[Histonet] 4% Paraformaldehyde

[Morken, Timothy]

Rhonda, Your question is a little to general.

4% formalin made from paraformaldehyde is similar to 10% formaldehyde. So, the question is, for what are you storing the tissue? 

If it is for morphology studies, then it can be weeks or months.

If it is for immunochemistry then days to weeks is generally ok but depends on the antigen. Usually people will fix for 24 hours max and then embed. But most antigens survive much longer exposure to formalin.

More details will bring more precise answers!


Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Garlick, Rhonda
Sent: Friday, February 01, 2013 10:18 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] 4% Paraformaldehyde


I got one response from my question below. Does anyone else have hard and fast rules with regards to the length of time tissues should/can be stored in 4% Para?

Thanks, Rhonda




On 1/22/13 1:08 PM, "Garlick, Rhonda" <RHONDA.GARLICK <@t> UCDENVER.EDU> wrote:

>I've been doing some on-line searching with regards to the length of 
>time tissues can remain in 4% Paraformaldehyde, but can't seem to get 
>any definite answers. Can anyone help.
>
>Thanks, Rhonda
>Univ. of Denver
>Denver, CO
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>Histonet <@t> lists.utsouthwestern.edu
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