[Histonet] RE: Histonet Digest, Vol 111, Issue 37
Erik Dokken
Erik.Dokken <@t> onassignment.com
Tue Feb 26 15:53:36 CST 2013
Good afternoon Histonetters!
We are currently looking for a qualified Pathology Assistant (ASCP Cert Required) for a full time position with a lab in Palo Alto, CA.
Job description below;
The Pathologists' Assistant (PA) dissects tissue specimens and provides dictated placement in plastic cassettes for embedding, staining, and microscopic analysis. This position directs coding of specimens for the accessioning staff. Processes breast and other tissue specimens according to established protocols, and instructs residents, fellows, medical students, and faculty in Gross Room protocols and processes as well as specimen photography. This position is responsible for responding on a STAT basis to the frozen section room adjacent to the SHC operating rooms, and performs intraoperative frozen sections. The PA is also responsible for assembling catalogues and preserving specimens for the gross specimen teaching library, and the Department of Pathology Tissue Bank. This position prepares and performs specimen radiography of breast, bone, and soft tissue specimens.
ASCP certification as a Pathology Assistant required.
If you know of anyone looking for work in the Bay Area we would greatly appreciate the referral.
Thank you again,
Erik Dokken
Erik.dokken <@t> onassignment.com
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Tuesday, February 26, 2013 9:36 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 111, Issue 37
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Today's Topics:
1. Maxvison anti-Rabbit HRP Antibody Substitution (Suresch, Donna L.)
2. RE: Maxvison anti-Rabbit HRP Antibody Substitution (Sarah Dysart)
3. CAP checklist question (Hannen, Valerie)
4. Ventana Labels (Matthew Roark)
5. Re: Ventana Labels (Mark Tarango)
6. RE: Ventana Labels (Bitting, Angela K.)
7. IgG4 (Susan Foreman)
8. Streaking when Cutting (nmhisto <@t> comcast.net)
9. Waste Alcohol (Scott, Allison D)
10. RE: Waste Alcohol (Rathborne, Toni)
11. RE: Waste Alcohol (Horn, Hazel V)
12. RE: Waste Alcohol (Mike Pence)
13. Re: Waste Alcohol (Rene J Buesa)
14. Dako autostainer service (Karla J. C.)
15. RE: CAP checklist question (Susan.Walzer <@t> HCAHealthcare.com)
16. Question for Alkaline Phosphatase / Biotin System Users
(Amy Porter)
17. RE: Waste Alcohol (Horn, Hazel V)
18. RE: Waste Alcohol (Podawiltz, Thomas)
19. Re: Waste Alcohol (Brendal Finlay)
20. streaks (Sheila Fonner)
----------------------------------------------------------------------
Message: 1
Date: Mon, 25 Feb 2013 13:07:02 -0500
From: "Suresch, Donna L." <donna_suresch <@t> merck.com>
Subject: [Histonet] Maxvison anti-Rabbit HRP Antibody Substitution
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<2C9A1D9608959940943F357E0A470FF8B2A9D0BC46 <@t> USCTMXP51005.merck.com>
Content-Type: text/plain; charset="us-ascii"
Hello All,
I have been using Maxvision anti-Rabbit HRP secondary antibody with excellent results. The product has been discontinued. Has anyone found a good substitution for this antibody?
Thank you.
Donna L. Suresch
Senior Imaging Research Scientist
Merck Research Laboratories
Department of Imaging - West Point Campus
Mail Stop: WP44K Office: WP44-H129
770 Sumneytown Pike
PO Box 4
West Point, PA 19486-0004
Phone: 215-652-7349
Fax: 215-993-6803
Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at
http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system.
------------------------------
Message: 2
Date: Mon, 25 Feb 2013 18:15:20 +0000
From: Sarah Dysart <sdysart <@t> mirnarx.com>
Subject: [Histonet] RE: Maxvison anti-Rabbit HRP Antibody Substitution
To: "Suresch, Donna L." <donna_suresch <@t> merck.com>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<8A70A9B2ECDD084DACFE6C59FCF86D50485058B1 <@t> BL2PRD0711MB434.namprd07.prod.outlook.com>
Content-Type: text/plain; charset="us-ascii"
The Biocare polymers (MACH2 series) are excellent!!
Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas 78744
(512)901-0900 ext. 6912
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Suresch, Donna L.
Sent: Monday, February 25, 2013 12:07 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Maxvison anti-Rabbit HRP Antibody Substitution
Hello All,
I have been using Maxvision anti-Rabbit HRP secondary antibody with excellent results. The product has been discontinued. Has anyone found a good substitution for this antibody?
Thank you.
Donna L. Suresch
Senior Imaging Research Scientist
Merck Research Laboratories
Department of Imaging - West Point Campus
Mail Stop: WP44K Office: WP44-H129
770 Sumneytown Pike
PO Box 4
West Point, PA 19486-0004
Phone: 215-652-7349
Fax: 215-993-6803
Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at
http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system.
_______________________________________________
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Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 3
Date: Mon, 25 Feb 2013 13:21:16 -0500
From: "Hannen, Valerie" <Valerie.Hannen <@t> parrishmed.com>
Subject: [Histonet] CAP checklist question
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <450B7A81EDA0C54E97C53D60F00776C3231B3D7BEC <@t> isexstore03>
Content-Type: text/plain; charset="us-ascii"
Hi gang..
I am working on a self-inspection CAP checklist and came across the following:
ANP.23120 Tissue Processor
Tissue processing schedules are validated. Note: New tissue processing schedules must be validated against the standard laboratory processing schedule.
Evidence of compliance: Written procedure for validation of new tissue processing schedules AND
WC records documenting validation
What do you all make of this?? We have not had any new processing schedules introduced into our lab in years. So, I really don't know how to proceed on this one.
Any insite will be greatly appreciated.
Valerie A. Hannen, MLT(ASCP),HTL,SU(FL)
Histology Section Chief
Parrish Medical Center
951 N. Washington Ave.
Titusville, Florida 32976
Phone:(321) 268-6333 ext. 7506
Fax: (321) 268-6149
valerie.hannen <@t> parrishmed.com
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------------------------------
Message: 4
Date: Mon, 25 Feb 2013 13:33:55 -0600
From: "Matthew Roark" <mroark <@t> sfmc.net>
Subject: [Histonet] Ventana Labels
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <002101ce138f$0cf92a30$26eb7e90$@net>
Content-Type: text/plain; charset="us-ascii"
Hello all,
I am getting ready to try some slide labels as an alternative to the Ventana labels. I was wondering if anyone else is using them or have tried them in the past.
They do not have that plastic fold over flap and are suppose to be quite economical, though I have not got a quote yet.
They are called FloProTek (http://www.easternlabsvc.com/floprotek.php) - Mercedes Medical Supply sent me the trial roll.
Thanks!
Matthew Roark- HT/HTL(ASCP)CM
Histology Specialist
Saint Francis Medical Center
211 Saint Francis Drive
Cape Girardeau, MO 63703
573-331-5267
mroark <@t> sfmc.net
http://www.sfmc.net
------------------------------
Message: 5
Date: Mon, 25 Feb 2013 11:43:38 -0800
From: Mark Tarango <marktarango <@t> gmail.com>
Subject: Re: [Histonet] Ventana Labels
To: Matthew Roark <mroark <@t> sfmc.net>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<CACJwj1K6NiDPJX8m=QAkbqqPmyhb+PaaEAeyjqEMC_pRvjuxQw <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Hi Matthew,
We use them and they work fine. They are much cheaper too. We've had them for about 3 years. I initially had some concerns about the DAB being absorbed and staining the labels on some cases. Our safety person told me that it's not a safety concern and it's safe to touch the label after it's stained brown by DAB. This doesn't happen on every slide but is a random thing. It might have something to do with how well it was affixed to the slide.
The labels don't affect the staining in my experience.
Mark
On Mon, Feb 25, 2013 at 11:33 AM, Matthew Roark <mroark <@t> sfmc.net> wrote:
> Hello all,
>
> I am getting ready to try some slide labels as an alternative to the
> Ventana labels. I was wondering if anyone else is using them or have
> tried them in the past.
>
> They do not have that plastic fold over flap and are suppose to be
> quite economical, though I have not got a quote yet.
>
> They are called FloProTek (http://www.easternlabsvc.com/floprotek.php)
> - Mercedes Medical Supply sent me the trial roll.
>
> Thanks!
>
>
> Matthew Roark- HT/HTL(ASCP)CM
> Histology Specialist
> Saint Francis Medical Center
> 211 Saint Francis Drive
> Cape Girardeau, MO 63703
> 573-331-5267
> mroark <@t> sfmc.net
> http://www.sfmc.net
>
>
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
------------------------------
Message: 6
Date: Mon, 25 Feb 2013 19:55:59 +0000
From: "Bitting, Angela K." <akbitting <@t> geisinger.edu>
Subject: RE: [Histonet] Ventana Labels
To: Matthew Roark <mroark <@t> sfmc.net>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<77F52EFAB8B1694B885E277C48FCD0F6328503A5 <@t> GHSEXMBX4W8K1V.geisinger.edu>
Content-Type: text/plain; charset="us-ascii"
I just tried them Friday. They worked fine but the label picked up counterstain a bit. However, depending on the cost per label, it may be worth the trouble. I'm waiting for a quote.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Matthew Roark
Sent: Monday, February 25, 2013 2:34 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Ventana Labels
Hello all,
I am getting ready to try some slide labels as an alternative to the Ventana labels. I was wondering if anyone else is using them or have tried them in the past.
They do not have that plastic fold over flap and are suppose to be quite economical, though I have not got a quote yet.
They are called FloProTek (http://www.easternlabsvc.com/floprotek.php) - Mercedes Medical Supply sent me the trial roll.
Thanks!
Matthew Roark- HT/HTL(ASCP)CM
Histology Specialist
Saint Francis Medical Center
211 Saint Francis Drive
Cape Girardeau, MO 63703
573-331-5267
mroark <@t> sfmc.net
http://www.sfmc.net
_______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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Message: 7
Date: Mon, 25 Feb 2013 14:57:25 -0500
From: "Susan Foreman" <sforeman <@t> labpath.com>
Subject: [Histonet] IgG4
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <002e01ce1392$554fa900$ffeefb00$@com>
Content-Type: text/plain; charset="us-ascii"
Has anyone worked up IgG4 on the Ventana Benchmark Ultra? What vendor?
How about running it on fresh frozen sections? FITC? Vendor?
Many Thanks for your input,
Susan
------------------------------
Message: 8
Date: Mon, 25 Feb 2013 20:09:08 +0000 (UTC)
From: nmhisto <@t> comcast.net
Subject: [Histonet] Streaking when Cutting
To: HISTONET <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<1822368949.1502216.1361822948867.JavaMail.root <@t> sz0075a.emeryville.ca.mail.comcast.net>
Content-Type: text/plain; charset=utf-8
I've been quietly observing from the sidelines, but I just can't pass this one up.?? One should never streak when cutting - it's distracting to fellow techs.?? Okay - back to my nap.
------------------------------
Message: 9
Date: Mon, 25 Feb 2013 20:27:55 +0000
From: "Scott, Allison D" <Allison.Scott <@t> harrishealth.org>
Subject: [Histonet] Waste Alcohol
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<FA607DC3D1ED7C46A9546820A3EB877F0A1E197F <@t> LBMSG02.hchd.local>
Content-Type: text/plain; charset="us-ascii"
Hello to all in histoland. Is there anyone putting alcohol into a waste drum like for xylene. We have a company that takes away our xylene. Now the safety people are suggesting that we treat the alcohol the same way. We have always poured the alcohol down the drain. It seems very expensive. Your help in this matter will be greatly appreciated.
Allison Scott HT(ASCP)
Supervisor, Histology Lab
LBJ Hospital
Harris Health System
Office: 713-566-5287
Lab: 713-566-5287
CONFIDENTIALITY NOTICE:
If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system.
To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited.
------------------------------
Message: 10
Date: Mon, 25 Feb 2013 20:33:27 +0000
From: "Rathborne, Toni" <trathborne <@t> somerset-healthcare.com>
Subject: [Histonet] RE: Waste Alcohol
To: "'Scott, Allison D'" <Allison.Scott <@t> harrishealth.org>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<3AD061FE740D464FAC7BF6B5CFB75707599DF5C3 <@t> smcmail02.somerset-healthcare.com>
Content-Type: text/plain; charset="us-ascii"
We have been disposing of our alcohols like this for years. This would include alcoholic eosin too. We are allowed to mix the waste xylene with the alcohols because they are the same classification, and treated the same way.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Scott, Allison D
Sent: Monday, February 25, 2013 3:28 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Waste Alcohol
Hello to all in histoland. Is there anyone putting alcohol into a waste drum like for xylene. We have a company that takes away our xylene. Now the safety people are suggesting that we treat the alcohol the same way. We have always poured the alcohol down the drain. It seems very expensive. Your help in this matter will be greatly appreciated.
Allison Scott HT(ASCP)
Supervisor, Histology Lab
LBJ Hospital
Harris Health System
Office: 713-566-5287
Lab: 713-566-5287
CONFIDENTIALITY NOTICE:
If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system.
To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited.
_______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 11
Date: Mon, 25 Feb 2013 15:02:10 -0600
From: "Horn, Hazel V" <HornHV <@t> archildrens.org>
Subject: [Histonet] RE: Waste Alcohol
To: "'Scott, Allison D'" <Allison.Scott <@t> harrishealth.org>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<25A4DE08332B19499904459F00AAACB719BE135634 <@t> EVS1.archildrens.org>
Content-Type: text/plain; charset="us-ascii"
Our xylene and alcohol go into a waste drum to be disposed of. We do recycle both but of course we still have waste to dispose of.
Hazel Horn
Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hornhv <@t> archildrens.org
archildrens.org
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Scott, Allison D
Sent: Monday, February 25, 2013 2:28 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Waste Alcohol
Hello to all in histoland. Is there anyone putting alcohol into a waste drum like for xylene. We have a company that takes away our xylene. Now the safety people are suggesting that we treat the alcohol the same way. We have always poured the alcohol down the drain. It seems very expensive. Your help in this matter will be greatly appreciated.
Allison Scott HT(ASCP)
Supervisor, Histology Lab
LBJ Hospital
Harris Health System
Office: 713-566-5287
Lab: 713-566-5287
CONFIDENTIALITY NOTICE:
If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system.
To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited.
_______________________________________________
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Thank you.
------------------------------
Message: 12
Date: Mon, 25 Feb 2013 21:18:12 +0000
From: Mike Pence <mpence <@t> grhs.net>
Subject: [Histonet] RE: Waste Alcohol
To: "Scott, Allison D" <Allison.Scott <@t> harrishealth.org>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <D6DF96C438FB8D42B04B2345A9452CF5AEDB <@t> MAIL-MB01.grhs.net>
Content-Type: text/plain; charset="us-ascii"
You should be recycling both xylene and alcohol. Cost savings compared to disposal cost will pay for a recycler in 3-5 years.
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] on behalf of Scott, Allison D [Allison.Scott <@t> harrishealth.org]
Sent: Monday, February 25, 2013 2:27 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Waste Alcohol
Hello to all in histoland. Is there anyone putting alcohol into a waste drum like for xylene. We have a company that takes away our xylene. Now the safety people are suggesting that we treat the alcohol the same way. We have always poured the alcohol down the drain. It seems very expensive. Your help in this matter will be greatly appreciated.
Allison Scott HT(ASCP)
Supervisor, Histology Lab
LBJ Hospital
Harris Health System
Office: 713-566-5287
Lab: 713-566-5287
CONFIDENTIALITY NOTICE:
If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system.
To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited.
_______________________________________________
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Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 13
Date: Mon, 25 Feb 2013 13:53:22 -0800 (PST)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Waste Alcohol
To: "Scott, Allison D" <Allison.Scott <@t> harrishealth.org>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<1361829202.27805.YahooMailNeo <@t> web163104.mail.bf1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
I always dumped the ethanol down the drain. I never recycled it because it takes almost 3 times more time that recycling xylene (3 times the cost) and the recycled alcohol is seldom of good quality.
When I stopped using xylene I had no more use (and exposure) when recycling.
Ren? J.
From: "Scott, Allison D" <Allison.Scott <@t> harrishealth.org>
To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
Sent: Monday, February 25, 2013 3:27 PM
Subject: [Histonet] Waste Alcohol
Hello to all in histoland.? Is there anyone putting alcohol into a waste drum like for xylene.? We have a company that takes away our xylene.? Now the safety people are suggesting that we treat the alcohol the same way.? We have always poured the alcohol down the drain.? It seems very expensive.? Your help in this matter will be greatly appreciated.
Allison Scott HT(ASCP)
Supervisor, Histology Lab
LBJ Hospital
Harris Health System
Office: 713-566-5287
Lab: 713-566-5287
CONFIDENTIALITY NOTICE:
If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system.
To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged.? This e-mail may also be confidential and/or privileged under Texas law.? The e-mail is for the use of only the individual or entity named above.? If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 14
Date: Mon, 25 Feb 2013 13:47:34 -0800
From: "Karla J. C." <karla <@t> dermatopathologynorthwest.com>
Subject: [Histonet] Dako autostainer service
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<000f01ce13a1$b8de93a0$2a9bbae0$@dermatopathologynorthwest.com>
Content-Type: text/plain; charset="us-ascii"
Hi All,
Help.Dako just recently informed us that they would no longer provide a Service contract for our immuno autostainer. Is anyone else experiencing this? Does anyone know of any other service people that may be able to work on this equipment (model 3400 year 2003)? We are located in Seattle.
Thanks,
Karla Carlmas
Dermatopathology Northwest
------------------------------
Message: 15
Date: Tue, 26 Feb 2013 01:55:58 -0600
From: <Susan.Walzer <@t> HCAHealthcare.com>
Subject: [Histonet] RE: CAP checklist question
To: <Valerie.Hannen <@t> parrishmed.com>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<4BF03F5404EBDE409AF9232DA74B9DED2DFF10BF41 <@t> FWDCWPMSGCMS09.hca.corpad.net>
Content-Type: text/plain; charset="us-ascii"
We put in a procedure stating that should we change the schedule we will test a variety of tissue (10 different types) and have the pathologist assess the run. We have not changed a schedule in years but we seek to comply...
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie
Sent: Monday, February 25, 2013 1:21 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] CAP checklist question
Hi gang..
I am working on a self-inspection CAP checklist and came across the following:
ANP.23120 Tissue Processor
Tissue processing schedules are validated. Note: New tissue processing schedules must be validated against the standard laboratory processing schedule.
Evidence of compliance: Written procedure for validation of new tissue processing schedules AND
WC records documenting validation
What do you all make of this?? We have not had any new processing schedules introduced into our lab in years. So, I really don't know how to proceed on this one.
Any insite will be greatly appreciated.
Valerie A. Hannen, MLT(ASCP),HTL,SU(FL)
Histology Section Chief
Parrish Medical Center
951 N. Washington Ave.
Titusville, Florida 32976
Phone:(321) 268-6333 ext. 7506
Fax: (321) 268-6149
valerie.hannen <@t> parrishmed.com
=============
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Message: 16
Date: Tue, 26 Feb 2013 09:14:28 -0500
From: "Amy Porter" <portera <@t> msu.edu>
Subject: [Histonet] Question for Alkaline Phosphatase / Biotin System
Users
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <000d01ce142b$96d73ac0$c485b040$@edu>
Content-Type: text/plain; charset="us-ascii"
We are using standard avidin / biotin staining with alkaline phosphatase labeling and sometimes, not every time and not every slide in the same run we get residual blotchy spots and streaking of the reaction on and off the tissue. We have increased to two rinses after primary and two rinses after alk phos reagent prior to substrate; still happening on some slides...anyone else have this experience or type of problem? Any experiences or suggestions are appreciated. Just can't figure out what is causing it..
Amy S. Porter, HT(ASCP) QIHC
Michigan State University
Investigative HistoPathology Laboratory
portera <@t> msu.edu
------------------------------
Message: 17
Date: Tue, 26 Feb 2013 09:27:03 -0600
From: "Horn, Hazel V" <HornHV <@t> archildrens.org>
Subject: RE: [Histonet] Waste Alcohol
To: "'Rene J Buesa'" <rjbuesa <@t> yahoo.com>, "Scott, Allison D"
<Allison.Scott <@t> harrishealth.org>, "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<25A4DE08332B19499904459F00AAACB719BE13563A <@t> EVS1.archildrens.org>
Content-Type: text/plain; charset="iso-8859-1"
The ability to put alcohol down the drain is regulated by your wastewater utility.
Hazel Horn
Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hornhv <@t> archildrens.org
archildrens.org
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Monday, February 25, 2013 3:53 PM
To: Scott, Allison D; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Waste Alcohol
I always dumped the ethanol down the drain. I never recycled it because it takes almost 3 times more time that recycling xylene (3 times the cost) and the recycled alcohol is seldom of good quality.
When I stopped using xylene I had no more use (and exposure) when recycling.
Ren? J.
From: "Scott, Allison D" <Allison.Scott <@t> harrishealth.org>
To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
Sent: Monday, February 25, 2013 3:27 PM
Subject: [Histonet] Waste Alcohol
Hello to all in histoland.? Is there anyone putting alcohol into a waste drum like for xylene.? We have a company that takes away our xylene.? Now the safety people are suggesting that we treat the alcohol the same way.? We have always poured the alcohol down the drain.? It seems very expensive.? Your help in this matter will be greatly appreciated.
Allison Scott HT(ASCP)
Supervisor, Histology Lab
LBJ Hospital
Harris Health System
Office: 713-566-5287
Lab: 713-566-5287
CONFIDENTIALITY NOTICE:
If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system.
To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged.? This e-mail may also be confidential and/or privileged under Texas law.? The e-mail is for the use of only the individual or entity named above.? If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited.
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Thank you.
------------------------------
Message: 18
Date: Tue, 26 Feb 2013 10:55:14 -0500
From: "Podawiltz, Thomas" <tpodawiltz <@t> lrgh.org>
Subject: RE: [Histonet] Waste Alcohol
To: "Horn, Hazel V" <HornHV <@t> archildrens.org>, 'Rene J Buesa'
<rjbuesa <@t> yahoo.com>, "Scott, Allison D"
<Allison.Scott <@t> harrishealth.org>, "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<38667E7FB77ECD4E91BFAEB8D986386324FCF3C6D9 <@t> LRGHEXVS1.practice.lrgh.org>
Content-Type: text/plain; charset="iso-8859-1"
Reagent Alcohol contains methanol, which is a U-listed EPA hazardous chemical and cannot be poured down the drain.
Tom Podawiltz HT (ASCP)
Histology Section Head/Laboratory Safety Officer.
LRGHealthcare
Laconia, NH 03246
603-524-3211 ext: 3220
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V
Sent: Tuesday, February 26, 2013 10:27 AM
To: 'Rene J Buesa'; Scott, Allison D; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Waste Alcohol
The ability to put alcohol down the drain is regulated by your wastewater utility.
Hazel Horn
Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hornhv <@t> archildrens.org
archildrens.org
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Monday, February 25, 2013 3:53 PM
To: Scott, Allison D; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Waste Alcohol
I always dumped the ethanol down the drain. I never recycled it because it takes almost 3 times more time that recycling xylene (3 times the cost) and the recycled alcohol is seldom of good quality.
When I stopped using xylene I had no more use (and exposure) when recycling.
Ren? J.
From: "Scott, Allison D" <Allison.Scott <@t> harrishealth.org>
To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
Sent: Monday, February 25, 2013 3:27 PM
Subject: [Histonet] Waste Alcohol
Hello to all in histoland.? Is there anyone putting alcohol into a waste drum like for xylene.? We have a company that takes away our xylene.? Now the safety people are suggesting that we treat the alcohol the same way.? We have always poured the alcohol down the drain.? It seems very expensive.? Your help in this matter will be greatly appreciated.
Allison Scott HT(ASCP)
Supervisor, Histology Lab
LBJ Hospital
Harris Health System
Office: 713-566-5287
Lab: 713-566-5287
CONFIDENTIALITY NOTICE:
If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system.
To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged.? This e-mail may also be confidential and/or privileged under Texas law.? The e-mail is for the use of only the individual or entity named above.? If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited.
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The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer.
Thank you.
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THIS MESSAGE IS CONFIDENTIAL.
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------------------------------
Message: 19
Date: Tue, 26 Feb 2013 09:56:12 -0600
From: "Brendal Finlay" <brendal.finlay <@t> medicalcenterclinic.com>
Subject: Re: [Histonet] Waste Alcohol
To: "Rene J Buesa" <rjbuesa <@t> yahoo.com>, "Scott, Allison D"
<Allison.Scott <@t> harrishealth.org>, histonet <@t> lists.utsouthwestern.edu
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <4fcdbb6eed8fb2fc1e5ea8e615ce00ee <@t> medicalcenterclinic.com>
Content-Type: text/plain; charset="utf-8"
You??will probably want to contact your local or state office of environmental protection to find out what is required in your area.??
??
The EPA lists hazardous wastes in 40 CFR 261.30.?? Even if your waste is not on this list, it may have hazardous characteristics such as:
ignitability, corrosivity, reactivity, and toxicity characteristic.??
Ignitability pertains to flashpoints less than 140 F and/or aqueous solutions with alcohol content greater than or equal to 24 percent.??
??
Florida's DEP has different regulations dependent upon the quantity of hazardous waste generated.?? Some localities may require approval to dispose of alcohol down the drain.?? If you don't have this approval, you have to dispose of the waste according to??your state??and??federalregulations.??
??
To reduce the quantity of hazardous waste we generate, we began to recycle using CBG Biotech's distillation unit.?? We have had great results with it.?? While alcohol does take longer to recycle than xylene, our supply costs have been greatly reduced and we have worked this into our weekly schedule very well.
??
Some things to consider when using recycled alcohol:
Test each finished alcohol run with a hydrometer to know what percentage it is.??
Do not use recycled alcohol at the end of the stain line after eosin (it will wash the eosin out).
Do not use recycled alcohol as the last alcohol before xylene on the processor.
Finally, do not recycle the first alcohols on the stain line after xylene (at least on our recycler). This is disposed of in our flammable waste containers to be picked up.
??
Here???s a link to the EPA???s managing hazardous waste pdf - http://www.epa.gov/osw/hazard/generation/sqg/handbook/k01005.pdf
Brendal Finlay, HT (ASCP)
http://medicalcenterclinic.com
-----Original message-----
From: Rene J Buesa rjbuesa <@t> yahoo.com
Date: Mon, 25 Feb 201316:53:22 -0600
To: "Scott, Allison D" Allison.Scott <@t> harrishealth.org, "histonet <@t> lists.utsouthwestern.edu" histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Waste Alcohol
I always dumped the ethanol down the drain. I never recycled it because it takes almost 3 times more time that recycling xylene (3 times the cost) and the recycled alcohol is seldom of good quality.
When I stopped using xylene I had no more use (and exposure) when recycling.
Ren?? J.
From: "Scott, Allison D"
To: "histonet <@t> lists.utsouthwestern.edu"
Sent: Monday, February 25, 2013 3:27 PM
Subject: [Histonet] Waste Alcohol
Hello to all in histoland.?? Is there anyone putting alcohol into a waste drum like for xylene.?? We have a company that takes away our xylene.?? Now the safety people are suggesting that we treat the alcohol thesame way.?? We have always poured the alcohol down the drain.?? It seems very expensive.?? Your help in this matter will be greatly appreciated.
Allison Scott HT(ASCP)
Supervisor, Histology Lab
LBJ Hospital
Harris Health System
Office: 713-566-5287
Lab: 713-566-5287
CONFIDENTIALITY NOTICE:
If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system.
To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged.?? This e-mail may also be confidential and/or privileged under Texas law.?? The e-mail is forthe use of only the individual or entity named above.?? If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is
strictly prohibited.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
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------------------------------
Message: 20
Date: Tue, 26 Feb 2013 09:33:37 -0800 (PST)
From: Sheila Fonner <fourfonners <@t> yahoo.com>
Subject: [Histonet] streaks
To: "cthornton <@t> dahlchase.com" <cthornton <@t> dahlchase.com>, Histonet
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<1361900017.78056.YahooMailNeo <@t> web140605.mail.bf1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Hi Claire,
?
I may be grasping at straws here, but I went to an NSH workshop once where this very question was posed.? You won't believe what the cause was.? They had a bad lot of blades.? The whole lot was nicked, so changing to a new knife or a new box of knives did not help the problem.? Changing the lot did the trick.? I don't know if this is your problem or not, but I thought it was very interesting.
?
Sheila HT (ASCP)
Knoxville, TN
------------------------------
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End of Histonet Digest, Vol 111, Issue 37
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