[Histonet] RE: Type I Water

[Morken, Timothy]

Nancy, You don't need Type 1 water for waterbaths. Type 1 (CLSI Reagent Grade) is for chemistry analytics - for which you want extremely pure water. For water-baths filtered tap water may be fine if it is filtered. For waterbaths you are mainly concerned about dirt, bacterial and sometimes mineral contaminants. Distilled water is probably the best since it is cleaner than tap water. 

We use type 1 for histochemistry stain applications but don't use very much so we buy it by the gallon from Thermo Fisher Scientific (cat# 9800-1). They call it NERL Reagent Grade Water. (NERL = NERL Diagnostic's, a Thermo Fisher company that makes medical-grade reagents. NERL used to mean New England Reagent Laboratory).


Tim Morken
UCSF Pathology


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt
Sent: Friday, February 15, 2013 6:26 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Type I Water

Clearly I sent this too quickly (early) - this is in regards to the water in our tissue float baths - does it HAVE to be Type I?
Thanks
Nancy

Good Morning-
I feel like I am racking up frequent flier miles on Histonet lately:) We are seeing bacteria in our type I.  This requires us to obtain our Type I from another of our lab sites.  Do I have any other options?  Like tap water?
Nancy Schmitt HT, MLT(ASCP)




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