[Histonet] Her2neu controls

[Mark Tarango]

Hi Jim,

We run the control slide that Ventana sends (although not the best control)
but we add a section from our tissue control block before staining.  The
tissue control block contains a 0+, weakly staining 1+, and a 3+.  We use
the ventana/tissue control slide as our batch control.  This slide must be
QC'd by a pathologist before any HER2 IHC is sent to pathologists for
scoring.  In addition, each patient slide has a section of the tissue
control.  We use a weakly staining 1+ in the tissue control to monitor any
slight change in the staining.  If the 1+ suddenly is a 0+, then we have a
problem.  If you were just running a 3+ control, you probably wouldn't
notice the staining difference in the control.

Mark T.

On Mon, Feb 11, 2013 at 2:53 PM, Vickroy, Jim <Vickroy.Jim <@t> mhsil.com> wrote:

> When we order the antibody from Ventana they include some control slides
> with tissues that are 0, 1+, 2+, and 3+.   Currently the lab where we have
> sent our Her2neu's to be done only uses a 3+ control.  I realize that a
> control that had several levels of staining would be ideal but I am trying
> to find out how other diagnostic labs are handling their controls for
> Her2neu.
> Thanks
>
>
>
>
> James Vickroy BS, HT(ASCP)
>
> Surgical  and Autopsy Pathology Technical Supervisor
> Memorial Medical Center
> 217-788-4046
>
>
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