Thank You CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. From jaylundgren <@t> gmail.com Wed Jul 31 12:41:59 2013 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Wed Jul 31 12:42:03 2013 Subject: [Histonet] Background on Jones Stain In-Reply-To: References: Message-ID: I'd guess it's silver residue. For some reason, non-specific silver is sticking to the new slides. Why don't you just buy a case of your old slides to keep around for the Jones stain? Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) On Tue, Jul 30, 2013 at 5:00 PM, Vickroy, Jim wrote: > > Recently we switched to a different hydrophilic slide for our special > stains and IHC stains. Everything is working great except for one > stain......the Jones Silver stain. There is a brown stain all over the > slide except where the tissue is. It appears that the Jones has worked. > Everyone of our other silver stains work fine with this new slide. We > use Ventana stainers and when we use a different hydrophilic slide or even > some hydrophobic slides the Jones does not have this background stain all > over the slide. Any ideas on what is happening or has anyone else > experienced this problem? > > James Vickroy BS, HT(ASCP) > > Surgical and Autopsy Pathology Technical Supervisor > Memorial Medical Center > 217-788-4046 > > > ________________________________ > This message (including any attachments) contains confidential information > intended for a specific individual and purpose, and is protected by law. If > you are not the intended recipient, you should delete this message. Any > disclosure, copying, or distribution of this message, or the taking of any > action based on it, is strictly prohibited. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Caroline.Pratt <@t> uphs.upenn.edu Wed Jul 31 12:46:34 2013 From: Caroline.Pratt <@t> uphs.upenn.edu (Pratt, Caroline) Date: Wed Jul 31 12:46:42 2013 Subject: [Histonet] RE: Two Patient Identifiers on slides In-Reply-To: References: <87A4EF3F1FBA9D42A7D1EC82F031C0B80A3FD35E07@cliff> Message-ID: Just for clarification, patient first and last name? Thanks! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Wednesday, July 31, 2013 5:49 AM To: 'Jean Wood'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Two Patient Identifiers on slides Our cassettes are printed with the accession number, patient name, and a barcode that also contains the DOB. When sectioning, this barcode is scanned at the microtome when the slides are cut. Labels are printed, again at the microtome, and the slides are labeled. The slide label contains the accession number, patient name, level number or special stain, the pathologist's initials, and the hospital address. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jean Wood Sent: Tuesday, July 30, 2013 3:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Two Patient Identifiers on slides Hello Histonetters, Recently we started utilizing a slide labeling component that is built into our AP Easy LIS system and has accession number, levels and patients first and last name when labels are printed out. Dymo does not have a chemically resistant label (we have a Dymo 450 printer) and we have been putting the labels on AFTER the slides are stained and cover slipped. In the meantime, the HT is writing in pencil the accession # and levels on the slide which is then covered up with the permanent label after cover slipping. Our Lab Manager is worried that we are not compliant as we do not have two patient identifiers on throughout the whole process (she wants us to write patient names on slides in pencil (before staining) and then cover that up with the pre-printed label after staining. 1. What is everyone else doing? 2. Have any of you found a chemically resistant label compatible with the Dymo labeler? Jean Wood BS, HT Fairchild Medical Center Pathology Dept. Ph:530.841.6243 Fax:530.841.6232 jwood@fairchildmed.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From Sherrian.McAnn <@t> va.gov Wed Jul 31 12:52:00 2013 From: Sherrian.McAnn <@t> va.gov (McAnn, Sherrian) Date: Wed Jul 31 12:52:53 2013 Subject: [Histonet] Recycled Xylene Message-ID: <61E2B58CECEF384094A363989D47C0900A0A82DD@VHAV17MSGA2.v17.med.va.gov> We do our own recycling of alcohol and xylene. W e use the recycled xylene for everything including cleaning the processors.. No problems..been using it for years -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Monday, July 29, 2013 10:47 AM To: Histonet Post (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Recycled Xylene Does anyone out there have any problems with using recycled xylene to clean the tissue processor? I was just told that we should not use recycled xylene to clean the tissue processor. How about using recycled xylene for either processing or staining? Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Caroline.Pratt <@t> uphs.upenn.edu Wed Jul 31 13:13:36 2013 From: Caroline.Pratt <@t> uphs.upenn.edu (Pratt, Caroline) Date: Wed Jul 31 13:13:52 2013 Subject: [Histonet] Two Patient Identifiers on slides In-Reply-To: <3C2378778400AD448ADA6FD6BDB7CCCC17FFE0FD@RRMBX03.rrmc.local> References: <87A4EF3F1FBA9D42A7D1EC82F031C0B80A3FD35E07@cliff><1375213563.32782.YahooMailNeo@web125101.mail.ne1.yahoo.com> <3C2378778400AD448ADA6FD6BDB7CCCC17FFE0FD@RRMBX03.rrmc.local> Message-ID: My understanding was the same as Joe's and at least to date, that is what we have presented during survey and it has been deemed acceptable. However, we are accredited by TJC not CAP. My question to those of you who are providing 2 patient identifiers on blocks? How do you make it fit? If anyone could scan an image to Caroline.Pratt@uphs.upenn.edu I would greatly appreciate it! As of now, we have the unique patient accession number and a barcode with the accession number as well. We are in our survey window I would like to be sure we are compliant. One more question, if you label slides and blocks with a name and DOB from the requisition and find out during insurance validation that the name is misspelled or the DOB is inaccurate, how do you handle that? Thanks for your help. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe W. Walker, Jr. Sent: Tuesday, July 30, 2013 4:31 PM To: Michelle Moore; Jean Wood; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Two Patient Identifiers on slides I don't think this is technically correct according to the CAP. The question in the most recent checklist states: **REVISED** 07/11/2011 ANP.52000 Specimen ID Phase II The identity of every specimen and image, including blocks, slides, and electron micrographs, is maintained through each step in processing. NOTE: Each block of tissue must be individually labeled with unique patient identifier(s), e.g. accession number etched onto the block or embedded into it. Storage of unlabeled blocks in separate containers that are labeled with patient number or name does not meet this requirement. Evidence of Compliance: ? Written procedure describing system for maintaining specimen identity This question does not state that 2 identifiers must be on the slides and blocks, etc. It states it must be a unique identifier throughout the process. A specimen accession number would technically suffice. You can add additional identifiers if that helps in your process but they are not required. I believe that this is often confused with the General lab question GEN.40491 which requires that any specimen that is collected and submitted to the lab must have 2 unique identifiers on it. Once the specimen is in the lab, the unique accession number would suffice. Now, if you are utilizing the 2 identifiers to help prevent mislabeling, then I think it is a good idea as an extra precaution step. We are fortunate to have a xylene resistant label and printer that allows us to label the slides prior to processing. My two cents as a manager, Joe W. Walker, Jr. MS, SCT(ASCP)CM Anatomical Pathology Manager Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P: 802.747.1790 F: 802.747.6525 Email joewalker@rrmc.org www.rrmc.org Our Vision: To be the Best Community Healthcare System in New England Rutland Regional?Vermont?s 1st Hospital to Achieve Both ANCC Magnet Recognition? and the Governor?s Award for Performance Excellence -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michelle Moore Sent: Tuesday, July 30, 2013 3:46 PM To: Jean Wood; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Two Patient Identifiers on slides We are writing path #, date of birth & patient initials as identifiers. Michelle ________________________________ From: Jean Wood To: "histonet@lists.utsouthwestern.edu" Sent: Tuesday, July 30, 2013 2:37 PM Subject: [Histonet] Two Patient Identifiers on slides Hello Histonetters, Recently we started utilizing a slide labeling component that is built into our AP Easy LIS system and has accession number, levels and patients first and last name when labels are printed out. Dymo does not have a chemically resistant label (we have a Dymo 450 printer) and we have been putting the labels on AFTER the slides are stained and cover slipped. In the meantime, the HT is writing in pencil the accession # and levels on the slide which is then covered up with the permanent label after cover slipping. Our Lab Manager is worried that we are not compliant as we do not have two patient identifiers on throughout the whole process (she wants us to write patient names on slides in pencil (before staining) and then cover that up with the pre-printed label after staining. 1. What is everyone else doing? 2. Have any of you found a chemically resistant label compatible with the Dymo labeler? Jean Wood BS, HT Fairchild Medical Center Pathology Dept. Ph:530.841.6243 Fax:530.841.6232 jwood@fairchildmed.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. Thank You The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From PAMarcum <@t> uams.edu Wed Jul 31 13:19:10 2013 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Wed Jul 31 13:19:20 2013 Subject: [Histonet] RE: Two Patient Identifiers on slides In-Reply-To: References: <87A4EF3F1FBA9D42A7D1EC82F031C0B80A3FD35E07@cliff> Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA32CA175EEA@Mail2Node2.ad.uams.edu> We use the surgical case number and a minimum of the first 5 letters of the patients name on barcode labeled cassettes and then that code will be transferred to the slides as they are printed. We have found that having two identifiers on every cassette and slide has saved us when an error was made with the cassette labeling or slide labeling process. It has been a way to confirm or clear up an issue very quickly. Whether it is required or not; at this point we will continue to use both identifiers for both the patient's safety and our ability to backtrack/clear issues of possible miss identification of a cassette or slide. We are all human and mistakes happen so plan a way to limit the possibilities. Pam Marcum UAMS -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pratt, Caroline Sent: Wednesday, July 31, 2013 12:47 PM To: Tom McNemar; Jean Wood; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Two Patient Identifiers on slides Just for clarification, patient first and last name? Thanks! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Wednesday, July 31, 2013 5:49 AM To: 'Jean Wood'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Two Patient Identifiers on slides Our cassettes are printed with the accession number, patient name, and a barcode that also contains the DOB. When sectioning, this barcode is scanned at the microtome when the slides are cut. Labels are printed, again at the microtome, and the slides are labeled. The slide label contains the accession number, patient name, level number or special stain, the pathologist's initials, and the hospital address. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jean Wood Sent: Tuesday, July 30, 2013 3:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Two Patient Identifiers on slides Hello Histonetters, Recently we started utilizing a slide labeling component that is built into our AP Easy LIS system and has accession number, levels and patients first and last name when labels are printed out. Dymo does not have a chemically resistant label (we have a Dymo 450 printer) and we have been putting the labels on AFTER the slides are stained and cover slipped. In the meantime, the HT is writing in pencil the accession # and levels on the slide which is then covered up with the permanent label after cover slipping. Our Lab Manager is worried that we are not compliant as we do not have two patient identifiers on throughout the whole process (she wants us to write patient names on slides in pencil (before staining) and then cover that up with the pre-printed label after staining. 1. What is everyone else doing? 2. Have any of you found a chemically resistant label compatible with the Dymo labeler? Jean Wood BS, HT Fairchild Medical Center Pathology Dept. Ph:530.841.6243 Fax:530.841.6232 jwood@fairchildmed.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PAMarcum <@t> uams.edu Wed Jul 31 13:35:37 2013 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Wed Jul 31 13:35:43 2013 Subject: [Histonet] Two Patient Identifiers on slides In-Reply-To: References: <87A4EF3F1FBA9D42A7D1EC82F031C0B80A3FD35E07@cliff><1375213563.32782.YahooMailNeo@web125101.mail.ne1.yahoo.com> <3C2378778400AD448ADA6FD6BDB7CCCC17FFE0FD@RRMBX03.rrmc.local> Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA32CA175F12@Mail2Node2.ad.uams.edu> Due to legal issues, if we have a cassette mislabeled we request a cassette with the correct patient information immediately and re-embed the block. Biopsies are a concern as it is sometimes difficult to re-embed a small tissue, so we do our best. We have had times when we cross out the barcode as we could not reliably re-embed and carefully make notes for any future legal problems (no matter how unlikely the case may be) to establish the identity clearly. If the slides were cut and we cannot go back to the block due to tissue size or disease state we use the same procedure as cassettes and a paper label. The real issue is training everyone to double check the name and case number with the paperwork as the cassettes and slides are printed and have no mistakes. We are human and has been proven difficult at times. Pam Marcum -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pratt, Caroline Sent: Wednesday, July 31, 2013 1:14 PM To: Joe W. Walker, Jr.; Michelle Moore; Jean Wood; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Two Patient Identifiers on slides My understanding was the same as Joe's and at least to date, that is what we have presented during survey and it has been deemed acceptable. However, we are accredited by TJC not CAP. My question to those of you who are providing 2 patient identifiers on blocks? How do you make it fit? If anyone could scan an image to Caroline.Pratt@uphs.upenn.edu I would greatly appreciate it! As of now, we have the unique patient accession number and a barcode with the accession number as well. We are in our survey window I would like to be sure we are compliant. One more question, if you label slides and blocks with a name and DOB from the requisition and find out during insurance validation that the name is misspelled or the DOB is inaccurate, how do you handle that? Thanks for your help. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe W. Walker, Jr. Sent: Tuesday, July 30, 2013 4:31 PM To: Michelle Moore; Jean Wood; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Two Patient Identifiers on slides I don't think this is technically correct according to the CAP. The question in the most recent checklist states: **REVISED** 07/11/2011 ANP.52000 Specimen ID Phase II The identity of every specimen and image, including blocks, slides, and electron micrographs, is maintained through each step in processing. NOTE: Each block of tissue must be individually labeled with unique patient identifier(s), e.g. accession number etched onto the block or embedded into it. Storage of unlabeled blocks in separate containers that are labeled with patient number or name does not meet this requirement. Evidence of Compliance: ? Written procedure describing system for maintaining specimen identity This question does not state that 2 identifiers must be on the slides and blocks, etc. It states it must be a unique identifier throughout the process. A specimen accession number would technically suffice. You can add additional identifiers if that helps in your process but they are not required. I believe that this is often confused with the General lab question GEN.40491 which requires that any specimen that is collected and submitted to the lab must have 2 unique identifiers on it. Once the specimen is in the lab, the unique accession number would suffice. Now, if you are utilizing the 2 identifiers to help prevent mislabeling, then I think it is a good idea as an extra precaution step. We are fortunate to have a xylene resistant label and printer that allows us to label the slides prior to processing. My two cents as a manager, Joe W. Walker, Jr. MS, SCT(ASCP)CM Anatomical Pathology Manager Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P: 802.747.1790 F: 802.747.6525 Email joewalker@rrmc.org www.rrmc.org Our Vision: To be the Best Community Healthcare System in New England Rutland Regional?Vermont?s 1st Hospital to Achieve Both ANCC Magnet Recognition? and the Governor?s Award for Performance Excellence -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michelle Moore Sent: Tuesday, July 30, 2013 3:46 PM To: Jean Wood; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Two Patient Identifiers on slides We are writing path #, date of birth & patient initials as identifiers. Michelle ________________________________ From: Jean Wood To: "histonet@lists.utsouthwestern.edu" Sent: Tuesday, July 30, 2013 2:37 PM Subject: [Histonet] Two Patient Identifiers on slides Hello Histonetters, Recently we started utilizing a slide labeling component that is built into our AP Easy LIS system and has accession number, levels and patients first and last name when labels are printed out. Dymo does not have a chemically resistant label (we have a Dymo 450 printer) and we have been putting the labels on AFTER the slides are stained and cover slipped. In the meantime, the HT is writing in pencil the accession # and levels on the slide which is then covered up with the permanent label after cover slipping. Our Lab Manager is worried that we are not compliant as we do not have two patient identifiers on throughout the whole process (she wants us to write patient names on slides in pencil (before staining) and then cover that up with the pre-printed label after staining. 1. What is everyone else doing? 2. Have any of you found a chemically resistant label compatible with the Dymo labeler? Jean Wood BS, HT Fairchild Medical Center Pathology Dept. Ph:530.841.6243 Fax:530.841.6232 jwood@fairchildmed.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. Thank You The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From b-frederick <@t> northwestern.edu Wed Jul 31 14:14:13 2013 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Wed Jul 31 14:14:21 2013 Subject: [Histonet] Shipping Slides In-Reply-To: <757CB77F-5323-4DBA-B402-9984FF674537@histologistics.com> References: <1375283070.53642.YahooMailNeo@web161203.mail.bf1.yahoo.com> <757CB77F-5323-4DBA-B402-9984FF674537@histologistics.com> Message-ID: <62C639732D3F274DACED033EBDF6ADAF20D57B1A@evcspmbx2.ads.northwestern.edu> Same here and then in a box with more bubble wrap or we use some of those disposable bench underpads for space filler..... Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Benjamin Sent: Wednesday, July 31, 2013 10:18 AM To: Debbie Granato Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Shipping Slides We use a rectangular cut bubble wrap inside the box on top of the slides, then wrap the entire box in bubble wrap a few times around. Its the best skill to teach your interns, cutting bubble wrap, boxing it up and bringing to fedex, then picking up coffee on the way back! Some slide boxes come with bubble wrap from the vendor, we save those and use when shipping also. Sent from my iPhone On Jul 31, 2013, at 11:04 AM, Debbie Granato wrote: > Good Morning! > > Can anyone tell me the best way that you have found to ship slides by Fed Ex? > I need to send several cases out and want the safest way possible to eliminate broken slides. > We have tried plastic slide boxes with gauze for cushioning and then taped shut and a few other ways. Are there special transport slide containers, other than the 5 slide holders. > Any suggestions would be greatly appreciated! > > Thank you, > Debbie Granato HT(ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sforeman <@t> labpath.com Wed Jul 31 14:47:00 2013 From: sforeman <@t> labpath.com (Susan Foreman) Date: Wed Jul 31 14:51:09 2013 Subject: [Histonet] Collagen IV FITC Message-ID: <00a701ce8e26$b8e3ee90$2aabcbb0$@com> Is anyone out there running Collagen IV by FITC? Derm cases? Which vendor are you most happy with? Any advice on this antibody would be really appreciated? Many Thanks, Susan From rsrichmond <@t> gmail.com Wed Jul 31 18:07:15 2013 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Wed Jul 31 18:07:24 2013 Subject: [Histonet] Re: Two Patient Identifiers on slides Message-ID: Realizing we're talking about regulator compliance rather than good sense here - nonetheless let a pathologist observe that having the patient's name on the slide is a very important defense against mixing up cases at sign-out. Small labs still usually have no identifier on the slide other than a hand-scribbled accession number (two of the three small labs I'm working in at the moment don't - both CAP accredited), and it's easy for the pathologist to misread the label. I saw a mixup like this just a few days ago - it wasn't my case, but I should have caught the misreading when I reviewed the case, which involved a squamous carcinoma that wasn't. Good management, bad medicine. Bob Richmond Samurai Pathologist Maryville TN From b427297 <@t> aol.com Wed Jul 31 19:09:03 2013 From: b427297 <@t> aol.com (Jackie O'Connor) Date: Wed Jul 31 19:09:09 2013 Subject: [Histonet] Position in N. Illinois In-Reply-To: References: Message-ID: <8D05C6277461D6E-2754-13397@webmail-m129.sysops.aol.com> Posting for a friend looking for a permanent 2-3 day a week position in N. Illinois. Very qualified histotech. Please contact me privately. Jackie O'