[Histonet] Could these samples have been saved?

[joelle weaver]

It was my understanding that with MW-assited processing, that they could not be put back through the MW assisted fixation part of the program. That is not to say that you couldn't rehydrate tissues and then evaluate and pass them through the rest if they were fixed, such as the alcohols, isopropanol and/or paraffin. I supposed you could conventionally fix if that were the issue, but I do remember when using these instruments that I didn't  "double microwave". Instead,  I just made an edited program to take it to wax depending on the situation. Most of the software made this pretty easy to do. Does anyone know differently? 




Joelle Weaver MAOM, HTL (ASCP) QIHC
 > Date: Fri, 8 Feb 2013 13:22:15 -0500
> From: kgrobert <@t> rci.rutgers.edu
> To: KJohnson <@t> med.miami.edu
> Subject: Re: [Histonet] Could these samples have been saved?
> CC: histonet <@t> lists.utsouthwestern.edu
> 
> I don't know about your samples now, but for future reference, you might
> find this interesting:
> http://realhistoryww.com/world_history/ancient/Misc/Data/Study_mummified_soft_tissues.htm
> 
> Kathleen Roberts
> 
> Principal Lab Technician
> Neurotoxicology Labs
> Molecular Pathology Facility Core
> Dept of Pharmacology & Toxicology
> Rutgers, the State University of NJ
> 41 B Gordon Road
> Piscataway, NJ 08854
> (848) 445-1443
> FAX (732) 445-6905
> 
> > Dear all,
> >
> > During an overnight tissue processing cycle, a malfunction occurred such
> > that the sample basket was suspended in mid-air for several hours at
> > probably the worst spot in which to do so---after the final absolute
> > ethanol of the dehydration series. I continued the process manually in the
> > morning, and carried it through blocking and attempted sectioning.
> > However, the samples (mouse skin and fat) had been converted to uncuttable
> > rocks.
> >
> > In hindsight, should I have attempted to rehydrate and reprocess these
> > samples in an attempt to glean even minimal information from them?  Or is
> > there no way to unmummify a mummy?
> >
> > Regards,
> >
> > Kevin Johnson
> > University of Miami
> > Diabetes Research Institute
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> >
> 
> 
> 
> 
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