[Histonet] H&E Destain protocol

[HERRINGTON, SHEILA]

Not if it is just from processing. Eosin from processing will not affect any IHC or Special stains. If it was H&E stained you just need to take back through Xylene, alcohol to water.  The alcohol will remove some of the eosin, and the IHC process will not be affected by the stains. It will simply replace them. Usually I do the same process as well with a control just to quality control it.  


Sheila Herrington, IHC Kelowna General
Grade 3, Immunohistochemistry, Histolopathogy
Kelowna General Hospital



-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Maribel Santiago
Sent: Thursday, February 14, 2013 11:52 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] H&E Destain protocol


Hello Histonet,
I have samples that were stained during fixation with Eosin. First question, since I  will do IHC on them, do I need to get rid of the eosin like you do with an already H&E stained slide? If so, that brings me to my second question, does anyone have a protocol that are willing to share with me along with helpful hints?? 
Thanks,Minnie
 
 
 
 
 
 
 
 
 

 
 
 
 
 
 



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