[Histonet] Stain to ID eosinophils specifically

[Amos Brooks]

Hi Colleen,
     Although I was not able to locate the specific reference you listed,
the two stains that come to mind are Lendrum's Carbol Chromotrope and Congo
Red. While they are not *really* specific for eosinophils, they do stain
eosinophils well. It is fairly easy to differentiate an eosinophil from
amyloid in Congo Red. I am including a link to Eastwood & Cole's Congo Red
because the procedure specifically mentions eosinophils, but I have used
Highman's (the more common version) to identify them. Of the two the Congo
Red techniques seem to work better which is great since they are a bit
easier (& less messy) than the carbol chromotrope.

     Here are a couple of references for both:
http://stainsfile.info/StainsFile/stain/cell/lendrum-carbolchromotrope.htm
and
http://stainsfile.info/StainsFile/stain/amyloid/congoeastwood.htm

Good Luck,
Amos



On Tue, Feb 12, 2013 at 1:00 PM,
<histonet-request <@t> lists.utsouthwestern.edu>wrote:

> Message: 6
> Date: Mon, 11 Feb 2013 16:36:29 -0600
> From: Colleen Forster <cforster <@t> umn.edu>
> Subject: [Histonet] Stain to ID eosinophils specifically
> To: Histonet <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <5119726D.7000509 <@t> umn.edu>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Does anyone have access to this reference for me? I have a person looking
> to ID eosinophils in fat samples.
> I am looking for a stain that will make finding them easier....this
> reference was mentioned....any other suggestions? These are FFPE samples.
>
> Suggested Ref ex. LIllie 4th Ed. pp750.  ref.Am.J.Clin.Path.55:283,1971.)
>
> Thanks in advance...
>
> Colleen L. Forster
>