FW: [Histonet] p16

[Cynthia Pyse]

Yes we monitor the temp and humidity. Retrieval is made fresh for every run.
We run the PT for the p16 in a water bath since we only run a few slides at
a time, but the water bath temp in also monitored. I should say that there
is some staining in these slides just not what the control usually looks
like. If we repeat the slides they do stain correctly. There is no
difference in the slide prep or the staining procedure. Any ideas.
Cindy 

Cindy Pyse, CLT, HT (ASCP)
Laboratory Manager
X-Cell Laboratories
20 Northpointe Parkway Suite 100
Amherst, NY 14228
716-250-9235 etx. 232
e-mail cpyse <@t> x-celllab.com

-----Original Message-----
From: Ross Benik [mailto:ross <@t> premierlab.com] 
Sent: Wednesday, February 06, 2013 1:26 PM
To: Cynthia Pyse
Subject: RE: [Histonet] p16

Do you monitor the temperature and humidity inside the Link48? How often do
you prepare your retrieval solution/are you using the PT mods?

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse
Sent: Wednesday, February 06, 2013 11:11 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] p16

Happy Wednesday Everyone

Has anyone been having consistency problems with their p16 staining since
MTM has been taken over by Ventana?



One day the procedure works another day it doesn't. Same procedure, no
variation in the temperature on pretreatment, run on the Dako 48, with no
errors. Every other IHC works consistently but the p16, anyone have any
thoughts? I do make the DAB fresh daily as suggested in the instructions, it
is the same kit that has been running. The only thing I can think of is we
do sometimes run the p16 twice a day without making fresh DAB, but the
instructions say it can be used within a 24 hour period.



This problem is driving me crazy! It just doesn't make any sense. Any help
would be appreciated. Thanks in advance.

Cindy



Cindy Pyse, CLT, HT (ASCP)

Laboratory Manager

X-Cell Laboratories

20 Northpointe Parkway Suite 100

Amherst, NY 14228

716-250-9235 etx. 232

e-mail cpyse <@t> x-celllab.com



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