[Histonet] Staining for Phospholipids

[Duraine, Lita]

Hello Histoland,

Someone needs help in our lab with some fluorescent staining.  He is staining  drosophila retina with antibody PI4.5P2, or PIP2 for looking at Phospholipids around the membranes.  He is using 4% Paraformaldehyde and Triton X in his protocol and is getting  inconsistent results and the signals are weak even at the nucleus.  Does anyone have a protocol or recipe that might give better results?

Thank you,

Lita Duraine
EM Technologist
Bellen Lab
HHMI- Molecular Genetics
Duncan Neurological Research Institute
1250 Moursund St.
Houston, TX 77030
Rm: N1165.17
MS: N1125.50
832-824-8772 TEM Room
979-549-6526 Cell
http://flypush.imgen.bcm.tmc.edu/lab/people/lita.php