[Histonet] RE: Waste Alcohol
Lori White
lwhite38 <@t> cogeco.ca
Thu Feb 28 05:33:36 CST 2013
Our alcohols that cannot be recycled (contaminated with xylene) are combined
with other flammable waste and dealt with by an external company.
L.White
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Sent: Tuesday, February 26, 2013 12:36 PM
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Subject: Histonet Digest, Vol 111, Issue 37
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Today's Topics:
1. Maxvison anti-Rabbit HRP Antibody Substitution (Suresch, Donna L.)
2. RE: Maxvison anti-Rabbit HRP Antibody Substitution (Sarah Dysart)
3. CAP checklist question (Hannen, Valerie)
4. Ventana Labels (Matthew Roark)
5. Re: Ventana Labels (Mark Tarango)
6. RE: Ventana Labels (Bitting, Angela K.)
7. IgG4 (Susan Foreman)
8. Streaking when Cutting (nmhisto <@t> comcast.net)
9. Waste Alcohol (Scott, Allison D)
10. RE: Waste Alcohol (Rathborne, Toni)
11. RE: Waste Alcohol (Horn, Hazel V)
12. RE: Waste Alcohol (Mike Pence)
13. Re: Waste Alcohol (Rene J Buesa)
14. Dako autostainer service (Karla J. C.)
15. RE: CAP checklist question (Susan.Walzer <@t> HCAHealthcare.com)
16. Question for Alkaline Phosphatase / Biotin System Users
(Amy Porter)
17. RE: Waste Alcohol (Horn, Hazel V)
18. RE: Waste Alcohol (Podawiltz, Thomas)
19. Re: Waste Alcohol (Brendal Finlay)
20. streaks (Sheila Fonner)
----------------------------------------------------------------------
Message: 1
Date: Mon, 25 Feb 2013 13:07:02 -0500
From: "Suresch, Donna L." <donna_suresch <@t> merck.com>
Subject: [Histonet] Maxvison anti-Rabbit HRP Antibody Substitution
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
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<2C9A1D9608959940943F357E0A470FF8B2A9D0BC46 <@t> USCTMXP51005.merck.com>
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Hello All,
I have been using Maxvision anti-Rabbit HRP secondary antibody with
excellent results. The product has been discontinued. Has anyone found a
good substitution for this antibody?
Thank you.
Donna L. Suresch
Senior Imaging Research Scientist
Merck Research Laboratories
Department of Imaging - West Point Campus
Mail Stop: WP44K Office: WP44-H129
770 Sumneytown Pike
PO Box 4
West Point, PA 19486-0004
Phone: 215-652-7349
Fax: 215-993-6803
Notice: This e-mail message, together with any attachments, contains
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------------------------------
Message: 2
Date: Mon, 25 Feb 2013 18:15:20 +0000
From: Sarah Dysart <sdysart <@t> mirnarx.com>
Subject: [Histonet] RE: Maxvison anti-Rabbit HRP Antibody Substitution
To: "Suresch, Donna L." <donna_suresch <@t> merck.com>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<8A70A9B2ECDD084DACFE6C59FCF86D50485058B1 <@t> BL2PRD0711MB434.namprd07.prod.outl
ook.com>
Content-Type: text/plain; charset="us-ascii"
The Biocare polymers (MACH2 series) are excellent!!
Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna
Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas 78744
(512)901-0900 ext. 6912
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Suresch,
Donna L.
Sent: Monday, February 25, 2013 12:07 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Maxvison anti-Rabbit HRP Antibody Substitution
Hello All,
I have been using Maxvision anti-Rabbit HRP secondary antibody with
excellent results. The product has been discontinued. Has anyone found a
good substitution for this antibody?
Thank you.
Donna L. Suresch
Senior Imaging Research Scientist
Merck Research Laboratories
Department of Imaging - West Point Campus
Mail Stop: WP44K Office: WP44-H129
770 Sumneytown Pike
PO Box 4
West Point, PA 19486-0004
Phone: 215-652-7349
Fax: 215-993-6803
Notice: This e-mail message, together with any attachments, contains
information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New
Jersey, USA 08889), and/or its affiliates Direct contact information for
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http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely for
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------------------------------
Message: 3
Date: Mon, 25 Feb 2013 13:21:16 -0500
From: "Hannen, Valerie" <Valerie.Hannen <@t> parrishmed.com>
Subject: [Histonet] CAP checklist question
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <450B7A81EDA0C54E97C53D60F00776C3231B3D7BEC <@t> isexstore03>
Content-Type: text/plain; charset="us-ascii"
Hi gang..
I am working on a self-inspection CAP checklist and came across the
following:
ANP.23120 Tissue Processor
Tissue processing schedules are validated. Note: New
tissue processing schedules must be validated against the standard
laboratory processing schedule.
Evidence of compliance: Written procedure for validation of new tissue
processing schedules AND
WC records documenting validation
What do you all make of this?? We have not had any new processing schedules
introduced into our lab in years. So, I really don't know how to proceed on
this one.
Any insite will be greatly appreciated.
Valerie A. Hannen, MLT(ASCP),HTL,SU(FL)
Histology Section Chief
Parrish Medical Center
951 N. Washington Ave.
Titusville, Florida 32976
Phone:(321) 268-6333 ext. 7506
Fax: (321) 268-6149
valerie.hannen <@t> parrishmed.com
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------------------------------
Message: 4
Date: Mon, 25 Feb 2013 13:33:55 -0600
From: "Matthew Roark" <mroark <@t> sfmc.net>
Subject: [Histonet] Ventana Labels
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <002101ce138f$0cf92a30$26eb7e90$@net>
Content-Type: text/plain; charset="us-ascii"
Hello all,
I am getting ready to try some slide labels as an alternative to the Ventana
labels. I was wondering if anyone else is using them or have tried them in
the past.
They do not have that plastic fold over flap and are suppose to be quite
economical, though I have not got a quote yet.
They are called FloProTek (http://www.easternlabsvc.com/floprotek.php) -
Mercedes Medical Supply sent me the trial roll.
Thanks!
Matthew Roark- HT/HTL(ASCP)CM
Histology Specialist
Saint Francis Medical Center
211 Saint Francis Drive
Cape Girardeau, MO 63703
573-331-5267
mroark <@t> sfmc.net
http://www.sfmc.net
------------------------------
Message: 5
Date: Mon, 25 Feb 2013 11:43:38 -0800
From: Mark Tarango <marktarango <@t> gmail.com>
Subject: Re: [Histonet] Ventana Labels
To: Matthew Roark <mroark <@t> sfmc.net>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<CACJwj1K6NiDPJX8m=QAkbqqPmyhb+PaaEAeyjqEMC_pRvjuxQw <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Hi Matthew,
We use them and they work fine. They are much cheaper too. We've had them
for about 3 years. I initially had some concerns about the DAB being
absorbed and staining the labels on some cases. Our safety person told me
that it's not a safety concern and it's safe to touch the label after it's
stained brown by DAB. This doesn't happen on every slide but is a random
thing. It might have something to do with how well it was affixed to the
slide.
The labels don't affect the staining in my experience.
Mark
On Mon, Feb 25, 2013 at 11:33 AM, Matthew Roark <mroark <@t> sfmc.net> wrote:
> Hello all,
>
> I am getting ready to try some slide labels as an alternative to the
> Ventana labels. I was wondering if anyone else is using them or have
> tried them in the past.
>
> They do not have that plastic fold over flap and are suppose to be
> quite economical, though I have not got a quote yet.
>
> They are called FloProTek (http://www.easternlabsvc.com/floprotek.php)
> - Mercedes Medical Supply sent me the trial roll.
>
> Thanks!
>
>
> Matthew Roark- HT/HTL(ASCP)CM
> Histology Specialist
> Saint Francis Medical Center
> 211 Saint Francis Drive
> Cape Girardeau, MO 63703
> 573-331-5267
> mroark <@t> sfmc.net
> http://www.sfmc.net
>
>
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
------------------------------
Message: 6
Date: Mon, 25 Feb 2013 19:55:59 +0000
From: "Bitting, Angela K." <akbitting <@t> geisinger.edu>
Subject: RE: [Histonet] Ventana Labels
To: Matthew Roark <mroark <@t> sfmc.net>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<77F52EFAB8B1694B885E277C48FCD0F6328503A5 <@t> GHSEXMBX4W8K1V.geisinger.edu>
Content-Type: text/plain; charset="us-ascii"
I just tried them Friday. They worked fine but the label picked up
counterstain a bit. However, depending on the cost per label, it may be
worth the trouble. I'm waiting for a quote.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Matthew
Roark
Sent: Monday, February 25, 2013 2:34 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Ventana Labels
Hello all,
I am getting ready to try some slide labels as an alternative to the Ventana
labels. I was wondering if anyone else is using them or have tried them in
the past.
They do not have that plastic fold over flap and are suppose to be quite
economical, though I have not got a quote yet.
They are called FloProTek (http://www.easternlabsvc.com/floprotek.php) -
Mercedes Medical Supply sent me the trial roll.
Thanks!
Matthew Roark- HT/HTL(ASCP)CM
Histology Specialist
Saint Francis Medical Center
211 Saint Francis Drive
Cape Girardeau, MO 63703
573-331-5267
mroark <@t> sfmc.net
http://www.sfmc.net
_______________________________________________
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Message: 7
Date: Mon, 25 Feb 2013 14:57:25 -0500
From: "Susan Foreman" <sforeman <@t> labpath.com>
Subject: [Histonet] IgG4
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <002e01ce1392$554fa900$ffeefb00$@com>
Content-Type: text/plain; charset="us-ascii"
Has anyone worked up IgG4 on the Ventana Benchmark Ultra? What vendor?
How about running it on fresh frozen sections? FITC? Vendor?
Many Thanks for your input,
Susan
------------------------------
Message: 8
Date: Mon, 25 Feb 2013 20:09:08 +0000 (UTC)
From: nmhisto <@t> comcast.net
Subject: [Histonet] Streaking when Cutting
To: HISTONET <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<1822368949.1502216.1361822948867.JavaMail.root <@t> sz0075a.emeryville.ca.mail.c
omcast.net>
Content-Type: text/plain; charset=utf-8
I've been quietly observing from the sidelines, but I just can't pass this
one up.B One should never streak when cutting - it's distracting to fellow
techs.B Okay - back to my nap.
------------------------------
Message: 9
Date: Mon, 25 Feb 2013 20:27:55 +0000
From: "Scott, Allison D" <Allison.Scott <@t> harrishealth.org>
Subject: [Histonet] Waste Alcohol
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<FA607DC3D1ED7C46A9546820A3EB877F0A1E197F <@t> LBMSG02.hchd.local>
Content-Type: text/plain; charset="us-ascii"
Hello to all in histoland. Is there anyone putting alcohol into a waste
drum like for xylene. We have a company that takes away our xylene. Now
the safety people are suggesting that we treat the alcohol the same way. We
have always poured the alcohol down the drain. It seems very expensive.
Your help in this matter will be greatly appreciated.
Allison Scott HT(ASCP)
Supervisor, Histology Lab
LBJ Hospital
Harris Health System
Office: 713-566-5287
Lab: 713-566-5287
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Message: 10
Date: Mon, 25 Feb 2013 20:33:27 +0000
From: "Rathborne, Toni" <trathborne <@t> somerset-healthcare.com>
Subject: [Histonet] RE: Waste Alcohol
To: "'Scott, Allison D'" <Allison.Scott <@t> harrishealth.org>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<3AD061FE740D464FAC7BF6B5CFB75707599DF5C3 <@t> smcmail02.somerset-healthcare.com>
Content-Type: text/plain; charset="us-ascii"
We have been disposing of our alcohols like this for years. This would
include alcoholic eosin too. We are allowed to mix the waste xylene with the
alcohols because they are the same classification, and treated the same way.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Scott,
Allison D
Sent: Monday, February 25, 2013 3:28 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Waste Alcohol
Hello to all in histoland. Is there anyone putting alcohol into a waste
drum like for xylene. We have a company that takes away our xylene. Now
the safety people are suggesting that we treat the alcohol the same way. We
have always poured the alcohol down the drain. It seems very expensive.
Your help in this matter will be greatly appreciated.
Allison Scott HT(ASCP)
Supervisor, Histology Lab
LBJ Hospital
Harris Health System
Office: 713-566-5287
Lab: 713-566-5287
CONFIDENTIALITY NOTICE:
If you have received this e-mail in error, please immediately notify the
sender by return e-mail and delete this e-mail and any attachments from your
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To the extent the information in this e-mail and any attachments contain
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and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and
164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or
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------------------------------
Message: 11
Date: Mon, 25 Feb 2013 15:02:10 -0600
From: "Horn, Hazel V" <HornHV <@t> archildrens.org>
Subject: [Histonet] RE: Waste Alcohol
To: "'Scott, Allison D'" <Allison.Scott <@t> harrishealth.org>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<25A4DE08332B19499904459F00AAACB719BE135634 <@t> EVS1.archildrens.org>
Content-Type: text/plain; charset="us-ascii"
Our xylene and alcohol go into a waste drum to be disposed of. We do
recycle both but of course we still have waste to dispose of.
Hazel Horn
Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas
Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hornhv <@t> archildrens.org
archildrens.org
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Scott,
Allison D
Sent: Monday, February 25, 2013 2:28 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Waste Alcohol
Hello to all in histoland. Is there anyone putting alcohol into a waste
drum like for xylene. We have a company that takes away our xylene. Now
the safety people are suggesting that we treat the alcohol the same way. We
have always poured the alcohol down the drain. It seems very expensive.
Your help in this matter will be greatly appreciated.
Allison Scott HT(ASCP)
Supervisor, Histology Lab
LBJ Hospital
Harris Health System
Office: 713-566-5287
Lab: 713-566-5287
CONFIDENTIALITY NOTICE:
If you have received this e-mail in error, please immediately notify the
sender by return e-mail and delete this e-mail and any attachments from your
computer system.
To the extent the information in this e-mail and any attachments contain
protected health information as defined by the Health Insurance Portability
and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and
164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or
privileged. This e-mail may also be confidential and/or privileged under
Texas law. The e-mail is for the use of only the individual or entity named
above. If you are not the intended recipient, or any authorized
representative of the intended recipient, you are hereby notified that any
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_______________________________________________
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Message: 12
Date: Mon, 25 Feb 2013 21:18:12 +0000
From: Mike Pence <mpence <@t> grhs.net>
Subject: [Histonet] RE: Waste Alcohol
To: "Scott, Allison D" <Allison.Scott <@t> harrishealth.org>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <D6DF96C438FB8D42B04B2345A9452CF5AEDB <@t> MAIL-MB01.grhs.net>
Content-Type: text/plain; charset="us-ascii"
You should be recycling both xylene and alcohol. Cost savings compared to
disposal cost will pay for a recycler in 3-5 years.
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu
[histonet-bounces <@t> lists.utsouthwestern.edu] on behalf of Scott, Allison D
[Allison.Scott <@t> harrishealth.org]
Sent: Monday, February 25, 2013 2:27 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Waste Alcohol
Hello to all in histoland. Is there anyone putting alcohol into a waste
drum like for xylene. We have a company that takes away our xylene. Now
the safety people are suggesting that we treat the alcohol the same way. We
have always poured the alcohol down the drain. It seems very expensive.
Your help in this matter will be greatly appreciated.
Allison Scott HT(ASCP)
Supervisor, Histology Lab
LBJ Hospital
Harris Health System
Office: 713-566-5287
Lab: 713-566-5287
CONFIDENTIALITY NOTICE:
If you have received this e-mail in error, please immediately notify the
sender by return e-mail and delete this e-mail and any attachments from your
computer system.
To the extent the information in this e-mail and any attachments contain
protected health information as defined by the Health Insurance Portability
and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and
164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or
privileged. This e-mail may also be confidential and/or privileged under
Texas law. The e-mail is for the use of only the individual or entity named
above. If you are not the intended recipient, or any authorized
representative of the intended recipient, you are hereby notified that any
review, dissemination or copying of this e-mail and its attachments is
strictly prohibited.
_______________________________________________
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------------------------------
Message: 13
Date: Mon, 25 Feb 2013 13:53:22 -0800 (PST)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Waste Alcohol
To: "Scott, Allison D" <Allison.Scott <@t> harrishealth.org>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<1361829202.27805.YahooMailNeo <@t> web163104.mail.bf1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
I always dumped the ethanol down the drain. I never recycled it because it
takes almost 3 times more time that recycling xylene (3 times the cost) and
the recycled alcohol is seldom of good quality.
When I stopped using xylene I had no more use (and exposure) when recycling.
Reni J.
From: "Scott, Allison D" <Allison.Scott <@t> harrishealth.org>
To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
Sent: Monday, February 25, 2013 3:27 PM
Subject: [Histonet] Waste Alcohol
Hello to all in histoland. Is there anyone putting alcohol into a waste
drum like for xylene. We have a company that takes away our xylene. Now
the safety people are suggesting that we treat the alcohol the same way. We
have always poured the alcohol down the drain. It seems very expensive.
Your help in this matter will be greatly appreciated.
Allison Scott HT(ASCP)
Supervisor, Histology Lab
LBJ Hospital
Harris Health System
Office: 713-566-5287
Lab: 713-566-5287
CONFIDENTIALITY NOTICE:
If you have received this e-mail in error, please immediately notify the
sender by return e-mail and delete this e-mail and any attachments from your
computer system.
To the extent the information in this e-mail and any attachments contain
protected health information as defined by the Health Insurance Portability
and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and
164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or
privileged. This e-mail may also be confidential and/or privileged under
Texas law. The e-mail is for the use of only the individual or entity named
above. If you are not the intended recipient, or any authorized
representative of the intended recipient, you are hereby notified that any
review, dissemination or copying of this e-mail and its attachments is
strictly prohibited.
_______________________________________________
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Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 14
Date: Mon, 25 Feb 2013 13:47:34 -0800
From: "Karla J. C." <karla <@t> dermatopathologynorthwest.com>
Subject: [Histonet] Dako autostainer service
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<000f01ce13a1$b8de93a0$2a9bbae0$@dermatopathologynorthwest.com>
Content-Type: text/plain; charset="us-ascii"
Hi All,
Help.Dako just recently informed us that they would no longer provide a
Service contract for our immuno autostainer. Is anyone else experiencing
this? Does anyone know of any other service people that may be able to work
on this equipment (model 3400 year 2003)? We are located in Seattle.
Thanks,
Karla Carlmas
Dermatopathology Northwest
------------------------------
Message: 15
Date: Tue, 26 Feb 2013 01:55:58 -0600
From: <Susan.Walzer <@t> HCAHealthcare.com>
Subject: [Histonet] RE: CAP checklist question
To: <Valerie.Hannen <@t> parrishmed.com>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<4BF03F5404EBDE409AF9232DA74B9DED2DFF10BF41 <@t> FWDCWPMSGCMS09.hca.corpad.net>
Content-Type: text/plain; charset="us-ascii"
We put in a procedure stating that should we change the schedule we will
test a variety of tissue (10 different types) and have the pathologist
assess the run. We have not changed a schedule in years but we seek to
comply...
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Hannen,
Valerie
Sent: Monday, February 25, 2013 1:21 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] CAP checklist question
Hi gang..
I am working on a self-inspection CAP checklist and came across the
following:
ANP.23120 Tissue Processor
Tissue processing schedules are validated. Note: New
tissue processing schedules must be validated against the standard
laboratory processing schedule.
Evidence of compliance: Written procedure for validation of new tissue
processing schedules AND
WC records documenting validation
What do you all make of this?? We have not had any new processing schedules
introduced into our lab in years. So, I really don't know how to proceed on
this one.
Any insite will be greatly appreciated.
Valerie A. Hannen, MLT(ASCP),HTL,SU(FL)
Histology Section Chief
Parrish Medical Center
951 N. Washington Ave.
Titusville, Florida 32976
Phone:(321) 268-6333 ext. 7506
Fax: (321) 268-6149
valerie.hannen <@t> parrishmed.com
=============
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------------------------------
Message: 16
Date: Tue, 26 Feb 2013 09:14:28 -0500
From: "Amy Porter" <portera <@t> msu.edu>
Subject: [Histonet] Question for Alkaline Phosphatase / Biotin System
Users
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <000d01ce142b$96d73ac0$c485b040$@edu>
Content-Type: text/plain; charset="us-ascii"
We are using standard avidin / biotin staining with alkaline phosphatase
labeling and sometimes, not every time and not every slide in the same run
we get residual blotchy spots and streaking of the reaction on and off the
tissue. We have increased to two rinses after primary and two rinses after
alk phos reagent prior to substrate; still happening on some slides...anyone
else have this experience or type of problem? Any experiences or
suggestions are appreciated. Just can't figure out what is causing it..
Amy S. Porter, HT(ASCP) QIHC
Michigan State University
Investigative HistoPathology Laboratory
portera <@t> msu.edu
------------------------------
Message: 17
Date: Tue, 26 Feb 2013 09:27:03 -0600
From: "Horn, Hazel V" <HornHV <@t> archildrens.org>
Subject: RE: [Histonet] Waste Alcohol
To: "'Rene J Buesa'" <rjbuesa <@t> yahoo.com>, "Scott, Allison D"
<Allison.Scott <@t> harrishealth.org>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<25A4DE08332B19499904459F00AAACB719BE13563A <@t> EVS1.archildrens.org>
Content-Type: text/plain; charset="iso-8859-1"
The ability to put alcohol down the drain is regulated by your wastewater
utility.
Hazel Horn
Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas
Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hornhv <@t> archildrens.org
archildrens.org
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Monday, February 25, 2013 3:53 PM
To: Scott, Allison D; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Waste Alcohol
I always dumped the ethanol down the drain. I never recycled it because it
takes almost 3 times more time that recycling xylene (3 times the cost) and
the recycled alcohol is seldom of good quality.
When I stopped using xylene I had no more use (and exposure) when recycling.
Reni J.
From: "Scott, Allison D" <Allison.Scott <@t> harrishealth.org>
To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
Sent: Monday, February 25, 2013 3:27 PM
Subject: [Histonet] Waste Alcohol
Hello to all in histoland. Is there anyone putting alcohol into a waste
drum like for xylene. We have a company that takes away our xylene. Now
the safety people are suggesting that we treat the alcohol the same way. We
have always poured the alcohol down the drain. It seems very expensive.
Your help in this matter will be greatly appreciated.
Allison Scott HT(ASCP)
Supervisor, Histology Lab
LBJ Hospital
Harris Health System
Office: 713-566-5287
Lab: 713-566-5287
CONFIDENTIALITY NOTICE:
If you have received this e-mail in error, please immediately notify the
sender by return e-mail and delete this e-mail and any attachments from your
computer system.
To the extent the information in this e-mail and any attachments contain
protected health information as defined by the Health Insurance Portability
and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and
164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or
privileged. This e-mail may also be confidential and/or privileged under
Texas law. The e-mail is for the use of only the individual or entity named
above. If you are not the intended recipient, or any authorized
representative of the intended recipient, you are hereby notified that any
review, dissemination or copying of this e-mail and its attachments is
strictly prohibited.
_______________________________________________
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Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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The information contained in this message may be privileged and confidential
and protected from disclosure. If the reader of this message is not the
intended recipient, or an employee or agent responsible for delivering this
message to the intended recipient, you are hereby notified that any
dissemination, distribution or copying of this communication is strictly
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Thank you.
------------------------------
Message: 18
Date: Tue, 26 Feb 2013 10:55:14 -0500
From: "Podawiltz, Thomas" <tpodawiltz <@t> lrgh.org>
Subject: RE: [Histonet] Waste Alcohol
To: "Horn, Hazel V" <HornHV <@t> archildrens.org>, 'Rene J Buesa'
<rjbuesa <@t> yahoo.com>, "Scott, Allison D"
<Allison.Scott <@t> harrishealth.org>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<38667E7FB77ECD4E91BFAEB8D986386324FCF3C6D9 <@t> LRGHEXVS1.practice.lrgh.org>
Content-Type: text/plain; charset="iso-8859-1"
Reagent Alcohol contains methanol, which is a U-listed EPA hazardous
chemical and cannot be poured down the drain.
Tom Podawiltz HT (ASCP)
Histology Section Head/Laboratory Safety Officer.
LRGHealthcare
Laconia, NH 03246
603-524-3211 ext: 3220
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Horn, Hazel
V
Sent: Tuesday, February 26, 2013 10:27 AM
To: 'Rene J Buesa'; Scott, Allison D; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Waste Alcohol
The ability to put alcohol down the drain is regulated by your wastewater
utility.
Hazel Horn
Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas
Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hornhv <@t> archildrens.org
archildrens.org
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Monday, February 25, 2013 3:53 PM
To: Scott, Allison D; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Waste Alcohol
I always dumped the ethanol down the drain. I never recycled it because it
takes almost 3 times more time that recycling xylene (3 times the cost) and
the recycled alcohol is seldom of good quality.
When I stopped using xylene I had no more use (and exposure) when recycling.
Reni J.
From: "Scott, Allison D" <Allison.Scott <@t> harrishealth.org>
To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
Sent: Monday, February 25, 2013 3:27 PM
Subject: [Histonet] Waste Alcohol
Hello to all in histoland. Is there anyone putting alcohol into a waste
drum like for xylene. We have a company that takes away our xylene. Now
the safety people are suggesting that we treat the alcohol the same way. We
have always poured the alcohol down the drain. It seems very expensive.
Your help in this matter will be greatly appreciated.
Allison Scott HT(ASCP)
Supervisor, Histology Lab
LBJ Hospital
Harris Health System
Office: 713-566-5287
Lab: 713-566-5287
CONFIDENTIALITY NOTICE:
If you have received this e-mail in error, please immediately notify the
sender by return e-mail and delete this e-mail and any attachments from your
computer system.
To the extent the information in this e-mail and any attachments contain
protected health information as defined by the Health Insurance Portability
and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and
164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or
privileged. This e-mail may also be confidential and/or privileged under
Texas law. The e-mail is for the use of only the individual or entity named
above. If you are not the intended recipient, or any authorized
representative of the intended recipient, you are hereby notified that any
review, dissemination or copying of this e-mail and its attachments is
strictly prohibited.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
****************************************************************************
****************************************************************************
****************************************************************************
****************************************************************************
****************************************************************************
****************************************************************************
****************************************************************************
****************************************************************************
**********************
The information contained in this message may be privileged and confidential
and protected from disclosure. If the reader of this message is not the
intended recipient, or an employee or agent responsible for delivering this
message to the intended recipient, you are hereby notified that any
dissemination, distribution or copying of this communication is strictly
prohibited. If you have received this communication in error, please notify
us immediately by replying to the message and deleting it from your
computer.
Thank you.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
THIS MESSAGE IS CONFIDENTIAL.
This e-mail message and any attachments are proprietary and confidential
information intended only for the use of the recipient(s) named above. If
you are not the intended recipient, you may not print,distribute, or copy
this message or any attachments. If you have received this communication in
error, please notify the sender by return e-mail and delete this message and
any attachments from your computer. Any views or opinions expressed are
solely those of the author and do not necessarily represent those of
LRGHealthcare.
------------------------------
Message: 19
Date: Tue, 26 Feb 2013 09:56:12 -0600
From: "Brendal Finlay" <brendal.finlay <@t> medicalcenterclinic.com>
Subject: Re: [Histonet] Waste Alcohol
To: "Rene J Buesa" <rjbuesa <@t> yahoo.com>, "Scott, Allison D"
<Allison.Scott <@t> harrishealth.org>,
histonet <@t> lists.utsouthwestern.edu
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <4fcdbb6eed8fb2fc1e5ea8e615ce00ee <@t> medicalcenterclinic.com>
Content-Type: text/plain; charset="utf-8"
YouB will probably want to contact your local or state office of
environmental protection to find out what is required in your area.B
B
The EPA lists hazardous wastes in 40 CFR 261.30.B Even if your waste is not
on this list, it may have hazardous characteristics such as:
ignitability, corrosivity, reactivity, and toxicity characteristic.B
Ignitability pertains to flashpoints less than 140 F and/or aqueous
solutions with alcohol content greater than or equal to 24 percent.B
B
Florida's DEP has different regulations dependent upon the quantity of
hazardous waste generated.B Some localities may require approval to
dispose of alcohol down the drain.B If you don't have this approval,
you have to dispose of the waste according toB your
stateB andB federalregulations.B
B
To reduce the quantity of hazardous waste we generate, we began to
recycle using CBG Biotech's distillation unit.B We have had great
results with it.B While alcohol does take longer to recycle than
xylene, our supply costs have been greatly reduced and we have worked
this into our weekly schedule very well.
B
Some things to consider when using recycled alcohol:
Test each finished alcohol run with a hydrometer to know what
percentage it is.B
Do not use recycled alcohol at the end of the stain line after eosin
(it will wash the eosin out).
Do not use recycled alcohol as the last alcohol before xylene on the
processor.
Finally, do not recycle the first alcohols on the stain line after
xylene (at least on our recycler). This is disposed of in our
flammable waste containers to be picked up.
B
Hereb
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