[Histonet] RESCUING UNDER DECALCIFIED BONE FROM PARAFFIN EMBEDDED BLOCKS

[Craven Peter (NHS HIGHLAND)]

Fiona
We have done this in the past we took the blocks back to water be melting them out, re-cassetteing and putting through a cleaning cycle on the excelsior processor then just decal as normal and reprocess, some hardening often occurs but we can get sections of them suitable for reporting.

Peter

Peter L Craven FIBMS
Pathology Department
Raigmore Hospital
Old Perth Road
Inverness
IV2 3UJ
Tel 01463 704269
email  peter.craven <@t> nhs.net
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Fiona J Morrow [f.morrow <@t> sheffield.ac.uk]
Sent: 28 February 2013 01:59 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] RESCUING UNDER DECALCIFIED BONE FROM PARAFFIN EMBEDDED      BLOCKS

Hi

Has anyone ever tried to reprocess bone tissue that has been under
decalcified and processed through to wax?

Thanks

Fi M


Fiona Morrow
Dept. of Infection and Immunity
KFloor, Room K118
Medical School
University of Sheffield
Beech Hill Road
Sheffield
S10 2RX

0114 271 2102
F.morrow <@t> sheffield.ac.uk

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