[Histonet] Xylene Substitutes

[Rene J Buesa]

The best solution to eliminate xylene is to use isopropyl alcohol mixed with mineral oil.
Xylene can be eliminated from staining by dewaxing with 2% aq. sol. of dishwasher soap.
Before coverslipping oven dry the stained sections and cover directly.
To clean tissue processors use a 2% mixture of strong lab lab-ware detergent.
Do all of the above and your lab will be xylene free.
René J.

From: Tom McNemar <TMcNemar <@t> lmhealth.org>
To: 'Adrienne Anderson' <rennie1108 <@t> yahoo.com>; "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu> 
Sent: Wednesday, February 6, 2013 5:36 AM
Subject: RE: [Histonet] Xylene Substitutes

Not familiar with SubX but we have used Americlear for many years with good results.  You just can't beat Xylene for some things though and still keep a little around.

Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcnemar <@t> lmhealth.org
http://www.lmhealth.org/


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Adrienne Anderson
Sent: Tuesday, February 05, 2013 4:58 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Xylene Substitutes

Hello all,

My lab is looking into xylene substitutes, and I'd love some feedback on what other labs are using. We currently use SubX, but are there other items out there more economical?

Thanks,
Adrienne
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