[Histonet] polarizing microscopy

[Rene J Buesa]

The best and less expensive solution is to go to a photography store and buy a plastic polarizing filter.
Cut a round piece and place it in the condenser filter ring and cut another (of much smaller diameter) plastic filter and place it in the filter holder of your microscope ocular.
The light getting into the specimen coming from the condenser will be partially polarized and moving the ocular in a circular manner, you will obtain the polarization of the field of view and the specimen.
René J.

From: Salomao Segal <ssegal2 <@t> slu.edu>
To: histonet <@t> lists.utsouthwestern.edu 
Sent: Wednesday, February 27, 2013 11:30 AM
Subject: [Histonet] polarizing microscopy

I own an old Zeiss microscope (Universal) and would like to have the
capability of performing polarized light microscopy studies.

I am particularly interested in exploiting the birefringent properties of
myelinated fibers in peripheral nerves.

My questions are:

1) what are the microscope parts needed for polarizing microscopy?
(condenser, filters, stage, objectives, etc.)

2) any good source about these techniques for the beginner?

Any guidance would be greatly appreciated.

Thanks

-- 
Solomon Segal, M.D.
Assistant Professor of Anatomy in Surgery
Center for Anatomical Science and Education (CASE)
Department of Surgery
School of Medicine
Saint Louis University

1402 South Grand Blvd.
Schwitalla Hall - 3rd Floor - M310
Saint Louis, MO, 63104
office: 314 977 8023
laboratory: 314 977 8080
CASE: 314 977 8027
FAX: 314 977 5127
e-mail: ssegal2 <@t> slu.edu

http://medschool.slu.edu/anatomy/

http://slu.academia.edu/SolomonSegal

https://sites.google.com/a/slu.edu/segal-laboratory/

https://sites.google.com/a/slu.edu/dr-segal-s-clinical-anatomy-website/
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