[Histonet] Could these samples have been saved?

[Maxim Peshkov]

Kevin!
We have had a similar problem, when our
tissues totally dried on air during
more than 18 hours after acetone and
looked as a stones.
We put our bloks into Luna's solution
for repocessing tissue with formaldehyde,
sodium acetate and glycerine
(Laboratory methods in histotechnology,
AFIP, 1992) and did as described in this chapter.
It works great. We successfully sectioned
all the blocks and have had accetable
slides for diagnosis.

Some years after we started to use
mineral oil and isopropanol for
processing. Isopropanol will not
such severe drying tissues as acetone
and ethanol.

Maxim Peshkov,
Russia,
Taganrog.

Original message---
From: "Johnson, Kevin" <KJohnson <@t> med.miami.edu>
To: "'histonet <@t> lists.utsouthwestern.edu'" <histonet <@t> lists.utsouthwestern.edu> 
Sent: Friday, February 8, 2013 1:00 PM
Subject: [Histonet] Could these samples have been saved?

Dear all,

During an overnight tissue processing cycle,
a malfunction occurred such that the sample
basket was suspended in mid-air for several
hours at probably the worst spot in which to
do so---after the final absolute ethanol
of the dehydration series.
I continued the process manually in the
morning, and carried it through blocking
and attempted sectioning.
However, the samples (mouse skin and fat)
had been converted to uncuttable rocks.

In hindsight, should I have attempted to
rehydrate and reprocess these samples in
an attempt to glean even minimal information
from them?  Or is there no way to unmummify
a mummy?

Regards,

Kevin Johnson
University of Miami
Diabetes Research Institute
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

                          mailto:Maxim_71 <@t> mail.ru