From chirayu2011 <@t> gmail.com Tue Jan 1 16:17:45 2013 From: chirayu2011 <@t> gmail.com (Chirayu Sharma) Date: Tue Jan 1 16:17:51 2013 Subject: [Histonet] Regarding a problem in manual paraffin embedding Message-ID: Hello Histonetters I am having a problem in Manual Paraffin Embedding of Embryonic Day 16-18 mice heads. My protocol for Paraffin embedding is Fix in 4%NBF for 2 days followed by rinsing the whole head of mice in running water for overnight. Series of ethanol wash 50% -4 hrs 70% 4 hrs 96% 4 hrs 100% 4 hrs Xylene-5 hours Series of Paraffin wash each consisting of 30 minutes interval till the smell of xylene is not there in the Paraffin bath. Finally prepare the block. My problem is even after putting in Xylene for 5 hours I didn?t see clearing of mice heads. I tried with one spare mice heads and kept in xylene for 8 hours but I did not see clearing too. Later I put the same head still without clearing in Parafin and prepare the block, I found the head to be soft and the initial part of the head facing the microtome is white. And flecks of wax could be removed easily from the top of tissue. Another problem is even these tissues are able to cut in some instances the sections tear when putting in water bath and they look something whitish appearance when placed in slides. My problem is I cannot remove the brain and skin for penetration of xylene as my study area does not allow me. It would be so nice if somebody would share me their Manual Paraffin Embedding protocol of whole head of mice in embryonic day 17-18 and Postnatal day 0 and 1 so that I can get rid of these problems. Chirayu From max_histo_00 <@t> yahoo.it Tue Jan 1 18:15:52 2013 From: max_histo_00 <@t> yahoo.it (Massimo) Date: Tue Jan 1 18:15:58 2013 Subject: [Histonet] Regarding a problem in manual paraffin embedding In-Reply-To: References: Message-ID: <1357085752.79728.YahooMailNeo@web172006.mail.ir2.yahoo.com> Hi, I think that your problem is not an enough infiltration time into Xylene but It is a not complete? dehydration of the specimen quite thick. When it happens I come back to new absolute ethanol and then again into Xylene up to complete clearification. I process small pieces so my scheduled times in the procedure are not suitable for your thick specimen. It is not advisable a long time in Xylene because it makes the piece fragile on cutting operation. It is better to process them?for a longer time into 95? and/or 100? ethanol. Preferably in 95? ethanol because the 100? one makes the pieces too hard. I use an intermediate mix of 50% 95? and 50% 100? ethanol solution too. It is advisable to make two or more steps in new ethanol and Xylene solutions. Kind Regards and wishing for a Happy New Year Massimo Tosi "A humble Chemical Engineer who loves Histology" ________________________________ Da: Chirayu Sharma A: histonet@lists.utsouthwestern.edu Inviato: Marted? 1 Gennaio 2013 23:17 Oggetto: [Histonet] Regarding a problem in manual paraffin embedding Hello Histonetters I am having a problem in Manual Paraffin Embedding of Embryonic Day 16-18 mice heads. My protocol for Paraffin embedding is Fix in 4%NBF for 2 days followed by rinsing the whole head of mice in running water for overnight. Series of ethanol wash 50% -4 hrs 70% 4 hrs 96% 4 hrs 100% 4 hrs Xylene-5 hours Series of Paraffin wash each consisting of 30 minutes interval till the smell of xylene is not there in the Paraffin bath. Finally prepare the block. My problem is even after putting in Xylene for 5 hours I didn?t see clearing of mice heads. I tried with one spare mice heads and kept in xylene for 8 hours but I did not see clearing too. Later I put the same head still without clearing in Parafin and prepare the block, I found the head to be soft and the initial part of the head facing the microtome is white. And flecks of wax could be removed easily from the top of tissue. Another problem is even these tissues are able to cut in some instances the sections tear when putting? in water bath and they look something whitish appearance when placed in slides. My problem is I cannot remove the brain and skin for penetration of xylene as my study area does not allow me. It would be so nice if somebody would share me their Manual Paraffin Embedding protocol of whole head of mice in embryonic day 17-18 and Postnatal day 0 and 1 so that I can get rid of these problems. Chirayu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Barry.R.Rittman <@t> uth.tmc.edu Wed Jan 2 05:31:58 2013 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Wed Jan 2 05:31:27 2013 Subject: [Histonet] Regarding a problem in manual paraffin embedding In-Reply-To: References: Message-ID: <12A4DAFC2FEBB84B8DED5F5E9201B4E917C39FDE3E@UTHCMS1.uthouston.edu> Chirayu a happy new year to you. I am guessing that there could be 3 possible problems. 1. It appears that the xylene is not changed - maybe the xylene should have more than one change to eliminate all the alcohol. 2. The alcohol you are using as 100% may have some traces of water in it. 3. There may be small traces of bone in the embryos and if so these will appear whitish and cause problems in cutting and so on. May need to eliminate this with a demineralizaton step before dehydration. Hope that these suggestion are helpful. Barry From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Chirayu Sharma [chirayu2011@gmail.com] Sent: Tuesday, January 01, 2013 4:17 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Regarding a problem in manual paraffin embedding Hello Histonetters I am having a problem in Manual Paraffin Embedding of Embryonic Day 16-18 mice heads. My protocol for Paraffin embedding is Fix in 4%NBF for 2 days followed by rinsing the whole head of mice in running water for overnight. Series of ethanol wash 50% -4 hrs 70% 4 hrs 96% 4 hrs 100% 4 hrs Xylene-5 hours Series of Paraffin wash each consisting of 30 minutes interval till the smell of xylene is not there in the Paraffin bath. Finally prepare the block. My problem is even after putting in Xylene for 5 hours I didn?t see clearing of mice heads. I tried with one spare mice heads and kept in xylene for 8 hours but I did not see clearing too. Later I put the same head still without clearing in Parafin and prepare the block, I found the head to be soft and the initial part of the head facing the microtome is white. And flecks of wax could be removed easily from the top of tissue. Another problem is even these tissues are able to cut in some instances the sections tear when putting in water bath and they look something whitish appearance when placed in slides. My problem is I cannot remove the brain and skin for penetration of xylene as my study area does not allow me. It would be so nice if somebody would share me their Manual Paraffin Embedding protocol of whole head of mice in embryonic day 17-18 and Postnatal day 0 and 1 so that I can get rid of these problems. Chirayu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Jan 2 08:36:29 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jan 2 08:36:37 2013 Subject: [Histonet] Regarding a problem in manual paraffin embedding In-Reply-To: References: Message-ID: <1357137389.78244.YahooMailNeo@web163103.mail.bf1.yahoo.com> Chirayu: I think there are several issues in your protocol: 1- the fixation time looks well but when you wash the fixed specimen overnight with running water you "unfix" the tissue because formalin fixation is totally reversible. You end with an unfixed tissue. After fixation do not wash the fixed tissues and start the dehydration directly. 2- the graduated dehydration looks well but you should double the dehydration steps times. 3- you complain that the heads do not "clear" and they will never clear. It is a common misconception that xylene is a "clearant" and it is not. Xylene is a paraffin wax solvent. The term "clearant" is misused in this case and refers to some chemicals, like?aniline oil?and especially cedar-wood oil that have the ability to make dehydrated tissues transparent but?are no good paraffin solvents and that is why after cedar-wood oil some paraffin solvent, like benzene and xylene, has to be used. If a tissue was made transparent by the aniline oil or the cedar-wood oil, it remained transparent?after being immersed in benzene or xylene. Xylene by itself will NOT make tissues transparent. So do not worry about that, what you need is to increase the time in xylene?using at least 3 changes of?3 hours each. 4- the description of the sections separating in contact with the water is a sign of incomplete infiltration (too little time in xylene and probably in paraffin also) as is the whitish appearance of tissue when sectioning. Summing up, you should eliminate the water washing, duplicate the dehydration times and have 3 xylene changes?of 3 hours each (=9 hours). Finally you do not wash the tissue in paraffin; the tissues have to remain in the paraffin. Try using 4 paraffin infiltration periods of at least 2 hours each. Ren? J. From: Chirayu Sharma To: histonet@lists.utsouthwestern.edu Sent: Tuesday, January 1, 2013 5:17 PM Subject: [Histonet] Regarding a problem in manual paraffin embedding Hello Histonetters I am having a problem in Manual Paraffin Embedding of Embryonic Day 16-18 mice heads. My protocol for Paraffin embedding is Fix in 4%NBF for 2 days followed by rinsing the whole head of mice in running water for overnight. Series of ethanol wash 50% -4 hrs 70% 4 hrs 96% 4 hrs 100% 4 hrs Xylene-5 hours Series of Paraffin wash each consisting of 30 minutes interval till the smell of xylene is not there in the Paraffin bath. Finally prepare the block. My problem is even after putting in Xylene for 5 hours I didn?t see clearing of mice heads. I tried with one spare mice heads and kept in xylene for 8 hours but I did not see clearing too. Later I put the same head still without clearing in Parafin and prepare the block, I found the head to be soft and the initial part of the head facing the microtome is white. And flecks of wax could be removed easily from the top of tissue. Another problem is even these tissues are able to cut in some instances the sections tear when putting? in water bath and they look something whitish appearance when placed in slides. My problem is I cannot remove the brain and skin for penetration of xylene as my study area does not allow me. It would be so nice if somebody would share me their Manual Paraffin Embedding protocol of whole head of mice in embryonic day 17-18 and Postnatal day 0 and 1 so that I can get rid of these problems. Chirayu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TJohnson <@t> gnf.org Wed Jan 2 10:30:41 2013 From: TJohnson <@t> gnf.org (Teri Johnson) Date: Wed Jan 2 10:30:46 2013 Subject: [Histonet] Melanin Bleaching Message-ID: <9F3CFEE76E51B64991C7485270890B402E6F124A@EX5.lj.gnf.org> Hi Janci, We have used 10% H2O2 overnight and 3% at 55 degrees for 2 hours prior to immunostaining and both worked adequately to bleach the melanin. However, we did have trouble with tissue falling off at 2 hours, so you might need to test it in your own lab and see if you can get away with less time and still get adequate bleaching. We also have successfully used Azure B staining for turning the melanin green. It might be a better alternative; you can just use it as a counterstain instead of hematoxylin as it also stains the nuclei. Let me know and I can send you a protocol if you need it. Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 From AGleiberman <@t> cbiolabs.com Wed Jan 2 11:04:09 2013 From: AGleiberman <@t> cbiolabs.com (Anatoli Gleiberman) Date: Wed Jan 2 11:04:48 2013 Subject: [Histonet] Regarding a problem in manual paraffin embedding In-Reply-To: References: Message-ID: <77BC2EEB6AC66C49AEF794DC98BE314C012312C1BA@cbiolabs05.CBiolabs.local> Chirayu, Usual problems with heads of late mouse embryos is poor dehydrations. My recommendation is to use isopropanol instead of absolute ethanol. My protocol that worked very well for many years was: Fixation with NBF for 4-6h, short rinse with PBS and storage at 70% ethanol overnight (Alternative fixation - 6 parts of absolute ethanol, 3 parts of distilled water, 1 part of 37% formaldehyde - 4-6 h, transfer in 70% ethanol overnight) 4 changes of isopropanol, 1h each 3 changes of xylene - 45 min each 3 changes of paraffin 1h each. Anatoli Gleiberman, PhD Director of Histopathology Cleveland Biolabs, Inc 73 High Street Buffalo, NY 14203 phone:716-849-6810 ext.354 fax:716-849-6817 e-mail: AGleiberman@cbiolabs.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Chirayu Sharma Sent: Tuesday, January 01, 2013 5:18 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Regarding a problem in manual paraffin embedding Hello Histonetters I am having a problem in Manual Paraffin Embedding of Embryonic Day 16-18 mice heads. My protocol for Paraffin embedding is Fix in 4%NBF for 2 days followed by rinsing the whole head of mice in running water for overnight. Series of ethanol wash 50% -4 hrs 70% 4 hrs 96% 4 hrs 100% 4 hrs Xylene-5 hours Series of Paraffin wash each consisting of 30 minutes interval till the smell of xylene is not there in the Paraffin bath. Finally prepare the block. My problem is even after putting in Xylene for 5 hours I didn't see clearing of mice heads. I tried with one spare mice heads and kept in xylene for 8 hours but I did not see clearing too. Later I put the same head still without clearing in Parafin and prepare the block, I found the head to be soft and the initial part of the head facing the microtome is white. And flecks of wax could be removed easily from the top of tissue. Another problem is even these tissues are able to cut in some instances the sections tear when putting in water bath and they look something whitish appearance when placed in slides. My problem is I cannot remove the brain and skin for penetration of xylene as my study area does not allow me. It would be so nice if somebody would share me their Manual Paraffin Embedding protocol of whole head of mice in embryonic day 17-18 and Postnatal day 0 and 1 so that I can get rid of these problems. Chirayu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information. From madeleinehuey <@t> gmail.com Wed Jan 2 13:10:32 2013 From: madeleinehuey <@t> gmail.com (Madeleine Huey) Date: Wed Jan 2 13:10:37 2013 Subject: [Histonet] Re: Histonet Digest, Vol 110, Issue 2 In-Reply-To: <50e475d2.e4b6ec0a.5c35.5532SMTPIN_ADDED_MISSING@mx.google.com> References: <50e475d2.e4b6ec0a.5c35.5532SMTPIN_ADDED_MISSING@mx.google.com> Message-ID: Chirayu, I am not surprise you have this problem, it's a classic issue for many lab (if they don't do research). Any time if Mouse embryo are older than Day 13 (E13), you need a small sharp scissor to slit open the mouse neck/skull & abdominal (> E13) in order to have properly fixation. It got nothing to do with tissue processing. You can process overnight (1hr - 1.5 hr per steps). Good Luck! madeleine_h@elcaminohospital.org On Wed, Jan 2, 2013 at 10:00 AM, wrote: > From: Chirayu Sharma > To: histonet@lists.utsouthwestern.edu > Sent: Tuesday, January 1, 2013 5:17 PM > Subject: [Histonet] Regarding a problem in manual paraffin embedding > > Hello Histonetters > > I am having a problem in Manual Paraffin Embedding of Embryonic Day 16-18 > mice heads. My protocol for Paraffin embedding is > > Fix in 4%NBF for 2 days followed by rinsing the whole head of mice in > running water for overnight. > > Series of ethanol wash > > 50% -4 hrs > > 70% 4 hrs > > 96% 4 hrs > > 100% 4 hrs > > Xylene-5 hours > > Series of Paraffin wash each consisting of 30 minutes interval till the > smell of xylene is not there in the Paraffin bath. Finally prepare the > block. > > My problem is even after putting in Xylene for 5 hours I didn?t see > clearing of mice heads. I tried with one spare mice heads and kept in > xylene for 8 hours but I did not see clearing too. Later I put the same > head still without clearing in Parafin and prepare the block, I found the > head to be soft and the initial part of the head facing the microtome is > white. And flecks of wax could be removed easily from the top of tissue. > > Another problem is even these tissues are able to cut in some instances the > sections tear when putting in water bath and they look something whitish > appearance when placed in slides. > > My problem is I cannot remove the brain and skin for penetration of xylene > as my study area does not allow me. > > It would be so nice if somebody would share me their Manual Paraffin > Embedding protocol of whole head of mice in embryonic day 17-18 and > Postnatal day 0 and 1 so that I can get rid of these problems. > > Chirayu From mbplab <@t> yahoo.com Wed Jan 2 13:47:49 2013 From: mbplab <@t> yahoo.com (Mary Benoit) Date: Wed Jan 2 13:47:53 2013 Subject: [Histonet] Glyco-Fixx Message-ID: <1357156069.39106.YahooMailNeo@web142502.mail.bf1.yahoo.com> Need help solving a fixation/processing problem.? We are a regional reference Pathology Lab and we recently began receiving GI biosies in Glyco-fix from Thermo Scientific.??Our standard processing for small biopsies is formalin 2 changes for 1hour and 1.5 hours; 70% for 15 minutes; 95% 2 changes 15 min each; 100% 3 changes 30 min each; xylene 2 changes 30 min each and paraffin 3 changes 30 min each.? Theis has worked great for years with my specimens received in formalin.? Now the biopsies received in glycol-fix have no nuclear detail, look "muddy" and I am not sure what to do or what to change in my protocol.? The pathologists are speaking with the clinicians, but it isa P.R. thing and this must be handled the right way.? I know the fixative is mostly alcohol blend with a little glyoxal, but even post fixing in 10%NBF is not helping.? Suggestions please! Mary F Benoit mbplab@yahoo.com The Pathology Laboratory Lake Charles, LA From LPaveli1 <@t> hurleymc.com Wed Jan 2 14:11:38 2013 From: LPaveli1 <@t> hurleymc.com (Lynette Pavelich) Date: Wed Jan 2 14:11:46 2013 Subject: [Histonet] Glyco-Fixx In-Reply-To: <1357156069.39106.YahooMailNeo@web142502.mail.bf1.yahoo.com> References: <1357156069.39106.YahooMailNeo@web142502.mail.bf1.yahoo.com> Message-ID: <89F4666A496DC949A819ECC40E11C867BF56F1A1@EXCHANGEMB1.hmc.hurleymc.com> I'm thinking that the Glyoxal fixative may not be your problem. When I have had experience with "muddy", I often have to look at our 100's and clearants. Sounds like perhaps you may have some water contamination going on. I would start there. What kind of processor are you using? Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 ph: 810.262.9948 mobile: 810.444.7966 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Mary Benoit [mbplab@yahoo.com] Sent: Wednesday, January 02, 2013 2:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Glyco-Fixx Need help solving a fixation/processing problem. We are a regional reference Pathology Lab and we recently began receiving GI biosies in Glyco-fix from Thermo Scientific. Our standard processing for small biopsies is formalin 2 changes for 1hour and 1.5 hours; 70% for 15 minutes; 95% 2 changes 15 min each; 100% 3 changes 30 min each; xylene 2 changes 30 min each and paraffin 3 changes 30 min each. Theis has worked great for years with my specimens received in formalin. Now the biopsies received in glycol-fix have no nuclear detail, look "muddy" and I am not sure what to do or what to change in my protocol. The pathologists are speaking with the clinicians, but it isa P.R. thing and this must be handled the right way. I know the fixative is mostly alcohol blend with a little glyoxal, but even post fixing in 10%NBF is not helping. Suggestions please! Mary F Benoit mbplab@yahoo.com The Pathology Laboratory Lake Charles, LA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brannon <@t> alliedsearchpartners.com Wed Jan 2 15:33:23 2013 From: brannon <@t> alliedsearchpartners.com (Brannon Owens) Date: Wed Jan 2 15:33:31 2013 Subject: [Histonet] Cytoprep Technician opening near Hicksville, NY Message-ID: Job opening: Cytoprep Technician Location: Hicksville, NY Schedule: full time/day shift NY license required To view a complete list of Allied Search Partners current openings go to: http://www.alliedsearchpartners.com/careers.php -- Brannon Owens Recruitment Manager Allied Search Partners LinkedIn: http://www.linkedin.com/pub/brannon-owens/28/528/823 http://www.alliedsearchpartners.com From chak_bou <@t> yahoo.com Thu Jan 3 08:41:42 2013 From: chak_bou <@t> yahoo.com (Chakib Boussahmain) Date: Thu Jan 3 08:41:51 2013 Subject: [Histonet] Anti-MYB4 Message-ID: <1357224102.8616.YahooMailClassic@web160801.mail.bf1.yahoo.com> Hi Histonet, ?Does anyone uses Anti-MYB4 antibody? If so, can you tell me the vendor? Thank you so much! Chakib Boussahmain Histotechnologist(HTL) ASCP From mucram11 <@t> comcast.net Thu Jan 3 10:06:01 2013 From: mucram11 <@t> comcast.net (Pam Marcum) Date: Thu Jan 3 10:06:09 2013 Subject: [Histonet] Pam Barker Please Contact Me Message-ID: <1421694358.59181.1357229161123.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Pam Marcum - UAMS From mucram11 <@t> comcast.net Thu Jan 3 10:51:40 2013 From: mucram11 <@t> comcast.net (Pam Marcum) Date: Thu Jan 3 10:51:57 2013 Subject: [Histonet] Traveling Histologists Message-ID: <1295483939.60906.1357231900400.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Could anyone with a good company for temporary histologists (travelers) please contact me privately.? We are in Little Rock AR. Pam Marcum From b427297 <@t> aol.com Thu Jan 3 12:24:50 2013 From: b427297 <@t> aol.com (Jackie O'Connor) Date: Thu Jan 3 12:24:57 2013 Subject: [Histonet] Contract Histologists in Europe In-Reply-To: <1295483939.60906.1357231900400.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> References: <1295483939.60906.1357231900400.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Message-ID: <8CFB7F6C0D3C0A3-16D8-35CA@webmail-d097.sysops.aol.com> A friend has asked I query about the availability of contract histology technicians in Europe. Do they exist? Is there a US company out there who would contract a histotech to Europe for a short term assignment (several months - year) or is there a European agency who could provide temporary histology support? Thanks From mkent <@t> dermpathlab.com Thu Jan 3 12:41:07 2013 From: mkent <@t> dermpathlab.com (Michael Kent) Date: Thu Jan 3 12:43:39 2013 Subject: [Histonet] 88305TC starting to hit the fan... In-Reply-To: <50D1B3D0.7400.0077.1@harthosp.org> References: <27EB7FFB1A15F549B17F79B1A856C70BAD5310@dlcs-sbs1.DPLCS.intra> <50D1B3D0.7400.0077.1@harthosp.org> Message-ID: <27EB7FFB1A15F549B17F79B1A856C70BB45170@dlcs-sbs1.DPLCS.intra> Hi Richard (and Others), We are investigating how independent labs can cooperate to lower costs and pool certain resources to mutually benefit from our strengths and meet common needs. If you are interested, we'd like to discuss in greater detail. Best and Happy New Year to All, Michael Kent, MS, PhD Translational Scientist Dermatopathology Laboratory of Central States Adjunct Assistant Professor of Dermatology Wright State University Boonshoft School of Medicine 7835 Paragon Rd. Dayton OH 45459 (937) 436-4152 www.dermpathlab.com Confidentiality Notice: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. Thank you. -----Original Message----- From: Richard Cartun [mailto:Rcartun@harthosp.org] Sent: Wednesday, December 19, 2012 12:32 PM To: Michael Kent Subject: RE: [Histonet] 88305TC starting to hit the fan... Hi Michael: Out of curiosity, how can you help? Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-2204 Fax >>> "Michael Kent" 12/18/2012 4:25 PM >>> Fellow Histoneters, Please feel free to contact me if your lab is in trouble from these cuts. We may be able to help. Also- we are hiring a Histotech in the Troy Michigan area, if you know anyone interested. Best and Happy Holidays, Michael Kent, MS, PhD Translational Scientist Dermatopathology Laboratory of Central States Adjunct Assistant Professor of Dermatology Wright State University Boonshoft School of Medicine 7835 Paragon Rd. Dayton OH 45459 (937) 436-4152 www.dermpathlab.com Confidentiality Notice: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From csheil <@t> jcu.edu Thu Jan 3 13:23:17 2013 From: csheil <@t> jcu.edu (Christopher Sheil) Date: Thu Jan 3 13:22:00 2013 Subject: [Histonet] staining site of bone deposition on live specimens Message-ID: <32D4A928-97BC-4373-B3CA-190D12286291@jcu.edu> Hello, I study the timing of formation/ossification of skeletal elements in various vertebrate groups; currently I am working with Xenopus laevis. Most all of my work has described ossification sequences for cranial elements by documenting which elements are present at various stages of development. I would like to address issues of intraspecific variation in timing of ossification, so here is my question: Are there any dyes or stains that can be administered to live aquatic amphibians and that will bind to bone at the site of deposition of the mineral matrix? The trick is that I would like to apply this dye/ stain at regular intervals, so it must be possible to apply them without killing the animal---visualization of the sites of deposition will be conducted after the study is completed (at which point I will euthanize the treated tadpoles). I greatly appreciate any help with this issue. Sincerely, -Christopher Christopher Sheil Graduate Coordinator Department of Biology John Carroll University 20700 North Park Boulevard University Heights, OH 44118 Email: csheil@jcu.edu Phone: 216.397.3088 From tfraser <@t> olympicmedical.org Thu Jan 3 13:22:46 2013 From: tfraser <@t> olympicmedical.org (Tasha Fraser) Date: Thu Jan 3 13:23:22 2013 Subject: [Histonet] H Pylori Stain Message-ID: <5290DE49B23B6240BC0DF14FC8142D1901CB907F@is-210vs.olympicmedical.local> Hi everyone! Does anyone have a good h-pylori stain? We currently use the Steiner and Steiner Stain. We've done some testing with the Alcian Yellow-Toluidine Blue Method, but not getting the same results as the book shows. Does anyone have any suggestions please. Tasha Fraser , HT (ASCP) Olympic Medical Center Port Angeles, WA ------------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended individual(s) named above and may contain confidential, privileged, and/or protected information. Any unauthorized review, use, disclosure, copying, or distribution of its contents is prohibited. If you are not the intended recipient, you have received this email in error. If so, please notify the sender immediately by reply email and delete/destroy the original and all copies of this communication. Also know that Internet e-mail is not secure. In choosing to communicate with Olympic Medical Center by email you will assume these confidentiality risks. Internet messages may become corrupted, incomplete, or may incorrectly identify the sender. From joelleweaver <@t> hotmail.com Thu Jan 3 13:28:04 2013 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Thu Jan 3 13:28:12 2013 Subject: [Histonet] H Pylori Stain In-Reply-To: <5290DE49B23B6240BC0DF14FC8142D1901CB907F@is-210vs.olympicmedical.local> References: <5290DE49B23B6240BC0DF14FC8142D1901CB907F@is-210vs.olympicmedical.local> Message-ID: If you want a special stain- How about the good 'ole Giemsa with Jenner's? Works fine, is cheap and quick. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: tfraser@olympicmedical.org > To: histonet@lists.utsouthwestern.edu > Date: Thu, 3 Jan 2013 11:22:46 -0800 > Subject: [Histonet] H Pylori Stain > > Hi everyone! Does anyone have a good h-pylori stain? We currently use > the Steiner and Steiner Stain. We've done some testing with the Alcian > Yellow-Toluidine Blue Method, but not getting the same results as the > book shows. Does anyone have any suggestions please. > > > > Tasha Fraser , HT (ASCP) > > Olympic Medical Center > > Port Angeles, WA > > > > > ------------------------------------------------------------------------- > Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended individual(s) named above and may contain confidential, privileged, and/or protected information. Any unauthorized review, use, disclosure, copying, or distribution of its contents is prohibited. If you are not the intended recipient, you have received this email in error. If so, please notify the sender immediately by reply email and delete/destroy the original and all copies of this communication. Also know that Internet e-mail is not secure. In choosing to communicate with Olympic Medical Center by email you will assume these confidentiality risks. Internet messages may become corrupted, incomplete, or may incorrectly identify the sender. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MICHELLE.LAMPHERE <@t> childrens.com Thu Jan 3 14:16:30 2013 From: MICHELLE.LAMPHERE <@t> childrens.com (Michelle Lamphere) Date: Thu Jan 3 14:16:33 2013 Subject: [Histonet] BK Virus Controls Message-ID: Is there anybody that has BK Virus (IHC) control tissue that they would be willing to share? Our processes are changing and we are about to start performing this test more frequently. Unfortunately, we very rarely have a positive case and so I am quite limited on control tissue. Thank you in advance. Michelle M Lamphere, HT (ASCP) Senior Tech, Histology Children's Medical Center 1935 Medical District Drive Dallas, TX 75235 Office :214-456-2798 Histology: 214-456-2318 Fax: 214-456-0779 Please consider the environment before printing this e-mail This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. Please consider the environment before printing this e-mail. This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. From robert_schoonhoven <@t> yahoo.com Thu Jan 3 14:19:10 2013 From: robert_schoonhoven <@t> yahoo.com (Robert Schoonhoven) Date: Thu Jan 3 14:19:14 2013 Subject: [Histonet] staining site of bone deposition on live specimens In-Reply-To: <32D4A928-97BC-4373-B3CA-190D12286291@jcu.edu> References: <32D4A928-97BC-4373-B3CA-190D12286291@jcu.edu> Message-ID: <1357244350.82922.YahooMailNeo@web126206.mail.ne1.yahoo.com> In the past we used tetracycline on rats.? Not sure how that would affect live aquatic amphibians. ? ? ? Robert Schoonhoven, HT/HTL (ASCP) ________________________________ From: Christopher Sheil To: histonet@lists.utsouthwestern.edu Sent: Thursday, January 3, 2013 2:23 PM Subject: [Histonet] staining site of bone deposition on live specimens.? Hello, ? ? I study the timing of formation/ossification of skeletal elements in various vertebrate groups; currently I am working with Xenopus laevis.? Most all of my work has described ossification sequences for cranial elements by documenting which elements are present at various stages of development.? I would like to address issues of intraspecific variation in timing of ossification, so here is my question: Are there any dyes or stains that can be administered to live aquatic amphibians and that will bind to bone at the site of deposition of the mineral matrix?? The trick is that I would like to apply this dye/stain at regular intervals, so it must be possible to apply them without killing the animal---visualization of the sites of deposition will be conducted after the study is completed (at which point I will euthanize the treated tadpoles). I greatly appreciate any help with this issue. Sincerely, -Christopher Christopher Sheil Graduate Coordinator Department of Biology John Carroll University 20700 North Park Boulevard University Heights, OH? 44118 Email: csheil@jcu.edu Phone: 216.397.3088 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Jan 3 14:26:38 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jan 3 14:26:43 2013 Subject: [Histonet] H Pylori Stain In-Reply-To: <5290DE49B23B6240BC0DF14FC8142D1901CB907F@is-210vs.olympicmedical.local> References: <5290DE49B23B6240BC0DF14FC8142D1901CB907F@is-210vs.olympicmedical.local> Message-ID: <1357244798.88524.YahooMailNeo@web163106.mail.bf1.yahoo.com> You can find my procedure at http://www.histosearch.com/rene.html Ren? J. From: Tasha Fraser To: "histonet@lists.utsouthwestern.edu" Sent: Thursday, January 3, 2013 2:22 PM Subject: [Histonet] H Pylori Stain Hi everyone!? Does anyone have a good h-pylori stain?? We currently use the Steiner and Steiner Stain.? We've done some testing with the Alcian Yellow-Toluidine Blue Method, but not getting the same results as the book shows.? Does anyone have any suggestions please. Tasha Fraser , HT (ASCP) Olympic Medical Center Port Angeles, WA ------------------------------------------------------------------------- Confidentiality Notice:? This e-mail message, including any attachments, is for the sole use of the intended individual(s) named above and may contain confidential, privileged, and/or protected information.? Any unauthorized review, use, disclosure, copying, or distribution of its contents is prohibited.? If you are not the intended recipient, you have received this email in error.? If so, please notify the sender immediately by reply email and delete/destroy the original and all copies of this communication.? Also know that Internet e-mail is not secure.? In choosing to communicate with Olympic Medical Center by email you will assume these confidentiality risks.? Internet messages may become corrupted, incomplete, or may incorrectly identify the sender. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LPaveli1 <@t> hurleymc.com Fri Jan 4 09:21:49 2013 From: LPaveli1 <@t> hurleymc.com (Lynette Pavelich) Date: Fri Jan 4 09:21:58 2013 Subject: [Histonet] RE: H Pylori Stain In-Reply-To: <5290DE49B23B6240BC0DF14FC8142D1901CB907F@is-210vs.olympicmedical.local> References: <5290DE49B23B6240BC0DF14FC8142D1901CB907F@is-210vs.olympicmedical.local> Message-ID: <89F4666A496DC949A819ECC40E11C867BF56F413@EXCHANGEMB1.hmc.hurleymc.com> If you are currently doing IHC, you might want to consider working up the H. Pylori with it. Our docs prefer it, as it is easier to identify them on the slide. Lynette ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Tasha Fraser [tfraser@olympicmedical.org] Sent: Thursday, January 03, 2013 2:22 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] H Pylori Stain Hi everyone! Does anyone have a good h-pylori stain? We currently use the Steiner and Steiner Stain. We've done some testing with the Alcian Yellow-Toluidine Blue Method, but not getting the same results as the book shows. Does anyone have any suggestions please. Tasha Fraser , HT (ASCP) Olympic Medical Center Port Angeles, WA ------------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended individual(s) named above and may contain confidential, privileged, and/or protected information. Any unauthorized review, use, disclosure, copying, or distribution of its contents is prohibited. If you are not the intended recipient, you have received this email in error. If so, please notify the sender immediately by reply email and delete/destroy the original and all copies of this communication. Also know that Internet e-mail is not secure. In choosing to communicate with Olympic Medical Center by email you will assume these confidentiality risks. Internet messages may become corrupted, incomplete, or may incorrectly identify the sender. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From katelin09htl <@t> gmail.com Fri Jan 4 09:46:36 2013 From: katelin09htl <@t> gmail.com (Katelin Lester) Date: Fri Jan 4 09:46:39 2013 Subject: [Histonet] H Pylori Stain In-Reply-To: <5290DE49B23B6240BC0DF14FC8142D1901CB907F@is-210vs.olympicmedical.local> References: <5290DE49B23B6240BC0DF14FC8142D1901CB907F@is-210vs.olympicmedical.local> Message-ID: We use the HP Blue stain by Anatech. Katelin Lester, HTL On Jan 3, 2013 11:23 AM, "Tasha Fraser" wrote: > Hi everyone! Does anyone have a good h-pylori stain? We currently use > the Steiner and Steiner Stain. We've done some testing with the Alcian > Yellow-Toluidine Blue Method, but not getting the same results as the > book shows. Does anyone have any suggestions please. > > > > Tasha Fraser , HT (ASCP) > > Olympic Medical Center > > Port Angeles, WA > > > > > ------------------------------------------------------------------------- > Confidentiality Notice: This e-mail message, including any attachments, > is for the sole use of the intended individual(s) named above and may > contain confidential, privileged, and/or protected information. Any > unauthorized review, use, disclosure, copying, or distribution of its > contents is prohibited. If you are not the intended recipient, you have > received this email in error. If so, please notify the sender immediately > by reply email and delete/destroy the original and all copies of this > communication. Also know that Internet e-mail is not secure. In choosing > to communicate with Olympic Medical Center by email you will assume these > confidentiality risks. Internet messages may become corrupted, > incomplete, or may incorrectly identify the sender. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From tfraser <@t> olympicmedical.org Fri Jan 4 11:37:10 2013 From: tfraser <@t> olympicmedical.org (Tasha Fraser) Date: Fri Jan 4 11:37:34 2013 Subject: [Histonet] Helicobater Pylori QC block and/or slides Message-ID: <5290DE49B23B6240BC0DF14FC8142D1901CB9083@is-210vs.olympicmedical.local> Does anyone have a good suggestion for a good HP control block or slides? Tasha Fraser, HT (ASCP) Olympic Medical Center Port Angeles, WA ------------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended individual(s) named above and may contain confidential, privileged, and/or protected information. Any unauthorized review, use, disclosure, copying, or distribution of its contents is prohibited. If you are not the intended recipient, you have received this email in error. If so, please notify the sender immediately by reply email and delete/destroy the original and all copies of this communication. Also know that Internet e-mail is not secure. In choosing to communicate with Olympic Medical Center by email you will assume these confidentiality risks. Internet messages may become corrupted, incomplete, or may incorrectly identify the sender. From madeleinehuey <@t> gmail.com Fri Jan 4 12:19:14 2013 From: madeleinehuey <@t> gmail.com (Madeleine Huey) Date: Fri Jan 4 12:19:19 2013 Subject: [Histonet] Re: Histonet Digest, Vol 110, Issue 4 In-Reply-To: <50e718cc.0574650a.784f.ffff8034SMTPIN_ADDED_MISSING@mx.google.com> References: <50e718cc.0574650a.784f.ffff8034SMTPIN_ADDED_MISSING@mx.google.com> Message-ID: Hi Tasha, Warthin-Starry will work very well. We used Dako Autostainer - Artisan in our lab, but manual will work as well. Good Luck! Supervisor - Pathology (IPOX & Histology) Madeleine Huey BS, MT (CSMT), HTL & QIHC (ASCP) madeleine_h@elcaminohospital.org Message: 4 Date: Thu, 3 Jan 2013 11:22:46 -0800 From: Tasha Fraser Subject: [Histonet] H Pylori Stain To: "histonet@lists.utsouthwestern.edu" Message-ID: <5290DE49B23B6240BC0DF14FC8142D1901CB907F@is-210vs.olympicmedical.local> Content-Type: text/plain; charset="us-ascii" Hi everyone! Does anyone have a good h-pylori stain? We currently use the Steiner and Steiner Stain. We've done some testing with the Alcian Yellow-Toluidine Blue Method, but not getting the same results as the book shows. Does anyone have any suggestions please. Tasha Fraser , HT (ASCP) Olympic Medical Center Port Angeles, WA From katelin09htl <@t> gmail.com Fri Jan 4 12:22:34 2013 From: katelin09htl <@t> gmail.com (Katelin Lester) Date: Fri Jan 4 12:22:39 2013 Subject: [Histonet] Helicobater Pylori QC block and/or slides In-Reply-To: <5290DE49B23B6240BC0DF14FC8142D1901CB9083@is-210vs.olympicmedical.local> References: <5290DE49B23B6240BC0DF14FC8142D1901CB9083@is-210vs.olympicmedical.local> Message-ID: We use human precut control slides from Newcomer Supply. Katelin Lester, HTL On Jan 4, 2013 9:37 AM, "Tasha Fraser" wrote: > Does anyone have a good suggestion for a good HP control block or > slides? > > > > Tasha Fraser, HT (ASCP) > > Olympic Medical Center > > Port Angeles, WA > > > ------------------------------------------------------------------------- > Confidentiality Notice: This e-mail message, including any attachments, > is for the sole use of the intended individual(s) named above and may > contain confidential, privileged, and/or protected information. Any > unauthorized review, use, disclosure, copying, or distribution of its > contents is prohibited. If you are not the intended recipient, you have > received this email in error. If so, please notify the sender immediately > by reply email and delete/destroy the original and all copies of this > communication. Also know that Internet e-mail is not secure. In choosing > to communicate with Olympic Medical Center by email you will assume these > confidentiality risks. Internet messages may become corrupted, > incomplete, or may incorrectly identify the sender. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From LPaveli1 <@t> hurleymc.com Fri Jan 4 12:47:16 2013 From: LPaveli1 <@t> hurleymc.com (Lynette Pavelich) Date: Fri Jan 4 12:47:22 2013 Subject: [Histonet] Helicobater Pylori QC block and/or slides In-Reply-To: References: <5290DE49B23B6240BC0DF14FC8142D1901CB9083@is-210vs.olympicmedical.local>, Message-ID: <89F4666A496DC949A819ECC40E11C867BF56F481@EXCHANGEMB1.hmc.hurleymc.com> If you join the Michigan Society at : www.mihisto.org you will have access to the society's FREE known, tested positive control blocks. They have many different control blocks, and I know for a fact that they have a good supply of HP blocks! The cost of $20 to join is way less expensive than purchasing slides. After joining, go to "Members" and you will see the link to control blocks. Members also receive the MSH "Mikro-Graf", the NSH award winning newsletter of the year! Please note that they ask that you request 1 control block of each per request to assure help to as many techs as possible! Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 ph: 810.262.9948 mobile: 810.444.7966 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Katelin Lester [katelin09htl@gmail.com] Sent: Friday, January 04, 2013 1:22 PM To: Tasha Fraser Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Helicobater Pylori QC block and/or slides We use human precut control slides from Newcomer Supply. Katelin Lester, HTL On Jan 4, 2013 9:37 AM, "Tasha Fraser" wrote: > Does anyone have a good suggestion for a good HP control block or > slides? > > > > Tasha Fraser, HT (ASCP) > > Olympic Medical Center > > Port Angeles, WA > > > ------------------------------------------------------------------------- > Confidentiality Notice: This e-mail message, including any attachments, > is for the sole use of the intended individual(s) named above and may > contain confidential, privileged, and/or protected information. Any > unauthorized review, use, disclosure, copying, or distribution of its > contents is prohibited. If you are not the intended recipient, you have > received this email in error. If so, please notify the sender immediately > by reply email and delete/destroy the original and all copies of this > communication. Also know that Internet e-mail is not secure. In choosing > to communicate with Olympic Medical Center by email you will assume these > confidentiality risks. Internet messages may become corrupted, > incomplete, or may incorrectly identify the sender. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Fri Jan 4 13:01:30 2013 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Fri Jan 4 13:01:34 2013 Subject: [Histonet] Helicobater Pylori QC block and/or slides In-Reply-To: <89F4666A496DC949A819ECC40E11C867BF56F481@EXCHANGEMB1.hmc.hurleymc.com> References: <5290DE49B23B6240BC0DF14FC8142D1901CB9083@is-210vs.olympicmedical.local>, , , <89F4666A496DC949A819ECC40E11C867BF56F481@EXCHANGEMB1.hmc.hurleymc.com> Message-ID: The Michigan Society is awesome! Joelle Weaver MAOM, HTL (ASCP) QIHC > From: LPaveli1@hurleymc.com > Date: Fri, 4 Jan 2013 18:47:16 +0000 > To: katelin09htl@gmail.com; tfraser@olympicmedical.org > Subject: RE: [Histonet] Helicobater Pylori QC block and/or slides > CC: histonet@lists.utsouthwestern.edu > > If you join the Michigan Society at : www.mihisto.org you will have access to the society's FREE known, tested positive control blocks. They have many different control blocks, and I know for a fact that they have a good supply of HP blocks! > The cost of $20 to join is way less expensive than purchasing slides. After joining, go to "Members" and you will see the link to control blocks. Members also receive the MSH "Mikro-Graf", the NSH award winning newsletter of the year! > > Please note that they ask that you request 1 control block of each per request to assure help to as many techs as possible! > > Lynette > > Lynette Pavelich, HT(ASCP) > Histology Supervisor > Hurley Medical Center > One Hurley Plaza > Flint, MI 48503 > > ph: 810.262.9948 > mobile: 810.444.7966 > > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Katelin Lester [katelin09htl@gmail.com] > Sent: Friday, January 04, 2013 1:22 PM > To: Tasha Fraser > Cc: Histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Helicobater Pylori QC block and/or slides > > We use human precut control slides from Newcomer Supply. > > Katelin Lester, HTL > On Jan 4, 2013 9:37 AM, "Tasha Fraser" wrote: > > > Does anyone have a good suggestion for a good HP control block or > > slides? > > > > > > > > Tasha Fraser, HT (ASCP) > > > > Olympic Medical Center > > > > Port Angeles, WA > > > > > > ------------------------------------------------------------------------- > > Confidentiality Notice: This e-mail message, including any attachments, > > is for the sole use of the intended individual(s) named above and may > > contain confidential, privileged, and/or protected information. Any > > unauthorized review, use, disclosure, copying, or distribution of its > > contents is prohibited. If you are not the intended recipient, you have > > received this email in error. If so, please notify the sender immediately > > by reply email and delete/destroy the original and all copies of this > > communication. Also know that Internet e-mail is not secure. In choosing > > to communicate with Olympic Medical Center by email you will assume these > > confidentiality risks. Internet messages may become corrupted, > > incomplete, or may incorrectly identify the sender. > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Fri Jan 4 13:20:00 2013 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Jan 4 13:20:14 2013 Subject: [Histonet] Helicobater Pylori QC block and/or slides In-Reply-To: <5290DE49B23B6240BC0DF14FC8142D1901CB9083@is-210vs.olympicmedical.local> References: <5290DE49B23B6240BC0DF14FC8142D1901CB9083@is-210vs.olympicmedical.local> Message-ID: <50E6E50F.7400.0077.0@harthosp.org> I use gastric resection tissue that is H. pylori positive. You can use biopsy tissue as well, but I would be careful to not cut-through the tissue in case retrospective testing is ordered. You should always try to use tissue that is fixed and processed in your own laboratory. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-2204 Fax >>> Tasha Fraser 1/4/2013 12:37 PM >>> Does anyone have a good suggestion for a good HP control block or slides? Tasha Fraser, HT (ASCP) Olympic Medical Center Port Angeles, WA ------------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended individual(s) named above and may contain confidential, privileged, and/or protected information. Any unauthorized review, use, disclosure, copying, or distribution of its contents is prohibited. If you are not the intended recipient, you have received this email in error. If so, please notify the sender immediately by reply email and delete/destroy the original and all copies of this communication. Also know that Internet e-mail is not secure. In choosing to communicate with Olympic Medical Center by email you will assume these confidentiality risks. Internet messages may become corrupted, incomplete, or may incorrectly identify the sender. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LPaveli1 <@t> hurleymc.com Fri Jan 4 13:47:46 2013 From: LPaveli1 <@t> hurleymc.com (Lynette Pavelich) Date: Fri Jan 4 13:48:55 2013 Subject: [Histonet] Helicobater Pylori QC block and/or slides In-Reply-To: <50E6E50F.7400.0077.0@harthosp.org> References: <5290DE49B23B6240BC0DF14FC8142D1901CB9083@is-210vs.olympicmedical.local>, <50E6E50F.7400.0077.0@harthosp.org> Message-ID: <89F4666A496DC949A819ECC40E11C867BF56F4B8@EXCHANGEMB1.hmc.hurleymc.com> Dr. Cartun is absolutely correct in always trying to use positive tissue that has been processed in your lab using the same process as your patient tissues. The control blocks offered through the Michigan society have been donated by various laboratories in the state, and have been tested prior to donation. Before mailing a control block, that block is cut and tested again, and the slide accompanies the block to the recipient. When I donate a block to the society to be used for IHC, I supply the information of what type of fixative and how long it remained in it prior to processing as this can be critical to IHC. With all due respect, these controls are only offered as an alternative to those who have trouble locating/budget constrictions. Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 ph: 810.262.9948 mobile: 810.444.7966 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Richard Cartun [Rcartun@harthosp.org] Sent: Friday, January 04, 2013 2:20 PM To: Histonet@lists.utsouthwestern.edu; Tasha Fraser Subject: Re: [Histonet] Helicobater Pylori QC block and/or slides I use gastric resection tissue that is H. pylori positive. You can use biopsy tissue as well, but I would be careful to not cut-through the tissue in case retrospective testing is ordered. You should always try to use tissue that is fixed and processed in your own laboratory. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-2204 Fax >>> Tasha Fraser 1/4/2013 12:37 PM >>> Does anyone have a good suggestion for a good HP control block or slides? Tasha Fraser, HT (ASCP) Olympic Medical Center Port Angeles, WA ------------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended individual(s) named above and may contain confidential, privileged, and/or protected information. Any unauthorized review, use, disclosure, copying, or distribution of its contents is prohibited. If you are not the intended recipient, you have received this email in error. If so, please notify the sender immediately by reply email and delete/destroy the original and all copies of this communication. Also know that Internet e-mail is not secure. In choosing to communicate with Olympic Medical Center by email you will assume these confidentiality risks. Internet messages may become corrupted, incomplete, or may incorrectly identify the sender. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Fri Jan 4 14:39:12 2013 From: joelleweaver <@t> hotmail.com (=?utf-8?B?am9lbGxld2VhdmVyQGhvdG1haWwuY29t?=) Date: Fri Jan 4 14:39:23 2013 Subject: =?utf-8?B?UmU6IFtIaXN0b25ldF0gSGVsaWNvYmF0ZXIgUHlsb3JpIFFDIGJsb2NrIGFuZC9v?= =?utf-8?B?ciBzbGlkZXM=?= Message-ID: Still a nice service, despite your points about its limitations. Many areas don't even have that option! It's a good thing for those who are having trouble. Sent from my Verizon Wireless 4G LTE Smartphone ----- Reply message ----- From: "Lynette Pavelich" To: "Richard Cartun" , "histonet@lists.utsouthwestern.edu" , "Tasha Fraser" Subject: [Histonet] Helicobater Pylori QC block and/or slides Date: Fri, Jan 4, 2013 1:47 pm Dr. Cartun is absolutely correct in always trying to use positive tissue that has been processed in your lab using the same process as your patient tissues. The control blocks offered through the Michigan society have been donated by various laboratories in the state, and have been tested prior to donation.. Before mailing a control block, that block is cut and tested again, and the slide accompanies the block to the recipient. When I donate a block to the society to be used for IHC, I supply the information of what type of fixative and how long it remained in it prior to processing as this can be critical to IHC. With all due respect, these controls are only offered as an alternative to those who have trouble locating/budget constrictions. Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 ph: 810.262.9948 mobile: 810.444.7966 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Richard Cartun [Rcartun@harthosp.org] Sent: Friday, January 04, 2013 2:20 PM To: Histonet@lists.utsouthwestern.edu; Tasha Fraser Subject: Re: [Histonet] Helicobater Pylori QC block and/or slides I use gastric resection tissue that is H. pylori positive. You can use biopsy tissue as well, but I would be careful to not cut-through the tissue in case retrospective testing is ordered. You should always try to use tissue that is fixed and processed in your own laboratory. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-2204 Fax >>> Tasha Fraser 1/4/2013 12:37 PM >>> Does anyone have a good suggestion for a good HP control block or slides? Tasha Fraser, HT (ASCP) Olympic Medical Center Port Angeles, WA ------------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended individual(s) named above and may contain confidential, privileged, and/or protected information. Any unauthorized review, use, disclosure, copying, or distribution of its contents is prohibited. If you are not the intended recipient, you have received this email in error. If so, please notify the sender immediately by reply email and delete/destroy the original and all copies of this communication. Also know that Internet e-mail is not secure. In choosing to communicate with Olympic Medical Center by email you will assume these confidentiality risks. Internet messages may become corrupted, incomplete, or may incorrectly identify the sender. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dunatrsd <@t> sbcglobal.net Fri Jan 4 15:28:33 2013 From: dunatrsd <@t> sbcglobal.net (dusko trajkovic) Date: Fri Jan 4 15:28:37 2013 Subject: [Histonet] PSCA Primary antibody In-Reply-To: <89F4666A496DC949A819ECC40E11C867BF56F481@EXCHANGEMB1.hmc.hurleymc.com> References: <5290DE49B23B6240BC0DF14FC8142D1901CB9083@is-210vs.olympicmedical.local>, <89F4666A496DC949A819ECC40E11C867BF56F481@EXCHANGEMB1.hmc.hurleymc.com> Message-ID: <1357334913.87679.YahooMailRC@web181704.mail.ne1.yahoo.com> Hallo Histonetters, Happy New Year!! I'm looking for?information on a PSCA (Prostate Specific Cell antigen) antibody that will work both in human and primate. If are are using this antibody, please contact me.?Any information would be appreciated. thanks Dusko Trajkovic 858-638-6202 From rheyna <@t> lumc.edu Fri Jan 4 16:00:35 2013 From: rheyna <@t> lumc.edu (Roger Heyna) Date: Fri Jan 4 16:00:41 2013 Subject: [Histonet] Pathologists' Names on H&E Labels Message-ID: <50E6FCA30200002300047B62@gwgwia2> For years, our lab's information system has been set up so that the name of the pathologist assigned to a case prints on our H&E labels to assist with sorting and grouping the slides after staining. As new pathologists join our team, concerns regarding this practice have come up. The pathologists are concerned that having their names on the slides pose medical-legal liability, and they want the names removed. I've yet to hear clear ramifications involved with having doctors' names on slides. What liability is really involved? We are a large, urban academic medical center with high specimen volumes. Our pathologists rotate through different sub-specialties, sometimes on a daily basis. For us, having the pathologists' names on the H&E labels seems to be the easiest way to get the H&E's to the correct pathologist. Are other labs printing the names of the pathologists on their H&E labels? Is anyone aware of any legal risk involved in this practice? Would anyone mind sharing how they sort and divide the slides before submitting them to their pathologists? Thank you, Roger Heyna, BS, HTL(ASCP) Loyola University Medical Center Maywood, IL From mike <@t> pathview.com Fri Jan 4 16:09:48 2013 From: mike <@t> pathview.com (Michael Mihalik) Date: Fri Jan 4 16:09:56 2013 Subject: [Histonet] Pathologists' Names on H&E Labels In-Reply-To: <50E6FCA30200002300047B62@gwgwia2> References: <50E6FCA30200002300047B62@gwgwia2> Message-ID: <00d401cdeac8$373150b0$a593f210$@pathview.com> Roger I've run into this exact issue as an LIS vendor. The concern was that the name on the slide would somehow be construed as the actual pathologist who performed the diagnosis, despite the fact that the LIS maintained the identity of the pathologist who actually signed off the case in it's database. It just 'disturbed' some pathologists. Long story short, unique initials were utilized instead and the politics of the situation evaporated. As a suggestion, should this continue to be an issue at your facility, why not use a numbering system or some other designator to identify a pathologist group/workgroup if you will (yes, it would be a group of 1) Feel free to email or call me separately if you'd like a few more details. Michael Mihalik PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Roger Heyna Sent: Friday, January 04, 2013 2:01 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Pathologists' Names on H&E Labels For years, our lab's information system has been set up so that the name of the pathologist assigned to a case prints on our H&E labels to assist with sorting and grouping the slides after staining. As new pathologists join our team, concerns regarding this practice have come up. The pathologists are concerned that having their names on the slides pose medical-legal liability, and they want the names removed. I've yet to hear clear ramifications involved with having doctors' names on slides. What liability is really involved? We are a large, urban academic medical center with high specimen volumes. Our pathologists rotate through different sub-specialties, sometimes on a daily basis. For us, having the pathologists' names on the H&E labels seems to be the easiest way to get the H&E's to the correct pathologist. Are other labs printing the names of the pathologists on their H&E labels? Is anyone aware of any legal risk involved in this practice? Would anyone mind sharing how they sort and divide the slides before submitting them to their pathologists? Thank you, Roger Heyna, BS, HTL(ASCP) Loyola University Medical Center Maywood, IL From Joyce.Weems <@t> emoryhealthcare.org Fri Jan 4 16:26:37 2013 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Fri Jan 4 16:26:43 2013 Subject: [Histonet] Pathologists' Names on H&E Labels In-Reply-To: <50E6FCA30200002300047B62@gwgwia2> References: <50E6FCA30200002300047B62@gwgwia2> Message-ID: We don't have names on slides but I wish we did!! My first thought is that there would be no more liability on having their name on the slide than on the report. I don't understand the concern. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Roger Heyna Sent: Friday, January 04, 2013 5:01 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Pathologists' Names on H&E Labels For years, our lab's information system has been set up so that the name of the pathologist assigned to a case prints on our H&E labels to assist with sorting and grouping the slides after staining. As new pathologists join our team, concerns regarding this practice have come up. The pathologists are concerned that having their names on the slides pose medical-legal liability, and they want the names removed. I've yet to hear clear ramifications involved with having doctors' names on slides. What liability is really involved? We are a large, urban academic medical center with high specimen volumes. Our pathologists rotate through different sub-specialties, sometimes on a daily basis. For us, having the pathologists' names on the H&E labels seems to be the easiest way to get the H&E's to the correct pathologist. Are other labs printing the names of the pathologists on their H&E labels? Is anyone aware of any legal risk involved in this practice? Would anyone mind sharing how they sort and divide the slides before submitting them to their pathologists? Thank you, Roger Heyna, BS, HTL(ASCP) Loyola University Medical Center Maywood, IL ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From vtobias <@t> uw.edu Fri Jan 4 17:21:28 2013 From: vtobias <@t> uw.edu (Victor A. Tobias) Date: Fri Jan 4 17:22:18 2013 Subject: [Histonet] CAP guideline for fixation time Message-ID: Per CAP guideline, in our reports of predictive markers(ER/PR/Her2), Pathologists need to check that the total fixation time of specimens is within the CAP guideline. Is this information readily available to the pathologists at the time of writing the report? How are others documenting this? Have a great weekend. Victor Victor Tobias HT(ASCP) Clinical Applications Analyst Harborview Medical Center Dept of Pathology Room NJB244 Seattle, WA 98104 vtobias@u.washington.edu 206-744-2735 206-744-8240 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. From hans <@t> histologistics.com Fri Jan 4 19:06:27 2013 From: hans <@t> histologistics.com (Hans B Snyder) Date: Fri Jan 4 19:06:31 2013 Subject: Fwd: [Histonet] Helicobater Pylori QC block and/or slides In-Reply-To: References: <5290DE49B23B6240BC0DF14FC8142D1901CB9083@is-210vs.olympicmedical.local> Message-ID: ---------- Forwarded message ---------- From: Hans B Snyder Date: Fri, Jan 4, 2013 at 5:32 PM Subject: Re: [Histonet] Helicobater Pylori QC block and/or slides To: Tasha Fraser We have a human colon tissue that is positive. On Fri, Jan 4, 2013 at 12:37 PM, Tasha Fraser wrote: > Does anyone have a good suggestion for a good HP control block or > slides? > > > > Tasha Fraser, HT (ASCP) > > Olympic Medical Center > > Port Angeles, WA > > > ------------------------------------------------------------------------- > Confidentiality Notice: This e-mail message, including any attachments, > is for the sole use of the intended individual(s) named above and may > contain confidential, privileged, and/or protected information. Any > unauthorized review, use, disclosure, copying, or distribution of its > contents is prohibited. If you are not the intended recipient, you have > received this email in error. If so, please notify the sender immediately > by reply email and delete/destroy the original and all copies of this > communication. Also know that Internet e-mail is not secure. In choosing > to communicate with Olympic Medical Center by email you will assume these > confidentiality risks. Internet messages may become corrupted, > incomplete, or may incorrectly identify the sender. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Hans B Snyder Histologistics 100 Barber Ave Worcester, MA 01606 508-308-7800 hans@histologistics.com -- Hans B Snyder Histologistics 100 Barber Ave Worcester, MA 01606 508-308-7800 hans@histologistics.com From hans <@t> histologistics.com Fri Jan 4 21:21:20 2013 From: hans <@t> histologistics.com (Hans B Snyder) Date: Fri Jan 4 21:21:28 2013 Subject: [Histonet] Helicobater Pylori QC block and/or slides In-Reply-To: References: <5290DE49B23B6240BC0DF14FC8142D1901CB9083@is-210vs.olympicmedical.local> Message-ID: <688D207C-281C-4008-A90C-B031BCB63C66@histologistics.com> We are a private histology lab that is willing to share. If you need slides or blocks let us know! thanks Sent from my iPad On Jan 4, 2013, at 8:06 PM, Hans B Snyder wrote: > > > ---------- Forwarded message ---------- > From: Hans B Snyder > Date: Fri, Jan 4, 2013 at 5:32 PM > Subject: Re: [Histonet] Helicobater Pylori QC block and/or slides > To: Tasha Fraser > > > We have a human colon tissue that is positive. > > > On Fri, Jan 4, 2013 at 12:37 PM, Tasha Fraser wrote: >> Does anyone have a good suggestion for a good HP control block or >> slides? >> >> >> >> Tasha Fraser, HT (ASCP) >> >> Olympic Medical Center >> >> Port Angeles, WA >> >> >> ------------------------------------------------------------------------- >> Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended individual(s) named above and may contain confidential, privileged, and/or protected information. Any unauthorized review, use, disclosure, copying, or distribution of its contents is prohibited. If you are not the intended recipient, you have received this email in error. If so, please notify the sender immediately by reply email and delete/destroy the original and all copies of this communication. Also know that Internet e-mail is not secure. In choosing to communicate with Olympic Medical Center by email you will assume these confidentiality risks. Internet messages may become corrupted, incomplete, or may incorrectly identify the sender. >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > -- > Hans B Snyder > Histologistics > 100 Barber Ave > Worcester, MA 01606 > 508-308-7800 > hans@histologistics.com > > > > -- > Hans B Snyder > Histologistics > 100 Barber Ave > Worcester, MA 01606 > 508-308-7800 > hans@histologistics.com From rsrichmond <@t> gmail.com Sat Jan 5 13:26:35 2013 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Sat Jan 5 13:26:41 2013 Subject: [Histonet] Re: Helicobacter stains and controls Message-ID: There are four broad choices for staining Helicobacter. 1. Rely on H & E alone. Some people claim it works, but I can't find them unless they're all over the place. 2. Thiazine dye methods. The simplest is the blue dye mix Diff-Quik II (or a generic equivalent, all of which work in my experience), the next a Giemsa stain, and it's possible to complicate them considerably more. 3. Silver stains such as Steiner's. They work, but they're a lot of trouble and the tissue often falls off the slide. I have almost no experience with them. 4. Immunostains. More expensive to do, but a lot faster for the pathologist to read. I haven't seen a study comparing the sensitivity of these methods. My personal opinion is that I'm OK with Diff-Quik II if I'm seeing one gastric biopsy a day, but I want IHC if I'm going to be seeing ten of them. With the dye method I often have to resort to oil immersion magnification, where with IHC I barely need to use a high-dry lens. **************************************** Helicobacter controls: Not really necessary, but should be done anyway. You need the co-operation of your pathologist in finding positives suitable for use as controls. One gastrectomy specimen can of course set you up for life, but these are pretty rare these days. Some additional points: many pathologists don't understand that not all bacteria they see in the stomach are Helicobacter - morphologic criteria must be observed. (IHC gets around this problem.) There is another, rather rare Helicobacter that causes peptic ulcer disease, with different morphology (a tight corkscrew rather than a "gull wing") - Helicobacter heilmanii. Supposedly IHC picks it up. There's controversy as to whether all gastric biopsy specimens need a stain of some kind, or only the ones with acute inflammation (confusingly called chronic active gastritis). Bob Richmond Samurai Pathologist Maryville TN From dunatrsd <@t> sbcglobal.net Sat Jan 5 13:36:38 2013 From: dunatrsd <@t> sbcglobal.net (dusko trajkovic) Date: Sat Jan 5 13:36:42 2013 Subject: [Histonet] Fw: PSCA Primary antibody In-Reply-To: <89F4666A496DC949A819ECC40E11C867BF56F481@EXCHANGEMB1.hmc.hurleymc.com> References: <5290DE49B23B6240BC0DF14FC8142D1901CB9083@is-210vs.olympicmedical.local>, <89F4666A496DC949A819ECC40E11C867BF56F481@EXCHANGEMB1.hmc.hurleymc.com> Message-ID: <1357414598.7119.YahooMailRC@web181705.mail.ne1.yahoo.com> Hallo Histonetters, Happy New Year!! I'm looking for?information on a PSCA (Prostate Specific Cell antigen) antibody that will work both in human and primate. If are are using this antibody, please contact me.?Any information would be appreciated. thanks Dusko Trajkovic 858-638-6202 From rsrichmond <@t> gmail.com Sat Jan 5 13:37:06 2013 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Sat Jan 5 13:37:11 2013 Subject: [Histonet] Re: Pathologists' Names on H&E Labels Message-ID: Pathologist's name on the slide? I'd just like to see the patient's name on the slide. It helps me not mix up cases, but the old-timer histotechnologists really resist it. I guess you can legal-beagle reasons not to put the pathologist's name on the slide, but I don't see anything wrong with it. Seems like initials would suffice. Bob Richmond Samurai Pathologist Maryville TN From rsrichmond <@t> gmail.com Sat Jan 5 13:46:17 2013 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Sat Jan 5 13:46:22 2013 Subject: [Histonet] Re: CAP guideline for fixation time for ER/PR/HER2 Message-ID: Time of fixation before ER/PR/HER2: I haven't had much luck with getting this information. Nobody wants to deal with the communication issues. Needle biopsy specimens should be popped into formalin as soon as they are out of the patient, and the time recorded. What you can't find out is whether they are willing to fix the specimens before they take the time to X-ray them. Attempts to find out what they're doing are futile. To the OR nurse, a specimen is perfectly fixed all the way through the instant she drops it into formalin, whether a small lumpectomy or a Dolly-sized mammoon. Eventually we're going to have to realize that larger specimens (more than needle biopsy size) require prompt dissection before fixation, if special studies are to be done. Bob Richmond Samurai Pathologist Maryville TN From dlschneider <@t> gmail.com Sat Jan 5 14:14:51 2013 From: dlschneider <@t> gmail.com (Daniel Schneider) Date: Sat Jan 5 14:14:56 2013 Subject: [Histonet] Re: Pathologists' Names on H&E Labels In-Reply-To: References: Message-ID: You can get Sharpies and highlighters in at least 7 different colors. We color code the pathologists (Dr's Red, Blue, Pink, Orange, Purple, Green, and Yellow.) The Sharpies and highlighters are distributed in the appropriate departments, and the slides and paperwork get a quick mark of color to facilitate their delivery to the appropriate pathologist. Dan Schneider Amarillo, TX On Sat, Jan 5, 2013 at 1:37 PM, Bob Richmond wrote: > Pathologist's name on the slide? I'd just like to see the patient's > name on the slide. It helps me not mix up cases, but the old-timer > histotechnologists really resist it. > > I guess you can legal-beagle reasons not to put the pathologist's name > on the slide, but I don't see anything wrong with it. Seems like > initials would suffice. > > Bob Richmond > Samurai Pathologist > Maryville TN > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From pruegg <@t> ihctech.net Sat Jan 5 14:37:34 2013 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sat Jan 5 14:37:37 2013 Subject: [Histonet] holly nuclei bat man Message-ID: <656BC72C65FC454CA03123D09FA0C6DB@DESKTOP3> Happy New Year everyone! Can we start a discussion once again on what causes holes in nuclei? It is pretty clear in my experience it has something to do with heating at least for me with antigen retrieval. When I do HIER at ph 8 especially I am seeing a lot of holes in nuclei. H&E on same tissue alone without HIER/IHC doesn't seem to exhibit this artifact (holes in the nuclei). I bet it can also happen if the tissue is not fixed well enough to protect it from paraffin processing but right now I am seeing it more after HIER, don't see it as much on my IHC slides with no AR or when I use eier instead of hier. Also notice that ph6 HIER and Ph9 HIER are not as bad as when I use Ph8 for the same times and temps. Cheers, Patsy Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting, LLC 40864 Arkansas Ave Bennett, CO 80102 Fax: 720-859-4110 pruegg@ihctech.net From gu.lang <@t> gmx.at Sun Jan 6 05:32:00 2013 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sun Jan 6 05:32:06 2013 Subject: AW: [Histonet] holly nuclei bat man In-Reply-To: <656BC72C65FC454CA03123D09FA0C6DB@DESKTOP3> References: <656BC72C65FC454CA03123D09FA0C6DB@DESKTOP3> Message-ID: <000901cdec01$7277f900$5767eb00$@gmx.at> I have a quiet semi-scientific explanation. Formalin-fixation stabilizes proteins via crosslinking. AR "solves" methylol-adducts due to formaldehyde and perhaps also some crosslinks. Nuclei also have a protein-scaffold, filled with nucleinacids. It seems like a "ladder" or "hole in tights". It seems like there is a kind of tension, after breaking a crosslink the hole "blubbs" out. This is also seen with pretreatment in hybridization-procedures. The more enzymatic and heat-retrieval the more holes. And there is a direct relationsship between fixation-quality and AR-strength. Bye Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Patsy Ruegg Gesendet: Samstag, 05. J?nner 2013 21:38 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] holly nuclei bat man Happy New Year everyone! Can we start a discussion once again on what causes holes in nuclei? It is pretty clear in my experience it has something to do with heating at least for me with antigen retrieval. When I do HIER at ph 8 especially I am seeing a lot of holes in nuclei. H&E on same tissue alone without HIER/IHC doesn't seem to exhibit this artifact (holes in the nuclei). I bet it can also happen if the tissue is not fixed well enough to protect it from paraffin processing but right now I am seeing it more after HIER, don't see it as much on my IHC slides with no AR or when I use eier instead of hier. Also notice that ph6 HIER and Ph9 HIER are not as bad as when I use Ph8 for the same times and temps. Cheers, Patsy Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting, LLC 40864 Arkansas Ave Bennett, CO 80102 Fax: 720-859-4110 pruegg@ihctech.net _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sabeti_shahram <@t> yahoo.com Sun Jan 6 09:07:46 2013 From: sabeti_shahram <@t> yahoo.com (Shahram Sabeti) Date: Sun Jan 6 09:07:50 2013 Subject: [Histonet] a problem with giemsa stain for h.pylori Message-ID: <1357484866.12642.YahooMailNeo@web120304.mail.ne1.yahoo.com> hello every body, ??? on giema staining of gastric tissue slides for h.pylori,we have encounterd a major problem for a while.the problem is deposition of large flakes on the mucosal surface and we do not know why? do you know how to get rid of this problem. thank you in advance for your kind help. kind regards, shahram. From rjbuesa <@t> yahoo.com Sun Jan 6 09:34:06 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sun Jan 6 09:34:12 2013 Subject: [Histonet] holly nuclei bat man In-Reply-To: <656BC72C65FC454CA03123D09FA0C6DB@DESKTOP3> References: <656BC72C65FC454CA03123D09FA0C6DB@DESKTOP3> Message-ID: <1357486446.63596.YahooMailNeo@web163103.mail.bf1.yahoo.com> According with some tests I made and an article published in the JOH, the so called "nuclear holes" occur when a section with some remaining amount of water underneath is dried in an oven at 60?C or more. The "explanation" is that the heated underlying water?"dissolves" nuclear material and hence the appearance of holes. I could look for the reference of the JOH article if you want. Ren? J. From: Patsy Ruegg To: histonet@lists.utsouthwestern.edu Sent: Saturday, January 5, 2013 3:37 PM Subject: [Histonet] holly nuclei bat man Happy New Year everyone! Can we start a discussion once again on what causes holes in nuclei?? It is pretty clear in my experience it has something to do with heating at least for me with antigen retrieval.? When I do HIER at ph 8 especially I am seeing a lot of holes in nuclei.? H&E on same tissue alone without HIER/IHC doesn't seem to exhibit this artifact (holes in the nuclei).? I bet it can also happen if the tissue is not fixed well enough to protect it from paraffin processing but right now I am seeing it more after HIER, don't see it as much on my IHC slides with no AR or when I use eier instead of hier. Also notice that ph6 HIER and Ph9 HIER are not as bad as when I use Ph8 for the same times and temps. Cheers, Patsy Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting, LLC 40864 Arkansas Ave Bennett, CO 80102 Fax: 720-859-4110 pruegg@ihctech.net _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Sun Jan 6 10:23:00 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sun Jan 6 10:23:04 2013 Subject: [Histonet] a problem with giemsa stain for h.pylori In-Reply-To: <1357484866.12642.YahooMailNeo@web120304.mail.ne1.yahoo.com> References: <1357484866.12642.YahooMailNeo@web120304.mail.ne1.yahoo.com> Message-ID: <1357489380.40031.YahooMailNeo@web163106.mail.bf1.yahoo.com> Filter your Giemsa solution before using it or better yet, get a newer staining solution Ren? J. From: Shahram Sabeti To: "histonet@lists.utsouthwestern.edu" Sent: Sunday, January 6, 2013 10:07 AM Subject: [Histonet] a problem with giemsa stain for h.pylori hello every body, ??? on giema staining of gastric tissue slides for h.pylori,we have encounterd a major problem for a while.the problem is deposition of large flakes on the mucosal surface and we do not know why? do you know how to get rid of this problem. thank you in advance for your kind help. kind regards, shahram. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Sun Jan 6 11:34:41 2013 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sun Jan 6 11:34:45 2013 Subject: [Histonet] holly nuclei bat man In-Reply-To: <000901cdec01$7277f900$5767eb00$@gmx.at> References: <656BC72C65FC454CA03123D09FA0C6DB@DESKTOP3> <000901cdec01$7277f900$5767eb00$@gmx.at> Message-ID: <79B650EE61904222AD8C5FCB2873489E@DESKTOP3> Yes this all makes good sense and explains why I may not be able to have control of this because I do not always do the tissue fixation and processing myself. That first step of adequately fixing (cross linking the proteins) to protect them from tissue processing and AR are sooooo important. The one thing I can control is to make sure the slides are completely dry before baking them as Renee mentioned. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting, LLC 40864 Arkansas Ave Bennett, CO 80102 Fax: 720-859-4110 pruegg@ihctech.net -----Original Message----- From: Gudrun Lang [mailto:gu.lang@gmx.at] Sent: Sunday, January 06, 2013 4:32 AM To: 'Patsy Ruegg' Cc: histonet@lists.utsouthwestern.edu Subject: AW: [Histonet] holly nuclei bat man I have a quiet semi-scientific explanation. Formalin-fixation stabilizes proteins via crosslinking. AR "solves" methylol-adducts due to formaldehyde and perhaps also some crosslinks. Nuclei also have a protein-scaffold, filled with nucleinacids. It seems like a "ladder" or "hole in tights". It seems like there is a kind of tension, after breaking a crosslink the hole "blubbs" out. This is also seen with pretreatment in hybridization-procedures. The more enzymatic and heat-retrieval the more holes. And there is a direct relationsship between fixation-quality and AR-strength. Bye Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Patsy Ruegg Gesendet: Samstag, 05. J?nner 2013 21:38 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] holly nuclei bat man Happy New Year everyone! Can we start a discussion once again on what causes holes in nuclei? It is pretty clear in my experience it has something to do with heating at least for me with antigen retrieval. When I do HIER at ph 8 especially I am seeing a lot of holes in nuclei. H&E on same tissue alone without HIER/IHC doesn't seem to exhibit this artifact (holes in the nuclei). I bet it can also happen if the tissue is not fixed well enough to protect it from paraffin processing but right now I am seeing it more after HIER, don't see it as much on my IHC slides with no AR or when I use eier instead of hier. Also notice that ph6 HIER and Ph9 HIER are not as bad as when I use Ph8 for the same times and temps. Cheers, Patsy Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting, LLC 40864 Arkansas Ave Bennett, CO 80102 Fax: 720-859-4110 pruegg@ihctech.net _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Sun Jan 6 11:57:55 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sun Jan 6 11:57:58 2013 Subject: [Histonet] holly nuclei bat man In-Reply-To: <79B650EE61904222AD8C5FCB2873489E@DESKTOP3> References: <656BC72C65FC454CA03123D09FA0C6DB@DESKTOP3> <000901cdec01$7277f900$5767eb00$@gmx.at> <79B650EE61904222AD8C5FCB2873489E@DESKTOP3> Message-ID: <1357495075.60977.YahooMailNeo@web163104.mail.bf1.yahoo.com> The reference for the "Nuclear bubbling" article is: Journal of Histotechnology, 1990; 13(2):135-6 Ren? J. From: Patsy Ruegg To: gu.lang@gmx.at Cc: histonet@lists.utsouthwestern.edu Sent: Sunday, January 6, 2013 12:34 PM Subject: RE: [Histonet] holly nuclei bat man Yes this all makes good sense and explains why I may not be able to have control of this because I do not always do the tissue fixation and processing myself.? That first step of adequately fixing (cross linking the proteins) to protect them from tissue processing and AR are sooooo important.? The one thing I can control is to make sure the slides are completely dry before baking them as Renee mentioned. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting, LLC 40864 Arkansas Ave Bennett, CO 80102 Fax: 720-859-4110 pruegg@ihctech.net -----Original Message----- From: Gudrun Lang [mailto:gu.lang@gmx.at] Sent: Sunday, January 06, 2013 4:32 AM To: 'Patsy Ruegg' Cc: histonet@lists.utsouthwestern.edu Subject: AW: [Histonet] holly nuclei bat man I have a quiet semi-scientific explanation. Formalin-fixation stabilizes proteins via crosslinking. AR "solves" methylol-adducts due to formaldehyde and perhaps also some crosslinks. Nuclei also have a protein-scaffold, filled with nucleinacids. It seems like a "ladder" or "hole in tights". It seems like there is a kind of tension, after breaking a crosslink the hole "blubbs" out. This is also seen with pretreatment in hybridization-procedures. The more enzymatic and heat-retrieval the more holes.? And there is a direct relationsship between fixation-quality and AR-strength. Bye Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Patsy Ruegg Gesendet: Samstag, 05. J?nner 2013 21:38 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] holly nuclei bat man Happy New Year everyone! Can we start a discussion once again on what causes holes in nuclei?? It is pretty clear in my experience it has something to do with heating at least for me with antigen retrieval.? When I do HIER at ph 8 especially I am seeing a lot of holes in nuclei.? H&E on same tissue alone without HIER/IHC doesn't seem to exhibit this artifact (holes in the nuclei).? I bet it can also happen if the tissue is not fixed well enough to protect it from paraffin processing but right now I am seeing it more after HIER, don't see it as much on my IHC slides with no AR or when I use eier instead of hier. Also notice that ph6 HIER and Ph9 HIER are not as bad as when I use Ph8 for the same times and temps. Cheers, Patsy Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting, LLC 40864 Arkansas Ave Bennett, CO 80102 Fax: 720-859-4110 pruegg@ihctech.net _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carl.hobbs <@t> kcl.ac.uk Sun Jan 6 12:44:10 2013 From: carl.hobbs <@t> kcl.ac.uk (Hobbs, Carl) Date: Sun Jan 6 12:44:21 2013 Subject: [Histonet] Re: holly nuclei bat man Message-ID: <00D6B8253EAED840B8D04E23549738181980E0FE@AM2PRD0311MB399.eurprd03.prod.outlook.com> Hmmm...I have raised this issue before....no answer. All answers to date....pure conjecture! Lol...if you meant "Holly".....rather than "Holy" or even "Holey"...nice one, Batwoman! Caped Crusader. From TMcNemar <@t> lmhealth.org Mon Jan 7 04:52:22 2013 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Mon Jan 7 04:52:02 2013 Subject: [Histonet] Pathologists' Names on H&E Labels In-Reply-To: <50E6FCA30200002300047B62@gwgwia2> References: <50E6FCA30200002300047B62@gwgwia2> Message-ID: We have recently moved to printed/barcoded labels for all of our slides. Each pathologist has a mnemonic in our LIS (Meditech) that we use when assigning the case. This mnemonic is picked up and printed on the slide label. It has been a real help, especially on recuts and stains. We no longer have to look up a specimen to see who it goes to. The mnemonic is just the pathologist's initials and I can see no reason why this should be a problem. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Roger Heyna Sent: Friday, January 04, 2013 5:01 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Pathologists' Names on H&E Labels For years, our lab's information system has been set up so that the name of the pathologist assigned to a case prints on our H&E labels to assist with sorting and grouping the slides after staining. As new pathologists join our team, concerns regarding this practice have come up. The pathologists are concerned that having their names on the slides pose medical-legal liability, and they want the names removed. I've yet to hear clear ramifications involved with having doctors' names on slides. What liability is really involved? We are a large, urban academic medical center with high specimen volumes. Our pathologists rotate through different sub-specialties, sometimes on a daily basis. For us, having the pathologists' names on the H&E labels seems to be the easiest way to get the H&E's to the correct pathologist. Are other labs printing the names of the pathologists on their H&E labels? Is anyone aware of any legal risk involved in this practice? Would anyone mind sharing how they sort and divide the slides before submitting them to their pathologists? Thank you, Roger Heyna, BS, HTL(ASCP) Loyola University Medical Center Maywood, IL This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From csheil <@t> jcu.edu Mon Jan 7 09:17:03 2013 From: csheil <@t> jcu.edu (Christopher Sheil) Date: Mon Jan 7 09:15:50 2013 Subject: [Histonet] staining site of bone deposition on live specimens Message-ID: Earlier I submitted a question to histonet to ask about labels that can be used to bind bone at the site of bone formation in aquatic amphibians. Robert Schoonhoven kindly sent a response stating that tetracycline has been used in mammalian systems. I also recently found a paper (citation below) that outlines the use of tetracycline, calcein, xylenol orange, and alizarine red S to identify Layers of Active Growth (LAG's) in a primarily aquatic frog. Citation: Francillon H and J Castanet. 1985. Experimental demonstration of the annual characteristic of the lines of arrest of skeletal growth in Rana esculenta (Amphibia, Anura). Comptes Rendus de L'Academie des Sciences, Serie III, Sciences de la vie. 300(8): 327?332. -Chris Christopher Sheil Graduate Coordinator Department of Biology John Carroll University 20700 North Park Boulevard University Heights, OH 44118 Email: csheil@jcu.edu Phone: 216.397.3088 From jim000001 <@t> hotmail.com Mon Jan 7 10:57:59 2013 From: jim000001 <@t> hotmail.com (Jim Jones) Date: Mon Jan 7 10:58:05 2013 Subject: [Histonet] TMA Cutting Problems Message-ID: We constructed some TMAs for Validations and when I tried to section the TMA blocks the sections shred. It's really hard to get a nice ribbon or even a nice section. I don't know what I am doing wrong. Has anyone had problems cutting TMAs before? Any solutions? We are using the Arraymold instrument. Jimmy From rjbuesa <@t> yahoo.com Mon Jan 7 11:09:36 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Jan 7 11:09:41 2013 Subject: [Histonet] TMA Cutting Problems In-Reply-To: References: Message-ID: <1357578576.20487.YahooMailNeo@web163103.mail.bf1.yahoo.com> Probably the paraffin wax surrounding the cores has not infiltrated them perfectly and you have ended with a TMA block that cannot hold the cores adequately. My suggestion is to melt the block again and leave the cores in melted paraffin during at least 1 hour and prepare new blocks that will ha able to hold together better. Ren? J. From: Jim Jones To: Histonet Sent: Monday, January 7, 2013 11:57 AM Subject: [Histonet] TMA Cutting Problems We constructed some TMAs for Validations and when I tried to section the TMA blocks the sections shred.? It's really hard to get a nice ribbon or even a nice section.? I don't know what I am doing wrong.? Has anyone had problems cutting TMAs before?? Any solutions?? We are using the Arraymold instrument. Jimmy ??? ??? ??? ? ??? ??? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DEBORAH_ELLENBURG <@t> bshsi.org Mon Jan 7 11:17:26 2013 From: DEBORAH_ELLENBURG <@t> bshsi.org (Ellenburg, Deborah) Date: Mon Jan 7 11:17:33 2013 Subject: [Histonet] CAP guideline for fixation time In-Reply-To: References: Message-ID: We have a label that we place on the original requisition documenting the time the specimen was placed in formalin and the time it came out of formalin(changed stations on tissue processor). When the transcriptionist transcribe the gross dictation they give the total number of hours the specimen was in formalin. The pathologist is able to see the total time in formalin when reviewing their working draft. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victor A. Tobias Sent: Friday, January 04, 2013 6:21 PM To: 'histonet' Subject: [Histonet] CAP guideline for fixation time Per CAP guideline, in our reports of predictive markers(ER/PR/Her2), Pathologists need to check that the total fixation time of specimens is within the CAP guideline. Is this information readily available to the pathologists at the time of writing the report? How are others documenting this? Have a great weekend. Victor Victor Tobias HT(ASCP) Clinical Applications Analyst Harborview Medical Center Dept of Pathology Room NJB244 Seattle, WA 98104 vtobias@u.washington.edu 206-744-2735 206-744-8240 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________________________________________________________________________________ The information in this communication is intended to be confidential to the Individual(s) and/or Entity to whom it is addressed. It may contain information of a Privileged and/or Confidential nature, which is subject to Federal and/or State privacy regulations. In the event that you are not the intended recipient or the agent of the intended recipient, do not copy or use the information contained within this communication, or allow it to be read, copied or utilized in any manner, by any other person(s). Should this communication be received in error, please notify the sender immediately either by response e-mail or by phone, and permanently delete the original e-mail, attachment(s), and any copies. ________________________________________________________________________________________________________ From jim000001 <@t> hotmail.com Mon Jan 7 11:39:41 2013 From: jim000001 <@t> hotmail.com (Jim Jones) Date: Mon Jan 7 11:39:45 2013 Subject: [Histonet] TMA Cutting Problems Message-ID: I just read on the Arraymold website that I am probably using the wrong type of paraffin for constructing my TMAs. I used Richard Allen Type 9 but they recommend Paraplast X-TRA from Leica or Blue Ribbon from Surgipath. I hope a different paraffin solves the issues. From cforster <@t> umn.edu Mon Jan 7 11:45:02 2013 From: cforster <@t> umn.edu (Colleen Forster) Date: Mon Jan 7 11:56:11 2013 Subject: [Histonet] EGFR primary Message-ID: <50EB099E.6020503@umn.edu> Hello everyone, Can you please share with me the EGFR primary antibody you use and what pretreatment you use with it....thanks in advance!! Colleen Forster From Joyce.Weems <@t> emoryhealthcare.org Mon Jan 7 11:59:52 2013 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Mon Jan 7 12:00:09 2013 Subject: [Histonet] RE: CAP guideline for fixation time In-Reply-To: References: Message-ID: Our OR and Breast Health staff have done pretty well getting on board with this. We rarely have one that hasn't been documented. We have a place on the requisition (located just under the date field so they will see it) where time out of body (for the cold ischemic time), and time into formalin is documented. Then we document the rest on the requisition and the pathologist takes it from there. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victor A. Tobias Sent: Friday, January 04, 2013 6:21 PM To: 'histonet' Subject: [Histonet] CAP guideline for fixation time Per CAP guideline, in our reports of predictive markers(ER/PR/Her2), Pathologists need to check that the total fixation time of specimens is within the CAP guideline. Is this information readily available to the pathologists at the time of writing the report? How are others documenting this? Have a great weekend. Victor Victor Tobias HT(ASCP) Clinical Applications Analyst Harborview Medical Center Dept of Pathology Room NJB244 Seattle, WA 98104 vtobias@u.washington.edu> 206-744-2735 206-744-8240 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From JEllin <@t> yumaregional.org Mon Jan 7 12:02:35 2013 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Mon Jan 7 12:02:46 2013 Subject: [Histonet] RE: CAP guideline for fixation time In-Reply-To: References: Message-ID: We have it documented within the APLIS, when the order is placed within our EMR,, for requisitions we have them document them on the requisition at the time it is taking place with a sticker,, but since the most of our orders are electronic it is part of the workflow, this is done for all specimens as we see the importance of other biomarkers for future -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Monday, January 07, 2013 11:00 AM To: 'Victor A. Tobias'; 'histonet' Subject: [Histonet] RE: CAP guideline for fixation time Our OR and Breast Health staff have done pretty well getting on board with this. We rarely have one that hasn't been documented. We have a place on the requisition (located just under the date field so they will see it) where time out of body (for the cold ischemic time), and time into formalin is documented. Then we document the rest on the requisition and the pathologist takes it from there. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victor A. Tobias Sent: Friday, January 04, 2013 6:21 PM To: 'histonet' Subject: [Histonet] CAP guideline for fixation time Per CAP guideline, in our reports of predictive markers(ER/PR/Her2), Pathologists need to check that the total fixation time of specimens is within the CAP guideline. Is this information readily available to the pathologists at the time of writing the report? How are others documenting this? Have a great weekend. Victor Victor Tobias HT(ASCP) Clinical Applications Analyst Harborview Medical Center Dept of Pathology Room NJB244 Seattle, WA 98104 vtobias@u.washington.edu> 206-744-2735 206-744-8240 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ From melissa <@t> alliedsearchpartners.com Mon Jan 7 12:06:06 2013 From: melissa <@t> alliedsearchpartners.com (Melissa Phelan) Date: Mon Jan 7 12:06:17 2013 Subject: [Histonet] Histology/Cytology Jobs in New York Message-ID: Allied Search Partners has the following positions available for permanent placement. Please contact me if you would like more details. Grossing Histotech Monday-Friday 9pm-5:30am or Monday-Friday 7pm-3:30am Westchester area *NY Clinical Lab or Histology License Required Cytoprep Technician Monday-Friday 10pm-6:30am Westchester area *NY Clinical Lab or Histology License Required Pathologist Assistant Monday-Friday 9pm-5:30am Westchester area *Bachelor?s Degree in Science Required. NY Clinical Lab or Histology license required. IHC Histotech Monday-Friday 1pm-9:30pm Westchester area *New York Clinical Lab or Histology License Required. IHC experience required. Histotech Monday-Friday 10am-6:30pm Long Island *New York Clinical Lab or Histology License Required. Histology experience required. Cytoprep Technician Monday-Friday 8am-4:30pm. W/ one Saturday per month. Long Island *Cytology Prep Experience required. NY Clinical Lab License required To view a complete list of Allied Search Partners current openings go to: http://www.alliedsearchpartners.com/careers.php Melissa Phelan LinkedIn: http://www.linkedin.com/in/melissaphelan President, Laboratory Staffing Allied Search Partners P: 888.388.7571 F: 888.388.7572 M: 407.697.1175 www.alliedsearchpartners.com From Sandra.Harrison3 <@t> va.gov Mon Jan 7 12:12:49 2013 From: Sandra.Harrison3 <@t> va.gov (Harrison, Sandra C.) Date: Mon Jan 7 12:13:21 2013 Subject: [Histonet] Leica 4020 linear stainer Message-ID: Could I get some feedback on linear stainers? Has anyone had any experience with the Leica ST4020? We are going to purchase a linear stainer for a new MOHS laboratory. Thanks, Sandy Harrison Histology Supervisor VA Health Care Systems, Minneapolis 612-467-2449 From amber.mckenzie <@t> gastrodocs.net Mon Jan 7 14:01:33 2013 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Mon Jan 7 14:01:38 2013 Subject: [Histonet] QC for fire shower In-Reply-To: References: <1046889651.1684067.1356725919030.JavaMail.root@sz0094a.emeryville.ca.mail.comcast.net> Message-ID: <5A33C952BB67F4468AF1F36D739212BC7B5D2323@JERRY.Gia.com> Does anyone have a QC log on the yearly fire shower testing they'd like to share? From Timothy.Morken <@t> ucsfmedctr.org Mon Jan 7 14:29:23 2013 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Mon Jan 7 14:29:30 2013 Subject: [Histonet] RE: QC for fire shower In-Reply-To: <5A33C952BB67F4468AF1F36D739212BC7B5D2323@JERRY.Gia.com> References: <1046889651.1684067.1356725919030.JavaMail.root@sz0094a.emeryville.ca.mail.comcast.net> <5A33C952BB67F4468AF1F36D739212BC7B5D2323@JERRY.Gia.com> Message-ID: <761E2B5697F795489C8710BCC72141FF04CF19@ex07.net.ucsf.edu> You mean chemical / fire shower? Our hospital has a guy test it once a month and record it. And record on a tag on the shower the date it was tested? Tim Morken UCSF PATHOLOGY -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Monday, January 07, 2013 12:02 PM To: . Subject: [Histonet] QC for fire shower Does anyone have a QC log on the yearly fire shower testing they'd like to share? From baazaouinarjes <@t> gmail.com Mon Jan 7 17:56:45 2013 From: baazaouinarjes <@t> gmail.com (Narjes) Date: Mon Jan 7 17:56:56 2013 Subject: [Histonet] Mouse eye histology Message-ID: <0233E6A7-CBFC-4A37-A730-BCA66ADB63BF@gmail.com> Hello, I need some help on how to remove the sucrose from mouse eye balls also I need a good protocol for fixation and sectioning of mouse eye balls (without detaching the retina from the rest of the eye section) for Immunohistochemical staining. Any help I would really appreciate it Thank you Sent from my iPad From JMacDonald <@t> mtsac.edu Mon Jan 7 18:57:34 2013 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Mon Jan 7 18:57:39 2013 Subject: [Histonet] Disposable blade holder Message-ID: Hi All, I am searching for a disposable blade holder for an older cryostat. It is a Heidelberg HM 500 (Microm). Thanks, Jennifer MacDonald Mt. San Antonio College From cbodden <@t> health.usf.edu Tue Jan 8 02:42:12 2013 From: cbodden <@t> health.usf.edu (Bodden, Cheryl) Date: Tue Jan 8 02:42:27 2013 Subject: [Histonet] Multi cassette bases Message-ID: I have a large surplus supply of Leica Multi Cassette Bases (catalog # 2238) in every color. I wanted to find out if anybody out there could use these. Cheryl Bodden University of South Florida Dermpath Lab From lpwenk <@t> sbcglobal.net Tue Jan 8 03:13:14 2013 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Tue Jan 8 03:13:17 2013 Subject: [Histonet] Multi cassette bases In-Reply-To: References: Message-ID: <83F7A14950F448179D508E147022C02A@HP2010> How about a HT or HTL program in Florida? Programs always need help with expenses. (For others who might have supplies or equipment to donate to HT/HTL programs, go to www.naacls.org --> on left, click on search, then click on HT or HTL and the state. If you are a non-profit hospital, get a letter from the program acknowledging the donation (eg., how many boxes of cassettes, and the cost). To remain non-profit, institutions must show that they are contributing to the community - health fairs, talks at clubs or schools, donation of supplies, etc. So submit the letter to whoever in your institution has to keep track of contributions.) HT Florida State College at Jacksonville North Campus 4501 Capper Road Jacksonville, FL 32202 Mr. Jerry Santiago MSEd, BS, HTL(ASCP)QIHC (904) 244-6129 HT Miami Dade College Medical Center Campus 950 NW 20th St Miami, FL 33127-4693 Ms. Caridad Ivis Gutierrez MAEd, BS, HTL(ASCP) (305) 237-4231 HT Keiser University 5600 Lake Underhill Rd. Orlando, FL 32807- Ms. Tracy Rosati MS, HT(ASCP) (407) 273-5800 HT Keiser University - Pembroke Pines 12520 Pines Boulevard Pembroke Pines, FL 33027- Ms. Galina Negrouk MS, HTL(ASCP) (954) 431-4300 ext. 164 HTL Barry University 11300 Northeast Second Avenue Miami Shores, FL 33161-6695 Dr. Gerhild Packert PhD (305) 899-3220 Peggy A. Wenk, HTL(ASCP) Beaumont Hospital Royal Oak, MI 48073 Lee & Peggy Wenk -----Original Message----- From: Bodden, Cheryl Sent: Tuesday, January 08, 2013 3:42 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Multi cassette bases I have a large surplus supply of Leica Multi Cassette Bases (catalog # 2238) in every color. I wanted to find out if anybody out there could use these. Cheryl Bodden University of South Florida Dermpath Lab _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 <@t> earthlink.net Tue Jan 8 09:41:59 2013 From: relia1 <@t> earthlink.net (Pam Barker) Date: Tue Jan 8 09:42:01 2013 Subject: [Histonet] RELIA Histology Careers Bulletin 01/08/2013 Happy New Year!! Message-ID: <00cc01cdedb6$b2f51790$18df46b0$@earthlink.net> Hi Histonetters!! Happy New Year!! I make a number of New Year?s Resolutions every year and the one that I find that I keep the most consistently and get the most satisfaction from is: My yearly resolution to help even more histo techs find the right opportunity in the right place at the right time. In 2012 I helped people make the move into a management role, relocate closer to family, shorten their commute, move into research, utilize their advanced degrees, get better salaries, shifts and benefits. In 2013 I resolve to help even more people make the changes they want to make in order to improve their career situations and ultimately their quality of life. RELIA?s Candidate Guarantee: The Right Job in the Right Place at the Right time FOR YOU! Remember, If you know someone who might be interested I also offer a $500 referral bonus for leads on candidates and job openings I have a nationwide network of contacts with the premier employers of histology professionals in clinical, research and biotech settings. I only work with full time permanent positions at companies that offer excellent compensation, benefits programs and relocation assistance. Here is a list of my current openings if I could I would spotlight all of them!!: ? Pathology Manager ? Fresno, CA- Great group of techs to manage!! Join their team!! ? Grossing Histotechnologist ? Charlotte, NC-Excellent benefits and proven stability!! ? Histotech HT/HTL ? Charlotte, NC multiple openings-Brand New Lab with a great team ? Histology Tech ? Nashville TN ? multiple openings-Outstanding team environment!! ? Lead Grossing Histotechnologist - Chattanooga, TN-Develop your leadership skills!! ? HT/HTL ? Philadelphia, PA Brand New Lab!!top notch team to work with!! ? ASCP or elig Histotech ? Augusta, GA Busy lab with a great team. ? Histotechnologist HT/HTL ? Florence S.C. Beautiful area and a great family atmosphere. ? Histotech ?Dayton, OH Innovative, Fast-Paced, growing lab. **I also have opportunities on Long Island for histotechs, IHC techs, FISH Techs and Molecular Techs. So if you or anyone you know has resolved to make a job change this year please let me know. We can get started right away or we can map out a strategy for when the timing is right. Shoot me an e-mail at relia1@earthlink.net or call me toll free at 866-607-3542. There are a lot of recruiters out there right now trying to work with histo techs and I appreciate your support and respect your needs. Remember I offer over 25 years of experience as a recruiter and for over 10 years I have dedicated my practice solely to placing histology professionals like you. Happy New Year and I hope to hear from you soon, Thanks-Pam Happy New Year!! Thank You! ? ? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From MMargiotta <@t> bmhmc.org Tue Jan 8 10:11:56 2013 From: MMargiotta <@t> bmhmc.org (Margiotta-Watz, Michele) Date: Tue Jan 8 10:12:03 2013 Subject: [Histonet] Pathology IS Message-ID: <230D0B9EC57D7A45A7A186C6AB4C7ABC296DC420@BMH-EXCHANGE-01.BMHMC.ORG> Hi All, We will be looking to change our pathology information system very soon. We currently use PowerPath/Tamtron. Any info regarding other systems out there that you might recommend would be greatly appreciated! Thanks, Michele Histology Supervisor BMHMC 631-654-7192 DISCLAIMER: This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. From jim000001 <@t> hotmail.com Tue Jan 8 10:48:56 2013 From: jim000001 <@t> hotmail.com (Jim Jones) Date: Tue Jan 8 10:49:01 2013 Subject: FW: [Histonet] TMA Cutting Problems In-Reply-To: References: , Message-ID: Paraplast X-TRA made a world of difference. We did a test TMA using our tissues which were processed with Richard Allen Type 9. The Arraymold was filled with Paraplast X-TRA. I put it in the oven at 40 C for 4 hours, chilled the TMA block and it cut beautifully. The process was much easier than I thought. I guess I should of watched the videos first... Thanks Hugh. Thanks for your help everyone. Jimmy From: hlukey@msn.com To: jim000001@hotmail.com Subject: RE: [Histonet] TMA Cutting Problems Date: Mon, 7 Jan 2013 12:20:18 -1000 Jimmy, I agree with Rene, as the "Section shreds" due to inconsistencies caused by type-9 paraffin polymers not lining up correctly. The difference in paraffins is demonstrated by it's physical differences in the paraffins, such as different melting points. If you were going to use it for TMA construction, you are going to want to "Temper" the block more thoroughly: More times, longer durations (ie heat overnight, refrigerator 4 hours, heat 2 hours, refrigerator 2 hours, heat 1 hour, cold 1 hour. Also remember to never freeze paraffin TMA blocks that cause the paraffin to crack (~-30C). Since you are new at doing this, I would follow the Arraymold directions (like you stated in another email). It should work as stated on their web site. We do TMA builds all the time with Paraplast X-tra without issue. Hugh UH Cancer Center Pathology Shared resource lab manager > ------------------------------ > Date: Mon, 7 Jan 2013 09:09:36 -0800 (PST) > From: Rene J Buesa > Subject: Re: [Histonet] TMA Cutting Problems > To: Jim Jones , Histonet > > Message-ID: > <1357578576.20487.YahooMailNeo@web163103.mail.bf1.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Probably the paraffin wax surrounding the cores has not infiltrated them perfectly and you have ended with a TMA block that cannot hold the cores adequately. > My suggestion is to melt the block again and leave the cores in melted paraffin during at least 1 hour and prepare new blocks that will ha able to hold together better. > Ren? J. > > From: Jim Jones > To: Histonet > Sent: Monday, January 7, 2013 11:57 AM > Subject: [Histonet] TMA Cutting Problems > > > We constructed some TMAs for Validations and when I tried to section the TMA blocks the sections shred. It's really hard to get a nice ribbon or even a nice section. I don't know what I am doing wrong. Has anyone had problems cutting TMAs before? Any solutions? We are using the Arraymold instrument. > Jimmy _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ From amber.mckenzie <@t> gastrodocs.net Tue Jan 8 11:18:09 2013 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Tue Jan 8 11:18:12 2013 Subject: [Histonet] Validating the Ventana Benchmark Special Stainer In-Reply-To: References: <1046889651.1684067.1356725919030.JavaMail.root@sz0094a.emeryville.ca.mail.comcast.net> Message-ID: <5A33C952BB67F4468AF1F36D739212BC7B5D2660@JERRY.Gia.com> Has anyone validated the new Ventana Benchmark Special Stainer? I was wondering what the standard slides count was? I would figure less than validating the immuno instruments, but wanted to check with everyone. Thanks! From jim000001 <@t> hotmail.com Tue Jan 8 12:01:40 2013 From: jim000001 <@t> hotmail.com (Jim Jones) Date: Tue Jan 8 12:01:45 2013 Subject: [Histonet] Validating the Ventana Benchmark Special Stainer In-Reply-To: <5A33C952BB67F4468AF1F36D739212BC7B5D2660@JERRY.Gia.com> References: <1046889651.1684067.1356725919030.JavaMail.root@sz0094a.emeryville.ca.mail.comcast.net>, , <5A33C952BB67F4468AF1F36D739212BC7B5D2660@JERRY.Gia.com> Message-ID: We are validating Leica stainers using TMAs. We are using 20 to 30 tissues per TMA instead of 20 to 30 slides per antibody. The cost savings is huge. I don't know if it is any different with the Ventana. Jimmy From: amber.mckenzie@gastrodocs.net To: histonet@lists.utsouthwestern.edu Date: Tue, 8 Jan 2013 17:18:09 +0000 Subject: [Histonet] Validating the Ventana Benchmark Special Stainer Has anyone validated the new Ventana Benchmark Special Stainer? I was wondering what the standard slides count was? I would figure less than validating the immuno instruments, but wanted to check with everyone. Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From GaleL <@t> unionhospital.org Tue Jan 8 12:02:04 2013 From: GaleL <@t> unionhospital.org (Gale Limron) Date: Tue Jan 8 12:02:10 2013 Subject: [Histonet] interview Message-ID: Hello, I just found out today I will be doing 2nd interviews for 3 candidates for a part time Histology position at our hospital on Friday of this week. These candidates are not histotechs but are willing to do online training and take ASCP board exam within 24 months. I would appreciate some help with what questions to ask. I did not attend the 1st interviews but these were done by our lab manager who does not know a lot about what we do I histology............. Thank you! Gale Limron CT,HT (ASCP) Histology Supervisor Union Hospital 659 Boulevard Dover, Ohio 44622 330-343-3311 ext 2562 This e-mail is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential or otherwise protected from disclosure. Dissemination, distribution or copying of this e-mail or the information herein by anyone other than the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, is prohibited. If you received this message in error, please delete without copying and kindly e-mail a reply to inform us of the mistake in delivery. From lharris <@t> samhealth.org Tue Jan 8 12:58:46 2013 From: lharris <@t> samhealth.org (Lori Harris) Date: Tue Jan 8 12:58:52 2013 Subject: [Histonet] RE: Pathology IS In-Reply-To: <230D0B9EC57D7A45A7A186C6AB4C7ABC296DC420@BMH-EXCHANGE-01.BMHMC.ORG> References: <230D0B9EC57D7A45A7A186C6AB4C7ABC296DC420@BMH-EXCHANGE-01.BMHMC.ORG> Message-ID: <450EDC37E404D142AF67D7314C954C8A3C51E1919B@SHSMAILVI01.int.samhealth.net> Don't choose EPIC/Beaker. It's a disaster. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Margiotta-Watz, Michele Sent: Tuesday, January 08, 2013 8:12 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Pathology IS Hi All, We will be looking to change our pathology information system very soon. We currently use PowerPath/Tamtron. Any info regarding other systems out there that you might recommend would be greatly appreciated! Thanks, Michele Histology Supervisor BMHMC 631-654-7192 DISCLAIMER: This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From rjbuesa <@t> yahoo.com Tue Jan 8 14:36:36 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jan 8 14:36:40 2013 Subject: [Histonet] interview In-Reply-To: References: Message-ID: <1357677396.4179.YahooMailNeo@web163104.mail.bf1.yahoo.com> Your issue is a tough one because your candidates are not histotechs so I have to assume that they do not know how to cut. In my case I NEVER interviewed anybody for my lab who did not know to cut because I always kept a selection of the worse blocks I could find and 20 of them would have to be cut by the candidates to be evaluated by me for section and H&E staining quality. My question is: why are you trying to hire somebody who does not know anything about histology? An "economics" issue? Trying to pay less for the "histotech" after training? Please remember that you always will "get what you pay for". If that is the case I am at a loss about what to ask them other than previous education level because it will always be more probable to being able to teach somebody with some level of education above and beyond high school. Otherwise you will be replicating what was done about 60 years ago when? pathologists used to "select" secretaries, janitorial service personnel or family members?or anybody they would want to train on the job and pay the least amount of money after training. So I would go with education level, specially something about chemistry and biology to find out if the candidates would be able to learn the chemistry behind tissue processing and special stains. Prepare a list of questions on these subjects and do not get "blinded by appearances" or "personality" that often have nothing to do with working ethics or learning ability. Ren? J. From: Gale Limron To: "histonet@lists.utsouthwestern.edu" Sent: Tuesday, January 8, 2013 1:02 PM Subject: [Histonet] interview Hello, I just found out today I will be doing 2nd interviews for 3 candidates for a part time Histology position at our hospital on Friday of this week. These candidates are not histotechs but are willing to do online training and take ASCP board exam within 24 months. I would appreciate some help with what questions to ask. I did not attend the 1st interviews but these were done by our lab manager who does not know a lot about what we do I histology............. Thank you! Gale Limron CT,HT (ASCP) Histology Supervisor Union Hospital 659 Boulevard Dover, Ohio 44622 330-343-3311 ext 2562 This e-mail is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential or otherwise protected from disclosure. Dissemination, distribution or copying of this e-mail or the information herein by anyone other than the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, is prohibited. If you received this message in error, please delete without copying and kindly e-mail a reply to inform us of the mistake in delivery._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From becky.garrison <@t> jax.ufl.edu Tue Jan 8 15:03:42 2013 From: becky.garrison <@t> jax.ufl.edu (Garrison, Becky) Date: Tue Jan 8 15:03:47 2013 Subject: [Histonet] RE: Pathology IS In-Reply-To: <450EDC37E404D142AF67D7314C954C8A3C51E1919B@SHSMAILVI01.int.samhealth.net> References: <230D0B9EC57D7A45A7A186C6AB4C7ABC296DC420@BMH-EXCHANGE-01.BMHMC.ORG> <450EDC37E404D142AF67D7314C954C8A3C51E1919B@SHSMAILVI01.int.samhealth.net> Message-ID: <9E47DE9D490DCC42A2EAE94F22BF93F240B4EFDA@JX1B-MAIL1.umc.ufl.edu> Is there an EPIC/Beaker Pathology product?? Thought they had Lab module only. Our hospital LIS is EPIC. Hear that we will be going to the Lab product in the future. Would really like some input on them. Becky Garrison Pathology Supervisor Shands Jacksonville Jacksonville, FL 32209 904-244-6237, phone 904-244-4290, fax 904-393-3194, pager -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lori Harris Sent: Tuesday, January 08, 2013 1:59 PM To: Margiotta-Watz, Michele; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Pathology IS Don't choose EPIC/Beaker. It's a disaster. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Margiotta-Watz, Michele Sent: Tuesday, January 08, 2013 8:12 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Pathology IS Hi All, We will be looking to change our pathology information system very soon. We currently use PowerPath/Tamtron. Any info regarding other systems out there that you might recommend would be greatly appreciated! Thanks, Michele Histology Supervisor BMHMC 631-654-7192 DISCLAIMER: This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kkienitz <@t> orclinic.com Tue Jan 8 15:10:37 2013 From: kkienitz <@t> orclinic.com (Kienitz, Kari) Date: Tue Jan 8 15:11:36 2013 Subject: [Histonet] RE: interview In-Reply-To: References: Message-ID: <41400FFE517878449D89114DD2526090087EA15BD2@tocmail1.tocad.orclinic.com> Hi Gale, In my career I have had the pleasure of hiring many OJT histotechs. With varying degrees of education and a whole range of personalities. The things that tend to really stand out in the candidates who excelled are the ones who could follow direction, pay close attention to detail, and communicate well. Histology is a field where as many mundane tasks must be done as the complicated ones. For many years we would administer to all final candidates a simple aptitude test. It was honestly something most 3rd graders could do. Its was amazing how many people would get caught up following directions on where to place their name on the paper and simple math. Kari Kienitz HT, (ASCP) Histology Laboratory Portland Gastroenterology The Oregon Clinic 1111 NE 99th Ave Portland, OR 97220 503.935.8311 kkienitz@orclinic.com CONFIDENTIALITY WARNING: This e-mail and any attachments are for the exclusive and confidential use of the intended recipient. If you are not the intended recipient, please do not read, distribute or take action in reliance upon this missive. If you have received this in error, please notify the sender immediately by reply e-mail and delete this message and its attachments from your computer system. Thank you ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gale Limron [GaleL@unionhospital.org] Sent: Tuesday, January 08, 2013 10:02 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] interview Hello, I just found out today I will be doing 2nd interviews for 3 candidates for a part time Histology position at our hospital on Friday of this week. These candidates are not histotechs but are willing to do online training and take ASCP board exam within 24 months. I would appreciate some help with what questions to ask. I did not attend the 1st interviews but these were done by our lab manager who does not know a lot about what we do I histology............. Thank you! Gale Limron CT,HT (ASCP) Histology Supervisor Union Hospital 659 Boulevard Dover, Ohio 44622 330-343-3311 ext 2562 This e-mail is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential or otherwise protected from disclosure. Dissemination, distribution or copying of this e-mail or the information herein by anyone other than the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, is prohibited. If you received this message in error, please delete without copying and kindly e-mail a reply to inform us of the mistake in delivery._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jaylundgren <@t> gmail.com Tue Jan 8 16:16:31 2013 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Tue Jan 8 16:16:35 2013 Subject: [Histonet] Helicobater Pylori QC block and/or slides In-Reply-To: <688D207C-281C-4008-A90C-B031BCB63C66@histologistics.com> References: <5290DE49B23B6240BC0DF14FC8142D1901CB9083@is-210vs.olympicmedical.local> <688D207C-281C-4008-A90C-B031BCB63C66@histologistics.com> Message-ID: Nice. On Fri, Jan 4, 2013 at 10:21 PM, Hans B Snyder wrote: > We are a private histology lab that is willing to share. If you need > slides or blocks let us know! > > thanks > > Sent from my iPad > > On Jan 4, 2013, at 8:06 PM, Hans B Snyder wrote: > > > > > > > ---------- Forwarded message ---------- > > From: Hans B Snyder > > Date: Fri, Jan 4, 2013 at 5:32 PM > > Subject: Re: [Histonet] Helicobater Pylori QC block and/or slides > > To: Tasha Fraser > > > > > > We have a human colon tissue that is positive. > > > > > > On Fri, Jan 4, 2013 at 12:37 PM, Tasha Fraser < > tfraser@olympicmedical.org> wrote: > >> Does anyone have a good suggestion for a good HP control block or > >> slides? > >> > >> > >> > >> Tasha Fraser, HT (ASCP) > >> > >> Olympic Medical Center > >> > >> Port Angeles, WA > >> > >> > >> > ------------------------------------------------------------------------- > >> Confidentiality Notice: This e-mail message, including any > attachments, is for the sole use of the intended individual(s) named above > and may contain confidential, privileged, and/or protected information. > Any unauthorized review, use, disclosure, copying, or distribution of its > contents is prohibited. If you are not the intended recipient, you have > received this email in error. If so, please notify the sender immediately > by reply email and delete/destroy the original and all copies of this > communication. Also know that Internet e-mail is not secure. In choosing > to communicate with Olympic Medical Center by email you will assume these > confidentiality risks. Internet messages may become corrupted, > incomplete, or may incorrectly identify the sender. > >> _______________________________________________ > >> Histonet mailing list > >> Histonet@lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > -- > > Hans B Snyder > > Histologistics > > 100 Barber Ave > > Worcester, MA 01606 > > 508-308-7800 > > hans@histologistics.com > > > > > > > > -- > > Hans B Snyder > > Histologistics > > 100 Barber Ave > > Worcester, MA 01606 > > 508-308-7800 > > hans@histologistics.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From wdesalvo.cac <@t> outlook.com Tue Jan 8 18:04:38 2013 From: wdesalvo.cac <@t> outlook.com (WILLIAM DESALVO) Date: Tue Jan 8 18:04:44 2013 Subject: [Histonet] interview In-Reply-To: References: Message-ID: It is difficult to interview individuals that do not have any experience in the detailed and manual technology of the Histology lab, but you can find the right individuals to become exceptional Histotechnologists. Although it can be very time consuming to train individuals, the right individual worthy of all the effort must have the right attitude! If the individuals have the necessary science background to understand the complex processes used in Histotechnology and have the right attitude, then lack of aptitude can be overcome. I have been involved, for several years, in training individuals with no Histology experienced and have been always rewarded by the performance of the individuals that wanted to learn and become more than a embedding or microtomy techie. Histotechnology is so much more than slapping tissue into a mold or hacking paraffin sections off a block. Everything done in the Histology lab is directly tied to a patient outcome and quality and precision area must. Make sure the individuals understand how difficult it will be to gain the knowledge and experience necessary to pass the ASCP exam, while only working part-time for 24 months. Find out what type of commitment they are willing to make to better Histotechnology, the patient outcome and themselves before you and your team invest any effort. Above all, the right attitude is the big first step in becoming a competent Histotechnologist that is dedicated and compassionate about improving the patient experience. William DeSalvo, B.S., HTL(ASCP) Production Manager-Anatomic Pathology Chair, NSH Quality Management Committee Owner/Consultant, Collaborative Advantage Consulting > From: GaleL@unionhospital.org > To: histonet@lists.utsouthwestern.edu > Date: Tue, 8 Jan 2013 13:02:04 -0500 > Subject: [Histonet] interview > > Hello, > I just found out today I will be doing 2nd interviews for 3 candidates for a part time Histology position at our hospital on Friday of this week. These candidates are not histotechs but are willing to do online training and take ASCP board exam within 24 months. I would appreciate some help with what questions to ask. I did not attend the 1st interviews but these were done by our lab manager who does not know a lot about what we do I histology............. > Thank you! > > Gale Limron CT,HT (ASCP) > Histology Supervisor > Union Hospital > 659 Boulevard > Dover, Ohio 44622 > 330-343-3311 ext 2562 > > > > This e-mail is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential or otherwise protected from disclosure. Dissemination, distribution or copying of this e-mail or the information herein by anyone other than the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, is prohibited. If you received this message in error, please delete without copying and kindly e-mail a reply to inform us of the mistake in delivery._______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ajohnston <@t> oregonmed.net Tue Jan 8 18:07:09 2013 From: ajohnston <@t> oregonmed.net (Johnston, Amy) Date: Tue Jan 8 18:07:19 2013 Subject: [Histonet] Control Tissue Message-ID: <8F6C0286892B2544B10E5F31F1C7075D27B815CF@IPSPRDMBOX1.oregonmed.net> Is there any way to obtain control blocks? We are in the process of starting up a new histology lab and control slides are a lot more expensive than I thought:( . I have purchased several boxes already, I was hoping I could find the others as they should be easy to find. (I tried NSH, they no longer have a bank of blocks.) I still need controls for trichrome, iron and mucin, any suggestions? Amy Johnston HT(ASCP) Histology Technician Oregon Medical Group 4140 Quest Drive Eugene, OR 97402 541-463-2163 From kiran_g <@t> sbcglobal.net Tue Jan 8 18:26:55 2013 From: kiran_g <@t> sbcglobal.net (Kiranjit Grewal) Date: Tue Jan 8 18:27:00 2013 Subject: [Histonet] Histology growth/volume ?? In-Reply-To: <50E6E50F.7400.0077.0@harthosp.org> Message-ID: <1357691215.54694.YahooMailClassic@web184404.mail.bf1.yahoo.com> Dear Histonetters, ? I need you help answering some of questions being asked by our bussiness leaders in regards to Histology growth independent of membership growth. ? What is the national literature saying about Histology testing volumes? Or, what is the medical field expecting in terms of histology growth? more biopies or blocks? ? Any ideas? or pathology data that can help. ? Thank you in advance, ? Kiran Grewal. ? From ree3 <@t> leicester.ac.uk Wed Jan 9 02:50:43 2013 From: ree3 <@t> leicester.ac.uk (Edwards, Richard E.) Date: Wed Jan 9 02:50:53 2013 Subject: [Histonet] interview In-Reply-To: References: Message-ID: <7722595275A4DD4FA225B92CDBF174A101A7F64680DE@EXC-MBX3.cfs.le.ac.uk> Well if you only pay peanuts you only get monkeys...................... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO Sent: 09 January 2013 00:05 To: Gale Limron; histonet Subject: RE: [Histonet] interview It is difficult to interview individuals that do not have any experience in the detailed and manual technology of the Histology lab, but you can find the right individuals to become exceptional Histotechnologists. Although it can be very time consuming to train individuals, the right individual worthy of all the effort must have the right attitude! If the individuals have the necessary science background to understand the complex processes used in Histotechnology and have the right attitude, then lack of aptitude can be overcome. I have been involved, for several years, in training individuals with no Histology experienced and have been always rewarded by the performance of the individuals that wanted to learn and become more than a embedding or microtomy techie. Histotechnology is so much more than slapping tissue into a mold or hacking paraffin sections off a block. Everything done in the Histology lab is directly tied to a patient outcome and quality and precision area must. Make sure the individuals understand how difficult it will be to gain the knowledge and experience necessary to pass the ASCP exam, while only working part-time for 24 months. Find out what type of commitment they are willing to make to better Histotechnology, the patient outcome and themselves before you and your team invest any effort. Above all, the right attitude is the big first step in becoming a competent Histotechnologist that is dedicated and compassionate about improving the patient experience. William DeSalvo, B.S., HTL(ASCP) Production Manager-Anatomic Pathology Chair, NSH Quality Management Committee Owner/Consultant, Collaborative Advantage Consulting > From: GaleL@unionhospital.org > To: histonet@lists.utsouthwestern.edu > Date: Tue, 8 Jan 2013 13:02:04 -0500 > Subject: [Histonet] interview > > Hello, > I just found out today I will be doing 2nd interviews for 3 candidates for a part time Histology position at our hospital on Friday of this week. These candidates are not histotechs but are willing to do online training and take ASCP board exam within 24 months. I would appreciate some help with what questions to ask. I did not attend the 1st interviews but these were done by our lab manager who does not know a lot about what we do I histology............. > Thank you! > > Gale Limron CT,HT (ASCP) > Histology Supervisor > Union Hospital > 659 Boulevard > Dover, Ohio 44622 > 330-343-3311 ext 2562 > > > > This e-mail is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential or otherwise protected from disclosure. Dissemination, distribution or copying of this e-mail or the information herein by anyone other than the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, is prohibited. If you received this message in error, please delete without copying and kindly e-mail a reply to inform us of the mistake in delivery._______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 <@t> earthlink.net Wed Jan 9 07:08:33 2013 From: relia1 <@t> earthlink.net (Pam Barker) Date: Wed Jan 9 07:08:36 2013 Subject: [Histonet] RELIA Special Job Alert - Exciting opportunity in Philadelphia for a Molecular Diagnostic Tech 01/09/2013 Message-ID: <00a101cdee6a$6d9e7e20$48db7a60$@earthlink.net> Hi Histonetters!! I hope everybody is having a great day. I have a new position to tell you about that I am pretty excited about. If you are interested please let me know and if you know someone who is interested please pass the info along. Remember if I hire someone you refer you get a referral fee!! Here is the info: Molecular Diagnostic Tech Brand New Lab - Philadelphia A growing new lab located in the Philadelphia area is hiring a Molecular Diagnostic Tech for a full time position and has asked RELIA Solutions to assist in the search. ASCP or eligible, BS degree in Medical Technology or Cytology and at least 1 year of FISH experience is required. This is a full time permanent position and my client offers an excellent salary, great benefits and an outstanding team to work with. They are ready to interview and hire now!! If this is the right job for you RELIA can make it happen! For more information please contact Pam Barker at relia1@earthlink.net or toll free at 866-607-3542 RELIA Solutions is the nation's ONLY recruiting firm specializing in the nationwide permanent placement of histology professionals. To sign up for our free histology careers bulletin please send an email to relia1@earthlink.net and include subscribe in the subject line. Happy New Year!! Thank You! Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solution Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From brett_connolly <@t> merck.com Wed Jan 9 07:53:37 2013 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Wed Jan 9 07:53:42 2013 Subject: [Histonet] RE: Control Tissue In-Reply-To: <8F6C0286892B2544B10E5F31F1C7075D27B815CF@IPSPRDMBOX1.oregonmed.net> References: <8F6C0286892B2544B10E5F31F1C7075D27B815CF@IPSPRDMBOX1.oregonmed.net> Message-ID: Amy - here are a few... Cooperative Human Tissue Network- http://www.chtn.nci.nih.gov/ Asterand biorepository. - https://www.asterand.com/Asterand/ NDRI - http://ndriresource.org/ Analytical Biological Services, Inc. - http://www.absbioreagents.com/products/products.html Brett M. Connolly, Ph.D. Principal Scientist, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Johnston, Amy Sent: Tuesday, January 08, 2013 7:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Control Tissue Is there any way to obtain control blocks? We are in the process of starting up a new histology lab and control slides are a lot more expensive than I thought:( . I have purchased several boxes already, I was hoping I could find the others as they should be easy to find. (I tried NSH, they no longer have a bank of blocks.) I still need controls for trichrome, iron and mucin, any suggestions? Amy Johnston HT(ASCP) Histology Technician Oregon Medical Group 4140 Quest Drive Eugene, OR 97402 541-463-2163 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From rjbuesa <@t> yahoo.com Wed Jan 9 08:48:27 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jan 9 08:48:31 2013 Subject: [Histonet] Control Tissue In-Reply-To: <8F6C0286892B2544B10E5F31F1C7075D27B815CF@IPSPRDMBOX1.oregonmed.net> References: <8F6C0286892B2544B10E5F31F1C7075D27B815CF@IPSPRDMBOX1.oregonmed.net> Message-ID: <1357742907.28119.YahooMailNeo@web163105.mail.bf1.yahoo.com> At the start of your lab activities you will have to rely on colleagues from other labs willing to give you old blocks (those not required to be kept any longer) that are positive for some procedures. With time you will build your own "collection" from?your cases.? Try always to use tissues that are (+) for several procedures. Small intestine is good for mucin and trichrome. Bone marrow is good for Perl's method. Ren? J.? From: "Johnston, Amy" To: "histonet@lists.utsouthwestern.edu" Sent: Tuesday, January 8, 2013 7:07 PM Subject: [Histonet] Control Tissue Is there any way to obtain control blocks? We are in the process of starting up a new histology lab and control slides are a lot more expensive than I thought:( .? I have purchased several boxes already, I was hoping I could find the others as they should be easy to find.? (I tried NSH, they no longer have a bank of blocks.)? I still need controls for trichrome, iron and mucin, any suggestions? Amy Johnston HT(ASCP) Histology Technician Oregon Medical Group 4140 Quest Drive Eugene, OR 97402 541-463-2163 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Jan 9 08:55:24 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jan 9 08:55:28 2013 Subject: [Histonet] Histology growth/volume ?? In-Reply-To: <1357691215.54694.YahooMailClassic@web184404.mail.bf1.yahoo.com> References: <50E6E50F.7400.0077.0@harthosp.org> <1357691215.54694.YahooMailClassic@web184404.mail.bf1.yahoo.com> Message-ID: <1357743324.50734.YahooMailNeo@web163106.mail.bf1.yahoo.com> Check "CAP Today". This is a monthly publication from CAP that deals with this type of issues. Try to contact them with your questions. Ren? J. From: Kiranjit Grewal To: "Histonet@lists.utsouthwestern.edu" ; Richard Cartun ; wdesalvo.cac@outlook.com Sent: Tuesday, January 8, 2013 7:26 PM Subject: Re: [Histonet] Histology growth/volume ?? Dear Histonetters, ? I need you help answering some of questions being asked by our bussiness leaders in regards to Histology growth independent of membership growth. ? What is the national literature saying about Histology testing volumes? Or, what is the medical field expecting in terms of histology growth? more biopies or blocks? ? Any ideas? or pathology data that can help. ? Thank you in advance, ? Kiran Grewal. ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lcolbert <@t> pathmdlabs.com Wed Jan 9 09:55:49 2013 From: lcolbert <@t> pathmdlabs.com (Laurie Colbert) Date: Wed Jan 9 10:01:01 2013 Subject: [Histonet] Microtome blades Message-ID: <12ECD7346266D74691EC2BFC75285E450114BA28@BFL323E10.pathmdlabs.local> Does anyone know who I can purchase Accu-edge blades from, other than Cardinal or American Master Tech? Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab From b-frederick <@t> northwestern.edu Wed Jan 9 10:01:45 2013 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Wed Jan 9 10:01:49 2013 Subject: [Histonet] RE: Microtome blades In-Reply-To: <12ECD7346266D74691EC2BFC75285E450114BA28@BFL323E10.pathmdlabs.local> References: <12ECD7346266D74691EC2BFC75285E450114BA28@BFL323E10.pathmdlabs.local> Message-ID: <62C639732D3F274DACED033EBDF6ADAF2036281A@evcspmbx1.ads.northwestern.edu> VWR Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Wednesday, January 09, 2013 9:56 AM To: Histonet Post (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Microtome blades Does anyone know who I can purchase Accu-edge blades from, other than Cardinal or American Master Tech? Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DKBoyd <@t> chs.net Wed Jan 9 10:02:28 2013 From: DKBoyd <@t> chs.net (Boyd, Debbie M) Date: Wed Jan 9 10:02:36 2013 Subject: [Histonet] RE: Microtome blades In-Reply-To: <12ECD7346266D74691EC2BFC75285E450114BA28@BFL323E10.pathmdlabs.local> References: <12ECD7346266D74691EC2BFC75285E450114BA28@BFL323E10.pathmdlabs.local> Message-ID: <7EAFE982E328304DA6CE2B677BB76246794AD2A6@TN001WEXMBX12.US.chs.net> Mercedes Medical -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Wednesday, January 09, 2013 10:56 AM To: Histonet Post (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Microtome blades Does anyone know who I can purchase Accu-edge blades from, other than Cardinal or American Master Tech? Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From liz <@t> premierlab.com Wed Jan 9 10:13:55 2013 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Wed Jan 9 10:14:00 2013 Subject: [Histonet] RE: Microtome blades In-Reply-To: <12ECD7346266D74691EC2BFC75285E450114BA28@BFL323E10.pathmdlabs.local> Message-ID: <14E2C6176416974295479C64A11CB9AE016411ABF729@SBS2K8.premierlab.local> Mercedes Medical or directly from Sakura Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Wednesday, January 09, 2013 8:56 AM To: Histonet Post (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Microtome blades Does anyone know who I can purchase Accu-edge blades from, other than Cardinal or American Master Tech? Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Jan 9 11:14:46 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jan 9 11:14:52 2013 Subject: [Histonet] Microtome blades In-Reply-To: <12ECD7346266D74691EC2BFC75285E450114BA28@BFL323E10.pathmdlabs.local> References: <12ECD7346266D74691EC2BFC75285E450114BA28@BFL323E10.pathmdlabs.local> Message-ID: <1357751686.88572.YahooMailNeo@web163103.mail.bf1.yahoo.com> Sakura Ren? J. From: Laurie Colbert To: "Histonet Post (histonet@lists.utsouthwestern.edu)" Sent: Wednesday, January 9, 2013 10:55 AM Subject: [Histonet] Microtome blades Does anyone know who I can purchase Accu-edge blades from, other than Cardinal or American Master Tech? Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA? 90048 (323) 648-3214 direct (424) 245-7284 main lab _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Wed Jan 9 11:20:08 2013 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/OID/NCEZID)) Date: Wed Jan 9 11:20:22 2013 Subject: [Histonet] Microtome blades In-Reply-To: <1357751686.88572.YahooMailNeo@web163103.mail.bf1.yahoo.com> References: <12ECD7346266D74691EC2BFC75285E450114BA28@BFL323E10.pathmdlabs.local> <1357751686.88572.YahooMailNeo@web163103.mail.bf1.yahoo.com> Message-ID: If you order them from Cardinal they get them from Sakura....I think. Or maybe vice-versa....... Jeanine H. Bartlett Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 404-639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, January 09, 2013 12:15 PM To: Laurie Colbert; Histonet Post (histonet@lists.utsouthwestern.edu) Subject: Re: [Histonet] Microtome blades Sakura Ren? J. From: Laurie Colbert To: "Histonet Post (histonet@lists.utsouthwestern.edu)" Sent: Wednesday, January 9, 2013 10:55 AM Subject: [Histonet] Microtome blades Does anyone know who I can purchase Accu-edge blades from, other than Cardinal or American Master Tech? Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA? 90048 (323) 648-3214 direct (424) 245-7284 main lab _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From madeleinehuey <@t> gmail.com Wed Jan 9 11:29:38 2013 From: madeleinehuey <@t> gmail.com (Madeleine Huey) Date: Wed Jan 9 11:29:42 2013 Subject: [Histonet] Re: Histonet Digest, Vol 110, Issue 9 In-Reply-To: <50eda5c0.448db60a.1aa5.2a45SMTPIN_ADDED_MISSING@mx.google.com> References: <50eda5c0.448db60a.1aa5.2a45SMTPIN_ADDED_MISSING@mx.google.com> Message-ID: Accu-edge microtome blades from Fisher Scientific. madeleine_h@elcaminohospital.org > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert > Sent: Wednesday, January 09, 2013 9:56 AM > To: Histonet Post (histonet@lists.utsouthwestern.edu) > Subject: [Histonet] Microtome blades > > Does anyone know who I can purchase Accu-edge blades from, other than Cardinal or American Master Tech? > > Laurie Colbert, HT (ASCP) > Histology Supervisor > PATH MD > 8158 Beverly Blvd. > Los Angeles, CA 90048 > (323) 648-3214 direct > (424) 245-7284 main lab > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 18 > Date: Wed, 9 Jan 2013 16:02:28 +0000 > From: "Boyd, Debbie M" > Subject: [Histonet] RE: Microtome blades > To: Laurie Colbert , "Histonet Post > (histonet@lists.utsouthwestern.edu)" > > Message-ID: > <7EAFE982E328304DA6CE2B677BB76246794AD2A6@TN001WEXMBX12.US.chs.net> > Content-Type: text/plain; charset="us-ascii" > > Mercedes Medical > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert > Sent: Wednesday, January 09, 2013 10:56 AM > To: Histonet Post (histonet@lists.utsouthwestern.edu) > Subject: [Histonet] Microtome blades > > Does anyone know who I can purchase Accu-edge blades from, other than Cardinal or American Master Tech? > > Laurie Colbert, HT (ASCP) > Histology Supervisor > PATH MD > 8158 Beverly Blvd. > Los Angeles, CA 90048 > (323) 648-3214 direct > (424) 245-7284 main lab > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -------------------------------------------------------------------------- > Disclaimer: This electronic message may contain information that is > Proprietary, Confidential, or legally privileged or protected. It > is intended only for the use of the individual(s) and entity named > in the message. If you are not an intended recipient of this > message, please notify the sender immediately and delete the > material from your computer. Do not deliver, distribute or copy > this message and do not disclose its contents or take any action in > reliance on the information it contains. > > > > ------------------------------ > > Message: 19 > Date: Wed, 9 Jan 2013 09:13:55 -0700 > From: Elizabeth Chlipala > Subject: [Histonet] RE: Microtome blades > To: 'Laurie Colbert' , "Histonet Post > (histonet@lists.utsouthwestern.edu)" > > Message-ID: > <14E2C6176416974295479C64A11CB9AE016411ABF729@SBS2K8.premierlab.local> > Content-Type: text/plain; charset="us-ascii" > > Mercedes Medical or directly from Sakura > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > Manager > Premier Laboratory, LLC > PO Box 18592 > Boulder, CO 80308-1592 > (303) 682-3949 office > (303) 682-9060 fax > (303) 881-0763 cell > www.premierlab.com > > Ship to address: > > 1567 Skyway Drive, Unit E > Longmont, CO 80504 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert > Sent: Wednesday, January 09, 2013 8:56 AM > To: Histonet Post (histonet@lists.utsouthwestern.edu) > Subject: [Histonet] Microtome blades > > Does anyone know who I can purchase Accu-edge blades from, other than Cardinal or American Master Tech? > > Laurie Colbert, HT (ASCP) > Histology Supervisor > PATH MD > 8158 Beverly Blvd. > Los Angeles, CA 90048 > (323) 648-3214 direct > (424) 245-7284 main lab > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 20 > Date: Wed, 9 Jan 2013 09:14:46 -0800 (PST) > From: Rene J Buesa > Subject: Re: [Histonet] Microtome blades > To: Laurie Colbert , "Histonet Post > \(histonet@lists.utsouthwestern.edu\)" > > Message-ID: > <1357751686.88572.YahooMailNeo@web163103.mail.bf1.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Sakura > Ren? J. > > From: Laurie Colbert > To: "Histonet Post (histonet@lists.utsouthwestern.edu)" > Sent: Wednesday, January 9, 2013 10:55 AM > Subject: [Histonet] Microtome blades > > Does anyone know who I can purchase Accu-edge blades from, other than Cardinal or American Master Tech? > > Laurie Colbert, HT (ASCP) > Histology Supervisor > PATH MD > 8158 Beverly Blvd. > Los Angeles, CA 90048 > (323) 648-3214 direct > (424) 245-7284 main lab > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 110, Issue 9 > **************************************** From madeleinehuey <@t> gmail.com Wed Jan 9 12:07:05 2013 From: madeleinehuey <@t> gmail.com (Madeleine Huey) Date: Wed Jan 9 12:07:09 2013 Subject: [Histonet] Re: Histonet Digest, Vol 110, Issue 9 In-Reply-To: References: <50eda5c0.448db60a.1aa5.2a45SMTPIN_ADDED_MISSING@mx.google.com> Message-ID: Hello Gale, I normally ask the candidates questions relating to embedding/cutting/special stains. For example Special Stains; - how do they perform a test for collagen and smooth muscle (answer - Special Stain "Trichrome"). If they answer correctly with Trichrome, then ask them WHY Trichrome stain (answer - increases in collagenous tissues in diseases such as cirrhosis of the Liver) They just finished the ASCP exam, that mean they should know the theory, not just memorized them & give back to the text book. For Embedding; - how do they perform orientation (ie. G.I., skin, & etc). For routine Cutting; - how thick should one cut routine tissues (H&E) - ___micron thickness Amyloid special stain (should be thick) - ___micron thickness brain tissues (thick & water-bath temperature is crucial as well) - ___micron PAS/D special stain on Kidney tissues After personal interview, I test potential candidate with embedding & cutting on site. Some candidates are great taking theory exam, but not very good with hands or detail. This is my way of testing, does not mean it's the correct way. madeleine_h@elcaminohospital.org Hello, I just found out today I will be doing 2nd interviews for 3 candidates for a part time Histology position at our hospital on Friday of this week. These candidates are not histotechs but are willing to do online training and take ASCP board exam within 24 months. I would appreciate some help with what questions to ask. I did not attend the 1st interviews but these were done by our lab manager who does not know a lot about what we do I histology............. Thank you! Gale Limron CT,HT (ASCP) Histology Supervisor Union Hospital 659 Boulevard Dover, Ohio 44622 330-343-3311 ext 2562 From joelleweaver <@t> hotmail.com Wed Jan 9 12:31:04 2013 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Wed Jan 9 12:31:09 2013 Subject: [Histonet] interview In-Reply-To: <7722595275A4DD4FA225B92CDBF174A101A7F64680DE@EXC-MBX3.cfs.le.ac.uk> References: , , <7722595275A4DD4FA225B92CDBF174A101A7F64680DE@EXC-MBX3.cfs.le.ac.uk> Message-ID: Yes, please interview and hire people with experience and/or training! The situation in histology will never get better otherwise. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: ree3@leicester.ac.uk > To: wdesalvo.cac@outlook.com; galel@unionhospital.org; histonet@lists.utsouthwestern.edu > Date: Wed, 9 Jan 2013 08:50:43 +0000 > Subject: RE: [Histonet] interview > CC: > > Well if you only pay peanuts you only get monkeys...................... > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO > Sent: 09 January 2013 00:05 > To: Gale Limron; histonet > Subject: RE: [Histonet] interview > > It is difficult to interview individuals that do not have any experience in the detailed and manual technology of the Histology lab, but you can find the right individuals to become exceptional Histotechnologists. Although it can be very time consuming to train individuals, the right individual worthy of all the effort must have the right attitude! If the individuals have the necessary science background to understand the complex processes used in Histotechnology and have the right attitude, then lack of aptitude can be overcome. I have been involved, for several years, in training individuals with no Histology experienced and have been always rewarded by the performance of the individuals that wanted to learn and become more than a embedding or microtomy techie. Histotechnology is so much more than slapping tissue into a mold or hacking paraffin sections off a block. Everything done in the Histology lab is directly tied to a patient outcome and quality and precision area must. Make sure the individuals understand how difficult it will be to gain the knowledge and experience necessary to pass the ASCP exam, while only working part-time for 24 months. Find out what type of commitment they are willing to make to better Histotechnology, the patient outcome and themselves before you and your team invest any effort. Above all, the right attitude is the big first step in becoming a competent Histotechnologist that is dedicated and compassionate about improving the patient experience. > > William DeSalvo, B.S., HTL(ASCP) > > Production Manager-Anatomic Pathology > Chair, NSH Quality Management Committee > > Owner/Consultant, Collaborative Advantage Consulting > > > From: GaleL@unionhospital.org > > To: histonet@lists.utsouthwestern.edu > > Date: Tue, 8 Jan 2013 13:02:04 -0500 > > Subject: [Histonet] interview > > > > Hello, > > I just found out today I will be doing 2nd interviews for 3 candidates for a part time Histology position at our hospital on Friday of this week. These candidates are not histotechs but are willing to do online training and take ASCP board exam within 24 months. I would appreciate some help with what questions to ask. I did not attend the 1st interviews but these were done by our lab manager who does not know a lot about what we do I histology............. > > Thank you! > > > > Gale Limron CT,HT (ASCP) > > Histology Supervisor > > Union Hospital > > 659 Boulevard > > Dover, Ohio 44622 > > 330-343-3311 ext 2562 > > > > > > > > This e-mail is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential or otherwise protected from disclosure. Dissemination, distribution or copying of this e-mail or the information herein by anyone other than the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, is prohibited. If you received this message in error, please delete without copying and kindly e-mail a reply to inform us of the mistake in delivery._______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Heidi.Hawthorne <@t> onassignment.com Wed Jan 9 12:31:35 2013 From: Heidi.Hawthorne <@t> onassignment.com (Heidi Hawthorne) Date: Wed Jan 9 12:31:43 2013 Subject: [Histonet] Position Available- San Francisco Bay Area Message-ID: <519812AD3D64B24C9311DB9774CF73E23F31F923@oasslcexm03.oaifield.onasgn.com> On Assignment Healthcare is currently looking for a Histotech to work in an acute care facility in the San Francisco Bay Area. This position is Monday-Friday 5:30am-1:30pm (part time availability may be considered). The position will require paraffin embedding, grossing, cutting, and fixing specimens as well as related work. The ideal Candidate will possess: * A minimum of an Associate degree in a related field * 1-4 years of experience in a hospital laboratory For immediate consideration, please apply with a copy of your resume today! Heidi Hawthorne t: (510) 663-8622 Heidi.Hawthorne@onassignment.com www.onassignment.com From mucram11 <@t> comcast.net Wed Jan 9 12:37:36 2013 From: mucram11 <@t> comcast.net (Pam Marcum) Date: Wed Jan 9 12:37:44 2013 Subject: [Histonet] interview In-Reply-To: Message-ID: <117995865.193203.1357756656174.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> I totally understand hiring only experienced people however; I have a question.? What do you do when you have no one available and the institute you work for will not help with moving expenses or sign on bonuses?? Believe me I know about training OJT today when you are shortstaffed and can't meet salary demands.? Pam Marcum ----- Original Message ----- From: "joelle weaver" To: ree3@leicester.ac.uk, "wdesalvo cac" , galel@unionhospital.org, histonet@lists.utsouthwestern.edu Sent: Wednesday, January 9, 2013 12:31:04 PM Subject: RE: [Histonet] interview Yes, please ?interview and hire people with experience and/or training! ?The situation in histology will never get better otherwise. Joelle Weaver MAOM, HTL (ASCP) QIHC ?> From: ree3@leicester.ac.uk > To: wdesalvo.cac@outlook.com; galel@unionhospital.org; histonet@lists.utsouthwestern.edu > Date: Wed, 9 Jan 2013 08:50:43 +0000 > Subject: RE: [Histonet] interview > CC: > > Well ?if ?you ?only ?pay ?peanuts you ? only ?get ?monkeys...................... > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO > Sent: 09 January 2013 00:05 > To: Gale Limron; histonet > Subject: RE: [Histonet] interview > > It is difficult to interview individuals that do not have any experience in the detailed and manual technology of the Histology lab, but you can find the right individuals to become exceptional Histotechnologists. Although it can be very time consuming to train individuals, the right individual worthy of all the effort must have the right attitude! If the individuals have the necessary science background to understand the complex processes used in Histotechnology and have the right attitude, then lack of aptitude can be overcome. I have been involved, for several years, in training individuals with no Histology experienced and have been always rewarded by the performance of the individuals that wanted to learn and become more than a embedding or microtomy techie. Histotechnology is so much more than slapping tissue into a mold or hacking paraffin sections off a block. Everything done in the Histology lab is directly tied to a patient outcome and quality and precision area must. ?Make sure the individuals understand how difficult it will be to gain the knowledge and experience necessary to pass the ASCP exam, while only working part-time for 24 months. Find out what type of commitment they are willing to make to better Histotechnology, the patient outcome and themselves before you and your team invest any effort. Above all, the right attitude is the big first step in becoming a competent Histotechnologist that is dedicated and compassionate about improving the patient experience. > > William DeSalvo, B.S., HTL(ASCP) > > Production Manager-Anatomic Pathology > Chair, NSH Quality Management Committee > > Owner/Consultant, Collaborative Advantage Consulting > > ?> From: GaleL@unionhospital.org > > To: histonet@lists.utsouthwestern.edu > > Date: Tue, 8 Jan 2013 13:02:04 -0500 > > Subject: [Histonet] interview > > > > Hello, > > I just found out today I will be doing 2nd interviews for 3 candidates for a part time Histology position at our hospital on Friday of this week. These candidates are not histotechs but are willing to do online training and take ASCP board exam within 24 months. I would appreciate some help with what questions to ask. I did not attend the 1st interviews but these were done by our lab manager who does not know a lot about what we do I histology............. > > Thank you! > > > > Gale Limron CT,HT (ASCP) > > Histology Supervisor > > Union Hospital > > 659 Boulevard > > Dover, Ohio 44622 > > 330-343-3311 ext 2562 > > > > > > > > This e-mail is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential or otherwise protected from disclosure. Dissemination, distribution or copying of this e-mail or the information herein by anyone other than the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, is prohibited. If you received this message in error, please delete without copying and kindly e-mail a reply to inform us of the mistake in delivery._______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ????????????????? ???????? ? ???????????????? ?_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ????????????????? ???????? ? ???????????????? ?_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Wed Jan 9 12:38:58 2013 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Wed Jan 9 12:39:05 2013 Subject: [Histonet] Call for Speakers Message-ID: The California Society for Histotechnology is holding their annual symposium May 3-5 at the beautiful Hilton San Francisco Airport Bay Front Hotel. We are looking for speakers. If you are interested in speaking please contact me. Thank you, Jennifer MacDonald CSH Secretary jmacdonaldcsh@gmail.com 760.963.0123 From estellamireles <@t> gmail.com Wed Jan 9 13:26:15 2013 From: estellamireles <@t> gmail.com (Stella Mireles) Date: Wed Jan 9 13:26:18 2013 Subject: [Histonet] Reagent Alc or Denatured Alc ? Message-ID: What is the difference between the two ? Thinking about switching to denatured becasue of cost costing. Which do you prefer? Stella From lpjones <@t> srhs-pa.org Wed Jan 9 13:37:41 2013 From: lpjones <@t> srhs-pa.org (Jones, Laura) Date: Wed Jan 9 13:37:37 2013 Subject: [Histonet] Xylene Free Processing? Message-ID: <4AE8039AEA096143B965CBC6D092166802351B39DB@EXCH2007.srhs-pa.org> I would appreciate hearing all of your expert opinions on processing tissue without the use of xylene or any type of xylene substitute. Our processor was down recently, and a friendly local lab processed our tissue this way for us. Of course, the Pathologists loved it. The process was formalin, followed by graduated percentages of only isopropyl alcohol, and then paraffin. What are your feelings concerning immunos? Overall cost? Any other thoughts? Thanks in advance! ________________________________ Sharon Regional Health System is the area's largest hospital and provider of health care services. Visit us online at http://www.sharonregional.com for a complete listing of our services, primary care physicians and specialists, and satellite locations. Confidentiality Note: This message is intended for use only by the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. Thank you. From wbenton <@t> cua.md Wed Jan 9 13:38:51 2013 From: wbenton <@t> cua.md (Walter Benton) Date: Wed Jan 9 13:39:22 2013 Subject: [Histonet] Reagent Alc or Denatured Alc ? In-Reply-To: References: Message-ID: <0B8979A204680A42B93A52B486088CD92CCF62B8E6@CUAEXH1.GCU-MD.local> They are one in the same basically, meaning not fit for human consumption, by means of adding methanol or other impurity. Pure ethanol would require an alcohol license and inventory tracking since it is fit for human consumption. Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 126 (All Deliveries to Suite 127) Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) wbenton@cua.md ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stella Mireles [estellamireles@gmail.com] Sent: Wednesday, January 09, 2013 2:26 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Reagent Alc or Denatured Alc ? What is the difference between the two ? Thinking about switching to denatured becasue of cost costing. Which do you prefer? Stella _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From allyse124 <@t> gmail.com Wed Jan 9 13:52:10 2013 From: allyse124 <@t> gmail.com (Allyse Mazzarelli) Date: Wed Jan 9 13:52:13 2013 Subject: [Histonet] Immunohistochemistry Message-ID: To everyone in histoland that is familiar with IHC, I am a new histologist, unfamiliar with many techniques in IHC. Here in my lab, we section devices that contain cells, membrane and scaffolding. Unfortunately, due to the scaffolding, the devices do not hold up well when embedded in paraffin. Likewise, the other plastics I am able to cut nice sections with do not take well to immuno staining. Does anyone have any suggestions for a specific type of plastic resin that works well with IHC? (I could try something other than plastic too). For the time being, I use Dorn & Hart acrylosin soft... but the results are medicore. We will be doing a lot more immunohistochemistry in the near future, and I'm looking to experiment with different resins to find one that works well. Any help is appreciated! Thanks for your time! Sincerely, Allyse Mazzarelli Histologist, Neurotech USA Inc. From rgeske_2000 <@t> yahoo.com Wed Jan 9 13:53:20 2013 From: rgeske_2000 <@t> yahoo.com (Rob Geske) Date: Wed Jan 9 13:53:25 2013 Subject: [Histonet] interview In-Reply-To: <117995865.193203.1357756656174.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Message-ID: <1357761200.47976.YahooMailClassic@web160501.mail.bf1.yahoo.com> Hi Pam, if there is no one qualified locally i would suggest you look to a temp agency that does have qualified individuals that can provide the skill sets you require.? if someone needs to be brought in from outside your area, the agency will certainly charge you per diem in addition to salary plus their agency fee.? once you extrapolate all of those costs out over 6 months to a year i expect you will find that you will be able to make a convincing case for paying either a sign on bonus or moving expenses.? the other option is to scale the volume down to a point where it is manageable with the resources you have and are able to maintain the same quality.? there have been a number of temp agencies that have posted here -- i expect they could be a valuable resource to you. regards, rob --- On Wed, 1/9/13, Pam Marcum wrote: From: Pam Marcum Subject: Re: [Histonet] interview To: "joelle weaver" Cc: histonet@lists.utsouthwestern.edu, galel@unionhospital.org Date: Wednesday, January 9, 2013, 12:37 PM I totally understand hiring only experienced people however; I have a question.? What do you do when you have no one available and the institute you work for will not help with moving expenses or sign on bonuses?? Believe me I know about training OJT today when you are shortstaffed and can't meet salary demands.? Pam Marcum ----- Original Message ----- From: "joelle weaver" To: ree3@leicester.ac.uk, "wdesalvo cac" , galel@unionhospital.org, histonet@lists.utsouthwestern.edu Sent: Wednesday, January 9, 2013 12:31:04 PM Subject: RE: [Histonet] interview Yes, please ?interview and hire people with experience and/or training! ?The situation in histology will never get better otherwise. Joelle Weaver MAOM, HTL (ASCP) QIHC ?> From: ree3@leicester.ac.uk > To: wdesalvo.cac@outlook.com; galel@unionhospital.org; histonet@lists.utsouthwestern.edu > Date: Wed, 9 Jan 2013 08:50:43 +0000 > Subject: RE: [Histonet] interview > CC: > > Well ?if ?you ?only ?pay ?peanuts you ? only ?get ?monkeys...................... > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO > Sent: 09 January 2013 00:05 > To: Gale Limron; histonet > Subject: RE: [Histonet] interview > > It is difficult to interview individuals that do not have any experience in the detailed and manual technology of the Histology lab, but you can find the right individuals to become exceptional Histotechnologists. Although it can be very time consuming to train individuals, the right individual worthy of all the effort must have the right attitude! If the individuals have the necessary science background to understand the complex processes used in Histotechnology and have the right attitude, then lack of aptitude can be overcome. I have been involved, for several years, in training individuals with no Histology experienced and have been always rewarded by the performance of the individuals that wanted to learn and become more than a embedding or microtomy techie. Histotechnology is so much more than slapping tissue into a mold or hacking paraffin sections off a block. Everything done in the Histology lab is directly tied to a patient outcome and quality and precision area must. ?Make sure the individuals understand how difficult it will be to gain the knowledge and experience necessary to pass the ASCP exam, while only working part-time for 24 months. Find out what type of commitment they are willing to make to better Histotechnology, the patient outcome and themselves before you and your team invest any effort. Above all, the right attitude is the big first step in becoming a competent Histotechnologist that is dedicated and compassionate about improving the patient experience. > > William DeSalvo, B.S., HTL(ASCP) > > Production Manager-Anatomic Pathology > Chair, NSH Quality Management Committee > > Owner/Consultant, Collaborative Advantage Consulting > > ?> From: GaleL@unionhospital.org > > To: histonet@lists.utsouthwestern.edu > > Date: Tue, 8 Jan 2013 13:02:04 -0500 > > Subject: [Histonet] interview > > > > Hello, > > I just found out today I will be doing 2nd interviews for 3 candidates for a part time Histology position at our hospital on Friday of this week. These candidates are not histotechs but are willing to do online training and take ASCP board exam within 24 months. I would appreciate some help with what questions to ask. I did not attend the 1st interviews but these were done by our lab manager who does not know a lot about what we do I histology............. > > Thank you! > > > > Gale Limron CT,HT (ASCP) > > Histology Supervisor > > Union Hospital > > 659 Boulevard > > Dover, Ohio 44622 > > 330-343-3311 ext 2562 > > > > > > > > This e-mail is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential or otherwise protected from disclosure. Dissemination, distribution or copying of this e-mail or the information herein by anyone other than the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, is prohibited. If you received this message in error, please delete without copying and kindly e-mail a reply to inform us of the mistake in delivery._______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ????????????????? ???????? ? ???????????????? ?_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ????????????????? ???????? ? ???????????????? ?_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hlukey <@t> msn.com Wed Jan 9 14:00:07 2013 From: hlukey <@t> msn.com (Hugh Luk) Date: Wed Jan 9 14:00:12 2013 Subject: [Histonet] RE: Microtome blades In-Reply-To: References: Message-ID: Laurie, Accuedge can also be found through: VWR (https://us.vwr.com/store/catalog/product.jsp?catalog_number=25608-964) or Electron Microscopy Services http://www.emsdiasum.com/microscopy/products/histology/sectioning.aspx I believe we're in a similar situation, as our institution has "Favored" vendors (volume discounts), so we had to look this up once or twice. Hope this helps, Hugh UH cancer center > Date: Wed, 9 Jan 2013 09:13:55 -0700 > From: Elizabeth Chlipala > Subject: [Histonet] RE: Microtome blades > To: 'Laurie Colbert' , "Histonet Post > (histonet@lists.utsouthwestern.edu)" > > Message-ID: > <14E2C6176416974295479C64A11CB9AE016411ABF729@SBS2K8.premierlab.local> > Content-Type: text/plain; charset="us-ascii" > > Mercedes Medical or directly from Sakura > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > Manager > Premier Laboratory, LLC > PO Box 18592 > Boulder, CO 80308-1592 > (303) 682-3949 office > (303) 682-9060 fax > (303) 881-0763 cell > www.premierlab.com > > Ship to address: > > 1567 Skyway Drive, Unit E > Longmont, CO 80504 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert > Sent: Wednesday, January 09, 2013 8:56 AM > To: Histonet Post (histonet@lists.utsouthwestern.edu) > Subject: [Histonet] Microtome blades > > Does anyone know who I can purchase Accu-edge blades from, other than Cardinal or American Master Tech? > > Laurie Colbert, HT (ASCP) > Histology Supervisor > PATH MD > 8158 Beverly Blvd. > Los Angeles, CA 90048 > (323) 648-3214 direct > (424) 245-7284 main lab > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From billodonnell <@t> catholichealth.net Wed Jan 9 14:15:37 2013 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Wed Jan 9 14:16:06 2013 Subject: [Histonet] Xylene Free Processing? In-Reply-To: <4AE8039AEA096143B965CBC6D092166802351B39DB@EXCH2007.srhs-pa.org> References: <4AE8039AEA096143B965CBC6D092166802351B39DB@EXCH2007.srhs-pa.org> Message-ID: <4940DF6D1C5FDF48931B6966AAEF9395940C1C@chimsx08.CHI.catholichealth.net> The Xpress microwave tx processors are xylene free - perhaps they have one. I am pretty certain there still needs to be something that is misable with alcohol and paraffin, such as mineral oil. I believe this can be added to one or two of the paraffin steps, but I am just venturing a guess. Most processors would still require a xylene (or substitute) cleaning cycle to remove paraffin. I will be following this thread with interest - Bill William (Bill) O'Donnell, HT (ASCP) QIHC Senior Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 SERENITY is not freedom from the storm, but peace amid the storm. Cultivate it in PRAYER! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jones, Laura Sent: Wednesday, January 09, 2013 1:38 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Xylene Free Processing? I would appreciate hearing all of your expert opinions on processing tissue without the use of xylene or any type of xylene substitute. Our processor was down recently, and a friendly local lab processed our tissue this way for us. Of course, the Pathologists loved it. The process was formalin, followed by graduated percentages of only isopropyl alcohol, and then paraffin. What are your feelings concerning immunos? Overall cost? Any other thoughts? Thanks in advance! ________________________________ Sharon Regional Health System is the area's largest hospital and provider of health care services. Visit us online at http://www.sharonregional.com for a complete listing of our services, primary care physicians and specialists, and satellite locations. Confidentiality Note: This message is intended for use only by the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. From rjbuesa <@t> yahoo.com Wed Jan 9 14:45:44 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jan 9 14:45:49 2013 Subject: [Histonet] Xylene Free Processing? In-Reply-To: <4AE8039AEA096143B965CBC6D092166802351B39DB@EXCH2007.srhs-pa.org> References: <4AE8039AEA096143B965CBC6D092166802351B39DB@EXCH2007.srhs-pa.org> Message-ID: <1357764344.9207.YahooMailNeo@web163104.mail.bf1.yahoo.com> Please go to http://www.histosearch.com/rene.html and you will find my articles on the sibject. Ren? J. From: "Jones, Laura" To: "Histonet@lists.utsouthwestern.edu" Sent: Wednesday, January 9, 2013 2:37 PM Subject: [Histonet] Xylene Free Processing? I would appreciate hearing all of your expert opinions on processing tissue without the use of xylene or any type of xylene substitute.? Our processor was down recently, and a friendly local lab processed our tissue this way for us.? Of course, the Pathologists loved it.? The process was formalin, followed by graduated percentages of only isopropyl alcohol, and then paraffin. What are your feelings concerning immunos?? Overall cost?? Any other thoughts? Thanks in advance! ________________________________ Sharon Regional Health System is the area's largest hospital and provider of health care services. Visit us online at http://www.sharonregional.com for a complete listing of our services, primary care physicians and specialists, and satellite locations. Confidentiality Note: This message is intended for use only by the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sdysart <@t> mirnarx.com Wed Jan 9 14:48:06 2013 From: sdysart <@t> mirnarx.com (Sarah Dysart) Date: Wed Jan 9 14:48:20 2013 Subject: [Histonet] Xylene Free Processing? In-Reply-To: <1357764344.9207.YahooMailNeo@web163104.mail.bf1.yahoo.com> References: <4AE8039AEA096143B965CBC6D092166802351B39DB@EXCH2007.srhs-pa.org> <1357764344.9207.YahooMailNeo@web163104.mail.bf1.yahoo.com> Message-ID: <8A70A9B2ECDD084DACFE6C59FCF86D5047B4CEC8@BL2PRD0710MB363.namprd07.prod.outlook.com> FYI, I've tried ClearRite, and while I guess it technically works...it's a giant pain to work with because you have to extend out your times. I also found that it screws with Eosin because if it gets moisture in it (which you can't see); the water bleaches out the Eosin. Just my two cents worth =) Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, January 09, 2013 2:46 PM To: Jones, Laura; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Xylene Free Processing? Please go to http://www.histosearch.com/rene.html and you will find my articles on the sibject. Ren? J. From: "Jones, Laura" To: "Histonet@lists.utsouthwestern.edu" Sent: Wednesday, January 9, 2013 2:37 PM Subject: [Histonet] Xylene Free Processing? I would appreciate hearing all of your expert opinions on processing tissue without the use of xylene or any type of xylene substitute.? Our processor was down recently, and a friendly local lab processed our tissue this way for us.? Of course, the Pathologists loved it.? The process was formalin, followed by graduated percentages of only isopropyl alcohol, and then paraffin. What are your feelings concerning immunos?? Overall cost?? Any other thoughts? Thanks in advance! ________________________________ Sharon Regional Health System is the area's largest hospital and provider of health care services. Visit us online at http://www.sharonregional.com for a complete listing of our services, primary care physicians and specialists, and satellite locations. Confidentiality Note: This message is intended for use only by the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Jan 9 14:48:38 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jan 9 14:48:43 2013 Subject: [Histonet] Xylene Free Processing? In-Reply-To: <4940DF6D1C5FDF48931B6966AAEF9395940C1C@chimsx08.CHI.catholichealth.net> References: <4AE8039AEA096143B965CBC6D092166802351B39DB@EXCH2007.srhs-pa.org> <4940DF6D1C5FDF48931B6966AAEF9395940C1C@chimsx08.CHI.catholichealth.net> Message-ID: <1357764518.95114.YahooMailNeo@web163106.mail.bf1.yahoo.com> A mixture of isopropyl alcohol and mineral oil is the best solution to complete a "gentle and graded" tissue processing. To clean the tissue processor you can use a 5% solution of dishwasher soap in isopropyl alcohol. You can find my article on the subject in http://www.histosearch.com/rene.html Ren? J.? From: "O'Donnell, Bill" To: "Jones, Laura" ; Histonet@lists.utsouthwestern.edu Sent: Wednesday, January 9, 2013 3:15 PM Subject: RE: [Histonet] Xylene Free Processing? The Xpress microwave tx processors are xylene free - perhaps they have one. I am pretty certain there still needs to be something that is misable with alcohol and paraffin, such as mineral oil. I believe this can be added to one or two of the paraffin steps, but I am just venturing a guess. Most processors would still require a xylene (or substitute) cleaning cycle to remove paraffin. I will be following this thread with interest - Bill William (Bill) O'Donnell, HT (ASCP) QIHC Senior Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 SERENITY is not freedom from the storm, but peace amid the storm. Cultivate it in PRAYER! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jones, Laura Sent: Wednesday, January 09, 2013 1:38 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Xylene Free Processing? I would appreciate hearing all of your expert opinions on processing tissue without the use of xylene or any type of xylene substitute.? Our processor was down recently, and a friendly local lab processed our tissue this way for us.? Of course, the Pathologists loved it.? The process was formalin, followed by graduated percentages of only isopropyl alcohol, and then paraffin. What are your feelings concerning immunos?? Overall cost?? Any other thoughts? Thanks in advance! ________________________________ Sharon Regional Health System is the area's largest hospital and provider of health care services. Visit us online at http://www.sharonregional.com for a complete listing of our services, primary care physicians and specialists, and satellite locations. Confidentiality Note: This message is intended for use only by the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TNMayer <@t> mdanderson.org Wed Jan 9 15:21:59 2013 From: TNMayer <@t> mdanderson.org (Mayer,Toysha N) Date: Wed Jan 9 15:23:22 2013 Subject: [Histonet] RE: Histonet Digest, Vol 110, Issue 9 In-Reply-To: <403c6b07-a16b-4f5d-b1fd-42fa9b9a3640@DCPWPRTR01.mdanderson.edu> References: <403c6b07-a16b-4f5d-b1fd-42fa9b9a3640@DCPWPRTR01.mdanderson.edu> Message-ID: <47E9B2C01DDDD94881EACD2DC44EBC88021497@D1PWPEXMBX05.mdanderson.edu> Amy, Try contacting the local hospitals for some blocks. For trichrome and mucin, you can use small intestine. Those are usually readily available from a local facility. Iron uses liver or spleen and those can be obtained the same way. By networking with other facilities you can begin a barter system for obtaining controls. I can send you an iron control block next week, is the address provided correct? As for purchasing controls, only get those that you cannot get normal tissue for, like microorganisms. Good luck, Toysha N. Mayer, MBA, HT (ASCP) Instructor, Education Coordinator Program in Histotechnology School of Health Professions MD Anderson Cancer Center (713) 563-3481 tnmayer@mdanderson.org ------------------------------ Message: 9 Date: Wed, 9 Jan 2013 00:07:09 +0000 From: "Johnston, Amy" Subject: [Histonet] Control Tissue To: "histonet@lists.utsouthwestern.edu" Message-ID: <8F6C0286892B2544B10E5F31F1C7075D27B815CF@IPSPRDMBOX1.oregonmed.net> Content-Type: text/plain; charset="us-ascii" Is there any way to obtain control blocks? We are in the process of starting up a new histology lab and control slides are a lot more expensive than I thought:( . I have purchased several boxes already, I was hoping I could find the others as they should be easy to find. (I tried NSH, they no longer have a bank of blocks.) I still need controls for trichrome, iron and mucin, any suggestions? Amy Johnston HT(ASCP) Histology Technician Oregon Medical Group 4140 Quest Drive Eugene, OR 97402 541-463-2163 From joelleweaver <@t> hotmail.com Wed Jan 9 15:29:30 2013 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Wed Jan 9 15:29:37 2013 Subject: [Histonet] interview In-Reply-To: <117995865.193203.1357756656174.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> References: , <117995865.193203.1357756656174.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Message-ID: Yes, I understand that it can be a difficult situation. I just feel that so long as it continues as it has, we are likely to get the same result. If you have no applicants who are trained or experienced, and you cannot "lure" someone with experience and training by incentives, then I feel you have to really be prepared to commit to the training process and way past the usual "orientation" period. If you only have new entrants then you have to try to select for people who have the basic science and chemistry background to build on, otherwise they will never "get" theory or the "why" because they have not been given the tools for that. They will have real difficulties troubleshooting and problem-solving. All of healthcare needs more problem solvers and innovators, and if we have to build them, so be it I say. A good attitude doesn't hurt either, and caring about what you do, both are very hard to train, but can be strengthened in the right individuals, by positive reinforcement and example. I feel there are no shortcuts here, otherwise you will end up with people with very limited scope and you will fall into what I call the "adding bodies" not capabilities syndrome. Some will know what I mean here. Also you will be doing them a injustice if they decide to go out into the field and into market areas where credential and competency requirements are more strict. They have not be able to do OJT certification for quite some time, (without the college credits), and so I think that you owe them that information about the state of the industry, so they can decide if they want to invest of themselves for the education and training needed.Otherwise their opportunties may become increasingly limited. I also understand the commitment and time involved in training people from "ground zero" from my service as a faculty/clinical instructor and program director in an HT program, and lots of personal time spent training people on the bench in between my "regular" assignments. So I am not without empathy to the energy required, the lack of support that can happen, and other factors. But I also know that all education has ancillary benefits, such as tolerance for others and communication skills that are very useful in any job, and which I choose to believe help make the work of education worthwhile. To help enrich our field, I feel strongly that we need to support the movement towards greater education and professional identity. Hiring supervisors and managers should try to stay as firm as they can on this- that is my opinion only. Overall, I just know from seeing this play out many times, that it will not change if we do not change the way we approach it. Believe me, if I had the power to make this situation better and magically come up with the resources, support and manpower needed to give people the training and opportunity they might deserve, I certainly would do it. Joelle Weaver MAOM, HTL (ASCP) QIHC Date: Wed, 9 Jan 2013 18:37:36 +0000 From: mucram11@comcast.net To: joelleweaver@hotmail.com CC: ree3@leicester.ac.uk; wdesalvo.cac@outlook.com; galel@unionhospital.org; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] interview I totally understand hiring only experienced people however; I have a question. What do you do when you have no one available and the institute you work for will not help with moving expenses or sign on bonuses? Believe me I know about training OJT today when you are shortstaffed and can't meet salary demands. Pam Marcum From: "joelle weaver" To: ree3@leicester.ac.uk, "wdesalvo cac" , galel@unionhospital.org, histonet@lists.utsouthwestern.edu Sent: Wednesday, January 9, 2013 12:31:04 PM Subject: RE: [Histonet] interview Yes, please interview and hire people with experience and/or training! The situation in histology will never get better otherwise. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: ree3@leicester.ac.uk > To: wdesalvo.cac@outlook.com; galel@unionhospital.org; histonet@lists.utsouthwestern.edu > Date: Wed, 9 Jan 2013 08:50:43 +0000 > Subject: RE: [Histonet] interview > CC: > > Well if you only pay peanuts you only get monkeys...................... > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO > Sent: 09 January 2013 00:05 > To: Gale Limron; histonet > Subject: RE: [Histonet] interview > > It is difficult to interview individuals that do not have any experience in the detailed and manual technology of the Histology lab, but you can find the right individuals to become exceptional Histotechnologists. Although it can be very time consuming to train individuals, the right individual worthy of all the effort must have the right attitude! If the individuals have the necessary science background to understand the complex processes used in Histotechnology and have the right attitude, then lack of aptitude can be overcome. I have been involved, for several years, in training individuals with no Histology experienced and have been always rewarded by the performance of the individuals that wanted to learn and become more than a embedding or microtomy techie. Histotechnology is so much more than slapping tissue into a mold or hacking paraffin sections off a block. Everything done in the Histology lab is directly tied to a patient outcome and quality and precision area must. Make sure the individuals understand how difficult it will be to gain the knowledge and experience necessary to pass the ASCP exam, while only working part-time for 24 months. Find out what type of commitment they are willing to make to better Histotechnology, the patient outcome and themselves before you and your team invest any effort. Above all, the right attitude is the big first step in becoming a competent Histotechnologist that is dedicated and compassionate about improving the patient experience. > > William DeSalvo, B.S., HTL(ASCP) > > Production Manager-Anatomic Pathology > Chair, NSH Quality Management Committee > > Owner/Consultant, Collaborative Advantage Consulting > > > From: GaleL@unionhospital.org > > To: histonet@lists.utsouthwestern.edu > > Date: Tue, 8 Jan 2013 13:02:04 -0500 > > Subject: [Histonet] interview > > > > Hello, > > I just found out today I will be doing 2nd interviews for 3 candidates for a part time Histology position at our hospital on Friday of this week. These candidates are not histotechs but are willing to do online training and take ASCP board exam within 24 months. I would appreciate some help with what questions to ask. I did not attend the 1st interviews but these were done by our lab manager who does not know a lot about what we do I histology............. > > Thank you! > > > > Gale Limron CT,HT (ASCP) > > Histology Supervisor > > Union Hospital > > 659 Boulevard > > Dover, Ohio 44622 > > 330-343-3311 ext 2562 > > > > > > > > This e-mail is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential or otherwise protected from disclosure. Dissemination, distribution or copying of this e-mail or the information herein by anyone other than the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, is prohibited. If you received this message in error, please delete without copying and kindly e-mail a reply to inform us of the mistake in delivery._______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> gmail.com Wed Jan 9 16:56:01 2013 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Wed Jan 9 16:56:06 2013 Subject: [Histonet] Re: Reagent alcohol or denatured alcohol Message-ID: Stella Mireles asks: >>Reagent Alcohol or Denatured Alcohol? What is the difference between the two? Thinking about switching to denatured because of cost costing. Which do you prefer?<< Reagent alcohol is a commonly used term for ethanol (ethyl alcohol) to which 5% methanol and 5% isopropanol have been added to keep people from drinking it. This mixture is widely used in histology. Denatured alcohol is ethanol that has been made undrinkable by adding various substances that are prescribed by the authorities using standard formulas. (Reagent alcohol is "S3DA modified".) Most of these mixtures are unsuitable for histologic use. Ethanol denatured with methyl isobutyl ketone (MIBK) is occasionally offered and can be used histologically, but it smells so bad that most people won't use it. Ethanol denatured with acetone is not suitable, because the acetone dissolves the eosin from your sections. Bob Richmond Samurai Pathologist Maryville TN From SFinley <@t> providencehealth.bc.ca Wed Jan 9 17:12:06 2013 From: SFinley <@t> providencehealth.bc.ca (Finley, Sue [PH]) Date: Wed Jan 9 17:12:12 2013 Subject: [Histonet] Xylene Free Processing? In-Reply-To: <4940DF6D1C5FDF48931B6966AAEF9395940C1C@chimsx08.CHI.catholichealth.net> References: <4AE8039AEA096143B965CBC6D092166802351B39DB@EXCH2007.srhs-pa.org> <4940DF6D1C5FDF48931B6966AAEF9395940C1C@chimsx08.CHI.catholichealth.net> Message-ID: <7D627F9CD9AE514087E447A9716D4D350141EF5FE4@vchexmbp14.vch.ca> We use xylene free processing + microwave Formalin, 100% ethanol, isopropanol (clearant) and paraffin and have been doing so for 4 years -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: January 9, 2013 12:16 PM To: Jones, Laura; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Xylene Free Processing? The Xpress microwave tx processors are xylene free - perhaps they have one. I am pretty certain there still needs to be something that is misable with alcohol and paraffin, such as mineral oil. I believe this can be added to one or two of the paraffin steps, but I am just venturing a guess. Most processors would still require a xylene (or substitute) cleaning cycle to remove paraffin. I will be following this thread with interest - Bill William (Bill) O'Donnell, HT (ASCP) QIHC Senior Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 SERENITY is not freedom from the storm, but peace amid the storm. Cultivate it in PRAYER! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jones, Laura Sent: Wednesday, January 09, 2013 1:38 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Xylene Free Processing? I would appreciate hearing all of your expert opinions on processing tissue without the use of xylene or any type of xylene substitute. Our processor was down recently, and a friendly local lab processed our tissue this way for us. Of course, the Pathologists loved it. The process was formalin, followed by graduated percentages of only isopropyl alcohol, and then paraffin. What are your feelings concerning immunos? Overall cost? Any other thoughts? Thanks in advance! ________________________________ Sharon Regional Health System is the area's largest hospital and provider of health care services. Visit us online at http://www.sharonregional.com for a complete listing of our services, primary care physicians and specialists, and satellite locations. Confidentiality Note: This message is intended for use only by the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mucram11 <@t> comcast.net Wed Jan 9 19:04:58 2013 From: mucram11 <@t> comcast.net (Pam Marcum) Date: Wed Jan 9 19:05:06 2013 Subject: [Histonet] interview In-Reply-To: Message-ID: <2018966983.203807.1357779898855.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> In the 45 plus years I have been in Histology I seen this many times also.? Unfortunately, we are still the unheard of area of the lab.??T his is not changing through any of the organizations who are happy to take our money and not help us get out who we are and what we do.? I have visited schools for years and talked to young people about Histology, even had them visit the lab.? E e are just not as glamorous as some other areas of the lab they see on TV or hear about at school where the med tech programs are pushed that do not include Histology in the cirrculum.? Sorry to say we have not had a marketing plan for the field, so the pay is low and the hours long; not to mention the responsibility of having the patient's life in our hands as we prepare the specimens. OJT training is only as good as the time can put in it and in a very busy lab it is not enough even if they have the AS they need to take the test. Pam Marcum ----- Original Message ----- From: "joelle weaver" To: mucram11@comcast.net Cc: ree3@leicester.ac.uk, "wdesalvo cac" , galel@unionhospital.org, histonet@lists.utsouthwestern.edu Sent: Wednesday, January 9, 2013 3:29:30 PM Subject: RE: [Histonet] interview Yes, I understand that it can be a difficult situation. I just feel that so long as it continues as it has, we are likely to get the same result. If you have no? applicants who are trained or experienced, and you cannot "lure" someone with experience and training by incentives, ?then I feel you have to really be prepared to commit to the training process and way past the usual "orientation" period. If you only have?new entrants then?you?have to try to select for?people who have the basic science and chemistry background to build on, otherwise they will never "get" theory or the "why" because they have not been given the tools for that. They will have real?difficulties troubleshooting and problem-solving. All?of healthcare needs more problem solvers and innovators, and if we have to build them, so be it I say.?A good attitude doesn't hurt either, and caring about what you do,??both?are very?hard to train, but can be?strengthened in the right individuals, by positive reinforcement and example.?I feel there are no shortcuts here,?otherwise you will end up with people with very limited scope??and you will fall into what I call the "adding bodies" not capabilities syndrome. Some will know what I mean here. Also?you will be doing them a injustice if they decide to go out into the field and?into market areas where credential and competency requirements are more strict. They?have not be able to do OJT certification for quite some time, (without the college credits), and so I think that you owe them that information about the state of the industry, so they can decide if they want to invest of themselves for the education and training needed.Otherwise their opportunties may become increasingly limited.? ?I also understand the commitment and time involved in training people from "ground zero" from my service as a?faculty/clinical instructor and program director?in an HT program, and? lots of personal?time spent training?people on the bench in between my "regular" assignments. So I am not without empathy to the energy required, the lack of support that can happen, and other factors. But?I also know that all?education?has ancillary benefits, such as tolerance for others and communication skills that are very useful?in any job,?and which I choose to believe? help make the work of education worthwhile. To help enrich our field, I feel strongly that?we need to support the movement towards greater education and professional identity. Hiring supervisors and managers should try to stay?as firm as they can on this- that is my opinion only. ?Overall, ?I just know?from seeing this play out many times, that it will not change? if we do not change the way we approach it. Believe me,??if I had the power to make this situation better and magically come up with the resources, support and manpower needed? to give?people the training and opportunity they might deserve, ?I certainly would do it. Joelle Weaver MAOM, HTL (ASCP) QIHC ? Date: Wed, 9 Jan 2013 18:37:36 +0000 From: mucram11@comcast.net To: joelleweaver@hotmail.com CC: ree3@leicester.ac.uk; wdesalvo.cac@outlook.com; galel@unionhospital.org; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] interview I totally understand hiring only experienced people however; I have a question.? What do you do when you have no one available and the institute you work for will not help with moving expenses or sign on bonuses?? ? Believe me I know about training OJT today when you are shortstaffed and can't meet salary demands.? ? Pam Marcum From: "joelle weaver" To: ree3@leicester.ac.uk, "wdesalvo cac" , galel@unionhospital.org, histonet@lists.utsouthwestern.edu Sent: Wednesday, January 9, 2013 12:31:04 PM Subject: RE: [Histonet] interview Yes, please ?interview and hire people with experience and/or training! ?The situation in histology will never get better otherwise. Joelle Weaver MAOM, HTL (ASCP) QIHC ?> From: ree3@leicester.ac.uk > To: wdesalvo.cac@outlook.com; galel@unionhospital.org; histonet@lists.utsouthwestern.edu > Date: Wed, 9 Jan 2013 08:50:43 +0000 > Subject: RE: [Histonet] interview > CC: > > Well ?if ?you ?only ?pay ?peanuts you ? only ?get ?monkeys...................... > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO > Sent: 09 January 2013 00:05 > To: Gale Limron; histonet > Subject: RE: [Histonet] interview > > It is difficult to interview individuals that do not have any experience in the detailed and manual technology of the Histology lab, but you can find the right individuals to become exceptional Histotechnologists. Although it can be very time consuming to train individuals, the right individual worthy of all the effort must have the right attitude! If the individuals have the necessary science background to understand the complex processes used in Histotechnology and have the right attitude, then lack of aptitude can be overcome. I have been involved, for several years, in training individuals with no Histology experienced and have been always rewarded by the performance of the individuals that wanted to learn and become more than a embedding or microtomy techie. Histotechnology is so much more than slapping tissue into a mold or hacking paraffin sections off a block. Everything done in the Histology lab is directly tied to a patient outcome and quality and precision area must. ?Make sure the individuals understand how difficult it will be to gain the knowledge and experience necessary to pass the ASCP exam, while only working part-time for 24 months. Find out what type of commitment they are willing to make to better Histotechnology, the patient outcome and themselves before you and your team invest any effort. Above all, the right attitude is the big first step in becoming a competent Histotechnologist that is dedicated and compassionate about improving the patient experience. > > William DeSalvo, B.S., HTL(ASCP) > > Production Manager-Anatomic Pathology > Chair, NSH Quality Management Committee > > Owner/Consultant, Collaborative Advantage Consulting > > ?> From: GaleL@unionhospital.org > > To: histonet@lists.utsouthwestern.edu > > Date: Tue, 8 Jan 2013 13:02:04 -0500 > > Subject: [Histonet] interview > > > > Hello, > > I just found out today I will be doing 2nd interviews for 3 candidates for a part time Histology position at our hospital on Friday of this week. These candidates are not histotechs but are willing to do online training and take ASCP board exam within 24 months. I would appreciate some help with what questions to ask. I did not attend the 1st interviews but these were done by our lab manager who does not know a lot about what we do I histology............. > > Thank you! > > > > Gale Limron CT,HT (ASCP) > > Histology Supervisor > > Union Hospital > > 659 Boulevard > > Dover, Ohio 44622 > > 330-343-3311 ext 2562 > > > > > > > > This e-mail is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential or otherwise protected from disclosure. Dissemination, distribution or copying of this e-mail or the information herein by anyone other than the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, is prohibited. If you received this message in error, please delete without copying and kindly e-mail a reply to inform us of the mistake in delivery._______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ????????????????? ???????? ? ???????????????? ?_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ????????????????? ???????? ? ???????????????? ?_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From estellamireles <@t> gmail.com Wed Jan 9 20:41:06 2013 From: estellamireles <@t> gmail.com (Stella Mireles) Date: Wed Jan 9 20:41:10 2013 Subject: [Histonet] RE: Microtome blades In-Reply-To: References: Message-ID: I purchase Accu-edge blades from Stat Lab. Thanks to All for the comments on reagent and denatured alcohol. On Wed, Jan 9, 2013 at 2:00 PM, Hugh Luk wrote: > > Laurie, > > Accuedge can also be found through: > > VWR (https://us.vwr.com/store/catalog/product.jsp?catalog_number=25608-964) > or > Electron Microscopy Services > http://www.emsdiasum.com/microscopy/products/histology/sectioning.aspx > > I believe we're in a similar situation, as our institution has "Favored" > vendors (volume discounts), so we had to look this up once or twice. > > Hope this helps, > Hugh > UH cancer center > > > > > Date: Wed, 9 Jan 2013 09:13:55 -0700 > > From: Elizabeth Chlipala > > Subject: [Histonet] RE: Microtome blades > > To: 'Laurie Colbert' , "Histonet Post > > (histonet@lists.utsouthwestern.edu)" > > > > Message-ID: > > > <14E2C6176416974295479C64A11CB9AE016411ABF729@SBS2K8.premierlab.local> > > Content-Type: text/plain; charset="us-ascii" > > > > Mercedes Medical or directly from Sakura > > > > Liz > > > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > > Manager > > Premier Laboratory, LLC > > PO Box 18592 > > Boulder, CO 80308-1592 > > (303) 682-3949 office > > (303) 682-9060 fax > > (303) 881-0763 cell > > www.premierlab.com > > > > Ship to address: > > > > 1567 Skyway Drive, Unit E > > Longmont, CO 80504 > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert > > Sent: Wednesday, January 09, 2013 8:56 AM > > To: Histonet Post (histonet@lists.utsouthwestern.edu) > > Subject: [Histonet] Microtome blades > > > > Does anyone know who I can purchase Accu-edge blades from, other than > Cardinal or American Master Tech? > > > > Laurie Colbert, HT (ASCP) > > Histology Supervisor > > PATH MD > > 8158 Beverly Blvd. > > Los Angeles, CA 90048 > > (323) 648-3214 direct > > (424) 245-7284 main lab > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From histotech <@t> imagesbyhopper.com Thu Jan 10 07:45:37 2013 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Thu Jan 10 07:46:10 2013 Subject: [Histonet] Medical Director credentials on reports Message-ID: <001501cdef38$d2e80340$78b809c0$@imagesbyhopper.com> Does anyone know, and maybe have the regs, whether either the Joint Commission or the CAP *require* that the Medical Director's credentials be included on the genlab and surgical reports? I have heard that it *is* a requirement and now am hearing that it is *not* one. Clarification would be nice! Example: Joe Smith Medical Director OR Joe Smith, M.D. Medical Director Thanks! Michelle ----- No virus found in this message. Checked by AVG - www.avg.com Version: 2013.0.2805 / Virus Database: 2637/6021 - Release Date: 01/09/13 From chapcl <@t> yahoo.com Thu Jan 10 07:58:05 2013 From: chapcl <@t> yahoo.com (Will Chappell) Date: Thu Jan 10 07:58:14 2013 Subject: [Histonet] Medical Director credentials on reports In-Reply-To: <001501cdef38$d2e80340$78b809c0$@imagesbyhopper.com> References: <001501cdef38$d2e80340$78b809c0$@imagesbyhopper.com> Message-ID: <453E2357-6988-4A70-8B48-B21113554C5C@yahoo.com> Just passed CLIA. It is a CLIA requirement. Not sure about CAP. Will Chappell CHOC Children's Sent from my iPhone On Jan 10, 2013, at 5:45 AM, wrote: > Does anyone know, and maybe have the regs, whether either the Joint > Commission or the CAP *require* that the Medical Director's credentials be > included on the genlab and surgical reports? I have heard that it *is* a > requirement and now am hearing that it is *not* one. Clarification would be > nice! > > Example: > > Joe Smith > Medical Director > > OR > > Joe Smith, M.D. > Medical Director > > Thanks! > Michelle > ----- > No virus found in this message. > Checked by AVG - www.avg.com > Version: 2013.0.2805 / Virus Database: 2637/6021 - Release Date: 01/09/13 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LPaveli1 <@t> hurleymc.com Thu Jan 10 08:45:22 2013 From: LPaveli1 <@t> hurleymc.com (Lynette Pavelich) Date: Thu Jan 10 08:46:00 2013 Subject: [Histonet] Medical Director credentials on reports In-Reply-To: <453E2357-6988-4A70-8B48-B21113554C5C@yahoo.com> References: <001501cdef38$d2e80340$78b809c0$@imagesbyhopper.com>, <453E2357-6988-4A70-8B48-B21113554C5C@yahoo.com> Message-ID: <89F4666A496DC949A819ECC40E11C867BF56FABA@EXCHANGEMB1.hmc.hurleymc.com> Searched for the CLIA regulation pertaining to this but unable to locate. Do you have the regulation code # readily available? Lynette ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Will Chappell [chapcl@yahoo.com] Sent: Thursday, January 10, 2013 8:58 AM To: Cc: Histonet@Lists. Utsouthwestern. Edu Subject: Re: [Histonet] Medical Director credentials on reports Just passed CLIA. It is a CLIA requirement. Not sure about CAP. Will Chappell CHOC Children's Sent from my iPhone On Jan 10, 2013, at 5:45 AM, wrote: > Does anyone know, and maybe have the regs, whether either the Joint > Commission or the CAP *require* that the Medical Director's credentials be > included on the genlab and surgical reports? I have heard that it *is* a > requirement and now am hearing that it is *not* one. Clarification would be > nice! > > Example: > > Joe Smith > Medical Director > > OR > > Joe Smith, M.D. > Medical Director > > Thanks! > Michelle > ----- > No virus found in this message. > Checked by AVG - www.avg.com > Version: 2013.0.2805 / Virus Database: 2637/6021 - Release Date: 01/09/13 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From thigginsht <@t> msn.com Thu Jan 10 08:47:09 2013 From: thigginsht <@t> msn.com (Tim Higgins) Date: Thu Jan 10 08:47:13 2013 Subject: [Histonet] interview Message-ID: Gale and Pam, people on Histonet love to sit on their soap box and spew out ridiculous statements without any thought behind them at times. To say you need to "scale the volume down to a point where it is manageable" (tell that to your boss!! Whatever!!) is coming from someone who obviously has no working experience in the private sector. That might work at a research facility but I doubt it, or to say bluh bluh bluh something to do with peanuts and monkeys and the final one is, "The situation in histology will never get better otherwise", REALLY??? OJT is sometime a necessary route we as supervisor of labs that are experiencing a staffing shortage have to take to acquire the personnel we need to perform the work given. You have to do what you have to do, find someone to train if that is your only avenue. In a perfect world we would have qualified Histotechs knocking at our door every time there is an opening and an employer that is throwing money at us. Please give good sound advice, and stop the ridiculous remarks. It gets old real fast!! Good luck Gale!! Tim Message: 4 Date: Wed, 9 Jan 2013 18:37:36 +0000 (UTC) From: Pam Marcum Subject: Re: [Histonet] interview To: joelle weaver Cc: histonet@lists.utsouthwestern.edu, galel@unionhospital.org Message-ID: <117995865.193203.1357756656174.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Content-Type: text/plain; charset=utf-8 I totally understand hiring only experienced people however; I have a question.?? What do you do when you have no one available and the institute you work for will not help with moving expenses or sign on bonuses??? Believe me I know about training OJT today when you are shortstaffed and can't meet salary demands.?? Pam Marcum From epeters2 <@t> gmu.edu Thu Jan 10 09:30:10 2013 From: epeters2 <@t> gmu.edu (Esther C Peters) Date: Thu Jan 10 09:30:17 2013 Subject: [Histonet] RE: interview In-Reply-To: <41400FFE517878449D89114DD2526090087EA15BD2@tocmail1.tocad.orclinic.com> References: <41400FFE517878449D89114DD2526090087EA15BD2@tocmail1.tocad.orclinic.com> Message-ID: In my histology class for (mostly) college seniors, I now try to emphasize learning those specific qualities (follow directions, pay close attention to detail, and communicate well), as well as the subject. It is amazing how often, when you specify to "label only 4 structures" or "provide 2 complete sentences to explain" or "follow this format in a report," students will not do this! Esther C. Peters, Ph.D. Assistant Professor Department of Environmental Science & Policy Biology Program/Medical Technology Coordinator George Mason University 4400 University Drive, MSN 5F2 Fairfax, VA 22030-4444 ----- Original Message ----- From: "Kienitz, Kari" Date: Tuesday, January 8, 2013 4:10 pm Subject: [Histonet] RE: interview > Hi Gale, > > In my career I have had the pleasure of hiring many OJT > histotechs. With varying degrees of education and a whole range > of personalities. The things that tend to really stand out in the > candidates who excelled are the ones who could follow direction, > pay close attention to detail, and communicate well. Histology is > a field where as many mundane tasks must be done as the > complicated ones. > > For many years we would administer to all final candidates a > simple aptitude test. It was honestly something most 3rd graders > could do. Its was amazing how many people would get caught up > following directions on where to place their name on the paper and > simple math. > > > Kari Kienitz HT, (ASCP) > Histology Laboratory > Portland Gastroenterology > The Oregon Clinic > 1111 NE 99th Ave > Portland, OR 97220 > 503.935.8311 > kkienitz@orclinic.com > > > > > CONFIDENTIALITY WARNING: This e-mail and any attachments are for > the exclusive and confidential use of the intended recipient. If > you are not the intended recipient, please do not read, distribute > or take action in reliance upon this missive. If you have received > this in error, please notify the sender immediately by reply e- > mail and delete this message and its attachments from your > computer system. Thank you > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [histonet- > bounces@lists.utsouthwestern.edu] On Behalf Of Gale Limron > [GaleL@unionhospital.org]Sent: Tuesday, January 08, 2013 10:02 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] interview > > Hello, > I just found out today I will be doing 2nd interviews for 3 > candidates for a part time Histology position at our hospital on > Friday of this week. These candidates are not histotechs but are > willing to do online training and take ASCP board exam within 24 > months. I would appreciate some help with what questions to ask. I > did not attend the 1st interviews but these were done by our lab > manager who does not know a lot about what we do I > histology.............Thank you! > > Gale Limron CT,HT (ASCP) > Histology Supervisor > Union Hospital > 659 Boulevard > Dover, Ohio 44622 > 330-343-3311 ext 2562 > > > > This e-mail is intended only for the person or entity to which it > is addressed and may contain information that is privileged, > confidential or otherwise protected from disclosure. > Dissemination, distribution or copying of this e-mail or the > information herein by anyone other than the intended recipient, or > an employee or agent responsible for delivering the message to the > intended recipient, is prohibited. If you received this message in > error, please delete without copying and kindly e-mail a reply to > inform us of the mistake in > delivery._______________________________________________Histonet > mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Nagy_Natalie <@t> holyokehealth.com Thu Jan 10 09:36:00 2013 From: Nagy_Natalie <@t> holyokehealth.com (Natalie Nagy) Date: Thu Jan 10 09:36:20 2013 Subject: [Histonet] Help with CPT code 88363 for archived tissue retrieval Message-ID: <50EE99900200000F0033370C@hh11.holyokehealth.com> Hi everyone, I just have a question about CPT code 88363, first can it be used for pulling blocks for Oncotype DX testing, also is there a time limit on when this code can be used? Does it have to be within a year, a month, etc...of when the patient account went active? Thanks for all the help, Natalie J. Nagy (HT)ASCP Histology Supervisor Holyoke Medical Center CONFIDENTIALITY NOTICE: This email communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please reply to the sender immediately and destroy all copies of this communication and any attachments. For further information regarding Holyoke Medical Center's privacy policy, Please visit our Internet web site at http://www.holyokehealth.com From Joyce.Weems <@t> emoryhealthcare.org Thu Jan 10 09:40:40 2013 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Thu Jan 10 09:40:53 2013 Subject: [Histonet] Help with CPT code 88363 for archived tissue retrieval In-Reply-To: <50EE99900200000F0033370C@hh11.holyokehealth.com> References: <50EE99900200000F0033370C@hh11.holyokehealth.com> Message-ID: Yes, it is for review of material for molecular testing and can be added at any time as long as it is not ordered at the time the case is in process. If it is more than 30 days after the patient has been discharged, it is considered archived (according to Medicare) and we register it for a new acct number and bill it alone. Otherwise it is a late bill on current visit. We do this regardless if the patient is Medicare or not - just for consistency. I am not aware of any problems. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Natalie Nagy Sent: Thursday, January 10, 2013 10:36 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Help with CPT code 88363 for archived tissue retrieval Hi everyone, I just have a question about CPT code 88363, first can it be used for pulling blocks for Oncotype DX testing, also is there a time limit on when this code can be used? Does it have to be within a year, a month, etc...of when the patient account went active? Thanks for all the help, Natalie J. Nagy (HT)ASCP Histology Supervisor Holyoke Medical Center CONFIDENTIALITY NOTICE: This email communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please reply to the sender immediately and destroy all copies of this communication and any attachments. For further information regarding Holyoke Medical Center's privacy policy, Please visit our Internet web site at http://www.holyokehealth.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From billodonnell <@t> catholichealth.net Thu Jan 10 09:51:42 2013 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Thu Jan 10 09:52:01 2013 Subject: [Histonet] interview In-Reply-To: References: Message-ID: <4940DF6D1C5FDF48931B6966AAEF9395940CFF@chimsx08.CHI.catholichealth.net> OK, then, sound advice. (Hopefully) Assuming that a trained tech is out of the question: Hire someone with a minimum of an AS, but prefferably an MS. Pay scale should reflect the difference between a HS grad and a degreed person. This has been my policy for 17 years. MS or experience - otherwise, I do not even schedule an interview. Also, I believe it is grossly unfair to the new employee to limit their potential earnings at the start. Complete training, get 50 cents. Get your HT/HTL, get another dollar or two. (These are hourly increases, not the price of a stuffed bear that says "congratulations") Under that stipulation, seek within the system first. You have a better chance of gauging their work ethic and how well they get along with others and follow directions. If no luck within the system, go the local route - same requirements. How about contacting any nearby military bases, perhaps a spouse of a soldier is a tech and moving to town. It is not unusual for the bases personnel folks to know of such needs - but I admit, it is a long shot. Advertise nationaly - expensive! But it may unearth someone who will be planning on relocating to your area. This has happened once in my career - so it is not out of the realm of possibility. When interviewing an "unknown" your rescources for investigation are really pretty limited. Interview time is critical. Refrain from "tours" of the lab until after the initial interview. If they are not of hiring potential - don't waste your valuable time. Never promise what you cannot deliver. Remember that many people in today's economy are not working or are underemployed. Benefit packages are sometimes very valuable, especially to someone who hasn't had any for awhile. This is not a soapbox - though it would be a good place to get on one. This is the reality of our times. Excellent workers are available. Try to guage their desire, not for employment, but for learning new things. Ask questions that will guide them to give real answers as to how they might handle this or that "people" situation. Don't overlook the banal questions about hobbies or volunteer work. The answers can be very insightful. A person with no outside interests is a red flag as is someone with too many scattered interests. Don't look for common ground with these inqueries, but if you find it, ask yourself if your shared hobby is in anyway a plus for OJT work? As to paring down the work - this will be difficult but that is not the same as impossible. Work with medical staff to see what can be outsourced, even if for a short time. IHC? Some IHC? It is hard to find reference labs that do a lot of special special stains. Look at the data and see what special stains you offer that are hardly ever, or rarely requested. Talk with medical staff about eliminating these with the option of reintroducing them should the need increase. It's surprizing what a pathologist can do without! How much time are you spending in a day answering the phone? Pulling slides and filing? Cleaning processors and changing stainers? Chasing down missing info on your requisitions? Retreiving specimens? Could this be done by a part-time/full time clerk? Saving ten minutes here and 15 minutes there really does make a difference. Perhaps, if the clerk shows potential, they could become your OJT tech. I know that is how at least some of the fine techs who frequent this forum got into the field. Advantage - you see first hand their personality and productivity. None of these things are really new, but we do sometimes overlook them. It helps to have some objective fresh looks at the situation. Perhaps, bringing in an experienced per diem person can help you see areas that can be improved upon. After "X" number of years in the same place - we can get a bit myoptic. Maybe an histology consultant would be helpful. Again - fresh eyes and an open mind! Know that everything I just said is doable, but not easy. And every one or any one can fail or are not a good fit for your situation. Revenue may suffer, even if only temporary, but it simply may not be avoidable. And now - for a series of cliches that may actually be applicable: A burnt out tech is of no value to anyone, least of all, themselves. Nothing ventured, nothing gained. Two heads are better than one and might be twice as entertaining. Think outside the box (arrrgh - I can't believe I actually wrote that) Good luck in your endeavors and God bless! Thanks for indulging me. -Bill -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tim Higgins Sent: Thursday, January 10, 2013 8:47 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] interview Gale and Pam, people on Histonet love to sit on their soap box and spew out ridiculous statements without any thought behind them at times. To say you need to "scale the volume down to a point where it is manageable" (tell that to your boss!! Whatever!!) is coming from someone who obviously has no working experience in the private sector. That might work at a research facility but I doubt it, or to say bluh bluh bluh something to do with peanuts and monkeys and the final one is, "The situation in histology will never get better otherwise", REALLY??? OJT is sometime a necessary route we as supervisor of labs that are experiencing a staffing shortage have to take to acquire the personnel we need to perform the work given. You have to do what you have to do, find someone to train if that is your only avenue. In a perfect world we would have qualified Histotechs knocking at our door every time there is an opening and an employer that is throwing money at us. Please give good sound advice, and stop the ridiculous remarks. It gets old real fast!! Good luck Gale!! Tim Message: 4 Date: Wed, 9 Jan 2013 18:37:36 +0000 (UTC) From: Pam Marcum Subject: Re: [Histonet] interview To: joelle weaver Cc: histonet@lists.utsouthwestern.edu, galel@unionhospital.org Message-ID: <117995865.193203.1357756656174.JavaMail.root@sz0001a.westchester.pa.mai l.comcast.net> Content-Type: text/plain; charset=utf-8 I totally understand hiring only experienced people however; I have a question.?? What do you do when you have no one available and the institute you work for will not help with moving expenses or sign on bonuses??? Believe me I know about training OJT today when you are shortstaffed and can't meet salary demands.?? Pam Marcum _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. From joelleweaver <@t> hotmail.com Thu Jan 10 10:07:03 2013 From: joelleweaver <@t> hotmail.com (=?utf-8?B?am9lbGxld2VhdmVyQGhvdG1haWwuY29t?=) Date: Thu Jan 10 10:07:12 2013 Subject: =?utf-8?B?UmU6IFtIaXN0b25ldF0gaW50ZXJ2aWV3?= Message-ID: You can always delete if not interested Sent from my Verizon Wireless 4G LTE Smartphone ----- Reply message ----- From: "Tim Higgins" To: Subject: [Histonet] interview Date: Thu, Jan 10, 2013 8:47 am Gale and Pam, people on Histonet love to sit on their soap box and spew out ridiculous statements without any thought behind them at times. To say you need to "scale the volume down to a point where it is manageable" (tell that to your boss!! Whatever!!) is coming from someone who obviously has no working experience in the private sector. That might work at a research facility but I doubt it, or to say bluh bluh bluh something to do with peanuts and monkeys and the final one is, "The situation in histology will never get better otherwise", REALLY??? OJT is sometime a necessary route we as supervisor of labs that are experiencing a staffing shortage have to take to acquire the personnel we need to perform the work given. You have to do what you have to do, find someone to train if that is your only avenue. In a perfect world we would have qualified Histotechs knocking at our door every time there is an opening and an employer that is throwing money at us. Please give good sound advice, and stop the ridiculous remarks. It gets old real fast!! Good luck Gale!! Tim Message: 4 Date: Wed, 9 Jan 2013 18:37:36 +0000 (UTC) From: Pam Marcum Subject: Re: [Histonet] interview To: joelle weaver Cc: histonet@lists.utsouthwestern.edu, galel@unionhospital.org Message-ID: <117995865.193203.1357756656174.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Content-Type: text/plain; charset=utf-8 I totally understand hiring only experienced people however; I have a question.?? What do you do when you have no one available and the institute you work for will not help with moving expenses or sign on bonuses??? Believe me I know about training OJT today when you are shortstaffed and can't meet salary demands.?? Pam Marcum _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From paw555 <@t> yahoo.com Thu Jan 10 10:08:26 2013 From: paw555 <@t> yahoo.com (pam plumlee) Date: Thu Jan 10 10:08:34 2013 Subject: [Histonet] Her2 Staining Message-ID: <1357834106.50920.YahooMailNeo@web120906.mail.ne1.yahoo.com> Good morning Histonetters: ????Our CAP/ NY certified Cancer diagnostic reference lab is going to start to offer Her2 staining for breast and gastic tissues using the DAKO Kit on the bench.? The staining was validated by myself and our R&D group and the SOP will be transferred?to clinical.? I still am concerned about all the record keeping that should be done for CAP and would like to get feedback/help with this issue.? Please email or send contact info and I will call you.? Thanks, Pam in San Diego From rjbuesa <@t> yahoo.com Thu Jan 10 10:44:51 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jan 10 10:44:56 2013 Subject: [Histonet] interview In-Reply-To: References: Message-ID: <1357836291.29723.YahooMailNeo@web163103.mail.bf1.yahoo.com> Why, I wonder, every time an issue like this or similar surfaces in HistoNet it becomes a contentious issue? The original question was simple: a potential interviewer with little interviewing experience was about to interview 3 applicants without any histology experience for a histotech position. ? And that started?a torrent??of advises that?went from asking about histology even when that was impossible due to the fact that the interviewees were histology ignorants, to a series of unrealizable advises. One thing is training on the job (that was NOT the question) to many other suggestions having nothing to do with the original question and many "give and take" of answers/counter answers leading to Tim's request to "give advise, and stop the ridiculous remarks!" that I happen to agree with also. ? HistoNet is a very valuable resource but sometimes it becomes a place where some people want to impose their particular view points and that is not the?objective of this forum. ? Just receive the questions, if you think you can help with a sound and honest advise, please do it, but do not try to convince others that your view point is the "Gospel" or that you have to counter any other advise or opinion different to yours. ? This is not a "boxing ring" but a place where professionally originated answers are offered for the benefit of all, and especially for the benefit of those posting a problem. At least that is what I always try to do! Ren? J. From: "joelleweaver@hotmail.com" To: Tim Higgins ; histonet@lists.utsouthwestern.edu Sent: Thursday, January 10, 2013 11:07 AM Subject: Re: [Histonet] interview You can always delete if not interested Sent from my Verizon Wireless 4G LTE Smartphone ----- Reply message ----- From: "Tim Higgins" To: Subject: [Histonet] interview Date: Thu, Jan 10, 2013 8:47 am Gale and Pam, people on Histonet love to sit on their soap box and spew out ridiculous statements without any thought behind them at times.? To say you need to "scale the volume down to a point where it is manageable" (tell that to your boss!!? Whatever!!) is coming from someone who obviously has no working experience in the private sector.? That might work at a research facility but I doubt it, or to say bluh bluh bluh something to do with peanuts and monkeys and the final one is, "The situation in histology will never get better otherwise", REALLY???? OJT is sometime a necessary route we as supervisor of labs that are experiencing a staffing shortage have to take to acquire the personnel we need to perform the work given.? You have to do what you have to do, find someone to train if that is your only avenue.? In a perfect world we would have qualified Histotechs knocking at our door every time there is an opening and an employer that is throwing money at us. Please give good sound advice, and stop the ridiculous remarks.? It gets old real fast!! Good luck Gale!! Tim Message: 4 Date: Wed, 9 Jan 2013 18:37:36 +0000 (UTC) From: Pam? Marcum Subject: Re: [Histonet] interview To: joelle weaver Cc: histonet@lists.utsouthwestern.edu, galel@unionhospital.org Message-ID: ? ? ? ? <117995865.193203.1357756656174.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> ? ? ? ? Content-Type: text/plain; charset=utf-8 I totally understand hiring only experienced people however; I have a question.?? What do you do when you have no one available and the institute you work for will not help with moving expenses or sign on bonuses??? Believe me I know about training OJT today when you are shortstaffed and can't meet salary demands.?? Pam Marcum ??? ??? ??? ? ??? ??? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Thu Jan 10 11:05:30 2013 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Thu Jan 10 11:05:40 2013 Subject: [Histonet] FW: Announcing the 7th international retreat of applied IHC and molecular pathology (AIMP) Message-ID: <7C0A8F64A5774F35958075B7486936C3@prueggihctechlt> _____ From: Hadi Yaziji, MD [mailto:info@pathlearning.com] Sent: Thursday, January 10, 2013 9:55 AM To: Patsy Ruegg Subject: Announcing the 7th international retreat of applied IHC and molecular pathology (AIMP) Dear colleagues, It is our honor and pleasure to announce the 7th Annual International Retreat on Applied Immunohistochemistry and Molecular Pathology (AIMP), from February 3-7, 2013, in the sunny and beautiful Miami (Coral Gables), FL. The reason we have been offering this retreat for six years (and hopefully many more years to come) is because we maintained focus on the issues that matter the most to the practicing pathologist. In the way of example , the 7th annual retreat will continue to focus on the following aspects of IHC and molecular applications in our practice: 1. Among the >2000 new antibodies, can you offer a short list of new antibodies that I can benefit from their utility in surgical pathology? 2. What type of IHC platform should we bring to our pathology lab? 3. How can we effectively apply QA measures into our IHC lab and molecular lab? 4. What kind of impact do the ASCO/CAP guidelines have on our gross room workflow and documentation? 5. What do I need to do to ensure our lab is compliant with these guidelines, and compliant with other CAP and CLIA requirements? 6. What kind of FISH or molecular assays could our lab benefit from bringing in-house? 7. What are the most common molecular assays in oncologic pathology that I should be familiar with? 8. How do I build a molecular lab in my hospital? 9. Can you show me real - life examples of the staining pattern of antibodies I use in my practice? 10. What are the clinical applications of the molecular oncology assays, and what is the biology behind the testing and treatment? 11. What's the best IHC panel to properly classify lung cancer? 12. What's the best IHC panel to properly work up undifferentiated malignant neoplasm 13. What's the best IHC panel to properly work up metastatic carcinoma of unknown primary site? 13. What's the best IHC panel for mesothelioma vs. carcinoma? 14. How do I workup germ cell tumors by IHC? 15. How do I do IHC on cytology preparations? 16. What are the cancer predictive/prognostic assays that are not based on IHC, ISH or FISH? 17. What are the reimbursement issues facing payments for IHC, FISH and molecular assays? In essence, there is nothing that you deal with in your day-to-day surgical pathology practice as far as ancillary testing is concerned that is not going to be discussed at this retreat in some way, shape or form. We go straight to the bottom line without presenting to you any non-evidence based science that is not going to affect your practice of surgical pathology.? We constructed the educational program to address the above practical questions, as you can note from perusing the Schedule page. We assembled a group of the most experienced colleagues in the field, including Rich Cartun, Rich Eisen, David Hicks, Todd Barry, Jason Hornick, Savitri Krishnamurthy, and myself.? For the detailed schedule, please click here. On average, 40% of attendees are repeat attendees. Our colleagues who attended the first five courses truly enjoyed this event. In fact, many of them described it as "the most practical CME pathology course I've ever attended". We hope you will enjoy the upcoming course. It is very exciting and we look forward to seeing you there. Please? click here ? for detailed information of the program and how to register. This year, the ballroom can only accommodate a maximum of 175 participants in classroom seating. We recommend that you register fairly soon to guarantee a spot. Best regards, Hadi Yaziji, MD and Rich Eisen, MD Course Directors Unsubscribe Direct Mail for Mac This email is powered by Direct Mail for Mac. Learn More Report Spam From JMaslanka <@t> stpetes.org Thu Jan 10 11:21:45 2013 From: JMaslanka <@t> stpetes.org (JMaslanka@stpetes.org) Date: Thu Jan 10 11:22:24 2013 Subject: [Histonet] Block retention? Message-ID: I searched for retention policies and found what seems to be an 8 year difference in keeping blocks. How long do you retain your blocks? CDC website CLIA Sec. 493.1105 Standard: Retention requirements (7) Slide, block, and tissue retention-- (i) Slides. (ii) Blocks. Retain pathology specimen blocks for at least 2 years from the date of examination. CAP website states using CLIA as guide Surgical Pathology (including bone marrows) Wet tissue 2 weeks after final report Paraffin blocks 10 years Slides 10 years Reports 10 years Joe Maslanka BS, CT,HT (ASCP) Anatomical Pathology Technical Supervisor St Peter's Hospital,MT 59601 (P)(406) 447-2406 (F)(406)444-2126 Give thanks for ALL things..... "Kindness is the language the blind can see & the deaf can hear- Mark Twain From pruegg <@t> ihctech.net Thu Jan 10 11:22:31 2013 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Thu Jan 10 11:22:37 2013 Subject: [Histonet] Medical Director credentials on reports In-Reply-To: <001501cdef38$d2e80340$78b809c0$@imagesbyhopper.com> References: <001501cdef38$d2e80340$78b809c0$@imagesbyhopper.com> Message-ID: <1E03D84C7F7641F588D5C83ED7A35D02@prueggihctechlt> I have a question about this issue as well, does the medical director have the be an M.D. or can they be a PhD? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histotech@imagesbyhopper.com Sent: Thursday, January 10, 2013 6:46 AM To: 'Histonet@Lists. Utsouthwestern. Edu' Subject: [Histonet] Medical Director credentials on reports Does anyone know, and maybe have the regs, whether either the Joint Commission or the CAP *require* that the Medical Director's credentials be included on the genlab and surgical reports? I have heard that it *is* a requirement and now am hearing that it is *not* one. Clarification would be nice! Example: Joe Smith Medical Director OR Joe Smith, M.D. Medical Director Thanks! Michelle ----- No virus found in this message. Checked by AVG - www.avg.com Version: 2013.0.2805 / Virus Database: 2637/6021 - Release Date: 01/09/13 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Ken_Marissael <@t> vwr.com Thu Jan 10 11:35:09 2013 From: Ken_Marissael <@t> vwr.com (Ken_Marissael@vwr.com) Date: Thu Jan 10 11:35:15 2013 Subject: [Histonet] Ken Marissael is out of the office Message-ID: I will be out of the office starting 01/10/2013 and will not return until 01/17/2013. I will be away from 1/10 through 1/17 at the VWR national sales meeting. I will not have my computer for possibly several days, do if you have an emergency contact... healthcareservice.com or customer service at 877-881-1192. From histotech <@t> imagesbyhopper.com Thu Jan 10 11:38:58 2013 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Thu Jan 10 11:39:11 2013 Subject: [Histonet] Block retention? In-Reply-To: References: Message-ID: <003201cdef59$61b3e600$251bb200$@imagesbyhopper.com> We kept them for the most stringent of governing body requirements. We compared CAP, Joint Commission, CLIA and in our case the State of Florida. I have found that CAPs requirements were the most stringent. They cite 10 years on blocks, slides and reports. Michelle -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of JMaslanka@stpetes.org Sent: Thursday, January 10, 2013 12:22 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Block retention? I searched for retention policies and found what seems to be an 8 year difference in keeping blocks. How long do you retain your blocks? CDC website CLIA Sec. 493.1105 Standard: Retention requirements (7) Slide, block, and tissue retention-- (i) Slides. (ii) Blocks. Retain pathology specimen blocks for at least 2 years from the date of examination. CAP website states using CLIA as guide Surgical Pathology (including bone marrows) Wet tissue 2 weeks after final report Paraffin blocks 10 years Slides 10 years Reports 10 years Joe Maslanka BS, CT,HT (ASCP) Anatomical Pathology Technical Supervisor St Peter's Hospital,MT 59601 (P)(406) 447-2406 (F)(406)444-2126 Give thanks for ALL things..... "Kindness is the language the blind can see & the deaf can hear- Mark Twain _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----- No virus found in this message. Checked by AVG - www.avg.com Version: 2013.0.2805 / Virus Database: 2637/6021 - Release Date: 01/09/13 ----- No virus found in this message. Checked by AVG - www.avg.com Version: 2013.0.2805 / Virus Database: 2637/6021 - Release Date: 01/09/13 From wbenton <@t> cua.md Thu Jan 10 11:43:17 2013 From: wbenton <@t> cua.md (Walter Benton) Date: Thu Jan 10 11:43:25 2013 Subject: [Histonet] Block retention? In-Reply-To: References: Message-ID: <0B8979A204680A42B93A52B486088CD92CCF62B901@CUAEXH1.GCU-MD.local> Who inspects you? That is what you should follow unless your management wants to be more cautious and follow the CAP guidelines which you have listed. Entire procedure listed below. ANP.12500 Record Retention Phase II Surgical pathology records and materials are retained for an appropriate period. NOTE 1: Minimum requirements for surgical pathology, providing these are not less stringent than state and federal regulations, are: 1. Accession log records - 2 years 2. Wet tissue (stock bottle) - 2 weeks after final report 3. Paraffin blocks - 10 years (subject to Note 2, below) 4. Glass slides (including control slides) and reports - 10 years (slides must remain readable for this period) 5. Surgical pathology reports - 10 years (see Notes 3 and 4, below) 6. Fluorochrome-stained slides - at the discretion of the laboratory director 7. Fine needle aspiration slides - 10 years 8. Images of FISH studies - 10 years (see Note 5, below) There must be a documented policy for protecting and preserving the integrity and retrieval of surgical pathology materials and records.The retention period should be extended, when appropriate, to provide documentation for adequate quality control and medical care. NOTE 2: Regarding release of blocks for research purposes: Federal regulations require that a laboratory retain paraffin blocks for two years unless the tissue is blocked specifically for research and not used for patient diagnostic purposes.* The CLA requires, however, that paraffin blocks used for patient diagnostic purposes must be kept for at least 10 years. Nevertheless, such blocks may be released for research purposes after the two-year regulatory requirement if all of the following criteria are met: 1. For laboratories subject to US regulations, formal written authorization is obtained in accordance with the requirements of HIPAA if identifiable patient information is released. 2. The laboratory retains sufficient blocks to support the diagnosis for the full 10-year period. 3. Provision is made for retrieval by the laboratory of any blocks or material that remain after use in research, if the blocks or material are needed for diagnostic, legal, or other legitimate purposes. 4. The laboratory meets other relevant requirements including but not limited to the requirements of the institution, the directives of any applicable institutional review board (IRB) or similar entity; and state and local laws and regulations. NOTE 3: Pathology reports may be retained in either paper or electronic format. If retained in electronic format alone, however, the electronic reports must include a secure pathologist electronic signature. Images of paper reports--such as microfiche or PDF files--are acceptable. NOTE 4: Reports of outside consultations performed on cases from the laboratory (whether or not such consultation was requested by the laboratory) must be retained for 10 years after the date on which the original report was issued. NOTE 5: There is no retention requirement for images when the source slides remain readable for the required 10-year retention period. The 10-year retention requirement applies to images of slide preparations that are not readable for the 10-year period (e.g. FISH studies). Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 126 (All Deliveries to Suite 127) Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) wbenton@cua.md ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of JMaslanka@stpetes.org [JMaslanka@stpetes.org] Sent: Thursday, January 10, 2013 12:21 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Block retention? I searched for retention policies and found what seems to be an 8 year difference in keeping blocks. How long do you retain your blocks? CDC website CLIA Sec. 493.1105 Standard: Retention requirements (7) Slide, block, and tissue retention-- (i) Slides. (ii) Blocks. Retain pathology specimen blocks for at least 2 years from the date of examination. CAP website states using CLIA as guide Surgical Pathology (including bone marrows) Wet tissue 2 weeks after final report Paraffin blocks 10 years Slides 10 years Reports 10 years Joe Maslanka BS, CT,HT (ASCP) Anatomical Pathology Technical Supervisor St Peter's Hospital,MT 59601 (P)(406) 447-2406 (F)(406)444-2126 Give thanks for ALL things..... "Kindness is the language the blind can see & the deaf can hear- Mark Twain _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From thigginsht <@t> msn.com Thu Jan 10 11:53:38 2013 From: thigginsht <@t> msn.com (Tim Higgins) Date: Thu Jan 10 11:53:43 2013 Subject: [Histonet] interview In-Reply-To: <1357836291.29723.YahooMailNeo@web163103.mail.bf1.yahoo.com> References: , <1357836291.29723.YahooMailNeo@web163103.mail.bf1.yahoo.com> Message-ID: I will try and stay off my soap box while doing this. I was asked my opinion by one of the remark presenters that I obviously took issue with. Ok, as far as interviewing since that was the original question (thanks Rene for reminding me), I did get off topic. I too ask questions about special stains, fixation, processing, embedding, sectioning trying to hit all the areas, but usually in a way to view there hands-on knowledge while I show them around the lab. I also perform a embedding and section test for them, having them embed and cut tissue that I have personally placed in the cassette for their ability to be hard to cut and obtain good sections on. I also, give them small pieces of tissue and later melt it down to see how far they cut into in obtaining complete sections. Everyone has a way of doing things, no one way is right or better. Back to OJT!! Bill give some great advice, I search for someone I think will be a good fit and is excited to learn a trade that can serve them well in life. My experience with people with degrees is they usually want to be paid for the degree and not the work experience which is non when they are interviewing for a OJT job. I have had a lot of good employee who didn't have a degree yet but were working their way through. One lady I hired with a number of college credits but was not quite enough for her degree, she has since gotten her certification and is a manager at the job where I trained her (great job Stephanie!! Very proud of you!!) Don't be scared off by the OJT route, sure its a lot of work and time consuming but we have a lot of great Histotechs out there for went this route. All the ones that trained me were OJT and later grandfathered in to take the HTL test. No disrespect meant to anyone, just give good sound advice, get off the soap boxes, be helpful and maybe this forum will not be such a "boxing match" in the future. No back to work people!! Timothy N. Higgins, HT (ASCP), QIHC Date: Thu, 10 Jan 2013 08:44:51 -0800 From: rjbuesa@yahoo.com Subject: Re: [Histonet] interview To: joelleweaver@hotmail.com; thigginsht@msn.com; histonet@lists.utsouthwestern.edu Why, I wonder, every time an issue like this or similar surfaces in HistoNet it becomes a contentious issue? The original question was simple: a potential interviewer with little interviewing experience was about to interview 3 applicants without any histology experience for a histotech position. And that started a torrent of advises that went from asking about histology even when that was impossible due to the fact that the interviewees were histology ignorants, to a series of unrealizable advises. One thing is training on the job (that was NOT the question) to many other suggestions having nothing to do with the original question and many "give and take" of answers/counter answers leading to Tim's request to "give advise, and stop the ridiculous remarks!" that I happen to agree with also. HistoNet is a very valuable resource but sometimes it becomes a place where some people want to impose their particular view points and that is not the objective of this forum. Just receive the questions, if you think you can help with a sound and honest advise, please do it, but do not try to convince others that your view point is the "Gospel" or that you have to counter any other advise or opinion different to yours. This is not a "boxing ring" but a place where professionally originated answers are offered for the benefit of all, and especially for the benefit of those posting a problem. At least that is what I always try to do! Ren? J. From: "joelleweaver@hotmail.com" To: Tim Higgins ; histonet@lists.utsouthwestern.edu Sent: Thursday, January 10, 2013 11:07 AM Subject: Re: [Histonet] interview You can always delete if not interested Sent from my Verizon Wireless 4G LTE Smartphone ----- Reply message ----- From: "Tim Higgins" To: Subject: [Histonet] interview Date: Thu, Jan 10, 2013 8:47 am Gale and Pam, people on Histonet love to sit on their soap box and spew out ridiculous statements without any thought behind them at times. To say you need to "scale the volume down to a point where it is manageable" (tell that to your boss!! Whatever!!) is coming from someone who obviously has no working experience in the private sector. That might work at a research facility but I doubt it, or to say bluh bluh bluh something to do with peanuts and monkeys and the final one is, "The situation in histology will never get better otherwise", REALLY??? OJT is sometime a necessary route we as supervisor of labs that are experiencing a staffing shortage have to take to acquire the personnel we need to perform the work given. You have to do what you have to do, find someone to train if that is your only avenue. In a perfect world we would have qualified Histotechs knocking at our door every time there is an opening and an employer that is throwing money at us. Please give good sound advice, and stop the ridiculous remarks. It gets old real fast!! Good luck Gale!! Tim Message: 4 Date: Wed, 9 Jan 2013 18:37:36 +0000 (UTC) From: Pam Marcum Subject: Re: [Histonet] interview To: joelle weaver Cc: histonet@lists.utsouthwestern.edu, galel@unionhospital.org Message-ID: <117995865.193203.1357756656174.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Content-Type: text/plain; charset=utf-8 I totally understand hiring only experienced people however; I have a question.?? What do you do when you have no one available and the institute you work for will not help with moving expenses or sign on bonuses??? Believe me I know about training OJT today when you are shortstaffed and can't meet salary demands.?? Pam Marcum _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Erin.Martin <@t> ucsf.edu Thu Jan 10 12:13:19 2013 From: Erin.Martin <@t> ucsf.edu (Martin, Erin) Date: Thu Jan 10 12:13:42 2013 Subject: [Histonet] Xylene free processing Message-ID: <24B7B291CC88D04AB663958E77A1F59D14A262@ex09.net.ucsf.edu> Hi, We have been processing xylene free for a couple of years in ThermoFisher Excelsior processors. The tissue goes directly from isopropyl alcohol to paraffin. It works well for us. We sought out a xylene free protocol to reduce the amount of xylene in the lab. We do need to use xylene in the cleaning cycle but it's only a gallon. Good luck! Erin Erin Martin, Histology Supervisor UCSF Dermatopathology Service 415-353-7248 Confidentiality Notice The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential and/or priviledged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited. If you receive this in error, please contact the sender and delete the material from any computer. From cpyse <@t> x-celllab.com Thu Jan 10 12:17:55 2013 From: cpyse <@t> x-celllab.com (Cynthia Pyse) Date: Thu Jan 10 12:18:04 2013 Subject: [Histonet] her 2 neu, CISH vs FISH Message-ID: <005d01cdef5e$cfcff890$6f6fe9b0$@com> Good afternoon histonetters We are currently investigating bringing Her 2 Neu CISH staining into our lab. FISH staining has too many logistics nightmares for me to consider, not to mention the cost of purchasing digital scanning equipment. Our pathologist are off site in 5 different hospitals. Does anyone know if there is a sensitivity issue between fluoresce and chromogen staining? Are oncologists receptive to having the Her 2 Neu confirmed by chromogenic ISH as opposed to fluoresce ISH? Anyone performing Her 2 Neu by CISH, what, if any, issues have you encountered. Any help and information would be appreciated. Thanks in advance. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories 20 Northpointe Parkway Suite 100 Amherst, NY 14228 716-250-9235 etx. 232 e-mail cpyse@x-celllab.com From histotech <@t> imagesbyhopper.com Thu Jan 10 12:18:33 2013 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Thu Jan 10 12:18:48 2013 Subject: [Histonet] Medical Director credentials on reports In-Reply-To: <1E03D84C7F7641F588D5C83ED7A35D02@prueggihctechlt> References: <001501cdef38$d2e80340$78b809c0$@imagesbyhopper.com> <1E03D84C7F7641F588D5C83ED7A35D02@prueggihctechlt> Message-ID: <003301cdef5e$e9602910$bc207b30$@imagesbyhopper.com> Patsy, According to what I see from the CAP Lab Data Report for our facility, the Lab Director can be (MD, PhD, DO). Michelle -----Original Message----- From: Patsy Ruegg [mailto:pruegg@ihctech.net] Sent: Thursday, January 10, 2013 12:23 PM To: histotech@imagesbyhopper.com; 'Histonet@Lists. Utsouthwestern. Edu' Subject: RE: [Histonet] Medical Director credentials on reports I have a question about this issue as well, does the medical director have the be an M.D. or can they be a PhD? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histotech@imagesbyhopper.com Sent: Thursday, January 10, 2013 6:46 AM To: 'Histonet@Lists. Utsouthwestern. Edu' Subject: [Histonet] Medical Director credentials on reports Does anyone know, and maybe have the regs, whether either the Joint Commission or the CAP *require* that the Medical Director's credentials be included on the genlab and surgical reports? I have heard that it *is* a requirement and now am hearing that it is *not* one. Clarification would be nice! Example: Joe Smith Medical Director OR Joe Smith, M.D. Medical Director Thanks! Michelle ----- No virus found in this message. Checked by AVG - www.avg.com Version: 2013.0.2805 / Virus Database: 2637/6021 - Release Date: 01/09/13 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----- No virus found in this message. Checked by AVG - www.avg.com Version: 2013.0.2805 / Virus Database: 2637/6021 - Release Date: 01/09/13 ----- No virus found in this message. Checked by AVG - www.avg.com Version: 2013.0.2805 / Virus Database: 2637/6021 - Release Date: 01/09/13 From Joyce.Weems <@t> emoryhealthcare.org Thu Jan 10 12:33:10 2013 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Thu Jan 10 12:34:19 2013 Subject: [Histonet] Block retention? In-Reply-To: <003201cdef59$61b3e600$251bb200$@imagesbyhopper.com> References: <003201cdef59$61b3e600$251bb200$@imagesbyhopper.com> Message-ID: I wish CAP would revisit this. With all the molecular testing coming we will lose tissue that may be valuabe to a patient's treatment. I'm hanging on for dear life to what we have. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histotech@imagesbyhopper.com Sent: Thursday, January 10, 2013 12:39 PM To: JMaslanka@stpetes.org; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Block retention? We kept them for the most stringent of governing body requirements. We compared CAP, Joint Commission, CLIA and in our case the State of Florida. I have found that CAPs requirements were the most stringent. They cite 10 years on blocks, slides and reports. Michelle -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of JMaslanka@stpetes.org Sent: Thursday, January 10, 2013 12:22 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Block retention? I searched for retention policies and found what seems to be an 8 year difference in keeping blocks. How long do you retain your blocks? CDC website CLIA Sec. 493.1105 Standard: Retention requirements (7) Slide, block, and tissue retention-- (i) Slides. (ii) Blocks. Retain pathology specimen blocks for at least 2 years from the date of examination. CAP website states using CLIA as guide Surgical Pathology (including bone marrows) Wet tissue 2 weeks after final report Paraffin blocks 10 years Slides 10 years Reports 10 years Joe Maslanka BS, CT,HT (ASCP) Anatomical Pathology Technical Supervisor St Peter's Hospital,MT 59601 (P)(406) 447-2406 (F)(406)444-2126 Give thanks for ALL things..... "Kindness is the language the blind can see & the deaf can hear- Mark Twain _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----- No virus found in this message. Checked by AVG - www.avg.com Version: 2013.0.2805 / Virus Database: 2637/6021 - Release Date: 01/09/13 ----- No virus found in this message. Checked by AVG - www.avg.com Version: 2013.0.2805 / Virus Database: 2637/6021 - Release Date: 01/09/13 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From rob <@t> foliobio.com Thu Jan 10 12:49:25 2013 From: rob <@t> foliobio.com (Rob Day) Date: Thu Jan 10 12:49:43 2013 Subject: [Histonet] Block retention? In-Reply-To: References: <003201cdef59$61b3e600$251bb200$@imagesbyhopper.com> Message-ID: <155AB861-C32A-427B-B757-7019C5F32BFA@foliobio.com> Your legal office will probably advise you NOT to retain specimens any longer than is absolutely necessary. You could always choose to de-identify older samples and donate them to a commercial biobank, that way you can be sure that the maximum possible research value will be extracted from them. Contact me if you want suggestions of who to donate to. Rob Day. On Jan 10, 2013, at 1:33 PM, "Weems, Joyce K." wrote: > I wish CAP would revisit this. With all the molecular testing coming we will lose tissue that may be valuabe to a patient's treatment. I'm hanging on for dear life to what we have. > > Joyce Weems > Pathology Manager > 678-843-7376 Phone > 678-843-7831 Fax > joyce.weems@emoryhealthcare.org > > > > www.saintjosephsatlanta.org > 5665 Peachtree Dunwoody Road > Atlanta, GA 30342 > > This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histotech@imagesbyhopper.com > Sent: Thursday, January 10, 2013 12:39 PM > To: JMaslanka@stpetes.org; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Block retention? > > We kept them for the most stringent of governing body requirements. We compared CAP, Joint Commission, CLIA and in our case the State of Florida. > I have found that CAPs requirements were the most stringent. They cite 10 years on blocks, slides and reports. > > Michelle > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of JMaslanka@stpetes.org > Sent: Thursday, January 10, 2013 12:22 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Block retention? > > I searched for retention policies and found what seems to be an 8 year difference in keeping blocks. How long do you retain your blocks? > > > CDC website CLIA > Sec. 493.1105 Standard: Retention requirements > > > > > > > > (7) Slide, block, and tissue retention-- > (i) Slides. > > (ii) Blocks. Retain pathology specimen blocks for at least 2 years from the date of examination. > > > > > > > > > > > > CAP website states using CLIA as guide > > > > > > > Surgical Pathology (including bone marrows) > Wet tissue > 2 weeks after final report > Paraffin blocks > 10 years > Slides > 10 years > Reports > 10 years > > > > > > > > > > > > > > > > > > > > > > > Joe Maslanka BS, CT,HT (ASCP) > Anatomical Pathology Technical Supervisor > St Peter's Hospital,MT 59601 > (P)(406) 447-2406 > (F)(406)444-2126 > > Give thanks for ALL things..... > "Kindness is the language the blind can see & the deaf can hear- Mark > Twain > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ----- > No virus found in this message. > Checked by AVG - www.avg.com > Version: 2013.0.2805 / Virus Database: 2637/6021 - Release Date: 01/09/13 > ----- > No virus found in this message. > Checked by AVG - www.avg.com > Version: 2013.0.2805 / Virus Database: 2637/6021 - Release Date: 01/09/13 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ________________________________ > > This e-mail message (including any attachments) is for the sole use of > the intended recipient(s) and may contain confidential and privileged > information. If the reader of this message is not the intended > recipient, you are hereby notified that any dissemination, distribution > or copying of this message (including any attachments) is strictly > prohibited. > > If you have received this message in error, please contact > the sender by reply e-mail message and destroy all copies of the > original message (including attachments). > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet Rob Day Business Development Folio Biosciences 1476 Manning Pkwy, Powell, Ohio 43065 Direct Line: (614) 407-4547 | Main Office Phone: (614) 846-2809 | Fax: (877) 591-1815 skype: invasifspecies http://foliobio.com www.linkedin.com/in/robdaybiotech This message, including any attachments, is confidential and may be privileged or may contain health information protected by state and federal law. Information and opinions expressed in this message and/or attachments are those of the author and are not necessarily those of the company. If you are not the intended recipient, please notify the sender and delete this message from your system. Any use of this information by individuals other than the intended recipient is strictly prohibited. From marktarango <@t> gmail.com Thu Jan 10 13:00:47 2013 From: marktarango <@t> gmail.com (Mark Tarango) Date: Thu Jan 10 13:00:52 2013 Subject: [Histonet] Help with CPT code 88363 for archived tissue retrieval In-Reply-To: <50EE99900200000F0033370C@hh11.holyokehealth.com> References: <50EE99900200000F0033370C@hh11.holyokehealth.com> Message-ID: It can't be used to just pull blocks. The slides have to be reviewed and the best block chosen by a pathologist. If there is only one block then the pathologist needs to look at the slides and determine if there is enough tissue for molecular testing. It's a professional charge. Use it on re-accessioned cases for which molecular testing is requested and the best block needs to be chosen. Yes we use it when sending out for Oncotype DX if the case was signed out over 30 days before. Mark On Thu, Jan 10, 2013 at 7:36 AM, Natalie Nagy < Nagy_Natalie@holyokehealth.com> wrote: > Hi everyone, > I just have a question about CPT code 88363, first can > it be used for pulling blocks for Oncotype DX testing, also is there a time > limit on when this code can be used? Does it have to be within a year, a > month, etc...of when the patient account went active? > > Thanks for all the help, > > Natalie J. Nagy (HT)ASCP > Histology Supervisor > Holyoke Medical Center > > > CONFIDENTIALITY NOTICE: This email communication and any attachments may > contain confidential and privileged information for the use of the > designated recipients named above. If you are not the intended recipient, > you are hereby notified that you have received this communication in error > and that any review, disclosure, dissemination, distribution or copying of > it or its contents is prohibited. If you have received this communication > in error, please reply to the sender immediately and destroy all copies of > this communication and any attachments. For further information regarding > Holyoke Medical Center's privacy policy, Please visit our Internet web site > at http://www.holyokehealth.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Joyce.Weems <@t> emoryhealthcare.org Thu Jan 10 13:04:20 2013 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Thu Jan 10 13:04:29 2013 Subject: [Histonet] Help with CPT code 88363 for archived tissue retrieval In-Reply-To: References: <50EE99900200000F0033370C@hh11.holyokehealth.com> Message-ID: It is also a technical charge, as well. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Tarango Sent: Thursday, January 10, 2013 2:01 PM To: Natalie Nagy Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Help with CPT code 88363 for archived tissue retrieval It can't be used to just pull blocks. The slides have to be reviewed and the best block chosen by a pathologist. If there is only one block then the pathologist needs to look at the slides and determine if there is enough tissue for molecular testing. It's a professional charge. Use it on re-accessioned cases for which molecular testing is requested and the best block needs to be chosen. Yes we use it when sending out for Oncotype DX if the case was signed out over 30 days before. Mark On Thu, Jan 10, 2013 at 7:36 AM, Natalie Nagy < Nagy_Natalie@holyokehealth.com> wrote: > Hi everyone, > I just have a question about CPT code 88363, first > can it be used for pulling blocks for Oncotype DX testing, also is > there a time limit on when this code can be used? Does it have to be > within a year, a month, etc...of when the patient account went active? > > Thanks for all the help, > > Natalie J. Nagy (HT)ASCP > Histology Supervisor > Holyoke Medical Center > > > CONFIDENTIALITY NOTICE: This email communication and any attachments > may contain confidential and privileged information for the use of the > designated recipients named above. If you are not the intended > recipient, you are hereby notified that you have received this > communication in error and that any review, disclosure, dissemination, > distribution or copying of it or its contents is prohibited. If you > have received this communication in error, please reply to the sender > immediately and destroy all copies of this communication and any > attachments. For further information regarding Holyoke Medical > Center's privacy policy, Please visit our Internet web site at > http://www.holyokehealth.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From Joyce.Weems <@t> emoryhealthcare.org Thu Jan 10 13:05:53 2013 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Thu Jan 10 13:06:02 2013 Subject: [Histonet] Help with CPT code 88363 for archived tissue retrieval In-Reply-To: References: <50EE99900200000F0033370C@hh11.holyokehealth.com> Message-ID: And I should explain the reason I most know this.. our pathologists were denied because the tech charge hadn't been entered yet. So now I make sure the tech charge is entered before sending to the pathologists billing folks. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Tarango Sent: Thursday, January 10, 2013 2:01 PM To: Natalie Nagy Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Help with CPT code 88363 for archived tissue retrieval It can't be used to just pull blocks. The slides have to be reviewed and the best block chosen by a pathologist. If there is only one block then the pathologist needs to look at the slides and determine if there is enough tissue for molecular testing. It's a professional charge. Use it on re-accessioned cases for which molecular testing is requested and the best block needs to be chosen. Yes we use it when sending out for Oncotype DX if the case was signed out over 30 days before. Mark On Thu, Jan 10, 2013 at 7:36 AM, Natalie Nagy < Nagy_Natalie@holyokehealth.com> wrote: > Hi everyone, > I just have a question about CPT code 88363, first > can it be used for pulling blocks for Oncotype DX testing, also is > there a time limit on when this code can be used? Does it have to be > within a year, a month, etc...of when the patient account went active? > > Thanks for all the help, > > Natalie J. Nagy (HT)ASCP > Histology Supervisor > Holyoke Medical Center > > > CONFIDENTIALITY NOTICE: This email communication and any attachments > may contain confidential and privileged information for the use of the > designated recipients named above. If you are not the intended > recipient, you are hereby notified that you have received this > communication in error and that any review, disclosure, dissemination, > distribution or copying of it or its contents is prohibited. If you > have received this communication in error, please reply to the sender > immediately and destroy all copies of this communication and any > attachments. For further information regarding Holyoke Medical > Center's privacy policy, Please visit our Internet web site at > http://www.holyokehealth.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From mpence <@t> grhs.net Thu Jan 10 13:27:54 2013 From: mpence <@t> grhs.net (Mike Pence) Date: Thu Jan 10 13:28:31 2013 Subject: [Histonet] Help with CPT code 88363 for archived tissue retrieval In-Reply-To: Message-ID: <661949901A768E4F9CC16D8AF8F2838C03974F73@is-e2k3.grhs.net> So are you putting the charge thru twice or is the charge for the 88363 divided out into tech and prof.? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Thursday, January 10, 2013 1:06 PM To: 'Mark Tarango'; Natalie Nagy Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Help with CPT code 88363 for archived tissue retrieval And I should explain the reason I most know this.. our pathologists were denied because the tech charge hadn't been entered yet. So now I make sure the tech charge is entered before sending to the pathologists billing folks. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Tarango Sent: Thursday, January 10, 2013 2:01 PM To: Natalie Nagy Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Help with CPT code 88363 for archived tissue retrieval It can't be used to just pull blocks. The slides have to be reviewed and the best block chosen by a pathologist. If there is only one block then the pathologist needs to look at the slides and determine if there is enough tissue for molecular testing. It's a professional charge. Use it on re-accessioned cases for which molecular testing is requested and the best block needs to be chosen. Yes we use it when sending out for Oncotype DX if the case was signed out over 30 days before. Mark On Thu, Jan 10, 2013 at 7:36 AM, Natalie Nagy < Nagy_Natalie@holyokehealth.com> wrote: > Hi everyone, > I just have a question about CPT code 88363, first > can it be used for pulling blocks for Oncotype DX testing, also is > there a time limit on when this code can be used? Does it have to be > within a year, a month, etc...of when the patient account went active? > > Thanks for all the help, > > Natalie J. Nagy (HT)ASCP > Histology Supervisor > Holyoke Medical Center > > > CONFIDENTIALITY NOTICE: This email communication and any attachments > may contain confidential and privileged information for the use of the > designated recipients named above. If you are not the intended > recipient, you are hereby notified that you have received this > communication in error and that any review, disclosure, dissemination, > distribution or copying of it or its contents is prohibited. If you > have received this communication in error, please reply to the sender > immediately and destroy all copies of this communication and any > attachments. For further information regarding Holyoke Medical > Center's privacy policy, Please visit our Internet web site at > http://www.holyokehealth.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Joyce.Weems <@t> emoryhealthcare.org Thu Jan 10 13:34:33 2013 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Thu Jan 10 13:34:50 2013 Subject: [Histonet] Help with CPT code 88363 for archived tissue retrieval In-Reply-To: <661949901A768E4F9CC16D8AF8F2838C03974F73@is-e2k3.grhs.net> References: <661949901A768E4F9CC16D8AF8F2838C03974F73@is-e2k3.grhs.net> Message-ID: Divided - but can be billed globally if that is how you bill. The professional uses the 26 modifier. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: Mike Pence [mailto:mpence@grhs.net] Sent: Thursday, January 10, 2013 2:28 PM To: Weems, Joyce K.; Mark Tarango; Natalie Nagy Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Help with CPT code 88363 for archived tissue retrieval So are you putting the charge thru twice or is the charge for the 88363 divided out into tech and prof.? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Thursday, January 10, 2013 1:06 PM To: 'Mark Tarango'; Natalie Nagy Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Help with CPT code 88363 for archived tissue retrieval And I should explain the reason I most know this.. our pathologists were denied because the tech charge hadn't been entered yet. So now I make sure the tech charge is entered before sending to the pathologists billing folks. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Tarango Sent: Thursday, January 10, 2013 2:01 PM To: Natalie Nagy Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Help with CPT code 88363 for archived tissue retrieval It can't be used to just pull blocks. The slides have to be reviewed and the best block chosen by a pathologist. If there is only one block then the pathologist needs to look at the slides and determine if there is enough tissue for molecular testing. It's a professional charge. Use it on re-accessioned cases for which molecular testing is requested and the best block needs to be chosen. Yes we use it when sending out for Oncotype DX if the case was signed out over 30 days before. Mark On Thu, Jan 10, 2013 at 7:36 AM, Natalie Nagy < Nagy_Natalie@holyokehealth.com> wrote: > Hi everyone, > I just have a question about CPT code 88363, first > can it be used for pulling blocks for Oncotype DX testing, also is > there a time limit on when this code can be used? Does it have to be > within a year, a month, etc...of when the patient account went active? > > Thanks for all the help, > > Natalie J. Nagy (HT)ASCP > Histology Supervisor > Holyoke Medical Center > > > CONFIDENTIALITY NOTICE: This email communication and any attachments > may contain confidential and privileged information for the use of the > designated recipients named above. If you are not the intended > recipient, you are hereby notified that you have received this > communication in error and that any review, disclosure, dissemination, > distribution or copying of it or its contents is prohibited. If you > have received this communication in error, please reply to the sender > immediately and destroy all copies of this communication and any > attachments. For further information regarding Holyoke Medical > Center's privacy policy, Please visit our Internet web site at > http://www.holyokehealth.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Thu Jan 10 13:46:46 2013 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Thu Jan 10 13:46:58 2013 Subject: [Histonet] Help with CPT code 88363 for archived tissue retrieval In-Reply-To: <661949901A768E4F9CC16D8AF8F2838C03974F73@is-e2k3.grhs.net> References: <661949901A768E4F9CC16D8AF8F2838C03974F73@is-e2k3.grhs.net> Message-ID: <50EED456.7400.0077.1@harthosp.org> I'm not 100% sure, but I don't think this CPT code has a "Technical" component. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-2204 Fax >>> "Mike Pence" 1/10/2013 2:27 PM >>> So are you putting the charge thru twice or is the charge for the 88363 divided out into tech and prof.? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Thursday, January 10, 2013 1:06 PM To: 'Mark Tarango'; Natalie Nagy Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Help with CPT code 88363 for archived tissue retrieval And I should explain the reason I most know this.. our pathologists were denied because the tech charge hadn't been entered yet. So now I make sure the tech charge is entered before sending to the pathologists billing folks. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Tarango Sent: Thursday, January 10, 2013 2:01 PM To: Natalie Nagy Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Help with CPT code 88363 for archived tissue retrieval It can't be used to just pull blocks. The slides have to be reviewed and the best block chosen by a pathologist. If there is only one block then the pathologist needs to look at the slides and determine if there is enough tissue for molecular testing. It's a professional charge. Use it on re-accessioned cases for which molecular testing is requested and the best block needs to be chosen. Yes we use it when sending out for Oncotype DX if the case was signed out over 30 days before. Mark On Thu, Jan 10, 2013 at 7:36 AM, Natalie Nagy < Nagy_Natalie@holyokehealth.com> wrote: > Hi everyone, > I just have a question about CPT code 88363, first > can it be used for pulling blocks for Oncotype DX testing, also is > there a time limit on when this code can be used? Does it have to be > within a year, a month, etc...of when the patient account went active? > > Thanks for all the help, > > Natalie J. Nagy (HT)ASCP > Histology Supervisor > Holyoke Medical Center > > > CONFIDENTIALITY NOTICE: This email communication and any attachments > may contain confidential and privileged information for the use of the > designated recipients named above. If you are not the intended > recipient, you are hereby notified that you have received this > communication in error and that any review, disclosure, dissemination, > distribution or copying of it or its contents is prohibited. If you > have received this communication in error, please reply to the sender > immediately and destroy all copies of this communication and any > attachments. For further information regarding Holyoke Medical > Center's privacy policy, Please visit our Internet web site at > http://www.holyokehealth.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Lynne.Bell <@t> cvmc.org Thu Jan 10 13:50:18 2013 From: Lynne.Bell <@t> cvmc.org (Bell, Lynne) Date: Thu Jan 10 13:50:24 2013 Subject: [Histonet] Help with CPT code 88363 for archived tissue retrieval In-Reply-To: <50EED456.7400.0077.1@harthosp.org> References: <661949901A768E4F9CC16D8AF8F2838C03974F73@is-e2k3.grhs.net> <50EED456.7400.0077.1@harthosp.org> Message-ID: I agree, Dr. Cartun. I believe that the CPT Coding book specifically says that it is only a professional charge. Lynne Bell, HT (ASCP) Histology Team Leader Central Vermont Medical Center 130 Fisher Road Berlin, VT 05641 802-371-4923 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Thursday, January 10, 2013 2:47 PM To: Joyce K. Weems; Mark Tarango; Mike Pence; Natalie Nagy Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Help with CPT code 88363 for archived tissue retrieval I'm not 100% sure, but I don't think this CPT code has a "Technical" component. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-2204 Fax >>> "Mike Pence" 1/10/2013 2:27 PM >>> So are you putting the charge thru twice or is the charge for the 88363 divided out into tech and prof.? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Thursday, January 10, 2013 1:06 PM To: 'Mark Tarango'; Natalie Nagy Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Help with CPT code 88363 for archived tissue retrieval And I should explain the reason I most know this.. our pathologists were denied because the tech charge hadn't been entered yet. So now I make sure the tech charge is entered before sending to the pathologists billing folks. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Tarango Sent: Thursday, January 10, 2013 2:01 PM To: Natalie Nagy Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Help with CPT code 88363 for archived tissue retrieval It can't be used to just pull blocks. The slides have to be reviewed and the best block chosen by a pathologist. If there is only one block then the pathologist needs to look at the slides and determine if there is enough tissue for molecular testing. It's a professional charge. Use it on re-accessioned cases for which molecular testing is requested and the best block needs to be chosen. Yes we use it when sending out for Oncotype DX if the case was signed out over 30 days before. Mark On Thu, Jan 10, 2013 at 7:36 AM, Natalie Nagy < Nagy_Natalie@holyokehealth.com> wrote: > Hi everyone, > I just have a question about CPT code 88363, first > can it be used for pulling blocks for Oncotype DX testing, also is > there a time limit on when this code can be used? Does it have to be > within a year, a month, etc...of when the patient account went active? > > Thanks for all the help, > > Natalie J. Nagy (HT)ASCP > Histology Supervisor > Holyoke Medical Center > > > CONFIDENTIALITY NOTICE: This email communication and any attachments > may contain confidential and privileged information for the use of the > designated recipients named above. If you are not the intended > recipient, you are hereby notified that you have received this > communication in error and that any review, disclosure, dissemination, > distribution or copying of it or its contents is prohibited. If you > have received this communication in error, please reply to the sender > immediately and destroy all copies of this communication and any > attachments. For further information regarding Holyoke Medical > Center's privacy policy, Please visit our Internet web site at > http://www.holyokehealth.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Jan 10 14:10:58 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jan 10 14:11:03 2013 Subject: [Histonet] Xylene free processing In-Reply-To: <24B7B291CC88D04AB663958E77A1F59D14A262@ex09.net.ucsf.edu> References: <24B7B291CC88D04AB663958E77A1F59D14A262@ex09.net.ucsf.edu> Message-ID: <1357848658.75870.YahooMailNeo@web163103.mail.bf1.yahoo.com> Not even to clean the tissue processor you will need xylene if you prepare a 5% solution of liquid dish washer soap in isopropyl alcohol. Place this solution in the cleaning "used xylene" container and clean the tissue processor with it. Ren? J. From: "Martin, Erin" To: histonet Sent: Thursday, January 10, 2013 1:13 PM Subject: [Histonet] Xylene free processing Hi, We have been processing xylene free for a couple of years in ThermoFisher Excelsior processors.? The tissue goes directly from isopropyl alcohol to paraffin.? It works well for us.? We sought out a xylene free protocol to reduce the amount of xylene in the lab. We do need to use xylene in the cleaning cycle but it's only a gallon. Good luck! Erin Erin Martin, Histology Supervisor UCSF? Dermatopathology Service 415-353-7248 Confidentiality Notice The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential and/or priviledged material.? Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited.? If you receive this in error, please contact the sender and delete the material from any computer. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Jan 10 14:13:14 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jan 10 14:13:19 2013 Subject: [Histonet] Block retention? In-Reply-To: References: Message-ID: <1357848794.42686.YahooMailNeo@web163102.mail.bf1.yahoo.com> Retention time will depend on the regulations in your state. In Florida we retain blocks for 9 years. Ren? J. From: "JMaslanka@stpetes.org" To: histonet@lists.utsouthwestern.edu Sent: Thursday, January 10, 2013 12:21 PM Subject: [Histonet] Block retention? I searched for retention policies and found what seems to be an 8 year difference in keeping blocks. How long do you retain your blocks? CDC website CLIA Sec. 493.1105? Standard: Retention requirements ? ? (7) Slide, block, and tissue retention-- ? ? (i) Slides. ? ? (ii) Blocks. Retain pathology specimen blocks for at least 2 years from the date of examination. CAP website states using CLIA as guide Surgical Pathology (including bone marrows) Wet tissue 2 weeks after final report Paraffin blocks 10 years Slides 10 years Reports 10 years Joe Maslanka BS, CT,HT (ASCP) Anatomical Pathology Technical Supervisor St Peter's Hospital,MT 59601 (P)(406) 447-2406 (F)(406)444-2126 Give thanks for ALL things..... "Kindness is the language the blind can see & the deaf can hear- Mark Twain _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Joyce.Weems <@t> emoryhealthcare.org Thu Jan 10 14:17:44 2013 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Thu Jan 10 14:18:08 2013 Subject: [Histonet] Help with CPT code 88363 for archived tissue retrieval In-Reply-To: <50EED456.7400.0077.1@harthosp.org> References: <661949901A768E4F9CC16D8AF8F2838C03974F73@is-e2k3.grhs.net> <50EED456.7400.0077.1@harthosp.org> Message-ID: Maybe so, but I am getting reimbursed for tech only. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: Richard Cartun [mailto:Rcartun@harthosp.org] Sent: Thursday, January 10, 2013 2:47 PM To: Weems, Joyce K.; Mark Tarango; Mike Pence; Natalie Nagy Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Help with CPT code 88363 for archived tissue retrieval I'm not 100% sure, but I don't think this CPT code has a "Technical" component. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-2204 Fax >>> "Mike Pence" 1/10/2013 2:27 PM >>> So are you putting the charge thru twice or is the charge for the 88363 divided out into tech and prof.? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Thursday, January 10, 2013 1:06 PM To: 'Mark Tarango'; Natalie Nagy Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Help with CPT code 88363 for archived tissue retrieval And I should explain the reason I most know this.. our pathologists were denied because the tech charge hadn't been entered yet. So now I make sure the tech charge is entered before sending to the pathologists billing folks. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Tarango Sent: Thursday, January 10, 2013 2:01 PM To: Natalie Nagy Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Help with CPT code 88363 for archived tissue retrieval It can't be used to just pull blocks. The slides have to be reviewed and the best block chosen by a pathologist. If there is only one block then the pathologist needs to look at the slides and determine if there is enough tissue for molecular testing. It's a professional charge. Use it on re-accessioned cases for which molecular testing is requested and the best block needs to be chosen. Yes we use it when sending out for Oncotype DX if the case was signed out over 30 days before. Mark On Thu, Jan 10, 2013 at 7:36 AM, Natalie Nagy < Nagy_Natalie@holyokehealth.com> wrote: > Hi everyone, > I just have a question about CPT code 88363, first > can it be used for pulling blocks for Oncotype DX testing, also is > there a time limit on when this code can be used? Does it have to be > within a year, a month, etc...of when the patient account went active? > > Thanks for all the help, > > Natalie J. Nagy (HT)ASCP > Histology Supervisor > Holyoke Medical Center > > > CONFIDENTIALITY NOTICE: This email communication and any attachments > may contain confidential and privileged information for the use of the > designated recipients named above. If you are not the intended > recipient, you are hereby notified that you have received this > communication in error and that any review, disclosure, dissemination, > distribution or copying of it or its contents is prohibited. If you > have received this communication in error, please reply to the sender > immediately and destroy all copies of this communication and any > attachments. For further information regarding Holyoke Medical > Center's privacy policy, Please visit our Internet web site at > http://www.holyokehealth.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------- This message was secured by ZixCorp(R). From cforster <@t> umn.edu Thu Jan 10 15:16:32 2013 From: cforster <@t> umn.edu (Colleen Forster) Date: Thu Jan 10 15:16:37 2013 Subject: [Histonet] zebra fish experts...... Message-ID: <50EF2FB0.6030908@umn.edu> Anyone out there doing zebra fish work I need your help! We are trying to fix zebra fish kidneys in 10% formalin and then freeze...the samples just seem to crumble when we try to cut...can someone help me out here with fixing/freezing methods for these little buggers!! Thanks in advance!! Colleen Forster U of MN From vtobias <@t> uw.edu Thu Jan 10 16:46:55 2013 From: vtobias <@t> uw.edu (Victor A. Tobias) Date: Thu Jan 10 16:47:25 2013 Subject: [Histonet] Help with CPT code 88363 for archived tissue retrieval In-Reply-To: References: <661949901A768E4F9CC16D8AF8F2838C03974F73@is-e2k3.grhs.net> <50EED456.7400.0077.1@harthosp.org> Message-ID: We have been billing a tech and pro fee since 2011. Whether we are getting reimbursed for both is another question. Victor Victor Tobias HT(ASCP) Clinical Applications Analyst Harborview Medical Center Dept of Pathology Room NJB244 Seattle, WA 98104 vtobias@u.washington.edu 206-744-2735 206-744-8240 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bell, Lynne Sent: Thursday, January 10, 2013 11:50 AM To: 'Richard Cartun'; Joyce K. Weems; Mark Tarango; Mike Pence; Natalie Nagy Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Help with CPT code 88363 for archived tissue retrieval I agree, Dr. Cartun. I believe that the CPT Coding book specifically says that it is only a professional charge. Lynne Bell, HT (ASCP) Histology Team Leader Central Vermont Medical Center 130 Fisher Road Berlin, VT 05641 802-371-4923 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Thursday, January 10, 2013 2:47 PM To: Joyce K. Weems; Mark Tarango; Mike Pence; Natalie Nagy Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Help with CPT code 88363 for archived tissue retrieval I'm not 100% sure, but I don't think this CPT code has a "Technical" component. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-2204 Fax >>> "Mike Pence" 1/10/2013 2:27 PM >>> So are you putting the charge thru twice or is the charge for the 88363 divided out into tech and prof.? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Thursday, January 10, 2013 1:06 PM To: 'Mark Tarango'; Natalie Nagy Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Help with CPT code 88363 for archived tissue retrieval And I should explain the reason I most know this.. our pathologists were denied because the tech charge hadn't been entered yet. So now I make sure the tech charge is entered before sending to the pathologists billing folks. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Tarango Sent: Thursday, January 10, 2013 2:01 PM To: Natalie Nagy Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Help with CPT code 88363 for archived tissue retrieval It can't be used to just pull blocks. The slides have to be reviewed and the best block chosen by a pathologist. If there is only one block then the pathologist needs to look at the slides and determine if there is enough tissue for molecular testing. It's a professional charge. Use it on re-accessioned cases for which molecular testing is requested and the best block needs to be chosen. Yes we use it when sending out for Oncotype DX if the case was signed out over 30 days before. Mark On Thu, Jan 10, 2013 at 7:36 AM, Natalie Nagy < Nagy_Natalie@holyokehealth.com> wrote: > Hi everyone, > I just have a question about CPT code 88363, first > can it be used for pulling blocks for Oncotype DX testing, also is > there a time limit on when this code can be used? Does it have to be > within a year, a month, etc...of when the patient account went active? > > Thanks for all the help, > > Natalie J. Nagy (HT)ASCP > Histology Supervisor > Holyoke Medical Center > > > CONFIDENTIALITY NOTICE: This email communication and any attachments > may contain confidential and privileged information for the use of the > designated recipients named above. If you are not the intended > recipient, you are hereby notified that you have received this > communication in error and that any review, disclosure, dissemination, > distribution or copying of it or its contents is prohibited. If you > have received this communication in error, please reply to the sender > immediately and destroy all copies of this communication and any > attachments. For further information regarding Holyoke Medical > Center's privacy policy, Please visit our Internet web site at > http://www.holyokehealth.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Joyce.Weems <@t> emoryhealthcare.org Thu Jan 10 17:02:14 2013 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Thu Jan 10 17:02:49 2013 Subject: [Histonet] Help with CPT code 88363 for archived tissue retrieval In-Reply-To: References: <661949901A768E4F9CC16D8AF8F2838C03974F73@is-e2k3.grhs.net> <50EED456.7400.0077.1@harthosp.org> Message-ID: Same here - and we are being reimbursed. We don't charge much. Medicare new rate is $12.71 - but every billable test helps when that is what our productivity is based on! Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: Victor A. Tobias [mailto:vtobias@uw.edu] Sent: Thursday, January 10, 2013 5:47 PM To: 'Bell, Lynne'; 'Richard Cartun'; Weems, Joyce K.; 'Mark Tarango'; 'Mike Pence'; 'Natalie Nagy' Cc: 'histonet@lists.utsouthwestern.edu' Subject: RE: [Histonet] Help with CPT code 88363 for archived tissue retrieval We have been billing a tech and pro fee since 2011. Whether we are getting reimbursed for both is another question. Victor Victor Tobias HT(ASCP) Clinical Applications Analyst Harborview Medical Center Dept of Pathology Room NJB244 Seattle, WA 98104 vtobias@u.washington.edu 206-744-2735 206-744-8240 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bell, Lynne Sent: Thursday, January 10, 2013 11:50 AM To: 'Richard Cartun'; Joyce K. Weems; Mark Tarango; Mike Pence; Natalie Nagy Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Help with CPT code 88363 for archived tissue retrieval I agree, Dr. Cartun. I believe that the CPT Coding book specifically says that it is only a professional charge. Lynne Bell, HT (ASCP) Histology Team Leader Central Vermont Medical Center 130 Fisher Road Berlin, VT 05641 802-371-4923 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Thursday, January 10, 2013 2:47 PM To: Joyce K. Weems; Mark Tarango; Mike Pence; Natalie Nagy Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Help with CPT code 88363 for archived tissue retrieval I'm not 100% sure, but I don't think this CPT code has a "Technical" component. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-2204 Fax >>> "Mike Pence" 1/10/2013 2:27 PM >>> So are you putting the charge thru twice or is the charge for the 88363 divided out into tech and prof.? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Thursday, January 10, 2013 1:06 PM To: 'Mark Tarango'; Natalie Nagy Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Help with CPT code 88363 for archived tissue retrieval And I should explain the reason I most know this.. our pathologists were denied because the tech charge hadn't been entered yet. So now I make sure the tech charge is entered before sending to the pathologists billing folks. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Tarango Sent: Thursday, January 10, 2013 2:01 PM To: Natalie Nagy Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Help with CPT code 88363 for archived tissue retrieval It can't be used to just pull blocks. The slides have to be reviewed and the best block chosen by a pathologist. If there is only one block then the pathologist needs to look at the slides and determine if there is enough tissue for molecular testing. It's a professional charge. Use it on re-accessioned cases for which molecular testing is requested and the best block needs to be chosen. Yes we use it when sending out for Oncotype DX if the case was signed out over 30 days before. Mark On Thu, Jan 10, 2013 at 7:36 AM, Natalie Nagy < Nagy_Natalie@holyokehealth.com> wrote: > Hi everyone, > I just have a question about CPT code 88363, first > can it be used for pulling blocks for Oncotype DX testing, also is > there a time limit on when this code can be used? Does it have to be > within a year, a month, etc...of when the patient account went active? > > Thanks for all the help, > > Natalie J. Nagy (HT)ASCP > Histology Supervisor > Holyoke Medical Center > > > CONFIDENTIALITY NOTICE: This email communication and any attachments > may contain confidential and privileged information for the use of the > designated recipients named above. If you are not the intended > recipient, you are hereby notified that you have received this > communication in error and that any review, disclosure, dissemination, > distribution or copying of it or its contents is prohibited. If you > have received this communication in error, please reply to the sender > immediately and destroy all copies of this communication and any > attachments. For further information regarding Holyoke Medical > Center's privacy policy, Please visit our Internet web site at > http://www.holyokehealth.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From one_angel_secret <@t> yahoo.com Thu Jan 10 17:07:13 2013 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Thu Jan 10 17:07:29 2013 Subject: [Histonet] interview In-Reply-To: References: Message-ID: <83051F60-338E-47C1-B14E-E65222D76965@yahoo.com> Hope you got some help. If your not done I would find a little task to see what their manual dexterity is. I would also ask questions to see if any of them did some research on histology before coming to the interview that shows interest and that they are proactive. Your limited on personal questions but you can ask where they see them self 2 years down the road if you give them this opportunity . These are just a couple ideas. Hope you get a go getter and not spoon feeder . Good luck ! Sent from my iPhone On Jan 8, 2013, at 1:02 PM, Gale Limron wrote: > Hello, > I just found out today I will be doing 2nd interviews for 3 candidates for a part time Histology position at our hospital on Friday of this week. These candidates are not histotechs but are willing to do online training and take ASCP board exam within 24 months. I would appreciate some help with what questions to ask. I did not attend the 1st interviews but these were done by our lab manager who does not know a lot about what we do I histology............. > Thank you! > > Gale Limron CT,HT (ASCP) > Histology Supervisor > Union Hospital > 659 Boulevard > Dover, Ohio 44622 > 330-343-3311 ext 2562 > > > > This e-mail is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential or otherwise protected from disclosure. Dissemination, distribution or copying of this e-mail or the information herein by anyone other than the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, is prohibited. If you received this message in error, please delete without copying and kindly e-mail a reply to inform us of the mistake in delivery._______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> gmail.com Thu Jan 10 18:31:10 2013 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Thu Jan 10 18:31:15 2013 Subject: [Histonet] Re: Block retention? Message-ID: I think Joyce Weems has hit the nail on the head here! >>I wish CAP would revisit this. With all the molecular testing coming we will lose tissue that may be valuable to a patient's treatment. I'm hanging on for dear life to what we have.<< I think that policies on retaining blocks need to be reviewed on high. I think we'll see an increase in demand for blocks 10 to 20 years old. The issue is non-trivial, since blocks are bulky and require temperature-controlled storage space. There's also the problem of what will happen to blocks when laboratories close. Bob Richmond Samurai Pathologist Maryville TN From lpwenk <@t> sbcglobal.net Thu Jan 10 19:22:19 2013 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Thu Jan 10 19:22:23 2013 Subject: [Histonet] interview In-Reply-To: References: Message-ID: <364EF7D2C30448D1AB6F56701666B875@HP2010> (Quoted material taken from various ASCP BOC (Board of Certification) webpages.) FIRST: make certain they meet the ASCP HT criteria. If it they are truly doing the OJT route: Route 2: At least 60 semester hours (90 quarter hours) of academic credit from a regionally accredited college/university, with a combination of 12 semester hours (18 quarter hours) of biology and chemistry (must include credit hours in both), or an associate degree from a regionally accredited college/university, with a combination of 12 semester hours (18 quarter hours) of biology and chemistry(must include credit hours in both), AND one year full time acceptable experience in a histopathology (clinical, veterinary, industry or research) laboratory in the U.S., Canada or an accredited laboratory* within the last ten years. *Laboratory accredited by a CMS approved accreditation organization (i.e., AABB, CAP, COLA, DNV, The Joint Commission, etc.). NOTE: FOR U.S. CERTIFICATION THE JOINT COMMISSION INTERNATIONAL (JCI) IS NOT ACCEPTABLE. SECOND: you also say they are willing to do an online training. If that is through a NAACLS-accredited HT program, then the ASCP HT criteria is: Route 1: Successful completion of a NAACLS accredited Histotechnician program within the last 5 years prior to the date of application for examination; So, you need to contact each of the NAACLS programs that offer on-line programs (there's 4-6 of them), and find out what their requirements are. High school graduates, some college, so many college credits, what type and number of biology, chemistry and math requirements, etc. Then you need to make it very clear who is paying (them, the lab, some of both) the thousands of dollars of tuition, buying the books, how much time you will give them each week to work on homework projects (collecting tissue, doing stains, you monitoring them taking exams, etc.). I would have them sign a contract about being a trainee and earning less money than the minimum starting wage until they pass the ASCP HT exam, and then they get a raise to the minimum. And in the contract, if your lab it helping to pay for the tuition or book, that they agree to stay at least, say, 2 years after passing the HT exam, or else they have to pay the lab back some of the money the lab spent on training them (prorated, to amount of time they stayed past the time they passed the ASCP exam). THIRD: If this is a true OJT, notice the "one year full time acceptable experience". You say this is a part-time position, so 1 year of part time does not equal one year full time. This following is from the ASCP BOC webpage. Full-time experience is defined as a minimum of thirty-five (35) hours per week. Individuals who have part-time experience may be permitted to utilize prorated part-time experience to meet the work experience requirements. For example, if you are employed 20 hours per week for one year, your experience would be computed as 20 divided by 35 multiplied by 52 weeks, or the equivalent of 29.7 weeks of full time employment. My concern is your requirement of them taking and passing the ASCP HT exam in 2 years. It might take 2 years for them to earn enough working hours to equal 1 year full time experience. I would suggest that you tell them they must take and pass the HT ASCP exam within 1 year of becoming eligible. If they fail, they can take the exam again in the next quarter (4x/year). If they haven't passed it after 4 attempts, odd are they are not going to pass it. Or if they bother trying again every 3 months, well, that says something about their character also. FOURTH: for your interview questions - open questions. No yes/no. - "tell me about a time you . . . " are great questions. Anyone can make up something that sounds good if they are asked "what would you do if . . .". But asking them to talk about a time when they had to handle a situation gives you an insight into what they did, why, and what they learned from it. And you can keep asking Why or What factors contributed or What would you do differently or What did you learn from this or Tell more more about that time, etc. Don't take "oh, that never happened to me". Oh, yes, all of these have. There are no right answers - it's about what THEY did or how THEY handled a situation. And you can tell them this, to reassure them. What you don't tell them are there are some "wrong" answers - at least ones that you don't want, such as someone saying that everyone they have ever worked with is an idiot, or all their bosses have been incompetent. Some good ideas for questions: Tell me about a time at work (or, at school if they are recently out of school without a lot of work experience) that you . . . - were part of a team (role, contribution, etc.) - dealt with conflict with a coworker or supervisor - worked someplace when the team had a problem working together, or getting the work done effectively/efficiently. - dealt with an angry customer - changed procedures to better serve a customer - you went out of your way to show compassion to someone else - of all the places you have worked at, describe the one you stayed at the longest (why? and why left?) - what factors are influencing you to change your career - how would you rate your attendance record compared with others - give examples of why - during slow times at work, what do you do during that time? - during a busy time at work, how do you maintain a high energy level - when you had to work with someone who was very "different" than you. - worst example of discrimination you've seen in a workplace (co-worker or supervisor) - you had working with a diverse work team - had to deal with a difficult change - the supervisor made changes that you didn't agree with - you had to manage several tasks at the same time - how do you stay organized? What did you do in situations when there just wasn't enough time? - when you initiated change at work (what type? how get others on board? biggest challenge?) - when you had to deal with a lot of stress in a situation. Peggy A. Wenk, HTL(ASCP)SLS Program Director Schools of Histotechnology Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: Gale Limron Sent: Tuesday, January 08, 2013 1:02 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] interview Hello, I just found out today I will be doing 2nd interviews for 3 candidates for a part time Histology position at our hospital on Friday of this week. These candidates are not histotechs but are willing to do online training and take ASCP board exam within 24 months. I would appreciate some help with what questions to ask. I did not attend the 1st interviews but these were done by our lab manager who does not know a lot about what we do I histology............. Thank you! Gale Limron CT,HT (ASCP) Histology Supervisor Union Hospital 659 Boulevard Dover, Ohio 44622 330-343-3311 ext 2562 This e-mail is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential or otherwise protected from disclosure. Dissemination, distribution or copying of this e-mail or the information herein by anyone other than the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, is prohibited. If you received this message in error, please delete without copying and kindly e-mail a reply to inform us of the mistake in delivery._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carole.chotard <@t> fr.netgrs.com Fri Jan 11 04:31:48 2013 From: carole.chotard <@t> fr.netgrs.com (carole.chotard@fr.netgrs.com) Date: Fri Jan 11 04:32:15 2013 Subject: [Histonet] tricks about antibody generated in human on human tissue for IHC Message-ID: Dear all, I work in an histopathology department and we intend to do immunohistochemistry with an antibody generated in human on human tissues. So far, we don't have any experience with that. So I was wondering if somebody would have some feedback to prevent strong background generated by this human antibody on human tissue. We are planning in a first place to decrease the concentration of the secondary antibody Donkey@human which is recommended at 1/200 by Jackson, using it at 1/500 for example. But if anybody would have some experience with this kind of experiments, their tricks would be more than welcome. Best Regards, Carole Chotard Carole Chotard, Ph.D. Pharmacology Research Scientist Molecular Pharmacology and Pathophysiology Department IDRS 125 Chemin de Ronde 78290 Croissy-sur-Seine France Phone : +33 1 55 72 29 48 Fax : +33 1 55 72 25 96 carole.chotard@fr.netgrs.com From dmccaig <@t> ckha.on.ca Fri Jan 11 06:34:55 2013 From: dmccaig <@t> ckha.on.ca (Diana McCaig) Date: Fri Jan 11 06:35:12 2013 Subject: [Histonet] Cresyl violet stain for HP Message-ID: Is anyone willing to share their staining protocol for Cresyl Violet stain for HP Thanks Diana McCaig From brett_connolly <@t> merck.com Fri Jan 11 08:21:52 2013 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Fri Jan 11 08:21:59 2013 Subject: [Histonet] tricks about antibody generated in human on human tissue for IHC In-Reply-To: References: Message-ID: Hi Carole, While I have no direct experience with human on human IHC, my first thought would be to biotinylate your primary antibody and then you would omit the anti-human secondary which is the most likely source of your background staining. If you are using fluorescence you could also conjugate your primary with a fluorophore (http://www.invitrogen.com/site/us/en/home/brands/Molecular-Probes/Key-Molecular-Probes-Products/Zenon-Labeling-Technology.html) Brett M. Connolly, Ph.D. Principal Scientist, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of carole.chotard@fr.netgrs.com Sent: Friday, January 11, 2013 5:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] tricks about antibody generated in human on human tissue for IHC Dear all, I work in an histopathology department and we intend to do immunohistochemistry with an antibody generated in human on human tissues. So far, we don't have any experience with that. So I was wondering if somebody would have some feedback to prevent strong background generated by this human antibody on human tissue. We are planning in a first place to decrease the concentration of the secondary antibody Donkey@human which is recommended at 1/200 by Jackson, using it at 1/500 for example. But if anybody would have some experience with this kind of experiments, their tricks would be more than welcome. Best Regards, Carole Chotard Carole Chotard, Ph.D. Pharmacology Research Scientist Molecular Pharmacology and Pathophysiology Department IDRS 125 Chemin de Ronde 78290 Croissy-sur-Seine France Phone : +33 1 55 72 29 48 Fax : +33 1 55 72 25 96 carole.chotard@fr.netgrs.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From rjbuesa <@t> yahoo.com Fri Jan 11 09:12:47 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jan 11 09:12:51 2013 Subject: [Histonet] tricks about antibody generated in human on human tissue for IHC In-Reply-To: References: Message-ID: <1357917167.12512.YahooMailNeo@web163102.mail.bf1.yahoo.com> About 20 years ago in my lab they were developing an "anti-human melanoma" antibody that we used to test on human melanoma (+) cases and we approached more or less in the way you suggest and were able to eliminate background. You just have to start testing, keep good records of what you do and your results so you can twink your procedure. Ren? J. From: "carole.chotard@fr.netgrs.com" To: histonet@lists.utsouthwestern.edu Sent: Friday, January 11, 2013 5:31 AM Subject: [Histonet] tricks about antibody generated in human on human tissue for IHC Dear all, I work in an histopathology department and we intend to do immunohistochemistry with an antibody generated in human on human tissues. So far, we don't have any experience with that. So I was wondering if somebody would have some feedback to prevent strong background generated by this human antibody on human tissue. We are planning in a first place to decrease the concentration of the secondary antibody Donkey@human which is recommended at 1/200 by Jackson, using it at 1/500 for example. But if anybody would have some experience with this kind of experiments, their tricks would be more than welcome. Best Regards, Carole Chotard Carole Chotard, Ph.D. Pharmacology Research Scientist Molecular Pharmacology and Pathophysiology Department IDRS 125 Chemin de Ronde 78290 Croissy-sur-Seine France Phone : +33 1 55 72 29 48 Fax :? ? ? +33 1 55 72 25 96 carole.chotard@fr.netgrs.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TGoins <@t> mt.gov Fri Jan 11 09:42:46 2013 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Fri Jan 11 09:42:59 2013 Subject: [Histonet] interview In-Reply-To: <364EF7D2C30448D1AB6F56701666B875@HP2010> References: <364EF7D2C30448D1AB6F56701666B875@HP2010> Message-ID: After a tour and review of the work-day expectations, the candidate completes a simple math test (basically dilutions and percentage calculations) and then is asked to observe and repeat the processes of sectioning and coverslipping. Look for signs of disinterest (no questions - the wall of silence) and a lack of the manual dexterity required to make a good slide (if you are fearful every time their hands approach the microtome, that's not a good sign - routine can be learned but spatial awareness not so much). My cohort was hired with zero experience and not certified and she's fabulous! Tresa Goins Histopathology Section Supervisor Montana Veterinary Diagnostic Lab -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk Sent: Thursday, January 10, 2013 6:22 PM To: Gale Limron; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] interview (Quoted material taken from various ASCP BOC (Board of Certification) webpages.) FIRST: make certain they meet the ASCP HT criteria. If it they are truly doing the OJT route: Route 2: At least 60 semester hours (90 quarter hours) of academic credit from a regionally accredited college/university, with a combination of 12 semester hours (18 quarter hours) of biology and chemistry (must include credit hours in both), or an associate degree from a regionally accredited college/university, with a combination of 12 semester hours (18 quarter hours) of biology and chemistry(must include credit hours in both), AND one year full time acceptable experience in a histopathology (clinical, veterinary, industry or research) laboratory in the U.S., Canada or an accredited laboratory* within the last ten years. *Laboratory accredited by a CMS approved accreditation organization (i.e., AABB, CAP, COLA, DNV, The Joint Commission, etc.). NOTE: FOR U.S. CERTIFICATION THE JOINT COMMISSION INTERNATIONAL (JCI) IS NOT ACCEPTABLE. SECOND: you also say they are willing to do an online training. If that is through a NAACLS-accredited HT program, then the ASCP HT criteria is: Route 1: Successful completion of a NAACLS accredited Histotechnician program within the last 5 years prior to the date of application for examination; So, you need to contact each of the NAACLS programs that offer on-line programs (there's 4-6 of them), and find out what their requirements are. High school graduates, some college, so many college credits, what type and number of biology, chemistry and math requirements, etc. Then you need to make it very clear who is paying (them, the lab, some of both) the thousands of dollars of tuition, buying the books, how much time you will give them each week to work on homework projects (collecting tissue, doing stains, you monitoring them taking exams, etc.). I would have them sign a contract about being a trainee and earning less money than the minimum starting wage until they pass the ASCP HT exam, and then they get a raise to the minimum. And in the contract, if your lab it helping to pay for the tuition or book, that they agree to stay at least, say, 2 years after passing the HT exam, or else they have to pay the lab back some of the money the lab spent on training them (prorated, to amount of time they stayed past the time they passed the ASCP exam). THIRD: If this is a true OJT, notice the "one year full time acceptable experience". You say this is a part-time position, so 1 year of part time does not equal one year full time. This following is from the ASCP BOC webpage. Full-time experience is defined as a minimum of thirty-five (35) hours per week. Individuals who have part-time experience may be permitted to utilize prorated part-time experience to meet the work experience requirements. For example, if you are employed 20 hours per week for one year, your experience would be computed as 20 divided by 35 multiplied by 52 weeks, or the equivalent of 29.7 weeks of full time employment. My concern is your requirement of them taking and passing the ASCP HT exam in 2 years. It might take 2 years for them to earn enough working hours to equal 1 year full time experience. I would suggest that you tell them they must take and pass the HT ASCP exam within 1 year of becoming eligible. If they fail, they can take the exam again in the next quarter (4x/year). If they haven't passed it after 4 attempts, odd are they are not going to pass it. Or if they bother trying again every 3 months, well, that says something about their character also. FOURTH: for your interview questions - open questions. No yes/no. - "tell me about a time you . . . " are great questions. Anyone can make up something that sounds good if they are asked "what would you do if . . .". But asking them to talk about a time when they had to handle a situation gives you an insight into what they did, why, and what they learned from it. And you can keep asking Why or What factors contributed or What would you do differently or What did you learn from this or Tell more more about that time, etc. Don't take "oh, that never happened to me". Oh, yes, all of these have. There are no right answers - it's about what THEY did or how THEY handled a situation. And you can tell them this, to reassure them. What you don't tell them are there are some "wrong" answers - at least ones that you don't want, such as someone saying that everyone they have ever worked with is an idiot, or all their bosses have been incompetent. Some good ideas for questions: Tell me about a time at work (or, at school if they are recently out of school without a lot of work experience) that you . . . - were part of a team (role, contribution, etc.) - dealt with conflict with a coworker or supervisor - worked someplace when the team had a problem working together, or getting the work done effectively/efficiently. - dealt with an angry customer - changed procedures to better serve a customer - you went out of your way to show compassion to someone else - of all the places you have worked at, describe the one you stayed at the longest (why? and why left?) - what factors are influencing you to change your career - how would you rate your attendance record compared with others - give examples of why - during slow times at work, what do you do during that time? - during a busy time at work, how do you maintain a high energy level - when you had to work with someone who was very "different" than you. - worst example of discrimination you've seen in a workplace (co-worker or supervisor) - you had working with a diverse work team - had to deal with a difficult change - the supervisor made changes that you didn't agree with - you had to manage several tasks at the same time - how do you stay organized? What did you do in situations when there just wasn't enough time? - when you initiated change at work (what type? how get others on board? biggest challenge?) - when you had to deal with a lot of stress in a situation. Peggy A. Wenk, HTL(ASCP)SLS Program Director Schools of Histotechnology Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: Gale Limron Sent: Tuesday, January 08, 2013 1:02 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] interview Hello, I just found out today I will be doing 2nd interviews for 3 candidates for a part time Histology position at our hospital on Friday of this week. These candidates are not histotechs but are willing to do online training and take ASCP board exam within 24 months. I would appreciate some help with what questions to ask. I did not attend the 1st interviews but these were done by our lab manager who does not know a lot about what we do I histology............. Thank you! Gale Limron CT,HT (ASCP) Histology Supervisor Union Hospital 659 Boulevard Dover, Ohio 44622 330-343-3311 ext 2562 This e-mail is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential or otherwise protected from disclosure. Dissemination, distribution or copying of this e-mail or the information herein by anyone other than the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, is prohibited. If you received this message in error, please delete without copying and kindly e-mail a reply to inform us of the mistake in delivery._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From robert_schoonhoven <@t> yahoo.com Fri Jan 11 10:05:45 2013 From: robert_schoonhoven <@t> yahoo.com (Robert Schoonhoven) Date: Fri Jan 11 10:05:49 2013 Subject: [Histonet] Looking for Histology work in the Newark DE area Message-ID: <1357920345.8384.YahooMailNeo@web126206.mail.ne1.yahoo.com> I am a Certified Histotechnologist looking for temp or perm assignment within 30 miles of Newark DE. I have over 25 years experience in all aspects of histoloy, immunohistochemistry, image analysis and microscopy. ? If interested please reply direct to the following email address: 1schoonhoven1@gmail.com ? Robert Schoonhoven, HT/HTL (ASCP) From Timothy.Morken <@t> ucsfmedctr.org Fri Jan 11 10:31:57 2013 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Fri Jan 11 10:32:20 2013 Subject: [Histonet] tricks about antibody generated in human on human tissue for IHC In-Reply-To: References: Message-ID: <761E2B5697F795489C8710BCC72141FF05171E@ex07.net.ucsf.edu> Carole, Look at Golden Bridge International Labs. They have a human-on-human detection system. http://gbi-inc.com/ I've worked with them before and they have excellent products. They also supply some products to other vendors that are rebadged. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of carole.chotard@fr.netgrs.com Sent: Friday, January 11, 2013 2:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] tricks about antibody generated in human on human tissue for IHC Dear all, I work in an histopathology department and we intend to do immunohistochemistry with an antibody generated in human on human tissues. So far, we don't have any experience with that. So I was wondering if somebody would have some feedback to prevent strong background generated by this human antibody on human tissue. We are planning in a first place to decrease the concentration of the secondary antibody Donkey@human which is recommended at 1/200 by Jackson, using it at 1/500 for example. But if anybody would have some experience with this kind of experiments, their tricks would be more than welcome. Best Regards, Carole Chotard Carole Chotard, Ph.D. Pharmacology Research Scientist Molecular Pharmacology and Pathophysiology Department IDRS 125 Chemin de Ronde 78290 Croissy-sur-Seine France Phone : +33 1 55 72 29 48 Fax : +33 1 55 72 25 96 carole.chotard@fr.netgrs.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histotech <@t> imagesbyhopper.com Fri Jan 11 10:43:46 2013 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Fri Jan 11 10:44:04 2013 Subject: [Histonet] Medical Director credentials on reports In-Reply-To: <89F4666A496DC949A819ECC40E11C867BF56FABA@EXCHANGEMB1.hmc.hurleymc.com> References: <001501cdef38$d2e80340$78b809c0$@imagesbyhopper.com>, <453E2357-6988-4A70-8B48-B21113554C5C@yahoo.com> <89F4666A496DC949A819ECC40E11C867BF56FABA@EXCHANGEMB1.hmc.hurleymc.com> Message-ID: <003601cdf01a$d95b2ee0$8c118ca0$@imagesbyhopper.com> Bringing this up again, as it would be nice to have a definitive answer about this. Will, do you have a reg number we can work with? Thanks for the assistance! Michelle -----Original Message----- From: Lynette Pavelich [mailto:LPaveli1@hurleymc.com] Sent: Thursday, January 10, 2013 9:45 AM To: Will Chappell; Cc: Histonet@Lists. Utsouthwestern. Edu Subject: RE: [Histonet] Medical Director credentials on reports Searched for the CLIA regulation pertaining to this but unable to locate. Do you have the regulation code # readily available? Lynette ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Will Chappell [chapcl@yahoo.com] Sent: Thursday, January 10, 2013 8:58 AM To: Cc: Histonet@Lists. Utsouthwestern. Edu Subject: Re: [Histonet] Medical Director credentials on reports Just passed CLIA. It is a CLIA requirement. Not sure about CAP. Will Chappell CHOC Children's Sent from my iPhone On Jan 10, 2013, at 5:45 AM, wrote: > Does anyone know, and maybe have the regs, whether either the Joint > Commission or the CAP *require* that the Medical Director's > credentials be included on the genlab and surgical reports? I have > heard that it *is* a requirement and now am hearing that it is *not* > one. Clarification would be nice! > > Example: > > Joe Smith > Medical Director > > OR > > Joe Smith, M.D. > Medical Director > > Thanks! > Michelle > ----- > No virus found in this message. > Checked by AVG - www.avg.com > Version: 2013.0.2805 / Virus Database: 2637/6021 - Release Date: > 01/09/13 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----- No virus found in this message. Checked by AVG - www.avg.com Version: 2013.0.2805 / Virus Database: 2637/6021 - Release Date: 01/09/13 ----- No virus found in this message. Checked by AVG - www.avg.com Version: 2013.0.2805 / Virus Database: 2637/6023 - Release Date: 01/10/13 From BMolinari <@t> texasheart.org Fri Jan 11 11:31:02 2013 From: BMolinari <@t> texasheart.org (Molinari, Betsy) Date: Fri Jan 11 11:31:48 2013 Subject: [Histonet] "harder" paraffin Message-ID: Hi Histonetters, Good Friday to you all. Do you know if there is a ?harder? paraffin that may offer more support to the tissue while cutting? I currently use Paraplast Plus and love it, but now I am cutting some polymer material and there is some tearing and was thinking that maybe a different paraffin would help. I am trying to avoid embedding in plastic. Thanks, Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 1101 Bates Street Houston,TX 77030 832-355-6524 (lab) 832-355-6812 (fax) [THI Celebrates 50 Years] Betsy Molinari Senior Histology Research Technician 832-355-6524 | BMolinari@texasheart.org www.texasheart.org TEXAS HEART INSTITUTE 6770 Bertner Ave. MC 1-283 | Houston, TX 77030 [Receive electronic news from THI][THI on Facebook][THI on Twitter][THI on YouTube][THI's Flickr page] CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient, or an authorized representative of the intended recipient, you are hereby notified that any review or dissemination or copying of this e-mail and its attachments, if any, or the information contained herein, is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. From ratliffjack <@t> hotmail.com Fri Jan 11 11:45:53 2013 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Fri Jan 11 11:46:01 2013 Subject: [Histonet] "harder" paraffin In-Reply-To: References: Message-ID: Hello Betsy! I use Leica's EM400 for my paraffin embedding needs and it does extremely well for all of my decalcified bone work. Of course I mostly work with undemineralized bone, biomaterials and medical device implants so I pretty routinely utilize acrylic resins (MMA) for most of my work. With that said, if you need any help or assistance should you need to go the route of plastic embedding, feel free to contact me. One quick note, I also have access to a non-contact laser (laser microtome) that can cut thin micron linear or 3D sections of fresh or resin embedded tissues. If you are interested, I would love to see if this technology could help service your needs if you have some spare tissue samples that you could send over to me. Best Regards, Jack Jack L. RatliffOwner/Histologist - Ratliff Histology Consultants, LLCChairman, Hard Tissue Committee - National Society for Histotechnology317-281-1975jratliff@ratliffhistology.com From: BMolinari@texasheart.org To: Histonet@lists.utsouthwestern.edu Date: Fri, 11 Jan 2013 17:31:02 +0000 CC: Subject: [Histonet] "harder" paraffin Hi Histonetters, Good Friday to you all. Do you know if there is a ?harder? paraffin that may offer more support to the tissue while cutting? I currently use Paraplast Plus and love it, but now I am cutting some polymer material and there is some tearing and was thinking that maybe a different paraffin would help. I am trying to avoid embedding in plastic. Thanks, Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 1101 Bates Street Houston,TX 77030 832-355-6524 (lab) 832-355-6812 (fax) [THI Celebrates 50 Years] Betsy Molinari Senior Histology Research Technician 832-355-6524 | BMolinari@texasheart.org www.texasheart.org TEXAS HEART INSTITUTE 6770 Bertner Ave. MC 1-283 | Houston, TX 77030 [Receive electronic news from THI][THI on Facebook][THI on Twitter][THI on YouTube][THI's Flickr page] CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient, or an authorized representative of the intended recipient, you are hereby notified that any review or dissemination or copying of this e-mail and its attachments, if any, or the information contained herein, is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ppinchr <@t> yahoo.com Fri Jan 11 11:51:01 2013 From: ppinchr <@t> yahoo.com (RP) Date: Fri Jan 11 11:51:06 2013 Subject: [Histonet] Full Time Contract Position Message-ID: <1357926661.61185.YahooMailNeo@web162006.mail.bf1.yahoo.com> I'm in need of a Certified Histology Tech with grossing experience in the Houston, Texas area. Please contact me privately for more information. ? Thanks, Rachel From dmccaig <@t> ckha.on.ca Fri Jan 11 12:25:54 2013 From: dmccaig <@t> ckha.on.ca (Diana McCaig) Date: Fri Jan 11 12:26:22 2013 Subject: [Histonet] LEICA 6025 PROCESSOR Message-ID: We are going to be setting up a Leica 6025 Processor. Can anyone send me their SOP and worksheets that you have created so I can use them as a reference and modify them according to our usage. Would truly be appreciated as well as any helpful tips or tricks you have encountered. Diana From carl.hobbs <@t> kcl.ac.uk Fri Jan 11 12:32:36 2013 From: carl.hobbs <@t> kcl.ac.uk (Hobbs, Carl) Date: Fri Jan 11 12:32:47 2013 Subject: [Histonet] Re: zebra fish experts...... Message-ID: <00D6B8253EAED840B8D04E23549738181981EC38@AM2PRD0311MB399.eurprd03.prod.outlook.com> Why are you fixing ? Try unfixed frozen? Or, why not Pwax embedded? They cut well in Pwax. Sure, you may have to "polish" the cut surface with tissue damped in water/20% alcohol to prevent cracking/curling just before each section is cut. Or, after fixation, place in 30% sucrose until kidneys sink. Store at 4C until you freeze ( if not immediately). You will have to try several approaches until you find the correct one for your Lab. Good luck. Carl Hobbs Histology and Imaging Manager Wolfson CARD School of Biomedical Sciences Kings College London Guys Campus SE1 1UL Tel: 020 78486813 Fax: 020 78486816 020 78486813 From rsrichmond <@t> gmail.com Fri Jan 11 12:53:11 2013 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Fri Jan 11 12:53:17 2013 Subject: [Histonet] Re: Cresyl echt violet stain for Helicobacter Message-ID: Diana McCaig asked if anyone had a method for cresyl echt violet staining of Helicobacter. This information was published in Histo-Logic in April 1999. This was all the information in the article. Cresyl Echt Violet (CEV) This method utilizes a metachromatic dye, cresyl echt violet (also called cresyl violet acetate) to stain the bacteria. The bacteria stand out well because the mucous layer does not stain heavily with the CEV and provides a nice contrast for the organism. It is a very easy and quick stain utilizing a 0.1% aqueous working solution of CEV. The slides are deparaffinized, hydrated to water, and stained in the CEV for 3 minutes. They are then rinsed in distilled water, dehydrated, cleared, and mounted. Reference: Gomes C. Rapid cresyl echt violet staining method for identifying Helicobacter pylori. On Stage, NY State Histotechnological Society. 1993;16(2). I recently had a locum tenens client who used this stain. I was not impressed that it was significantly better than the blue stain. They also had the immunostain available. I can probably get more information on the method from them. Bob Richmond Samurai Pathologist Maryville TN From madeleinehuey <@t> gmail.com Fri Jan 11 13:25:35 2013 From: madeleinehuey <@t> gmail.com (Madeleine Huey) Date: Fri Jan 11 13:25:40 2013 Subject: [Histonet] Re: Histonet Digest, Vol 110, Issue 16 In-Reply-To: <50f05333.6aaeb60a.09b2.ffff8ccfSMTPIN_ADDED_MISSING@mx.google.com> References: <50f05333.6aaeb60a.09b2.ffff8ccfSMTPIN_ADDED_MISSING@mx.google.com> Message-ID: Betsy, Try the Paraplast Plus X-TRA. You can mix X-TRA with Plus (3:1) ratio is too hard for your blade. Good Luck! madeleine_h@elcaminohospital.org From: BMolinari@texasheart.org To: Histonet@lists.utsouthwestern.edu Date: Fri, 11 Jan 2013 17:31:02 +0000 CC: Subject: [Histonet] "harder" paraffin Hi Histonetters, Good Friday to you all. Do you know if there is a ?harder? paraffin that may offer more support to the tissue while cutting? I currently use Paraplast Plus and love it, but now I am cutting some polymer material and there is some tearing and was thinking that maybe a different paraffin would help. I am trying to avoid embedding in plastic. Thanks, Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 1101 Bates Street Houston,TX 77030 832-355-6524 (lab) 832-355-6812 (fax) On Fri, Jan 11, 2013 at 10:00 AM, wrote: > From: BMolinari@texasheart.org > To: Histonet@lists.utsouthwestern.edu > Date: Fri, 11 Jan 2013 17:31:02 +0000 > CC: > Subject: [Histonet] "harder" paraffin > > Hi Histonetters, > Good Friday to you all. Do you know if there is a ?harder? paraffin that may offer more support to the tissue while cutting? I currently use Paraplast Plus and love it, but now I am cutting some polymer material and there is some tearing and was thinking that maybe a different paraffin would help. I am trying to avoid embedding in plastic. > Thanks, > Betsy Molinari HT (ASCP) > Texas Heart Institute > Cardiovascular Pathology > 1101 Bates Street > Houston,TX 77030 > 832-355-6524 (lab) > 832-355-6812 (fax) > > From madeleinehuey <@t> gmail.com Fri Jan 11 13:56:53 2013 From: madeleinehuey <@t> gmail.com (Madeleine Huey) Date: Fri Jan 11 13:56:57 2013 Subject: [Histonet] Re: Histonet Digest, Vol 110, Issue 15 In-Reply-To: <50f04cad.23dcb60a.2a0c.ffff9032SMTPIN_ADDED_MISSING@mx.google.com> References: <50f04cad.23dcb60a.2a0c.ffff9032SMTPIN_ADDED_MISSING@mx.google.com> Message-ID: Colleen, You can pre-warm your OCT block inside the cryostat @ -20c (~ hr) from freezer before cutting, then gradually increase the temperature to warmer with 1 degree increment (thickness dependant). I also pre-chilled my slides before used. Another alternative; - Fix with 4% PFA/PBS for ~ 1 hr on ice (you can freezed aliquot 4%PFA @ -20c so you don't need to make them fresh every time) - Wash 3xPBS buffer to remove excess 4% PFA/PBS, ph 7.4 - Transfer pre-fix zebra fish to 15% & 30% sucrose (30 min each or longer, not crucial), or you can leave them in 30% sucrose & store in fridge without freezing in OCT - Embed with OCT on ICE, not liquid nitrogen - Pre-warm -20c before cutting Good Luck! Madeleine Huey, BS, HTL & QIHC (ASCP) Supervisor - Pathology (IPOX & Histology) madeleine_h@elcaminohospital.org Message: 1 Date: Thu, 10 Jan 2013 15:16:32 -0600 From: Colleen Forster Subject: [Histonet] zebra fish experts...... To: Histonet Message-ID: <50EF2FB0.6030908@umn.edu> Content-Type: text/plain; charset="iso-8859-1" Anyone out there doing zebra fish work I need your help! We are trying to fix zebra fish kidneys in 10% formalin and then freeze...the samples just seem to crumble when we try to cut...can someone help me out here with fixing/freezing methods for these little buggers!! Thanks in advance!! Colleen Forster U of MN From carl.hobbs <@t> kcl.ac.uk Fri Jan 11 14:00:18 2013 From: carl.hobbs <@t> kcl.ac.uk (Hobbs, Carl) Date: Fri Jan 11 14:00:30 2013 Subject: [Histonet] Re: tricks about antibody generated in human on human Message-ID: <00D6B8253EAED840B8D04E235497381819822DE7@AMSPRD0311MB400.eurprd03.prod.outlook.com> Good advice given. Biotinylated primaries to be followed by stAvidin-488 or stABCpx-DAB, for eg. Or, if biotinylated primary gives good result with above but, at low dilution factor, use tyramide amplification? Human- on- human can be just fine, depending on what proteins you want to demonstrate and, in what tissue. What tissue are you working on? What tissue prep ( Pwax/frozen)? You using patients' serum containing auto-antibodies? Or, do you have a human that you are injecting human Ags into, then bleeding that person so you can harvest Abs? Scary! NB: You suggested diluting out your secondary...not good as you will just lower your specific signal as well, imho. Curious Carl Carl Hobbs Histology Manager Wolfson CARD School of Biomedical Sciences Kings College London Guys Campus SE1 1UL Tel: 020 78486813 Fax: 020 78486816 020 78486813 From rjbuesa <@t> yahoo.com Fri Jan 11 15:18:15 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jan 11 15:18:20 2013 Subject: [Histonet] "harder" paraffin In-Reply-To: References: Message-ID: <1357939095.37483.YahooMailNeo@web163101.mail.bf1.yahoo.com> Merk (Darmstad, Germany) used to manufacture paraffin waxes of 63?C and 65?C, hard enough to be used in plant microtomy. They are all whitish. Try contacting Merk, or perhaps Adrich. Ren? J. From: "Molinari, Betsy" To: "Histonet@lists.utsouthwestern.edu" Sent: Friday, January 11, 2013 12:31 PM Subject: [Histonet] "harder" paraffin Hi Histonetters, Good Friday to you all. Do you know if there is a ?harder? paraffin? that may offer more support to the tissue while cutting? I currently use Paraplast Plus and love it, but now I am cutting some polymer material and there is some tearing and was thinking that maybe a different paraffin would help. I am trying to avoid embedding in plastic. Thanks, Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 1101 Bates Street Houston,TX 77030 832-355-6524 (lab) 832-355-6812 (fax) [THI Celebrates 50 Years] Betsy Molinari Senior Histology Research Technician 832-355-6524 | BMolinari@texasheart.org www.texasheart.org TEXAS HEART INSTITUTE 6770 Bertner Ave. MC 1-283 | Houston, TX 77030 [Receive electronic news from THI][THI on Facebook][THI on Twitter][THI on YouTube][THI's Flickr page] CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient, or an authorized representative of the intended recipient, you are hereby notified that any review or dissemination or copying of this e-mail and its attachments, if any, or the information contained herein, is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Sat Jan 12 02:00:39 2013 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sat Jan 12 02:06:07 2013 Subject: [Histonet] literature on molecular diagnostic Message-ID: <002601cdf09a$ea696d20$bf3c4760$@gmx.at> Dear histonetters! We are looking for good books on molecular pathology and molecular diagnostics for our pathologists. Also some technical background is appreciated. And it should be up to date. Can anyone recommend a good book? Many thanks in advance Gudrun Lang From junjieliang1987 <@t> hotmail.com Sat Jan 12 18:33:08 2013 From: junjieliang1987 <@t> hotmail.com (Jun Jie Liang) Date: Sat Jan 12 18:33:19 2013 Subject: [Histonet] RE:literature on molecular diagnostic (Gudrun Lang) In-Reply-To: References: Message-ID: Hi Lang:There are two books I would recommend to you. 1. Molecular pathology-The molecular basis of human disease, edited by William B.Coleman and Gregory J. Tsongalis2. Molecular Diagnostics: Fundamentals,Metheods &Clinical Applications by Lela Buckingham (Highly recommended!) There is also a online molecular pathology program from American Association for Clinical Chemistry(http://aacc.org/development/certificates/molpathcertprog/pages/default.aspx)I hope these will help. Regards,Jun Jie Liang, HT(ASCP)> > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > Message: 8 > Date: Sat, 12 Jan 2013 09:00:39 +0100 > From: "Gudrun Lang" > Subject: [Histonet] literature on molecular diagnostic > To: > Message-ID: <002601cdf09a$ea696d20$bf3c4760$@gmx.at> > Content-Type: text/plain; charset="us-ascii" > > Dear histonetters! > We are looking for good books on molecular pathology and molecular > diagnostics for our pathologists. > Also some technical background is appreciated. And it should be up to date. > > Can anyone recommend a good book? > > Many thanks in advance > Gudrun Lang > > > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 110, Issue 17 > ***************************************** From Gary_Steinke <@t> vwr.com Sun Jan 13 12:05:40 2013 From: Gary_Steinke <@t> vwr.com (Gary_Steinke@vwr.com) Date: Sun Jan 13 12:05:46 2013 Subject: [Histonet] Gary Steinke is out of the office Message-ID: I will be out of the office starting 01/13/2013 and will not return until 01/17/2013. I will be away on Business and will have no computer access unitl 1-17 as it's being updated. If you need immediate help, please contact our Healthcare Customer Care Group at 877-881-1192 or by email at HEALTHCARESERVICE@VWR.COM. Thank you. From ttroyer <@t> petersonlab.com Mon Jan 14 14:10:39 2013 From: ttroyer <@t> petersonlab.com (Travis Troyer) Date: Mon Jan 14 14:10:48 2013 Subject: [Histonet] Retrieving section off of an H&E stained slide for immunos Message-ID: <91AC3B15DD3448E987F93339AB90ED63@Peterson.local> I was wondering if anyone has heard of any method of removing H&E stained sections from a slide and placing them on a charged slide for immunostaining. Thanks Travis From DSiena <@t> statlab.com Mon Jan 14 14:22:00 2013 From: DSiena <@t> statlab.com (Debra Siena) Date: Mon Jan 14 14:22:04 2013 Subject: [Histonet] Retrieving section off of an H&E stained slide for immunos In-Reply-To: <91AC3B15DD3448E987F93339AB90ED63@Peterson.local> References: <91AC3B15DD3448E987F93339AB90ED63@Peterson.local> Message-ID: Yes, it is called Mount Quick and Newcomer Supply sells it Debbie Siena -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Travis Troyer Sent: Monday, January 14, 2013 2:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Retrieving section off of an H&E stained slide for immunos I was wondering if anyone has heard of any method of removing H&E stained sections from a slide and placing them on a charged slide for immunostaining. Thanks Travis _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lcolbert <@t> pathmdlabs.com Mon Jan 14 15:01:39 2013 From: lcolbert <@t> pathmdlabs.com (Laurie Colbert) Date: Mon Jan 14 15:06:48 2013 Subject: [Histonet] Histotech Position for Private Lab in West Hollywood, CA Message-ID: <12ECD7346266D74691EC2BFC75285E4501197457@BFL323E10.pathmdlabs.local> PATH MD, a private lab in West Hollywood, CA, is looking for an experienced histotech to work per diem starting immediately. There is a strong possibility that this position will turn into a full time position soon. The hours are 11:00 pm to approximately 4:00 am, Monday-Friday. This candidate needs to have strong troubleshooting skills and have the ability to work on their own. The duties include all aspects of routine histology - embedding, cutting, performing manual special stains, correlating slides and reports, and equipment PM/maintenance. ASCP certification strongly preferred. Please submit resumes to: lcolbert@pathmdlabs.com Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab From baazaouinarjes <@t> gmail.com Tue Jan 8 20:20:31 2013 From: baazaouinarjes <@t> gmail.com (Narjes Baazaoui) Date: Mon Jan 14 17:04:16 2013 Subject: [Histonet] fixation, tissue processing for paraffin embedding and sectiong of the mouse eye Message-ID: <2DCEE780-CB04-4006-A87E-E42BEAC9725A@gmail.com> Hello I need the protocol for fixation, tissue processing and mouse eye sectioning if you have it can you please send it to me. thank you Narjes Baazaoui From estellamireles <@t> gmail.com Tue Jan 15 08:09:49 2013 From: estellamireles <@t> gmail.com (Stella Mireles) Date: Tue Jan 15 08:09:53 2013 Subject: [Histonet] Independent Equipment Service Companies Message-ID: I lost the contact number I had for a company that will service histology equipment. Could you please give me some contact info. Thank You Stella From trathborne <@t> somerset-healthcare.com Tue Jan 15 08:45:40 2013 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Tue Jan 15 08:45:58 2013 Subject: [Histonet] Independent Equipment Service Companies In-Reply-To: References: Message-ID: <3AD061FE740D464FAC7BF6B5CFB757071F20375A@smcmail02.somerset-healthcare.com> Where are you located? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stella Mireles Sent: Tuesday, January 15, 2013 9:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Independent Equipment Service Companies I lost the contact number I had for a company that will service histology equipment. Could you please give me some contact info. Thank You Stella _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Caroline.Pratt <@t> uphs.upenn.edu Tue Jan 15 09:52:18 2013 From: Caroline.Pratt <@t> uphs.upenn.edu (Pratt, Caroline) Date: Tue Jan 15 09:52:32 2013 Subject: [Histonet] Interstate Pathology and Consultations Message-ID: I know this is a relatively hot topic and there are varying interpretations of the law on the issue of Interstate Pathology and Consultations. I have read CAP's stance, but I was wondering if anyone has any good articles, grids, charts, tables, etc. on interstate pathology practices. I am particularly looking for the states that do not allow even consultations by out of state pathologists and details on guidelines for interstate consultation for the more stringent states. Thanks for any guidance you can provide. Car J Caroline M. Pratt, MBA Practice Administrator Dermpath 3020 Market Street, Ste 201 Philadelphia, PA 19104 Phone 215-349-8178 Fax 215-662-6150 The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From pruegg <@t> ihctech.net Tue Jan 15 11:06:14 2013 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Tue Jan 15 11:06:17 2013 Subject: [Histonet] FW: [IHCRG] ms abs on rabbit tissue Message-ID: <8ECC102551684E94B2F93DB0ADC5FE39@DESKTOP3> _____ From: ihcrg@googlegroups.com [mailto:ihcrg@googlegroups.com] On Behalf Of Patsy Ruegg Sent: Tuesday, January 15, 2013 9:49 AM To: ihcrg@googlegroups.com Subject: [IHCRG] ms abs on rabbit tissue Does anyone have a mouse ab worked out on rabbit tissue? We got a ms ab from Abcam that is supposed react in Human, rat and rabbit but we are having trouble getting it to stain the rabbit tissue. Please advise. Patsy Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting, LLC 40864 Arkansas Ave Bennett, CO 80102 Fax: 720-859-4110 pruegg@ihctech.net -- You received this message because you are subscribed to the Google Groups "ihcrg" group. The IHC Resource Group is a standing committee within the National Society for Histotechnology. To post to this group, send email to ihcrg@googlegroups.com To unsubscribe from this group, send email to ihcrg+unsubscribe@googlegroups.com For more options, visit this group at http://groups.google.com/group/ihcrg?hl=en To contact the National Society for Histotechnology, email: histo@nsh.org or call 443.535.4060. From donna_suresch <@t> merck.com Tue Jan 15 12:07:44 2013 From: donna_suresch <@t> merck.com (Suresch, Donna L.) Date: Tue Jan 15 12:07:54 2013 Subject: [Histonet] Permanent Fluorescent Mounting Media Message-ID: <2C9A1D9608959940943F357E0A470FF8B00A370CFF@USCTMXP51005.merck.com> Hello Histonet, Anyone know of a non-aqueous permanent mounting media for use with fluorescent IHC? Donna L. Suresch Senior Imaging Research Scientist Merck Research Laboratories Department of Imaging - West Point Campus Mail Stop: WP44K Office: WP44-H129 770 Sumneytown Pike PO Box 4 West Point, PA 19486-0004 Phone: 215-652-7349 Fax: 215-993-6803 Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From JWatson <@t> gnf.org Tue Jan 15 12:25:35 2013 From: JWatson <@t> gnf.org (James Watson) Date: Tue Jan 15 12:25:40 2013 Subject: [Histonet] RE: Permanent Fluorescent Mounting Media In-Reply-To: <2C9A1D9608959940943F357E0A470FF8B00A370CFF@USCTMXP51005.merck.com> References: <2C9A1D9608959940943F357E0A470FF8B00A370CFF@USCTMXP51005.merck.com> Message-ID: Has anyone used hardmount mounting medias for fluorescence in a high volume situation (up to 120 slides a day at times) when the slides are scanned? We have tested BioCares, Thermofishers, and Vectashields. We found that we liked the Thermo Immu-Mount best. Biocare Fluoro care antifade mountant worked, but with scanning we saw some stains were out of focus. The VECTASHIELD HardSet Mounting Medium with DAPI gave us a higher percentage of fading and out of focus images. Our issue with the hardmount and scanning is that we see one edge of the slide in focus and the other out of focus frequently. We are working on our mounting technique to see if this can be corrected by modifying our coverslipping technique. Any suggestions would be appreciated. This does not make your fluorescence permanent since most fluorochromes fade with age. James Watson HT? ASCP GNF? Genomics Institute of the Novartis Research Foundation Tel??? 858-332-4647 Fax?? 858-812-1915 jwatson@gnf.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Suresch, Donna L. Sent: Tuesday, January 15, 2013 10:08 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Permanent Fluorescent Mounting Media Hello Histonet, Anyone know of a non-aqueous permanent mounting media for use with fluorescent IHC? Donna L. Suresch Senior Imaging Research Scientist Merck Research Laboratories Department of Imaging - West Point Campus Mail Stop: WP44K Office: WP44-H129 770 Sumneytown Pike PO Box 4 West Point, PA 19486-0004 Phone: 215-652-7349 Fax: 215-993-6803 Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Elizabeth.Cameron <@t> jax.org Tue Jan 15 12:27:37 2013 From: Elizabeth.Cameron <@t> jax.org (Elizabeth Cameron) Date: Tue Jan 15 12:27:43 2013 Subject: [Histonet] histosearch Message-ID: Anyone else having issues with histosearch today???? The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as possible. From patpxs <@t> gmail.com Tue Jan 15 12:49:43 2013 From: patpxs <@t> gmail.com (Paula Sicurello) Date: Tue Jan 15 12:49:51 2013 Subject: [Histonet] RE: Permanent Fluorescent Mounting Media In-Reply-To: References: <2C9A1D9608959940943F357E0A470FF8B00A370CFF@USCTMXP51005.merck.com> Message-ID: James, When I was at MCV we used Crystal Mount for our frozen IF's (skin, kidney, etc.) You put a few drops on, place it in the oven for a little bit (I do not recall the temperture of the oven) and you had a nice hard cover over your sections. I hope this helps. Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 On Tue, Jan 15, 2013 at 1:25 PM, James Watson wrote: > Has anyone used hardmount mounting medias for fluorescence in a high volume situation (up to 120 slides a day at times) when the slides are scanned? We have tested BioCares, Thermofishers, and Vectashields. We found that we liked the Thermo Immu-Mount best. Biocare Fluoro care antifade mountant worked, but with scanning we saw some stains were out of focus. The VECTASHIELD HardSet Mounting Medium with DAPI gave us a higher percentage of fading and out of focus images. > > Our issue with the hardmount and scanning is that we see one edge of the slide in focus and the other out of focus frequently. We are working on our mounting technique to see if this can be corrected by modifying our coverslipping technique. > > Any suggestions would be appreciated. This does not make your fluorescence permanent since most fluorochromes fade with age. > > James Watson HT ASCP > GNF Genomics Institute of the Novartis Research Foundation > Tel 858-332-4647 > Fax 858-812-1915 > jwatson@gnf.org > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Suresch, Donna L. > Sent: Tuesday, January 15, 2013 10:08 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Permanent Fluorescent Mounting Media > > Hello Histonet, > Anyone know of a non-aqueous permanent mounting media for use with fluorescent IHC? > > Donna L. Suresch > Senior Imaging Research Scientist > Merck Research Laboratories > Department of Imaging - West Point Campus > Mail Stop: WP44K Office: WP44-H129 > 770 Sumneytown Pike > PO Box 4 > West Point, PA 19486-0004 > Phone: 215-652-7349 > Fax: 215-993-6803 > > > Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at > http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tina.vanmeter <@t> gmail.com Tue Jan 15 12:51:23 2013 From: tina.vanmeter <@t> gmail.com (Tina Van Meter) Date: Tue Jan 15 12:51:37 2013 Subject: [Histonet] Permanent Fluorescent Mounting Media In-Reply-To: <2C9A1D9608959940943F357E0A470FF8B00A370CFF@USCTMXP51005.merck.com> References: <2C9A1D9608959940943F357E0A470FF8B00A370CFF@USCTMXP51005.merck.com> Message-ID: <26143202-3509-4A58-A223-F13DAEEE8CEC@gmail.com> ProLong Gold (Invitrogen) Sent from my iPhone On Jan 15, 2013, at 1:07 PM, "Suresch, Donna L." wrote: > Hello Histonet, > Anyone know of a non-aqueous permanent mounting media for use with fluorescent IHC? > > Donna L. Suresch > Senior Imaging Research Scientist > Merck Research Laboratories > Department of Imaging - West Point Campus > Mail Stop: WP44K Office: WP44-H129 > 770 Sumneytown Pike > PO Box 4 > West Point, PA 19486-0004 > Phone: 215-652-7349 > Fax: 215-993-6803 > > > Notice: This e-mail message, together with any attachments, contains > information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, > New Jersey, USA 08889), and/or its affiliates Direct contact information > for affiliates is available at > http://www.merck.com/contact/contacts.html) that may be confidential, > proprietary copyrighted and/or legally privileged. It is intended solely > for the use of the individual or entity named on this message. If you are > not the intended recipient, and have received this message in error, > please notify us immediately by reply e-mail and then delete it from > your system. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lldewe <@t> gmail.com Tue Jan 15 13:05:07 2013 From: lldewe <@t> gmail.com (Loralei Dewe) Date: Tue Jan 15 13:05:10 2013 Subject: [Histonet] Permanent Fluorescent Mounting Media In-Reply-To: <26143202-3509-4A58-A223-F13DAEEE8CEC@gmail.com> References: <2C9A1D9608959940943F357E0A470FF8B00A370CFF@USCTMXP51005.merck.com> <26143202-3509-4A58-A223-F13DAEEE8CEC@gmail.com> Message-ID: Try www.Valley-Scientific.com Loralei On Tue, Jan 15, 2013 at 10:51 AM, Tina Van Meter wrote: > ProLong Gold (Invitrogen) > > > > Sent from my iPhone > > On Jan 15, 2013, at 1:07 PM, "Suresch, Donna L." > wrote: > > > Hello Histonet, > > Anyone know of a non-aqueous permanent mounting media for use with > fluorescent IHC? > > > > Donna L. Suresch > > Senior Imaging Research Scientist > > Merck Research Laboratories > > Department of Imaging - West Point Campus > > Mail Stop: WP44K Office: WP44-H129 > > 770 Sumneytown Pike > > PO Box 4 > > West Point, PA 19486-0004 > > Phone: 215-652-7349 > > Fax: 215-993-6803 > > > > > > Notice: This e-mail message, together with any attachments, contains > > information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, > > New Jersey, USA 08889), and/or its affiliates Direct contact information > > for affiliates is available at > > http://www.merck.com/contact/contacts.html) that may be confidential, > > proprietary copyrighted and/or legally privileged. It is intended solely > > for the use of the individual or entity named on this message. If you are > > not the intended recipient, and have received this message in error, > > please notify us immediately by reply e-mail and then delete it from > > your system. > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From lldewe <@t> gmail.com Tue Jan 15 13:07:02 2013 From: lldewe <@t> gmail.com (Loralei Dewe) Date: Tue Jan 15 13:07:08 2013 Subject: [Histonet] Permanent Fluorescent Mounting Media In-Reply-To: References: <2C9A1D9608959940943F357E0A470FF8B00A370CFF@USCTMXP51005.merck.com> <26143202-3509-4A58-A223-F13DAEEE8CEC@gmail.com> Message-ID: Oh BTW they will send a free sample out for testing! Loralei On Tue, Jan 15, 2013 at 11:05 AM, Loralei Dewe wrote: > Try www.Valley-Scientific.com > > Loralei > > > On Tue, Jan 15, 2013 at 10:51 AM, Tina Van Meter wrote: > >> ProLong Gold (Invitrogen) >> >> >> >> Sent from my iPhone >> >> On Jan 15, 2013, at 1:07 PM, "Suresch, Donna L." >> wrote: >> >> > Hello Histonet, >> > Anyone know of a non-aqueous permanent mounting media for use with >> fluorescent IHC? >> > >> > Donna L. Suresch >> > Senior Imaging Research Scientist >> > Merck Research Laboratories >> > Department of Imaging - West Point Campus >> > Mail Stop: WP44K Office: WP44-H129 >> > 770 Sumneytown Pike >> > PO Box 4 >> > West Point, PA 19486-0004 >> > Phone: 215-652-7349 >> > Fax: 215-993-6803 >> > >> > >> > Notice: This e-mail message, together with any attachments, contains >> > information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, >> > New Jersey, USA 08889), and/or its affiliates Direct contact information >> > for affiliates is available at >> > http://www.merck.com/contact/contacts.html) that may be confidential, >> > proprietary copyrighted and/or legally privileged. It is intended solely >> > for the use of the individual or entity named on this message. If you >> are >> > not the intended recipient, and have received this message in error, >> > please notify us immediately by reply e-mail and then delete it from >> > your system. >> > _______________________________________________ >> > Histonet mailing list >> > Histonet@lists.utsouthwestern.edu >> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > From AGleiberman <@t> cbiolabs.com Tue Jan 15 13:13:54 2013 From: AGleiberman <@t> cbiolabs.com (Anatoli Gleiberman) Date: Tue Jan 15 13:14:05 2013 Subject: [Histonet] Permanent Fluorescent Mounting Media In-Reply-To: References: <2C9A1D9608959940943F357E0A470FF8B00A370CFF@USCTMXP51005.merck.com> <26143202-3509-4A58-A223-F13DAEEE8CEC@gmail.com> Message-ID: <77BC2EEB6AC66C49AEF794DC98BE314C012312F85D@cbiolabs05.CBiolabs.local> I tried it - got a free sample, liked how it looks (and especially price - two-times less than ProLong Gold)and ordered couple of bottles. Unfortunately they polymerized very fast and it was impossible to revive them. So, we switched back to ProLong. Anatoli Gleiberman, PhD Director of Histopathology Cleveland Biolabs, Inc 73 High Street Buffalo, NY 14203 phone:716-849-6810 ext.354 fax:716-849-6817 e-mail: AGleiberman@cbiolabs.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Loralei Dewe Sent: Tuesday, January 15, 2013 2:07 PM To: Tina Van Meter Cc: histonet@lists.utsouthwestern.edu; Suresch, Donna L. Subject: Re: [Histonet] Permanent Fluorescent Mounting Media Oh BTW they will send a free sample out for testing! Loralei On Tue, Jan 15, 2013 at 11:05 AM, Loralei Dewe wrote: > Try www.Valley-Scientific.com > > Loralei > > > On Tue, Jan 15, 2013 at 10:51 AM, Tina Van Meter wrote: > >> ProLong Gold (Invitrogen) >> >> >> >> Sent from my iPhone >> >> On Jan 15, 2013, at 1:07 PM, "Suresch, Donna L." >> >> wrote: >> >> > Hello Histonet, >> > Anyone know of a non-aqueous permanent mounting media for use with >> fluorescent IHC? >> > >> > Donna L. Suresch >> > Senior Imaging Research Scientist >> > Merck Research Laboratories >> > Department of Imaging - West Point Campus >> > Mail Stop: WP44K Office: WP44-H129 >> > 770 Sumneytown Pike >> > PO Box 4 >> > West Point, PA 19486-0004 >> > Phone: 215-652-7349 >> > Fax: 215-993-6803 >> > >> > >> > Notice: This e-mail message, together with any attachments, >> > contains information of Merck & Co., Inc. (One Merck Drive, >> > Whitehouse Station, New Jersey, USA 08889), and/or its affiliates >> > Direct contact information for affiliates is available at >> > http://www.merck.com/contact/contacts.html) that may be >> > confidential, proprietary copyrighted and/or legally privileged. It >> > is intended solely for the use of the individual or entity named on >> > this message. If you >> are >> > not the intended recipient, and have received this message in >> > error, please notify us immediately by reply e-mail and then delete >> > it from your system. >> > _______________________________________________ >> > Histonet mailing list >> > Histonet@lists.utsouthwestern.edu >> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information. From mpowers <@t> dpspa.com Tue Jan 15 13:44:17 2013 From: mpowers <@t> dpspa.com (Marian Powers) Date: Tue Jan 15 13:44:21 2013 Subject: [Histonet] HPV IHC Message-ID: Hi: Has anyone been successful with validating Biocare's HPV-16 and HPV Cocktail Broad Spectrum (*both for IHC)* on either the Leica Bond System or Ventana Bechmark XT? Thanks, -- Marian From galinadeyneko <@t> yahoo.com Tue Jan 15 14:52:21 2013 From: galinadeyneko <@t> yahoo.com (Galina Deyneko) Date: Tue Jan 15 14:52:25 2013 Subject: [Histonet] (no subject) Message-ID: <1358283141.854.YahooMailClassic@web160203.mail.bf1.yahoo.com> Hi Betsy We use the Thermo Shandon precision Cut paraffin cat. # B1002490 ( Thermo Scientific) Galina Deyneko Novartis, Cambridge, MA ? 617-871-7613 w From gu.lang <@t> gmx.at Tue Jan 15 14:56:04 2013 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Tue Jan 15 14:56:13 2013 Subject: [Histonet] Helicobacter pylori immunocytochemistry - cytoplasmatic staining Message-ID: <000601cdf362$bc8cc8a0$35a659e0$@gmx.at> Hi! I need some help with the interpretation of Hp-IHC. I stained several cases of FFPE gastric biopsies with polyclonal anti Hp from Cell Marque (1:200, ventana benchmark). Some cases showed the nice bacteria and had usually a clean overall background. But some cases showed no bacteria but slight cellular staining of epithelial cells. It was confined on the cells, and usually not all cells in the biopsy had this staining. >From literature I learned, that Hp brings its toxine and other proteins (CagA) into the hostcell. Is it possible, that the polyclonal antibody detects this stuff, although hardly an intact bacterium can be seen? Anybody with similar findings? Thanks in advance Gudrun Lang From rjbuesa <@t> yahoo.com Tue Jan 15 15:06:38 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jan 15 15:06:42 2013 Subject: [Histonet] Helicobacter pylori immunocytochemistry - cytoplasmatic staining In-Reply-To: <000601cdf362$bc8cc8a0$35a659e0$@gmx.at> References: <000601cdf362$bc8cc8a0$35a659e0$@gmx.at> Message-ID: <1358283998.44135.YahooMailNeo@web163101.mail.bf1.yahoo.com> Gudrun: Were the cases to be known as positive, or were you trying to determine just that? If they were known as (+) then the bacteria should be seen outside the cells. If you were trying to determine if they were (+) and you do not see the organisms it is just that those cases were (-), especially if all were stained in a single batch. I think it would be very odd if the organisms are not seen but they generated so much toxin that it went into the cells. Ren? J. From: Gudrun Lang To: Histonet@lists.utsouthwestern.edu Sent: Tuesday, January 15, 2013 3:56 PM Subject: [Histonet] Helicobacter pylori immunocytochemistry - cytoplasmatic staining Hi! I need some help with the interpretation of Hp-IHC. I stained several cases of FFPE gastric biopsies with polyclonal anti Hp from Cell Marque (1:200, ventana benchmark). Some cases showed the nice bacteria and had usually a clean overall background. But some cases showed no bacteria but slight cellular staining of epithelial cells. It was confined on the cells, and usually not all cells in the biopsy had this staining. >From literature I learned, that Hp brings its toxine and other proteins (CagA) into the hostcell. Is it possible, that the polyclonal antibody detects this stuff, although hardly an intact bacterium can be seen? Anybody with similar findings? Thanks in advance Gudrun Lang _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histotalk <@t> yahoo.com Tue Jan 15 16:28:23 2013 From: histotalk <@t> yahoo.com (David Kemler) Date: Tue Jan 15 16:28:27 2013 Subject: [Histonet] Jack Ratliff & Dr. Heiko Richter on HistoTALK Message-ID: <1358288903.94159.YahooMailNeo@web121506.mail.ne1.yahoo.com> Hi Folks - Laser Hisology?! That's what is being?discussed (Part 1) by Jack Ratliff, Ratliff Histology Consultants, Franklin, TN?and Dr. Heiko Richter, ROWIAK Company, Hannover, Germany?on HistoTALK http://www.histotalk.com/. Very interesting...very interesting in deed! This is a definite must hear! Yours, Dave? From thisisann <@t> aol.com Tue Jan 15 17:37:06 2013 From: thisisann <@t> aol.com (Ann Specian) Date: Tue Jan 15 17:37:10 2013 Subject: [Histonet] Voice Recognition Systems Message-ID: <8CFC1905D26BED8-B54-31648@Webmail-m120.sysops.aol.com> I am looking for a voice recognition system for the gross room. It needs to be a system that will interface with our LIS. Does anyone have any recommendations in regard to vendors? Thank you, Ann Specian From cls71877 <@t> gmail.com Tue Jan 15 18:38:45 2013 From: cls71877 <@t> gmail.com (Cristi Rigazio) Date: Tue Jan 15 18:38:53 2013 Subject: [Histonet] Voice Recognition Systems In-Reply-To: <8CFC1905D26BED8-B54-31648@Webmail-m120.sysops.aol.com> References: <8CFC1905D26BED8-B54-31648@Webmail-m120.sysops.aol.com> Message-ID: <6FA8BB2E-539F-4C91-8AD3-AB763D708E98@gmail.com> We use Dragon Speak, our LIS is WindoPath. Cristi Sent from my iPhone On Jan 15, 2013, at 3:37 PM, Ann Specian wrote: > > I am looking for a voice recognition system for the gross room. It needs to be a system that will interface with our LIS. > > Does anyone have any recommendations in regard to vendors? > > Thank you, > Ann Specian > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mmooreht <@t> yahoo.com Wed Jan 16 07:54:03 2013 From: mmooreht <@t> yahoo.com (Michelle Moore) Date: Wed Jan 16 07:54:06 2013 Subject: [Histonet] Voice Recognition Systems In-Reply-To: <8CFC1905D26BED8-B54-31648@Webmail-m120.sysops.aol.com> References: <8CFC1905D26BED8-B54-31648@Webmail-m120.sysops.aol.com> Message-ID: <1358344443.56840.YahooMailNeo@web125104.mail.ne1.yahoo.com> VoiceBrook is an awesome pathology voice recognition system! We use Meditech and it does not require an interface. ? Michelle ________________________________ From: Ann Specian To: histonet@lists.utsouthwestern.edu Sent: Tuesday, January 15, 2013 6:37 PM Subject: [Histonet] Voice Recognition Systems I am looking for a voice recognition system for the gross room.? It needs to be a system that will interface with our LIS. Does anyone have any recommendations in regard to vendors? Thank you, Ann Specian _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mike <@t> pathview.com Wed Jan 16 08:25:42 2013 From: mike <@t> pathview.com (Michael Mihalik) Date: Wed Jan 16 08:28:18 2013 Subject: [Histonet] Voice Recognition Systems In-Reply-To: <8CFC1905D26BED8-B54-31648@Webmail-m120.sysops.aol.com> References: <8CFC1905D26BED8-B54-31648@Webmail-m120.sysops.aol.com> Message-ID: <01d201cdf3f5$6a82b360$3f881a20$@pathview.com> As an LIS vendor I hear this question quite often. I'd like to make an attempt to address this query for the benefit of the group. To start with, if your APLIS uses a version of Microsoft WORD to enter gross, or any other part of the report for that matter, then Dragon should work pretty much out of the box. There is no 'interface' required. Also, if your APLIS is based on the Visual Basic programming language, it will probably work for that as well. The bottom line here is that Dragon Naturally Speaking will work with quite a few commercially available word processors. If you're interested in voice entry, I'd recommend purchasing the most inexpensive version available and installing it on a single PC. Just try it. As to Dragon itself, there are different versions and the price varies dramatically. The basic 'engine' appears to be the same in all versions, but the 'medical' version adds an Anatomic Pathology specific vocabulary and it will let you create 'macros' and a few other things. For our clients that use Dragon, they all use the medical version. A couple more notes: 1. There's a company out there called Voicebrook who 'repackages' Dragon for lack of a better word. Basically they make Dragon even easier to use and they provide excellent support from what I've heard. I have to be honest though. I've heard they can be expensive. I say that not to criticize their marketing strategy, but to warn the smaller labs with smaller budgets who read histonet. 2. Grossing, in particular, is challenging to voice recognition not because the word choice is difficult (In fact, the 'home' version of Dragon might work fairly well here), but because of the environment -- it's just "messy" and to get Dragon to truly work well, you'll have to make some corrections. I've seen Dragon work for grossing, but in those places where Dragon worked well for grossing it was because 'templates' were in significant use. Basically, the user would use Dragon to fill in the blanks. If anyone has any further questions please feel free to contact me offline. We have a 20 pathologist, multihospital client who use Dragon 100% for all their diagnoses. Hence, our experience. PS: Your APLIS vendor may charge a license fee to 'allow' you to use Dragon with their product. You should check with them. We do not charge this fee as there really is no effort on our side to enable this functionality for use throughout our entire LIS. Michael Mihalik PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ann Specian Sent: Tuesday, January 15, 2013 3:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Voice Recognition Systems I am looking for a voice recognition system for the gross room. It needs to be a system that will interface with our LIS. Does anyone have any recommendations in regard to vendors? Thank you, Ann Specian _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Joyce.Weems <@t> emoryhealthcare.org Wed Jan 16 08:35:50 2013 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Wed Jan 16 08:36:01 2013 Subject: [Histonet] Voice Recognition Systems In-Reply-To: <8CFC1905D26BED8-B54-31648@Webmail-m120.sysops.aol.com> References: <8CFC1905D26BED8-B54-31648@Webmail-m120.sysops.aol.com> Message-ID: We use Dragon for micro. We found the gross room took more time editing than transcribing, so we gave up on using it there. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ann Specian Sent: Tuesday, January 15, 2013 6:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Voice Recognition Systems I am looking for a voice recognition system for the gross room. It needs to be a system that will interface with our LIS. Does anyone have any recommendations in regard to vendors? Thank you, Ann Specian _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From gu.lang <@t> gmx.at Wed Jan 16 09:48:27 2013 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Wed Jan 16 09:48:41 2013 Subject: AW: AW: [Histonet] Helicobacter pylori immunocytochemistry - cytoplasmatic staining In-Reply-To: <50F67AE1.7770.0077.1@harthosp.org> References: <000601cdf362$bc8cc8a0$35a659e0$@gmx.at> <50F5955F.7770.0077.1@harthosp.org> <000601cdf3ad$e68107c0$b3831740$@gmx.at> <50F67AE1.7770.0077.1@harthosp.org> Message-ID: <000001cdf400$edc8f580$c95ae080$@gmx.at> It is polymer. No endogenous biotin-problem. The staining looks quiet specific. It is confined to cytoplasm. And there are clear negativ cells beside faintly stained "positiv" cells. I'm reading about the envading features of helicobacter into host cells and about the "injection" of VacA and CagA into the host-cell via pili. Therefore I think, the polyclonal antibody also detects those proteins. But this phenomenon should be a common thing? Also cases are described, where the authors see an infra-nuclear area in gastric mucosa cells, that's metachromatic with Touluidinblue. (I'm not through the whole literature) But in their figures of IHC the intracellular stainings are stronger. Perhaps the bugs change their shape and sit in the cells? Or the positiv cells mean something like: Helicobacter was here. This night I repeat Hp-IHC with longer incubation and amplification to see, if the cellular staining can be enhanced, while the surrounding stays clear. I work at a diploma-thesis with MALT-Lymphoma cases and Hp-detection. And now I came across this "funny" staining. I will also do a PCR on some of these cases. It will be interesting, if this staining is correlated with a positiv PCR. Bye Gudrun -----Urspr?ngliche Nachricht----- Von: Richard Cartun [mailto:Rcartun@harthosp.org] Gesendet: Mittwoch, 16. J?nner 2013 16:03 An: gu.lang@gmx.at Betreff: Re: AW: [Histonet] Helicobacter pylori immunocytochemistry - cytoplasmatic staining Is that avidin-biotin based or polymer? Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-2204 Fax >>> "Gudrun Lang" 1/16/2013 12:54 AM >>> We work with ultraview-dab kit on ventana benchmark Ultra. Gudrun -----Urspr?ngliche Nachricht----- Von: Richard Cartun [mailto:Rcartun@harthosp.org] Gesendet: Dienstag, 15. J?nner 2013 23:44 An: gu.lang@gmx.at Betreff: Re: [Histonet] Helicobacter pylori immunocytochemistry - cytoplasmatic staining Which Ventana detection system are you using? Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-2204 Fax >>> "Gudrun Lang" 1/15/2013 3:56 PM >>> Hi! I need some help with the interpretation of Hp-IHC. I stained several cases of FFPE gastric biopsies with polyclonal anti Hp from Cell Marque (1:200, ventana benchmark). Some cases showed the nice bacteria and had usually a clean overall background. But some cases showed no bacteria but slight cellular staining of epithelial cells. It was confined on the cells, and usually not all cells in the biopsy had this staining. >From literature I learned, that Hp brings its toxine and other proteins (CagA) into the hostcell. Is it possible, that the polyclonal antibody detects this stuff, although hardly an intact bacterium can be seen? Anybody with similar findings? Thanks in advance Gudrun Lang _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Joyce.Weems <@t> emoryhealthcare.org Wed Jan 16 09:56:41 2013 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Wed Jan 16 09:57:14 2013 Subject: AW: [Histonet] Helicobacter pylori immunocytochemistry - cytoplasmatic staining In-Reply-To: <000001cdf400$edc8f580$c95ae080$@gmx.at> References: <000601cdf362$bc8cc8a0$35a659e0$@gmx.at> <50F5955F.7770.0077.1@harthosp.org> <000601cdf3ad$e68107c0$b3831740$@gmx.at> <50F67AE1.7770.0077.1@harthosp.org> <000001cdf400$edc8f580$c95ae080$@gmx.at> Message-ID: Have you checked to see if patient treatment might be a factor? Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph?s Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: Wednesday, January 16, 2013 10:48 AM To: 'Richard Cartun' Cc: histonet@lists.utsouthwestern.edu Subject: AW: AW: [Histonet] Helicobacter pylori immunocytochemistry - cytoplasmatic staining It is polymer. No endogenous biotin-problem. The staining looks quiet specific. It is confined to cytoplasm. And there are clear negativ cells beside faintly stained "positiv" cells. I'm reading about the envading features of helicobacter into host cells and about the "injection" of VacA and CagA into the host-cell via pili. Therefore I think, the polyclonal antibody also detects those proteins. But this phenomenon should be a common thing? Also cases are described, where the authors see an infra-nuclear area in gastric mucosa cells, that's metachromatic with Touluidinblue. (I'm not through the whole literature) But in their figures of IHC the intracellular stainings are stronger. Perhaps the bugs change their shape and sit in the cells? Or the positiv cells mean something like: Helicobacter was here. This night I repeat Hp-IHC with longer incubation and amplification to see, if the cellular staining can be enhanced, while the surrounding stays clear. I work at a diploma-thesis with MALT-Lymphoma cases and Hp-detection. And now I came across this "funny" staining. I will also do a PCR on some of these cases. It will be interesting, if this staining is correlated with a positiv PCR. Bye Gudrun -----Urspr?ngliche Nachricht----- Von: Richard Cartun [mailto:Rcartun@harthosp.org] Gesendet: Mittwoch, 16. J?nner 2013 16:03 An: gu.lang@gmx.at Betreff: Re: AW: [Histonet] Helicobacter pylori immunocytochemistry - cytoplasmatic staining Is that avidin-biotin based or polymer? Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-2204 Fax >>> "Gudrun Lang" 1/16/2013 12:54 AM >>> We work with ultraview-dab kit on ventana benchmark Ultra. Gudrun -----Urspr?ngliche Nachricht----- Von: Richard Cartun [mailto:Rcartun@harthosp.org] Gesendet: Dienstag, 15. J?nner 2013 23:44 An: gu.lang@gmx.at Betreff: Re: [Histonet] Helicobacter pylori immunocytochemistry - cytoplasmatic staining Which Ventana detection system are you using? Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-2204 Fax >>> "Gudrun Lang" 1/15/2013 3:56 PM >>> Hi! I need some help with the interpretation of Hp-IHC. I stained several cases of FFPE gastric biopsies with polyclonal anti Hp from Cell Marque (1:200, ventana benchmark). Some cases showed the nice bacteria and had usually a clean overall background. But some cases showed no bacteria but slight cellular staining of epithelial cells. It was confined on the cells, and usually not all cells in the biopsy had this staining. >From literature I learned, that Hp brings its toxine and other proteins (CagA) into the hostcell. Is it possible, that the polyclonal antibody detects this stuff, although hardly an intact bacterium can be seen? Anybody with similar findings? Thanks in advance Gudrun Lang _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From rsrichmond <@t> gmail.com Wed Jan 16 10:18:30 2013 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Wed Jan 16 10:18:38 2013 Subject: [Histonet] Re: Helicobacter pylori immunocytochemistry - cytoplasmatic staining Message-ID: I quite often see faint cytoplasmic staining in immunostains for Helicobacter. You're supposed to ignore it. It's often present in the negative control slide - one of the few reasons I ever look at a negative control slide. These days I get my IHC for Helicobacter from whatever Genzyme is called this week. I'm noticing considerably less cytoplasmic staining than I've usually seen before. (I have no commercial interest here.) Bob Richmond Samurai Pathologist Maryville TN From LouroP <@t> Princeton.Huntingdon.com Wed Jan 16 12:32:15 2013 From: LouroP <@t> Princeton.Huntingdon.com (Louro, Pedro) Date: Wed Jan 16 12:32:26 2013 Subject: [Histonet] Which fixative for eyes Message-ID: Hello to the eye experts, I am currently working on a large animal (LA) eye project which will give me the flexibility of experimenting with different fixatives. I am currently using Modified Davidson's solution (48 hours) followed by a 10% NBF solution prior to trimming the eyes, embedding , microtomy and H&E staining. After literature research, I found that different labs are using: Modified Davidson's solution Davidson's solution 10% NBF Gluteraldehyde Some places inject the eye with fixative before submerging the eye itself. I wanted to get some ideas / preferences from the experts out there so I can cover my entire basis before finalizing my conclusions. Thanks in advance to all, Pedro Louro Group Leader Histology / IHC ******************************************************************************************** Our Values: Excellence, Drive, Ownership, Challenge, Teamwork, Respect ******************************************************************************************** LEGAL NOTICE This message is confidential and contains information which may be legally privileged. It is intended for the stated addressee(s) only. Access to this email by anyone else is unauthorised. If you are not the intended addressee, any disclosure or copying of the contents of this email or any action taken (or not taken) in reliance on it is unauthorised and is unlawful. If you are not the addressee, please inform the sender immediately and destroy the original message. The data in this email will have been screened for the presence of computer viruses known by the Company at the time the email was produced. The Company cannot guarantee, however, that the email or any attachments are virus free. Delivery of the email will be at the Client's risk. Registered in England No. 1815730 VAT Reg. No. GB425507072 Registered Office: Woolley Road, Alconbury, Huntingdon, Cambridgeshire PE28 4HS ********************************************************************** From JMacDonald <@t> mtsac.edu Wed Jan 16 12:37:32 2013 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Wed Jan 16 12:37:39 2013 Subject: [Histonet] interesting photography Message-ID: A time-lapse movie showing the immune response in the lymph nodes of a mouse edged out a fruit fly sperm fight for top honors at this year's Nikon "Small World in Motion Photomicrography" competition. http://www.scientificamerican.com/video.cfm?id=art-and-science-come-together-in-ni2013-01-15 From lguernsey <@t> ucsd.edu Wed Jan 16 12:59:06 2013 From: lguernsey <@t> ucsd.edu (Lucie Guernsey) Date: Wed Jan 16 12:59:54 2013 Subject: [Histonet] Which fixative for eyes In-Reply-To: References: Message-ID: I'm curious about this as well. We work with mice and rats in our lab and whenever we need eyes (corneas, more specifically), we immersion fix them whole in Bouin's solution overnight at 4C then transfer to 70% EtOH until we're ready to excise the corneas, process and embed into paraffin. We've found that we get nice half-moon sections with good looking stromal and epithelial layers this way compared to fixing the eyes in 4% PFA (which yielded squiggly, damaged corneas). We then perform IHC. Would love to know if there's a better way, even though this seems to work for us. Thanks, Lucie Lucie Guernsey UCSD lguernsey@ucsd.edu On Wed, Jan 16, 2013 at 10:32 AM, Louro, Pedro < LouroP@princeton.huntingdon.com> wrote: > Hello to the eye experts, > > > > I am currently working on a large animal (LA) eye project which will > give me the flexibility of experimenting with different fixatives. > > I am currently using Modified Davidson's solution (48 hours) followed by > a 10% NBF solution prior to trimming the eyes, embedding , microtomy and > H&E staining. > > > > After literature research, I found that different labs are using: > > > > Modified Davidson's solution > > Davidson's solution > > 10% NBF > > Gluteraldehyde > > Some places inject the eye with fixative before submerging the eye > itself. > > > > I wanted to get some ideas / preferences from the experts out there so I > can cover my entire basis before finalizing my conclusions. > > > > Thanks in advance to all, > > > > Pedro Louro > > Group Leader Histology / IHC > > > > > > > > > > ******************************************************************************************** > Our Values: Excellence, Drive, Ownership, Challenge, Teamwork, Respect > > ******************************************************************************************** > > LEGAL NOTICE > This message is confidential and contains information which may be legally > privileged. It is intended for the stated addressee(s) only. Access to this > email by anyone else is unauthorised. If you are not the intended > addressee, any disclosure or copying of the contents of this email or any > action taken (or not taken) in reliance on it is unauthorised and is > unlawful. If you are not the addressee, please inform the sender > immediately and destroy the original message. > The data in this email will have been screened for the presence of > computer viruses known by the Company at the time the email was produced. > The Company cannot guarantee, however, that the email or any attachments > are virus free. Delivery of the email will be at the Client's risk. > Registered in England No. 1815730 > VAT Reg. No. GB425507072 > Registered Office: Woolley Road, Alconbury, Huntingdon, Cambridgeshire > PE28 4HS > ********************************************************************** > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Elizabeth.Cameron <@t> jax.org Wed Jan 16 13:49:11 2013 From: Elizabeth.Cameron <@t> jax.org (Elizabeth Cameron) Date: Wed Jan 16 13:49:17 2013 Subject: [Histonet] Which fixative for eyes In-Reply-To: References: Message-ID: We work with mice and do a variety of fixatives, but we prefer a methanol/acetic acid fixative. It holds the shape of the eye very well, although it may not show the Schlemm's canal as well as other fixatives. -Liz -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lucie Guernsey Sent: Wednesday, January 16, 2013 1:59 PM To: Louro, Pedro Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Which fixative for eyes I'm curious about this as well. We work with mice and rats in our lab and whenever we need eyes (corneas, more specifically), we immersion fix them whole in Bouin's solution overnight at 4C then transfer to 70% EtOH until we're ready to excise the corneas, process and embed into paraffin. We've found that we get nice half-moon sections with good looking stromal and epithelial layers this way compared to fixing the eyes in 4% PFA (which yielded squiggly, damaged corneas). We then perform IHC. Would love to know if there's a better way, even though this seems to work for us. Thanks, Lucie Lucie Guernsey UCSD lguernsey@ucsd.edu On Wed, Jan 16, 2013 at 10:32 AM, Louro, Pedro < LouroP@princeton.huntingdon.com> wrote: > Hello to the eye experts, > > > > I am currently working on a large animal (LA) eye project which will > give me the flexibility of experimenting with different fixatives. > > I am currently using Modified Davidson's solution (48 hours) followed > by a 10% NBF solution prior to trimming the eyes, embedding , > microtomy and H&E staining. > > > > After literature research, I found that different labs are using: > > > > Modified Davidson's solution > > Davidson's solution > > 10% NBF > > Gluteraldehyde > > Some places inject the eye with fixative before submerging the eye > itself. > > > > I wanted to get some ideas / preferences from the experts out there so > I can cover my entire basis before finalizing my conclusions. > > > > Thanks in advance to all, > > > > Pedro Louro > > Group Leader Histology / IHC > > > > > > > > > > ********************************************************************** > ********************** Our Values: Excellence, Drive, Ownership, > Challenge, Teamwork, Respect > > ********************************************************************** > ********************** > > LEGAL NOTICE > This message is confidential and contains information which may be > legally privileged. It is intended for the stated addressee(s) only. > Access to this email by anyone else is unauthorised. If you are not > the intended addressee, any disclosure or copying of the contents of > this email or any action taken (or not taken) in reliance on it is > unauthorised and is unlawful. If you are not the addressee, please > inform the sender immediately and destroy the original message. > The data in this email will have been screened for the presence of > computer viruses known by the Company at the time the email was produced. > The Company cannot guarantee, however, that the email or any > attachments are virus free. Delivery of the email will be at the Client's risk. > Registered in England No. 1815730 > VAT Reg. No. GB425507072 > Registered Office: Woolley Road, Alconbury, Huntingdon, Cambridgeshire > PE28 4HS > ********************************************************************** > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as possible. From BDeBrosse-Serra <@t> isisph.com Thu Jan 17 09:04:37 2013 From: BDeBrosse-Serra <@t> isisph.com (Bea DeBrosse-Serra) Date: Thu Jan 17 09:04:54 2013 Subject: [Histonet] Which fixative for eyes In-Reply-To: References: Message-ID: <493CAA64F203E14E8823737B9EE0E25F092E5BA901@EXCHMB01.isis.local> Davidson's fixative. Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Cameron Sent: Wednesday, January 16, 2013 11:49 AM To: Lucie Guernsey; Louro, Pedro Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Which fixative for eyes We work with mice and do a variety of fixatives, but we prefer a methanol/acetic acid fixative. It holds the shape of the eye very well, although it may not show the Schlemm's canal as well as other fixatives. -Liz -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lucie Guernsey Sent: Wednesday, January 16, 2013 1:59 PM To: Louro, Pedro Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Which fixative for eyes I'm curious about this as well. We work with mice and rats in our lab and whenever we need eyes (corneas, more specifically), we immersion fix them whole in Bouin's solution overnight at 4C then transfer to 70% EtOH until we're ready to excise the corneas, process and embed into paraffin. We've found that we get nice half-moon sections with good looking stromal and epithelial layers this way compared to fixing the eyes in 4% PFA (which yielded squiggly, damaged corneas). We then perform IHC. Would love to know if there's a better way, even though this seems to work for us. Thanks, Lucie Lucie Guernsey UCSD lguernsey@ucsd.edu On Wed, Jan 16, 2013 at 10:32 AM, Louro, Pedro < LouroP@princeton.huntingdon.com> wrote: > Hello to the eye experts, > > > > I am currently working on a large animal (LA) eye project which will > give me the flexibility of experimenting with different fixatives. > > I am currently using Modified Davidson's solution (48 hours) followed > by a 10% NBF solution prior to trimming the eyes, embedding , > microtomy and H&E staining. > > > > After literature research, I found that different labs are using: > > > > Modified Davidson's solution > > Davidson's solution > > 10% NBF > > Gluteraldehyde > > Some places inject the eye with fixative before submerging the eye > itself. > > > > I wanted to get some ideas / preferences from the experts out there so > I can cover my entire basis before finalizing my conclusions. > > > > Thanks in advance to all, > > > > Pedro Louro > > Group Leader Histology / IHC > > > > > > > > > > ********************************************************************** > ********************** Our Values: Excellence, Drive, Ownership, > Challenge, Teamwork, Respect > > ********************************************************************** > ********************** > > LEGAL NOTICE > This message is confidential and contains information which may be > legally privileged. It is intended for the stated addressee(s) only. > Access to this email by anyone else is unauthorised. If you are not > the intended addressee, any disclosure or copying of the contents of > this email or any action taken (or not taken) in reliance on it is > unauthorised and is unlawful. If you are not the addressee, please > inform the sender immediately and destroy the original message. > The data in this email will have been screened for the presence of > computer viruses known by the Company at the time the email was produced. > The Company cannot guarantee, however, that the email or any > attachments are virus free. Delivery of the email will be at the Client's risk. > Registered in England No. 1815730 > VAT Reg. No. GB425507072 > Registered Office: Woolley Road, Alconbury, Huntingdon, Cambridgeshire > PE28 4HS > ********************************************************************** > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as possible. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JGarrigan <@t> cbiolabs.com Thu Jan 17 09:45:24 2013 From: JGarrigan <@t> cbiolabs.com (Jennifer Garrigan) Date: Thu Jan 17 09:45:44 2013 Subject: [Histonet] Fixing and sectioning mouse eye Message-ID: <91BD8268D893C44A823BE2653A60CE53012307C208@cbiolabs05.CBiolabs.local> Hi All, It was recently requested that I section mouse eyes. They were collected in 10% NBF, and then placed in PBS after 48 hours for storage. I cannot find much online with regard to how to proceed. Any suggestions? Are there any special fixation and processing protocols you use? Any information that you could provide would be extremely helpful. Thank you Jennifer Garrigan This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information. From liz <@t> premierlab.com Thu Jan 17 09:50:23 2013 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Thu Jan 17 09:50:28 2013 Subject: [Histonet] RE: Fixing and sectioning mouse eye In-Reply-To: <91BD8268D893C44A823BE2653A60CE53012307C208@cbiolabs05.CBiolabs.local> Message-ID: <14E2C6176416974295479C64A11CB9AE016411ABF785@SBS2K8.premierlab.local> Jennifer It depends on what portion of the eye you are looking at, its this just general tox or a study that is specific for retinopathy Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer Garrigan Sent: Thursday, January 17, 2013 8:45 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Fixing and sectioning mouse eye Hi All, It was recently requested that I section mouse eyes. They were collected in 10% NBF, and then placed in PBS after 48 hours for storage. I cannot find much online with regard to how to proceed. Any suggestions? Are there any special fixation and processing protocols you use? Any information that you could provide would be extremely helpful. Thank you Jennifer Garrigan This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MLashus <@t> pathgroup.com Thu Jan 17 10:04:06 2013 From: MLashus <@t> pathgroup.com (Mighnon Lashus) Date: Thu Jan 17 10:05:23 2013 Subject: [Histonet] Lead Grossing/Histotech Position Chattanooga, TN Message-ID: <58A31EC78E384A4A87410ACF5B3337FB05FBDBB8@PGNEXCHANGE02.pathgroup.com> PathGroup Lab in Chattanooga, TN is in need of an experienced histotech for our 3rd shift Lead Histotech position. I would like the candidate to have experience in a fast paced lab and with performing IHCs using Ventana equipment, supervisory experience would also be helpful. The candidate needs to be a CLIA qualified grossing histotech. If you are interested please contact me by email (mlashus@pathgroup.com) or the phone number listed below. I look forward to hearing from you. Thanks, Mighnon Lashus, HT (ASCP) Laboratory Manager PathGroup 4071 South Access Road, Suite 107 Chattanooga, TN 37406 Phone: 423-493-0207 Fax: 423-493-0208 ________________________________ Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you From histotalk <@t> yahoo.com Thu Jan 17 10:32:21 2013 From: histotalk <@t> yahoo.com (David Kemler) Date: Thu Jan 17 10:32:25 2013 Subject: [Histonet] HistoTALK / Georgia Society for Histotechnology Message-ID: <1358440341.59152.YahooMailNeo@web121503.mail.ne1.yahoo.com> Hello Everyone - ? HistoTALK http://www.histotalk.com/ has been invited to "broadcast" from the GSH Annual Meeting Friday, April 12 - Sunday, April 14th. This is our 2nd year being invited by the GSH and if you have never experienced the kindness, generosity and outstanding capabilities of putting together a AAA meeting - it's time to find out why the GSH is?on my "Top?5" list of?The Best Histology?State Meetings. ? This year it's?on Jekyll Island, GA (5 Star Rating) I know it's going to be an educational blast and a FUN time (as always) for everyone. ? Join HistoTALK this?April?at the Georgia Society for Histotechnology's 2013?Meeting! Their symposium link is on their website found at www.hstosearch.com/gsh/ ? If you want to be guest on the show, let me know! ? Yours, Dave?? From lyonm <@t> upstate.edu Thu Jan 17 10:43:02 2013 From: lyonm <@t> upstate.edu (Michael J. Lyon, Ph.D.) Date: Thu Jan 17 10:43:11 2013 Subject: [Histonet] Paraffin sections mouse brain Message-ID: <002801cdf4d1$b7695c10$263c1430$@upstate.edu> Hi everyone: While I have sectioned a lot of different stuff, I have not done mouse brain before and I am having considerable difficulty. The brains are fixed and were embedded by our pathology department on their automated system. When cutting, the paraffin cuts nicely but the brain tissue shreds as if the infiltration was insufficient. I have re-embedded one, just melted and new paraffin then vacuum oven for 2 hours. The results were the same. I don't know if it would help to re-embed going back to EtOH. Also, from my distant past I recall that when we were paraffin sectioning whole human larynges, that the face of the block was soaked by placing a wet gauze pad on it. I know this help but don't remember what the solution was on the pad. Any help would be appreciated. Thanks Michael J. Lyon, PhD Otolaryngology Research Labs SUNY Upstate Medical University 750 East Adams Street Syracuse, NY 13210 Voice: 315-464-7253 Fax: 315-464-5572 From mucram11 <@t> comcast.net Thu Jan 17 11:09:12 2013 From: mucram11 <@t> comcast.net (Pam Marcum) Date: Thu Jan 17 11:09:20 2013 Subject: [Histonet] Paraffin sections mouse brain In-Reply-To: <002801cdf4d1$b7695c10$263c1430$@upstate.edu> Message-ID: <256083899.8569.1358442552003.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Michael, Do you know or can you get the program they used to process the brain tissue?? Also are they whole or slices of brain (at what thciknees)? Pam Marcum ----- Original Message ----- From: "Michael J. Lyon, Ph.D." To: "Histonet" Sent: Thursday, January 17, 2013 10:43:02 AM Subject: [Histonet] Paraffin sections mouse brain Hi everyone: While I have sectioned a lot of different stuff, I have not done mouse brain before and I am having considerable difficulty. ?The brains are fixed and were embedded by our pathology department on their automated system. ?When cutting, the paraffin cuts nicely but the brain tissue shreds as if the infiltration was insufficient. ?I have re-embedded one, just melted and new paraffin then vacuum oven for 2 hours. ?The results were the same. ?I don't know if it would help to re-embed going back to EtOH. ?Also, from my distant past I recall that when we were paraffin sectioning whole human larynges, that the face of the block was soaked by placing a wet gauze pad on it. ?I know this help but don't remember what the solution was on the pad. ?Any help would be appreciated. ? Thanks ? Michael J. Lyon, PhD Otolaryngology Research Labs SUNY Upstate Medical University 750 East Adams Street Syracuse, NY 13210 ? Voice: 315-464-7253 Fax: ? ? 315-464-5572 ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From 1949rlh <@t> gmail.com Thu Jan 17 11:21:40 2013 From: 1949rlh <@t> gmail.com (Rita) Date: Thu Jan 17 11:21:48 2013 Subject: [Histonet] Cpt code 88305 Message-ID: <4140C032-E482-45E2-A755-1545D0A66916@gmail.com> Sorry wrong code it is 88305 I need to know where to get information Thanks Rita Sent from my iPhone From ihcman2010 <@t> hotmail.com Thu Jan 17 11:52:17 2013 From: ihcman2010 <@t> hotmail.com (Glen Dawson) Date: Thu Jan 17 11:52:25 2013 Subject: [Histonet] MOHs Training In-Reply-To: References: , , Message-ID: From: ihcman2010@hotmail.com To: histonet-request@lists.utsouthwestern.edu Subject: FW: MOHs Training Date: Thu, 17 Jan 2013 07:18:28 -0600 All, My lab is in the process of aiding one of our hospital's sites with MOHs and I'd like to find out what a MOHs lab might charge for training our techs if there was such a service available? What might that process be? How long should it take to get a good histotech with no MOHs experience up and running? What do other labs' training processes look like compared to ours? Is there any place that trains outside techs for such a circumstance? Do you have national contacts that may know someone who knows someone, etc.? Any answers to any/all of the questions above would be an enormous help and would be greatly appreciated. Thank-you in Advance, Glen Dawson BS, HT(ASCP), QIHC Histology Technical Specialist Mercy Health System Janesville, WI From Chris <@t> bakopathology.com Thu Jan 17 12:13:29 2013 From: Chris <@t> bakopathology.com (Chris Winans) Date: Thu Jan 17 12:13:38 2013 Subject: [Histonet] Thigh tissue Message-ID: Hi, I am currently cutting 3 mm thigh punches on a freezing, sliding microtome using a wedge blade. This tissue is the only one that gives us bad sections. We currently use a mixture of dry ice and 30% Sucrose. If anyone has any tips or tricks on this subject it would be greatly appreciated. Thanks in advance.Chris ENFD Supervisor Bako Pathology Services Chris@bakopathology.com From CDavis <@t> che-east.org Thu Jan 17 12:10:27 2013 From: CDavis <@t> che-east.org (Davis, Cassie) Date: Thu Jan 17 12:14:49 2013 Subject: [Histonet] Mouse brains Message-ID: <08861B9CF6C7774E874635A4818AE37B05483334@CHEXCMS01.one.ads.che.org> Michael, we used to cut human brains on a room temp water bath at a hospital I worked at many years ago. It might work for mouse brains too. Cassie Davis ----- Original Message ----- From: "Michael J. Lyon, Ph.D." To: "Histonet" Sent: Thursday, January 17, 2013 10:43:02 AM Subject: [Histonet] Paraffin sections mouse brain Hi everyone: While I have sectioned a lot of different stuff, I have not done mouse brain before and I am having considerable difficulty. ??The brains are fixed and were embedded by our pathology department on their automated system. ??When cutting, the paraffin cuts nicely but the brain tissue shreds as if the infiltration was insufficient. ??I have re-embedded one, just melted and new paraffin then vacuum oven for 2 hours. ??The results were the same. ??I don't know if it would help to re-embed going back to EtOH. ??Also, from my distant past I recall that when we were paraffin sectioning whole human larynges, that the face of the block was soaked by placing a wet gauze pad on it. ??I know this help but don't remember what the solution was on the pad. ??Any help would be appreciated. ?? Thanks ?? Michael J. Lyon, PhD Otolaryngology Research Labs SUNY Upstate Medical University 750 East Adams Street Syracuse, NY 13210 ?? Voice: 315-464-7253 Fax: ?? ?? 315-464-5572 ?? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 12 Date: Thu, 17 Jan 2013 11:21:40 -0600 From: Rita <1949rlh@gmail.com> Subject: [Histonet] Cpt code 88305 To: "histonet@lists.utsouthwestern.edu" Message-ID: <4140C032-E482-45E2-A755-1545D0A66916@gmail.com> Content-Type: text/plain; charset=us-ascii Sorry wrong code it is 88305 I need to know where to get information Thanks Rita Sent from my iPhone ------------------------------ Message: 13 Date: Thu, 17 Jan 2013 11:52:17 -0600 From: Glen Dawson Subject: [Histonet] MOHs Training To: histonet Message-ID: Content-Type: text/plain; charset="iso-8859-1" From: ihcman2010@hotmail.com To: histonet-request@lists.utsouthwestern.edu Subject: FW: MOHs Training Date: Thu, 17 Jan 2013 07:18:28 -0600 All, My lab is in the process of aiding one of our hospital's sites with MOHs and I'd like to find out what a MOHs lab might charge for training our techs if there was such a service available? What might that process be? How long should it take to get a good histotech with no MOHs experience up and running? What do other labs' training processes look like compared to ours? Is there any place that trains outside techs for such a circumstance? Do you have national contacts that may know someone who knows someone, etc.? Any answers to any/all of the questions above would be an enormous help and would be greatly appreciated. Thank-you in Advance, Glen Dawson BS, HT(ASCP), QIHC Histology Technical Specialist Mercy Health System Janesville, WI ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 110, Issue 23 ***************************************** Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From jhill <@t> vet.k-state.edu Thu Jan 17 12:52:38 2013 From: jhill <@t> vet.k-state.edu (Jennifer Hill) Date: Thu Jan 17 12:52:43 2013 Subject: [Histonet] NexES IHC Stainer Message-ID: <8AA2173DC209CA438077A832FF98BD7F07BED9C0@VETMXHT.ads.vet.k-state.edu> I have a Ventana NexES IHC stainer available to anyone who will pay to have it shipped to them. The machine is in working condition, but it is obsolete and Ventana no services/is able to repair them. It could serve a source for parts if you're still using this platform. It does not include the wash and coverslip bottles. Please connect me off list if you are interested. Jennifer Hill Research Assistant Kansas State University Veterinary Diagnostic Lab From rsrichmond <@t> gmail.com Thu Jan 17 13:56:23 2013 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Thu Jan 17 13:56:27 2013 Subject: [Histonet] Re: Fixative for eyes Message-ID: I'm not much familiar with eye pathology or histology, but let's get Davidson's fixative right. Called "modified Davidson's fixative" in papers published more than 50 years ago, it's 3 parts water 3 parts alcohol 2 parts 37% formaldehyde ("strong formalin", not NBF) 1 part glacial acetic acid No glutaraldehyde. I can provide more information if you need it. Dick Dubielzig (pronounced dooBILLzig) is a veterinary eye pathologist in Madison WI who can probably help. If you can't locate him, I can contact him - he's an old friend of mine. Bob Richmond Samurai Pathologist Maryville TN From TNMayer <@t> mdanderson.org Thu Jan 17 14:01:29 2013 From: TNMayer <@t> mdanderson.org (Mayer,Toysha N) Date: Thu Jan 17 14:02:35 2013 Subject: [Histonet] RE: Histonet Digest, Vol 110, Issue 23 In-Reply-To: <63a7817f-c673-487c-b5b1-53140f0dc1ed@DCPWPRTR02.mdanderson.edu> References: <63a7817f-c673-487c-b5b1-53140f0dc1ed@DCPWPRTR02.mdanderson.edu> Message-ID: <47E9B2C01DDDD94881EACD2DC44EBC880239E5@D1PWPEXMBX05.mdanderson.edu> Try Annetta Walker. Here is her website info. http://burnett-walkerlabs.com/ She has been doing MOHS for years and started training and setup professionally. Good luck. Toysha N. Mayer, MBA, HT (ASCP) Instructor, Education Coordinator Program in Histotechnology School of Health Professions MD Anderson Cancer Center (713) 563-3481 tnmayer@mdanderson.org ------------------------------ Message: 13 Date: Thu, 17 Jan 2013 11:52:17 -0600 From: Glen Dawson Subject: [Histonet] MOHs Training To: histonet Message-ID: Content-Type: text/plain; charset="iso-8859-1" From: ihcman2010@hotmail.com To: histonet-request@lists.utsouthwestern.edu Subject: FW: MOHs Training Date: Thu, 17 Jan 2013 07:18:28 -0600 All, My lab is in the process of aiding one of our hospital's sites with MOHs and I'd like to find out what a MOHs lab might charge for training our techs if there was such a service available? What might that process be? How long should it take to get a good histotech with no MOHs experience up and running? What do other labs' training processes look like compared to ours? Is there any place that trains outside techs for such a circumstance? Do you have national contacts that may know someone who knows someone, etc.? Any answers to any/all of the questions above would be an enormous help and would be greatly appreciated. Thank-you in Advance, Glen Dawson BS, HT(ASCP), QIHC Histology Technical Specialist Mercy Health System Janesville, WI ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 110, Issue 23 ***************************************** From histotalk <@t> yahoo.com Thu Jan 17 14:02:42 2013 From: histotalk <@t> yahoo.com (David Kemler) Date: Thu Jan 17 14:02:50 2013 Subject: [Histonet] Ooops! Wrong Link! HistoTALK / Georgia Society for Histotechnology In-Reply-To: <1358440341.59152.YahooMailNeo@web121503.mail.ne1.yahoo.com> References: <1358440341.59152.YahooMailNeo@web121503.mail.ne1.yahoo.com> Message-ID: <1358452962.779.YahooMailNeo@web121504.mail.ne1.yahoo.com> Hello Everyone - ? HistoTALK http://www.histotalk.com/ has been invited to "broadcast" from the GSH Annual Meeting Friday, April 12 - Sunday, April 14th. This is our 2nd year being invited by the GSH and if you have never experienced the kindness, generosity and outstanding capabilities of putting together a AAA meeting - it's time to find out why the GSH is?on my "Top?5" list of?The Best Histology?State Meetings. ? This year it's?on Jekyll Island, GA (5 Star Rating) I know it's going to be an educational blast and a FUN time (as always) for everyone. ? Join HistoTALK this?April?at the Georgia Society for Histotechnology's 2013?Meeting! Their symposium link is on their website found at www.histosearch.com/gsh/ ? If you want to be guest on the show, let me know! ? Yours, Dave?? From sdysart <@t> mirnarx.com Thu Jan 17 14:06:19 2013 From: sdysart <@t> mirnarx.com (Sarah Dysart) Date: Thu Jan 17 14:06:34 2013 Subject: [Histonet] Re: Fixative for eyes In-Reply-To: References: Message-ID: <8A70A9B2ECDD084DACFE6C59FCF86D5047B55E52@BL2PRD0710MB363.namprd07.prod.outlook.com> Don't know what the original post was about but...this is the recipe I have been using for 10+ years that was passed on to me from a veteran HT...it works GREAT on eye histology!! Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Thursday, January 17, 2013 1:56 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Fixative for eyes I'm not much familiar with eye pathology or histology, but let's get Davidson's fixative right. Called "modified Davidson's fixative" in papers published more than 50 years ago, it's 3 parts water 3 parts alcohol 2 parts 37% formaldehyde ("strong formalin", not NBF) 1 part glacial acetic acid No glutaraldehyde. I can provide more information if you need it. Dick Dubielzig (pronounced dooBILLzig) is a veterinary eye pathologist in Madison WI who can probably help. If you can't locate him, I can contact him - he's an old friend of mine. Bob Richmond Samurai Pathologist Maryville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From yyung <@t> scripps.edu Thu Jan 17 15:06:07 2013 From: yyung <@t> scripps.edu (Yun Yung) Date: Thu Jan 17 15:05:40 2013 Subject: [Histonet] WANTED: Leica cryostat 50 mm anti-roll plate assembly Message-ID: <9EB1B123C28EA4488A6D524DAF09F32D01D60D22674D@EXCH-CCR01.lj.ad.scripps.edu> Dear Histonet, Would anyone have a spare 50 mm anti-roll plate assembly (without glass is fine) that they would like to sell? We have an older Jung-Reichart Frigocut that could use part replaced. I've attached a picture of the part. Many thanks in advance, Yun ___________________________________________ Yun C. Yung, Ph.D. Hydrocephalus Association Mentored Young Investigator Chun Lab, Dorris Neuroscience Center The Scripps Research Institute 10550 N. Torrey Pines Rd, DNC-118 La Jolla, CA 92037 Email: yyung@scripps.edu Tel: 858.784.7041 From JMacDonald <@t> mtsac.edu Thu Jan 17 16:30:59 2013 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Thu Jan 17 16:31:07 2013 Subject: [Histonet] block storage Message-ID: Does anyone have printed guidelines for temperature for storing paraffin blocks? I know common sense guidelines, but is there an official document? Thanks, Jennifer From tahseen <@t> brain.net.pk Thu Jan 17 22:06:22 2013 From: tahseen <@t> brain.net.pk (tahseen@brain.net.pk) Date: Thu Jan 17 22:06:32 2013 Subject: [Histonet] Which fixative for eyes In-Reply-To: References: Message-ID: <28548.203.135.35.66.1358481982.squirrel@brain.net.pk> Dear Pedro Louro We are using Alcoholic formalin fixative for ocular (Eye) specimens, Reference Tech Sample (ASAP) HT-2(1995), since last 19 year without facing any problem. Composition: Ethanol absolute 90 ml Formaldehyde con 10 ml Fix for 24 hours Thanks, Muhammad Tahseen Histology Supervisor SKMCH&RC Lahore Pakistan > Hello to the eye experts, > > > > I am currently working on a large animal (LA) eye project which will > give me the flexibility of experimenting with different fixatives. > > I am currently using Modified Davidson's solution (48 hours) followed by > a 10% NBF solution prior to trimming the eyes, embedding , microtomy and > H&E staining. > > > > After literature research, I found that different labs are using: > > > > Modified Davidson's solution > > Davidson's solution > > 10% NBF > > Gluteraldehyde > > Some places inject the eye with fixative before submerging the eye > itself. > > > > I wanted to get some ideas / preferences from the experts out there so I > can cover my entire basis before finalizing my conclusions. > > > > Thanks in advance to all, > > > > Pedro Louro > > Group Leader Histology / IHC > > > > > > > > > ******************************************************************************************** > Our Values: Excellence, Drive, Ownership, Challenge, Teamwork, Respect > ******************************************************************************************** > > LEGAL NOTICE > This message is confidential and contains information which may be legally > privileged. It is intended for the stated addressee(s) only. Access to > this email by anyone else is unauthorised. If you are not the intended > addressee, any disclosure or copying of the contents of this email or any > action taken (or not taken) in reliance on it is unauthorised and is > unlawful. If you are not the addressee, please inform the sender > immediately and destroy the original message. > The data in this email will have been screened for the presence of > computer viruses known by the Company at the time the email was produced. > The Company cannot guarantee, however, that the email or any attachments > are virus free. Delivery of the email will be at the Client's risk. > Registered in England No. 1815730 > VAT Reg. No. GB425507072 > Registered Office: Woolley Road, Alconbury, Huntingdon, Cambridgeshire > PE28 4HS > ********************************************************************** > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From ccrowder <@t> vetmed.lsu.edu Fri Jan 18 09:34:43 2013 From: ccrowder <@t> vetmed.lsu.edu (Cheryl Crowder) Date: Fri Jan 18 09:34:53 2013 Subject: [Histonet] Horse eye fixation Message-ID: Pedro - Since horse eyes are so large and dense, if possible the eye should be fenestrated to allow the fixative to penetrate the interior. Injection is also and option. Davidson's is the preferred fixative, as opposed to Bouin's, as it is fast penetrating and less hazardous. After initial fixation with Davidson's (or modified Davidson's), place the eye in 70% alcohol overnight. There is no need to place the eye in formalin after the Davidson's fixation. Cheryl Crowder, BA, HTL(ASCP) Crowder Histology Consulting 4952 Alvin Dark Ave. Baton Rouge, LA 70820 (225) 772-2865 From twheelock <@t> mclean.harvard.edu Fri Jan 18 13:17:35 2013 From: twheelock <@t> mclean.harvard.edu (Tim Wheelock) Date: Fri Jan 18 13:17:45 2013 Subject: [Histonet] Laboratory SOP Message-ID: <50F99FCF.10600@mclean.harvard.edu> Hi Everyone: I am putting together an SOP for my neuropathology lab. Does anyone know of a template, example, or guide that I could refer to? Thanks, Tim Wheelock Harvard Brain Bank McLean Hospital Belmont, MA From rosenfeldtek <@t> hotmail.com Fri Jan 18 13:48:49 2013 From: rosenfeldtek <@t> hotmail.com (Jerry Ricks) Date: Fri Jan 18 13:48:54 2013 Subject: [Histonet] World's most expensive microtome and I want it Message-ID: It's based on a near infrared femtosecond laser. http://www.rowiak.de/index.php?id=30&tx_ttnews[tt_news]=9&cHash=5e8f97b30bbbd9908c62c3fbb67ba312 http://adsabs.harvard.edu/abs/2007SPIE.6460E...3W From Herrick.James <@t> mayo.edu Fri Jan 18 13:51:20 2013 From: Herrick.James <@t> mayo.edu (Herrick, James L. (Jim)) Date: Fri Jan 18 13:51:26 2013 Subject: [Histonet] SRBS/van Gieson Message-ID: <669007BC9D903242957AF72E783646A6035E6E@MSGPEXCHA09A.mfad.mfroot.org> Happy Friday Everybody, We are trying to develop a protocol for an SRBS/van Gieson's stain on MMA embedded tissue. The sections are 5um in thickness and deplasticized. Our protocol seems to work ok on thick sections but not on thin, deplasticized sections. Any thoughts or ideas would be greatly appreciated. Hope you all have a great weekend!! Thanks, Jim From ratliffjack <@t> hotmail.com Fri Jan 18 14:17:29 2013 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Fri Jan 18 14:17:38 2013 Subject: [Histonet] SRBS/van Gieson In-Reply-To: <669007BC9D903242957AF72E783646A6035E6E@MSGPEXCHA09A.mfad.mfroot.org> References: <669007BC9D903242957AF72E783646A6035E6E@MSGPEXCHA09A.mfad.mfroot.org> Message-ID: What is it that you specifically want to accomplish that you can't do with a VonKossa/MacNeals Tetrachrome or Goldner's Trichrome Stain? Jack On Jan 18, 2013, at 1:51 PM, "Herrick, James L. (Jim)" wrote: > Happy Friday Everybody, > > We are trying to develop a protocol for an SRBS/van Gieson's stain on MMA embedded tissue. The sections are 5um in thickness and deplasticized. Our protocol seems to work ok on thick sections but not on thin, deplasticized sections. Any thoughts or ideas would be greatly appreciated. Hope you all have a great weekend!! > > Thanks, > Jim > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Ronald.Houston <@t> nationwidechildrens.org Fri Jan 18 21:39:32 2013 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Fri Jan 18 21:39:38 2013 Subject: [Histonet] HT vacancy Message-ID: We have an immediate opening for a registered Histotechnician at Nationwide Childrens Hospital in Columbus, OH. Nationwide Childrens Hospital is the second largest free-standing pediatric facility in the nation. We offer a competitive salary and relocation allowance. We are looking for an exemplary team player with a sound knowledge, both theoretical and practical, of microtomy, cryotomy, special stains, IHC, IF and ISH. Experience in electron microscopy a bonus, but full training will be provided in this and other specialties peculiar to pediatric pathology. For more information and consideration, please contact me off-line or apply at www.nationwidechildrens.org Thanks Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org From kdwyer3322 <@t> aol.com Sat Jan 19 17:41:45 2013 From: kdwyer3322 <@t> aol.com (kdwyer3322@aol.com) Date: Sat Jan 19 17:41:52 2013 Subject: [Histonet] Texas Society for Histotechnology 2013 Symosium/Convention Message-ID: <8CFC4B5AD19DD92-51A0-13B67@webmail-m155.sysops.aol.com> Solving the Puzzle???. ??..one Piece at a Time ??..one Piece at a Time All, The Texas Society for Histotechnology will be having the 2013 Symposium/Convention at the: J.W. Marriott 5150 Westheimer Houston, TX 77506-5506 713-961-1500 April 12-14, 2013 If you would like more information or a copy of the program please contact: Kathy Dwyer kdwyer3322@aol.com Veronica Davis veronida@baylorhealth.edu veronida@baylorhealth.edu If you would like more information or a copy of the program please contact: Kathy Dwyer kdwyer3322@aol.com Veronica Davis veronida@baylorhealth.edu veronida@baylorhealth.edu From kdwyer3322 <@t> aol.com Sat Jan 19 19:16:23 2013 From: kdwyer3322 <@t> aol.com (Kathy Dwyer) Date: Sat Jan 19 19:16:32 2013 Subject: [Histonet] UPDATED: Texas Society For Histotechnology 2013 Symposium/Convention References: <8CFC4B5AD19DD92-51A0-13B67@webmail-m155.sysops.aol.com> Message-ID: <174847E1-0E52-4F1B-BC81-452353099AB8@aol.com> > All, > The Texas Society for Histotechnology will be having the 2013 Symposium/Convention at the: > > J.W. Marriott > 5150 Westheimer > Houston, TX > 77506-5506 > 713-961-1500 > April 12-14, 2013 > > > If you would like more information or a copy of the program please contact: > > Kathy Dwyer > kdwyer3322@aol.com > > Veronica Davis > veronida@baylorhealth.edu > > > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amosbrooks <@t> gmail.com Sun Jan 20 08:13:23 2013 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Sun Jan 20 08:13:26 2013 Subject: [Histonet] Re: Histonet Digest, Vol 110, Issue 25 In-Reply-To: <50fadf41.4a9fb60a.6237.ffff8345SMTPIN_ADDED_MISSING@mx.google.com> References: <50fadf41.4a9fb60a.6237.ffff8345SMTPIN_ADDED_MISSING@mx.google.com> Message-ID: Hi Jim, If I were to guess (which is essentially what one needs to do in the absence of a procedure) I would say that since the thick sections work but thin do not it is likely being over differentiated. This is why most procedures do not specify a specific time in the Ferric chloride. It totally depends on the tissue. Dense elastic tissue takes longer to differentiate than fine delicate fibers, and thick sections take longer than thin to differentiate. So if the thick and thin sections are differentiated for the same time the thin will end up over differentiated and possibly totally de-stained if left in long enough to get good differentiation of the thick sections. This is one of the reasons I love this stain. I call it job security since automating subtle differences is near impossible and it takes some knowledge of histology and the staining chemistry to do this properly. Best of luck, Amos Brooks On Sat, Jan 19, 2013 at 1:00 PM, wrote: > Message: 3 > Date: Fri, 18 Jan 2013 19:51:20 +0000 > From: "Herrick, James L. (Jim)" > Subject: [Histonet] SRBS/van Gieson > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > < > 669007BC9D903242957AF72E783646A6035E6E@MSGPEXCHA09A.mfad.mfroot.org> > Content-Type: text/plain; charset="us-ascii" > > Happy Friday Everybody, > > We are trying to develop a protocol for an SRBS/van Gieson's stain on MMA > embedded tissue. The sections are 5um in thickness and deplasticized. Our > protocol seems to work ok on thick sections but not on thin, deplasticized > sections. Any thoughts or ideas would be greatly appreciated. Hope you all > have a great weekend!! > > Thanks, > Jim > From Chris <@t> bakopathology.com Mon Jan 21 06:59:45 2013 From: Chris <@t> bakopathology.com (Chris Winans) Date: Mon Jan 21 06:59:51 2013 Subject: [Histonet] Question removal Message-ID: Hello, How do I go about removing a question from this site? Thanks! Chris ENFD Supervisor Bako Pathology Services Chris@bakopathology.com From rjbuesa <@t> yahoo.com Mon Jan 21 09:10:15 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Jan 21 09:10:39 2013 Subject: [Histonet] Question removal In-Reply-To: References: Message-ID: <1358781015.78636.YahooMailNeo@web163104.mail.bf1.yahoo.com> As in any e-mail, once you press "send", you cannot withdraw/remove the message. You may try to write to the master of the site. Ren? J. From: Chris Winans To: "Histonet@lists.utsouthwestern.edu" Sent: Monday, January 21, 2013 7:59 AM Subject: [Histonet] Question removal Hello, How do I go about removing a question from this site? Thanks! Chris ENFD Supervisor Bako Pathology Services Chris@bakopathology.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Dorothy.L.Webb <@t> HealthPartners.Com Mon Jan 21 11:58:33 2013 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Mon Jan 21 11:58:37 2013 Subject: [Histonet] brain tissue Message-ID: <65365F35C0F2EF4D846EC3CA73E49C430227AFFBCC2F@HPEMX3.HealthPartners.int> How is everyone processing brain tissue from brain surgery, not necessarily a small bx? Does anyone have a separately timed process for such tissue to get them out faster? And what is the minimum fixation time suggested? Thanks, Dorothy Webb ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 From Linda.Margraf <@t> cookchildrens.org Mon Jan 21 13:39:51 2013 From: Linda.Margraf <@t> cookchildrens.org (Linda Margraf) Date: Mon Jan 21 13:39:55 2013 Subject: [Histonet] RE: Histonet Digest, Vol 110, Issue 27 In-Reply-To: References: Message-ID: <928719B9EBFA1C4686918B975FF84528C7843449@CCHCSMBX04.CCHCS.LDAP> In response to this message....... Hello, How do I go about removing a question from this site? Thanks! Chris ENFD Supervisor Bako Pathology Services Chris@bakopathology.com Dear Chris, It is very hard to get a submission removed from the Histonet list, unfortunately. There are websites that archive the list (in addition to the official Histosearch Histonet archive and the University Histonet server) so even if these were removed, the message would still be out there on the internet. If it is a critical matter we can look into seeing what can be done, but in the past, we have been unable to remove messages from the list. Sorry, Linda M Histonet administrator From rjbuesa <@t> yahoo.com Mon Jan 21 14:44:52 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Jan 21 14:44:55 2013 Subject: [Histonet] brain tissue In-Reply-To: <65365F35C0F2EF4D846EC3CA73E49C430227AFFBCC2F@HPEMX3.HealthPartners.int> References: <65365F35C0F2EF4D846EC3CA73E49C430227AFFBCC2F@HPEMX3.HealthPartners.int> Message-ID: <1358801092.15593.YahooMailNeo@web163103.mail.bf1.yahoo.com> Brain tissues require more time to be fixed correctly so, at least, 48 hours fixation. They also require a complete/perfect infiltration so do not even think in processing them as "ordinary" surgical specimens are. They will require at least to be processed as breast are. I used to have a separate machine/protocol for brains. Ren? J. From: "Webb, Dorothy L" To: "'histonet@lists.utsouthwestern.edu'" Sent: Monday, January 21, 2013 12:58 PM Subject: [Histonet] brain tissue How is everyone processing brain tissue from brain surgery, not necessarily a small bx?? Does anyone have a separately timed process for such tissue to get them out faster?? And what is the minimum fixation time suggested? Thanks,? Dorothy Webb ? ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ewj <@t> pigsqq.org Mon Jan 21 16:19:03 2013 From: ewj <@t> pigsqq.org (=?UTF-8?B?IkUuIFdheW5lIEpvaG5zb24g5pyx56iz5qOuIg==?=) Date: Mon Jan 21 16:19:23 2013 Subject: [Histonet] Question removal In-Reply-To: References: Message-ID: <50FDBED7.7000404@pigsqq.org> It's not exactly like dewaxing your slides. It's more like un-waxing them. Some have tried downloading the Zen module to unask their questions but it is only supported on XP and no longer available. With UNIX you can just type >unask -all -q See unask -man for documentation. The un-answering feature is still under development for LISTSERV but is expected in a future release. We tried reversing the polarity on the answering machine in our lab but it kept eating the tape. On 3:59, Chris Winans wrote: > Hello, > How do I go about removing a question from this site? Thanks! > Chris > > ENFD Supervisor > Bako Pathology Services > Chris@bakopathology.com > > > > From aakrasht <@t> yahoo.com Mon Jan 21 17:22:00 2013 From: aakrasht <@t> yahoo.com (Ali A Krasht) Date: Mon Jan 21 17:22:04 2013 Subject: [Histonet] Seeking a Supervisory/ Histologist Position Message-ID: <1358810520.77614.YahooMailNeo@web124701.mail.ne1.yahoo.com> Hi ABOUT ME I am a Histologist (HT & HTL ASCP Approved). I am currently located in Frisco Texas. I graduated with a B.S. in Biology with a minor in Psychology and two years Medical Technology including a part time Internet Marketing.? I have ten years of Histology experience/ supervisor including grossing, IHC and IF. I also have sales and marketing experience. I opened and setup Omnipath Diagnostics Texas and recently consult with a doctor in California in opening a private laboratory. I have worked in both large and small laboratories, in pharmaceuticals testing, reference and clinical environments.? I am seeking a consultant, supervisory/Histologist or sales/marketing in an organization with a team mentality and opportunities to use my skills to a greater degree and would love an opportunity for growth and development.? Please take a look at my LinkedIn Profile if you need to know more: www.linkedin.com/in/alikrasht/ ? Ali A. Krasht http://NovellTrade.com http://NovoJeans.com 214 444 8319 ************************************************ The information transmitted is intended only for the person or entity to which it is addressed and may contain proprietary, business-confidential and/or privileged material. If you are not the intended recipient of this message you are hereby notified that any use, review, retransmission, dissemination, distribution, reproduction or any action taken in reliance upon this message is prohibited. If you received this in error, please contact the sender and delete the material from any computer. Any views expressed in this message are those of the individual sender and may not necessarily reflect the views of the company. ************************************************ From lyonm <@t> upstate.edu Tue Jan 22 08:09:16 2013 From: lyonm <@t> upstate.edu (Michael J. Lyon, Ph.D.) Date: Tue Jan 22 08:09:35 2013 Subject: [Histonet] Thanks Message-ID: <005001cdf8aa$1082d1b0$31887510$@upstate.edu> I want to thank all of those that responded to my question so quickly. Problem solved!!! This is a great list with lots of helpful people. Michael J. Lyon, PhD Otolaryngology Research Labs SUNY Upstate Medical University 750 East Adams Street Syracuse, NY 13210 Voice: 315-464-7253 Fax: 315-464-5572 From relia1 <@t> earthlink.net Tue Jan 22 09:10:58 2013 From: relia1 <@t> earthlink.net (Pam Barker) Date: Tue Jan 22 09:11:02 2013 Subject: [Histonet] RELIA Histology Careers Bulletin 1/22/2013 Brand New Opportunities!! Message-ID: <006c01cdf8b2$af250b00$0d6f2100$@earthlink.net> Hi Histonetters!! Right Place, Right Time, Right Opportunity. Sometimes your next career move is just a matter of being in the right place at the right time and ready for the right opportunity. Odds are that if you are contemplating a job change for whatever reason - better compensation, a more desirable location or more challenging work, you don't have the time to do a job search. Let me help you with that. I have clients located throughout the country. All of the jobs that I represent are full time permanent positions with excellent compensation, benefits and relocation/sign-on bonuses. If you are ASCP certified or eligible my clients are interested in speaking with you! I will assist you with your resume, coordination of interviews and coaching throughout the process. Remember my services are FREE of charge to you. Here is the latest update of the positions I am most excited to represent. If you are interested in any of these positions or a position like anyone of these but in another area either way - give me a call toll free at 866-607-3542 or shoot me an e-mail at relia1@earthlink.net and let's discuss it. Whether you are looking for a new position today, tomorrow or 6 months from now, it is never too early to have me keep a watch out for that perfect job for you! *These are our brand new opportunities* Pathology Manager - San Francisco Bay Prestigious academic organization!! Histotech - San Mateo, CA State of the Art Lab great compensation!! Histotechnician - East of Columbus WILL CONSIDER ENTRY LEVEL TECHS!!! Histology Tech - Staunton, VA Opportunity To train histology students!! HT/HTL Certified - Lancaster, PA Learn to gross, state of the art lab!! Histology Tech - Louisville, KY - Great team to work with excellent bennies!! Histotechnician/Histotechnologist-Concord, NH Great bennies and team env.!! Histologist and FISH Tech - Long Island State of the Art Lab great $$ Remember if nothing sounds interesting on my current list you can pass it on to your friends and wait for the next one OR give me a call or shoot me an e-mail telling me what you are looking for in your next opportunity. Remember timing is everything. Also, if you know someone who might be interested I offer a$500 referral bonus. There are a lot of recruiters out there right now trying to work with histo techs and I appreciate your support and respect your needs. Remember I offer over 25 years of experience as a recruiter and for over 10 years I have dedicated my practice solely to placing histology professionals like you. Thanks - Pam 866-60-RELIA (866-607-3542) Happy New Year!! Thank You! Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From BZIMMERM <@t> gru.edu Tue Jan 22 09:40:05 2013 From: BZIMMERM <@t> gru.edu (Zimmerman, Billie) Date: Tue Jan 22 09:40:11 2013 Subject: [Histonet] Georgia Society for Histotechnology Message-ID: <7B3DEB32E69C034EACB479059C5DE3FF751BFD@EX-MLB-03.ad.georgiahealth.edu> GSH will be celebrating their 40th anniversary with a Symposium/Convention at Jekyll Island, Georgia April 12-14, 2013. The meeting will be held at the Oceanside Inn and Suites. The speaker lineup is fantastic and will offer something for everyone from basic histology, IHC, management, and specialties. There will also be a workshop for HT/HTL review. Come celebrate the "Wave of the Future" in Histology with GSH and our 40th anniversary in April. Reserve your room by March 1, 2013. Please see our link: http://www.histosearch.com/gsh/symposium Billie Zimmerman GSH secretary BZimmerm@gru.edu Any questions- please send them to me offline. Augusta State University and Georgia Health Sciences University have consolidated to become Georgia Regents University. Effective January 9, 2013, my email address has changed to BZIMMERM@gru.edu. Please update your address book to reflect this change. From mkent <@t> dermpathlab.com Tue Jan 22 11:10:07 2013 From: mkent <@t> dermpathlab.com (Michael Kent) Date: Tue Jan 22 11:11:57 2013 Subject: [Histonet] Treponema In-Reply-To: <1358810520.77614.YahooMailNeo@web124701.mail.ne1.yahoo.com> References: <1358810520.77614.YahooMailNeo@web124701.mail.ne1.yahoo.com> Message-ID: <27EB7FFB1A15F549B17F79B1A856C70BB45BFA@dlcs-sbs1.DPLCS.intra> Hello All, I am looking to bring Treponema pallidum antibody on board for FFPE in clinical derm. Would anyone recommend a source for control tissue for validation? Thanks, Mike Kent From LPaveli1 <@t> hurleymc.com Tue Jan 22 11:38:44 2013 From: LPaveli1 <@t> hurleymc.com (Lynette Pavelich) Date: Tue Jan 22 11:39:16 2013 Subject: [Histonet] RE: Treponema In-Reply-To: <27EB7FFB1A15F549B17F79B1A856C70BB45BFA@dlcs-sbs1.DPLCS.intra> References: <1358810520.77614.YahooMailNeo@web124701.mail.ne1.yahoo.com>, <27EB7FFB1A15F549B17F79B1A856C70BB45BFA@dlcs-sbs1.DPLCS.intra> Message-ID: <89F4666A496DC949A819ECC40E11C86701223053A3@EXCHANGEMB2.hmc.hurleymc.com> We purchase ours from HCS (Histology Control Systems). We use them for our IHC, and they work nicely. Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 ph: 810.262.9948 mobile: 810.444.7966 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Michael Kent [mkent@dermpathlab.com] Sent: Tuesday, January 22, 2013 12:10 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Treponema Hello All, I am looking to bring Treponema pallidum antibody on board for FFPE in clinical derm. Would anyone recommend a source for control tissue for validation? Thanks, Mike Kent _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Courtney.Pierce <@t> quintiles.com Tue Jan 22 12:04:35 2013 From: Courtney.Pierce <@t> quintiles.com (Courtney Pierce) Date: Tue Jan 22 12:04:41 2013 Subject: [Histonet] Question Message-ID: Are IHC high complexity test. Courtney Pierce IHC Specialist Quintiles Translational R&D - Oncology Innovation Navigating the new health 777 Oakmont Lane Suite 100 Westmont,IL 60559 Office: + 630-203-6234 courtney.pierce@quintiles.com clinical | commercial | consulting | capital ********************** IMPORTANT--PLEASE READ ************************ This electronic message, including its attachments, is COMPANY CONFIDENTIAL and may contain PROPRIETARY or LEGALLY PRIVILEGED information. If you are not the intended recipient, you are hereby notified that any use, disclosure, copying, or distribution of this message or any of the information included in it is unauthorized and strictly prohibited. If you have received this message in error, please immediately notify the sender by reply e-mail and permanently delete this message and its attachments, along with any copies thereof. Thank you. ************************************************************************ From BZIMMERM <@t> gru.edu Tue Jan 22 12:40:48 2013 From: BZIMMERM <@t> gru.edu (Zimmerman, Billie) Date: Tue Jan 22 12:40:53 2013 Subject: [Histonet] Georgia Histotechnology Society Message-ID: <7B3DEB32E69C034EACB479059C5DE3FF751CFB@EX-MLB-03.ad.georgiahealth.edu> I've had some email traffic about the link not working. By the way, this is a Georgia meeting but histotechs from other states are welcome to attend. It's a great way to earn continuing education while enjoying your surroundings! Please see link listed below: www.histosearch.com/gsh Augusta State University and Georgia Health Sciences University have consolidated to become Georgia Regents University. Effective January 9, 2013, my email address has changed to BZIMMERM@gru.edu. Please update your address book to reflect this change. From madeleinehuey <@t> gmail.com Tue Jan 22 12:41:12 2013 From: madeleinehuey <@t> gmail.com (Madeleine Huey) Date: Tue Jan 22 12:41:15 2013 Subject: [Histonet] TMA recipient block Message-ID: Hello, Anyone know the Vendor for TMA recipient blocks (3mm)? I saw a distributor carry them in the last CSH (Walnut Creek, CA). madeleine_h@elcaminohospital.org From NMargaryan <@t> luriechildrens.org Tue Jan 22 12:46:33 2013 From: NMargaryan <@t> luriechildrens.org (Margaryan, Naira) Date: Tue Jan 22 12:46:42 2013 Subject: [Histonet] H&E reagents Message-ID: <34B2EDA118548A4EB35D6E650345BA641F65F025@SV-EX08.childrensmemorial.org> What companies H&E reagents (catalog preferably) are the best for simple H&E staining? Thanks in advance, Naira From Lisa.White3 <@t> va.gov Tue Jan 22 12:46:46 2013 From: Lisa.White3 <@t> va.gov (White, Lisa M.) Date: Tue Jan 22 12:47:03 2013 Subject: [Histonet] Xylene Message-ID: <2B2ECF33934F5D4996D8BE03EFDF39760ACD26BB@VHAV09MSGA3.v09.med.va.gov> I know that Xylene is the number one choice for processing tissues. Some labs utilize Xylene Substitutes with good results. Looking for opinions on not only processing but thoughts on effects on staff. If you have articles please send them, would be very grateful. I have worked with two HT's in the past that have severe Xylene sensitivity. Trying to get my ducks in a row as management is considering a change for our lab. We currently use a sub and management is wanting to switch back to Xylene. Lisa White, HT(ASCP) Supervisory HT James H. Quillen VAMC PO Box 4000 Corner of Veterans Way and Lamont PLMS 113 Mountain Home, TN 37684 423-979-3567 423-979-3401 fax From RHONDA.GARLICK <@t> UCDENVER.EDU Tue Jan 22 14:08:14 2013 From: RHONDA.GARLICK <@t> UCDENVER.EDU (Garlick, Rhonda) Date: Tue Jan 22 14:08:17 2013 Subject: [Histonet] Message-ID: I've been doing some on-line searching with regards to the length of time tissues can remain in 4% Paraformaldehyde, but can't seem to get any definite answers. Can anyone help. Thanks, Rhonda Univ. of Denver Denver, CO From rjbuesa <@t> yahoo.com Tue Jan 22 14:17:52 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jan 22 14:17:56 2013 Subject: [Histonet] Question In-Reply-To: References: Message-ID: <1358885872.16210.YahooMailNeo@web163106.mail.bf1.yahoo.com> Yes! Ren? J. From: Courtney Pierce To: "histonet@lists.utsouthwestern.edu" Sent: Tuesday, January 22, 2013 1:04 PM Subject: [Histonet] Question Are IHC high complexity test. Courtney Pierce IHC Specialist Quintiles Translational R&D - Oncology Innovation Navigating the new health 777 Oakmont Lane Suite 100 Westmont,IL 60559 Office: + 630-203-6234 courtney.pierce@quintiles.com clinical | commercial | consulting | capital **********************? IMPORTANT--PLEASE READ? ************************ This electronic message, including its attachments, is COMPANY CONFIDENTIAL and may contain PROPRIETARY or LEGALLY PRIVILEGED information.? If you are not the intended recipient, you are hereby notified that any use, disclosure, copying, or distribution of this message or any of the information included in it is unauthorized and strictly prohibited.? If you have received this message in error, please immediately notify the sender by reply e-mail and permanently delete this message and its attachments, along with any copies thereof. Thank you. ************************************************************************ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Jan 22 14:19:10 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jan 22 14:19:13 2013 Subject: [Histonet] H&E reagents In-Reply-To: <34B2EDA118548A4EB35D6E650345BA641F65F025@SV-EX08.childrensmemorial.org> References: <34B2EDA118548A4EB35D6E650345BA641F65F025@SV-EX08.childrensmemorial.org> Message-ID: <1358885950.54312.YahooMailNeo@web163103.mail.bf1.yahoo.com> Richard Allan for me is the best. Ren? J. From: "Margaryan, Naira" To: "histonet@lists.utsouthwestern.edu" Sent: Tuesday, January 22, 2013 1:46 PM Subject: [Histonet] H&E reagents What companies H&E reagents (catalog preferably) are the best for simple H&E staining? Thanks in advance, Naira _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Jan 22 14:27:53 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jan 22 14:28:00 2013 Subject: [Histonet] Xylene In-Reply-To: <2B2ECF33934F5D4996D8BE03EFDF39760ACD26BB@VHAV09MSGA3.v09.med.va.gov> References: <2B2ECF33934F5D4996D8BE03EFDF39760ACD26BB@VHAV09MSGA3.v09.med.va.gov> Message-ID: <1358886473.9511.YahooMailNeo@web163106.mail.bf1.yahoo.com> Unfortunately you are right about xylene being preferentially used although at present the number of labs using it has been reduced to about 60%. You are also right that, compared with other "traditional" products, xylene produce results that can be considered as the "gold standard", but it is highly toxic. Due to that toxicity many chemical manufacturers started producing xylene substitutes?resulting in?25 D-Limonene derived and 35 alkane derivatives since the late 1970s, BUT neither can produce the same results as xylene. The only viable substitute producing even better results is a mixture of isopropyl alcohol and mineral oil. If you want to find more about these and related topics?on how to totally eliminate xylene from ALL the tasks in which it is involved now, please go to: http://www.histosearch.com/rene.html Ren? J. From: "White, Lisa M." To: histonet@lists.utsouthwestern.edu Sent: Tuesday, January 22, 2013 1:46 PM Subject: [Histonet] Xylene I know that Xylene is the number one choice for processing tissues. Some labs utilize Xylene Substitutes with good results.? Looking for opinions on not only processing but thoughts on effects on staff.? If you have articles please send them, would be very grateful.? I have worked with two HT's in the past that have severe Xylene sensitivity. Trying to get my ducks in a row as management is considering a change for our lab.? We currently use a sub and management is wanting to switch back to Xylene. Lisa White, HT(ASCP) Supervisory HT James H. Quillen VAMC PO Box 4000 Corner of Veterans Way and Lamont PLMS 113 Mountain Home, TN 37684 423-979-3567 423-979-3401 fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From marktarango <@t> gmail.com Tue Jan 22 15:02:09 2013 From: marktarango <@t> gmail.com (Mark Tarango) Date: Tue Jan 22 15:02:14 2013 Subject: [Histonet] Question In-Reply-To: References: Message-ID: The staining portion is not high complexity. The reading of the slide is. On Tue, Jan 22, 2013 at 10:04 AM, Courtney Pierce < Courtney.Pierce@quintiles.com> wrote: > Are IHC high complexity test. > > Courtney Pierce > IHC Specialist > Quintiles > Translational R&D - Oncology > Innovation > Navigating the new health > > 777 Oakmont Lane Suite 100 > Westmont,IL 60559 > > Office: + 630-203-6234 > courtney.pierce@quintiles.com > > clinical | commercial | consulting | capital > > > ********************** IMPORTANT--PLEASE READ ************************ > This electronic message, including its attachments, is COMPANY CONFIDENTIAL > and may contain PROPRIETARY or LEGALLY PRIVILEGED information. If you are > not the intended recipient, you are hereby notified that any use, > disclosure, > copying, or distribution of this message or any of the information included > in it is unauthorized and strictly prohibited. If you have received this > message in error, please immediately notify the sender by reply e-mail and > permanently delete this message and its attachments, along with any copies > thereof. Thank you. > ************************************************************************ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From b427297 <@t> aol.com Tue Jan 22 15:38:29 2013 From: b427297 <@t> aol.com (Jackie O'Connor) Date: Tue Jan 22 15:41:45 2013 Subject: [Histonet] H&E reagents In-Reply-To: <34B2EDA118548A4EB35D6E650345BA641F65F025@SV-EX08.childrensmemorial.org> References: <34B2EDA118548A4EB35D6E650345BA641F65F025@SV-EX08.childrensmemorial.org> Message-ID: <8CFC6FFF3F21F80-E74-2DCB0@webmail-m034.sysops.aol.com> I prefer the Richard Allen 7211 with alcoholic Eosin - consistent, dependable - we stain 50K slides a year. However, Leica has a similar product (Invented by the same guy) that will consistently stain 1500 slides before replacing. It works. You do need an extra reagent before the hematoxylin, tho. Ask your Leica rep. Good juice. Jackie O' -----Original Message----- From: Margaryan, Naira To: histonet Sent: Tue, Jan 22, 2013 12:46 pm Subject: [Histonet] H&E reagents What companies H&E reagents (catalog preferably) are the best for simple H&E taining? Thanks in advance, Naira _______________________________________________ istonet mailing list istonet@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet From Ceri.Allen <@t> nottingham.ac.uk Wed Jan 23 03:57:36 2013 From: Ceri.Allen <@t> nottingham.ac.uk (Ceri Allen) Date: Wed Jan 23 04:21:42 2013 Subject: [Histonet] Eosin intensity Message-ID: Dear All, Please can I ask what everyone uses as a standard Eosin recipe? The new pathologist in our department has asked for the stain to be darker, 'like the hospital does it'. I work on the research side of a vet school, so I'm not sure what histology departments in hospitals normally use. Many thanks Ceri Research Technician School of Veterinary Medicine and Science University of Nottingham This message and any attachment are intended solely for the addressee and may contain confidential information. If you have received this message in error, please send it back to me, and immediately delete it. Please do not use, copy or disclose the information contained in this message or in any attachment. Any views or opinions expressed by the author of this email do not necessarily reflect the views of the University of Nottingham. This message has been checked for viruses but the contents of an attachment may still contain software viruses which could damage your computer system: you are advised to perform your own checks. Email communications with the University of Nottingham may be monitored as permitted by UK legislation. From Papke.Louisa <@t> mayo.edu Wed Jan 23 10:10:40 2013 From: Papke.Louisa <@t> mayo.edu (Papke, Louisa M.) Date: Wed Jan 23 10:11:00 2013 Subject: [Histonet] P21 mouse brain Message-ID: <6A0C8E6B1A4F83458666DE85AD906F88029864@msgpexcha27b.mfad.mfroot.org> Has anyone worked with P21 mouse brain? Such as frozen or paraffin sectioning? Right now after perfusion and paraffin embedding, the brain literally falls apart when picking it up to embed. Any ideas or hints available would be welcome. Thank you in advanced, Louisa M Papke From mcauliff <@t> umdnj.edu Wed Jan 23 11:34:52 2013 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Wed Jan 23 11:32:39 2013 Subject: [Histonet] P21 mouse brain In-Reply-To: <6A0C8E6B1A4F83458666DE85AD906F88029864@msgpexcha27b.mfad.mfroot.org> References: <6A0C8E6B1A4F83458666DE85AD906F88029864@msgpexcha27b.mfad.mfroot.org> Message-ID: <51001F3C.70600@umdnj.edu> On 1/23/2013 11:10 AM, Papke, Louisa M. wrote: > Has anyone worked with P21 mouse brain? Such as frozen or paraffin sectioning? Right now after perfusion and paraffin embedding, the brain literally falls apart when picking it up to embed. Any ideas or hints available would be welcome. > > Thank you in advanced, > Louisa M Papke > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Perfusion with what? Fixation for how long? What is your processing schedule? Geoff -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From Papke.Louisa <@t> mayo.edu Wed Jan 23 11:54:57 2013 From: Papke.Louisa <@t> mayo.edu (Papke, Louisa M.) Date: Wed Jan 23 11:55:04 2013 Subject: [Histonet] P21 mouse brain In-Reply-To: <51001F3C.70600@umdnj.edu> References: <6A0C8E6B1A4F83458666DE85AD906F88029864@msgpexcha27b.mfad.mfroot.org> <51001F3C.70600@umdnj.edu> Message-ID: <6A0C8E6B1A4F83458666DE85AD906F8802989E@msgpexcha27b.mfad.mfroot.org> The mice were perfused with 4% PFA. Then left in 4% PFA for about 2 weeks. The processing schedule is as follows: 70% Alcohol 15 min. 80% Alcohol 15 min. 95% Alcohol 15 min. 95% Alcohol 15 min. 100% Alcohol 15 min. 100% Alcohol 15 min. 100% Alcohol 20 min. Xylene 20 min Xylene 20 min Xylene 45 min Paraffin 30 min Paraffin 40 min. temp 58 degrees. No vac. on any of the stations. This is the same program I use on mice spinal cords. Half the mice are under hypoxic conditions and the other half normoxic conditions. The normoxic brains also fell apart. I also did another group with the perfusion with 4% PFA then cryoprotected with 30% Sucrose. They didn't cut well. Wrinkles and air bubbles. I will try cutting at a -25 instead of -20. Also, the sections fell off as soon as I put them in water. I will admit that I have only worked with adult mouse brain and spinal cord. So this is a new area for me. Thank you again for suggestions and advice. Louisa M. Papke Research Technologist Demyelinating Diseases Research -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Geoff McAuliffe Sent: Wednesday, January 23, 2013 11:35 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] P21 mouse brain On 1/23/2013 11:10 AM, Papke, Louisa M. wrote: > Has anyone worked with P21 mouse brain? Such as frozen or paraffin sectioning? Right now after perfusion and paraffin embedding, the brain literally falls apart when picking it up to embed. Any ideas or hints available would be welcome. > > Thank you in advanced, > Louisa M Papke > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Perfusion with what? Fixation for how long? What is your processing schedule? Geoff -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jim000001 <@t> hotmail.com Wed Jan 23 12:03:58 2013 From: jim000001 <@t> hotmail.com (Jim Jones) Date: Wed Jan 23 12:04:02 2013 Subject: [Histonet] TMA recipient block In-Reply-To: References: Message-ID: We are using a 3mm TMA block. Purchased it from Arraymold. www.arraymold.com Jimmy > Date: Tue, 22 Jan 2013 10:41:12 -0800 > From: madeleinehuey@gmail.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] TMA recipient block > > Hello, > Anyone know the Vendor for TMA recipient blocks (3mm)? I saw a > distributor carry them in the last CSH (Walnut Creek, CA). > madeleine_h@elcaminohospital.org > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Dorothy.L.Webb <@t> HealthPartners.Com Wed Jan 23 12:13:49 2013 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Wed Jan 23 12:14:01 2013 Subject: [Histonet] CLIA Message-ID: <65365F35C0F2EF4D846EC3CA73E49C430227AFFBCC4D@HPEMX3.HealthPartners.int> According to CLIA and CAP, IHC is considered High Complexity testing which is why some labs only allow a degreed tech to perform IHC tests. ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 From Donna.Willis <@t> baylorhealth.edu Wed Jan 23 12:20:05 2013 From: Donna.Willis <@t> baylorhealth.edu (Willis, Donna G.) Date: Wed Jan 23 12:20:54 2013 Subject: [Histonet] Tissue Microarray Message-ID: <2572B4D63B62E64A8078D8BBE34D4078018EC3@BHDAMAEXML2.bhcs.pvt> I need to purchase a Tissue Microarray instrument. Would folks please give me their preferences and why. Thanks, Donna Willis, HT/HTL (ASCP) Anatomic Pathology Manager Baylor University Medical Center-Dallas ph. 214-820-2465 office ph. 214-725-6184 mobile donna.willis@baylorhealth.edu ********************************************************************** This e-mail may contain confidential and/or privileged information. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. From Caroline.Pratt <@t> uphs.upenn.edu Wed Jan 23 12:22:36 2013 From: Caroline.Pratt <@t> uphs.upenn.edu (Pratt, Caroline) Date: Wed Jan 23 12:22:42 2013 Subject: [Histonet] CLIA In-Reply-To: <65365F35C0F2EF4D846EC3CA73E49C430227AFFBCC4D@HPEMX3.HealthPartners.int> References: <65365F35C0F2EF4D846EC3CA73E49C430227AFFBCC4D@HPEMX3.HealthPartners.int> Message-ID: That's our policy at Penn Medicine as well. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Wednesday, January 23, 2013 1:14 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] CLIA According to CLIA and CAP, IHC is considered High Complexity testing which is why some labs only allow a degreed tech to perform IHC tests. ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From TNMayer <@t> mdanderson.org Wed Jan 23 12:54:31 2013 From: TNMayer <@t> mdanderson.org (Mayer,Toysha N) Date: Wed Jan 23 12:56:27 2013 Subject: [Histonet] RE: Histonet Digest, Vol 110, Issue 27 In-Reply-To: References: Message-ID: <47E9B2C01DDDD94881EACD2DC44EBC8802F41B@D1PWPEXMBX05.mdanderson.edu> Try contacting Pauline Grennan at Texas Children's Hospital Neuropathology Lab, 832-824-1000 is the main hospital number. She has a number of specialized procedures for all types of neuro tissue. Toysha N. Mayer, MBA, HT (ASCP) Instructor, Education Coordinator Program in Histotechnology School of Health Professions MD Anderson Cancer Center (713) 563-3481 tnmayer@mdanderson.org Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Mon, 21 Jan 2013 11:58:33 -0600 From: "Webb, Dorothy L" Subject: [Histonet] brain tissue To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <65365F35C0F2EF4D846EC3CA73E49C430227AFFBCC2F@HPEMX3.HealthPartners.int> Content-Type: text/plain; charset="us-ascii" How is everyone processing brain tissue from brain surgery, not necessarily a small bx? Does anyone have a separately timed process for such tissue to get them out faster? And what is the minimum fixation time suggested? Thanks, Dorothy Webb From lcolbert <@t> pathmdlabs.com Wed Jan 23 12:55:46 2013 From: lcolbert <@t> pathmdlabs.com (Laurie Colbert) Date: Wed Jan 23 13:01:19 2013 Subject: [Histonet] Recycled Xylene on Tissue Processor Message-ID: <12ECD7346266D74691EC2BFC75285E4501198EA1@BFL323E10.pathmdlabs.local> Has anyone that uses recycled xylene on the tissue processor ever noticed that it dries out the biopsies (specifically GI bx's)?? We are having issues with the GI's being dried out, and I'm wondering if this may be the cause. Laurie Colbert From thigginsht <@t> msn.com Wed Jan 23 13:44:19 2013 From: thigginsht <@t> msn.com (Tim Higgins) Date: Wed Jan 23 13:44:29 2013 Subject: [Histonet] Re: Question Message-ID: The professional interpretation is considered a high complexity test but not the actual technical component. Thanks, Timothy N. Higgins, HT (ASCP), QIHC From hfedor <@t> jhmi.edu Wed Jan 23 14:03:57 2013 From: hfedor <@t> jhmi.edu (Helen Fedor) Date: Wed Jan 23 14:04:03 2013 Subject: [Histonet] RE: Tissue Microarray In-Reply-To: <2572B4D63B62E64A8078D8BBE34D4078018EC3@BHDAMAEXML2.bhcs.pvt> References: <2572B4D63B62E64A8078D8BBE34D4078018EC3@BHDAMAEXML2.bhcs.pvt> Message-ID: Hello Donna, We have two different instruments, and they each have value. But if you are only interested in making small arrays for control blocks then you wouldn't need to spend the amount of money that the larger Arrays would cost. http://www.pathologydevices.com/TMArrayer.htm this company sells both kinds and also sells the needles. The former company Beecher is now in Estonia under http://www.estigen.com/ I would be happy to speak to you about other small models if you are interested or would like to discuss these instruments. Helen L. Fedor Prostate Tissue Bank, Manager Oncology Tissue Services, Manager Johns Hopkins University 600 N. Wolfe St,?| Marburg Room 406 Baltimore, MD?| 21287-7065 410.614.1660 http://tmalab.jhmi.edu/ http://prostatebiorepository.org/ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Willis, Donna G. Sent: Wednesday, January 23, 2013 1:20 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tissue Microarray I need to purchase a Tissue Microarray instrument. Would folks please give me their preferences and why. Thanks, Donna Willis, HT/HTL (ASCP) Anatomic Pathology Manager Baylor University Medical Center-Dallas ph. 214-820-2465 office ph. 214-725-6184 mobile donna.willis@baylorhealth.edu ********************************************************************** This e-mail may contain confidential and/or privileged information. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kmilne <@t> bccancer.bc.ca Wed Jan 23 14:22:38 2013 From: kmilne <@t> bccancer.bc.ca (Milne, Katy) Date: Wed Jan 23 14:22:50 2013 Subject: [Histonet] Custom ISH probes In-Reply-To: <18f7bd90-1523-41da-ab5f-6f28e1108f9b@SRVEXHT02.phsabc.ehcnet.ca> References: <18f7bd90-1523-41da-ab5f-6f28e1108f9b@SRVEXHT02.phsabc.ehcnet.ca> Message-ID: <3FEFF18FF4E1914A9AB7D8498591BE8601237022296A@VEXCCR02.phsabc.ehcnet.ca> Hi all, Our research lab is starting to get into ISH and is looking for a supplier of DIG-labelled ISH probes for projects where making our own isn't a desired option. Does anyone have suggestions for reliable, economical companies that are easy to deal with and provide quality DNA oligo probes for use on FFPE mouse and human tissue? We're up in Canada so we'd have to get it across the border without insane shipping costs/hassles too. Mainly we'd be looking at cytokines, targets for which there are no Abs, that sort of thing. Thanks, Katy Milne Trev and Joyce Deeley Research Centre From rjbuesa <@t> yahoo.com Wed Jan 23 14:47:04 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jan 23 14:47:08 2013 Subject: [Histonet] Recycled Xylene on Tissue Processor In-Reply-To: <12ECD7346266D74691EC2BFC75285E4501198EA1@BFL323E10.pathmdlabs.local> References: <12ECD7346266D74691EC2BFC75285E4501198EA1@BFL323E10.pathmdlabs.local> Message-ID: <1358974024.35231.YahooMailNeo@web163104.mail.bf1.yahoo.com> If xylene is recycled properly and has no alcohol residues, i should not pose any problems. It will "clear" just like the original "pure" xylene, at least that is what I found with my recycled xylene for more than 10 years. The problem you describe should have a different cause. Ren? J. From: Laurie Colbert To: "Histonet Post (histonet@lists.utsouthwestern.edu)" Sent: Wednesday, January 23, 2013 1:55 PM Subject: [Histonet] Recycled Xylene on Tissue Processor Has anyone that uses recycled xylene on the tissue processor ever noticed that it dries out the biopsies (specifically GI bx's)??? We are having issues with the GI's being dried out, and I'm wondering if this may be the cause. Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ttruscot <@t> vetmed.wsu.edu Wed Jan 23 15:00:56 2013 From: ttruscot <@t> vetmed.wsu.edu (Truscott, Tom) Date: Wed Jan 23 15:01:04 2013 Subject: [Histonet] Recycled Xylene on Tissue Processor In-Reply-To: <1358974024.35231.YahooMailNeo@web163104.mail.bf1.yahoo.com> References: <12ECD7346266D74691EC2BFC75285E4501198EA1@BFL323E10.pathmdlabs.local> <1358974024.35231.YahooMailNeo@web163104.mail.bf1.yahoo.com> Message-ID: <9EF5279EBDFE6E4FB6605E8F183A002743D4C83A@CVM76.vetmed.wsu.edu> If the xylene was not recycled properly and retained too much alcohol, then the extra alcohol could be the culprit and do the "drying'. Tom T -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, January 23, 2013 12:47 PM To: Laurie Colbert; Histonet Post (histonet@lists.utsouthwestern.edu) Subject: Re: [Histonet] Recycled Xylene on Tissue Processor If xylene is recycled properly and has no alcohol residues, i should not pose any problems. It will "clear" just like the original "pure" xylene, at least that is what I found with my recycled xylene for more than 10 years. The problem you describe should have a different cause. Ren? J. From: Laurie Colbert To: "Histonet Post (histonet@lists.utsouthwestern.edu)" Sent: Wednesday, January 23, 2013 1:55 PM Subject: [Histonet] Recycled Xylene on Tissue Processor Has anyone that uses recycled xylene on the tissue processor ever noticed that it dries out the biopsies (specifically GI bx's)??? We are having issues with the GI's being dried out, and I'm wondering if this may be the cause. Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mcauliff <@t> umdnj.edu Wed Jan 23 15:10:30 2013 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Wed Jan 23 15:08:17 2013 Subject: [Histonet] P21 mouse brain In-Reply-To: <6A0C8E6B1A4F83458666DE85AD906F8802989E@msgpexcha27b.mfad.mfroot.org> References: <6A0C8E6B1A4F83458666DE85AD906F88029864@msgpexcha27b.mfad.mfroot.org> <51001F3C.70600@umdnj.edu> <6A0C8E6B1A4F83458666DE85AD906F8802989E@msgpexcha27b.mfad.mfroot.org> Message-ID: <510051C6.40800@umdnj.edu> I think your processing schedule is too short for a whole mouse brain. Do you really need to cut the entire brain in one block? I suggest cutting the brain in half down the midline or in thirds rostral-caudal and doubling or tripling the times in all steps. Also add a 100% alc:xylene 1:1 mix step. A third paraffin is also a good idea, especially since you don't have vacuum. As to the problems with cryo sections, first wash the brain in buffer, then try 10% sucrose cold, then 25-30% cold over night. How are you freezing the brains? Just putting them in the cryostat is not sufficient, they must be completely frozen very quickly, then allowed to equilibrate in the cryostat. -20C should be fine, is your knife sharp? Geoff On 1/23/2013 12:54 PM, Papke, Louisa M. wrote: > The mice were perfused with 4% PFA. Then left in 4% PFA for about 2 weeks. > The processing schedule is as follows: > 70% Alcohol 15 min. > 80% Alcohol 15 min. > 95% Alcohol 15 min. > 95% Alcohol 15 min. > 100% Alcohol 15 min. > 100% Alcohol 15 min. > 100% Alcohol 20 min. > Xylene 20 min > Xylene 20 min > Xylene 45 min > Paraffin 30 min > Paraffin 40 min. temp 58 degrees. No vac. on any of the stations. > > This is the same program I use on mice spinal cords. > Half the mice are under hypoxic conditions and the other half normoxic conditions. The normoxic brains also fell apart. > I also did another group with the perfusion with 4% PFA then cryoprotected with 30% Sucrose. They didn't cut well. Wrinkles and air bubbles. I will try cutting at a -25 instead of -20. Also, the sections fell off as soon as I put them in water. > > I will admit that I have only worked with adult mouse brain and spinal cord. So this is a new area for me. > > Thank you again for suggestions and advice. > Louisa M. Papke > Research Technologist > Demyelinating Diseases Research > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Geoff McAuliffe > Sent: Wednesday, January 23, 2013 11:35 AM > To: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] P21 mouse brain > > On 1/23/2013 11:10 AM, Papke, Louisa M. wrote: >> Has anyone worked with P21 mouse brain? Such as frozen or paraffin sectioning? Right now after perfusion and paraffin embedding, the brain literally falls apart when picking it up to embed. Any ideas or hints available would be welcome. >> >> Thank you in advanced, >> Louisa M Papke >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> > Perfusion with what? Fixation for how long? What is your processing schedule? > > Geoff > > -- > -- > ********************************************** > Geoff McAuliffe, Ph.D. > Neuroscience and Cell Biology > Robert Wood Johnson Medical School > 675 Hoes Lane, Piscataway, NJ 08854 > voice: (732)-235-4583 > mcauliff@umdnj.edu > ********************************************** > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From marktarango <@t> gmail.com Wed Jan 23 15:20:27 2013 From: marktarango <@t> gmail.com (Mark Tarango) Date: Wed Jan 23 15:20:33 2013 Subject: [Histonet] Re: Question In-Reply-To: References: Message-ID: If anyone has any documentation that says the staining of IHC slides is NOT high complexity it would help a histonetter out there. I got an e-mail from someone who is HT(ASCP)QIHC but does not have an AA degree. Their lab director is threatening their job saying they aren't qualified to do IHC staining. If anyone has something to refer to it would be helpful for this person. I already suggested contacting CAP and getting a written response. I believe IHC is high complexity but not the staining portion. Since no result is being produced by the IHC tech how can this be high complexity? thanks Mark On Wed, Jan 23, 2013 at 11:44 AM, Tim Higgins wrote: > > The professional interpretation is considered a high complexity test but > not the actual technical component. > > > > Thanks, > > > > Timothy N. Higgins, HT (ASCP), QIHC > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From brannon <@t> alliedsearchpartners.com Wed Jan 23 15:35:15 2013 From: brannon <@t> alliedsearchpartners.com (Brannon Owens) Date: Wed Jan 23 15:35:24 2013 Subject: [Histonet] Pathology Manager opening in Grand Rapids, MI Message-ID: Position Title: Pathology Manager Location: Grand Rapids, MI area Schedule: Full Time/Permanent Send an email to Brannon@alliedsearchpartners.com for a full job description or to submit a resume for consideration. Brannon Owens Recruitment Manager Allied Search Partners From tony.henwood <@t> health.nsw.gov.au Wed Jan 23 15:38:24 2013 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Wed Jan 23 15:38:36 2013 Subject: [Histonet] RE: Eosin intensity In-Reply-To: References: Message-ID: <6D6BD1DE8A5571489398B392A38A71579D2249EB@xmdb04.nch.kids> Ceri, There are several ways to darken the eosin counterstain: 1. Stain longer in eosin, though with some eosins including eosin Y, this might not necessarily darken the staining since the eosin Y is a yellow red, eosin B seems to be more bluish red 2. Under-differentiate the haematoxylin, though this can result in overly stained nuclei (especially those of plasma cells). 3. Include a darker red dye in your eosin stain eg Erythrosine or even phloxine (not quite as dark). Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ceri Allen Sent: Wednesday, 23 January 2013 8:58 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Eosin intensity Dear All, Please can I ask what everyone uses as a standard Eosin recipe? The new pathologist in our department has asked for the stain to be darker, 'like the hospital does it'. I work on the research side of a vet school, so I'm not sure what histology departments in hospitals normally use. Many thanks Ceri Research Technician School of Veterinary Medicine and Science University of Nottingham This message and any attachment are intended solely for the addressee and may contain confidential information. If you have received this message in error, please send it back to me, and immediately delete it. Please do not use, copy or disclose the information contained in this message or in any attachment. Any views or opinions expressed by the author of this email do not necessarily reflect the views of the University of Nottingham. This message has been checked for viruses but the contents of an attachment may still contain software viruses which could damage your computer system: you are advised to perform your own checks. Email communications with the University of Nottingham may be monitored as permitted by UK legislation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From tony.henwood <@t> health.nsw.gov.au Wed Jan 23 16:48:07 2013 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Wed Jan 23 16:48:24 2013 Subject: [Histonet] RE: Recycled Xylene on Tissue Processor In-Reply-To: <12ECD7346266D74691EC2BFC75285E4501198EA1@BFL323E10.pathmdlabs.local> References: <12ECD7346266D74691EC2BFC75285E4501198EA1@BFL323E10.pathmdlabs.local> Message-ID: <6D6BD1DE8A5571489398B392A38A71579D226A37@xmdb04.nch.kids> Hi Laurie, We use recycled xylene and found that infrequently we may produce a poor quality xylene (often contaminated with xylene). This will affect tissue processing. We regularly QC our recycled xylene using the CBG Biotech technique: Testing Procedure 1. To a clean, dry 100 ml mixing cylinder graduate, add sufficient recovered xylene so that the bottom of the meniscus is aligned with the top edge of the 85 ml mark on the graduate. 2. Add water to the graduate until the bottom of the meniscus aligns with the top edge of the 100 ml mark on the graduate. At this point, 15 ml of water will have been added to 85 ml of recovered xylene. 3. Stopper the graduate and invert the mixture. Allow the mixture to settle, making sure that all of the water settles to the bottom of the graduate. No water should remain clinging to the sides of the graduate above the xylene/water separation point. This separation point should be near the 15 ml level of the graduate. (Note: xylene floats on top of the water). 4. Carefully inspect and record the point of separation between the water and xylene using the bottom of the meniscus as the separation point. 5. Subtract 15 ml from the quantity of water indicated in step 5. The remainder plus an additional 0.1 correction factor equals the percentage of recovered xylene impurities. EXAMPLE: Xylene/Water separation point is indicated to be 15.5 ml. (15.5 - 15) + 0.1 = 0.6% impurities. Therefore, the recovered xylene is 99.4% pure. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Thursday, 24 January 2013 5:56 AM To: Histonet Post (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Recycled Xylene on Tissue Processor Has anyone that uses recycled xylene on the tissue processor ever noticed that it dries out the biopsies (specifically GI bx's)?? We are having issues with the GI's being dried out, and I'm wondering if this may be the cause. Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From mgiorgi <@t> icplab.com Wed Jan 23 17:25:09 2013 From: mgiorgi <@t> icplab.com (Miranda Giorgi) Date: Wed Jan 23 17:25:15 2013 Subject: [Histonet] Open Graveyard Histotech Position in Spokane, WA Message-ID: <46BB82E6FC36E44FB454E00C82DBDC56CCB30A@icpmail.PAI.E-PATHOLOGY.com> Hello All, Please see below the current job posting for a Histotechnologist position at InCyte Pathology in Spokane, WA. Only interested candidates should reply. Please do not respond if you are from a recruitment service. Thank you! Histology Analyst, Graveyard Shift (M-F) InCyte Pathology is a full-service, pathologist-owned anatomic pathology laboratory. Our one-hundred employees and twenty-one board-certified pathologists provide exceptional laboratory services to hospitals and physician offices throughout the Pacific Northwest, Montana, and Alaska from our state-of-the-art facility located in Spokane Valley, Washington. We are just a short drive from five major ski resorts, several beautiful lakes, and a variety of outdoor activities. Qualifications: HT certified or HTL registry eligible, and two years of laboratory experience. This position performs all routine procedures in orienting, sectioning, and staining tissue specimens to produce slides which will aide pathologists in diagnosis. Preference will be given to those candidates who are IHC qualified and have proven leadership qualities. We offer competitive salaries and an outstanding benefits package including medical, dental, life and disability insurance, 401(k), and a liberal paid time off program. Relocation assistance is available. Please email your cover letter and resume to humanresources@incytepathology.com. Please reference where you saw this ad. No phone calls or solicitors! Miranda Giorgi, HTL (ASCP)CM Histology Supervisor InCyte Pathology This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the InCyte Privacy Officer at privacy@icplab.com or call (509) 892-2700. From BSullivan <@t> virtua.org Thu Jan 24 06:57:04 2013 From: BSullivan <@t> virtua.org (Sullivan, Beatrice) Date: Thu Jan 24 06:57:12 2013 Subject: [Histonet] Re: Question In-Reply-To: References: Message-ID: <6932520047F7EE46B512E9801344F160038CC5@ExchangeMB-1.Virtua.org> I called CAP a short time ago about who was qualified to run these. I was told then that there was no requirement for a degreed person to run these. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Tarango Sent: Wednesday, January 23, 2013 4:20 PM To: Tim Higgins Cc: histonet@lists.utsouthwestern.edu; courtney.pierce@quintiles.com Subject: Re: [Histonet] Re: Question If anyone has any documentation that says the staining of IHC slides is NOT high complexity it would help a histonetter out there. I got an e-mail from someone who is HT(ASCP)QIHC but does not have an AA degree. Their lab director is threatening their job saying they aren't qualified to do IHC staining. If anyone has something to refer to it would be helpful for this person. I already suggested contacting CAP and getting a written response. I believe IHC is high complexity but not the staining portion. Since no result is being produced by the IHC tech how can this be high complexity? thanks Mark On Wed, Jan 23, 2013 at 11:44 AM, Tim Higgins wrote: > > The professional interpretation is considered a high complexity test > but not the actual technical component. > > > > Thanks, > > > > Timothy N. Higgins, HT (ASCP), QIHC > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message, and any included attachments, are from Virtua Health or its related affiliates and is intended only for the addressee(s). The information contained herein is privileged, proprietary or may include confidential information and/or protected patient health information. Any unauthorized review, forwarding, printing, copying, distributing, or otherwise disseminating or taking any action based on such information is strictly prohibited. If you have received this message in error, or have reason to believe you are not authorized to receive it, please delete this message promptly and notify the sender by e-mail with a copy to ISSECURITY@virtua.org. Thank you From histotech <@t> imagesbyhopper.com Thu Jan 24 10:18:36 2013 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Thu Jan 24 10:18:54 2013 Subject: [Histonet] Re: Question In-Reply-To: <6932520047F7EE46B512E9801344F160038CC5@ExchangeMB-1.Virtua.org> References: <6932520047F7EE46B512E9801344F160038CC5@ExchangeMB-1.Virtua.org> Message-ID: <000301cdfa4e$79577420$6c065c60$@imagesbyhopper.com> It also depends on the State you are in. Florida considers this a high complexity test and requires that a licensed technologist either do the test or oversee a licensed technician who is performing the test. >From my view, especially with all the automatic stainers out there, simply putting the slides on/off the stainer should not be considered high complexity! The interpretation of the stain should be done by a trained individual though. Just my $0.02. Michelle -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sullivan, Beatrice Sent: Thursday, January 24, 2013 7:57 AM To: Mark Tarango; Tim Higgins Cc: histonet@lists.utsouthwestern.edu; courtney.pierce@quintiles.com Subject: RE: [Histonet] Re: Question I called CAP a short time ago about who was qualified to run these. I was told then that there was no requirement for a degreed person to run these. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Tarango Sent: Wednesday, January 23, 2013 4:20 PM To: Tim Higgins Cc: histonet@lists.utsouthwestern.edu; courtney.pierce@quintiles.com Subject: Re: [Histonet] Re: Question If anyone has any documentation that says the staining of IHC slides is NOT high complexity it would help a histonetter out there. I got an e-mail from someone who is HT(ASCP)QIHC but does not have an AA degree. Their lab director is threatening their job saying they aren't qualified to do IHC staining. If anyone has something to refer to it would be helpful for this person. I already suggested contacting CAP and getting a written response. I believe IHC is high complexity but not the staining portion. Since no result is being produced by the IHC tech how can this be high complexity? thanks Mark On Wed, Jan 23, 2013 at 11:44 AM, Tim Higgins wrote: > > The professional interpretation is considered a high complexity test > but not the actual technical component. > > > > Thanks, > > > > Timothy N. Higgins, HT (ASCP), QIHC > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message, and any included attachments, are from Virtua Health or its related affiliates and is intended only for the addressee(s). The information contained herein is privileged, proprietary or may include confidential information and/or protected patient health information. Any unauthorized review, forwarding, printing, copying, distributing, or otherwise disseminating or taking any action based on such information is strictly prohibited. If you have received this message in error, or have reason to believe you are not authorized to receive it, please delete this message promptly and notify the sender by e-mail with a copy to ISSECURITY@virtua.org. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----- No virus found in this message. Checked by AVG - www.avg.com Version: 2013.0.2890 / Virus Database: 2639/6052 - Release Date: 01/23/13 ----- No virus found in this message. Checked by AVG - www.avg.com Version: 2013.0.2890 / Virus Database: 2639/6054 - Release Date: 01/24/13 From rjbuesa <@t> yahoo.com Thu Jan 24 11:42:35 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jan 24 11:42:42 2013 Subject: [Histonet] Re: Question In-Reply-To: <000301cdfa4e$79577420$6c065c60$@imagesbyhopper.com> References: <6932520047F7EE46B512E9801344F160038CC5@ExchangeMB-1.Virtua.org> <000301cdfa4e$79577420$6c065c60$@imagesbyhopper.com> Message-ID: <1359049355.10322.YahooMailNeo@web163102.mail.bf1.yahoo.com> We have to remember that it is not just putting slides in/out of automated stainer. It is also understanding what the histotech is doing, how to design a validation experiment, how to look for an antibody the pathologists wants to test, how to troubleshoot a problem, how to justify switching from a detection system to another more convenient to the procedure on hand that for any reason no longer "works". A histotech needs to know what s/he is doing. And to do all of the above has to have knowledge besides the training and?the mechanics of what s/he is doing. Designating a task as "high complexity" demands that the histotech has the basic theoretical knowledge and therefore deserves a better pay. As some people has said before, putting slides in/out of an automated stainer of any kind) can be done by a monkey. Understanding what is involved in IHC is above a monkey's pay grade. Ren? J. From: "histotech@imagesbyhopper.com" To: "'Sullivan, Beatrice'" ; 'Mark Tarango' ; 'Tim Higgins' Cc: histonet@lists.utsouthwestern.edu; courtney.pierce@quintiles.com Sent: Thursday, January 24, 2013 11:18 AM Subject: RE: [Histonet] Re: Question It also depends on the State you are in.? Florida considers this a high complexity test and requires that a licensed technologist either do the test or oversee a licensed technician who is performing the test. >>From my view, especially with all the automatic stainers out there, simply putting the slides on/off the stainer should not be considered high complexity!? The interpretation of the stain should be done by a trained individual though. Just my $0.02. Michelle -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sullivan, Beatrice Sent: Thursday, January 24, 2013 7:57 AM To: Mark Tarango; Tim Higgins Cc: histonet@lists.utsouthwestern.edu; courtney.pierce@quintiles.com Subject: RE: [Histonet] Re: Question I called CAP a short time ago about who was qualified to run these. I was told then that there was no requirement for a degreed person to run these. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Tarango Sent: Wednesday, January 23, 2013 4:20 PM To: Tim Higgins Cc: histonet@lists.utsouthwestern.edu; courtney.pierce@quintiles.com Subject: Re: [Histonet] Re: Question If anyone has any documentation that says the staining of IHC slides is NOT high complexity it would help a histonetter out there.? I got an e-mail from someone who is HT(ASCP)QIHC but does not have an AA degree.? Their lab director is threatening their job saying they aren't qualified to do IHC staining.? If anyone has something to refer to it would be helpful for this person.? I already suggested contacting CAP and getting a written response. I believe IHC is high complexity but not the staining portion.? Since no result is being produced by the IHC tech how can this be high complexity? thanks Mark On Wed, Jan 23, 2013 at 11:44 AM, Tim Higgins wrote: > > The professional interpretation is considered a high complexity test > but not the actual technical component. > > > > Thanks, > > > > Timothy N. Higgins, HT (ASCP), QIHC > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message, and any included attachments, are from Virtua Health or its related affiliates and is intended only for the addressee(s). The information contained herein is privileged, proprietary or may include confidential information and/or protected patient health information. Any unauthorized review, forwarding, printing, copying, distributing, or otherwise disseminating or taking any action based on such information is strictly prohibited. If you have received this message in error, or have reason to believe you are not authorized to receive it, please delete this message promptly and notify the sender by e-mail with a copy to ISSECURITY@virtua.org. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----- No virus found in this message. Checked by AVG - http://www.avg.com/ Version: 2013.0.2890 / Virus Database: 2639/6052 - Release Date: 01/23/13 ----- No virus found in this message. Checked by AVG - www.avg.com Version: 2013.0.2890 / Virus Database: 2639/6054 - Release Date: 01/24/13 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Jan 24 11:44:00 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jan 24 11:44:09 2013 Subject: Fw: [Histonet] Re: Question In-Reply-To: <1359049355.10322.YahooMailNeo@web163102.mail.bf1.yahoo.com> References: <6932520047F7EE46B512E9801344F160038CC5@ExchangeMB-1.Virtua.org> <000301cdfa4e$79577420$6c065c60$@imagesbyhopper.com> <1359049355.10322.YahooMailNeo@web163102.mail.bf1.yahoo.com> Message-ID: <1359049440.20318.YahooMailNeo@web163102.mail.bf1.yahoo.com> We have to remember that it is not just putting slides in/out of automated stainer. It is also understanding what the histotech is doing, how to design a validation experiment, how to look for an antibody the pathologists wants to test, how to troubleshoot a problem, how to justify switching from a detection system to another more convenient to the procedure on hand that for any reason no longer "works". A histotech needs to know what s/he is doing. And to do all of the above has to have knowledge besides the training and?the mechanics of what s/he is doing. Designating a task as "high complexity" demands that the histotech has the basic theoretical knowledge and therefore deserves a better pay. As some people has said before, putting slides in/out of an automated stainer of any kind) can be done by a monkey. Understanding what is involved in IHC is above a monkey's pay grade. Ren? J. From: "histotech@imagesbyhopper.com" To: "'Sullivan, Beatrice'" ; 'Mark Tarango' ; 'Tim Higgins' Cc: histonet@lists.utsouthwestern.edu; courtney.pierce@quintiles.com Sent: Thursday, January 24, 2013 11:18 AM Subject: RE: [Histonet] Re: Question It also depends on the State you are in.? Florida considers this a high complexity test and requires that a licensed technologist either do the test or oversee a licensed technician who is performing the test. >>From my view, especially with all the automatic stainers out there, simply putting the slides on/off the stainer should not be considered high complexity!? The interpretation of the stain should be done by a trained individual though. Just my $0.02. Michelle -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sullivan, Beatrice Sent: Thursday, January 24, 2013 7:57 AM To: Mark Tarango; Tim Higgins Cc: histonet@lists.utsouthwestern.edu; courtney.pierce@quintiles.com Subject: RE: [Histonet] Re: Question I called CAP a short time ago about who was qualified to run these. I was told then that there was no requirement for a degreed person to run these. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Tarango Sent: Wednesday, January 23, 2013 4:20 PM To: Tim Higgins Cc: histonet@lists.utsouthwestern.edu; courtney.pierce@quintiles.com Subject: Re: [Histonet] Re: Question If anyone has any documentation that says the staining of IHC slides is NOT high complexity it would help a histonetter out there.? I got an e-mail from someone who is HT(ASCP)QIHC but does not have an AA degree.? Their lab director is threatening their job saying they aren't qualified to do IHC staining.? If anyone has something to refer to it would be helpful for this person.? I already suggested contacting CAP and getting a written response. I believe IHC is high complexity but not the staining portion.? Since no result is being produced by the IHC tech how can this be high complexity? thanks Mark On Wed, Jan 23, 2013 at 11:44 AM, Tim Higgins wrote: > > The professional interpretation is considered a high complexity test > but not the actual technical component. > > > > Thanks, > > > > Timothy N. Higgins, HT (ASCP), QIHC > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message, and any included attachments, are from Virtua Health or its related affiliates and is intended only for the addressee(s). The information contained herein is privileged, proprietary or may include confidential information and/or protected patient health information. Any unauthorized review, forwarding, printing, copying, distributing, or otherwise disseminating or taking any action based on such information is strictly prohibited. If you have received this message in error, or have reason to believe you are not authorized to receive it, please delete this message promptly and notify the sender by e-mail with a copy to ISSECURITY@virtua.org. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----- No virus found in this message. Checked by AVG - http://www.avg.com/ Version: 2013.0.2890 / Virus Database: 2639/6052 - Release Date: 01/23/13 ----- No virus found in this message. Checked by AVG - www.avg.com Version: 2013.0.2890 / Virus Database: 2639/6054 - Release Date: 01/24/13 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From theresec <@t> slonepartners.com Thu Jan 24 13:17:12 2013 From: theresec <@t> slonepartners.com (Therese Cook) Date: Thu Jan 24 13:17:21 2013 Subject: [Histonet] Re: Histonet Digest, Vol 110, Issue 31 Message-ID: <9F5E8E1A-B9F7-411D-AED6-2D3567329853@slonepartners.com> Below is information on a great position in Western NY. Please contact me if interested in learning more... Slone Partners seeks a Manager of Anatomic Pathology for a health system in Western New York. The Manager will be responsible for the day to day operations of the department, consisting of 40 FTEs in histology, cytology, and transcription services. A bachelor's degree is required with a master's degree, preferred. 3-5 years experience, managing all aspects of anatomic pathology is required. The ideal candidate will be collaborative and positive and bring innovative ideas. This is an opportunity to be involved in a complete renovation and redesign of the department. Special features of the position: Reporting to the Vice President of Laboratory Services, this newly created position will be a key member of the leadership team and manage the continued growth of the department, especially in the area of molecular testing. SLONEPARTNERS THERESE COOK - SENIOR EXECUTIVE RECRUITER Corporate Headquarters 1521 Alton Road #638 Miami Beach, Florida 33139 TOLL FREE: 877.419.1439 DIRECT: 330.863.1054 CELL: 330.323.4525 www.slonepartners.com On Jan 24, 2013, at 1:00 PM, histonet-request@lists.utsouthwestern.edu wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Fw: [Histonet] Re: Question (Rene J Buesa) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Thu, 24 Jan 2013 09:44:00 -0800 (PST) > From: Rene J Buesa > Subject: Fw: [Histonet] Re: Question > To: Histonet > Message-ID: > <1359049440.20318.YahooMailNeo@web163102.mail.bf1.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > We have to remember that it is not just putting slides in/out of automated stainer. > It is also understanding what the histotech is doing, how to design a validation experiment, how to look for an antibody the pathologists wants to test, how to troubleshoot a problem, how to justify switching from a detection system to another more convenient to the procedure on hand that for any reason no longer "works". > A histotech needs to know what s/he is doing. And to do all of the above has to have knowledge besides the training and the mechanics of what s/he is doing. > Designating a task as "high complexity" demands that the histotech has the basic theoretical knowledge and therefore deserves a better pay. > As some people has said before, putting slides in/out of an automated stainer of any kind) can be done by a monkey. > Understanding what is involved in IHC is above a monkey's pay grade. > Ren? J. > > From: "histotech@imagesbyhopper.com" > To: "'Sullivan, Beatrice'" ; 'Mark Tarango' ; 'Tim Higgins' > Cc: histonet@lists.utsouthwestern.edu; courtney.pierce@quintiles.com > Sent: Thursday, January 24, 2013 11:18 AM > Subject: RE: [Histonet] Re: Question > > It also depends on the State you are in. Florida considers this a high > complexity test and requires that a licensed technologist either do the test > or oversee a licensed technician who is performing the test. > >>> From my view, especially with all the automatic stainers out there, simply > putting the slides on/off the stainer should not be considered high > complexity! The interpretation of the stain should be done by a trained > individual though. > > Just my $0.02. > > Michelle > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sullivan, > Beatrice > Sent: Thursday, January 24, 2013 7:57 AM > To: Mark Tarango; Tim Higgins > Cc: histonet@lists.utsouthwestern.edu; courtney.pierce@quintiles.com > Subject: RE: [Histonet] Re: Question > > I called CAP a short time ago about who was qualified to run these. I was > told then that there was no requirement for a degreed person to run these. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Tarango > Sent: Wednesday, January 23, 2013 4:20 PM > To: Tim Higgins > Cc: histonet@lists.utsouthwestern.edu; courtney.pierce@quintiles.com > Subject: Re: [Histonet] Re: Question > > If anyone has any documentation that says the staining of IHC slides is NOT > high complexity it would help a histonetter out there. I got an e-mail from > someone who is HT(ASCP)QIHC but does not have an AA degree. Their lab > director is threatening their job saying they aren't qualified to do IHC > staining. If anyone has something to refer to it would be helpful for this > person. I already suggested contacting CAP and getting a written response. > > I believe IHC is high complexity but not the staining portion. Since no > result is being produced by the IHC tech how can this be high complexity? > > thanks > > Mark > > On Wed, Jan 23, 2013 at 11:44 AM, Tim Higgins wrote: > >> >> The professional interpretation is considered a high complexity test >> but not the actual technical component. >> >> >> >> Thanks, >> >> >> >> Timothy N. Higgins, HT (ASCP), QIHC >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > This message, and any included attachments, are from Virtua Health or its > related affiliates and is intended only for the addressee(s). The > information contained herein is privileged, proprietary or may include > confidential information and/or protected patient health information. Any > unauthorized review, forwarding, printing, copying, distributing, or > otherwise disseminating or taking any action based on such information is > strictly prohibited. If you have received this message in error, or have > reason to believe you are not authorized to receive it, please delete this > message promptly and notify the sender by e-mail with a copy to > ISSECURITY@virtua.org. > > Thank you > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ----- > No virus found in this message. > Checked by AVG - http://www.avg.com/ > Version: 2013.0.2890 / Virus Database: 2639/6052 - Release Date: 01/23/13 > > ----- > No virus found in this message. > Checked by AVG - www.avg.com > Version: 2013.0.2890 / Virus Database: 2639/6054 - Release Date: 01/24/13 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 110, Issue 31 > ***************************************** > SLONEPARTNERS THERESE COOK - SENIOR EXECUTIVE RECRUITER Corporate Headquarters 1521 Alton Road #638 Miami Beach, Florida 33139 TOLL FREE: 877.419.1439 DIRECT: 330.863.1054 CELL: 330.323.4525 www.slonepartners.com From Caroline.Pratt <@t> uphs.upenn.edu Thu Jan 24 14:15:33 2013 From: Caroline.Pratt <@t> uphs.upenn.edu (Pratt, Caroline) Date: Thu Jan 24 14:15:44 2013 Subject: [Histonet] Re: Question In-Reply-To: <1359049355.10322.YahooMailNeo@web163102.mail.bf1.yahoo.com> References: <6932520047F7EE46B512E9801344F160038CC5@ExchangeMB-1.Virtua.org><000301cdfa4e$79577420$6c065c60$@imagesbyhopper.com> <1359049355.10322.YahooMailNeo@web163102.mail.bf1.yahoo.com> Message-ID: For example, we have a 35+ year Immunotech with a Bachelors of Science who learned bench techniques by hand, when the automated stainer malfunctions, she is able to identify how to pick up where the machine left off and complete the test, saving the tissue. As Rene said, by understanding the science and theory behind the mechanics, you improve patient outcomes and are capable of high complexity testing even if you have mechanic assistance under most circumstances. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, January 24, 2013 12:43 PM To: histotech@imagesbyhopper.com; 'Sullivan, Beatrice'; 'Mark Tarango'; 'Tim Higgins' Cc: histonet@lists.utsouthwestern.edu; courtney.pierce@quintiles.com Subject: Re: [Histonet] Re: Question We have to remember that it is not just putting slides in/out of automated stainer. It is also understanding what the histotech is doing, how to design a validation experiment, how to look for an antibody the pathologists wants to test, how to troubleshoot a problem, how to justify switching from a detection system to another more convenient to the procedure on hand that for any reason no longer "works". A histotech needs to know what s/he is doing. And to do all of the above has to have knowledge besides the training and?the mechanics of what s/he is doing. Designating a task as "high complexity" demands that the histotech has the basic theoretical knowledge and therefore deserves a better pay. As some people has said before, putting slides in/out of an automated stainer of any kind) can be done by a monkey. Understanding what is involved in IHC is above a monkey's pay grade. Ren? J. From: "histotech@imagesbyhopper.com" To: "'Sullivan, Beatrice'" ; 'Mark Tarango' ; 'Tim Higgins' Cc: histonet@lists.utsouthwestern.edu; courtney.pierce@quintiles.com Sent: Thursday, January 24, 2013 11:18 AM Subject: RE: [Histonet] Re: Question It also depends on the State you are in.? Florida considers this a high complexity test and requires that a licensed technologist either do the test or oversee a licensed technician who is performing the test. >>From my view, especially with all the automatic stainers out there, simply putting the slides on/off the stainer should not be considered high complexity!? The interpretation of the stain should be done by a trained individual though. Just my $0.02. Michelle -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sullivan, Beatrice Sent: Thursday, January 24, 2013 7:57 AM To: Mark Tarango; Tim Higgins Cc: histonet@lists.utsouthwestern.edu; courtney.pierce@quintiles.com Subject: RE: [Histonet] Re: Question I called CAP a short time ago about who was qualified to run these. I was told then that there was no requirement for a degreed person to run these. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Tarango Sent: Wednesday, January 23, 2013 4:20 PM To: Tim Higgins Cc: histonet@lists.utsouthwestern.edu; courtney.pierce@quintiles.com Subject: Re: [Histonet] Re: Question If anyone has any documentation that says the staining of IHC slides is NOT high complexity it would help a histonetter out there.? I got an e-mail from someone who is HT(ASCP)QIHC but does not have an AA degree.? Their lab director is threatening their job saying they aren't qualified to do IHC staining.? If anyone has something to refer to it would be helpful for this person.? I already suggested contacting CAP and getting a written response. I believe IHC is high complexity but not the staining portion.? Since no result is being produced by the IHC tech how can this be high complexity? thanks Mark On Wed, Jan 23, 2013 at 11:44 AM, Tim Higgins wrote: > > The professional interpretation is considered a high complexity test > but not the actual technical component. > > > > Thanks, > > > > Timothy N. Higgins, HT (ASCP), QIHC > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message, and any included attachments, are from Virtua Health or its related affiliates and is intended only for the addressee(s). The information contained herein is privileged, proprietary or may include confidential information and/or protected patient health information. Any unauthorized review, forwarding, printing, copying, distributing, or otherwise disseminating or taking any action based on such information is strictly prohibited. If you have received this message in error, or have reason to believe you are not authorized to receive it, please delete this message promptly and notify the sender by e-mail with a copy to ISSECURITY@virtua.org. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----- No virus found in this message. Checked by AVG - http://www.avg.com/ Version: 2013.0.2890 / Virus Database: 2639/6052 - Release Date: 01/23/13 ----- No virus found in this message. Checked by AVG - www.avg.com Version: 2013.0.2890 / Virus Database: 2639/6054 - Release Date: 01/24/13 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From king.laurie <@t> marshfieldclinic.org Thu Jan 24 15:15:53 2013 From: king.laurie <@t> marshfieldclinic.org (King, Laurie J) Date: Thu Jan 24 15:15:52 2013 Subject: [Histonet] job opening Message-ID: <7578207839F50248A7A6CD33517295EA12BF8BB1@MCL-EXMB03.mfldclin.org> Posting this for a colleague. Full time opening for Histology at The Diagnostic and Treatment Center in Weston Wisconsin. For details go to their website at www.dxandtx.com Laurie King ______________________________________________________________________ The contents of this message may contain private, protected and/or privileged information. If you received this message in error, you should destroy the e-mail message and any attachments or copies, and you are prohibited from retaining, distributing, disclosing or using any information contained within. Please contact the sender and advise of the erroneous delivery by return e-mail or telephone. Thank you for your cooperation. From Margaret.Perry <@t> sdstate.edu Thu Jan 24 15:29:25 2013 From: Margaret.Perry <@t> sdstate.edu (Perry, Margaret) Date: Thu Jan 24 15:29:52 2013 Subject: [Histonet] Nile Red dye Message-ID: <25F4FBA34BE9D142964ECC4525B82AEE047483@SDSU-EX03.jacks.local> Is there another name for Nile Red? Could it be a florescent dye? I couldn't find it on the Biological Stain commission or in a reference book from Harleco. I don't know what the stain will be used for. Margaret Perry HT(ASCP) Veterinary & Biomedical Sciences Department North Campus Drive Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 From llewllew <@t> shaw.ca Thu Jan 24 17:46:37 2013 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Thu Jan 24 17:46:49 2013 Subject: [Histonet] Nile Red dye In-Reply-To: <25F4FBA34BE9D142964ECC4525B82AEE047483@SDSU-EX03.jacks.local> References: <25F4FBA34BE9D142964ECC4525B82AEE047483@SDSU-EX03.jacks.local> Message-ID: <5101C7DD.9080500@shaw.ca> Nile red is formed from Nile blue and is used to stain lipids. http://stainsfile.info/StainsFile/dyes/51180.htm Bryan Llewellyn Perry, Margaret wrote: > Is there another name for Nile Red? Could it be a florescent dye? I couldn't find it on the Biological Stain commission or in a reference book from Harleco. I don't know what the stain will be used for. > > Margaret Perry HT(ASCP) > Veterinary & Biomedical Sciences Department > North Campus Drive Box 2175 > South Dakota State University > Brookings SD 57007 > 605-688-5638 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From relia1 <@t> earthlink.net Fri Jan 25 10:30:52 2013 From: relia1 <@t> earthlink.net (Pam Barker) Date: Sat Jan 26 11:07:11 2013 Subject: [Histonet] RELIA Hot Histology Job Alert!! 01/25/2013 Lead IHC Tech NY, NY Message-ID: <00ac01cdfb19$57f59980$07e0cc80$@earthlink.net> Hi Histonetters!! TGIF!!! I have a new position that I want to tell you about: RELIA Solutions has been engaged to assist a presitigious medical facility located in NYC in their recruitment of a Lead Immunohistochemistry Tech. This position requires ASCP certification HT/HTL (QIHC a plus) and NYS licensure. A minimum of a Bachelor's Degree and 2-5 years experience performing immunohistochemistry. This is a permanent full time day shift position and my client offers excellent compensation, outstanding benefits and and exemplary environment that can't be beat! If this is the right job for you RELIA can make it happen! For more information please contact Pam Barker at relia1@earthlink.net or toll free at 866-607-3542 RELIA Solutions is the nation's ONLY recruiting firm specializing in the nationwide permanent placement of histology professionals. To sign up for our free histology careers bulletin please send an e-mail to relia1@earthlink.net and include subscribe in the subject line. Keywords: Histology, histologist, histotechnician, histotechnologist, immunohistochemistry, IHC Happy New Year!! Thank You! Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From relia1 <@t> earthlink.net Fri Jan 25 11:14:14 2013 From: relia1 <@t> earthlink.net (Pam Barker) Date: Sat Jan 26 11:22:42 2013 Subject: [Histonet] RELIA Hot Histology Job Alert!! 01/25/2013 Lead IHC Tech NY, NY Message-ID: <00c701cdfb1f$66f51c70$34df5550$@earthlink.net> Hi Histonetters!! TGIF!!! I have a new position that I want to tell you about: RELIA Solutions has been engaged to assist a presitigious medical facility located in NYC in their recruitment of a Lead Immunohistochemistry Tech. This position requires ASCP certification HT/HTL (QIHC a plus) and NYS licensure. A minimum of a Bachelor's Degree and 2-5 years experience performing immunohistochemistry. This is a permanent full time day shift position and my client offers excellent compensation, outstanding benefits and and exemplary environment that can't be beat! If this is the right job for you RELIA can make it happen! For more information please contact Pam Barker at relia1@earthlink.net or toll free at 866-607-3542 RELIA Solutions is the nation's ONLY recruiting firm specializing in the nationwide permanent placement of histology professionals. To sign up for our free histology careers bulletin please send an e-mail to relia1@earthlink.net and include subscribe in the subject line. Keywords: Histology, histologist, histotechnician, histotechnologist, immunohistochemistry, IHC Happy New Year!! Thank You! Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From Pat.Patterson <@t> propath.com Fri Jan 25 12:46:42 2013 From: Pat.Patterson <@t> propath.com (Pat Patterson) Date: Sat Jan 26 11:44:45 2013 Subject: [Histonet] IHC Job in Dallas Message-ID: <82C7248978CB50469FD6BA68EBBEFE67095F3A60@exchange.propathlab.com> IMMUNOHISTOCHEMISTRY TECHNICIAN ProPath, a progressive, CAP accredited, high-volume pathology practice in Dallas, Texas is seeking an Immunohistochemistry Technician for its' Immunohistochemistry Lab. Responsibilities include slide preparation (paraffin and frozen sections), IHC staining using our unique manual system, antibody titer preparation, equipment maintenance, supply/reagent inventory maintenance, and QC/QA recording. The ideal candidate will have a minimum of 4 years Histology experience with paraffin microtomy with a variety of different tissue types, prefer at least 1 year immunohistochemistry, immunofluorescence or in situ hybridization and frozen section experience. Working knowledge of IHC theory required, hands on IHC performance desired. If using an automated system we'll easily train you on our manual system. HT (ASCP) or QIHC desired. The hours for the position are 5:30 p.m. to 2:00 a.m. Monday through Friday. ProPath utilizes leading technology and is a quality oriented pathology laboratory. Benefits include medical, dental, Short and Long Term Disability insurance, a matched 401K plan and more! Don't Follow the Leader! Join the Leader! To apply, please visit www.propath.com EOE Accessibility Accommodations If you require an accommodation to navigate or apply to our careers site, please send your request to accessibility@propath.com. Pat Patterson, HTL(ASCP) Supervisor, Immunohistochemistry ProPath - The Leader in Pathology Services 1355 River Bend Drive Dallas, TX 75247 214-237-1700 x 2027 214-237-1730 fax To learn more about ProPath, please visit http://www.ProPath.com This electronic message is intended to be for the use only of the named recipient and may contain information that is confidential or privileged. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this message in error or are not the named recipient, please notify us immediately by contacting the sender at the electronic mail address noted above, and delete and destroy all copies of this message. From celebrej <@t> HHSC.CA Fri Jan 25 13:00:57 2013 From: celebrej <@t> HHSC.CA (Celebre Julia) Date: Sat Jan 26 11:47:04 2013 Subject: [Histonet] SDH methods for muscles Message-ID: <722701352B76E841BFB9EFAF72553CD2A09E9E@ipexmailm02.hhsc.ca> I give up!! One week it works, the next week it doesn't, same reagents, same method, same tech, for the life of me I can't figure out what is the problem. If anybody has a fail safe method they are willing to share, please send it along. I'm pretty sure my neuropathologist will appreciate all the help I can get. Julia Celebre Sr MLT Anatomic Pathology Hamilton General Hospital 905-527-4322 ext 46179 This information is directed in confidence solely to the person named above and may not otherwise be distributed, copied or disclosed. Therefore, this information should be considered strictly confidential. If you have received this email in error, please notify the sender immediately via a return email for further direction. Thank you for your assistance. From thigginsht <@t> msn.com Fri Jan 25 13:04:05 2013 From: thigginsht <@t> msn.com (Tim Higgins) Date: Sat Jan 26 11:49:23 2013 Subject: [Histonet] Re: Question In-Reply-To: References: Message-ID: All I have to go by is CAP's response to the question, "Is IHC a High Complexity Test". Their verbal answer is no, but there is nothing formally documented. The only documented statement is from 2010 Webinar during the Question and Answer portion. It is as follows, Laboratory Accreditation Program CAP Personnel Qualifications and Personnel Requirements Webinar March 17, 2010 Q & A (Skipping to question 20) Question: 20. What are the CLIA/CAP requirements for histology staff? Is histology considered high complexity? Is just an HT acceptable or do they need to have an associates to perform routine histology, IHC, IF, ISH, etc.? Answer: Histology processing such as staining or cutting is not covered under CLIA. However, any type of grossing is considered high complexity testing. This even includes very small specimens. There will be a modified checklist requirement that further clarifies this in the June 2010 Checklist edition. Sorry thats all I got. Thanks, Timothy N. Higgins, HT (ASCP), QIHC From Rebecca.Riesen <@t> hma.com Fri Jan 25 13:21:49 2013 From: Rebecca.Riesen <@t> hma.com (Riesen, Rebecca) Date: Sat Jan 26 11:50:07 2013 Subject: [Histonet] RE: Histonet Digest, Vol 110, Issue 22 In-Reply-To: <19740412024625.776E2411482121E7@mx2.hma.com> References: <19740412024625.776E2411482121E7@mx2.hma.com> Message-ID: My Doc's don't like the "just ignore it" answer. They want me to "just fix it"! I repeat/ I tweak/ and we move on until the next one pops up and then we start all over again with the same discussion. I would really appreciate some kind of "official" answer from some vendors development teams. We (histologists) really deserve an answer. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Wednesday, January 16, 2013 1:00 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 110, Issue 22 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Helicobacter pylori immunocytochemistry - cytoplasmatic staining (Bob Richmond) ---------------------------------------------------------------------- Message: 1 Date: Wed, 16 Jan 2013 11:18:30 -0500 From: Bob Richmond Subject: [Histonet] Re: Helicobacter pylori immunocytochemistry - cytoplasmatic staining To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 I quite often see faint cytoplasmic staining in immunostains for Helicobacter. You're supposed to ignore it. It's often present in the negative control slide - one of the few reasons I ever look at a negative control slide. These days I get my IHC for Helicobacter from whatever Genzyme is called this week. I'm noticing considerably less cytoplasmic staining than I've usually seen before. (I have no commercial interest here.) Bob Richmond Samurai Pathologist Maryville TN ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 110, Issue 22 ***************************************** From mbmphoto <@t> gmail.com Fri Jan 25 18:02:39 2013 From: mbmphoto <@t> gmail.com (Maria Mejia) Date: Sat Jan 26 12:51:51 2013 Subject: [Histonet] surgipath water bath model 04535S Message-ID: <42AAEF27-A56F-44A9-AFF7-0483667ABA11@gmail.com> Hello, Please can anyone provide working reviews (pros & cons) of the following used item; Surgipath water bath model 04535S. I found this unit on the American Instrument Exchange (AIE) for a very good deal. I would greatly appreciate any information you can provide. Maria Mejia Histology Manager/Supervisor Affrymetrix, Inc From madeleinehuey <@t> gmail.com Sat Jan 26 23:58:16 2013 From: madeleinehuey <@t> gmail.com (Madeleine Huey) Date: Sat Jan 26 23:58:24 2013 Subject: [Histonet] Re: Histonet Digest, Vol 110, Issue 33 In-Reply-To: <510419d5.290fb60a.2d9d.ffffbdbaSMTPIN_ADDED_MISSING@mx.google.com> References: <510419d5.290fb60a.2d9d.ffffbdbaSMTPIN_ADDED_MISSING@mx.google.com> Message-ID: Message: 2 Date: Fri, 25 Jan 2013 19:00:57 +0000 From: Celebre Julia Subject: [Histonet] SDH methods for muscles To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <722701352B76E841BFB9EFAF72553CD2A09E9E@ipexmailm02.hhsc.ca> Content-Type: text/plain; charset="us-ascii" I give up!! One week it works, the next week it doesn't, same reagents, same method, same tech, for the life of me I can't figure out what is the problem. If anybody has a fail safe method they are willing to share, please send it along. I'm pretty sure my neuropathologist will appreciate all the help I can get. Julia Celebre Sr MLT Anatomic Pathology Hamilton General Hospital 905-527-4322 ext 46179 Julia, What is the actual problem that you are having? Are you having IHC problem? Paraffin or frozen tissues? How do you perform them in your lab? Manual or automated (which platform)? Off line or on line antigen retrieval (which method)? Maybe we can help you if you give us a more detail with your procedure. madeleine_h@elcaminohospital.org From ibernard <@t> uab.edu Sun Jan 27 13:29:04 2013 From: ibernard <@t> uab.edu (Ian R Bernard) Date: Sun Jan 27 13:29:10 2013 Subject: [Histonet] CSF GYN Testing Message-ID: What is a perfect amt/size specimen for CSF for Cytology non gyn testing per the literature? Ian R. Bernard Ian R. Bernard, MSHA, HT (ASCP) NCOIC-Manager, Anatomic Pathology Lab 10th Medical Group USAF Academy, CO 80840 Graduate Certificate In Gerontology Student-UAB 210-687-7540 From lbest <@t> sunriselab.com Mon Jan 28 06:36:51 2013 From: lbest <@t> sunriselab.com (Laurie Best) Date: Mon Jan 28 10:21:42 2013 Subject: [Histonet] Thrombin Message-ID: <4A672C6AE0402D4A89ECE29E8A4B47E30529BF07@MailPDC.sunriselab.com> I am trying to purchase thrombin for cell block preparation, and have been running into dead ends. Can someone give me their vendor information? Laurie Best Histology Supervisor Sunrise Medical Labs 250 Miller Place Hicksville, NY 11801 (631)435-1515x1018 "This message contains privileged and confidential information intended only for the use of the addressee named above. If you are not the intended recipient of this message you must not disseminate, copy or take any action in reliance on it." From candice_camille <@t> yahoo.com Mon Jan 28 08:26:19 2013 From: candice_camille <@t> yahoo.com (Candice Smoots) Date: Mon Jan 28 13:58:11 2013 Subject: [Histonet] SDH methods for muscles In-Reply-To: <722701352B76E841BFB9EFAF72553CD2A09E9E@ipexmailm02.hhsc.ca> References: <722701352B76E841BFB9EFAF72553CD2A09E9E@ipexmailm02.hhsc.ca> Message-ID: <1359383179.59685.YahooMailNeo@web165003.mail.bf1.yahoo.com> Hey Julia... ? WE do SDH routniely on our muscles every week and they work just fine. Can you forward your protocol? Id like to see the difference in them if any? I remain yours truely, Candice Camille ________________________________ From: Celebre Julia To: "'histonet@lists.utsouthwestern.edu'" Sent: Friday, January 25, 2013 1:00 PM Subject: [Histonet] SDH methods for muscles I give up!! One week it works, the next week it doesn't, same reagents, same method, same tech, for the life of me I can't figure out what is the problem. If anybody has a fail safe method they are willing to share, please send it along. I'm pretty sure my neuropathologist will appreciate all the help I can get. Julia Celebre? Sr MLT Anatomic Pathology Hamilton General Hospital 905-527-4322? ext 46179 This information is directed in confidence solely to the person named above and may not otherwise be distributed, copied or disclosed. Therefore, this information should be considered strictly confidential. If you have received this email in error, please notify the sender immediately via a return email for further direction. Thank you for your assistance. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nicole <@t> dlcjax.com Mon Jan 28 10:32:51 2013 From: nicole <@t> dlcjax.com (Nicole Tatum) Date: Mon Jan 28 17:16:05 2013 Subject: [Histonet] Shandon HistoCentre II question Message-ID: <3363.208.62.167.196.1359390771.squirrel@webmail.realpages.com> I know most embedding machines put out heat due to the block warming chamber, paraffin vat and compressor of cooling consol. But those of you who have experience with this machine, do you think it seems hotter than other or does it have issues with retaining a constant temperature? Any thoughts.. Nicole Tatum, HTL ASCP From lcolbert <@t> pathmdlabs.com Mon Jan 28 10:42:11 2013 From: lcolbert <@t> pathmdlabs.com (Laurie Colbert) Date: Mon Jan 28 17:20:30 2013 Subject: [Histonet] Robin Simpkins Message-ID: <12ECD7346266D74691EC2BFC75285E4501199C62@BFL323E10.pathmdlabs.local> Does anyone have the phone number and/or email for Robin Simpkins at Biocare? Thanks, Laurie Colbert From hans <@t> histologistics.com Mon Jan 28 10:51:26 2013 From: hans <@t> histologistics.com (Hans B Snyder) Date: Mon Jan 28 17:20:34 2013 Subject: [Histonet] TBS tissue processor single mode vs group mode Message-ID: Hello All, We just got a TBS APT1-120 tissue processor donated to us. I am would like to hear from anyone about the single mode verse the group mode of operation. What your opinoins about using one verse the other. I hope to set this up this week and would like advice on which is better. Thank you -- Hans B Snyder Histologistics 100 Barber Ave Worcester, MA 01606 508-308-7800 hans@histologistics.com From Nagy_Natalie <@t> holyokehealth.com Mon Jan 28 11:40:12 2013 From: Nagy_Natalie <@t> holyokehealth.com (Natalie Nagy) Date: Mon Jan 28 17:30:42 2013 Subject: [Histonet] Question about slide drying/ convection ovens Message-ID: <510671AC0200000F00333F03@hh11.holyokehealth.com> Hi everyone, Just have a quick question for all of you out there in histo-land. Have you ever bought or worked with a gravity convection oven (the thermo shandon 20GC), for drying of slides?? If so..how was it? Did it properly dry all of your slides, did it keep temp?? I am thinking about buying one, and wanted some opinions. Or do you all prefer a real forced air slide drying oven? Thanks in advance, Natalie Nagy (HT)ASCP Histology Supervisor Holyoke Medical Center Holyoke, MA. CONFIDENTIALITY NOTICE: This email communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please reply to the sender immediately and destroy all copies of this communication and any attachments. For further information regarding Holyoke Medical Center's privacy policy, Please visit our Internet web site at http://www.holyokehealth.com From Reuel.Cornelia <@t> tsrh.org Mon Jan 28 11:47:00 2013 From: Reuel.Cornelia <@t> tsrh.org (Reuel Cornelia) Date: Mon Jan 28 17:33:44 2013 Subject: [Histonet] Thermo Scientific STP 420ES Tissue Processor Message-ID: <5106654A.077E.00C5.1@tsrh.org> Hello histonetter, Do you have any feedback on the efficiency of the Thermo Scientific STP 420ES Tissue Processor. We are planning to purchase a tissue processor that will meet our goal in reducing the time of bone tissue processing that we use both for plastic(MMA) and paraffin research and clinical tissue. We will be having our demo instrument on tomorrow. Any feedback, or opinion regarding this processor will be of great help. Thank you. Reuel Cornelia, BS MT, AMT Cellular Pathology Texas Scottish Rite Hospital for Children 2222 Welborn Street Dallas, TX 75219 Tel: 214-559-7766 fax: 214-559-7768 From rjbuesa <@t> yahoo.com Mon Jan 28 12:05:50 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Jan 28 17:37:20 2013 Subject: [Histonet] Question about slide drying/ convection ovens In-Reply-To: <510671AC0200000F00333F03@hh11.holyokehealth.com> References: <510671AC0200000F00333F03@hh11.holyokehealth.com> Message-ID: <1359396350.49812.YahooMailNeo@web163105.mail.bf1.yahoo.com> Natalia: I just cannot understand the concept of "gravity" convection oven because if you say "gravity" by definition that will mean that the heat will work by gravity and any heated air goes, also by definition, against gravity because any heat air will go UP and not down, hence the "hot air balloons". Probably the title is just a "catchy" but wrongly selected sales mimic, something "new and different". Other than that, and going to your question, no I have never used such an oven. Ovens are of the convection type and in all of them the heating elements are at the bottom and the heated air goes UP. Ren? J. From: Natalie Nagy To: histonet@lists.utsouthwestern.edu Sent: Monday, January 28, 2013 12:40 PM Subject: [Histonet] Question about slide drying/ convection ovens Hi everyone, ? ? ? ? ? ? ? ? ? Just have a quick question for all of you out there in histo-land. Have you ever bought or worked with a gravity convection oven (the thermo shandon 20GC), for drying of slides?? If so..how was it? Did it properly dry all of your slides, did it keep temp?? I am thinking about buying one, and wanted some opinions. Or do you all prefer a real forced air slide drying oven? Thanks in advance, ? ? ? ? ? ? ? ? ? ? Natalie Nagy (HT)ASCP Histology Supervisor Holyoke Medical Center Holyoke, MA. CONFIDENTIALITY NOTICE: This email communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please reply to the sender immediately and destroy all copies of this communication and any attachments. For further information regarding Holyoke Medical Center's privacy policy, Please visit our Internet web site at http://www.holyokehealth.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Nagy_Natalie <@t> holyokehealth.com Mon Jan 28 12:30:55 2013 From: Nagy_Natalie <@t> holyokehealth.com (Natalie Nagy) Date: Mon Jan 28 17:41:10 2013 Subject: [Histonet] Thrombin&In-Reply-To= Message-ID: <51067D8F0200000F00333F0D@hh11.holyokehealth.com> Dear Laurie, We use thrombin and plasma to form our cell blocks. We order the Topical Thrombin 5,000 IU from King Pharmaceuticals. Hope this helps, Natalie Nagy (HT)ASCP Holyoke Hospital Holyoke, MA. CONFIDENTIALITY NOTICE: This email communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please reply to the sender immediately and destroy all copies of this communication and any attachments. For further information regarding Holyoke Medical Center's privacy policy, Please visit our Internet web site at http://www.holyokehealth.com From Timothy.Morken <@t> ucsfmedctr.org Mon Jan 28 12:41:14 2013 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Mon Jan 28 17:41:11 2013 Subject: [Histonet] Question about slide drying/ convection ovens In-Reply-To: <1359396350.49812.YahooMailNeo@web163105.mail.bf1.yahoo.com> References: <510671AC0200000F00333F03@hh11.holyokehealth.com> <1359396350.49812.YahooMailNeo@web163105.mail.bf1.yahoo.com> Message-ID: <761E2B5697F795489C8710BCC72141FF0530FD@ex07.net.ucsf.edu> There is a reason to distinguish the type of oven but the terms are used loosely and so cause confusion. "Convection" is used to describe in nature to describe the natural circulation of heat and can be of "natural" gravity convection in which heat rises, or forced in which some kind of external force spreads the heat "faster" Ie, wind. Strictly speaking a "Convection Oven" is one that uses a fan to distribute the heat. That is opposed to Conventional Ovens that do not use fans and rely on gravity to distribute heat (heat rises, setting up convection currents). However, if you look at equipment catalogs you will see terms like "Gravity Convection" and "Forced Air" to distinguish between ovens without and with fans respectively. Most ovens in use are "gravity-convection" ovens that rely on the natural rise of heated air to circulate the heat. They don' t use fans. In contrast is the forced-air convection oven that uses a fan to circulate air and ensure even heating throughout the oven. Forced air can heat/dry a sample faster if the fan is set to circulate air at a faster rate than would happen by natural convection. So, the moral of the story is to read the description of the oven to find out exactly what kind of oven it really is! Tim Morken UCSF Pathology -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Monday, January 28, 2013 10:06 AM To: Natalie Nagy; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Question about slide drying/ convection ovens Natalia: I just cannot understand the concept of "gravity" convection oven because if you say "gravity" by definition that will mean that the heat will work by gravity and any heated air goes, also by definition, against gravity because any heat air will go UP and not down, hence the "hot air balloons". Probably the title is just a "catchy" but wrongly selected sales mimic, something "new and different". Other than that, and going to your question, no I have never used such an oven. Ovens are of the convection type and in all of them the heating elements are at the bottom and the heated air goes UP. Ren? J. From: Natalie Nagy To: histonet@lists.utsouthwestern.edu Sent: Monday, January 28, 2013 12:40 PM Subject: [Histonet] Question about slide drying/ convection ovens Hi everyone, ? ? ? ? ? ? ? ? ? Just have a quick question for all of you out there in histo-land. Have you ever bought or worked with a gravity convection oven (the thermo shandon 20GC), for drying of slides?? If so..how was it? Did it properly dry all of your slides, did it keep temp?? I am thinking about buying one, and wanted some opinions. Or do you all prefer a real forced air slide drying oven? Thanks in advance, ? ? ? ? ? ? ? ? ? ? Natalie Nagy (HT)ASCP Histology Supervisor Holyoke Medical Center Holyoke, MA. CONFIDENTIALITY NOTICE: This email communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please reply to the sender immediately and destroy all copies of this communication and any attachments. For further information regarding Holyoke Medical Center's privacy policy, Please visit our Internet web site at http://www.holyokehealth.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From christina.thurby <@t> bms.com Mon Jan 28 13:00:38 2013 From: christina.thurby <@t> bms.com (Thurby, Christina) Date: Mon Jan 28 17:44:12 2013 Subject: [Histonet] RE: CSF non-GYN testing In-Reply-To: References: Message-ID: Hi Ian, You have a good question but can you tell us a little more about your specimen type? Are you processing these specimens in a fluid based medium (ie, Thin Prep) or making some cytospins? Any types of bodily fluids that are collected will vary in cellularity and any microbes that could be potentially present in the patient sample. It is very difficult to pinpoint a volume that would be appropriate for collection. Sorry if you already know this - CSF samples are generally collected in multiple tubes and divided through the various departments of the clinical lab and histology based on the battery of tests the physician has requested. Particularly for CSF, I would request that any and all available remaining samples from the clinical laboratory be given to histology for processing in addition to the histology aliquot for processing. Some of the CSF samples are very acellular and you'll have a better chance at identifying whatever disease process may be present with a greater volume of CSF sample. Christina Thurby Bristol Myers Squibb 812-307-2093 >Message: 1 >Date: Sun, 27 Jan 2013 19:29:04 +0000 >From: Ian R Bernard >Subject: [Histonet] CSF GYN Testing >To: "histonet@lists.utsouthwestern.edu" > >Message-ID: > >Content-Type: text/plain; charset="us-ascii" > >What is a perfect amt/size specimen for CSF for Cytology non gyn testing >per the literature? > >Ian R. Bernard >Ian R. Bernard, MSHA, HT (ASCP) >NCOIC-Manager, Anatomic Pathology Lab >10th Medical Group >USAF Academy, CO 80840 >Graduate Certificate In Gerontology Student-UAB >210-687-7540 > > > This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. From dmborel <@t> aol.com Mon Jan 28 13:10:12 2013 From: dmborel <@t> aol.com (Dmborel) Date: Mon Jan 28 17:44:14 2013 Subject: [Histonet] Oil red O stain on formalin fixed liver Message-ID: <8CFCBA23B5168C7-1954-33FA2@webmail-d018.sysops.aol.com> We have an autopsy on a young child. I have a working diagnosis of possible Reye's syndrome and need an Oil Red O stain on a section of formalin fixed liver (not processed) to confirm microvesicular hepatic steatosis. Does anyone do this stain, and could I send the tissue or unstained slides for you to perform the stain and send islides back for me to interpret? I can provide a bone marrow smear to use as a positive control slide, pay shipping both directions and a technical fee. Thank you, David M. Borel, M.D. Pathology Services, P.A. Topeka, KS From rsrichmond <@t> gmail.com Mon Jan 28 16:02:23 2013 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Mon Jan 28 18:10:23 2013 Subject: [Histonet] Re: Thrombin for cell block preparations Message-ID: Laurie Best, Histology Supervisor at Sunrise Medical Labs, Hicksville NY asks: >>I am trying to purchase thrombin for cell block preparation, and have been running into dead ends. Can someone give me their vendor information?<< I know about using thrombin to clot plasma to form cytopathologists' cell blocks, but have never seen it done, and don't know a source. In the many labs I've worked in, I've found that cell block methods and results vary greatly from lab to lab, from labs that don't do them at all, to labs that do them routinely on pleural and peritoneal fluids with uniformly good results. If I were setting up a procedure, I'd probably try the commercial agar preparation Histogel. Cell blocks are a necessary cytologic preparation, the more so as the use of immunohistochemistry increases. Bob Richmond Samurai Pathologist Maryville TN From tony.henwood <@t> health.nsw.gov.au Mon Jan 28 16:03:32 2013 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Mon Jan 28 18:11:51 2013 Subject: [Histonet] RE: SDH methods for muscles In-Reply-To: <722701352B76E841BFB9EFAF72553CD2A09E9E@ipexmailm02.hhsc.ca> References: <722701352B76E841BFB9EFAF72553CD2A09E9E@ipexmailm02.hhsc.ca> Message-ID: <6D6BD1DE8A5571489398B392A38A71579D22CF93@xmdb04.nch.kids> I have found that the major problem with most of these enzymehistochemical stains is that the sections should not be allowed to sit too long at room temperature before staining. Either stain immediately on sectioning or store at -70oC. The enzymes tend to deteriorate quite rapidly at room temp. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Celebre Julia Sent: Saturday, 26 January 2013 6:01 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] SDH methods for muscles I give up!! One week it works, the next week it doesn't, same reagents, same method, same tech, for the life of me I can't figure out what is the problem. If anybody has a fail safe method they are willing to share, please send it along. I'm pretty sure my neuropathologist will appreciate all the help I can get. Julia Celebre Sr MLT Anatomic Pathology Hamilton General Hospital 905-527-4322 ext 46179 This information is directed in confidence solely to the person named above and may not otherwise be distributed, copied or disclosed. Therefore, this information should be considered strictly confidential. If you have received this email in error, please notify the sender immediately via a return email for further direction. Thank you for your assistance. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From tony.henwood <@t> health.nsw.gov.au Mon Jan 28 16:21:08 2013 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Mon Jan 28 18:15:00 2013 Subject: [Histonet] Oil red O stain on formalin fixed liver In-Reply-To: <8CFCBA23B5168C7-1954-33FA2@webmail-d018.sysops.aol.com> References: <8CFCBA23B5168C7-1954-33FA2@webmail-d018.sysops.aol.com> Message-ID: <6D6BD1DE8A5571489398B392A38A71579D22D03E@xmdb04.nch.kids> David, (From Doug Reye's old Laboratory). I have found the following technique the best: Cryotomy of Formalin Fixed Tissues Sometimes frozen sections are requested on tissue that has been fixed in 10% formalin. Cutting frozen sections of formalin fixed tissue can be frustrating at times due to shredding of the tissue or failure of the sections to stay on the slides. The following procedure makes sectioning a lot easier: Solutions: 1. OCT frozen embedding compound (or equivalent) 2. OCT Infiltration Solution: OCT Compound 4ml Distilled Water 8ml 3. Sticky Slides (Poly Lysine or equivalent) Method: 1. Take well-fixed tissue and gently wash in tap water 1hr. 2. Place in OCT Infiltration Solution and place on rotor. * For small pieces (needle or thin wedge) 2hr. * For larger pieces 4hrs - overnight. 3. Gently blot excess solution from tissue and embed in OCT and freeze. 4. Cut frozen sections and pick up on adhesive coated slides. 5. Place one slide in Methanol and stain H&E or air-dry sections for oil red O staining Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dmborel Sent: Tuesday, 29 January 2013 6:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Oil red O stain on formalin fixed liver We have an autopsy on a young child. I have a working diagnosis of possible Reye's syndrome and need an Oil Red O stain on a section of formalin fixed liver (not processed) to confirm microvesicular hepatic steatosis. Does anyone do this stain, and could I send the tissue or unstained slides for you to perform the stain and send islides back for me to interpret? I can provide a bone marrow smear to use as a positive control slide, pay shipping both directions and a technical fee. Thank you, David M. Borel, M.D. Pathology Services, P.A. Topeka, KS _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From Valerie.Hannen <@t> parrishmed.com Tue Jan 29 04:48:08 2013 From: Valerie.Hannen <@t> parrishmed.com (Hannen, Valerie) Date: Tue Jan 29 04:50:13 2013 Subject: FW: [Histonet] Re: Thrombin for cell block preparations Message-ID: <450B7A81EDA0C54E97C53D60F00776C3231B3D7BAF@isexstore03> -----Original Message----- From: Hannen, Valerie Sent: Tuesday, January 29, 2013 5:46 AM To: 'Bob Richmond' Subject: RE: [Histonet] Re: Thrombin for cell block preparations We get some Thromboplastin from the Hemo. Deparment and get Plasma from Blood Bank, when we need to make a cell block we add an equal amount of drops from both and let the specimen clot up. Hope this helps!! Valerie Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.hannen@parrishmed.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Monday, January 28, 2013 5:02 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Thrombin for cell block preparations Laurie Best, Histology Supervisor at Sunrise Medical Labs, Hicksville NY asks: >>I am trying to purchase thrombin for cell block preparation, and have >>been running into dead ends. Can someone give me their vendor >>information?<< I know about using thrombin to clot plasma to form cytopathologists' cell blocks, but have never seen it done, and don't know a source. In the many labs I've worked in, I've found that cell block methods and results vary greatly from lab to lab, from labs that don't do them at all, to labs that do them routinely on pleural and peritoneal fluids with uniformly good results. If I were setting up a procedure, I'd probably try the commercial agar preparation Histogel. Cell blocks are a necessary cytologic preparation, the more so as the use of immunohistochemistry increases. Bob Richmond Samurai Pathologist Maryville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet =================== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" =================== From sbaldwin <@t> mhhcc.org Tue Jan 29 06:59:15 2013 From: sbaldwin <@t> mhhcc.org (Sara Baldwin/mhhcc.org) Date: Tue Jan 29 07:00:46 2013 Subject: [Histonet] 88305 Message-ID: QUESTION HITONETTERS Does anyone no that the cuts of 88305 have been implemented?? All the research I have seen doesn't seem to state that it has been implemented Jan 1, 2013?? Thanks Histology/Cytology Supervisor S. Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph 812-996-0210, 0216, Fax 812-996-0232, Pager 812-481-0897, Cell 812-887-3357 From Joyce.Weems <@t> emoryhealthcare.org Tue Jan 29 08:41:13 2013 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Tue Jan 29 08:41:26 2013 Subject: [Histonet] 88305 In-Reply-To: References: Message-ID: I had this discussion with our Medicare reimbursement guru on the 11th. She says the reimbursement on CMS addendum B has not been reduced. (It is $38.10 - last year it was $36.84.) We are waiting to check reimbursement on a Medicare patient to see what the actual amount is. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sara Baldwin/mhhcc.org Sent: Tuesday, January 29, 2013 7:59 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] 88305 QUESTION HITONETTERS Does anyone no that the cuts of 88305 have been implemented?? All the research I have seen doesn't seem to state that it has been implemented Jan 1, 2013?? Thanks Histology/Cytology Supervisor S. Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph 812-996-0210, 0216, Fax 812-996-0232, Pager 812-481-0897, Cell 812-887-3357 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From hans <@t> histologistics.com Tue Jan 29 08:42:12 2013 From: hans <@t> histologistics.com (Hans B Snyder) Date: Tue Jan 29 08:42:17 2013 Subject: [Histonet] How do you label myoblasts and fibroblasts in cell culture to show up after paraffin processing? Message-ID: We are looking to differentiate between myoblasts and fibroblasts, Is there IHC or IF that can do this after paraffin processing? We were also wondering if it is possible to label these two prior to processing? Thank you all -- Hans B Snyder Histologistics 100 Barber Ave Worcester, MA 01606 508-308-7800 hans@histologistics.com From twebster <@t> CRH.org Tue Jan 29 09:50:35 2013 From: twebster <@t> CRH.org (Webster, Thomas S.) Date: Tue Jan 29 09:54:22 2013 Subject: [Histonet] 88305 Message-ID: <7207186ED68FB542803CAF1CE6E82FF8049480@exmb1.crh.org> They have been implemented is my understanding CONFIDENTIALITY NOTICE: This e-mail message, including all attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. You may NOT use, disclose, copy or disseminate this information. If you are not the intended recipient, please contact the sender by reply e-mail immediately. Please destroy all copies of the original message and all attachments. Your cooperation is greatly appreciated. Columbus Regional Hospital 2400 East 17th Street Columbus, Indiana 47201 From jerry.santiago <@t> bellsouth.net Tue Jan 29 10:48:59 2013 From: jerry.santiago <@t> bellsouth.net (=?utf-8?B?amVycnkuc2FudGlhZ29AYmVsbHNvdXRoLm5ldA==?=) Date: Tue Jan 29 10:49:10 2013 Subject: [Histonet] Florida Society for Histotechnology Message-ID: <310702.47624.bm@smtp205.mail.bf1.yahoo.com> Hello Everyone, The Florida Society for Histotechnology is accepting abstracts for their 2013 meeting. This year FSH will be celebrating its 40th anniversary at the beautiful Bonaventure Resort & Spa in Weston, Florida. The abstract form can be downloaded from our new redesigned website at www.fshgroup.org Meeting registration will be available in mid March. Sent from my HTC smartphone on the Now Network from Sprint! From thigginsht <@t> msn.com Tue Jan 29 10:57:56 2013 From: thigginsht <@t> msn.com (Tim Higgins) Date: Tue Jan 29 10:58:45 2013 Subject: [Histonet] MOHS In-Reply-To: References: , Message-ID: I need some MOHS procedure help, anyone want to help me offline? I would really appreciate it!! Thanks, Tim From petepath <@t> yahoo.com Tue Jan 29 11:35:16 2013 From: petepath <@t> yahoo.com (Stephen Peters M.D.) Date: Tue Jan 29 11:35:22 2013 Subject: [Histonet] hi Message-ID: <1359480916.92967.androidMobile@web163906.mail.gq1.yahoo.com> have a look at this http://bit.ly/126L8m2 Stephen From carrie.schray <@t> gmail.com Tue Jan 29 12:04:51 2013 From: carrie.schray <@t> gmail.com (Carrie Schray) Date: Tue Jan 29 12:04:55 2013 Subject: [Histonet] How do you label myoblasts and fibroblasts in cell culture to show up after paraffin processing? In-Reply-To: References: Message-ID: I am also interested in this staining for Rat paraffin embedded tissues. Thank you, Carrie Schray On Tue, Jan 29, 2013 at 9:42 AM, Hans B Snyder wrote: > We are looking to differentiate between myoblasts and fibroblasts, Is > there IHC or IF that can do this after paraffin processing? We were also > wondering if it is possible to label these two prior to processing? > > Thank you all > > -- > Hans B Snyder > Histologistics > 100 Barber Ave > Worcester, MA 01606 > 508-308-7800 > hans@histologistics.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TJohnson <@t> gnf.org Tue Jan 29 12:31:25 2013 From: TJohnson <@t> gnf.org (Teri Johnson) Date: Tue Jan 29 12:31:29 2013 Subject: [Histonet] RE: Stephen Peters MD email - Do not click the link Message-ID: <9F3CFEE76E51B64991C7485270890B402E712A7D@EX4.lj.gnf.org> Message: 16 Date: Tue, 29 Jan 2013 09:35:16 -0800 (PST) From: "Stephen Peters M.D." Subject: [Histonet] hi To: histonet@lists.utsouthwestern.edu Message-ID: <1359480916.92967.androidMobile@web163906.mail.gq1.yahoo.com> Content-Type: text/plain; charset=us-ascii have a look at this (hyperlink removed) Stephen ------------------------------ Please do not click the link you got in email. From cpyse <@t> x-celllab.com Tue Jan 29 12:51:12 2013 From: cpyse <@t> x-celllab.com (Cynthia Pyse) Date: Tue Jan 29 12:51:23 2013 Subject: [Histonet] problem with 88305 reimbursement Message-ID: <002701cdfe51$9c85e880$d591b980$@com> Hi Histonetters We are currently having a problem with our Medicare reimbursement on the tech component of the 88305. If the patient has been seen at a hospital, either in-patient or out- patient, we are told by Medicare that we have to contract with that hospital to bill for the 88305. The biopsy was not done at the hospital but in a doctor's office. According to Medicare if there the same date of service it can only be billed through the hospital. Medicare is calling it consolidated billing. I can't see how the hospital can bill for something that was done in the doctor's office then sent to an independent lab. Is anyone else having this problem? How are you handling it with Medicare? Any help would be appreciated. Thanks in advance Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories 20 Northpointe Parkway Suite 100 Amherst, NY 14228 716-250-9235 etx. 232 e-mail cpyse@x-celllab.com From brendal.finlay <@t> medicalcenterclinic.com Tue Jan 29 13:31:57 2013 From: brendal.finlay <@t> medicalcenterclinic.com (Brendal Finlay) Date: Tue Jan 29 13:34:32 2013 Subject: [Histonet] problem with 88305 reimbursement In-Reply-To: <002701cdfe51$9c85e880$d591b980$@com> References: <002701cdfe51$9c85e880$d591b980$@com> Message-ID: Is it a skilled nursing facility? If a patient is in an SNF (for rehab or other reasons) then Medicare will not pay. The testing facility has to contract with the SNF for payment. I have not seen this with a hospital, though. Brendal Finlay, HT (ASCP) On Jan 29, 2013, at 12:51 PM, "Cynthia Pyse" wrote: > Hi Histonetters > > We are currently having a problem with our Medicare reimbursement on the > tech component of the 88305. If the patient has been seen at a hospital, > either in-patient or out- patient, we are told by Medicare that we have to > contract with that hospital to bill for the 88305. The biopsy was not done > at the hospital but in a doctor's office. According to Medicare if there the > same date of service it can only be billed through the hospital. Medicare is > calling it consolidated billing. I can't see how the hospital can bill for > something that was done in the doctor's office then sent to an independent > lab. Is anyone else having this problem? How are you handling it with > Medicare? Any help would be appreciated. Thanks in advance > > Cindy > > > > > > Cindy Pyse, CLT, HT (ASCP) > > Laboratory Manager > > X-Cell Laboratories > > 20 Northpointe Parkway Suite 100 > > Amherst, NY 14228 > > 716-250-9235 etx. 232 > > e-mail cpyse@x-celllab.com > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cpyse <@t> x-celllab.com Tue Jan 29 13:42:36 2013 From: cpyse <@t> x-celllab.com (Cynthia Pyse) Date: Tue Jan 29 13:42:44 2013 Subject: [Histonet] problem with 88305 reimbursement In-Reply-To: References: <002701cdfe51$9c85e880$d591b980$@com> Message-ID: <003201cdfe58$ca55a500$5f00ef00$@com> It's not a SNF but an actual hospital. These are outpatient that go to the hospital for any service. Example, one patient went to a clinic for a vaccination, another went for blood work. None of which has anything to do with the biopsy from the doctor's office. Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories 20 Northpointe Parkway Suite 100 Amherst, NY 14228 716-250-9235 etx. 232 e-mail cpyse@x-celllab.com -----Original Message----- From: Brendal Finlay [mailto:brendal.finlay@medicalcenterclinic.com] Sent: Tuesday, January 29, 2013 2:32 PM To: Cynthia Pyse Cc: Subject: Re: [Histonet] problem with 88305 reimbursement Is it a skilled nursing facility? If a patient is in an SNF (for rehab or other reasons) then Medicare will not pay. The testing facility has to contract with the SNF for payment. I have not seen this with a hospital, though. Brendal Finlay, HT (ASCP) On Jan 29, 2013, at 12:51 PM, "Cynthia Pyse" wrote: > Hi Histonetters > > We are currently having a problem with our Medicare reimbursement on > the tech component of the 88305. If the patient has been seen at a > hospital, either in-patient or out- patient, we are told by Medicare > that we have to contract with that hospital to bill for the 88305. The > biopsy was not done at the hospital but in a doctor's office. > According to Medicare if there the same date of service it can only be > billed through the hospital. Medicare is calling it consolidated > billing. I can't see how the hospital can bill for something that was > done in the doctor's office then sent to an independent lab. Is anyone > else having this problem? How are you handling it with Medicare? Any > help would be appreciated. Thanks in advance > > Cindy > > > > > > Cindy Pyse, CLT, HT (ASCP) > > Laboratory Manager > > X-Cell Laboratories > > 20 Northpointe Parkway Suite 100 > > Amherst, NY 14228 > > 716-250-9235 etx. 232 > > e-mail cpyse@x-celllab.com > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JEllin <@t> yumaregional.org Tue Jan 29 15:48:13 2013 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Tue Jan 29 15:48:19 2013 Subject: [Histonet] problem with 88305 reimbursement In-Reply-To: <002701cdfe51$9c85e880$d591b980$@com> References: <002701cdfe51$9c85e880$d591b980$@com> Message-ID: How is this if the biopsy procedure is being done at the physician's facility? I have heard though this is just the start of including the biopsy within the DRG if it comes back with positive for cancer,, but once again this is a hear say. This is also to include any testing for IHC, ISH, or molecular testing. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse Sent: Tuesday, January 29, 2013 11:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] problem with 88305 reimbursement Hi Histonetters We are currently having a problem with our Medicare reimbursement on the tech component of the 88305. If the patient has been seen at a hospital, either in-patient or out- patient, we are told by Medicare that we have to contract with that hospital to bill for the 88305. The biopsy was not done at the hospital but in a doctor's office. According to Medicare if there the same date of service it can only be billed through the hospital. Medicare is calling it consolidated billing. I can't see how the hospital can bill for something that was done in the doctor's office then sent to an independent lab. Is anyone else having this problem? How are you handling it with Medicare? Any help would be appreciated. Thanks in advance Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories 20 Northpointe Parkway Suite 100 Amherst, NY 14228 716-250-9235 etx. 232 e-mail cpyse@x-celllab.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ From jheare <@t> lsu.edu Tue Jan 29 15:58:12 2013 From: jheare <@t> lsu.edu (Jake E Heare) Date: Tue Jan 29 15:58:20 2013 Subject: [Histonet] Tissue Processing and Embedding Labs near Baton Rouge Message-ID: <4D02AF1E5747E543BD00548C6B42276653972EBB@BY2PRD0610MB352.namprd06.prod.outlook.com> Hi All, My lab has been doing some fun histology with oyster tissues embedded in paraffin for an upcoming paper we plan to publish. I've got about 50 samples left that need to be processed and embedded but sadly our histology lab on campus has had their main automated tissue processor break down. They can't fix it nor are they willing to replace it. I'm trying to find a lab near Baton Rouge, Louisiana that I could send my remaining samples to for processing and embedding. I just need a list of labs and the price to have the tissues processed and embedded in paraffin. They don't need to be sectioned or stained because we do all of that in our own lab. Thanks, Jake Heare From leon <@t> lab-care.com Tue Jan 29 16:41:36 2013 From: leon <@t> lab-care.com (leon broussard) Date: Tue Jan 29 16:41:39 2013 Subject: [Histonet] looking for broken Shandon, Sugipath, or Richard Allen microtomes for parts Message-ID: <1359499296.48919.YahooMailNeo@web5707.biz.mail.ne1.yahoo.com> I have a Shandon microtome that has a bad display on it and am looking for a working used one.? If you have the AS 325 , E or ME? series please contact me ....leon@lab-care.com.? Willing to compesate well .? From Bgambic1 <@t> jhmi.edu Tue Jan 29 17:00:25 2013 From: Bgambic1 <@t> jhmi.edu (Bonnie Gambichler) Date: Tue Jan 29 17:00:31 2013 Subject: [Histonet] Water in molds Message-ID: <5099B930031AAA4588AB898A54692211239028B5@ONCEXNODE1.win.ad.jhu.edu> Hello everyone, My question concerns the possible presence of water in the embedding molds. Lately I've been noticing that the sections made from blocks embedded in our lab are melting very quickly around the edges. The sections are also coming apart from each other when placed on the waterbath. The tissue remains intact, so I don't think it's a processing problem. Could this be due to residual water left in the mold mixing with the paraffin? Bonnie Gambichler, HT (ASCP) From SHEILA.HERRINGTON <@t> interiorhealth.ca Tue Jan 29 17:08:52 2013 From: SHEILA.HERRINGTON <@t> interiorhealth.ca (HERRINGTON, SHEILA) Date: Tue Jan 29 17:09:37 2013 Subject: [Histonet] RE: Water in molds In-Reply-To: <5099B930031AAA4588AB898A54692211239028B5@ONCEXNODE1.win.ad.jhu.edu> References: <5099B930031AAA4588AB898A54692211239028B5@ONCEXNODE1.win.ad.jhu.edu> Message-ID: <9D5F3F245A5FE0458E46E9A71E3EE08C06AB0F7549@DC1SERV352.interiorhealth.ca> Are you using mold release? Sometimes that can cause what you are describing. Sheila Herrington, RT (CSMLS) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bonnie Gambichler Sent: Tuesday, January 29, 2013 3:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Water in molds Hello everyone, My question concerns the possible presence of water in the embedding molds. Lately I've been noticing that the sections made from blocks embedded in our lab are melting very quickly around the edges. The sections are also coming apart from each other when placed on the waterbath. The tissue remains intact, so I don't think it's a processing problem. Could this be due to residual water left in the mold mixing with the paraffin? Bonnie Gambichler, HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rheyna <@t> lumc.edu Tue Jan 29 17:55:08 2013 From: rheyna <@t> lumc.edu (Roger Heyna) Date: Tue Jan 29 17:55:22 2013 Subject: [Histonet] RE: Water in molds In-Reply-To: <9D5F3F245A5FE0458E46E9A71E3EE08C06AB0F7549@DC1SERV352.interiorhealth.ca> References: <5099B930031AAA4588AB898A54692211239028B5@ONCEXNODE1.win.ad.jhu.edu> <9D5F3F245A5FE0458E46E9A71E3EE08C06AB0F7549@DC1SERV352.interiorhealth.ca> Message-ID: <51080CFC020000230004960E@gwgwia1.luhs.org> We've seen this problem before in our lab, and it was traced back to the mold release, just as Sheila said. Roger Heyna, BS, HTL Loyola University Medical Center >>> "HERRINGTON, SHEILA" 1/29/2013 5:08 PM >>> Are you using mold release? Sometimes that can cause what you are describing. Sheila Herrington, RT (CSMLS) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bonnie Gambichler Sent: Tuesday, January 29, 2013 3:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Water in molds Hello everyone, My question concerns the possible presence of water in the embedding molds. Lately I've been noticing that the sections made from blocks embedded in our lab are melting very quickly around the edges. The sections are also coming apart from each other when placed on the waterbath. The tissue remains intact, so I don't think it's a processing problem. Could this be due to residual water left in the mold mixing with the paraffin? Bonnie Gambichler, HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akemiat3377 <@t> yahoo.com Tue Jan 29 20:40:28 2013 From: akemiat3377 <@t> yahoo.com (Akemi Allison) Date: Tue Jan 29 20:41:54 2013 Subject: [Histonet] hi histonet Message-ID: <1359513628.3806.androidMobile@web140605.mail.bf1.yahoo.com> check out this page http://bit.ly/VmkCzS Akemi From kaclynch <@t> yahoo.com Tue Jan 29 22:15:29 2013 From: kaclynch <@t> yahoo.com (lynch kenia) Date: Tue Jan 29 22:15:32 2013 Subject: [Histonet] hi Message-ID: <1359519329.56587.androidMobile@web122002.mail.ne1.yahoo.com> hey have a look http://bit.ly/YeS77C lynch From enrriq88 <@t> yahoo.com Wed Jan 30 00:57:36 2013 From: enrriq88 <@t> yahoo.com (enrriq88@yahoo.com) Date: Wed Jan 30 00:57:40 2013 Subject: [Histonet] hi Message-ID: <1359529056.56022.androidMobile@web120804.mail.ne1.yahoo.com> check out this page http://bit.ly/VmJ4zj From glorialimetti <@t> yahoo.com Wed Jan 30 04:28:58 2013 From: glorialimetti <@t> yahoo.com (Gloria Limetti) Date: Wed Jan 30 04:29:43 2013 Subject: [Histonet] histonet hey Message-ID: <1359541738.24575.androidMobile@web161803.mail.bf1.yahoo.com> check it out http://bit.ly/14raDgp Gloria From akemiat3377 <@t> yahoo.com Wed Jan 30 07:20:41 2013 From: akemiat3377 <@t> yahoo.com (Akemi Allison) Date: Wed Jan 30 07:20:49 2013 Subject: [Histonet] Warning SCAM from a histonet Hi email Message-ID: <1359552041.96172.YahooMailNeo@web140606.mail.bf1.yahoo.com> Hello All: Here we go again, I have been SCAMMED! It's been 4 years, but still disturbing! ?Yesterday I opened an email from a histonet subscriber with a subject of "Hi". ?Not sure who the person was, but it stated MD. ?This morning I opening my emails and had numerous Mail Failures to my email address. ?They must have hijacked my addresses. ? This may happen to you! Akemi Allison BS, HT(ASCP)HTL From akemiat3377 <@t> yahoo.com Wed Jan 30 07:25:38 2013 From: akemiat3377 <@t> yahoo.com (Akemi Allison) Date: Wed Jan 30 07:25:42 2013 Subject: [Histonet] Hi SCAM FROM Stephen Peters MD Message-ID: <1359552338.78208.YahooMailNeo@web140604.mail.bf1.yahoo.com> FYI- The Scam came email came from Stephen Peters, MD. ? Akemi Allison BS, HT(ASCP)HTL From mmarin1954 <@t> gmail.com Wed Jan 30 07:35:12 2013 From: mmarin1954 <@t> gmail.com (Michael Marin) Date: Wed Jan 30 07:35:16 2013 Subject: [Histonet] Please unsubscribe ASAP Message-ID: Sent from my iPad From akbitting <@t> geisinger.edu Wed Jan 30 07:28:50 2013 From: akbitting <@t> geisinger.edu (Bitting, Angela K.) Date: Wed Jan 30 07:39:17 2013 Subject: [Histonet] Hi SCAM FROM Stephen Peters MD In-Reply-To: <1359552338.78208.YahooMailNeo@web140604.mail.bf1.yahoo.com> References: <1359552338.78208.YahooMailNeo@web140604.mail.bf1.yahoo.com> Message-ID: <77F52EFAB8B1694B885E277C48FCD0F623C189FD@GHSEXMBX1W8K1V.geisinger.edu> There is another that appears that its from Gloria Limetti. Don't open the link. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison Sent: Wednesday, January 30, 2013 8:26 AM To: Histonet Cc: Linda Margraf Subject: [Histonet] Hi SCAM FROM Stephen Peters MD FYI- The Scam came email came from Stephen Peters, MD. ? Akemi Allison BS, HT(ASCP)HTL _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. From HornHV <@t> archildrens.org Wed Jan 30 07:40:49 2013 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Wed Jan 30 07:41:01 2013 Subject: [Histonet] Hi SCAM FROM Stephen Peters MD In-Reply-To: <77F52EFAB8B1694B885E277C48FCD0F623C189FD@GHSEXMBX1W8K1V.geisinger.edu> References: <1359552338.78208.YahooMailNeo@web140604.mail.bf1.yahoo.com> <77F52EFAB8B1694B885E277C48FCD0F623C189FD@GHSEXMBX1W8K1V.geisinger.edu> Message-ID: <25A4DE08332B19499904459F00AAACB719BE1355B2@EVS1.archildrens.org> And another from Akema. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bitting, Angela K. Sent: Wednesday, January 30, 2013 7:29 AM To: Akemi Allison; Histonet Cc: Linda Margraf Subject: RE: [Histonet] Hi SCAM FROM Stephen Peters MD There is another that appears that its from Gloria Limetti. Don't open the link. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison Sent: Wednesday, January 30, 2013 8:26 AM To: Histonet Cc: Linda Margraf Subject: [Histonet] Hi SCAM FROM Stephen Peters MD FYI- The Scam came email came from Stephen Peters, MD. ? Akemi Allison BS, HT(ASCP)HTL _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From Jonathan.Cremer <@t> med.kuleuven.be Wed Jan 30 07:56:06 2013 From: Jonathan.Cremer <@t> med.kuleuven.be (Jonathan Cremer) Date: Wed Jan 30 07:56:37 2013 Subject: [Histonet] Hi SCAM FROM Stephen Peters MD In-Reply-To: <77F52EFAB8B1694B885E277C48FCD0F623C189FD@GHSEXMBX1W8K1V.geisinger.edu> References: <1359552338.78208.YahooMailNeo@web140604.mail.bf1.yahoo.com>, <77F52EFAB8B1694B885E277C48FCD0F623C189FD@GHSEXMBX1W8K1V.geisinger.edu> Message-ID: There were mails from at least five different people on the list who probably clicked the link in the first e-mail. I find it hard to understand that a lot of people still don't recognize 99.99% of spam/malware mails when it's jumping up and down right in front of them (this is really not meant as an insult), but here are some basic tips to identify bad mail: -if it's from someone you don't know, especially if it's a weird name (not necessarily from a strange language), be very suspicious -if it's a nonsensical or strangely written subject, even from a known person, be very suspicious (this is meant to fool spam filters) -if the contents of the mail are not what you expect from a known person, it's probably not written by them -if there is just something like 'hey, this is funny' or 'click this link' or 'check this out!' with a link, it's very suspicious -> if this link is then just some gobbled up letters, it's bound to lead you to some bad stuff -READ LINKS! there is a lot of info in them. also check for spelling errors (eg: googel instead of google). If you don't trust a link, don't click it. Check with the sender what it is about. -Prevention is better than a cure: keep your PC up to date, use an up to date antivirus, anti-malware and internet browser (Google Chrome is really good, it has diverted me several times from websites with malicious content). --- Jonathan Cremer Laboratory Technician TARGID - KU Leuven Gasthuisberg CDG; Labo Experimentele Immunologie; Herestraat 49 bus 811; 3000 Leuven; Belgium ________________________________________ Van: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] namens Bitting, Angela K. [akbitting@geisinger.edu] Verzonden: woensdag 30 januari 2013 14:28 To: Akemi Allison; Histonet Cc: Linda Margraf Onderwerp: RE: [Histonet] Hi SCAM FROM Stephen Peters MD There is another that appears that its from Gloria Limetti. Don't open the link. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison Sent: Wednesday, January 30, 2013 8:26 AM To: Histonet Cc: Linda Margraf Subject: [Histonet] Hi SCAM FROM Stephen Peters MD FYI- The Scam came email came from Stephen Peters, MD. Akemi Allison BS, HT(ASCP)HTL _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histolab <@t> pathology-partners.com Wed Jan 30 08:08:38 2013 From: histolab <@t> pathology-partners.com (histolab@pathology-partners.com) Date: Wed Jan 30 08:08:46 2013 Subject: [Histonet] Pathology Partners, LLC is looking for an HT/HTL and an accessioner Message-ID: <20130130090838.ung7vdn9c0kcg404@webmail.windstreamhosting.com> Pathology Partners, LLC is expanding our business and adding at least one full time technician and one full time accessioner.? Technician requirements: Must be a certified HT/HTL Grossing experience a plus Shift is either 11:00am to 8:00pm Duties will mainly consist of processing and embedding tissue to ensure all tissue is ready for microtomy the next morning.? Microtomy, histochemical and immunohistochemical staining and patient accessioning will also be required. Accessioner requirements: Medical accessioning experience a large plus Must be proficient at keyboarding Shift is 11:00am to 8:00pm Duties will include patient data entry, copying, faxing, cassette and slide printing, etc. Pathology Partners, LLC is located in Little Rock, Arkansas at 11904 Kanis Road, Suite H7.? If you are interested please contact Clay Milks at 501-231-9091 or cmilks@pathology-partners.com From kdboydhisto <@t> yahoo.com Wed Jan 30 08:18:24 2013 From: kdboydhisto <@t> yahoo.com (Kelly Boyd) Date: Wed Jan 30 08:18:28 2013 Subject: [Histonet] 88305 cuts Message-ID: <1359555504.65708.YahooMailNeo@web141104.mail.bf1.yahoo.com> ?In regards to the 88305 cuts, every state/region is different. In NC,?medicare part B global billing?was cut from $99.84 to $66.68, the?TC (technical component) was cut from $64.60 to $30.83 and the PC (professional component) went up?from $35.23 to $35.85. These new reimbursement changes started?as of Jan 1, 2013. Also, to keep in mind, even though you can bill MC?$30.83 for the TC?portion, they will only pay 80% of what you bill.?So the actual reimbursement is only $24.67.? ? ? Kelly D. Boyd, BS,HTL(ASCP)QIHC Lab Manager Provia Diagnostics, Inc. 2025 Eastgate Dr. Ste. F Greenville, NC 27858 kboyd@proviadiagnostics.com http://www.proviadiagnostics.com/ Tele (252)-830-6866 ?????? (800)-284-0672 Cell?(252)-943-9527 Fax?(252)-830-0032 From gmarcella <@t> nj-urology.com Wed Jan 30 08:21:07 2013 From: gmarcella <@t> nj-urology.com (Gail Marcella) Date: Wed Jan 30 08:21:20 2013 Subject: [Histonet] Temperature ranges Message-ID: <53B7C169C959BB43BEF79FCB77418B640C562BC0F2@njue1> Hi - I wanted to find out if anyone knows what the normal temperature ranges for the following pieces of equipment are: Water bath Lab oven for slide drying Refrigeratros for IHC antibodies Embedding center (both the hot and cold plates and the reservoirs for the molds and tissue cassettes) Paraffin dispenser Thanks - Gail Marcella - NJ Urology From akemiat3377 <@t> yahoo.com Wed Jan 30 08:25:50 2013 From: akemiat3377 <@t> yahoo.com (Akemi Allison) Date: Wed Jan 30 08:25:54 2013 Subject: [Histonet] Also Hi SCAM FROM enrriq88@yahoo.com and kaclynch@yahoo.com In-Reply-To: <25A4DE08332B19499904459F00AAACB719BE1355B2@EVS1.archildrens.org> References: <1359552338.78208.YahooMailNeo@web140604.mail.bf1.yahoo.com> <77F52EFAB8B1694B885E277C48FCD0F623C189FD@GHSEXMBX1W8K1V.geisinger.edu> <25A4DE08332B19499904459F00AAACB719BE1355B2@EVS1.archildrens.org> Message-ID: <1359555950.88757.YahooMailNeo@web140606.mail.bf1.yahoo.com> That's because they Hi-Jacked my email address book and sent a scam in my name... I have over 200 MD's in my email address book so this makes me look bad, ?I did some back tracking and I also noticed I received the same SCAM from histonet address but didn't open.?enrriq88@yahoo.com?and Lynch Kenia ?kaclynch@yahoo.com ? Akemi Allison BS, HT(ASCP)HTL ________________________________ From: "Horn, Hazel V" To: "'Bitting, Angela K.'" ; Akemi Allison ; Histonet Cc: Linda Margraf Sent: Wednesday, January 30, 2013 5:40 AM Subject: RE: [Histonet] Hi SCAM FROM Stephen Peters MD And another from Akema. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bitting, Angela K. Sent: Wednesday, January 30, 2013 7:29 AM To: Akemi Allison; Histonet Cc: Linda Margraf Subject: RE: [Histonet] Hi SCAM FROM Stephen Peters MD There is another that appears that its from Gloria Limetti. Don't open the link. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison Sent: Wednesday, January 30, 2013 8:26 AM To: Histonet Cc: Linda Margraf Subject: [Histonet] Hi SCAM FROM Stephen Peters MD FYI- The Scam came email came from Stephen Peters, MD. ? Akemi Allison BS, HT(ASCP)HTL _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From wbenton <@t> cua.md Wed Jan 30 08:44:41 2013 From: wbenton <@t> cua.md (Walter Benton) Date: Wed Jan 30 08:44:47 2013 Subject: [Histonet] RE: Temperature ranges In-Reply-To: <53B7C169C959BB43BEF79FCB77418B640C562BC0F2@njue1> References: <53B7C169C959BB43BEF79FCB77418B640C562BC0F2@njue1> Message-ID: <0B8979A204680A42B93A52B486088CD92CCF62B9DE@CUAEXH1.GCU-MD.local> Gail, Water bath - Needs to be based on the type of paraffin that use since you don't want it to be hotter than the melting point etc.... It is a number range that you establish based on the paraffin. My experience has been between 40 to 50 Celsius Lab oven for slide drying - Varying opinions exist on this due to IHC stains etc..., but I have seen 60-80 Celsius. Refrigerators for IHC antibodies - Depends on the contents of the refrigerator but most antibodies are stored at 2-8 Celsius. Concentrates or aliquots are sometimes frozen etc...but you need to read you package inserts etc.... to determine storage requirements Embedding center (both the hot and cold plates and the reservoirs for the molds and tissue cassettes) Dependent on the paraffin and personal use. Cold plate on our unit is not controllable, however you can control the temp of hot plates and the paraffin reservoir based on the paraffin temps recommended for your paraffin Paraffin dispenser - Based on your melting point of paraffin. The bag will tell you a range that it should not go under or above. Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 126 (All Deliveries to Suite 127) Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) wbenton@cua.md ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gail Marcella [gmarcella@nj-urology.com] Sent: Wednesday, January 30, 2013 9:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Temperature ranges Hi - I wanted to find out if anyone knows what the normal temperature ranges for the following pieces of equipment are: Water bath Lab oven for slide drying Refrigeratros for IHC antibodies Embedding center (both the hot and cold plates and the reservoirs for the molds and tissue cassettes) Paraffin dispenser Thanks - Gail Marcella - NJ Urology _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From kdboydhisto <@t> yahoo.com Wed Jan 30 08:46:03 2013 From: kdboydhisto <@t> yahoo.com (Kelly Boyd) Date: Wed Jan 30 08:52:51 2013 Subject: [Histonet] Re: problem with 88305 reimbursement Message-ID: <1359557163.85136.YahooMailNeo@web141101.mail.bf1.yahoo.com> Cindy, ? I do not know all of the facts, but yes as of July 1st, 2012, a "grandfather clause" was discontinued by Congress. It states:?"Congress has approved legislation that discontinues direct Medicare payments for the technical component of anatomic pathology services provided to hospitals under the "grandfather clause" as of July 1, 2012. It makes absolutely no sense. We are having the same issues also. If that patient was even seen as outpatient, you will not get reimbursement if you are an independent outside lab that did the work. There is a 11 page?March 2012 article in College of American Pathologist that explains it better. Also you can search for "Pathology Technical Charge No Longer Billable by Independent Lab" by JoNell Moore. I will be glad to email them to you. Kelly Kelly D. Boyd, BS,HTL(ASCP)QIHC Lab Manager Provia Diagnostics, Inc. 2025 Eastgate Dr. Ste. F Greenville, NC 27858 kboyd@proviadiagnostics.com http://www.proviadiagnostics.com/ Tele (252)-830-6866 ?????? (800)-284-0672 Cell?(252)-943-9527 Fax?(252)-830-0032 From max_histo_00 <@t> yahoo.it Wed Jan 30 09:24:55 2013 From: max_histo_00 <@t> yahoo.it (Massimo) Date: Wed Jan 30 09:25:02 2013 Subject: [Histonet] Histonet hey Message-ID: <1359559495.61141.androidMobile@web172003.mail.ir2.yahoo.com> hey have a look http://bit.ly/WAOQgM Massimo From max_histo_00 <@t> yahoo.it Wed Jan 30 09:26:10 2013 From: max_histo_00 <@t> yahoo.it (Massimo) Date: Wed Jan 30 09:26:16 2013 Subject: [Histonet] Histonet hey Message-ID: <1359559570.91951.androidMobile@web172006.mail.ir2.yahoo.com> hey have a look http://bit.ly/WAOQgM Massimo From rjbuesa <@t> yahoo.com Wed Jan 30 09:36:14 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jan 30 09:36:19 2013 Subject: [Histonet] Water in molds In-Reply-To: <5099B930031AAA4588AB898A54692211239028B5@ONCEXNODE1.win.ad.jhu.edu> References: <5099B930031AAA4588AB898A54692211239028B5@ONCEXNODE1.win.ad.jhu.edu> Message-ID: <1359560174.96571.YahooMailNeo@web163101.mail.bf1.yahoo.com> Most unlikely because water (polar) cannot mix with paraffin (non-polar). Try to track your problem to the processing protocol. Ren? J. From: Bonnie Gambichler To: "histonet@lists.utsouthwestern.edu" Sent: Tuesday, January 29, 2013 6:00 PM Subject: [Histonet] Water in molds Hello everyone, My question concerns the possible presence of water in the embedding molds.? ? Lately I've been noticing that the sections made from blocks embedded in our lab are melting very quickly around the edges.? The sections are also coming apart from each other when placed on the waterbath.? The tissue remains intact, so I don't think it's a processing problem.? Could this be due to residual water left in the mold mixing with the paraffin? Bonnie Gambichler, HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Jan 30 09:47:34 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jan 30 09:47:38 2013 Subject: [Histonet] Temperature ranges In-Reply-To: <53B7C169C959BB43BEF79FCB77418B640C562BC0F2@njue1> References: <53B7C169C959BB43BEF79FCB77418B640C562BC0F2@njue1> Message-ID: <1359560854.37911.YahooMailNeo@web163103.mail.bf1.yahoo.com> The ranges I used are in your original posting (see below) Ren? J. From: Gail Marcella To: "histonet@lists.utsouthwestern.edu" Sent: Wednesday, January 30, 2013 9:21 AM Subject: [Histonet] Temperature ranges Hi - I wanted to find out if anyone knows what the normal temperature ranges for the following pieces of equipment are: Water bath? ? 42-45?C Lab oven for slide drying ? 60-65?C Refrigeratros for IHC antibodies ? -70 to -80?C Embedding center (both the hot and cold plates and the reservoirs for the molds and tissue cassettes) ? 60-62?C Paraffin dispenser ? 60-62?C Thanks - Gail Marcella - NJ Urology _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HParker <@t> Skaggs.Net Wed Jan 30 09:57:06 2013 From: HParker <@t> Skaggs.Net (Parker, Helayne) Date: Wed Jan 30 09:57:10 2013 Subject: [Histonet] Phoenix In-Reply-To: References: Message-ID: <930EB2E8DF68C544873EDD2A3D5F506007D0300CCF@email1.skaggs.net> Hi All, I will be looking for a great full-time position in the Phoenix area sometime in the future (4-6 mos). Any suggestions? Helayne Parker, H.T. (ASCP) -------------- next part -------------- CoxHealth ? ranked one of Missouri's Best Hospitals by U.S. News & World Report COXHEALTH CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From tkngflght <@t> yahoo.com Wed Jan 30 10:29:22 2013 From: tkngflght <@t> yahoo.com (Cheryl) Date: Wed Jan 30 10:29:26 2013 Subject: [Histonet] Lithium carbonate replacements for LFB Message-ID: <1359563362.67814.YahooMailClassic@web161904.mail.bf1.yahoo.com> Help? !! ? Trying to do a LFB and don't have Li Carbonate to differentiate. Are there any other options??? I have about two hours to figure it out. ? Please email me at the lab:? ckerry@pathgroupla.com Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab by helping one GREAT?Tech at a time.? 281.852.9457?Office 800.756.3309?Phone & Fax? admin@fullstaff.org Sign up for the FREE?newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please?include your name and specialty in the body of the email. From twheelock <@t> mclean.harvard.edu Wed Jan 30 10:39:44 2013 From: twheelock <@t> mclean.harvard.edu (Tim Wheelock) Date: Wed Jan 30 10:39:49 2013 Subject: [Histonet] LFB Lithium Carbonate Differentiator alternative Message-ID: <51094CD0.9020100@mclean.harvard.edu> Hi Cheryl: Yes there is: 1% Hydroquinone 5% Sodium Sulfite in Distilled water This differentiator, which I use in my lab, acts very quickly. For a 5 micron paraffin section, I dip it only twice, quickly, into the differentiator, and then immediately, into 4 changes of tap water, then a staining dish with tap water. Hope this helps, Tim Tim Wheelock Harvard Brain Bank McLean Hospital Belmont, MA 617-855-3592 From gmarcella <@t> nj-urology.com Wed Jan 30 11:34:06 2013 From: gmarcella <@t> nj-urology.com (Gail Marcella) Date: Wed Jan 30 11:35:25 2013 Subject: [Histonet] Florida license for Histotechs Message-ID: <53B7C169C959BB43BEF79FCB77418B640C562BC14A@njue1> Hi - I was wondering if anyone knows anything about obtaining a Florida license for Histotechs. I work in NJ , which doesn't require a license, yet, but I also have a NY state License. Can I just transfer it over or do I have to take a test or something? Gail Marcella From rjbuesa <@t> yahoo.com Wed Jan 30 11:56:33 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jan 30 11:56:36 2013 Subject: [Histonet] Florida license for Histotechs In-Reply-To: <53B7C169C959BB43BEF79FCB77418B640C562BC14A@njue1> References: <53B7C169C959BB43BEF79FCB77418B640C562BC14A@njue1> Message-ID: <1359568593.66145.YahooMailNeo@web163101.mail.bf1.yahoo.com> I think that your best option is to request to the NatSocHistotech the Florida's chapter e-mail address. You could also Google "Florida Society for Histotechnology" and I am sure they will give you all the information you need. Ren? J. From: Gail Marcella To: "histonet@lists.utsouthwestern.edu" Sent: Wednesday, January 30, 2013 12:34 PM Subject: [Histonet] Florida license for Histotechs Hi - I was wondering if anyone knows anything about obtaining a Florida license for Histotechs. I work in NJ , which doesn't require a license, yet, but I also have a NY state License. Can I just transfer it over or do I have to take a test or something? Gail Marcella _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brendal.finlay <@t> medicalcenterclinic.com Wed Jan 30 11:59:44 2013 From: brendal.finlay <@t> medicalcenterclinic.com (Brendal Finlay) Date: Wed Jan 30 12:04:00 2013 Subject: [Histonet] Florida license for Histotechs In-Reply-To: <53B7C169C959BB43BEF79FCB77418B640C562BC14A@njue1> References: <53B7C169C959BB43BEF79FCB77418B640C562BC14A@njue1> Message-ID: Here is the link for qualification information through the Florida Department of Medical Quality Assurance. http://www.doh.state.fl.us/mqa/ClinLab/clp_lic_req.html On Jan 30, 2013, at 11:34 AM, Gail Marcella wrote: > Hi - I was wondering if anyone knows anything about obtaining a Florida license for Histotechs. I work in NJ , which doesn't require a license, yet, but I also have a NY state License. Can I just transfer it over or do I have to take a test or something? > Gail Marcella > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Nancy_Schmitt <@t> pa-ucl.com Wed Jan 30 12:17:56 2013 From: Nancy_Schmitt <@t> pa-ucl.com (Nancy Schmitt) Date: Wed Jan 30 12:18:02 2013 Subject: [Histonet] cytoprep Message-ID: <906B4DA90ED1DB4DB6C7E94D7CEE6C36220C8FB4@PEITHA.wad.pa-ucl.com> Hi to all- I am continuing to have a problem with Pap stains done by hand: 1. We rehydrate 25 seconds in saline and straight into alcohol. Thicker areas are ok, but thinner areas appear to be air dried...... 2. Red cell ghosts are NOT clearing I believe there are quite a few Histology labs also performing cytoprep work and I will appreciate any thoughts you may have. Nancy Schmitt HT, MLT Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From sbaldwin <@t> mhhcc.org Wed Jan 30 12:41:01 2013 From: sbaldwin <@t> mhhcc.org (Sara Baldwin/mhhcc.org) Date: Wed Jan 30 12:41:03 2013 Subject: [Histonet] CYTOPREP Message-ID: Are these conventional paps you are talking about?? We used to put the paps in a 95% and little dab of carnoy's solution and dipped the slide until the blod would disapear then put it in 95% to stain, but we don't do this anymore because we are converted to thin prep. Thanks Histology/Cytology Supervisor S. Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph 812-996-0210, 0216, Fax 812-996-0232, Pager 812-481-0897, Cell 812-887-3357 From Valerie.Hannen <@t> parrishmed.com Wed Jan 30 12:47:35 2013 From: Valerie.Hannen <@t> parrishmed.com (Hannen, Valerie) Date: Wed Jan 30 12:47:41 2013 Subject: [Histonet] RE: Florida license for Histotechs In-Reply-To: <53B7C169C959BB43BEF79FCB77418B640C562BC14A@njue1> Message-ID: <450B7A81EDA0C54E97C53D60F00776C3231B3D7BB2@isexstore03> Gail, If you have a ASCP Histo license already, all you have to do is send proof of it to the Florida Board along with whatever the fee is and you can get a Florida Histo license. Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.hannen@parrishmed.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gail Marcella Sent: Wednesday, January 30, 2013 12:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Florida license for Histotechs Hi - I was wondering if anyone knows anything about obtaining a Florida license for Histotechs. I work in NJ , which doesn't require a license, yet, but I also have a NY state License. Can I just transfer it over or do I have to take a test or something? Gail Marcella _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet =================== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" =================== From Timothy.Morken <@t> ucsfmedctr.org Wed Jan 30 13:08:49 2013 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Wed Jan 30 13:09:05 2013 Subject: [Histonet] ATPase without barbital buffer... info Message-ID: <761E2B5697F795489C8710BCC72141FF0535EF@ex07.net.ucsf.edu> Well, I finally found the original papers on the subject of non-barbital buffers for ATPase from the Histonet posting below from Tony Henwood: The reference given for the procedure was : "Loughlin, M. (1993). Muscle biopsy. A laboratory investigation. Butterworth-Heinemann pp.78-79." But that is a book and one not available through our store. So I went online and was able to get the book for ....$2.00. yes, Two Dollars! Now I can give everyone the original references in the book. We have not tried this yet but plan to this year. The book actually has several ATPase methods that do not use barbital buffer on pages 77 - 82 (1993 edition). (One note. The page reference given by Tony's posting below seems to be the Brooke and Kaiser pH 4.3 and 4.6 method on page 81-82 combined with the Round pH 9.4 method on pp 80-81 1) Tris incubating buffer pH 9.4 (Louglin pp 77-79) a. Hayashi and Freiman, An Improved Method of Fixation for Formalin Sensitive Enzymes with Special Reference to Myosin Adenosine Triphosphatase, J Histochem & Cytochem, 1966 14: 577-581 2) Glycine incubating buffer pH 9.4 (Laughlin pp 80-81 a. Round, et. Al. Quick, Simple and Reliable Method for ATPase in Human Muscle Preparations. The Histochemical Journal, Nov 1980, Vol 12, Issue 6, pp 707-710 3) Acetate incubating buffer pH 4.3 and 4.6 ("reverse" ATPase) (Laughlin pp 81-82 a. Brooke, M.H. and Kaiser, K.K., Muscle Fibre Types: How many and what kind? Archives of Neurology, (Chicago), 1970, 23, 369-379 Another non-barbital method is: Khan, M.A., et al, A calcium-citro-phosphate technique for the histochemical localization of myosin ATPase, Stain Technology, 1972, Vol. 47, No. 6, pp. 277-281. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center ************************************************************ ATPase with sodium acetate buffer Tony Henwood AnthonyH <@t> chw.edu.au Sun Aug 20 18:14:40 CDT 2006 Here is our method using acetate buffer in place of barbiturate buffer: Adenosine Triphosphatase (ATPases) [Note: this is the Brooke and Kaiser 4.3 and 4.6 method combined with the Round 9.4 method. tm] Use Demonstration of muscle fibre types. Underlying Principle The principle relies on the ability of the enzyme to remove the terminal phosphate from the ATP, which then combines with calcium in the incubation solution to form an insoluble calcium phosphate. Cobalt is then exchanged for the calcium, which, after reaction with ammonium sulphide, forms a black, insoluble cobalt sulphide at the site of enzyme activity. Fixation and Sectioning Air dried unfixed 8?m cryostat sections Reagents 1. 1% calcium chloride a. Calcium chloride 5.0 g b. Distilled water 500ml 2. 2% Cobalt chloride Warning: Suspected Carcinogen - see MSDS a. Cobalt chloride 10.0 g b. Distilled water 500 ml 3. 1% Ammonium sulphide Warning: Flammable liquid, Irritant, Toxic stench - see MSDS a. 20% ammonium sulphide 0.5 ml b. Distilled water 9.5 ml 4. Acid Pre-incubation medium a. 0.2M Sodium Acetate i. Sodium Acetate Anhydrous 0.82 g ii. Distilled water 50 ml iii. b. 0.2M Acetic Acid i. Glacial Acetic Acid 0.6 ml ii. Distilled water 50 ml 5. 0.2M Acetate Buffer: pH 4.3 pH 4.6 a. 0.2M Sodium Acetate 11 ml 18 ml b. 0.2M Acetic Acid 12 ml 13 ml Adjust to pH 4.3 or 4.6 with 0.2M Sodium Acetate or 0.2M Acetic Acid ? 6. 7.5% Calcium Chloride a. Calcium Chloride 7.5g b. Distilled water 100ml 7. 7.05% Glycine a. Glycine 7.05g b. Distilled water 100ml Freeze in 2ml aliquots, defrost one aliquot before use. 8. 5.625% Sodium Chloride a. Sodium Chloride 5.625g b. Distilled water 100ml 9. 3.5% Sodim Hydroxide a. Sodium Hydroxide 3.5g b. Distilled water 100ml 10. Alkaline Stock Warning: Irritant - see MSDS a. 7.05% Glycine 2ml b. 7.5% Calcium Chloride 2ml c. 5.625% Sodium Chloride 2ml d. 3.5% Sodium Hydroxide 2ml e. Distilled water 42ml 11. Incubating Medium a. Alkaline Stock 25 ml b. ATP (Sigma A-7699) 40 mg Adjust to pH 9.4 - 9.5 with 0.1M HCl Method for preparing 9.4 Substrate Solution 1. Prepare fresh Alkaline Stock for each run 2. Place in 37oC Oven for 20minutes 3. pH to 11 using 0.1M NaOH (to activate the ATP) 4. Add ATP 5. pH to 9.4 using 0.1M HCl Staining Method 1. Place one slide in 4.3 and one in 4.6 buffer at room temperature for 20 minutes 2. Wash each in distilled water 3 times 3. Place these slides (one from the 4.3 solution , one from 4.6 solution and the 9.4 slide) in the 9.4 substrate at 37?C for: a. pH 9.4 10 mins b. pH 4.6 30 mins c. pH 4.3 45 mins 4. Place slides in 2 changes of 1% calcium chloride 3 min each 5. Place slides in 2% cobalt chloride 3 min 6. Wash well in distilled water 7. In fume cupboard drain slides well and place in ammonium sulphide solution for 1 min 8. Wash well in tap water 9. Dehydrate clear and mount. Results pH 9.4 Type 1 fibres pale Type 2A fibres intermediate Type 2B fibres dark pH 4.3 and pH 4.6 pH 4.3 pH 4.6 Type 1 fibres dark dark Type 2A fibres pale pale Type 2B fibres pale intermediate Type 2C fibres intermediate dark Notes This is a complicated stain and there are several areas in which one needs to be careful in order to achieve a good fibre type differentiation. 1. The pH of all solutions is critical 2. Timing is crucial a. The stock ammonium sulfide must still be yellow. As it ages or oxidizes, it becomes more red and cannot be used. 3. The pH of all solutions must be adjusted at the temperature they will be used. References 1. Loughlin, M. (1993). Muscle biopsy. A laboratory investigation. Butterworth-Heinemann pp.78-79. [actually pp 81-82. tm] Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 From Ronald.Houston <@t> nationwidechildrens.org Wed Jan 30 13:10:39 2013 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Wed Jan 30 13:10:50 2013 Subject: [Histonet] Kaposi's Sarcoma Message-ID: Does anyone have a paraffin block of Kaposi's they could share? Thanks Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org "One person with passion is better than forty people merely interested." ~ E.M. Forster From ibernard <@t> uab.edu Wed Jan 30 13:25:38 2013 From: ibernard <@t> uab.edu (Ian R Bernard) Date: Wed Jan 30 13:25:43 2013 Subject: [Histonet] RE: cytoprep In-Reply-To: <906B4DA90ED1DB4DB6C7E94D7CEE6C36220C8FB4@PEITHA.wad.pa-ucl.com> References: <906B4DA90ED1DB4DB6C7E94D7CEE6C36220C8FB4@PEITHA.wad.pa-ucl.com> Message-ID: Our rehydration time is 30-45 secs. Then into 95% alcohol. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt Sent: Wednesday, January 30, 2013 12:18 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cytoprep Hi to all- I am continuing to have a problem with Pap stains done by hand: 1. We rehydrate 25 seconds in saline and straight into alcohol. Thicker areas are ok, but thinner areas appear to be air dried...... 2. Red cell ghosts are NOT clearing I believe there are quite a few Histology labs also performing cytoprep work and I will appreciate any thoughts you may have. Nancy Schmitt HT, MLT Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From patpxs <@t> gmail.com Wed Jan 30 14:43:00 2013 From: patpxs <@t> gmail.com (Paula Sicurello) Date: Wed Jan 30 14:43:08 2013 Subject: [Histonet] ATPase without barbital buffer... info In-Reply-To: <761E2B5697F795489C8710BCC72141FF0535EF@ex07.net.ucsf.edu> References: <761E2B5697F795489C8710BCC72141FF0535EF@ex07.net.ucsf.edu> Message-ID: Hi Tim, Thanks for finding those protocols for us! Perhaps we can all move away from using a controlled substance (what a nightmare to purchase). Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 On Wed, Jan 30, 2013 at 2:08 PM, Morken, Timothy wrote: > Well, I finally found the original papers on the subject of non-barbital buffers for ATPase from the Histonet posting below from Tony Henwood: > > The reference given for the procedure was : "Loughlin, M. (1993). Muscle biopsy. A laboratory investigation. Butterworth-Heinemann pp.78-79." > > But that is a book and one not available through our store. So I went online and was able to get the book for ....$2.00. yes, Two Dollars! > > Now I can give everyone the original references in the book. We have not tried this yet but plan to this year. > > The book actually has several ATPase methods that do not use barbital buffer on pages 77 - 82 (1993 edition). > > (One note. The page reference given by Tony's posting below seems to be the Brooke and Kaiser pH 4.3 and 4.6 method on page 81-82 combined with the Round pH 9.4 method on pp 80-81 > > > 1) Tris incubating buffer pH 9.4 (Louglin pp 77-79) > > a. Hayashi and Freiman, An Improved Method of Fixation for Formalin Sensitive Enzymes with Special Reference to Myosin Adenosine Triphosphatase, J Histochem & Cytochem, 1966 14: 577-581 > > 2) Glycine incubating buffer pH 9.4 (Laughlin pp 80-81 > > a. Round, et. Al. Quick, Simple and Reliable Method for ATPase in Human Muscle Preparations. The Histochemical Journal, Nov 1980, Vol 12, Issue 6, pp 707-710 > > 3) Acetate incubating buffer pH 4.3 and 4.6 ("reverse" ATPase) (Laughlin pp 81-82 > > a. Brooke, M.H. and Kaiser, K.K., Muscle Fibre Types: How many and what kind? Archives of Neurology, (Chicago), 1970, 23, 369-379 > Another non-barbital method is: > Khan, M.A., et al, A calcium-citro-phosphate technique for the histochemical localization of myosin ATPase, Stain Technology, 1972, Vol. 47, No. 6, pp. 277-281. > > Tim Morken > Supervisor, Electron Microscopy/Neuromuscular Special Studies > Department of Pathology > UC San Francisco Medical Center > ************************************************************ > > ATPase with sodium acetate buffer > > Tony Henwood AnthonyH <@t> chw.edu.au > Sun Aug 20 18:14:40 CDT 2006 > Here is our method using acetate buffer in place of barbiturate buffer: > > Adenosine Triphosphatase (ATPases) [Note: this is the Brooke and Kaiser 4.3 and 4.6 method > combined with the Round 9.4 method. tm] > > Use > > Demonstration of muscle fibre types. > > Underlying Principle > > The principle relies on the ability of the enzyme to remove the terminal phosphate from the ATP, > which then combines with calcium in the incubation solution to form an insoluble calcium > phosphate. Cobalt is then exchanged for the calcium, which, after reaction with ammonium > sulphide, forms a black, insoluble cobalt sulphide at the site of enzyme activity. > > Fixation and Sectioning > > Air dried unfixed 8?m cryostat sections > > Reagents > > 1. 1% calcium chloride > a. Calcium chloride 5.0 g > b. Distilled water 500ml > > 2. 2% Cobalt chloride > Warning: Suspected Carcinogen - see MSDS > a. Cobalt chloride 10.0 g > b. Distilled water 500 ml > > 3. 1% Ammonium sulphide > Warning: Flammable liquid, Irritant, Toxic stench - see MSDS > a. 20% ammonium sulphide 0.5 ml > b. Distilled water 9.5 ml > > 4. Acid Pre-incubation medium > a. 0.2M Sodium Acetate > i. Sodium Acetate Anhydrous 0.82 g > ii. Distilled water 50 ml > iii. > b. 0.2M Acetic Acid > i. Glacial Acetic Acid 0.6 ml > ii. Distilled water 50 ml > > > 5. 0.2M Acetate Buffer: pH 4.3 pH 4.6 > a. 0.2M Sodium Acetate 11 ml 18 ml > b. 0.2M Acetic Acid 12 ml 13 ml > > Adjust to pH 4.3 or 4.6 with 0.2M Sodium Acetate or 0.2M Acetic Acid > ? > > 6. 7.5% Calcium Chloride > a. Calcium Chloride 7.5g > b. Distilled water 100ml > > 7. 7.05% Glycine > a. Glycine 7.05g > b. Distilled water 100ml > > Freeze in 2ml aliquots, defrost one aliquot before use. > > 8. 5.625% Sodium Chloride > a. Sodium Chloride 5.625g > b. Distilled water 100ml > > 9. 3.5% Sodim Hydroxide > a. Sodium Hydroxide 3.5g > b. Distilled water 100ml > > 10. Alkaline Stock > Warning: Irritant - see MSDS > a. 7.05% Glycine 2ml > b. 7.5% Calcium Chloride 2ml > c. 5.625% Sodium Chloride 2ml > d. 3.5% Sodium Hydroxide 2ml > e. Distilled water 42ml > > 11. Incubating Medium > a. Alkaline Stock 25 ml > b. ATP (Sigma A-7699) 40 mg > > Adjust to pH 9.4 - 9.5 with 0.1M HCl > > Method for preparing 9.4 Substrate Solution > > 1. Prepare fresh Alkaline Stock for each run > 2. Place in 37oC Oven for 20minutes > 3. pH to 11 using 0.1M NaOH (to activate the ATP) > 4. Add ATP > 5. pH to 9.4 using 0.1M HCl > > Staining Method > > 1. Place one slide in 4.3 and one in 4.6 buffer at room temperature for 20 minutes > 2. Wash each in distilled water 3 times > 3. Place these slides (one from the 4.3 solution , one from 4.6 solution and the 9.4 slide) in the 9.4 > substrate at 37?C for: > a. pH 9.4 10 mins > b. pH 4.6 30 mins > c. pH 4.3 45 mins > 4. Place slides in 2 changes of 1% calcium chloride 3 min each > 5. Place slides in 2% cobalt chloride 3 min > 6. Wash well in distilled water > 7. In fume cupboard drain slides well and place in ammonium sulphide solution for 1 min > 8. Wash well in tap water > 9. Dehydrate clear and mount. > > > > > Results > > pH 9.4 > Type 1 fibres pale > Type 2A fibres intermediate > Type 2B fibres dark > > > pH 4.3 and pH 4.6 > pH 4.3 pH 4.6 > Type 1 fibres dark dark > Type 2A fibres pale pale > Type 2B fibres pale intermediate > Type 2C fibres intermediate dark > > > Notes > > This is a complicated stain and there are several areas in which one needs to be careful in order to > achieve a good fibre type differentiation. > > 1. The pH of all solutions is critical > 2. Timing is crucial > a. The stock ammonium sulfide must still be yellow. As it ages or oxidizes, it becomes > more red and cannot be used. > 3. The pH of all solutions must be adjusted at the temperature they will be used. > > > > References > > 1. Loughlin, M. (1993). Muscle biopsy. A laboratory investigation. Butterworth-Heinemann > pp.78-79. [actually pp 81-82. tm] > > Regards > > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) > Laboratory Manager & Senior Scientist > The Children's Hospital at Westmead, > Locked Bag 4001, Westmead, 2145, AUSTRALIA. > Tel: 612 9845 3306 > Fax: 612 9845 3318 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mohit.koladia <@t> ndsu.edu Wed Jan 30 15:38:38 2013 From: mohit.koladia <@t> ndsu.edu (Mohit Koladia) Date: Wed Jan 30 15:38:42 2013 Subject: [Histonet] Query for Thionin/ Acid fucshin staining Message-ID: Hello histonetter, I was looking for the best protocol for thionin/acid fucshin staining. I was looking for details if someone has experience with same. I have paraffin embedded 5 micron sections of rat brains. Also, i want to know if thionin or cresyl violet makes some difference to the staining pattern and the purpose of using each. Thanks, Mohit Koladia *Grad Student,* *NDSU, Fargo* From sdysart <@t> mirnarx.com Wed Jan 30 15:53:59 2013 From: sdysart <@t> mirnarx.com (Sarah Dysart) Date: Wed Jan 30 15:54:12 2013 Subject: [Histonet] Cytology Message-ID: <8A70A9B2ECDD084DACFE6C59FCF86D50484FA9EA@BL2PRD0711MB434.namprd07.prod.outlook.com> So....do you guys think it would be ok to leave cytospins in Penfix overnight in the fridge (4C), or would it degrade the cells? I'm doing Penfix instead of 95% because I need the formalin linkage on the cells... Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 From jheare <@t> lsu.edu Wed Jan 30 16:00:43 2013 From: jheare <@t> lsu.edu (Jake E Heare) Date: Wed Jan 30 16:03:53 2013 Subject: [Histonet] Update: Tissue Processing and Embedding Lab near Baton Rouge Message-ID: <4D02AF1E5747E543BD00548C6B42276653973244@BY2PRD0610MB352.namprd06.prod.outlook.com> Thanks everyone who has replied to my initial post. I'm so happy to know that there are many helpful people out there. I have been in contact with a histology consultant in Baton Rouge who regularly does oyster tissue processing. I'm super excited to finish my project in the next 6 months. Again thank you everyone for helping me out. Have a great evening! Jake From tony.henwood <@t> health.nsw.gov.au Wed Jan 30 16:04:39 2013 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Wed Jan 30 16:04:53 2013 Subject: [Histonet] RE: cytoprep In-Reply-To: <906B4DA90ED1DB4DB6C7E94D7CEE6C36220C8FB4@PEITHA.wad.pa-ucl.com> References: <906B4DA90ED1DB4DB6C7E94D7CEE6C36220C8FB4@PEITHA.wad.pa-ucl.com> Message-ID: <6D6BD1DE8A5571489398B392A38A71579D22E99E@xmdb04.nch.kids> The saline re-hydration works well as long as smears are not allowed to dry too long before rehydration. I have found that air-drying longer than 2 hours decreases the effectiveness of the procedure. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt Sent: Thursday, 31 January 2013 5:18 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cytoprep Hi to all- I am continuing to have a problem with Pap stains done by hand: 1. We rehydrate 25 seconds in saline and straight into alcohol. Thicker areas are ok, but thinner areas appear to be air dried...... 2. Red cell ghosts are NOT clearing I believe there are quite a few Histology labs also performing cytoprep work and I will appreciate any thoughts you may have. Nancy Schmitt HT, MLT Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From tony.henwood <@t> health.nsw.gov.au Wed Jan 30 16:07:31 2013 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Wed Jan 30 16:07:46 2013 Subject: [Histonet] RE: ATPase without barbital buffer... info In-Reply-To: <761E2B5697F795489C8710BCC72141FF0535EF@ex07.net.ucsf.edu> References: <761E2B5697F795489C8710BCC72141FF0535EF@ex07.net.ucsf.edu> Message-ID: <6D6BD1DE8A5571489398B392A38A71579D22E9C4@xmdb04.nch.kids> Hi Tim, Well done Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Thursday, 31 January 2013 6:09 AM To: Histonet Subject: [Histonet] ATPase without barbital buffer... info Well, I finally found the original papers on the subject of non-barbital buffers for ATPase from the Histonet posting below from Tony Henwood: The reference given for the procedure was : "Loughlin, M. (1993). Muscle biopsy. A laboratory investigation. Butterworth-Heinemann pp.78-79." But that is a book and one not available through our store. So I went online and was able to get the book for ....$2.00. yes, Two Dollars! Now I can give everyone the original references in the book. We have not tried this yet but plan to this year. The book actually has several ATPase methods that do not use barbital buffer on pages 77 - 82 (1993 edition). (One note. The page reference given by Tony's posting below seems to be the Brooke and Kaiser pH 4.3 and 4.6 method on page 81-82 combined with the Round pH 9.4 method on pp 80-81 1) Tris incubating buffer pH 9.4 (Louglin pp 77-79) a. Hayashi and Freiman, An Improved Method of Fixation for Formalin Sensitive Enzymes with Special Reference to Myosin Adenosine Triphosphatase, J Histochem & Cytochem, 1966 14: 577-581 2) Glycine incubating buffer pH 9.4 (Laughlin pp 80-81 a. Round, et. Al. Quick, Simple and Reliable Method for ATPase in Human Muscle Preparations. The Histochemical Journal, Nov 1980, Vol 12, Issue 6, pp 707-710 3) Acetate incubating buffer pH 4.3 and 4.6 ("reverse" ATPase) (Laughlin pp 81-82 a. Brooke, M.H. and Kaiser, K.K., Muscle Fibre Types: How many and what kind? Archives of Neurology, (Chicago), 1970, 23, 369-379 Another non-barbital method is: Khan, M.A., et al, A calcium-citro-phosphate technique for the histochemical localization of myosin ATPase, Stain Technology, 1972, Vol. 47, No. 6, pp. 277-281. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center ************************************************************ ATPase with sodium acetate buffer Tony Henwood AnthonyH <@t> chw.edu.au Sun Aug 20 18:14:40 CDT 2006 Here is our method using acetate buffer in place of barbiturate buffer: Adenosine Triphosphatase (ATPases) [Note: this is the Brooke and Kaiser 4.3 and 4.6 method combined with the Round 9.4 method. tm] Use Demonstration of muscle fibre types. Underlying Principle The principle relies on the ability of the enzyme to remove the terminal phosphate from the ATP, which then combines with calcium in the incubation solution to form an insoluble calcium phosphate. Cobalt is then exchanged for the calcium, which, after reaction with ammonium sulphide, forms a black, insoluble cobalt sulphide at the site of enzyme activity. Fixation and Sectioning Air dried unfixed 8?m cryostat sections Reagents 1. 1% calcium chloride a. Calcium chloride 5.0 g b. Distilled water 500ml 2. 2% Cobalt chloride Warning: Suspected Carcinogen - see MSDS a. Cobalt chloride 10.0 g b. Distilled water 500 ml 3. 1% Ammonium sulphide Warning: Flammable liquid, Irritant, Toxic stench - see MSDS a. 20% ammonium sulphide 0.5 ml b. Distilled water 9.5 ml 4. Acid Pre-incubation medium a. 0.2M Sodium Acetate i. Sodium Acetate Anhydrous 0.82 g ii. Distilled water 50 ml iii. b. 0.2M Acetic Acid i. Glacial Acetic Acid 0.6 ml ii. Distilled water 50 ml 5. 0.2M Acetate Buffer: pH 4.3 pH 4.6 a. 0.2M Sodium Acetate 11 ml 18 ml b. 0.2M Acetic Acid 12 ml 13 ml Adjust to pH 4.3 or 4.6 with 0.2M Sodium Acetate or 0.2M Acetic Acid ? 6. 7.5% Calcium Chloride a. Calcium Chloride 7.5g b. Distilled water 100ml 7. 7.05% Glycine a. Glycine 7.05g b. Distilled water 100ml Freeze in 2ml aliquots, defrost one aliquot before use. 8. 5.625% Sodium Chloride a. Sodium Chloride 5.625g b. Distilled water 100ml 9. 3.5% Sodim Hydroxide a. Sodium Hydroxide 3.5g b. Distilled water 100ml 10. Alkaline Stock Warning: Irritant - see MSDS a. 7.05% Glycine 2ml b. 7.5% Calcium Chloride 2ml c. 5.625% Sodium Chloride 2ml d. 3.5% Sodium Hydroxide 2ml e. Distilled water 42ml 11. Incubating Medium a. Alkaline Stock 25 ml b. ATP (Sigma A-7699) 40 mg Adjust to pH 9.4 - 9.5 with 0.1M HCl Method for preparing 9.4 Substrate Solution 1. Prepare fresh Alkaline Stock for each run 2. Place in 37oC Oven for 20minutes 3. pH to 11 using 0.1M NaOH (to activate the ATP) 4. Add ATP 5. pH to 9.4 using 0.1M HCl Staining Method 1. Place one slide in 4.3 and one in 4.6 buffer at room temperature for 20 minutes 2. Wash each in distilled water 3 times 3. Place these slides (one from the 4.3 solution , one from 4.6 solution and the 9.4 slide) in the 9.4 substrate at 37?C for: a. pH 9.4 10 mins b. pH 4.6 30 mins c. pH 4.3 45 mins 4. Place slides in 2 changes of 1% calcium chloride 3 min each 5. Place slides in 2% cobalt chloride 3 min 6. Wash well in distilled water 7. In fume cupboard drain slides well and place in ammonium sulphide solution for 1 min 8. Wash well in tap water 9. Dehydrate clear and mount. Results pH 9.4 Type 1 fibres pale Type 2A fibres intermediate Type 2B fibres dark pH 4.3 and pH 4.6 pH 4.3 pH 4.6 Type 1 fibres dark dark Type 2A fibres pale pale Type 2B fibres pale intermediate Type 2C fibres intermediate dark Notes This is a complicated stain and there are several areas in which one needs to be careful in order to achieve a good fibre type differentiation. 1. The pH of all solutions is critical 2. Timing is crucial a. The stock ammonium sulfide must still be yellow. As it ages or oxidizes, it becomes more red and cannot be used. 3. The pH of all solutions must be adjusted at the temperature they will be used. References 1. Loughlin, M. (1993). Muscle biopsy. A laboratory investigation. Butterworth-Heinemann pp.78-79. [actually pp 81-82. tm] Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From joewalker <@t> rrmc.org Thu Jan 31 09:15:53 2013 From: joewalker <@t> rrmc.org (Joe W. Walker, Jr.) Date: Thu Jan 31 09:16:19 2013 Subject: [Histonet] RE: cytoprep In-Reply-To: <6D6BD1DE8A5571489398B392A38A71579D22E99E@xmdb04.nch.kids> References: <906B4DA90ED1DB4DB6C7E94D7CEE6C36220C8FB4@PEITHA.wad.pa-ucl.com> <6D6BD1DE8A5571489398B392A38A71579D22E99E@xmdb04.nch.kids> Message-ID: <3C2378778400AD448ADA6FD6BDB7CCCC179DA5A1@RRMBX03.rrmc.local> I assume that these are conventional Pap tests where the material is smeared onto the slide. I am curious why the slides are air dried upon collection instead of applying a spray fixative to the slide. If these are non-gyn slides from a brushing or FNA, then we might need to have a different discussion. As someone with numerous years experience with cytoprep, I would need more information to help trouble shoot what might be the real issue and to offer suggestions. Cheers, Joe W. Walker, Jr. MS, SCT(ASCP)CM Anatomical Pathology Manager Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P: 802.747.1790 F: 802.747.6525 NEW EMAIL: joewalker@rrmc.org www.rrmc.org Our Vision: To be the Best Community Healthcare System in New England Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet Recognition? and the Governor's Award for Performance Excellence -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony Henwood (SCHN) Sent: Wednesday, January 30, 2013 5:05 PM To: 'Nancy Schmitt'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: cytoprep The saline re-hydration works well as long as smears are not allowed to dry too long before rehydration. I have found that air-drying longer than 2 hours decreases the effectiveness of the procedure. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt Sent: Thursday, 31 January 2013 5:18 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cytoprep Hi to all- I am continuing to have a problem with Pap stains done by hand: 1. We rehydrate 25 seconds in saline and straight into alcohol. Thicker areas are ok, but thinner areas appear to be air dried...... 2. Red cell ghosts are NOT clearing I believe there are quite a few Histology labs also performing cytoprep work and I will appreciate any thoughts you may have. Nancy Schmitt HT, MLT Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. Thank You From enrriq88 <@t> yahoo.com Thu Jan 31 09:35:54 2013 From: enrriq88 <@t> yahoo.com (enrriq88@yahoo.com) Date: Thu Jan 31 09:35:58 2013 Subject: [Histonet] hey Message-ID: <1359646554.15482.androidMobile@web120806.mail.ne1.yahoo.com> hey have a look http://bit.ly/14tqqeH From jerry.santiago <@t> bellsouth.net Thu Jan 31 10:04:34 2013 From: jerry.santiago <@t> bellsouth.net (=?utf-8?B?amVycnkuc2FudGlhZ29AYmVsbHNvdXRoLm5ldA==?=) Date: Thu Jan 31 10:04:45 2013 Subject: =?utf-8?B?RndkOiBSZTogW0hpc3RvbmV0XSBGbG9yaWRhIGxpY2Vuc2UgZm9yIEhpc3RvdGVj?= =?utf-8?B?aHM=?= Message-ID: <280332.87649.bm@smtp111.mail.ne1.yahoo.com> Sent from my HTC smartphone on the Now Network from Sprint! ----- Forwarded message ----- From: "jerry.santiago@bellsouth.net" To: "Gail Marcella" Subject: Re: [Histonet] Florida license for Histotechs Date: Thu, Jan 31, 2013 10:58 am Hi Gail, In order to get a license in Florida you will to be ASCP certified, provide 2 hours in medical errors and 1 hour in HIV/AIDS. There are 3 levels of licensure. Technician, technologist and supervisors. You can get the qualifications at the Medical Quality Assurance website. Florida does not recognize licensure from any other state. Jerry Santiago, MSEd., HTL(ASCP)QIHC HT Program Director Florida State College at Jacksonville T: 904/766-6528 Email: jerry.Santiago@fscj.edu Sent from my HTC smartphone on the Now Network from Sprint!----- Reply message ----- From: "Gail Marcella" To: "histonet@lists.utsouthwestern.edu" Subject: [Histonet] Florida license for Histotechs Date: Wed, Jan 30, 2013 12:34 pm Hi - I was wondering if anyone knows anything about obtaining a Florida license for Histotechs. I work in NJ , which doesn't require a license, yet, but I also have a NY state License. Can I just transfer it over or do I have to take a test or something? Gail Marcella _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From enrriq88 <@t> yahoo.com Thu Jan 31 10:50:24 2013 From: enrriq88 <@t> yahoo.com (enrriq88@yahoo.com) Date: Thu Jan 31 10:50:28 2013 Subject: [Histonet] hey Message-ID: <1359651024.22573.androidMobile@web120806.mail.ne1.yahoo.com> check this page out http://bit.ly/W1EKau From christina.thurby <@t> bms.com Thu Jan 31 11:10:04 2013 From: christina.thurby <@t> bms.com (Thurby, Christina) Date: Thu Jan 31 11:13:24 2013 Subject: [Histonet] Question about sectioning brain tissue at 10-15 microns Message-ID: Hi, Can anyone with experience in cutting thick FFPE brain sections please give advice on how to get a thick brain section without it curling up before it gets to the waterbath? We are doing okay at cutting these at 10 microns, but are trying to get some 12 micron sections and the section just curls as it is being cut on the microtome. We use Paraplast - just in case anyone needs to know. I did read that having a lower temp melting point paraffin sometimes helps with this issue. Any other thoughts from folks that routinely do this? Thanks! Christina Thurby Bristol Myers Squibb 812-307-2093 ________________________________ This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. From TNMayer <@t> mdanderson.org Thu Jan 31 11:21:27 2013 From: TNMayer <@t> mdanderson.org (Mayer,Toysha N) Date: Thu Jan 31 11:21:32 2013 Subject: [Histonet] . Re: Florida license for Histotechs (Brendal Finlay) Message-ID: <47E9B2C01DDDD94881EACD2DC44EBC88042660@D1PWPEXMBX05.mdanderson.edu> Along that line, what are the requirements for a NY license? I have an HTL student who wants to move there after graduation. One interpretation was to go to school in the state, another was to do a clinical rotation in the state, and still a third was to submit the HT scores and pay the fee (from NY state society). The last one doesn't sound right, because what if you are an HTL, would you still have to take the HT exam? I did advise the student to call the state board, an HT program, or a hospital in NY to get a clearer understanding. Toysha N. Mayer, MBA, HT (ASCP) Instructor, Education Coordinator Program in Histotechnology School of Health Professions MD Anderson Cancer Center (713) 563-3481 tnmayer@mdanderson.org Message: 1 Date: Wed, 30 Jan 2013 11:59:44 -0600 From: Brendal Finlay Subject: Re: [Histonet] Florida license for Histotechs To: Gail Marcella Cc: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=us-ascii Here is the link for qualification information through the Florida Department of Medical Quality Assurance. http://www.doh.state.fl.us/mqa/ClinLab/clp_lic_req.html On Jan 30, 2013, at 11:34 AM, Gail Marcella wrote: > Hi - I was wondering if anyone knows anything about obtaining a Florida license for Histotechs. I work in NJ , which doesn't require a license, yet, but I also have a NY state License. Can I just transfer it over or do I have to take a test or something? > Gail Marcella > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ************* From Nancy_Schmitt <@t> pa-ucl.com Thu Jan 31 11:23:35 2013 From: Nancy_Schmitt <@t> pa-ucl.com (Nancy Schmitt) Date: Thu Jan 31 11:23:39 2013 Subject: [Histonet] cytoprep Message-ID: <906B4DA90ED1DB4DB6C7E94D7CEE6C36220C916F@PEITHA.wad.pa-ucl.com> Sorry I left out a few details. These paps are for non-gyn smears mostly from FNA collection. I am continuing to have a problem with Pap stains done by hand: 1. We rehydrate 25 seconds in saline and straight into alcohol. Thicker areas are ok, but thinner areas appear to be air dried...... 2. Red cell ghosts are NOT clearing I believe there are quite a few Histology labs also performing cytoprep work and I will appreciate any thoughts you may have. Nancy Schmitt HT, MLT Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From TJohnson <@t> gnf.org Thu Jan 31 11:26:43 2013 From: TJohnson <@t> gnf.org (Teri Johnson) Date: Thu Jan 31 11:26:49 2013 Subject: [Histonet] Re: Question about sectioning brain tissue at 10-15 microns Message-ID: <9F3CFEE76E51B64991C7485270890B402E712DCC@EX4.lj.gnf.org> Hi Christina, First I would have you use a room temperature block. If the tissue needs to be rehydrated briefly, use room temp water instead of ice. I can get 12 micron ribbons of bone using Paraplast extra, I haven't tried thicker. If that doesn't work, what blade are you using? In my experience, the Accuedge low profile blades tend to curl more quickly than other blades. You can try using a thicker blade or one from a different manufacturer and see if that helps. Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 From twheelock <@t> mclean.harvard.edu Thu Jan 31 11:31:25 2013 From: twheelock <@t> mclean.harvard.edu (Tim Wheelock) Date: Thu Jan 31 11:31:31 2013 Subject: [Histonet] Question about sectioning brain tissue at 10-15 microns Message-ID: <510AAA6D.3020302@mclean.harvard.edu> Hi Christina: I use Paraplast to infiltrate the formalin fixed brain tissue, but I use Surgipath Embedding Medium from Leica (Catalogue number 3801320) to embed the tissue. (Surgipath does have about the same melting point as the Paraplast) Even the 10 micron sections (for the Congo red stain) tend to curl, but I just guide them off of the disposable blade with a brush or a pick. Hope this helps, Tim Tim Wheelock Harvard Brain Bank McLean Hospital Belmont MA 617-855-3592 From jpiche-grocki <@t> wtbyhosp.org Thu Jan 31 11:36:34 2013 From: jpiche-grocki <@t> wtbyhosp.org (PicheGrocki, Jessica) Date: Thu Jan 31 11:36:40 2013 Subject: [Histonet] No expiration dates on chemicals Message-ID: <631955447A364B45B9458D2905635110655BE248@WIN08-MBX-01.wtbyhosp.org> Hi All, Just wondering what everyone is doing in regards the ANP question involving expiration dates on chemicals and reagents? Is everyone getting rid of old chemicals that have been opened for 10 years or more? Please advise. Have a good day and thank you, Jessica Piche', HT (ASCP) Waterbury Hospital Histology Lab CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital From jtyrrell <@t> med.wayne.edu Thu Jan 31 13:22:44 2013 From: jtyrrell <@t> med.wayne.edu (Tyrrell, Jannifer) Date: Thu Jan 31 13:22:57 2013 Subject: [Histonet] RE: Histonet Question Message-ID: <68201B9EAFED334FAF2CA1F1A18CBF790C9229D6@MED-CORE05B.med.wayne.edu> I'm new to the Histonet website, but not new to histotechnology. I'm trying to salvage some irreplaceable Zucker Diabetic Fatty rat tissue samples that were embedded in paraffin in May 2009. They have sat on my bench since they were embedded as we thought the entire experiment was a huge failure. However, recent examination of some of the flash-frozen tissues has shown us that the experiment was actually a huge success. Which leads me to my issues and question... I needed to section these tissues (liver & pancreas) and have attempted to do so by several troubleshooting means - soaking the block in warm water then rapid cooling, place ice on the block then rapid slicing, changing the blade, using a different microtome, changing the blade angle, you name it, I think I've tried it. Now I've got a bunch of sections that look like accordions that will not flatten, but hardly anything left in the actual blocks. These horrible sections are on slides, but not stained yet. I only put them on slides because I NEED the tissues. But to stain the sections as they are would be a waste of the tissues and the reagents. I saw a protocol for formol-glycerol re-hydration and have thought about using it...but now I can't get the tissue sections off of the glass slides. ANY and all help or advice would be INCREDIBLY and GREATLY appreciated! This is the last bit of work I have to complete in my dissertation project and the data, I'm certain, will be astounding. Jannifer Tyrrell NIEHS/NIH-Sponsored Pre-Doctoral Ruth Kirschstein Federal Grant Recipient PhD Candidate Department of Pathology Wayne State University School of Medicine 550 E. Canfield Ave. 111 Lande Building Detroit, MI 48201 jtyrrell@med.wayne.edu From patpxs <@t> gmail.com Thu Jan 31 14:49:44 2013 From: patpxs <@t> gmail.com (Paula Sicurello) Date: Thu Jan 31 14:49:47 2013 Subject: [Histonet] No expiration dates on chemicals In-Reply-To: <631955447A364B45B9458D2905635110655BE248@WIN08-MBX-01.wtbyhosp.org> References: <631955447A364B45B9458D2905635110655BE248@WIN08-MBX-01.wtbyhosp.org> Message-ID: We keep ours no longer than 5 years. If there is no expiration date on the bottle when we receive it, we mark it as expired 5 years from date of receipt. Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 On Thu, Jan 31, 2013 at 12:36 PM, PicheGrocki, Jessica wrote: > Hi All, > > Just wondering what everyone is doing in regards the ANP question involving expiration dates on chemicals and reagents? Is everyone getting rid of old chemicals that have been opened for 10 years or more? > > Please advise. > > Have a good day and thank you, > > Jessica Piche', HT (ASCP) > Waterbury Hospital Histology Lab > > > > CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kim-lake <@t> uiowa.edu Thu Jan 31 15:04:37 2013 From: kim-lake <@t> uiowa.edu (Lake, Kim S) Date: Thu Jan 31 15:04:51 2013 Subject: [Histonet] Histotech opening -- Iowa City, IA Message-ID: There is an opening for a full-time 1st shift histotech at the Oral Pathology Laboratory at the University of Iowa College of Dentistry. Full details can be found at https://jobs.uiowa.edu/pands/view/62184 Education Required: A Bachelor's degree in a basic science with specialized training in medical technology or a clinical laboratory specialty or an equivalent combination of education and experience is required. Experience Required: *1-3 years clinical laboratory experience is required. *Excellent written and verbal communication skills are required. Desirable Qualifications: *Certification by the American Society of Clinical Pathologists. *Proven ability to work in high volume work enviroment. *Some (6 months to 1 year) experience with Microsoft Office Suite. Kim Lake Laboratory Manager Oral Pathology Laboratory University of Iowa College of Dentistry Phone (319) 384 4433 Fax (319) 353 5569 From liz <@t> premierlab.com Thu Jan 31 15:06:16 2013 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Thu Jan 31 15:08:41 2013 Subject: [Histonet] No expiration dates on chemicals In-Reply-To: References: <631955447A364B45B9458D2905635110655BE248@WIN08-MBX-01.wtbyhosp.org>, Message-ID: <14E2C6176416974295479C64A11CB9AE016411AB45D0@SBS2K8.premierlab.local> We also set an expiration date of 5 years on dry chemicals with no expiration date on delivery. After the 5 years they are set aside to be retested, once retested they are placed back in use. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 881-0763 cell (303) 682-9060 fax liz@premierlab.com Ship to address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello [patpxs@gmail.com] Sent: Thursday, January 31, 2013 1:49 PM To: PicheGrocki, Jessica Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] No expiration dates on chemicals We keep ours no longer than 5 years. If there is no expiration date on the bottle when we receive it, we mark it as expired 5 years from date of receipt. Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 On Thu, Jan 31, 2013 at 12:36 PM, PicheGrocki, Jessica wrote: > Hi All, > > Just wondering what everyone is doing in regards the ANP question involving expiration dates on chemicals and reagents? Is everyone getting rid of old chemicals that have been opened for 10 years or more? > > Please advise. > > Have a good day and thank you, > > Jessica Piche', HT (ASCP) > Waterbury Hospital Histology Lab > > > > CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ltougas <@t> dawsoncollege.qc.ca Thu Jan 31 15:22:21 2013 From: ltougas <@t> dawsoncollege.qc.ca (Liette Tougas) Date: Thu Jan 31 15:22:26 2013 Subject: [Histonet] standards for microtomy bench Message-ID: <455897B94CF3A44F81F727160F23FB110C7E996E@DC229.ad.dawsoncollege.qc.ca> Hi everyone, I would like to know if anyone has standards (from any country!) regarding the ideal amount of space for a microtomy workbench eg: * If facing a wall: dept usually required. * Width of individual counter space which would include the microtome, floatation bath, rack for cut sections. (blocks kept cold in fridgitray nearby) * Suggested dept underneath working counter (towards the back for leg room) Any other suggestion or elements to take into account when designing microtomy benches? Thank you very much in advance for any precious advice! Liette Tougas, RT, B.Sc., M.Sc. , faculty Biomedical Laboratory Technology Department Dawson College, Montreal, Qc From wdesalvo.cac <@t> outlook.com Thu Jan 31 15:48:50 2013 From: wdesalvo.cac <@t> outlook.com (WILLIAM DESALVO) Date: Thu Jan 31 15:48:56 2013 Subject: [Histonet] No expiration dates on chemicals In-Reply-To: References: <631955447A364B45B9458D2905635110655BE248@WIN08-MBX-01.wtbyhosp.org>, Message-ID: Dry chemicals can have an extremely long shelf life. I once visited Chuck Churukian's lab (god rest his sole) and he had a wall of dry chemicals, and quite proud, that many were well over 20 years old. Test and retest before you discard. Liquid reagents can often have an extended shelf life and often past the manufacturers expiration date. For patient safety and quality consistency, I suggest you should test for efficacy of the chemical or reagent before discarding. If it continues to perform as indicated, then you can continue to use. Remember to label the chemical from the date of testing and set a new retest date. In your post you do not mention and since you have not labeled the chemicals you ask about, I think you may want to check all you labeling. Make sure you are compliant w/ the CAP regulation for labeling and expiration dates for all chemical and reagents in your lab. Just as a precaution and suggestion, to be compliant when you receive any reagent or chemical without a manufacturer expiration date, you must place your own label for receipt and retesting, creating your lab expiration date. The label must have a date for retesting and initialed by the person applying the label. I typically suggest a 12 month period, for patient safety and manageable quality control, to retest the chemical or reagent. Make sure you document your process along w/ the original and retesting results. Also make sure you have labeled any solutions you produce and use in the lab. It is never a good thing for you, the lab or the patient to have an inspector find a container of chemicals or reagents without a label. Check and double check for labels. It is a difficult process to manage and often too easy to find containers without the proper labeling. William DeSalvo, B.S., HTL(ASCP) Production Manager-Anatomic Pathology Chair, NSH Quality Management Committee Owner/Consultant, Collaborative Advantage Consulting > Date: Thu, 31 Jan 2013 15:49:44 -0500 > From: patpxs@gmail.com > To: jpiche-grocki@wtbyhosp.org > Subject: Re: [Histonet] No expiration dates on chemicals > CC: histonet@lists.utsouthwestern.edu > > We keep ours no longer than 5 years. If there is no expiration date > on the bottle when we receive it, we mark it as expired 5 years from > date of receipt. > > Paula > > -- > Paula Sicurello, HTL (ASCP) > Supervisor, Clinical Electron Microscopy Laboratory > Duke University Health System > Rm.#251M, Duke South, Green Zone > Durham, North Carolina 27710 > P: 919.684.2091 > > > On Thu, Jan 31, 2013 at 12:36 PM, PicheGrocki, Jessica > wrote: > > Hi All, > > > > Just wondering what everyone is doing in regards the ANP question involving expiration dates on chemicals and reagents? Is everyone getting rid of old chemicals that have been opened for 10 years or more? > > > > Please advise. > > > > Have a good day and thank you, > > > > Jessica Piche', HT (ASCP) > > Waterbury Hospital Histology Lab > > > > > > > > CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From melissa <@t> alliedsearchpartners.com Thu Jan 31 16:13:32 2013 From: melissa <@t> alliedsearchpartners.com (Melissa Phelan) Date: Thu Jan 31 16:15:21 2013 Subject: [Histonet] Immunohistochemistry Supervisor Message-ID: Hello everyone, Melissa here with ASP. Just an update to let you all know that I have an Immunohistochemistry Supervisor position in New York. Message me for more information. Thank you as always for letting us post our job openings on here! To view a complete list of Allied Search Partners current openings go to: http://www.alliedsearchpartners.com/careers.php Melissa Phelan LinkedIn: http://www.linkedin.com/in/melissaphelan President, Laboratory Staffing Allied Search Partners P: 888.388.7571 ext. 102 F: 888.388.7572 www.alliedsearchpartners.com From tony.henwood <@t> health.nsw.gov.au Thu Jan 31 17:07:25 2013 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Thu Jan 31 17:07:40 2013 Subject: [Histonet] RE: cytoprep In-Reply-To: <3C2378778400AD448ADA6FD6BDB7CCCC179DA5A1@RRMBX03.rrmc.local> References: <906B4DA90ED1DB4DB6C7E94D7CEE6C36220C8FB4@PEITHA.wad.pa-ucl.com> <6D6BD1DE8A5571489398B392A38A71579D22E99E@xmdb04.nch.kids> <3C2378778400AD448ADA6FD6BDB7CCCC179DA5A1@RRMBX03.rrmc.local> Message-ID: <6D6BD1DE8A5571489398B392A38A71579D22ED2E@xmdb04.nch.kids> Joe, The method I believe Nancy is using is based on the ability of saline to re-hydrate air-dried smears prior to PAP staining: Chan and Kung (1988) "Rehydration of air-dried smears with normal saline. Application in fine-needle aspiration cytologic examination".Am J Clin Pathol 89:30-34. Ng WF, Choi FB, Cheung LL, Wu C, Leung CF, Ng CS "Rehydration of air-dried smears with normal saline. Application in fluid cytology" .Acta Cytolog (1994) 38(1):56-64. Jane E. Dahlstrom, Janice Holdsworth, Mark L. Bassett, and Sanjiv Jain, "Rehydration of Air-Dried Smears: An Alternative Method for Cytologic Analysis of Exfoliative Cells" Acta Cytol 1999;43:214-217 Vinod B. Shidham, Bal Kampalath, and James England "Routine Air Drying of All Smears Prepared During Fine Needle Aspiration and Intraoperative Cytology Studies: An Opportunity to Practice a Unified Protocol Offering the Flexibility of Choosing a Variety of Staining Methods" Acta Cytol 2001; 45:60-68 Ganesan Sivaraman and Krishnan R. Iyengar "Rehydrated Air-Dried Pap Smears as an Alternative to Wet-Fixed Smears" Acta Cytol 2002; 46:713-717 Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Joe W. Walker, Jr. [mailto:joewalker@rrmc.org] Sent: Friday, 1 February 2013 2:16 AM To: Tony Henwood (SCHN); 'Nancy Schmitt'; histonet@lists.utsouthwestern.edu Subject: RE: cytoprep I assume that these are conventional Pap tests where the material is smeared onto the slide. I am curious why the slides are air dried upon collection instead of applying a spray fixative to the slide. If these are non-gyn slides from a brushing or FNA, then we might need to have a different discussion. As someone with numerous years experience with cytoprep, I would need more information to help trouble shoot what might be the real issue and to offer suggestions. Cheers, Joe W. Walker, Jr. MS, SCT(ASCP)CM Anatomical Pathology Manager Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P: 802.747.1790 F: 802.747.6525 NEW EMAIL: joewalker@rrmc.org www.rrmc.org Our Vision: To be the Best Community Healthcare System in New England Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet Recognition(r) and the Governor's Award for Performance Excellence -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony Henwood (SCHN) Sent: Wednesday, January 30, 2013 5:05 PM To: 'Nancy Schmitt'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: cytoprep The saline re-hydration works well as long as smears are not allowed to dry too long before rehydration. I have found that air-drying longer than 2 hours decreases the effectiveness of the procedure. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt Sent: Thursday, 31 January 2013 5:18 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cytoprep Hi to all- I am continuing to have a problem with Pap stains done by hand: 1. We rehydrate 25 seconds in saline and straight into alcohol. Thicker areas are ok, but thinner areas appear to be air dried...... 2. Red cell ghosts are NOT clearing I believe there are quite a few Histology labs also performing cytoprep work and I will appreciate any thoughts you may have. Nancy Schmitt HT, MLT Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. Thank You From tony.henwood <@t> health.nsw.gov.au Thu Jan 31 17:39:20 2013 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Thu Jan 31 17:39:30 2013 Subject: [Histonet] Expiration dates of ammonium sulphide Message-ID: <6D6BD1DE8A5571489398B392A38A71579D22EDE0@xmdb04.nch.kids> Hi all, I have a chemical question. We use 20% ammonium sulphide and I am wondering whether it loses its efficiency over time. Is there an expiry date on this solution and is there a test one can do to determine the concentration of aged 20% solutions? I have searched and come up with nuthin! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From tkngflght <@t> yahoo.com Thu Jan 31 19:54:33 2013 From: tkngflght <@t> yahoo.com (Cheryl) Date: Thu Jan 31 19:54:38 2013 Subject: [Histonet] Nice job, great lab Message-ID: <1359683673.36808.YahooMailClassic@web161903.mail.bf1.yahoo.com> Hi All- ? We're seeking a registered histotech with IHC skills (job posting, below).? Please share with your friends--this is the lab I manage and we work hard and have fun doing it!? It's a great crew, awesome docs, and the city is one of those towns that hasn't figured out it's a big city yet--neat things to do without the big city negatives.? ? Job Opening:? Histology Tech II ? Reports to: Histology Manager ? Core Description: Full-time, non-exempt position.? Performs all duties of a certified histology technician in preparing tissue specimens for pathologist examination: grossing, embedding, cutting and staining formalin-fixed tissue in an accurate and timely manner, in accordance with standard histology practices. ? ? Must be a team-player, maintain a cooperative attitude and support on-going changes in a professional manner. ? Schedule: To be determined.? Work schedule may adjust to meet the needs of the department. ? Requirements:? Registered HT or HTL(ASCP). Associates Degree or equivalent with emphasis in science.? Preference given to individuals with 2+ years of IHC experience in a high-volume lab. Bachelor-degreed applicants encouraged to apply. ? Duties include: Embedding, cutting, special stains, immunohistochemistry, troubleshooting, maintenance and support duties. Possible grossing and cytology preparation.? Other duties as assigned to support the operation of the department. Send resumes to kromero@pathgroupla.com? (our HR person)? I will see all the resumes and they're exclusive to this position--no worries about recruiter overload! Cheryl Kerry, HT(ASCP) 800.756.3309?Phone & Fax? admin@fullstaff.org