[Histonet] Processor dehydration cycles..

[Rene J Buesa]

I usually started with 80%EthOL but it does not "harm" starting with 70%EthOL and it is even better, from the theoretically view point. So if you have the "space" in your protocol, keep the 70%EthOL
René J.

From: Tom McNemar <TMcNemar <@t> lmhealth.org>
To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu> 
Sent: Thursday, February 7, 2013 10:14 AM
Subject: [Histonet] Processor dehydration cycles..

Hello all,

I was wondering what most people use as the first reagent after the formalins on their tissue processor?  We have always used a sequence of 70%, 80%, 95%, and 100% but is anyone using 80% or even 95% to start their dehydration?

Thanks in advance.

Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcnemar <@t> lmhealth.org<mailto:tmcnemar <@t> lmhealth.org>
www.LMHealth.org<file:///C:\Documents%20and%20Settings\TMCNEMAR\Application%20Data\Microsoft\Signatures\www.LMHealth.org>

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