[Histonet] p16

[Cynthia Pyse]

Happy Wednesday Everyone

Has anyone been having consistency problems with their p16 staining since
MTM has been taken over by Ventana? 

 

One day the procedure works another day it doesn't. Same procedure, no
variation in the temperature on pretreatment, run on the Dako 48, with no
errors. Every other IHC works consistently but the p16, anyone have any
thoughts? I do make the DAB fresh daily as suggested in the instructions, it
is the same kit that has been running. The only thing I can think of is we
do sometimes run the p16 twice a day without making fresh DAB, but the
instructions say it can be used within a 24 hour period. 

 

This problem is driving me crazy! It just doesn't make any sense. Any help
would be appreciated. Thanks in advance.

Cindy

 

Cindy Pyse, CLT, HT (ASCP)

Laboratory Manager

X-Cell Laboratories

20 Northpointe Parkway Suite 100

Amherst, NY 14228

716-250-9235 etx. 232

e-mail cpyse <@t> x-celllab.com