[Histonet] standard protocol

[Bea DeBrosse-Serra]

I sure have never heard of a standard protocol like this with any pharmaceutical company I have ever worked for. This is disturbing!

Beatrice DeBrosse-Serra HT(ASCP)QIHC
Isis Pharmaceuticals
Antisense Drug Discovery
2855 Gazelle Ct.
Carlsbad, CA 92010
760-603-2371



-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Tony Henwood (SCHN)
Sent: Thursday, February 14, 2013 3:31 PM
To: 'Histopatty'; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] standard protocol

Hi Kay,

Who was the idiot who sent this to you?

Why bother cutting the sections if they will float off in the xylene, you might as well just send them blank slides.

The companies are willing to spend millions of dollars on the development but couldn't be bothered seeking professional advice before they send out mandatory protocols like this.

Unfortunately I regularly see this and usually send back a letter stating that to follow their protocol we will be charging more for the stupidity. 
I do not tell them what they should be doing unless they are willing to pay for my advice.

You can forward my reply on to them if you so wish.

Here ends my Friday rant!!

Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Histopatty
Sent: Friday, 15 February 2013 3:02 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] standard protocol


We are getting instructions from a pharmaceutical company who needs slides, with instructions stating "sections should be floated per standard protocol, xylene dipped from 3 minutes and dried for 10 minutes."  I'm reaching out to those of you who routinly deal with research protocols and wondering is the "standard protocol" the normal float, dri, melt?

Patricia Eneff
for
Kay Pierce

Please respond to sharon.pierce <@t> hcahealthcare.com if possilbe.  
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