From collette2 <@t> llnl.gov Fri Feb 1 01:02:29 2013 From: collette2 <@t> llnl.gov (Collette, Nicole M.) Date: Fri Feb 1 01:02:34 2013 Subject: [Histonet] pancreas pH or proteases inhibit LacZ enzyme activity? In-Reply-To: <1539B7EADCA2AD42BAFA924EB57A5A1405B9AD@PRDEXMBX-03.the-lab.llnl.gov> Message-ID: <1539B7EADCA2AD42BAFA924EB57A5A1405B9C5@PRDEXMBX-03.the-lab.llnl.gov> Hello, Histonet experts, I have a puzzling bit of data that I am trying to resolve. Our group and another group have performed LacZ stain on mouse pancreas to identify cells expressing a LacZ reporter (the other group has published this expression pattern). I am having trouble with the primary antibody for the protein that LacZ is supposed to report for, so as a backup I tried an antibody for LacZ, which has worked very well for us in other tissues in the past, and has always matched the pattern we see with LacZ stains. With LacZ antibody, I get very robust stain in the islets, (as well as light stain in the surrounding tissue consistent with the LacZ stain pattern) and I see a qualitative dose response to diabetes induction, which makes me think the antibody stain is real. However, we and another group have not seen any LacZ activity in the islets, only in the surrounding tissue. Could the LacZ enzymatic activity be disrupted ex vivo by the pH of the islets themselves, or protease activity in the islets, so we can pick it up by immunostain but not LacZ enzymatic stain? Any other expert opinions as to what's going on? The insulin and glucagon antibody stains are working like champs, beautiful specific signal with very low background. I have been doing LacZ stains on various parts and pieces for the better part of 6 years, this is an unusual case where the confirmation controls didn't match the LacZ stain. And to clarify, I am doing the LacZ (and other) immunostains on pancreas sections that were NOT stained enzymatically for LacZ activity, but are the same genotype. I use Rene's recommended IM embedding protocol for paraffin. On the LacZ side, I stain whole-mount tissue prior to embedding and sectioning, with attention to fixation times and pH of paramount importance. Thanks in advance for your help! Sincerely, Nicole Collette, Ph.D. Lawrence Livermore National Lab collette2@llnl.gov From avogaro <@t> science.unitn.it Fri Feb 1 03:46:35 2013 From: avogaro <@t> science.unitn.it (Laura Avogaro) Date: Fri Feb 1 03:46:44 2013 Subject: [Histonet] Cardiac tissue freezing Message-ID: <3530.192.168.178.78.1359711995.squirrel@www.science.unitn.it> Hi everyone! I have to flash freeze human atrial tissue biopsies. Does anyone can suggest me a protocol or some useful tips for this procedure in order to preserve the tissue architecture without artifacts formation? If is possible I prefer to avoid the use of any fixative. Thanks in advance Laura Laura Avogaro Center for Biomedical Technologies (BIOTECH) University of Trento Via delle Regole, 101 38123 Mattarello (TN) ? Italy Tel: +39 0461 283425 From rjbuesa <@t> yahoo.com Fri Feb 1 08:07:12 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Feb 1 08:07:19 2013 Subject: [Histonet] Expiration dates of ammonium sulphide In-Reply-To: <6D6BD1DE8A5571489398B392A38A71579D22EDE0@xmdb04.nch.kids> References: <6D6BD1DE8A5571489398B392A38A71579D22EDE0@xmdb04.nch.kids> Message-ID: <1359727632.99037.YahooMailNeo@web163103.mail.bf1.yahoo.com> Ammonium sulfide loses its activity with time. You will have to test it. Please review a recent thread on this subject in HistoNet. Ren? J. From: Tony Henwood (SCHN) To: histonet Sent: Thursday, January 31, 2013 6:39 PM Subject: [Histonet] Expiration dates of ammonium sulphide Hi all, I have a chemical question. We use 20% ammonium sulphide and I am wondering whether it loses its efficiency over time. Is there an expiry date on this solution and is there a test one can do to determine the concentration of aged 20% solutions? I have searched and come up with nuthin! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Fri Feb 1 10:14:54 2013 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Fri Feb 1 10:15:17 2013 Subject: [Histonet] Expiration dates of ammonium sulphide In-Reply-To: <1359727632.99037.YahooMailNeo@web163103.mail.bf1.yahoo.com> References: <6D6BD1DE8A5571489398B392A38A71579D22EDE0@xmdb04.nch.kids> <1359727632.99037.YahooMailNeo@web163103.mail.bf1.yahoo.com> Message-ID: <761E2B5697F795489C8710BCC72141FF05398A@ex07.net.ucsf.edu> Am sulfide is generally used in excess in procedures so the concentration is not critical. My approach is to buy the smallest amount possible and use it up before the expiration. Then there is no need to worry about activity, expirations, extending life, etc. For instance we use it for muscles and buy it in 25ml quantity which lasts about 3 months. We never have to worry about expiration with that kind of turnover. That is harder with dry chemicals but fairly easy with liquids. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, February 01, 2013 6:07 AM To: Tony Henwood (SCHN); histonet Subject: Re: [Histonet] Expiration dates of ammonium sulphide Ammonium sulfide loses its activity with time. You will have to test it. Please review a recent thread on this subject in HistoNet. Ren? J. From: Tony Henwood (SCHN) To: histonet Sent: Thursday, January 31, 2013 6:39 PM Subject: [Histonet] Expiration dates of ammonium sulphide Hi all, I have a chemical question. We use 20% ammonium sulphide and I am wondering whether it loses its efficiency over time. Is there an expiry date on this solution and is there a test one can do to determine the concentration of aged 20% solutions? I have searched and come up with nuthin! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Elizabeth.Cameron <@t> jax.org Fri Feb 1 11:28:50 2013 From: Elizabeth.Cameron <@t> jax.org (Elizabeth Cameron) Date: Fri Feb 1 11:28:55 2013 Subject: [Histonet] Benchmarking Message-ID: Histonet Users, Scientific Services at The Jackson Laboratory is conducting benchmarking surveys in an effort to develop an understanding of the best current practices offered by peer institutions. The goal is to gather quality data that can be used to assist us and all survey participants with operational assessment and planning. Survey participants will receive compiled results which will be de-identified before distribution. The link is below and must be completed by February 28th. Thank you in advance for your participation! http://www.surveymonkey.com/s/HistologySurvey2013 Elizabeth M. Cameron, HT, QIHC (ASCP) Histology Supervisor The Jackson Laboratory Bar Harbor, Maine 207-288-6326 The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as possible. From gmarcella <@t> nj-urology.com Fri Feb 1 11:58:51 2013 From: gmarcella <@t> nj-urology.com (Gail Marcella) Date: Fri Feb 1 11:58:57 2013 Subject: [Histonet] Procedure manuals Message-ID: <53B7C169C959BB43BEF79FCB77418B640C562BC3CF@njue1> Hi - Does anyone know if everyone that works in the lab has to sign off on every procedure in the manual, every single year??? From RHONDA.GARLICK <@t> UCDENVER.EDU Fri Feb 1 12:17:30 2013 From: RHONDA.GARLICK <@t> UCDENVER.EDU (Garlick, Rhonda) Date: Fri Feb 1 12:17:34 2013 Subject: [Histonet] 4% Paraformaldehyde In-Reply-To: Message-ID: I got one response from my question below. Does anyone else have hard and fast rules with regards to the length of time tissues should/can be stored in 4% Para? Thanks, Rhonda On 1/22/13 1:08 PM, "Garlick, Rhonda" wrote: >I've been doing some on-line searching with regards to the length of time >tissues can remain in 4% Paraformaldehyde, but can't seem to get any >definite answers. Can anyone help. > >Thanks, Rhonda >Univ. of Denver >Denver, CO >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Fri Feb 1 12:29:45 2013 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Fri Feb 1 12:30:00 2013 Subject: [Histonet] 4% Paraformaldehyde In-Reply-To: References: Message-ID: <761E2B5697F795489C8710BCC72141FF0539FF@ex07.net.ucsf.edu> Rhonda, Your question is a little to general. 4% formalin made from paraformaldehyde is similar to 10% formaldehyde. So, the question is, for what are you storing the tissue? If it is for morphology studies, then it can be weeks or months. If it is for immunochemistry then days to weeks is generally ok but depends on the antigen. Usually people will fix for 24 hours max and then embed. But most antigens survive much longer exposure to formalin. More details will bring more precise answers! Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Garlick, Rhonda Sent: Friday, February 01, 2013 10:18 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] 4% Paraformaldehyde I got one response from my question below. Does anyone else have hard and fast rules with regards to the length of time tissues should/can be stored in 4% Para? Thanks, Rhonda On 1/22/13 1:08 PM, "Garlick, Rhonda" wrote: >I've been doing some on-line searching with regards to the length of >time tissues can remain in 4% Paraformaldehyde, but can't seem to get >any definite answers. Can anyone help. > >Thanks, Rhonda >Univ. of Denver >Denver, CO >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From patpxs <@t> gmail.com Fri Feb 1 12:50:42 2013 From: patpxs <@t> gmail.com (Paula Sicurello) Date: Fri Feb 1 12:50:45 2013 Subject: [Histonet] 4% Paraformaldehyde In-Reply-To: References: Message-ID: Rhonda, What Tim says is true, it all depends on what the tissue is going to be used for. Provide more detailed information to get a more detailed answer. Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 On Fri, Feb 1, 2013 at 1:17 PM, Garlick, Rhonda wrote: > > I got one response from my question below. Does anyone else have hard and > fast rules with regards to the length of time tissues should/can be stored > in 4% Para? > > Thanks, Rhonda > > > > > On 1/22/13 1:08 PM, "Garlick, Rhonda" wrote: > >>I've been doing some on-line searching with regards to the length of time >>tissues can remain in 4% Paraformaldehyde, but can't seem to get any >>definite answers. Can anyone help. >> >>Thanks, Rhonda >>Univ. of Denver >>Denver, CO >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BZIMMERM <@t> gru.edu Fri Feb 1 13:25:37 2013 From: BZIMMERM <@t> gru.edu (Zimmerman, Billie) Date: Fri Feb 1 13:26:57 2013 Subject: [Histonet] Georgia Society of Histotechnology Message-ID: <7B3DEB32E69C034EACB479059C5DE3FF753DE6@EX-MLB-03.ad.georgiahealth.edu> This is a friendly reminder to renew your free membership with the Georgia Society of Histotechnology. If you have any questions or concerns, please send me an email at ZBillie58@gmail.com Thanks, Billie Zimmerman MT(ASCP)QIHC Secretary of GSH Augusta State University and Georgia Health Sciences University have consolidated to become Georgia Regents University. Effective January 9, 2013, my email address has changed to BZIMMERM@gru.edu. Please update your address book to reflect this change. From ibernard <@t> uab.edu Fri Feb 1 14:01:36 2013 From: ibernard <@t> uab.edu (Ian R Bernard) Date: Fri Feb 1 14:01:41 2013 Subject: [Histonet] RE: Procedure manuals In-Reply-To: <53B7C169C959BB43BEF79FCB77418B640C562BC3CF@njue1> References: <53B7C169C959BB43BEF79FCB77418B640C562BC3CF@njue1> Message-ID: Pre CAP every two years or as needed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gail Marcella Sent: Friday, February 01, 2013 11:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Procedure manuals Hi - Does anyone know if everyone that works in the lab has to sign off on every procedure in the manual, every single year??? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Feb 1 14:46:37 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Feb 1 14:46:41 2013 Subject: [Histonet] Procedure manuals In-Reply-To: <53B7C169C959BB43BEF79FCB77418B640C562BC3CF@njue1> References: <53B7C169C959BB43BEF79FCB77418B640C562BC3CF@njue1> Message-ID: <1359751597.70773.YahooMailNeo@web163104.mail.bf1.yahoo.com> Yes, that is the only way you will be able to show everybody knows and will comply with the procedures. This is of paramount importance for the annual evaluations. If you change any procedure, that will be signed; if procedures remain the same there is no need. Ren? J. From: Gail Marcella To: "histonet@lists.utsouthwestern.edu" Sent: Friday, February 1, 2013 12:58 PM Subject: [Histonet] Procedure manuals Hi - Does anyone know if everyone that works in the lab has to sign off on every procedure in the manual, every single year??? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From duraine <@t> bcm.edu Fri Feb 1 17:15:46 2013 From: duraine <@t> bcm.edu (Duraine, Lita) Date: Fri Feb 1 17:15:55 2013 Subject: [Histonet] Staining for Phospholipids Message-ID: <9D2C4E815F57784DAE6914BA54C82324DF0392778F@EXCMSMBX06.ad.bcm.edu> Hello Histoland, Someone needs help in our lab with some fluorescent staining. He is staining drosophila retina with antibody PI4.5P2, or PIP2 for looking at Phospholipids around the membranes. He is using 4% Paraformaldehyde and Triton X in his protocol and is getting inconsistent results and the signals are weak even at the nucleus. Does anyone have a protocol or recipe that might give better results? Thank you, Lita Duraine EM Technologist Bellen Lab HHMI- Molecular Genetics Duncan Neurological Research Institute 1250 Moursund St. Houston, TX 77030 Rm: N1165.17 MS: N1125.50 832-824-8772 TEM Room 979-549-6526 Cell http://flypush.imgen.bcm.tmc.edu/lab/people/lita.php From wilson6848 <@t> yahoo.com Fri Feb 1 20:52:21 2013 From: wilson6848 <@t> yahoo.com (Wilson A) Date: Fri Feb 1 20:52:25 2013 Subject: [Histonet] HER-2 Message-ID: <1359773541.80743.YahooMailNeo@web125406.mail.ne1.yahoo.com> ? ??? Hi, ????? Our pathologists are concerned we may be reporting too many 2+ HER2?s. Can?someone??help with this? ? ???? Thanks, ??? Wilson From Steven.Weston <@t> utas.edu.au Sat Feb 2 18:48:12 2013 From: Steven.Weston <@t> utas.edu.au (Steven Weston) Date: Sat Feb 2 18:48:21 2013 Subject: [Histonet] re vacuoles in nuclei of PT Link treated tissue Message-ID: <7B808A2E6BDDBD4EA4FA6395FF7BED2B2B142604@MBXSBYN4.utas.ad.internal> Hi everyone, I have recently encountered an unusual result in our immunostaining. We have been bvery successfully using Dako's PT link with the High PH soln to dewax and heat retrieve some of our immuno samples. In conjunction with my colleagues from the local Hospital Anat Path dept we noticed a strange but prominent vacuolisation showing in the Nuclei (and less so in cytoplasm) in our treated tissue after staining with Haematoxylin. Here is the interesting thing, when we manually dewax the same type of sample and then through the high PH, PT link the vacuoles no longer appear. This was noticed in both laboratories. the first thought was that it had something to do with the wax. But we both use different waxes (precision cut and paraplast). Clearly it is something to do with the wax removal process but coming up with an explanation is challenging. Any ideas? Has anyone else noticed this phenomenon ? regards steve weston lab manager resp research group MRI From wilson6848 <@t> yahoo.com Sat Feb 2 23:20:34 2013 From: wilson6848 <@t> yahoo.com (Wilson A) Date: Sat Feb 2 23:20:38 2013 Subject: [Histonet] Her-2 procedure Message-ID: <1359868834.31548.YahooMailNeo@web125402.mail.ne1.yahoo.com> ? ? ?Hi, ???? Please I will appreciate it, if you guys could share your HER-2? PROCEDURE with me. ? Thanks, Wilson From Nancy_Schmitt <@t> pa-ucl.com Sun Feb 3 06:48:24 2013 From: Nancy_Schmitt <@t> pa-ucl.com (Nancy Schmitt) Date: Sun Feb 3 06:48:29 2013 Subject: [Histonet] congo red Message-ID: Hi to all- What is your process for pretreating congo red smears? Are you fixing them? We are running the stain on the Dako Artisan staining system. Thanks Nancy NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From rjbuesa <@t> yahoo.com Sun Feb 3 09:40:05 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sun Feb 3 09:40:09 2013 Subject: [Histonet] HER-2 In-Reply-To: <1359773541.80743.YahooMailNeo@web125406.mail.ne1.yahoo.com> References: <1359773541.80743.YahooMailNeo@web125406.mail.ne1.yahoo.com> Message-ID: <1359906005.63943.YahooMailNeo@web163102.mail.bf1.yahoo.com> That is a very strange question by your pathologists, since they are the ones doing the reporting. If they follow the reporting guidelines and arrive to a 2+ result, they should report it. Another question would be: are our lab's 2+ reports above the "mean" for other labs? The answer to this?question can be done only if your find out the 2+ percentage for all labs is, and compare with yours. I think that your pathologists should contact CAP to find out if there are statistics on this issue. Ren? J. From: Wilson A To: "histonet@lists.utsouthwestern.edu" Sent: Friday, February 1, 2013 9:52 PM Subject: [Histonet] HER-2 ? ??? Hi, ????? Our pathologists are concerned we may be reporting too many 2+ HER2?s.? Can?someone??help with this? ? ???? Thanks, ??? Wilson _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Sun Feb 3 09:41:47 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sun Feb 3 09:41:50 2013 Subject: [Histonet] Her-2 procedure In-Reply-To: <1359868834.31548.YahooMailNeo@web125402.mail.ne1.yahoo.com> References: <1359868834.31548.YahooMailNeo@web125402.mail.ne1.yahoo.com> Message-ID: <1359906107.96571.YahooMailNeo@web163104.mail.bf1.yahoo.com> The protocol described by DAKO is very explicit, it produces consistent results and is the one I followed. Check it out. Ren? J. From: Wilson A To: "histonet@lists.utsouthwestern.edu" Sent: Sunday, February 3, 2013 12:20 AM Subject: [Histonet] Her-2 procedure ? ? ?Hi, ???? Please I will appreciate it, if you guys could share your HER-2? PROCEDURE with me. ? Thanks, Wilson _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Sun Feb 3 09:42:32 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sun Feb 3 09:42:36 2013 Subject: [Histonet] congo red In-Reply-To: References: Message-ID: <1359906152.76399.YahooMailNeo@web163103.mail.bf1.yahoo.com> Yes, smears should be fixed. I used NBF. Ren? J. From: Nancy Schmitt To: "Histonet (histonet@lists.utsouthwestern.edu)" Sent: Sunday, February 3, 2013 7:48 AM Subject: [Histonet] congo red Hi to all- What is your process for pretreating congo red smears?? Are you fixing them? We are running the stain on the Dako Artisan staining system. Thanks Nancy NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Sun Feb 3 09:57:02 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sun Feb 3 09:57:06 2013 Subject: [Histonet] re vacuoles in nuclei of PT Link treated tissue In-Reply-To: <7B808A2E6BDDBD4EA4FA6395FF7BED2B2B142604@MBXSBYN4.utas.ad.internal> References: <7B808A2E6BDDBD4EA4FA6395FF7BED2B2B142604@MBXSBYN4.utas.ad.internal> Message-ID: <1359907022.454.YahooMailNeo@web163103.mail.bf1.yahoo.com> The fact that the vacuoles no longer appear when you use manual dewaxing seem to point the problem at the DAKO product. I think that you will be better off consulting with DAKO because whatever we in HistoNet could tell you would be completely speculative and probably of little help, unless somebody having the same experience found the solution. Ren? J. From: Steven Weston To: "histonet@lists.utsouthwestern.edu" Sent: Saturday, February 2, 2013 7:48 PM Subject: [Histonet] re vacuoles in nuclei of PT Link treated tissue Hi everyone, I have recently encountered an unusual result in our immunostaining. We have been bvery successfully using Dako's PT link with the High PH soln to dewax and heat retrieve some of our immuno samples. In conjunction with my colleagues from the local Hospital Anat Path dept we noticed a strange but prominent vacuolisation showing in the Nuclei (and less so in cytoplasm) in our treated tissue after staining with Haematoxylin. Here is the interesting thing, when we manually dewax the same type of sample and then through the high PH, PT link the vacuoles no longer appear. This was noticed in both laboratories. the first thought was that it had something to do with the wax. But we both use different waxes (precision cut and paraplast). Clearly it is something to do with the wax removal process but coming up with an explanation is challenging. Any ideas? Has anyone else noticed this phenomenon ? regards steve weston lab manager resp research group MRI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Stacy.Giroux <@t> stjohn.org Sun Feb 3 12:37:54 2013 From: Stacy.Giroux <@t> stjohn.org (Giroux, Stacy) Date: Sun Feb 3 12:38:00 2013 Subject: [Histonet] H&E Stainer Protocol Message-ID: <29CCF3EC44815745864D9F84A1ED4B51B433E20A20@AUSP03VMBX11.apptixhealth.net> Hi Everyone, I was wondering if anyone out there is using a Sakura DRS-601 Diversified H&E Stainer. We are having trouble setting up an appropriate H&E staining protocol to have stains come out correctly. If anyone uses this instrument and is willing to share their protocol / times / solutions etc. it would be greatly appreciated. Thank you very much for your help! Stacy Giroux, HTL(ASCP) CONFIDENTIALITY NOTICE: This email message and any accompanying data or files is confidential and may contain privileged information intended only for the named recipient(s). If you are not the intended recipient(s), you are hereby notified that the dissemination, distribution, and or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at the email address above, delete this email from your computer, and destroy any copies in any form immediately. Receipt by anyone other than the named recipient(s) is not a waiver of any attorney-client, work product, or other applicable privilege. From jnocito <@t> satx.rr.com Sun Feb 3 16:17:26 2013 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Sun Feb 3 16:24:31 2013 Subject: [Histonet] unsubscribe Message-ID: <009801ce025c$400095b0$c001c110$@rr.com> Joe Nocito, BS, PACM, HTCM (ASCP) QIHC Dept of Pathology/ 59LSQ/SGVLH Lackland AFB, TX 78236 joseph.nocito@us.af.mil From olivepitt <@t> rocketmail.com Mon Feb 4 00:40:25 2013 From: olivepitt <@t> rocketmail.com (Olive Pitt) Date: Mon Feb 4 00:40:28 2013 Subject: [Histonet] (no subject) Message-ID: <1359960025.46766.YahooMailNeo@web120606.mail.ne1.yahoo.com> http://www.blulobster.ca/tfvbbmcz.html From foreightl <@t> gmail.com Mon Feb 4 08:26:00 2013 From: foreightl <@t> gmail.com (Patrick Laurie) Date: Mon Feb 4 08:26:07 2013 Subject: [Histonet] No expiration dates on chemicals In-Reply-To: <631955447A364B45B9458D2905635110655BE248@WIN08-MBX-01.wtbyhosp.org> References: <631955447A364B45B9458D2905635110655BE248@WIN08-MBX-01.wtbyhosp.org> Message-ID: I was supervisor of a lab where we had old staining powders that were close to 30 years old. All of our other chemicals had expiration dates, but not these. CAP cited us, we sent this article in: Stain and dye stability over a 30-year period: a comparison of certified dye powders by the Biological Stain Commission DP Penney, M Frank, C Fagan, C Willis Biotechnic & Histochemistry 2009, 84(1): 1115. And they expunged the citation. This is not an official advice, your experience with this may vary. Good luck, Patrick On Thu, Jan 31, 2013 at 9:36 AM, PicheGrocki, Jessica < jpiche-grocki@wtbyhosp.org> wrote: > Hi All, > > Just wondering what everyone is doing in regards the ANP question > involving expiration dates on chemicals and reagents? Is everyone getting > rid of old chemicals that have been opened for 10 years or more? > > Please advise. > > Have a good day and thank you, > > Jessica Piche', HT (ASCP) > Waterbury Hospital Histology Lab > > > > CONFIDENTIALITY NOTICE: This email and any attachments contain > confidential information that is legally privileged. This information is > intended only for the use of the individual or entity named above. The > authorized recipient of this information is prohibited from disclosing this > information to any other party unless required to do so by law or > regulation. If you are not the intended recipient, you are hereby notified > that any disclosure, copying, distribution or action taken in reliance on > the contents of these documents is strictly prohibited. If you have > received this information in error, please notify the sender immediately > and delete these documents. Copyright (c) Waterbury Hospital > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plaurie@cellnetix.com From relia1 <@t> earthlink.net Mon Feb 4 09:22:01 2013 From: relia1 <@t> earthlink.net (Pam Barker) Date: Mon Feb 4 09:22:06 2013 Subject: [Histonet] RELIA Solutions Weekly Update. 2-4-2013. Exciting Opportunities to tell you about! Message-ID: <010b01ce02eb$61fc5520$25f4ff60$@earthlink.net> Hi Histonetters!! I hope your week is getting off to a great start. I know mine is!! Over the past few days yes even this past weekend!! I have been given some exciting new opportunities to show you. The clients are ready to hire ASAP and offering excellent compensation packages. These are all permanent full time positions. Each of them has a unique characteristic that makes it NOT YOUR AVERAGE HISTOLOGY JOB. Here is a list of my current openings: HISTOLOGY MANAGEMENT: Anatomic Pathology Manager - San Francisco Bay Area Pathology Manager - Grand Rapids, MI Histology Supervisor - York, PA Lead IHC Tech - NYC, NY Lead Histotech - Staunton, VA HISTOLOGY TECHNICIAN/TECHNOLOGIST Lead IHC Tech - NYC, NY Lead Histotechnologist - Staunton, VA Histotechnician/Histotechnologist - Collegeville, PA Histotechnician/Histotechnologist - Long Island, NY FISH Tech - Long Island, NY Molecular Diagnostics Tech - Long Island, NY Histology Tech - Charlotte, NC HT/HTL or elig - San Francisco Bay Area HT/HTL - Concord, NH Histology Tech - Lancaster/York, PA Histotechnician - Louisville, KY Histology Tech - Portland, ME More positions are coming in all of the time so if you are looking let me know where and what you are looking for and I will keep you posted. ****REMEMBER. It never hurts to look!***** If you would like to know what is so special about any or all of these positions then Let's talk. You can reach me toll free at 866-607-3542 until 6 EST and anytime on my cell phone at 407-353-5070, or shoot me an email at relia1@earthlink.net to schedule a time to talk. I really appreciate you taking the time to read this e-mail and it means a lot to me when you take the time to refer your friends and coworkers so to show my appreciation I would like to offer you a 500.00 referral fee for anyone you refer to me that I place. Have a Great Week!! Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From bamoe <@t> gundluth.org Mon Feb 4 13:31:25 2013 From: bamoe <@t> gundluth.org (Moe, Barbi A) Date: Mon Feb 4 13:33:30 2013 Subject: [Histonet] Amyloid control tissue Message-ID: <8B4B31D17067E540AC760B4A30190B99012E4B8C@LXEXMB03.gundluth.org> Does anyone know where I could purchase (or trade for) a block of amyloid positive tissue? Alternatively, for those who purchase ready-cut amyloid control slides, could you share what vendor you have been happy with? Thanks in advance! Barb Moe Gundersen Lutheran Medical Center La Crosse WI bamoe@gundluth.org From JCBRITTON <@t> Cheshire-Med.COM Mon Feb 4 13:41:22 2013 From: JCBRITTON <@t> Cheshire-Med.COM (Britton, Josette C) Date: Mon Feb 4 13:41:27 2013 Subject: [Histonet] Amyloid control tissue In-Reply-To: <8B4B31D17067E540AC760B4A30190B99012E4B8C@LXEXMB03.gundluth.org> Message-ID: American Master Tech 800-860-4073 Josie Britton HT QIHC (ASCP) Cheshire Medical Center Keene, NH -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Moe, Barbi A Sent: Monday, February 04, 2013 2:31 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Amyloid control tissue Does anyone know where I could purchase (or trade for) a block of amyloid positive tissue? Alternatively, for those who purchase ready-cut amyloid control slides, could you share what vendor you have been happy with? Thanks in advance! Barb Moe Gundersen Lutheran Medical Center La Crosse WI bamoe@gundluth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From tkngflght <@t> yahoo.com Mon Feb 4 15:05:55 2013 From: tkngflght <@t> yahoo.com (Cheryl) Date: Mon Feb 4 15:06:00 2013 Subject: [Histonet] Joe Nocito's request -- oh my! Message-ID: <1360011955.45960.YahooMailClassic@web161906.mail.bf1.yahoo.com> Oh no, Joe!? Say it ain't so!!? Please tell us this is just to take off your military address and you aren't leaving us forever... ? Cheryl Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab by helping one GREAT?Tech at a time.? 281.852.9457?Office 800.756.3309?Phone & Fax? admin@fullstaff.org Sign up for the FREE?newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please?include your name and specialty in the body of the email. --- On Mon, 2/4/13, histonet-request@lists.utsouthwestern.edu wrote: From: histonet-request@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 111, Issue 4 To: histonet@lists.utsouthwestern.edu Date: Monday, February 4, 2013, 1:05 PM Send Histonet mailing list submissions to ??? histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit ??? http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to ??? histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at ??? histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: ???1. H&E Stainer Protocol (Giroux, Stacy) ???2. unsubscribe (Joe Nocito) ???3. (no subject) (Olive Pitt) ???4. Re: No expiration dates on chemicals (Patrick Laurie) ???5. RELIA Solutions Weekly Update. 2-4-2013. Exciting ? ? ? Opportunities to tell you about! (Pam Barker) ---------------------------------------------------------------------- Message: 1 Date: Sun, 3 Feb 2013 12:37:54 -0600 From: "Giroux, Stacy" Subject: [Histonet] H&E Stainer Protocol To: "histonet@lists.utsouthwestern.edu" ??? Message-ID: ??? <29CCF3EC44815745864D9F84A1ED4B51B433E20A20@AUSP03VMBX11.apptixhealth.net> ??? Content-Type: text/plain; charset="us-ascii" Hi Everyone, I was wondering if anyone out there is using a Sakura DRS-601 Diversified H&E Stainer. We are having trouble setting up an appropriate H&E staining protocol to have stains come out correctly. If anyone uses this instrument and is willing to share their protocol / times / solutions etc. it would be greatly appreciated. Thank you very much for your help! Stacy Giroux, HTL(ASCP) CONFIDENTIALITY NOTICE: This email message and any accompanying data or files is confidential and may contain privileged information intended only for the named recipient(s). If you are not the intended recipient(s), you are hereby notified that the dissemination, distribution, and or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at the email address above, delete this email from your computer, and destroy any copies in any form immediately. Receipt by anyone other than the named recipient(s) is not a waiver of any attorney-client, work product, or other applicable privilege. ------------------------------ Message: 2 Date: Sun, 3 Feb 2013 16:17:26 -0600 From: "Joe Nocito" Subject: [Histonet] unsubscribe To: Message-ID: <009801ce025c$400095b0$c001c110$@rr.com> Content-Type: text/plain;??? charset="us-ascii" Joe Nocito, BS, PACM, HTCM (ASCP) QIHC Dept of Pathology/ 59LSQ/SGVLH Lackland AFB, TX 78236 joseph.nocito@us.af.mil ------------------------------ Message: 3 Date: Sun, 3 Feb 2013 22:40:25 -0800 (PST) From: Olive Pitt Subject: [Histonet] (no subject) To: "histonet@lists.utsouthwestern.edu" ??? Message-ID: ??? <1359960025.46766.YahooMailNeo@web120606.mail.ne1.yahoo.com> Content-Type: text/plain; charset=us-ascii http://www.blulobster.ca/tfvbbmcz.html ------------------------------ Message: 4 Date: Mon, 4 Feb 2013 06:26:00 -0800 From: Patrick Laurie Subject: Re: [Histonet] No expiration dates on chemicals To: "PicheGrocki, Jessica" Cc: "histonet@lists.utsouthwestern.edu" ??? Message-ID: ??? Content-Type: text/plain; charset=ISO-8859-1 I was supervisor of a lab where we had old staining powders that were close to 30 years old.? All of our other chemicals had expiration dates, but not these.? CAP cited us, we sent this article in: Stain and dye stability over a 30-year period: a comparison of certified dye powders by the Biological Stain Commission DP Penney, M Frank, C Fagan, C Willis Biotechnic & Histochemistry 2009, 84(1): 1115. And they expunged the citation.? This is not an official advice, your experience with this may vary. Good luck, Patrick On Thu, Jan 31, 2013 at 9:36 AM, PicheGrocki, Jessica < jpiche-grocki@wtbyhosp.org> wrote: > Hi All, > > Just wondering what everyone is doing in regards the ANP? question > involving expiration dates on chemicals and reagents? Is everyone getting > rid of old chemicals that have been opened for 10 years or more? > > Please advise. > > Have a good day and thank you, > > Jessica Piche', HT (ASCP) > Waterbury Hospital Histology Lab > > > > CONFIDENTIALITY NOTICE: This email and any attachments contain > confidential information that is legally privileged. This information is > intended only for the use of the individual or entity named above. The > authorized recipient of this information is prohibited from disclosing this > information to any other party unless required to do so by law or > regulation. If you are not the intended recipient, you are hereby notified > that any disclosure, copying, distribution or action taken in reliance on > the contents of these documents is strictly prohibited. If you have > received this information in error, please notify the sender immediately > and delete these documents. Copyright (c) Waterbury Hospital > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plaurie@cellnetix.com ------------------------------ Message: 5 Date: Mon, 4 Feb 2013 10:22:01 -0500 From: "Pam Barker" Subject: [Histonet] RELIA Solutions Weekly Update. 2-4-2013. Exciting ??? Opportunities to tell you about! To: "Histonet" Message-ID: <010b01ce02eb$61fc5520$25f4ff60$@earthlink.net> Content-Type: text/plain;??? charset="us-ascii" Hi Histonetters!! I hope your week is getting off to a great start.? I know mine is!! Over the past few days yes even this past weekend!!? I have been given some exciting new opportunities to show you.? The clients are ready to hire ASAP and offering excellent compensation packages.? These are all permanent full time positions.? Each of them has a unique characteristic that makes it NOT YOUR AVERAGE HISTOLOGY JOB. Here is a list of my current openings: HISTOLOGY MANAGEMENT: Anatomic Pathology Manager - San Francisco Bay Area Pathology Manager - Grand Rapids, MI Histology Supervisor - York, PA Lead IHC Tech -? NYC, NY Lead Histotech - Staunton, VA HISTOLOGY TECHNICIAN/TECHNOLOGIST Lead IHC Tech - NYC, NY Lead Histotechnologist - Staunton, VA Histotechnician/Histotechnologist - Collegeville, PA Histotechnician/Histotechnologist - Long Island, NY FISH Tech - Long Island, NY Molecular Diagnostics Tech - Long Island, NY Histology Tech - Charlotte, NC HT/HTL or elig - San Francisco Bay Area HT/HTL - Concord, NH Histology Tech - Lancaster/York, PA Histotechnician - Louisville, KY Histology Tech - Portland, ME More positions are coming in all of the time so if you are looking let me know where and what you are looking for and I will keep you posted. ****REMEMBER. It never hurts to look!***** If you would like to know what is so special about any or all of these positions then Let's talk. You can reach me toll free at 866-607-3542 until 6 EST and anytime on my cell phone at 407-353-5070, or shoot me an email at relia1@earthlink.net to schedule a time to talk.? I really appreciate you taking the time to read this e-mail and it means a lot to me when you take the time to refer your friends and coworkers so to show my appreciation I would like to offer you a 500.00 referral fee for anyone you refer to me that I place. Have a Great Week!!? Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:? ???(407)353-5070 FAX:? ???(407)678-2788 E-mail: relia1@earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 111, Issue 4 **************************************** From bwjones <@t> uw.edu Mon Feb 4 17:38:51 2013 From: bwjones <@t> uw.edu (Brian W. Jones) Date: Mon Feb 4 17:39:22 2013 Subject: [Histonet] extensive troubleshooting of IF on fixed-then-frozen mouse brain Message-ID: <5110468B.1050607@uw.edu> Hi All, I've used Histonet many times to answer my IF/IHC questions, but this time I'm stumped. We've been using a transcardial perfusion fixation/immunofluoresence protocol for years and it has always worked. It has worked so reliably that we would often make last-minute changes---e.g., post-fix overnight instead of 4 hours---if something else came up and it would still turn out fine. Well, obviously things aren't working now or I wouldn't be pleading for help! THE PROBLEMS: Mouse brain tissue has large holes (Swiss cheese look), lacks fine structures such as axons or dendrites (looks mushy), some of the brain regions easily separate away from others (e.g., the hippocampus separates from the thalamus), and random areas of the section are not in the same focal plane as the surrounding tissue. (I tried to post images as instructed on the web site, but I can't figure that out. Please advise as I think the images would show better than I can describe.) THE PROTOCOL: Here is a detailed description of the protocol and variations I have tried while troubleshooting. Note that I have not tried every combination of every variation, and certainly I am careful to only change one thing at a time so I can make meaningful comparisons. 1) Euthanize 3 - 5-month old mouse with Beuthansia D. Wait until no longer responsive to front paw pinch and restrain animal on dissecting board. Make incision to expose thoracic cavity and lift ribcage out of the way. Heart is still beating strongly at this point. 2) Grasp heart with blunt forceps and a) make small incision in left ventricle and insert 20-g cannula, or b) insert 20-g or 25-g needle into left ventricle. 3) Using a peristaltic pump, perfuse PBS and immediately cut right atrium for outflow. Have tried PBS at either 4 C or 35 C. Have made up PBS fresh and checked pH. Have replaced tubing and used a different pump. 4) Set flow rate to a) 1.5 mL/min, b) 3 mL/min, c) 6 mL/min, or d) 10 mL/min. (Do not have a way to measure pressure.) 5) Perfuse PBS: a) 3 mL only, b) until liver blanches (never really blanches at low flow rates), or c) 15 mL. 6) PBS is either a) PBS alone, or b) PBS with heparin. 7) Perfuse with 50 mL of fixative: 4% PFA in PBS or PLP (Nakane) fixative. Note: I have tried PFA from Sigma (two different lots) or EMS (granules or 16%). Fixative is perfused at same rate and temp as PBS. 8) For 8% PFA stock: PFA is usually dissolved in water but have tried directly in PBS. Dissolved by bringing solution to 55 C then adding PFA while stirring. A few drops of 1-4 M NaOH are added until solution clears (a few undissolved particles remain). Solution is filtered through Whatman paper or 0.45 um membrane. Always made fresh the day of the perfusion. 9) Animal's lower extremities begin to move shortly after introduction of fixative. Rarely observe perfusate exiting the nose/mouth. 10) Post-fix: after perfusion, brain is dissected from skull using scissors and forceps and placed in 4 C fixative for 4-7 hours or overnight. 11) Brain is cryoprotected in sucrose-PBS: a) 10% sucrose until brain sinks, then 20%, then 30%; or b) 30% sucrose until brain sinks; c) three changes of 25% sucrose over three days total, or d) some other variation that ultimately ends in a brain sunk in at least 25% sucrose. 12) Brain is placed in 4 C Tissue-Tek OCT and frozen a) on powdered dry ice, or b) liquid nitrogen and then on dry ice while freezing other brains. 13) Brain is stored at -80 C or taken directly to cryostat. Have used two different cryostats. 14) Brains are allowed to equilibrate to cryostat temp, usually -17 C. 15) 20 um sections with new knife are a) placed thawed directly onto slide or b) placed in PBS and floated onto slide. Note: everything "feels" right on the cryostat. 16) Slides: a) VWR Superfrost, b) Fisher Superfrost Plus, or c) Superfrost Plus Gold. 17) Sections on slides are a) left to air dry for 30+ minutes, b) stored -80 C, or c) placed immediately in TBS-T. 18) Antibody procedures are done in 1% BSA in TBS-T; washes are with TBS-T. (Have tried using another lab's TBS-T as well.) 19) Slides are mounted with Prolong Gold + DAPI --- and by this time I've gone through several bottles of it, so it's never old! As mentioned above, in the past it seemed that any variation to the protocol would still work. Now, it seems that I can't make it work no matter what I change. I was convinced that it was a fixative problem, but as noted I've tried multiple sources/preparations of PFA. As my colleague put it: "You've tested all the scientific and all the superstitious factors." Thank you for any help you can provide! -- Brian Jones, Ph.D From gibi55 <@t> yahoo.com Mon Feb 4 19:31:55 2013 From: gibi55 <@t> yahoo.com (Gino Bianchi) Date: Mon Feb 4 19:31:59 2013 Subject: [Histonet] microcalcifications in breast stereo biopsies Message-ID: <1360027915.51817.YahooMailNeo@web160302.mail.bf1.yahoo.com> Hi everybody. I?m a pathologist involved in Pathology residents ?training at the Universidad Central de Venezuela, Caracas, Venezuela. Sometimes, we can?t demonstrate the presence of microcalcifications in stereotactic breast biopsies, in spite of observing them in the paraffin blocks ( x-ray) . The lab technicians cut the blocks at 50 microns interval , doing?routinely?3 levels ,and ?sectioning all the tissue when we get no micros in the slides. We have reviewed the no calcium slides with polarized light without success, and all we got is a sense of frustration. Any advice would be welcome, as well as your processing/sectioning protocols Thank you in advance, ? ? Gino Italo Bianchi Pathologist Breast Pathology Universidad Central de Venezuela, Caracas, Venezuela From ruth.muljadi <@t> monash.edu Mon Feb 4 21:42:40 2013 From: ruth.muljadi <@t> monash.edu (Ruth Muljadi) Date: Mon Feb 4 21:42:46 2013 Subject: [Histonet] FoxP3 stain on FFPE decalcified bone Message-ID: Hi, Does the standard antigen retrieval protocol need to be changed for decalcified tissue (using Formaldehyde, Formic Acid and Methanol). I would like to stain for FoxP3 on FFPE decalcified bone. Thanks, Ruth From rcartun <@t> harthosp.org Mon Feb 4 21:57:37 2013 From: rcartun <@t> harthosp.org (Richard Cartun) Date: Mon Feb 4 21:57:47 2013 Subject: [Histonet] HER-2 Message-ID: <51103CE10200007700036808@gwmail1.harthosp.org> Which primary antibody and detection system are you using? Are you testing core biopsy tissue or excisional tumor? What criteria are your pathologists using to score a tumor "2+"? Richard Richard Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collections Programs Assistant Director, Anatomic Pathology Hartford Hospital/Clinical Laboratory Partners 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-2204 Fax >>> Wilson A 02/01/13 9:56 PM >>> Hi, Our pathologists are concerned we may be reporting too many 2+ HER2?s. Can someone help with this? Thanks, Wilson _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jonathan.Cremer <@t> med.kuleuven.be Tue Feb 5 02:08:16 2013 From: Jonathan.Cremer <@t> med.kuleuven.be (Jonathan Cremer) Date: Tue Feb 5 02:08:33 2013 Subject: [Histonet] extensive troubleshooting of IF on fixed-then-frozen mouse brain In-Reply-To: <5110468B.1050607@uw.edu> References: <5110468B.1050607@uw.edu> Message-ID: You're not making your formaldehyde solution properly, that might be the first of your issues. Paraformaldehyde is the polymer of formaldehyde, and has little to no fixative properties. It doesn't dissolve in water either. To make a proper fixative from paraformaldehyde, you have to hydrolyse it in a basic solution. To make 4% formaldehyde in PBS, resuspend 40 g of paraformaldehyde in about 700 ml of water and add one or two drops of 2-3 M NaOH. Leave it stirring for 5-10 minutes, add one drop of NaOH if the solution hasn't cleared up entirely. (You can warm up the liquid to speed to process, but don't go above 60 ?C or the formaldehyde will start to decompose.) Don't overdo it with the NaOH, because you have to bring down the pH again with HCl, and the more you add, the more the salinity of the final solution will go up. When you have a clear solution, add either 100 ml of 10x PBS, or add the dry powders for 1 l of PBS. Stir to dissolve/mix, then bring the pH to 7,4 (or whichever desired) with 1 M HCl. Finally, add water to a total volume of 1 l. To my view, the PBS is there mostly to buffer the formic acid from formaldehyde decomposition. You could make up in water or saline only, but keep an eye on the pH. Also, when diluting concentrated formaldehyde (formalin and formal are just old commercial names for 37-41% of formaldehyde in water, with 10% methanol), use PBS for immediate use (the small polymers are then hydrolized rapidly) or dilute in water and leave 48 hours before use (slow depolymerization). --- Jonathan Cremer Laboratory Technician TARGID - KU Leuven Gasthuisberg CDG; Labo Experimentele Immunologie; Herestraat 49 bus 811; 3000 Leuven; Belgium ________________________________________ Van: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] namens Brian W. Jones [bwjones@uw.edu] Verzonden: dinsdag 5 februari 2013 0:38 To: histonet@lists.utsouthwestern.edu Onderwerp: [Histonet] extensive troubleshooting of IF on fixed-then-frozen mouse brain Hi All, I've used Histonet many times to answer my IF/IHC questions, but this time I'm stumped. We've been using a transcardial perfusion fixation/immunofluoresence protocol for years and it has always worked. It has worked so reliably that we would often make last-minute changes---e.g., post-fix overnight instead of 4 hours---if something else came up and it would still turn out fine. Well, obviously things aren't working now or I wouldn't be pleading for help! THE PROBLEMS: Mouse brain tissue has large holes (Swiss cheese look), lacks fine structures such as axons or dendrites (looks mushy), some of the brain regions easily separate away from others (e.g., the hippocampus separates from the thalamus), and random areas of the section are not in the same focal plane as the surrounding tissue. (I tried to post images as instructed on the web site, but I can't figure that out. Please advise as I think the images would show better than I can describe.) THE PROTOCOL: Here is a detailed description of the protocol and variations I have tried while troubleshooting. Note that I have not tried every combination of every variation, and certainly I am careful to only change one thing at a time so I can make meaningful comparisons. 1) Euthanize 3 - 5-month old mouse with Beuthansia D. Wait until no longer responsive to front paw pinch and restrain animal on dissecting board. Make incision to expose thoracic cavity and lift ribcage out of the way. Heart is still beating strongly at this point. 2) Grasp heart with blunt forceps and a) make small incision in left ventricle and insert 20-g cannula, or b) insert 20-g or 25-g needle into left ventricle. 3) Using a peristaltic pump, perfuse PBS and immediately cut right atrium for outflow. Have tried PBS at either 4 C or 35 C. Have made up PBS fresh and checked pH. Have replaced tubing and used a different pump. 4) Set flow rate to a) 1.5 mL/min, b) 3 mL/min, c) 6 mL/min, or d) 10 mL/min. (Do not have a way to measure pressure.) 5) Perfuse PBS: a) 3 mL only, b) until liver blanches (never really blanches at low flow rates), or c) 15 mL. 6) PBS is either a) PBS alone, or b) PBS with heparin. 7) Perfuse with 50 mL of fixative: 4% PFA in PBS or PLP (Nakane) fixative. Note: I have tried PFA from Sigma (two different lots) or EMS (granules or 16%). Fixative is perfused at same rate and temp as PBS. 8) For 8% PFA stock: PFA is usually dissolved in water but have tried directly in PBS. Dissolved by bringing solution to 55 C then adding PFA while stirring. A few drops of 1-4 M NaOH are added until solution clears (a few undissolved particles remain). Solution is filtered through Whatman paper or 0.45 um membrane. Always made fresh the day of the perfusion. 9) Animal's lower extremities begin to move shortly after introduction of fixative. Rarely observe perfusate exiting the nose/mouth. 10) Post-fix: after perfusion, brain is dissected from skull using scissors and forceps and placed in 4 C fixative for 4-7 hours or overnight. 11) Brain is cryoprotected in sucrose-PBS: a) 10% sucrose until brain sinks, then 20%, then 30%; or b) 30% sucrose until brain sinks; c) three changes of 25% sucrose over three days total, or d) some other variation that ultimately ends in a brain sunk in at least 25% sucrose. 12) Brain is placed in 4 C Tissue-Tek OCT and frozen a) on powdered dry ice, or b) liquid nitrogen and then on dry ice while freezing other brains. 13) Brain is stored at -80 C or taken directly to cryostat. Have used two different cryostats. 14) Brains are allowed to equilibrate to cryostat temp, usually -17 C. 15) 20 um sections with new knife are a) placed thawed directly onto slide or b) placed in PBS and floated onto slide. Note: everything "feels" right on the cryostat. 16) Slides: a) VWR Superfrost, b) Fisher Superfrost Plus, or c) Superfrost Plus Gold. 17) Sections on slides are a) left to air dry for 30+ minutes, b) stored -80 C, or c) placed immediately in TBS-T. 18) Antibody procedures are done in 1% BSA in TBS-T; washes are with TBS-T. (Have tried using another lab's TBS-T as well.) 19) Slides are mounted with Prolong Gold + DAPI --- and by this time I've gone through several bottles of it, so it's never old! As mentioned above, in the past it seemed that any variation to the protocol would still work. Now, it seems that I can't make it work no matter what I change. I was convinced that it was a fixative problem, but as noted I've tried multiple sources/preparations of PFA. As my colleague put it: "You've tested all the scientific and all the superstitious factors." Thank you for any help you can provide! -- Brian Jones, Ph.D _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Tue Feb 5 06:16:01 2013 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/OID/NCEZID)) Date: Tue Feb 5 06:16:12 2013 Subject: [Histonet] H&E/LFB combination Message-ID: Morning all, I have been asked to do this technique and just wanted to know from those of you that have done it....any tricks or do you basically do the 2 protocols back-to-back ? Thanks, Jeanine H. Bartlett Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 404-639-3590 jeanine.bartlett@cdc.hhs.gov From MMargiotta <@t> bmhmc.org Tue Feb 5 07:59:05 2013 From: MMargiotta <@t> bmhmc.org (Margiotta-Watz, Michele) Date: Tue Feb 5 07:59:10 2013 Subject: [Histonet] cassette labelers Message-ID: <230D0B9EC57D7A45A7A186C6AB4C7ABC296E1278@BMH-EXCHANGE-01.BMHMC.ORG> Hi All, We are looking into purchasing a cassette labeler for our lab. Wondering what other labs are using. Any feedback is appreciated! Thanks, Michele Histology Supervisor BMHMC 631-654-7192 DISCLAIMER: This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. From ltougas <@t> dawsoncollege.qc.ca Tue Feb 5 11:53:48 2013 From: ltougas <@t> dawsoncollege.qc.ca (Liette Tougas) Date: Tue Feb 5 11:53:53 2013 Subject: [Histonet] standards for microtomy bench In-Reply-To: References: <455897B94CF3A44F81F727160F23FB110C7E996E@DC229.ad.dawsoncollege.qc.ca>, Message-ID: <455897B94CF3A44F81F727160F23FB110C7F7927@DC229.ad.dawsoncollege.qc.ca> Thank you so much for your advices, Benjamin! Very helpful even if, in reality, not all suggestions may be accomodated. Regards, Liette Tougas, RT, B.Sc., M.Sc. Biomedical Laboratory Technology Department Dawson College 514-931-8731, ext 1519 ________________________________ From: Benjamin Ferland [benjamin@histologistics.com] Sent: February 4, 2013 7:37 PM To: Liette Tougas Subject: Re: [Histonet] standards for microtomy bench Make the workspace have plenty of leg room on both sides and in front. Most histologist prefer to sit in a desk chair with wheels to allow them to spin from side to side, cramped legs can be bothersome. Also make sure the workspace can be converted to left handed use, dont but it up into a corner. It is nice to have a cold plate from an embedding center on one side of the microtome, and a waterbath on the other side of the microtome. The most important things are light, and air. Do not put a vent of any sort around the microtome, it will make it impossible to work at. The air needs to be still so that the parraffin ribbons do not go flying off. Some techs get angry if you walk by them fast and create wind lol, they have called me "hurricane Ben" and "the human tornado" for walking to fast when I am in a hurry. Lots of light is good, from above. Using a desk lamp cast lots of shadows and makes it hard on the eyes. It is best to have fixtures in the ceiling above each tech. I have created several workspaces now since becoming a histologist. The best one I created was in front of a window. The natural light was great, with a nice blind to pull down when the sun got to intense. The worst design I have seen had a cabinet above the workstation, so it felt very confined. Also if there are multiple histologist, it is good to sit across from each other so you can comunicate better without stopping work. More of a dinner table sort of layout. Sorry for any typos I wrote this very quickly, dinner is almost ready but I was very interested in your post. If you look at masshistology.com there are some photos under the "new lab photos" of the workstations with the windows. That is a lab I used to work at, I helped with the design when they moved to a new facility years ago. The do not use the cold plates, they use other things to chill the blocks but the idea is the same. Four feet to five feet across should be good with a 30 inch depth, mabye more depending on the equipment. On Thu, Jan 31, 2013 at 4:22 PM, Liette Tougas > wrote: Hi everyone, I would like to know if anyone has standards (from any country!) regarding the ideal amount of space for a microtomy workbench eg: * If facing a wall: dept usually required. * Width of individual counter space which would include the microtome, floatation bath, rack for cut sections. (blocks kept cold in fridgitray nearby) * Suggested dept underneath working counter (towards the back for leg room) Any other suggestion or elements to take into account when designing microtomy benches? Thank you very much in advance for any precious advice! Liette Tougas, RT, B.Sc., M.Sc. , faculty Biomedical Laboratory Technology Department Dawson College, Montreal, Qc _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Benjamin Ferland HT (ASCP) 617-710-4870 Benjamin@histologistics.com From Nancy_Schmitt <@t> pa-ucl.com Tue Feb 5 12:20:06 2013 From: Nancy_Schmitt <@t> pa-ucl.com (Nancy Schmitt) Date: Tue Feb 5 12:20:13 2013 Subject: [Histonet] cassette labelers In-Reply-To: <20130205180310.DE73B1AA036@mail.pa-ucl.com> References: <20130205180310.DE73B1AA036@mail.pa-ucl.com> Message-ID: <906B4DA90ED1DB4DB6C7E94D7CEE6C364DC6BAE2@PEITHA.wad.pa-ucl.com> Michele- We went with General Data - the cassette labeler is up and running very nicely -next phase is slide labeling. Nancy United Clinical Laboratories Dubuque, IA -------------------------------------------------------------------- Message: 10 Date: Tue, 5 Feb 2013 13:59:05 +0000 From: "Margiotta-Watz, Michele" Subject: [Histonet] cassette labelers To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <230D0B9EC57D7A45A7A186C6AB4C7ABC296E1278@BMH-EXCHANGE-01.BMHMC.ORG> Content-Type: text/plain; charset="us-ascii" Hi All, We are looking into purchasing a cassette labeler for our lab. Wondering what other labs are using. Any feedback is appreciated! Thanks, Michele Histology Supervisor BMHMC 631-654-7192 DISCLAIMER: NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From rsrichmond <@t> gmail.com Tue Feb 5 12:54:59 2013 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Tue Feb 5 12:55:05 2013 Subject: [Histonet] Re: Amyloid control tissue Message-ID: Barb Moe at Gundersen Lutheran Medical Center in La Crosse, Wisconsin asks: >>Does anyone know where I could purchase (or trade for) a block of amyloid positive tissue? Alternatively, for those who purchase ready-cut amyloid control slides, could you share what vendor you have been happy with?<< If your hospital's tumor registry can find you a case of medullary carcinoma of the thyroid, most of these contain an amyloid, and I've seen a number of them used as amyloid control slides. Supposedly paraffin sections once cut must be used within a month or they go bad, so you really should have a paraffin block. (Does anyone know if this is an urban legend?) I've asked this question many times and never had any response to it. It's quite easy to produce amyloidosis in experimental animals - injecting mice with casein is a standard technique. Has anybody ever used this animal material as a control in human pathology? I actually know where La Crosse WI is - we go by there when we drive up to Wabasha MN to visit my wife's sisters. Just across the Mississippi is La Crescent, and up the road a way is La Mohel (not sure I spelled that right, but it does strike you as delightfully ecumenical). Bob Richmond Samurai Pathologist Maryville TN From marktarango <@t> gmail.com Tue Feb 5 13:24:44 2013 From: marktarango <@t> gmail.com (Mark Tarango) Date: Tue Feb 5 13:24:48 2013 Subject: [Histonet] HER-2 In-Reply-To: <1359773541.80743.YahooMailNeo@web125406.mail.ne1.yahoo.com> References: <1359773541.80743.YahooMailNeo@web125406.mail.ne1.yahoo.com> Message-ID: I'd be interested to know if all your pathologists agree that the 2+ cases are 2+. Is it possible that one of the pathologists is calling more cases as 2+ than the rest? I would have a lot of questions before modifying the staining protocol. It would be helpful you posted more info. Mark On Fri, Feb 1, 2013 at 6:52 PM, Wilson A wrote: > > Hi, > Our pathologists are concerned we may be reporting too many 2+ > HER2?s. Can someone help with this? > > Thanks, > Wilson > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From marktarango <@t> gmail.com Tue Feb 5 13:42:09 2013 From: marktarango <@t> gmail.com (Mark Tarango) Date: Tue Feb 5 13:42:12 2013 Subject: [Histonet] Joe Nocito's request -- oh my! In-Reply-To: <1360011955.45960.YahooMailClassic@web161906.mail.bf1.yahoo.com> References: <1360011955.45960.YahooMailClassic@web161906.mail.bf1.yahoo.com> Message-ID: Am I the only one who thinks its funny Joe the Toe did an unsubscribe e-mail and Cheryl Kerry copied an entire digest to reply. Oh histonet pet peeves... haha On Mon, Feb 4, 2013 at 1:05 PM, Cheryl wrote: > Oh no, Joe! Say it ain't so!! Please tell us this is just to take off > your military address and you aren't leaving us forever... > > Cheryl > > > Cheryl Kerry, HT(ASCP) > Full Staff Inc. > Staffing the AP Lab by helping one GREAT Tech at a time. > 281.852.9457 Office > 800.756.3309 Phone & Fax > admin@fullstaff.org > > Sign up for the FREE newsletter AP News--updates, tricks of the trade and > current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' > request to APNews@fullstaff.org. Please include your name and specialty > in the body of the email. > > --- On Mon, 2/4/13, histonet-request@lists.utsouthwestern.edu < > histonet-request@lists.utsouthwestern.edu> wrote: From pow.joshi <@t> gmail.com Tue Feb 5 13:42:52 2013 From: pow.joshi <@t> gmail.com (Pow Joshi) Date: Tue Feb 5 13:43:18 2013 Subject: [Histonet] Re: Histonet Digest, Vol 111, Issue 5 In-Reply-To: <511148e9.499ab60a.01b9.ffff888aSMTPIN_ADDED_MISSING@mx.google.com> References: <511148e9.499ab60a.01b9.ffff888aSMTPIN_ADDED_MISSING@mx.google.com> Message-ID: Hi Brian, I am wondering three possibilities: 1. the PFA that you use, powder form, is rather old. ( however, you said that you have tried other powders and concoctions that didn't work as well!)... perhaps, you could try using Polysciences Formaldehyde. The sealed vials come as 16% stock that you can open and dilute immediately. 2. The procedure: For some reason, the fixative isn't reaching the brain well enough. The question to you is, do you see clear, whitish coloured brain after you dissect or would you see it reddish or pinkish in colour. The reason could be a. the heart stopped beating very early in your procedure. Perhaps, slightly lower the dose or Avertin/anaesthetic. (I have had this issue previously, but it almost always works if the brain sits in the fixative unless it is never fixed! Or, the needle is not positioned correctly in the left ventricle, and fixative did not reach circulation. 3.Fixation itself: I Have had issues with the dendrites looking bloated and nobby with swiss cheese morphology before with only PFA fixation. These, however, were slice cultures, so different from whole brain fixations. They apparently needed 4% sucrose in the fixative to take care of the However, I would definitely check that. I would suggest adding 4% sucrose to the fixative as well and see if it improves the morphology. Please let us know what worked for you when you solve the issue!!! Thanks and Best, Pow Message: 4 > Date: Mon, 04 Feb 2013 15:38:51 -0800 > From: "Brian W. Jones" > Subject: [Histonet] extensive troubleshooting of IF on > fixed-then-frozen mouse brain > To: histonet@lists.utsouthwestern.edu > Message-ID: <5110468B.1050607@uw.edu> > Content-Type: text/plain; charset=ISO-8859-1; format=flowed > > Hi All, > > I've used Histonet many times to answer my IF/IHC questions, but this > time I'm stumped. We've been using a transcardial perfusion > fixation/immunofluoresence protocol for years and it has always worked. > It has worked so reliably that we would often make last-minute > changes---e.g., post-fix overnight instead of 4 hours---if something > else came up and it would still turn out fine. > > Well, obviously things aren't working now or I wouldn't be pleading for > help! > > THE PROBLEMS: > Mouse brain tissue has large holes (Swiss cheese look), lacks fine > structures such as axons or dendrites (looks mushy), some of the brain > regions easily separate away from others (e.g., the hippocampus > separates from the thalamus), and random areas of the section are not in > the same focal plane as the surrounding tissue. (I tried to post images > as instructed on the web site, but I can't figure that out. Please > advise as I think the images would show better than I can describe.) > > THE PROTOCOL: > Here is a detailed description of the protocol and variations I have > tried while troubleshooting. Note that I have not tried every > combination of every variation, and certainly I am careful to only > change one thing at a time so I can make meaningful comparisons. > > 1) Euthanize 3 - 5-month old mouse with Beuthansia D. Wait until no > longer responsive to front paw pinch and restrain animal on dissecting > board. Make incision to expose thoracic cavity and lift ribcage out of > the way. Heart is still beating strongly at this point. > 2) Grasp heart with blunt forceps and a) make small incision in left > ventricle and insert 20-g cannula, or b) insert 20-g or 25-g needle into > left ventricle. > 3) Using a peristaltic pump, perfuse PBS and immediately cut right > atrium for outflow. Have tried PBS at either 4 C or 35 C. Have made up > PBS fresh and checked pH. Have replaced tubing and used a different pump. > 4) Set flow rate to a) 1.5 mL/min, b) 3 mL/min, c) 6 mL/min, or d) 10 > mL/min. (Do not have a way to measure pressure.) > 5) Perfuse PBS: a) 3 mL only, b) until liver blanches (never really > blanches at low flow rates), or c) 15 mL. > 6) PBS is either a) PBS alone, or b) PBS with heparin. > 7) Perfuse with 50 mL of fixative: 4% PFA in PBS or PLP (Nakane) > fixative. Note: I have tried PFA from Sigma (two different lots) or EMS > (granules or 16%). Fixative is perfused at same rate and temp as PBS. > 8) For 8% PFA stock: PFA is usually dissolved in water but have tried > directly in PBS. Dissolved by bringing solution to 55 C then adding PFA > while stirring. A few drops of 1-4 M NaOH are added until solution > clears (a few undissolved particles remain). Solution is filtered > through Whatman paper or 0.45 um membrane. Always made fresh the day of > the perfusion. > 9) Animal's lower extremities begin to move shortly after introduction > of fixative. Rarely observe perfusate exiting the nose/mouth. > 10) Post-fix: after perfusion, brain is dissected from skull using > scissors and forceps and placed in 4 C fixative for 4-7 hours or overnight. > 11) Brain is cryoprotected in sucrose-PBS: a) 10% sucrose until brain > sinks, then 20%, then 30%; or b) 30% sucrose until brain sinks; c) three > changes of 25% sucrose over three days total, or d) some other variation > that ultimately ends in a brain sunk in at least 25% sucrose. > 12) Brain is placed in 4 C Tissue-Tek OCT and frozen a) on powdered dry > ice, or b) liquid nitrogen and then on dry ice while freezing other brains. > 13) Brain is stored at -80 C or taken directly to cryostat. Have used > two different cryostats. > 14) Brains are allowed to equilibrate to cryostat temp, usually -17 C. > 15) 20 um sections with new knife are a) placed thawed directly onto > slide or b) placed in PBS and floated onto slide. Note: everything > "feels" right on the cryostat. > 16) Slides: a) VWR Superfrost, b) Fisher Superfrost Plus, or c) > Superfrost Plus Gold. > 17) Sections on slides are a) left to air dry for 30+ minutes, b) stored > -80 C, or c) placed immediately in TBS-T. > 18) Antibody procedures are done in 1% BSA in TBS-T; washes are with > TBS-T. (Have tried using another lab's TBS-T as well.) > 19) Slides are mounted with Prolong Gold + DAPI --- and by this time > I've gone through several bottles of it, so it's never old! > > As mentioned above, in the past it seemed that any variation to the > protocol would still work. Now, it seems that I can't make it work no > matter what I change. I was convinced that it was a fixative problem, > but as noted I've tried multiple sources/preparations of PFA. As my > colleague put it: "You've tested all the scientific and all the > superstitious factors." > > Thank you for any help you can provide! > > -- > Brian Jones, Ph.D > > > Message: 8 > Date: Tue, 5 Feb 2013 08:08:16 +0000 > From: Jonathan Cremer > Subject: RE: [Histonet] extensive troubleshooting of IF on > fixed-then-frozen mouse brain > To: "Brian W. Jones" , > "histonet@lists.utsouthwestern.edu" > > Message-ID: > < > C35C2709C22FC2458D6A918FACC338A30FA7A9C6@ICTS-S-MBX5.luna.kuleuven.be> > > Content-Type: text/plain; charset="iso-8859-1" > > You're not making your formaldehyde solution properly, that might be the > first of your issues. > Paraformaldehyde is the polymer of formaldehyde, and has little to no > fixative properties. It doesn't dissolve in water either. To make a proper > fixative from paraformaldehyde, you have to hydrolyse it in a basic > solution. > > To make 4% formaldehyde in PBS, resuspend 40 g of paraformaldehyde in > about 700 ml of water and add one or two drops of 2-3 M NaOH. Leave it > stirring for 5-10 minutes, add one drop of NaOH if the solution hasn't > cleared up entirely. (You can warm up the liquid to speed to process, but > don't go above 60 ?C or the formaldehyde will start to decompose.) Don't > overdo it with the NaOH, because you have to bring down the pH again with > HCl, and the more you add, the more the salinity of the final solution will > go up. > When you have a clear solution, add either 100 ml of 10x PBS, or add the > dry powders for 1 l of PBS. Stir to dissolve/mix, then bring the pH to 7,4 > (or whichever desired) with 1 M HCl. Finally, add water to a total volume > of 1 l. > > To my view, the PBS is there mostly to buffer the formic acid from > formaldehyde decomposition. You could make up in water or saline only, but > keep an eye on the pH. > > Also, when diluting concentrated formaldehyde (formalin and formal are > just old commercial names for 37-41% of formaldehyde in water, with 10% > methanol), use PBS for immediate use (the small polymers are then > hydrolized rapidly) or dilute in water and leave 48 hours before use (slow > depolymerization). > --- > Jonathan Cremer > Laboratory Technician > TARGID - KU Leuven > > Gasthuisberg CDG; Labo Experimentele Immunologie; Herestraat 49 bus 811; > 3000 Leuven; Belgium > > ________________________________________ > Van: histonet-bounces@lists.utsouthwestern.edu [ > histonet-bounces@lists.utsouthwestern.edu] namens Brian W. Jones [ > bwjones@uw.edu] > Verzonden: dinsdag 5 februari 2013 0:38 > To: histonet@lists.utsouthwestern.edu > Onderwerp: [Histonet] extensive troubleshooting of IF on fixed-then-frozen > mouse brain > > Hi All, > > I've used Histonet many times to answer my IF/IHC questions, but this > time I'm stumped. We've been using a transcardial perfusion > fixation/immunofluoresence protocol for years and it has always worked. > It has worked so reliably that we would often make last-minute > changes---e.g., post-fix overnight instead of 4 hours---if something > else came up and it would still turn out fine. > > Well, obviously things aren't working now or I wouldn't be pleading for > help! > > THE PROBLEMS: > Mouse brain tissue has large holes (Swiss cheese look), lacks fine > structures such as axons or dendrites (looks mushy), some of the brain > regions easily separate away from others (e.g., the hippocampus > separates from the thalamus), and random areas of the section are not in > the same focal plane as the surrounding tissue. (I tried to post images > as instructed on the web site, but I can't figure that out. Please > advise as I think the images would show better than I can describe.) > > THE PROTOCOL: > Here is a detailed description of the protocol and variations I have > tried while troubleshooting. Note that I have not tried every > combination of every variation, and certainly I am careful to only > change one thing at a time so I can make meaningful comparisons. > > 1) Euthanize 3 - 5-month old mouse with Beuthansia D. Wait until no > longer responsive to front paw pinch and restrain animal on dissecting > board. Make incision to expose thoracic cavity and lift ribcage out of > the way. Heart is still beating strongly at this point. > 2) Grasp heart with blunt forceps and a) make small incision in left > ventricle and insert 20-g cannula, or b) insert 20-g or 25-g needle into > left ventricle. > 3) Using a peristaltic pump, perfuse PBS and immediately cut right > atrium for outflow. Have tried PBS at either 4 C or 35 C. Have made up > PBS fresh and checked pH. Have replaced tubing and used a different pump. > 4) Set flow rate to a) 1.5 mL/min, b) 3 mL/min, c) 6 mL/min, or d) 10 > mL/min. (Do not have a way to measure pressure.) > 5) Perfuse PBS: a) 3 mL only, b) until liver blanches (never really > blanches at low flow rates), or c) 15 mL. > 6) PBS is either a) PBS alone, or b) PBS with heparin. > 7) Perfuse with 50 mL of fixative: 4% PFA in PBS or PLP (Nakane) > fixative. Note: I have tried PFA from Sigma (two different lots) or EMS > (granules or 16%). Fixative is perfused at same rate and temp as PBS. > 8) For 8% PFA stock: PFA is usually dissolved in water but have tried > directly in PBS. Dissolved by bringing solution to 55 C then adding PFA > while stirring. A few drops of 1-4 M NaOH are added until solution > clears (a few undissolved particles remain). Solution is filtered > through Whatman paper or 0.45 um membrane. Always made fresh the day of > the perfusion. > 9) Animal's lower extremities begin to move shortly after introduction > of fixative. Rarely observe perfusate exiting the nose/mouth. > 10) Post-fix: after perfusion, brain is dissected from skull using > scissors and forceps and placed in 4 C fixative for 4-7 hours or overnight. > 11) Brain is cryoprotected in sucrose-PBS: a) 10% sucrose until brain > sinks, then 20%, then 30%; or b) 30% sucrose until brain sinks; c) three > changes of 25% sucrose over three days total, or d) some other variation > that ultimately ends in a brain sunk in at least 25% sucrose. > 12) Brain is placed in 4 C Tissue-Tek OCT and frozen a) on powdered dry > ice, or b) liquid nitrogen and then on dry ice while freezing other brains. > 13) Brain is stored at -80 C or taken directly to cryostat. Have used > two different cryostats. > 14) Brains are allowed to equilibrate to cryostat temp, usually -17 C. > 15) 20 um sections with new knife are a) placed thawed directly onto > slide or b) placed in PBS and floated onto slide. Note: everything > "feels" right on the cryostat. > 16) Slides: a) VWR Superfrost, b) Fisher Superfrost Plus, or c) > Superfrost Plus Gold. > 17) Sections on slides are a) left to air dry for 30+ minutes, b) stored > -80 C, or c) placed immediately in TBS-T. > 18) Antibody procedures are done in 1% BSA in TBS-T; washes are with > TBS-T. (Have tried using another lab's TBS-T as well.) > 19) Slides are mounted with Prolong Gold + DAPI --- and by this time > I've gone through several bottles of it, so it's never old! > > As mentioned above, in the past it seemed that any variation to the > protocol would still work. Now, it seems that I can't make it work no > matter what I change. I was convinced that it was a fixative problem, > but as noted I've tried multiple sources/preparations of PFA. As my > colleague put it: "You've tested all the scientific and all the > superstitious factors." > > Thank you for any help you can provide! > > -- > Brian Jones, Ph.D > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From MAUGER <@t> email.chop.edu Tue Feb 5 13:50:46 2013 From: MAUGER <@t> email.chop.edu (Mauger, Joanne) Date: Tue Feb 5 13:50:52 2013 Subject: [Histonet] Joe Nocito's request -- oh my! In-Reply-To: References: <1360011955.45960.YahooMailClassic@web161906.mail.bf1.yahoo.com> Message-ID: <929548F049BC6D448DF9488B556449BE0BD05875@EXCMBXPW5.chop.edu> You're not alone- funny! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Tarango Sent: Tuesday, February 05, 2013 2:42 PM To: Cheryl Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Joe Nocito's request -- oh my! Am I the only one who thinks its funny Joe the Toe did an unsubscribe e-mail and Cheryl Kerry copied an entire digest to reply. Oh histonet pet peeves... haha On Mon, Feb 4, 2013 at 1:05 PM, Cheryl wrote: > Oh no, Joe! Say it ain't so!! Please tell us this is just to take > off your military address and you aren't leaving us forever... > > Cheryl > > > Cheryl Kerry, HT(ASCP) > Full Staff Inc. > Staffing the AP Lab by helping one GREAT Tech at a time. > 281.852.9457 Office > 800.756.3309 Phone & Fax > admin@fullstaff.org > > Sign up for the FREE newsletter AP News--updates, tricks of the trade > and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' > request to APNews@fullstaff.org. Please include your name and > specialty in the body of the email. > > --- On Mon, 2/4/13, histonet-request@lists.utsouthwestern.edu < > histonet-request@lists.utsouthwestern.edu> wrote: _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek <@t> digestivespecialists.com Tue Feb 5 13:59:49 2013 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Tue Feb 5 14:00:34 2013 Subject: [Histonet] Joe Nocito's request -- oh my! In-Reply-To: References: <1360011955.45960.YahooMailClassic@web161906.mail.bf1.yahoo.com> Message-ID: <5A2BD13465E061429D6455C8D6B40E3916490A9A97@IBMB7Exchange.digestivespecialists.com> I figured it was a test to see who would go on a rant! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Tarango Sent: Tuesday, February 05, 2013 2:42 PM To: Cheryl Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Joe Nocito's request -- oh my! Am I the only one who thinks its funny Joe the Toe did an unsubscribe e-mail and Cheryl Kerry copied an entire digest to reply. Oh histonet pet peeves... haha On Mon, Feb 4, 2013 at 1:05 PM, Cheryl wrote: > Oh no, Joe! Say it ain't so!! Please tell us this is just to take > off your military address and you aren't leaving us forever... > > Cheryl > > > Cheryl Kerry, HT(ASCP) > Full Staff Inc. > Staffing the AP Lab by helping one GREAT Tech at a time. > 281.852.9457 Office > 800.756.3309 Phone & Fax > admin@fullstaff.org > > Sign up for the FREE newsletter AP News--updates, tricks of the trade > and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' > request to APNews@fullstaff.org. Please include your name and > specialty in the body of the email. > > --- On Mon, 2/4/13, histonet-request@lists.utsouthwestern.edu < > histonet-request@lists.utsouthwestern.edu> wrote: _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HParker <@t> Skaggs.Net Tue Feb 5 14:48:14 2013 From: HParker <@t> Skaggs.Net (Parker, Helayne) Date: Tue Feb 5 14:48:19 2013 Subject: [Histonet] Bronch Order Forms In-Reply-To: References: Message-ID: <930EB2E8DF68C544873EDD2A3D5F506007D0300CF0@email1.skaggs.net> Hi All, Not sure how many of you do cytologies in your departments? We do quite a bit of bronchs around here and about 5 years ago a new physician who is no longer here developed the form that we currently use. It has never been liked by the nurses in scopes etc and it is pretty complicated. It allows Drs to put up to 5 microbiology, hematology, cyto or histo samples (many of which we share in all depts) on one form and has multiple check boxes to check as to what tests they want for each sample in multiple columns. We are looking to streamline this form to hopefully make it easier for everyone involved. Any of you have any Bronch ordering forms you really like or have used some in the past. Needs to be a paper order as we are not on a Lab system in Path at this point. Thanks, Helayne Parker, H.T. (ASCP) Pathology Section Head Cox Medical Center Branson P.O. Box 650, Branson, MO 65615 Phone: 417-335-7254 Fax: 417-335-7127 Email: hparker@skaggs.net Web: www.coxhealth.com/branson -------------- next part -------------- CoxHealth ? ranked one of Missouri's Best Hospitals by U.S. News & World Report COXHEALTH CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From rjbuesa <@t> yahoo.com Tue Feb 5 14:49:40 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Feb 5 14:49:43 2013 Subject: [Histonet] HER-2 In-Reply-To: References: <1359773541.80743.YahooMailNeo@web125406.mail.ne1.yahoo.com> Message-ID: <1360097380.79198.YahooMailNeo@web163105.mail.bf1.yahoo.com> Yes, that is sometimes a common occurrence amongst pathologists, BUT those differences have to be solved in conference before issuing the report. Difficult cases (at least at my hospital) are reviewed in conference, I agree with you: your protocol (specially if it is based on DAKO's protocol) should remain as is. The diagnosis differences should not determine a change. Ren? J. From: Mark Tarango To: Wilson A Cc: "histonet@lists.utsouthwestern.edu" Sent: Tuesday, February 5, 2013 2:24 PM Subject: Re: [Histonet] HER-2 I'd be interested to know if all your pathologists agree that the 2+ cases are 2+.? Is it possible that one of the pathologists is calling more cases as 2+ than the rest?? I would have a lot of questions before modifying the staining protocol.? It would be helpful you posted more info. Mark On Fri, Feb 1, 2013 at 6:52 PM, Wilson A wrote: > >? ? Hi, >? ? ? Our pathologists are concerned we may be reporting too many 2+ > HER2?s.? Can someone? help with this? > >? ? ? Thanks, >? ? Wilson > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sdysart <@t> mirnarx.com Tue Feb 5 14:59:40 2013 From: sdysart <@t> mirnarx.com (Sarah Dysart) Date: Tue Feb 5 14:59:49 2013 Subject: [Histonet] PRN pay? Message-ID: <8A70A9B2ECDD084DACFE6C59FCF86D50484FD72C@BL2PRD0711MB434.namprd07.prod.outlook.com> How much is the standard pay for PRN in Texas? IHC, specials, and routine to be included in the work. Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 From kiran_g <@t> sbcglobal.net Tue Feb 5 15:19:07 2013 From: kiran_g <@t> sbcglobal.net (Kiranjit Grewal) Date: Tue Feb 5 15:19:10 2013 Subject: [Histonet] Prisma H&E Stainer Throughput Message-ID: <1360099147.60532.YahooMailClassic@web184405.mail.bf1.yahoo.com> Hello Histonetters, ? I am looking for a number for throughput on Prisma stainer in your Lab. we use ovens on the stainer for baking the slides. Our throughput is less than the manufactures throughput. ? Any input is appreciated. ? thank you in advance, ? -Kiran From marktarango <@t> gmail.com Tue Feb 5 15:32:45 2013 From: marktarango <@t> gmail.com (Mark Tarango) Date: Tue Feb 5 15:32:49 2013 Subject: [Histonet] HER-2 In-Reply-To: <1360097380.79198.YahooMailNeo@web163105.mail.bf1.yahoo.com> References: <1359773541.80743.YahooMailNeo@web125406.mail.ne1.yahoo.com> <1360097380.79198.YahooMailNeo@web163105.mail.bf1.yahoo.com> Message-ID: Many pathologists, if they have any doubt about the score will just say that it is 2+ so that its gets HER2 by FISH which is considered the best method for determining HER2 status. Even if by the scoring criteria it is a 1+ but the intensity is a little stronger than normal (but maybe basolateral or not complete staining) they just go with 2+. Sometimes its high grade 1+ and it doesn't quite meet the 2+ staining criteria and they call it 2+ too. If these things are happening enough it could mean calling more 2+ cases. They don't always follow the scoring criteria to the letter. We use digital image analysis for HER2 IHC scoring and the computer is pretty right on (matches with FISH), but sometimes the pathologist will change the score in the report to 2+ even though it's a 1+ by the computer. There is one pathologist in particular who doesn't believe in the computer reading the HER2 score and is trying very hard to find cases that are positive by FISH but that the computer is calling the IHC 1+. He also requests FISH on some 3+ cases to try and find any over-calling by the computer. The thought of a woman getting a toxic drug needlessly really bothers him. So without Wilson posting more info there's not much help that I think anyone can offer. This is stain that has to be validated more extensively than others, so the protocol shouldn't just be tweaked. How do the controls look? Was there any lot to lot variation? Lots of questions.. Mark On Tue, Feb 5, 2013 at 12:49 PM, Rene J Buesa wrote: > Yes, that is sometimes a common occurrence amongst pathologists, BUT those > differences have to be solved in conference before issuing the report. > Difficult cases (at least at my hospital) are reviewed in conference, > I agree with you: your protocol (specially if it is based on DAKO'sprotocol) should remain as is. > The diagnosis differences should not determine a change. > Ren? J. > > *From:* Mark Tarango > *To:* Wilson A > *Cc:* "histonet@lists.utsouthwestern.edu" < > histonet@lists.utsouthwestern.edu> > *Sent:* Tuesday, February 5, 2013 2:24 PM > *Subject:* Re: [Histonet] HER-2 > > I'd be interested to know if all your pathologists agree that the 2+ cases > are 2+. Is it possible that one of the pathologists is calling more cases > as 2+ than the rest? I would have a lot of questions before modifying the > staining protocol. It would be helpful you posted more info. > > Mark T. > > On Fri, Feb 1, 2013 at 6:52 PM, Wilson A wrote: > > > > > Hi, > > Our pathologists are concerned we may be reporting too many 2+ > > HER2?s. Can someone help with this? > > > > Thanks, > > Wilson > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From Joyce.Weems <@t> emoryhealthcare.org Tue Feb 5 15:39:17 2013 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Tue Feb 5 15:39:26 2013 Subject: [Histonet] HER-2 In-Reply-To: <1359773541.80743.YahooMailNeo@web125406.mail.ne1.yahoo.com> References: <1359773541.80743.YahooMailNeo@web125406.mail.ne1.yahoo.com> Message-ID: I would suggest that you send several of your own cases to a reference lab that reads tons of them and see what numbers they report. Then compare the two results. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph?s Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Wilson A Sent: Friday, February 01, 2013 9:52 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HER-2 Hi, Our pathologists are concerned we may be reporting too many 2+ HER2?s. Can someone help with this? Thanks, Wilson _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From rennie1108 <@t> yahoo.com Tue Feb 5 15:58:26 2013 From: rennie1108 <@t> yahoo.com (Adrienne Anderson) Date: Tue Feb 5 15:58:29 2013 Subject: [Histonet] Xylene Substitutes Message-ID: <1360101506.39015.YahooMailNeo@web125606.mail.ne1.yahoo.com> Hello all, My lab is looking into xylene substitutes, and I'd love some feedback on what other labs are using. We currently use SubX, but are there other items out there more economical? Thanks, Adrienne From Wanda.Smith <@t> HCAhealthcare.com Tue Feb 5 16:10:41 2013 From: Wanda.Smith <@t> HCAhealthcare.com (Wanda.Smith@HCAhealthcare.com) Date: Tue Feb 5 16:10:46 2013 Subject: [Histonet] Cytology Question for you Cytotechs Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BA27D1C04832@NADCWPMSGCMS03.hca.corpad.net> Or a question for you HT and HTL's that manage Cytotechs in your labs.... Is it written ANYWHERE that a Cytotech cannot or should not review their correlations and make the call whether there is a discrepancy or miss??? Then document themselves on it? Does this make sense? Thanks, Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. From Jessica.Vacca <@t> HCAhealthcare.com Tue Feb 5 17:33:26 2013 From: Jessica.Vacca <@t> HCAhealthcare.com (Jessica.Vacca@HCAhealthcare.com) Date: Tue Feb 5 17:33:31 2013 Subject: [Histonet] Frozen Section Charges Message-ID: <938D716CD445614ABBB817517557B6F407CCDF20E9@NADCWPMSGCMS09.hca.corpad.net> Can Frozens (88331 or 88332) be charged at a Technical AND Professional level or Professional only? Thanks From tkngflght <@t> yahoo.com Tue Feb 5 21:02:52 2013 From: tkngflght <@t> yahoo.com (Cheryl) Date: Tue Feb 5 21:02:59 2013 Subject: [Histonet] Joe Nocito's request -- oh my! In-Reply-To: <5A2BD13465E061429D6455C8D6B40E3916490A9A97@IBMB7Exchange.digestivespecialists.com> Message-ID: <1360119772.5144.YahooMailClassic@web161905.mail.bf1.yahoo.com> OMGoodness.? I am so sorry-- ? Did it from my phone...thought I'd cleaned it but didn't scroll to look. ? Reposting the whole thing is one of my peeves, too! Again, my apologies.? I'll probably do it again some day so in advance--SO SORRY!! ? xoxo ? Cheryl ? From tkngflght <@t> yahoo.com Tue Feb 5 21:07:09 2013 From: tkngflght <@t> yahoo.com (Cheryl) Date: Tue Feb 5 21:07:11 2013 Subject: [Histonet] ER/PR and Her2 image analysis Message-ID: <1360120029.58613.YahooMailClassic@web161902.mail.bf1.yahoo.com> Hey- Help!? ? Will ya'll share what image analysis software/hardware you're using?? Is anyone doing full digital quatifying analysis of ER, PR and Her2 ?? ? ? Thanks! Cheryl Kerry, HT(ASCP), AP Manager Pathology Group of Louisiana ckerry@pathgroupla.com ? (LOOK GUYS!!? No digest :) ) From TMcNemar <@t> lmhealth.org Wed Feb 6 04:36:38 2013 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Wed Feb 6 04:36:59 2013 Subject: [Histonet] Xylene Substitutes In-Reply-To: <1360101506.39015.YahooMailNeo@web125606.mail.ne1.yahoo.com> References: <1360101506.39015.YahooMailNeo@web125606.mail.ne1.yahoo.com> Message-ID: Not familiar with SubX but we have used Americlear for many years with good results. You just can't beat Xylene for some things though and still keep a little around. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Adrienne Anderson Sent: Tuesday, February 05, 2013 4:58 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Xylene Substitutes Hello all, My lab is looking into xylene substitutes, and I'd love some feedback on what other labs are using. We currently use SubX, but are there other items out there more economical? Thanks, Adrienne _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From Joyce.Weems <@t> emoryhealthcare.org Wed Feb 6 05:07:40 2013 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Wed Feb 6 05:07:49 2013 Subject: [Histonet] RE: Frozen Section Charges In-Reply-To: <938D716CD445614ABBB817517557B6F407CCDF20E9@NADCWPMSGCMS09.hca.corpad.net> References: <938D716CD445614ABBB817517557B6F407CCDF20E9@NADCWPMSGCMS09.hca.corpad.net> Message-ID: Both Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jessica.Vacca@HCAhealthcare.com Sent: Tuesday, February 05, 2013 6:33 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Frozen Section Charges Can Frozens (88331 or 88332) be charged at a Technical AND Professional level or Professional only? Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From irena.kirbis <@t> hotmail.com Wed Feb 6 07:02:58 2013 From: irena.kirbis <@t> hotmail.com (IRENA SREBOTNIK KIRBIS) Date: Wed Feb 6 07:03:02 2013 Subject: [Histonet] cryostat Message-ID: Hi,I wonder what is the way of removing shavings/trimmings from the cryostat in other lab?, with the wet paper? gauze?, household vacuum cleaner - yes I saw this in one lab!?thanksIrena From rennie1108 <@t> yahoo.com Wed Feb 6 07:30:29 2013 From: rennie1108 <@t> yahoo.com (Adrienne Anderson) Date: Wed Feb 6 07:30:38 2013 Subject: [Histonet] Xylene Substitutes In-Reply-To: References: <1360101506.39015.YahooMailNeo@web125606.mail.ne1.yahoo.com> Message-ID: <811CA4FC-4652-43F8-ACC8-80D9DE1AE3B2@yahoo.com> Thanks so much, everyone, for the feedback. I have a colleague who is convinced paint thinner is exactly the same as the SubX we use. Does anyone know if this is true? Thanks again, Adrienne On Feb 6, 2013, at 5:36 AM, Tom McNemar wrote: > Not familiar with SubX but we have used Americlear for many years with good results. You just can't beat Xylene for some things though and still keep a little around. > > Tom McNemar, HT(ASCP) > Histology Co-ordinator > Licking Memorial Health Systems > (740) 348-4163 > (740) 348-4166 > tmcnemar@lmhealth.org > www.LMHealth.org > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Adrienne Anderson > Sent: Tuesday, February 05, 2013 4:58 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Xylene Substitutes > > Hello all, > > My lab is looking into xylene substitutes, and I'd love some feedback on what other labs are using. We currently use SubX, but are there other items out there more economical? > > Thanks, > Adrienne > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From Janine.SimmsColon <@t> jmmc.com Wed Feb 6 07:32:10 2013 From: Janine.SimmsColon <@t> jmmc.com (SimmsColon, Janine) Date: Wed Feb 6 07:32:15 2013 Subject: [Histonet] Xylene Substitutes In-Reply-To: <1360101506.39015.YahooMailNeo@web125606.mail.ne1.yahoo.com> References: <1360101506.39015.YahooMailNeo@web125606.mail.ne1.yahoo.com> Message-ID: We use Pro Par Clearant through Fisher from Anatech. Health-1, Flammablity-2, Instability-0. For a case of 4 gallons it runs us about $170. Janine Simms Colon, CPhT, HT(ASCP), BHA Histology/Pathology Johnson Memorial Hospital 201 Chestnut Hill Road Stafford Springs, CT 06076 Office: 860-684-8230 ext. 5197 janine.simmscolon@jmmc.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Adrienne Anderson Sent: Tuesday, February 05, 2013 4:58 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Xylene Substitutes Hello all, My lab is looking into xylene substitutes, and I'd love some feedback on what other labs are using. We currently use SubX, but are there other items out there more economical? Thanks, Adrienne _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. From joewalker <@t> rrmc.org Wed Feb 6 07:40:52 2013 From: joewalker <@t> rrmc.org (Joe W. Walker, Jr.) Date: Wed Feb 6 07:40:56 2013 Subject: [Histonet] RE: Cytology Question for you Cytotechs In-Reply-To: <9E2D36CE2D7CBA4A94D9B22E8328A3BA27D1C04832@NADCWPMSGCMS03.hca.corpad.net> References: <9E2D36CE2D7CBA4A94D9B22E8328A3BA27D1C04832@NADCWPMSGCMS03.hca.corpad.net> Message-ID: <3C2378778400AD448ADA6FD6BDB7CCCC179DEAB3@RRMBX03.rrmc.local> Good morning, Wanda, The answer is "it depends". CLIA is pretty specific on what a cytotechs can and cannot do. If your cytotechnologist qualifies as a cytology general supervisor, then they could perform the function that you are referring. CLIA 493.1469, which refers to cytology general supervisors states: ...(a) Be qualified as a technical supervisor under ? 493.1449 (b) or (k); or (b)(1) Be qualified as a cytotechnologist under ? 493.1483; and (2) Have at least 3 years of full-time (2,080 hours per year) experience as a cytotechnologist within the preceding 10 years... As to whether the cytotech should or should perform the task, this should be discussed with your technical supervisor of the laboratory who is ultimately responsible for the quality of work that occurs within your laboratory. I personally believe that for an effective quality management program in cytology, the review you are describing should be performed by a different individual. Cytotechnologists should be allowed to review the cases that are determined to be a miss and should also have input into what is considered "a miss" within your laboratory. If you would like to contact me off line, I have lots of material that I could share with you concerning best practices of quality management in the cytology laboratory. Cheers, Joe W. Walker, Jr. MS, SCT(ASCP)CM Anatomical Pathology Manager Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P: 802.747.1790 F: 802.747.6525 NEW EMAIL: joewalker@rrmc.org www.rrmc.org Our Vision: To be the Best Community Healthcare System in New England Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet Recognition? and the Governor's Award for Performance Excellence -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Wanda.Smith@HCAhealthcare.com Sent: Tuesday, February 05, 2013 5:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cytology Question for you Cytotechs Or a question for you HT and HTL's that manage Cytotechs in your labs.... Is it written ANYWHERE that a Cytotech cannot or should not review their correlations and make the call whether there is a discrepancy or miss??? Then document themselves on it? Does this make sense? Thanks, Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. Thank You From vavalos <@t> allergydermatology.com Wed Feb 6 08:06:38 2013 From: vavalos <@t> allergydermatology.com (Vanessa Avalos) Date: Wed Feb 6 08:06:50 2013 Subject: [Histonet] Xylene Substitutes In-Reply-To: <811CA4FC-4652-43F8-ACC8-80D9DE1AE3B2@yahoo.com> References: <1360101506.39015.YahooMailNeo@web125606.mail.ne1.yahoo.com> <811CA4FC-4652-43F8-ACC8-80D9DE1AE3B2@yahoo.com> Message-ID: I use XS-3 from Statlab for staining. Since it is substitute I had to do some adjusting to the stain line but once I got it right I have had no problems We pay about $70 for 4 gal. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Adrienne Anderson Sent: Wednesday, February 06, 2013 6:30 AM To: Tom McNemar Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Xylene Substitutes Thanks so much, everyone, for the feedback. I have a colleague who is convinced paint thinner is exactly the same as the SubX we use. Does anyone know if this is true? Thanks again, Adrienne On Feb 6, 2013, at 5:36 AM, Tom McNemar wrote: > Not familiar with SubX but we have used Americlear for many years with good results. You just can't beat Xylene for some things though and still keep a little around. > > Tom McNemar, HT(ASCP) > Histology Co-ordinator > Licking Memorial Health Systems > (740) 348-4163 > (740) 348-4166 > tmcnemar@lmhealth.org > www.LMHealth.org > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Adrienne Anderson > Sent: Tuesday, February 05, 2013 4:58 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Xylene Substitutes > > Hello all, > > My lab is looking into xylene substitutes, and I'd love some feedback on what other labs are using. We currently use SubX, but are there other items out there more economical? > > Thanks, > Adrienne > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> gmail.com Wed Feb 6 09:03:32 2013 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Wed Feb 6 09:03:37 2013 Subject: [Histonet] Re: HER-2 Message-ID: Mark Tarango notes: >>Many pathologists, if they have any doubt about the score will just say that it is 2+ so that its gets HER2 by FISH which is considered the best method for determining HER2 status.<< On one busy pathology service I worked in 2004-2006 we were quite explicit about overcalling HER-2 by IHC 2+. If we had any doubts at all, we sent out the FISH. The cost is trivial compared to the cost of treating a woman with trastuzumab (Herceptin). It's important to understand that IHC and FISH do not measure the same thing - IHC is looking at the excessive amount of the gene product, while FISH is looking at amplification of the gene itself. Neither is necessarily the "best method". Some oncologists want both methods done on all cases. PCR further complicates the situation. I think some reference labs now consider this the preferred HER-2 method for adenocarcinomas of the stomach and esophagus. Bob Richmond Samurai Pathologist Maryville TN From rjbuesa <@t> yahoo.com Wed Feb 6 09:16:39 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Feb 6 09:16:43 2013 Subject: [Histonet] Xylene Substitutes In-Reply-To: References: <1360101506.39015.YahooMailNeo@web125606.mail.ne1.yahoo.com> Message-ID: <1360163799.83270.YahooMailNeo@web163106.mail.bf1.yahoo.com> The best solution to eliminate xylene is to use isopropyl alcohol mixed with mineral oil. Xylene can be eliminated from staining by dewaxing with 2% aq. sol. of dishwasher soap. Before coverslipping oven dry the stained sections and cover directly. To clean tissue processors use a 2% mixture of strong lab lab-ware detergent. Do all of the above and your lab will be xylene free. Ren? J. From: Tom McNemar To: 'Adrienne Anderson' ; "histonet@lists.utsouthwestern.edu" Sent: Wednesday, February 6, 2013 5:36 AM Subject: RE: [Histonet] Xylene Substitutes Not familiar with SubX but we have used Americlear for many years with good results.? You just can't beat Xylene for some things though and still keep a little around. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org http://www.lmhealth.org/ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Adrienne Anderson Sent: Tuesday, February 05, 2013 4:58 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Xylene Substitutes Hello all, My lab is looking into xylene substitutes, and I'd love some feedback on what other labs are using. We currently use SubX, but are there other items out there more economical? Thanks, Adrienne _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> gmail.com Wed Feb 6 09:16:46 2013 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Wed Feb 6 09:16:51 2013 Subject: [Histonet] Re: Xylene Substitutes Message-ID: Adrienne Anderson (where?) asks: >>My lab is looking into xylene substitutes, and I'd love some feedback on what other labs are using. We currently use SubX, but are there other items out there more economical?<< I never heard of SubX, but the Leica Microsystems Richmond [no kin!] Inc. MSDS describes it as an "Aliphatic hydrocarbon, isoparaffinic oil", of which there are a great number in the trade. The flash point is 106 degrees Fahrenheit (xylene is 78, some others as high as 104). These aliphatics are not compatible with each other if you're going to recover them by distillation, and with any of them you need to sit down with management and make clear that you're going to go with a particular trade name and that they can't substitute it with naphtha from a ma-and-pa repackaging operation. AmeriClear of course is limonene, a turpentine-like substance distilled from citrus peels. Many people find the citrus smell intolerable, or are allergic to it, and I think the limonenes (there are several other brands) are no longer in widespread use, particularly since the price has gone up. Please, folks, when you post trade names on HistoNet, take a moment to look at the MSDS (it's online) to see what the chemical identity of the trade-name product is. You should know this if you're using the product, and it's better to assume that other people don't know it. Bob Richmond Samurai Pathologist Maryville TN From rjbuesa <@t> yahoo.com Wed Feb 6 09:19:16 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Feb 6 09:19:21 2013 Subject: [Histonet] Xylene Substitutes In-Reply-To: <811CA4FC-4652-43F8-ACC8-80D9DE1AE3B2@yahoo.com> References: <1360101506.39015.YahooMailNeo@web125606.mail.ne1.yahoo.com> <811CA4FC-4652-43F8-ACC8-80D9DE1AE3B2@yahoo.com> Message-ID: <1360163956.69159.YahooMailNeo@web163103.mail.bf1.yahoo.com> Paint thinners?are somatimes?as dangerous as xylene is?and less effective. If you want additional information about eliminating xylene please read my articles at www.histosearch.com/rene.html ? Ren? J. From: Adrienne Anderson To: Tom McNemar Cc: "histonet@lists.utsouthwestern.edu" Sent: Wednesday, February 6, 2013 8:30 AM Subject: Re: [Histonet] Xylene Substitutes Thanks so much, everyone, for the feedback. I have a colleague who is convinced paint thinner is exactly the same as the SubX we use. Does anyone know if this is true? Thanks again, Adrienne On Feb 6, 2013, at 5:36 AM, Tom McNemar wrote: > Not familiar with SubX but we have used Americlear for many years with good results.? You just can't beat Xylene for some things though and still keep a little around. > > Tom McNemar, HT(ASCP) > Histology Co-ordinator > Licking Memorial Health Systems > (740) 348-4163 > (740) 348-4166 > tmcnemar@lmhealth.org > http://www.lmhealth.org/ > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Adrienne Anderson > Sent: Tuesday, February 05, 2013 4:58 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Xylene Substitutes > > Hello all, > > My lab is looking into xylene substitutes, and I'd love some feedback on what other labs are using. We currently use SubX, but are there other items out there more economical? > > Thanks, > Adrienne > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Nancy_Schmitt <@t> pa-ucl.com Wed Feb 6 09:42:24 2013 From: Nancy_Schmitt <@t> pa-ucl.com (Nancy Schmitt) Date: Wed Feb 6 09:42:38 2013 Subject: [Histonet] fixation Message-ID: <906B4DA90ED1DB4DB6C7E94D7CEE6C364DC6BD62@PEITHA.wad.pa-ucl.com> Good Morning- Scenario: a specimen is received and has been in cytology fixative overnight - a cell block is made from (very small) tissue picked out. 1. Can this be run on a program without formalin? 2. Does this need to be in formalin before going on the processor - if so how long? I appreciate any thoughts you may have, Nancy NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From rjbuesa <@t> yahoo.com Wed Feb 6 09:45:02 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Feb 6 09:45:05 2013 Subject: [Histonet] fixation In-Reply-To: <906B4DA90ED1DB4DB6C7E94D7CEE6C364DC6BD62@PEITHA.wad.pa-ucl.com> References: <906B4DA90ED1DB4DB6C7E94D7CEE6C364DC6BD62@PEITHA.wad.pa-ucl.com> Message-ID: <1360165502.23771.YahooMailNeo@web163106.mail.bf1.yahoo.com> If you already have the cell block you can put it in the tissue processor. You do not need to re-fix it, but it will when going through the formalin stations in the tissue processor, and that OK and enough. Ren? J. From: Nancy Schmitt To: "histonet@lists.utsouthwestern.edu" Sent: Wednesday, February 6, 2013 10:42 AM Subject: [Histonet] fixation Good Morning- Scenario:? a specimen is received and has been in cytology fixative overnight - a cell block is made from (very small) tissue picked out. 1.? ? ? ? Can this be run on a program without formalin? 2.? ? ? Does this need to be in formalin before going on the processor - if so how long? I appreciate any thoughts you may have, Nancy NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From twebster <@t> CRH.org Wed Feb 6 10:15:52 2013 From: twebster <@t> CRH.org (Webster, Thomas S.) Date: Wed Feb 6 10:16:10 2013 Subject: [Histonet] Cytology Question for you Cytotechs Message-ID: <7207186ED68FB542803CAF1CE6E82FF80499FD@exmb1.crh.org> We always have a different person make the review. In our situation, the pathologist makes those decisions on whether it was miss. CONFIDENTIALITY NOTICE: This e-mail message, including all attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. You may NOT use, disclose, copy or disseminate this information. If you are not the intended recipient, please contact the sender by reply e-mail immediately. Please destroy all copies of the original message and all attachments. Your cooperation is greatly appreciated. Columbus Regional Hospital 2400 East 17th Street Columbus, Indiana 47201 From marktarango <@t> gmail.com Wed Feb 6 10:20:13 2013 From: marktarango <@t> gmail.com (Mark Tarango) Date: Wed Feb 6 10:20:36 2013 Subject: [Histonet] ER/PR and Her2 image analysis In-Reply-To: <1360120029.58613.YahooMailClassic@web161902.mail.bf1.yahoo.com> References: <1360120029.58613.YahooMailClassic@web161902.mail.bf1.yahoo.com> Message-ID: Hi Cheryl, We use the Aperio system for HER2 scoring. Our lab manager put a cytotech in charge of validating the digital reading and her next project is ER and PR. For HER2 IHC, the software is initially set for Dako's Hercept test. We don't use Dako, we use Ventana staining platform and the 4B5 clone for HER2 IHC. She had to play with the software until it was scoring cases appropriately, but it works great now. The initial slide scanner we got from them was a lemon. They sent another and it was a lemon too. The third instrument (which is a newer model) usually works without any problems. The system helps with FISH too. The pathologist can mark the area of interest for FISH on the digital slide image. This is great for my lab because we have pathologists that are not here at our main lab site. This allows me to mark the area and start FISH even though the IHC or H&E might be off-site. Mark T. On Tue, Feb 5, 2013 at 7:07 PM, Cheryl wrote: > Hey- Help! > > Will ya'll share what image analysis software/hardware you're using? Is > anyone doing full digital quatifying analysis of ER, PR and Her2 ? > > > Thanks! > > > Cheryl Kerry, HT(ASCP), AP Manager > Pathology Group of Louisiana > ckerry@pathgroupla.com > > (LOOK GUYS!! No digest :) ) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From abright <@t> brightinstruments.com Wed Feb 6 10:31:04 2013 From: abright <@t> brightinstruments.com (Alan Bright) Date: Wed Feb 6 10:31:30 2013 Subject: [Histonet] cryostat In-Reply-To: References: Message-ID: I would not vacuum the trimmings out of cryostat, if it were infected It would make a good job of contaminating a laboratory etc. Terrified Alan Bright Sent from my iPhone > > > > > Hi,I wonder what is the way of removing shavings/trimmings from the cryostat in other lab?, with the wet paper? gauze?, household vacuum cleaner - yes I saw this in one lab!?thanksIrena _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > From marktarango <@t> gmail.com Wed Feb 6 10:36:54 2013 From: marktarango <@t> gmail.com (Mark Tarango) Date: Wed Feb 6 10:36:58 2013 Subject: [Histonet] Re: HER-2 In-Reply-To: References: Message-ID: HER2 FISH really is considered the best method. It's the only method that has actually been tied directly to patient outcome. The other methods are expert consensus based. That being said, there are some cases that are equivocal by FISH. Sometimes we have to ignore the CEP-17 (green) signals and report the HER2 status based only the HER2 copy number. If the HER2/CEP17 ratio is equivocal but the average HER2 count is over 6, we add a comment explaining that it can be considered positive. If it's between 4-6, we say it's equivocal and usually suggest repeating on the excision. If it's under 4, we say that it can be considered to be negative. We can resolve most cases this way. In rare instances we have sent out to another lab to perform alternative FISH probes to enumerate for chromosome 17 (probes SMS/RARA to Phenopath). We have not found this to be especially useful. If a case is 3+ by IHC but for some reason the oncologist wants FISH to confirm, the patient will not be treated if FISH is negative. There could be other reasons besides gene amplification for accumulation or expression of the HER2 protein. The drug is only proven to work when the gene is amplified and you test that by FISH. PCR has it's own problems too. The biggest thing, I think, is that you dilute your HER2 score by including normal tissue during macro or micro-dissection. This does not work well for small foci of tumor. Many stomach and esophagus biopsies contain only small amounts of tumor. Mark On Wed, Feb 6, 2013 at 7:03 AM, Bob Richmond wrote: > Mark Tarango notes: > > >>Many pathologists, if they have any doubt about the score will just say > that it is 2+ so that its gets HER2 by FISH which is considered the best > method for determining HER2 status.<< > > On one busy pathology service I worked in 2004-2006 we were quite > explicit about overcalling HER-2 by IHC 2+. If we had any doubts at > all, we sent out the FISH. The cost is trivial compared to the cost of > treating a woman with trastuzumab (Herceptin). > > It's important to understand that IHC and FISH do not measure the same > thing - IHC is looking at the excessive amount of the gene product, > while FISH is looking at amplification of the gene itself. Neither is > necessarily the "best method". Some oncologists want both methods done > on all cases. > > PCR further complicates the situation. I think some reference labs now > consider this the preferred HER-2 method for adenocarcinomas of the > stomach and esophagus. > > Bob Richmond > Samurai Pathologist > Maryville TN > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From liz <@t> premierlab.com Wed Feb 6 10:40:33 2013 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Wed Feb 6 10:43:26 2013 Subject: [Histonet] cryostat In-Reply-To: References: , Message-ID: <14E2C6176416974295479C64A11CB9AE016411AB4629@SBS2K8.premierlab.local> Happy Wednesday Everyone There are vacumes specifically made for cryostats that are hepa filtered, etc. We have one of those. The company is called MARMED. We use a shop vac for the paraffin microtomes, that works nicely. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 881-0763 cell (303) 682-9060 fax liz@premierlab.com Ship to address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Alan Bright [abright@brightinstruments.com] Sent: Wednesday, February 06, 2013 9:31 AM To: IRENA SREBOTNIK KIRBIS Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] cryostat I would not vacuum the trimmings out of cryostat, if it were infected It would make a good job of contaminating a laboratory etc. Terrified Alan Bright Sent from my iPhone > > > > > Hi,I wonder what is the way of removing shavings/trimmings from the cryostat in other lab?, with the wet paper? gauze?, household vacuum cleaner - yes I saw this in one lab!?thanksIrena _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mjdessoye <@t> commonwealthhealth.net Wed Feb 6 10:46:53 2013 From: mjdessoye <@t> commonwealthhealth.net (Dessoye, Michael J) Date: Wed Feb 6 10:47:00 2013 Subject: [Histonet] Xylene Substitutes In-Reply-To: <1360101506.39015.YahooMailNeo@web125606.mail.ne1.yahoo.com> Message-ID: We used SubX but have switched to Clear-Rite 3 from Thermo Fisher with excellent results as well as cost savings. Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdessoye@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 -----Original Message----- From: Adrienne Anderson [mailto:rennie1108@yahoo.com] Sent: Tuesday, February 05, 2013 4:58 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Xylene Substitutes Hello all, My lab is looking into xylene substitutes, and I'd love some feedback on what other labs are using. We currently use SubX, but are there other items out there more economical? Thanks, Adrienne _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Commonwealth Health. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. From baustin <@t> cbgbiotech.com Wed Feb 6 10:50:25 2013 From: baustin <@t> cbgbiotech.com (Beth Austin-Sell) Date: Wed Feb 6 10:50:28 2013 Subject: [Histonet] Xylene Substitutes Message-ID: Adrienne, If you'd like information on Formula 83 please contact me off list at beth@cbgbiotech.com Thanks, Beth Sell CBG From lpjones <@t> srhs-pa.org Wed Feb 6 10:59:40 2013 From: lpjones <@t> srhs-pa.org (Jones, Laura) Date: Wed Feb 6 11:03:49 2013 Subject: [Histonet] cryostat In-Reply-To: References: Message-ID: <4AE8039AEA096143B965CBC6D092166802351B39EF@EXCH2007.srhs-pa.org> Hi Irena! If you go to IMEB Inc.'s website, and type "vacuum" into the search, it will take you to what we use. They sell a vacuum cleaner and accessories. We currently use the filtered hoses with an old canister vacuum that's been around longer than I have. When the filter gets full, we discard it in the biohazard trash and replace it with a new one. Hope this helps! Laura ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of IRENA SREBOTNIK KIRBIS [irena.kirbis@hotmail.com] Sent: Wednesday, February 06, 2013 8:02 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cryostat Hi,I wonder what is the way of removing shavings/trimmings from the cryostat in other lab?, with the wet paper? gauze?, household vacuum cleaner - yes I saw this in one lab!?thanksIrena _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sharon Regional Health System is the area's largest hospital and provider of health care services. Visit us online at http://www.sharonregional.com for a complete listing of our services, primary care physicians and specialists, and satellite locations. Confidentiality Note: This message is intended for use only by the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. Thank you. From katemaddox <@t> yahoo.com Wed Feb 6 11:06:34 2013 From: katemaddox <@t> yahoo.com (Kathryn Maddox) Date: Wed Feb 6 11:06:38 2013 Subject: [Histonet] P16 on Leica Bond Message-ID: <1360170394.17396.YahooMailNeo@web163404.mail.gq1.yahoo.com> If anyone out there is performing? p16 on the Leica Bond immunostainer, could you please tell me where you purchase your antibody and what protocol you are using? Thanks so much! Kathy Maddox HT{ASCP} Lake Charles, Louisiana From lblazek <@t> digestivespecialists.com Wed Feb 6 11:13:40 2013 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Wed Feb 6 11:14:29 2013 Subject: [Histonet] Xylene Substitutes In-Reply-To: References: <1360101506.39015.YahooMailNeo@web125606.mail.ne1.yahoo.com> Message-ID: <5A2BD13465E061429D6455C8D6B40E3916490A9AAA@IBMB7Exchange.digestivespecialists.com> We have used Formula 83 from CBG for years with excellent results. We use it for processing and staining. We also recycle it making the cost very low. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dessoye, Michael J Sent: Wednesday, February 06, 2013 11:47 AM To: Adrienne Anderson; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Xylene Substitutes We used SubX but have switched to Clear-Rite 3 from Thermo Fisher with excellent results as well as cost savings. Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdessoye@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 -----Original Message----- From: Adrienne Anderson [mailto:rennie1108@yahoo.com] Sent: Tuesday, February 05, 2013 4:58 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Xylene Substitutes Hello all, My lab is looking into xylene substitutes, and I'd love some feedback on what other labs are using. We currently use SubX, but are there other items out there more economical? Thanks, Adrienne _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Commonwealth Health. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mgiorgi <@t> icplab.com Wed Feb 6 11:18:22 2013 From: mgiorgi <@t> icplab.com (Miranda Giorgi) Date: Wed Feb 6 11:18:27 2013 Subject: [Histonet] Graveyard Histotech Position in Spokane, Washington Message-ID: <46BB82E6FC36E44FB454E00C82DBDC56CD1A80@icpmail.PAI.E-PATHOLOGY.com> Hello All, Please see the job posting below for a Histotech position at InCyte Pathology in Spokane, WA. Only interested candidates should reply. Please do not respond if you are from a recruitment service. Thank you! Histology Analyst, Graveyard Shift (M-F) InCyte Pathology is a full-service, pathologist-owned anatomic pathology laboratory. Our one-hundred employees and twenty-one board-certified pathologists provide exceptional laboratory services to hospitals and physician offices throughout the Pacific Northwest, Montana, and Alaska from our state-of-the-art facility located in Spokane Valley, Washington. We are just a short drive from five major ski resorts, several beautiful lakes, and a variety of outdoor activities. Qualifications: HT certified or HTL registry eligible, and two years of laboratory experience. This position performs all routine procedures in orienting, sectioning, and staining tissue specimens to produce slides which will aide pathologists in diagnosis. Preference will be given to those candidates who are IHC qualified and have proven leadership qualities. We offer competitive salaries and an outstanding benefits package including medical, dental, life and disability insurance, 401(k), and a liberal paid time off program. Relocation assistance is available. Please email your cover letter and resume to humanresources@incytepathology.com. Please reference where you saw this ad. No phone calls or solicitors! Miranda Giorgi, HTL (ASCP)CM Histology Supervisor InCyte Pathology This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the InCyte Privacy Officer at privacy@icplab.com or call (509) 892-2700. From HParker <@t> Skaggs.Net Wed Feb 6 11:49:04 2013 From: HParker <@t> Skaggs.Net (Parker, Helayne) Date: Wed Feb 6 11:49:07 2013 Subject: [Histonet] RE: Histonet Digest, Vol 111, Issue 7 In-Reply-To: <784e0c98-ce64-4f69-8449-72a2cfa059ae@email1.skaggs.net> References: <784e0c98-ce64-4f69-8449-72a2cfa059ae@email1.skaggs.net> Message-ID: <930EB2E8DF68C544873EDD2A3D5F506007D0300CF5@email1.skaggs.net> <<<>>> We use Clear-Rite 3 made by Richard Allan and is purchased at Cardinal. I do not think it is very expensive we buy in a case of 4 gallons. It does a decent job for clearing in the processor and de-paraffinizing slides, but we do coverslip out of xylene at the end of the stain line. Actually we never tried to coverslip out of it Clear-Rite 3 so can not honestly give you a critique in ref to that. We keep both on hand. Xylene for machine cleaning and coverslipping and Clear-Rite 3 for processing and de-paraffinizing. I like it better than Americlear and ProPar. http://www.cardinalhealth.com/us/en/distributedproducts/ASP/C4405-20.asp?cat=physician Helayne Parker, H.T. (ASCP) Pathology Section Head Cox Medical Center Branson P.O. Box 650, Branson, MO 65615 Phone: 417-335-7254 Fax: 417-335-7127 Email: hparker@skaggs.net Web: www.coxhealth.com/branson -------------- next part -------------- CoxHealth ? ranked one of Missouri's Best Hospitals by U.S. News & World Report COXHEALTH CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From cpyse <@t> x-celllab.com Wed Feb 6 12:11:26 2013 From: cpyse <@t> x-celllab.com (Cynthia Pyse) Date: Wed Feb 6 12:11:41 2013 Subject: [Histonet] p16 Message-ID: <003301ce0495$61362660$23a27320$@com> Happy Wednesday Everyone Has anyone been having consistency problems with their p16 staining since MTM has been taken over by Ventana? One day the procedure works another day it doesn't. Same procedure, no variation in the temperature on pretreatment, run on the Dako 48, with no errors. Every other IHC works consistently but the p16, anyone have any thoughts? I do make the DAB fresh daily as suggested in the instructions, it is the same kit that has been running. The only thing I can think of is we do sometimes run the p16 twice a day without making fresh DAB, but the instructions say it can be used within a 24 hour period. This problem is driving me crazy! It just doesn't make any sense. Any help would be appreciated. Thanks in advance. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories 20 Northpointe Parkway Suite 100 Amherst, NY 14228 716-250-9235 etx. 232 e-mail cpyse@x-celllab.com From brett_connolly <@t> merck.com Wed Feb 6 12:15:32 2013 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Wed Feb 6 12:15:36 2013 Subject: [Histonet] CD45 for FFPE mouse tissue Message-ID: Hi all, What's your favorite anti-CD45 for mouse... Serotec rat monoclonal or something else? Thanks, Brett Brett M. Connolly, Ph.D. Principal Scientist, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From Nancy_Schmitt <@t> pa-ucl.com Wed Feb 6 12:19:23 2013 From: Nancy_Schmitt <@t> pa-ucl.com (Nancy Schmitt) Date: Wed Feb 6 12:19:28 2013 Subject: [Histonet] P16 on Leica Bond In-Reply-To: <20130206171231.E9AD71AA036@mail.pa-ucl.com> References: <20130206171231.E9AD71AA036@mail.pa-ucl.com> Message-ID: <906B4DA90ED1DB4DB6C7E94D7CEE6C364DC6CEA3@PEITHA.wad.pa-ucl.com> Kathy- We purchased p16 from Cintec - I believe that was bought out by Ventana. We dilute 1:4 with BOND diluent and run ER2 for 10. Good Luck! Nancy --------------------------------------------- Message: 21 Date: Wed, 6 Feb 2013 09:06:34 -0800 (PST) From: Kathryn Maddox Subject: [Histonet] P16 on Leica Bond To: histonet Message-ID: <1360170394.17396.YahooMailNeo@web163404.mail.gq1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 If anyone out there is performing? p16 on the Leica Bond immunostainer, could you please tell me where you purchase your antibody and what protocol you are using? Thanks so much! Kathy Maddox HT{ASCP} Lake Charles, Louisiana NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From jclark <@t> pcnm.com Wed Feb 6 12:26:04 2013 From: jclark <@t> pcnm.com (Joanne Clark) Date: Wed Feb 6 12:26:14 2013 Subject: [Histonet] RE: Histonet Digest, Vol 111, Issue 7 In-Reply-To: <20130206170342.402678B7036@mx10.myoutlookonline.com> References: <20130206170342.402678B7036@mx10.myoutlookonline.com> Message-ID: <0494A7D4E8CC254EA2FB81464982E378A479B106@S10MAILD001N4.SH10.lan> Hi Kathy, we do p16 on the Leica Bond. You have to purchase the p16 from Ventana, they are the only one who has it. It comes as a predilute and we use it as is with the DAB Refine Detection using retrieval solution ER1 for 10 minutes. Joanne Clark, HT Histology Supervisor Pathology Consultants of New Mexico --------------------------------------------------------------------------------------------------------------------- Message: 21 Date: Wed, 6 Feb 2013 09:06:34 -0800 (PST) From: Kathryn Maddox Subject: [Histonet] P16 on Leica Bond To: histonet Message-ID: <1360170394.17396.YahooMailNeo@web163404.mail.gq1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 If anyone out there is performing? p16 on the Leica Bond immunostainer, could you please tell me where you purchase your antibody and what protocol you are using? Thanks so much! Kathy Maddox HT{ASCP} Lake Charles, Louisiana ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 111, Issue 7 **************************************** From HParker <@t> Skaggs.Net Wed Feb 6 12:27:04 2013 From: HParker <@t> Skaggs.Net (Parker, Helayne) Date: Wed Feb 6 12:27:08 2013 Subject: [Histonet] Xylene/Paint Thinner In-Reply-To: <784e0c98-ce64-4f69-8449-72a2cfa059ae@email1.skaggs.net> References: <784e0c98-ce64-4f69-8449-72a2cfa059ae@email1.skaggs.net> Message-ID: <930EB2E8DF68C544873EDD2A3D5F506007D0300CF6@email1.skaggs.net> <<<>>> Haha- funny you should say that. I always swore that Mitaban (for canine demodex mange) was made out of xylene. Almost looks like watered down xylene. Well I checked the MSDS and it is but only the inactive ingredient part at a high %. On a lighter note that orange fragrance d-limonene flea dip is basically the same stuff as the Americlear is. Helayne Parker, H.T. (ASCP) Pathology Section Head Cox Medical Center Branson P.O. Box 650, Branson, MO 65615 Phone: 417-335-7254 Fax: 417-335-7127 Email: hparker@skaggs.net Web: www.coxhealth.com/branson -------------- next part -------------- CoxHealth ? ranked one of Missouri's Best Hospitals by U.S. News & World Report COXHEALTH CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From cpyse <@t> x-celllab.com Wed Feb 6 12:43:40 2013 From: cpyse <@t> x-celllab.com (Cynthia Pyse) Date: Wed Feb 6 12:43:50 2013 Subject: FW: [Histonet] p16 Message-ID: <004601ce0499$e2710c50$a75324f0$@com> Yes we monitor the temp and humidity. Retrieval is made fresh for every run. We run the PT for the p16 in a water bath since we only run a few slides at a time, but the water bath temp in also monitored. I should say that there is some staining in these slides just not what the control usually looks like. If we repeat the slides they do stain correctly. There is no difference in the slide prep or the staining procedure. Any ideas. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories 20 Northpointe Parkway Suite 100 Amherst, NY 14228 716-250-9235 etx. 232 e-mail cpyse@x-celllab.com -----Original Message----- From: Ross Benik [mailto:ross@premierlab.com] Sent: Wednesday, February 06, 2013 1:26 PM To: Cynthia Pyse Subject: RE: [Histonet] p16 Do you monitor the temperature and humidity inside the Link48? How often do you prepare your retrieval solution/are you using the PT mods? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse Sent: Wednesday, February 06, 2013 11:11 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] p16 Happy Wednesday Everyone Has anyone been having consistency problems with their p16 staining since MTM has been taken over by Ventana? One day the procedure works another day it doesn't. Same procedure, no variation in the temperature on pretreatment, run on the Dako 48, with no errors. Every other IHC works consistently but the p16, anyone have any thoughts? I do make the DAB fresh daily as suggested in the instructions, it is the same kit that has been running. The only thing I can think of is we do sometimes run the p16 twice a day without making fresh DAB, but the instructions say it can be used within a 24 hour period. This problem is driving me crazy! It just doesn't make any sense. Any help would be appreciated. Thanks in advance. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories 20 Northpointe Parkway Suite 100 Amherst, NY 14228 716-250-9235 etx. 232 e-mail cpyse@x-celllab.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rheyna <@t> lumc.edu Wed Feb 6 13:15:35 2013 From: rheyna <@t> lumc.edu (Roger Heyna) Date: Wed Feb 6 13:15:43 2013 Subject: [Histonet] Leica Bond III Opinions Message-ID: <511257770200002300049CAA@gwgwia1.luhs.org> Could I get the opinions of any labs using the Leica Bond III for IHC staining? I'm especially interested to hear from those that switched from Ventana to Leica, but any feedback is appreciated. Few questions that come to mind: Are you happy with the quality of the stains you're performing on the Bond III? Are there antibodies that you were not able to run on the Bond III? Is the machine reliable, or does it break down often, requiring technical service? How arduous is cleaning and managing the cover tiles? Are the three black slide drawers durable? Thank you, Roger Heyna Maywood, IL From brett_connolly <@t> merck.com Wed Feb 6 13:36:21 2013 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Wed Feb 6 13:36:25 2013 Subject: [Histonet] cd11b and cd11c for FFPE mouse tissue Message-ID: Any favorites for CD11b and CD11c for little mice FFPE tissue ? Has anyone used the anti-CD11c antibodies grown in Armenian hamster? Thanks, Brett Brett M. Connolly, Ph.D. Principal Scientist, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From sbaldwin <@t> mhhcc.org Wed Feb 6 13:54:57 2013 From: sbaldwin <@t> mhhcc.org (Sara Baldwin/mhhcc.org) Date: Wed Feb 6 13:54:58 2013 Subject: [Histonet] High complexity test Message-ID: Hi histonetters Is ventana Ultra IHC only doing antibodies no FISH or CISH is this considered High complexity testing? We are doing ER/PR and some others. Thanks Histology/Cytology Supervisor S. Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph 812-996-0210, 0216, Fax 812-996-0232, Pager 812-481-0897, Cell 812-887-3357 From marktarango <@t> gmail.com Wed Feb 6 14:16:40 2013 From: marktarango <@t> gmail.com (Mark Tarango) Date: Wed Feb 6 14:16:45 2013 Subject: [Histonet] High complexity test In-Reply-To: References: Message-ID: If you are just staining the slides and not reading them, then you are NOT performing high complexity testing. The person who reads the slide is doing the high complexity part. Mark On Wed, Feb 6, 2013 at 11:54 AM, Sara Baldwin/mhhcc.org wrote: > Hi histonetters > Is ventana Ultra IHC only doing antibodies no FISH or CISH is this > considered High complexity testing? We are doing ER/PR and some others. > > Thanks > Histology/Cytology Supervisor > S. Kathy Baldwin, SCT (ASCP) > Memorial Hospital and Health Care Center > sbaldwin@mhhcc.org > Ph 812-996-0210, 0216, Fax 812-996-0232, > Pager 812-481-0897, Cell 812-887-3357 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From rjbuesa <@t> yahoo.com Wed Feb 6 14:57:05 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Feb 6 14:57:10 2013 Subject: [Histonet] RE: Histonet Digest, Vol 111, Issue 7 In-Reply-To: <930EB2E8DF68C544873EDD2A3D5F506007D0300CF5@email1.skaggs.net> References: <784e0c98-ce64-4f69-8449-72a2cfa059ae@email1.skaggs.net> <930EB2E8DF68C544873EDD2A3D5F506007D0300CF5@email1.skaggs.net> Message-ID: <1360184225.63751.YahooMailNeo@web163101.mail.bf1.yahoo.com> Please go to www.histosearch.com/rene.html to find proposals and discussions about all commercially available xylene substitutes. Ren? J. From: "Parker, Helayne" To: "'histonet@lists.utsouthwestern.edu'" Sent: Wednesday, February 6, 2013 12:49 PM Subject: [Histonet] RE: Histonet Digest, Vol 111, Issue 7 <<<>>> We use Clear-Rite 3 made by Richard Allan and is purchased at Cardinal.? I do not think it is very expensive we buy in a case of 4 gallons.? It does a decent job for clearing in the processor and de-paraffinizing slides, but we do coverslip out of xylene at the end of the stain line.? Actually we never tried to coverslip out of it Clear-Rite 3 so can not honestly give you a critique in ref to that.? We keep both on hand. Xylene for machine cleaning and coverslipping and Clear-Rite 3 for processing and de-paraffinizing. I like it better than Americlear and ProPar. http://www.cardinalhealth.com/us/en/distributedproducts/ASP/C4405-20.asp?cat=physician Helayne Parker, H.T. (ASCP) Pathology Section Head Cox Medical Center Branson P.O. Box 650, Branson, MO 65615 Phone:? 417-335-7254 Fax:? 417-335-7127 Email:? hparker@skaggs.net Web:? www.coxhealth.com/branson CoxHealth ? ranked one of Missouri's Best Hospitals by U.S. News & World Report COXHEALTH CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law.? Any unauthorized review, use, disclosure or distribution is prohibited.? If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Feb 6 15:01:03 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Feb 6 15:01:07 2013 Subject: [Histonet] Xylene/Paint Thinner In-Reply-To: <930EB2E8DF68C544873EDD2A3D5F506007D0300CF6@email1.skaggs.net> References: <784e0c98-ce64-4f69-8449-72a2cfa059ae@email1.skaggs.net> <930EB2E8DF68C544873EDD2A3D5F506007D0300CF6@email1.skaggs.net> Message-ID: <1360184463.56328.YahooMailNeo@web163104.mail.bf1.yahoo.com> You can find the actual chemical composition if you Google the name and look for the MSDS (as Dr. Richmond has suggested several times). Ren? J. From: "Parker, Helayne" To: "'histonet@lists.utsouthwestern.edu'" Sent: Wednesday, February 6, 2013 1:27 PM Subject: [Histonet] Xylene/Paint Thinner <<<>>> Haha- funny you should say that.? I always swore that Mitaban (for canine demodex mange) was made out of xylene.? Almost looks like watered down xylene.? Well I checked the MSDS and it is but only the inactive ingredient part at a high %.? On a lighter note that orange fragrance d-limonene flea dip is basically the same stuff as the Americlear is. Helayne Parker, H.T. (ASCP) Pathology Section Head Cox Medical Center Branson P.O. Box 650, Branson, MO 65615 Phone:? 417-335-7254 Fax:? 417-335-7127 Email:? hparker@skaggs.net Web:? www.coxhealth.com/branson CoxHealth ? ranked one of Missouri's Best Hospitals by U.S. News & World Report COXHEALTH CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law.? Any unauthorized review, use, disclosure or distribution is prohibited.? If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Feb 6 15:04:14 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Feb 6 15:04:18 2013 Subject: [Histonet] High complexity test In-Reply-To: References: Message-ID: <1360184654.37455.YahooMailNeo@web163105.mail.bf1.yahoo.com> This issue has been discussed at length recently (please go to HistoNet files). The "complexity"?does not deals with the "actual test" but?with the ability of the technician to go above and beyond the "robotic tasks" but also able to think and apply knowledge when something goes wrong. Sometimes dismissal of complexity is rooted on the desire in management to pay less for tasks?that require a higher licensure grade. Ren? J.? From: Sara Baldwin/mhhcc.org To: histonet@lists.utsouthwestern.edu Sent: Wednesday, February 6, 2013 2:54 PM Subject: [Histonet] High complexity test Hi histonetters Is ventana Ultra IHC only doing antibodies no FISH or CISH is this considered High complexity testing?? We are doing ER/PR and some others. Thanks Histology/Cytology Supervisor S. Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph 812-996-0210, 0216, Fax 812-996-0232, Pager 812-481-0897, Cell 812-887-3357 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JEllin <@t> yumaregional.org Wed Feb 6 15:07:24 2013 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Wed Feb 6 15:07:39 2013 Subject: [Histonet] High complexity test In-Reply-To: <1360184654.37455.YahooMailNeo@web163105.mail.bf1.yahoo.com> References: , <1360184654.37455.YahooMailNeo@web163105.mail.bf1.yahoo.com> Message-ID: <8191357C-067A-4E2C-8471-59EE8D59D774@yumaregional.org> I would say this is high complexoty testing and the tech performing this has to have knowledge of the process and troubleshooting in case there is issues with the results. I do not agree with the interpretation some people give,, but this is based on individual institutions Sent from my iPad On Feb 6, 2013, at 2:05 PM, "Rene J Buesa" wrote: > This issue has been discussed at length recently (please go to HistoNet files). > The "complexity" does not deals with the "actual test" but with the ability of the technician to go above and beyond the "robotic tasks" but also able to think and apply knowledge when something goes wrong. > Sometimes dismissal of complexity is rooted on the desire in management to pay less for tasks that require a higher licensure grade. > Ren? J. > > From: Sara Baldwin/mhhcc.org > To: histonet@lists.utsouthwestern.edu > Sent: Wednesday, February 6, 2013 2:54 PM > Subject: [Histonet] High complexity test > > Hi histonetters > Is ventana Ultra IHC only doing antibodies no FISH or CISH is this considered High complexity testing? We are doing ER/PR and some others. > > Thanks > Histology/Cytology Supervisor > S. Kathy Baldwin, SCT (ASCP) > Memorial Hospital and Health Care Center > sbaldwin@mhhcc.org > Ph 812-996-0210, 0216, Fax 812-996-0232, > Pager 812-481-0897, Cell 812-887-3357 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ From marktarango <@t> gmail.com Wed Feb 6 16:07:23 2013 From: marktarango <@t> gmail.com (Mark Tarango) Date: Wed Feb 6 16:07:28 2013 Subject: [Histonet] High complexity test In-Reply-To: <8191357C-067A-4E2C-8471-59EE8D59D774@yumaregional.org> References: <1360184654.37455.YahooMailNeo@web163105.mail.bf1.yahoo.com> <8191357C-067A-4E2C-8471-59EE8D59D774@yumaregional.org> Message-ID: Just to clarify, this is not my interpretation. This is what CAP will tell you when you give them a call. Mark On Wed, Feb 6, 2013 at 1:07 PM, Jesus Ellin wrote: > I would say this is high complexoty testing and the tech performing this > has to have knowledge of the process and troubleshooting in case there is > issues with the results. I do not agree with the interpretation some > people give,, but this is based on individual institutions > > Sent from my iPad > > On Feb 6, 2013, at 2:05 PM, "Rene J Buesa" wrote: > > > This issue has been discussed at length recently (please go to HistoNet > files). > > The "complexity" does not deals with the "actual test" but with the > ability of the technician to go above and beyond the "robotic tasks" but > also able to think and apply knowledge when something goes wrong. > > Sometimes dismissal of complexity is rooted on the desire in management > to pay less for tasks that require a higher licensure grade. > > Ren? J. > > > > From: Sara Baldwin/mhhcc.org > > To: histonet@lists.utsouthwestern.edu > > Sent: Wednesday, February 6, 2013 2:54 PM > > Subject: [Histonet] High complexity test > > > > Hi histonetters > > Is ventana Ultra IHC only doing antibodies no FISH or CISH is this > considered High complexity testing? We are doing ER/PR and some others. > > > > Thanks > > Histology/Cytology Supervisor > > S. Kathy Baldwin, SCT (ASCP) > > Memorial Hospital and Health Care Center > > sbaldwin@mhhcc.org > > Ph 812-996-0210, 0216, Fax 812-996-0232, > > Pager 812-481-0897, Cell 812-887-3357 > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ______________________________________________________________________ > This message is confidential, intended only for the named > recipient(s) and may contain information that is privileged > or exempt from disclosure under applicable law. If you are > not the intended recipient(s), you are notified that the > dissemination, distribution, or copying of this message is > strictly prohibited. If you receive this message in error, > or are not the named recipient(s), please notify the sender > at either the e-mail, fax, address, or telephone number > listed above and delete this e-mail from your computer. > Thank You. > ______________________________________________________________________ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Timothy.Morken <@t> ucsfmedctr.org Wed Feb 6 16:47:22 2013 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Wed Feb 6 16:47:31 2013 Subject: [Histonet] High complexity test In-Reply-To: References: <1360184654.37455.YahooMailNeo@web163105.mail.bf1.yahoo.com> <8191357C-067A-4E2C-8471-59EE8D59D774@yumaregional.org> Message-ID: <761E2B5697F795489C8710BCC72141FF0541FD@ex07.net.ucsf.edu> The CLIA definition of High Complexity testing is not absolute, rather High Complexity Testing is determined by a scored algorithm of the entire "Test System" (preanalytical through Post Analytical). As such, it clearly takes into account the laboratory or other personnel performing all the specimen collection, grossing, processing, cutting in the pre-analytical phase, and the "Testing Personnel" (CLIA Definition) performing the analytical phase (preparing slides, reagents, applying reagents, quality control, etc) and post analytical phase(interpretation) . The Pathologist's role is only part of that and is scored accordingly. CLIA clearly considers the IHC Test System as High Complexity and requires a technologist for the IHC portion with at least and Associates degree (or equivalent, including course and experience in appropriate science and testing) for Testing Personnel. So, I don't think it is correct to dismiss any personnel standards as irrelevant simply because a pathologist will do the interpretation. Note that if ANY High Complexity tests are performed in the lab then the lab must have a CLIA certification for High Complexity Testing. References: CLIA website with the personnel regulations: http://wwwn.cdc.gov/clia/regs/subpart_m.aspx CAP Q&A about personnel standards for IHC, ISH and IF http://www.cap.org/apps/docs/education/lapaudio/pdf/031710_qa.pdf Relevant IHC question reproduced below: Q 17. My question refers more specifically to immunofluorescence, in situ hybridization and immunohistochemistry. Are the techs that perform these tests considered high complexity testing personnel? If the techs are reporting any kind of preliminary result, they must be qualified to do high complexity testing. If all they are doing is applying the stain, then that is considered processing. A: Personnel performing immunofluorescence, immunohistochemistry and in-situ hybridization techniques require qualifications applicable to high complexity testing. Personnel performing histology processing using routine standardized staining procedures (not classified as molecular) do not fall under CLIA as testing personnel and do not have qualification requirements define CLIA website detailing test categorization: http://wwwn.cdc.gov/clia/regs/subpart_a.aspx#493.17 Excerpt here about High vs Moderate complexity (low complexity are basically home use tests) Sec. 493.17 Test categorization. (a) Categorization by criteria. Notices will be published in the Federal Register which list each specific test system, assay, and examination categorized by complexity. Using the seven criteria specified in this paragraph for categorizing tests of moderate or high complexity, each specific laboratory test system, assay, and examination will be graded for level of complexity by assigning scores of 1, 2, or 3 within each criteria. The score of "1" indicates the lowest level of complexity, and the score of "3" indicates the highest level. These scores will be totaled. Test systems, assays or examinations receiving scores of 12 or less will be categorized as moderate complexity, while those receiving scores above 12 will be categorized as high complexity. Note: A score of "2" will be assigned to a criteria heading when the characteristics for a particular test are intermediate between the descriptions listed for scores of "1" and "3." (1) Knowledge. (i) Score 1. (A) Minimal scientific and technical knowledge is required to perform the test; and (B) Knowledge required to perform the test may be obtained through on-the-job instruction. (ii) Score 3. Specialized scientific and technical knowledge is essential to perform preanalytic, analytic or postanalytic phases of the testing. (2) Training and experience. (i) Score 1. (A) Minimal training is required for preanalytic, analytic and postanalytic phases of the testing process; and (B) Limited experience is required to perform the test. (ii) Score 3. (A) Specialized training is essential to perform the preanalytic, analytic or postanalytic testing process; or (B) Substantial experience may be necessary for analytic test performance. (3) Reagents and materials preparation. (i) Score 1. (A) Reagents and materials are generally stable and reliable; and (B) Reagents and materials are prepackaged, or premeasured, or Require no special handling, precautions or storage conditions. (ii) Score 3. (A) Reagents and materials may be labile and may require special handling to assure reliability; or (B) Reagents and materials preparation may include manual steps such as gravimetric or volumetric measurements. (4) Characteristics of operational steps. (i) Score 1. Operational steps are either automatically executed (such as pipetting, temperature monitoring, or timing of steps), or are easily controlled. (ii) Score 3. Operational steps in the testing process require close monitoring or control, and may require special specimen preparation, precise temperature control or timing of procedural steps, accurate pipetting, or extensive calculations. (5) Calibration, quality control, and proficiency testing materials. (i) Score 1. (A) Calibration materials are stable and readily available; (B) Quality control materials are stable and readily available; and (C) External proficiency testing materials, when available, are stable. (ii) Score 3. (A) Calibration materials, if available, may be labile; (B) Quality control materials may be labile, or not available; or (C) External proficiency testing materials, if available, may be labile. (6) Test system troubleshooting and equipment maintenance. (i) Score 1. (A) Test system troubleshooting is automatic or self- correcting, or clearly described or requires minimal judgment; and (B) Equipment maintenance is provided by the manufacturer, is seldom needed, or can easily be performed. (ii) Score 3. (A) Troubleshooting is not automatic and requires decision-making and direct intervention to resolve most problems; or (B) Maintenance requires special knowledge, skills, and abilities. (7) Interpretation and judgment. (i) Score 1. (A) Minimal interpretation and judgment are required to perform preanalytic, analytic and postanalytic processes; and (B) Resolution of problems requires limited independent interpretation and judgment; and (ii) Score 3. (A) Extensive independent interpretation and judgment are required to perform the preanalytic, analytic or postanalytic processes; and (B) Resolution of problems requires extensive interpretation and judgment. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Tarango Sent: Wednesday, February 06, 2013 2:07 PM To: Jesus Ellin Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] High complexity test Just to clarify, this is not my interpretation. This is what CAP will tell you when you give them a call. Mark On Wed, Feb 6, 2013 at 1:07 PM, Jesus Ellin wrote: > I would say this is high complexoty testing and the tech performing > this has to have knowledge of the process and troubleshooting in case > there is issues with the results. I do not agree with the > interpretation some people give,, but this is based on individual > institutions > > Sent from my iPad > > On Feb 6, 2013, at 2:05 PM, "Rene J Buesa" wrote: > > > This issue has been discussed at length recently (please go to > > HistoNet > files). > > The "complexity" does not deals with the "actual test" but with the > ability of the technician to go above and beyond the "robotic tasks" > but also able to think and apply knowledge when something goes wrong. > > Sometimes dismissal of complexity is rooted on the desire in > > management > to pay less for tasks that require a higher licensure grade. > > Ren? J. > > > > From: Sara Baldwin/mhhcc.org > > To: histonet@lists.utsouthwestern.edu > > Sent: Wednesday, February 6, 2013 2:54 PM > > Subject: [Histonet] High complexity test > > > > Hi histonetters > > Is ventana Ultra IHC only doing antibodies no FISH or CISH is this > considered High complexity testing? We are doing ER/PR and some others. > > > > Thanks > > Histology/Cytology Supervisor > > S. Kathy Baldwin, SCT (ASCP) > > Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph > > 812-996-0210, 0216, Fax 812-996-0232, Pager 812-481-0897, Cell > > 812-887-3357 _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ______________________________________________________________________ > This message is confidential, intended only for the named > recipient(s) and may contain information that is privileged or exempt > from disclosure under applicable law. If you are not the intended > recipient(s), you are notified that the dissemination, distribution, > or copying of this message is strictly prohibited. If you receive > this message in error, or are not the named recipient(s), please > notify the sender at either the e-mail, fax, address, or telephone > number listed above and delete this e-mail from your computer. > Thank You. > ______________________________________________________________________ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ccrowder <@t> vetmed.lsu.edu Wed Feb 6 17:12:53 2013 From: ccrowder <@t> vetmed.lsu.edu (Cheryl Crowder) Date: Wed Feb 6 17:12:59 2013 Subject: [Histonet] Used tissue processors Message-ID: Hi - Would the person who contacted me about a used processor please e-mail again. Thank you. Cheryl Cheryl Crowder, BA, HTL(ASCP) Crowder Histology Consulting 4952 Alvin Dark Ave. Baton Rouge, LA 70820 (225) 772-2865 From mariamichails <@t> gmail.com Wed Feb 6 17:26:35 2013 From: mariamichails <@t> gmail.com (Maria Michails) Date: Wed Feb 6 17:26:38 2013 Subject: [Histonet] need prepared slides of carcinogenicity--North American supplier?? Message-ID: Hello I am in need of prepared slides, stained for confocal microscopy or unstained (DIC) of the following samples: Primary - non-lymphocytic leukemia - renal (kidney) cancer Secondary - breast, bone, or brain cancer - lymphocytic leukemia - Lymphoma (non-Hodgkin's) - gall bladder or liver cancer - lung cancer I am having trouble locating a supplier. If anyone can direct me to a North American supplier where I can purchase slides I would really appreciate it. I've done an extensive search on google but none of the suppliers (mostly educational) have what I am in need of. Thank you. Maria Michails Central Michigan University From Joyce.Weems <@t> emoryhealthcare.org Wed Feb 6 20:32:47 2013 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Wed Feb 6 20:33:01 2013 Subject: [Histonet] High complexity test In-Reply-To: <761E2B5697F795489C8710BCC72141FF0541FD@ex07.net.ucsf.edu> References: <1360184654.37455.YahooMailNeo@web163105.mail.bf1.yahoo.com> <8191357C-067A-4E2C-8471-59EE8D59D774@yumaregional.org> <761E2B5697F795489C8710BCC72141FF0541FD@ex07.net.ucsf.edu> Message-ID: As always, thanks Tim!! Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Wednesday, February 06, 2013 5:47 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] High complexity test The CLIA definition of High Complexity testing is not absolute, rather High Complexity Testing is determined by a scored algorithm of the entire "Test System" (preanalytical through Post Analytical). As such, it clearly takes into account the laboratory or other personnel performing all the specimen collection, grossing, processing, cutting in the pre-analytical phase, and the "Testing Personnel" (CLIA Definition) performing the analytical phase (preparing slides, reagents, applying reagents, quality control, etc) and post analytical phase(interpretation) . The Pathologist's role is only part of that and is scored accordingly. CLIA clearly considers the IHC Test System as High Complexity and requires a technologist for the IHC portion with at least and Associates degree (or equivalent, including course and experience in appropriate science and testing) for Testing Personnel. So, I don't think it is correct to dismiss any personnel standards as irrelevant simply because a pathologist will do the interpretation. Note that if ANY High Complexity tests are performed in the lab then the lab must have a CLIA certification for High Complexity Testing. References: CLIA website with the personnel regulations: http://wwwn.cdc.gov/clia/regs/subpart_m.aspx CAP Q&A about personnel standards for IHC, ISH and IF http://www.cap.org/apps/docs/education/lapaudio/pdf/031710_qa.pdf Relevant IHC question reproduced below: Q 17. My question refers more specifically to immunofluorescence, in situ hybridization and immunohistochemistry. Are the techs that perform these tests considered high complexity testing personnel? If the techs are reporting any kind of preliminary result, they must be qualified to do high complexity testing. If all they are doing is applying the stain, then that is considered processing. A: Personnel performing immunofluorescence, immunohistochemistry and in-situ hybridization techniques require qualifications applicable to high complexity testing. Personnel performing histology processing using routine standardized staining procedures (not classified as molecular) do not fall under CLIA as testing personnel and do not have qualification requirements define CLIA website detailing test categorization: http://wwwn.cdc.gov/clia/regs/subpart_a.aspx#493.17 Excerpt here about High vs Moderate complexity (low complexity are basically home use tests) Sec. 493.17 Test categorization. (a) Categorization by criteria. Notices will be published in the Federal Register which list each specific test system, assay, and examination categorized by complexity. Using the seven criteria specified in this paragraph for categorizing tests of moderate or high complexity, each specific laboratory test system, assay, and examination will be graded for level of complexity by assigning scores of 1, 2, or 3 within each criteria. The score of "1" indicates the lowest level of complexity, and the score of "3" indicates the highest level. These scores will be totaled. Test systems, assays or examinations receiving scores of 12 or less will be categorized as moderate complexity, while those receiving scores above 12 will be categorized as high complexity. Note: A score of "2" will be assigned to a criteria heading when the characteristics for a particular test are intermediate between the descriptions listed for scores of "1" and "3." (1) Knowledge. (i) Score 1. (A) Minimal scientific and technical knowledge is required to perform the test; and (B) Knowledge required to perform the test may be obtained through on-the-job instruction. (ii) Score 3. Specialized scientific and technical knowledge is essential to perform preanalytic, analytic or postanalytic phases of the testing. (2) Training and experience. (i) Score 1. (A) Minimal training is required for preanalytic, analytic and postanalytic phases of the testing process; and (B) Limited experience is required to perform the test. (ii) Score 3. (A) Specialized training is essential to perform the preanalytic, analytic or postanalytic testing process; or (B) Substantial experience may be necessary for analytic test performance. (3) Reagents and materials preparation. (i) Score 1. (A) Reagents and materials are generally stable and reliable; and (B) Reagents and materials are prepackaged, or premeasured, or Require no special handling, precautions or storage conditions. (ii) Score 3. (A) Reagents and materials may be labile and may require special handling to assure reliability; or (B) Reagents and materials preparation may include manual steps such as gravimetric or volumetric measurements. (4) Characteristics of operational steps. (i) Score 1. Operational steps are either automatically executed (such as pipetting, temperature monitoring, or timing of steps), or are easily controlled. (ii) Score 3. Operational steps in the testing process require close monitoring or control, and may require special specimen preparation, precise temperature control or timing of procedural steps, accurate pipetting, or extensive calculations. (5) Calibration, quality control, and proficiency testing materials. (i) Score 1. (A) Calibration materials are stable and readily available; (B) Quality control materials are stable and readily available; and (C) External proficiency testing materials, when available, are stable. (ii) Score 3. (A) Calibration materials, if available, may be labile; (B) Quality control materials may be labile, or not available; or (C) External proficiency testing materials, if available, may be labile. (6) Test system troubleshooting and equipment maintenance. (i) Score 1. (A) Test system troubleshooting is automatic or self- correcting, or clearly described or requires minimal judgment; and (B) Equipment maintenance is provided by the manufacturer, is seldom needed, or can easily be performed. (ii) Score 3. (A) Troubleshooting is not automatic and requires decision-making and direct intervention to resolve most problems; or (B) Maintenance requires special knowledge, skills, and abilities. (7) Interpretation and judgment. (i) Score 1. (A) Minimal interpretation and judgment are required to perform preanalytic, analytic and postanalytic processes; and (B) Resolution of problems requires limited independent interpretation and judgment; and (ii) Score 3. (A) Extensive independent interpretation and judgment are required to perform the preanalytic, analytic or postanalytic processes; and (B) Resolution of problems requires extensive interpretation and judgment. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Tarango Sent: Wednesday, February 06, 2013 2:07 PM To: Jesus Ellin Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] High complexity test Just to clarify, this is not my interpretation. This is what CAP will tell you when you give them a call. Mark On Wed, Feb 6, 2013 at 1:07 PM, Jesus Ellin wrote: > I would say this is high complexoty testing and the tech performing > this has to have knowledge of the process and troubleshooting in case > there is issues with the results. I do not agree with the > interpretation some people give,, but this is based on individual > institutions > > Sent from my iPad > > On Feb 6, 2013, at 2:05 PM, "Rene J Buesa" wrote: > > > This issue has been discussed at length recently (please go to > > HistoNet > files). > > The "complexity" does not deals with the "actual test" but with the > ability of the technician to go above and beyond the "robotic tasks" > but also able to think and apply knowledge when something goes wrong. > > Sometimes dismissal of complexity is rooted on the desire in > > management > to pay less for tasks that require a higher licensure grade. > > Ren? J. > > > > From: Sara Baldwin/mhhcc.org > > To: histonet@lists.utsouthwestern.edu > > Sent: Wednesday, February 6, 2013 2:54 PM > > Subject: [Histonet] High complexity test > > > > Hi histonetters > > Is ventana Ultra IHC only doing antibodies no FISH or CISH is this > considered High complexity testing? We are doing ER/PR and some others. > > > > Thanks > > Histology/Cytology Supervisor > > S. Kathy Baldwin, SCT (ASCP) > > Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph > > 812-996-0210, 0216, Fax 812-996-0232, Pager 812-481-0897, Cell > > 812-887-3357 _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ______________________________________________________________________ > This message is confidential, intended only for the named > recipient(s) and may contain information that is privileged or exempt > from disclosure under applicable law. If you are not the intended > recipient(s), you are notified that the dissemination, distribution, > or copying of this message is strictly prohibited. If you receive > this message in error, or are not the named recipient(s), please > notify the sender at either the e-mail, fax, address, or telephone > number listed above and delete this e-mail from your computer. > Thank You. > ______________________________________________________________________ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From rcartun <@t> harthosp.org Wed Feb 6 21:36:27 2013 From: rcartun <@t> harthosp.org (Richard Cartun) Date: Wed Feb 6 21:36:38 2013 Subject: [Histonet] P16 on Leica Bond Message-ID: <5112DAEB020000770003694C@gwmail1.harthosp.org> When we obtained the p16 antibody from mtm Laboratories we used it a dilution of 1:10 on the Bond Max. I hope that the concentration will not change now that Ventana is selling it. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Assistant Director, Anatomic Pathology Director, Biospecimen Collection Programs Hartford Hospital 80 Seymour Street Hartford, CT 06012 (860) 545-1596 (860) 545-2204 Fax >>> Kathryn Maddox 02/06/13 12:13 PM >>> If anyone out there is performing p16 on the Leica Bond immunostainer, could you please tell me where you purchase your antibody and what protocol you are using? Thanks so much! Kathy Maddox HT{ASCP} Lake Charles, Louisiana _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ewj <@t> pigsqq.org Wed Feb 6 22:27:05 2013 From: ewj <@t> pigsqq.org (ewj) Date: Wed Feb 6 22:27:21 2013 Subject: [Histonet] Xylene Substitutes In-Reply-To: <1360163799.83270.YahooMailNeo@web163106.mail.bf1.yahoo.com> References: <1360101506.39015.YahooMailNeo@web125606.mail.ne1.yahoo.com> <1360163799.83270.YahooMailNeo@web163106.mail.bf1.yahoo.com> Message-ID: <51132D19.6010704@pigsqq.org> We have a small lab at a university in Beijing where we do diagnostic histopath for swine diseases. The lab is shared with graduate students who make histomorphologic measurements on tissues like gut and muscle as part of their research. We have a Sakura autostainer. Last summer one student left all of the caps off of all of the containers in the DRS autostainer over a hot summer weekend and vaporized a very large amount of xylene and ethanol into the room and out into the hallway which is on a floor shared with clerical staff one of whom was in early stage pregnancy. There was quite a furor ensuing, and no matter what the cause, the foreigner in the building and his heavy use of the laboratory was to blame. I have the support of the leadership/management at the building and weathered the incident but it was not a comfortable situation. Reducing the use of xylene is very attractive to us. We have now been using Rene's hot water detergent method for some time. I have noticed that when I do the process we get more reliable removal of the paraffin than if my staff does it. Also one of my workers has small sensitive hands. My hands are toughened up from growing up on a farm and working in the oil fields, and working with cattle and horses for quite a few years, so the hot water doesnt bother me much, but it is a problem for her and she is an excellent and valuable staff member and really I should be concerned about scalding any one. Rene' had suggested that I could get one of those restaurant coffee 'vats' with a spigot on the bottom to make and hold the hot water detergent solution, but those are not so easy to come by. I have found some old sterilizers which are essentially glorified pressure cookers with some automatic control that were cast aside. These I have rigged up with pt100 thermocouples and PID thermocontrollers and use them to heat the water. They have a drain port so I have also affixed them with heat tolerant solenoid valves for input and to drain the slide container, and water level sensors, all of which are connected to a computer driven data access module via relays so that process can be automated and repeatable and have tweakable cycles. It's easier for me to procure such stuff in Beijing than it is to find t-shirts that fit me. This also provides ready access to crisp hot water which is great for cleaning up paraffin from tissue block molds and tables and floors, clearing plugged cleaning lines from the VIP5 (like when students fail to drain the retort prior to retrieving their cassettes), and what not. We dont have hot running tap water in the university lab. (It's a luxury in homes as well but much more common in recent years.) I havent got around to converting the VIP5 to 2-propanol and mineral oil. It's on a different floor with good ventilation and I have enough trouble getting the students to read the sign to drain the retort (in chinese), so I am not needing another level of complexity there just yet. I have been fiddling with the detergent solution and have found what does not work and also found a reasonable result with an imported powdered dishwasher soap. We used a water bath at first which is a real pain to use, is slow, and unpredictable. Predictable hot water is important. We find much fewer problems with lost sections particularly brain with this method than with the xylene-alcohol series. We do pig work primarily so we run lots of brain sections looking for PRV and CSF and strep. I am glad that Rene' continues to push his xylene-free concept. It has helped me immensely Wayne Johnson Enable Ag Tech Consulting Beijing On 3:59, Rene J Buesa wrote: > The best solution to eliminate xylene is to use isopropyl alcohol mixed with mineral oil. > Xylene can be eliminated from staining by dewaxing with 2% aq. sol. of dishwasher soap. > Before coverslipping oven dry the stained sections and cover directly. > To clean tissue processors use a 2% mixture of strong lab lab-ware detergent. > Do all of the above and your lab will be xylene free. > Ren? J. > > From: Tom McNemar > To: 'Adrienne Anderson'; "histonet@lists.utsouthwestern.edu" > Sent: Wednesday, February 6, 2013 5:36 AM > Subject: RE: [Histonet] Xylene Substitutes > > Not familiar with SubX but we have used Americlear for many years with good results. You just can't beat Xylene for some things though and still keep a little around. > > Tom McNemar, HT(ASCP) > Histology Co-ordinator > Licking Memorial Health Systems > (740) 348-4163 > (740) 348-4166 > tmcnemar@lmhealth.org > http://www.lmhealth.org/ > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Adrienne Anderson > Sent: Tuesday, February 05, 2013 4:58 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Xylene Substitutes > > Hello all, > > My lab is looking into xylene substitutes, and I'd love some feedback on what other labs are using. We currently use SubX, but are there other items out there more economical? > > Thanks, > Adrienne > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From MICHELLE.LAMPHERE <@t> childrens.com Thu Feb 7 08:54:00 2013 From: MICHELLE.LAMPHERE <@t> childrens.com (Michelle Lamphere) Date: Thu Feb 7 08:54:03 2013 Subject: [Histonet] RE: Leica Bond III Opinions In-Reply-To: References: Message-ID: I would love to hear any feedback that you get from this query. Michelle M Lamphere, HT (ASCP) Senior Tech, Histology Children's Medical Center 1935 Medical District Drive Dallas, TX 75235 Office :214-456-2798 Histology: 214-456-2318 Fax: 214-456-0779 Could I get the opinions of any labs using the Leica Bond III for IHC staining? I'm especially interested to hear from those that switched from Ventana to Leica, but any feedback is appreciated. Few questions that come to mind: Are you happy with the quality of the stains you're performing on the Bond III? Are there antibodies that you were not able to run on the Bond III? Is the machine reliable, or does it break down often, requiring technical service? How arduous is cleaning and managing the cover tiles? Are the three black slide drawers durable? Thank you, Roger Heyna Maywood, IL Please consider the environment before printing this e-mail. This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. From thigginsht <@t> msn.com Thu Feb 7 08:58:12 2013 From: thigginsht <@t> msn.com (Tim Higgins) Date: Thu Feb 7 08:58:16 2013 Subject: [Histonet] RE: High complexity test In-Reply-To: References: Message-ID: Hey Kathy, It is not considered a High Complexity test on the staining side of it. You don't need any special degree or certification, I know we all want our profession to fetch more money and respect and a good way is make a mountain out of molehill to use an old saying. Now here is the gray area, the high complexity could come with the resulting of the stains, such as ER, PR, HER2 using an semi-quantitative detection and algorithms. The simple "robotics" of staining is not considered high complexity, you do need knowledge and understanding of how detection kits work and antibodies react so and so forth, but its still not considered high complexity. Best way is to call CAP or whatever governing body your under and ask the question and document who you spoke with if there is nothing in writing. Tim H. > > > From: Sara Baldwin/mhhcc.org > > > To: histonet@lists.utsouthwestern.edu > > > Sent: Wednesday, February 6, 2013 2:54 PM > > > Subject: [Histonet] High complexity test > > > > > > Hi histonetters > > > Is ventana Ultra IHC only doing antibodies no FISH or CISH is this > > considered High complexity testing? We are doing ER/PR and some others. > > > > > > Thanks > > > Histology/Cytology Supervisor > > > S. Kathy Baldwin, SCT (ASCP) > > > Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph > > > 812-996-0210, 0216, Fax 812-996-0232, Pager 812-481-0897, Cell > > > 812-887-3357 _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ______________________________________________________________________ > > This message is confidential, intended only for the named > > recipient(s) and may contain information that is privileged or exempt > > from disclosure under applicable law. If you are not the intended > > recipient(s), you are notified that the dissemination, distribution, > > or copying of this message is strictly prohibited. If you receive > > this message in error, or are not the named recipient(s), please > > notify the sender at either the e-mail, fax, address, or telephone > > number listed above and delete this e-mail from your computer. > > Thank You. > > ______________________________________________________________________ > > From TMcNemar <@t> lmhealth.org Thu Feb 7 09:14:46 2013 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Thu Feb 7 09:14:53 2013 Subject: [Histonet] Processor dehydration cycles.. Message-ID: Hello all, I was wondering what most people use as the first reagent after the formalins on their tissue processor? We have always used a sequence of 70%, 80%, 95%, and 100% but is anyone using 80% or even 95% to start their dehydration? Thanks in advance. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org ________________________________ This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From bakerj <@t> med.umich.edu Thu Feb 7 09:19:39 2013 From: bakerj <@t> med.umich.edu (Baker, John) Date: Thu Feb 7 09:20:51 2013 Subject: [Histonet] embedding problem Message-ID: <5F1442240F9B494A95CF983066289F77240044D6@UHEXMBSPR13.umhs.med.umich.edu> I had to sign up again for the Histonet so I am not sure if this question went out so will resend. thanks Hello Histonetters, We are trying to embed a polycarbonate device with soft tissue attached to look at the implant interface. The problem is that with several standard protocols for pmma processing the clearing agent (methyl salicylate or xylene) and the methacrylate monomer dissolves the polycarbonate. Does anyone have any experience trying process such a thing, an embedding media (pmma, OCT for cryo, etc.), and then a method of sectioning it keeping the interface intact? Thanks in advance for any suggestions! John John A. Baker The University of Michigan Orthopaedic Research Laboratories Histology Unit 109 Zina Pitcher Place, 2218 BSRB Ann Arbor, MI 48109-2200 734-936-1635 ********************************************************** Electronic Mail is not secure, may not be read every day, and should not be used for urgent or sensitive issues From rjbuesa <@t> yahoo.com Thu Feb 7 09:22:34 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Feb 7 09:22:40 2013 Subject: [Histonet] High complexity test In-Reply-To: <761E2B5697F795489C8710BCC72141FF0541FD@ex07.net.ucsf.edu> References: <1360184654.37455.YahooMailNeo@web163105.mail.bf1.yahoo.com> <8191357C-067A-4E2C-8471-59EE8D59D774@yumaregional.org> <761E2B5697F795489C8710BCC72141FF0541FD@ex07.net.ucsf.edu> Message-ID: <1360250554.2421.YahooMailNeo@web163101.mail.bf1.yahoo.com> I only hope that Tim's posting sets this issue to rest. The histotech doing IHC, FISH, or grossing and some other complex tasks?has to have special training and studies because all those are high complexity tests. Even those histotechs reading FISH results (counting the reactive nuclei for latter signing by the pathologist) have to receive special training. I will just point out again that some administrators try to "underrate" these tests in order to train "their monkeys" and?pay less even when the ratio billing/cost is extremely high. Management greed drives sometimes these issues. Ren? J. From: "Morken, Timothy" To: "histonet@lists.utsouthwestern.edu" Sent: Wednesday, February 6, 2013 5:47 PM Subject: RE: [Histonet] High complexity test The CLIA definition of High Complexity testing is not absolute, rather High Complexity Testing is determined by a scored algorithm of the entire "Test System" (preanalytical through Post Analytical). As such, it clearly takes into account the laboratory or other personnel performing all the specimen collection, grossing, processing, cutting in the pre-analytical phase, and the "Testing Personnel" (CLIA Definition) performing the analytical phase (preparing slides, reagents, applying reagents, quality control, etc) and post analytical phase(interpretation) . The Pathologist's role is only part of that and is scored accordingly. CLIA clearly considers the IHC Test System as High Complexity and requires a technologist for the IHC portion with at least and Associates degree (or equivalent, including course and experience in appropriate science and testing) for Testing Personnel. So, I don't think it is correct to dismiss any personnel standards as irrelevant simply because a pathologist will do the interpretation. Note that if ANY High Complexity tests are performed in the lab then the lab must have a CLIA certification for High Complexity Testing. References: CLIA website with the personnel regulations:? http://wwwn.cdc.gov/clia/regs/subpart_m.aspx CAP Q&A about personnel standards for IHC, ISH and IF? http://www.cap.org/apps/docs/education/lapaudio/pdf/031710_qa.pdf Relevant IHC question reproduced below: Q 17. My question refers more specifically to immunofluorescence, in situ hybridization and immunohistochemistry. Are the techs that perform these tests considered high complexity testing personnel?? If the techs are reporting any kind of preliminary result, they must be qualified to do high complexity testing.? If all they are doing is applying the stain, then that is considered processing. A: Personnel performing immunofluorescence, immunohistochemistry and in-situ hybridization techniques require qualifications applicable to high complexity testing. Personnel performing histology processing using routine standardized staining procedures (not classified as molecular) do not fall under CLIA as testing personnel and do not have qualification requirements define CLIA website detailing test categorization: http://wwwn.cdc.gov/clia/regs/subpart_a.aspx#493.17 Excerpt here about High vs Moderate complexity (low complexity are basically home use tests) ? ? Sec. 493.17? Test categorization. ? ? ? ? ? (a) Categorization by criteria. Notices will be published in the ? ? ? Federal Register which list each specific test system, assay, and ? ? ? examination categorized by complexity. Using the seven criteria ? ? ? specified in this paragraph for categorizing tests of moderate or high ? ? ? complexity, each specific laboratory test system, assay, and examination ? ? ? will be graded for level of complexity by assigning scores of 1, 2, or 3 ? ? ? within each criteria. The score of "1" indicates the lowest level of ? ? ? complexity, and the score of "3" indicates the highest level. These ? ? ? scores will be totaled. Test systems, assays or examinations receiving ? ? ? scores of 12 or less will be categorized as moderate complexity, while ? ? ? those receiving scores above 12 will be categorized as high complexity. ? ? ? ? ? Note: A score of "2" will be assigned to a criteria heading when ? ? ? the characteristics for a particular test are intermediate between the ? ? ? descriptions listed for scores of "1" and "3." ? ? ? ? ? (1) Knowledge. ? ? (i) Score 1. (A) Minimal scientific and technical knowledge is ? ? ? required to perform the test; and ? ? (B) Knowledge required to perform the test may be obtained through ? ? ? on-the-job instruction. ? ? (ii) Score 3. Specialized scientific and technical knowledge is ? ? ? essential to perform preanalytic, analytic or postanalytic phases of the ? ? ? testing. ? ? (2) Training and experience. ? ? (i) Score 1. (A) Minimal training is required for preanalytic, ? ? ? analytic and postanalytic phases of the testing process; and ? ? (B) Limited experience is required to perform the test. ? ? (ii) Score 3. (A) Specialized training is essential to perform the ? ? ? preanalytic, analytic or postanalytic testing process; or ? ? (B) Substantial experience may be necessary for analytic test ? ? ? performance. ? ? (3) Reagents and materials preparation. ? ? (i) Score 1. (A) Reagents and materials are generally stable and ? ? ? reliable; and ? ? (B) Reagents and materials are prepackaged, or premeasured, or ? ? ? Require no special handling, precautions or storage conditions. ? ? (ii) Score 3. (A) Reagents and materials may be labile and may ? ? ? require special handling to assure reliability; or ? ? (B) Reagents and materials preparation may include manual steps such ? ? ? as gravimetric or volumetric measurements. ? ? (4) Characteristics of operational steps. (i) Score 1. Operational ? ? ? steps are either automatically executed (such as pipetting, temperature ? ? ? monitoring, or timing of steps), or are easily controlled. ? ? (ii) Score 3. Operational steps in the testing process require close ? ? ? monitoring or control, and may require special specimen preparation, ? ? ? precise temperature control or timing of procedural steps, accurate ? ? ? pipetting, or extensive calculations. ? ? (5) Calibration, quality control, and proficiency testing materials. ? ? (i) Score 1. (A) Calibration materials are stable and readily ? ? ? available; ? ? (B) Quality control materials are stable and readily available; and ? ? (C) External proficiency testing materials, when available, are ? ? ? stable. ? ? (ii) Score 3. (A) Calibration materials, if available, may be ? ? ? labile; ? ? (B) Quality control materials may be labile, or not available; or ? ? (C) External proficiency testing materials, if available, may be ? ? ? labile. ? ? (6) Test system troubleshooting and equipment maintenance. ? ? (i) Score 1. (A) Test system troubleshooting is automatic or self- ? ? ? correcting, or clearly described or requires minimal judgment; and ? ? (B) Equipment maintenance is provided by the manufacturer, is seldom ? ? ? needed, or can easily be performed. ? ? (ii) Score 3. (A) Troubleshooting is not automatic and requires ? ? ? decision-making and direct intervention to resolve most problems; or ? ? (B) Maintenance requires special knowledge, skills, and abilities. ? ? (7) Interpretation and judgment. (i) Score 1. (A) Minimal ? ? ? interpretation and judgment are required to perform preanalytic, ? ? ? analytic and postanalytic processes; and ? ? (B) Resolution of problems requires limited independent ? ? ? interpretation and judgment; and ? ? (ii) Score 3. (A) Extensive independent interpretation and judgment ? ? ? are required to perform the preanalytic, analytic or postanalytic ? ? ? processes; and ? ? (B) Resolution of problems requires extensive interpretation and ? ? ? judgment. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Tarango Sent: Wednesday, February 06, 2013 2:07 PM To: Jesus Ellin Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] High complexity test Just to clarify, this is not my interpretation.? This is what CAP will tell you when you give them a call. Mark On Wed, Feb 6, 2013 at 1:07 PM, Jesus Ellin wrote: > I would say this is high complexoty testing and the tech performing > this has to have knowledge of the process and troubleshooting in case > there is issues with the results.? I do not agree with the > interpretation some people give,, but this is based on individual > institutions > > Sent from my iPad > > On Feb 6, 2013, at 2:05 PM, "Rene J Buesa" wrote: > > > This issue has been discussed at length recently (please go to > > HistoNet > files). > > The "complexity" does not deals with the "actual test" but with the > ability of the technician to go above and beyond the "robotic tasks" > but also able to think and apply knowledge when something goes wrong. > > Sometimes dismissal of complexity is rooted on the desire in > > management > to pay less for tasks that require a higher licensure grade. > > Ren? J. > > > > From: Sara Baldwin/mhhcc.org > > To: histonet@lists.utsouthwestern.edu > > Sent: Wednesday, February 6, 2013 2:54 PM > > Subject: [Histonet] High complexity test > > > > Hi histonetters > > Is ventana Ultra IHC only doing antibodies no FISH or CISH is this > considered High complexity testing?? We are doing ER/PR and some others. > > > > Thanks > > Histology/Cytology Supervisor > > S. Kathy Baldwin, SCT (ASCP) > > Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph > > 812-996-0210, 0216, Fax 812-996-0232, Pager 812-481-0897, Cell > > 812-887-3357 _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ______________________________________________________________________ > This message is confidential, intended only for the named > recipient(s) and may contain information that is privileged or exempt > from disclosure under applicable law.? If you are not the intended > recipient(s), you are notified that the dissemination, distribution, > or copying of this message is strictly prohibited.? If you receive > this message in error, or are not the named recipient(s), please > notify the sender at either the e-mail, fax, address, or telephone > number listed above and delete this e-mail from your computer. > Thank You. > ______________________________________________________________________ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Lisa.White3 <@t> va.gov Thu Feb 7 09:23:37 2013 From: Lisa.White3 <@t> va.gov (White, Lisa M.) Date: Thu Feb 7 09:25:34 2013 Subject: [Histonet] Xylene/Paint Thinner Message-ID: <2B2ECF33934F5D4996D8BE03EFDF39760AE21D91@VHAV09MSGA3.v09.med.va.gov> If you look on the paint thinner isle at your local Lowe's. You will find Xylene on the shelf. The first time I saw it was a shock. Wonder if the home improvement weekend warriors know what they are getting all over their hands????? Lisa White, HT(ASCP) Supervisory HT James H. Quillen VAMC PO Box 4000 Corner of Veterans Way and Lamont PLMS 113 Mountain Home, TN 37684 423-979-3567 423-979-3401 fax From Joyce.Weems <@t> emoryhealthcare.org Thu Feb 7 09:31:52 2013 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Thu Feb 7 09:32:01 2013 Subject: [Histonet] RE: Processor dehydration cycles.. In-Reply-To: References: Message-ID: You have to be careful or the salts will precipitate out of the formalin if you start too high. I wouldn't go any higher than 70. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Thursday, February 07, 2013 10:15 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Processor dehydration cycles.. Hello all, I was wondering what most people use as the first reagent after the formalins on their tissue processor? We have always used a sequence of 70%, 80%, 95%, and 100% but is anyone using 80% or even 95% to start their dehydration? Thanks in advance. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org ________________________________ This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From rjbuesa <@t> yahoo.com Thu Feb 7 09:32:46 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Feb 7 09:32:50 2013 Subject: [Histonet] Processor dehydration cycles.. In-Reply-To: References: Message-ID: <1360251166.27691.YahooMailNeo@web163104.mail.bf1.yahoo.com> I usually started with 80%EthOL but it does not "harm" starting with 70%EthOL and it is even better, from the?theoretically view point. So if you have the "space" in your protocol, keep the 70%EthOL Ren? J. From: Tom McNemar To: "histonet@lists.utsouthwestern.edu" Sent: Thursday, February 7, 2013 10:14 AM Subject: [Histonet] Processor dehydration cycles.. Hello all, I was wondering what most people use as the first reagent after the formalins on their tissue processor?? We have always used a sequence of 70%, 80%, 95%, and 100% but is anyone using 80% or even 95% to start their dehydration? Thanks in advance. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org ________________________________ This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Thu Feb 7 09:36:30 2013 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Thu Feb 7 09:36:38 2013 Subject: [Histonet] RE: embedding problem In-Reply-To: <5F1442240F9B494A95CF983066289F77240044D6@UHEXMBSPR13.umhs.med.umich.edu> References: <5F1442240F9B494A95CF983066289F77240044D6@UHEXMBSPR13.umhs.med.umich.edu> Message-ID: <14E2C6176416974295479C64A11CB9AE016411AB4641@SBS2K8.premierlab.local> John Have you tried paraffin? I know that we have been able to section some plastic devices with paraffin sections, it may be worth a try. Looks like the device you are working with is compatable with xylene, we have found in some cases that we need to use a xylene subsititute. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 881-0763 cell (303) 682-9060 fax liz@premierlab.com Ship to address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Baker, John [bakerj@med.umich.edu] Sent: Thursday, February 07, 2013 8:19 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] embedding problem I had to sign up again for the Histonet so I am not sure if this question went out so will resend. thanks Hello Histonetters, We are trying to embed a polycarbonate device with soft tissue attached to look at the implant interface. The problem is that with several standard protocols for pmma processing the clearing agent (methyl salicylate or xylene) and the methacrylate monomer dissolves the polycarbonate. Does anyone have any experience trying process such a thing, an embedding media (pmma, OCT for cryo, etc.), and then a method of sectioning it keeping the interface intact? Thanks in advance for any suggestions! John John A. Baker The University of Michigan Orthopaedic Research Laboratories Histology Unit 109 Zina Pitcher Place, 2218 BSRB Ann Arbor, MI 48109-2200 734-936-1635 ********************************************************** Electronic Mail is not secure, may not be read every day, and should not be used for urgent or sensitive issues _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Feb 7 09:36:41 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Feb 7 09:36:44 2013 Subject: [Histonet] Xylene/Paint Thinner In-Reply-To: <2B2ECF33934F5D4996D8BE03EFDF39760AE21D91@VHAV09MSGA3.v09.med.va.gov> References: <2B2ECF33934F5D4996D8BE03EFDF39760AE21D91@VHAV09MSGA3.v09.med.va.gov> Message-ID: <1360251401.83478.YahooMailNeo@web163105.mail.bf1.yahoo.com> For sure they do not know, I have asked a few painters. As many histotechs, they have told me that the "like the smell of xylene". Ren? J. From: "White, Lisa M." To: histonet@lists.utsouthwestern.edu Sent: Thursday, February 7, 2013 10:23 AM Subject: [Histonet] Xylene/Paint Thinner If you look on the paint thinner isle at your local Lowe's.? You will find Xylene on the shelf.? The first time I saw it was a shock.? Wonder if the home improvement? weekend warriors know what they are getting all over their hands????? Lisa White, HT(ASCP) Supervisory HT James H. Quillen VAMC PO Box 4000 Corner of Veterans Way and Lamont PLMS 113 Mountain Home, TN 37684 423-979-3567 423-979-3401 fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rennie1108 <@t> yahoo.com Thu Feb 7 09:49:22 2013 From: rennie1108 <@t> yahoo.com (Adrienne Anderson) Date: Thu Feb 7 09:49:35 2013 Subject: [Histonet] Xylene/Paint Thinner In-Reply-To: <1360251401.83478.YahooMailNeo@web163105.mail.bf1.yahoo.com> References: <2B2ECF33934F5D4996D8BE03EFDF39760AE21D91@VHAV09MSGA3.v09.med.va.gov> <1360251401.83478.YahooMailNeo@web163105.mail.bf1.yahoo.com> Message-ID: <4ACB68F3-A680-4769-89DC-24FA8F196687@yahoo.com> I have to admit, I don't mind the smell:) But I also like the smell of gasoline, and I know both are bad for me! Thanks again to everyone who has offered feedback on this topic. Rene, I have printed out a few of the articles you've written and am anxious to research these methods more. Thanks a bunch! Best, Adrienne On Feb 7, 2013, at 10:36 AM, Rene J Buesa wrote: > For sure they do not know, I have asked a few painters. As many histotechs, they have told me that the "like the smell of xylene". > Ren? J. > > From: "White, Lisa M." > To: histonet@lists.utsouthwestern.edu > Sent: Thursday, February 7, 2013 10:23 AM > Subject: [Histonet] Xylene/Paint Thinner > > If you look on the paint thinner isle at your local Lowe's. You will > find Xylene on the shelf. The first time I saw it was a shock. Wonder > if the home improvement weekend warriors know what they are getting all > over their hands????? > > > > Lisa White, HT(ASCP) > > Supervisory HT > > James H. Quillen VAMC > > PO Box 4000 > > Corner of Veterans Way and Lamont > > PLMS 113 > > Mountain Home, TN 37684 > > 423-979-3567 > > 423-979-3401 fax > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vtobias <@t> uw.edu Thu Feb 7 10:06:56 2013 From: vtobias <@t> uw.edu (Victor A. Tobias) Date: Thu Feb 7 10:07:22 2013 Subject: [Histonet] RE: Xylene/Paint Thinner In-Reply-To: <2B2ECF33934F5D4996D8BE03EFDF39760AE21D91@VHAV09MSGA3.v09.med.va.gov> References: <2B2ECF33934F5D4996D8BE03EFDF39760AE21D91@VHAV09MSGA3.v09.med.va.gov> Message-ID: I don't know if the auto parts stores carry xylene, but it is an awesome degreaser. Learned that from my mentor back in the 70's. Victor Victor Tobias HT(ASCP) Clinical Applications Analyst Harborview Medical Center Dept of Pathology Room NJB244 Seattle, WA 98104 vtobias@u.washington.edu 206-744-2735 206-744-8240 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of White, Lisa M. Sent: Thursday, February 07, 2013 7:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Xylene/Paint Thinner If you look on the paint thinner isle at your local Lowe's. You will find Xylene on the shelf. The first time I saw it was a shock. Wonder if the home improvement weekend warriors know what they are getting all over their hands????? Lisa White, HT(ASCP) Supervisory HT James H. Quillen VAMC PO Box 4000 Corner of Veterans Way and Lamont PLMS 113 Mountain Home, TN 37684 423-979-3567 423-979-3401 fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Feb 7 10:13:32 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Feb 7 10:13:41 2013 Subject: [Histonet] RE: Xylene/Paint Thinner In-Reply-To: References: <2B2ECF33934F5D4996D8BE03EFDF39760AE21D91@VHAV09MSGA3.v09.med.va.gov> Message-ID: <1360253612.31245.YahooMailNeo@web163103.mail.bf1.yahoo.com> If you ever need to degrease any auto part, just place it in water, add?liquid?soap?to about 5% conc., and heat it until boiling and let them in boiling water during 5 minutes. Wash with running tap water and dry. They will degrease even better tan with xylene. Ren? J. From: Victor A. Tobias To: "'White, Lisa M.'" ; "'histonet@lists.utsouthwestern.edu'" Sent: Thursday, February 7, 2013 11:06 AM Subject: [Histonet] RE: Xylene/Paint Thinner I don't know if the auto parts stores carry xylene, but it is an awesome degreaser. Learned that from my mentor back in the 70's. Victor Victor Tobias HT(ASCP) Clinical Applications Analyst Harborview Medical Center Dept of Pathology Room NJB244 Seattle, WA 98104 vtobias@u.washington.edu 206-744-2735 206-744-8240 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of White, Lisa M. Sent: Thursday, February 07, 2013 7:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Xylene/Paint Thinner If you look on the paint thinner isle at your local Lowe's.? You will find Xylene on the shelf.? The first time I saw it was a shock.? Wonder if the home improvement? weekend warriors know what they are getting all over their hands????? Lisa White, HT(ASCP) Supervisory HT James H. Quillen VAMC PO Box 4000 Corner of Veterans Way and Lamont PLMS 113 Mountain Home, TN 37684 423-979-3567 423-979-3401 fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vtobias <@t> uw.edu Thu Feb 7 10:17:07 2013 From: vtobias <@t> uw.edu (Victor A. Tobias) Date: Thu Feb 7 10:17:33 2013 Subject: [Histonet] RE: Xylene/Paint Thinner In-Reply-To: <1360253612.31245.YahooMailNeo@web163103.mail.bf1.yahoo.com> References: <2B2ECF33934F5D4996D8BE03EFDF39760AE21D91@VHAV09MSGA3.v09.med.va.gov> <1360253612.31245.YahooMailNeo@web163103.mail.bf1.yahoo.com> Message-ID: I'm sure my wife would love that. Victor Tobias HT(ASCP) Clinical Applications Analyst Harborview Medical Center Dept of Pathology Room NJB244 Seattle, WA 98104 vtobias@u.washington.edu 206-744-2735 206-744-8240 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. ________________________________ From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: Thursday, February 07, 2013 8:14 AM To: Victor A. Tobias; 'White, Lisa M.'; 'histonet@lists.utsouthwestern.edu' Subject: Re: [Histonet] RE: Xylene/Paint Thinner If you ever need to degrease any auto part, just place it in water, add liquid soap to about 5% conc., and heat it until boiling and let them in boiling water during 5 minutes. Wash with running tap water and dry. They will degrease even better tan with xylene. Ren? J. From: Victor A. Tobias To: "'White, Lisa M.'" ; "'histonet@lists.utsouthwestern.edu'" Sent: Thursday, February 7, 2013 11:06 AM Subject: [Histonet] RE: Xylene/Paint Thinner I don't know if the auto parts stores carry xylene, but it is an awesome degreaser. Learned that from my mentor back in the 70's. Victor Victor Tobias HT(ASCP) Clinical Applications Analyst Harborview Medical Center Dept of Pathology Room NJB244 Seattle, WA 98104 vtobias@u.washington.edu 206-744-2735 206-744-8240 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of White, Lisa M. Sent: Thursday, February 07, 2013 7:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Xylene/Paint Thinner If you look on the paint thinner isle at your local Lowe's. You will find Xylene on the shelf. The first time I saw it was a shock. Wonder if the home improvement weekend warriors know what they are getting all over their hands????? Lisa White, HT(ASCP) Supervisory HT James H. Quillen VAMC PO Box 4000 Corner of Veterans Way and Lamont PLMS 113 Mountain Home, TN 37684 423-979-3567 423-979-3401 fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jeremiah.preszler <@t> us.af.mil Thu Feb 7 10:20:59 2013 From: jeremiah.preszler <@t> us.af.mil (PRESZLER, JEREMIAH C MSgt USAF AETC 59 LSQ/SGVLH) Date: Thu Feb 7 10:21:20 2013 Subject: [Histonet] FW: Processor dehydration cycles.. Message-ID: <535971279F56F9458E646CB1B2AA53C50696124248@52VEJX-MV15-01.area52.afnoapps.usaf.mil> I agree with Joyce on this: Formalin salt precipitate tends to become more common if you start above 70%. WE use a 70%, then 80% and two 95% in our process here. Very Respectfully, Jeremiah C. Preszler, MSgt, USAF HT (ASCP) Flight Chief, Anatomic Pathology 959 CSPS/ SGVLH WHASC JBSA-Lackland AFB, TX 78236 (210) 292-5519 DSN: 662-5519 From Joyce.Weems <@t> emoryhealthcare.org Thu Feb 7 10:23:59 2013 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Thu Feb 7 10:24:48 2013 Subject: [Histonet] RE: Xylene/Paint Thinner In-Reply-To: <1360253612.31245.YahooMailNeo@web163103.mail.bf1.yahoo.com> References: <2B2ECF33934F5D4996D8BE03EFDF39760AE21D91@VHAV09MSGA3.v09.med.va.gov> <1360253612.31245.YahooMailNeo@web163103.mail.bf1.yahoo.com> Message-ID: And Dawn is the best... And I am in no way linked to this product!! Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, February 07, 2013 11:14 AM To: Victor A. Tobias; 'White, Lisa M.'; 'histonet@lists.utsouthwestern.edu' Subject: Re: [Histonet] RE: Xylene/Paint Thinner If you ever need to degrease any auto part, just place it in water, add liquid soap to about 5% conc., and heat it until boiling and let them in boiling water during 5 minutes. Wash with running tap water and dry. They will degrease even better tan with xylene. Ren? J. From: Victor A. Tobias To: "'White, Lisa M.'" ; "'histonet@lists.utsouthwestern.edu'" Sent: Thursday, February 7, 2013 11:06 AM Subject: [Histonet] RE: Xylene/Paint Thinner I don't know if the auto parts stores carry xylene, but it is an awesome degreaser. Learned that from my mentor back in the 70's. Victor Victor Tobias HT(ASCP) Clinical Applications Analyst Harborview Medical Center Dept of Pathology Room NJB244 Seattle, WA 98104 vtobias@u.washington.edu 206-744-2735 206-744-8240 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of White, Lisa M. Sent: Thursday, February 07, 2013 7:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Xylene/Paint Thinner If you look on the paint thinner isle at your local Lowe's. You will find Xylene on the shelf. The first time I saw it was a shock. Wonder if the home improvement weekend warriors know what they are getting all over their hands????? Lisa White, HT(ASCP) Supervisory HT James H. Quillen VAMC PO Box 4000 Corner of Veterans Way and Lamont PLMS 113 Mountain Home, TN 37684 423-979-3567 423-979-3401 fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From Timothy.Morken <@t> ucsfmedctr.org Thu Feb 7 10:51:19 2013 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Thu Feb 7 10:51:33 2013 Subject: [Histonet] RE: Processor dehydration cycles.. In-Reply-To: References: Message-ID: <761E2B5697F795489C8710BCC72141FF054381@ex07.net.ucsf.edu> Tom, We start at 80%. Tim Morken UCSF Pathology -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Thursday, February 07, 2013 7:15 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Processor dehydration cycles.. Hello all, I was wondering what most people use as the first reagent after the formalins on their tissue processor? We have always used a sequence of 70%, 80%, 95%, and 100% but is anyone using 80% or even 95% to start their dehydration? Thanks in advance. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org ________________________________ This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From marktarango <@t> gmail.com Thu Feb 7 10:55:25 2013 From: marktarango <@t> gmail.com (Mark Tarango) Date: Thu Feb 7 10:55:30 2013 Subject: [Histonet] High complexity test In-Reply-To: <1360250554.2421.YahooMailNeo@web163101.mail.bf1.yahoo.com> References: <1360184654.37455.YahooMailNeo@web163105.mail.bf1.yahoo.com> <8191357C-067A-4E2C-8471-59EE8D59D774@yumaregional.org> <761E2B5697F795489C8710BCC72141FF0541FD@ex07.net.ucsf.edu> <1360250554.2421.YahooMailNeo@web163101.mail.bf1.yahoo.com> Message-ID: FISH is definitely high complexity. The tech who scores the slide must meet the qualifications to perform high complexity testing. I understood Kathy's question to be about the person who loads and runs the slide stainer. I still say give CAP or whomever you accrediting agency is a call and see what they say. Histonet advice is just that. Mark On Thu, Feb 7, 2013 at 7:22 AM, Rene J Buesa wrote: > I only hope that Tim's posting sets this issue to rest. The histotech > doing IHC, FISH, or grossing and some other complex tasks has to have > special training and studies because all those are high complexity tests. > Even those histotechs reading FISH results (counting the reactive nuclei > for latter signing by the pathologist) have to receive special training. > I will just point out again that some administrators try to "underrate" > these tests in order to train "their monkeys" and pay less even when the > ratio billing/cost is extremely high. > Management greed drives sometimes these issues. > Ren? J. > > From: "Morken, Timothy" > To: "histonet@lists.utsouthwestern.edu" > > Sent: Wednesday, February 6, 2013 5:47 PM > Subject: RE: [Histonet] High complexity test > > The CLIA definition of High Complexity testing is not absolute, rather > High Complexity Testing is determined by a scored algorithm of the entire > "Test System" (preanalytical through Post Analytical). > > As such, it clearly takes into account the laboratory or other personnel > performing all the specimen collection, grossing, processing, cutting in > the pre-analytical phase, and the "Testing Personnel" (CLIA Definition) > performing the analytical phase (preparing slides, reagents, applying > reagents, quality control, etc) and post analytical phase(interpretation) . > > The Pathologist's role is only part of that and is scored accordingly. > CLIA clearly considers the IHC Test System as High Complexity and requires > a technologist for the IHC portion with at least and Associates degree (or > equivalent, including course and experience in appropriate science and > testing) for Testing Personnel. > > So, I don't think it is correct to dismiss any personnel standards as > irrelevant simply because a pathologist will do the interpretation. > > Note that if ANY High Complexity tests are performed in the lab then the > lab must have a CLIA certification for High Complexity Testing. > > > References: > > CLIA website with the personnel regulations: > http://wwwn.cdc.gov/clia/regs/subpart_m.aspx > > CAP Q&A about personnel standards for IHC, ISH and IF > http://www.cap.org/apps/docs/education/lapaudio/pdf/031710_qa.pdf > > Relevant IHC question reproduced below: > Q 17. My question refers more specifically to > immunofluorescence, in situ > hybridization and > immunohistochemistry. Are the techs > that perform these tests considered > high complexity testing personnel? If > the techs are reporting any kind of > preliminary result, they must be > qualified to do high complexity testing. > If all they are doing is applying the > stain, then that is considered > processing. > A: Personnel performing immunofluorescence, > immunohistochemistry and in-situ hybridization > techniques require qualifications applicable to high > complexity testing. Personnel performing histology > processing using routine standardized staining > procedures (not classified as molecular) do not fall > under CLIA as testing personnel and do not have > qualification requirements define > > > CLIA website detailing test categorization: > http://wwwn.cdc.gov/clia/regs/subpart_a.aspx#493.17 > > Excerpt here about High vs Moderate complexity (low complexity are > basically home use tests) > > Sec. 493.17 Test categorization. > > (a) Categorization by criteria. Notices will be published in the > Federal Register which list each specific test system, assay, and > examination categorized by complexity. Using the seven criteria > specified in this paragraph for categorizing tests of moderate or > high > complexity, each specific laboratory test system, assay, and > examination > will be graded for level of complexity by assigning scores of 1, 2, > or 3 > within each criteria. The score of "1" indicates the lowest level of > complexity, and the score of "3" indicates the highest level. These > scores will be totaled. Test systems, assays or examinations > receiving > scores of 12 or less will be categorized as moderate complexity, > while > those receiving scores above 12 will be categorized as high > complexity. > > Note: A score of "2" will be assigned to a criteria heading when > the characteristics for a particular test are intermediate between > the > descriptions listed for scores of "1" and "3." > > (1) Knowledge. > (i) Score 1. (A) Minimal scientific and technical knowledge is > required to perform the test; and > (B) Knowledge required to perform the test may be obtained through > on-the-job instruction. > (ii) Score 3. Specialized scientific and technical knowledge is > essential to perform preanalytic, analytic or postanalytic phases of > the > testing. > (2) Training and experience. > (i) Score 1. (A) Minimal training is required for preanalytic, > analytic and postanalytic phases of the testing process; and > (B) Limited experience is required to perform the test. > (ii) Score 3. (A) Specialized training is essential to perform the > preanalytic, analytic or postanalytic testing process; or > (B) Substantial experience may be necessary for analytic test > performance. > (3) Reagents and materials preparation. > (i) Score 1. (A) Reagents and materials are generally stable and > reliable; and > (B) Reagents and materials are prepackaged, or premeasured, or > Require no special handling, precautions or storage conditions. > (ii) Score 3. (A) Reagents and materials may be labile and may > require special handling to assure reliability; or > (B) Reagents and materials preparation may include manual steps such > as gravimetric or volumetric measurements. > (4) Characteristics of operational steps. (i) Score 1. Operational > steps are either automatically executed (such as pipetting, > temperature > monitoring, or timing of steps), or are easily controlled. > (ii) Score 3. Operational steps in the testing process require close > monitoring or control, and may require special specimen preparation, > precise temperature control or timing of procedural steps, accurate > pipetting, or extensive calculations. > (5) Calibration, quality control, and proficiency testing materials. > (i) Score 1. (A) Calibration materials are stable and readily > available; > (B) Quality control materials are stable and readily available; and > (C) External proficiency testing materials, when available, are > stable. > (ii) Score 3. (A) Calibration materials, if available, may be > labile; > (B) Quality control materials may be labile, or not available; or > (C) External proficiency testing materials, if available, may be > labile. > (6) Test system troubleshooting and equipment maintenance. > (i) Score 1. (A) Test system troubleshooting is automatic or self- > correcting, or clearly described or requires minimal judgment; and > (B) Equipment maintenance is provided by the manufacturer, is seldom > needed, or can easily be performed. > (ii) Score 3. (A) Troubleshooting is not automatic and requires > decision-making and direct intervention to resolve most problems; or > (B) Maintenance requires special knowledge, skills, and abilities. > (7) Interpretation and judgment. (i) Score 1. (A) Minimal > interpretation and judgment are required to perform preanalytic, > analytic and postanalytic processes; and > (B) Resolution of problems requires limited independent > interpretation and judgment; and > (ii) Score 3. (A) Extensive independent interpretation and judgment > are required to perform the preanalytic, analytic or postanalytic > processes; and > (B) Resolution of problems requires extensive interpretation and > judgment. > > > Tim Morken > Supervisor, Electron Microscopy/Neuromuscular Special Studies > Department of Pathology > UC San Francisco Medical Center > > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Tarango > Sent: Wednesday, February 06, 2013 2:07 PM > To: Jesus Ellin > Cc: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] High complexity test > > Just to clarify, this is not my interpretation. This is what CAP will > tell you when you give them a call. > > Mark > > On Wed, Feb 6, 2013 at 1:07 PM, Jesus Ellin > wrote: > > > I would say this is high complexoty testing and the tech performing > > this has to have knowledge of the process and troubleshooting in case > > there is issues with the results. I do not agree with the > > interpretation some people give,, but this is based on individual > > institutions > > > > Sent from my iPad > > > > On Feb 6, 2013, at 2:05 PM, "Rene J Buesa" wrote: > > > > > This issue has been discussed at length recently (please go to > > > HistoNet > > files). > > > The "complexity" does not deals with the "actual test" but with the > > ability of the technician to go above and beyond the "robotic tasks" > > but also able to think and apply knowledge when something goes wrong. > > > Sometimes dismissal of complexity is rooted on the desire in > > > management > > to pay less for tasks that require a higher licensure grade. > > > Ren? J. > > > > > > From: Sara Baldwin/mhhcc.org > > > To: histonet@lists.utsouthwestern.edu > > > Sent: Wednesday, February 6, 2013 2:54 PM > > > Subject: [Histonet] High complexity test > > > > > > Hi histonetters > > > Is ventana Ultra IHC only doing antibodies no FISH or CISH is this > > considered High complexity testing? We are doing ER/PR and some others. > > > > > > Thanks > > > Histology/Cytology Supervisor > > > S. Kathy Baldwin, SCT (ASCP) > > > Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph > > > 812-996-0210, 0216, Fax 812-996-0232, Pager 812-481-0897, Cell > > > 812-887-3357 _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ______________________________________________________________________ > > This message is confidential, intended only for the named > > recipient(s) and may contain information that is privileged or exempt > > from disclosure under applicable law. If you are not the intended > > recipient(s), you are notified that the dissemination, distribution, > > or copying of this message is strictly prohibited. If you receive > > this message in error, or are not the named recipient(s), please > > notify the sender at either the e-mail, fax, address, or telephone > > number listed above and delete this e-mail from your computer. > > Thank You. > > ______________________________________________________________________ > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From twheelock <@t> mclean.harvard.edu Thu Feb 7 10:56:37 2013 From: twheelock <@t> mclean.harvard.edu (Tim Wheelock) Date: Thu Feb 7 10:56:40 2013 Subject: [Histonet] Processor dehydration cycles Message-ID: <5113DCC5.5020102@mclean.harvard.edu> Hi Tom: I deal exclusively with post-mortum brain tissue, so my situation may not apply to you. I do not use formalin on my processor, since the half brain used for brain-cutting has already been thoroughly fixed. So, I have the luxury of using 30%, 50%, 80%, 95%, then three 100% Isopropanols. They used this protocol when I was at a different neuropathology laboratory. I believe the rational for this was that starting with a lower concentration of alcohol, and then more gradually increasing the concentrations, would reduce the concentration gradient between the cells and the solution, and so avoid strong currents from harming the cells. Also, brain tissue may be more delicate that say prostate, skin or uterus. By the way, I actually do not know whether this "rational" is correct or not. However, in general, if you can start at 70%, it can't do any harm. Tim Tim Wheelock Harvard Brain Bank McLean Hospital Belmont, MA (617) 855-359 Tom McNemar wrote: > Hello all, > > I was wondering what most people use as the first reagent after the formalins on their tissue processor? We have always used a sequence of 70%, 80%, 95%, and 100% but is anyone using 80% or even 95% to start their dehydration? > > Thanks in advance. > > Tom McNemar, HT(ASCP) > Histology Co-ordinator > Licking Memorial Health Systems > (740) 348-4163 > (740) 348-4166 > tmcnemar@lmhealth.org > www.LMHealth.org From JMaslanka <@t> stpetes.org Thu Feb 7 11:28:37 2013 From: JMaslanka <@t> stpetes.org (JMaslanka@stpetes.org) Date: Thu Feb 7 11:29:26 2013 Subject: [Histonet] Disposal of old blocks & Slides Message-ID: How do you dispose of old tissue blocks after the 10 year hold period? Can old glass tissue slides be sent for recycling? Thanks in advance Joe Maslanka BS, CT,HT (ASCP) Anatomical Pathology Technical Supervisor St Peter's Hospital,MT 59601 (P)(406) 447-2406 (F)(406)444-2126 Give thanks for ALL things..... "Kindness is the language the blind can see & the deaf can hear- Mark Twain From rheyna <@t> lumc.edu Thu Feb 7 11:45:44 2013 From: rheyna <@t> lumc.edu (Roger Heyna) Date: Thu Feb 7 11:45:59 2013 Subject: [Histonet] Re: Leica Bond III Opinions In-Reply-To: References: Message-ID: <511393E80200002300049DF0@gwgwia1.luhs.org> Forwarding a message I received: Hi Roger, I've been using the Lieca Bonds for about 4 years now and I wouldn't trade for anything. The lab I am currently at changed everything from Ventana over to Bonds about a year and a half ago, and we have NO regrets! Bonds are faster, more flexible with protocols, and have been producing higher quality staining. The test is also cheaper on Bonds than on Ventana. For me, I think the flexibility with protocols and the reliable, consistent, good staining is the real bonus. Your questions: Are you happy with the quality of the stains you're performing on the Bond III? - ABSOLUTELY, NO REGRETS Are there antibodies that you were not able to run on the Bond III? - NONE SO FAR. I USE ANTIBODIES FROM VARIOUS SUPPLIERS, I DON'T LIMIT MYSELF TO JUST LEICA ANTIBODIES Is the machine reliable, or does it break down often, requiring technical service? - RARELY NEEDS TECHNICAL SERVICE, I DO NOTE THAT KEEPING THE SLIDE ASSEMBLY PADS CLEAN (ESPECIALLY THE DRAIN HOLES) IS IMPORTANT FOR OPTIMAL STAINING. WE DO A GOOD CLEANING MONTHLY How arduous is cleaning and managing the cover tiles? SIMPLE ENOUGH. USED TILES ARE SLID INTO A RACK SITTING IN DISTILLED WATER AFTER USE. WHEN THE RACK IS FILLED, WE ADD SOME BLEACH, LET SIT FOR 20 - 3O MINUTES, RINSE THE WHOLE RACK WELL IN DISTILLED, RINSE IN ABSOLUTE ALCOHOL, SIT OUT TO DRY . Are the three black slide drawers durable? I'VE NEVER HAD ONE BREAK OR WARP. Anything else I can answer? Beth Cox, HTL/SCT(ASCP)QIHC Message: 7 Date: Wed, 06 Feb 2013 13:15:35 -0600 From: "Roger Heyna" Subject: [Histonet] Leica Bond III Opinions To: Message-ID: <511257770200002300049CAA@gwgwia1.luhs.org> Content-Type: text/plain; charset="us-ascii" Could I get the opinions of any labs using the Leica Bond III for IHC staining? I'm especially interested to hear from those that switched from Ventana to Leica, but any feedback is appreciated. Few questions that come to mind: Are you happy with the quality of the stains you're performing on the Bond III? Are there antibodies that you were not able to run on the Bond III? Is the machine reliable, or does it break down often, requiring technical service? How arduous is cleaning and managing the cover tiles? Are the three black slide drawers durable? Thank you, Roger Heyna Maywood, IL From HParker <@t> Skaggs.Net Thu Feb 7 12:34:16 2013 From: HParker <@t> Skaggs.Net (Parker, Helayne) Date: Thu Feb 7 12:34:22 2013 Subject: [Histonet] Processor dehydration cycles In-Reply-To: References: Message-ID: <930EB2E8DF68C544873EDD2A3D5F506007D0300CFB@email1.skaggs.net> -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Hello all, <<>>> We use start at 95%, but we have two Pen Fix (alcohol fixative) steps between our Formalin and alcohols. 10% NBF, 10% NBF, PenFix, PenFix and then the alcohols starting at 95%. Speaking of 10% NBF has anyone ever used un-buffered formalin for routine processing? I heard of a place using un-buffered formalin and wondered if 1) would that be okay or is it harsh on tissue and 2) would it keep the salt deposits down? Helayne Parker, H.T. (ASCP) Pathology Section Head Cox Medical Center Branson P.O. Box 650, Branson, MO 65615 Phone: 417-335-7254 Fax: 417-335-7127 Email: hparker@skaggs.net Web: www.coxhealth.com/branson -------------- next part -------------- CoxHealth ? ranked one of Missouri's Best Hospitals by U.S. News & World Report COXHEALTH CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From Kim.Kolman <@t> va.gov Thu Feb 7 12:41:06 2013 From: Kim.Kolman <@t> va.gov (Kolman, Kimberly D.) Date: Thu Feb 7 12:41:28 2013 Subject: [Histonet] cryostat decontamination Message-ID: <9C32F30B6662D74A8419DDDB7E66656A07EB992B@VHAV15MSGA1.v15.med.va.gov> Has anyone come up with a documented and referenced procedure for decontamination of a cryostat using 37% Formaldehyde? I pulled up old notes in the Histonet archives and saw that Tim Morken was working on this but that was a number of years ago..... Thanks, Kim Kimberly D. Kolman, HT, (ASCP) Diagnostics 115 VA Eastern Kansas Health Care System 4101 S. 4th St. Trfwy. Leavenworth, KS 66048 ph: 913-682-2000 x 52537/52539 From Steven.Swartwood <@t> cshs.org Thu Feb 7 13:33:53 2013 From: Steven.Swartwood <@t> cshs.org (Swartwood, Steven J) Date: Thu Feb 7 13:33:58 2013 Subject: [Histonet] Double staining with IF/IHC - chromogenic Message-ID: <959202AC61AEF942968646EC66E2BE3E4058A5@CSHSMSGMBX05.CSMC.EDU> Hello everyone, I have a co-worker in the research department that would like to know if anyone has ever combined and IF stain with chromogenic IHC staining. His theory for this is that IF shows better nuclear staining from an image analysis stand point. If you have heard of this can you send me literature on it, or if you've done this what is your protocol? It would seem that doing IF first than IHC might cause quenching of the IF, but then again doing chromogenic staining first might cover the IF antigen. I suspect that only an chromogenic cytoplasmic IHC stain can be combined with a nuclear IF marker with IHC first. Any ideas? Again this is specifically from an image analysis stand point. Steven Swartwood HT(ASCP) Cedars Sinai Medical Center steven.swartwood@cshs.org IMPORTANT WARNING: This message is intended for the use of the person or entity to which it is addressed and may contain information that is privileged and confidential, the disclosure of which is governed by applicable law. If the reader of this message is not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this information is STRICTLY PROHIBITED. If you have received this message in error, please notify us immediately by calling (310) 423-6428 and destroy the related message. Thank You for your cooperation. From jeremiah.preszler <@t> us.af.mil Thu Feb 7 13:38:12 2013 From: jeremiah.preszler <@t> us.af.mil (PRESZLER, JEREMIAH C MSgt USAF AETC 59 LSQ/SGVLH) Date: Thu Feb 7 13:38:29 2013 Subject: [Histonet] Disposal of old blocks & Slides Message-ID: <535971279F56F9458E646CB1B2AA53C506961243E7@52VEJX-MV15-01.area52.afnoapps.usaf.mil> Joe, We "redbag" all paraffin blocks for medical waste disposal. We use large sharps containers for disposal of glass slides. Very Respectfully, Jeremiah C. Preszler, MSgt, USAF HT (ASCP) Flight Chief, Anatomic Pathology 959 CSPS/ SGVLH WHASC JBSA-Lackland AFB, TX 78236 (210) 292-5519 DSN: 662-5519 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of JMaslanka@stpetes.org Sent: Thursday, February 07, 2013 11:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Disposal of old blocks & Slides How do you dispose of old tissue blocks after the 10 year hold period? Can old glass tissue slides be sent for recycling? Thanks in advance Joe Maslanka BS, CT,HT (ASCP) Anatomical Pathology Technical Supervisor St Peter's Hospital,MT 59601 (P)(406) 447-2406 (F)(406)444-2126 Give thanks for ALL things..... "Kindness is the language the blind can see & the deaf can hear- Mark Twain From jsjurczak <@t> comcast.net Thu Feb 7 14:40:25 2013 From: jsjurczak <@t> comcast.net (jsjurczak@comcast.net) Date: Thu Feb 7 14:40:50 2013 Subject: [Histonet] PIN4 staining Message-ID: <1989017542.863749.1360269625850.JavaMail.root@sz0094a.emeryville.ca.mail.comcast.net> Anybody else having problems with PIN 4 staining (Biocare)? We are getting brown background staining, even on the glass itself where there is no tissue. After many years of fantastic results, this problem has occurred recently. Our AMACR is also a lot fainter than it used to be. We've switched out all lots of reagents involved, even bought distilled water. Still no movement towards solving this problem. Any ideas? Jeff Jurczak Uropartners Laboratory Westchester, IL From becway <@t> yahoo.com Thu Feb 7 15:13:50 2013 From: becway <@t> yahoo.com (Becky Orr) Date: Thu Feb 7 15:14:00 2013 Subject: [Histonet] =?windows-1252?q?Re=3A_Histonet_Digest=2C_Vol_111=2C_?= =?windows-1252?q?Iss=E6ue_8?= Message-ID: <593672815-1360271635-cardhu_decombobulator_blackberry.rim.net-1471415453-@b4.c17.bise6.blackberry> Sent from my Verizon Wireless BlackBerry -----Original Message----- From: histonet-request@lists.utsouthwestern.edu Sender: histonet-bounces@lists.utsouthwestern.edu To: Reply-To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 111, Issue 8 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: Xylene Substitutes (Blazek, Linda) 2. Graveyard Histotech Position in Spokane, Washington (Miranda Giorgi) 3. RE: Histonet Digest, Vol 111, Issue 7 (Parker, Helayne) ---------------------------------------------------------------------- Message: 1 Date: Wed, 6 Feb 2013 12:13:40 -0500 From: "Blazek, Linda" Subject: RE: [Histonet] Xylene Substitutes To: Adrienne Anderson , "histonet@lists.utsouthwestern.edu" Message-ID: <5A2BD13465E061429D6455C8D6B40E3916490A9AAA@IBMB7Exchange.digestivespecialists.com> Content-Type: text/plain; charset="us-ascii" We have used Formula 83 from CBG for years with excellent results. We use it for processing and staining. We also recycle it making the cost very low. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dessoye, Michael J Sent: Wednesday, February 06, 2013 11:47 AM To: Adrienne Anderson; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Xylene Substitutes We used SubX but have switched to Clear-Rite 3 from Thermo Fisher with excellent results as well as cost savings. Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdessoye@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 -----Original Message----- From: Adrienne Anderson [mailto:rennie1108@yahoo.com] Sent: Tuesday, February 05, 2013 4:58 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Xylene Substitutes Hello all, My lab is looking into xylene substitutes, and I'd love some feedback on what other labs are using. We currently use SubX, but are there other items out there more economical? Thanks, Adrienne _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Commonwealth Health. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Wed, 6 Feb 2013 17:18:22 +0000 From: Miranda Giorgi Subject: [Histonet] Graveyard Histotech Position in Spokane, Washington To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <46BB82E6FC36E44FB454E00C82DBDC56CD1A80@icpmail.PAI.E-PATHOLOGY.com> Content-Type: text/plain; charset="us-ascii" Hello All, Please see the job posting below for a Histotech position at InCyte Pathology in Spokane, WA. Only interested candidates should reply. Please do not respond if you are from a recruitment service. Thank you! Histology Analyst, Graveyard Shift (M-F) InCyte Pathology is a full-service, pathologist-owned anatomic pathology laboratory. Our one-hundred employees and twenty-one board-certified pathologists provide exceptional laboratory services to hospitals and physician offices throughout the Pacific Northwest, Montana, and Alaska from our state-of-the-art facility located in Spokane Valley, Washington. We are just a short drive from five major ski resorts, several beautiful lakes, and a variety of outdoor activities. Qualifications: HT certified or HTL registry eligible, and two years of laboratory experience. This position performs all routine procedures in orienting, sectioning, and staining tissue specimens to produce slides which will aide pathologists in diagnosis. Preference will be given to those candidates who are IHC qualified and have proven leadership qualities. We offer competitive salaries and an outstanding benefits package including medical, dental, life and disability insurance, 401(k), and a liberal paid time off program. Relocation assistance is available. Please email your cover letter and resume to humanresources@incytepathology.com. Please reference where you saw this ad. No phone calls or solicitors! Miranda Giorgi, HTL (ASCP)CM Histology Supervisor InCyte Pathology This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the InCyte Privacy Officer at privacy@icplab.com or call (509) 892-2700. ------------------------------ Message: 3 Date: Wed, 6 Feb 2013 11:49:04 -0600 From: "Parker, Helayne" Subject: [Histonet] RE: Histonet Digest, Vol 111, Issue 7 To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <930EB2E8DF68C544873EDD2A3D5F506007D0300CF5@email1.skaggs.net> Content-Type: text/plain; charset="us-ascii" <<<>>> We use Clear-Rite 3 made by Richard Allan and is purchased at Cardinal. I do not think it is very expensive we buy in a case of 4 gallons. It does a decent job for clearing in the processor and de-paraffinizing slides, but we do coverslip out of xylene at the end of the stain line. Actually we never tried to coverslip out of it Clear-Rite 3 so can not honestly give you a critique in ref to that. We keep both on hand. Xylene for machine cleaning and coverslipping and Clear-Rite 3 for processing and de-paraffinizing. I like it better than Americlear and ProPar. http://www.cardinalhealth.com/us/en/distributedproducts/ASP/C4405-20.asp?cat=physician Helayne Parker, H.T. (ASCP) Pathology Section Head Cox Medical Center Branson P.O. Box 650, Branson, MO 65615 Phone: 417-335-7254 Fax: 417-335-7127 Email: hparker@skaggs.net Web: www.coxhealth.com/branson -------------- next part -------------- CoxHealth ? ranked one of Missouri's Best Hospitals by U.S. News & World Report COXHEALTH CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 111, Issue 8 **************************************** From robin_dean <@t> compbio.com Thu Feb 7 18:49:54 2013 From: robin_dean <@t> compbio.com (Robin Dean) Date: Thu Feb 7 18:51:50 2013 Subject: [Histonet] best fixative for eyes Message-ID: <007601ce0596$35cddc00$a1699400$@com> What are people's thoughts/experience with different fixatives for eyes (rat, mouse and rabbit mostly). We currently use modified Davidson's solution, but the pathologist is considering going to Davidson's. I guess there has been some difficulty with cutting due to the lens not getting fixed well and causing problems. Thank you for sharing From b427297 <@t> aol.com Thu Feb 7 21:16:48 2013 From: b427297 <@t> aol.com (Jackie O'Connor) Date: Thu Feb 7 21:16:53 2013 Subject: [Histonet] best fixative for eyes In-Reply-To: <007601ce0596$35cddc00$a1699400$@com> References: <007601ce0596$35cddc00$a1699400$@com> Message-ID: <8CFD3C1DDA33EC5-1328-210FF@webmail-m164.sysops.aol.com> How long are you leaving the eyes in the mDavidsons? We have found 24 hours works extremely well. -----Original Message----- From: Robin Dean To: histonet Sent: Thu, Feb 7, 2013 6:52 pm Subject: [Histonet] best fixative for eyes What are people's thoughts/experience with different fixatives for eyes (rat, mouse and rabbit mostly). We currently use modified Davidson's solution, but the pathologist is considering going to Davidson's. I guess there has been some difficulty with cutting due to the lens not getting fixed well and causing problems. Thank you for sharing _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Wed Feb 6 20:56:04 2013 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Thu Feb 7 23:30:57 2013 Subject: [Histonet] Leica Bond III Opinions Message-ID: <02w02n0ntoljuungcrtkjq39.1360205764360@email.android.com> Love the bond it does not break down very often for me and service is great i can run all the antibodies i want for me even research animal because i have a more open research unit cleaning the covertiles is a little tedious but we make it work and replace them with new ones often beats disposing of all the waste from liquid cs generated by ventana in my experience From pruegg <@t> ihctech.net Thu Feb 7 13:02:39 2013 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Thu Feb 7 23:31:04 2013 Subject: [Histonet] P16 on Leica Bond Message-ID: Really 1:4? From tgenade <@t> gmail.com Fri Feb 8 01:05:56 2013 From: tgenade <@t> gmail.com (Tyrone Genade) Date: Fri Feb 8 01:06:00 2013 Subject: [Histonet] PFA, postfixing and GalC & MBP antibodies Message-ID: Hello, I have previously cut frozen sections and post-fixed in methanol for 2 minutes and got OK staining using mono- and polyclonal antibodies to the lipid galactocerebroside (Galc). This has also been the case with MBP (myelin basic protein). I am now using PFA fixed tissue and then washing in acetone. This has given me much better tissue structure but now GalC (and I suppose MBP but I haven't tested it yet) immunoreactivity has been lost. I have used the antigen retrieval method of Inoue & Wittbrodt (http://www.ncbi.nlm.nih.gov/pubmed/21603650) but this antigen has not been rescued. I have washed in acetone for 2 and 5 minutes. Do you think this is an antigen retrieval/obliteration issue or the galactocerebroside simply being washed out by the acetone? If anyone has had any success with GalC antibodies and PFA fixation I would like to know about it! So far my searches haven't turned up anything useful. Thanks -- Dr Tyrone Genade Department of Human Biology University of Cape Town South Africa From kim.tournear <@t> yahoo.com Fri Feb 8 01:47:55 2013 From: kim.tournear <@t> yahoo.com (Kim Tournear) Date: Fri Feb 8 01:48:48 2013 Subject: [Histonet] Leica Bond III Opinions In-Reply-To: <02w02n0ntoljuungcrtkjq39.1360205764360@email.android.com> References: <02w02n0ntoljuungcrtkjq39.1360205764360@email.android.com> Message-ID: <07DAA8DE-42CF-43E6-AC7F-7289CD6CABAB@yahoo.com> The bond is great. A lot less waste to deal with and the maintenance is soooo easy. Less than 10 minutes. No tubing to unclog and disinfect. And the operating cost is much cheaper. And to top it off, it won't even give you the option to start a run until everything is right. No going back and forth to fix this, that, and whatever. It's truly a walk away system once you click start. Sent from the iPhone of Kim Tournear ?? ? On Feb 6, 2013, at 8:56 PM, Patsy Ruegg wrote: > Love the bond it does not break down very often for me and service is great i can run all the antibodies i want for me even research animal because i have a more open research unit cleaning the covertiles is a little tedious but we make it work and replace them with new ones often beats disposing of all the waste from liquid cs generated by ventana in my experience > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Susan.Walzer <@t> HCAHealthcare.com Fri Feb 8 02:03:09 2013 From: Susan.Walzer <@t> HCAHealthcare.com (Susan.Walzer@HCAHealthcare.com) Date: Fri Feb 8 02:03:07 2013 Subject: [Histonet] RE: Processor dehydration cycles.. In-Reply-To: <535971279F56F9458E646CB1B2AA53C50696124248@52VEJX-MV15-01.area52.afnoapps.usaf.mil> References: <535971279F56F9458E646CB1B2AA53C50696124248@52VEJX-MV15-01.area52.afnoapps.usaf.mil> Message-ID: <4BF03F5404EBDE409AF9232DA74B9DED2DFD29D6AD@FWDCWPMSGCMS09.hca.corpad.net> 70,80 95,95,100,100 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of PRESZLER, JEREMIAH C MSgt USAF AETC 59 LSQ/SGVLH Sent: Thursday, February 07, 2013 11:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FW: Processor dehydration cycles.. I agree with Joyce on this: Formalin salt precipitate tends to become more common if you start above 70%. WE use a 70%, then 80% and two 95% in our process here. Very Respectfully, Jeremiah C. Preszler, MSgt, USAF HT (ASCP) Flight Chief, Anatomic Pathology 959 CSPS/ SGVLH WHASC JBSA-Lackland AFB, TX 78236 (210) 292-5519 DSN: 662-5519 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Janine.SimmsColon <@t> jmmc.com Fri Feb 8 06:45:01 2013 From: Janine.SimmsColon <@t> jmmc.com (SimmsColon, Janine) Date: Fri Feb 8 06:45:05 2013 Subject: [Histonet] Cassette labeling Message-ID: Good morning all, I hope all here in the East are prepared for the storm, (considering I am in New England and it is February it is not all that surprising to me yet I am always amazed at the commotion that occurs with every impending snow, but I digress). I know many out there may have cassette labelers so this may not be an issue but for those who still label by hand I am curious as to how you label. At my previous lab besides the case number (S13-accession number), we also indicated how to embed the pieces (= for on edge), and the number of pieces in the cassette. I am just curious as to how common this practice is or if there is any other way you annotate the number of pieces expected in a cassette. Thank you in advance for any feedback you provide as I have received many helpful responses to my previous posts. Janine Simms Colon, BHA, CPhT, HT(ASCP) Histology/Pathology Johnson Memorial Hospital 201 Chestnut Hill Road Stafford Springs, CT 06076 Office: 860-684-8230 ext. 5197 janine.simmscolon@jmmc.com The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. From BSullivan <@t> virtua.org Fri Feb 8 06:48:46 2013 From: BSullivan <@t> virtua.org (Sullivan, Beatrice) Date: Fri Feb 8 06:48:51 2013 Subject: [Histonet] RE: Cassette labeling In-Reply-To: References: Message-ID: <6932520047F7EE46B512E9801344F160046738@ExchangeMB-1.Virtua.org> We do likewise. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of SimmsColon, Janine Sent: Friday, February 08, 2013 7:45 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cassette labeling Good morning all, I hope all here in the East are prepared for the storm, (considering I am in New England and it is February it is not all that surprising to me yet I am always amazed at the commotion that occurs with every impending snow, but I digress). I know many out there may have cassette labelers so this may not be an issue but for those who still label by hand I am curious as to how you label. At my previous lab besides the case number (S13-accession number), we also indicated how to embed the pieces (= for on edge), and the number of pieces in the cassette. I am just curious as to how common this practice is or if there is any other way you annotate the number of pieces expected in a cassette. Thank you in advance for any feedback you provide as I have received many helpful responses to my previous posts. Janine Simms Colon, BHA, CPhT, HT(ASCP) Histology/Pathology Johnson Memorial Hospital 201 Chestnut Hill Road Stafford Springs, CT 06076 Office: 860-684-8230 ext. 5197 janine.simmscolon@jmmc.com The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message, and any included attachments, are from Virtua Health or its related affiliates and is intended only for the addressee(s). The information contained herein is privileged, proprietary or may include confidential information and/or protected patient health information. Any unauthorized review, forwarding, printing, copying, distributing, or otherwise disseminating or taking any action based on such information is strictly prohibited. If you have received this message in error, or have reason to believe you are not authorized to receive it, please delete this message promptly and notify the sender by e-mail with a copy to ISSECURITY@virtua.org. Thank you From BSullivan <@t> virtua.org Fri Feb 8 06:52:29 2013 From: BSullivan <@t> virtua.org (Sullivan, Beatrice) Date: Fri Feb 8 06:52:34 2013 Subject: [Histonet] Temperatures Message-ID: <6932520047F7EE46B512E9801344F16004674B@ExchangeMB-1.Virtua.org> I'm looking for a fix to our problem of no temperatures being taken on the weekends. We are closed and this is creating an issue. Our processors are not running until Sunday night but the paraffin in both the processors and embedding center are kept molten. Any help would be greatly appreciated Beatrice L. Sullivan HT(ASCP)HTL Corporate Histology Manager Virtua, Voorhees 856-247-3144 This message, and any included attachments, are from Virtua Health or its related affiliates and is intended only for the addressee(s). The information contained herein is privileged, proprietary or may include confidential information and/or protected patient health information. Any unauthorized review, forwarding, printing, copying, distributing, or otherwise disseminating or taking any action based on such information is strictly prohibited. If you have received this message in error, or have reason to believe you are not authorized to receive it, please delete this message promptly and notify the sender by e-mail with a copy to ISSECURITY@virtua.org. Thank you From MMargiotta <@t> bmhmc.org Fri Feb 8 06:54:49 2013 From: MMargiotta <@t> bmhmc.org (Margiotta-Watz, Michele) Date: Fri Feb 8 06:54:54 2013 Subject: [Histonet] RE: Cassette labeling In-Reply-To: References: Message-ID: <230D0B9EC57D7A45A7A186C6AB4C7ABC296E340D@BMH-EXCHANGE-01.BMHMC.ORG> We print out an embedding log and note the # of pieces/block from the Doc's dictation. We are small scale so it works fine for us. Michele -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of SimmsColon, Janine Sent: Friday, February 08, 2013 7:45 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cassette labeling Good morning all, I hope all here in the East are prepared for the storm, (considering I am in New England and it is February it is not all that surprising to me yet I am always amazed at the commotion that occurs with every impending snow, but I digress). I know many out there may have cassette labelers so this may not be an issue but for those who still label by hand I am curious as to how you label. At my previous lab besides the case number (S13-accession number), we also indicated how to embed the pieces (= for on edge), and the number of pieces in the cassette. I am just curious as to how common this practice is or if there is any other way you annotate the number of pieces expected in a cassette. Thank you in advance for any feedback you provide as I have received many helpful responses to my previous posts. Janine Simms Colon, BHA, CPhT, HT(ASCP) Histology/Pathology Johnson Memorial Hospital 201 Chestnut Hill Road Stafford Springs, CT 06076 Office: 860-684-8230 ext. 5197 janine.simmscolon@jmmc.com The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet DISCLAIMER: This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. From cls71877 <@t> gmail.com Fri Feb 8 07:32:36 2013 From: cls71877 <@t> gmail.com (Cristi Rigazio) Date: Fri Feb 8 07:32:43 2013 Subject: [Histonet] Cassette labeling In-Reply-To: References: Message-ID: <97951FEB-8C5B-4DED-87D3-BC655DFE3E18@gmail.com> We print out a procedure list that has the number of pieces on it and it next to the embedding center for reference while performing that task. The only we write on the cassette is the accession number. Thanks, Cristi Sent from my iPhone On Feb 8, 2013, at 4:45 AM, "SimmsColon, Janine" wrote: > Good morning all, > > > > I hope all here in the East are prepared for the storm, (considering I > am in New England and it is February it is not all that surprising to me > yet I am always amazed at the commotion that occurs with every impending > snow, but I digress). I know many out there may have cassette labelers > so this may not be an issue but for those who still label by hand I am > curious as to how you label. At my previous lab besides the case number > (S13-accession number), we also indicated how to embed the pieces (= for > on edge), and the number of pieces in the cassette. I am just curious as > to how common this practice is or if there is any other way you annotate > the number of pieces expected in a cassette. Thank you in advance for > any feedback you provide as I have received many helpful responses to my > previous posts. > > > > Janine Simms Colon, BHA, CPhT, HT(ASCP) > > Histology/Pathology > > Johnson Memorial Hospital > > 201 Chestnut Hill Road > > Stafford Springs, CT 06076 > > Office: 860-684-8230 ext. 5197 > > janine.simmscolon@jmmc.com > > > > > The information contained in this message may be privileged and confidential and protected from disclosure. > If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering > this message to the intended recipient, you are hereby notified that any dissemination, distribution or > copying of this communication is strictly prohibited. If you have received this communication in error, please > notify us immediately by replying to the message and deleting it from your computer. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Nancy_Schmitt <@t> pa-ucl.com Fri Feb 8 07:57:13 2013 From: Nancy_Schmitt <@t> pa-ucl.com (Nancy Schmitt) Date: Fri Feb 8 07:57:17 2013 Subject: [Histonet] hand pap stains for non-gyn specimens/smears Message-ID: <906B4DA90ED1DB4DB6C7E94D7CEE6C364DC6D1AA@PEITHA.wad.pa-ucl.com> Good Morning- Would anyone be willing to share their process for pap stains - to include times in all steps and brand of stain you are using. We are trying to clean up some issues we are having. Thanks so much- Nancy Schmitt HT, MLT (ASCP) Histology Coordinator Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From Susan.Walzer <@t> HCAHealthcare.com Fri Feb 8 08:30:54 2013 From: Susan.Walzer <@t> HCAHealthcare.com (Susan.Walzer@HCAHealthcare.com) Date: Fri Feb 8 08:31:00 2013 Subject: [Histonet] RE: Temperatures In-Reply-To: <6932520047F7EE46B512E9801344F16004674B@ExchangeMB-1.Virtua.org> References: <6932520047F7EE46B512E9801344F16004674B@ExchangeMB-1.Virtua.org> Message-ID: <4BF03F5404EBDE409AF9232DA74B9DED2DFD29D949@FWDCWPMSGCMS09.hca.corpad.net> We have the clinical lab check our friges and freezers on the weekends..everything else is off. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sullivan, Beatrice Sent: Friday, February 08, 2013 7:52 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Temperatures I'm looking for a fix to our problem of no temperatures being taken on the weekends. We are closed and this is creating an issue. Our processors are not running until Sunday night but the paraffin in both the processors and embedding center are kept molten. Any help would be greatly appreciated Beatrice L. Sullivan HT(ASCP)HTL Corporate Histology Manager Virtua, Voorhees 856-247-3144 This message, and any included attachments, are from Virtua Health or its related affiliates and is intended only for the addressee(s). The information contained herein is privileged, proprietary or may include confidential information and/or protected patient health information. Any unauthorized review, forwarding, printing, copying, distributing, or otherwise disseminating or taking any action based on such information is strictly prohibited. If you have received this message in error, or have reason to believe you are not authorized to receive it, please delete this message promptly and notify the sender by e-mail with a copy to ISSECURITY@virtua.org. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Feb 8 09:16:59 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Feb 8 09:17:06 2013 Subject: [Histonet] Temperatures In-Reply-To: <6932520047F7EE46B512E9801344F16004674B@ExchangeMB-1.Virtua.org> References: <6932520047F7EE46B512E9801344F16004674B@ExchangeMB-1.Virtua.org> Message-ID: <1360336619.9602.YahooMailNeo@web163105.mail.bf1.yahoo.com> And my question is: why are you bothered by not knowing the temp. in a processor that only starts to process on Sundays? If the processor is emptied on Mondays to embed?cut, take the temp. then. Other that this you would have to place a sensor and a recording device or you would have to make somebody to go to the lab. For just write down the melted paraffin temp.? Too much expense and trouble for an non-existent problem. Ren? J. From: "Sullivan, Beatrice" To: "histonet@lists.utsouthwestern.edu" Sent: Friday, February 8, 2013 7:52 AM Subject: [Histonet] Temperatures I'm looking for a fix to our problem of no temperatures being taken on the weekends. We are closed and this is creating an issue. Our processors are not running until Sunday night but the paraffin in both the processors and embedding center are kept molten. Any help would be greatly appreciated Beatrice L. Sullivan HT(ASCP)HTL Corporate Histology Manager Virtua, Voorhees 856-247-3144 This message, and any included attachments, are from Virtua Health or its related affiliates and is intended only for the addressee(s). The information contained herein is privileged, proprietary or may include confidential information and/or protected patient health information. Any unauthorized review, forwarding, printing, copying, distributing, or otherwise disseminating or taking any action based on such information is strictly prohibited. If you have received this message in error, or have reason to believe you are not authorized to receive it, please delete this message promptly and notify the sender by e-mail with a copy to ISSECURITY@virtua.org. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BSullivan <@t> virtua.org Fri Feb 8 09:32:22 2013 From: BSullivan <@t> virtua.org (Sullivan, Beatrice) Date: Fri Feb 8 09:32:27 2013 Subject: [Histonet] Temperatures In-Reply-To: <1360336619.9602.YahooMailNeo@web163105.mail.bf1.yahoo.com> References: <6932520047F7EE46B512E9801344F16004674B@ExchangeMB-1.Virtua.org> <1360336619.9602.YahooMailNeo@web163105.mail.bf1.yahoo.com> Message-ID: <6932520047F7EE46B512E9801344F1600467CC@ExchangeMB-1.Virtua.org> Because I have been told that according to JCAHO (who will be inspecting us) that if a temperature is required to ascertain consistency, quality and integrity, you will have to record temperature 7 days per week. From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: Friday, February 08, 2013 10:17 AM To: Sullivan, Beatrice; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Temperatures And my question is: why are you bothered by not knowing the temp. in a processor that only starts to process on Sundays? If the processor is emptied on Mondays to embed?cut, take the temp. then. Other that this you would have to place a sensor and a recording device or you would have to make somebody to go to the lab. For just write down the melted paraffin temp.? Too much expense and trouble for an non-existent problem. Ren? J. From: "Sullivan, Beatrice" > To: "histonet@lists.utsouthwestern.edu" > Sent: Friday, February 8, 2013 7:52 AM Subject: [Histonet] Temperatures I'm looking for a fix to our problem of no temperatures being taken on the weekends. We are closed and this is creating an issue. Our processors are not running until Sunday night but the paraffin in both the processors and embedding center are kept molten. Any help would be greatly appreciated Beatrice L. Sullivan HT(ASCP)HTL Corporate Histology Manager Virtua, Voorhees 856-247-3144 This message, and any included attachments, are from Virtua Health or its related affiliates and is intended only for the addressee(s). The information contained herein is privileged, proprietary or may include confidential information and/or protected patient health information. Any unauthorized review, forwarding, printing, copying, distributing, or otherwise disseminating or taking any action based on such information is strictly prohibited. If you have received this message in error, or have reason to believe you are not authorized to receive it, please delete this message promptly and notify the sender by e-mail with a copy to ISSECURITY@virtua.org. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message, and any included attachments, are from Virtua Health or its related affiliates and is intended only for the addressee(s). The information contained herein is privileged, proprietary or may include confidential information and/or protected patient health information. Any unauthorized review, forwarding, printing, copying, distributing, or otherwise disseminating or taking any action based on such information is strictly prohibited. If you have received this message in error, or have reason to believe you are not authorized to receive it, please delete this message promptly and notify the sender by e-mail with a copy to ISSECURITY@virtua.org. Thank you From TMcNemar <@t> lmhealth.org Fri Feb 8 10:22:59 2013 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Fri Feb 8 10:23:11 2013 Subject: [Histonet] Temperatures In-Reply-To: <6932520047F7EE46B512E9801344F1600467CC@ExchangeMB-1.Virtua.org> References: <6932520047F7EE46B512E9801344F16004674B@ExchangeMB-1.Virtua.org> <1360336619.9602.YahooMailNeo@web163105.mail.bf1.yahoo.com> <6932520047F7EE46B512E9801344F1600467CC@ExchangeMB-1.Virtua.org> Message-ID: We are JCAHO and this has never come up but that doesn't mean that it won't. I don?t know about your specific processor but ours (VIP5), will fail to start and alarm if the paraffin temp is out of range. Our processor is tied in to the main lab temperature monitoring system so we get a call anytime there is an alarm. Such a system is probably not feasible for a processor alone but does your main lab have anything in place? Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sullivan, Beatrice Sent: Friday, February 08, 2013 10:32 AM To: Rene J Buesa; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Temperatures Because I have been told that according to JCAHO (who will be inspecting us) that if a temperature is required to ascertain consistency, quality and integrity, you will have to record temperature 7 days per week. From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: Friday, February 08, 2013 10:17 AM To: Sullivan, Beatrice; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Temperatures And my question is: why are you bothered by not knowing the temp. in a processor that only starts to process on Sundays? If the processor is emptied on Mondays to embed?cut, take the temp. then. Other that this you would have to place a sensor and a recording device or you would have to make somebody to go to the lab. For just write down the melted paraffin temp.? Too much expense and trouble for an non-existent problem. Ren? J. From: "Sullivan, Beatrice" > To: "histonet@lists.utsouthwestern.edu" > Sent: Friday, February 8, 2013 7:52 AM Subject: [Histonet] Temperatures I'm looking for a fix to our problem of no temperatures being taken on the weekends. We are closed and this is creating an issue. Our processors are not running until Sunday night but the paraffin in both the processors and embedding center are kept molten. Any help would be greatly appreciated Beatrice L. Sullivan HT(ASCP)HTL Corporate Histology Manager Virtua, Voorhees 856-247-3144 This message, and any included attachments, are from Virtua Health or its related affiliates and is intended only for the addressee(s). The information contained herein is privileged, proprietary or may include confidential information and/or protected patient health information. Any unauthorized review, forwarding, printing, copying, distributing, or otherwise disseminating or taking any action based on such information is strictly prohibited. If you have received this message in error, or have reason to believe you are not authorized to receive it, please delete this message promptly and notify the sender by e-mail with a copy to ISSECURITY@virtua.org. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message, and any included attachments, are from Virtua Health or its related affiliates and is intended only for the addressee(s). The information contained herein is privileged, proprietary or may include confidential information and/or protected patient health information. Any unauthorized review, forwarding, printing, copying, distributing, or otherwise disseminating or taking any action based on such information is strictly prohibited. If you have received this message in error, or have reason to believe you are not authorized to receive it, please delete this message promptly and notify the sender by e-mail with a copy to ISSECURITY@virtua.org. Thank you This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From Melissa.Kuhnla <@t> chsli.org Fri Feb 8 10:23:04 2013 From: Melissa.Kuhnla <@t> chsli.org (Kuhnla, Melissa) Date: Fri Feb 8 10:23:15 2013 Subject: [Histonet] Temperatures In-Reply-To: <6932520047F7EE46B512E9801344F1600467CC@ExchangeMB-1.Virtua.org> References: <6932520047F7EE46B512E9801344F16004674B@ExchangeMB-1.Virtua.org><1360336619.9602.YahooMailNeo@web163105.mail.bf1.yahoo.com> <6932520047F7EE46B512E9801344F1600467CC@ExchangeMB-1.Virtua.org> Message-ID: Beatrice, How about...your processor monitors the temp. If it falls out of a certain= range it will most likely throw an error. This is not a recorded temp day= 6 and 7 but is in a sense a monitoring system. Our processors are set up = with an alarm system that call employees if certain errors occur. Do you h= ave something similar? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@li= sts.utsouthwestern.edu] On Behalf Of Sullivan, Beatrice Sent: Friday, February 08, 2013 10:32 AM To: Rene J Buesa; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Temperatures Because I have been told that according to JCAHO (who will be inspecting us= ) that if a temperature is required to ascertain consistency, quality and i= ntegrity, you will have to record temperature 7 days per week. From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: Friday, February 08, 2013 10:17 AM To: Sullivan, Beatrice; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Temperatures And my question is: why are you bothered by not knowing the temp. in a proc= essor that only starts to process on Sundays? If the processor is emptied on Mondays to embed=A1=FAcut, take the temp. th= en. Other that this you would have to place a sensor and a recording device or = you would have to make somebody to go to the lab. For just write down the m= elted paraffin temp.? Too much expense and trouble for an non-existent prob= lem. Ren=A8=A6 J. From: "Sullivan, Beatrice" > To: "histonet@lists.utsouthwestern.edu" > Sent: Friday, February 8, 2013 7:52 AM Subject: [Histonet] Temperatures I'm looking for a fix to our problem of no temperatures being taken on the = weekends. We are closed and this is creating an issue. Our processors are n= ot running until Sunday night but the paraffin in both the processors and e= mbedding center are kept molten. Any help would be greatly appreciated Beatrice L. Sullivan HT(ASCP)HTL Corporate Histology Manager Virtua, Voorhees 856-247-3144 This message, and any included attachments, are from Virtua Health or its r= elated affiliates and is intended only for the addressee(s). The informatio= n contained herein is privileged, proprietary or may include confidential i= nformation and/or protected patient health information. Any unauthorized re= view, forwarding, printing, copying, distributing, or otherwise disseminati= ng or taking any action based on such information is strictly prohibited. I= f you have received this message in error, or have reason to believe you ar= e not authorized to receive it, please delete this message promptly and not= ify the sender by e-mail with a copy to ISSECURITY@virtua.org. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu= http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message, and any included attachments, are from Virtua Health or its r= elated affiliates and is intended only for the addressee(s). The informatio= n contained herein is privileged, proprietary or may include confidential i= nformation and/or protected patient health information. Any unauthorized re= view, forwarding, printing, copying, distributing, or otherwise disseminati= ng or taking any action based on such information is strictly prohibited. I= f you have received this message in error, or have reason to believe you ar= e not authorized to receive it, please delete this message promptly and not= ify the sender by e-mail with a copy to ISSECURITY@virtua.org. = Thank you The information in this e-mail, and any attachments therein, is confidentia= l and for use by the intended addressee only. If this message is received b= y you in error please do not disseminate or read further. Please reply to t= he sender that you have received the message in error, then delete the mess= age. Although Catholic Health Services of Long Island attempts to sweep e-m= ail and attachments for viruses, it does not guarantee that either are vir= us-free and accepts no liability for any damage sustained as a result of vi= ruses. Thank you. From tpodawiltz <@t> lrgh.org Fri Feb 8 10:49:04 2013 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Fri Feb 8 10:49:10 2013 Subject: [Histonet] RE: Cassette labeling In-Reply-To: <230D0B9EC57D7A45A7A186C6AB4C7ABC296E340D@BMH-EXCHANGE-01.BMHMC.ORG> References: <230D0B9EC57D7A45A7A186C6AB4C7ABC296E340D@BMH-EXCHANGE-01.BMHMC.ORG> Message-ID: <38667E7FB77ECD4E91BFAEB8D986386324FCE3BE73@LRGHEXVS1.practice.lrgh.org> That is basically what we do. Tom -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Margiotta-Watz, Michele Sent: Friday, February 08, 2013 7:55 AM To: 'SimmsColon, Janine'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Cassette labeling We print out an embedding log and note the # of pieces/block from the Doc's dictation. We are small scale so it works fine for us. Michele -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of SimmsColon, Janine Sent: Friday, February 08, 2013 7:45 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cassette labeling Good morning all, I hope all here in the East are prepared for the storm, (considering I am in New England and it is February it is not all that surprising to me yet I am always amazed at the commotion that occurs with every impending snow, but I digress). I know many out there may have cassette labelers so this may not be an issue but for those who still label by hand I am curious as to how you label. At my previous lab besides the case number (S13-accession number), we also indicated how to embed the pieces (= for on edge), and the number of pieces in the cassette. I am just curious as to how common this practice is or if there is any other way you annotate the number of pieces expected in a cassette. Thank you in advance for any feedback you provide as I have received many helpful responses to my previous posts. Janine Simms Colon, BHA, CPhT, HT(ASCP) Histology/Pathology Johnson Memorial Hospital 201 Chestnut Hill Road Stafford Springs, CT 06076 Office: 860-684-8230 ext. 5197 janine.simmscolon@jmmc.com The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet DISCLAIMER: This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From patpxs <@t> gmail.com Fri Feb 8 11:41:36 2013 From: patpxs <@t> gmail.com (Paula Sicurello) Date: Fri Feb 8 11:41:39 2013 Subject: [Histonet] RE: Temperatures In-Reply-To: <4BF03F5404EBDE409AF9232DA74B9DED2DFD29D949@FWDCWPMSGCMS09.hca.corpad.net> References: <6932520047F7EE46B512E9801344F16004674B@ExchangeMB-1.Virtua.org> <4BF03F5404EBDE409AF9232DA74B9DED2DFD29D949@FWDCWPMSGCMS09.hca.corpad.net> Message-ID: We have NIST traceable digital thermometers that can have the min/max reset. We have been told that if you reset on Friday and check on Monday morning the min/max & it is in range-put an OK by the date. -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 On Fri, Feb 8, 2013 at 9:30 AM, wrote: > We have the clinical lab check our friges and freezers on the weekends..everything else is off. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sullivan, Beatrice > Sent: Friday, February 08, 2013 7:52 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Temperatures > > I'm looking for a fix to our problem of no temperatures being taken on the weekends. We are closed and this is creating an issue. Our processors are not running until Sunday night but the paraffin in both the processors and embedding center are kept molten. Any help would be greatly appreciated > > Beatrice L. Sullivan HT(ASCP)HTL > Corporate Histology Manager > Virtua, Voorhees > 856-247-3144 > > > This message, and any included attachments, are from Virtua Health or its related affiliates and is intended only for the addressee(s). The information contained herein is privileged, proprietary or may include confidential information and/or protected patient health information. Any unauthorized review, forwarding, printing, copying, distributing, or otherwise disseminating or taking any action based on such information is strictly prohibited. If you have received this message in error, or have reason to believe you are not authorized to receive it, please delete this message promptly and notify the sender by e-mail with a copy to ISSECURITY@virtua.org. > > Thank you > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cathy.crumpton <@t> tuality.org Fri Feb 8 11:58:28 2013 From: cathy.crumpton <@t> tuality.org (Cathy Crumpton) Date: Fri Feb 8 11:58:35 2013 Subject: [Histonet] PIN4 staining Message-ID: We use Bond slides and have noticed that occasionally they seem to have an extra thick coating on them that will pick up DAB and turn slides brown. If the glass itself is turning brown this might be your issue. Have you tried different slide lots or brands? Cathy Crumpton HT(ASCP), Lead Histotechnician Tuality Community Hospital From KJohnson <@t> med.miami.edu Fri Feb 8 12:00:00 2013 From: KJohnson <@t> med.miami.edu (Johnson, Kevin) Date: Fri Feb 8 12:00:07 2013 Subject: [Histonet] Could these samples have been saved? Message-ID: <59F7809368E0084C8AE6B38C93D735EB2FA103F52A@MEDEXMB02.ad.med.miami.edu> Dear all, During an overnight tissue processing cycle, a malfunction occurred such that the sample basket was suspended in mid-air for several hours at probably the worst spot in which to do so---after the final absolute ethanol of the dehydration series. I continued the process manually in the morning, and carried it through blocking and attempted sectioning. However, the samples (mouse skin and fat) had been converted to uncuttable rocks. In hindsight, should I have attempted to rehydrate and reprocess these samples in an attempt to glean even minimal information from them? Or is there no way to unmummify a mummy? Regards, Kevin Johnson University of Miami Diabetes Research Institute From BGapinski <@t> pathgroup.com Fri Feb 8 12:00:11 2013 From: BGapinski <@t> pathgroup.com (Bruce Gapinski) Date: Fri Feb 8 12:03:11 2013 Subject: [Histonet] What's on that H&E? Message-ID: Dear Histonians, How many sections do put on a slide from a block cast in a small embedding mold? Do you automatically include any levels? Then please tell me how many slides your techs cut per hour? The problem is (may be) that we put 4 levels on a slide with two sections per level. That is: 1. Full face (or pretty close) 2 sections 2. Level 2 sections 3. Level 2 sections 4. Level 2 sections So I have 8 sections on my H&E. This takes me some time, and I understand all histologists may not be doing things this way. But the problem is, when we discuss output and my techs appear slow I find that other labs put much less tissue on a slide. Some labs give a fatty string of serial sections in two rows, and that appears to be just like my slide. But only on the surface (pardon the pun). My pathologists just renegotiated the contracts for all our dermatologists after the new 88305TC pricing, and those doctors say "Oh, we can get slides cheaper than that." Maybe they can but what would they see on the H&E, and would they care? So now I'm faced with doing things as we have or stop giving those levels. I understand my job as a Histologist is to demonstrate the tissue. So I'll stick to the levels, because I: 1. Work for the patient 2. Am supervised by the Pathologists 3. Am reimbursed by our company Tell me what you do in you lab, please Bruce Gapinsk HT (ASCP) Chief Histologist Marin Medical Laboratories PathGroup SF ________________________________ Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you From kgrobert <@t> rci.rutgers.edu Fri Feb 8 12:22:15 2013 From: kgrobert <@t> rci.rutgers.edu (kgrobert@rci.rutgers.edu) Date: Fri Feb 8 12:22:47 2013 Subject: [Histonet] Could these samples have been saved? In-Reply-To: <59F7809368E0084C8AE6B38C93D735EB2FA103F52A@MEDEXMB02.ad.med.miami.edu > References: <59F7809368E0084C8AE6B38C93D735EB2FA103F52A@MEDEXMB02.ad.med.miami.edu> Message-ID: <9302f92021acbe8e570f36e8cfb283ad.squirrel@webmail.rci.rutgers.edu> I don't know about your samples now, but for future reference, you might find this interesting: http://realhistoryww.com/world_history/ancient/Misc/Data/Study_mummified_soft_tissues.htm Kathleen Roberts Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology & Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (848) 445-1443 FAX (732) 445-6905 > Dear all, > > During an overnight tissue processing cycle, a malfunction occurred such > that the sample basket was suspended in mid-air for several hours at > probably the worst spot in which to do so---after the final absolute > ethanol of the dehydration series. I continued the process manually in the > morning, and carried it through blocking and attempted sectioning. > However, the samples (mouse skin and fat) had been converted to uncuttable > rocks. > > In hindsight, should I have attempted to rehydrate and reprocess these > samples in an attempt to glean even minimal information from them? Or is > there no way to unmummify a mummy? > > Regards, > > Kevin Johnson > University of Miami > Diabetes Research Institute > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From rheyna <@t> lumc.edu Fri Feb 8 12:22:10 2013 From: rheyna <@t> lumc.edu (Roger Heyna) Date: Fri Feb 8 12:22:49 2013 Subject: [Histonet] Could these samples have been saved? In-Reply-To: <59F7809368E0084C8AE6B38C93D735EB2FA103F52A@MEDEXMB02.ad.med.miami.edu> References: <59F7809368E0084C8AE6B38C93D735EB2FA103F52A@MEDEXMB02.ad.med.miami.edu> Message-ID: <5114EDF20200002300049F0E@gwgwia1.luhs.org> We microwave process our tissues, and we've had tissues fall out of the rack after the dehydration phases and air dry in the open retort for hours. We did not continue with processing; we added them to formalin for eight hours (which could be overkill) and then started the processing from the beginning. We had no problems with cutting or staining. If you look in Freida Carson's Histotechnology: A Self-Instructional Text, she includes a protocol for tissues that were well-fixed but accidentally desiccated. She suggests the following: 1. If the tissue has been in paraffin, blot off as much as possible with paper towels. 2. Soak the tissues overnight in a rehydrating solution: 50 mL water, 30 mL absolute alcohol, and 20 mL of 5% aqueous solution of sodium carbonate. 3. Reprocess as usual. I've never done this before, and maybe others have modified it for labs without sodium carbonate. Roger Heyna Maywood, IL >>> "Johnson, Kevin" 2/8/2013 12:00 PM >>> Dear all, During an overnight tissue processing cycle, a malfunction occurred such that the sample basket was suspended in mid-air for several hours at probably the worst spot in which to do so---after the final absolute ethanol of the dehydration series. I continued the process manually in the morning, and carried it through blocking and attempted sectioning. However, the samples (mouse skin and fat) had been converted to uncuttable rocks. In hindsight, should I have attempted to rehydrate and reprocess these samples in an attempt to glean even minimal information from them? Or is there no way to unmummify a mummy? Regards, Kevin Johnson University of Miami Diabetes Research Institute _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Albert.Santiago <@t> uphs.upenn.edu Fri Feb 8 12:59:40 2013 From: Albert.Santiago <@t> uphs.upenn.edu (Santiago, Albert) Date: Fri Feb 8 12:59:53 2013 Subject: [Histonet] RE: Histonet Digest, Vol 111, Issue 13 In-Reply-To: References: Message-ID: <63C408E65F57294790C8CFA2150A6475B17976@uphmasphi013.UPHS.PENNHEALTH.PRV> Hi Janine, we use cassette printers but we still write the number of pieces on one side of the cassette and any special instructions on the other side, such as embed on edge or embed on red ink, etc. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Friday, February 08, 2013 1:01 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 111, Issue 13 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: Temperatures (Sullivan, Beatrice) 2. RE: Temperatures (Tom McNemar) 3. RE: Temperatures (Kuhnla, Melissa) 4. RE: Cassette labeling (Podawiltz, Thomas) 5. Re: RE: Temperatures (Paula Sicurello) 6. PIN4 staining (Cathy Crumpton) ---------------------------------------------------------------------- Message: 1 Date: Fri, 8 Feb 2013 15:32:22 +0000 From: "Sullivan, Beatrice" Subject: RE: [Histonet] Temperatures To: Rene J Buesa , "histonet@lists.utsouthwestern.edu" Message-ID: <6932520047F7EE46B512E9801344F1600467CC@ExchangeMB-1.Virtua.org> Content-Type: text/plain; charset="utf-8" Because I have been told that according to JCAHO (who will be inspecting us) that if a temperature is required to ascertain consistency, quality and integrity, you will have to record temperature 7 days per week. From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: Friday, February 08, 2013 10:17 AM To: Sullivan, Beatrice; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Temperatures And my question is: why are you bothered by not knowing the temp. in a processor that only starts to process on Sundays? If the processor is emptied on Mondays to embed???cut, take the temp. then. Other that this you would have to place a sensor and a recording device or you would have to make somebody to go to the lab. For just write down the melted paraffin temp.? Too much expense and trouble for an non-existent problem. Ren?? J. From: "Sullivan, Beatrice" > To: "histonet@lists.utsouthwestern.edu" > Sent: Friday, February 8, 2013 7:52 AM Subject: [Histonet] Temperatures I'm looking for a fix to our problem of no temperatures being taken on the weekends. We are closed and this is creating an issue. Our processors are not running until Sunday night but the paraffin in both the processors and embedding center are kept molten. Any help would be greatly appreciated Beatrice L. Sullivan HT(ASCP)HTL Corporate Histology Manager Virtua, Voorhees 856-247-3144 This message, and any included attachments, are from Virtua Health or its related affiliates and is intended only for the addressee(s). The information contained herein is privileged, proprietary or may include confidential information and/or protected patient health information. Any unauthorized review, forwarding, printing, copying, distributing, or otherwise disseminating or taking any action based on such information is strictly prohibited. If you have received this message in error, or have reason to believe you are not authorized to receive it, please delete this message promptly and notify the sender by e-mail with a copy to ISSECURITY@virtua.org. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message, and any included attachments, are from Virtua Health or its related affiliates and is intended only for the addressee(s). The information contained herein is privileged, proprietary or may include confidential information and/or protected patient health information. Any unauthorized review, forwarding, printing, copying, distributing, or otherwise disseminating or taking any action based on such information is strictly prohibited. If you have received this message in error, or have reason to believe you are not authorized to receive it, please delete this message promptly and notify the sender by e-mail with a copy to ISSECURITY@virtua.org. Thank you ------------------------------ Message: 2 Date: Fri, 8 Feb 2013 11:22:59 -0500 From: Tom McNemar Subject: RE: [Histonet] Temperatures To: "'Sullivan, Beatrice'" , Rene J Buesa , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="utf-8" We are JCAHO and this has never come up but that doesn't mean that it won't. I don???t know about your specific processor but ours (VIP5), will fail to start and alarm if the paraffin temp is out of range. Our processor is tied in to the main lab temperature monitoring system so we get a call anytime there is an alarm. Such a system is probably not feasible for a processor alone but does your main lab have anything in place? Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sullivan, Beatrice Sent: Friday, February 08, 2013 10:32 AM To: Rene J Buesa; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Temperatures Because I have been told that according to JCAHO (who will be inspecting us) that if a temperature is required to ascertain consistency, quality and integrity, you will have to record temperature 7 days per week. From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: Friday, February 08, 2013 10:17 AM To: Sullivan, Beatrice; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Temperatures And my question is: why are you bothered by not knowing the temp. in a processor that only starts to process on Sundays? If the processor is emptied on Mondays to embed???cut, take the temp. then. Other that this you would have to place a sensor and a recording device or you would have to make somebody to go to the lab. For just write down the melted paraffin temp.? Too much expense and trouble for an non-existent problem. Ren?? J. From: "Sullivan, Beatrice" > To: "histonet@lists.utsouthwestern.edu" > Sent: Friday, February 8, 2013 7:52 AM Subject: [Histonet] Temperatures I'm looking for a fix to our problem of no temperatures being taken on the weekends. We are closed and this is creating an issue. Our processors are not running until Sunday night but the paraffin in both the processors and embedding center are kept molten. Any help would be greatly appreciated Beatrice L. Sullivan HT(ASCP)HTL Corporate Histology Manager Virtua, Voorhees 856-247-3144 This message, and any included attachments, are from Virtua Health or its related affiliates and is intended only for the addressee(s). The information contained herein is privileged, proprietary or may include confidential information and/or protected patient health information. Any unauthorized review, forwarding, printing, copying, distributing, or otherwise disseminating or taking any action based on such information is strictly prohibited. If you have received this message in error, or have reason to believe you are not authorized to receive it, please delete this message promptly and notify the sender by e-mail with a copy to ISSECURITY@virtua.org. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message, and any included attachments, are from Virtua Health or its related affiliates and is intended only for the addressee(s). The information contained herein is privileged, proprietary or may include confidential information and/or protected patient health information. Any unauthorized review, forwarding, printing, copying, distributing, or otherwise disseminating or taking any action based on such information is strictly prohibited. If you have received this message in error, or have reason to believe you are not authorized to receive it, please delete this message promptly and notify the sender by e-mail with a copy to ISSECURITY@virtua.org. Thank you This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ------------------------------ Message: 3 Date: Fri, 8 Feb 2013 11:23:04 -0500 From: "Kuhnla, Melissa" Subject: RE: [Histonet] Temperatures To: "Sullivan, Beatrice" , Rene J Buesa , Message-ID: Content-Type: text/plain; charset="gb2312" Beatrice, How about...your processor monitors the temp. If it falls out of a certain range it will most likely throw an error. This is not a recorded temp day 6 and 7 but is in a sense a monitoring system. Our processors are set up with an alarm system that call employees if certain errors occur. Do you have something similar? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sullivan, Beatrice Sent: Friday, February 08, 2013 10:32 AM To: Rene J Buesa; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Temperatures Because I have been told that according to JCAHO (who will be inspecting us) that if a temperature is required to ascertain consistency, quality and integrity, you will have to record temperature 7 days per week. From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: Friday, February 08, 2013 10:17 AM To: Sullivan, Beatrice; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Temperatures And my question is: why are you bothered by not knowing the temp. in a processor that only starts to process on Sundays? If the processor is emptied on Mondays to embed??cut, take the temp. then. Other that this you would have to place a sensor and a recording device or you would have to make somebody to go to the lab. For just write down the melted paraffin temp.? Too much expense and trouble for an non-existent problem. Ren?? J. From: "Sullivan, Beatrice" > To: "histonet@lists.utsouthwestern.edu" > Sent: Friday, February 8, 2013 7:52 AM Subject: [Histonet] Temperatures I'm looking for a fix to our problem of no temperatures being taken on the weekends. We are closed and this is creating an issue. Our processors are not running until Sunday night but the paraffin in both the processors and embedding center are kept molten. Any help would be greatly appreciated Beatrice L. Sullivan HT(ASCP)HTL Corporate Histology Manager Virtua, Voorhees 856-247-3144 This message, and any included attachments, are from Virtua Health or its related affiliates and is intended only for the addressee(s). The information contained herein is privileged, proprietary or may include confidential information and/or protected patient health information. Any unauthorized review, forwarding, printing, copying, distributing, or otherwise disseminating or taking any action based on such information is strictly prohibited. If you have received this message in error, or have reason to believe you are not authorized to receive it, please delete this message promptly and notify the sender by e-mail with a copy to ISSECURITY@virtua.org. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message, and any included attachments, are from Virtua Health or its related affiliates and is intended only for the addressee(s). The information contained herein is privileged, proprietary or may include confidential information and/or protected patient health information. Any unauthorized review, forwarding, printing, copying, distributing, or otherwise disseminating or taking any action based on such information is strictly prohibited. If you have received this message in error, or have reason to believe you are not authorized to receive it, please delete this message promptly and notify the sender by e-mail with a copy to ISSECURITY@virtua.org. Thank you The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. ------------------------------ Message: 4 Date: Fri, 8 Feb 2013 11:49:04 -0500 From: "Podawiltz, Thomas" Subject: [Histonet] RE: Cassette labeling To: "Margiotta-Watz, Michele" , "'SimmsColon, Janine'" , "histonet@lists.utsouthwestern.edu" Message-ID: <38667E7FB77ECD4E91BFAEB8D986386324FCE3BE73@LRGHEXVS1.practice.lrgh.org> Content-Type: text/plain; charset="us-ascii" That is basically what we do. Tom -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Margiotta-Watz, Michele Sent: Friday, February 08, 2013 7:55 AM To: 'SimmsColon, Janine'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Cassette labeling We print out an embedding log and note the # of pieces/block from the Doc's dictation. We are small scale so it works fine for us. Michele -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of SimmsColon, Janine Sent: Friday, February 08, 2013 7:45 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cassette labeling Good morning all, I hope all here in the East are prepared for the storm, (considering I am in New England and it is February it is not all that surprising to me yet I am always amazed at the commotion that occurs with every impending snow, but I digress). I know many out there may have cassette labelers so this may not be an issue but for those who still label by hand I am curious as to how you label. At my previous lab besides the case number (S13-accession number), we also indicated how to embed the pieces (= for on edge), and the number of pieces in the cassette. I am just curious as to how common this practice is or if there is any other way you annotate the number of pieces expected in a cassette. Thank you in advance for any feedback you provide as I have received many helpful responses to my previous posts. Janine Simms Colon, BHA, CPhT, HT(ASCP) Histology/Pathology Johnson Memorial Hospital 201 Chestnut Hill Road Stafford Springs, CT 06076 Office: 860-684-8230 ext. 5197 janine.simmscolon@jmmc.com The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. 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If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. ------------------------------ Message: 5 Date: Fri, 8 Feb 2013 12:41:36 -0500 From: Paula Sicurello Subject: Re: [Histonet] RE: Temperatures To: Susan.Walzer@hcahealthcare.com Cc: histonet@lists.utsouthwestern.edu, BSullivan@virtua.org Message-ID: Content-Type: text/plain; charset=ISO-8859-1 We have NIST traceable digital thermometers that can have the min/max reset. We have been told that if you reset on Friday and check on Monday morning the min/max & it is in range-put an OK by the date. -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 On Fri, Feb 8, 2013 at 9:30 AM, wrote: > We have the clinical lab check our friges and freezers on the weekends..everything else is off. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sullivan, Beatrice > Sent: Friday, February 08, 2013 7:52 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Temperatures > > I'm looking for a fix to our problem of no temperatures being taken on the weekends. We are closed and this is creating an issue. Our processors are not running until Sunday night but the paraffin in both the processors and embedding center are kept molten. Any help would be greatly appreciated > > Beatrice L. Sullivan HT(ASCP)HTL > Corporate Histology Manager > Virtua, Voorhees > 856-247-3144 > > > This message, and any included attachments, are from Virtua Health or its related affiliates and is intended only for the addressee(s). The information contained herein is privileged, proprietary or may include confidential information and/or protected patient health information. Any unauthorized review, forwarding, printing, copying, distributing, or otherwise disseminating or taking any action based on such information is strictly prohibited. If you have received this message in error, or have reason to believe you are not authorized to receive it, please delete this message promptly and notify the sender by e-mail with a copy to ISSECURITY@virtua.org. > > Thank you > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Fri, 8 Feb 2013 17:58:28 +0000 From: Cathy Crumpton Subject: [Histonet] PIN4 staining To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" We use Bond slides and have noticed that occasionally they seem to have an extra thick coating on them that will pick up DAB and turn slides brown. If the glass itself is turning brown this might be your issue. Have you tried different slide lots or brands? Cathy Crumpton HT(ASCP), Lead Histotechnician Tuality Community Hospital ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 111, Issue 13 ***************************************** The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From BZIMMERM <@t> gru.edu Fri Feb 8 13:13:15 2013 From: BZIMMERM <@t> gru.edu (Zimmerman, Billie) Date: Fri Feb 8 13:13:23 2013 Subject: [Histonet] Georgia Society for Histotechnology 40th year Celebration Message-ID: <7B3DEB32E69C034EACB479059C5DE3FF754F96@EX-MLB-03.ad.georgiahealth.edu> Please join us on beautiful Jekyll Island this April 12-14, 2013. If you register by March 1, 2013, you will receive the discounted "all inclusive" rate of $115. After March 1st, the "all inclusive" rate is $135. This rate includes the vendor reception on Friday night, celebration luncheon on Saturday, as well as a continental breakfast both Saturday and Sunday. In addition, you will receive all your CEU's through NSH with a possible total of 15 CEU's. Registration and room reservations are on our website at the "symposium" link. www.histosearch.com/gsh/ I encourage you to join or renew your FREE membership with the Georgia Society for Histotechnology. We have an impressive offering of accomplished speakers from our discipline. Please don't miss this affordable opportunity for education, networking, and celebrating Histotechnology. Hope to see you on the island! Wanda K. Simons HT(ASCP) GSH President Augusta State University and Georgia Health Sciences University have consolidated to become Georgia Regents University. Effective January 9, 2013, my email address has changed to BZIMMERM@gru.edu. Please update your address book to reflect this change. From jpiche-grocki <@t> wtbyhosp.org Fri Feb 8 13:16:30 2013 From: jpiche-grocki <@t> wtbyhosp.org (PicheGrocki, Jessica) Date: Fri Feb 8 13:16:37 2013 Subject: [Histonet] CYP 04300 Message-ID: <631955447A364B45B9458D2905635110655C3954@WIN08-MBX-01.wtbyhosp.org> Hi All, I have a question from our Cytology department. For question CYP 04300, how is everyone managing their QC slides for cytology stains? Are you doing separate QC slides for thin preps, smears, FNA's etc? Thanks, Jessica Piche' HT(ASCP) Waterbury Hospital CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital From joelleweaver <@t> hotmail.com Fri Feb 8 14:04:10 2013 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Fri Feb 8 14:04:16 2013 Subject: [Histonet] What's on that H&E? In-Reply-To: References: Message-ID: Just depends on the protocol at that laboratory- often at the discretion of the pathologist(s) reading or the medical director- I guess all variations are acceptable so long as all the sections are of good quality, they may not want/need serials, but prefer levels, and if they meet the diagnostic needs, and are representative of the tissue sample. I think you could do things a lot of different ways dependant on the tissue type and clinical differential. I have seen and done many of the variations you mention in your post, up to 9-12 serial sections ( rows of 3 ribbons on one slide) and up to 12 levels ( specific depth into the block) on single or multiple slides., with special stains and IHC levels thrown in as well- so "sky's the limit" to me as to what might work for your lab and pathologists. Sometimes you don't really need to provide all the extra sections, but for some specimens that might make sense. So I like it when the protocols are pretty specific- though that variability may not apply to your lab if you do only a few tissue types. Anyhow I would rather do all that is likely to be needed the first time into the block, no matter how complicated the protocol, than have to recut it again and again. You might end up losing too much tissue that you need later for molecular or other testing too. Outside numbers are a good initial benchmark to me, but your own productivity statistics and staff are often a lot more meaningful. I have just seen different numbers for rates on these protocols versus single section slides. Maybe do a side by side, of having them read single section slides and then the same specimen type ( as similar as possible) with other different sectioning using complex protocols with times to produce in sectioning time, stain throughput, all variables you can think of- maybe they may have different opinions then? Just an idea. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: BGapinski@pathgroup.com > To: histonet@lists.utsouthwestern.edu > Date: Fri, 8 Feb 2013 18:00:11 +0000 > Subject: [Histonet] What's on that H&E? > > Dear Histonians, > How many sections do put on a slide from a block cast in a small embedding mold? Do you automatically include any levels? Then please tell me how many slides your techs cut per hour? > The problem is (may be) that we put 4 levels on a slide with two sections per level. That is: > > 1. Full face (or pretty close) 2 sections > 2. Level 2 sections > 3. Level 2 sections > 4. Level 2 sections > So I have 8 sections on my H&E. This takes me some time, and I understand all histologists may not be doing things this way. But the problem is, when we discuss output and my techs appear slow I find that other labs put much less tissue on a slide. Some labs give a fatty string of serial sections in two rows, and that appears to be just like my slide. But only on the surface (pardon the pun). > My pathologists just renegotiated the contracts for all our dermatologists after the new 88305TC pricing, and those doctors say "Oh, we can get slides cheaper than that." Maybe they can but what would they see on the H&E, and would they care? > So now I'm faced with doing things as we have or stop giving those levels. I understand my job as a Histologist is to demonstrate the tissue. So I'll stick to the levels, because I: > > 1. Work for the patient > > 2. Am supervised by the Pathologists > > 3. Am reimbursed by our company > Tell me what you do in you lab, please > > Bruce Gapinsk HT (ASCP) > Chief Histologist > Marin Medical Laboratories > PathGroup SF > > > ________________________________ > > Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Fri Feb 8 14:10:56 2013 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Fri Feb 8 14:11:01 2013 Subject: [Histonet] Could these samples have been saved? In-Reply-To: <9302f92021acbe8e570f36e8cfb283ad.squirrel@webmail.rci.rutgers.edu> References: <59F7809368E0084C8AE6B38C93D735EB2FA103F52A@MEDEXMB02.ad.med.miami.edu>, <9302f92021acbe8e570f36e8cfb283ad.squirrel@webmail.rci.rutgers.edu> Message-ID: It was my understanding that with MW-assited processing, that they could not be put back through the MW assisted fixation part of the program. That is not to say that you couldn't rehydrate tissues and then evaluate and pass them through the rest if they were fixed, such as the alcohols, isopropanol and/or paraffin. I supposed you could conventionally fix if that were the issue, but I do remember when using these instruments that I didn't "double microwave". Instead, I just made an edited program to take it to wax depending on the situation. Most of the software made this pretty easy to do. Does anyone know differently? Joelle Weaver MAOM, HTL (ASCP) QIHC > Date: Fri, 8 Feb 2013 13:22:15 -0500 > From: kgrobert@rci.rutgers.edu > To: KJohnson@med.miami.edu > Subject: Re: [Histonet] Could these samples have been saved? > CC: histonet@lists.utsouthwestern.edu > > I don't know about your samples now, but for future reference, you might > find this interesting: > http://realhistoryww.com/world_history/ancient/Misc/Data/Study_mummified_soft_tissues.htm > > Kathleen Roberts > > Principal Lab Technician > Neurotoxicology Labs > Molecular Pathology Facility Core > Dept of Pharmacology & Toxicology > Rutgers, the State University of NJ > 41 B Gordon Road > Piscataway, NJ 08854 > (848) 445-1443 > FAX (732) 445-6905 > > > Dear all, > > > > During an overnight tissue processing cycle, a malfunction occurred such > > that the sample basket was suspended in mid-air for several hours at > > probably the worst spot in which to do so---after the final absolute > > ethanol of the dehydration series. I continued the process manually in the > > morning, and carried it through blocking and attempted sectioning. > > However, the samples (mouse skin and fat) had been converted to uncuttable > > rocks. > > > > In hindsight, should I have attempted to rehydrate and reprocess these > > samples in an attempt to glean even minimal information from them? Or is > > there no way to unmummify a mummy? > > > > Regards, > > > > Kevin Johnson > > University of Miami > > Diabetes Research Institute > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Virginia.Chladek <@t> flhosp.org Fri Feb 8 14:13:06 2013 From: Virginia.Chladek <@t> flhosp.org (Chladek, Virginia) Date: Fri Feb 8 14:16:47 2013 Subject: [Histonet] Temps In-Reply-To: <02.95.02937.E5D35115@mx1.fhmis.net> References: <02.95.02937.E5D35115@mx1.fhmis.net> Message-ID: RE: temperatures We press reset on the thermometers on Friday, then record the min and max temps on the Monday after. As long as those are within range then we are ok... Virginia Chladek, HTL FL Message: 1 Date: Fri, 8 Feb 2013 15:32:22 +0000 From: "Sullivan, Beatrice" Subject: RE: [Histonet] Temperatures To: Rene J Buesa , "histonet@lists.utsouthwestern.edu" Message-ID: <6932520047F7EE46B512E9801344F1600467CC@ExchangeMB-1.Virtua.org> Content-Type: text/plain; charset="utf-8" Because I have been told that according to JCAHO (who will be inspecting us) that if a temperature is required to ascertain consistency, quality and integrity, you will have to record temperature 7 days per week. , This message (including any attachments) is intended only for the use of the individual or entity to which it is addressed and may contain information that is non-public, proprietary, privileged, confidential, and exempt from disclosure under applicable law or may constitute as attorney work product. If you are not the intended recipient, you are hereby notified that any use, dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, notify us immediately by telephone and (i) destroy this message if a facsimile or (ii) delete this message immediately if this is an electronic communication. Thank you. From rjbuesa <@t> yahoo.com Fri Feb 8 14:55:15 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Feb 8 14:55:21 2013 Subject: [Histonet] Could these samples have been saved? In-Reply-To: <59F7809368E0084C8AE6B38C93D735EB2FA103F52A@MEDEXMB02.ad.med.miami.edu> References: <59F7809368E0084C8AE6B38C93D735EB2FA103F52A@MEDEXMB02.ad.med.miami.edu> Message-ID: <1360356915.4151.YahooMailNeo@web163106.mail.bf1.yahoo.com> You are right. You should have tried to rehydrate the tissues, especially mouse that is very lean in itself. You cannot unmummify them now. Ren? J. From: "Johnson, Kevin" To: "'histonet@lists.utsouthwestern.edu'" Sent: Friday, February 8, 2013 1:00 PM Subject: [Histonet] Could these samples have been saved? Dear all, During an overnight tissue processing cycle, a malfunction occurred such that the sample basket was suspended in mid-air for several hours at probably the worst spot in which to do so---after the final absolute ethanol of the dehydration series. I continued the process manually in the morning, and carried it through blocking and attempted sectioning.? However, the samples (mouse skin and fat) had been converted to uncuttable rocks. In hindsight, should I have attempted to rehydrate and reprocess these samples in an attempt to glean even minimal information from them?? Or is there no way to unmummify a mummy? Regards, Kevin Johnson University of Miami Diabetes Research Institute _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kiran_g <@t> sbcglobal.net Fri Feb 8 16:31:44 2013 From: kiran_g <@t> sbcglobal.net (Kiranjit Grewal) Date: Fri Feb 8 16:31:49 2013 Subject: [Histonet] Histology QA Manager opening at Kaiser Berkeley, CA Message-ID: <1360362704.73609.YahooMailClassic@web184405.mail.bf1.yahoo.com> Hi Histonetters, ? Great opportunity to work for one of the best Histology Lab. Excellent benefits and tremendous growth opportunities in the organization. ? Here is the posting and please contact me if you have any questions or apply on line. ? Regional Lab QA Section Manager, Histology [BRK067](?Job #?154196) ? Description? Under the general direction of the Laboratory Medical Director and the immediate supervision of the Pathology Director and Director of Laboratory Services, Quality and collaboration with Histology operational managers, NCAL pathology departments and Regional Laboratory Histology Director, directs and controls the Histology departmental specific Laboratory Quality System/Assessment program to improve quality, services, and meet all regulatory requirements. ? Essential Functions: ? Manages all histology activities, including recruiting, hiring and training of staff. ? Ensures competency, motivates and encourages professional growth. ? Controls costs by monitoring productivity, personnel utilization, overtime, material usage rates, analyzing fluctuations in types and volumes of tests, and implements corrective actions. ? Participates in the design of Regional Laboratory and Northern California Region integrated laboratory quality system.? ? Ensures compliance with regulatory and accreditation agencies' rules and regulations. ?? Designs and implements effective risk control processes. ? Leads in the research of new and/or improved test development methodologies by:? performing experimental testing procedures;? validating effectiveness/feasibility for implementation;? cost of procedure(s);? preparing and submitting recommendation(s) for change to laboratory management and other stakeholders (such as Chiefs of Pathologists). ? Researches and resolves client problem/issues. ? Oversees and coordinates startup and implementation activities resulting from new services or transfer of services. ? Participates in department, inter-department, inter-facility, and inter-regional level projects which help the regional laboratory achieve its goal of providing quality service and client support in a cost effective manner. ?? Develops transition plan for new services/tests, outreach programs for transfer of work, timelines, and monitors milestones to achieve service expectations. ?? Coordinates internal resources to support new service. ? Serves as primary liaison to RILIS/ITS for ongoing and new issues.? ? Develops needs analysis as appropriate.? Leads in the integration of secondary laboratory information system with RILIS. ? Kaiser Permanente conducts compensation reviews of positions on a routine basis. ?? At any time, Kaiser Permanente reserves the right to reevaluate and change job descriptions, or to change such positions from salaried to hourly pay status.? ? Such changes are generally implemented only after notice is given to affected employees. ? Qualifications? Basic Qualifications: ? Significant experience in high-volume histology laboratory required (usually five years). ? Previous supervisory/managerial experience (usually three years).? ? Bachelor's in biological sciences or related field required. ? Master's in science or related field preferred. ? Additional courses in business administration and/or management preferred. ? Certification by the American Society for Clinical Pathologists. ? Must be able to work in a Labor/Management Partnership environment. ? Preferred Qualifications: ??Minimum 5 years experience in a high volume clinical laboratory or complex healthcare delivery system. ??At least 3 years in recent progressive supervisory/management responsibilities. ??Proven strong leadership and human resource skills. ??Demonstrable skills and experience in creating a collaborative work environment and labor management partnership. ??Demonstrable experience in project management, quality system management, process mapping, and regulatory compliance. ??Demonstrable experience in high level of initiative, good judgment, exemplary interpersonal skills, excellent written/verbal communication skills, excellent presentation, and supporting all levels of internal and external clients in a multifaceted, culturally diverse, and complex operation. ??Demonstrable knowledge and/or skills in budgeting, purchasing, staffing, scheduling, and employee coaching/mentoring. ??Knowledge in bargaining union contract applications. Proficient skills in using various computer applications in document creation, statistical analysis, graphic display, and flowcharting.??Ability to be?flexible in schedules to meet deadlines or operational requirements as needed.? Primary Location :?California-Berkeley-Berkeley Regional Lab - 1725 1725 Eastshore? Scheduled Hours (1-40) :?40? Shift :?Day? Working Days :?Mon - Fri? Working Hours Start :?9:00AM? Working Hours End :?5:30PM? ? ? Kiranjit Grewal ? From amosbrooks <@t> gmail.com Fri Feb 8 19:03:17 2013 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Fri Feb 8 19:03:27 2013 Subject: [Histonet] Temperatures Message-ID: Good Grief! Why would this really be an issue. The temperatures are taken throughout the week and are constant (or you have a different problem entirely) why would they only spike or tank on the weekend, and why would it even matter if the equipment isn't being used. If a tree falls in the forest and no one is around to hear it who the heck cares? Face-palm, Amos On Fri, Feb 8, 2013 at 10:17 AM, wrote: > Message: 16 > Date: Fri, 8 Feb 2013 12:52:29 +0000 > From: "Sullivan, Beatrice" > Subject: [Histonet] Temperatures > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > <6932520047F7EE46B512E9801344F16004674B@ExchangeMB-1.Virtua.org> > Content-Type: text/plain; charset="us-ascii" > > I'm looking for a fix to our problem of no temperatures being taken on the > weekends. We are closed and this is creating an issue. Our processors are > not running until Sunday night but the paraffin in both the processors and > embedding center are kept molten. Any help would be greatly appreciated > > Beatrice L. Sullivan HT(ASCP)HTL > Corporate Histology Manager > Virtua, Voorhees > 856-247-3144 > From Maxim_71 <@t> mail.ru Fri Feb 8 23:20:18 2013 From: Maxim_71 <@t> mail.ru (Maxim Peshkov) Date: Fri Feb 8 23:20:18 2013 Subject: [Histonet] Could these samples have been saved? Message-ID: <303651520.20130209092018@mail.ru> Kevin! We have had a similar problem, when our tissues totally dried on air during more than 18 hours after acetone and looked as a stones. We put our bloks into Luna's solution for repocessing tissue with formaldehyde, sodium acetate and glycerine (Laboratory methods in histotechnology, AFIP, 1992) and did as described in this chapter. It works great. We successfully sectioned all the blocks and have had accetable slides for diagnosis. Some years after we started to use mineral oil and isopropanol for processing. Isopropanol will not such severe drying tissues as acetone and ethanol. Maxim Peshkov, Russia, Taganrog. Original message--- From: "Johnson, Kevin" To: "'histonet@lists.utsouthwestern.edu'" Sent: Friday, February 8, 2013 1:00 PM Subject: [Histonet] Could these samples have been saved? Dear all, During an overnight tissue processing cycle, a malfunction occurred such that the sample basket was suspended in mid-air for several hours at probably the worst spot in which to do so---after the final absolute ethanol of the dehydration series. I continued the process manually in the morning, and carried it through blocking and attempted sectioning. However, the samples (mouse skin and fat) had been converted to uncuttable rocks. In hindsight, should I have attempted to rehydrate and reprocess these samples in an attempt to glean even minimal information from them?? Or is there no way to unmummify a mummy? Regards, Kevin Johnson University of Miami Diabetes Research Institute _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ mailto:Maxim_71@mail.ru From wdesalvo.cac <@t> outlook.com Fri Feb 8 23:20:55 2013 From: wdesalvo.cac <@t> outlook.com (WILLIAM DESALVO) Date: Fri Feb 8 23:21:00 2013 Subject: [Histonet] Temperatures In-Reply-To: References: Message-ID: Who cares? The patient. I am quite sure the patient wants quality assured results. Would any of us use a reagent or chemical from a manufacturer that could not prove the quality? If the instrument remains on during periods on inactivity in the lab, reagents or chemicals are stored in the the lab during the same periods, then you must monitor and prove the quality. It's kind of like, when we aren't around and the tree falls, we still need to know when is fell and if it fell on anything. Everything we do starts w/ standardization and because we produce patient test results, we must prove our processes continually meet the standard, good quality control and assurance. The accrediting and licensing organizations are just doing their part to check that we can, do and will only produce quality work, no exceptions. As has been previously mentioned, there are several solutions to the problem. If the lab is open, have someone from another area check, record and sign for the process. There are several electronic thermometers, w/ a probe, that can be set for a minimum and maximum range and accurately record temps during periods when staff is not available. We should never wait until we have a questionable result before we check for quality. William DeSalvo, BS HTL(ASCP) Production Manager-Anatomic Pathology Chair, NSH Quality Management Committee Owner/Consultant, Collaborative Advantage Consulting > Date: Fri, 8 Feb 2013 20:03:17 -0500 > From: amosbrooks@gmail.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Temperatures > > Good Grief! > Why would this really be an issue. The temperatures are taken > throughout the week and are constant (or you have a different problem > entirely) why would they only spike or tank on the weekend, and why would > it even matter if the equipment isn't being used. If a tree falls in the > forest and no one is around to hear it who the heck cares? > > Face-palm, > Amos > > From jnocito <@t> satx.rr.com Sat Feb 9 09:43:48 2013 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Sat Feb 9 09:50:52 2013 Subject: [Histonet] Unsubscribe Message-ID: <004d01ce06dc$40a3ee20$c1ebca60$@rr.com> Unsubscribe Joe Nocito, BS, PACM, HTCM (ASCP) QIHC Dept of Pathology/ 59LSQ/SGVLH Lackland AFB, TX 78236 joseph.nocito@us.af.mil From rjbuesa <@t> yahoo.com Sat Feb 9 11:53:31 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Feb 9 11:53:39 2013 Subject: [Histonet] Unsubscribe In-Reply-To: <004d01ce06dc$40a3ee20$c1ebca60$@rr.com> References: <004d01ce06dc$40a3ee20$c1ebca60$@rr.com> Message-ID: <1360432411.60363.YahooMailNeo@web163104.mail.bf1.yahoo.com> Joe, not you also!!!!!!!!!!!! You should know how to unsubscribe, you are not new to this website. Good luck in what ever you are going to do now. Ren? J. From: Joe Nocito To: histonet@lists.utsouthwestern.edu Sent: Saturday, February 9, 2013 10:43 AM Subject: [Histonet] Unsubscribe Unsubscribe Joe Nocito, BS, PACM, HTCM (ASCP) QIHC Dept of Pathology/ 59LSQ/SGVLH Lackland AFB, TX 78236 joseph.nocito@us.af.mil _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From madary <@t> verizon.net Sat Feb 9 16:11:47 2013 From: madary <@t> verizon.net (madary@verizon.net) Date: Sat Feb 9 16:12:06 2013 Subject: [Histonet] tissue dry out after alcohol Message-ID: <4142818.765872.1360447907107.JavaMail.root@vznit170182> I seem to recall using a combination of formalin and glycerin with the appropriate removal of the glycerin. I softens the tissue up and may not be the best to work with but might provide enough info for a decent path to diagnose. It is in AFIP green manual. Nick(Rocky) Madary, HT/HTL(ASCP)QIHC From jaylundgren <@t> gmail.com Sun Feb 10 11:15:29 2013 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Sun Feb 10 11:15:34 2013 Subject: [Histonet] Unsubscribe In-Reply-To: <1360432411.60363.YahooMailNeo@web163104.mail.bf1.yahoo.com> References: <004d01ce06dc$40a3ee20$c1ebca60$@rr.com> <1360432411.60363.YahooMailNeo@web163104.mail.bf1.yahoo.com> Message-ID: What does PACM or HTCM stand for? I couldn't find them on the ASCP site? High Throughput Combinatorial Microscopy? Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) On Sat, Feb 9, 2013 at 12:53 PM, Rene J Buesa wrote: > Joe, not you also!!!!!!!!!!!! > You should know how to unsubscribe, you are not new to this website. > Good luck in what ever you are going to do now. > Ren? J. > > From: Joe Nocito > To: histonet@lists.utsouthwestern.edu > Sent: Saturday, February 9, 2013 10:43 AM > Subject: [Histonet] Unsubscribe > > Unsubscribe > > > > Joe Nocito, BS, PACM, HTCM (ASCP) QIHC > > Dept of Pathology/ 59LSQ/SGVLH > > Lackland AFB, TX 78236 > > joseph.nocito@us.af.mil > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From amosbrooks <@t> gmail.com Sun Feb 10 14:31:14 2013 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Sun Feb 10 14:31:31 2013 Subject: [Histonet] Temperatures Message-ID: OK, Perhaps that was a fairly punchy and poorly thought out reply. I blame it on a day full of shoveling 2.5 feet of snow. Of course reagents should be monitored, and a temperature monitor would be cheaper than paying a tech just to go in and record them. I get titchy about what I consider unnecessary regulation though. Waterbath temperatures for example are just silly. If the sections don't expand turn it up, if they blow up turn it down. Amos On Sat, Feb 9, 2013 at 1:00 PM, wrote: > Message: 2 > Date: Fri, 8 Feb 2013 22:20:55 -0700 > From: WILLIAM DESALVO > Subject: RE: [Histonet] Temperatures > To: Amos Brooks , histonet > > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > Who cares? The patient. I am quite sure the patient wants quality assured > results. Would any of us use a reagent or chemical from a manufacturer that > could not prove the quality? If the instrument remains on during periods on > inactivity in the lab, reagents or chemicals are stored in the the lab > during the same periods, then you must monitor and prove the quality. It's > kind of like, when we aren't around and the tree falls, we still need to > know when is fell and if it fell on anything. Everything we do starts w/ > standardization and because we produce patient test results, we must prove > our processes continually meet the standard, good quality control and > assurance. The accrediting and licensing organizations are just doing their > part to check that we can, do and will only produce quality work, no > exceptions. As has been previously mentioned, there are several solutions > to the problem. If the lab is open, have someone from another area check, > record and sign for the process. There are several electronic thermometers, > w/ a probe, that can be set for a minimum and maximum range and accurately > record temps during periods when staff is not available. We should never > wait until we have a questionable result before we check for quality. > William DeSalvo, BS HTL(ASCP) > Production Manager-Anatomic Pathology > Chair, NSH Quality Management Committee > Owner/Consultant, Collaborative Advantage Consulting > From debgranato <@t> yahoo.com Sun Feb 10 14:49:58 2013 From: debgranato <@t> yahoo.com (Debbie Granato) Date: Sun Feb 10 14:50:02 2013 Subject: [Histonet] Microwave Procedure for Prostate Needle Biopsies Message-ID: <1360529398.59001.YahooMailClassic@web161204.mail.bf1.yahoo.com> Does anyone have a procedure for microwave processing prostate needle biopsies fixed in formalin? Does microwave processing ever affect IHC staining? I appreciate your help! Debbie Granato HT(ASCP) From Dorothy.L.Webb <@t> HealthPartners.Com Mon Feb 11 09:09:17 2013 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Mon Feb 11 09:09:31 2013 Subject: [Histonet] troubleshooting help Message-ID: <65365F35C0F2EF4D846EC3CA73E49C430227AFFBCCC7@HPEMX3.HealthPartners.int> I am having trouble with this scenario that I have walked into today. Our Sakura VIP 5 tissue processor had about 150 cassettes on it and they are all overprocessed, very hard. I have checked with a hydrometer all of the alcohols and all seem fine and in the proper order. There were no error codes, nothing stopped in any given step, they were done on time. The only thing that is unusual is that when the tech opened up the processor, she noticed the top of the lid had a large amount of paraffin hanging from it, which we do not normally see. I checked the carbon filter, it is fine. Anybody out there have any ideas as to what could be going on ????? Much thanks for whatever advice I am given. I have never in all of my years come across a scenario such as this without there having been alcohols incorrectly placed. Dorothy Webb, HT (ASCP) Regions Histology Technical Specialist 651-254-2962 ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 From campbellj <@t> muhlbauerlab.com Mon Feb 11 09:26:02 2013 From: campbellj <@t> muhlbauerlab.com (Jennifer Campbell) Date: Mon Feb 11 09:26:06 2013 Subject: [Histonet] troubleshooting help In-Reply-To: <65365F35C0F2EF4D846EC3CA73E49C430227AFFBCCC7@HPEMX3.HealthPartners.int> References: <65365F35C0F2EF4D846EC3CA73E49C430227AFFBCCC7@HPEMX3.HealthPartners.int> Message-ID: We have seen that if our paraffins were not properly rotated. On Mon, Feb 11, 2013 at 10:09 AM, Webb, Dorothy L < Dorothy.L.Webb@healthpartners.com> wrote: > I am having trouble with this scenario that I have walked into today. Our > Sakura VIP 5 tissue processor had about 150 cassettes on it and they are > all overprocessed, very hard. I have checked with a hydrometer all of the > alcohols and all seem fine and in the proper order. There were no error > codes, nothing stopped in any given step, they were done on time. The only > thing that is unusual is that when the tech opened up the processor, she > noticed the top of the lid had a large amount of paraffin hanging from it, > which we do not normally see. I checked the carbon filter, it is fine. > > Anybody out there have any ideas as to what could be going on ????? Much > thanks for whatever advice I am given. I have never in all of my years > come across a scenario such as this without there having been alcohols > incorrectly placed. > > Dorothy Webb, HT (ASCP) > Regions Histology Technical Specialist > 651-254-2962 > > > > ________________________________ > This e-mail and any files transmitted with it are confidential and are > intended solely for the use of the individual or entity to whom they are > addressed. If you are not the intended recipient or the individual > responsible for delivering the e-mail to the intended recipient, please be > advised that you have received this e-mail in error and that any use, > dissemination, forwarding, printing, or copying of this e-mail is strictly > prohibited. > > If you have received this communication in error, please return it to the > sender immediately and delete the original message and any copy of it from > your computer system. If you have any questions concerning this message, > please contact the sender. Disclaimer R001.0 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Jen Campbell, BS, HT(ASCP) Supervisor of Technical Services Muhlbauer Dermatopathology Laboratory 61 Monroe Avenue, Ste B Pittsford NY 14534 P: 585.586.5166 F: 585.586.3137 IMPORTANT NOTICE: This e-mail and any attachments may contain confidential or sensitive information which is, or may be, legally privileged or otherwise protected by law from further disclosure. It is intended only for the addressee. If you received this in error or from someone who was not authorized to send it to you, please do not distribute, copy or use it or any attachments. Please notify the sender immediately by reply e-mail and delete this from your system. Thank you for your cooperation. From rjbuesa <@t> yahoo.com Mon Feb 11 09:26:04 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Feb 11 09:26:09 2013 Subject: [Histonet] Microwave Procedure for Prostate Needle Biopsies In-Reply-To: <1360529398.59001.YahooMailClassic@web161204.mail.bf1.yahoo.com> References: <1360529398.59001.YahooMailClassic@web161204.mail.bf1.yahoo.com> Message-ID: <1360596364.46800.YahooMailNeo@web163101.mail.bf1.yahoo.com> The protocol will have a lot to do with the processor you have, because not all have the same power in their tube, so you will be better off by asking the manufacturer of your MW oven for a standard protocol they recommend. As to your second question MW ovens never affect the results of IHC procedures, but they will be affected by improper fixation. Ren? J. From: Debbie Granato To: histonet@lists.utsouthwestern.edu Sent: Sunday, February 10, 2013 3:49 PM Subject: [Histonet] Microwave Procedure for Prostate Needle Biopsies Does anyone have a procedure for microwave processing prostate needle biopsies fixed in formalin? Does microwave processing ever affect IHC staining? I appreciate your help! Debbie Granato HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cmiller <@t> physlab.com Mon Feb 11 09:45:35 2013 From: cmiller <@t> physlab.com (Cheri Miller) Date: Mon Feb 11 09:45:41 2013 Subject: [Histonet] Fungus Control Tissue Message-ID: I need a new Fungus control block. Can anyone spare some? Thanks. Cheri Cheryl A. Miller HT(ASCP)cm Histology Supervisor, Hygiene Officer Physicians Laboratory Services 4840 F Street Omaha, NE. 68127-0999 402 731 4145 ext. 554 ________________________________ PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From rheyna <@t> lumc.edu Mon Feb 11 09:57:00 2013 From: rheyna <@t> lumc.edu (Roger Heyna) Date: Mon Feb 11 09:57:17 2013 Subject: [Histonet] Leica Bond III Opinions In-Reply-To: References: Message-ID: <5118C06C020000230004A0E3@gwgwia1.luhs.org> Forwarding to Histonet: Hi Roger, The Bond IHC unit is used in two of the laboratories where I work. My hands-on experience is limited since I'm the new kid on the block but here's what I've learned, from my mistakes. The unit does not know if the covertiles are in place or not and the staining is awful without them. I don't think we have come up with an efficient way to effectively clean the covertiles. Rinsing or soaking in alcohol just doesn't do a very good job. I have spent way too much time soaking, wiping, and then rinsing. No broken slide trays yet so they are holding up to daily use. I know the pathologists are happy with the stain results. The techs are happy with the ease of use. Shorter run time was one of the things used to justify purchasing the Bond. The "open" system allows for use of antibodies not from Leica. I was not involved in the validation process so I don't know the specifics of choosing CellMarque or some of the other suppliers and for some reason OSCAR and one or two other protocols are still run on an older Ventana unit. The Bond requires a fair amount of daily and weekly maintenance but I think that's the norm for the more sophisticated stainers. These are my observations from one unit, in one lab. The other laboratory where I cut paraffin blocks a couple nights a week, has six Bonds of various generations. The only thing I can say about them is they must get the job done or there would not have been such a big investment. Take care, Dorothy In a message dated 2/6/2013 2:16:19 P.M. Eastern Standard Time, rheyna@lumc.edu writes: Could I get the opinions of any labs using the Leica Bond III for IHC staining? I'm especially interested to hear from those that switched from Ventana to Leica, but any feedback is appreciated. Few questions that come to mind: Are you happy with the quality of the stains you're performing on the Bond III? Are there antibodies that you were not able to run on the Bond III? Is the machine reliable, or does it break down often, requiring technical service? How arduous is cleaning and managing the cover tiles? Are the three black slide drawers durable? Thank you, Roger Heyna Maywood, IL _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 <@t> earthlink.net Mon Feb 11 11:04:28 2013 From: relia1 <@t> earthlink.net (Pam Barker) Date: Mon Feb 11 11:04:35 2013 Subject: [Histonet] RELIA Hot Job Alert Molecular Diagnostics Supervisor needed in Springfield, OR Message-ID: <011e01ce0879$db57ea40$9207bec0$@earthlink.net> Hi Histonetters!! I hope your week is getting off to a great start!! I have an exciting new opportunity that I want to share. Molecular Diagnostics Lab Supervisor - Springfield, OR RELIA Solutions is working with a NW based Hospital system in their search for a Supervisor for their Molecular Diagnostics Laboratories. This is a full time permanent position managing 2 labs. Molecular Diagnostics experience and strong supervisory experience. This is an exciting opportunity to participate in the administration planning and continued development of this growing Molecular department. ASCP MT, MLT, HT, HTL or CT is required. Our client offers an excellent salary, great benefits and relocation assistance. If this is the right job for you RELIA can make it happen! For more information please contact Pam Barker at relia1@earthlink.net or toll free at 866-607-3542 RELIA Solutions is the nation's ONLY recruiting firm specializing in the nationwide permanent placement of histology professionals. To sign up for our free histology careers bulletin please send an e-mail to relia1@earthlink.net and include subscribe in the subject line. Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From rsrichmond <@t> gmail.com Mon Feb 11 12:20:00 2013 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Mon Feb 11 12:20:04 2013 Subject: [Histonet] Re: Microwave Procedure for Prostate Needle Biopsies Message-ID: Debbie Granato HT(ASCP) asks >>Does anyone have a procedure for microwave processing prostate needle biopsies fixed in formalin? Does microwave processing ever affect IHC staining?<< Do your pathologists have any input into the decision to microwave prostate biopsy specimens? I'd be concerned about the effect of microwaving on immunohistochemistry, I'd be concerned about what the regulatory agencies are telling us to do, and I'd want to know what the big commercial prostate labs are doing. Bob Richmond Samurai Pathologist Maryville TN From Donna.Willis <@t> baylorhealth.edu Mon Feb 11 13:10:20 2013 From: Donna.Willis <@t> baylorhealth.edu (Willis, Donna G.) Date: Mon Feb 11 13:11:11 2013 Subject: FW: [Histonet] Re: Microwave Procedure for Prostate Needle Biopsies Message-ID: <2572B4D63B62E64A8078D8BBE34D407802CB97@BHDASVEXML2.bhcs.pvt> Dr. Richmond. There is no effect on IHC with microwave processed tissue. Regulatory agencies (CAP and CLIA) are not telling us anything other than Validate. OUR lab (Dr. Oppenheimer) uses microwaves for processing. Donna Willis, HT/HTL (ASCP) Anatomic Pathology Manager Baylor University Medical Center-Dallas ph. 214-820-2465 office ph. 214-725-6184 mobile donna.willis@baylorhealth.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Monday, February 11, 2013 12:20 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Microwave Procedure for Prostate Needle Biopsies Debbie Granato HT(ASCP) asks >>Does anyone have a procedure for microwave processing prostate needle biopsies fixed in formalin? Does microwave processing ever affect IHC staining?<< Do your pathologists have any input into the decision to microwave prostate biopsy specimens? I'd be concerned about the effect of microwaving on immunohistochemistry, I'd be concerned about what the regulatory agencies are telling us to do, and I'd want to know what the big commercial prostate labs are doing. Bob Richmond Samurai Pathologist Maryville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This e-mail may contain confidential and/or privileged information. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. From Timothy.Morken <@t> ucsfmedctr.org Mon Feb 11 14:42:34 2013 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Mon Feb 11 14:42:44 2013 Subject: [Histonet] Something different! Message-ID: <761E2B5697F795489C8710BCC72141FF054A69@ex07.net.ucsf.edu> Here is something I found while searching for something histology related: A slide stainer for the SkyLab space station from 1972!! http://ntrs.nasa.gov/archive/nasa/casi.ntrs.nasa.gov/19720014805_1972014805.pdf Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center From John.Garratt <@t> vch.ca Mon Feb 11 15:28:25 2013 From: John.Garratt <@t> vch.ca (Garratt, John [NS]) Date: Mon Feb 11 15:28:32 2013 Subject: [Histonet] Symposium in IHC and Molecular Diagnostics- April 2013 In-Reply-To: <20130211180431.A17C64315@relay-ex2.vch.ca> References: <20130211180431.A17C64315@relay-ex2.vch.ca> Message-ID: For more information and to register go to http://ciqc-capconference.eventbrite.ca/ Topics include: MisMatch Repair proteins, Ki67 and the ethics of the use of tissues in quality control. International Speakers from four countries. I great opportunity to talk about the future of IHC and molecular diagnostics John Garratt Vancouver, Canada From CIngles <@t> uwhealth.org Mon Feb 11 15:43:04 2013 From: CIngles <@t> uwhealth.org (Ingles Claire) Date: Mon Feb 11 15:43:09 2013 Subject: [Histonet] RE: Something different! In-Reply-To: <761E2B5697F795489C8710BCC72141FF054A69@ex07.net.ucsf.edu> References: <761E2B5697F795489C8710BCC72141FF054A69@ex07.net.ucsf.edu> Message-ID: Seriously cool! Will have to try to read the whole thing in my spare time. The first few pages look intriguing already. Claire From cforster <@t> umn.edu Mon Feb 11 16:36:29 2013 From: cforster <@t> umn.edu (Colleen Forster) Date: Mon Feb 11 16:36:32 2013 Subject: [Histonet] Stain to ID eosinophils specifically Message-ID: <5119726D.7000509@umn.edu> Does anyone have access to this reference for me? I have a person looking to ID eosinophils in fat samples. I am looking for a stain that will make finding them easier....this reference was mentioned....any other suggestions? These are FFPE samples. Suggested Ref ex. LIllie 4th Ed. pp750. ref.Am.J.Clin.Path.55:283,1971.) Thanks in advance... Colleen L. Forster From Susan.Wert <@t> cchmc.org Mon Feb 11 16:44:33 2013 From: Susan.Wert <@t> cchmc.org (Wert, Susan (Susan Wert)) Date: Mon Feb 11 16:44:38 2013 Subject: [Histonet] Reichert FC4D cryo unit available Message-ID: <12F351B8515AD341B90AC3D5D7480C20259FCFA8@MCEXMB2.chmccorp.cchmc.org> We have a surplus Reichert FC4D cryo unit, including manual, control box, cryo pump, and a 35L LN2 dewar. Purchaser only pays shipping expenses. If you are interested, please email Dr. Cheng-Lun Na (Cheng-Lun.Na@CCHMC.org). _________________________________ Susan E. Wert, Ph.D. Associate Professor of Pediatrics University of Cincinnati College of Medicine Department of Pediatrics Director, Molecular Morphology Core Perinatal Institute Divisions of Neonatology, Perinatal and Pulmonary Biology Cincinnati Children's Hospital Medical Center 3333 Burnet Avenue, MLC7029 Cincinnati, Ohio 45229-3039 TEL: 513-636-4297 (office, voice mail) 513-636-3522 (laboratory) FAX: 513-636-7868 E-mail: Susan.Wert@CCHMC.org From Vickroy.Jim <@t> mhsil.com Mon Feb 11 16:53:22 2013 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Mon Feb 11 16:53:30 2013 Subject: [Histonet] Her2neu controls Message-ID: When we order the antibody from Ventana they include some control slides with tissues that are 0, 1+, 2+, and 3+. Currently the lab where we have sent our Her2neu's to be done only uses a 3+ control. I realize that a control that had several levels of staining would be ideal but I am trying to find out how other diagnostic labs are handling their controls for Her2neu. Thanks James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From Thomas.Jasper <@t> deaconess.com Tue Feb 12 08:59:59 2013 From: Thomas.Jasper <@t> deaconess.com (Thomas Jasper) Date: Tue Feb 12 09:00:07 2013 Subject: [Histonet] FW: Automated Microtome question Message-ID: See below. Thanks, tj ________________________________ From: Thomas Jasper Sent: Tuesday, February 12, 2013 8:56 AM To: 'histonet-request@lists.utsouthwestern.edu' Subject: Automated Microtome question Hi Everyone, Question - Does anyone know if there is a regulation in place (OSHA, i.e.) about having automated microtome(s) available to histologists if requested? In other words; I was under the impression that after 2005 (I think...) if someone wanted an automated microtome the institution they work at is mandated to provide it to them. This is not to say that folks must use an automated microtome; just that it's there or would be obtained for them. I thought I'd heard something about this a few years ago. With the repetitive motion issues today I was thinking this was a regulation of some sort. Thanks in advance for the input. Tom Jasper Thomas Jasper HT (ASCP) BAS AP/CP Supervisor Deaconess Hospital Evansville, IN This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Deaconess Health System. If you have received this email in error please notify the originator of the message. From relia1 <@t> earthlink.net Tue Feb 12 09:16:15 2013 From: relia1 <@t> earthlink.net (Pam Barker) Date: Tue Feb 12 09:16:19 2013 Subject: [Histonet] OT: Hi Histonetters!! I have some news I would like to share! Message-ID: <007201ce0933$e6821570$b3864050$@earthlink.net> Hi Histonetters!! I have some news I would like to share. We are celebrating the 8 year anniversary of RELIA Solutions for Histology Professionals. First I want to Thank all of you for taking the time to read and respond to my emails. I really appreciate it. We even got a little gift to celebrate the occasion: Hurray! I have one of the top 5% most viewed @LinkedIn profiles for 2012. http://www.linkedin.com/pub/profile/4/033/125 I would like to invite you to connect with me Pam Barker, on LinkedIN. Thanks and have a GREAT Day!! Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From histotech04 <@t> yahoo.com Tue Feb 12 09:41:14 2013 From: histotech04 <@t> yahoo.com (Elaine Dodds) Date: Tue Feb 12 09:41:22 2013 Subject: [Histonet] Looking for Histology job in NW Florida Message-ID: <1360683674.73403.YahooMailNeo@web161901.mail.bf1.yahoo.com> Hi all, I just moved to NW Florida and looking for a part/full time Histology position. I have 18 years experience and HT certified with a Florida license. Thank you in advance.. From jqb7 <@t> cdc.gov Tue Feb 12 09:52:18 2013 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/OID/NCEZID)) Date: Tue Feb 12 09:53:40 2013 Subject: [Histonet] RE: Automated Microtome question In-Reply-To: References: Message-ID: I would love to hear the info. on this so please share. Jeanine H. Bartlett Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 404-639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Thomas Jasper Sent: Tuesday, February 12, 2013 10:00 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] FW: Automated Microtome question See below. Thanks, tj ________________________________ From: Thomas Jasper Sent: Tuesday, February 12, 2013 8:56 AM To: 'histonet-request@lists.utsouthwestern.edu' Subject: Automated Microtome question Hi Everyone, Question - Does anyone know if there is a regulation in place (OSHA, i.e.) about having automated microtome(s) available to histologists if requested? In other words; I was under the impression that after 2005 (I think...) if someone wanted an automated microtome the institution they work at is mandated to provide it to them. This is not to say that folks must use an automated microtome; just that it's there or would be obtained for them. I thought I'd heard something about this a few years ago. With the repetitive motion issues today I was thinking this was a regulation of some sort. Thanks in advance for the input. Tom Jasper Thomas Jasper HT (ASCP) BAS AP/CP Supervisor Deaconess Hospital Evansville, IN This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Deaconess Health System. If you have received this email in error please notify the originator of the message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BDeBrosse-Serra <@t> isisph.com Tue Feb 12 09:55:42 2013 From: BDeBrosse-Serra <@t> isisph.com (Bea DeBrosse-Serra) Date: Tue Feb 12 09:55:58 2013 Subject: [Histonet] OT: Hi Histonetters!! I have some news I would like to share! In-Reply-To: <007201ce0933$e6821570$b3864050$@earthlink.net> References: <007201ce0933$e6821570$b3864050$@earthlink.net> Message-ID: <493CAA64F203E14E8823737B9EE0E25F092E5BA98B@EXCHMB01.isis.local> Happy anniversary! :) Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pam Barker Sent: Tuesday, February 12, 2013 7:16 AM To: Histonet Subject: [Histonet] OT: Hi Histonetters!! I have some news I would like to share! Hi Histonetters!! I have some news I would like to share. We are celebrating the 8 year anniversary of RELIA Solutions for Histology Professionals. First I want to Thank all of you for taking the time to read and respond to my emails. I really appreciate it. We even got a little gift to celebrate the occasion: Hurray! I have one of the top 5% most viewed @LinkedIn profiles for 2012. http://www.linkedin.com/pub/profile/4/033/125 I would like to invite you to connect with me Pam Barker, on LinkedIN. Thanks and have a GREAT Day!! Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Feb 12 10:45:22 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Feb 12 10:45:29 2013 Subject: [Histonet] FW: Automated Microtome question In-Reply-To: References: Message-ID: <1360687522.48035.YahooMailNeo@web163105.mail.bf1.yahoo.com> It is a documented fact that manual microtomes can produce, with time and more on certain histotechs than in others, a repetitive motion affection BUT there is no mandatory or compulsory legislation about purchasing "automated" microtomes to "prevent" the problem. That is an internal issue for each lab and its HRS departments. Usually histotechs with the affection are assigned tasks not requiring movements that can exacerbate their condition. Ren? J. ? From: Thomas Jasper To: "'histonet@lists.utsouthwestern.edu'" Sent: Tuesday, February 12, 2013 9:59 AM Subject: [Histonet] FW: Automated Microtome question See below. Thanks, tj ________________________________ From: Thomas Jasper Sent: Tuesday, February 12, 2013 8:56 AM To: 'histonet-request@lists.utsouthwestern.edu' Subject: Automated Microtome question Hi Everyone, Question - Does anyone know if there is a regulation in place (OSHA, i.e.) about having automated microtome(s) available to histologists if requested?? In other words; I was under the impression that after 2005 (I think...) if someone wanted an automated microtome the institution they work at is mandated to provide it to them. This is not to say that folks must use an automated microtome; just that it's there or would be obtained for them.? I thought I'd heard something about this a few years ago.? With the repetitive motion issues today I was thinking this was a regulation of some sort. Thanks in advance for the input. Tom Jasper Thomas Jasper HT (ASCP) BAS AP/CP Supervisor Deaconess Hospital Evansville, IN This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Deaconess Health System. If you have received this email in error please notify the originator of the message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Tue Feb 12 10:57:19 2013 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/OID/NCEZID)) Date: Tue Feb 12 10:57:38 2013 Subject: [Histonet] FW: Automated Microtome question In-Reply-To: <1360687522.48035.YahooMailNeo@web163105.mail.bf1.yahoo.com> References: <1360687522.48035.YahooMailNeo@web163105.mail.bf1.yahoo.com> Message-ID: The Microm Ergostar is a great option for those labs that cannot afford a fully automated microtome. I used one for years after my carpal tunnel surgery and highly recommend it. It is a rotary microtome but you have a push/pull handle instead of a wheel. And it is suitable for right or left-handed techs and completely adjustable. http://www.microm.de/Microm%20Homepage/html/hm_200_e.html Jeanine H. Bartlett Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 404-639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, February 12, 2013 11:45 AM To: Thomas Jasper; 'histonet@lists.utsouthwestern.edu' Subject: Re: [Histonet] FW: Automated Microtome question It is a documented fact that manual microtomes can produce, with time and more on certain histotechs than in others, a repetitive motion affection BUT there is no mandatory or compulsory legislation about purchasing "automated" microtomes to "prevent" the problem. That is an internal issue for each lab and its HRS departments. Usually histotechs with the affection are assigned tasks not requiring movements that can exacerbate their condition. Ren? J. ? From: Thomas Jasper To: "'histonet@lists.utsouthwestern.edu'" Sent: Tuesday, February 12, 2013 9:59 AM Subject: [Histonet] FW: Automated Microtome question See below. Thanks, tj ________________________________ From: Thomas Jasper Sent: Tuesday, February 12, 2013 8:56 AM To: 'histonet-request@lists.utsouthwestern.edu' Subject: Automated Microtome question Hi Everyone, Question - Does anyone know if there is a regulation in place (OSHA, i.e.) about having automated microtome(s) available to histologists if requested?? In other words; I was under the impression that after 2005 (I think...) if someone wanted an automated microtome the institution they work at is mandated to provide it to them. This is not to say that folks must use an automated microtome; just that it's there or would be obtained for them.? I thought I'd heard something about this a few years ago.? With the repetitive motion issues today I was thinking this was a regulation of some sort. Thanks in advance for the input. Tom Jasper Thomas Jasper HT (ASCP) BAS AP/CP Supervisor Deaconess Hospital Evansville, IN This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Deaconess Health System. If you have received this email in error please notify the originator of the message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BGapinski <@t> pathgroup.com Tue Feb 12 11:38:30 2013 From: BGapinski <@t> pathgroup.com (Bruce Gapinski) Date: Tue Feb 12 11:38:47 2013 Subject: [Histonet] grossing tools Message-ID: Histonians, I'm sure we are not the only histology lab that deals with thick grossed specimens. Has anyone tried the new gross tools by Sakura? Or anything else that can cut ONE nickel thick. Tired of reprocessing. Bruce Gapinsk HT (ASCP) Chief Histologist Marin Medical Laboratories PathGroup SF ________________________________ Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you From marktarango <@t> gmail.com Tue Feb 12 11:49:06 2013 From: marktarango <@t> gmail.com (Mark Tarango) Date: Tue Feb 12 11:49:10 2013 Subject: [Histonet] Her2neu controls In-Reply-To: References: Message-ID: Hi Jim, We run the control slide that Ventana sends (although not the best control) but we add a section from our tissue control block before staining. The tissue control block contains a 0+, weakly staining 1+, and a 3+. We use the ventana/tissue control slide as our batch control. This slide must be QC'd by a pathologist before any HER2 IHC is sent to pathologists for scoring. In addition, each patient slide has a section of the tissue control. We use a weakly staining 1+ in the tissue control to monitor any slight change in the staining. If the 1+ suddenly is a 0+, then we have a problem. If you were just running a 3+ control, you probably wouldn't notice the staining difference in the control. Mark T. On Mon, Feb 11, 2013 at 2:53 PM, Vickroy, Jim wrote: > When we order the antibody from Ventana they include some control slides > with tissues that are 0, 1+, 2+, and 3+. Currently the lab where we have > sent our Her2neu's to be done only uses a 3+ control. I realize that a > control that had several levels of staining would be ideal but I am trying > to find out how other diagnostic labs are handling their controls for > Her2neu. > Thanks > > > > > James Vickroy BS, HT(ASCP) > > Surgical and Autopsy Pathology Technical Supervisor > Memorial Medical Center > 217-788-4046 > > > ________________________________ > This message (including any attachments) contains confidential information > intended for a specific individual and purpose, and is protected by law. If > you are not the intended recipient, you should delete this message. Any > disclosure, copying, or distribution of this message, or the taking of any > action based on it, is strictly prohibited. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From rjbuesa <@t> yahoo.com Tue Feb 12 11:50:56 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Feb 12 11:51:01 2013 Subject: [Histonet] grossing tools In-Reply-To: References: Message-ID: <1360691456.75827.YahooMailNeo@web163101.mail.bf1.yahoo.com> Milestone has a very impressive (and good) array of dissection tools. Ren? J. From: Bruce Gapinski To: "histonet@lists.utsouthwestern.edu" Sent: Tuesday, February 12, 2013 12:38 PM Subject: [Histonet] grossing tools Histonians, ? ? ? ? ? ? ? ? I'm sure we are not the only histology lab that deals with thick grossed specimens. Has anyone tried the new gross tools by Sakura? Or anything else that can cut ONE nickel thick. Tired of reprocessing. Bruce Gapinsk HT (ASCP) Chief Histologist Marin Medical Laboratories PathGroup SF ________________________________ Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From estellamireles <@t> gmail.com Tue Feb 12 11:50:58 2013 From: estellamireles <@t> gmail.com (Stella Mireles) Date: Tue Feb 12 11:51:04 2013 Subject: [Histonet] Cassette Printer Info Needed Message-ID: Hello Everyone, The print head on the cassette printer needs to be replaced again. Informed that it will need to be replaced again in another 3 -4 months. This is something I am not looking forward to seeing repaired over and over again. This piece of equipment was made in 2010. Can anyone recommend the cassette writer that you are using ? Is this print head replacement normal with your unit ? Thanks Stella M-Walters From jqb7 <@t> cdc.gov Tue Feb 12 11:54:11 2013 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/OID/NCEZID)) Date: Tue Feb 12 11:54:23 2013 Subject: [Histonet] RE: grossing tools In-Reply-To: References: Message-ID: These are really wonderful IF you can talk your pathologists (or whoever is grossing) into using them. Jeanine H. Bartlett Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 404-639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bruce Gapinski Sent: Tuesday, February 12, 2013 12:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] grossing tools Histonians, I'm sure we are not the only histology lab that deals with thick grossed specimens. Has anyone tried the new gross tools by Sakura? Or anything else that can cut ONE nickel thick. Tired of reprocessing. Bruce Gapinsk HT (ASCP) Chief Histologist Marin Medical Laboratories PathGroup SF ________________________________ Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Tue Feb 12 12:01:34 2013 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Tue Feb 12 12:01:55 2013 Subject: [Histonet] RE: grossing tools In-Reply-To: References: Message-ID: <761E2B5697F795489C8710BCC72141FF054CC7@ex07.net.ucsf.edu> Bruce, I tried them out at the Sakura demo lab in torrence. They worked really well. Now, whether you can get cranky PA's and residents to use them is another question. Tim -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bruce Gapinski Sent: Tuesday, February 12, 2013 9:39 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] grossing tools Histonians, I'm sure we are not the only histology lab that deals with thick grossed specimens. Has anyone tried the new gross tools by Sakura? Or anything else that can cut ONE nickel thick. Tired of reprocessing. Bruce Gapinsk HT (ASCP) Chief Histologist Marin Medical Laboratories PathGroup SF ________________________________ Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Tue Feb 12 12:12:31 2013 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Tue Feb 12 12:12:41 2013 Subject: [Histonet] RE: Her2neu controls In-Reply-To: References: Message-ID: <14E2C6176416974295479C64A11CB9AE016411AB46AB@SBS2K8.premierlab.local> First of all I want to state that I'm not a clinical lab, I'm a research lab, but we have performed Her2 IHC. An appropriate control block should contain tissue that is processed in your lab. I would try to prepare a block that contained multiple tissue samples (at least two) one 1+ and one 3+. Ideally I would use 3, negative, 1+ and 3+. If you are using just a 3+ as a control how can you be certain that you are able to consistently stain 1+ samples (meaning is your test sensitive enough). I like the use of tissue that is negative too, this helps rule out false positives. We also like to use negative tissue or tissue components during the protocol development process. Cell blocks provided by the vendor are ok in a pinch, but ideally the samples for controls should be processed in your lab. This same principle should also be used during protocol development and validation. You need to look at tissue with varing staining intensities from no staining to 3+ staining. Just my two cents. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 881-0763 cell (303) 682-9060 fax liz@premierlab.com Ship to address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim [Vickroy.Jim@mhsil.com] Sent: Monday, February 11, 2013 3:53 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Her2neu controls When we order the antibody from Ventana they include some control slides with tissues that are 0, 1+, 2+, and 3+. Currently the lab where we have sent our Her2neu's to be done only uses a 3+ control. I realize that a control that had several levels of staining would be ideal but I am trying to find out how other diagnostic labs are handling their controls for Her2neu. Thanks James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TJohnson <@t> gnf.org Tue Feb 12 12:22:14 2013 From: TJohnson <@t> gnf.org (Teri Johnson) Date: Tue Feb 12 12:22:20 2013 Subject: [Histonet] Re: Stain to ID eosinophils specifically Message-ID: <9F3CFEE76E51B64991C7485270890B402E7154BE@EX4.lj.gnf.org> Hi Colleen, In my Vet Tech training we did blood smears stained with Wright stain. The eosinophils were clearly seen and differentially stained from the neutrophils and basophils. Knowing this, I would probably try a Diff-Quick or Giemsa stain, paying close attention to differentiation steps. Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 From TMcNemar <@t> lmhealth.org Tue Feb 12 12:56:40 2013 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Tue Feb 12 12:56:40 2013 Subject: [Histonet] Process and hold? Message-ID: Hello all, I was wondering about processing breast specimens (needle cores) on Fridays. We have asked our radiology department to try to avoid scheduling these breast biopsies on Fridays since we do not work weekends and are concerned about the extended time in formalin. I am thinking that we can run these specimens on a second processor over Friday night and have someone from the clinical lab come up and drain the paraffin. The tissues would then sit in a warm moist retort until Monday morning when they would be embedded and cut. I think the specimens would be fine. Processing would be complete at that point and they would hold in the unopened retort chamber. Our alternative is to have someone come in every Saturday morning just to remove and embed these specimens..... Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org ________________________________ This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From jqb7 <@t> cdc.gov Tue Feb 12 13:02:05 2013 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/OID/NCEZID)) Date: Tue Feb 12 13:02:15 2013 Subject: [Histonet] RE: Process and hold? In-Reply-To: References: Message-ID: Could you have someone from the clinical lab come up, drain the retort and simply remove the cassettes and wrap in foil? Then they could sit at room temp. Jeanine H. Bartlett Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 404-639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Tuesday, February 12, 2013 1:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Process and hold? Hello all, I was wondering about processing breast specimens (needle cores) on Fridays. We have asked our radiology department to try to avoid scheduling these breast biopsies on Fridays since we do not work weekends and are concerned about the extended time in formalin. I am thinking that we can run these specimens on a second processor over Friday night and have someone from the clinical lab come up and drain the paraffin. The tissues would then sit in a warm moist retort until Monday morning when they would be embedded and cut. I think the specimens would be fine. Processing would be complete at that point and they would hold in the unopened retort chamber. Our alternative is to have someone come in every Saturday morning just to remove and embed these specimens..... Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org ________________________________ This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From trathborne <@t> somerset-healthcare.com Tue Feb 12 13:02:43 2013 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Tue Feb 12 13:02:50 2013 Subject: [Histonet] RE: Process and hold? In-Reply-To: References: Message-ID: <3AD061FE740D464FAC7BF6B5CFB75707599CFBE4@smcmail02.somerset-healthcare.com> We have actually had someone come in on Sundays just for this reason. An option for you might be to try this, and have that person put in a full day. He/she could then take off Monday. It has worked well for us. We also are able to get IHC cases done over the weekend so they can be given to the pathologist first thing Monday morning. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Tuesday, February 12, 2013 1:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Process and hold? Hello all, I was wondering about processing breast specimens (needle cores) on Fridays. We have asked our radiology department to try to avoid scheduling these breast biopsies on Fridays since we do not work weekends and are concerned about the extended time in formalin. I am thinking that we can run these specimens on a second processor over Friday night and have someone from the clinical lab come up and drain the paraffin. The tissues would then sit in a warm moist retort until Monday morning when they would be embedded and cut. I think the specimens would be fine. Processing would be complete at that point and they would hold in the unopened retort chamber. Our alternative is to have someone come in every Saturday morning just to remove and embed these specimens..... Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org ________________________________ This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Feb 12 13:07:31 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Feb 12 13:07:37 2013 Subject: [Histonet] Process and hold? In-Reply-To: References: Message-ID: <1360696051.98176.YahooMailNeo@web163106.mail.bf1.yahoo.com> For me that is an awful idea having the processed biopsies sitting in a "warm moist" almost "paradisiacal" environment?as if it were an ideal situation. Make someone to embed the biopsies. This would be the ideal solution, and I think that your patients deserve it. Ren? J. From: Tom McNemar To: "histonet@lists.utsouthwestern.edu" Sent: Tuesday, February 12, 2013 1:56 PM Subject: [Histonet] Process and hold? Hello all, I was wondering about processing breast specimens (needle cores) on Fridays. We have asked our radiology department to try to avoid scheduling these breast biopsies on Fridays since we do not work weekends and are concerned about the extended time in formalin. I am thinking that we can run these specimens on a second processor over Friday night and have someone from the clinical lab come up? and drain the paraffin.? The tissues would then sit in a warm moist retort until Monday morning when they would be embedded and cut.? I think the specimens would be fine.? Processing would be complete at that point and they would hold in the unopened retort chamber. Our alternative is to have someone come in every Saturday morning just to remove and embed these specimens..... Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org ________________________________ This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From robert_schoonhoven <@t> yahoo.com Tue Feb 12 13:12:25 2013 From: robert_schoonhoven <@t> yahoo.com (Robert Schoonhoven) Date: Tue Feb 12 13:12:31 2013 Subject: [Histonet] Process and hold? In-Reply-To: References: Message-ID: <1360696345.27322.YahooMailNeo@web126203.mail.ne1.yahoo.com> All you need to do is have them drain the chamber and place the cassettes on a nonabsorbent surface and allow them to cool to room temp.?? On Monday morning have the techs put the into the cassette storage on your embedding center and they should be ready to embed witin a few minutes.? As the tissues are processed through paraffin they are protected and will not "dry" out. Robert Schoonhoven, HT/HTL (ASCP) ________________________________ From: Tom McNemar To: "histonet@lists.utsouthwestern.edu" Sent: Tuesday, February 12, 2013 1:56 PM Subject: [Histonet] Process and hold? Hello all, I was wondering about processing breast specimens (needle cores) on Fridays. We have asked our radiology department to try to avoid scheduling these breast biopsies on Fridays since we do not work weekends and are concerned about the extended time in formalin. I am thinking that we can run these specimens on a second processor over Friday night and have someone from the clinical lab come up? and drain the paraffin.? The tissues would then sit in a warm moist retort until Monday morning when they would be embedded and cut.? I think the specimens would be fine.? Processing would be complete at that point and they would hold in the unopened retort chamber. Our alternative is to have someone come in every Saturday morning just to remove and embed these specimens..... Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org ________________________________ This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From marktarango <@t> gmail.com Tue Feb 12 13:24:43 2013 From: marktarango <@t> gmail.com (Mark Tarango) Date: Tue Feb 12 13:24:50 2013 Subject: [Histonet] Process and hold? In-Reply-To: <1360696345.27322.YahooMailNeo@web126203.mail.ne1.yahoo.com> References: <1360696345.27322.YahooMailNeo@web126203.mail.ne1.yahoo.com> Message-ID: I second that! On Tue, Feb 12, 2013 at 11:12 AM, Robert Schoonhoven < robert_schoonhoven@yahoo.com> wrote: > All you need to do is have them drain the chamber and place the cassettes > on a nonabsorbent surface and allow them to cool to room temp. On Monday > morning have the techs put the into the cassette storage on your embedding > center and they should be ready to embed witin a few minutes. As the > tissues are processed through paraffin they are protected and will not > "dry" out. > > > Robert Schoonhoven, HT/HTL (ASCP) > > > ________________________________ > From: Tom McNemar > To: "histonet@lists.utsouthwestern.edu" > > Sent: Tuesday, February 12, 2013 1:56 PM > Subject: [Histonet] Process and hold? > > Hello all, > > I was wondering about processing breast specimens (needle cores) on > Fridays. > > We have asked our radiology department to try to avoid scheduling these > breast biopsies on Fridays since we do not work weekends and are concerned > about the extended time in formalin. > > I am thinking that we can run these specimens on a second processor over > Friday night and have someone from the clinical lab come up and drain the > paraffin. The tissues would then sit in a warm moist retort until Monday > morning when they would be embedded and cut. I think the specimens would > be fine. Processing would be complete at that point and they would hold in > the unopened retort chamber. > > Our alternative is to have someone come in every Saturday morning just to > remove and embed these specimens..... > > > > Tom McNemar, HT(ASCP) > Histology Co-ordinator > Licking Memorial Health Systems > (740) 348-4163 > (740) 348-4166 > tmcnemar@lmhealth.org > www.LMHealth.org > www.LMHealth.org> > > ________________________________ > This e-mail, including attachments, is intended for the sole use of the > individual and/or entity to whom it is addressed, and contains information > from Licking Memorial Health Systems which is confidential or privileged. > If you are not the intended recipient, nor authorized to receive for the > intended recipient, be aware that any disclosure, copying, distribution or > use of the contents of this e-mail and attachments is prohibited. If you > have received this in error, please advise the sender by reply e-mail and > delete the message immediately. You may also contact the LMH Process > Improvement Center at 740-348-4641. E-mail transmissions cannot be > guaranteed to be secure or error-free as information could be intercepted, > corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. > The sender therefore does not accept liability for any errors or omissions > in the contents of this message, which arise as a result of e-mail > transmission. Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From doug.porter <@t> caplab.org Tue Feb 12 13:28:48 2013 From: doug.porter <@t> caplab.org (Douglas Porter) Date: Tue Feb 12 13:25:28 2013 Subject: [Histonet] RE: Process and hold? In-Reply-To: <3AD061FE740D464FAC7BF6B5CFB75707599CFBE4@smcmail02.somerset-healthcare.com> References: <3AD061FE740D464FAC7BF6B5CFB75707599CFBE4@smcmail02.somerset-healthcare.com> Message-ID: <001a01ce0957$2ec41450$8c4c3cf0$@caplab.org> Holding the tissue until you come in on Monday may present issues for ER/PR/Her2 staining. Not to mention you would have to have control tissues run under the same conditions. For the sake of the patient/specimen, we come in and embed them and go home. Doesn't take that much time and works out best for all concerned. Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.porter@caplab.org www.caplab.org ? ? The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, copying, forwarding or capture of this communication is strictly prohibited. If you have received this communication in error, please notify me immediately by return e-mail and delete this and all copies. Thank-you. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Tuesday, February 12, 2013 2:03 PM To: 'Tom McNemar'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Process and hold? We have actually had someone come in on Sundays just for this reason. An option for you might be to try this, and have that person put in a full day. He/she could then take off Monday. It has worked well for us. We also are able to get IHC cases done over the weekend so they can be given to the pathologist first thing Monday morning. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Tuesday, February 12, 2013 1:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Process and hold? Hello all, I was wondering about processing breast specimens (needle cores) on Fridays. We have asked our radiology department to try to avoid scheduling these breast biopsies on Fridays since we do not work weekends and are concerned about the extended time in formalin. I am thinking that we can run these specimens on a second processor over Friday night and have someone from the clinical lab come up and drain the paraffin. The tissues would then sit in a warm moist retort until Monday morning when they would be embedded and cut. I think the specimens would be fine. Processing would be complete at that point and they would hold in the unopened retort chamber. Our alternative is to have someone come in every Saturday morning just to remove and embed these specimens..... Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org ________________________________ This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MSHERWOOD <@t> PARTNERS.ORG Tue Feb 12 13:32:28 2013 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret) Date: Tue Feb 12 13:32:34 2013 Subject: [Histonet] Process and hold? In-Reply-To: References: <1360696345.27322.YahooMailNeo@web126203.mail.ne1.yahoo.com> Message-ID: <16F356143B1CE2459BC129BF68AD0F0F03F9DBBB@PHSX10MB25.partners.org> I also agree. We've done this when the investigator requests being there to help orient tissue. We just remove the cassette and let drain on paper towel. Have never had a problem. (I wouldn't leave in liquid, hot paraffin, though). Peggy Sherwood Research Specialist, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Tarango Sent: Tuesday, February 12, 2013 2:25 PM To: Robert Schoonhoven Cc: histonet@lists.utsouthwestern.edu; Tom McNemar Subject: Re: [Histonet] Process and hold? I second that! On Tue, Feb 12, 2013 at 11:12 AM, Robert Schoonhoven < robert_schoonhoven@yahoo.com> wrote: > All you need to do is have them drain the chamber and place the cassettes > on a nonabsorbent surface and allow them to cool to room temp. On Monday > morning have the techs put the into the cassette storage on your embedding > center and they should be ready to embed witin a few minutes. As the > tissues are processed through paraffin they are protected and will not > "dry" out. > > > Robert Schoonhoven, HT/HTL (ASCP) > > > ________________________________ > From: Tom McNemar > To: "histonet@lists.utsouthwestern.edu" > > Sent: Tuesday, February 12, 2013 1:56 PM > Subject: [Histonet] Process and hold? > > Hello all, > > I was wondering about processing breast specimens (needle cores) on > Fridays. > > We have asked our radiology department to try to avoid scheduling these > breast biopsies on Fridays since we do not work weekends and are concerned > about the extended time in formalin. > > I am thinking that we can run these specimens on a second processor over > Friday night and have someone from the clinical lab come up and drain the > paraffin. The tissues would then sit in a warm moist retort until Monday > morning when they would be embedded and cut. I think the specimens would > be fine. Processing would be complete at that point and they would hold in > the unopened retort chamber. > > Our alternative is to have someone come in every Saturday morning just to > remove and embed these specimens..... > > > > Tom McNemar, HT(ASCP) > Histology Co-ordinator > Licking Memorial Health Systems > (740) 348-4163 > (740) 348-4166 > tmcnemar@lmhealth.org > www.LMHealth.org > www.LMHealth.org> > > ________________________________ > This e-mail, including attachments, is intended for the sole use of the > individual and/or entity to whom it is addressed, and contains information > from Licking Memorial Health Systems which is confidential or privileged. > If you are not the intended recipient, nor authorized to receive for the > intended recipient, be aware that any disclosure, copying, distribution or > use of the contents of this e-mail and attachments is prohibited. If you > have received this in error, please advise the sender by reply e-mail and > delete the message immediately. You may also contact the LMH Process > Improvement Center at 740-348-4641. E-mail transmissions cannot be > guaranteed to be secure or error-free as information could be intercepted, > corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. > The sender therefore does not accept liability for any errors or omissions > in the contents of this message, which arise as a result of e-mail > transmission. Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From lcolbert <@t> pathmdlabs.com Tue Feb 12 14:01:55 2013 From: lcolbert <@t> pathmdlabs.com (Laurie Colbert) Date: Tue Feb 12 14:08:26 2013 Subject: [Histonet] STP420 Tissue Processor Message-ID: <12ECD7346266D74691EC2BFC75285E450119B227@BFL323E10.pathmdlabs.local> Is there anyone out there who processes both large and small tissue on the Thermo STP420 tissue processor using both the rotation chamber and the biopsy chamber? Would you please email me directly? Thanks. Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab From Joyce.Weems <@t> emoryhealthcare.org Tue Feb 12 14:33:32 2013 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Tue Feb 12 14:33:43 2013 Subject: [Histonet] RE: Process and hold? In-Reply-To: References: Message-ID: We have someone from the clinical lab drain the processor, but we have them remove the tissue and just leave it out - that way it doesn't cook.. we've had no problems with this for a couple of years, when we finally got our pathologists talked into letting us stop working Saturdays. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Tuesday, February 12, 2013 1:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Process and hold? Hello all, I was wondering about processing breast specimens (needle cores) on Fridays. We have asked our radiology department to try to avoid scheduling these breast biopsies on Fridays since we do not work weekends and are concerned about the extended time in formalin. I am thinking that we can run these specimens on a second processor over Friday night and have someone from the clinical lab come up and drain the paraffin. The tissues would then sit in a warm moist retort until Monday morning when they would be embedded and cut. I think the specimens would be fine. Processing would be complete at that point and they would hold in the unopened retort chamber. Our alternative is to have someone come in every Saturday morning just to remove and embed these specimens..... Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org ________________________________ This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From amber.mckenzie <@t> gastrodocs.net Tue Feb 12 15:18:04 2013 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Tue Feb 12 15:18:12 2013 Subject: [Histonet] Senority Question In-Reply-To: References: Message-ID: <5A33C952BB67F4468AF1F36D739212BCBCEACFEE@JERRY.Gia.com> I have 2 employees that are wondering about seniority... one HT has been on payroll for 5 years and has gone from FT to PRN and back to FT without any breaks. The other tech has been FT for 3 years. Which one is the senior in the lab? From rjbuesa <@t> yahoo.com Tue Feb 12 15:48:55 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Feb 12 15:48:59 2013 Subject: [Histonet] Senority Question In-Reply-To: <5A33C952BB67F4468AF1F36D739212BCBCEACFEE@JERRY.Gia.com> References: <5A33C952BB67F4468AF1F36D739212BCBCEACFEE@JERRY.Gia.com> Message-ID: <1360705735.6968.YahooMailNeo@web163103.mail.bf1.yahoo.com> For me it is the first one because (for me at least) it is not for how long the employee has been FT but for how long an employee has been involved with the lab and being able, with his/her performance gained the trust of the employer. Ren? J. From: Amber McKenzie To: "histonet@lists.utsouthwestern.edu" Sent: Tuesday, February 12, 2013 4:18 PM Subject: [Histonet] Senority Question I have 2 employees that are wondering about seniority... one HT has been on payroll for 5 years and has gone from FT to PRN and back to FT without any breaks.? The other tech has been FT for 3 years. Which one is the senior in the lab? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amosbrooks <@t> gmail.com Tue Feb 12 16:34:41 2013 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Tue Feb 12 16:34:48 2013 Subject: [Histonet] Stain to ID eosinophils specifically Message-ID: Hi Colleen, Although I was not able to locate the specific reference you listed, the two stains that come to mind are Lendrum's Carbol Chromotrope and Congo Red. While they are not *really* specific for eosinophils, they do stain eosinophils well. It is fairly easy to differentiate an eosinophil from amyloid in Congo Red. I am including a link to Eastwood & Cole's Congo Red because the procedure specifically mentions eosinophils, but I have used Highman's (the more common version) to identify them. Of the two the Congo Red techniques seem to work better which is great since they are a bit easier (& less messy) than the carbol chromotrope. Here are a couple of references for both: http://stainsfile.info/StainsFile/stain/cell/lendrum-carbolchromotrope.htm and http://stainsfile.info/StainsFile/stain/amyloid/congoeastwood.htm Good Luck, Amos On Tue, Feb 12, 2013 at 1:00 PM, wrote: > Message: 6 > Date: Mon, 11 Feb 2013 16:36:29 -0600 > From: Colleen Forster > Subject: [Histonet] Stain to ID eosinophils specifically > To: Histonet > Message-ID: <5119726D.7000509@umn.edu> > Content-Type: text/plain; charset="iso-8859-1" > > Does anyone have access to this reference for me? I have a person looking > to ID eosinophils in fat samples. > I am looking for a stain that will make finding them easier....this > reference was mentioned....any other suggestions? These are FFPE samples. > > Suggested Ref ex. LIllie 4th Ed. pp750. ref.Am.J.Clin.Path.55:283,1971.) > > Thanks in advance... > > Colleen L. Forster > From rsrichmond <@t> gmail.com Tue Feb 12 19:01:51 2013 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Tue Feb 12 19:03:04 2013 Subject: [Histonet] Re: grossing tools Message-ID: Bruce Gapinsk HT (ASCP), Chief Histologist, Marin Medical Laboratories, PathGroup SF asks: >>Histonians, I'm sure we are not the only histology lab that deals with thick grossed specimens. Has anyone tried the new gross tools by Sakura? Or anything else that can cut ONE nickel thick. Tired of reprocessing.<< This grumpy old (74) pathologist would be happy to try them out, though I can cut freehand and almost never have a block reprocessed, but the skill eludes a lot of the young folks. Do you have a Web link for these tools? The problem would be getting a lab manager to spring for them. The Laboratory Manager's Handy-Dandy Guide to Making Life Hard for the Pathologist (I'm sure there is such a book, though I haven't actually seen one) specifies that grossing is a ritual requiring only a basic set of tools. One lab expected me to gross with an athame and chalice. I tried to explain to them that OSHA and the CAP would not permit me to gross skyclad. (You guys know I NEVER exaggerate.) Bob Richmond Samurai Pathologist Maryville TN From joelleweaver <@t> hotmail.com Wed Feb 13 00:00:31 2013 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Wed Feb 13 00:00:38 2013 Subject: [Histonet] Re: grossing tools In-Reply-To: References: Message-ID: Completely hilarious! Joelle Weaver MAOM, HTL (ASCP) QIHC > Date: Tue, 12 Feb 2013 20:01:51 -0500 > From: rsrichmond@gmail.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: grossing tools > > Bruce Gapinsk HT (ASCP), Chief Histologist, Marin Medical > Laboratories, PathGroup SF asks: > > >>Histonians, I'm sure we are not the only histology lab that deals with thick grossed specimens. Has anyone tried the new gross tools by Sakura? Or anything else that can cut ONE nickel thick. Tired of reprocessing.<< > > This grumpy old (74) pathologist would be happy to try them out, > though I can cut freehand and almost never have a block reprocessed, > but the skill eludes a lot of the young folks. Do you have a Web link > for these tools? > > The problem would be getting a lab manager to spring for them. The > Laboratory Manager's Handy-Dandy Guide to Making Life Hard for the > Pathologist (I'm sure there is such a book, though I haven't actually > seen one) specifies that grossing is a ritual requiring only a basic > set of tools. > > One lab expected me to gross with an athame and chalice. I tried to > explain to them that OSHA and the CAP would not permit me to gross > skyclad. > > (You guys know I NEVER exaggerate.) > > Bob Richmond > Samurai Pathologist > Maryville TN > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Susan.Walzer <@t> HCAHealthcare.com Wed Feb 13 02:14:42 2013 From: Susan.Walzer <@t> HCAHealthcare.com (Susan.Walzer@HCAHealthcare.com) Date: Wed Feb 13 02:14:51 2013 Subject: [Histonet] RE: Sonority Question In-Reply-To: <5A33C952BB67F4468AF1F36D739212BCBCEACFEE@JERRY.Gia.com> References: <5A33C952BB67F4468AF1F36D739212BCBCEACFEE@JERRY.Gia.com> Message-ID: <4BF03F5404EBDE409AF9232DA74B9DED2DFD4DFBB6@FWDCWPMSGCMS09.hca.corpad.net> I would have to consider the tech who is working continuously as senior. That is the tech that I would rely on the most. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Tuesday, February 12, 2013 4:18 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Senority Question I have 2 employees that are wondering about seniority... one HT has been on payroll for 5 years and has gone from FT to PRN and back to FT without any breaks. The other tech has been FT for 3 years. Which one is the senior in the lab? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From d-emge <@t> northwestern.edu Wed Feb 13 06:57:56 2013 From: d-emge <@t> northwestern.edu (Donna J Emge) Date: Wed Feb 13 06:58:01 2013 Subject: [Histonet] RE: Process and Hold? Message-ID: <54C3042A8564BA4AA2D355DD1D4C949C11ABC7A4@CHCSPMBX1.ads.northwestern.edu> Tom, another idea is to set your processor to do the weekend delay hold in 70% alcohol rather than in the formalin. Then the samples will go through the formalin as usual, and stop and hold in the 70% until weekend processing as scheduled begins. Then your samples will come off on Monday morning as usual, without extra fixation, and without requiring a weekend technician. The 70% alcohol does no harm at all to your samples. We do this routinely with other tissues for IHC in our lab with great results. Donna Donna J. Emge, HT-ASCP Mouse Histology and Phenotyping Core Manager Northwestern University Olson Building room 8-333 710 North Fairbanks Court Chicago, IL 60611 MHPL@northwestern.edu 312-503-2679 From mturner <@t> CSILaboratories.com Wed Feb 13 07:01:39 2013 From: mturner <@t> CSILaboratories.com (Mark Turner) Date: Wed Feb 13 07:02:26 2013 Subject: [Histonet] RE: Sonority Question In-Reply-To: <4BF03F5404EBDE409AF9232DA74B9DED2DFD4DFBB6@FWDCWPMSGCMS09.hca.corpad.net> References: <5A33C952BB67F4468AF1F36D739212BCBCEACFEE@JERRY.Gia.com> <4BF03F5404EBDE409AF9232DA74B9DED2DFD4DFBB6@FWDCWPMSGCMS09.hca.corpad.net> Message-ID: <643626B74DE2814D8537057F40E1A10B03CA5BC2@CSI-MX-NODEA.CSI-LABS.local> I have worked in places which do both. One lab considered hire date only while another calculated FT equivalent hours. Both have merit and it is usually set according to institutional policy. Mark Turner, Ph.D., HT(ASCP)QIHC Manager, Histology/IHC CSI Laboratories ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Susan.Walzer@HCAHealthcare.com Sent: Wednesday, February 13, 2013 3:15 AM To: amber.mckenzie@gastrodocs.net; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Sonority Question I would have to consider the tech who is working continuously as senior. That is the tech that I would rely on the most. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Tuesday, February 12, 2013 4:18 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Senority Question I have 2 employees that are wondering about seniority... one HT has been on payroll for 5 years and has gone from FT to PRN and back to FT without any breaks. The other tech has been FT for 3 years. Which one is the senior in the lab? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mikael.niku <@t> helsinki.fi Wed Feb 13 07:15:57 2013 From: mikael.niku <@t> helsinki.fi (Mikael Niku) Date: Wed Feb 13 07:16:46 2013 Subject: [Histonet] Tissue processing for laser microdissection and RNA isolation? Message-ID: <511B920D.9030903@helsinki.fi> Hello! May I ask for your recommendations for tissue processing methods for laser microdissection and subsequent RNA isolation? I can think of at least the following protocols, each with significant drawbacks (and questions): 1) Traditional FFPE sections: + easy handling + RNA is safe (but see below) + good morphology - RNA is fixed too, so yields are low and only small fragments retrieved Here, I'm pretty happy with Qiagen RNEasy FFPE kit - any other suggestions, maybe cheaper? 2) Traditional cryosections: + fairly good morphology + good yields, good quality RNA if everything goes well - RNA is easily destroyed - difficult to handle small samples without melting & destroying RNA I haven't been very succesful with this option. 3) RNALater -> cryosections: + RNA is safe + good RNA yields, good quality RNA - poor morphology - difficult to section We have problems making the tissues actually freeze for good sectioning - any tricks or tips here? 4) RNALater -> paraffin sections? I haven't tried this yet, but should be doable through ethanol etc. Found some references claiming that the RNA quality is poor. 5) New commercial innovations like Qiagen/Prenalytix Paxgene Tissue kit, claiming to achieve both RNA stabilization and good morphology. I haven't tried any of these yet. Pricing is the obvious drawback. With best regards, Mikael Niku, PhD Department of Veterinary Biosciences University of Helsinki From sccrshlly <@t> yahoo.com Wed Feb 13 07:50:57 2013 From: sccrshlly <@t> yahoo.com (Michelle Coker) Date: Wed Feb 13 07:51:00 2013 Subject: [Histonet] Process and hold? In-Reply-To: <1360696345.27322.YahooMailNeo@web126203.mail.ne1.yahoo.com> References: <1360696345.27322.YahooMailNeo@web126203.mail.ne1.yahoo.com> Message-ID: <51EABD6F-E64E-44E7-88C1-64B22BB4BB60@yahoo.com> We do this on a fairly routine basis with our GI biopsies and have found it to be most effective. Leaving them in the warm retort exposes the tissue to heat longer than normal and could cause some problems. On Feb 12, 2013, at 1:12 PM, Robert Schoonhoven wrote: > All you need to do is have them drain the chamber and place the cassettes on a nonabsorbent surface and allow them to cool to room temp. On Monday morning have the techs put the into the cassette storage on your embedding center and they should be ready to embed witin a few minutes. As the tissues are processed through paraffin they are protected and will not "dry" out. > > > Robert Schoonhoven, HT/HTL (ASCP) > > > ________________________________ > From: Tom McNemar > To: "histonet@lists.utsouthwestern.edu" > Sent: Tuesday, February 12, 2013 1:56 PM > Subject: [Histonet] Process and hold? > > Hello all, > > I was wondering about processing breast specimens (needle cores) on Fridays. > > We have asked our radiology department to try to avoid scheduling these breast biopsies on Fridays since we do not work weekends and are concerned about the extended time in formalin. > > I am thinking that we can run these specimens on a second processor over Friday night and have someone from the clinical lab come up and drain the paraffin. The tissues would then sit in a warm moist retort until Monday morning when they would be embedded and cut. I think the specimens would be fine. Processing would be complete at that point and they would hold in the unopened retort chamber. > > Our alternative is to have someone come in every Saturday morning just to remove and embed these specimens..... > > > > Tom McNemar, HT(ASCP) > Histology Co-ordinator > Licking Memorial Health Systems > (740) 348-4163 > (740) 348-4166 > tmcnemar@lmhealth.org > www.LMHealth.org > > ________________________________ > This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > /lists.utsouthwestern.edu/mailman/listinfo/histonet From MMargiotta <@t> bmhmc.org Wed Feb 13 08:24:39 2013 From: MMargiotta <@t> bmhmc.org (Margiotta-Watz, Michele) Date: Wed Feb 13 08:24:45 2013 Subject: [Histonet] RE: Process and Hold? In-Reply-To: <54C3042A8564BA4AA2D355DD1D4C949C11ABC7A4@CHCSPMBX1.ads.northwestern.edu> References: <54C3042A8564BA4AA2D355DD1D4C949C11ABC7A4@CHCSPMBX1.ads.northwestern.edu> Message-ID: <230D0B9EC57D7A45A7A186C6AB4C7ABC296E3892@BMH-EXCHANGE-01.BMHMC.ORG> We do the same thing with good results! Michele -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Donna J Emge Sent: Wednesday, February 13, 2013 7:58 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Process and Hold? Tom, another idea is to set your processor to do the weekend delay hold in 70% alcohol rather than in the formalin. Then the samples will go through the formalin as usual, and stop and hold in the 70% until weekend processing as scheduled begins. Then your samples will come off on Monday morning as usual, without extra fixation, and without requiring a weekend technician. The 70% alcohol does no harm at all to your samples. We do this routinely with other tissues for IHC in our lab with great results. Donna Donna J. Emge, HT-ASCP Mouse Histology and Phenotyping Core Manager Northwestern University Olson Building room 8-333 710 North Fairbanks Court Chicago, IL 60611 MHPL@northwestern.edu 312-503-2679 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet DISCLAIMER: This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. From billodonnell <@t> catholichealth.net Wed Feb 13 08:39:46 2013 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Wed Feb 13 08:39:47 2013 Subject: [Histonet] Re: grossing tools In-Reply-To: References: Message-ID: <4940DF6D1C5FDF48931B6966AAEF9395A1A224@chimsx08.CHI.catholichealth.net> The book exists - I've seen it. I think it was written by some guy who gives management seminars. But it mysteriously vanished - probably taken by aliens to de-evolutionize their home planet. That's my theory. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Tuesday, February 12, 2013 7:02 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: grossing tools Bruce Gapinsk HT (ASCP), Chief Histologist, Marin Medical Laboratories, PathGroup SF asks: >>Histonians, I'm sure we are not the only histology lab that deals with >>thick grossed specimens. Has anyone tried the new gross tools by >>Sakura? Or anything else that can cut ONE nickel thick. Tired of >>reprocessing.<< This grumpy old (74) pathologist would be happy to try them out, though I can cut freehand and almost never have a block reprocessed, but the skill eludes a lot of the young folks. Do you have a Web link for these tools? The problem would be getting a lab manager to spring for them. The Laboratory Manager's Handy-Dandy Guide to Making Life Hard for the Pathologist (I'm sure there is such a book, though I haven't actually seen one) specifies that grossing is a ritual requiring only a basic set of tools. One lab expected me to gross with an athame and chalice. I tried to explain to them that OSHA and the CAP would not permit me to gross skyclad. (You guys know I NEVER exaggerate.) Bob Richmond Samurai Pathologist Maryville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. From linda555m <@t> yahoo.com Wed Feb 13 08:42:46 2013 From: linda555m <@t> yahoo.com (Linda Musto) Date: Wed Feb 13 08:42:50 2013 Subject: [Histonet] H.Pylori Control Blocks Message-ID: <1360766566.17159.YahooMailNeo@web120406.mail.ne1.yahoo.com> Good Morning, ?We are in need of H.Pylori controls. Does anyone one know where?we can get?FFPE blocks positive for H.pylori?? ??????????????????????????? Thank you, ?????????????????????????????? Linda Musto From rsrichmond <@t> gmail.com Wed Feb 13 08:53:53 2013 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Wed Feb 13 08:53:58 2013 Subject: [Histonet] Re: grossing tools In-Reply-To: References: Message-ID: Jeanine H. Bartlett at the CDC notes: >>Sakura sells these. The grossing boards are items: 4800, 4801 and 4802. The grossing forks are 4803, 4804 and 4807. Ask your rep for a demo. http://sakura.ifuture.com/sakura.asp?ifut=1000336642970309899%2CTKWK%3E6%3D%3A5ST0 The prices of these tools are outrageous. The online catalog gives me no clue as to how they work. Bob Richmond Samurai Pathologist Maryville TN ************************************************ On Tue, Feb 12, 2013 at 8:01 PM, Bob Richmond wrote: > Bruce Gapinsk HT (ASCP), Chief Histologist, Marin Medical > Laboratories, PathGroup SF asks: > >>>Histonians, I'm sure we are not the only histology lab that deals with thick grossed specimens. Has anyone tried the new gross tools by Sakura? Or anything else that can cut ONE nickel thick. Tired of reprocessing.<< > > This grumpy old (74) pathologist would be happy to try them out, > though I can cut freehand and almost never have a block reprocessed, > but the skill eludes a lot of the young folks. Do you have a Web link > for these tools? > > The problem would be getting a lab manager to spring for them. The > Laboratory Manager's Handy-Dandy Guide to Making Life Hard for the > Pathologist (I'm sure there is such a book, though I haven't actually > seen one) specifies that grossing is a ritual requiring only a basic > set of tools. > > One lab expected me to gross with an athame and chalice. I tried to > explain to them that OSHA and the CAP would not permit me to gross > skyclad. > > (You guys know I NEVER exaggerate.) > > Bob Richmond > Samurai Pathologist > Maryville TN From jqb7 <@t> cdc.gov Wed Feb 13 08:57:02 2013 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/OID/NCEZID)) Date: Wed Feb 13 08:57:19 2013 Subject: [Histonet] Re: grossing tools In-Reply-To: References: Message-ID: That is why I suggested having a rep. bring them out to demonstrate. "Sakura sells these. The grossing boards are items: 4800, 4801 and 4802. The grossing forks are 4803, 4804 and 4807. Ask your rep for a demo." Jeanine H. Bartlett Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 404-639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Wednesday, February 13, 2013 9:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: grossing tools Jeanine H. Bartlett at the CDC notes: >>Sakura sells these. The grossing boards are items: 4800, 4801 and 4802. The grossing forks are 4803, 4804 and 4807. Ask your rep for a demo. http://sakura.ifuture.com/sakura.asp?ifut=1000336642970309899%2CTKWK%3E6%3D%3A5ST0 The prices of these tools are outrageous. The online catalog gives me no clue as to how they work. Bob Richmond Samurai Pathologist Maryville TN ************************************************ On Tue, Feb 12, 2013 at 8:01 PM, Bob Richmond wrote: > Bruce Gapinsk HT (ASCP), Chief Histologist, Marin Medical > Laboratories, PathGroup SF asks: > >>>Histonians, I'm sure we are not the only histology lab that deals >>>with thick grossed specimens. Has anyone tried the new gross tools by >>>Sakura? Or anything else that can cut ONE nickel thick. Tired of >>>reprocessing.<< > > This grumpy old (74) pathologist would be happy to try them out, > though I can cut freehand and almost never have a block reprocessed, > but the skill eludes a lot of the young folks. Do you have a Web link > for these tools? > > The problem would be getting a lab manager to spring for them. The > Laboratory Manager's Handy-Dandy Guide to Making Life Hard for the > Pathologist (I'm sure there is such a book, though I haven't actually > seen one) specifies that grossing is a ritual requiring only a basic > set of tools. > > One lab expected me to gross with an athame and chalice. I tried to > explain to them that OSHA and the CAP would not permit me to gross > skyclad. > > (You guys know I NEVER exaggerate.) > > Bob Richmond > Samurai Pathologist > Maryville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bill501 <@t> mindspring.com Wed Feb 13 08:57:54 2013 From: bill501 <@t> mindspring.com (Bill B.) Date: Wed Feb 13 08:58:18 2013 Subject: [Histonet] grossing tools In-Reply-To: References: Message-ID: At 5:38 PM +0000 2/12/13, Bruce Gapinski wrote: > I'm sure we are not the only histology lab that deals with thick grossed specimens. Has anyone tried the new gross tools by Sakura? Or anything else that can cut ONE nickel thick.<< A bane indeed, not only for histotechs, but for those doing the grossing. Our big problem is breast biopsies. The combination of fat and fibrous tissue makes it hard (impossible) to get consistent thicknesses manually. I have been trying to find some kind 'jig' that would hold inked breast biopsies of widely varying sizes that could guide the knife as the biopsy is bread-loafed to get consistent thicknesses. I'm not sure what words to look for or search for. OTOH, given our difficulty in getting paid for the tech component (we are an independent lab, long used to global billing), I dont want to spend a fortune for something. All advice is super-greatly appreciated. Bill -- ______________ Bill Blank, MD Heartland Lab From TMcNemar <@t> lmhealth.org Wed Feb 13 09:18:12 2013 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Wed Feb 13 09:18:11 2013 Subject: [Histonet] Re: grossing tools In-Reply-To: References: Message-ID: We have a pair of the 3mm Cutmate forceps that we bought years ago for a particular pathologist but nobody uses them. If you go to the website below check out the Procut as well. http://www.milestonemed.com/histopathology/products.html Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/OID/NCEZID) Sent: Wednesday, February 13, 2013 9:57 AM To: Bob Richmond; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: grossing tools That is why I suggested having a rep. bring them out to demonstrate. "Sakura sells these. The grossing boards are items: 4800, 4801 and 4802. The grossing forks are 4803, 4804 and 4807. Ask your rep for a demo." Jeanine H. Bartlett Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 404-639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Wednesday, February 13, 2013 9:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: grossing tools Jeanine H. Bartlett at the CDC notes: >>Sakura sells these. The grossing boards are items: 4800, 4801 and 4802. The grossing forks are 4803, 4804 and 4807. Ask your rep for a demo. http://sakura.ifuture.com/sakura.asp?ifut=1000336642970309899%2CTKWK%3E6%3D%3A5ST0 The prices of these tools are outrageous. The online catalog gives me no clue as to how they work. Bob Richmond Samurai Pathologist Maryville TN ************************************************ On Tue, Feb 12, 2013 at 8:01 PM, Bob Richmond wrote: > Bruce Gapinsk HT (ASCP), Chief Histologist, Marin Medical > Laboratories, PathGroup SF asks: > >>>Histonians, I'm sure we are not the only histology lab that deals >>>with thick grossed specimens. Has anyone tried the new gross tools by >>>Sakura? Or anything else that can cut ONE nickel thick. Tired of >>>reprocessing.<< > > This grumpy old (74) pathologist would be happy to try them out, > though I can cut freehand and almost never have a block reprocessed, > but the skill eludes a lot of the young folks. Do you have a Web link > for these tools? > > The problem would be getting a lab manager to spring for them. The > Laboratory Manager's Handy-Dandy Guide to Making Life Hard for the > Pathologist (I'm sure there is such a book, though I haven't actually > seen one) specifies that grossing is a ritual requiring only a basic > set of tools. > > One lab expected me to gross with an athame and chalice. I tried to > explain to them that OSHA and the CAP would not permit me to gross > skyclad. > > (You guys know I NEVER exaggerate.) > > Bob Richmond > Samurai Pathologist > Maryville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From rjbuesa <@t> yahoo.com Wed Feb 13 09:20:53 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Feb 13 09:21:02 2013 Subject: [Histonet] Tissue processing for laser microdissection and RNA isolation? In-Reply-To: <511B920D.9030903@helsinki.fi> References: <511B920D.9030903@helsinki.fi> Message-ID: <1360768853.77001.YahooMailNeo@web163106.mail.bf1.yahoo.com> If you fix adequately (no NBF) you can go with 1 Ren? J. From: Mikael Niku To: histonet@lists.utsouthwestern.edu Sent: Wednesday, February 13, 2013 8:15 AM Subject: [Histonet] Tissue processing for laser microdissection and RNA isolation? Hello! May I ask for your recommendations for tissue processing methods for laser microdissection and subsequent RNA isolation? I can think of at least the following protocols, each with significant drawbacks (and questions): 1) Traditional FFPE sections: + easy handling + RNA is safe (but see below) + good morphology - RNA is fixed too, so yields are low and only small fragments retrieved Here, I'm pretty happy with Qiagen RNEasy FFPE kit - any other suggestions, maybe cheaper? 2) Traditional cryosections: + fairly good morphology + good yields, good quality RNA if everything goes well - RNA is easily destroyed - difficult to handle small samples without melting & destroying RNA I haven't been very succesful with this option. 3) RNALater -> cryosections: + RNA is safe + good RNA yields, good quality RNA - poor morphology - difficult to section We have problems making the tissues actually freeze for good sectioning - any tricks or tips here? 4) RNALater -> paraffin sections? I haven't tried this yet, but should be doable through ethanol etc. Found some references claiming that the RNA quality is poor. 5) New commercial innovations like Qiagen/Prenalytix Paxgene Tissue kit, claiming to achieve both RNA stabilization and good morphology. I haven't tried any of these yet. Pricing is the obvious drawback. With best regards, Mikael Niku, PhD Department of Veterinary Biosciences University of Helsinki _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Wed Feb 13 09:31:09 2013 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Wed Feb 13 09:31:23 2013 Subject: [Histonet] Process and hold? In-Reply-To: References: Message-ID: <511B6B6C.7770.0077.1@harthosp.org> I'm surprised that laboratories are not validating extended fixation times for their breast specimens rather than "jumping through all these hoops". There are now several published articles demonstrating no reduction in immunoreactivity for ER, PR, and HER2 in breast specimens kept in formalin for 72 hours or longer (one of which is listed below). Please remember that the "total time in formalin" is only one of the pre-analytic variables that can affect immunoreactivity. Minimizing the cold ischemic time, making sure that the fresh tissue does not dry-out, and submitting "THIN" (2-3 mm) tissue sections for fixation and processing are equally, if not more important, than the total time that a specimen sits in formalin. Tong LC, Nelson N, Tsourigiannis J, et al.: The effect of prolonged fixation on the IHC evaluation of ER, PR, and HER2 expression in invasive breast cancer: A prospective study. Am J Surg Pathol 2011;35:545-552. A summation of their study; "... fixation for limited periods beyond 72 hours does not result in a reduction in assay sensitivity in the determination of ER, PR, or HER2 IHC status.". Obviously, each laboratory must do their own testing and validation, but it can be accomplished with team work (Pathologists, PAs, and Histotechnologists working together). Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-2204 Fax >>> Tom McNemar 2/12/2013 1:56 PM >>> Hello all, I was wondering about processing breast specimens (needle cores) on Fridays. We have asked our radiology department to try to avoid scheduling these breast biopsies on Fridays since we do not work weekends and are concerned about the extended time in formalin. I am thinking that we can run these specimens on a second processor over Friday night and have someone from the clinical lab come up and drain the paraffin. The tissues would then sit in a warm moist retort until Monday morning when they would be embedded and cut. I think the specimens would be fine. Processing would be complete at that point and they would hold in the unopened retort chamber. Our alternative is to have someone come in every Saturday morning just to remove and embed these specimens..... Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org ________________________________ This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Wed Feb 13 09:54:01 2013 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Wed Feb 13 09:54:15 2013 Subject: [Histonet] RE: Sonority Question In-Reply-To: <643626B74DE2814D8537057F40E1A10B03CA5BC2@CSI-MX-NODEA.CSI-LABS.local> References: <5A33C952BB67F4468AF1F36D739212BCBCEACFEE@JERRY.Gia.com> <4BF03F5404EBDE409AF9232DA74B9DED2DFD4DFBB6@FWDCWPMSGCMS09.hca.corpad.net> <643626B74DE2814D8537057F40E1A10B03CA5BC2@CSI-MX-NODEA.CSI-LABS.local> Message-ID: <761E2B5697F795489C8710BCC72141FF055050@ex07.net.ucsf.edu> I wouldn't even touch that question and instead refer it to the all-knowing HR department. Too many legal issues involved in that one...and if there is a union involved they will have a say in it. Tim Morken UCSF Pathology -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Tuesday, February 12, 2013 4:18 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Senority Question I have 2 employees that are wondering about seniority... one HT has been on payroll for 5 years and has gone from FT to PRN and back to FT without any breaks. The other tech has been FT for 3 years. Which one is the senior in the lab? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Joyce.Weems <@t> emoryhealthcare.org Wed Feb 13 10:09:22 2013 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Wed Feb 13 10:09:29 2013 Subject: [Histonet] grossing tools In-Reply-To: References: Message-ID: I have found squeezing the tissue (for the block sections) between two cassettes held back to back gives the firmness needed to trim good thin sections. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bill B. Sent: Wednesday, February 13, 2013 9:58 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] grossing tools At 5:38 PM +0000 2/12/13, Bruce Gapinski wrote: > I'm sure we are not the only histology lab that deals with thick grossed specimens. Has anyone tried the new gross tools by Sakura? Or anything else that can cut ONE nickel thick.<< A bane indeed, not only for histotechs, but for those doing the grossing. Our big problem is breast biopsies. The combination of fat and fibrous tissue makes it hard (impossible) to get consistent thicknesses manually. I have been trying to find some kind 'jig' that would hold inked breast biopsies of widely varying sizes that could guide the knife as the biopsy is bread-loafed to get consistent thicknesses. I'm not sure what words to look for or search for. OTOH, given our difficulty in getting paid for the tech component (we are an independent lab, long used to global billing), I dont want to spend a fortune for something. All advice is super-greatly appreciated. Bill -- ______________ Bill Blank, MD Heartland Lab _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From ncosenza <@t> siumed.edu Wed Feb 13 10:37:44 2013 From: ncosenza <@t> siumed.edu (Nicole Cosenza) Date: Wed Feb 13 10:38:29 2013 Subject: [Histonet] nerve embedding/staining Message-ID: <511BC158.1020108@siumed.edu> My lab is thinking of performing a nerve study. We want to IF stain cross sections of nerve for a few different antigens of interest. The nerve will only be 5mm in length. Is it better to paraffin embed and section or cryosection? Is there a way to stain the tissue to it can be seen in the block WITHOUT affecting later IF staining? From rjbuesa <@t> yahoo.com Wed Feb 13 11:05:02 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Feb 13 11:05:11 2013 Subject: [Histonet] grossing tools In-Reply-To: References: Message-ID: <1360775102.89849.YahooMailNeo@web163105.mail.bf1.yahoo.com> How much squeezing? Tissues have certain elasticity and after the squeezing they will "bounce back" as a thicker slice. "Free hand" sectioning I think is always better. Ren? J. From: "Weems, Joyce K." To: 'Bill B.' ; "histonet@lists.utsouthwestern.edu" Sent: Wednesday, February 13, 2013 11:09 AM Subject: RE: [Histonet] grossing tools I have found squeezing the tissue (for the block sections) between two cassettes held back to back gives the firmness needed to trim good thin sections. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org http://www.saintjosephsatlanta.org/ 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s).? It may contain information that is privileged and confidential.? Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bill B. Sent: Wednesday, February 13, 2013 9:58 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] grossing tools At 5:38 PM +0000 2/12/13, Bruce Gapinski wrote: >? ? I'm sure we are not the only histology lab that deals with thick grossed specimens. Has anyone tried the new gross tools by Sakura? Or anything else that can cut ONE nickel thick.<< A bane indeed, not only for histotechs, but for those doing the grossing. Our big problem is breast biopsies. The combination of fat and fibrous tissue makes it hard (impossible) to get consistent thicknesses manually. I have been trying to find some kind 'jig' that would hold inked breast biopsies of widely varying sizes that could guide the knife as the biopsy is bread-loafed to get consistent thicknesses. I'm not sure what words to look for or search for. OTOH, given our difficulty in getting paid for the tech component (we are an independent lab, long used to global billing), I dont want to spend a fortune for something. All advice is super-greatly appreciated. Bill -- ______________ Bill Blank, MD Heartland Lab _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brendal.finlay <@t> medicalcenterclinic.com Wed Feb 13 11:06:55 2013 From: brendal.finlay <@t> medicalcenterclinic.com (Brendal Finlay) Date: Wed Feb 13 11:07:32 2013 Subject: [Histonet] (no subject) Message-ID: Hello everyone! I have a question for all of the histo techs out there.?It appears we are going to have to change some products and?we are in need of?some product recommendations.? Currently we use EM400 paraffin for embedding and infiltration.? Our department has tried to change paraffin before.? The paraffins tested did not work well for us so I would like to know if?anyone?can tell me a?comparable paraffin and from which vendor do you purchase? I may also need to change the blades that I use for microtomy.? I prefer the Surgipath / Leica high profile thin disposable blades (Item 3802123) and I am looking for?a similar blade.? Thank you! Brendal Finlay, HT (ASCP) From rjbuesa <@t> yahoo.com Wed Feb 13 11:09:08 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Feb 13 11:09:17 2013 Subject: [Histonet] (no subject) In-Reply-To: References: Message-ID: <1360775348.45354.YahooMailNeo@web163102.mail.bf1.yahoo.com> Paraplast and Feather from Sakura Ren? J. From: Brendal Finlay To: histonet@lists.utsouthwestern.edu Sent: Wednesday, February 13, 2013 12:06 PM Subject: [Histonet] (no subject) Hello everyone! I have a question for all of the histo techs out there.?It appears we are going to have to change some products and?we are in need of?some product recommendations.? Currently we use EM400 paraffin for embedding and infiltration.? Our department has tried to change paraffin before.? The paraffins tested did not work well for us so I would like to know if?anyone?can tell me a?comparable paraffin and from which vendor do you purchase? I may also need to change the blades that I use for microtomy.? I prefer the Surgipath / Leica high profile thin disposable blades (Item 3802123) and I am looking for?a similar blade.? Thank you! Brendal Finlay, HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mlunetta <@t> luhcares.org Wed Feb 13 11:21:21 2013 From: mlunetta <@t> luhcares.org (Matthew Lunetta) Date: Wed Feb 13 11:21:41 2013 Subject: [Histonet] 2013 Colorado Society of Histotechnology Meeting April 19th & 20th Message-ID: <170CACC1CA297947A168665B532DD05C21AD4627@EXLUH01.Ad.luhcares.org> The 2013 Colorado Society of Histotechnology meeting will be held April 19th & 20th at the La Quinta Inn, Fort Collins, CO. The program is now posted on the CSH website. For more information regarding the meeting, accommodations, online registration and payment, please visit http://www.coloradohisto.org/2013/meeting.htm. 2013 CSH Registration Packet: http://www.coloradohisto.org/2013/2013_CSH_Program.pdf Online registration is strongly encouraged. If you have to mail or fax your registration please print legibly (my eyes aren?t getting any younger) and provide a valid email address as receipts are no longer being mailed. If you are interested in attending the meeting please register sooner rather than later. As always, you have up until the day of the meeting to pay. Looking forward to seeing all of you at the meeting in April. Respectfully, Matt Lunetta BS HT(ASCP) Lead Histology Longmont United Hospital From bill501 <@t> mindspring.com Wed Feb 13 11:25:12 2013 From: bill501 <@t> mindspring.com (Bill B.) Date: Wed Feb 13 11:25:21 2013 Subject: [Histonet] grossing tools In-Reply-To: References: Message-ID: Hi Joyce, I will try that thanks. It would keep them from slipping around too. This has come up since we have gone from 2-3 breast biopsies a month to 5 to 10 a week, and me being a solo pathologist, I've been imagining devices to speed things up and be more accurate. Regards, Bill Blank At 4:09 PM +0000 2/13/13, Weems, Joyce K. wrote: >I have found squeezing the tissue (for the block sections) between two cassettes held back to back gives the firmness needed to trim good thin sections. > >Joyce Weems >Pathology Manager >678-843-7376 Phone >678-843-7831 Fax >joyce.weems@emoryhealthcare.org > > > >www.saintjosephsatlanta.org >5665 Peachtree Dunwoody Road >Atlanta, GA 30342 > >This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bill B. >Sent: Wednesday, February 13, 2013 9:58 AM >To: histonet@lists.utsouthwestern.edu >Subject: Re: [Histonet] grossing tools > >At 5:38 PM +0000 2/12/13, Bruce Gapinski wrote: >> I'm sure we are not the only histology lab that deals with thick grossed specimens. Has anyone tried the new gross tools by Sakura? Or anything else that can cut ONE nickel thick.<< > >A bane indeed, not only for histotechs, but for those doing the grossing. Our big problem is breast biopsies. The combination of fat and fibrous tissue makes it hard (impossible) to get consistent thicknesses manually. > >I have been trying to find some kind 'jig' that would hold inked breast biopsies of widely varying sizes that could guide the knife as the biopsy is bread-loafed to get consistent thicknesses. I'm not sure what words to look for or search for. > >OTOH, given our difficulty in getting paid for the tech component (we are an independent lab, long used to global billing), I dont want to spend a fortune for something. > >All advice is super-greatly appreciated. > >Bill > >-- >______________ >Bill Blank, MD >Heartland Lab > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >________________________________ > >This e-mail message (including any attachments) is for the sole use of >the intended recipient(s) and may contain confidential and privileged >information. If the reader of this message is not the intended >recipient, you are hereby notified that any dissemination, distribution >or copying of this message (including any attachments) is strictly >prohibited. > >If you have received this message in error, please contact >the sender by reply e-mail message and destroy all copies of the >original message (including attachments). > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brendal.finlay <@t> medicalcenterclinic.com Wed Feb 13 11:31:35 2013 From: brendal.finlay <@t> medicalcenterclinic.com (Brendal Finlay) Date: Wed Feb 13 11:32:10 2013 Subject: [Histonet] Re: Paraffin / Microtome Blades Message-ID: <7887847d972d29f4a7caace3c7f6abdf@medicalcenterclinic.com> Sorry about the lack of Subject...? I knew I was forgetting something! ;) Brendal Finlay, HT (ASCP) -----Original message----- From: Rene J Buesa rjbuesa@yahoo.com Date: Wed, 13 Feb 2013 12:09:08 -0600 To: Brendal Finlay brendal.finlay@medicalcenterclinic.com, "histonet@lists.utsouthwestern.edu" histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] (no subject) Paraplast and Feather from Sakura Ren? J. From: Brendal Finlay To: histonet@lists.utsouthwestern.edu Sent: Wednesday, February 13, 2013 12:06 PM Subject: [Histonet] (no subject) Hello everyone! I have a question for all of the histo techs out there.?It appears we are going to have to change some products and?we are in need of?some product recommendations.? Currently we use EM400 paraffin for embedding andinfiltration.? Our department has tried to change paraffin before.? The paraffins tested did not work well for us so I would like to know if?anyone?can tell me a?comparable paraffin and from which vendor do you purchase? I may also need to change the blades that I use for microtomy.? I prefer the Surgipath / Leica high profile thin disposable blades (Item 3802123) and I am looking for?a similar blade.? Thank you! Brendal Finlay, HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Sheila.Tapper <@t> essentiahealth.org Wed Feb 13 11:45:27 2013 From: Sheila.Tapper <@t> essentiahealth.org (Tapper, Sheila J.) Date: Wed Feb 13 11:45:37 2013 Subject: [Histonet] Benchmarking information Message-ID: <2A99E5980E5E0345B542E681A9A3F97804B52316@grouse.medcampus.org> We are working on a Lean/SixSigma project in our Histology Lab to decrease our TAT for pathology reports. Part of this process is to break down the total time and look at the different stages of the process to help identify where waste in the process is, such as: * Time from receipt in lab to delivery of slides to pathologists * Time special stains are ordered to delivery of slides to pathologists * Etc. Does anyone have any benchmarking information out there to share on this? Any help is appreciated. _________________________________________________ Sheila Tapper Anatomic Pathology Supervisor Essentia Health SMDC Laboratory Pathology 3W SMMC 407 East Third Street, Duluth, MN 55805 P: 218-786-5472 | F: 218-786-2369 sheila.tapper@essentiahealth.org From ewj <@t> pigsqq.org Wed Feb 13 12:05:04 2013 From: ewj <@t> pigsqq.org (=?UTF-8?B?IkUuIFdheW5lIEpvaG5zb24g5pyx56iz5qOuIg==?=) Date: Wed Feb 13 12:05:36 2013 Subject: [Histonet] grossing tools In-Reply-To: <1360775102.89849.YahooMailNeo@web163105.mail.bf1.yahoo.com> References: <1360775102.89849.YahooMailNeo@web163105.mail.bf1.yahoo.com> Message-ID: <511BD5D0.4080902@pigsqq.org> I intend to make us some of these tools. It's a great idea. On 3:59, Rene J Buesa wrote: > How much squeezing? Tissues have certain elasticity and after the squeezing they will "bounce back" as a thicker slice. "Free hand" sectioning I think is always better. > Ren? J. > > From: "Weems, Joyce K." > To: 'Bill B.'; "histonet@lists.utsouthwestern.edu" > Sent: Wednesday, February 13, 2013 11:09 AM > Subject: RE: [Histonet] grossing tools > > I have found squeezing the tissue (for the block sections) between two cassettes held back to back gives the firmness needed to trim good thin sections. > > Joyce Weems > Pathology Manager > 678-843-7376 Phone > 678-843-7831 Fax > joyce.weems@emoryhealthcare.org > > > > http://www.saintjosephsatlanta.org/ > 5665 Peachtree Dunwoody Road > Atlanta, GA 30342 > > This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bill B. > Sent: Wednesday, February 13, 2013 9:58 AM > To: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] grossing tools > > At 5:38 PM +0000 2/12/13, Bruce Gapinski wrote: > >> I'm sure we are not the only histology lab that deals with thick grossed specimens. Has anyone tried the new gross tools by Sakura? Or anything else that can cut ONE nickel thick.<< >> > A bane indeed, not only for histotechs, but for those doing the grossing. Our big problem is breast biopsies. The combination of fat and fibrous tissue makes it hard (impossible) to get consistent thicknesses manually. > > I have been trying to find some kind 'jig' that would hold inked breast biopsies of widely varying sizes that could guide the knife as the biopsy is bread-loafed to get consistent thicknesses. I'm not sure what words to look for or search for. > > OTOH, given our difficulty in getting paid for the tech component (we are an independent lab, long used to global billing), I dont want to spend a fortune for something. > > All advice is super-greatly appreciated. > > Bill > > -- > ______________ > Bill Blank, MD > Heartland Lab > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ________________________________ > > This e-mail message (including any attachments) is for the sole use of > the intended recipient(s) and may contain confidential and privileged > information. If the reader of this message is not the intended > recipient, you are hereby notified that any dissemination, distribution > or copying of this message (including any attachments) is strictly > prohibited. > > If you have received this message in error, please contact > the sender by reply e-mail message and destroy all copies of the > original message (including attachments). > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jqb7 <@t> cdc.gov Wed Feb 13 12:10:29 2013 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/OID/NCEZID)) Date: Wed Feb 13 12:10:44 2013 Subject: [Histonet] grossing tools In-Reply-To: <511BD5D0.4080902@pigsqq.org> References: <1360775102.89849.YahooMailNeo@web163105.mail.bf1.yahoo.com> <511BD5D0.4080902@pigsqq.org> Message-ID: I may be incorrect but I BEKLEIVE Dr. Azorides Morales at the Univ. of Miami designed the tools that Sakura manufactures. His is a huge teaching facility and I think they (pathologists, residents, etc.) all use them. (Could be wrong, but I seem to remember this a while back....) http://uhealthsystem.com/doctors/profile/1109 Jeanine H. Bartlett Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 404-639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of "E. Wayne Johnson ???" Sent: Wednesday, February 13, 2013 1:05 PM To: Rene J Buesa Cc: histonet@lists.utsouthwestern.edu; 'Bill B.' Subject: Re: Re: [Histonet] grossing tools I intend to make us some of these tools. It's a great idea. On 3:59, Rene J Buesa wrote: > How much squeezing? Tissues have certain elasticity and after the squeezing they will "bounce back" as a thicker slice. "Free hand" sectioning I think is always better. > Ren? J. > > From: "Weems, Joyce K." > To: 'Bill B.'; > "histonet@lists.utsouthwestern.edu" > Sent: Wednesday, February 13, 2013 11:09 AM > Subject: RE: [Histonet] grossing tools > > I have found squeezing the tissue (for the block sections) between two cassettes held back to back gives the firmness needed to trim good thin sections. > > Joyce Weems > Pathology Manager > 678-843-7376 Phone > 678-843-7831 Fax > joyce.weems@emoryhealthcare.org > > > > http://www.saintjosephsatlanta.org/ > 5665 Peachtree Dunwoody Road > Atlanta, GA 30342 > > This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bill B. > Sent: Wednesday, February 13, 2013 9:58 AM > To: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] grossing tools > > At 5:38 PM +0000 2/12/13, Bruce Gapinski wrote: > >> I'm sure we are not the only histology lab that deals with thick >> grossed specimens. Has anyone tried the new gross tools by Sakura? Or >> anything else that can cut ONE nickel thick.<< >> > A bane indeed, not only for histotechs, but for those doing the grossing. Our big problem is breast biopsies. The combination of fat and fibrous tissue makes it hard (impossible) to get consistent thicknesses manually. > > I have been trying to find some kind 'jig' that would hold inked breast biopsies of widely varying sizes that could guide the knife as the biopsy is bread-loafed to get consistent thicknesses. I'm not sure what words to look for or search for. > > OTOH, given our difficulty in getting paid for the tech component (we are an independent lab, long used to global billing), I dont want to spend a fortune for something. > > All advice is super-greatly appreciated. > > Bill > > -- > ______________ > Bill Blank, MD > Heartland Lab > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ________________________________ > > This e-mail message (including any attachments) is for the sole use of > the intended recipient(s) and may contain confidential and privileged > information. If the reader of this message is not the intended > recipient, you are hereby notified that any dissemination, > distribution or copying of this message (including any attachments) is > strictly prohibited. > > If you have received this message in error, please contact the sender > by reply e-mail message and destroy all copies of the original message > (including attachments). > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From allyse124 <@t> gmail.com Wed Feb 13 12:19:55 2013 From: allyse124 <@t> gmail.com (Allyse Mazzarelli) Date: Wed Feb 13 12:19:59 2013 Subject: [Histonet] Immuno Bed Plastic Question Message-ID: Hi histonetters! I have posted on this forum a while ago asking for opinions regardnig new types of plastics that work well with IHC. It was brought to my attention that "Immuno-Bed" (Polysciences) works rather well. I recently purchased the plastic, infiltrated/embedded well, and sectioned great, BUT, when I removed the excess GMA/rehydrated and labeled with my antibodies, NOTHING WORKED! Nothing showed up! Only DAPI!! The mechanism I used for removing GMA and rehydrating were as follows: 1.) 3 changes of 30 minutes each in 60 Celsius xylene. (Note: I recently let the slides sit in 60 Celsius xylene overnight too... but it did not work!!) 2.) 3 changes of 10 minutes each in 100% EtOH. 3.) 15 minutes in 95%, 75% & 50% EtOH 4.) 5 minutes in water 5.) 3 changes of 5 minutes each, PBS. I continued to block and apply my antibodies as normal. (Antibodies are all brand new, not duds!) Since this plastic was formulated specifically for IHC, I am at a loss. If anyone has used this plastic before, any insight directed my way would be extremely helpful!! Thanks, Allyse Mazzarelli Histologist NEUROTECH USA INC. From rjbuesa <@t> yahoo.com Wed Feb 13 12:45:41 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Feb 13 12:45:47 2013 Subject: [Histonet] Benchmarking information In-Reply-To: <2A99E5980E5E0345B542E681A9A3F97804B52316@grouse.medcampus.org> References: <2A99E5980E5E0345B542E681A9A3F97804B52316@grouse.medcampus.org> Message-ID: <1360781141.69430.YahooMailNeo@web163103.mail.bf1.yahoo.com> Sheila: Please go to http://www.histosearch.com/rene.html to find data on these subjects. Ren? J. From: "Tapper, Sheila J." To: histonet@lists.utsouthwestern.edu Sent: Wednesday, February 13, 2013 12:45 PM Subject: [Histonet] Benchmarking information We are working on a Lean/SixSigma project in our Histology Lab to decrease our TAT for pathology reports.? Part of this process is to break down the total time and look at the different stages of the process to help identify where waste in the process is, such as: *??? Time from receipt in lab to delivery of slides to pathologists *??? Time special stains are ordered to delivery of slides to pathologists? *??? Etc. Does anyone have any benchmarking information out there to share on this?? Any help is appreciated.? _________________________________________________ Sheila Tapper Anatomic Pathology Supervisor Essentia Health SMDC Laboratory Pathology 3W SMMC 407 East Third Street, Duluth, MN 55805 P: 218-786-5472 | F: 218-786-2369 sheila.tapper@essentiahealth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Wed Feb 13 12:47:09 2013 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/OID/NCEZID)) Date: Wed Feb 13 12:47:22 2013 Subject: [Histonet] grossing tools In-Reply-To: References: <1360775102.89849.YahooMailNeo@web163105.mail.bf1.yahoo.com> <511BD5D0.4080902@pigsqq.org> Message-ID: I have no idea how spell-check missed this but of course I meant to state, " I BELIEVE Dr........." -----Original Message----- From: Bartlett, Jeanine (CDC/OID/NCEZID) Sent: Wednesday, February 13, 2013 1:10 PM To: "E. Wayne Johnson ???"; Rene J Buesa Cc: histonet@lists.utsouthwestern.edu; 'Bill B.' Subject: RE: Re: [Histonet] grossing tools I may be incorrect but I BEKLEIVE Dr. Azorides Morales at the Univ. of Miami designed the tools that Sakura manufactures. His is a huge teaching facility and I think they (pathologists, residents, etc.) all use them. (Could be wrong, but I seem to remember this a while back....) http://uhealthsystem.com/doctors/profile/1109 Jeanine H. Bartlett Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 404-639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of "E. Wayne Johnson ???" Sent: Wednesday, February 13, 2013 1:05 PM To: Rene J Buesa Cc: histonet@lists.utsouthwestern.edu; 'Bill B.' Subject: Re: Re: [Histonet] grossing tools I intend to make us some of these tools. It's a great idea. On 3:59, Rene J Buesa wrote: > How much squeezing? Tissues have certain elasticity and after the squeezing they will "bounce back" as a thicker slice. "Free hand" sectioning I think is always better. > Ren? J. > > From: "Weems, Joyce K." > To: 'Bill B.'; > "histonet@lists.utsouthwestern.edu" > Sent: Wednesday, February 13, 2013 11:09 AM > Subject: RE: [Histonet] grossing tools > > I have found squeezing the tissue (for the block sections) between two cassettes held back to back gives the firmness needed to trim good thin sections. > > Joyce Weems > Pathology Manager > 678-843-7376 Phone > 678-843-7831 Fax > joyce.weems@emoryhealthcare.org > > > > http://www.saintjosephsatlanta.org/ > 5665 Peachtree Dunwoody Road > Atlanta, GA 30342 > > This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bill B. > Sent: Wednesday, February 13, 2013 9:58 AM > To: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] grossing tools > > At 5:38 PM +0000 2/12/13, Bruce Gapinski wrote: > >> I'm sure we are not the only histology lab that deals with thick >> grossed specimens. Has anyone tried the new gross tools by Sakura? Or >> anything else that can cut ONE nickel thick.<< >> > A bane indeed, not only for histotechs, but for those doing the grossing. Our big problem is breast biopsies. The combination of fat and fibrous tissue makes it hard (impossible) to get consistent thicknesses manually. > > I have been trying to find some kind 'jig' that would hold inked breast biopsies of widely varying sizes that could guide the knife as the biopsy is bread-loafed to get consistent thicknesses. I'm not sure what words to look for or search for. > > OTOH, given our difficulty in getting paid for the tech component (we are an independent lab, long used to global billing), I dont want to spend a fortune for something. > > All advice is super-greatly appreciated. > > Bill > > -- > ______________ > Bill Blank, MD > Heartland Lab > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ________________________________ > > This e-mail message (including any attachments) is for the sole use of > the intended recipient(s) and may contain confidential and privileged > information. If the reader of this message is not the intended > recipient, you are hereby notified that any dissemination, > distribution or copying of this message (including any attachments) is > strictly prohibited. > > If you have received this message in error, please contact the sender > by reply e-mail message and destroy all copies of the original message > (including attachments). > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Feb 13 12:48:27 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Feb 13 12:48:31 2013 Subject: [Histonet] Immuno Bed Plastic Question In-Reply-To: References: Message-ID: <1360781307.76698.YahooMailNeo@web163102.mail.bf1.yahoo.com> I think that you should bring this issue to the provider. For sure you will get a better answer than?bringing this issue to?HistoNet. Ren? J. From: Allyse Mazzarelli To: histonet@lists.utsouthwestern.edu Sent: Wednesday, February 13, 2013 1:19 PM Subject: [Histonet] Immuno Bed Plastic Question Hi histonetters! I have posted on this forum a while ago asking for opinions regardnig new types of plastics that work well with IHC. It was brought to my attention that "Immuno-Bed" (Polysciences) works rather well. I recently purchased the plastic, infiltrated/embedded well, and sectioned great, BUT, when I removed the excess GMA/rehydrated and labeled with my antibodies, NOTHING WORKED! Nothing showed up! Only DAPI!! The mechanism I used for removing GMA and rehydrating were as follows: 1.) 3 changes of 30 minutes each in 60 Celsius xylene. (Note: I recently let the slides sit in 60 Celsius xylene overnight too... but it did not work!!) 2.) 3 changes of 10 minutes each in 100% EtOH. 3.) 15 minutes in 95%, 75% & 50% EtOH 4.) 5 minutes in water 5.) 3 changes of 5 minutes each, PBS. I continued to block and apply my antibodies as normal. (Antibodies are all brand new, not duds!) Since this plastic was formulated specifically for IHC, I am at a loss. If anyone has used this plastic before, any insight directed my way would be extremely helpful!! Thanks, Allyse Mazzarelli Histologist NEUROTECH USA INC. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From robert_schoonhoven <@t> yahoo.com Wed Feb 13 12:57:03 2013 From: robert_schoonhoven <@t> yahoo.com (Robert Schoonhoven) Date: Wed Feb 13 12:57:11 2013 Subject: [Histonet] Immuno Bed Plastic Question In-Reply-To: References: Message-ID: <1360781823.29215.YahooMailNeo@web126206.mail.ne1.yahoo.com> Well..... If it is truly GMA then you will not be able to remove it.? What you describe is one of several methods to remove MMA.?? You will also lose the occasional section on exposure to the higher ( 70% +) grades of alcohol. ? If it is GMA I'd suggest you place your slides directly in PBS and then start your IHC procedure. Robert Schoonhoven, HT/HTL (ASCP) ________________________________ From: Allyse Mazzarelli To: histonet@lists.utsouthwestern.edu Sent: Wednesday, February 13, 2013 1:19 PM Subject: [Histonet] Immuno Bed Plastic Question Hi histonetters! I have posted on this forum a while ago asking for opinions regardnig new types of plastics that work well with IHC. It was brought to my attention that "Immuno-Bed" (Polysciences) works rather well. I recently purchased the plastic, infiltrated/embedded well, and sectioned great, BUT, when I removed the excess GMA/rehydrated and labeled with my antibodies, NOTHING WORKED! Nothing showed up! Only DAPI!! The mechanism I used for removing GMA and rehydrating were as follows: 1.) 3 changes of 30 minutes each in 60 Celsius xylene. (Note: I recently let the slides sit in 60 Celsius xylene overnight too... but it did not work!!) 2.) 3 changes of 10 minutes each in 100% EtOH. 3.) 15 minutes in 95%, 75% & 50% EtOH 4.) 5 minutes in water 5.) 3 changes of 5 minutes each, PBS. I continued to block and apply my antibodies as normal. (Antibodies are all brand new, not duds!) Since this plastic was formulated specifically for IHC, I am at a loss. If anyone has used this plastic before, any insight directed my way would be extremely helpful!! Thanks, Allyse Mazzarelli Histologist NEUROTECH USA INC. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PATRICIA.LENHART <@t> UCDENVER.EDU Wed Feb 13 13:03:07 2013 From: PATRICIA.LENHART <@t> UCDENVER.EDU (Lenhart, Patricia) Date: Wed Feb 13 13:03:16 2013 Subject: [Histonet] re: Immuno-Bed Message-ID: <1F70FCBB6D4EC549B2ADF69B9F9EAC036733CC8BBF@STEAMBOAT.ucdenver.pvt> Hi Allyse, Immuno-Bed is only recommended for lower molecular weight antibodies. Polyscience recommends using Osteo-Bed Bone Kit for the higher molecular weight ab's. Hope this helps. Pat Lenhart HT (ASCP) Univ. of Colorado Denver From gu.lang <@t> gmx.at Wed Feb 13 13:17:40 2013 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Wed Feb 13 13:17:48 2013 Subject: AW: [Histonet] Re: grossing tools In-Reply-To: References: Message-ID: <000f01ce0a1e$cb639200$622ab600$@gmx.at> I watched the video at the milestone-website. Has anyone so much time during grossing? And what about the next three breast-specimens waiting in the row. Who cleans this tool between the specimens? And what about feeling the tissue? I think my pathologists would get mad before making the first cut... ;-) Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Tom McNemar Gesendet: Mittwoch, 13. Februar 2013 16:18 An: 'Bartlett, Jeanine (CDC/OID/NCEZID)'; Bob Richmond; histonet@lists.utsouthwestern.edu Betreff: RE: [Histonet] Re: grossing tools We have a pair of the 3mm Cutmate forceps that we bought years ago for a particular pathologist but nobody uses them. If you go to the website below check out the Procut as well. http://www.milestonemed.com/histopathology/products.html Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/OID/NCEZID) Sent: Wednesday, February 13, 2013 9:57 AM To: Bob Richmond; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: grossing tools That is why I suggested having a rep. bring them out to demonstrate. "Sakura sells these. The grossing boards are items: 4800, 4801 and 4802. The grossing forks are 4803, 4804 and 4807. Ask your rep for a demo." Jeanine H. Bartlett Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 404-639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Wednesday, February 13, 2013 9:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: grossing tools Jeanine H. Bartlett at the CDC notes: >>Sakura sells these. The grossing boards are items: 4800, 4801 and 4802. The grossing forks are 4803, 4804 and 4807. Ask your rep for a demo. http://sakura.ifuture.com/sakura.asp?ifut=1000336642970309899%2CTKWK%3E6%3D% 3A5ST0 The prices of these tools are outrageous. The online catalog gives me no clue as to how they work. Bob Richmond Samurai Pathologist Maryville TN ************************************************ On Tue, Feb 12, 2013 at 8:01 PM, Bob Richmond wrote: > Bruce Gapinsk HT (ASCP), Chief Histologist, Marin Medical > Laboratories, PathGroup SF asks: > >>>Histonians, I'm sure we are not the only histology lab that deals >>>with thick grossed specimens. Has anyone tried the new gross tools by >>>Sakura? Or anything else that can cut ONE nickel thick. Tired of >>>reprocessing.<< > > This grumpy old (74) pathologist would be happy to try them out, > though I can cut freehand and almost never have a block reprocessed, > but the skill eludes a lot of the young folks. Do you have a Web link > for these tools? > > The problem would be getting a lab manager to spring for them. The > Laboratory Manager's Handy-Dandy Guide to Making Life Hard for the > Pathologist (I'm sure there is such a book, though I haven't actually > seen one) specifies that grossing is a ritual requiring only a basic > set of tools. > > One lab expected me to gross with an athame and chalice. I tried to > explain to them that OSHA and the CAP would not permit me to gross > skyclad. > > (You guys know I NEVER exaggerate.) > > Bob Richmond > Samurai Pathologist > Maryville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From alineumann <@t> aol.com Wed Feb 13 13:45:41 2013 From: alineumann <@t> aol.com (Alice Neumann) Date: Wed Feb 13 13:45:47 2013 Subject: [Histonet] Histology Laboratory Supervisor position In-Reply-To: <8CFD83846357328-DCC-147BE@webmail-d128.sysops.aol.com> References: <1360775102.89849.YahooMailNeo@web163105.mail.bf1.yahoo.com> <511BD5D0.4080902@pigsqq.org> <8CFD837A7079DA9-DCC-146E8@webmail-d128.sysops.aol.com> <8CFD83846357328-DCC-147BE@webmail-d128.sysops.aol.com> Message-ID: <8CFD839D6B61E0D-DCC-14B5A@webmail-d128.sysops.aol.com> TO APPLY: Please send resume to vsmith@pcgmolecular.com or fax to 678-928-9760. LOCATION: Dahlonega, Georgia 30533 SCHEDULE: Full Time Monday-Friday Day Shift Summary: Work involves repetitive laboratory tasks which require accuracy in the preparation of tissue blocks and slides, solvents, solutions, and compounds, and the routine maintenance and care of laboratory specimens, cultures, and equipment. Supervisory functions will include the process of hiring, training, competency assessments and performance appraisals. Additionally, will facilitate and develop continuing education programs, and provide backup for bench technicians in sectioning and staining as needed. Essential Job Duties: --Prepare tissues for processing, embedding, cutting, mounting and staining for microscopic examination. --Clean and maintain grossing room/area. --Assist with the daily grossing of specimens. --Perform appropriate staining of tissue. --Process body fluids for cytological examination. --Cover-slip slides appropriately. --Maintain and properly dispose of bio-hazardous waste. --Maintenance of policies, procedures and practices necessary to conduct the normal function of the histology laboratory. --Carry out routine duties and responsibilities with supervision. Make decisions and establishes work priorities on essential procedure-oriented operations. --Instruct laboratory personnel of functions that need to be maintained and performed. --Maintain maintenance records for laboratory equipment and ensures that all equipment is working. --Keep procedure manuals and other monthly/yearly records up to date according to CAP, CLIA and the State of Georgia in accordance with their standards and those of PCG Labs. --In charge of Histology inspections by CAP, CLIA and the State of Georgia. --Ensure that the daily functions of the laboratory are properly performed and improve the process if needed. Requirements: HT/HTL Certification Bachelor Degree in Biology or Science 5+ years of Experience as a Histotech LOCATION: Dahlonega, Georgia 30533 SCHEDULE: Full Time Monday-Friday Day Shift Summary: Work involves repetitive laboratory tasks which require accuracy in the preparation of tissue blocks and slides, solvents, solutions, and compounds, and the routine maintenance and care of laboratory specimens, cultures, and equipment. Supervisory functions will include the process of hiring, training, competency assessments and performance appraisals. Additionally, will facilitate and develop continuing education programs, and provide backup for bench technicians in sectioning and staining as needed. Essential Job Duties: --Prepare tissues for processing, embedding, cutting, mounting and staining for microscopic examination. --Clean and maintain grossing room/area. --Assist with the daily grossing of specimens. --Perform appropriate staining of tissue. --Process body fluids for cytological examination. --Cover-slip slides appropriately. --Maintain and properly dispose of bio-hazardous waste. --Maintenance of policies, procedures and practices necessary to conduct the normal function of the histology laboratory. --Carry out routine duties and responsibilities with supervision. Make decisions and establishes work priorities on essential procedure-oriented operations. --Instruct laboratory personnel of functions that need to be maintained and performed. --Maintain maintenance records for laboratory equipment and ensures that all equipment is working. --Keep procedure manuals and other monthly/yearly records up to date according to CAP, CLIA and the State of Georgia in accordance with their standards and those of PCG Labs. --In charge of Histology inspections by CAP, CLIA and the State of Georgia. --Ensure that the daily functions of the laboratory are properly performed and improve the process if needed. Requirements: HT/HTL Certification Bachelor Degree in Biology or Science 5+ years of Experience as a Histotech From pruegg <@t> ihctech.net Wed Feb 13 14:21:01 2013 From: pruegg <@t> ihctech.net (pruegg@ihctech.net) Date: Wed Feb 13 14:21:17 2013 Subject: [Histonet] cd8 on mouse tissue Message-ID: <008501ce0a27$a46c8220$ed458660$@ihctech.net> Is this still being done on frozen sections not aldehyde fixed or has someone figured out an antibody or method of doing it on FFPE mouse tissue yet??? Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E. Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 rueggihcconsultingpr@outlook.com This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. From Wanda.Smith <@t> HCAhealthcare.com Wed Feb 13 16:53:09 2013 From: Wanda.Smith <@t> HCAhealthcare.com (Wanda.Smith@HCAhealthcare.com) Date: Wed Feb 13 16:53:27 2013 Subject: [Histonet] unsubscribe Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BA27D1EE4A54@NADCWPMSGCMS03.hca.corpad.net> WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. From lwhite38 <@t> cogeco.ca Wed Feb 13 17:58:55 2013 From: lwhite38 <@t> cogeco.ca (Lori White) Date: Wed Feb 13 17:59:09 2013 Subject: [Histonet] Slide Mate printers Message-ID: <004801ce0a46$14d190f0$3e74b2d0$@ca> Hi, We recently installed Slide Mate printers and are experiencing poor print quality despite several onsite service calls. The vendor is attributing the problems to the slides we are using even though we are using Colorfrost slides from the manufacturer's List of Approved Slides. Has anybody successfully implemented these printers? If so, could you please share what slides you are using? Thanks in advance. Lori W Ontario, Canada From pablo.sanchez <@t> usc.es Thu Feb 14 03:36:54 2013 From: pablo.sanchez <@t> usc.es (Pablo Sanchez-Quinteiro) Date: Thu Feb 14 03:36:48 2013 Subject: [Histonet] Chromic Acid for Cleaning Glass Message-ID: <457439$l9rv1@correo2bi.usc.es> Dear Listers, I think that the use of Chromic Acid for cleaning the glassware is currently declined. Do you keep using it? Could you suggest me a non-comercial alternative? Thanks in advance, Pablo (Spain) From CThornton <@t> dahlchase.com Thu Feb 14 08:22:03 2013 From: CThornton <@t> dahlchase.com (Clare Thornton) Date: Thu Feb 14 08:22:13 2013 Subject: [Histonet] bone marrow specimens Message-ID: What fixative and decal solution is everyone using for bone marrow specimens which will have subsequent IHC and Kappa/Lambda ISH staining? We are currently using B+ fixative and Decal A (formic acid and formaldehyde). Our pathologists demand a quick turn around time, and are willing to sacrifice some quality for this, but we are having issues with our Kappa/Lambda ISH stains not highlighting all the plasma cells. The staining tends to be stronger on the outer edge of the tissue, and weakens or disappears entirely towards the middle, so I'm thinking the fixation and/or decal is the problem. We run our K/L ISH using Ventana instrumentation and reagents. Thank you in advance! Clare J. Thornton, HTL(ASCP)QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com From relia1 <@t> earthlink.net Thu Feb 14 08:51:14 2013 From: relia1 <@t> earthlink.net (Pam Barker) Date: Thu Feb 14 08:51:21 2013 Subject: [Histonet] RELIA Histology Careers Bulletin 2-14-2013 Happy Valentines Day! Message-ID: <00ae01ce0ac2$bd30aca0$379205e0$@earthlink.net> Hi Histonetters!! Happy Valentines' Day!!! I have some great histology opportunities to tell you about. Please feel free to take a second and peruse the list kind of the way one peruses an Assortment of chocolates in a heart shaped box! All of these are permanent full time positions and our clients offer excellent compensation, benefits and in some cases relocation and or sign on bonuses. My personal favorite is chocolate covered cherries!! How about you? Here is a list of my current openings!! Let me know if anything looks good! Management: Molecular Diagnostics Manager - Springfield OR Pathology Manager - San Francisco Bay Area Histology Supervisor - York, PA Lead IHC Tech - NYC, NY Lead Histotechnologist - Harrisonburg, VA Lead Grossing Histotechnologist - Chattanooga, TN HT/HTL Opportunities: San Francisco, CA Collegeville, PA Louisville, KY Charlotte, NC Portland, ME Concord, NH If you are interested in any of these positions please call me at 866-607-3542 or e-mail me at relia1@earthlink.net If you would like you can e-mail me your resume and a number where I can reach you at a time that is convenient for you. If you are interested in looking into new job opportunities in other areas that are not mentioned above please contact me as well. I work with facilities nationwide and I will keep your resume confidential. I will only represent you to jobs you tell me you are interested in looking into. Remember. It never hurts to keep an eye open even if you are happy in your present job. Valentines' Day Special.If you refer someone that I place I will pay you a 500 dollar referral fee and during the month of February I will throw in a box of Godiva Chocolates!!! :-) Thank you, Pam - 866-607-3542 (866-60RELIA) Right Place, Right Time, Right Move with RELIA! Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From brannon <@t> alliedsearchpartners.com Thu Feb 14 09:21:44 2013 From: brannon <@t> alliedsearchpartners.com (Brannon Owens) Date: Thu Feb 14 09:21:56 2013 Subject: [Histonet] Technical Coordinator of Histology Opening Near Fort Myers, FL Message-ID: Position: Technical Coordinator of Histology Schedule: Monday-Friday Day Shift Location: Fort Myers, FL IHC experience is a must! Email Brannon@alliedsearchpartners.com for a full job description. To view a complete list of Allied Search Partners current openings go to: http://www.alliedsearchpartners.com/careers.php -- Brannon Owens Recruitment Manager Allied Search Partners From rjbuesa <@t> yahoo.com Thu Feb 14 09:36:24 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Feb 14 09:36:29 2013 Subject: [Histonet] bone marrow specimens In-Reply-To: References: Message-ID: <1360856184.41346.YahooMailNeo@web163102.mail.bf1.yahoo.com> I fix in NBF at pH7 exactly and decalcify with EDTA Ren? J. From: Clare Thornton To: "histonet@lists.utsouthwestern.edu" Sent: Thursday, February 14, 2013 9:22 AM Subject: [Histonet] bone marrow specimens What fixative and decal solution is everyone using for bone marrow specimens which will have subsequent IHC and Kappa/Lambda ISH staining?? We are currently using B+ fixative and Decal A (formic acid and formaldehyde).? Our pathologists demand a quick turn around time, and are willing to sacrifice some quality for this, but we are having issues with our Kappa/Lambda ISH stains not highlighting all the plasma cells.? The staining tends to be stronger on the outer edge of the tissue, and weakens or disappears entirely towards the middle, so I'm thinking the fixation and/or decal is the problem.? We run our K/L ISH using Ventana instrumentation and reagents. Thank you in advance! Clare J. Thornton, HTL(ASCP)QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Thu Feb 14 10:01:16 2013 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Thu Feb 14 10:01:34 2013 Subject: [Histonet] tissue processor remote alarm systems Message-ID: <761E2B5697F795489C8710BCC72141FF055348@ex07.net.ucsf.edu> I'd like to hear from labs that use a remote messaging system for their VIP5 tissue processors that are left unattended overnight. One I saw on old histonet posts is Sensaphone. I am wondering what kind of information you can get from the system. Does it just say there is a problem but no details? Can you connect several processors to one sensing unit? Can it tell the difference between the units (ie, processor 1 vs processor 4?). Thanks for any info! Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center From histopatty <@t> aol.com Thu Feb 14 10:02:23 2013 From: histopatty <@t> aol.com (Histopatty) Date: Thu Feb 14 10:02:31 2013 Subject: [Histonet] standard protocol Message-ID: <8CFD8E3CEBD8C39-130C-23C72@webmail-d142.sysops.aol.com> We are getting instructions from a pharmaceutical company who needs slides, with instructions stating "sections should be floated per standard protocol, xylene dipped from 3 minutes and dried for 10 minutes." I'm reaching out to those of you who routinly deal with research protocols and wondering is the "standard protocol" the normal float, dri, melt? Patricia Eneff for Kay Pierce Please respond to sharon.pierce@hcahealthcare.com if possilbe. From TGoins <@t> mt.gov Thu Feb 14 10:12:09 2013 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Thu Feb 14 10:12:28 2013 Subject: [Histonet] standard protocol In-Reply-To: <8CFD8E3CEBD8C39-130C-23C72@webmail-d142.sysops.aol.com> References: <8CFD8E3CEBD8C39-130C-23C72@webmail-d142.sysops.aol.com> Message-ID: Ask the pharmaceutical company for detailed instructions, not vague references. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Histopatty Sent: Thursday, February 14, 2013 9:02 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] standard protocol We are getting instructions from a pharmaceutical company who needs slides, with instructions stating "sections should be floated per standard protocol, xylene dipped from 3 minutes and dried for 10 minutes." I'm reaching out to those of you who routinly deal with research protocols and wondering is the "standard protocol" the normal float, dri, melt? Patricia Eneff for Kay Pierce Please respond to sharon.pierce@hcahealthcare.com if possilbe. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Feb 14 10:28:44 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Feb 14 10:28:53 2013 Subject: [Histonet] standard protocol In-Reply-To: <8CFD8E3CEBD8C39-130C-23C72@webmail-d142.sysops.aol.com> References: <8CFD8E3CEBD8C39-130C-23C72@webmail-d142.sysops.aol.com> Message-ID: <1360859324.87893.YahooMailNeo@web163106.mail.bf1.yahoo.com> "Standard" protocol is a very "encompassing" word and implies that it is "standard" for all labs and there is no such standard. Each lab has its own (although sometimes adhering to some general consensus) "standard" protocol, so probably that pharmaceutical company is referring to their "standard" protocol. Why don't you ask them to send you their "standard" protocol. Generally speaking your section will be floated and then the slide is tilted to assure the water totally draining from behind the section and are oven dried after and that is all. I do not see any advantage in dewaxing (=dipped for 3 minutes in xylene) and dried again. It even may be damaging to the section being exposed "naked" (without paraffin) to air. There has to be some error in those instructions by somebody not quite familiar with histology, as is usually the norm when dealing with pharmaceutical investigators. Ask them about these "non histological" instructions. Ren? J. From: Histopatty To: histonet@lists.utsouthwestern.edu Sent: Thursday, February 14, 2013 11:02 AM Subject: [Histonet] standard protocol We are getting instructions from a pharmaceutical company who needs slides, with instructions stating "sections should be floated per standard protocol, xylene dipped from 3 minutes and dried for 10 minutes."? I'm reaching out to those of you who routinly deal with research protocols and wondering is the "standard protocol" the normal float, dri, melt? Patricia Eneff for Kay Pierce Please respond to sharon.pierce@hcahealthcare.com if possilbe.? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Nancy_Schmitt <@t> pa-ucl.com Thu Feb 14 10:30:44 2013 From: Nancy_Schmitt <@t> pa-ucl.com (Nancy Schmitt) Date: Thu Feb 14 10:30:48 2013 Subject: [Histonet] General Data Message-ID: <906B4DA90ED1DB4DB6C7E94D7CEE6C364DC6ECB4@PEITHA.wad.pa-ucl.com> Good Morning- We are currently using General Data for cassette labeling - very happy with it. The next phase is implementing slide labeling. I would appreciate knowing what kind of setup people are using at the microtome - laptop? touchpad? Full computer? Is the arm off of the microtome or out from the counter? Any and all information is appreciated! Nancy Schmitt, HT, MLT(ASCP) Histology Coordinator Dubuque, IA 52001 NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From kenneth.metzger <@t> aruplab.com Thu Feb 14 10:31:53 2013 From: kenneth.metzger <@t> aruplab.com (Metzger, Kenneth) Date: Thu Feb 14 10:32:04 2013 Subject: [Histonet] "Vacuole" appearance in lymph Nodes Message-ID: <3855827CD3E36249A30D57F6F896F8F17CC022A7@EXMBX2.aruplab.net> Hi All, I have sent this question to respected colleagues individually so pardon my redundancy if you have this email already. Lately we have had random nodes show up with areas that appear to have multiple vacuoles through-out the specimen. They have different patterns section to section so I know it is not in the tissue. The staining looks fine and the tissue cuts well so I don't think it is a processing issue. Any ideas on the problem would be welcome. Thanks Ken Kenneth G Metzger HTL(ASCP) Histology Supervisor ARUP Labs Salt Lake City, Utah Phone: (801)583-2787 ext. 3101 Fax: (801) 584-5244 Email: kenneth.metzger@aruplab.com ------------------------------------------------------------------- The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws, or protected health information and is intended only for the use of the recipient. Unauthorized forwarding, printing, copying, distributing, or use of such information is strictly prohibited and may be unlawful. If you are not the intended recipient, please promptly delete this e-mail and notify the sender of the delivery error or you may call ARUP Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 (800) 522-2787 ext. 2100 From Timothy.Morken <@t> ucsfmedctr.org Thu Feb 14 10:37:39 2013 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Thu Feb 14 10:38:31 2013 Subject: [Histonet] RE: General Data In-Reply-To: <906B4DA90ED1DB4DB6C7E94D7CEE6C364DC6ECB4@PEITHA.wad.pa-ucl.com> References: <906B4DA90ED1DB4DB6C7E94D7CEE6C364DC6ECB4@PEITHA.wad.pa-ucl.com> Message-ID: <761E2B5697F795489C8710BCC72141FF0553D2@ex07.net.ucsf.edu> Nancy, what kind of system are you going to use - a barcoded tracking system to scan blocks at the microtome, or do you have to type in case info to get the labels? Tim Morken UCSF Pathology -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt Sent: Thursday, February 14, 2013 8:31 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] General Data Good Morning- We are currently using General Data for cassette labeling - very happy with it. The next phase is implementing slide labeling. I would appreciate knowing what kind of setup people are using at the microtome - laptop? touchpad? Full computer? Is the arm off of the microtome or out from the counter? Any and all information is appreciated! Nancy Schmitt, HT, MLT(ASCP) Histology Coordinator Dubuque, IA 52001 NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jo-ann.bader <@t> mcgill.ca Thu Feb 14 10:49:38 2013 From: jo-ann.bader <@t> mcgill.ca (Jo-Ann Bader, Ms.) Date: Thu Feb 14 10:50:33 2013 Subject: [Histonet] CD31 problewms Message-ID: <8C36045F0065CE48906E684F15FD4CB60AAD02@EXMBX2010-6.campus.MCGILL.CA> We are having problems getting our CD31 to work well on our NBF fixed mouse tissue. There is staining but it is very pale. The CD31 comes from Bio-care and we are are using the IntelliPATH stainer. We have tried: - Trypsine 37C 15 min / Trypsine RT 30 min - Trypsine (1:2 and 1:3) - With Decloaker / without decloaker - DAB Sparkle / No DAB sparkle - Different dilution of CD31 (1:50 / 1:25) - Different time of CD31 incubation (2h / 3h) - Different time of incubation of secondary and tertiary reagent (10min to 20min) Thanks Jo-Ann Bader Histology Co-Ordinator Goodman Cancer Centre McGill University 1160 Pine Ave. W - Rm 312 Montreal, QC, Canada H3G 1Y6 Tel: 514-398-8270 From rjbuesa <@t> yahoo.com Thu Feb 14 11:27:22 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Feb 14 11:27:34 2013 Subject: [Histonet] CD31 problewms In-Reply-To: <8C36045F0065CE48906E684F15FD4CB60AAD02@EXMBX2010-6.campus.MCGILL.CA> References: <8C36045F0065CE48906E684F15FD4CB60AAD02@EXMBX2010-6.campus.MCGILL.CA> Message-ID: <1360862842.89214.YahooMailNeo@web163102.mail.bf1.yahoo.com> If you have not changed the Ab provider nor any step on your protocol, check the Ig concentration in the lot you are using now compared with the lot you used to determine the Ab dilution. If the Ig is less concentrated?now, you may need to increase the concentration (reduce the dilution). Ren? J. ? From: "Jo-Ann Bader, Ms." To: "histonet@lists.utsouthwestern.edu" Sent: Thursday, February 14, 2013 11:49 AM Subject: [Histonet] CD31 problewms We are having problems getting our CD31 to work well on our NBF fixed mouse tissue. There is staining but it is very pale. The CD31 comes from Bio-care and we are are using the IntelliPATH stainer. We have tried: - Trypsine 37C 15 min / Trypsine RT 30 min - Trypsine (1:2 and 1:3) - With Decloaker / without decloaker - DAB Sparkle / No DAB sparkle - Different dilution of CD31 (1:50 / 1:25) - Different time of CD31 incubation (2h / 3h) - Different time of incubation of secondary and tertiary reagent (10min to 20min) Thanks Jo-Ann Bader Histology Co-Ordinator Goodman Cancer Centre McGill University 1160 Pine Ave. W - Rm 312 Montreal, QC, Canada H3G 1Y6 Tel: 514-398-8270 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Dennis.Hahn <@t> cookchildrens.org Thu Feb 14 11:29:05 2013 From: Dennis.Hahn <@t> cookchildrens.org (Dennis Hahn) Date: Thu Feb 14 11:29:12 2013 Subject: [Histonet] Open Position Message-ID: Cook Childrens Medical Center in Ft Worth, TX has an immediate day shift opening for a full time HT (ASCP). Interested candidates must apply online at www.cookchildrens.org and meet one of the below qualifications listed at the online job posting to be considered. Experience in manual IHC and special staining preferred. QUALIFICATIONS: High school graduate with 5 years on the job training and 5 years experience in a high complexity histology lab. Bachelors degree in Medical Technology or an appropriate biological, chemical, or physical science from an accredited program. Associate degree in laboratory science or histology from an accredited institution. Experience in high complexity histology lab preferred. Candidate must be certified by the American Society of Clinical Pathologists or equivalent nationally recognized certifying agency, (HT/ASCP) (HTL/ASCP), or is eligible for certification. Registry eligible candidates must pass certifying registry within 3 months of employment. Please feel free to contact me if you have any questions. Dennis Dennis Hahn, HT (ASCP) Histology Lab Supervisor Cook Children's Medical Center 801 7th Avenue Ft. Worth, TX 76104 (682) 885-6168 From bethcoxx <@t> gmail.com Thu Feb 14 11:46:43 2013 From: bethcoxx <@t> gmail.com (Beth Cox) Date: Thu Feb 14 11:46:49 2013 Subject: [Histonet] RE: SlideMate Printers Message-ID: Hi Lori, We have been trying to get the SlideMate/ Printmate system working in our lab for close to a year now. Our experience with the SlideMates has been dismal. Our quality with the Slidemates is minimally adequate, with a lot of 'misprints'. We didn't get the best print with their Colorfrost slides, in fact we have found the Statlab 'ColorView' slides to be the least problematic (note: I did not say the results are good, just less bad). Within 6 months of use, we needed to send all three of our Slidemates back to get new printheads, and a cost of $1000 each. I would not consider our implementation 'successful', and I would not have purchased them if I knew then what I know now! Beth Cox, HTL/SCT(ASCP)QIHC Histology Supervisor St Elizabeth Healthcare, Edgewood, KY Message: 13 Date: Wed, 13 Feb 2013 18:58:55 -0500 From: "Lori White" Subject: [Histonet] Slide Mate printers To: Message-ID: <004801ce0a46$14d190f0$3e74b2d0$@ca> Content-Type: text/plain; charset="us-ascii" Hi, We recently installed Slide Mate printers and are experiencing poor print quality despite several onsite service calls. The vendor is attributing the problems to the slides we are using even though we are using Colorfrost slides from the manufacturer's List of Approved Slides. Has anybody successfully implemented these printers? If so, could you please share what slides you are using? Thanks in advance. Lori W Ontario, Canada From vtobias <@t> uw.edu Thu Feb 14 11:50:52 2013 From: vtobias <@t> uw.edu (Victor A. Tobias) Date: Thu Feb 14 11:51:15 2013 Subject: [Histonet] RE: SlideMate Printers In-Reply-To: References: Message-ID: We had a demo of the new Sakura Slide printer and it is awesome compared to the Slide Mate. Victor Victor Tobias HT(ASCP) Clinical Applications Analyst Harborview Medical Center Dept of Pathology Room NJB244 Seattle, WA 98104 vtobias@u.washington.edu 206-744-2735 206-744-8240 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Beth Cox Sent: Thursday, February 14, 2013 9:47 AM To: histonet@lists.utsouthwestern.edu Cc: lwhite38@cogeco.ca Subject: [Histonet] RE: SlideMate Printers Hi Lori, We have been trying to get the SlideMate/ Printmate system working in our lab for close to a year now. Our experience with the SlideMates has been dismal. Our quality with the Slidemates is minimally adequate, with a lot of 'misprints'. We didn't get the best print with their Colorfrost slides, in fact we have found the Statlab 'ColorView' slides to be the least problematic (note: I did not say the results are good, just less bad). Within 6 months of use, we needed to send all three of our Slidemates back to get new printheads, and a cost of $1000 each. I would not consider our implementation 'successful', and I would not have purchased them if I knew then what I know now! Beth Cox, HTL/SCT(ASCP)QIHC Histology Supervisor St Elizabeth Healthcare, Edgewood, KY Message: 13 Date: Wed, 13 Feb 2013 18:58:55 -0500 From: "Lori White" Subject: [Histonet] Slide Mate printers To: Message-ID: <004801ce0a46$14d190f0$3e74b2d0$@ca> Content-Type: text/plain; charset="us-ascii" Hi, We recently installed Slide Mate printers and are experiencing poor print quality despite several onsite service calls. The vendor is attributing the problems to the slides we are using even though we are using Colorfrost slides from the manufacturer's List of Approved Slides. Has anybody successfully implemented these printers? If so, could you please share what slides you are using? Thanks in advance. Lori W Ontario, Canada _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brett_connolly <@t> merck.com Thu Feb 14 11:53:44 2013 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Thu Feb 14 11:53:52 2013 Subject: [Histonet] CD31 problewms In-Reply-To: <1360862842.89214.YahooMailNeo@web163102.mail.bf1.yahoo.com> References: <8C36045F0065CE48906E684F15FD4CB60AAD02@EXMBX2010-6.campus.MCGILL.CA> <1360862842.89214.YahooMailNeo@web163102.mail.bf1.yahoo.com> Message-ID: I think the CD31 Ab from Dianova is what works best for most people on FFPE mouse tissue. Brett Brett M. Connolly, Ph.D. Principal Scientist, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, February 14, 2013 12:27 PM To: Jo-Ann Bader, Ms.; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] CD31 problewms If you have not changed the Ab provider nor any step on your protocol, check the Ig concentration in the lot you are using now compared with the lot you used to determine the Ab dilution. If the Ig is less concentrated?now, you may need to increase the concentration (reduce the dilution). Ren? J. ? From: "Jo-Ann Bader, Ms." To: "histonet@lists.utsouthwestern.edu" Sent: Thursday, February 14, 2013 11:49 AM Subject: [Histonet] CD31 problewms We are having problems getting our CD31 to work well on our NBF fixed mouse tissue. There is staining but it is very pale. The CD31 comes from Bio-care and we are are using the IntelliPATH stainer. We have tried: - Trypsine 37C 15 min / Trypsine RT 30 min - Trypsine (1:2 and 1:3) - With Decloaker / without decloaker - DAB Sparkle / No DAB sparkle - Different dilution of CD31 (1:50 / 1:25) - Different time of CD31 incubation (2h / 3h) - Different time of incubation of secondary and tertiary reagent (10min to 20min) Thanks Jo-Ann Bader Histology Co-Ordinator Goodman Cancer Centre McGill University 1160 Pine Ave. W - Rm 312 Montreal, QC, Canada H3G 1Y6 Tel: 514-398-8270 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From TMcNemar <@t> lmhealth.org Thu Feb 14 12:20:43 2013 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Thu Feb 14 12:20:45 2013 Subject: [Histonet] RE: General Data In-Reply-To: <906B4DA90ED1DB4DB6C7E94D7CEE6C364DC6ECB4@PEITHA.wad.pa-ucl.com> References: <906B4DA90ED1DB4DB6C7E94D7CEE6C364DC6ECB4@PEITHA.wad.pa-ucl.com> Message-ID: We use a full PC mounted under the counter and a touchpad mounted on an arm that can be positioned just about anyplace. The printer is mounted on a small shelf below the counter. Small scanner mounted to a desktop stand that can be moved wherever the cutter wants. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt Sent: Thursday, February 14, 2013 11:31 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] General Data Good Morning- We are currently using General Data for cassette labeling - very happy with it. The next phase is implementing slide labeling. I would appreciate knowing what kind of setup people are using at the microtome - laptop? touchpad? Full computer? Is the arm off of the microtome or out from the counter? Any and all information is appreciated! Nancy Schmitt, HT, MLT(ASCP) Histology Coordinator Dubuque, IA 52001 NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From Lisa.White3 <@t> va.gov Thu Feb 14 12:18:01 2013 From: Lisa.White3 <@t> va.gov (White, Lisa M.) Date: Thu Feb 14 12:23:33 2013 Subject: [Histonet] Slide Mate printers Message-ID: <2B2ECF33934F5D4996D8BE03EFDF39760AE81963@VHAV09MSGA3.v09.med.va.gov> We have the slide mate printers. We have the best luck with Item# 12 550 15 Slide Superfrost White (there are color slides also). I have found that you have to remove excess used printer ribbon from the unit at least once a week. One day all my prefixes disappeared from my slides, investigated to find that the used ribbon roll had started leaning to one side. Removed the old ribbon and it started printing well after that. We had some trouble with format, but after a service call it has been resolved. If you think that printer head is expensive to repair check out some of the other ones many many $$$$$ more than that. Lisa White, HT(ASCP) From mjdessoye <@t> commonwealthhealth.net Thu Feb 14 12:55:10 2013 From: mjdessoye <@t> commonwealthhealth.net (Dessoye, Michael J) Date: Thu Feb 14 12:55:25 2013 Subject: [Histonet] tissue processor remote alarm systems In-Reply-To: <761E2B5697F795489C8710BCC72141FF055348@ex07.net.ucsf.edu> Message-ID: I have a Sensaphone unit. It can handle four different instruments. When it calls it will give you the phone number it's calling from, the sensor (instrument) number, and the 'alert condition' (there are several...for example 1 is power failure, etc.) Any other questions about it I'm happy to help. Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdessoye@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 -----Original Message----- From: Morken, Timothy [mailto:Timothy.Morken@ucsfmedctr.org] Sent: Thursday, February 14, 2013 11:01 AM To: Histonet Subject: [Histonet] tissue processor remote alarm systems I'd like to hear from labs that use a remote messaging system for their VIP5 tissue processors that are left unattended overnight. One I saw on old histonet posts is Sensaphone. I am wondering what kind of information you can get from the system. Does it just say there is a problem but no details? Can you connect several processors to one sensing unit? Can it tell the difference between the units (ie, processor 1 vs processor 4?). Thanks for any info! Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Commonwealth Health. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. From mucram11 <@t> comcast.net Thu Feb 14 13:01:36 2013 From: mucram11 <@t> comcast.net (Pam Marcum) Date: Thu Feb 14 13:01:45 2013 Subject: [Histonet] Slide Mate printers In-Reply-To: <2B2ECF33934F5D4996D8BE03EFDF39760AE81963@VHAV09MSGA3.v09.med.va.gov> Message-ID: <574997531.249514.1360868496256.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> We bought the Print Mate for Cassettes and Slide Mate for slides over two years ago.? We use the Thermo Colorfrost slides and they work well.? We had issues in t he beginning and it was very difficult.? Thermo hung in there and made sure we were happy and got things working well.? We are very happy overall.? It was a newer system when we got it and the problems were well handled.? The ribbon can be an issue especially if you are not paying attention and too much is on t he used spindle but also checking to prevent the ribbon from pulling the foil at the end of a roll under as that will damage t he print head.? It can also be placed in the printer wrong and if it is not tight enough and allowed to bunch up the printing is bad. Pam Marcum ----- Original Message ----- From: "Lisa M. White" To: histonet@lists.utsouthwestern.edu Sent: Thursday, February 14, 2013 12:18:01 PM Subject: [Histonet] Slide Mate printers We have the slide mate printers. ?We have the best luck with Item# 12 550 15 Slide Superfrost White (there are color slides also). I have found that you have to remove excess used printer ribbon from the unit at least once a week. ?One day all my prefixes disappeared from my slides, investigated to find that the used ribbon roll had started leaning to one side. ?Removed the old ribbon and it started printing well after that. ?We had some trouble with format, but after a service call it has been resolved. ? If you think that printer head is expensive to repair check out some of the other ones many many $$$$$ more than that. ? ? Lisa White, HT(ASCP) ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Ronald.Houston <@t> nationwidechildrens.org Thu Feb 14 13:08:22 2013 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Thu Feb 14 13:08:29 2013 Subject: [Histonet] FISH baking and dewaxing Message-ID: Can someone please explain why paraffin-embedded slides for FISH need to be baked for so long and have extensive dewaxing in xylenes and subsequent alcohol pretreatment? Our Molecular group bake for >1 hr at 65C, and then treat sections in 3 changes of xylene 15 min each, and 100% ethanol 15 minutes each x2 before air-drying. I have asked them why so long and get the standard response -- "that's the way we always do it"! Thanks Ronnie Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org "One person with passion is better than forty people merely interested." ~ E.M. Forster From marktarango <@t> gmail.com Thu Feb 14 13:29:51 2013 From: marktarango <@t> gmail.com (Mark Tarango) Date: Thu Feb 14 13:29:54 2013 Subject: [Histonet] FISH baking and dewaxing In-Reply-To: References: Message-ID: Hi Ronnie, FISH pretreatment can be harsh and that might explain the long baking (don't want the tissue to fall off). On the other hand, I've put a slide in the oven for 5 minutes and had the tissue stay on in a rush. I think the dewaxing can be shortened without affecting the FISH signals. Our molecular director has us validate any minor change to the pretreatment protocol, so depending on the director's opinion it could mean a re-validation. Mark T. On Thu, Feb 14, 2013 at 11:08 AM, Houston, Ronald < Ronald.Houston@nationwidechildrens.org> wrote: > Can someone please explain why paraffin-embedded slides for FISH need to > be baked for so long and have extensive dewaxing in xylenes and subsequent > alcohol pretreatment? > Our Molecular group bake for >1 hr at 65C, and then treat sections in 3 > changes of xylene 15 min each, and 100% ethanol 15 minutes each x2 before > air-drying. I have asked them why so long and get the standard response -- > "that's the way we always do it"! > > Thanks > Ronnie > > Ronnie Houston, MS HT(ASCP)QIHC > Anatomic Pathology Manager > ChildLab, a Division of Nationwide Children's Hospital > www.childlab.com > > 700 Children's Drive > Columbus, OH 43205 > (P) 614-722-5450 > (F) 614-722-2899 > ronald.houston@nationwidechildrens.org ronald.houston@nationwidechildrens.org> > www.NationwideChildrens.org > > "One person with passion is better than forty people merely interested." > ~ E.M. Forster > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From minniesann <@t> hotmail.com Thu Feb 14 13:51:52 2013 From: minniesann <@t> hotmail.com (Maribel Santiago) Date: Thu Feb 14 13:51:58 2013 Subject: [Histonet] H&E Destain protocol Message-ID: Hello Histonet, I have samples that were stained during fixation with Eosin. First question, since I will do IHC on them, do I need to get rid of the eosin like you do with an already H&E stained slide? If so, that brings me to my second question, does anyone have a protocol that are willing to share with me along with helpful hints?? Thanks,Minnie From pruegg <@t> ihctech.net Thu Feb 14 14:31:54 2013 From: pruegg <@t> ihctech.net (pruegg@ihctech.net) Date: Thu Feb 14 14:32:36 2013 Subject: [Histonet] zinc salt fixed mouse tissue PE stained for CD4/cd8 Message-ID: <008001ce0af2$54619dd0$fd24d970$@ihctech.net> Has anyone tried this: zinc salt fixed mouse tissue PE stained for CD4/cd8 I found some papers that look impressive. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E. Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 rueggihcconsultingpr@outlook.com This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. From Helena.Howe <@t> ProMedica.org Thu Feb 14 15:48:50 2013 From: Helena.Howe <@t> ProMedica.org (Howe, Helena) Date: Thu Feb 14 15:48:59 2013 Subject: [Histonet] Monitor & Key Board Mounts Message-ID: <5288C492C713554297913A98A660A539021EB8E242@phsimailcr03.phsi.promedica.org> I need to order Monitor & Keyboard Mounts for our custom made Grossing Stations. Need suggestions the standard ones that com with Gross Stations are very expensive. Helena Howe ProMedica Laboratories _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ EMAIL CONFIDENTIALITY NOTICE This Email message, and any attachments, may contain confidential patient health information that is legally protected. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this message is strictly prohibited. If you have received this information in error, please notify the sender immediately by replying to this message and delete the message from your system. From SHEILA.HERRINGTON <@t> interiorhealth.ca Thu Feb 14 16:16:48 2013 From: SHEILA.HERRINGTON <@t> interiorhealth.ca (HERRINGTON, SHEILA) Date: Thu Feb 14 16:17:44 2013 Subject: [Histonet] H&E Destain protocol In-Reply-To: References: Message-ID: <9D5F3F245A5FE0458E46E9A71E3EE08C06AB0F7569@DC1SERV352.interiorhealth.ca> Not if it is just from processing. Eosin from processing will not affect any IHC or Special stains. If it was H&E stained you just need to take back through Xylene, alcohol to water. The alcohol will remove some of the eosin, and the IHC process will not be affected by the stains. It will simply replace them. Usually I do the same process as well with a control just to quality control it. Sheila Herrington, IHC Kelowna General Grade 3, Immunohistochemistry, Histolopathogy Kelowna General Hospital -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Maribel Santiago Sent: Thursday, February 14, 2013 11:52 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] H&E Destain protocol Hello Histonet, I have samples that were stained during fixation with Eosin. First question, since I will do IHC on them, do I need to get rid of the eosin like you do with an already H&E stained slide? If so, that brings me to my second question, does anyone have a protocol that are willing to share with me along with helpful hints?? Thanks,Minnie _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis <@t> bresnan.net Thu Feb 14 17:20:27 2013 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Thu Feb 14 17:21:52 2013 Subject: [Histonet] FW: CD4 CD8 Zinc Tris PE staining In-Reply-To: <000401ce0b02$7ceba060$76c2e120$@bresnan.net> References: <000401ce0b02$7ceba060$76c2e120$@bresnan.net> Message-ID: <001801ce0b09$e182a5d0$a487f170$@bresnan.net> Hello Patsy, You wrote: Has anyone tried this: zinc salt fixed mouse tissue PE stained for CD4/cd8. I found some papers that look impressive. **************************** I presume you mean the Beckstead zinc TRIS fixative that is formalin free? It should work with murine or rat tissues fixed in ZnTRIS since the tissues can then be paraffin processed, but avoid any solvent that have NBF carry over so these antigens are not compromised by that sniiff of aldehyde. The fixative can be purchased from BD Bioscience/Invitrogen. However, PE (phycoerythrin) fades rapidly before your eyes while you are looking at the stained sections. Been there, and seen it happen resulting in a lot of wasted time and effort. PE is for FACS not fluorescence microscopy. Not only that, but doing direct immunofluorescence using CD4 or CD8 conjugated to a fluorophore will result in poor to no staining since the fluorphore molecules in close approximation to each other will exchange electrons and quench fluorescence. It is better to do CD4 or CD8 then come back with an excellent secondary e.g. Jacksons donkey anti mouse F(ab')2 frag of IgG conjugated to Alexa 594 OR use a biotinylated CD4 and CD8 and come back with Streptavidin Alexa 594. Double staining using this fixative should be very possible, even triple IF. I have several publications for this fixative including the original Beckstead (for human) and Nitta et al (for murine) CD marker publications. We tried ZnTris for chromogenic IHC with success but didn't continue to use it since we did the work much faster with frozen sections. We found this fixative did not work well on fresh tissue frozen sections though, and preferred to use our favorite 75% acetone/25% absolute ethanol for 5 minutes at RT on air dried frozen sections then into buffer from this solvent fixative (sections were not alllowed to air dry again after this fixaton). As for papers you have seen, can you give the references please? Gayle Callis HTL/HT/MT(ASCP) From tony.henwood <@t> health.nsw.gov.au Thu Feb 14 17:30:48 2013 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Thu Feb 14 17:31:11 2013 Subject: [Histonet] standard protocol In-Reply-To: <8CFD8E3CEBD8C39-130C-23C72@webmail-d142.sysops.aol.com> References: <8CFD8E3CEBD8C39-130C-23C72@webmail-d142.sysops.aol.com> Message-ID: <6D6BD1DE8A5571489398B392A38A71579D231C0E@xmdb04.nch.kids> Hi Kay, Who was the idiot who sent this to you? Why bother cutting the sections if they will float off in the xylene, you might as well just send them blank slides. The companies are willing to spend millions of dollars on the development but couldn't be bothered seeking professional advice before they send out mandatory protocols like this. Unfortunately I regularly see this and usually send back a letter stating that to follow their protocol we will be charging more for the stupidity. I do not tell them what they should be doing unless they are willing to pay for my advice. You can forward my reply on to them if you so wish. Here ends my Friday rant!! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Histopatty Sent: Friday, 15 February 2013 3:02 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] standard protocol We are getting instructions from a pharmaceutical company who needs slides, with instructions stating "sections should be floated per standard protocol, xylene dipped from 3 minutes and dried for 10 minutes." I'm reaching out to those of you who routinly deal with research protocols and wondering is the "standard protocol" the normal float, dri, melt? Patricia Eneff for Kay Pierce Please respond to sharon.pierce@hcahealthcare.com if possilbe. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From tony.henwood <@t> health.nsw.gov.au Thu Feb 14 17:35:18 2013 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Thu Feb 14 17:35:32 2013 Subject: [Histonet] RE: "Vacuole" appearance in lymph Nodes In-Reply-To: <3855827CD3E36249A30D57F6F896F8F17CC022A7@EXMBX2.aruplab.net> References: <3855827CD3E36249A30D57F6F896F8F17CC022A7@EXMBX2.aruplab.net> Message-ID: <6D6BD1DE8A5571489398B392A38A71579D231C2C@xmdb04.nch.kids> Ken, Are the Microtomists being over enthusiastic with the block "trimming" or "facing"? This is more prone to happen if the block is cold (ie harder) during trimming. I would recommend after trimming to full-face that a few slower turns of the microtome wheel at 4-5 microns will clear the holes (if this is indeed the cause of the holes) Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Metzger, Kenneth Sent: Friday, 15 February 2013 3:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] "Vacuole" appearance in lymph Nodes Hi All, I have sent this question to respected colleagues individually so pardon my redundancy if you have this email already. Lately we have had random nodes show up with areas that appear to have multiple vacuoles through-out the specimen. They have different patterns section to section so I know it is not in the tissue. The staining looks fine and the tissue cuts well so I don't think it is a processing issue. Any ideas on the problem would be welcome. Thanks Ken Kenneth G Metzger HTL(ASCP) Histology Supervisor ARUP Labs Salt Lake City, Utah Phone: (801)583-2787 ext. 3101 Fax: (801) 584-5244 Email: kenneth.metzger@aruplab.com ------------------------------------------------------------------- The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws, or protected health information and is intended only for the use of the recipient. Unauthorized forwarding, printing, copying, distributing, or use of such information is strictly prohibited and may be unlawful. If you are not the intended recipient, please promptly delete this e-mail and notify the sender of the delivery error or you may call ARUP Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 (800) 522-2787 ext. 2100 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From b427297 <@t> aol.com Thu Feb 14 17:42:32 2013 From: b427297 <@t> aol.com (Jackie O'Connor) Date: Thu Feb 14 17:42:38 2013 Subject: [Histonet] Chromic Acid for Cleaning Glass In-Reply-To: <457439$l9rv1@correo2bi.usc.es> References: <457439$l9rv1@correo2bi.usc.es> Message-ID: <8CFD9241776B9CF-CEC-2C3AF@Webmail-m120.sysops.aol.com> Household bleach is a reasonable alternative for acid cleaning glassware. Jackie -----Original Message----- From: Pablo Sanchez-Quinteiro To: Histonet Sent: Thu, Feb 14, 2013 3:37 am Subject: [Histonet] Chromic Acid for Cleaning Glass Dear Listers, I think that the use of Chromic Acid for cleaning the glassware is currently declined. Do you keep using it? Could you suggest me a non-comercial alternative? Thanks in advance, Pablo (Spain) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From max_histo_00 <@t> yahoo.it Fri Feb 15 01:49:40 2013 From: max_histo_00 <@t> yahoo.it (Massimo) Date: Fri Feb 15 01:49:48 2013 Subject: [Histonet] Chromic Acid for Cleaning Glass In-Reply-To: <457439$l9rv1@correo2bi.usc.es> References: <457439$l9rv1@correo2bi.usc.es> Message-ID: <1360914580.18105.YahooMailNeo@web172004.mail.ir2.yahoo.com> I use to clean the glassware with the usual household detergents, then washing them carefully with distilled water. I pay attention on degreasing the slides and the cover glasses. Greasy traces are responsible of the?detachment of the specimen slices attached on them. I use to clean them with thin powder of?calcium carbonate (CaCO3).? I put the slide on a glass plate then with a finger covered with a clean cloth I rub the surface where I have put a small quantity of powder. The same on the other side of the slide. Then I wash with tap water and finally?with distilled water. Then I dry only the side of the slide where I'll attach the specimen. Massimo Tosi "A humble Chemical Engineer who loves Histology" ________________________________ Da: Pablo Sanchez-Quinteiro A: Histonet@lists.utsouthwestern.edu Inviato: Gioved? 14 Febbraio 2013 10:36 Oggetto: [Histonet] Chromic Acid for Cleaning Glass Dear Listers, I think that the use of Chromic Acid for cleaning the glassware is currently declined. Do you keep using it? Could you suggest me a non-comercial alternative? Thanks in advance, Pablo (Spain) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From max_histo_00 <@t> yahoo.it Fri Feb 15 02:30:27 2013 From: max_histo_00 <@t> yahoo.it (Massimo) Date: Fri Feb 15 04:58:08 2013 Subject: [Histonet] Chromic Acid for Cleaning Glass In-Reply-To: <457439$l9rv1@correo2bi.usc.es> References: <457439$l9rv1@correo2bi.usc.es> Message-ID: <1360917027.48140.YahooMailNeo@web172002.mail.ir2.yahoo.com> I forgot ... It's easy to understand when a slide glass is greasy free. It's enough to put on it a drop of distilled water. If it spreads on the glass it's OK. Usually the slides are coming already clean from the factory, but I don't trust in that. There is nothing more depressing as to see a slice to detach from a slide through?a process. Massimo Tosi "A humble Chemical Engineer who loves Histology" ________________________________ Da: Pablo Sanchez-Quinteiro A: Histonet@lists.utsouthwestern.edu Inviato: Gioved? 14 Febbraio 2013 10:36 Oggetto: [Histonet] Chromic Acid for Cleaning Glass Dear Listers, I think that the use of Chromic Acid for cleaning the glassware is currently declined. Do you keep using it? Could you suggest me a non-comercial alternative? Thanks in advance, Pablo (Spain) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Nancy_Schmitt <@t> pa-ucl.com Fri Feb 15 06:19:38 2013 From: Nancy_Schmitt <@t> pa-ucl.com (Nancy Schmitt) Date: Fri Feb 15 18:54:55 2013 Subject: [Histonet] Type I Water Message-ID: <906B4DA90ED1DB4DB6C7E94D7CEE6C364DC6EDDE@PEITHA.wad.pa-ucl.com> Good Morning- I feel like I am racking up frequent flier miles on Histonet lately:) We are seeing bacteria in our type I. This requires us to obtain our Type I from another of our lab sites. Do I have any other options? Like tap water? Nancy Schmitt HT, MLT(ASCP) NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From Nancy_Schmitt <@t> pa-ucl.com Fri Feb 15 08:26:04 2013 From: Nancy_Schmitt <@t> pa-ucl.com (Nancy Schmitt) Date: Fri Feb 15 19:20:55 2013 Subject: [Histonet] Type I Water Message-ID: <906B4DA90ED1DB4DB6C7E94D7CEE6C364DC6EE22@PEITHA.wad.pa-ucl.com> Clearly I sent this too quickly (early) - this is in regards to the water in our tissue float baths - does it HAVE to be Type I? Thanks Nancy Good Morning- I feel like I am racking up frequent flier miles on Histonet lately:) We are seeing bacteria in our type I. This requires us to obtain our Type I from another of our lab sites. Do I have any other options? Like tap water? Nancy Schmitt HT, MLT(ASCP) NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From sdysart <@t> mirnarx.com Fri Feb 15 08:38:13 2013 From: sdysart <@t> mirnarx.com (Sarah Dysart) Date: Fri Feb 15 19:25:53 2013 Subject: [Histonet] RE: Type I Water In-Reply-To: <906B4DA90ED1DB4DB6C7E94D7CEE6C364DC6EE22@PEITHA.wad.pa-ucl.com> References: <906B4DA90ED1DB4DB6C7E94D7CEE6C364DC6EE22@PEITHA.wad.pa-ucl.com> Message-ID: <8A70A9B2ECDD084DACFE6C59FCF86D5048504D23@BL2PRD0711MB434.namprd07.prod.outlook.com> I put tap water in my water bath and haven't had issues. Alternatively you could use DI water which would be a little cleaner... Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt Sent: Friday, February 15, 2013 8:26 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Type I Water Clearly I sent this too quickly (early) - this is in regards to the water in our tissue float baths - does it HAVE to be Type I? Thanks Nancy Good Morning- I feel like I am racking up frequent flier miles on Histonet lately:) We are seeing bacteria in our type I. This requires us to obtain our Type I from another of our lab sites. Do I have any other options? Like tap water? Nancy Schmitt HT, MLT(ASCP) NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek <@t> digestivespecialists.com Fri Feb 15 08:43:27 2013 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Fri Feb 15 19:25:56 2013 Subject: [Histonet] RE: Type I Water In-Reply-To: <906B4DA90ED1DB4DB6C7E94D7CEE6C364DC6EE22@PEITHA.wad.pa-ucl.com> References: <906B4DA90ED1DB4DB6C7E94D7CEE6C364DC6EE22@PEITHA.wad.pa-ucl.com> Message-ID: <5A2BD13465E061429D6455C8D6B40E39164CEBFCE1@IBMB7Exchange.digestivespecialists.com> I have used tap water without any problems. The only thing is you need to be sure you clean your waterbath well at the end of the day and dry it out. What I experienced was hard water residue on the waterbath. Linda -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt Sent: Friday, February 15, 2013 9:26 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Type I Water Clearly I sent this too quickly (early) - this is in regards to the water in our tissue float baths - does it HAVE to be Type I? Thanks Nancy Good Morning- I feel like I am racking up frequent flier miles on Histonet lately:) We are seeing bacteria in our type I. This requires us to obtain our Type I from another of our lab sites. Do I have any other options? Like tap water? Nancy Schmitt HT, MLT(ASCP) NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akemiat3377 <@t> yahoo.com Fri Feb 15 08:57:08 2013 From: akemiat3377 <@t> yahoo.com (Akemi Allison) Date: Fri Feb 15 19:27:30 2013 Subject: [Histonet] RE: Type I Water In-Reply-To: <8A70A9B2ECDD084DACFE6C59FCF86D5048504D23@BL2PRD0711MB434.namprd07.prod.outlook.com> References: <906B4DA90ED1DB4DB6C7E94D7CEE6C364DC6EE22@PEITHA.wad.pa-ucl.com> <8A70A9B2ECDD084DACFE6C59FCF86D5048504D23@BL2PRD0711MB434.namprd07.prod.outlook.com> Message-ID: <1360940228.81721.YahooMailNeo@web140603.mail.bf1.yahoo.com> It is recommended to use DI water for your water bath, especially when cutting tissues for IHC. ?Tap water has all kinds of bacteria, as well as potential chorine, rust, lead and other components which might have adverse affects to your histologic testing. ?Ethel Macrea from Ventana has presented an excellent workshop on water for use in the histology lab at NSH national and state meetings. ?If you have an opportunity i highly recommend attending ?As a good housekeeping practice, I always recommend cleaning your water baths with a soapy detergent after use, rinsing with tap water, drying residual water from the water bath and either turning upside down, or covering to prevent dust particles. ? Akemi Allison BS, HT(ASCP)HTL ________________________________ From: Sarah Dysart To: Nancy Schmitt ; "histonet@lists.utsouthwestern.edu" Sent: Friday, February 15, 2013 6:38 AM Subject: [Histonet] RE: Type I Water I put tap water in my water bath and haven't had issues.? Alternatively you could use DI water which would be a little cleaner... Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas? 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt Sent: Friday, February 15, 2013 8:26 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Type I Water Clearly I sent this too quickly (early) - this is in regards to the water in our tissue float baths - does it HAVE to be Type I? Thanks Nancy Good Morning- I feel like I am racking up frequent flier miles on Histonet lately:) We are seeing bacteria in our type I.? This requires us to obtain our Type I from another of our lab sites.? Do I have any other options?? Like tap water? Nancy Schmitt HT, MLT(ASCP) NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Fri Feb 15 09:20:13 2013 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Fri Feb 15 19:33:53 2013 Subject: AW: [Histonet] FISH baking and dewaxing In-Reply-To: References: Message-ID: <000901ce0b8f$f452a990$dcf7fcb0$@gmx.at> We see better results with FISH-slides baked over night. This is a phenomenon called aging, known from cytogenetics. It seems, that the time of air-drying and heat leads to a better access of the probes to the dna. Therefor we let them also in the oven over the weekend. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Houston, Ronald Gesendet: Donnerstag, 14. Februar 2013 20:08 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] FISH baking and dewaxing Can someone please explain why paraffin-embedded slides for FISH need to be baked for so long and have extensive dewaxing in xylenes and subsequent alcohol pretreatment? Our Molecular group bake for >1 hr at 65C, and then treat sections in 3 changes of xylene 15 min each, and 100% ethanol 15 minutes each x2 before air-drying. I have asked them why so long and get the standard response -- "that's the way we always do it"! Thanks Ronnie Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org "One person with passion is better than forty people merely interested." ~ E.M. Forster _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DJenkins <@t> bwmc.umms.org Fri Feb 15 09:25:18 2013 From: DJenkins <@t> bwmc.umms.org (Jenkins, Dalena) Date: Fri Feb 15 19:33:54 2013 Subject: [Histonet] Histology position Message-ID: <211C57CA9976E64AB2EFB3E7841DEE30033912D3@mail2.nah.org> Baltimore Washington Medical Center has an opening for a full time day-shift Histology technician. Hours are 8:30am - 5:00pm --- with Saturday rotation for embedding. (About every 6th week) Applicants must be HT registered or must meet the requirements to be eligible to sit for the HT certification exam. Must have a practical knowledge of the following: Accessioning Embedding Microtomy Frozen sectioning Special stains Preparation of solutions IHC Interested applicants can apply at www.mybwmc.org Or resumes can be forwarded to djenkins@bwmc.umms.org Dalena Jenkins, HT ASCP Supervisor Pathology Baltimore Washington Medical Center 301 Hospital Drive Glen Burnie, MD 21061 p. 410-787-4187 f. 410-595-1991 From BDeBrosse-Serra <@t> isisph.com Fri Feb 15 09:54:12 2013 From: BDeBrosse-Serra <@t> isisph.com (Bea DeBrosse-Serra) Date: Fri Feb 15 19:36:34 2013 Subject: [Histonet] standard protocol In-Reply-To: <6D6BD1DE8A5571489398B392A38A71579D231C0E@xmdb04.nch.kids> References: <8CFD8E3CEBD8C39-130C-23C72@webmail-d142.sysops.aol.com> <6D6BD1DE8A5571489398B392A38A71579D231C0E@xmdb04.nch.kids> Message-ID: <493CAA64F203E14E8823737B9EE0E25F092E5BA993@EXCHMB01.isis.local> I sure have never heard of a standard protocol like this with any pharmaceutical company I have ever worked for. This is disturbing! Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony Henwood (SCHN) Sent: Thursday, February 14, 2013 3:31 PM To: 'Histopatty'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] standard protocol Hi Kay, Who was the idiot who sent this to you? Why bother cutting the sections if they will float off in the xylene, you might as well just send them blank slides. The companies are willing to spend millions of dollars on the development but couldn't be bothered seeking professional advice before they send out mandatory protocols like this. Unfortunately I regularly see this and usually send back a letter stating that to follow their protocol we will be charging more for the stupidity. I do not tell them what they should be doing unless they are willing to pay for my advice. You can forward my reply on to them if you so wish. Here ends my Friday rant!! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Histopatty Sent: Friday, 15 February 2013 3:02 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] standard protocol We are getting instructions from a pharmaceutical company who needs slides, with instructions stating "sections should be floated per standard protocol, xylene dipped from 3 minutes and dried for 10 minutes." I'm reaching out to those of you who routinly deal with research protocols and wondering is the "standard protocol" the normal float, dri, melt? Patricia Eneff for Kay Pierce Please respond to sharon.pierce@hcahealthcare.com if possilbe. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Fri Feb 15 10:11:34 2013 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Fri Feb 15 19:38:39 2013 Subject: [Histonet] RE: Type I Water In-Reply-To: <906B4DA90ED1DB4DB6C7E94D7CEE6C364DC6EE22@PEITHA.wad.pa-ucl.com> References: <906B4DA90ED1DB4DB6C7E94D7CEE6C364DC6EE22@PEITHA.wad.pa-ucl.com> Message-ID: <761E2B5697F795489C8710BCC72141FF05568E@ex07.net.ucsf.edu> Nancy, You don't need Type 1 water for waterbaths. Type 1 (CLSI Reagent Grade) is for chemistry analytics - for which you want extremely pure water. For water-baths filtered tap water may be fine if it is filtered. For waterbaths you are mainly concerned about dirt, bacterial and sometimes mineral contaminants. Distilled water is probably the best since it is cleaner than tap water. We use type 1 for histochemistry stain applications but don't use very much so we buy it by the gallon from Thermo Fisher Scientific (cat# 9800-1). They call it NERL Reagent Grade Water. (NERL = NERL Diagnostic's, a Thermo Fisher company that makes medical-grade reagents. NERL used to mean New England Reagent Laboratory). Tim Morken UCSF Pathology -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt Sent: Friday, February 15, 2013 6:26 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Type I Water Clearly I sent this too quickly (early) - this is in regards to the water in our tissue float baths - does it HAVE to be Type I? Thanks Nancy Good Morning- I feel like I am racking up frequent flier miles on Histonet lately:) We are seeing bacteria in our type I. This requires us to obtain our Type I from another of our lab sites. Do I have any other options? Like tap water? Nancy Schmitt HT, MLT(ASCP) NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Fri Feb 15 10:20:45 2013 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Fri Feb 15 19:38:44 2013 Subject: [Histonet] standard protocol In-Reply-To: <493CAA64F203E14E8823737B9EE0E25F092E5BA993@EXCHMB01.isis.local> References: <8CFD8E3CEBD8C39-130C-23C72@webmail-d142.sysops.aol.com> <6D6BD1DE8A5571489398B392A38A71579D231C0E@xmdb04.nch.kids> <493CAA64F203E14E8823737B9EE0E25F092E5BA993@EXCHMB01.isis.local> Message-ID: <761E2B5697F795489C8710BCC72141FF0556A6@ex07.net.ucsf.edu> Sounds like a researcher passed on something they got from someone else, who got it 3rd hand. It is obviously incomplete so the best course is to contact the people in charge of the study and find out what they want (or THINK they want!). Ask them for the literature or actual protocol they are basing their request on so you can see it for yourself. Of course this is a common problem when dealing with researchers who know nothing about histology methods. Or maybe the request was written by an associate who was just copying boilerplate from some other study. They just need to be educated and my experience is that they are usually VERY grateful for the education. It helps them design studies better and eliminates a lot of headaches and future embarrassments when their studies fall behind or fail because they were ignorant of the process. Tim Morken UCSF Pathology -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Histopatty Sent: Friday, 15 February 2013 3:02 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] standard protocol We are getting instructions from a pharmaceutical company who needs slides, with instructions stating "sections should be floated per standard protocol, xylene dipped from 3 minutes and dried for 10 minutes." I'm reaching out to those of you who routinly deal with research protocols and wondering is the "standard protocol" the normal float, dri, melt? Patricia Eneff for Kay Pierce Please respond to sharon.pierce@hcahealthcare.com if possilbe. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From marktarango <@t> gmail.com Fri Feb 15 10:39:15 2013 From: marktarango <@t> gmail.com (Mark Tarango) Date: Fri Feb 15 19:43:45 2013 Subject: [Histonet] FISH baking and dewaxing In-Reply-To: <000901ce0b8f$f452a990$dcf7fcb0$@gmx.at> References: <000901ce0b8f$f452a990$dcf7fcb0$@gmx.at> Message-ID: The package insert for PathVysion (HER2 FISH) says to bake overnight at 56 degrees C. We never do this and our signals are clear, bright, and punctate. Many of the slides are just air-dried. Some are baked for about 20 minutes and I don't notice any difference between them. I wonder if I would see a difference with overnight baking... The aging step we use for cytology preps to be FISHed is 2 minutes in 2x SCC at 73 degrees C before protease digestion. I didn't know extended baking was considered an aging step for tissue sections to be FISHed. Thanks Gudrun! On Fri, Feb 15, 2013 at 7:20 AM, Gudrun Lang wrote: > We see better results with FISH-slides baked over night. This is a > phenomenon called aging, known from cytogenetics. > It seems, that the time of air-drying and heat leads to a better access of > the probes to the dna. > Therefor we let them also in the oven over the weekend. > Gudrun > > > -----Urspr?ngliche Nachricht----- > Von: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Houston, > Ronald > Gesendet: Donnerstag, 14. Februar 2013 20:08 > An: histonet@lists.utsouthwestern.edu > Betreff: [Histonet] FISH baking and dewaxing > > Can someone please explain why paraffin-embedded slides for FISH need to be > baked for so long and have extensive dewaxing in xylenes and subsequent > alcohol pretreatment? > Our Molecular group bake for >1 hr at 65C, and then treat sections in 3 > changes of xylene 15 min each, and 100% ethanol 15 minutes each x2 before > air-drying. I have asked them why so long and get the standard response -- > "that's the way we always do it"! > > Thanks > Ronnie > > Ronnie Houston, MS HT(ASCP)QIHC > Anatomic Pathology Manager > ChildLab, a Division of Nationwide Children's Hospital www.childlab.com > > 700 Children's Drive > Columbus, OH 43205 > (P) 614-722-5450 > (F) 614-722-2899 > ronald.houston@nationwidechildrens.org ronald.houston@nationwidechild > rens.org> > www.NationwideChildrens.org > > "One person with passion is better than forty people merely interested." > ~ E.M. Forster > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From BZIMMERM <@t> gru.edu Fri Feb 15 11:13:11 2013 From: BZIMMERM <@t> gru.edu (Zimmerman, Billie) Date: Fri Feb 15 19:47:16 2013 Subject: [Histonet] Georgia Society of Histotechnology Message-ID: <7B3DEB32E69C034EACB479059C5DE3FF755FBE@EX-MLB-03.ad.georgiahealth.edu> As we approach our upcoming celebration on Jekyll Island, GA., please take a few minutes to renew your membership. Go to our website at www.histosearch.com/gsh/ and renew online. Then, check out the educational opportunities that await you on one our fourteen barrier islands. With an "all-inclusive" price you can obtain CEU's through the NSH at an affordable price and in a resort setting. The all-inclusive price includes the following: opportunity to acquire up to 15 CEU's, vendor reception, breaks, continental breakfasts, awards luncheon, karaoke, and music in the bar area Friday and Saturday nights. It will give all of us the opportunity to network with fellow histotechs and notable speakers from our field. Please register, reserve your room, and get your nominations in for our 2013 awards. To nominate a deserving Histotech you must be a member in good standing and the nominee should be a member in good standing. If you have any questions, contact Wanda Simons or any of our officers and Board of Directors. We look forward to seeing you on Jekyll Island where...IT'S ALL GOOD". Sincerely, Wanda K. Simons HT(ASCP) GSH President Augusta State University and Georgia Health Sciences University have consolidated to become Georgia Regents University. Effective January 9, 2013, my email address has changed to BZIMMERM@gru.edu. Please update your address book to reflect this change. From vtobias <@t> uw.edu Fri Feb 15 12:34:36 2013 From: vtobias <@t> uw.edu (Victor A. Tobias) Date: Fri Feb 15 19:53:36 2013 Subject: [Histonet] Medical Antiques Estate Sale Message-ID: I came across this estate sale that has some pretty cool items. http://www.estatesales.net/estate-sales/382005.aspx Victor Tobias HT(ASCP) Clinical Applications Analyst Harborview Medical Center Dept of Pathology Room NJB244 Seattle, WA 98104 vtobias@u.washington.edu 206-744-2735 206-744-8240 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. From talulahgosh <@t> gmail.com Fri Feb 15 13:06:40 2013 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Fri Feb 15 19:55:42 2013 Subject: [Histonet] Medical Antiques Estate Sale In-Reply-To: References: Message-ID: Wow, that is AWESOME!!! Wish I were closer to TN to go!! Emily "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." -Chuck Palahniuk, "Haunted" On Fri, Feb 15, 2013 at 1:34 PM, Victor A. Tobias wrote: > I came across this estate sale that has some pretty cool items. > > http://www.estatesales.net/estate-sales/382005.aspx > > Victor Tobias HT(ASCP) > Clinical Applications Analyst > Harborview Medical Center > Dept of Pathology Room NJB244 > Seattle, WA 98104 > vtobias@u.washington.edu > 206-744-2735 > 206-744-8240 Fax > ================================================= > Privileged, confidential or patient identifiable information may be > contained in this message. This information is meant only for the use of > the intended recipients. If you are not the intended recipient, or if the > message has been addressed to you in error, do not read, disclose, > reproduce, distribute, disseminate or otherwise use this > transmission. Instead, please notify the sender by reply e-mail, and then > destroy all copies of the message and any attachments. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From JCBRITTON <@t> Cheshire-Med.COM Fri Feb 15 13:10:18 2013 From: JCBRITTON <@t> Cheshire-Med.COM (Britton, Josette C) Date: Fri Feb 15 19:55:44 2013 Subject: [Histonet] RE: "Vacuole" appearance in lymph Nodes In-Reply-To: <6D6BD1DE8A5571489398B392A38A71579D231C2C@xmdb04.nch.kids> Message-ID: Try soaking the face of the block after facing on a wet ice-cube tray! Josie Britton HT/QIHC(ASCP) Cheshire Medical Center Keene, NH 03431 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony Henwood (SCHN) Sent: Thursday, February 14, 2013 6:35 PM To: 'Metzger, Kenneth'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: "Vacuole" appearance in lymph Nodes Ken, Are the Microtomists being over enthusiastic with the block "trimming" or "facing"? This is more prone to happen if the block is cold (ie harder) during trimming. I would recommend after trimming to full-face that a few slower turns of the microtome wheel at 4-5 microns will clear the holes (if this is indeed the cause of the holes) Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Metzger, Kenneth Sent: Friday, 15 February 2013 3:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] "Vacuole" appearance in lymph Nodes Hi All, I have sent this question to respected colleagues individually so pardon my redundancy if you have this email already. Lately we have had random nodes show up with areas that appear to have multiple vacuoles through-out the specimen. They have different patterns section to section so I know it is not in the tissue. The staining looks fine and the tissue cuts well so I don't think it is a processing issue. Any ideas on the problem would be welcome. Thanks Ken Kenneth G Metzger HTL(ASCP) Histology Supervisor ARUP Labs Salt Lake City, Utah Phone: (801)583-2787 ext. 3101 Fax: (801) 584-5244 Email: kenneth.metzger@aruplab.com ------------------------------------------------------------------- The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws, or protected health information and is intended only for the use of the recipient. Unauthorized forwarding, printing, copying, distributing, or use of such information is strictly prohibited and may be unlawful. If you are not the intended recipient, please promptly delete this e-mail and notify the sender of the delivery error or you may call ARUP Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 (800) 522-2787 ext. 2100 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ************************************************************************ ********* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ************************************************************************ ********* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From contact <@t> excaliburpathology.com Fri Feb 15 13:45:54 2013 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Fri Feb 15 19:58:50 2013 Subject: [Histonet] Medical Antiques Estate Sale In-Reply-To: References: Message-ID: <1360957554.36486.YahooMailNeo@web5713.biz.mail.ne1.yahoo.com> Very cool, but also sad that the collection is being broken up. ? Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 8901 S. Santa Fe, Suite G Oklahoma City, OK 73139 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ________________________________ From: Victor A. Tobias To: "'histonet@lists.utsouthwestern.edu'" Sent: Friday, February 15, 2013 12:34 PM Subject: [Histonet] Medical Antiques Estate Sale I came across this estate sale that has some pretty cool items. http://www.estatesales.net/estate-sales/382005.aspx Victor Tobias HT(ASCP) Clinical Applications Analyst Harborview Medical Center Dept of Pathology Room NJB244 Seattle, WA 98104 vtobias@u.washington.edu 206-744-2735 206-744-8240 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mturner <@t> CSILaboratories.com Fri Feb 15 14:20:26 2013 From: mturner <@t> CSILaboratories.com (Mark Turner) Date: Fri Feb 15 20:02:52 2013 Subject: [Histonet] RE: Medical Antiques Estate Sale In-Reply-To: References: Message-ID: <643626B74DE2814D8537057F40E1A10B03CAA3FE@CSI-MX-NODEA.CSI-LABS.local> I'm going to be in Chattanooga tomorrow. I will try to get over there and see the collection. Thanks for the notice. Mark Turner, Ph.D., HT(ASCP)QIHC Manager, Histology/IHC ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victor A. Tobias Sent: Friday, February 15, 2013 1:35 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Medical Antiques Estate Sale I came across this estate sale that has some pretty cool items. http://www.estatesales.net/estate-sales/382005.aspx Victor Tobias HT(ASCP) Clinical Applications Analyst Harborview Medical Center Dept of Pathology Room NJB244 Seattle, WA 98104 vtobias@u.washington.edu 206-744-2735 206-744-8240 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Fri Feb 15 16:09:17 2013 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Fri Feb 15 20:19:41 2013 Subject: [Histonet] Benchmarking information In-Reply-To: <2A99E5980E5E0345B542E681A9A3F97804B52316@grouse.medcampus.org> References: <2A99E5980E5E0345B542E681A9A3F97804B52316@grouse.medcampus.org> Message-ID: The best option is your own process mapping and time studies of these processes working in your own lab environment. It is usually easiest if you intially break it down into the preanalyic, analytic and post analytic phases and the critical variables that reduce your delivery times or contribute to impact each of the bottlenecks/delays/errors/ problems, in each phasse, and then put these in problem statements in your LSS project charters. It gets convoluted if you put them all in one, so I think it is best if you find one that you can target as a "quick win" and use the momentum from this for further projects. Do a detailed "as is process" map from your current data and then work on streamlining by elimination of those items to build a more standardized and improved " how you want it to be" process map. This becomes your SOP more or less for the new procedure. I know you wanted numbers, but I guess I feel your own numbers are going to be a lot more useful. However, there are a few references out there that are pretty detailed and are more or less case studies that have some numerical data. For example-The combined positive impact of Lean methodology and Ventana Symphony autostainer on histology lab workflow- http://www.biomedcentral.com/1472-6890/10/2 This article may give you some ideas to get started. Joelle Weaver MAOM, HTL (ASCP) QIHC > Date: Wed, 13 Feb 2013 11:45:27 -0600 > From: Sheila.Tapper@essentiahealth.org > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Benchmarking information > > We are working on a Lean/SixSigma project in our Histology Lab to > decrease our TAT for pathology reports. Part of this process is to > break down the total time and look at the different stages of the > process to help identify where waste in the process is, such as: > > * Time from receipt in lab to delivery of slides to pathologists > * Time special stains are ordered to delivery of slides to > pathologists > * Etc. > > Does anyone have any benchmarking information out there to share on > this? Any help is appreciated. > > > > _________________________________________________ > > Sheila Tapper > Anatomic Pathology Supervisor > Essentia Health > SMDC Laboratory > Pathology 3W SMMC > 407 East Third Street, Duluth, MN 55805 > P: 218-786-5472 | F: 218-786-2369 > > sheila.tapper@essentiahealth.org > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From irena.kirbis <@t> hotmail.com Sat Feb 16 08:58:48 2013 From: irena.kirbis <@t> hotmail.com (IRENA SREBOTNIK KIRBIS) Date: Sat Feb 16 08:58:50 2013 Subject: [Histonet] FISH signals Message-ID: Hi, how the FISH signals in FFPE tissue can be strenghten? by standard procedure only weak signals for EGFR can be obtained, so far I tried different time in Vysis protease 1 (45, 60, 70 min), for other probes 70 min works quite well on our FFPE, but not for EGFR? what other modifications can I try? many thanks Irena From rjbuesa <@t> yahoo.com Sat Feb 16 09:54:38 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Feb 16 09:54:44 2013 Subject: [Histonet] Benchmarking information In-Reply-To: References: <2A99E5980E5E0345B542E681A9A3F97804B52316@grouse.medcampus.org> Message-ID: <1361030078.66603.YahooMailNeo@web163104.mail.bf1.yahoo.com> I feel the "urge" of commenting on this advise about how to determine a benchmark for a histology lab. ? 1- I agree that you should first know your lab and how long it takes for your techs to complete the work-flow from receiving the specimens to reporting the final diagnosis BUT 2- if you just stop at that point you will be unable to determine if you have a problem. Finding problems and developing solutions can only be achieved if you COMPARE your lab to others and how efficient you are, how your TAT in each step of the process compares with with other labs of the same size (similar workload, staff and technology). 3- unless you compare you cannot develop strategies to do at least as well as others do. 4- to achieve the comparison objective you have to have information about other labs and you can only find about that if?you hire a consultant (very expensive) or if you reffer yourself to published information. I think that you will find useful some articles I have written on the subject and that you can find at http://www.histosearch.com/rene.html ? Finally, the article you have been referred to (http://www.biomedcentral.com/1472-6890/10/2) in its original version was a propaganda for the VentanaSymphony autostainer and had to be rewritten to achieve its present form. Essentially lacks the information you need (no offense intended, just the facts from my point of view). Ren? J. ? From: joelle weaver To: sheila.tapper@essentiahealth.org; histonet@lists.utsouthwestern.edu Sent: Friday, February 15, 2013 5:09 PM Subject: RE: [Histonet] Benchmarking information The best option is your own process mapping and time studies of these processes working in your own lab environment. It is usually easiest if you intially break it down into the preanalyic, analytic and post analytic phases and the critical variables that reduce your delivery times or contribute to impact each of the bottlenecks/delays/errors/ problems, in each phasse,? and then put these in problem statements in your? LSS project charters. It gets convoluted if you put them all in one, so I think it is best if you find one that you can target as a "quick win" and use the momentum from this for further projects. Do a detailed "as is process" map? from your current data and then work on streamlining by elimination of those items to build a more standardized and improved " how you want it to be" process map. This becomes your SOP more or less for the new procedure.? I know you wanted numbers, but I guess I feel your own numbers are going to be a lot more useful.? However, there are a few references out there that are pretty detailed and are more or less case studies that have some numerical data. For example-The combined positive impact of Lean methodology and Ventana Symphony autostainer on histology lab workflow-? http://www.biomedcentral.com/1472-6890/10/2 This article may give you some ideas to get started. Joelle Weaver MAOM, HTL (ASCP) QIHC > Date: Wed, 13 Feb 2013 11:45:27 -0600 > From: Sheila.Tapper@essentiahealth.org > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Benchmarking information > > We are working on a Lean/SixSigma project in our Histology Lab to > decrease our TAT for pathology reports.? Part of this process is to > break down the total time and look at the different stages of the > process to help identify where waste in the process is, such as: > > *??? Time from receipt in lab to delivery of slides to pathologists > *??? Time special stains are ordered to delivery of slides to > pathologists? > *??? Etc. > > Does anyone have any benchmarking information out there to share on > this?? Any help is appreciated.? > >? > > _________________________________________________ > > Sheila Tapper > Anatomic Pathology Supervisor > Essentia Health > SMDC Laboratory > Pathology 3W SMMC > 407 East Third Street, Duluth, MN 55805 > P: 218-786-5472 | F: 218-786-2369 > > sheila.tapper@essentiahealth.org > >? > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ??? ??? ??? ? ??? ??? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Sat Feb 16 15:27:06 2013 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Sat Feb 16 15:27:16 2013 Subject: [Histonet] Benchmarking information In-Reply-To: <1361030078.66603.YahooMailNeo@web163104.mail.bf1.yahoo.com> References: <2A99E5980E5E0345B542E681A9A3F97804B52316@grouse.medcampus.org> , <1361030078.66603.YahooMailNeo@web163104.mail.bf1.yahoo.com> Message-ID: it was just an example of process mapping and the like- the stainer is inconsequential really. There are a lot of options for ideas, just a quick suggestion for some graphics and numbers. Benchmarking is a start, to me that is all in improvement. I do not have any affiliation with ventana or any other vendor.But of course vendors are going to propagate information and articles that depict their products in the best light. As always just trying to add what my experience has been , if people dislike or disagree they can disregard Joelle Weaver MAOM, HTL (ASCP) QIHC Date: Sat, 16 Feb 2013 07:54:38 -0800 From: rjbuesa@yahoo.com Subject: Re: [Histonet] Benchmarking information To: joelleweaver@hotmail.com; sheila.tapper@essentiahealth.org; histonet@lists.utsouthwestern.edu I feel the "urge" of commenting on this advise about how to determine a benchmark for a histology lab. 1- I agree that you should first know your lab and how long it takes for your techs to complete the work-flow from receiving the specimens to reporting the final diagnosis BUT 2- if you just stop at that point you will be unable to determine if you have a problem. Finding problems and developing solutions can only be achieved if you COMPARE your lab to others and how efficient you are, how your TAT in each step of the process compares with with other labs of the same size (similar workload, staff and technology). 3- unless you compare you cannot develop strategies to do at least as well as others do. 4- to achieve the comparison objective you have to have information about other labs and you can only find about that if you hire a consultant (very expensive) or if you reffer yourself to published information. I think that you will find useful some articles I have written on the subject and that you can find at http://www.histosearch.com/rene.html Finally, the article you have been referred to (http://www.biomedcentral.com/1472-6890/10/2) in its original version was a propaganda for the VentanaSymphony autostainer and had to be rewritten to achieve its present form. Essentially lacks the information you need (no offense intended, just the facts from my point of view). Ren? J. From: joelle weaver To: sheila.tapper@essentiahealth.org; histonet@lists.utsouthwestern.edu Sent: Friday, February 15, 2013 5:09 PM Subject: RE: [Histonet] Benchmarking information The best option is your own process mapping and time studies of these processes working in your own lab environment. It is usually easiest if you intially break it down into the preanalyic, analytic and post analytic phases and the critical variables that reduce your delivery times or contribute to impact each of the bottlenecks/delays/errors/ problems, in each phasse, and then put these in problem statements in your LSS project charters. It gets convoluted if you put them all in one, so I think it is best if you find one that you can target as a "quick win" and use the momentum from this for further projects. Do a detailed "as is process" map from your current data and then work on streamlining by elimination of those items to build a more standardized and improved " how you want it to be" process map. This becomes your SOP more or less for the new procedure. I know you wanted numbers, but I guess I feel your own numbers are going to be a lot more useful. However, there are a few references out there that are pretty detailed and are more or less case studies that have some numerical data. For example-The combined positive impact of Lean methodology and Ventana Symphony autostainer on histology lab workflow- http://www.biomedcentral.com/1472-6890/10/2 This article may give you some ideas to get started. Joelle Weaver MAOM, HTL (ASCP) QIHC > Date: Wed, 13 Feb 2013 11:45:27 -0600 > From: Sheila.Tapper@essentiahealth.org > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Benchmarking information > > We are working on a Lean/SixSigma project in our Histology Lab to > decrease our TAT for pathology reports. Part of this process is to > break down the total time and look at the different stages of the > process to help identify where waste in the process is, such as: > > * Time from receipt in lab to delivery of slides to pathologists > * Time special stains are ordered to delivery of slides to > pathologists > * Etc. > > Does anyone have any benchmarking information out there to share on > this? Any help is appreciated. > > > > _________________________________________________ > > Sheila Tapper > Anatomic Pathology Supervisor > Essentia Health > SMDC Laboratory > Pathology 3W SMMC > 407 East Third Street, Duluth, MN 55805 > P: 218-786-5472 | F: 218-786-2369 > > sheila.tapper@essentiahealth.org > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Sun Feb 17 09:52:01 2013 From: joelleweaver <@t> hotmail.com (=?utf-8?B?am9lbGxld2VhdmVyQGhvdG1haWwuY29t?=) Date: Sun Feb 17 16:01:48 2013 Subject: =?utf-8?B?UmU6IFtIaXN0b25ldF0gQmVuY2htYXJraW5nIGluZm9ybWF0aW9u?= Message-ID: Sorry you did not care for my two cents, just trying to help Sent from my Verizon Wireless 4G LTE Smartphone ----- Reply message ----- From: "Rene J Buesa" To: "joelle weaver" , "sheila.tapper@essentiahealth.org" , "histonet@lists.utsouthwestern.edu" Subject: [Histonet] Benchmarking information Date: Sat, Feb 16, 2013 9:54 am I feel the "urge" of commenting on this advise about how to determine a benchmark for a histology lab. 1- I agree that you should first know your lab and how long it takes for your techs to complete the work-flow from receiving the specimens to reporting the final diagnosis BUT 2- if you just stop at that point you will be unable to determine if you have a problem. Finding problems and developing solutions can only be achieved if you COMPARE your lab to others and how efficient you are, how your TAT in each step of the process compares with with other labs of the same size (similar workload, staff and technology). 3- unless you compare you cannot develop strategies to do at least as well as others do. 4- to achieve the comparison objective you have to have information about other labs and you can only find about that if you hire a consultant (very expensive) or if you reffer yourself to published information. I think that you will find useful some articles I have written on the subject and that you can find at http://www.histosearch.com/rene.html Finally, the article you have been referred to (http://www.biomedcentral.com/1472-6890/10/2) in its original version was a propaganda for the VentanaSymphony autostainer and had to be rewritten to achieve its present form. Essentially lacks the information you need (no offense intended, just the facts from my point of view). Ren? J. From: joelle weaver To: sheila.tapper@essentiahealth.org; histonet@lists.utsouthwestern.edu Sent: Friday, February 15, 2013 5:09 PMSubject: RE: [Histonet] Benchmarking informationThe best option is your own process mapping and time studies of these processes working in your own lab environment. It is usually easiest if you intially break it down into the preanalyic, analytic and post analytic phases and the critical variables that reduce your delivery times or contribute to impact each of the bottlenecks/delays/errors/ problems, in each phasse, and then put these in problem statements in your LSS project charters. It gets convoluted if you put them all in one, so I think it is best if you find one that you can target as a "quick win" and use the momentum from this for further projects. Do a detailed "as is process" map from your current data and then work on streamlining by elimination of those items to build a more standardized and improved " how you want it to be" process map. This becomes your SOP more or less for the new procedure. I know you wanted numbers, but I guess I feel your own numbers are going to be a lot more useful. However, there are a few references out there that are pretty detailed and are more or less case studies that have some numerical data. For example-The combined positive impact of Lean methodology and Ventana Symphony autostainer on histology lab workflow- http://www.biomedcentral.com/1472-6890/10/2 This article may give you some ideas to get started. Joelle Weaver MAOM, HTL (ASCP) QIHC> Date: Wed, 13 Feb 2013 11:45:27 -0600> From: Sheila.Tapper@essentiahealth.org> To: histonet@lists.utsouthwestern.edu> Subject: [Histonet] Benchmarking information> > We are working on a Lean/SixSigma project in our Histology Lab to> decrease our TAT for pathology reports. Part of this process is to> break down the total time and look at the different stages of the> process to help identify where waste in the process is, such as:> > * Time from receipt in lab to delivery of slides to pathologists > * Time special stains are ordered to delivery of slides to> pathologists > * Etc. > > Does anyone have any benchmarking information out there to share on> this? Any help is appreciated. > > > > _________________________________________________> > Sheila Tapper> Anatomic Pathology Supervisor> Essentia Health> SMDC Laboratory> Pathology 3W SMMC> 407 East Third Street, Duluth, MN 55805> P: 218-786-5472 | F: 218-786-2369> > sheila.tapper@essentiahealth.org> > > > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________Histonet mailing listHistonet@lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Sun Feb 17 10:15:40 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sun Feb 17 16:34:59 2013 Subject: [Histonet] Benchmarking information In-Reply-To: References: Message-ID: <1361117740.89991.YahooMailNeo@web163102.mail.bf1.yahoo.com> Joel: It is not that I did not care about your response, it is that it was not a good advise. You have to remind that when somebody asks HistoNet it is because they do not know about the question and are seeking advise and very probably they will follow the advise. If somebody tries to improve the work flow of the lab and its TAT that person cannot look "inwards" because they will just understand?their operation and that does not guarantee improvement. The only way you have to improve is, after knowing what you do, is to compare and "emulate" those who have the best rates. Competition is the basis and you cannot compete if you only look "inwards". Your initial advise was correct (as I state) and allowed to know yourself but the improvement process has to expand to others. I also stated that I intended no offense so, if you felt offended, please forgive me. I was only trying to help a fellow HistoNeter! Ren? J. From: "joelleweaver@hotmail.com" To: Rene J Buesa ; "sheila.tapper@essentiahealth.org" ; "histonet@lists.utsouthwestern.edu" Sent: Sunday, February 17, 2013 10:52 AM Subject: Re: [Histonet] Benchmarking information Sorry you did not care for my two cents, just trying to help Sent from my Verizon Wireless 4G LTE Smartphone ----- Reply message ----- From: "Rene J Buesa" To: "joelle weaver" , "sheila.tapper@essentiahealth.org" , "histonet@lists.utsouthwestern.edu" Subject: [Histonet] Benchmarking information Date: Sat, Feb 16, 2013 9:54 am I feel the "urge" of commenting on this advise about how to determine a benchmark for a histology lab. ? 1- I agree that you should first know your lab and how long it takes for your techs to complete the work-flow from receiving the specimens to reporting the final diagnosis BUT 2- if you just stop at that point you will be unable to determine if you have a problem. Finding problems and developing solutions can only be achieved if you COMPARE your lab to others and how efficient you are, how your TAT in each step of the process compares with with other labs of the same size (similar workload, staff and technology). 3- unless you compare you cannot develop strategies to do at least as well as others do. 4- to achieve the comparison objective you have to have information about other labs and you can only find about that if?you hire a consultant (very expensive) or if you reffer yourself to published information. I think that you will find useful some articles I have written on the subject and that you can find at http://www.histosearch.com/rene.html ? Finally, the article you have been referred to (http://www.biomedcentral.com/1472-6890/10/2) in its original version was a propaganda for the VentanaSymphony autostainer and had to be rewritten to achieve its present form. Essentially lacks the information you need (no offense intended, just the facts from my point of view). Ren? J. ? From: joelle weaver To: sheila.tapper@essentiahealth.org; histonet@lists.utsouthwestern.edu Sent: Friday, February 15, 2013 5:09 PM Subject: RE: [Histonet] Benchmarking information The best option is your own process mapping and time studies of these processes working in your own lab environment. It is usually easiest if you intially break it down into the preanalyic, analytic and post analytic phases and the critical variables that reduce your delivery times or contribute to impact each of the bottlenecks/delays/errors/ problems, in each phasse,? and then put these in problem statements in your? LSS project charters. It gets convoluted if you put them all in one, so I think it is best if you find one that you can target as a "quick win" and use the momentum from this for further projects. Do a detailed "as is process" map? from your current data and then work on streamlining by elimination of those items to build a more standardized and improved " how you want it to be" process map. This becomes your SOP more or less for the new procedure.? I know you wanted numbers, but I guess I feel your own numbers are going to be a lot more useful.? However, there are a few references out there that are pretty detailed and are more or less case studies that have some numerical data. For example-The combined positive impact of Lean methodology and Ventana Symphony autostainer on histology lab workflow-? http://www.biomedcentral.com/1472-6890/10/2 This article may give you some ideas to get started. Joelle Weaver MAOM, HTL (ASCP) QIHC > Date: Wed, 13 Feb 2013 11:45:27 -0600 > From: Sheila.Tapper@essentiahealth.org > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Benchmarking information > > We are working on a Lean/SixSigma project in our Histology Lab to > decrease our TAT for pathology reports.? Part of this process is to > break down the total time and look at the different stages of the > process to help identify where waste in the process is, such as: > > *??? Time from receipt in lab to delivery of slides to pathologists > *??? Time special stains are ordered to delivery of slides to > pathologists? > *??? Etc. > > Does anyone have any benchmarking information out there to share on > this?? Any help is appreciated.? > >? > > _________________________________________________ > > Sheila Tapper > Anatomic Pathology Supervisor > Essentia Health > SMDC Laboratory > Pathology 3W SMMC > 407 East Third Street, Duluth, MN 55805 > P: 218-786-5472 | F: 218-786-2369 > > sheila.tapper@essentiahealth.org > >? > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ??? ??? ??? ? ??? ??? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vhlwong <@t> yahoo.com Sun Feb 17 20:34:12 2013 From: vhlwong <@t> yahoo.com (Victor Wong) Date: Mon Feb 18 05:38:39 2013 Subject: [Histonet] Safranin O staining Message-ID: <1361154852.62991.YahooMailNeo@web125202.mail.ne1.yahoo.com> Hi all, I am working on induced chondrogenesis in cell culture.? After, induction, I put the clump of cells (pellets) in 2% agarose to make a cell block.? When the pellet did not sink to the bottom of agarose, I heated to melt the agarose again and centrifuged with higher speed.?I fix the agar block in 10% formalin for an hour and then transfer to 70% alcohol and proceeded with normal paraffin procedure.? I stained the paraffin sections with Sigma Safranin O stain but got very faint or no staining.? Here is my protocol: 1. Weigert Iron Hx for 2 min 2. Acid alcohol,Scott Tap and wash 3. 0.02% fast green for 2 min 4. 0.1% acetic acid for 1 min 5. Sigma's Safranin O for 5-10 min 6. 95% alcohol then 3x 100% alcohol @3 min 7. xylene and mount I also tried to stain the sections with alcian blue pH1.0 and the staining was not quite remarkable.? I'd like to ask if repeated heating on embedded pellet affects staining as I wanted to make sure the pellet was at the bottom of agarose.? Are there other factors affect the staining. Any comments are welcomed. Thanks you in advance. Victor From Tony_Reilly <@t> health.qld.gov.au Sun Feb 17 23:50:25 2013 From: Tony_Reilly <@t> health.qld.gov.au (Tony Reilly) Date: Mon Feb 18 08:29:21 2013 Subject: [Histonet] Safranin O staining In-Reply-To: <1361154852.62991.YahooMailNeo@web125202.mail.ne1.yahoo.com> References: <1361154852.62991.YahooMailNeo@web125202.mail.ne1.yahoo.com> Message-ID: <51224DC1.411C.0039.0@health.qld.gov.au> Hi Victor I have been using agar cell blocks for over 30 years. It has been my experience that it is better to fix the cells in formalin prior to embedding in the agar to prevent damage to the cells from the heat of the agar. Another important step is to gently heat the agar so that it is just above melting point to also minimise the heat affect. If you are using microwaves to melt your agar do the following. -calibrate your microwave so that the temperature achieved is minimal -do not set and walk away, watch and stop heating as soon as melting is achieved -aliquot agar into small batches as the microwaves affect the agar such that the melting point increases with each use elevating the temperature of the agar. regards Tony Tony Reilly B.App.Sc. , M.Sc. Chief Scientist, Anatomical Pathology Pathology Queensland-PA Laboratory ________________________________________________ Health Services Support Agency | Department of Health Level 1, Building 15,Princess Alexandra Hospital Ipswich Road,WOOLLOONGABBA Qld4102 Ph: 07 3176 2412 Mob: 0402 139411 Fax: 07 3176 2930 Email: tony_reilly@health.qld.gov.au Web: www.health.qld.gov.au/qhcss/ >>> Victor Wong 2/18/2013 12:34 pm >>> Hi all, I am working on induced chondrogenesis in cell culture. After, induction, I put the clump of cells (pellets) in 2% agarose to make a cell block. When the pellet did not sink to the bottom of agarose, I heated to melt the agarose again and centrifuged with higher speed. I fix the agar block in 10% formalin for an hour and then transfer to 70% alcohol and proceeded with normal paraffin procedure. I stained the paraffin sections with Sigma Safranin O stain but got very faint or no staining. Here is my protocol: 1. Weigert Iron Hx for 2 min 2. Acid alcohol,Scott Tap and wash 3. 0.02% fast green for 2 min 4. 0.1% acetic acid for 1 min 5. Sigma's Safranin O for 5-10 min 6. 95% alcohol then 3x 100% alcohol @3 min 7. xylene and mount I also tried to stain the sections with alcian blue pH1.0 and the staining was not quite remarkable. I'd like to ask if repeated heating on embedded pellet affects staining as I wanted to make sure the pellet was at the bottom of agarose. Are there other factors affect the staining. Any comments are welcomed. Thanks you in advance. Victor _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ********************************************************************************** From vhlwong <@t> yahoo.com Mon Feb 18 01:09:36 2013 From: vhlwong <@t> yahoo.com (Victor Wong) Date: Mon Feb 18 09:26:46 2013 Subject: [Histonet] Safranin O staining In-Reply-To: <51224DC1.411C.0039.0@health.qld.gov.au> References: <1361154852.62991.YahooMailNeo@web125202.mail.ne1.yahoo.com> <51224DC1.411C.0039.0@health.qld.gov.au> Message-ID: <1361171376.10217.YahooMailNeo@web125202.mail.ne1.yahoo.com> Dear Tony, ? Thanks for your prompt reply. ? I fix the cells in 10% NBF for 1 hour, then changed to PBS and keep at 4C fridge.? To prepare cell block, I use a?heating water bath and?I usually need to set the heater to higher than 150C to liquidify the agarose (2% in PBS) without boiling.? The agar did not melt when heated at lower temperature.? I do think the agar is at less than hundred degree C but may be at 70C.? After then, I trimmed the block and fix again in formalin before putting in 70% alcohol.? Any comments? ? This is the first time I am working on cell block.? May I have your processing protocol? ? Best Regards, Victor ? From: Tony Reilly To: "histonet@lists.utsouthwestern.edu" ; Victor Wong Sent: Monday, February 18, 2013 1:50 PM Subject: Re: [Histonet] Safranin O staining Hi Victor I?have been using agar cell blocks for over 30 years.? It has been my experience that it is better to fix the cells in formalin prior to embedding in the agar to prevent damage to the cells from the heat of the agar.? Another important step is to gently heat the agar so that it is just above melting point to also minimise the heat affect.? If you are using microwaves to melt your agar do the following. -calibrate your microwave so that the temperature achieved is minimal -do not set and walk away, watch and stop heating as soon as melting is achieved -aliquot agar into small batches as the microwaves affect the agar such that the melting point increases with each use elevating the temperature of the agar. regards Tony Tony Reilly? B.App.Sc. ,?M.Sc. Chief Scientist, Anatomical Pathology Pathology Queensland-PA Laboratory ________________________________________________ Health Services Support Agency |?Department of?HealthLevel 1, Building 15,Princess Alexandra Hospital ? Ipswich Road,WOOLLOONGABBA? Qld4102 Ph: 07 3176 2412 Mob: 0402 139411 Fax: 07 3176 2930 Email: tony_reilly@health.qld.gov.au Web:??www.health.qld.gov.au/qhcss/ ? ? >>> Victor Wong 2/18/2013 12:34 pm >>> Hi all, I am working on induced chondrogenesis in cell culture.? After, induction, I put the clump of cells (pellets) in 2% agarose to make a cell block.? When the pellet did not sink to the bottom of agarose, I heated to melt the agarose again and centrifuged with higher speed. I fix the agar block in 10% formalin for an hour and then transfer to 70% alcohol and proceeded with normal paraffin procedure.? I stained the paraffin sections with Sigma Safranin O stain but got very faint or no staining.? Here is my protocol: 1. Weigert Iron Hx for 2 min 2. Acid alcohol,Scott Tap and wash 3. 0.02% fast green for 2 min 4. 0.1% acetic acid for 1 min 5. Sigma's Safranin O for 5-10 min 6. 95% alcohol then 3x 100% alcohol @3 min 7. xylene and mount I also tried to stain the sections with alcian blue pH1.0 and the staining was not quite remarkable.? I'd like to ask if repeated heating on embedded pellet affects staining as I wanted to make sure the pellet was at the bottom of agarose.? Are there other factors affect the staining. Any comments are welcomed. Thanks you in advance. Victor _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ********************************************************************************** From vhlwong <@t> yahoo.com Mon Feb 18 01:35:49 2013 From: vhlwong <@t> yahoo.com (Victor Wong) Date: Mon Feb 18 09:54:25 2013 Subject: [Histonet] Safranin O staining In-Reply-To: <6D6BD1DE8A5571489398B392A38A71579D23231C@xmdb04.nch.kids> References: <1361154852.62991.YahooMailNeo@web125202.mail.ne1.yahoo.com> <6D6BD1DE8A5571489398B392A38A71579D23231C@xmdb04.nch.kids> Message-ID: <1361172949.64418.YahooMailNeo@web125206.mail.ne1.yahoo.com> Dear Tony, ? Thank you for your prompt reply and the paper. ? I do fix the cell before processing in agarose and after embedding in agarose. ? It is inevitable to process in wax at higher than 45C as it is set by the routine workers. ? It is the first time I worked on cell block precessing.? Do you have protocol?of processing chondrogenic pellet by?tissue processor?? Any adjustment to my procesing and staining protocol?? ? I tried to stain the pH1.0 alcian blue for 45 minutes and overnght but unremarkable staining (faint staining) was observed.? Do you think glycosaminoglycans secreted by chondrogenic pellet can be destroyed by heat?? Best Regards, Victor From: Tony Henwood (SCHN) To: 'Victor Wong' ; "histonet@lists.utsouthwestern.edu" Sent: Monday, February 18, 2013 11:45 AM Subject: RE: [Histonet] Safranin O staining Victor, Heat can affect the way tissues and cells bind to dyes. I have attached an article that hints at some of these problems (Allison, R. T. and Bryant, D. (1998)'Effects of Processing at 45 C on Staining', Biotechnic and Histochemistry 73:3,128 -136). I have also found that some antigens are adversely affected by heat. CEA immunohistochemistry was decreased in cytology cell blocks made with molten agar compared to cell blocks made with fibrin clot. In both cases cell blocks were fixed in NBF after being made. Formalin seemed to protect the antigens in fibrin clots from subsequent heat damage during processing, whereas with agar cell blocks, the heat damage was already done. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victor Wong Sent: Monday, 18 February 2013 1:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Safranin O staining Hi all, I am working on induced chondrogenesis in cell culture.? After, induction, I put the clump of cells (pellets) in 2% agarose to make a cell block.? When the pellet did not sink to the bottom of agarose, I heated to melt the agarose again and centrifuged with higher speed.?I fix the agar block in 10% formalin for an hour and then transfer to 70% alcohol and proceeded with normal paraffin procedure.? I stained the paraffin sections with Sigma Safranin O stain but got very faint or no staining.? Here is my protocol: 1. Weigert Iron Hx for 2 min 2. Acid alcohol,Scott Tap and wash 3. 0.02% fast green for 2 min 4. 0.1% acetic acid for 1 min 5. Sigma's Safranin O for 5-10 min 6. 95% alcohol then 3x 100% alcohol @3 min 7. xylene and mount I also tried to stain the sections with alcian blue pH1.0 and the staining was not quite remarkable.? I'd like to ask if repeated heating on embedded pellet affects staining as I wanted to make sure the pellet was at the bottom of agarose.? Are there other factors affect the staining. Any comments are welcomed. Thanks you in advance. Victor _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From rjbuesa <@t> yahoo.com Mon Feb 18 06:42:11 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Feb 18 14:20:05 2013 Subject: [Histonet] Safranin O staining In-Reply-To: <1361154852.62991.YahooMailNeo@web125202.mail.ne1.yahoo.com> References: <1361154852.62991.YahooMailNeo@web125202.mail.ne1.yahoo.com> Message-ID: <1361191331.51979.YahooMailNeo@web163101.mail.bf1.yahoo.com> Believe it or not, not because you are dealing with cells (small as they are) they require longer than 1 hour to be correctly fixed. This is what I would do: fix the cells for 8 hours minimum ? prepare the pellet in agarose ? process to paraffin (FFPE) block ? section as usual. Now, check the distaining protocol, consult any histotechnique book because I think 2 min is Weigert is a very short time (remember the mordant) ? try different staining times, or follow a known protocol (you can find that in either Peter Gray's or Lillie's books). Ren? J. From: Victor Wong To: "histonet@lists.utsouthwestern.edu" Sent: Sunday, February 17, 2013 9:34 PM Subject: [Histonet] Safranin O staining Hi all, I am working on induced chondrogenesis in cell culture.? After, induction, I put the clump of cells (pellets) in 2% agarose to make a cell block.? When the pellet did not sink to the bottom of agarose, I heated to melt the agarose again and centrifuged with higher speed.?I fix the agar block in 10% formalin for an hour and then transfer to 70% alcohol and proceeded with normal paraffin procedure.? I stained the paraffin sections with Sigma Safranin O stain but got very faint or no staining.? Here is my protocol: 1. Weigert Iron Hx for 2 min 2. Acid alcohol,Scott Tap and wash 3. 0.02% fast green for 2 min 4. 0.1% acetic acid for 1 min 5. Sigma's Safranin O for 5-10 min 6. 95% alcohol then 3x 100% alcohol @3 min 7. xylene and mount I also tried to stain the sections with alcian blue pH1.0 and the staining was not quite remarkable.? I'd like to ask if repeated heating on embedded pellet affects staining as I wanted to make sure the pellet was at the bottom of agarose.? Are there other factors affect the staining. Any comments are welcomed. Thanks you in advance. Victor _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From koellingr <@t> comcast.net Mon Feb 18 08:42:06 2013 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Mon Feb 18 15:27:11 2013 Subject: [Histonet] Safranin O staining In-Reply-To: <1361154852.62991.YahooMailNeo@web125202.mail.ne1.yahoo.com> Message-ID: <1244923589.1045865.1361198526463.JavaMail.root@sz0001a.emeryville.ca.mail.comcast.net> Victor, I completely agree with Tony and Renee's methodology assessment but I would also ask you to look at your induced chondrogenesis model (I've never done this but have done many, many other cell culture?models).? If you go to this paper http://biotech.korea.ac.kr/bk21/bbs/upload/bk21bbs_cur_165_0.pdf ?in Tissue Engineering they describe in detail exactly what you are trying to do, if I read your question correctly.? Their pictures of safrinin 0 staining of pelleted cells from culture could be described as "light staining" if you compare them to the red/orange color what we all know safranin o looks like on articular surfaces.? I think that is a POOR positive control for their experiments but that's just me.? In-vitro cultured cells prepared for histology will seldom??stain the same as in-vivo material prepared for histology.? And they had to hit cells pretty hard, 5ng TGF to see some staining.? Have no idea what your cell culture methodologies are but?what I think you are doing?is all in that paper and you can see the results in the Safranin o pictures.? There are ways to get better, more similar, positive?staining controls then they used. ? Ray Koelling Research Scientist University of Washington Seattle ----- Original Message ----- From: "Victor Wong" To: histonet@lists.utsouthwestern.edu Sent: Sunday, February 17, 2013 6:34:12 PM Subject: [Histonet] Safranin O staining Hi all, I am working on induced chondrogenesis in cell culture.? After, induction, I put the clump of cells (pellets) in 2% agarose to make a cell block.? When the pellet did not sink to the bottom of agarose, I heated to melt the agarose again and centrifuged with higher speed.?I fix the agar block in 10% formalin for an hour and then transfer to 70% alcohol and proceeded with normal paraffin procedure.? I stained the paraffin sections with Sigma Safranin O stain but got very faint or no staining.? Here is my protocol: 1. Weigert Iron Hx for 2 min 2. Acid alcohol,Scott Tap and wash 3. 0.02% fast green for 2 min 4. 0.1% acetic acid for 1 min 5. Sigma's Safranin O for 5-10 min 6. 95% alcohol then 3x 100% alcohol @3 min 7. xylene and mount I also tried to stain the sections with alcian blue pH1.0 and the staining was not quite remarkable.? I'd like to ask if repeated heating on embedded pellet affects staining as I wanted to make sure the pellet was at the bottom of agarose.? Are there other factors affect the staining. Any comments are welcomed. Thanks you in advance. Victor _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Mon Feb 18 09:37:31 2013 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Mon Feb 18 15:36:37 2013 Subject: [Histonet] Safranin O staining In-Reply-To: <1361172949.64418.YahooMailNeo@web125206.mail.ne1.yahoo.com> References: <1361154852.62991.YahooMailNeo@web125202.mail.ne1.yahoo.com> <6D6BD1DE8A5571489398B392A38A71579D23231C@xmdb04.nch.kids>, <1361172949.64418.YahooMailNeo@web125206.mail.ne1.yahoo.com> Message-ID: <14E2C6176416974295479C64A11CB9AE016411AB470A@SBS2K8.premierlab.local> We have processed and stained these types of samples before. We do not embed the pellets in agarose prior to processing. We dye them with eosin and place them in a tea bag and process on a very short cycle - 10 minutes per station, embed section and stain. We have stained with safranin O, alcian blue, toluidine blue and IHC for collagen II. The safranin O comes out just fine as well as the others. We just receive the samples so I do not know anything about the preparation, collection and fixation. It possibly could be your construct, we have processed hundreds of these samples and the staining intensity and patterns do change. I expect this is due to the type of preparation. If you want to see pictures of the staining, you can go to this website: http://bioreagents.lifemapsc.com/products/purestem-chondropkg-4d20-8 or this paper: Sternberg H, Murai JT, Erickson IE, Funk WD, Das S, Wang Q, Snyder E, Chapman KB, Vangsness CT Jr, West MD. 2012 A human embryonic stem cell-derived clonal progenitor cell line with chondrogenic potential and markers of craniofacial mesenchyme. Regen Med. 7(4):481-501 Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 881-0763 cell (303) 682-9060 fax liz@premierlab.com Ship to address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victor Wong [vhlwong@yahoo.com] Sent: Monday, February 18, 2013 12:35 AM To: Tony Henwood (SCHN); histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Safranin O staining Dear Tony, Thank you for your prompt reply and the paper. I do fix the cell before processing in agarose and after embedding in agarose. It is inevitable to process in wax at higher than 45C as it is set by the routine workers. It is the first time I worked on cell block precessing. Do you have protocol of processing chondrogenic pellet by tissue processor? Any adjustment to my procesing and staining protocol? I tried to stain the pH1.0 alcian blue for 45 minutes and overnght but unremarkable staining (faint staining) was observed. Do you think glycosaminoglycans secreted by chondrogenic pellet can be destroyed by heat? Best Regards, Victor From: Tony Henwood (SCHN) To: 'Victor Wong' ; "histonet@lists.utsouthwestern.edu" Sent: Monday, February 18, 2013 11:45 AM Subject: RE: [Histonet] Safranin O staining Victor, Heat can affect the way tissues and cells bind to dyes. I have attached an article that hints at some of these problems (Allison, R. T. and Bryant, D. (1998)'Effects of Processing at 45 C on Staining', Biotechnic and Histochemistry 73:3,128 -136). I have also found that some antigens are adversely affected by heat. CEA immunohistochemistry was decreased in cytology cell blocks made with molten agar compared to cell blocks made with fibrin clot. In both cases cell blocks were fixed in NBF after being made. Formalin seemed to protect the antigens in fibrin clots from subsequent heat damage during processing, whereas with agar cell blocks, the heat damage was already done. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victor Wong Sent: Monday, 18 February 2013 1:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Safranin O staining Hi all, I am working on induced chondrogenesis in cell culture. After, induction, I put the clump of cells (pellets) in 2% agarose to make a cell block. When the pellet did not sink to the bottom of agarose, I heated to melt the agarose again and centrifuged with higher speed. I fix the agar block in 10% formalin for an hour and then transfer to 70% alcohol and proceeded with normal paraffin procedure. I stained the paraffin sections with Sigma Safranin O stain but got very faint or no staining. Here is my protocol: 1. Weigert Iron Hx for 2 min 2. Acid alcohol,Scott Tap and wash 3. 0.02% fast green for 2 min 4. 0.1% acetic acid for 1 min 5. Sigma's Safranin O for 5-10 min 6. 95% alcohol then 3x 100% alcohol @3 min 7. xylene and mount I also tried to stain the sections with alcian blue pH1.0 and the staining was not quite remarkable. I'd like to ask if repeated heating on embedded pellet affects staining as I wanted to make sure the pellet was at the bottom of agarose. Are there other factors affect the staining. Any comments are welcomed. Thanks you in advance. Victor _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histotalk <@t> yahoo.com Mon Feb 18 10:41:02 2013 From: histotalk <@t> yahoo.com (David Kemler) Date: Mon Feb 18 15:45:02 2013 Subject: [Histonet] Benchmarking information In-Reply-To: <1361117740.89991.YahooMailNeo@web163102.mail.bf1.yahoo.com> References: <1361117740.89991.YahooMailNeo@web163102.mail.bf1.yahoo.com> Message-ID: <1361205662.86429.YahooMailNeo@web121506.mail.ne1.yahoo.com> Way to?go Rene! Are you a politician on the side? LOL Just kidding - couldn't help myself! :) ? Yours, Dave ________________________________ From: Rene J Buesa To: "joelleweaver@hotmail.com" Cc: Histonet ; "Sheila.Tapper@essentiahealth.org" Sent: Sunday, February 17, 2013 11:15 AM Subject: Re: [Histonet] Benchmarking information Joel: It is not that I did not care about your response, it is that it was not a good advise. You have to remind that when somebody asks HistoNet it is because they do not know about the question and are seeking advise and very probably they will follow the advise. If somebody tries to improve the work flow of the lab and its TAT that person cannot look "inwards" because they will just understand?their operation and that does not guarantee improvement. The only way you have to improve is, after knowing what you do, is to compare and "emulate" those who have the best rates. Competition is the basis and you cannot compete if you only look "inwards". Your initial advise was correct (as I state) and allowed to know yourself but the improvement process has to expand to others. I also stated that I intended no offense so, if you felt offended, please forgive me. I was only trying to help a fellow HistoNeter! Ren? J. From: "joelleweaver@hotmail.com" To: Rene J Buesa ; "sheila.tapper@essentiahealth.org" ; "histonet@lists.utsouthwestern.edu" Sent: Sunday, February 17, 2013 10:52 AM Subject: Re: [Histonet] Benchmarking information Sorry you did not care for my two cents, just trying to help Sent from my Verizon Wireless 4G LTE Smartphone ----- Reply message ----- From: "Rene J Buesa" To: "joelle weaver" , "sheila.tapper@essentiahealth.org" , "histonet@lists.utsouthwestern.edu" Subject: [Histonet] Benchmarking information Date: Sat, Feb 16, 2013 9:54 am I feel the "urge" of commenting on this advise about how to determine a benchmark for a histology lab. ? 1- I agree that you should first know your lab and how long it takes for your techs to complete the work-flow from receiving the specimens to reporting the final diagnosis BUT 2- if you just stop at that point you will be unable to determine if you have a problem. Finding problems and developing solutions can only be achieved if you COMPARE your lab to others and how efficient you are, how your TAT in each step of the process compares with with other labs of the same size (similar workload, staff and technology). 3- unless you compare you cannot develop strategies to do at least as well as others do. 4- to achieve the comparison objective you have to have information about other labs and you can only find about that if?you hire a consultant (very expensive) or if you reffer yourself to published information. I think that you will find useful some articles I have written on the subject and that you can find at http://www.histosearch.com/rene.html ? Finally, the article you have been referred to (http://www.biomedcentral.com/1472-6890/10/2) in its original version was a propaganda for the VentanaSymphony autostainer and had to be rewritten to achieve its present form. Essentially lacks the information you need (no offense intended, just the facts from my point of view). Ren? J. ? From: joelle weaver To: sheila.tapper@essentiahealth.org; histonet@lists.utsouthwestern.edu Sent: Friday, February 15, 2013 5:09 PM Subject: RE: [Histonet] Benchmarking information The best option is your own process mapping and time studies of these processes working in your own lab environment. It is usually easiest if you intially break it down into the preanalyic, analytic and post analytic phases and the critical variables that reduce your delivery times or contribute to impact each of the bottlenecks/delays/errors/ problems, in each phasse,? and then put these in problem statements in your? LSS project charters. It gets convoluted if you put them all in one, so I think it is best if you find one that you can target as a "quick win" and use the momentum from this for further projects. Do a detailed "as is process" map? from your current data and then work on streamlining by elimination of those items to build a more standardized and improved " how you want it to be" process map. This becomes your SOP more or less for the new procedure.? I know you wanted numbers, but I guess I feel your own numbers are going to be a lot more useful.? However, there are a few references out there that are pretty detailed and are more or less case studies that have some numerical data. For example-The combined positive impact of Lean methodology and Ventana Symphony autostainer on histology lab workflow-? http://www.biomedcentral.com/1472-6890/10/2 This article may give you some ideas to get started. Joelle Weaver MAOM, HTL (ASCP) QIHC > Date: Wed, 13 Feb 2013 11:45:27 -0600 > From: Sheila.Tapper@essentiahealth.org > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Benchmarking information > > We are working on a Lean/SixSigma project in our Histology Lab to > decrease our TAT for pathology reports.? Part of this process is to > break down the total time and look at the different stages of the > process to help identify where waste in the process is, such as: > > *??? Time from receipt in lab to delivery of slides to pathologists > *??? Time special stains are ordered to delivery of slides to > pathologists? > *??? Etc. > > Does anyone have any benchmarking information out there to share on > this?? Any help is appreciated.? > >? > > _________________________________________________ > > Sheila Tapper > Anatomic Pathology Supervisor > Essentia Health > SMDC Laboratory > Pathology 3W SMMC > 407 East Third Street, Duluth, MN 55805 > P: 218-786-5472 | F: 218-786-2369 > > sheila.tapper@essentiahealth.org > >? > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ??? ??? ??? ? ??? ??? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Mon Feb 18 10:53:18 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Feb 18 15:45:05 2013 Subject: [Histonet] Benchmarking information In-Reply-To: <1361205662.86429.YahooMailNeo@web121506.mail.ne1.yahoo.com> References: <1361117740.89991.YahooMailNeo@web163102.mail.bf1.yahoo.com> <1361205662.86429.YahooMailNeo@web121506.mail.ne1.yahoo.com> Message-ID: <1361206398.18218.YahooMailNeo@web163101.mail.bf1.yahoo.com> No politician here, just plain old! Ren? J. From: David Kemler To: Rene J Buesa ; Fellow HistoNetters Sent: Monday, February 18, 2013 11:41 AM Subject: Re: [Histonet] Benchmarking information Way to?go Rene! Are you a politician on the side? LOL Just kidding - couldn't help myself! :) ? Yours, Dave From: Rene J Buesa To: "joelleweaver@hotmail.com" Cc: Histonet ; "Sheila.Tapper@essentiahealth.org" Sent: Sunday, February 17, 2013 11:15 AM Subject: Re: [Histonet] Benchmarking information Joel: It is not that I did not care about your response, it is that it was not a good advise. You have to remind that when somebody asks HistoNet it is because they do not know about the question and are seeking advise and very probably they will follow the advise. If somebody tries to improve the work flow of the lab and its TAT that person cannot look "inwards" because they will just understand?their operation and that does not guarantee improvement. The only way you have to improve is, after knowing what you do, is to compare and "emulate" those who have the best rates. Competition is the basis and you cannot compete if you only look "inwards". Your initial advise was correct (as I state) and allowed to know yourself but the improvement process has to expand to others. I also stated that I intended no offense so, if you felt offended, please forgive me. I was only trying to help a fellow HistoNeter! Ren? J. From: "joelleweaver@hotmail.com" To: Rene J Buesa ; "sheila.tapper@essentiahealth.org" ; "histonet@lists.utsouthwestern.edu" Sent: Sunday, February 17, 2013 10:52 AM Subject: Re: [Histonet] Benchmarking information Sorry you did not care for my two cents, just trying to help Sent from my Verizon Wireless 4G LTE Smartphone ----- Reply message ----- From: "Rene J Buesa" To: "joelle weaver" , "sheila.tapper@essentiahealth.org" , "histonet@lists.utsouthwestern.edu" Subject: [Histonet] Benchmarking information Date: Sat, Feb 16, 2013 9:54 am I feel the "urge" of commenting on this advise about how to determine a benchmark for a histology lab. ? 1- I agree that you should first know your lab and how long it takes for your techs to complete the work-flow from receiving the specimens to reporting the final diagnosis BUT 2- if you just stop at that point you will be unable to determine if you have a problem. Finding problems and developing solutions can only be achieved if you COMPARE your lab to others and how efficient you are, how your TAT in each step of the process compares with with other labs of the same size (similar workload, staff and technology). 3- unless you compare you cannot develop strategies to do at least as well as others do. 4- to achieve the comparison objective you have to have information about other labs and you can only find about that if?you hire a consultant (very expensive) or if you reffer yourself to published information. I think that you will find useful some articles I have written on the subject and that you can find at http://www.histosearch.com/rene.html ? Finally, the article you have been referred to (http://www.biomedcentral.com/1472-6890/10/2) in its original version was a propaganda for the VentanaSymphony autostainer and had to be rewritten to achieve its present form. Essentially lacks the information you need (no offense intended, just the facts from my point of view). Ren? J. ? From: joelle weaver To: sheila.tapper@essentiahealth.org; histonet@lists.utsouthwestern.edu Sent: Friday, February 15, 2013 5:09 PM Subject: RE: [Histonet] Benchmarking information The best option is your own process mapping and time studies of these processes working in your own lab environment. It is usually easiest if you intially break it down into the preanalyic, analytic and post analytic phases and the critical variables that reduce your delivery times or contribute to impact each of the bottlenecks/delays/errors/ problems, in each phasse,? and then put these in problem statements in your? LSS project charters. It gets convoluted if you put them all in one, so I think it is best if you find one that you can target as a "quick win" and use the momentum from this for further projects. Do a detailed "as is process" map? from your current data and then work on streamlining by elimination of those items to build a more standardized and improved " how you want it to be" process map. This becomes your SOP more or less for the new procedure.? I know you wanted numbers, but I guess I feel your own numbers are going to be a lot more useful.? However, there are a few references out there that are pretty detailed and are more or less case studies that have some numerical data. For example-The combined positive impact of Lean methodology and Ventana Symphony autostainer on histology lab workflow-? http://www.biomedcentral.com/1472-6890/10/2 This article may give you some ideas to get started. Joelle Weaver MAOM, HTL (ASCP) QIHC > Date: Wed, 13 Feb 2013 11:45:27 -0600 > From: Sheila.Tapper@essentiahealth.org > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Benchmarking information > > We are working on a Lean/SixSigma project in our Histology Lab to > decrease our TAT for pathology reports.? Part of this process is to > break down the total time and look at the different stages of the > process to help identify where waste in the process is, such as: > > *??? Time from receipt in lab to delivery of slides to pathologists > *??? Time special stains are ordered to delivery of slides to > pathologists? > *??? Etc. > > Does anyone have any benchmarking information out there to share on > this?? Any help is appreciated.? > >? > > _________________________________________________ > > Sheila Tapper > Anatomic Pathology Supervisor > Essentia Health > SMDC Laboratory > Pathology 3W SMMC > 407 East Third Street, Duluth, MN 55805 > P: 218-786-5472 | F: 218-786-2369 > > sheila.tapper@essentiahealth.org > >? > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ??? ??? ??? ? ??? ??? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tkngflght <@t> yahoo.com Mon Feb 18 12:25:07 2013 From: tkngflght <@t> yahoo.com (Cheryl) Date: Mon Feb 18 16:01:38 2013 Subject: [Histonet] slide and block discards Message-ID: <1361211907.28432.YahooMailClassic@web161903.mail.bf1.yahoo.com> Hello All- ? Does anyone have some authoritative document stating that histologic slides and/or blocks are non-infectious?? We all know this to be true--but is there a piece of paper that states this unequivocally? ? Thanks! Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab by helping one GREAT?Tech at a time.? 281.852.9457?Office 800.756.3309?Phone & Fax? admin@fullstaff.org ? From liz <@t> premierlab.com Mon Feb 18 12:32:32 2013 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Mon Feb 18 16:05:35 2013 Subject: [Histonet] slide and block discards In-Reply-To: <1361211907.28432.YahooMailClassic@web161903.mail.bf1.yahoo.com> References: <1361211907.28432.YahooMailClassic@web161903.mail.bf1.yahoo.com> Message-ID: <14E2C6176416974295479C64A11CB9AE016411AB470E@SBS2K8.premierlab.local> Just found this off the OSHA Website http://www.osha.gov/SLTC/bloodbornepathogens/recognition.html It lists how OSHA defines blood and it also lists the OPMI - other potentially infectious materials (unfixed tissue or organ) plus it has a bunch of guidance links on the topic. Hazard Recognition The CDC estimates that 5.6 million workers in the health care industry and related occupations are at risk of occupational exposure to bloodborne pathogens, including human immunodeficiency virus (HIV), hepatitis B virus (HBV), hepatitis C virus (HCV), and others. All occupational exposure to blood or other potentially infectious materials (OPIM) place workers at risk for infection with bloodborne pathogens. OSHA defines blood to mean human blood, human blood components, and products made from human blood. Other potentially infectious materials (OPIM) means: (1) The following human body fluids: semen, vaginal secretions, cerebrospinal fluid, synovial fluid, pleural fluid, pericardial fluid, peritoneal fluid, amniotic fluid, saliva in dental procedures, any body fluid that is visibly contaminated with blood, and all body fluids in situations where it is difficult or impossible to differentiate between body fluids; (2) Any unfixed tissue or organ (other than intact skin) from a human (living or dead); and (3) HIV-containing cell or tissue cultures, organ cultures, and HIV- or HBV-containing culture medium or other solutions; and blood, organs, or other tissues from experimental animals infected with HIV or HBV. The following references aid in recognizing workplace hazards associated with bloodborne pathogens. Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 881-0763 cell (303) 682-9060 fax liz@premierlab.com Ship to address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl [tkngflght@yahoo.com] Sent: Monday, February 18, 2013 11:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] slide and block discards Hello All- Does anyone have some authoritative document stating that histologic slides and/or blocks are non-infectious? We all know this to be true--but is there a piece of paper that states this unequivocally? Thanks! Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab by helping one GREAT Tech at a time. 281.852.9457 Office 800.756.3309 Phone & Fax admin@fullstaff.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CIngles <@t> uwhealth.org Mon Feb 18 12:39:41 2013 From: CIngles <@t> uwhealth.org (Ingles Claire) Date: Mon Feb 18 16:05:38 2013 Subject: [Histonet] slide and block discards In-Reply-To: <1361211907.28432.YahooMailClassic@web161903.mail.bf1.yahoo.com> References: <1361211907.28432.YahooMailClassic@web161903.mail.bf1.yahoo.com> Message-ID: I'd be interested in it too. Claire ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Cheryl [tkngflght@yahoo.com] Sent: Monday, February 18, 2013 12:25 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] slide and block discards Hello All- Does anyone have some authoritative document stating that histologic slides and/or blocks are non-infectious? We all know this to be true--but is there a piece of paper that states this unequivocally? Thanks! Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab by helping one GREAT Tech at a time. 281.852.9457 Office 800.756.3309 Phone & Fax admin@fullstaff.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Mon Feb 18 12:44:49 2013 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Mon Feb 18 16:05:40 2013 Subject: [Histonet] slides and blocks discards Message-ID: <14E2C6176416974295479C64A11CB9AE016411AB4711@SBS2K8.premierlab.local> I found this on the CDC website: http://www.cdc.gov/od/eaipp/faq.htm What type of material does NOT require an Etiologic Agent Import Permit? Non-infectious materials ? e.g., formalin-fixed specimens, tissues, or slides, or non-infectious human stem cells or non-infectious human organs for transplantation. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 881-0763 cell (303) 682-9060 fax liz@premierlab.com Ship to address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 From tkngflght <@t> yahoo.com Mon Feb 18 12:49:19 2013 From: tkngflght <@t> yahoo.com (Cheryl) Date: Mon Feb 18 16:07:12 2013 Subject: Fw: RE: [Histonet] slide and block discards Message-ID: <1361213359.81266.YahooMailClassic@web161906.mail.bf1.yahoo.com> ?Thanks to Liz for this CDC reference! --- On Mon, 2/18/13, Elizabeth Chlipala wrote: From: Elizabeth Chlipala Subject: RE: [Histonet] slide and block discards To: "Cheryl" Date: Monday, February 18, 2013, 10:40 AM #yiv640601932 P { MARGIN-TOP:0px;MARGIN-BOTTOM:0px;} This?is from the CDC ? http://www.cdc.gov/od/eaipp/faq.htm ? What type of material does NOT require an Etiologic Agent Import Permit? Non-infectious materials ? e.g., formalin-fixed specimens, tissues, or slides, or non-infectious human stem cells or non-infectious human organs for transplantation. ? Liz ? Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 881-0763 cell (303) 682-9060 fax liz@premierlab.com ? Ship to address: ? Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 From: Cheryl [tkngflght@yahoo.com] Sent: Monday, February 18, 2013 11:38 AM To: Elizabeth Chlipala Subject: RE: [Histonet] slide and block discards Nice.? So they clearly state UNFIXED but leave out FIXED tissue--which allows the assumption that fixed tissue is not on the list thus not biohazardous. ? Anything you've found that STATES this outright? ? I really want to close this loop!! ? Thank you! Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab by helping one GREAT?Tech at a time.? 281.852.9457?Office 800.756.3309?Phone & Fax? admin@fullstaff.org Sign up for the FREE?newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please?include your name and specialty in the body of the email. --- On Mon, 2/18/13, Elizabeth Chlipala wrote: From: Elizabeth Chlipala Subject: RE: [Histonet] slide and block discards To: "Cheryl" , "histonet@lists.utsouthwestern.edu" Date: Monday, February 18, 2013, 10:32 AM Just found this off the OSHA Website http://www.osha.gov/SLTC/bloodbornepathogens/recognition.html It lists how OSHA defines blood and it also lists the OPMI - other potentially infectious materials (unfixed tissue or organ) plus it has a bunch of guidance links on the topic. Hazard Recognition The CDC estimates that 5.6 million workers in the health care industry and related occupations are at risk of occupational exposure to bloodborne pathogens, including human immunodeficiency virus (HIV), hepatitis B virus (HBV), hepatitis C virus (HCV), and others. All occupational exposure to blood or other potentially infectious materials (OPIM) place workers at risk for infection with bloodborne pathogens. OSHA defines blood to mean human blood, human blood components, and products made from human blood. Other potentially infectious materials (OPIM) means: (1) The following human body fluids: semen, vaginal secretions, cerebrospinal fluid, synovial fluid, pleural fluid, pericardial fluid, peritoneal fluid, amniotic fluid, saliva in dental procedures, any body fluid that is visibly contaminated with blood, and all body fluids in situations where it is difficult or impossible to differentiate between body fluids; (2) Any unfixed tissue or organ (other than intact skin) from a human (living or dead); and (3) HIV-containing cell or tissue cultures, organ cultures, and HIV- or HBV-containing culture medium or other solutions; and blood, organs, or other tissues from experimental animals infected with HIV or HBV. The following references aid in recognizing workplace hazards associated with bloodborne pathogens. Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 881-0763 cell (303) 682-9060 fax liz@premierlab.com Ship to address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl [tkngflght@yahoo.com] Sent: Monday, February 18, 2013 11:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] slide and block discards Hello All- Does anyone have some authoritative document stating that histologic slides and/or blocks are non-infectious?? We all know this to be true--but is there a piece of paper that states this unequivocally? Thanks! Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab by helping one GREAT Tech at a time. 281.852.9457 Office 800.756.3309 Phone & Fax admin@fullstaff.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b-frederick <@t> northwestern.edu Mon Feb 18 13:07:00 2013 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Mon Feb 18 16:11:42 2013 Subject: [Histonet] RE: slides and blocks discards In-Reply-To: <14E2C6176416974295479C64A11CB9AE016411AB4711@SBS2K8.premierlab.local> References: <14E2C6176416974295479C64A11CB9AE016411AB4711@SBS2K8.premierlab.local> Message-ID: <62C639732D3F274DACED033EBDF6ADAF20D0358B@evcspmbx2.ads.northwestern.edu> Correct- we had to learn all this for IATA training (how to package and properly ship biological samples). There's a massive list. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala Sent: Monday, February 18, 2013 12:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] slides and blocks discards I found this on the CDC website: http://www.cdc.gov/od/eaipp/faq.htm What type of material does NOT require an Etiologic Agent Import Permit? Non-infectious materials - e.g., formalin-fixed specimens, tissues, or slides, or non-infectious human stem cells or non-infectious human organs for transplantation. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 881-0763 cell (303) 682-9060 fax liz@premierlab.com Ship to address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tony.henwood <@t> health.nsw.gov.au Mon Feb 18 16:44:27 2013 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Mon Feb 18 16:44:42 2013 Subject: [Histonet] Safranin O staining In-Reply-To: <1361172949.64418.YahooMailNeo@web125206.mail.ne1.yahoo.com> References: <1361154852.62991.YahooMailNeo@web125202.mail.ne1.yahoo.com> <6D6BD1DE8A5571489398B392A38A71579D23231C@xmdb04.nch.kids> <1361172949.64418.YahooMailNeo@web125206.mail.ne1.yahoo.com> Message-ID: <6D6BD1DE8A5571489398B392A38A71579D23253C@xmdb04.nch.kids> Hi Victor, I suppose the take home message from Russ's paper (God rest his soul - we do miss him) is that heat will affect some stains so extrapolating to routine processing temperatures, one could suppose that the deleterious effect would be more pronounced. To protect the cells, I would extend the fixation time and look at fixing before preparing the agar pellet. Or use the thrombin method instead (ask your Haematology department for expired thromboplastin from their clotting tests, use expired plasma from blood bank, add a few drops of 1% calcium chloride and a clot should form in 20seconds or so. Add NBF, fix for longer than an hour (4hr to overnight is better. And process as usual. Since the clots tend to be only about 3mm in greatest dimension (depending on the volume of thromboplastin and plasma used, a short gentle program will suffice (5 changes of alcohol, 20min each: 2 changes of xylene 30min each, 3 changes of wax, 30 min each). Alternatively you could mix the pellet with some egg albumin, add an equal volume of 10% formalin in alcohol, a nice clot will form, fix for a few hours and process from alcohol (Based on the paper, with modifications, by Yamamoto et al (1985) Am J Clin Pathol 83:409-414). Interstitial glycosaminoglycans and other mucins are also water soluble so fixing in formal alcohol should retain more of these "mucins" than aqueous NBF fixation (Nathan, & van Deth (1983) Pathology 15:301-4) Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA From: Victor Wong [mailto:vhlwong@yahoo.com] Sent: Monday, 18 February 2013 6:36 PM To: Tony Henwood (SCHN); histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Safranin O staining Dear Tony, Thank you for your prompt reply and the paper. I do fix the cell before processing in agarose and after embedding in agarose. It is inevitable to process in wax at higher than 45C as it is set by the routine workers. It is the first time I worked on cell block precessing. Do you have protocol of processing chondrogenic pellet by tissue processor? Any adjustment to my procesing and staining protocol? I tried to stain the pH1.0 alcian blue for 45 minutes and overnght but unremarkable staining (faint staining) was observed. Do you think glycosaminoglycans secreted by chondrogenic pellet can be destroyed by heat? Best Regards, Victor From: Tony Henwood (SCHN) To: 'Victor Wong' ; "histonet@lists.utsouthwestern.edu" Sent: Monday, February 18, 2013 11:45 AM Subject: RE: [Histonet] Safranin O staining Victor, Heat can affect the way tissues and cells bind to dyes. I have attached an article that hints at some of these problems (Allison, R. T. and Bryant, D. (1998)'Effects of Processing at 45 C on Staining', Biotechnic and Histochemistry 73:3,128 -136). I have also found that some antigens are adversely affected by heat. CEA immunohistochemistry was decreased in cytology cell blocks made with molten agar compared to cell blocks made with fibrin clot. In both cases cell blocks were fixed in NBF after being made. Formalin seemed to protect the antigens in fibrin clots from subsequent heat damage during processing, whereas with agar cell blocks, the heat damage was already done. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victor Wong Sent: Monday, 18 February 2013 1:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Safranin O staining Hi all, I am working on induced chondrogenesis in cell culture. After, induction, I put the clump of cells (pellets) in 2% agarose to make a cell block. When the pellet did not sink to the bottom of agarose, I heated to melt the agarose again and centrifuged with higher speed. I fix the agar block in 10% formalin for an hour and then transfer to 70% alcohol and proceeded with normal paraffin procedure. I stained the paraffin sections with Sigma Safranin O stain but got very faint or no staining. Here is my protocol: 1. Weigert Iron Hx for 2 min 2. Acid alcohol,Scott Tap and wash 3. 0.02% fast green for 2 min 4. 0.1% acetic acid for 1 min 5. Sigma's Safranin O for 5-10 min 6. 95% alcohol then 3x 100% alcohol @3 min 7. xylene and mount I also tried to stain the sections with alcian blue pH1.0 and the staining was not quite remarkable. I'd like to ask if repeated heating on embedded pellet affects staining as I wanted to make sure the pellet was at the bottom of agarose. Are there other factors affect the staining. Any comments are welcomed. Thanks you in advance. Victor _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From JMitchell <@t> uwhealth.org Mon Feb 18 18:03:16 2013 From: JMitchell <@t> uwhealth.org (Mitchell Jean A) Date: Mon Feb 18 18:03:23 2013 Subject: [Histonet] Safranin O staining In-Reply-To: <6D6BD1DE8A5571489398B392A38A71579D23253C@xmdb04.nch.kids> References: <1361154852.62991.YahooMailNeo@web125202.mail.ne1.yahoo.com> <6D6BD1DE8A5571489398B392A38A71579D23231C@xmdb04.nch.kids> <1361172949.64418.YahooMailNeo@web125206.mail.ne1.yahoo.com> <6D6BD1DE8A5571489398B392A38A71579D23253C@xmdb04.nch.kids> Message-ID: <16F90B93CA23D446980B3D591FD02DAD044644@UWHC-MBX14.uwhis.hosp.wisc.edu> I was just thinking about Russ Allison the other day. He is certainly missed. Jean Mitchell, BS HT (ASCP) University of Wisconsin Hospital & Clinics Neuromuscular Laboratory 600 Highland Avenue Madison, WI 53792-5132 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony Henwood (SCHN) Sent: Monday, February 18, 2013 4:44 PM To: 'Victor Wong'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Safranin O staining Hi Victor, I suppose the take home message from Russ's paper (God rest his soul - we do miss him) is that heat will affect some stains so extrapolating to routine processing temperatures, one could suppose that the deleterious effect would be more pronounced. To protect the cells, I would extend the fixation time and look at fixing before preparing the agar pellet. Or use the thrombin method instead (ask your Haematology department for expired thromboplastin from their clotting tests, use expired plasma from blood bank, add a few drops of 1% calcium chloride and a clot should form in 20seconds or so. Add NBF, fix for longer than an hour (4hr to overnight is better. And process as usual. Since the clots tend to be only about 3mm in greatest dimension (depending on the volume of thromboplastin and plasma used, a short gentle program will suffice (5 changes of alcohol, 20min each: 2 changes of xylene 30min each, 3 changes of wax, 30 min each). Alternatively you could mix the pellet with some egg albumin, add an equal volume of 10% formalin in alcohol, a nice clot will form, fix for a few hours and process from alcohol (Based on the paper, with modifications, by Yamamoto et al (1985) Am J Clin Pathol 83:409-414). Interstitial glycosaminoglycans and other mucins are also water soluble so fixing in formal alcohol should retain more of these "mucins" than aqueous NBF fixation (Nathan, & van Deth (1983) Pathology 15:301-4) Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA From: Victor Wong [mailto:vhlwong@yahoo.com] Sent: Monday, 18 February 2013 6:36 PM To: Tony Henwood (SCHN); histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Safranin O staining Dear Tony, Thank you for your prompt reply and the paper. I do fix the cell before processing in agarose and after embedding in agarose. It is inevitable to process in wax at higher than 45C as it is set by the routine workers. It is the first time I worked on cell block precessing. Do you have protocol of processing chondrogenic pellet by tissue processor? Any adjustment to my procesing and staining protocol? I tried to stain the pH1.0 alcian blue for 45 minutes and overnght but unremarkable staining (faint staining) was observed. Do you think glycosaminoglycans secreted by chondrogenic pellet can be destroyed by heat? Best Regards, Victor From: Tony Henwood (SCHN) To: 'Victor Wong' ; "histonet@lists.utsouthwestern.edu" Sent: Monday, February 18, 2013 11:45 AM Subject: RE: [Histonet] Safranin O staining Victor, Heat can affect the way tissues and cells bind to dyes. I have attached an article that hints at some of these problems (Allison, R. T. and Bryant, D. (1998)'Effects of Processing at 45 C on Staining', Biotechnic and Histochemistry 73:3,128 -136). I have also found that some antigens are adversely affected by heat. CEA immunohistochemistry was decreased in cytology cell blocks made with molten agar compared to cell blocks made with fibrin clot. In both cases cell blocks were fixed in NBF after being made. Formalin seemed to protect the antigens in fibrin clots from subsequent heat damage during processing, whereas with agar cell blocks, the heat damage was already done. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victor Wong Sent: Monday, 18 February 2013 1:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Safranin O staining Hi all, I am working on induced chondrogenesis in cell culture. After, induction, I put the clump of cells (pellets) in 2% agarose to make a cell block. When the pellet did not sink to the bottom of agarose, I heated to melt the agarose again and centrifuged with higher speed. I fix the agar block in 10% formalin for an hour and then transfer to 70% alcohol and proceeded with normal paraffin procedure. I stained the paraffin sections with Sigma Safranin O stain but got very faint or no staining. Here is my protocol: 1. Weigert Iron Hx for 2 min 2. Acid alcohol,Scott Tap and wash 3. 0.02% fast green for 2 min 4. 0.1% acetic acid for 1 min 5. Sigma's Safranin O for 5-10 min 6. 95% alcohol then 3x 100% alcohol @3 min 7. xylene and mount I also tried to stain the sections with alcian blue pH1.0 and the staining was not quite remarkable. I'd like to ask if repeated heating on embedded pellet affects staining as I wanted to make sure the pellet was at the bottom of agarose. Are there other factors affect the staining. Any comments are welcomed. Thanks you in advance. Victor _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vhlwong <@t> yahoo.com Mon Feb 18 21:24:10 2013 From: vhlwong <@t> yahoo.com (Victor Wong) Date: Mon Feb 18 21:25:40 2013 Subject: [Histonet] Safranin O staining In-Reply-To: <1361191331.51979.YahooMailNeo@web163101.mail.bf1.yahoo.com> References: <1361154852.62991.YahooMailNeo@web125202.mail.ne1.yahoo.com> <1361191331.51979.YahooMailNeo@web163101.mail.bf1.yahoo.com> Message-ID: <1361244250.71650.YahooMailNeo@web125204.mail.ne1.yahoo.com> Hi Rene, ? Thanks for your great suggestion. ? Yes, I'll extend the fixation time.? Do you think the embedding with melting agarose will be deletrious to the safranin staining?? I just use a heating water bath to melt the 2% agarose without temperature control.? I checked for complete melting and it was not boiled actually.?Can I go through routine automatic tissue processing instead of manually? ? For Weigert staining, we had a strong nuclear staining compared with faint or unstained red colour. ? Any enlightenment is welcomed. ? Best Regards, Victor ? From: Rene J Buesa To: Victor Wong ; "histonet@lists.utsouthwestern.edu" Sent: Monday, February 18, 2013 8:42 PM Subject: Re: [Histonet] Safranin O staining Believe it or not, not because you are dealing with cells (small as they are) they require longer than 1 hour to be correctly fixed. This is what I would do: fix the cells for 8 hours minimum ? prepare the pellet in agarose ? process to paraffin (FFPE) block ? section as usual. Now, check the distaining protocol, consult any histotechnique book because I think 2 min is Weigert is a very short time (remember the mordant) ? try different staining times, or follow a known protocol (you can find that in either Peter Gray's or Lillie's books). Ren? J. From: Victor Wong To: "histonet@lists.utsouthwestern.edu" Sent: Sunday, February 17, 2013 9:34 PM Subject: [Histonet] Safranin O staining Hi all, I am working on induced chondrogenesis in cell culture.? After, induction, I put the clump of cells (pellets) in 2% agarose to make a cell block.? When the pellet did not sink to the bottom of agarose, I heated to melt the agarose again and centrifuged with higher speed.?I fix the agar block in 10% formalin for an hour and then transfer to 70% alcohol and proceeded with normal paraffin procedure.? I stained the paraffin sections with Sigma Safranin O stain but got very faint or no staining.? Here is my protocol: 1. Weigert Iron Hx for 2 min 2. Acid alcohol,Scott Tap and wash 3. 0.02% fast green for 2 min 4. 0.1% acetic acid for 1 min 5. Sigma's Safranin O for 5-10 min 6. 95% alcohol then 3x 100% alcohol @3 min 7. xylene and mount I also tried to stain the sections with alcian blue pH1.0 and the staining was not quite remarkable.? I'd like to ask if repeated heating on embedded pellet affects staining as I wanted to make sure the pellet was at the bottom of agarose.? Are there other factors affect the staining. Any comments are welcomed. Thanks you in advance. Victor _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vhlwong <@t> yahoo.com Mon Feb 18 21:24:32 2013 From: vhlwong <@t> yahoo.com (Victor Wong) Date: Mon Feb 18 21:25:43 2013 Subject: [Histonet] Safranin O staining In-Reply-To: <1244923589.1045865.1361198526463.JavaMail.root@sz0001a.emeryville.ca.mail.comcast.net> References: <1361154852.62991.YahooMailNeo@web125202.mail.ne1.yahoo.com> <1244923589.1045865.1361198526463.JavaMail.root@sz0001a.emeryville.ca.mail.comcast.net> Message-ID: <1361244272.22394.YahooMailNeo@web125202.mail.ne1.yahoo.com> Hi Ray, ? Thank you for your suggestion and the paper.? There is a wonderful staining with safranin O compared to our staining that was very faint, nearly as unstained with safranin O (there was staining with fast green and hx). ? I dealed with sample as pellet in Falcon tubes, after experiment by my colleaque.? As far as I know, we isolated mesenchymal stem cells from marrow and cultivated in chondrogenic medium for days.? We don't have a pellet positive control but including a section with cartilage is useful. ? I really concerned that high temperature during melting the 2% agarose may be deletrious to the staining.? As suggested, I will extend the fixation time. ? Best Regards, Victor ? ? From: "koellingr@comcast.net" To: Victor Wong Cc: histonet@lists.utsouthwestern.edu Sent: Monday, February 18, 2013 10:42 PM Subject: Re: [Histonet] Safranin O staining Victor, I completely agree with Tony and Renee's methodology assessment but I would also ask you to look at your induced chondrogenesis model (I've never done this but have done many, many other cell culture?models).? If you go to this paper http://biotech.korea.ac.kr/bk21/bbs/upload/bk21bbs_cur_165_0.pdf?in Tissue Engineering they describe in detail exactly what you are trying to do, if I read your question correctly.? Their pictures of safrinin 0 staining of pelleted cells from culture could be described as "light staining" if you compare them to the red/orange color what we all know safranin o looks like on articular surfaces.? I think that is a POOR positive control for their experiments but that's just me.? In-vitro cultured cells prepared for histology will seldom??stain the same as in-vivo material prepared for histology.? And they had to hit cells pretty hard, 5ng TGF to see some staining.? Have no idea what your cell culture methodologies are but?what I think you are doing?is all in that paper and you can see the results in the Safranin o pictures.? There are ways to get better, more similar, positive?staining controls then they used. ? Ray Koelling Research Scientist University of Washington Seattle From: "Victor Wong" To: histonet@lists.utsouthwestern.edu Sent: Sunday, February 17, 2013 6:34:12 PM Subject: [Histonet] Safranin O staining Hi all, I am working on induced chondrogenesis in cell culture.? After, induction, I put the clump of cells (pellets) in 2% agarose to make a cell block.? When the pellet did not sink to the bottom of agarose, I heated to melt the agarose again and centrifuged with higher speed.?I fix the agar block in 10% formalin for an hour and then transfer to 70% alcohol and proceeded with normal paraffin procedure.? I stained the paraffin sections with Sigma Safranin O stain but got very faint or no staining.? Here is my protocol: 1. Weigert Iron Hx for 2 min 2. Acid alcohol,Scott Tap and wash 3. 0.02% fast green for 2 min 4. 0.1% acetic acid for 1 min 5. Sigma's Safranin O for 5-10 min 6. 95% alcohol then 3x 100% alcohol @3 min 7. xylene and mount I also tried to stain the sections with alcian blue pH1.0 and the staining was not quite remarkable.? I'd like to ask if repeated heating on embedded pellet affects staining as I wanted to make sure the pellet was at the bottom of agarose.? Are there other factors affect the staining. Any comments are welcomed. Thanks you in advance. Victor _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vhlwong <@t> yahoo.com Mon Feb 18 21:25:57 2013 From: vhlwong <@t> yahoo.com (Victor Wong) Date: Mon Feb 18 21:26:04 2013 Subject: [Histonet] Safranin O staining In-Reply-To: <6D6BD1DE8A5571489398B392A38A71579D23253C@xmdb04.nch.kids> References: <1361154852.62991.YahooMailNeo@web125202.mail.ne1.yahoo.com> <6D6BD1DE8A5571489398B392A38A71579D23231C@xmdb04.nch.kids> <1361172949.64418.YahooMailNeo@web125206.mail.ne1.yahoo.com> <6D6BD1DE8A5571489398B392A38A71579D23253C@xmdb04.nch.kids> Message-ID: <1361244357.35135.YahooMailNeo@web125202.mail.ne1.yahoo.com> Hi Tony, ? I'll extend the fixation, also as suggested by Rene. And then proceed to manual process.? Is automatic tissue processing fine with these samples??? It is interesting to process with albumin.? ? Both of the paper cannot be accessed in our lab and I'll try to find from other sources. ? P.S. unforuntuately we don't have a Haematology department?but may be we can try other cells. ? Best Regards, Victor ? From: Tony Henwood (SCHN) To: 'Victor Wong' ; "histonet@lists.utsouthwestern.edu" Sent: Tuesday, February 19, 2013 6:44 AM Subject: RE: [Histonet] Safranin O staining Hi Victor, ? I suppose the take home message from Russ?s paper (God rest his soul ? we do miss him) is that heat will affect some stains so extrapolating to routine processing temperatures, one could suppose that the deleterious effect would be more pronounced. To protect the cells, I would extend the fixation time and look at fixing before preparing the agar pellet. Or use the thrombin method instead (ask your Haematology department for expired thromboplastin from their clotting tests, use expired plasma from blood bank, add a few drops of 1% calcium chloride and a clot should form in 20seconds or so. Add NBF, fix for longer than an hour (4hr to overnight is better. And process as usual. Since the clots tend to be only about 3mm in greatest dimension (depending on the volume of thromboplastin and plasma used, a short gentle program will suffice (5 changes of alcohol, 20min each: 2 changes of xylene 30min each, 3 changes of wax, 30 min each). ? Alternatively you could mix the pellet with some egg albumin, add an equal volume of 10% formalin in alcohol, a nice clot will form, fix for a few hours and process from alcohol (Based on the paper, with modifications, by Yamamoto et al (1985) Am J Clin Pathol 83:409-414). ? Interstitial glycosaminoglycans and other mucins are also water soluble so fixing in formal alcohol should retain more of these ?mucins? than aqueous NBF fixation (Nathan, & van Deth (1983) Pathology 15:301-4) ? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 thechildren'shospitalat westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA ? From:Victor Wong [mailto:vhlwong@yahoo.com] Sent: Monday, 18 February 2013 6:36 PM To: Tony Henwood (SCHN); histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Safranin O staining ? Dear Tony, ? Thank you for your prompt reply and the paper. ? I do fix the cell before processing in agarose and after embedding in agarose. ? It is inevitable to process in wax at higher than 45C as it is set by the routine workers. ? It is the first time I worked on cell block precessing.? Do you have protocol?of processing chondrogenic pellet by?tissue processor?? Any adjustment to my procesing and staining protocol?? ? I tried to stain the pH1.0 alcian blue for 45 minutes and overnght but unremarkable staining (faint staining) was observed.? Do you think glycosaminoglycans secreted by chondrogenic pellet can be destroyed by heat? ? Best Regards, Victor ? From:Tony Henwood (SCHN) To: 'Victor Wong' ; "histonet@lists.utsouthwestern.edu" Sent: Monday, February 18, 2013 11:45 AM Subject: RE: [Histonet] Safranin O staining Victor, Heat can affect the way tissues and cells bind to dyes. I have attached an article that hints at some of these problems (Allison, R. T. and Bryant, D. (1998)'Effects of Processing at 45 C on Staining', Biotechnic and Histochemistry 73:3,128 -136). I have also found that some antigens are adversely affected by heat. CEA immunohistochemistry was decreased in cytology cell blocks made with molten agar compared to cell blocks made with fibrin clot. In both cases cell blocks were fixed in NBF after being made. Formalin seemed to protect the antigens in fibrin clots from subsequent heat damage during processing, whereas with agar cell blocks, the heat damage was already done. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victor Wong Sent: Monday, 18 February 2013 1:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Safranin O staining Hi all, I am working on induced chondrogenesis in cell culture.? After, induction, I put the clump of cells (pellets) in 2% agarose to make a cell block.? When the pellet did not sink to the bottom of agarose, I heated to melt the agarose again and centrifuged with higher speed.?I fix the agar block in 10% formalin for an hour and then transfer to 70% alcohol and proceeded with normal paraffin procedure.? I stained the paraffin sections with Sigma Safranin O stain but got very faint or no staining.? Here is my protocol: 1. Weigert Iron Hx for 2 min 2. Acid alcohol,Scott Tap and wash 3. 0.02% fast green for 2 min 4. 0.1% acetic acid for 1 min 5. Sigma's Safranin O for 5-10 min 6. 95% alcohol then 3x 100% alcohol @3 min 7. xylene and mount I also tried to stain the sections with alcian blue pH1.0 and the staining was not quite remarkable.? I'd like to ask if repeated heating on embedded pellet affects staining as I wanted to make sure the pellet was at the bottom of agarose.? Are there other factors affect the staining. Any comments are welcomed. Thanks you in advance. Victor _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From vhlwong <@t> yahoo.com Mon Feb 18 21:37:35 2013 From: vhlwong <@t> yahoo.com (Victor Wong) Date: Mon Feb 18 21:39:46 2013 Subject: [Histonet] Safranin O staining In-Reply-To: <14E2C6176416974295479C64A11CB9AE016411AB470A@SBS2K8.premierlab.local> References: <1361154852.62991.YahooMailNeo@web125202.mail.ne1.yahoo.com> <6D6BD1DE8A5571489398B392A38A71579D23231C@xmdb04.nch.kids>, <1361172949.64418.YahooMailNeo@web125206.mail.ne1.yahoo.com> <14E2C6176416974295479C64A11CB9AE016411AB470A@SBS2K8.premierlab.local> Message-ID: <1361245055.23166.YahooMailNeo@web125201.mail.ne1.yahoo.com> Hi?Liz, ? Thank you for your response and your invaluable experience in processing such samples.? I'll try manual processing on next batch of samples. ? I am new to?handle these samples.??There would be a lot of cell debris during culture mixing with small pellet and this may be much bigger than the pellet.? May I know how to get rid of it?? ? Someone suggested to stain the pellet with eosin and place it in folded?Kimwipe for processing.? Is routine automatic staining helpful as we don't have a oven to melt paraffin nearby?? We need to take a 30-minute walk to the lab for processing. ? BTW, I don't have access right to the paper. Anyway, the staining pictures on the website?are very nice.? ? Once, thank you for your help. ? Best Regards, Victor From: Elizabeth Chlipala To: Victor Wong ; Tony Henwood (SCHN) ; "histonet@lists.utsouthwestern.edu" Sent: Monday, February 18, 2013 11:37 PM Subject: RE: [Histonet] Safranin O staining We have processed and stained these types of samples before.? We do not embed the pellets in agarose prior to processing.? We dye them with eosin and place them in a tea bag and process on a very short cycle - 10 minutes per station, embed section and stain.? We have stained with safranin O, alcian blue, toluidine blue and IHC for collagen II.? The safranin O comes out just fine as well as the others. We just receive the samples so I do not know anything about the preparation, collection and fixation.? It possibly could be your construct, we have processed hundreds of these samples and the staining intensity and patterns do change.? I expect this is due to the type of preparation.? If you want to see pictures of the staining, you can go to this website: http://bioreagents.lifemapsc.com/products/purestem-chondropkg-4d20-8 or this paper: Sternberg H, Murai JT, Erickson IE, Funk WD, Das S, Wang Q, Snyder E, Chapman KB, Vangsness CT Jr, West MD. 2012 A human embryonic stem cell-derived clonal progenitor cell line with chondrogenic potential and markers of craniofacial mesenchyme. Regen Med. 7(4):481-501 Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 881-0763 cell (303) 682-9060 fax liz@premierlab.com Ship to address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victor Wong [vhlwong@yahoo.com] Sent: Monday, February 18, 2013 12:35 AM To: Tony Henwood (SCHN); histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Safranin O staining Dear Tony, Thank you for your prompt reply and the paper. I do fix the cell before processing in agarose and after embedding in agarose. It is inevitable to process in wax at higher than 45C as it is set by the routine workers. It is the first time I worked on cell block precessing.? Do you have protocol of processing chondrogenic pellet by tissue processor?? Any adjustment to my procesing and staining protocol? I tried to stain the pH1.0 alcian blue for 45 minutes and overnght but unremarkable staining (faint staining) was observed.? Do you think glycosaminoglycans secreted by chondrogenic pellet can be destroyed by heat? Best Regards, Victor From: Tony Henwood (SCHN) To: 'Victor Wong' ; "histonet@lists.utsouthwestern.edu" Sent: Monday, February 18, 2013 11:45 AM Subject: RE: [Histonet] Safranin O staining Victor, Heat can affect the way tissues and cells bind to dyes. I have attached an article that hints at some of these problems (Allison, R. T. and Bryant, D. (1998)'Effects of Processing at 45 C on Staining', Biotechnic and Histochemistry 73:3,128 -136). I have also found that some antigens are adversely affected by heat. CEA immunohistochemistry was decreased in cytology cell blocks made with molten agar compared to cell blocks made with fibrin clot. In both cases cell blocks were fixed in NBF after being made. Formalin seemed to protect the antigens in fibrin clots from subsequent heat damage during processing, whereas with agar cell blocks, the heat damage was already done. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victor Wong Sent: Monday, 18 February 2013 1:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Safranin O staining Hi all, I am working on induced chondrogenesis in cell culture.? After, induction, I put the clump of cells (pellets) in 2% agarose to make a cell block.? When the pellet did not sink to the bottom of agarose, I heated to melt the agarose again and centrifuged with higher speed. I fix the agar block in 10% formalin for an hour and then transfer to 70% alcohol and proceeded with normal paraffin procedure.? I stained the paraffin sections with Sigma Safranin O stain but got very faint or no staining.? Here is my protocol: 1. Weigert Iron Hx for 2 min 2. Acid alcohol,Scott Tap and wash 3. 0.02% fast green for 2 min 4. 0.1% acetic acid for 1 min 5. Sigma's Safranin O for 5-10 min 6. 95% alcohol then 3x 100% alcohol @3 min 7. xylene and mount I also tried to stain the sections with alcian blue pH1.0 and the staining was not quite remarkable.? I'd like to ask if repeated heating on embedded pellet affects staining as I wanted to make sure the pellet was at the bottom of agarose.? Are there other factors affect the staining. Any comments are welcomed. Thanks you in advance. Victor _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hans <@t> histologistics.com Tue Feb 19 09:57:26 2013 From: hans <@t> histologistics.com (Hans B Snyder) Date: Tue Feb 19 09:57:43 2013 Subject: [Histonet] granuloma and lymphocytes with special stains Message-ID: Hello, Does anyone have a good special stain protocol for lymphocytes and or granulocytes? I have been trying PAS, Giemsa and T-Blue but the results are not specific enough. The tissue is rat hearts from 3 years ago so the antigenicy is all but gone for IHC. Thank you Hans B Snyder Histologistics 60 Prescott Street Worcester, MA 01605 508-308-7800 hans@histologistics.com From rjbuesa <@t> yahoo.com Tue Feb 19 10:07:52 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Feb 19 10:07:58 2013 Subject: [Histonet] granuloma and lymphocytes with special stains In-Reply-To: References: Message-ID: <1361290072.75677.YahooMailNeo@web163102.mail.bf1.yahoo.com> Giemsa is the adequate protocol but perhaps your protocol is not good enough. Under separate cover I am sending mine. Ren? J. From: Hans B Snyder To: Histonet@lists.utsouthwestern.edu Sent: Tuesday, February 19, 2013 10:57 AM Subject: [Histonet] granuloma and lymphocytes with special stains Hello, Does anyone have a good special stain protocol for lymphocytes and or granulocytes? I have been trying PAS, Giemsa and T-Blue but the results are not specific enough. The tissue is rat hearts from 3 years ago so the antigenicy is all but gone for IHC. Thank you Hans B Snyder Histologistics 60 Prescott Street Worcester, MA 01605 508-308-7800 hans@histologistics.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Tue Feb 19 10:15:42 2013 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Tue Feb 19 10:15:54 2013 Subject: [Histonet] Safranin O staining In-Reply-To: <1361245055.23166.YahooMailNeo@web125201.mail.ne1.yahoo.com> References: <1361154852.62991.YahooMailNeo@web125202.mail.ne1.yahoo.com> <6D6BD1DE8A5571489398B392A38A71579D23231C@xmdb04.nch.kids>, <1361172949.64418.YahooMailNeo@web125206.mail.ne1.yahoo.com> <14E2C6176416974295479C64A11CB9AE016411AB470A@SBS2K8.premierlab.local>, <1361245055.23166.YahooMailNeo@web125201.mail.ne1.yahoo.com> Message-ID: <14E2C6176416974295479C64A11CB9AE016411AB471D@SBS2K8.premierlab.local> Victor We process these samples on the tissue processor and not manually. You can use a kimwipe but we prefer the nylon tissue bags (the small ones 30 x 50 mm), they are a bit more expensive but they work well for us. We receive the pellets in a conical tube, we add a bit of eosin, about a ml, to the formalin in the tube and let it sit for about 10 minutes. We then use disposable pipets to retrieve the pellet from the conical tube, we don't use forceps at this stage, we draw up the pellet into the conical tube, open the tissue bag and then squirt the solution and pellet into the bag. Its helpful to have the pellet stained with eosin this way you can actually see it. We fold the bag and place it into the cassette. We embed in the pellets in the 15x15 mm base molds. We capture 4 levels per slide for a total of 8 sections, since the samples are so tiny we collect all the tissue onto unstained slides, this allows us to have sections through the pellet. What we do is collect several ribbions of tissue with about 15-20 sections per ribbon and then pick up 2 sections at a time. I will attach a diagram from our technical manual to help out in another e-mail. I'll see if I can get access to the paper. I can't comment on how to get rid of the debris since we do not perform that process. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 881-0763 cell (303) 682-9060 fax liz@premierlab.com Ship to address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 ________________________________ From: Victor Wong [vhlwong@yahoo.com] Sent: Monday, February 18, 2013 8:37 PM To: Elizabeth Chlipala; Tony Henwood (SCHN); histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Safranin O staining Hi Liz, Thank you for your response and your invaluable experience in processing such samples. I'll try manual processing on next batch of samples. I am new to handle these samples. There would be a lot of cell debris during culture mixing with small pellet and this may be much bigger than the pellet. May I know how to get rid of it? Someone suggested to stain the pellet with eosin and place it in folded Kimwipe for processing. Is routine automatic staining helpful as we don't have a oven to melt paraffin nearby? We need to take a 30-minute walk to the lab for processing. BTW, I don't have access right to the paper. Anyway, the staining pictures on the website are very nice. Once, thank you for your help. Best Regards, Victor From: Elizabeth Chlipala To: Victor Wong ; Tony Henwood (SCHN) ; "histonet@lists.utsouthwestern.edu" Sent: Monday, February 18, 2013 11:37 PM Subject: RE: [Histonet] Safranin O staining We have processed and stained these types of samples before. We do not embed the pellets in agarose prior to processing. We dye them with eosin and place them in a tea bag and process on a very short cycle - 10 minutes per station, embed section and stain. We have stained with safranin O, alcian blue, toluidine blue and IHC for collagen II. The safranin O comes out just fine as well as the others. We just receive the samples so I do not know anything about the preparation, collection and fixation. It possibly could be your construct, we have processed hundreds of these samples and the staining intensity and patterns do change. I expect this is due to the type of preparation. If you want to see pictures of the staining, you can go to this website: http://bioreagents.lifemapsc.com/products/purestem-chondropkg-4d20-8 or this paper: Sternberg H, Murai JT, Erickson IE, Funk WD, Das S, Wang Q, Snyder E, Chapman KB, Vangsness CT Jr, West MD. 2012 A human embryonic stem cell-derived clonal progenitor cell line with chondrogenic potential and markers of craniofacial mesenchyme. Regen Med. 7(4):481-501 Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 881-0763 cell (303) 682-9060 fax liz@premierlab.com Ship to address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victor Wong [vhlwong@yahoo.com] Sent: Monday, February 18, 2013 12:35 AM To: Tony Henwood (SCHN); histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Safranin O staining Dear Tony, Thank you for your prompt reply and the paper. I do fix the cell before processing in agarose and after embedding in agarose. It is inevitable to process in wax at higher than 45C as it is set by the routine workers. It is the first time I worked on cell block precessing. Do you have protocol of processing chondrogenic pellet by tissue processor? Any adjustment to my procesing and staining protocol? I tried to stain the pH1.0 alcian blue for 45 minutes and overnght but unremarkable staining (faint staining) was observed. Do you think glycosaminoglycans secreted by chondrogenic pellet can be destroyed by heat? Best Regards, Victor From: Tony Henwood (SCHN) > To: 'Victor Wong' >; "histonet@lists.utsouthwestern.edu" > Sent: Monday, February 18, 2013 11:45 AM Subject: RE: [Histonet] Safranin O staining Victor, Heat can affect the way tissues and cells bind to dyes. I have attached an article that hints at some of these problems (Allison, R. T. and Bryant, D. (1998)'Effects of Processing at 45 C on Staining', Biotechnic and Histochemistry 73:3,128 -136). I have also found that some antigens are adversely affected by heat. CEA immunohistochemistry was decreased in cytology cell blocks made with molten agar compared to cell blocks made with fibrin clot. In both cases cell blocks were fixed in NBF after being made. Formalin seemed to protect the antigens in fibrin clots from subsequent heat damage during processing, whereas with agar cell blocks, the heat damage was already done. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victor Wong Sent: Monday, 18 February 2013 1:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Safranin O staining Hi all, I am working on induced chondrogenesis in cell culture. After, induction, I put the clump of cells (pellets) in 2% agarose to make a cell block. When the pellet did not sink to the bottom of agarose, I heated to melt the agarose again and centrifuged with higher speed. I fix the agar block in 10% formalin for an hour and then transfer to 70% alcohol and proceeded with normal paraffin procedure. I stained the paraffin sections with Sigma Safranin O stain but got very faint or no staining. Here is my protocol: 1. Weigert Iron Hx for 2 min 2. Acid alcohol,Scott Tap and wash 3. 0.02% fast green for 2 min 4. 0.1% acetic acid for 1 min 5. Sigma's Safranin O for 5-10 min 6. 95% alcohol then 3x 100% alcohol @3 min 7. xylene and mount I also tried to stain the sections with alcian blue pH1.0 and the staining was not quite remarkable. I'd like to ask if repeated heating on embedded pellet affects staining as I wanted to make sure the pellet was at the bottom of agarose. Are there other factors affect the staining. Any comments are welcomed. Thanks you in advance. Victor _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Tue Feb 19 15:18:03 2013 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Tue Feb 19 15:18:57 2013 Subject: [Histonet] Help! Message-ID: <5123A5BB.7770.0077.1@harthosp.org> I need to speak with someone who has experience with doing (and interpretating) in situ hybridization for EBER. Please contact me directly. Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-2204 Fax From vhlwong <@t> yahoo.com Wed Feb 20 01:52:25 2013 From: vhlwong <@t> yahoo.com (Victor Wong) Date: Wed Feb 20 01:52:36 2013 Subject: [Histonet] Safranin O staining In-Reply-To: <14E2C6176416974295479C64A11CB9AE016411AB471D@SBS2K8.premierlab.local> References: <1361154852.62991.YahooMailNeo@web125202.mail.ne1.yahoo.com> <6D6BD1DE8A5571489398B392A38A71579D23231C@xmdb04.nch.kids>, <1361172949.64418.YahooMailNeo@web125206.mail.ne1.yahoo.com> <14E2C6176416974295479C64A11CB9AE016411AB470A@SBS2K8.premierlab.local>, <1361245055.23166.YahooMailNeo@web125201.mail.ne1.yahoo.com> <14E2C6176416974295479C64A11CB9AE016411AB471D@SBS2K8.premierlab.local> Message-ID: <1361346745.77420.YahooMailNeo@web125204.mail.ne1.yahoo.com> Hi Liz, ? Get it.? Thank you for the detailed procedures. ? Best Regards, Victor From: Elizabeth Chlipala To: Victor Wong ; Tony Henwood (SCHN) ; "histonet@lists.utsouthwestern.edu" Sent: Wednesday, February 20, 2013 12:15 AM Subject: RE: [Histonet] Safranin O staining Victor ? We process these samples on the tissue processor and not manually.? You can use a kimwipe but we prefer the nylon tissue bags (the small ones 30 x 50 mm), they are a bit more expensive but they work well for us.? We receive the pellets in a conical tube, we add a bit of eosin, about a ml, to the formalin in the tube and let it sit for about 10 minutes.? We then use disposable pipets to retrieve the pellet from the conical tube, we don't use forceps at this stage, we?draw up the pellet into the conical tube, open the tissue bag and then squirt the solution and pellet into the bag.? Its helpful to have the pellet stained with eosin this way you can actually see it.? We fold the bag and place it into the cassette.? We embed in the pellets in the 15x15 mm base molds.? We capture 4 levels per slide for a total of 8 sections, since the samples are so tiny we collect all the tissue onto unstained slides, this allows us to have sections through the pellet.? What we do is collect several ribbions of tissue with about 15-20 sections per ribbon and then pick up 2 sections at a time.? I will attach a diagram from our technical manual to help out in another e-mail.? I'll see if I can get access to the paper.? I can't comment on how to get rid of the debris since we do not perform that process. ? Liz ? Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 881-0763 cell (303) 682-9060 fax liz@premierlab.com ? Ship to address: ? Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 From: Victor Wong [vhlwong@yahoo.com] Sent: Monday, February 18, 2013 8:37 PM To: Elizabeth Chlipala; Tony Henwood (SCHN); histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Safranin O staining Hi?Liz, ? Thank you for your response and your invaluable experience in processing such samples.? I'll try manual processing on next batch of samples. ? I am new to?handle these samples.??There would be a lot of cell debris during culture mixing with small pellet and this may be much bigger than the pellet.? May I know how to get rid of it?? ? Someone suggested to stain the pellet with eosin and place it in folded?Kimwipe for processing.? Is routine automatic staining helpful as we don't have a oven to melt paraffin nearby?? We need to take a 30-minute walk to the lab for processing. ? BTW, I don't have access right to the paper. Anyway, the staining pictures on the website?are very nice.? ? Once, thank you for your help. ? Best Regards, Victor From: Elizabeth Chlipala To: Victor Wong ; Tony Henwood (SCHN) ; "histonet@lists.utsouthwestern.edu" Sent: Monday, February 18, 2013 11:37 PM Subject: RE: [Histonet] Safranin O staining We have processed and stained these types of samples before.? We do not embed the pellets in agarose prior to processing.? We dye them with eosin and place them in a tea bag and process on a very short cycle - 10 minutes per station, embed section and stain.? We have stained with safranin O, alcian blue, toluidine blue and IHC for collagen II.? The safranin O comes out just fine as well as the others. We just receive the samples so I do not know anything about the preparation, collection and fixation.? It possibly could be your construct, we have processed hundreds of these samples and the staining intensity and patterns do change.? I expect this is due to the type of preparation.? If you want to see pictures of the staining, you can go to this website: http://bioreagents.lifemapsc.com/products/purestem-chondropkg-4d20-8 or this paper: Sternberg H, Murai JT, Erickson IE, Funk WD, Das S, Wang Q, Snyder E, Chapman KB, Vangsness CT Jr, West MD. 2012 A human embryonic stem cell-derived clonal progenitor cell line with chondrogenic potential and markers of craniofacial mesenchyme. Regen Med. 7(4):481-501 Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 881-0763 cell (303) 682-9060 fax liz@premierlab.com Ship to address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victor Wong [vhlwong@yahoo.com] Sent: Monday, February 18, 2013 12:35 AM To: Tony Henwood (SCHN); histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Safranin O staining Dear Tony, Thank you for your prompt reply and the paper. I do fix the cell before processing in agarose and after embedding in agarose. It is inevitable to process in wax at higher than 45C as it is set by the routine workers. It is the first time I worked on cell block precessing.? Do you have protocol of processing chondrogenic pellet by tissue processor?? Any adjustment to my procesing and staining protocol? I tried to stain the pH1.0 alcian blue for 45 minutes and overnght but unremarkable staining (faint staining) was observed.? Do you think glycosaminoglycans secreted by chondrogenic pellet can be destroyed by heat? Best Regards, Victor From: Tony Henwood (SCHN) To: 'Victor Wong' ; "histonet@lists.utsouthwestern.edu" Sent: Monday, February 18, 2013 11:45 AM Subject: RE: [Histonet] Safranin O staining Victor, Heat can affect the way tissues and cells bind to dyes. I have attached an article that hints at some of these problems (Allison, R. T. and Bryant, D. (1998)'Effects of Processing at 45 C on Staining', Biotechnic and Histochemistry 73:3,128 -136). I have also found that some antigens are adversely affected by heat. CEA immunohistochemistry was decreased in cytology cell blocks made with molten agar compared to cell blocks made with fibrin clot. In both cases cell blocks were fixed in NBF after being made. Formalin seemed to protect the antigens in fibrin clots from subsequent heat damage during processing, whereas with agar cell blocks, the heat damage was already done. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victor Wong Sent: Monday, 18 February 2013 1:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Safranin O staining Hi all, I am working on induced chondrogenesis in cell culture.? After, induction, I put the clump of cells (pellets) in 2% agarose to make a cell block.? When the pellet did not sink to the bottom of agarose, I heated to melt the agarose again and centrifuged with higher speed. I fix the agar block in 10% formalin for an hour and then transfer to 70% alcohol and proceeded with normal paraffin procedure.? I stained the paraffin sections with Sigma Safranin O stain but got very faint or no staining.? Here is my protocol: 1. Weigert Iron Hx for 2 min 2. Acid alcohol,Scott Tap and wash 3. 0.02% fast green for 2 min 4. 0.1% acetic acid for 1 min 5. Sigma's Safranin O for 5-10 min 6. 95% alcohol then 3x 100% alcohol @3 min 7. xylene and mount I also tried to stain the sections with alcian blue pH1.0 and the staining was not quite remarkable.? I'd like to ask if repeated heating on embedded pellet affects staining as I wanted to make sure the pellet was at the bottom of agarose.? Are there other factors affect the staining. Any comments are welcomed. Thanks you in advance. Victor _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BZIMMERM <@t> gru.edu Wed Feb 20 08:22:53 2013 From: BZIMMERM <@t> gru.edu (Zimmerman, Billie) Date: Wed Feb 20 08:23:31 2013 Subject: [Histonet] Early Registration for Georgia Society for Histotechnology Symposium Message-ID: <7B3DEB32E69C034EACB479059C5DE3FF75688B@EX-MLB-03.ad.georgiahealth.edu> Early registration for NSH CEU's is only $115 if registered by March 1, 2013. Please join us for a scenic weekend on the beach. Jekyll is one of four Golden Isles of Georgia and if you enjoy the outdoors, there are bike trails and lots of wildlife. It should prove to be an educational mini-vacation. Meet us at the Oceanside Inn and Suites, April 12 - 14, 2013. Wanda Simons/bz GSH President Augusta State University and Georgia Health Sciences University have consolidated to become Georgia Regents University. Effective January 9, 2013, my email address has changed to BZIMMERM@gru.edu. Please update your address book to reflect this change. From Elizabeth.Cameron <@t> jax.org Wed Feb 20 09:05:25 2013 From: Elizabeth.Cameron <@t> jax.org (Elizabeth Cameron) Date: Wed Feb 20 09:05:35 2013 Subject: [Histonet] Benchmarking - Please Help! Message-ID: Hello Histonetters, I am once again requesting that you take some time out of your day to help our facility (and yours!) with a benchmarking survey. It only takes a few minutes, and the results will be (anonymously) shared with all survey participants. If you work in research, a hospital, industry, or any other setting we want your participation! We are trying to gather as much information as possible before February 28th, and to date we only have 6 participants. I know there are more than 6 histonetters.... Thanks in advance for your help, and thank you to the 6 participants who have completed the survey! Here is the link: http://www.surveymonkey.com/s/HistologySurvey2013 Elizabeth M. Cameron, HT, QIHC (ASCP) Histology Supervisor The Jackson Laboratory Bar Harbor, Maine 207-288-6326 The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as possible. From jshelley <@t> sanfordburnham.org Wed Feb 20 09:45:58 2013 From: jshelley <@t> sanfordburnham.org (John Shelley) Date: Wed Feb 20 09:46:05 2013 Subject: [Histonet] Ventana FISH Protocol Message-ID: <5A605CE38EECB64B94485C02125A0C44351C97ED62@LN-MAIL07.ln.burnham.org> Hi Netters, Hope everyone is having a good day!!! I have a group that is interested in running FISH on prostate tissue samples and we have only run this on cells. I wanted to use someone's protocol from the Ventana Benchmark/Ultra systems that they are using for their breast tissue when doing FISH. I know that there would possible be more optimizing but wanted it as a starting point. Any help would be greatly appreciated. If you do not mind sharing and sending off-line your protocols that would be great. Thanks in advance!!! Kind Regards! John J Shelley From Pamela.Johnson <@t> STJUDE.ORG Wed Feb 20 10:23:14 2013 From: Pamela.Johnson <@t> STJUDE.ORG (Johnson, Pamela) Date: Wed Feb 20 10:24:07 2013 Subject: [Histonet] Re: [IHCRG] CD8 on mouse tissue In-Reply-To: <4895A1696F956D4CB56011A8C61312820E56D5452C@ushpwbmsmmp008.one.ads.bms.com> References: <008a01ce0a27$e815ace0$b84106a0$@ihctech.net> <1A7CC3E7-B91C-484D-BC0D-CF1C72DF1E3E@u.washington.edu> <00a501ce0a2c$b9be0540$2d3a0fc0$@ihctech.net> <8EDCE182006F9448A3D04D7ED38B238101AF357E@BY2PRD0712MB607.namprd07.prod.outlook.com> <00cc01ce0a6a$38097c80$a81c7580$@ihctech.net> <8EDCE182006F9448A3D04D7ED38B238101AF3745@BY2PRD0712MB607.namprd07.prod.outlook.com> <004201ce0ad0$7ca693d0$75f3bb70$@ihctech.net> <8EDCE182006F9448A3D04D7ED38B238101AF38BB@BY2PRD0712MB607.namprd07.prod.outlook.com> <009c01ce0af5$ca651310$5f2f3930$@ihctech.net> <00ad01ce0af6$53ba6480$fb2f2d80$@ihctech.net> <4895A1696F956D4CB56011A8C61312820E56D5452C@ushpwbmsmmp008.one.ads.bms.com> Message-ID: <9C451702-C302-4620-B267-C606E8112BD7@STJUDE.ORG> Anne, How long do you leave your tissue in the fixative? If you have a protocol you are willing to share it would be most appreciated. Thanks!! Pam Sent from my iPhone On Feb 20, 2013, at 9:32 AM, "Lewin, Anne" > wrote: We have used the zinc fixation method with some success, but you have to be VERY careful of the amount of time the tissue is in Zinc fix (NOT Zinc Formalin). Also, make sure that your tissue processor has no trace of formalin in the lines or in the first alcohols during processing. -Anne From: ihcrg@googlegroups.com [mailto:ihcrg@googlegroups.com] On Behalf Of pruegg@ihctech.net Sent: Thursday, February 14, 2013 4:01 PM To: ihcrg@googlegroups.com; histonet@lists.utsouthwestern.edu Subject: FW: [IHCRG] CD8 on mouse tissue Here is the reference for zinc salt fixed mouse tissue labeled for cd4/8 PE, it looks really promising. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E. Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 rueggihcconsultingpr@outlook.com This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. From: pruegg@ihctech.net [mailto:pruegg@ihctech.net] Sent: Thursday, February 14, 2013 1:57 PM To: pruegg@ihctech.net Subject: FW: [IHCRG] CD8 on mouse tissue Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E. Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 rueggihcconsultingpr@outlook.com This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. From: AJ Milici [mailto:amilici@flagshipbio.com] Sent: Thursday, February 14, 2013 11:56 AM To: pruegg@ihctech.net; Sherri Saturley Subject: RE: [IHCRG] CD8 on mouse tissue This will give you some info on my warped logic. AJ A.J. Milici, Ph.D. Director of Neurobiology and Inflammation Flagship Biosciences email: amilici@flagshipbio.com Office: (203) 627-3369 www.flagshipbio.com This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message. You may contact us at Flagship Biosciences LLC, 10955 Westmoor Dr, Suite 400, Westminster, CO 80021. Phone: 303-325-5894 From: pruegg@ihctech.net [mailto:pruegg@ihctech.net] Sent: Thursday, February 14, 2013 11:30 AM To: AJ Milici; Sherri Saturley Subject: RE: [IHCRG] CD8 on mouse tissue The snap frozen in OCT tissue is fixed after sectioning in acetone/alcohol instead of formaldehyde, that is what I mean by non-aldehyde fixation. CD4 and CD8 on murine tissue does not like formalin fixation and paraffin processing, which is why the golden standard for those two is snap frozen murine tissue acetone/ethanol fixed. I am looking into using zinc and/or PLP fixation for this. Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E. Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 rueggihcconsultingpr@outlook.com This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. From: AJ Milici [mailto:amilici@flagshipbio.com] Sent: Thursday, February 14, 2013 7:47 AM To: pruegg@ihctech.net; Sherri Saturley Subject: RE: [IHCRG] CD8 on mouse tissue Patsy: I will start putting together my proposal and you should get it by Monday. I have one question for you based upon what you wrote below ? exactly what do you mean by non-aldehyde fixed frozen samples. Do you mean fresh frozen sections that are fixed on the slide with cold MeOH or cold acetone? Thanks, AJ A.J. Milici, Ph.D. Director of Neurobiology and Inflammation Flagship Biosciences email: amilici@flagshipbio.com Office: (203) 627-3369 www.flagshipbio.com This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message. You may contact us at Flagship Biosciences LLC, 10955 Westmoor Dr, Suite 400, Westminster, CO 80021. Phone: 303-325-5894 From: pruegg@ihctech.net [mailto:pruegg@ihctech.net] Sent: Wednesday, February 13, 2013 11:18 PM To: AJ Milici; Sherri Saturley Subject: RE: [IHCRG] CD8 on mouse tissue We have done a great deal of CD8 localization in murine tissue but it has all been done on non aldehyde fixed frozen tissue, we have actually tried several antibodies on ffpe murine tissue for cd8 (the work we did for Roche/ John Woods) but did not have success. I will inquire about zinc fixed samples and plp fixed samples for cd8 expression in murine tissues, I have heard of this but have not done it myself. If you have some references for zinc fixed murine tissues IHC stained for cd8 please forward those to us. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E. Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 rueggihcconsultingpr@outlook.com This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. From: AJ Milici [mailto:amilici@flagshipbio.com] Sent: Wednesday, February 13, 2013 2:47 PM To: pruegg@ihctech.net; Sherri Saturley Subject: RE: [IHCRG] CD8 on mouse tissue I fully realize that CD8 localization in FFPE will not work. However, there is some interesting data out there using a zinc fixative or PLP. While Frozen sections will work for the IHC they may be limiting in terms of the image analysis due to thicker sections and decreased morphological preservation. I will lay out my data for you to poke holes in it. So did you ever do much in the way of CD8 localization on murine tissue? AJ A.J. Milici, Ph.D. Director of Neurobiology and Inflammation Flagship Biosciences email: amilici@flagshipbio.com Office: (203) 627-3369 www.flagshipbio.com This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message. You may contact us at Flagship Biosciences LLC, 10955 Westmoor Dr, Suite 400, Westminster, CO 80021. Phone: 303-325-5894 From: pruegg@ihctech.net [mailto:pruegg@ihctech.net] Sent: Wednesday, February 13, 2013 3:57 PM To: Sherri Saturley; AJ Milici Subject: FW: [IHCRG] CD8 on mouse tissue I am inquiring about advances in CD8 IHC on mouse tissue, here is one report, just as I thought, still. AJ do you want to do this on formalin fixed paraffin embedded mouse tissue? The standard for CD4 and CD8 still seems to be rat anti mouse ab on frozen samples not aldehyde fixed. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E. Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 rueggihcconsultingpr@outlook.com This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. From: Brian Johnson [mailto:brianj18@u.washington.edu] Sent: Wednesday, February 13, 2013 1:30 PM To: pruegg@ihctech.net Subject: Re: [IHCRG] CD8 on mouse tissue I just recently worked this up on Fresh Frozen vs. fixed, sucrose post fixed frozen and FFPE tissue. Only worked on fresh frozen. I use the BD marker that everyone swears by. Same results for CD4. I can give you more details if you are interested. Brian Brian Johnson Program Manager U.W. at South Lake Union, N310 HIC/Comparative Pathology Program 850 Republican St Seattle, WA 98109-4725 Work Phone: 206-685-6517 Work E-mail: brianj18@uw.edu www.uwhistologyandimaging.org On Feb 13, 2013, at 12:22 PM, > wrote: Is this still being done on frozen sections not aldehyde fixed or has someone figured out an antibody or method of doing it on FFPE mouse tissue yet??? Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E. Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 rueggihcconsultingpr@outlook.com This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. -- -- You received this message because you are subscribed to the Google Groups "ihcrg" group. The IHC Resource Group is a standing committee within the National Society for Histotechnology. To post to this group, send email to ihcrg@googlegroups.com To unsubscribe from this group, send email to ihcrg+unsubscribe@googlegroups.com For more options, visit this group at http://groups.google.com/group/ihcrg?hl=en To contact the National Society for Histotechnology, email: histo@nsh.org or call 443.535.4060. --- You received this message because you are subscribed to the Google Groups "ihcrg" group. 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For more options, visit https://groups.google.com/groups/opt_out. ________________________________ This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. -- -- You received this message because you are subscribed to the Google Groups "ihcrg" group. The IHC Resource Group is a standing committee within the National Society for Histotechnology. 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For more options, visit https://groups.google.com/groups/opt_out. ________________________________ Email Disclaimer: www.stjude.org/emaildisclaimer Consultation Disclaimer: www.stjude.org/consultationdisclaimer From PEROWES10 <@t> juniata.edu Wed Feb 20 11:20:10 2013 From: PEROWES10 <@t> juniata.edu (Perow, Elliott S (PEROWES10)) Date: Wed Feb 20 11:20:16 2013 Subject: [Histonet] Manual for Leica Cryocut 1800 Message-ID: Hello All, I was wondering if anyone has and is willing to share a manual for a Leica Cryocut 1800 with a Reichert-Jung 2020 microtome? I am trying to get one up and running to section brook trout brain. Any help would be greatly appreciated. Thanks, Elliott Perow Juniata College Biology POE CONFIDENTIALITY NOTICE: The materials in this electronic mail transmission (including all attachments) are private and confidential and are the property of the sender. The information contained in the material is privileged and is intended only for the use of the named addressee(s). If you are not the intended addressee, be advised that any unauthorized disclosure, copying, distribution or the taking of any action in reliance on the contents of this material is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender by replying to the e-mail, and then destroy it immediately. Thank you. From relia1 <@t> earthlink.net Wed Feb 20 11:22:23 2013 From: relia1 <@t> earthlink.net (Pam Barker) Date: Wed Feb 20 11:22:26 2013 Subject: [Histonet] RELIA Histology Careers Bulletin 2-20-2013 Please read especially if you love your job! Message-ID: <0da201ce0f8e$d8f8f4d0$8aeade70$@earthlink.net> Hi Group Members! Recently I wrote a blog piece entitled ?Would You Want You for Coworker?? The article talked about the things that make someone a good coworker. The article got me thinking Wouldn?t you like to have input on who is hired in your lab? I realize that a lot of the things in the article are applicable to most lab environments but I also realize that each lab is unique with its own culture and chemistry (no pun intended) among its employees. That being said my hypothesis is that the best person to ask about what would make a great employee in your lab is you. So I am expanding my referral program to include job leads. If you have an opening in your lab for a histotech or a med tech let me know. If you can provide the name and email address of the hiring authority and I place a candidate in the position I will pay you a referral fee of $500.00 Yes that means you can help find the right person for your lab and make a little spending cash! 1. Get me the job lead and contact information ? email address preferred. 2. Let?s chat- you and I about what you want in a coworker. 3. I place a tech in your lab with you who you are going to enjoy working with 4. You earn a referral fee. I also wanted to let you know I have some interesting positions in NC, PA, CA, VA, NH, ME and KY. All of these are permanent full time positions with companies that offer excellent compensation, great benefits and an outstanding work environment. If you are interested in hearing more about job opportunities in these or any other areas call or email me. Toll free 866-607-3542 email: relia1@earthlink.net If you know of an opening that I can help with contact me right away too!! Thanks and I hope you have a wonderful week!! Pam Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker ? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From denise.long <@t> uconn.edu Wed Feb 20 12:34:27 2013 From: denise.long <@t> uconn.edu (Long, Denise) Date: Wed Feb 20 12:35:40 2013 Subject: [Histonet] Prox-1 antibody Message-ID: <98AE5DDFDD7D204E9CE6C7AE28C0B27B99FEC4@MAILD.grove.ad.uconn.edu> Is anyone out there using Prox-1 antibody? In particular, clone 5G10. Please contact off the list-serv. Thanks, Denise Denise M. Long, MS, HTL (ASCP), QIHC University of Connecticut Dept. of Pathobiology and Veterinary Sciences Connecticut Veterinary Medical Diagnostic Laboratory 61 N. Eagleville Road, Unit 3089 Storrs, Connecticut 06269-3089 (860) 486-0851 No trees were harmed in the sending of this email, however a few electrons were temporarily redirected for its purpose. From NMargaryan <@t> luriechildrens.org Wed Feb 20 16:02:02 2013 From: NMargaryan <@t> luriechildrens.org (Margaryan, Naira) Date: Wed Feb 20 16:05:00 2013 Subject: [Histonet] zebrafish and IHC Message-ID: <34B2EDA118548A4EB35D6E650345BA641F678B3C@SV-EX08.childrensmemorial.org> Dear Histonetters, I am trying to do IHC on FFPE 4 weeks old zebrafish with different Abs. Is there a trick working with zebrafish? I am using the same IHC protocol I always use on human and mouse tissue and my Abs are suppose to work on fish as well as human. I run human and fish sections together with same AR and IHC protocol. By the end I get beautiful staining on human section and or nothing or some fuzzy/fuggy/unclarified/undistinguished reaction. Any help is appreciated as I am new in fish field, Naira From kjgada <@t> gmail.com Wed Feb 20 18:32:36 2013 From: kjgada <@t> gmail.com (Komal Gada) Date: Wed Feb 20 18:34:42 2013 Subject: [Histonet] Part Time Histolgy Faculty Needed - Orlando, FL Message-ID: Hello all, I'm writing to inform anyone interested in a part-time Histology faculty position in Orlando, FL available immediately. The person will instruct students in the part-time program on Monday, Tuesday and Thursday evenings. The position requires someone with at least 3 - 5 years of experience as a Histotechnologist or Histotechnician with appropriate credentials from ASCP (HT or HTL). If you are interested or have any questions, please email me. Also, if you know anyone that may be interested, please pass this along. Thanks all, Komal Gada, HT / HTL (ASCP), QIHC From lucy.bell <@t> uct.ac.za Thu Feb 21 04:46:23 2013 From: lucy.bell <@t> uct.ac.za (Lucy Bell) Date: Thu Feb 21 04:46:39 2013 Subject: [Histonet] Storage of formalin fixed tissue Message-ID: <5126171F020000BF001D0469@gwiasmtp.uct.ac.za> Hello, I am looking for some advice about medium-long term storage of formalin fixed samples. I am collecting small skin samples (4mmx4mm max) in South Africa and am planning to fix them immediately in 10%NBF. However I am then not able to process them until approximately three months subsequently. What is the best storage option in this regard? Keeping them in 10%NBF at 4 degrees Celsius? I will eventually want to perform H&E staining and potentially some immunohistochemistry. Thanks and regards ### UNIVERSITY OF CAPE TOWN This e-mail is subject to the UCT ICT policies and e-mail disclaimer published on our website at http://www.uct.ac.za/about/policies/emaildisclaimer/ or obtainable from +27 21 650 9111. This e-mail is intended only for the person(s) to whom it is addressed. If the e-mail has reached you in error, please notify the author. If you are not the intended recipient of the e-mail you may not use, disclose, copy, redirect or print the content. If this e-mail is not related to the business of UCT it is sent by the sender in the sender's individual capacity. ### ________________________________ UNIVERSITY OF CAPE TOWN This e-mail is subject to the UCT ICT policies and e-mail disclaimer published on our website at http://www.uct.ac.za/about/policies/emaildisclaimer/ or obtainable from +27 21 650 9111. This e-mail is intended only for the person(s) to whom it is addressed. If the e-mail has reached you in error, please notify the author. If you are not the intended recipient of the e-mail you may not use, disclose, copy, redirect or print the content. If this e-mail is not related to the business of UCT it is sent by the sender in the sender's individual capacity. From Sarah_Mack <@t> urmc.rochester.edu Thu Feb 21 07:44:08 2013 From: Sarah_Mack <@t> urmc.rochester.edu (Mack, Sarah) Date: Thu Feb 21 07:45:08 2013 Subject: [Histonet] NYSHS 2013 Annual Symposium Message-ID: The annual NYSHS meeting will be held at SUNY Cobleskill, April 13th 2013. The one day symposium entitled "Future of Histology and Anatomic Pathology" has an excellent educational program. We are honored to have Beth Sheppard, President of NSH, Past President of NYSHS as well as Fellow of NYSHS give the Key Note Presentation this year. Please visit for more information: http://www.nyhisto.org/meetings/current-nys-meeting/educational-program/ http://www.nyhisto.org/meetings/current-nys-meeting/educational-program\ /> Sarah Mack University of Rochester Medical Center Center for Musculoskeletal Research Histology, Biochemistry, and Molecular Imaging Core 601 Elmwood Avenue Box 665 Rochester, NY 14642 (585)-273-3901 From Sarah_Mack <@t> urmc.rochester.edu Thu Feb 21 07:46:24 2013 From: Sarah_Mack <@t> urmc.rochester.edu (Mack, Sarah) Date: Thu Feb 21 07:49:07 2013 Subject: [Histonet] A Call for NYSHS Award Applicants! Message-ID: A Call for NYSHS Award Applicants! The Awards Committee, formed from the offices and board of directors of the society, carefully evaluate all nominees and supporting documentation submitted for the awards. Recipients are presented their award at the New York State Histotechnological Society annual spring meeting. To be eligible, please send: a. A letter from you showing evidence of your commitment to continuing education. b. Two letters of recommendation from supervisor, pathologists, or histotechnologist. c. Name and address of your current employer or school, and your current address. This year?s awards include: Gulf Coast Instrument Company: Two awards are available each $250. Leica Biosystems Award: One at $250. Sakura: One at $250. Source Medical Products: Two awards one at $250 and one at $200. StatLab Medical Products: One at $250. Dominic Europa Award: Will be awarded to a long-standing NYSHS member who serves as an inspiration to others in the histotechnology field. Candidate can be a bench tech or a supervisor. The $500.00 award is sponsored by the NYSHS and must be used to defray educational expenses. All awards must be used to defray educational expenses or to attend a professional meeting. Applications are due March 31st, 2013. We prefer e-mail submissions of applications and letters. Please send documents to: Angela M. Fogg angelafogg@aol.com Sarah Mack University of Rochester Medical Center Center for Musculoskeletal Research Histology, Biochemistry, and Molecular Imaging Core 601 Elmwood Avenue Box 665 Rochester, NY 14642 (585)-273-3901 From Sarah_Mack <@t> urmc.rochester.edu Thu Feb 21 08:19:32 2013 From: Sarah_Mack <@t> urmc.rochester.edu (Mack, Sarah) Date: Thu Feb 21 08:20:15 2013 Subject: [Histonet] SUNY Cobleskill Histotechnology Program Meet and Greet Message-ID: The SUNY Cobleskill Histotechnology is hosting and alumni reunion for all graduates of the SUNY Cobleskill Histotechnology program immediately following the New York State Histotechnological Society Annual Meeting being held on Campus. For more information about the NYSHS annual meeting, please visit: http://www.nyhisto.org/. The reunion will take place in the American Heritage room in Prentice Hall from 5:30-7:00 on Saturday April 13th. Beer, wine and light fare will be provided for a nominal fee of $10.00. Please contact your classmates and colleagues, bring some music and come share time and stories with your peers. A block of rooms is available and reserved at the Best Western Plus Inn of Cobleskill at the meeting convention rate of $77.00 for a single or double room. The reservation deadline is April 1st, 2013. For a reservation call 1-518-234-4321 or email http://www.bestwesterncobleskill.com and give the group code NYSHS. Please RSVP Dr. Pamela Colony at: Colonyp@cobleskill.edu. Payment should be made out to the Alumni Association and mailed to: Alumni Association 211 Knapp Hall, SUNY Cobleskill, Cobleskill, NY 10243 Sarah Mack University of Rochester Medical Center Center for Musculoskeletal Research Histology, Biochemistry, and Molecular Imaging Core 601 Elmwood Avenue Box 665 Rochester, NY 14642 (585)-273-3901 From litepath2000 <@t> yahoo.com Thu Feb 21 08:25:49 2013 From: litepath2000 <@t> yahoo.com (NYSHisto) Date: Thu Feb 21 08:25:53 2013 Subject: [Histonet] SUNY Cobleskill Histotechnology Program Alumni Gathering 4/13/13 Message-ID: <1361456749.63460.YahooMailNeo@web141201.mail.bf1.yahoo.com> SUNY Cobleskill Histotechnology Program Alumni Gathering 4/13/13 The SUNY Cobleskill Histotechnology is hosting and alumni reunion for all graduates of the SUNY Cobleskill Histotechnology program immediately following the New York State Histotechnological Society Annual Meeting being held on the Campus.? For more information about the NYSHS annual meeting, please visit: http://www.nyhisto.org/. The reunion will take place in the American Heritage room in Prentice Hall from 5:30-7:00 on Saturday April 13th. ?Beer, wine and light fare will be provided for a nominal fee of $10.00. Please contact your classmates and colleagues, bring some music and come share time and stories with your peers.? A block of rooms is available and reserved at the Best Western Plus Inn of Cobleskill at the meeting convention rate of $77.00 for a single or double room.? The reservation deadline is April 1st, 2013. ??For a reservation call 1-518-234-4321 or email http://www.bestwesterncobleskill.com and give the group code NYSHS. Please RSVP Dr. ?Pamela Colony at:? Colonyp@cobleskill.edu. Payment should be made out to the Alumni Association and mailed to 211 Knapp Hall, SUNY Cobleskill, Cobleskill, NY 10243 From PMonfils <@t> Lifespan.org Thu Feb 21 09:09:56 2013 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Thu Feb 21 09:12:34 2013 Subject: [Histonet] Storage of formalin fixed tissue In-Reply-To: <5126171F020000BF001D0469@gwiasmtp.uct.ac.za> References: <5126171F020000BF001D0469@gwiasmtp.uct.ac.za> Message-ID: Long term storage in formalin is not usually recommended. After tissues are completely fixed in formalin, 70% ethanol is better for long term storage. From hans <@t> histologistics.com Thu Feb 21 10:13:15 2013 From: hans <@t> histologistics.com (Hans B Snyder) Date: Thu Feb 21 10:13:21 2013 Subject: [Histonet] luciferase use Message-ID: Hello, Does anyone have a luciferase or RFP protocol for labeling cells in vitro? Thank you Hans B Snyder Histologistics 60 Prescott Street Worcester, MA 01605 508-308-7800 hans@histologistics.com From Maxim_71 <@t> mail.ru Thu Feb 21 12:11:35 2013 From: Maxim_71 <@t> mail.ru (Maxim Peshkov) Date: Thu Feb 21 12:14:15 2013 Subject: [Histonet] Storage of formalin fixed tissue Message-ID: <244417519.20130221221135@mail.ru> Lucy, As well 70% EtOH we use 70% IPA for long storage formalin fixed tissues. Maxim Peshkov, Russia, Taganrog. mailto:Maxim_71@mail.ru From sowmyakedarnath <@t> gmail.com Thu Feb 21 12:21:39 2013 From: sowmyakedarnath <@t> gmail.com (Sowmya Kedarnath) Date: Thu Feb 21 12:21:43 2013 Subject: [Histonet] Request for Used-Histotechnology Self Instructional Text Book by Freida Message-ID: Hello Histoneters, Would anyone have a copy of the Histotechnology Self Instructional text book by Freida, for sale? Kindly contact me asap. Best Regards From MICHELLE.LAMPHERE <@t> childrens.com Thu Feb 21 14:01:58 2013 From: MICHELLE.LAMPHERE <@t> childrens.com (Michelle Lamphere) Date: Thu Feb 21 14:02:08 2013 Subject: [Histonet] CAP Gen.55500 Message-ID: Good afternoon We were wondering how other labs handle their training/competencies in response to the revised Competency Assessment question in the Gen Lab portion of the checklist. (GEN.55500) 1. How do you do your training before you start your competency? 2. How do you differentiate initial training from initial competency? 3. Is semiannually after the date of hire? Or after training? Or not until after initial competency? And how does that apply to skills that would only be utilized once or twice a year (such as a special stain that you do not perform on a routine basis but still offer)? 4. How do you train without using actual patients? Example...accessioning, embedding, processing, etc 5. What template do you use to incorporate all six elements that are listed? 6. What are you incorporating as your "test system"? I really appreciate any insight or advice you can offer. Thank you! Michelle M Lamphere, HT (ASCP) Senior Tech, Histology Children's Medical Center 1935 Medical District Drive Dallas, TX 75235 Office :214-456-2798 Histology: 214-456-2318 Fax: 214-456-0779 Please consider the environment before printing this e-mail This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. Please consider the environment before printing this e-mail. This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. From mlunetta <@t> luhcares.org Thu Feb 21 14:20:05 2013 From: mlunetta <@t> luhcares.org (Matthew Lunetta) Date: Thu Feb 21 14:20:13 2013 Subject: [Histonet] 2013 Colorado Society of Histotechnology meeting will be held April 19th & 20th Message-ID: <170CACC1CA297947A168665B532DD05C21AD6AC1@EXLUH01.Ad.luhcares.org> The 2013 Colorado Society of Histotechnology meeting will be held April 19th & 20th at the La Quinta Inn, Fort Collins, CO. The program is now posted on the CSH website. For more information regarding the meeting, accommodations, online registration and payment, please visit http://www.coloradohisto.org/2013/meeting.htm. 2013 CSH Registration Packet: http://www.coloradohisto.org/2013/2013_CSH_Program.pdf Online registration is strongly encouraged. If you have to mail or fax your registration please print legibly and provide a valid email address as receipts are no longer being mailed. If you are interested in attending the meeting please register sooner rather than later. As always, you have up until the day of the meeting to pay. Looking forward to seeing all of you at the meeting in April. Respectfully, Matt Lunetta BS HT(ASCP) Lead Histology Longmont United Hospital From melissa <@t> alliedsearchpartners.com Thu Feb 21 14:21:26 2013 From: melissa <@t> alliedsearchpartners.com (Melissa Phelan) Date: Thu Feb 21 14:21:53 2013 Subject: [Histonet] Unique Job for Technical and Operational Manger in Pathology Message-ID: Hello Histonet, I have a unique opportunity for a Pathologists' Assistant or Histotech with medical office and laboratory management experience along with technical pathology experience in Pennsylvania. Please contact me directly for more details if you fit within this profile. Thank you as always for letting us post our positions on here! Sincerely, To view a complete list of Allied Search Partners current openings go to: http://www.alliedsearchpartners.com/careers.php Melissa Phelan LinkedIn: http://www.linkedin.com/in/melissaphelan President, Laboratory Staffing Allied Search Partners P: 888.388.7571 ext. 102 F: 888.388.7572 www.alliedsearchpartners.com From SSCALISE <@t> beaumont.edu Thu Feb 21 17:11:39 2013 From: SSCALISE <@t> beaumont.edu (Sharon Scalise) Date: Thu Feb 21 17:11:50 2013 Subject: [Histonet] RE: CAP Gen.55500 In-Reply-To: References: Message-ID: <190D98228ADC1747BCE27019B78FD8F30112BD36@EXMail03.ms.beaumont.edu> Another area I am wondering about is competency for the PA's. Anyone have something they can share? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michelle Lamphere Sent: Thursday, February 21, 2013 3:02 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] CAP Gen.55500 Good afternoon We were wondering how other labs handle their training/competencies in response to the revised Competency Assessment question in the Gen Lab portion of the checklist. (GEN.55500) 1. How do you do your training before you start your competency? 2. How do you differentiate initial training from initial competency? 3. Is semiannually after the date of hire? Or after training? Or not until after initial competency? And how does that apply to skills that would only be utilized once or twice a year (such as a special stain that you do not perform on a routine basis but still offer)? 4. How do you train without using actual patients? Example...accessioning, embedding, processing, etc 5. What template do you use to incorporate all six elements that are listed? 6. What are you incorporating as your "test system"? I really appreciate any insight or advice you can offer. Thank you! Michelle M Lamphere, HT (ASCP) Senior Tech, Histology Children's Medical Center 1935 Medical District Drive Dallas, TX 75235 Office :214-456-2798 Histology: 214-456-2318 Fax: 214-456-0779 Please consider the environment before printing this e-mail This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. Please consider the environment before printing this e-mail. This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Thu Feb 21 18:14:21 2013 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Thu Feb 21 18:14:35 2013 Subject: [Histonet] RE: CAP Gen.55500 In-Reply-To: References: Message-ID: <761E2B5697F795489C8710BCC72141FF05660A@ex07.net.ucsf.edu> Michelle, The competencies must be keyed to the persons job description. You hired the person with some kind of assurance they could do the work for a particular job. They them must be trained into your system for that job. 1. How do you do your training before you start your competency? A: The training is driven by a checklist of whatever it is they need to be trained at. You assume they know how to cut sections but you will orient them to the microtome, etc that your lab uses, show them how it works and then have them do it. Once they demonstrate they can do it, you have completed that portion of training and you can also sign off their competency. Then you go down the checklist. It may take many months before they complete the entire training if they are expected to be general histotechs rotating to all sections. 2. How do you differentiate initial training from initial competency? A: Training first, then competency. Even if they "know" how to do it, there will be differences in the way your lab does it verses what they have done elsewhere. The key is for them to know how to do it in your lab and what you expect from them. 3A Is semiannually after the date of hire? Or after training? Or not until after initial competency? A: They are hired, they are trained and at 6 months you review their competency and give a 6-month evaluation. We keep records of all the cases they do in the first 6 months and use that as evidence of competency. You need to determine if they are done training, need more work, etc. After that they are reviewed annually. (so, initial hire, 6 month competency review, then at 18 months they get their first annual review) 3B And how does that apply to skills that would only be utilized once or twice a year (such as a special stain that you do not perform on a routine basis but still offer)? A: there will always be some things that are not done very often. We only include "routine" processes in the initial training and competency. They can be trained in rare tests at the time those come in. After they are trained we require people to do at least one of the rare tests per year, even on a control, to maintain competency. If they do not do that they are taken off the list of people who can do that test and must retrain. 4. How do you train without using actual patients? Example...accessioning, embedding, processing, etc A: all LIS have TEST modules that can also be loaded with dummy patients to use as training modules as well. All other histology training can be done initially on excess tissue saved for that purpose. You can get bx needles and make your own biopsies to train people to embed well. Etc. Make paraffin blocks from excess tissue of various types for initial microtome training. Use controls for special stains. Etc. 5. What template do you use to incorporate all six elements that are listed? A: We have a standard institution-wide template for this. It is up to each area to fill it with the job description that applies and determine how the elements are applied. It is a lot of work initially, but pays off in standardized evaluations later on. 6. What are you incorporating as your "test system"? A: The test system is your standard procedures, but no one actually takes part in the entire system 9ie, tissue procurement to reporting). BUT they must know the elements of each part to understand the whole. So, you can educate them about the parts that take place outside the lab, but they are trained on the parts that take place in your lab that they will actually do. I stress that understanding the entire test system and their part in it is very, very valuable to their understanding of their role. Have "fun!" Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michelle Lamphere Sent: Thursday, February 21, 2013 12:02 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] CAP Gen.55500 Good afternoon We were wondering how other labs handle their training/competencies in response to the revised Competency Assessment question in the Gen Lab portion of the checklist. (GEN.55500) 1. How do you do your training before you start your competency? 2. How do you differentiate initial training from initial competency? 3. Is semiannually after the date of hire? Or after training? Or not until after initial competency? And how does that apply to skills that would only be utilized once or twice a year (such as a special stain that you do not perform on a routine basis but still offer)? 4. How do you train without using actual patients? Example...accessioning, embedding, processing, etc 5. What template do you use to incorporate all six elements that are listed? 6. What are you incorporating as your "test system"? I really appreciate any insight or advice you can offer. Thank you! Michelle M Lamphere, HT (ASCP) Senior Tech, Histology Children's Medical Center 1935 Medical District Drive Dallas, TX 75235 Office :214-456-2798 Histology: 214-456-2318 Fax: 214-456-0779 Please consider the environment before printing this e-mail This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. Please consider the environment before printing this e-mail. This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carl.hobbs <@t> kcl.ac.uk Fri Feb 22 03:23:12 2013 From: carl.hobbs <@t> kcl.ac.uk (Hobbs, Carl) Date: Fri Feb 22 03:25:16 2013 Subject: [Histonet] Re: zebrafish and IHC Message-ID: <00D6B8253EAED840B8D04E23549738182215A0A0@AM2PRD0311MB399.eurprd03.prod.outlook.com> Should be no difference ( no tricks) Have a look here for some images: http://www.immunoportal.com/ Carl Hobbs FIBMS Histology and Imaging Manager Wolfson Centre for Age-Related Diseases School of Biomedical Sciences King's College London Guys Campus London SE1 1UL ? Tel.020 7848 6810 fax 020 7848 6816 carl.hobbs@kcl.ac.uk From relia1 <@t> earthlink.net Fri Feb 22 08:04:42 2013 From: relia1 <@t> earthlink.net (Pam Barker) Date: Fri Feb 22 08:04:54 2013 Subject: [Histonet] RELIA Special Job Alert for Managers - Exciting Management Opportunity in Charlotte, NC Message-ID: <007601ce1105$91ff7630$b5fe6290$@earthlink.net> Hi Histonetters!! I hope you are gearing up for a fun weekend. I have a new job opportunity that I am very excited about and want to share with you. This is one of my best clients. I say this because I have placed a number of people there and they love it! Here is the info: Histology/ Lab Manager - Charlotte, NC A top notch private lab located in Charlotte, NC has asked for RELIA's help in their search for a histology lab manager. This person will be responsible with the day to day operation of a full service histology lab. In addition this person will be leading the continued development and enrichment of the immunohistochemistry department. My client offers an excellent compensation package and a great team to work with. If this is the right job for you RELIA can make it happen! For more information please contact Pam Barker at relia1@earthlink.net or toll free at 866-607-3542 RELIA Solutions is the nation's ONLY recruiting firm specializing in the nationwide permanent placement of histology professionals. To sign up for our free histology careers bulletin please send an e-mail to relia1@earthlink.net and include subscribe in the subject line. Keywords: Histology, histologist, histotechnician, histotechnologist, immunohistochemistry Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From epeters2 <@t> gmu.edu Fri Feb 22 10:56:02 2013 From: epeters2 <@t> gmu.edu (Esther Peters) Date: Fri Feb 22 10:56:10 2013 Subject: [Histonet] Need Microm AP 280-3 embedding center Message-ID: <5127A322.3090806@gmu.edu> The heating element for the front chamber on my formerly very dependable Microm AP 280 embedding center has burned out, right in the middle of teaching histotechniques this semester, and with grad students and me in need of it (of course!). I am wondering if anyone has a working unit of this part (AP 280-3) that might be available? Or if anyone in the Washington, DC, metro area can recommend someone who might be able to fix mine? Thank you! Esther -- Esther C. Peters, Ph.D. Department of Environmental Science & Policy Biology Program, Medical Technology Coordinator George Mason University 4400 University Drive, MSN 5F2 Fairfax, VA 22030 Office: David King Hall 3057 Phone: 703-993-3462 Fax: 703-993-1066 E-mail: epeters2@gmu.edu From Jessica <@t> nsh.org Fri Feb 22 12:05:44 2013 From: Jessica <@t> nsh.org (Jessica Smith) Date: Fri Feb 22 12:12:02 2013 Subject: [Histonet] 2 Day Carolina Symposium Message-ID: <4FC64E7CF6FEC4428E6C6BFC49FAFBA667C9C8@NSH-SRVR01.nsh.local> Register today for the first ever 2 day Carolina Symposium sponsored by the National Society for Histotechnology, the North Carolina Society of Histotechnology and the South Carolina Society of Histology Technicians. This two day collaboration brings the best of the three organizations offering a fantastic educational program and an Exhibit Fair for one low price! Attendees have the opportunity to earn up to 10.5 continuing education credits. HT Eligible? Don't Miss the Full Day HT Readiness Course on April 6th for Only $139.00! Registration Fees Full Symposium (both days) Member: $225 Non-Member: $299 Student Member: $169 One Day Pass Member: $169 Non-Member:$259 HT Readiness Course Only (Saturday, April 6) Member: $139 Non-Member: $139 Download a PDF of the Registration Brochure Register Online Today! Jessica Pellegrini Meeting Coordinator/Social Media Specialist National Society for Histotechnology 8850 Stanford Blvd. Suite 2900 Columbia, MD 21045 Phone: 443-535-4062 Fax:443-535-4055 Jessica@nsh.org | www.nsh.org www.histoconvention.org Follow us online for the latest news/updates! Facebook Twitter Linked In YouTube From BZIMMERM <@t> gru.edu Fri Feb 22 12:41:15 2013 From: BZIMMERM <@t> gru.edu (Zimmerman, Billie) Date: Fri Feb 22 12:41:25 2013 Subject: [Histonet] Competency for Anatomic and Clinical Pathology at Georgia Regents Medical Center (Medical College of GA) Message-ID: <7B3DEB32E69C034EACB479059C5DE3FF756FE6@EX-MLB-03.ad.georgiahealth.edu> I wanted to share our competency spreadsheet that we started at the beginning of the year. I'm attaching the histology version. Not sure if attachments work on the histonet. If not, and you are interested, I'd be glad to share my template with anyone. It addresses all 6 key points. Billie Zimmerman MT(ASCP)QIHC Laboratory Clinical Operations Mgr. BF 117 706-721-5617/3630 Fax: 706-434-4883 Augusta State University and Georgia Health Sciences University have consolidated to become Georgia Regents University. Effective January 9, 2013, my email address has changed to BZIMMERM@gru.edu. Please update your address book to reflect this change. From pathology.histology <@t> gmail.com Fri Feb 22 14:05:28 2013 From: pathology.histology <@t> gmail.com (path lab) Date: Fri Feb 22 14:05:39 2013 Subject: [Histonet] Randomized Curling of Thick (40um) Frozen Sections on Positively Charged Slides Message-ID: Hello Histo-World, We are experiencing randomized curling of our thick (40um) frozen mouse brain sections. This curling occurs in the cortex and hippocampus region (these are coronal sections) during the counterstain process for Cresyl Stain (when the tissue is hydrated post alcohol gradient). Is there any explanation for this? It's not occurring in all of the animals slides and is very random. We have performed this IHC protocol numerous times and never seen this type of artifact before. Thank you for all your help!!! From HParker <@t> Skaggs.Net Fri Feb 22 14:59:10 2013 From: HParker <@t> Skaggs.Net (Parker, Helayne) Date: Fri Feb 22 15:00:00 2013 Subject: [Histonet] Block File Cabinets In-Reply-To: <30436e0f-99a7-40cb-bcee-7b965fa8875f@email1.skaggs.net> References: <30436e0f-99a7-40cb-bcee-7b965fa8875f@email1.skaggs.net> Message-ID: <930EB2E8DF68C544873EDD2A3D5F506007D0300D29@email1.skaggs.net> Hi All, Someone called me from a nearby Histo laboratory. They have approx 75 plastic block file cabinets that they would like to re-home for free, but new owner must pay shipping or come get them in Springfield, MO. If anyone is interested you can contact me at oreillepointers@msn.com and I can put you in contact w/ the appropriate person to speak with. Have a lovely weekend, Helayne Parker, H.T. (ASCP) Pathology Section Head Cox Medical Center Branson P.O. Box 650, Branson, MO 65615 Phone: 417-335-7254 Fax: 417-335-7127 Email: hparker@skaggs.net Web: www.coxhealth.com/branson -------------- next part -------------- CoxHealth ? ranked one of Missouri's Best Hospitals by U.S. News & World Report COXHEALTH CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From BZIMMERM <@t> gru.edu Fri Feb 22 15:20:49 2013 From: BZIMMERM <@t> gru.edu (Zimmerman, Billie) Date: Fri Feb 22 15:22:02 2013 Subject: [Histonet] Antibody IDH1 Message-ID: <7B3DEB32E69C034EACB479059C5DE3FF75726C@EX-MLB-03.ad.georgiahealth.edu> Does anyone perform this antibody?? We can't get it to work on the Ventana Ultra. PhenoPath in Seattle performs it on the Dako autostainer using a small size polymer detection kit. I would appreciate any input. Thanks, Billie Zimmerman Augusta State University and Georgia Health Sciences University have consolidated to become Georgia Regents University. Effective January 9, 2013, my email address has changed to BZIMMERM@gru.edu. Please update your address book to reflect this change. From akbitting <@t> geisinger.edu Fri Feb 22 15:25:30 2013 From: akbitting <@t> geisinger.edu (Bitting, Angela K.) Date: Fri Feb 22 15:25:37 2013 Subject: [Histonet] RE: Antibody IDH1 In-Reply-To: <7B3DEB32E69C034EACB479059C5DE3FF75726C@EX-MLB-03.ad.georgiahealth.edu> References: <7B3DEB32E69C034EACB479059C5DE3FF75726C@EX-MLB-03.ad.georgiahealth.edu> Message-ID: <77F52EFAB8B1694B885E277C48FCD0F63284FDF7@GHSEXMBX4W8K1V.geisinger.edu> Are you using the antibody from Dianova?? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Zimmerman, Billie Sent: Friday, February 22, 2013 4:21 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Antibody IDH1 Does anyone perform this antibody?? We can't get it to work on the Ventana Ultra. PhenoPath in Seattle performs it on the Dako autostainer using a small size polymer detection kit. I would appreciate any input. Thanks, Billie Zimmerman Augusta State University and Georgia Health Sciences University have consolidated to become Georgia Regents University. Effective January 9, 2013, my email address has changed to BZIMMERM@gru.edu. Please update your address book to reflect this change. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. ------------------------------------------------------------------------- This message was secured by ZixCorp(R). From rheyna <@t> lumc.edu Fri Feb 22 15:51:53 2013 From: rheyna <@t> lumc.edu (Roger Heyna) Date: Fri Feb 22 15:53:09 2013 Subject: [Histonet] Antibody IDH1 In-Reply-To: <7B3DEB32E69C034EACB479059C5DE3FF75726C@EX-MLB-03.ad.georgiahealth.edu> References: <7B3DEB32E69C034EACB479059C5DE3FF75726C@EX-MLB-03.ad.georgiahealth.edu> Message-ID: <51279419020000230004AB11@gwgwia1.luhs.org> Billie, We use Dianova's antibody and purchase it from Fisher. We've performed this stain for a little over six months and seem to be having success with the following protocol: Dianova's IDH1 R132H Antibody, Diluted 1:100 Ventana Benchmark XT, iView Detection Standard CC1 37 degrees C 32 minute antibody incubation A/B Block I hope this helps! Roger Heyna Maywood, IL >>> "Zimmerman, Billie" 2/22/2013 3:20 PM >>> Does anyone perform this antibody?? We can't get it to work on the Ventana Ultra. PhenoPath in Seattle performs it on the Dako autostainer using a small size polymer detection kit. I would appreciate any input. Thanks, Billie Zimmerman Augusta State University and Georgia Health Sciences University have consolidated to become Georgia Regents University. Effective January 9, 2013, my email address has changed to BZIMMERM@gru.edu. Please update your address book to reflect this change. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akbitting <@t> geisinger.edu Fri Feb 22 15:59:07 2013 From: akbitting <@t> geisinger.edu (Bitting, Angela K.) Date: Fri Feb 22 15:59:18 2013 Subject: [Histonet] Antibody IDH1 In-Reply-To: <51279419020000230004AB11@gwgwia1.luhs.org> References: <7B3DEB32E69C034EACB479059C5DE3FF75726C@EX-MLB-03.ad.georgiahealth.edu> <51279419020000230004AB11@gwgwia1.luhs.org> Message-ID: <77F52EFAB8B1694B885E277C48FCD0F63284FE3A@GHSEXMBX4W8K1V.geisinger.edu> I received a spec sheet from Dianova that has their Ventana XT protocol. It suggests using CC2 standard retrieval and incubation for 32 minutes with amp. They give a dilution range of 1:20-1:50. It seems to work well for us although we are still validating. I hope that helps. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Roger Heyna Sent: Friday, February 22, 2013 4:52 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Antibody IDH1 Billie, We use Dianova's antibody and purchase it from Fisher. We've performed this stain for a little over six months and seem to be having success with the following protocol: Dianova's IDH1 R132H Antibody, Diluted 1:100 Ventana Benchmark XT, iView Detection Standard CC1 37 degrees C 32 minute antibody incubation A/B Block I hope this helps! Roger Heyna Maywood, IL >>> "Zimmerman, Billie" 2/22/2013 3:20 PM >>> Does anyone perform this antibody?? We can't get it to work on the Ventana Ultra. PhenoPath in Seattle performs it on the Dako autostainer using a small size polymer detection kit. I would appreciate any input. Thanks, Billie Zimmerman Augusta State University and Georgia Health Sciences University have consolidated to become Georgia Regents University. Effective January 9, 2013, my email address has changed to BZIMMERM@gru.edu. Please update your address book to reflect this change. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. ------------------------------------------------------------------------- This message was secured by ZixCorp(R). From Rcartun <@t> harthosp.org Fri Feb 22 15:59:31 2013 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Feb 22 15:59:46 2013 Subject: [Histonet] Antibody IDH1 In-Reply-To: <7B3DEB32E69C034EACB479059C5DE3FF75726C@EX-MLB-03.ad.georgiahealth.edu> References: <7B3DEB32E69C034EACB479059C5DE3FF75726C@EX-MLB-03.ad.georgiahealth.edu> Message-ID: <5127A3F3.7770.0077.1@harthosp.org> We run it on Leica's Bond Max with good results. We use the mouse mAb (Clone H09) from "dianova" at a dilution of 1:50 (15' incubation) following low-pH retrieval. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-2204 Fax >>> "Zimmerman, Billie" 2/22/2013 4:20 PM >>> Does anyone perform this antibody?? We can't get it to work on the Ventana Ultra. PhenoPath in Seattle performs it on the Dako autostainer using a small size polymer detection kit. I would appreciate any input. Thanks, Billie Zimmerman Augusta State University and Georgia Health Sciences University have consolidated to become Georgia Regents University. Effective January 9, 2013, my email address has changed to BZIMMERM@gru.edu. Please update your address book to reflect this change. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TJohnson <@t> gnf.org Fri Feb 22 17:21:34 2013 From: TJohnson <@t> gnf.org (Teri Johnson) Date: Fri Feb 22 17:25:15 2013 Subject: [Histonet] Re: zebrafish and IHC Message-ID: <9F3CFEE76E51B64991C7485270890B402E717C28@EX4.lj.gnf.org> The differences might be due to decalcification and/or differences in fixation. I presume you are decalcifying the fish? Are you using EDTA or a formic acid decalcifier? Are they fixed in 10%NBF (or equivalent) for roughly the same amount of time as your human samples? Or are you using a different fixative altogether. The protocol might need to be re-optimized for your fish samples based on those issues. Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 From meghak <@t> g.clemson.edu Sat Feb 23 04:31:57 2013 From: meghak <@t> g.clemson.edu (Megha Kumar) Date: Sat Feb 23 04:33:53 2013 Subject: [Histonet] sections falling off Message-ID: Hi Histonetters ! Can anyone suggest how to prevent adult mouse skin sections from falling off during in situ hybridization? How to make sections adhere better to slides? I am using poly-lysine coated slides for 5-10 micron thick skin sections. The tissue is fixed in 4% PFA and embedded in paraffin. Please help ! thanks megha -- Megha Kumar, Ph.D. Post-doctoral Research Scholar Sen-Bandyopadhyay Lab Department of Biological Sciences and Bioengineering Indian Institute of Technology Kanpur Kanpur, 208016 India phone - 9695830033 * * * * From carl.hobbs <@t> kcl.ac.uk Sat Feb 23 13:18:49 2013 From: carl.hobbs <@t> kcl.ac.uk (Hobbs, Carl) Date: Sat Feb 23 13:19:04 2013 Subject: [Histonet] Re: zebrafish and IHC Message-ID: <00D6B8253EAED840B8D04E23549738182215A408@AM2PRD0311MB399.eurprd03.prod.outlook.com> Good point by Teri Johnson. I don't decalcify, thus my results may well be compromised by this procedure....and, sure, the bone is disrupted. I am only interested in CNS/PNS so, I don't worry about bone/cartilage disruption. I fix in std 10% NBF. Imho, it is better to "overfix" rather than to worry about optimal short fixation, if you are using HIER. Utilitarily, Carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD School of Biomedical Sciences Kings College London Guys Campus SE1 1UL Tel: 020 78486813 Fax: 020 78486816 020 78486813 From abijag76 <@t> rediffmail.com Sun Feb 24 06:53:48 2013 From: abijag76 <@t> rediffmail.com (abijag ) Date: Sun Feb 24 06:56:47 2013 Subject: [Histonet] Luxol Fast Blue +PAS staining on rat spinal cord Message-ID: <20130224125348.27413.qmail@f4mail-235-121.rediffmail.com> Dear Fello histonetters, I am looking for demonstration of demyelination in rat spinal cord paraffin sections. A quick look into the literature revealed use of combination of luxol fast blue and PAS for demonstration of demyelination and myelin breakdown products. Anybody over here will suggest me the procedure for doing this staining. There is also kit from hitobiotec for this staining. Now my questions are 1. Whats your preferred stain for demonstrating demyleination? 2. Any experience of using LFB+PAS for demonstration of the same 3. Any idea about the performance of this staining kit from hitobiotec Thanks in advance From kdwyer3322 <@t> aol.com Sun Feb 24 10:01:39 2013 From: kdwyer3322 <@t> aol.com (kdwyer3322@aol.com) Date: Sun Feb 24 10:01:42 2013 Subject: [Histonet] Texas Society for Histotechnology 2013 Symposium/Convention Message-ID: <8CFE0BF5CD13C1E-1AD0-69EA9@webmail-d024.sysops.aol.com> All, The Texas Society For Histotechnology meeting is April 12-14, 2013 at the JW. Marriott in Houston, Texas. Deadline to book a hotel room is March 21, 2013. Please join us for excellent educational offerings which includes 17 workshops and 3 symposiums. We hope to see you in Houston, Texas! Please visit txsh.org to view the program. If you have any questions or would like a program e-mailed to you please contact: Kathy Dwyer at kdwyer3322@aol.com From John.Garratt <@t> vch.ca Sun Feb 24 19:30:57 2013 From: John.Garratt <@t> vch.ca (Garratt, John [NS]) Date: Sun Feb 24 19:31:08 2013 Subject: [Histonet] Mismatch Repair Enzymes, do you want to learn more? Message-ID: CIQC/CAP-ACP SEMINAR: DIAGNOSTIC ihc AND MOLECULAR PATHOLOGy This is a unique opportunity to participate in a symposium dedicated to the future of IHC and molecular diagnostics. For registration go to: http://ciqc-capconference.eventbrite.ca/ From tahseen <@t> brain.net.pk Sun Feb 24 23:44:16 2013 From: tahseen <@t> brain.net.pk (tahseen@brain.net.pk) Date: Sun Feb 24 23:44:26 2013 Subject: [Histonet] sections falling off In-Reply-To: References: Message-ID: <10144.203.135.35.66.1361771056.squirrel@brain.net.pk> Dear Megha Kumar, Use commercial + charged OR silanized slides. Silanized slides are prepared by cleaning the slides by washing, followed with a rinse in 95% ethanol. 4ml of 3-aminopropyltriethoxy silane is added to 200 ml of acetone and slides are dipped for 30-60 seconds, followed by 60 seconds in agitated distilled water. The coated slides are then dried for 1 hour, and can be boxed for future use. References:Bancroft sixth edition page 694 Regards Muhammad Tahseen Senior Supervisor Histopathology SKMCH&RC > Hi Histonetters ! > Can anyone suggest how to prevent adult mouse skin sections from falling > off during in situ hybridization? How to make sections adhere better to > slides? I am using poly-lysine coated slides for 5-10 micron thick skin > sections. The tissue is fixed in 4% PFA and embedded in paraffin. > Please help ! > thanks > megha > > > -- > Megha Kumar, Ph.D. > Post-doctoral Research Scholar > Sen-Bandyopadhyay Lab > Department of Biological Sciences and Bioengineering > Indian Institute of Technology Kanpur > Kanpur, 208016 > India > > phone - 9695830033 > > * > * > * > * > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From tgenade <@t> gmail.com Mon Feb 25 01:22:55 2013 From: tgenade <@t> gmail.com (Tyrone Genade) Date: Mon Feb 25 01:28:21 2013 Subject: [Histonet] Re: Histonet Digest, Vol 111, Issue 32 In-Reply-To: <512660d2.89aeec0a.1be4.ffffdae4SMTPIN_ADDED_MISSING@mx.google.com> References: <512660d2.89aeec0a.1be4.ffffdae4SMTPIN_ADDED_MISSING@mx.google.com> Message-ID: Hello, On Thu, Feb 21, 2013 at 8:00 PM, wrote: > From: "Margaryan, Naira" > I am trying to do IHC on FFPE 4 weeks old zebrafish with different Abs. Is there a trick > working with zebrafish? I am using the same IHC protocol I always use on human and > mouse tissue and my Abs are suppose to work on fish as well as human. I run human > and fish sections together with same AR and IHC protocol. By the end I get beautiful > staining on human section and or nothing or some > fuzzy/fuggy/unclarified/undistinguished reaction. I suggest you prepare some unfixed frozen sections for comparison. If the antibody reacts to the unfixed tissue but not the fixed then you probably need to perform one or another antigen retrieval method. I have been having good success with http://www.ncbi.nlm.nih.gov/pubmed/21603650 For my fish, Nothobranchius, I am now fixing for two days at 4 oC (in PFA) and then decalcifying 15% EDTA (pH 7.3) for three days (the last day in 30% sucrose dissolved into the 15% EDTA) before freezing in liquid nitrogen for sectioning. This method is maintaining the structure beautifully. As regards using the above antigen retrieval method I have both soaked the tissue (after fixing) in the 70 oC pH 9 TRIS and then decalcified as well as decalcified, cryo-protected and sectioned (APTES coated slides) and then performed the antigen retrieval at 70 oC. Both routes work well for my fish and the sections don't detach. If you cannot carefully dissect out the tissue of interest then I suggest decalcifying the tissue. You only need one piece of bone to catch on the blade and rip through the tissue to cause a lot of frustration and waste a specimen. I hope this helps. -- Tyrone Genade Ph.D. Department of Human Biology University of Cape Town South Africa http://tgenade.freeshell.org email: tgenade@gmail.com tel: +27-84-632-1925 (c) ******************************************************************************** Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord. To find out how to receive this FREE gift visit http://www.alpha.org. From sforeman <@t> labpath.com Mon Feb 25 06:36:44 2013 From: sforeman <@t> labpath.com (Susan Foreman) Date: Mon Feb 25 06:40:45 2013 Subject: [Histonet] any other company for Ventana service? ULTRA or iSCAN Message-ID: <005d01ce1354$c50c6eb0$4f254c10$@com> Happy Monday, Histonetters, Are you using any company other than Ventana to do Preventative Maintenance or As Needed Service on the Benchmark ULTRA IHC stainer or the iSCAN slide scanner? Our contract is due for renewal and I was curious as to your thoughts? Many Thanks for your input, Susan From CThornton <@t> dahlchase.com Mon Feb 25 09:24:02 2013 From: CThornton <@t> dahlchase.com (Clare Thornton) Date: Mon Feb 25 09:29:34 2013 Subject: [Histonet] streaking when cutting Message-ID: We've had one tech complaining of streaks when cutting, across the paraffin. It's noticeable when cutting, although not clearly noticeable microscopically. This tech has tried everything, tightening and re-tightening of microtome parts, trying other people's chucks or blade holders, new blades, new lots of blades...everything we can think of. It doesn't appear to be the microtome. Last week, I noticed the same streaking occuring on my sections. No one else (we have 9 microtomes total) has complained of this issue. Could this be a paraffin problem? The streaks look like when you might have a bit of paraffin debris on your blade and cut a new section; however, this is occuring with brand new blades, almost every block. Any ideas? Thank you. Clare J. Thornton, HTL(ASCP)QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com From jqb7 <@t> cdc.gov Mon Feb 25 09:31:50 2013 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/OID/NCEZID)) Date: Mon Feb 25 09:32:33 2013 Subject: [Histonet] RE: streaking when cutting In-Reply-To: References: Message-ID: Is the back of the blade holder clear of paraffin debris as well as the blade? Jeanine H. Bartlett Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 404-639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Clare Thornton Sent: Monday, February 25, 2013 10:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] streaking when cutting We've had one tech complaining of streaks when cutting, across the paraffin. It's noticeable when cutting, although not clearly noticeable microscopically. This tech has tried everything, tightening and re-tightening of microtome parts, trying other people's chucks or blade holders, new blades, new lots of blades...everything we can think of. It doesn't appear to be the microtome. Last week, I noticed the same streaking occuring on my sections. No one else (we have 9 microtomes total) has complained of this issue. Could this be a paraffin problem? The streaks look like when you might have a bit of paraffin debris on your blade and cut a new section; however, this is occuring with brand new blades, almost every block. Any ideas? Thank you. Clare J. Thornton, HTL(ASCP)QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Mon Feb 25 09:48:09 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Feb 25 09:48:36 2013 Subject: [Histonet] RE: streaking when cutting In-Reply-To: References: Message-ID: <1361807289.52831.YahooMailNeo@web163105.mail.bf1.yahoo.com> It is probably debris in the paraffin, now if that is the problem why it appears in only 1 or 2 "microtomes"? Because not everybody cuts in the same way, at the same speed and with the same thickness after preparing the block in the same way. These peculiarities in the way you and the other tech section may cause that you both have the streaks and not the other techs. In order to find out if the streaking is due to sectioning personal ways, try to cut a block other tech has cut without complaining about streaks to see if the streaks appear when you cut. Also, if it is caused by debris, the debris will be at the bottom of the block and can be removed during the initial steps until you reach the tissue. If this step is deeper in other techs that what you use to do, they may not experience the problem while you still do. Ren? J. From: "Bartlett, Jeanine (CDC/OID/NCEZID)" To: Clare Thornton ; "histonet@lists.utsouthwestern.edu" Sent: Monday, February 25, 2013 10:31 AM Subject: [Histonet] RE: streaking when cutting Is the back of the blade holder clear of paraffin debris as well as the blade? Jeanine H. Bartlett Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 404-639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Clare Thornton Sent: Monday, February 25, 2013 10:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] streaking when cutting We've had one tech complaining of streaks when cutting, across the paraffin.? It's noticeable when cutting, although not clearly noticeable microscopically.? This tech has tried everything, tightening and re-tightening of microtome parts, trying other people's chucks or blade holders, new blades, new lots of blades...everything we can think of. It doesn't appear to be the microtome.? Last week, I noticed the same streaking occuring on my sections.? No one else (we have 9 microtomes total) has complained of this issue.? Could this be a paraffin problem?? The streaks look like when you might have a bit of paraffin debris on your blade and cut a new section; however, this is occuring with brand new blades, almost every block.? Any ideas?? Thank you. Clare J. Thornton, HTL(ASCP)QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From donna_suresch <@t> merck.com Mon Feb 25 12:07:02 2013 From: donna_suresch <@t> merck.com (Suresch, Donna L.) Date: Mon Feb 25 12:07:07 2013 Subject: [Histonet] Maxvison anti-Rabbit HRP Antibody Substitution Message-ID: <2C9A1D9608959940943F357E0A470FF8B2A9D0BC46@USCTMXP51005.merck.com> Hello All, I have been using Maxvision anti-Rabbit HRP secondary antibody with excellent results. The product has been discontinued. Has anyone found a good substitution for this antibody? Thank you. Donna L. Suresch Senior Imaging Research Scientist Merck Research Laboratories Department of Imaging - West Point Campus Mail Stop: WP44K Office: WP44-H129 770 Sumneytown Pike PO Box 4 West Point, PA 19486-0004 Phone: 215-652-7349 Fax: 215-993-6803 Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From sdysart <@t> mirnarx.com Mon Feb 25 12:15:20 2013 From: sdysart <@t> mirnarx.com (Sarah Dysart) Date: Mon Feb 25 12:19:18 2013 Subject: [Histonet] RE: Maxvison anti-Rabbit HRP Antibody Substitution In-Reply-To: <2C9A1D9608959940943F357E0A470FF8B2A9D0BC46@USCTMXP51005.merck.com> References: <2C9A1D9608959940943F357E0A470FF8B2A9D0BC46@USCTMXP51005.merck.com> Message-ID: <8A70A9B2ECDD084DACFE6C59FCF86D50485058B1@BL2PRD0711MB434.namprd07.prod.outlook.com> The Biocare polymers (MACH2 series) are excellent!! Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Suresch, Donna L. Sent: Monday, February 25, 2013 12:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Maxvison anti-Rabbit HRP Antibody Substitution Hello All, I have been using Maxvision anti-Rabbit HRP secondary antibody with excellent results. The product has been discontinued. Has anyone found a good substitution for this antibody? Thank you. Donna L. Suresch Senior Imaging Research Scientist Merck Research Laboratories Department of Imaging - West Point Campus Mail Stop: WP44K Office: WP44-H129 770 Sumneytown Pike PO Box 4 West Point, PA 19486-0004 Phone: 215-652-7349 Fax: 215-993-6803 Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Valerie.Hannen <@t> parrishmed.com Mon Feb 25 12:21:16 2013 From: Valerie.Hannen <@t> parrishmed.com (Hannen, Valerie) Date: Mon Feb 25 12:21:29 2013 Subject: [Histonet] CAP checklist question Message-ID: <450B7A81EDA0C54E97C53D60F00776C3231B3D7BEC@isexstore03> Hi gang.. I am working on a self-inspection CAP checklist and came across the following: ANP.23120 Tissue Processor Tissue processing schedules are validated. Note: New tissue processing schedules must be validated against the standard laboratory processing schedule. Evidence of compliance: Written procedure for validation of new tissue processing schedules AND WC records documenting validation What do you all make of this?? We have not had any new processing schedules introduced into our lab in years. So, I really don't know how to proceed on this one. Any insite will be greatly appreciated. Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.hannen@parrishmed.com =================== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" =================== From mroark <@t> sfmc.net Mon Feb 25 13:33:55 2013 From: mroark <@t> sfmc.net (Matthew Roark) Date: Mon Feb 25 13:34:03 2013 Subject: [Histonet] Ventana Labels In-Reply-To: <450B7A81EDA0C54E97C53D60F00776C3231B3D7BEC@isexstore03> References: <450B7A81EDA0C54E97C53D60F00776C3231B3D7BEC@isexstore03> Message-ID: <002101ce138f$0cf92a30$26eb7e90$@net> Hello all, I am getting ready to try some slide labels as an alternative to the Ventana labels. I was wondering if anyone else is using them or have tried them in the past. They do not have that plastic fold over flap and are suppose to be quite economical, though I have not got a quote yet. They are called FloProTek (http://www.easternlabsvc.com/floprotek.php) - Mercedes Medical Supply sent me the trial roll. Thanks! Matthew Roark- HT/HTL(ASCP)CM Histology Specialist Saint Francis Medical Center 211 Saint Francis Drive Cape Girardeau, MO 63703 573-331-5267 mroark@sfmc.net http://www.sfmc.net From marktarango <@t> gmail.com Mon Feb 25 13:43:38 2013 From: marktarango <@t> gmail.com (Mark Tarango) Date: Mon Feb 25 13:43:44 2013 Subject: [Histonet] Ventana Labels In-Reply-To: <002101ce138f$0cf92a30$26eb7e90$@net> References: <450B7A81EDA0C54E97C53D60F00776C3231B3D7BEC@isexstore03> <002101ce138f$0cf92a30$26eb7e90$@net> Message-ID: Hi Matthew, We use them and they work fine. They are much cheaper too. We've had them for about 3 years. I initially had some concerns about the DAB being absorbed and staining the labels on some cases. Our safety person told me that it's not a safety concern and it's safe to touch the label after it's stained brown by DAB. This doesn't happen on every slide but is a random thing. It might have something to do with how well it was affixed to the slide. The labels don't affect the staining in my experience. Mark On Mon, Feb 25, 2013 at 11:33 AM, Matthew Roark wrote: > Hello all, > > I am getting ready to try some slide labels as an alternative to the > Ventana > labels. I was wondering if anyone else is using them or have tried them in > the past. > > They do not have that plastic fold over flap and are suppose to be quite > economical, though I have not got a quote yet. > > They are called FloProTek (http://www.easternlabsvc.com/floprotek.php) - > Mercedes Medical Supply sent me the trial roll. > > Thanks! > > > Matthew Roark- HT/HTL(ASCP)CM > Histology Specialist > Saint Francis Medical Center > 211 Saint Francis Drive > Cape Girardeau, MO 63703 > 573-331-5267 > mroark@sfmc.net > http://www.sfmc.net > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From akbitting <@t> geisinger.edu Mon Feb 25 13:55:59 2013 From: akbitting <@t> geisinger.edu (Bitting, Angela K.) Date: Mon Feb 25 13:56:06 2013 Subject: [Histonet] Ventana Labels In-Reply-To: <002101ce138f$0cf92a30$26eb7e90$@net> References: <450B7A81EDA0C54E97C53D60F00776C3231B3D7BEC@isexstore03> <002101ce138f$0cf92a30$26eb7e90$@net> Message-ID: <77F52EFAB8B1694B885E277C48FCD0F6328503A5@GHSEXMBX4W8K1V.geisinger.edu> I just tried them Friday. They worked fine but the label picked up counterstain a bit. However, depending on the cost per label, it may be worth the trouble. I'm waiting for a quote. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Matthew Roark Sent: Monday, February 25, 2013 2:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana Labels Hello all, I am getting ready to try some slide labels as an alternative to the Ventana labels. I was wondering if anyone else is using them or have tried them in the past. They do not have that plastic fold over flap and are suppose to be quite economical, though I have not got a quote yet. They are called FloProTek (http://www.easternlabsvc.com/floprotek.php) - Mercedes Medical Supply sent me the trial roll. Thanks! Matthew Roark- HT/HTL(ASCP)CM Histology Specialist Saint Francis Medical Center 211 Saint Francis Drive Cape Girardeau, MO 63703 573-331-5267 mroark@sfmc.net http://www.sfmc.net _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. 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From sforeman <@t> labpath.com Mon Feb 25 13:57:25 2013 From: sforeman <@t> labpath.com (Susan Foreman) Date: Mon Feb 25 14:01:42 2013 Subject: [Histonet] IgG4 Message-ID: <002e01ce1392$554fa900$ffeefb00$@com> Has anyone worked up IgG4 on the Ventana Benchmark Ultra? What vendor? How about running it on fresh frozen sections? FITC? Vendor? Many Thanks for your input, Susan From nmhisto <@t> comcast.net Mon Feb 25 14:09:08 2013 From: nmhisto <@t> comcast.net (nmhisto@comcast.net) Date: Mon Feb 25 14:09:29 2013 Subject: [Histonet] Streaking when Cutting Message-ID: <1822368949.1502216.1361822948867.JavaMail.root@sz0075a.emeryville.ca.mail.comcast.net> I've been quietly observing from the sidelines, but I just can't pass this one up.? One should never streak when cutting - it's distracting to fellow techs.? Okay - back to my nap. From Allison.Scott <@t> harrishealth.org Mon Feb 25 14:27:55 2013 From: Allison.Scott <@t> harrishealth.org (Scott, Allison D) Date: Mon Feb 25 14:27:59 2013 Subject: [Histonet] Waste Alcohol Message-ID: Hello to all in histoland. Is there anyone putting alcohol into a waste drum like for xylene. We have a company that takes away our xylene. Now the safety people are suggesting that we treat the alcohol the same way. We have always poured the alcohol down the drain. It seems very expensive. Your help in this matter will be greatly appreciated. Allison Scott HT(ASCP) Supervisor, Histology Lab LBJ Hospital Harris Health System Office: 713-566-5287 Lab: 713-566-5287 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. From trathborne <@t> somerset-healthcare.com Mon Feb 25 14:33:27 2013 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Mon Feb 25 14:33:23 2013 Subject: [Histonet] RE: Waste Alcohol In-Reply-To: References: Message-ID: <3AD061FE740D464FAC7BF6B5CFB75707599DF5C3@smcmail02.somerset-healthcare.com> We have been disposing of our alcohols like this for years. This would include alcoholic eosin too. We are allowed to mix the waste xylene with the alcohols because they are the same classification, and treated the same way. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Monday, February 25, 2013 3:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Waste Alcohol Hello to all in histoland. Is there anyone putting alcohol into a waste drum like for xylene. We have a company that takes away our xylene. Now the safety people are suggesting that we treat the alcohol the same way. We have always poured the alcohol down the drain. It seems very expensive. Your help in this matter will be greatly appreciated. Allison Scott HT(ASCP) Supervisor, Histology Lab LBJ Hospital Harris Health System Office: 713-566-5287 Lab: 713-566-5287 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HornHV <@t> archildrens.org Mon Feb 25 15:02:10 2013 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Mon Feb 25 15:02:19 2013 Subject: [Histonet] RE: Waste Alcohol In-Reply-To: References: Message-ID: <25A4DE08332B19499904459F00AAACB719BE135634@EVS1.archildrens.org> Our xylene and alcohol go into a waste drum to be disposed of. We do recycle both but of course we still have waste to dispose of. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Monday, February 25, 2013 2:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Waste Alcohol Hello to all in histoland. Is there anyone putting alcohol into a waste drum like for xylene. We have a company that takes away our xylene. Now the safety people are suggesting that we treat the alcohol the same way. We have always poured the alcohol down the drain. It seems very expensive. Your help in this matter will be greatly appreciated. Allison Scott HT(ASCP) Supervisor, Histology Lab LBJ Hospital Harris Health System Office: 713-566-5287 Lab: 713-566-5287 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From mpence <@t> grhs.net Mon Feb 25 15:18:12 2013 From: mpence <@t> grhs.net (Mike Pence) Date: Mon Feb 25 15:19:43 2013 Subject: [Histonet] RE: Waste Alcohol In-Reply-To: References: Message-ID: You should be recycling both xylene and alcohol. Cost savings compared to disposal cost will pay for a recycler in 3-5 years. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Scott, Allison D [Allison.Scott@harrishealth.org] Sent: Monday, February 25, 2013 2:27 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Waste Alcohol Hello to all in histoland. Is there anyone putting alcohol into a waste drum like for xylene. We have a company that takes away our xylene. Now the safety people are suggesting that we treat the alcohol the same way. We have always poured the alcohol down the drain. It seems very expensive. Your help in this matter will be greatly appreciated. Allison Scott HT(ASCP) Supervisor, Histology Lab LBJ Hospital Harris Health System Office: 713-566-5287 Lab: 713-566-5287 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Mon Feb 25 15:53:22 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Feb 25 15:53:26 2013 Subject: [Histonet] Waste Alcohol In-Reply-To: References: Message-ID: <1361829202.27805.YahooMailNeo@web163104.mail.bf1.yahoo.com> I always dumped the ethanol down the drain. I never recycled it because it takes almost 3 times more time that recycling xylene (3 times the cost) and the recycled alcohol is seldom of good quality. When I stopped using xylene I had no more use (and exposure) when recycling. Ren? J. From: "Scott, Allison D" To: "histonet@lists.utsouthwestern.edu" Sent: Monday, February 25, 2013 3:27 PM Subject: [Histonet] Waste Alcohol Hello to all in histoland.? Is there anyone putting alcohol into a waste drum like for xylene.? We have a company that takes away our xylene.? Now the safety people are suggesting that we treat the alcohol the same way.? We have always poured the alcohol down the drain.? It seems very expensive.? Your help in this matter will be greatly appreciated. Allison Scott HT(ASCP) Supervisor, Histology Lab LBJ Hospital Harris Health System Office: 713-566-5287 Lab: 713-566-5287 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged.? This e-mail may also be confidential and/or privileged under Texas law.? The e-mail is for the use of only the individual or entity named above.? If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From karla <@t> dermatopathologynorthwest.com Mon Feb 25 15:47:34 2013 From: karla <@t> dermatopathologynorthwest.com (Karla J. C.) Date: Mon Feb 25 15:56:05 2013 Subject: [Histonet] Dako autostainer service Message-ID: <000f01ce13a1$b8de93a0$2a9bbae0$@dermatopathologynorthwest.com> Hi All, Help.Dako just recently informed us that they would no longer provide a Service contract for our immuno autostainer. Is anyone else experiencing this? Does anyone know of any other service people that may be able to work on this equipment (model 3400 year 2003)? We are located in Seattle. Thanks, Karla Carlmas Dermatopathology Northwest From Susan.Walzer <@t> HCAHealthcare.com Tue Feb 26 01:55:58 2013 From: Susan.Walzer <@t> HCAHealthcare.com (Susan.Walzer@HCAHealthcare.com) Date: Tue Feb 26 02:02:58 2013 Subject: [Histonet] RE: CAP checklist question In-Reply-To: <450B7A81EDA0C54E97C53D60F00776C3231B3D7BEC@isexstore03> References: <450B7A81EDA0C54E97C53D60F00776C3231B3D7BEC@isexstore03> Message-ID: <4BF03F5404EBDE409AF9232DA74B9DED2DFF10BF41@FWDCWPMSGCMS09.hca.corpad.net> We put in a procedure stating that should we change the schedule we will test a variety of tissue (10 different types) and have the pathologist assess the run. We have not changed a schedule in years but we seek to comply... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie Sent: Monday, February 25, 2013 1:21 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP checklist question Hi gang.. I am working on a self-inspection CAP checklist and came across the following: ANP.23120 Tissue Processor Tissue processing schedules are validated. Note: New tissue processing schedules must be validated against the standard laboratory processing schedule. Evidence of compliance: Written procedure for validation of new tissue processing schedules AND WC records documenting validation What do you all make of this?? We have not had any new processing schedules introduced into our lab in years. So, I really don't know how to proceed on this one. Any insite will be greatly appreciated. Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.hannen@parrishmed.com ============= "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ============= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From portera <@t> msu.edu Tue Feb 26 08:14:28 2013 From: portera <@t> msu.edu (Amy Porter) Date: Tue Feb 26 08:14:33 2013 Subject: [Histonet] Question for Alkaline Phosphatase / Biotin System Users Message-ID: <000d01ce142b$96d73ac0$c485b040$@edu> We are using standard avidin / biotin staining with alkaline phosphatase labeling and sometimes, not every time and not every slide in the same run we get residual blotchy spots and streaking of the reaction on and off the tissue. We have increased to two rinses after primary and two rinses after alk phos reagent prior to substrate; still happening on some slides...anyone else have this experience or type of problem? Any experiences or suggestions are appreciated. Just can't figure out what is causing it.. Amy S. Porter, HT(ASCP) QIHC Michigan State University Investigative HistoPathology Laboratory portera@msu.edu From HornHV <@t> archildrens.org Tue Feb 26 09:27:03 2013 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Tue Feb 26 09:27:40 2013 Subject: [Histonet] Waste Alcohol In-Reply-To: <1361829202.27805.YahooMailNeo@web163104.mail.bf1.yahoo.com> References: <1361829202.27805.YahooMailNeo@web163104.mail.bf1.yahoo.com> Message-ID: <25A4DE08332B19499904459F00AAACB719BE13563A@EVS1.archildrens.org> The ability to put alcohol down the drain is regulated by your wastewater utility. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Monday, February 25, 2013 3:53 PM To: Scott, Allison D; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Waste Alcohol I always dumped the ethanol down the drain. I never recycled it because it takes almost 3 times more time that recycling xylene (3 times the cost) and the recycled alcohol is seldom of good quality. When I stopped using xylene I had no more use (and exposure) when recycling. Ren? J. From: "Scott, Allison D" To: "histonet@lists.utsouthwestern.edu" Sent: Monday, February 25, 2013 3:27 PM Subject: [Histonet] Waste Alcohol Hello to all in histoland.? Is there anyone putting alcohol into a waste drum like for xylene.? We have a company that takes away our xylene.? Now the safety people are suggesting that we treat the alcohol the same way.? We have always poured the alcohol down the drain.? It seems very expensive.? Your help in this matter will be greatly appreciated. Allison Scott HT(ASCP) Supervisor, Histology Lab LBJ Hospital Harris Health System Office: 713-566-5287 Lab: 713-566-5287 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged.? This e-mail may also be confidential and/or privileged under Texas law.? The e-mail is for the use of only the individual or entity named above.? If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From tpodawiltz <@t> lrgh.org Tue Feb 26 09:55:14 2013 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Tue Feb 26 09:56:22 2013 Subject: [Histonet] Waste Alcohol In-Reply-To: <25A4DE08332B19499904459F00AAACB719BE13563A@EVS1.archildrens.org> References: <1361829202.27805.YahooMailNeo@web163104.mail.bf1.yahoo.com> <25A4DE08332B19499904459F00AAACB719BE13563A@EVS1.archildrens.org> Message-ID: <38667E7FB77ECD4E91BFAEB8D986386324FCF3C6D9@LRGHEXVS1.practice.lrgh.org> Reagent Alcohol contains methanol, which is a U-listed EPA hazardous chemical and cannot be poured down the drain. Tom Podawiltz HT (ASCP) Histology Section Head/Laboratory Safety Officer. LRGHealthcare Laconia, NH 03246 603-524-3211 ext: 3220 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Tuesday, February 26, 2013 10:27 AM To: 'Rene J Buesa'; Scott, Allison D; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Waste Alcohol The ability to put alcohol down the drain is regulated by your wastewater utility. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Monday, February 25, 2013 3:53 PM To: Scott, Allison D; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Waste Alcohol I always dumped the ethanol down the drain. I never recycled it because it takes almost 3 times more time that recycling xylene (3 times the cost) and the recycled alcohol is seldom of good quality. When I stopped using xylene I had no more use (and exposure) when recycling. Ren? J. From: "Scott, Allison D" To: "histonet@lists.utsouthwestern.edu" Sent: Monday, February 25, 2013 3:27 PM Subject: [Histonet] Waste Alcohol Hello to all in histoland.? Is there anyone putting alcohol into a waste drum like for xylene.? We have a company that takes away our xylene.? Now the safety people are suggesting that we treat the alcohol the same way.? We have always poured the alcohol down the drain.? It seems very expensive.? Your help in this matter will be greatly appreciated. Allison Scott HT(ASCP) Supervisor, Histology Lab LBJ Hospital Harris Health System Office: 713-566-5287 Lab: 713-566-5287 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged.? This e-mail may also be confidential and/or privileged under Texas law.? The e-mail is for the use of only the individual or entity named above.? If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From brendal.finlay <@t> medicalcenterclinic.com Tue Feb 26 09:56:12 2013 From: brendal.finlay <@t> medicalcenterclinic.com (Brendal Finlay) Date: Tue Feb 26 09:56:25 2013 Subject: [Histonet] Waste Alcohol Message-ID: <4fcdbb6eed8fb2fc1e5ea8e615ce00ee@medicalcenterclinic.com> You?will probably want to contact your local or state office of environmental protection to find out what is required in your area.? ? The EPA lists hazardous wastes in 40 CFR 261.30.? Even if your waste is not on this list, it may have hazardous characteristics such as: ignitability, corrosivity, reactivity, and toxicity characteristic.? Ignitability pertains to flashpoints less than 140 F and/or aqueous solutions with alcohol content greater than or equal to 24 percent.? ? Florida's DEP has different regulations dependent upon the quantity of hazardous waste generated.? Some localities may require approval to dispose of alcohol down the drain.? If you don't have this approval, you have to dispose of the waste according to?your state?and?federalregulations.? ? To reduce the quantity of hazardous waste we generate, we began to recycle using CBG Biotech's distillation unit.? We have had great results with it.? While alcohol does take longer to recycle than xylene, our supply costs have been greatly reduced and we have worked this into our weekly schedule very well. ? Some things to consider when using recycled alcohol: Test each finished alcohol run with a hydrometer to know what percentage it is.? Do not use recycled alcohol at the end of the stain line after eosin (it will wash the eosin out). Do not use recycled alcohol as the last alcohol before xylene on the processor. Finally, do not recycle the first alcohols on the stain line after xylene (at least on our recycler). This is disposed of in our flammable waste containers to be picked up. ? Here?s a link to the EPA?s managing hazardous waste pdf - http://www.epa.gov/osw/hazard/generation/sqg/handbook/k01005.pdf Brendal Finlay, HT (ASCP) http://medicalcenterclinic.com -----Original message----- From: Rene J Buesa rjbuesa@yahoo.com Date: Mon, 25 Feb 201316:53:22 -0600 To: "Scott, Allison D" Allison.Scott@harrishealth.org, "histonet@lists.utsouthwestern.edu" histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Waste Alcohol I always dumped the ethanol down the drain. I never recycled it because it takes almost 3 times more time that recycling xylene (3 times the cost) and the recycled alcohol is seldom of good quality. When I stopped using xylene I had no more use (and exposure) when recycling. Ren? J. From: "Scott, Allison D" To: "histonet@lists.utsouthwestern.edu" Sent: Monday, February 25, 2013 3:27 PM Subject: [Histonet] Waste Alcohol Hello to all in histoland.? Is there anyone putting alcohol into a waste drum like for xylene.? We have a company that takes away our xylene.? Now the safety people are suggesting that we treat the alcohol thesame way.? We have always poured the alcohol down the drain.? It seems very expensive.? Your help in this matter will be greatly appreciated. Allison Scott HT(ASCP) Supervisor, Histology Lab LBJ Hospital Harris Health System Office: 713-566-5287 Lab: 713-566-5287 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged.? This e-mail may also be confidential and/or privileged under Texas law.? The e-mail is forthe use of only the individual or entity named above.? If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From fourfonners <@t> yahoo.com Tue Feb 26 11:33:37 2013 From: fourfonners <@t> yahoo.com (Sheila Fonner) Date: Tue Feb 26 11:33:45 2013 Subject: [Histonet] streaks Message-ID: <1361900017.78056.YahooMailNeo@web140605.mail.bf1.yahoo.com> Hi Claire, ? I may be grasping at straws here, but I went to an NSH workshop once where this very question was posed.? You won't believe what the cause was.? They had a bad lot of blades.? The whole lot was nicked, so changing to a new knife or a new box of knives did not help the problem.? Changing the lot did the trick.? I don't know if this is your problem or not, but I thought it was very interesting. ? Sheila HT (ASCP) Knoxville, TN From Robert.Fauck <@t> ccdhb.org.nz Tue Feb 26 13:28:21 2013 From: Robert.Fauck <@t> ccdhb.org.nz (Robert Fauck [CCDHB]) Date: Tue Feb 26 13:31:24 2013 Subject: [Histonet] Bob.1 SP92 Message-ID: <9C92DFB6EA9983408AA9785182E9FC96680481@WN0NTEML11.AD.CCDHB.HEALTH.NZ> Hi All I have a question about Bob.1 SP92 antibody, what is approximately the optimal titre for this AB from Cell Marque? Thanking you all for your suggestions. Robert Fauck Wellington Hospital, NZ This email or attachment(s) may contain confidential or legally privileged information intended for the sole use of the addressee(s). Any use, redistribution, disclosure, or reproduction of this message, except as intended, is prohibited. If you received this email in error, please notify the sender and remove all copies of the message, including any attachments. Any views or opinions expressed in this email (unless otherwise stated) may not represent those of Capital & Coast District Health Board. http://www.ccdhb.org.nz (1C_S1) From christen <@t> vet.k-state.edu Tue Feb 26 15:11:21 2013 From: christen <@t> vet.k-state.edu (Shelly Christenson) Date: Tue Feb 26 15:11:28 2013 Subject: [Histonet] Gram stain on decalcified bone Message-ID: <06E342B6098ED9478347E1407764C80407C6DC53@VETMXHT.ads.vet.k-state.edu> I have a pathologist that wants a gram stain on decalcified bone. We are currently using the Twort's gram stain and the stain did not stain the bacteria in the bone sample, but the control tissue did stain positive. I was wondering if the acid in the decal solution could have affected the bacteria in the bone sample. I'm not for sure how long the bone was decaled, the H&E doesn't look like it was over decaled. Does anyone know of a gram stain that will work on decalcified bone? Thank you for your help, Shelly Christenson HT(ASCP) Veterinary Diagnostic Lab Mosier Hall L-216 Kansas State University Manhattan, Kansas 66506 785/532-4464 christen@vet.k-state.edu From SHUNTER <@t> beaumont.edu Tue Feb 26 15:53:21 2013 From: SHUNTER <@t> beaumont.edu (Sue Hunter) Date: Tue Feb 26 15:54:38 2013 Subject: [Histonet] IgG4 In-Reply-To: <002e01ce1392$554fa900$ffeefb00$@com> References: <002e01ce1392$554fa900$ffeefb00$@com> Message-ID: We use Cell Marques Mouse monoclonal (clone MRQ-44) Cat# 367M-16 at a 1:200 dilution on the Ultra. We are using the Optiview detection kits. CC#1-24 min Option 5 - 12 min Antibody - 24 min OV Linker 8 min Multimer 8 min H2O2 +DAB 8 min OV copper 4 min Hematozylin II 8min bluing 4 min We only test FFPE tissues. Sue Sue Hunter, Supervisor Advanced Diagnostics Beaumont Health System Royal Oak MI 248-898-5146 shunter@beaumont.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Susan Foreman Sent: Monday, February 25, 2013 2:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IgG4 Has anyone worked up IgG4 on the Ventana Benchmark Ultra? What vendor? How about running it on fresh frozen sections? FITC? Vendor? Many Thanks for your input, Susan _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Erik.Dokken <@t> onassignment.com Tue Feb 26 15:53:36 2013 From: Erik.Dokken <@t> onassignment.com (Erik Dokken) Date: Tue Feb 26 15:54:42 2013 Subject: [Histonet] RE: Histonet Digest, Vol 111, Issue 37 In-Reply-To: <201302261736.r1QHVq5G025624@smtp12.onasgn.net> References: <201302261736.r1QHVq5G025624@smtp12.onasgn.net> Message-ID: <23BE4C143E07A64EBF7E861F61BC5929659391DD@oasslcexm03.oaifield.onasgn.com> Good afternoon Histonetters! We are currently looking for a qualified Pathology Assistant (ASCP Cert Required) for a full time position with a lab in Palo Alto, CA. Job description below; The Pathologists' Assistant (PA) dissects tissue specimens and provides dictated placement in plastic cassettes for embedding, staining, and microscopic analysis. This position directs coding of specimens for the accessioning staff. Processes breast and other tissue specimens according to established protocols, and instructs residents, fellows, medical students, and faculty in Gross Room protocols and processes as well as specimen photography. This position is responsible for responding on a STAT basis to the frozen section room adjacent to the SHC operating rooms, and performs intraoperative frozen sections. The PA is also responsible for assembling catalogues and preserving specimens for the gross specimen teaching library, and the Department of Pathology Tissue Bank. This position prepares and performs specimen radiography of breast, bone, and soft tissue specimens. ASCP certification as a Pathology Assistant required. If you know of anyone looking for work in the Bay Area we would greatly appreciate the referral. Thank you again, Erik Dokken Erik.dokken@onassignment.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Tuesday, February 26, 2013 9:36 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 111, Issue 37 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Maxvison anti-Rabbit HRP Antibody Substitution (Suresch, Donna L.) 2. RE: Maxvison anti-Rabbit HRP Antibody Substitution (Sarah Dysart) 3. CAP checklist question (Hannen, Valerie) 4. Ventana Labels (Matthew Roark) 5. Re: Ventana Labels (Mark Tarango) 6. RE: Ventana Labels (Bitting, Angela K.) 7. IgG4 (Susan Foreman) 8. Streaking when Cutting (nmhisto@comcast.net) 9. Waste Alcohol (Scott, Allison D) 10. RE: Waste Alcohol (Rathborne, Toni) 11. RE: Waste Alcohol (Horn, Hazel V) 12. RE: Waste Alcohol (Mike Pence) 13. Re: Waste Alcohol (Rene J Buesa) 14. Dako autostainer service (Karla J. C.) 15. RE: CAP checklist question (Susan.Walzer@HCAHealthcare.com) 16. Question for Alkaline Phosphatase / Biotin System Users (Amy Porter) 17. RE: Waste Alcohol (Horn, Hazel V) 18. RE: Waste Alcohol (Podawiltz, Thomas) 19. Re: Waste Alcohol (Brendal Finlay) 20. streaks (Sheila Fonner) ---------------------------------------------------------------------- Message: 1 Date: Mon, 25 Feb 2013 13:07:02 -0500 From: "Suresch, Donna L." Subject: [Histonet] Maxvison anti-Rabbit HRP Antibody Substitution To: "histonet@lists.utsouthwestern.edu" Message-ID: <2C9A1D9608959940943F357E0A470FF8B2A9D0BC46@USCTMXP51005.merck.com> Content-Type: text/plain; charset="us-ascii" Hello All, I have been using Maxvision anti-Rabbit HRP secondary antibody with excellent results. The product has been discontinued. Has anyone found a good substitution for this antibody? Thank you. Donna L. Suresch Senior Imaging Research Scientist Merck Research Laboratories Department of Imaging - West Point Campus Mail Stop: WP44K Office: WP44-H129 770 Sumneytown Pike PO Box 4 West Point, PA 19486-0004 Phone: 215-652-7349 Fax: 215-993-6803 Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------ Message: 2 Date: Mon, 25 Feb 2013 18:15:20 +0000 From: Sarah Dysart Subject: [Histonet] RE: Maxvison anti-Rabbit HRP Antibody Substitution To: "Suresch, Donna L." , "histonet@lists.utsouthwestern.edu" Message-ID: <8A70A9B2ECDD084DACFE6C59FCF86D50485058B1@BL2PRD0711MB434.namprd07.prod.outlook.com> Content-Type: text/plain; charset="us-ascii" The Biocare polymers (MACH2 series) are excellent!! Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Suresch, Donna L. Sent: Monday, February 25, 2013 12:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Maxvison anti-Rabbit HRP Antibody Substitution Hello All, I have been using Maxvision anti-Rabbit HRP secondary antibody with excellent results. The product has been discontinued. Has anyone found a good substitution for this antibody? Thank you. Donna L. Suresch Senior Imaging Research Scientist Merck Research Laboratories Department of Imaging - West Point Campus Mail Stop: WP44K Office: WP44-H129 770 Sumneytown Pike PO Box 4 West Point, PA 19486-0004 Phone: 215-652-7349 Fax: 215-993-6803 Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Mon, 25 Feb 2013 13:21:16 -0500 From: "Hannen, Valerie" Subject: [Histonet] CAP checklist question To: "histonet@lists.utsouthwestern.edu" Message-ID: <450B7A81EDA0C54E97C53D60F00776C3231B3D7BEC@isexstore03> Content-Type: text/plain; charset="us-ascii" Hi gang.. I am working on a self-inspection CAP checklist and came across the following: ANP.23120 Tissue Processor Tissue processing schedules are validated. Note: New tissue processing schedules must be validated against the standard laboratory processing schedule. Evidence of compliance: Written procedure for validation of new tissue processing schedules AND WC records documenting validation What do you all make of this?? We have not had any new processing schedules introduced into our lab in years. So, I really don't know how to proceed on this one. Any insite will be greatly appreciated. Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.hannen@parrishmed.com =================== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" =================== ------------------------------ Message: 4 Date: Mon, 25 Feb 2013 13:33:55 -0600 From: "Matthew Roark" Subject: [Histonet] Ventana Labels To: Message-ID: <002101ce138f$0cf92a30$26eb7e90$@net> Content-Type: text/plain; charset="us-ascii" Hello all, I am getting ready to try some slide labels as an alternative to the Ventana labels. I was wondering if anyone else is using them or have tried them in the past. They do not have that plastic fold over flap and are suppose to be quite economical, though I have not got a quote yet. They are called FloProTek (http://www.easternlabsvc.com/floprotek.php) - Mercedes Medical Supply sent me the trial roll. Thanks! Matthew Roark- HT/HTL(ASCP)CM Histology Specialist Saint Francis Medical Center 211 Saint Francis Drive Cape Girardeau, MO 63703 573-331-5267 mroark@sfmc.net http://www.sfmc.net ------------------------------ Message: 5 Date: Mon, 25 Feb 2013 11:43:38 -0800 From: Mark Tarango Subject: Re: [Histonet] Ventana Labels To: Matthew Roark Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hi Matthew, We use them and they work fine. They are much cheaper too. We've had them for about 3 years. I initially had some concerns about the DAB being absorbed and staining the labels on some cases. Our safety person told me that it's not a safety concern and it's safe to touch the label after it's stained brown by DAB. This doesn't happen on every slide but is a random thing. It might have something to do with how well it was affixed to the slide. The labels don't affect the staining in my experience. Mark On Mon, Feb 25, 2013 at 11:33 AM, Matthew Roark wrote: > Hello all, > > I am getting ready to try some slide labels as an alternative to the > Ventana labels. I was wondering if anyone else is using them or have > tried them in the past. > > They do not have that plastic fold over flap and are suppose to be > quite economical, though I have not got a quote yet. > > They are called FloProTek (http://www.easternlabsvc.com/floprotek.php) > - Mercedes Medical Supply sent me the trial roll. > > Thanks! > > > Matthew Roark- HT/HTL(ASCP)CM > Histology Specialist > Saint Francis Medical Center > 211 Saint Francis Drive > Cape Girardeau, MO 63703 > 573-331-5267 > mroark@sfmc.net > http://www.sfmc.net > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 6 Date: Mon, 25 Feb 2013 19:55:59 +0000 From: "Bitting, Angela K." Subject: RE: [Histonet] Ventana Labels To: Matthew Roark , "histonet@lists.utsouthwestern.edu" Message-ID: <77F52EFAB8B1694B885E277C48FCD0F6328503A5@GHSEXMBX4W8K1V.geisinger.edu> Content-Type: text/plain; charset="us-ascii" I just tried them Friday. They worked fine but the label picked up counterstain a bit. However, depending on the cost per label, it may be worth the trouble. I'm waiting for a quote. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Matthew Roark Sent: Monday, February 25, 2013 2:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana Labels Hello all, I am getting ready to try some slide labels as an alternative to the Ventana labels. I was wondering if anyone else is using them or have tried them in the past. They do not have that plastic fold over flap and are suppose to be quite economical, though I have not got a quote yet. They are called FloProTek (http://www.easternlabsvc.com/floprotek.php) - Mercedes Medical Supply sent me the trial roll. Thanks! Matthew Roark- HT/HTL(ASCP)CM Histology Specialist Saint Francis Medical Center 211 Saint Francis Drive Cape Girardeau, MO 63703 573-331-5267 mroark@sfmc.net http://www.sfmc.net _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. ------------------------------------------------------------------------- This message was secured by ZixCorp(R). ------------------------------ Message: 7 Date: Mon, 25 Feb 2013 14:57:25 -0500 From: "Susan Foreman" Subject: [Histonet] IgG4 To: Message-ID: <002e01ce1392$554fa900$ffeefb00$@com> Content-Type: text/plain; charset="us-ascii" Has anyone worked up IgG4 on the Ventana Benchmark Ultra? What vendor? How about running it on fresh frozen sections? FITC? Vendor? Many Thanks for your input, Susan ------------------------------ Message: 8 Date: Mon, 25 Feb 2013 20:09:08 +0000 (UTC) From: nmhisto@comcast.net Subject: [Histonet] Streaking when Cutting To: HISTONET Message-ID: <1822368949.1502216.1361822948867.JavaMail.root@sz0075a.emeryville.ca.mail.comcast.net> Content-Type: text/plain; charset=utf-8 I've been quietly observing from the sidelines, but I just can't pass this one up.?? One should never streak when cutting - it's distracting to fellow techs.?? Okay - back to my nap. ------------------------------ Message: 9 Date: Mon, 25 Feb 2013 20:27:55 +0000 From: "Scott, Allison D" Subject: [Histonet] Waste Alcohol To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Hello to all in histoland. Is there anyone putting alcohol into a waste drum like for xylene. We have a company that takes away our xylene. Now the safety people are suggesting that we treat the alcohol the same way. We have always poured the alcohol down the drain. It seems very expensive. Your help in this matter will be greatly appreciated. Allison Scott HT(ASCP) Supervisor, Histology Lab LBJ Hospital Harris Health System Office: 713-566-5287 Lab: 713-566-5287 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. ------------------------------ Message: 10 Date: Mon, 25 Feb 2013 20:33:27 +0000 From: "Rathborne, Toni" Subject: [Histonet] RE: Waste Alcohol To: "'Scott, Allison D'" , "histonet@lists.utsouthwestern.edu" Message-ID: <3AD061FE740D464FAC7BF6B5CFB75707599DF5C3@smcmail02.somerset-healthcare.com> Content-Type: text/plain; charset="us-ascii" We have been disposing of our alcohols like this for years. This would include alcoholic eosin too. We are allowed to mix the waste xylene with the alcohols because they are the same classification, and treated the same way. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Monday, February 25, 2013 3:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Waste Alcohol Hello to all in histoland. Is there anyone putting alcohol into a waste drum like for xylene. We have a company that takes away our xylene. Now the safety people are suggesting that we treat the alcohol the same way. We have always poured the alcohol down the drain. It seems very expensive. Your help in this matter will be greatly appreciated. Allison Scott HT(ASCP) Supervisor, Histology Lab LBJ Hospital Harris Health System Office: 713-566-5287 Lab: 713-566-5287 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Mon, 25 Feb 2013 15:02:10 -0600 From: "Horn, Hazel V" Subject: [Histonet] RE: Waste Alcohol To: "'Scott, Allison D'" , "histonet@lists.utsouthwestern.edu" Message-ID: <25A4DE08332B19499904459F00AAACB719BE135634@EVS1.archildrens.org> Content-Type: text/plain; charset="us-ascii" Our xylene and alcohol go into a waste drum to be disposed of. We do recycle both but of course we still have waste to dispose of. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Monday, February 25, 2013 2:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Waste Alcohol Hello to all in histoland. Is there anyone putting alcohol into a waste drum like for xylene. We have a company that takes away our xylene. Now the safety people are suggesting that we treat the alcohol the same way. We have always poured the alcohol down the drain. It seems very expensive. Your help in this matter will be greatly appreciated. Allison Scott HT(ASCP) Supervisor, Histology Lab LBJ Hospital Harris Health System Office: 713-566-5287 Lab: 713-566-5287 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ------------------------------ Message: 12 Date: Mon, 25 Feb 2013 21:18:12 +0000 From: Mike Pence Subject: [Histonet] RE: Waste Alcohol To: "Scott, Allison D" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" You should be recycling both xylene and alcohol. Cost savings compared to disposal cost will pay for a recycler in 3-5 years. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Scott, Allison D [Allison.Scott@harrishealth.org] Sent: Monday, February 25, 2013 2:27 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Waste Alcohol Hello to all in histoland. Is there anyone putting alcohol into a waste drum like for xylene. We have a company that takes away our xylene. Now the safety people are suggesting that we treat the alcohol the same way. We have always poured the alcohol down the drain. It seems very expensive. Your help in this matter will be greatly appreciated. Allison Scott HT(ASCP) Supervisor, Histology Lab LBJ Hospital Harris Health System Office: 713-566-5287 Lab: 713-566-5287 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 13 Date: Mon, 25 Feb 2013 13:53:22 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] Waste Alcohol To: "Scott, Allison D" , "histonet@lists.utsouthwestern.edu" Message-ID: <1361829202.27805.YahooMailNeo@web163104.mail.bf1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I always dumped the ethanol down the drain. I never recycled it because it takes almost 3 times more time that recycling xylene (3 times the cost) and the recycled alcohol is seldom of good quality. When I stopped using xylene I had no more use (and exposure) when recycling. Ren? J. From: "Scott, Allison D" To: "histonet@lists.utsouthwestern.edu" Sent: Monday, February 25, 2013 3:27 PM Subject: [Histonet] Waste Alcohol Hello to all in histoland.? Is there anyone putting alcohol into a waste drum like for xylene.? We have a company that takes away our xylene.? Now the safety people are suggesting that we treat the alcohol the same way.? We have always poured the alcohol down the drain.? It seems very expensive.? Your help in this matter will be greatly appreciated. Allison Scott HT(ASCP) Supervisor, Histology Lab LBJ Hospital Harris Health System Office: 713-566-5287 Lab: 713-566-5287 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged.? This e-mail may also be confidential and/or privileged under Texas law.? The e-mail is for the use of only the individual or entity named above.? If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Mon, 25 Feb 2013 13:47:34 -0800 From: "Karla J. C." Subject: [Histonet] Dako autostainer service To: Message-ID: <000f01ce13a1$b8de93a0$2a9bbae0$@dermatopathologynorthwest.com> Content-Type: text/plain; charset="us-ascii" Hi All, Help.Dako just recently informed us that they would no longer provide a Service contract for our immuno autostainer. Is anyone else experiencing this? Does anyone know of any other service people that may be able to work on this equipment (model 3400 year 2003)? We are located in Seattle. Thanks, Karla Carlmas Dermatopathology Northwest ------------------------------ Message: 15 Date: Tue, 26 Feb 2013 01:55:58 -0600 From: Subject: [Histonet] RE: CAP checklist question To: , Message-ID: <4BF03F5404EBDE409AF9232DA74B9DED2DFF10BF41@FWDCWPMSGCMS09.hca.corpad.net> Content-Type: text/plain; charset="us-ascii" We put in a procedure stating that should we change the schedule we will test a variety of tissue (10 different types) and have the pathologist assess the run. We have not changed a schedule in years but we seek to comply... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie Sent: Monday, February 25, 2013 1:21 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP checklist question Hi gang.. I am working on a self-inspection CAP checklist and came across the following: ANP.23120 Tissue Processor Tissue processing schedules are validated. Note: New tissue processing schedules must be validated against the standard laboratory processing schedule. Evidence of compliance: Written procedure for validation of new tissue processing schedules AND WC records documenting validation What do you all make of this?? We have not had any new processing schedules introduced into our lab in years. So, I really don't know how to proceed on this one. Any insite will be greatly appreciated. Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.hannen@parrishmed.com ============= "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ============= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 16 Date: Tue, 26 Feb 2013 09:14:28 -0500 From: "Amy Porter" Subject: [Histonet] Question for Alkaline Phosphatase / Biotin System Users To: Message-ID: <000d01ce142b$96d73ac0$c485b040$@edu> Content-Type: text/plain; charset="us-ascii" We are using standard avidin / biotin staining with alkaline phosphatase labeling and sometimes, not every time and not every slide in the same run we get residual blotchy spots and streaking of the reaction on and off the tissue. We have increased to two rinses after primary and two rinses after alk phos reagent prior to substrate; still happening on some slides...anyone else have this experience or type of problem? Any experiences or suggestions are appreciated. Just can't figure out what is causing it.. Amy S. Porter, HT(ASCP) QIHC Michigan State University Investigative HistoPathology Laboratory portera@msu.edu ------------------------------ Message: 17 Date: Tue, 26 Feb 2013 09:27:03 -0600 From: "Horn, Hazel V" Subject: RE: [Histonet] Waste Alcohol To: "'Rene J Buesa'" , "Scott, Allison D" , "histonet@lists.utsouthwestern.edu" Message-ID: <25A4DE08332B19499904459F00AAACB719BE13563A@EVS1.archildrens.org> Content-Type: text/plain; charset="iso-8859-1" The ability to put alcohol down the drain is regulated by your wastewater utility. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Monday, February 25, 2013 3:53 PM To: Scott, Allison D; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Waste Alcohol I always dumped the ethanol down the drain. I never recycled it because it takes almost 3 times more time that recycling xylene (3 times the cost) and the recycled alcohol is seldom of good quality. When I stopped using xylene I had no more use (and exposure) when recycling. Ren? J. From: "Scott, Allison D" To: "histonet@lists.utsouthwestern.edu" Sent: Monday, February 25, 2013 3:27 PM Subject: [Histonet] Waste Alcohol Hello to all in histoland.? Is there anyone putting alcohol into a waste drum like for xylene.? We have a company that takes away our xylene.? Now the safety people are suggesting that we treat the alcohol the same way.? We have always poured the alcohol down the drain.? It seems very expensive.? Your help in this matter will be greatly appreciated. Allison Scott HT(ASCP) Supervisor, Histology Lab LBJ Hospital Harris Health System Office: 713-566-5287 Lab: 713-566-5287 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged.? This e-mail may also be confidential and/or privileged under Texas law.? The e-mail is for the use of only the individual or entity named above.? If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ------------------------------ Message: 18 Date: Tue, 26 Feb 2013 10:55:14 -0500 From: "Podawiltz, Thomas" Subject: RE: [Histonet] Waste Alcohol To: "Horn, Hazel V" , 'Rene J Buesa' , "Scott, Allison D" , "histonet@lists.utsouthwestern.edu" Message-ID: <38667E7FB77ECD4E91BFAEB8D986386324FCF3C6D9@LRGHEXVS1.practice.lrgh.org> Content-Type: text/plain; charset="iso-8859-1" Reagent Alcohol contains methanol, which is a U-listed EPA hazardous chemical and cannot be poured down the drain. Tom Podawiltz HT (ASCP) Histology Section Head/Laboratory Safety Officer. LRGHealthcare Laconia, NH 03246 603-524-3211 ext: 3220 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Tuesday, February 26, 2013 10:27 AM To: 'Rene J Buesa'; Scott, Allison D; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Waste Alcohol The ability to put alcohol down the drain is regulated by your wastewater utility. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Monday, February 25, 2013 3:53 PM To: Scott, Allison D; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Waste Alcohol I always dumped the ethanol down the drain. I never recycled it because it takes almost 3 times more time that recycling xylene (3 times the cost) and the recycled alcohol is seldom of good quality. When I stopped using xylene I had no more use (and exposure) when recycling. Ren? J. From: "Scott, Allison D" To: "histonet@lists.utsouthwestern.edu" Sent: Monday, February 25, 2013 3:27 PM Subject: [Histonet] Waste Alcohol Hello to all in histoland.? Is there anyone putting alcohol into a waste drum like for xylene.? We have a company that takes away our xylene.? Now the safety people are suggesting that we treat the alcohol the same way.? We have always poured the alcohol down the drain.? It seems very expensive.? Your help in this matter will be greatly appreciated. Allison Scott HT(ASCP) Supervisor, Histology Lab LBJ Hospital Harris Health System Office: 713-566-5287 Lab: 713-566-5287 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged.? This e-mail may also be confidential and/or privileged under Texas law.? The e-mail is for the use of only the individual or entity named above.? If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. ------------------------------ Message: 19 Date: Tue, 26 Feb 2013 09:56:12 -0600 From: "Brendal Finlay" Subject: Re: [Histonet] Waste Alcohol To: "Rene J Buesa" , "Scott, Allison D" , histonet@lists.utsouthwestern.edu Message-ID: <4fcdbb6eed8fb2fc1e5ea8e615ce00ee@medicalcenterclinic.com> Content-Type: text/plain; charset="utf-8" You??will probably want to contact your local or state office of environmental protection to find out what is required in your area.?? ?? The EPA lists hazardous wastes in 40 CFR 261.30.?? Even if your waste is not on this list, it may have hazardous characteristics such as: ignitability, corrosivity, reactivity, and toxicity characteristic.?? Ignitability pertains to flashpoints less than 140 F and/or aqueous solutions with alcohol content greater than or equal to 24 percent.?? ?? Florida's DEP has different regulations dependent upon the quantity of hazardous waste generated.?? Some localities may require approval to dispose of alcohol down the drain.?? If you don't have this approval, you have to dispose of the waste according to??your state??and??federalregulations.?? ?? To reduce the quantity of hazardous waste we generate, we began to recycle using CBG Biotech's distillation unit.?? We have had great results with it.?? While alcohol does take longer to recycle than xylene, our supply costs have been greatly reduced and we have worked this into our weekly schedule very well. ?? Some things to consider when using recycled alcohol: Test each finished alcohol run with a hydrometer to know what percentage it is.?? Do not use recycled alcohol at the end of the stain line after eosin (it will wash the eosin out). Do not use recycled alcohol as the last alcohol before xylene on the processor. Finally, do not recycle the first alcohols on the stain line after xylene (at least on our recycler). This is disposed of in our flammable waste containers to be picked up. ?? Here???s a link to the EPA???s managing hazardous waste pdf - http://www.epa.gov/osw/hazard/generation/sqg/handbook/k01005.pdf Brendal Finlay, HT (ASCP) http://medicalcenterclinic.com -----Original message----- From: Rene J Buesa rjbuesa@yahoo.com Date: Mon, 25 Feb 201316:53:22 -0600 To: "Scott, Allison D" Allison.Scott@harrishealth.org, "histonet@lists.utsouthwestern.edu" histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Waste Alcohol I always dumped the ethanol down the drain. I never recycled it because it takes almost 3 times more time that recycling xylene (3 times the cost) and the recycled alcohol is seldom of good quality. When I stopped using xylene I had no more use (and exposure) when recycling. Ren?? J. From: "Scott, Allison D" To: "histonet@lists.utsouthwestern.edu" Sent: Monday, February 25, 2013 3:27 PM Subject: [Histonet] Waste Alcohol Hello to all in histoland.?? Is there anyone putting alcohol into a waste drum like for xylene.?? We have a company that takes away our xylene.?? Now the safety people are suggesting that we treat the alcohol thesame way.?? We have always poured the alcohol down the drain.?? It seems very expensive.?? Your help in this matter will be greatly appreciated. Allison Scott HT(ASCP) Supervisor, Histology Lab LBJ Hospital Harris Health System Office: 713-566-5287 Lab: 713-566-5287 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged.?? This e-mail may also be confidential and/or privileged under Texas law.?? The e-mail is forthe use of only the individual or entity named above.?? If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 20 Date: Tue, 26 Feb 2013 09:33:37 -0800 (PST) From: Sheila Fonner Subject: [Histonet] streaks To: "cthornton@dahlchase.com" , Histonet Message-ID: <1361900017.78056.YahooMailNeo@web140605.mail.bf1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi Claire, ? I may be grasping at straws here, but I went to an NSH workshop once where this very question was posed.? You won't believe what the cause was.? They had a bad lot of blades.? The whole lot was nicked, so changing to a new knife or a new box of knives did not help the problem.? Changing the lot did the trick.? I don't know if this is your problem or not, but I thought it was very interesting. ? Sheila HT (ASCP) Knoxville, TN ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 111, Issue 37 ***************************************** From rjbuesa <@t> yahoo.com Tue Feb 26 15:58:34 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Feb 26 16:02:44 2013 Subject: [Histonet] Gram stain on decalcified bone In-Reply-To: <06E342B6098ED9478347E1407764C80407C6DC53@VETMXHT.ads.vet.k-state.edu> References: <06E342B6098ED9478347E1407764C80407C6DC53@VETMXHT.ads.vet.k-state.edu> Message-ID: <1361915914.58499.YahooMailNeo@web163104.mail.bf1.yahoo.com> Yes, they have been affected. Ren? J. From: Shelly Christenson To: "histonet@lists.utsouthwestern.edu" Sent: Tuesday, February 26, 2013 4:11 PM Subject: [Histonet] Gram stain on decalcified bone I have a pathologist that wants a gram stain on decalcified bone. We are currently using the Twort's gram stain and the stain did not stain the bacteria in the bone sample, but the control tissue did stain positive. I was wondering if the acid in the decal solution could have affected the bacteria in the bone sample. I'm not for sure how long the bone was decaled, the H&E doesn't look like it was over decaled. Does anyone know of a gram stain that will work on decalcified bone? Thank you for your help, Shelly Christenson HT(ASCP) Veterinary Diagnostic Lab Mosier Hall L-216 Kansas State University Manhattan, Kansas 66506 785/532-4464 christen@vet.k-state.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Susan.Walzer <@t> HCAHealthcare.com Wed Feb 27 02:04:48 2013 From: Susan.Walzer <@t> HCAHealthcare.com (Susan.Walzer@HCAHealthcare.com) Date: Wed Feb 27 02:10:58 2013 Subject: [Histonet] streaks In-Reply-To: <1361900017.78056.YahooMailNeo@web140605.mail.bf1.yahoo.com> References: <1361900017.78056.YahooMailNeo@web140605.mail.bf1.yahoo.com> Message-ID: <4BF03F5404EBDE409AF9232DA74B9DED2DFF1FF1A4@FWDCWPMSGCMS09.hca.corpad.net> Many vendors are pushing different blades...but the only blade that is superior is the Accu-edge. If you are using any other you will very likely get inferior sections. That is 30 years of experience using Accu-edge blades always trying others and always going back to the best. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Fonner Sent: Tuesday, February 26, 2013 12:34 PM To: cthornton@dahlchase.com; Histonet Subject: [Histonet] streaks Hi Claire, ? I may be grasping at straws here, but I went to an NSH workshop once where this very question was posed.? You won't believe what the cause was.? They had a bad lot of blades.? The whole lot was nicked, so changing to a new knife or a new box of knives did not help the problem.? Changing the lot did the trick.? I don't know if this is your problem or not, but I thought it was very interesting. ? Sheila HT (ASCP) Knoxville, TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pitts.jaclyn <@t> gmail.com Wed Feb 27 07:27:42 2013 From: pitts.jaclyn <@t> gmail.com (Jaclyn Pitts) Date: Wed Feb 27 07:27:45 2013 Subject: [Histonet] HELP please! Message-ID: Hey everyone. I Need some help with my processor. It is a thermo scientific stp 120. I came in today and when I went to puch the button to shut the alarm off, I was shocked. The machine shocked me and then the little green display screen went blank. I managed to get my blocks off the machine and I think that I could still run programs but without the display working how am I to know what program it is on!! What can I do? The manual doesnt say anything about what to do about this. -- *Jaclyn Pitts, HT(ASCP)CM* 218-454-3520 Dermatology Professionals, PA 15167 Edgewood Dr. Suite 200 Baxter, MN 56425 From PAMarcum <@t> uams.edu Wed Feb 27 07:40:27 2013 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Wed Feb 27 07:40:32 2013 Subject: [Histonet] streaks In-Reply-To: <4BF03F5404EBDE409AF9232DA74B9DED2DFF1FF1A4@FWDCWPMSGCMS09.hca.corpad.net> References: <1361900017.78056.YahooMailNeo@web140605.mail.bf1.yahoo.com> <4BF03F5404EBDE409AF9232DA74B9DED2DFF1FF1A4@FWDCWPMSGCMS09.hca.corpad.net> Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA32973992AA@Mail2Node2.ad.uams.edu> They were using AccuEdge here for years and we changed to the Thermo MX35 Premier 3 years ago with no issues. In fact everyone was very upset for about a month and then they did not want to change back. So, it really depends on the knife and as I was told after using them for month they could not tell a difference. If you went back with a pack of AccuEdge, they were just as happy to stay with the Premier blade. I started with the steel knives almost 47 years ago and have seen so many changes and improvements in knives that I have learned to try something new and see rather than buy by brand or habit only. Sometimes it works and sometimes it does not however; you never know till you give it a good try. Pam Marcum UAMS Histology Supervisor -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Susan.Walzer@HCAHealthcare.com Sent: Wednesday, February 27, 2013 2:05 AM To: fourfonners@yahoo.com; cthornton@dahlchase.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] streaks Many vendors are pushing different blades...but the only blade that is superior is the Accu-edge. If you are using any other you will very likely get inferior sections. That is 30 years of experience using Accu-edge blades always trying others and always going back to the best. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Fonner Sent: Tuesday, February 26, 2013 12:34 PM To: cthornton@dahlchase.com; Histonet Subject: [Histonet] streaks Hi Claire, ? I may be grasping at straws here, but I went to an NSH workshop once where this very question was posed.? You won't believe what the cause was.? They had a bad lot of blades.? The whole lot was nicked, so changing to a new knife or a new box of knives did not help the problem.? Changing the lot did the trick.? I don't know if this is your problem or not, but I thought it was very interesting. ? Sheila HT (ASCP) Knoxville, TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From CThornton <@t> dahlchase.com Wed Feb 27 07:42:17 2013 From: CThornton <@t> dahlchase.com (Clare Thornton) Date: Wed Feb 27 07:42:24 2013 Subject: [Histonet] streaks In-Reply-To: <41D3A1AF6FEF0643BDC89E0516A6EA32973992AA@Mail2Node2.ad.uams.edu> References: <1361900017.78056.YahooMailNeo@web140605.mail.bf1.yahoo.com> <4BF03F5404EBDE409AF9232DA74B9DED2DFF1FF1A4@FWDCWPMSGCMS09.hca.corpad.net> <41D3A1AF6FEF0643BDC89E0516A6EA32973992AA@Mail2Node2.ad.uams.edu> Message-ID: Thanks everyone for the suggestions..it's appearing that it was a bad lot of blades. We switched to a new lot and there has been a noticeable improvement. We are giving it a day or two to be sure it wasn't a combination of problems but hopefully we will get beautiful sections once again! Clare Clare J. Thornton, HTL(ASCP)QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com -----Original Message----- From: Marcum, Pamela A [mailto:PAMarcum@uams.edu] Sent: Wednesday, February 27, 2013 8:40 AM To: 'Susan.Walzer@HCAHealthcare.com'; fourfonners@yahoo.com; Clare Thornton; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] streaks They were using AccuEdge here for years and we changed to the Thermo MX35 Premier 3 years ago with no issues. In fact everyone was very upset for about a month and then they did not want to change back. So, it really depends on the knife and as I was told after using them for month they could not tell a difference. If you went back with a pack of AccuEdge, they were just as happy to stay with the Premier blade. I started with the steel knives almost 47 years ago and have seen so many changes and improvements in knives that I have learned to try something new and see rather than buy by brand or habit only. Sometimes it works and sometimes it does not however; you never know till you give it a good try. Pam Marcum UAMS Histology Supervisor -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Susan.Walzer@HCAHealthcare.com Sent: Wednesday, February 27, 2013 2:05 AM To: fourfonners@yahoo.com; cthornton@dahlchase.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] streaks Many vendors are pushing different blades...but the only blade that is superior is the Accu-edge. If you are using any other you will very likely get inferior sections. That is 30 years of experience using Accu-edge blades always trying others and always going back to the best. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Fonner Sent: Tuesday, February 26, 2013 12:34 PM To: cthornton@dahlchase.com; Histonet Subject: [Histonet] streaks Hi Claire, ? I may be grasping at straws here, but I went to an NSH workshop once where this very question was posed.? You won't believe what the cause was.? They had a bad lot of blades.? The whole lot was nicked, so changing to a new knife or a new box of knives did not help the problem.? Changing the lot did the trick.? I don't know if this is your problem or not, but I thought it was very interesting. ? Sheila HT (ASCP) Knoxville, TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From mhale <@t> MiracaLS.com Wed Feb 27 07:58:28 2013 From: mhale <@t> MiracaLS.com (Hale, Meredith) Date: Wed Feb 27 07:58:34 2013 Subject: [Histonet] KY HT Positions Message-ID: <0E828EC51C7CC445A51E53F81B64E8C71ADC98@s-irv-exchmb.PathologyPartners.intranet> Please No Recruiter Calls : Great opportunity for Histotechnician's in Crestview Hills, KY ! Tri-State Gastroenterology Associates is a multi-physician practice located in Northern Kentucky. Its mission is "To provide compassionate, high quality, cost-effective care to patients' with gastrointestinal problems" Looking for 2 Full Time HT/HTL's ( 32 hours a week is full time employment at this practice ) * Meet CLIA Grossing Requirements : CFR 493.1489, http://wwwn.cdc.gov/clia/regs/toc.aspx/ ,prior experience grossing GI specimens * Supervisor experience preferred * HT/HTL ASCP Certified * Experience with CLIA and CAP * Experience writing and maintaining policies and procedures * Prior laboratory start up experience is preferred * Ability to work independently Duties include: * Grossing * Embedding * Microtomy * Staining; routine and special stains only * Maintain supply orders and laboratory budget * Ability to be flexible and take on additional duties' as needed * Ability to work independently * Maintenance of laboratory for inspections * Maintenance of quality records 2 positions' that offers competitive rates and flexible hours. Interested applicants should contact Meredith Hale; phone 214-596-2219 or through email mhale@miracals.com Meredith Hale HT (ASCP)cm Director External Sales Support Miraca Life Sciences 6655 North MacArthur Blvd. Irving , Texas 75039 Office: 214-596-2219 Cell: 469-648-8253 Fax: 1-866-688-3280 mhale@miracals.com> From histojoe <@t> hotmail.com Wed Feb 27 08:10:51 2013 From: histojoe <@t> hotmail.com (Histo Joe) Date: Wed Feb 27 08:10:56 2013 Subject: [Histonet] Arm cutting length Message-ID: Hi, I'm trying to find out if anyone can tell me what can cause a sudden distance change in the length of rotation on an HM 450 sliding freezing microtome. range of motion was almost full length of microtome then suddenly range is reduced to barely clearing base which causes a potential injury to cutter. Thanks,Joe From rjbuesa <@t> yahoo.com Wed Feb 27 09:50:09 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Feb 27 09:50:13 2013 Subject: [Histonet] Arm cutting length In-Reply-To: References: Message-ID: <1361980209.16714.YahooMailNeo@web163102.mail.bf1.yahoo.com> You should ask the manufacturer Ren? J. From: Histo Joe To: histonet@lists.utsouthwestern.edu Sent: Wednesday, February 27, 2013 9:10 AM Subject: [Histonet] Arm cutting length Hi, I'm trying to find out if anyone can tell me what can cause a sudden distance change in the length of rotation on an HM 450 sliding freezing microtome. range of motion was almost full length of microtome then suddenly range is reduced to barely clearing base which causes a potential injury to cutter. Thanks,Joe ??? ??? ??? ? ??? ??? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 <@t> earthlink.net Wed Feb 27 09:51:02 2013 From: relia1 <@t> earthlink.net (Pam Barker) Date: Wed Feb 27 09:51:07 2013 Subject: [Histonet] RELIA Histology Careers Bulletin 2-27-2013 Exciting Opportunities and a Special Gift in honor of histotechnology professionals day. Message-ID: <150001ce1502$3f01e610$bd05b230$@earthlink.net> Hi Histonetters! I hope you are having a great day. I wanted to drop you a line and tell you about some of the opportunities that I am working on and offer you a gift. The gift is I have a copy of the Histology Professionals Day logo that you can add to your signature or your Facebook page or whatever you would like to do with it. If you don't already have it and would like me to send it to you please let me know. I also have some job opportunities to share with you. All of my opportunities are with some of the best facilities in the nation. My clients offer excellent compensation and benefits and in some cases relocation assistance. Here is a list of my current openings: Histology Management Histology Manager - Charlotte, NC Strong IHC required for this growing lab! Histology Manager - Boston, MA A great team of histotechs is waiting for you! Lead Histotechnologist - Staunton, VA - Train and Mentor New Techs! Pathology Manager - San Francisco - Prestigious institution academic exper. required. Lead IHC Tech - NYC, NY Nationally Recognized Facility in NYC! Histology Manager - Los Angeles - Challenging position with a busy reference lab. Histology Supervisor - Lancaster, PA Private lab with a great team to work with. HT/HTL Positions: Louisville, KY - Great people to work with in a beautiful environment Charlotte, NC- Brand new lab! Brand new team! Brand new day!! Collegeville, PA - Excellent opportunity to grow and work with a top notch team! Lancaster, PA - Private Lab with opportunity to learn grossing and IHC. Portland, ME - Fast-paced lab with excellent compensation and benefits. Long Island, NY - Advanced techniques and excellent $$ Harrisonburg, VA - Train and Mentor new techs NYC, NY - NY License required, Leading edge Facility Other Opportunities: Molecular Diagnostic Tech - Philadelphia Pathologists' Assistant - Austin, TX Molecular Diagnostics Manager - Springfield, OR/Vancouver, WA Electron Microscopy Tech - Long Island, NY I realize that there are a lot of recruiters out there right now trying to work with histo techs and I appreciate your support and respect your needs. Remember I offer over 25 years of experience as a recruiter and for over 10 years I have dedicated my practice solely to placing histology professionals like you. I have always and continue to advocate for you and your career. Be careful as you pursue positions with inexperienced recruiters, if you would like a copy of the blog post I wrote last year titled "Essential Tips for working with a Recruiter" I would be happy to send you a copy. If you or anyone you know might be interested in any of these opportunities please contact me. Remember you can earn a 500.00 referral fee for a referral whether it is a person looking for a job or a job lead. Thanks-Pam Right Place, Right Time, Right Move with RELIA! Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From jmbrooksokstate <@t> gmail.com Wed Feb 27 10:12:44 2013 From: jmbrooksokstate <@t> gmail.com (Joseph Brooks) Date: Wed Feb 27 10:12:51 2013 Subject: [Histonet] Unencased Ameoba Stain Message-ID: Hello All, One of our Neuropath docs is inquiring about a special stain for unencased ameobas in cornea biopsies. I did a search and Gridley's Method was the best option that appreaded. Is there someone that could either verify this stain will work on this organism or let me know what you stain you are using? Thanks in advance. Matt Brooks From barrickstacey <@t> yahoo.com Wed Feb 27 10:20:38 2013 From: barrickstacey <@t> yahoo.com (Stacey Barrick) Date: Wed Feb 27 10:20:41 2013 Subject: [Histonet] Mayer's Hematoxylin on frozen tissue Message-ID: <1361982038.2419.YahooMailNeo@web141403.mail.bf1.yahoo.com> Hi everyone,? We are trying to stain frozen cut sections of aorta with Mayer's Hematoxylin following Oil Red O staining. We cannot get hematoxylin staining to work. We are mainly seeing blue background but not labeling of nuclei. Tissue is fixed with 4% PFA prior to sectioning. After tissue is cut; sections ?are stained with Oil Red O ????????? ddH2O 2? ????????? 60% isopropanol 30s ????????? Oil Red O 18? ????????? 60% isopropanol 30s ????????? 2x ddH2O 1? Sections are then stained with Sigma Mayer's Hematoxylin? ?? ? Rinse in deionized water ?? ?Stain in Mayer's Hematoxylin 1-5 min ? ?Rinse in running tap water until nuclei are blue ?? Rinse in deionized water We have tried staining in Hematoxylin for 3 min up to 15 mins We have also tried rinsing 1 min up to 15 mins (checking at for staining at various timepoints) and always see the same result -some blue staining but no clear nuclear staining. Does anyone have any suggestions as to why this isn't working? Thanks!! Stacey From ssegal2 <@t> slu.edu Wed Feb 27 10:30:50 2013 From: ssegal2 <@t> slu.edu (Salomao Segal) Date: Wed Feb 27 10:30:58 2013 Subject: [Histonet] polarizing microscopy Message-ID: I own an old Zeiss microscope (Universal) and would like to have the capability of performing polarized light microscopy studies. I am particularly interested in exploiting the birefringent properties of myelinated fibers in peripheral nerves. My questions are: 1) what are the microscope parts needed for polarizing microscopy? (condenser, filters, stage, objectives, etc.) 2) any good source about these techniques for the beginner? Any guidance would be greatly appreciated. Thanks -- Solomon Segal, M.D. Assistant Professor of Anatomy in Surgery Center for Anatomical Science and Education (CASE) Department of Surgery School of Medicine Saint Louis University 1402 South Grand Blvd. Schwitalla Hall - 3rd Floor - M310 Saint Louis, MO, 63104 office: 314 977 8023 laboratory: 314 977 8080 CASE: 314 977 8027 FAX: 314 977 5127 e-mail: ssegal2@slu.edu http://medschool.slu.edu/anatomy/ http://slu.academia.edu/SolomonSegal https://sites.google.com/a/slu.edu/segal-laboratory/ https://sites.google.com/a/slu.edu/dr-segal-s-clinical-anatomy-website/ From rjbuesa <@t> yahoo.com Wed Feb 27 10:31:54 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Feb 27 10:31:58 2013 Subject: [Histonet] Unencased Ameoba Stain In-Reply-To: References: Message-ID: <1361982714.82878.YahooMailNeo@web163102.mail.bf1.yahoo.com> The "standard" amoeba stain is Heidenhain's iron hematoxylin. Ren? J. From: Joseph Brooks To: histonet@lists.utsouthwestern.edu Sent: Wednesday, February 27, 2013 11:12 AM Subject: [Histonet] Unencased Ameoba Stain Hello All, One of our Neuropath docs is inquiring about a special stain for unencased ameobas in cornea biopsies.? I did a search and Gridley's Method was the best option that appreaded.? Is there someone that could either verify this stain will work on this organism or let me know what you stain you are using?? Thanks in advance. Matt Brooks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Feb 27 10:33:48 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Feb 27 10:33:54 2013 Subject: [Histonet] Mayer's Hematoxylin on frozen tissue In-Reply-To: <1361982038.2419.YahooMailNeo@web141403.mail.bf1.yahoo.com> References: <1361982038.2419.YahooMailNeo@web141403.mail.bf1.yahoo.com> Message-ID: <1361982828.84854.YahooMailNeo@web163101.mail.bf1.yahoo.com> Mayer's hemaotoxylin (a "progressive" hematoxylin) is not adequate for FS. Try Harris hematoxylin BUT stain first with the hematoxylin and with ORO after wards to obtain better results. Ren? J. From: Stacey Barrick To: "Histonet@lists.utsouthwestern.edu" Sent: Wednesday, February 27, 2013 11:20 AM Subject: [Histonet] Mayer's Hematoxylin on frozen tissue Hi everyone,? We are trying to stain frozen cut sections of aorta with Mayer's Hematoxylin following Oil Red O staining. We cannot get hematoxylin staining to work. We are mainly seeing blue background but not labeling of nuclei. Tissue is fixed with 4% PFA prior to sectioning. After tissue is cut; sections ?are stained with Oil Red O ????????? ddH2O 2? ????????? 60% isopropanol 30s ????????? Oil Red O 18? ????????? 60% isopropanol 30s ????????? 2x ddH2O 1? Sections are then stained with Sigma Mayer's Hematoxylin? ?? ? Rinse in deionized water ?? ?Stain in Mayer's Hematoxylin 1-5 min ? ?Rinse in running tap water until nuclei are blue ?? Rinse in deionized water We have tried staining in Hematoxylin for 3 min up to 15 mins We have also tried rinsing 1 min up to 15 mins (checking at for staining at various timepoints) and always see the same result -some blue staining but no clear nuclear staining. Does anyone have any suggestions as to why this isn't working? Thanks!! Stacey _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Feb 27 10:39:51 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Feb 27 10:41:01 2013 Subject: [Histonet] polarizing microscopy In-Reply-To: References: Message-ID: <1361983191.87131.YahooMailNeo@web163101.mail.bf1.yahoo.com> The best and less expensive?solution is to go to a photography store and buy a plastic polarizing filter. Cut a round piece and place it in the condenser filter ring and cut another (of much smaller diameter)?plastic?filter?and place it in the filter holder of your microscope ocular. The light getting into the specimen coming from the condenser will be partially polarized and moving the ocular in a circular manner, you will obtain the polarization of the field of view and the specimen. Ren? J. From: Salomao Segal To: histonet@lists.utsouthwestern.edu Sent: Wednesday, February 27, 2013 11:30 AM Subject: [Histonet] polarizing microscopy I own an old Zeiss microscope (Universal) and would like to have the capability of performing polarized light microscopy studies. I am particularly interested in exploiting the birefringent properties of myelinated fibers in peripheral nerves. My questions are: 1) what are the microscope parts needed for polarizing microscopy? (condenser, filters, stage, objectives, etc.) 2) any good source about these techniques for the beginner? Any guidance would be greatly appreciated. Thanks -- Solomon Segal, M.D. Assistant Professor of Anatomy in Surgery Center for Anatomical Science and Education (CASE) Department of Surgery School of Medicine Saint Louis University 1402 South Grand Blvd. Schwitalla Hall - 3rd Floor - M310 Saint Louis, MO, 63104 office: 314 977 8023 laboratory: 314 977 8080 CASE: 314 977 8027 FAX: 314 977 5127 e-mail: ssegal2@slu.edu http://medschool.slu.edu/anatomy/ http://slu.academia.edu/SolomonSegal https://sites.google.com/a/slu.edu/segal-laboratory/ https://sites.google.com/a/slu.edu/dr-segal-s-clinical-anatomy-website/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> gmail.com Wed Feb 27 12:19:22 2013 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Wed Feb 27 12:19:27 2013 Subject: [Histonet] Re: Unencased Amoeba Stain Message-ID: Matt Brooks asks: >>One of our Neuropath docs is inquiring about a special stain for unencased [amoebae] in cornea biopsies. I did a search and Gridley's method was the best option that appreaded [sic]. Is there someone that could either verify this stain will work on this organism or let me know what you stain you are using?<< and Ren? Buesa suggests Heidenhain's iron hematoxylin. Corneal ulcers are caused by Acanthamoeba and related species, rather than by Entamoeba histolytica. Google >>amoeba corneal stain<< - some references suggest a fluorescent stain such as calcofluor white, if you have access to a fluorescence microscope. Iron hematoxylins provide exquisite nuclear detail, but are challenging to set up for the first time (I've done it), and are not specific. Periodic acid Schiff (PAS) is often used to demonstrate the glycogen in Entamoeba histolytica trophozoites, but I don't know if the other pathogenic amoebae contain glycogen. My advice would be to ask one of the academic eye pathologists. My choice would be Dr. Hans Grossniklaus at Emory, but there are a good many more. And remember you'll need to find a control slide. Bob Richmond Samurai Pathologist Maryville TN From joelleweaver <@t> hotmail.com Wed Feb 27 12:59:00 2013 From: joelleweaver <@t> hotmail.com (=?utf-8?B?am9lbGxld2VhdmVyQGhvdG1haWwuY29t?=) Date: Wed Feb 27 12:59:05 2013 Subject: [Histonet] Thoughts/ opinions on Ultra Inform HER2 dual FISH Message-ID: Hello everyone I have been asked to collect the opinions of any users of the "INFORM" HER2 dual ISH for identification of breast cancer patients for Trastuzumab. I would appreciate any feedback from users on technical issues, clinical value, or other things you might wish to share. I appreciate the assistance. Joelle Sent from my Verizon Wireless 4G LTE Smartphone From gayle.callis <@t> bresnan.net Wed Feb 27 13:15:09 2013 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Wed Feb 27 13:16:54 2013 Subject: [Histonet] Re: Oil Red O and Mayers Message-ID: <000401ce151e$c3f3cca0$4bdb65e0$@bresnan.net> Stacey, You wrote: You wrote: We are trying to stain frozen cut sections of aorta with Mayer's Hematoxylin following Oil Red O staining. We cannot get hematoxylin staining to work. We are mainly seeing blue background but not labeling of nuclei. Tissue is fixed with 4% PFA prior to sectioning. After tissue is cut; sections are stained with Oil Red O . ddH2O 2' . 60% isopropanol 30s . Oil Red O 18' . 60% isopropanol 30s . 2x ddH2O 1' Sections are then stained with Sigma Mayer's Hematoxylin . Rinse in deionized water . Stain in Mayer's Hematoxylin 1-5 min . Rinse in running tap water until nuclei are blue . Rinse in deionized water We have tried staining in Hematoxylin for 3 min up to 15 mins We have also tried rinsing 1 min up to 15 mins (checking at for staining at various timepoints) and always see the same result -some blue staining but no clear nuclear staining. **************************************************************************** *********************************************************************** Possibilities here are: 1. Not getting rid of the isopropyl alcohol adequately. Try a longer rinse than 1 minute to rehydrate the section, and add an additional DI water rinse to make sure the alcohol is removed However there is a much better Oil Red O stain than the one you could be using now (you didn't give method e.g. messy Oil Red O with propylene glycol, and without isopropyl rinsing. I am forwarding Churukians Oil Red O under separate cover which has never failed on our frozen sections. It is easy to make up and use. We also prefer Gill 2 hematoxylin staining which takes less time and requires a mild bluing solution or you can simply use warm water to blue the section. If Gill 2 seems too dark, use Gill. I have the original Journal of Histotechnology publication by Churukian if you want it. We always do Oil Red O stain before counterstaining with hematoxylin. 2. How fresh is your Mayers? It could be outdated or needs changing? Also, after final tap water rinse, we coverslip without an extra deionized water rinse. Good luck Gayle Callis HTL/HT/MT(ASCP) From kathleenbess <@t> sbcglobal.net Wed Feb 27 13:21:29 2013 From: kathleenbess <@t> sbcglobal.net (Kathleen Bess) Date: Wed Feb 27 13:22:38 2013 Subject: [Histonet] Chemicals with no expiration Message-ID: <1361992889.33418.YahooMailClassic@web181301.mail.ne1.yahoo.com> Hi all, ? First time using histonet- I read earlier that reagents/chemicals used in the lab with no expiration date are to be labeled with an expiration date of 5 years after having been opened. Is there literature on that? I need to update my policy and prodedure for CAP. Also, this maysaound silly, but do you label your DI water too? ? Thanks in advance, ? Kathy Fernandez Lead Histotech Saddleback Memorial Medical Center Laguna Hills, CA From Timothy.Morken <@t> ucsfmedctr.org Wed Feb 27 13:36:51 2013 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Wed Feb 27 13:40:39 2013 Subject: [Histonet] Chemicals with no expiration In-Reply-To: <1361992889.33418.YahooMailClassic@web181301.mail.ne1.yahoo.com> References: <1361992889.33418.YahooMailClassic@web181301.mail.ne1.yahoo.com> Message-ID: <761E2B5697F795489C8710BCC72141FF061420@ex07.net.ucsf.edu> Kathy, I actually posed that question to Fisher Scientific and got a letter back saying that if the chemical is known to be unstable then they will give it an expiration date (shown below). Chemicals that are very stable are considered to have indefinite shelf life. Here we label liquids at 3 years and solids at 5 years. Note that anhydrous powdered chemicals are less stable than their hydrated versions so could have a shorter shelf life depending on humidity in your lab. In general I try to order chemicals in quantities that will be used up within a year so we maintain a reasonable turnover. That is not possible with some things for which a little goes a long way so we just re-validate those when needed. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center ************************************************************************** The letter from Fisher: Chemical Services Group Technical Helpline (800) 227-6701, opt, #1 Cert. of Analysis and MSDS (201) 703-3165 Fax (201) 703-3159 August 16, 2010 Re: Acetic Acid (A35), Ammonium Hydroxide (A665), Hydrochloric Acid Cat (A144) Dear Tim Morken: This is in reply to your request for information pertaining to shelf life. Some of the chemicals from Fisher may not have an expiration date, simply because those materials should not decompose under normal storage conditions. They should have an indefinite shelf life if they are not contaminated or adulterated. If you are buying a product from Fisher Scientific Company and the product has a known instability, an expiration date will be noted on the label. The stability/shelf life was established by testing sealed and previously opened bottles over the duration of the shelf life period. Unless otherwise specified, the expiration date will be the last day of the month indicated on the label. It is good Chemical Practice, however, not to keep chemicals beyond 3 to 5 years. Over extended periods of time and/or conditions beyond your direct control, degradation of even stable compounds could occur. Generally, the first two digits of the six digit lot number of Fisher Chemicals will indicate the year of manufacture. Storage conditions for all Fisher chemicals should be room temperature unless other wise stated on the label. I trust that you will find this information useful. Please call if we can be of further help. Sincerely, The Fisher Chemical Services Team ************************************************************ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kathleen Bess Sent: Wednesday, February 27, 2013 11:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Chemicals with no expiration Hi all, ? First time using histonet- I read earlier that reagents/chemicals used in the lab with no expiration date are to be labeled with an expiration date of 5 years after having been opened. Is there literature on that? I need to update my policy and prodedure for CAP. Also, this maysaound silly, but do you label your DI water too? ? Thanks in advance, ? Kathy Fernandez Lead Histotech Saddleback Memorial Medical Center Laguna Hills, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Wed Feb 27 13:39:29 2013 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Wed Feb 27 13:40:43 2013 Subject: [Histonet] Chemicals with no expiration In-Reply-To: <1361992889.33418.YahooMailClassic@web181301.mail.ne1.yahoo.com> References: <1361992889.33418.YahooMailClassic@web181301.mail.ne1.yahoo.com> Message-ID: <761E2B5697F795489C8710BCC72141FF061436@ex07.net.ucsf.edu> Oh, BTW, we do label our distilled water with prep and expiration date when it is put in bottles for use on the bench. It is not sterile and will grow stuff if left too long. Usually we use it up quickly but it is good practice to know when any reagent is made and expires. We give it a 4 week expiration. Tim -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kathleen Bess Sent: Wednesday, February 27, 2013 11:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Chemicals with no expiration Hi all, ? First time using histonet- I read earlier that reagents/chemicals used in the lab with no expiration date are to be labeled with an expiration date of 5 years after having been opened. Is there literature on that? I need to update my policy and prodedure for CAP. Also, this maysaound silly, but do you label your DI water too? ? Thanks in advance, ? Kathy Fernandez Lead Histotech Saddleback Memorial Medical Center Laguna Hills, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histotalk <@t> yahoo.com Wed Feb 27 13:42:08 2013 From: histotalk <@t> yahoo.com (David Kemler) Date: Wed Feb 27 13:42:14 2013 Subject: [Histonet] New Program Message-ID: <1361994128.14198.YahooMailNeo@web121501.mail.ne1.yahoo.com> Hello Everyone- ? In case you missed it, our very own Jack Ratliff and Dr Heiko Richter's (not our very own) Part 2 of their HistoTALK http://www.histotalk.com/ interview was produced last week. Very interesting topic - Laser Histology! You're invited to give it a listen. ? Yours, David From dmccaig <@t> ckha.on.ca Wed Feb 27 13:55:20 2013 From: dmccaig <@t> ckha.on.ca (Diana McCaig) Date: Wed Feb 27 13:55:35 2013 Subject: [Histonet] Alcian Blue Message-ID: What is the shelf life of prepared 1% Alcian blue in 3% Acetic Acid? Diana From rjbuesa <@t> yahoo.com Wed Feb 27 13:57:08 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Feb 27 13:57:23 2013 Subject: [Histonet] Alcian Blue In-Reply-To: References: Message-ID: <1361995028.25712.YahooMailNeo@web163101.mail.bf1.yahoo.com> Until the staining starts to weaken Ren? J. From: Diana McCaig To: "'histonet@lists.utsouthwestern.edu'" Sent: Wednesday, February 27, 2013 2:55 PM Subject: [Histonet] Alcian Blue What is the shelf life of? prepared? 1% Alcian blue in 3% Acetic Acid? Diana _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Wed Feb 27 14:03:06 2013 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Wed Feb 27 14:03:18 2013 Subject: [Histonet] Alcian Blue In-Reply-To: <1361995028.25712.YahooMailNeo@web163101.mail.bf1.yahoo.com> References: <1361995028.25712.YahooMailNeo@web163101.mail.bf1.yahoo.com> Message-ID: <14E2C6176416974295479C64A11CB9AE016411AC2746@SBS2K8.premierlab.local> Why would you use a reagent until it starts to weaken, meaning the staining intensity and results are changing over time. In my opinion that's really poor quality control. Stain intensity, specificity and sensitivity should remain constant. Liz -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, February 27, 2013 11:57 AM To: Diana McCaig; 'histonet@lists.utsouthwestern.edu' Subject: Re: [Histonet] Alcian Blue Until the staining starts to weaken Ren? J. From: Diana McCaig To: "'histonet@lists.utsouthwestern.edu'" Sent: Wednesday, February 27, 2013 2:55 PM Subject: [Histonet] Alcian Blue What is the shelf life of prepared 1% Alcian blue in 3% Acetic Acid? Diana _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tkngflght <@t> yahoo.com Wed Feb 27 14:06:57 2013 From: tkngflght <@t> yahoo.com (Cheryl) Date: Wed Feb 27 14:07:03 2013 Subject: [Histonet] Decal on the bench - billing potential Message-ID: <1361995617.22881.YahooMailClassic@web161902.mail.bf1.yahoo.com> A quick question:? Does anyone bill an 88311 decal for processes done on the bench? ? Many thanks--!! Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab by helping one GREAT?Tech at a time.? 281.852.9457?Office 800.756.3309?Phone & Fax? admin@fullstaff.org Sign up for the FREE?newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please?include your name and specialty in the body of the email. From asmith <@t> mail.barry.edu Wed Feb 27 14:21:03 2013 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Wed Feb 27 14:21:12 2013 Subject: [Histonet] Alcian Blue In-Reply-To: <14E2C6176416974295479C64A11CB9AE016411AC2746@SBS2K8.premierlab.local> References: <1361995028.25712.YahooMailNeo@web163101.mail.bf1.yahoo.com> <14E2C6176416974295479C64A11CB9AE016411AC2746@SBS2K8.premierlab.local> Message-ID: Alcian blue lasts until loss of dye onto the sections weakens staining. If one runs many slides through it, it may not last 6 weeks. With light use, it may last 6 years. Allen A. Smith Professor of Anatomy Barry University School of Podiatric Medicine -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala Sent: Wednesday, February 27, 2013 3:03 PM To: Rene J Buesa; Diana McCaig; 'histonet@lists.utsouthwestern.edu' Subject: RE: [Histonet] Alcian Blue Why would you use a reagent until it starts to weaken, meaning the staining intensity and results are changing over time. In my opinion that's really poor quality control. Stain intensity, specificity and sensitivity should remain constant. Liz -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, February 27, 2013 11:57 AM To: Diana McCaig; 'histonet@lists.utsouthwestern.edu' Subject: Re: [Histonet] Alcian Blue Until the staining starts to weaken Ren? J. From: Diana McCaig To: "'histonet@lists.utsouthwestern.edu'" Sent: Wednesday, February 27, 2013 2:55 PM Subject: [Histonet] Alcian Blue What is the shelf life of prepared 1% Alcian blue in 3% Acetic Acid? Diana _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Pat.Patterson <@t> propath.com Wed Feb 27 15:25:48 2013 From: Pat.Patterson <@t> propath.com (Pat Patterson) Date: Wed Feb 27 15:29:04 2013 Subject: [Histonet] IHC Position Dallas Message-ID: <82C7248978CB50469FD6BA68EBBEFE67098AB4EE@exchange.propathlab.com> IMMUNOHISTOCHEMISTRY TECHNICIAN ProPath, a progressive, CAP accredited, high-volume pathology practice in Dallas, Texas is seeking an Immunohistochemistry Technician for its' Immunohistochemistry Lab. Responsibilities include slide preparation (paraffin and frozen sections), IHC staining using our unique manual system, antibody titer preparation, equipment maintenance, supply/reagent inventory maintenance, and QC/QA recording. The ideal candidate will have a minimum of 4 years Histology experience with paraffin microtomy with a variety of different tissue types, prefer at least 1 year immunohistochemistry, immunofluorescence or in situ hybridization and frozen section experience. Working knowledge of IHC theory required, hands on IHC performance desired. If using an automated system we'll easily train you on our manual system. HT (ASCP) or QIHC desired. The hours for the position are 5 p.m. to 1:30 a.m. Monday through Friday. ProPath utilizes leading technology and is a quality oriented pathology laboratory. Benefits include medical, dental, Short and Long Term Disability insurance, a matched 401K plan and more! Don't Follow the Leader! Join the Leader! To apply, please visit www.propath.com EOE Accessibility Accommodations If you require an accommodation to navigate or apply to our careers site, please send your request to accessibility@propath.com. Pat Patterson, HTL(ASCP) Supervisor, Immunohistochemistry ProPath - The Leader in Pathology Services 1355 River Bend Drive Dallas, TX 75247 214-237-1700 x 2027 214-237-1730 fax To learn more about ProPath, please visit http://www.ProPath.com This electronic message is intended to be for the use only of the named recipient and may contain information that is confidential or privileged. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this message in error or are not the named recipient, please notify us immediately by contacting the sender at the electronic mail address noted above, and delete and destroy all copies of this message. From Tony_Reilly <@t> health.qld.gov.au Wed Feb 27 18:30:53 2013 From: Tony_Reilly <@t> health.qld.gov.au (Tony Reilly) Date: Wed Feb 27 18:31:32 2013 Subject: [Histonet] Mayer's Hematoxylin on frozen tissue In-Reply-To: <1361982828.84854.YahooMailNeo@web163101.mail.bf1.yahoo.com> References: <1361982038.2419.YahooMailNeo@web141403.mail.bf1.yahoo.com> <1361982828.84854.YahooMailNeo@web163101.mail.bf1.yahoo.com> Message-ID: <512F31DC.411C.0039.0@health.qld.gov.au> Hi Stacey I have used Mayer's Haematoxylin with an Oil Red O for more than 30 years and it works fine, You need to stain with the Hx before the Oil Red O. 3 Minutes should be enough. regards Tony Tony Reilly B.App.Sc. , M.Sc. Chief Scientist, Anatomical Pathology Pathology Queensland-PA Laboratory ________________________________________________ Health Services Support Agency | Department of Health Level 1, Building 15,Princess Alexandra Hospital Ipswich Road,WOOLLOONGABBA Qld4102 Ph: 07 3176 2412 Mob: 0402 139411 Fax: 07 3176 2930 Email: tony_reilly@health.qld.gov.au Web: www.health.qld.gov.au/qhcss/ >>> Rene J Buesa 2/28/2013 2:33 am >>> Mayer's hemaotoxylin (a "progressive" hematoxylin) is not adequate for FS. Try Harris hematoxylin BUT stain first with the hematoxylin and with ORO after wards to obtain better results. Ren? J. From: Stacey Barrick To: "Histonet@lists.utsouthwestern.edu" Sent: Wednesday, February 27, 2013 11:20 AM Subject: [Histonet] Mayer's Hematoxylin on frozen tissue Hi everyone, We are trying to stain frozen cut sections of aorta with Mayer's Hematoxylin following Oil Red O staining. We cannot get hematoxylin staining to work. We are mainly seeing blue background but not labeling of nuclei. Tissue is fixed with 4% PFA prior to sectioning. After tissue is cut; sections are stained with Oil Red O ? ddH2O 2? ? 60% isopropanol 30s ? Oil Red O 18? ? 60% isopropanol 30s ? 2x ddH2O 1? Sections are then stained with Sigma Mayer's Hematoxylin ? Rinse in deionized water ? Stain in Mayer's Hematoxylin 1-5 min ? Rinse in running tap water until nuclei are blue ? Rinse in deionized water We have tried staining in Hematoxylin for 3 min up to 15 mins We have also tried rinsing 1 min up to 15 mins (checking at for staining at various timepoints) and always see the same result -some blue staining but no clear nuclear staining. Does anyone have any suggestions as to why this isn't working? Thanks!! Stacey _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ********************************************************************************** From Tony_Reilly <@t> health.qld.gov.au Wed Feb 27 19:58:48 2013 From: Tony_Reilly <@t> health.qld.gov.au (Tony Reilly) Date: Wed Feb 27 19:59:43 2013 Subject: [Histonet] Re: Unencased Amoeba Stain In-Reply-To: References: Message-ID: <512F4677.411C.0039.0@health.qld.gov.au> Hi Matt The Heidenhain's stain is good but if you want one that is prettier try Gomori's one step trichrome. I have only ever used it for intestinal protozoa but I think it would work for all. regards Tony Tony Reilly B.App.Sc. , M.Sc. Chief Scientist, Anatomical Pathology Pathology Queensland-PA Laboratory ________________________________________________ Health Services Support Agency | Department of Health Level 1, Building 15,Princess Alexandra Hospital Ipswich Road,WOOLLOONGABBA Qld4102 Ph: 07 3176 2412 Mob: 0402 139411 Fax: 07 3176 2930 Email: tony_reilly@health.qld.gov.au Web: www.health.qld.gov.au/qhcss/ >>> Bob Richmond 2/28/2013 4:19 am >>> Matt Brooks asks: >>One of our Neuropath docs is inquiring about a special stain for unencased [amoebae] in cornea biopsies. I did a search and Gridley's method was the best option that appreaded [sic]. Is there someone that could either verify this stain will work on this organism or let me know what you stain you are using?<< and Ren? Buesa suggests Heidenhain's iron hematoxylin. Corneal ulcers are caused by Acanthamoeba and related species, rather than by Entamoeba histolytica. Google >>amoeba corneal stain<< - some references suggest a fluorescent stain such as calcofluor white, if you have access to a fluorescence microscope. Iron hematoxylins provide exquisite nuclear detail, but are challenging to set up for the first time (I've done it), and are not specific. Periodic acid Schiff (PAS) is often used to demonstrate the glycogen in Entamoeba histolytica trophozoites, but I don't know if the other pathogenic amoebae contain glycogen. My advice would be to ask one of the academic eye pathologists. My choice would be Dr. Hans Grossniklaus at Emory, but there are a good many more. And remember you'll need to find a control slide. Bob Richmond Samurai Pathologist Maryville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ********************************************************************************** From rsrichmond <@t> gmail.com Wed Feb 27 20:12:22 2013 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Wed Feb 27 20:17:22 2013 Subject: [Histonet] Re: Unencased Amoeba Stain In-Reply-To: <512F4677.411C.0039.0@health.qld.gov.au> References: <512F4677.411C.0039.0@health.qld.gov.au> Message-ID: You're referring I suppose to the "Gomori-Wheatley" modification of the one step trichrome, used for staining intestinal amoebae. Don't know if it will work on tissue sections. It has quite a number of variants. Once again, I'd advise consulting an eye pathologist. Bob Richmond Samurai Pathologist Maryville TN ***************** On Wed, Feb 27, 2013 at 8:58 PM, Tony Reilly wrote: > Hi Matt > > The Heidenhain's stain is good but if you want one that is prettier try > Gomori's one step trichrome. I have only ever used it for intestinal > protozoa but I think it would work for all. > > regards > Tony > > Tony Reilly B.App.Sc. , M.Sc. > > Chief Scientist, Anatomical Pathology > > Pathology Queensland-PA Laboratory > > ________________________________________________ > Health Services Support Agency | Department of Health > > Level 1, Building 15,Princess Alexandra Hospital > > Ipswich Road,WOOLLOONGABBA Qld 4102 > Ph: 07 3176 2412 > Mob: 0402 139411 > > Fax: 07 3176 2930 > > Email: tony_reilly@health.qld.gov.au > > Web: www.health.qld.gov.au/qhcss/ > >>>> Bob Richmond 2/28/2013 4:19 am >>> > > Matt Brooks asks: >>One of our Neuropath docs is inquiring about a > special stain for unencased > [amoebae] in cornea biopsies. I did a search and Gridley's method was > the best option that appreaded [sic]. Is there someone that could > either verify this stain will work on this organism or let me know > what you stain you are using?<< and Ren? Buesa suggests Heidenhain's > iron hematoxylin. > > Corneal ulcers are caused by Acanthamoeba and related species, rather > than by Entamoeba histolytica. Google >>amoeba corneal stain<< - some > references suggest a fluorescent stain such as calcofluor white, if > you have access to a fluorescence microscope. > > Iron hematoxylins provide exquisite nuclear detail, but are > challenging to set up for the first time (I've done it), and are not > specific. > > Periodic acid Schiff (PAS) is often used to demonstrate the glycogen > in Entamoeba histolytica trophozoites, but I don't know if the other > pathogenic amoebae contain glycogen. > > My advice would be to ask one of the academic eye pathologists. My > choice would be Dr. Hans Grossniklaus at Emory, but there are a good > many more. And remember you'll need to find a control slide. > > Bob Richmond > Samurai Pathologist > Maryville TN From Tony_Reilly <@t> health.qld.gov.au Wed Feb 27 20:20:29 2013 From: Tony_Reilly <@t> health.qld.gov.au (Tony Reilly) Date: Wed Feb 27 20:21:20 2013 Subject: [Histonet] Re: Unencased Amoeba Stain In-Reply-To: References: <512F4677.411C.0039.0@health.qld.gov.au> Message-ID: <512F4B8D.411C.0039.0@health.qld.gov.au> It is true that I have never used it on tissue sections as I have usually performed it on smears for the Microbiology department. However some of the specimens stained were formalin fixed and stained well while others were PVA fixed. regards Tony Tony Reilly B.App.Sc. , M.Sc. Chief Scientist, Anatomical Pathology Pathology Queensland-PA Laboratory ________________________________________________ Health Services Support Agency | Department of Health Level 1, Building 15,Princess Alexandra Hospital Ipswich Road,WOOLLOONGABBA Qld4102 Ph: 07 3176 2412 Mob: 0402 139411 Fax: 07 3176 2930 Email: tony_reilly@health.qld.gov.au Web: www.health.qld.gov.au/qhcss/ >>> Bob Richmond 2/28/2013 12:12 pm >>> You're referring I suppose to the "Gomori-Wheatley" modification of the one step trichrome, used for staining intestinal amoebae. Don't know if it will work on tissue sections. It has quite a number of variants. Once again, I'd advise consulting an eye pathologist. Bob Richmond Samurai Pathologist Maryville TN ***************** On Wed, Feb 27, 2013 at 8:58 PM, Tony Reilly wrote: > Hi Matt > > The Heidenhain's stain is good but if you want one that is prettier try > Gomori's one step trichrome. I have only ever used it for intestinal > protozoa but I think it would work for all. > > regards > Tony > > Tony Reilly B.App.Sc. , M.Sc. > > Chief Scientist, Anatomical Pathology > > Pathology Queensland-PA Laboratory > > ________________________________________________ > Health Services Support Agency | Department of Health > > Level 1, Building 15,Princess Alexandra Hospital > > Ipswich Road,WOOLLOONGABBA Qld 4102 > Ph: 07 3176 2412 > Mob: 0402 139411 > > Fax: 07 3176 2930 > > Email: tony_reilly@health.qld.gov.au > > Web: www.health.qld.gov.au/qhcss/ > >>>> Bob Richmond 2/28/2013 4:19 am >>> > > Matt Brooks asks: >>One of our Neuropath docs is inquiring about a > special stain for unencased > [amoebae] in cornea biopsies. I did a search and Gridley's method was > the best option that appreaded [sic]. Is there someone that could > either verify this stain will work on this organism or let me know > what you stain you are using?<< and Ren? Buesa suggests Heidenhain's > iron hematoxylin. > > Corneal ulcers are caused by Acanthamoeba and related species, rather > than by Entamoeba histolytica. Google >>amoeba corneal stain<< - some > references suggest a fluorescent stain such as calcofluor white, if > you have access to a fluorescence microscope. > > Iron hematoxylins provide exquisite nuclear detail, but are > challenging to set up for the first time (I've done it), and are not > specific. > > Periodic acid Schiff (PAS) is often used to demonstrate the glycogen > in Entamoeba histolytica trophozoites, but I don't know if the other > pathogenic amoebae contain glycogen. > > My advice would be to ask one of the academic eye pathologists. My > choice would be Dr. Hans Grossniklaus at Emory, but there are a good > many more. And remember you'll need to find a control slide. > > Bob Richmond > Samurai Pathologist > Maryville TN ******************************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ********************************************************************************** From rsrichmond <@t> gmail.com Wed Feb 27 20:34:22 2013 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Wed Feb 27 20:42:34 2013 Subject: [Histonet] Re: Unencased Amoeba Stain In-Reply-To: <512F4B8D.411C.0039.0@health.qld.gov.au> References: <512F4677.411C.0039.0@health.qld.gov.au> <512F4B8D.411C.0039.0@health.qld.gov.au> Message-ID: It's better not to assume that a stain developed for smears will work on tissue sections without modification. The Gram stain is an obvious case in point. Bob Richmond Samurai Pathologist Maryville TN ********************************************* On Wed, Feb 27, 2013 at 9:20 PM, Tony Reilly wrote: > It is true that I have never used it on tissue sections as I have usually > performed it on smears for the Microbiology department. However some of > the specimens stained were formalin fixed and stained well while others were > PVA fixed. > > regards > Tony > > > > > Tony Reilly B.App.Sc. , M.Sc. > > Chief Scientist, Anatomical Pathology > > Pathology Queensland-PA Laboratory > > ________________________________________________ > Health Services Support Agency | Department of Health > > > > Level 1, Building 15,Princess Alexandra Hospital > > Ipswich Road,WOOLLOONGABBA Qld 4102 > Ph: 07 3176 2412 > Mob: 0402 139411 > > Fax: 07 3176 2930 > > Email: tony_reilly@health.qld.gov.au > > Web: www.health.qld.gov.au/qhcss/ > > > > > > > >>>> Bob Richmond 2/28/2013 12:12 pm >>> > > You're referring I suppose to the "Gomori-Wheatley" modification of > the one step trichrome, used for staining intestinal amoebae. Don't > know if it will work on tissue sections. It has quite a number of > variants. > > Once again, I'd advise consulting an eye pathologist. > > Bob Richmond > Samurai Pathologist > Maryville TN > ***************** > On Wed, Feb 27, 2013 at 8:58 PM, Tony Reilly > wrote: >> Hi Matt >> >> The Heidenhain's stain is good but if you want one that is prettier try >> Gomori's one step trichrome. I have only ever used it for intestinal >> protozoa but I think it would work for all. >> >> regards >> Tony >> >> Tony Reilly B.App.Sc. , M.Sc. >> >> Chief Scientist, Anatomical Pathology >> >> Pathology Queensland-PA Laboratory >> >> ________________________________________________ >> Health Services Support Agency | Department of Health >> >> Level 1, Building 15,Princess Alexandra Hospital >> >> Ipswich Road,WOOLLOONGABBA Qld 4102 >> Ph: 07 3176 2412 >> Mob: 0402 139411 >> >> Fax: 07 3176 2930 >> >> Email: tony_reilly@health.qld.gov.au >> >> Web: www.health.qld.gov.au/qhcss/ >> >>>>> Bob Richmond 2/28/2013 4:19 am >>> >> >> Matt Brooks asks: >>One of our Neuropath docs is inquiring about a >> special stain for unencased >> [amoebae] in cornea biopsies. I did a search and Gridley's method was >> the best option that appreaded [sic]. Is there someone that could >> either verify this stain will work on this organism or let me know >> what you stain you are using?<< and Ren? Buesa suggests Heidenhain's >> iron hematoxylin. >> >> Corneal ulcers are caused by Acanthamoeba and related species, rather >> than by Entamoeba histolytica. Google >>amoeba corneal stain<< - some >> references suggest a fluorescent stain such as calcofluor white, if >> you have access to a fluorescence microscope. >> >> Iron hematoxylins provide exquisite nuclear detail, but are >> challenging to set up for the first time (I've done it), and are not >> specific. >> >> Periodic acid Schiff (PAS) is often used to demonstrate the glycogen >> in Entamoeba histolytica trophozoites, but I don't know if the other >> pathogenic amoebae contain glycogen. >> >> My advice would be to ask one of the academic eye pathologists. My >> choice would be Dr. Hans Grossniklaus at Emory, but there are a good >> many more. And remember you'll need to find a control slide. >> >> Bob Richmond >> Samurai Pathologist >> Maryville TN > > ******************************************************************************** > > This email, including any attachments sent with it, is confidential and for > the sole use of the intended recipient(s). This confidentiality is not > waived or lost, if you receive it and you are not the intended recipient(s), > or if it is transmitted/received in error. > > Any unauthorised use, alteration, disclosure, distribution or review of this > email is strictly prohibited. The information contained in this email, > including any attachment sent with it, may be subject to a statutory duty of > confidentiality if it relates to health service matters. > > If you are not the intended recipient(s), or if you have received this email > in error, you are asked to immediately notify the sender by telephone > collect on Australia +61 1800 198 175 or by return email. You should also > delete this email, and any copies, from your computer system network and > destroy any hard copies produced. > > If not an intended recipient of this email, you must not copy, distribute or > take any action(s) that relies on it; any form of disclosure, modification, > distribution and/or publication of this email is also prohibited. > > Although Queensland Health takes all reasonable steps to ensure this email > does not contain malicious software, Queensland Health does not accept > responsibility for the consequences if any person's computer inadvertently > suffers any disruption to services, loss of information, harm or is infected > with a virus, other malicious computer programme or code that may occur as a > consequence of receiving this email. > > Unless stated otherwise, this email represents only the views of the sender > and not the views of the Queensland Government. > > ********************************************************************************** > > From lwhite38 <@t> cogeco.ca Thu Feb 28 05:33:36 2013 From: lwhite38 <@t> cogeco.ca (Lori White) Date: Thu Feb 28 05:33:43 2013 Subject: [Histonet] RE: Waste Alcohol In-Reply-To: <0MIU00NEP8VB1N41@busymta03.int.cogeco.net> References: <0MIU00NEP8VB1N41@busymta03.int.cogeco.net> Message-ID: <000e01ce15a7$725c33d0$57149b70$@ca> Our alcohols that cannot be recycled (contaminated with xylene) are combined with other flammable waste and dealt with by an external company. L.White -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Tuesday, February 26, 2013 12:36 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 111, Issue 37 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Maxvison anti-Rabbit HRP Antibody Substitution (Suresch, Donna L.) 2. RE: Maxvison anti-Rabbit HRP Antibody Substitution (Sarah Dysart) 3. CAP checklist question (Hannen, Valerie) 4. Ventana Labels (Matthew Roark) 5. Re: Ventana Labels (Mark Tarango) 6. RE: Ventana Labels (Bitting, Angela K.) 7. IgG4 (Susan Foreman) 8. Streaking when Cutting (nmhisto@comcast.net) 9. Waste Alcohol (Scott, Allison D) 10. RE: Waste Alcohol (Rathborne, Toni) 11. RE: Waste Alcohol (Horn, Hazel V) 12. RE: Waste Alcohol (Mike Pence) 13. Re: Waste Alcohol (Rene J Buesa) 14. Dako autostainer service (Karla J. C.) 15. RE: CAP checklist question (Susan.Walzer@HCAHealthcare.com) 16. Question for Alkaline Phosphatase / Biotin System Users (Amy Porter) 17. RE: Waste Alcohol (Horn, Hazel V) 18. RE: Waste Alcohol (Podawiltz, Thomas) 19. Re: Waste Alcohol (Brendal Finlay) 20. streaks (Sheila Fonner) ---------------------------------------------------------------------- Message: 1 Date: Mon, 25 Feb 2013 13:07:02 -0500 From: "Suresch, Donna L." Subject: [Histonet] Maxvison anti-Rabbit HRP Antibody Substitution To: "histonet@lists.utsouthwestern.edu" Message-ID: <2C9A1D9608959940943F357E0A470FF8B2A9D0BC46@USCTMXP51005.merck.com> Content-Type: text/plain; charset="us-ascii" Hello All, I have been using Maxvision anti-Rabbit HRP secondary antibody with excellent results. The product has been discontinued. Has anyone found a good substitution for this antibody? Thank you. Donna L. Suresch Senior Imaging Research Scientist Merck Research Laboratories Department of Imaging - West Point Campus Mail Stop: WP44K Office: WP44-H129 770 Sumneytown Pike PO Box 4 West Point, PA 19486-0004 Phone: 215-652-7349 Fax: 215-993-6803 Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------ Message: 2 Date: Mon, 25 Feb 2013 18:15:20 +0000 From: Sarah Dysart Subject: [Histonet] RE: Maxvison anti-Rabbit HRP Antibody Substitution To: "Suresch, Donna L." , "histonet@lists.utsouthwestern.edu" Message-ID: <8A70A9B2ECDD084DACFE6C59FCF86D50485058B1@BL2PRD0711MB434.namprd07.prod.outl ook.com> Content-Type: text/plain; charset="us-ascii" The Biocare polymers (MACH2 series) are excellent!! Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Suresch, Donna L. Sent: Monday, February 25, 2013 12:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Maxvison anti-Rabbit HRP Antibody Substitution Hello All, I have been using Maxvision anti-Rabbit HRP secondary antibody with excellent results. The product has been discontinued. Has anyone found a good substitution for this antibody? Thank you. Donna L. Suresch Senior Imaging Research Scientist Merck Research Laboratories Department of Imaging - West Point Campus Mail Stop: WP44K Office: WP44-H129 770 Sumneytown Pike PO Box 4 West Point, PA 19486-0004 Phone: 215-652-7349 Fax: 215-993-6803 Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Mon, 25 Feb 2013 13:21:16 -0500 From: "Hannen, Valerie" Subject: [Histonet] CAP checklist question To: "histonet@lists.utsouthwestern.edu" Message-ID: <450B7A81EDA0C54E97C53D60F00776C3231B3D7BEC@isexstore03> Content-Type: text/plain; charset="us-ascii" Hi gang.. I am working on a self-inspection CAP checklist and came across the following: ANP.23120 Tissue Processor Tissue processing schedules are validated. Note: New tissue processing schedules must be validated against the standard laboratory processing schedule. Evidence of compliance: Written procedure for validation of new tissue processing schedules AND WC records documenting validation What do you all make of this?? We have not had any new processing schedules introduced into our lab in years. So, I really don't know how to proceed on this one. Any insite will be greatly appreciated. Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.hannen@parrishmed.com =================== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" =================== ------------------------------ Message: 4 Date: Mon, 25 Feb 2013 13:33:55 -0600 From: "Matthew Roark" Subject: [Histonet] Ventana Labels To: Message-ID: <002101ce138f$0cf92a30$26eb7e90$@net> Content-Type: text/plain; charset="us-ascii" Hello all, I am getting ready to try some slide labels as an alternative to the Ventana labels. I was wondering if anyone else is using them or have tried them in the past. They do not have that plastic fold over flap and are suppose to be quite economical, though I have not got a quote yet. They are called FloProTek (http://www.easternlabsvc.com/floprotek.php) - Mercedes Medical Supply sent me the trial roll. Thanks! Matthew Roark- HT/HTL(ASCP)CM Histology Specialist Saint Francis Medical Center 211 Saint Francis Drive Cape Girardeau, MO 63703 573-331-5267 mroark@sfmc.net http://www.sfmc.net ------------------------------ Message: 5 Date: Mon, 25 Feb 2013 11:43:38 -0800 From: Mark Tarango Subject: Re: [Histonet] Ventana Labels To: Matthew Roark Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hi Matthew, We use them and they work fine. They are much cheaper too. We've had them for about 3 years. I initially had some concerns about the DAB being absorbed and staining the labels on some cases. Our safety person told me that it's not a safety concern and it's safe to touch the label after it's stained brown by DAB. This doesn't happen on every slide but is a random thing. It might have something to do with how well it was affixed to the slide. The labels don't affect the staining in my experience. Mark On Mon, Feb 25, 2013 at 11:33 AM, Matthew Roark wrote: > Hello all, > > I am getting ready to try some slide labels as an alternative to the > Ventana labels. I was wondering if anyone else is using them or have > tried them in the past. > > They do not have that plastic fold over flap and are suppose to be > quite economical, though I have not got a quote yet. > > They are called FloProTek (http://www.easternlabsvc.com/floprotek.php) > - Mercedes Medical Supply sent me the trial roll. > > Thanks! > > > Matthew Roark- HT/HTL(ASCP)CM > Histology Specialist > Saint Francis Medical Center > 211 Saint Francis Drive > Cape Girardeau, MO 63703 > 573-331-5267 > mroark@sfmc.net > http://www.sfmc.net > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 6 Date: Mon, 25 Feb 2013 19:55:59 +0000 From: "Bitting, Angela K." Subject: RE: [Histonet] Ventana Labels To: Matthew Roark , "histonet@lists.utsouthwestern.edu" Message-ID: <77F52EFAB8B1694B885E277C48FCD0F6328503A5@GHSEXMBX4W8K1V.geisinger.edu> Content-Type: text/plain; charset="us-ascii" I just tried them Friday. They worked fine but the label picked up counterstain a bit. However, depending on the cost per label, it may be worth the trouble. I'm waiting for a quote. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Matthew Roark Sent: Monday, February 25, 2013 2:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana Labels Hello all, I am getting ready to try some slide labels as an alternative to the Ventana labels. I was wondering if anyone else is using them or have tried them in the past. They do not have that plastic fold over flap and are suppose to be quite economical, though I have not got a quote yet. They are called FloProTek (http://www.easternlabsvc.com/floprotek.php) - Mercedes Medical Supply sent me the trial roll. Thanks! Matthew Roark- HT/HTL(ASCP)CM Histology Specialist Saint Francis Medical Center 211 Saint Francis Drive Cape Girardeau, MO 63703 573-331-5267 mroark@sfmc.net http://www.sfmc.net _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. ------------------------------------------------------------------------- This message was secured by ZixCorp(R). ------------------------------ Message: 7 Date: Mon, 25 Feb 2013 14:57:25 -0500 From: "Susan Foreman" Subject: [Histonet] IgG4 To: Message-ID: <002e01ce1392$554fa900$ffeefb00$@com> Content-Type: text/plain; charset="us-ascii" Has anyone worked up IgG4 on the Ventana Benchmark Ultra? What vendor? How about running it on fresh frozen sections? FITC? Vendor? Many Thanks for your input, Susan ------------------------------ Message: 8 Date: Mon, 25 Feb 2013 20:09:08 +0000 (UTC) From: nmhisto@comcast.net Subject: [Histonet] Streaking when Cutting To: HISTONET Message-ID: <1822368949.1502216.1361822948867.JavaMail.root@sz0075a.emeryville.ca.mail.c omcast.net> Content-Type: text/plain; charset=utf-8 I've been quietly observing from the sidelines, but I just can't pass this one up.B One should never streak when cutting - it's distracting to fellow techs.B Okay - back to my nap. ------------------------------ Message: 9 Date: Mon, 25 Feb 2013 20:27:55 +0000 From: "Scott, Allison D" Subject: [Histonet] Waste Alcohol To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Hello to all in histoland. Is there anyone putting alcohol into a waste drum like for xylene. We have a company that takes away our xylene. Now the safety people are suggesting that we treat the alcohol the same way. We have always poured the alcohol down the drain. It seems very expensive. Your help in this matter will be greatly appreciated. Allison Scott HT(ASCP) Supervisor, Histology Lab LBJ Hospital Harris Health System Office: 713-566-5287 Lab: 713-566-5287 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. ------------------------------ Message: 10 Date: Mon, 25 Feb 2013 20:33:27 +0000 From: "Rathborne, Toni" Subject: [Histonet] RE: Waste Alcohol To: "'Scott, Allison D'" , "histonet@lists.utsouthwestern.edu" Message-ID: <3AD061FE740D464FAC7BF6B5CFB75707599DF5C3@smcmail02.somerset-healthcare.com> Content-Type: text/plain; charset="us-ascii" We have been disposing of our alcohols like this for years. This would include alcoholic eosin too. We are allowed to mix the waste xylene with the alcohols because they are the same classification, and treated the same way. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Monday, February 25, 2013 3:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Waste Alcohol Hello to all in histoland. Is there anyone putting alcohol into a waste drum like for xylene. We have a company that takes away our xylene. Now the safety people are suggesting that we treat the alcohol the same way. We have always poured the alcohol down the drain. It seems very expensive. Your help in this matter will be greatly appreciated. Allison Scott HT(ASCP) Supervisor, Histology Lab LBJ Hospital Harris Health System Office: 713-566-5287 Lab: 713-566-5287 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Mon, 25 Feb 2013 15:02:10 -0600 From: "Horn, Hazel V" Subject: [Histonet] RE: Waste Alcohol To: "'Scott, Allison D'" , "histonet@lists.utsouthwestern.edu" Message-ID: <25A4DE08332B19499904459F00AAACB719BE135634@EVS1.archildrens.org> Content-Type: text/plain; charset="us-ascii" Our xylene and alcohol go into a waste drum to be disposed of. We do recycle both but of course we still have waste to dispose of. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Monday, February 25, 2013 2:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Waste Alcohol Hello to all in histoland. Is there anyone putting alcohol into a waste drum like for xylene. We have a company that takes away our xylene. Now the safety people are suggesting that we treat the alcohol the same way. We have always poured the alcohol down the drain. It seems very expensive. Your help in this matter will be greatly appreciated. Allison Scott HT(ASCP) Supervisor, Histology Lab LBJ Hospital Harris Health System Office: 713-566-5287 Lab: 713-566-5287 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** ********************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ------------------------------ Message: 12 Date: Mon, 25 Feb 2013 21:18:12 +0000 From: Mike Pence Subject: [Histonet] RE: Waste Alcohol To: "Scott, Allison D" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" You should be recycling both xylene and alcohol. Cost savings compared to disposal cost will pay for a recycler in 3-5 years. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Scott, Allison D [Allison.Scott@harrishealth.org] Sent: Monday, February 25, 2013 2:27 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Waste Alcohol Hello to all in histoland. Is there anyone putting alcohol into a waste drum like for xylene. We have a company that takes away our xylene. Now the safety people are suggesting that we treat the alcohol the same way. We have always poured the alcohol down the drain. It seems very expensive. Your help in this matter will be greatly appreciated. Allison Scott HT(ASCP) Supervisor, Histology Lab LBJ Hospital Harris Health System Office: 713-566-5287 Lab: 713-566-5287 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 13 Date: Mon, 25 Feb 2013 13:53:22 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] Waste Alcohol To: "Scott, Allison D" , "histonet@lists.utsouthwestern.edu" Message-ID: <1361829202.27805.YahooMailNeo@web163104.mail.bf1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I always dumped the ethanol down the drain. I never recycled it because it takes almost 3 times more time that recycling xylene (3 times the cost) and the recycled alcohol is seldom of good quality. When I stopped using xylene I had no more use (and exposure) when recycling. Reni J. From: "Scott, Allison D" To: "histonet@lists.utsouthwestern.edu" Sent: Monday, February 25, 2013 3:27 PM Subject: [Histonet] Waste Alcohol Hello to all in histoland. Is there anyone putting alcohol into a waste drum like for xylene. We have a company that takes away our xylene. Now the safety people are suggesting that we treat the alcohol the same way. We have always poured the alcohol down the drain. It seems very expensive. Your help in this matter will be greatly appreciated. Allison Scott HT(ASCP) Supervisor, Histology Lab LBJ Hospital Harris Health System Office: 713-566-5287 Lab: 713-566-5287 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Mon, 25 Feb 2013 13:47:34 -0800 From: "Karla J. C." Subject: [Histonet] Dako autostainer service To: Message-ID: <000f01ce13a1$b8de93a0$2a9bbae0$@dermatopathologynorthwest.com> Content-Type: text/plain; charset="us-ascii" Hi All, Help.Dako just recently informed us that they would no longer provide a Service contract for our immuno autostainer. Is anyone else experiencing this? Does anyone know of any other service people that may be able to work on this equipment (model 3400 year 2003)? We are located in Seattle. Thanks, Karla Carlmas Dermatopathology Northwest ------------------------------ Message: 15 Date: Tue, 26 Feb 2013 01:55:58 -0600 From: Subject: [Histonet] RE: CAP checklist question To: , Message-ID: <4BF03F5404EBDE409AF9232DA74B9DED2DFF10BF41@FWDCWPMSGCMS09.hca.corpad.net> Content-Type: text/plain; charset="us-ascii" We put in a procedure stating that should we change the schedule we will test a variety of tissue (10 different types) and have the pathologist assess the run. We have not changed a schedule in years but we seek to comply... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie Sent: Monday, February 25, 2013 1:21 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP checklist question Hi gang.. I am working on a self-inspection CAP checklist and came across the following: ANP.23120 Tissue Processor Tissue processing schedules are validated. Note: New tissue processing schedules must be validated against the standard laboratory processing schedule. Evidence of compliance: Written procedure for validation of new tissue processing schedules AND WC records documenting validation What do you all make of this?? We have not had any new processing schedules introduced into our lab in years. So, I really don't know how to proceed on this one. Any insite will be greatly appreciated. Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.hannen@parrishmed.com ============= "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ============= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 16 Date: Tue, 26 Feb 2013 09:14:28 -0500 From: "Amy Porter" Subject: [Histonet] Question for Alkaline Phosphatase / Biotin System Users To: Message-ID: <000d01ce142b$96d73ac0$c485b040$@edu> Content-Type: text/plain; charset="us-ascii" We are using standard avidin / biotin staining with alkaline phosphatase labeling and sometimes, not every time and not every slide in the same run we get residual blotchy spots and streaking of the reaction on and off the tissue. We have increased to two rinses after primary and two rinses after alk phos reagent prior to substrate; still happening on some slides...anyone else have this experience or type of problem? Any experiences or suggestions are appreciated. Just can't figure out what is causing it.. Amy S. Porter, HT(ASCP) QIHC Michigan State University Investigative HistoPathology Laboratory portera@msu.edu ------------------------------ Message: 17 Date: Tue, 26 Feb 2013 09:27:03 -0600 From: "Horn, Hazel V" Subject: RE: [Histonet] Waste Alcohol To: "'Rene J Buesa'" , "Scott, Allison D" , "histonet@lists.utsouthwestern.edu" Message-ID: <25A4DE08332B19499904459F00AAACB719BE13563A@EVS1.archildrens.org> Content-Type: text/plain; charset="iso-8859-1" The ability to put alcohol down the drain is regulated by your wastewater utility. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Monday, February 25, 2013 3:53 PM To: Scott, Allison D; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Waste Alcohol I always dumped the ethanol down the drain. I never recycled it because it takes almost 3 times more time that recycling xylene (3 times the cost) and the recycled alcohol is seldom of good quality. When I stopped using xylene I had no more use (and exposure) when recycling. Reni J. From: "Scott, Allison D" To: "histonet@lists.utsouthwestern.edu" Sent: Monday, February 25, 2013 3:27 PM Subject: [Histonet] Waste Alcohol Hello to all in histoland. Is there anyone putting alcohol into a waste drum like for xylene. We have a company that takes away our xylene. Now the safety people are suggesting that we treat the alcohol the same way. We have always poured the alcohol down the drain. It seems very expensive. Your help in this matter will be greatly appreciated. Allison Scott HT(ASCP) Supervisor, Histology Lab LBJ Hospital Harris Health System Office: 713-566-5287 Lab: 713-566-5287 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** ********************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ------------------------------ Message: 18 Date: Tue, 26 Feb 2013 10:55:14 -0500 From: "Podawiltz, Thomas" Subject: RE: [Histonet] Waste Alcohol To: "Horn, Hazel V" , 'Rene J Buesa' , "Scott, Allison D" , "histonet@lists.utsouthwestern.edu" Message-ID: <38667E7FB77ECD4E91BFAEB8D986386324FCF3C6D9@LRGHEXVS1.practice.lrgh.org> Content-Type: text/plain; charset="iso-8859-1" Reagent Alcohol contains methanol, which is a U-listed EPA hazardous chemical and cannot be poured down the drain. Tom Podawiltz HT (ASCP) Histology Section Head/Laboratory Safety Officer. LRGHealthcare Laconia, NH 03246 603-524-3211 ext: 3220 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Tuesday, February 26, 2013 10:27 AM To: 'Rene J Buesa'; Scott, Allison D; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Waste Alcohol The ability to put alcohol down the drain is regulated by your wastewater utility. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Monday, February 25, 2013 3:53 PM To: Scott, Allison D; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Waste Alcohol I always dumped the ethanol down the drain. I never recycled it because it takes almost 3 times more time that recycling xylene (3 times the cost) and the recycled alcohol is seldom of good quality. When I stopped using xylene I had no more use (and exposure) when recycling. Reni J. From: "Scott, Allison D" To: "histonet@lists.utsouthwestern.edu" Sent: Monday, February 25, 2013 3:27 PM Subject: [Histonet] Waste Alcohol Hello to all in histoland. Is there anyone putting alcohol into a waste drum like for xylene. We have a company that takes away our xylene. Now the safety people are suggesting that we treat the alcohol the same way. We have always poured the alcohol down the drain. It seems very expensive. Your help in this matter will be greatly appreciated. Allison Scott HT(ASCP) Supervisor, Histology Lab LBJ Hospital Harris Health System Office: 713-566-5287 Lab: 713-566-5287 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** ********************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. 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Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. ------------------------------ Message: 19 Date: Tue, 26 Feb 2013 09:56:12 -0600 From: "Brendal Finlay" Subject: Re: [Histonet] Waste Alcohol To: "Rene J Buesa" , "Scott, Allison D" , histonet@lists.utsouthwestern.edu Message-ID: <4fcdbb6eed8fb2fc1e5ea8e615ce00ee@medicalcenterclinic.com> Content-Type: text/plain; charset="utf-8" YouB will probably want to contact your local or state office of environmental protection to find out what is required in your area.B B The EPA lists hazardous wastes in 40 CFR 261.30.B Even if your waste is not on this list, it may have hazardous characteristics such as: ignitability, corrosivity, reactivity, and toxicity characteristic.B Ignitability pertains to flashpoints less than 140 F and/or aqueous solutions with alcohol content greater than or equal to 24 percent.B B Florida's DEP has different regulations dependent upon the quantity of hazardous waste generated.B Some localities may require approval to dispose of alcohol down the drain.B If you don't have this approval, you have to dispose of the waste according toB your stateB andB federalregulations.B B To reduce the quantity of hazardous waste we generate, we began to recycle using CBG Biotech's distillation unit.B We have had great results with it.B While alcohol does take longer to recycle than xylene, our supply costs have been greatly reduced and we have worked this into our weekly schedule very well. B Some things to consider when using recycled alcohol: Test each finished alcohol run with a hydrometer to know what percentage it is.B Do not use recycled alcohol at the end of the stain line after eosin (it will wash the eosin out). Do not use recycled alcohol as the last alcohol before xylene on the processor. Finally, do not recycle the first alcohols on the stain line after xylene (at least on our recycler). This is disposed of in our flammable waste containers to be picked up. B Hereb From f.morrow <@t> sheffield.ac.uk Thu Feb 28 07:59:52 2013 From: f.morrow <@t> sheffield.ac.uk (Fiona J Morrow) Date: Thu Feb 28 08:00:00 2013 Subject: [Histonet] RESCUING UNDER DECALCIFIED BONE FROM PARAFFIN EMBEDDED BLOCKS Message-ID: Hi Has anyone ever tried to reprocess bone tissue that has been under decalcified and processed through to wax? Thanks Fi M Fiona Morrow Dept. of Infection and Immunity KFloor, Room K118 Medical School University of Sheffield Beech Hill Road Sheffield S10 2RX 0114 271 2102 F.morrow@sheffield.ac.uk From contact <@t> excaliburpathology.com Thu Feb 28 08:27:46 2013 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Thu Feb 28 08:27:52 2013 Subject: [Histonet] Re: Unencased Amoeba Stain In-Reply-To: References: <512F4677.411C.0039.0@health.qld.gov.au> Message-ID: <1362061666.39513.YahooMailNeo@web5709.biz.mail.ne1.yahoo.com> Greetings, once infected tissue is placed in fixative, Acanthamoeba form a double walled encasement that stains nicely with any trichrome stain. Light green works better than aniline blue. The amoeba are large and measure approximately 30 microns. There are about 100 human corneal cases a year in the U.S. and one year back in the 80s we saw 10 of those cases. ? Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. Specializing in Eye Histology 8901 S. Santa Fe, Suite G Oklahoma City, OK 73139 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ________________________________ From: Bob Richmond To: Tony Reilly Cc: histonet@lists.utsouthwestern.edu Sent: Wednesday, February 27, 2013 8:12 PM Subject: Re: [Histonet] Re: Unencased Amoeba Stain You're referring I suppose to the "Gomori-Wheatley" modification of the one step trichrome, used for staining intestinal amoebae. Don't know if it will work on tissue sections. It has quite a number of variants. Once again, I'd advise consulting an eye pathologist. Bob Richmond Samurai Pathologist Maryville TN ***************** On Wed, Feb 27, 2013 at 8:58 PM, Tony Reilly wrote: > Hi Matt > > The Heidenhain's stain is good but if you want one that is prettier try > Gomori's one step trichrome.? I have only ever used it for intestinal > protozoa but I think it would work for all. > > regards > Tony > > Tony Reilly? B.App.Sc. , M.Sc. > > Chief Scientist, Anatomical Pathology > > Pathology Queensland-PA Laboratory > > ________________________________________________ > Health Services Support Agency | Department of Health > > Level 1, Building 15,Princess Alexandra Hospital > > Ipswich Road,WOOLLOONGABBA? Qld 4102 > Ph: 07 3176 2412 > Mob: 0402 139411 > > Fax: 07 3176 2930 > > Email: tony_reilly@health.qld.gov.au > > Web:? www.health.qld.gov.au/qhcss/ > >>>> Bob Richmond 2/28/2013 4:19 am >>> > > Matt Brooks asks: >>One of our Neuropath docs is inquiring about a > special stain for unencased > [amoebae] in cornea biopsies. I did a search and Gridley's method was > the best option that appreaded [sic]. Is there someone that could > either verify this stain will work on this organism or let me know > what you stain you are using?<< and Ren? Buesa suggests Heidenhain's > iron hematoxylin. > > Corneal ulcers are caused by Acanthamoeba and related species, rather > than by Entamoeba histolytica. Google >>amoeba corneal stain<< - some > references suggest a fluorescent stain such as calcofluor white, if > you have access to a fluorescence microscope. > > Iron hematoxylins provide exquisite nuclear detail, but are > challenging to set up for the first time (I've done it), and are not > specific. > > Periodic acid Schiff (PAS) is often used to demonstrate the glycogen > in Entamoeba histolytica trophozoites, but I don't know if the other > pathogenic amoebae contain glycogen. > > My advice would be to ask one of the academic eye pathologists. My > choice would be Dr. Hans Grossniklaus at Emory, but there are a good > many more. And remember you'll need to find a control slide. > > Bob Richmond > Samurai Pathologist > Maryville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Feb 28 09:27:55 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Feb 28 09:27:59 2013 Subject: [Histonet] RESCUING UNDER DECALCIFIED BONE FROM PARAFFIN EMBEDDED BLOCKS In-Reply-To: References: Message-ID: <1362065275.73956.YahooMailNeo@web163106.mail.bf1.yahoo.com> Go back to water (xylene or whatever "clearant" you use ? graded alcohols ? water) and place in decal. The results will be slightly of less quality than if the tissue was correctly decalled in the first place, but you will be able to section it. Ren? J.? From: Fiona J Morrow To: histonet@lists.utsouthwestern.edu Sent: Thursday, February 28, 2013 8:59 AM Subject: [Histonet] RESCUING UNDER DECALCIFIED BONE FROM PARAFFIN EMBEDDED BLOCKS Hi Has anyone ever tried to reprocess bone tissue that has been under decalcified and processed through to wax? Thanks Fi M Fiona Morrow Dept. of Infection and Immunity KFloor, Room K118 Medical School University of Sheffield Beech Hill Road Sheffield S10 2RX 0114 271 2102 F.morrow@sheffield.ac.uk _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PMonfils <@t> Lifespan.org Thu Feb 28 10:41:51 2013 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Thu Feb 28 10:42:29 2013 Subject: [Histonet] Unencased Ameoba Stain In-Reply-To: References: Message-ID: Either Gridley's method or an ordinary PAS will work very well, due to the high concentration of glycogen in the amoebic cytoplasm. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joseph Brooks Sent: Wednesday, February 27, 2013 11:13 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Unencased Ameoba Stain Hello All, One of our Neuropath docs is inquiring about a special stain for unencased ameobas in cornea biopsies. I did a search and Gridley's Method was the best option that appreaded. Is there someone that could either verify this stain will work on this organism or let me know what you stain you are using? Thanks in advance. Matt Brooks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From peter.craven <@t> nhs.net Thu Feb 28 10:46:41 2013 From: peter.craven <@t> nhs.net (Craven Peter (NHS HIGHLAND)) Date: Thu Feb 28 10:54:23 2013 Subject: [Histonet] RESCUING UNDER DECALCIFIED BONE FROM PARAFFIN EMBEDDED BLOCKS In-Reply-To: <20130228140103.1017B478596@nhs-pd1e-esg003.ad1.nhs.net> References: <20130228140103.1017B478596@nhs-pd1e-esg003.ad1.nhs.net> Message-ID: <20130228164933.F196147805E@nhs-pd1e-esg108.ad1.nhs.net> Fiona We have done this in the past we took the blocks back to water be melting them out, re-cassetteing and putting through a cleaning cycle on the excelsior processor then just decal as normal and reprocess, some hardening often occurs but we can get sections of them suitable for reporting. Peter Peter L Craven FIBMS Pathology Department Raigmore Hospital Old Perth Road Inverness IV2 3UJ Tel 01463 704269 email peter.craven@nhs.net ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fiona J Morrow [f.morrow@sheffield.ac.uk] Sent: 28 February 2013 01:59 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RESCUING UNDER DECALCIFIED BONE FROM PARAFFIN EMBEDDED BLOCKS Hi Has anyone ever tried to reprocess bone tissue that has been under decalcified and processed through to wax? Thanks Fi M Fiona Morrow Dept. of Infection and Immunity KFloor, Room K118 Medical School University of Sheffield Beech Hill Road Sheffield S10 2RX 0114 271 2102 F.morrow@sheffield.ac.uk _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************************************************************************************************************** This message may contain confidential information. If you are not the intended recipient please inform the sender that you have received the message in error before deleting it. Please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Thank you for your co-operation. NHSmail is the secure email and directory service available for all NHS staff in England and Scotland NHSmail is approved for exchanging patient data and other sensitive information with NHSmail and GSi recipients NHSmail provides an email address for your career in the NHS and can be accessed anywhere ******************************************************************************************************************** From jpiche-grocki <@t> wtbyhosp.org Thu Feb 28 14:26:30 2013 From: jpiche-grocki <@t> wtbyhosp.org (PicheGrocki, Jessica) Date: Thu Feb 28 14:26:37 2013 Subject: [Histonet] FNA Clia Guidelines Message-ID: <631955447A364B45B9458D2905635110655D26B9@WIN08-MBX-01.wtbyhosp.org> Hi All, Quick question............what are the Clia requirements for Fine needle aspirate procedures? Is it considered high complexity testing? And who prepares the slides when the needle is handed off? Thank you, Jessica Piche, HT(ASCP) CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital From HornHV <@t> archildrens.org Thu Feb 28 15:05:35 2013 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Thu Feb 28 15:05:49 2013 Subject: [Histonet] RE: FNA Clia Guidelines In-Reply-To: <631955447A364B45B9458D2905635110655D26B9@WIN08-MBX-01.wtbyhosp.org> References: <631955447A364B45B9458D2905635110655D26B9@WIN08-MBX-01.wtbyhosp.org> Message-ID: <25A4DE08332B19499904459F00AAACB719BE13564F@EVS1.archildrens.org> Our pathologists do it all. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of PicheGrocki, Jessica Sent: Thursday, February 28, 2013 2:27 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FNA Clia Guidelines Hi All, Quick question............what are the Clia requirements for Fine needle aspirate procedures? Is it considered high complexity testing? And who prepares the slides when the needle is handed off? Thank you, Jessica Piche, HT(ASCP) CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From BZIMMERM <@t> gru.edu Thu Feb 28 15:41:34 2013 From: BZIMMERM <@t> gru.edu (Zimmerman, Billie) Date: Thu Feb 28 15:41:42 2013 Subject: [Histonet] GSH Symposium April 12-14 Message-ID: <7B3DEB32E69C034EACB479059C5DE3FF758BF9@EX-MLB-03.ad.georgiahealth.edu> Don't forget to register for the Georgia Society of Histotechnology meeting April 12-14. What a great getaway weekend to earn up to 15 CEU's from the NSH. Join us for education, networking, and some great scenery! Wanda Simons President of GSH /bz Augusta State University and Georgia Health Sciences University have consolidated to become Georgia Regents University. Effective January 9, 2013, my email address has changed to BZIMMERM@gru.edu. Please update your address book to reflect this change. From jerry.santiago <@t> bellsouth.net Thu Feb 28 19:51:06 2013 From: jerry.santiago <@t> bellsouth.net (Jerry Santiago, MSEd, HTL (ASCP) QIHC) Date: Thu Feb 28 19:56:31 2013 Subject: [Histonet] FSH abstracts deadline Message-ID: Dear histonetters, March 1st is the ddeadline to submit any abstracts consideration for the 2013 Florida Society for Histotechnology meeting. To submit an abstract go to www.fshgroup.org Or email to fsh@fshgroup.org Jerry Santiago FSH