[Histonet] Expiration dates of ammonium sulphide

[Morken, Timothy]

Am sulfide is generally used in excess in procedures so the concentration is not critical. 

My approach is to buy the smallest amount possible and use it up before the expiration. Then there is no need to worry about activity, expirations, extending life, etc. 

For instance we use it for muscles and buy it in 25ml quantity which lasts about 3 months. We never have to worry about expiration with that kind of turnover.

That is harder with dry chemicals but fairly easy with liquids.


Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center



-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Friday, February 01, 2013 6:07 AM
To: Tony Henwood (SCHN); histonet
Subject: Re: [Histonet] Expiration dates of ammonium sulphide

Ammonium sulfide loses its activity with time. You will have to test it. Please review a recent thread on this subject in HistoNet.
René J.

From: Tony Henwood (SCHN) <tony.henwood <@t> health.nsw.gov.au>
To: histonet <histonet <@t> lists.utsouthwestern.edu>
Sent: Thursday, January 31, 2013 6:39 PM
Subject: [Histonet] Expiration dates of ammonium sulphide

Hi all,

I have a chemical question.

We use 20% ammonium sulphide and I am wondering whether it loses its efficiency over time. Is there an expiry date on this solution and is there a test one can do to determine the concentration of aged 20% solutions?

I have searched and come up with nuthin!

Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 


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