From leila.etemadi <@t> med.lu.se Mon Dec 2 03:44:51 2013 From: leila.etemadi <@t> med.lu.se (Leila Etemadi) Date: Mon Dec 2 03:44:58 2013 Subject: [Histonet] rat's hind paw skin Message-ID: <17C4C4E7-B1A8-4D51-95AC-218FE8737291@med.lu.se> Hello every body, First I would like to thank you all for your great feedbacks and attention which is such a big improvement in my work, Thank you all! Then, I was wonder if any one has experience on this tissue: rat hind paw skin ( which is hairless). Any inputs will be appreciated, whether is about any specific source where I can find technical information about fixation procedure..., or about sectioning,staining procedure? I am looking for melanin pigments and have no clue if it is possible to see it there or not?!? Thanks in advance, Cheers Leila From Beth.Fye <@t> HCAhealthcare.com Mon Dec 2 09:18:47 2013 From: Beth.Fye <@t> HCAhealthcare.com (Beth.Fye@HCAhealthcare.com) Date: Mon Dec 2 09:19:55 2013 Subject: [Histonet] Labeling Error Policies Message-ID: <938F8EC5A524D34EB5796E23E52781D361CC482AAE@NADCWPMSGCMS05.hca.corpad.net> Our laboratories are currently working on policies to address specimen identification/labeling errors. This is to be a laboratory policy and will include pathology. Would anyone be willing to share policies that they have that address this and any punitive action that is mentioned? I know this has been discussed before, and continues to be a hot topic. Thanks in advance for your help regarding this. Beth A. Fye, CT (ASCP) Pathology Technical Manager HCA Richmond Hospital Laboratories office: (804) 228-6564 fax: (877)699-3895 From amurvosh <@t> advancederm.net Mon Dec 2 09:47:17 2013 From: amurvosh <@t> advancederm.net (Anne Murvosh) Date: Mon Dec 2 09:47:24 2013 Subject: [Histonet] Rate of microtome injuries in Histotechnology schools. In-Reply-To: References: Message-ID: <4AD6A4E531E8C943A730559B6B81DF07C89D60@dc.Advancederm.net> If anyone wants to cut down on cutting injuries. Teach the new techs to use two forceps instead of your fingers. I'm too old to change but I know a tech who cuts this way and has never had an injury. It works really well on automated microtomes. Anne -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom Wells Sent: Wednesday, November 27, 2013 12:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Rate of microtome injuries in Histotechnology schools. Hi, I am curious about the rate of microtome cuts that students receive in Histotechnology schools. We have a rather large program and take in approximately 60 (or more) students a year. Our Histotechnology semester is around 20 weeks long. During that time our students cut approximately 300 - 400 blocks per student. We typically have approximately 10 minor cuts per semester. How does this compare to other schools? Please email or call me directly to discuss this further. Thank you very much. Tom Tom Wells BSc, MEd, MLT, ART Faculty Department of Medical Laboratory Sciences, School of Health British Columbia Institute of Technology SW03-3088, 3700 Willingdon Avenue Burnaby, BC, V5G 3H2 E Tom_Wells@bcit.ca T (604) 412-7594 C (778) 228 -4102 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mtighe <@t> trudeauinstitute.org Mon Dec 2 11:06:24 2013 From: mtighe <@t> trudeauinstitute.org (Mike Tighe) Date: Mon Dec 2 11:06:49 2013 Subject: [Histonet] Embedding cells in Paraffin Message-ID: <46d5589df0864870848089e6780fb432@BY2PR07MB469.namprd07.prod.outlook.com> Has anyone tried to embed cells grown in tissue culture? I am trying to put some tissue culture cells through same stress as tissue would go through. Fixation, dehydration, and heat. Any ideas? I could re-suspend in OCT and then fix for extended time with NBF but that doesn't quite seem fair. Thanks for any ideas! From Bauer.Karen <@t> mayo.edu Mon Dec 2 11:08:54 2013 From: Bauer.Karen <@t> mayo.edu (Bauer, Karen L.) Date: Mon Dec 2 11:08:59 2013 Subject: [Histonet] Purple/Blue haze on ISH Message-ID: <8A2A8E67E35BB24DAD9375F9E163107510A7D2@MSGPEXCHA01A.mfad.mfroot.org> Hi all... sending out for a colleague... After implementing the Vantage system, a lot of their ISH slides have a purplish to bluish haze on the slides. Not all of them, but some and it seems to be consistent. No problems before Vantage. They were using the longer labels from Ventana for labeling prior to implementation, but now with Vantage, the slide labels are smaller. That's the only real difference from before and after. Protocols have not changed and they've checked everything else... buffers and such. Any ideas on why this would happen? Thank you, Karen Karen L. Bauer HTL/HT (ASCP) | Histology Supervisor | Pathology | MOHS Lab Supervisor | Dermatology | Phone: 715-838-3205 | bauer.karen@mayo.edu | Mayo Clinic Health System | 1221 Whipple Street | Eau Claire, WI 54702 | mayoclinichealthsystem.org From hfedor <@t> jhmi.edu Mon Dec 2 11:12:54 2013 From: hfedor <@t> jhmi.edu (Helen Fedor) Date: Mon Dec 2 11:12:59 2013 Subject: [Histonet] embedding cells in parrafin #2 Message-ID: Making cell tissue blocks Cells: . Harvest and fix in formalin* for at least 3hrs or overnight (10 fold more formalin) . HAVE A NICE VISIBLE SIZE PELLET (1 confluent T75 minimum or 1 T175) . Remove formalin* and wash 2x's in cold 1x PBS . Cover cells with some 1x PBS and refrigerate Materials for blocks: 1. Toothpick or 20ul pipette tip 2. Agarose (2% solution agarose with 1xPBS) ultra pure agarose cat#15510-027 from core 3. Cells in 1x PBS 4. 0.5ml microtubes (sterile) 5. Tissue cassettes (1 or 6 compartiment cassettes -Electron Microscopy Science 800-523-5874 cat.# 70078-W) 6. New razor blades 7. Clean cutting board 8. Container to submerge cassette into with enough 1x PBS to cover cassettes Method: . Suspend cells in 1xPBS - use just enough to pipette cells (1:1 dilution is MAX) . Make 2% agarose solution - keep in water bath (42C) so doesn't solidify . Make 200ul agarose plug in 0.5ml tube and wait 3 minutes to solidify . Add *120ul of cell solution to .5ml tube and then add *150ul of agarose and pipette up/down ~3 times to mixed well - CUT END OF PIPETTE TIP OFF JUST A LITTLE SO YOU DON'T DESTROY CELL IF NECESSARY . Add toothpick and let sit 5 minutes . Remove plug with toothpick and cut into sections (if necessary) with new blade on clean surface and add to cassette . If you have extra cell plugs then store then in 1x PBS in frig. * Varies according to how much cell suspension you have. ALWAYS add at least 30ul more of agarose in order for it to make a nice plug. IMPORTANT NOTES: . ALWAYS be sure to label your cassette with an experiment number because that's what the slides will be labeled with. . Be SURE to write on BOTH the top of the cassette and down the sides what cell type is in each compartment (if using a 6 compartment cassette) . Tell the histologist what you fixed your cells in - if fixed in formalin, ethanol, paraformaldehyde, etc. - this is vital for the processing/embedding process . ALWAYS put control cell types in the block - +/- . ALWAYS prepare your block in an asymmetric pattern THIS IS A MUST!! or you will not be able to identify which cell type is placed where on the slide Helen L. Fedor Prostate Tissue Bank, Manager Oncology Tissue Services, Manager Johns Hopkins University 600 N. Wolfe St,?| Marburg Room 406 Baltimore, MD?| 21287-7065 410.614.1660 http://tmalab.jhmi.edu/ http://prostatebiorepository.org/ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Tighe Sent: Monday, December 02, 2013 12:06 PM To: histonet@lists.utsouthwestern.edu (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Embedding cells in Paraffin Has anyone tried to embed cells grown in tissue culture? I am trying to put some tissue culture cells through same stress as tissue would go through. Fixation, dehydration, and heat. Any ideas? I could re-suspend in OCT and then fix for extended time with NBF but that doesn't quite seem fair. Thanks for any ideas! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWatson <@t> gnf.org Mon Dec 2 11:19:26 2013 From: JWatson <@t> gnf.org (James Watson) Date: Mon Dec 2 11:19:31 2013 Subject: [Histonet] RE: Embedding cells in Paraffin In-Reply-To: <46d5589df0864870848089e6780fb432@BY2PR07MB469.namprd07.prod.outlook.com> References: <46d5589df0864870848089e6780fb432@BY2PR07MB469.namprd07.prod.outlook.com> Message-ID: We do this all the time. Here is our protocol: Agarose Pre-embedding Cell Cultures Pellets Collected in 15 ml. Tubes Material: ? 10% Neutral Buffer Formalin ? 1X Dulbeccos PBS ? NuSieve GTG Low Melting Point Agarose: Lonza, Cat# 50080 ? Corning 15 ml conical Centrifuge tubes Solutions: 1% Neutral Buffered Formalin 10% Neutral Buffered Formalin??????????????????.10 ml 1X DPBS???????????????..............................................90 ml 3.0% Agarose NuSieve Low Melting Point Agarose powder????????.??3.0 gm 1% Neutral Buffered Formalin?????????????.?..??100.0 ml 1. In a fume hood, warm up the mixture until the agarose completely dissolves and maintain the solution at approximately 40?C. 2. Pipette 1 ml of liquid agarose gel into the 1.5 ml micro-centrifuge tubes. 3. Let solidify and store at room temperature. Procedure for Making Cultured Cell Pellets 1. Culture cells in desired number of vessels (e.g. 3 T-175 flasks) so that you will have 40-60 million cells at appropriate confluence. 2. Cell harvesting: take out part of culture medium and leave 5-10 ml of culture medium in each flask. With the flask placed on ice, quickly scrape off cells using a sterile cell scraper. 3. Spin down the cells at 4?C, 1000 rpm, 5 min. Resuspend cells in cold PBS and then combine cell suspensions from different flasks into one 15 ml tube. (If the total volume of detached cells from all flasks is less than 15 ml, directly transfer cell suspensions to one 15 ml tube. 4. Spin at 1500 rpm, 4?C, for 20 minutes. 5. Carefully discard supernatant without disturbing cell pellet. 6. Along the tube wall, carefully add 4 ml of 10% Neutral Buffered Formalin (NBF) into each tube and incubate at room temperature overnight. 7. Discard 10% NBF without disrupting cell pellet. 8. Carefully add 0.5 ml of 70% ETOH along the tube wall and hold overnight at room temperature. Samples can be stored in 70% ETOH at 4?C at this step for up to 3 days before proceeding. 9. Warm the 3% Agarose to 40?C until completely melted. 10. Remove as much of the 70% ETOH as possible. 11. Pipette on 1.0 ml of the melted Agarose onto the washed cultured cells. 12. Spin down the tube immediately in a centrifuge at 1000-1500 rpm for 2-5min. 13. Make sure that the cultured cells are at the bottom of the tube. 14. Carefully cut the tube slightly above the top of the cell pellet, carefully release the cell pellet into a 35 x 10 mm Tissue Culture Dish containing 3% NuSieve LMP Agarose solution maintained at approximately 40?C. 15. Once agarose completely solidified, bring the pellets to the Histology lab for paraffin embedding. Procedure for processing Cultured Cell Pellets (Histology) 1. Add one or two drops of 70% ETOH to side of well plate to loosen pellet from wall of well. 2. Carefully pop agarose using the modified spatula and wrap in lens paper. 3. Process the pellet on the normal mouse cycle on the tissue processor using the 100% ETOH without the 5% glycerin. 4. Embed the pellet with the cultured cells at the bottom of the mold. James Watson HT? ASCP GNF? Genomics Institute of the Novartis Research Foundation Tel??? 858-332-4647 Fax?? 858-812-1915 jwatson@gnf.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Tighe Sent: Monday, December 02, 2013 9:06 AM To: histonet@lists.utsouthwestern.edu (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Embedding cells in Paraffin Has anyone tried to embed cells grown in tissue culture? I am trying to put some tissue culture cells through same stress as tissue would go through. Fixation, dehydration, and heat. Any ideas? I could re-suspend in OCT and then fix for extended time with NBF but that doesn't quite seem fair. Thanks for any ideas! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CObregon <@t> mhs.net Mon Dec 2 11:43:36 2013 From: CObregon <@t> mhs.net (Obregon, Cecilia) Date: Mon Dec 2 11:43:44 2013 Subject: [Histonet] RE: Purple/Blue haze on ISH In-Reply-To: <8A2A8E67E35BB24DAD9375F9E163107510A7D2@MSGPEXCHA01A.mfad.mfroot.org> References: <8A2A8E67E35BB24DAD9375F9E163107510A7D2@MSGPEXCHA01A.mfad.mfroot.org> Message-ID: <598BB4A3A92D7F4DAA14C30E4AE70C2F27BC904F@MHSEXMB05.mhs.net> Karen, We experienced a similar problem with the ISHs at our laboratory a few months ago with the Kappa, Lambda, Eber and HPV. Our pathologists were complaining of a blue crystal-like precipitate on all ISH slides. Our Ventana Technical Specialist recommended that we try the NO ALCOHOL dehydrating procedure. (The same one we currently use for the Red-Alkaline Phosphatase detection). Once you remove the slides from the Ultra or XT rinse throughly in soapy water and put in the 60 Degrees oven for 15 minutes. Dehydrate in the Symphony using a NO DEHYDRATION coverslip program. We have noticed a signficant decrease in this precipitate using this procedure. Hope if works for you too. Thank you, Cecilia M. Obregon Memorial Regional Hospital 3501 Johnson Street Hollywood, FL 33021 Ph: 954-265-5317 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Bauer, Karen L. [Bauer.Karen@mayo.edu] Sent: Monday, December 02, 2013 12:08 PM To: 'histonet' Subject: [Histonet] Purple/Blue haze on ISH Hi all... sending out for a colleague... After implementing the Vantage system, a lot of their ISH slides have a purplish to bluish haze on the slides. Not all of them, but some and it seems to be consistent. No problems before Vantage. They were using the longer labels from Ventana for labeling prior to implementation, but now with Vantage, the slide labels are smaller. That's the only real difference from before and after. Protocols have not changed and they've checked everything else... buffers and such. Any ideas on why this would happen? Thank you, Karen Karen L. Bauer HTL/HT (ASCP) | Histology Supervisor | Pathology | MOHS Lab Supervisor | Dermatology | Phone: 715-838-3205 | bauer.karen@mayo.edu | Mayo Clinic Health System | 1221 Whipple Street | Eau Claire, WI 54702 | mayoclinichealthsystem.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: DO NOT FORWARD THIS MESSAGE TO OTHERS WITHOUT PERMISSION OF THE SENDER. This e-mail, including any attachments, may contain confidential or privileged material that is exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, dissemination, copying, or taking any action in reliance on its contents is prohibited. If you have any reason to believe this e-mail was not intended for you, please delete the e-mail and any attachments, and notify the sender immediately. From joelleweaver <@t> hotmail.com Mon Dec 2 11:58:11 2013 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Mon Dec 2 11:58:16 2013 Subject: [Histonet] Embedding cells in Paraffin In-Reply-To: <46d5589df0864870848089e6780fb432@BY2PR07MB469.namprd07.prod.outlook.com> References: <46d5589df0864870848089e6780fb432@BY2PR07MB469.namprd07.prod.outlook.com> Message-ID: I spun down the cultured cells and then used a product called histogel. It kept them protected through the VIP, paraffin embedded like cell block "buttons" and was able to cut regular paraffin sections. I believe that I purchased the histogel from American Mastertech. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: mtighe@trudeauinstitute.org > To: histonet@lists.utsouthwestern.edu > Date: Mon, 2 Dec 2013 17:06:24 +0000 > Subject: [Histonet] Embedding cells in Paraffin > > Has anyone tried to embed cells grown in tissue culture? I am trying to put some tissue culture cells through same stress as tissue would go through. Fixation, dehydration, and heat. Any ideas? I could re-suspend in OCT and then fix for extended time with NBF but that doesn't quite seem fair. > > > > Thanks for any ideas! > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Mon Dec 2 12:07:20 2013 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Mon Dec 2 12:07:54 2013 Subject: [Histonet] RE: Embedding cells in Paraffin In-Reply-To: <46d5589df0864870848089e6780fb432@BY2PR07MB469.namprd07.prod.outlook.com> References: <46d5589df0864870848089e6780fb432@BY2PR07MB469.namprd07.prod.outlook.com> Message-ID: <761E2B5697F795489C8710BCC72141FF0B4F96@ex07.net.ucsf.edu> Mike, For EM work we fix, scrape the plate into a microtube, centrifuge at 1800 rpm for 10 min and then embed in Histogel or agar. Processing after that is as usual. They look fine by EM. I'm sure they would look fine by paraffin processing as well. Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Tighe Sent: Monday, December 02, 2013 9:06 AM To: histonet@lists.utsouthwestern.edu (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Embedding cells in Paraffin Has anyone tried to embed cells grown in tissue culture? I am trying to put some tissue culture cells through same stress as tissue would go through. Fixation, dehydration, and heat. Any ideas? I could re-suspend in OCT and then fix for extended time with NBF but that doesn't quite seem fair. Thanks for any ideas! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From astritma <@t> fhcrc.org Mon Dec 2 13:32:01 2013 From: astritma <@t> fhcrc.org (Stritmatter, Andrea N) Date: Mon Dec 2 13:32:09 2013 Subject: [Histonet] Donations Needed Message-ID: <9990B91374C5654E80683908979BDB947A73CF@tigh.fhcrc.org> Dear Histo Folks, I am in the process of setting up a brand new histology/IHC laboratory on the campus of the Uganda Cancer Institute in Kampala, Uganda in conjunction with the Fred Hutchinson Cancer Research Center. As you can imagine, we need anything and everything as supplies and small equipment are extremely hard to obtain in Uganda. If anyone is doing any end-of-year cleaning and would like to donate to a very worthy endeavor, please let me know. We are in need of everything from paraffin pots to weigh boats, beakers to vortex machines, dyes to amber bottles, and forceps to staining jars. Please don't hesitate to contact me with any questions. Thanks so much! Andrea Andrea Stritmatter, HTL (ASCP) QA Manager UCI/Hutchinson Center Cancer Alliance Fred Hutchinson Cancer Research Center 1100 Fairview Ave N, M1-B140 Seattle, WA 98109-1024 Office phone: +1-206-667-2913 Cell phone: +1-206-495-7081 Fax: +1-206-667-1965 Skype address: andrea.stritmatter Email: astritma@fhcrc.org From mtighe <@t> trudeauinstitute.org Mon Dec 2 13:46:33 2013 From: mtighe <@t> trudeauinstitute.org (Mike Tighe) Date: Mon Dec 2 13:46:39 2013 Subject: [Histonet] anti-Human CD20 Message-ID: <629f304c98434024a090b1831a9d0265@BY2PR07MB469.namprd07.prod.outlook.com> Thanks for all the great replies!! While I have you all worried about my cell pellets ;-) Does anyone have a good anti-HuCD20 that works well for FFPE tissues (cells). Thanks! Mike From TNMayer <@t> mdanderson.org Mon Dec 2 15:11:31 2013 From: TNMayer <@t> mdanderson.org (Mayer,Toysha N) Date: Mon Dec 2 15:13:23 2013 Subject: [Histonet] RE: See you Soon Message-ID: <47E9B2C01DDDD94881EACD2DC44EBC8802C035EA@D1PWPEXMBX05.mdanderson.edu> Congratulations Sarah!!!! Sincerely, Toysha N. Mayer, MBA, HT(ASCP) tnmayer@mdanderson.org Instructor/Education Coordinator Program in Histotechnology School of Health Professions MD Anderson Cancer Center 713-563.3481 Message: 1 Date: Wed, 27 Nov 2013 19:13:05 +0000 From: Sarah Dysart Subject: [Histonet] See You Soon To: "histonet@lists.utsouthwestern.edu" Message-ID: <488dd7f2012c47899d4a47bfba5c97e3@BY2PR07MB106.namprd07.prod.outlook.com> Content-Type: text/plain; charset="us-ascii" Hey guys and gals...I have taken a new position as Pathology Supervisor at North Austin Medical Center here in Austin...so I am unsubscribing for now, but have no fear...I will be back ASAP with my new email address. Hope everyone has a great Turkey Day!! Signing off for now... Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ------------------------------ Message: 2 Date: Wed, 27 Nov 2013 11:32:22 -0800 From: Heather Marlatt Subject: Re: [Histonet] See You Soon To: Sarah Dysart Cc: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=ISO-8859-1 CONGRATULATIONS SARAH!!!!! I hope you love the new position! :-) On Wed, Nov 27, 2013 at 11:13 AM, Sarah Dysart wrote: > Hey guys and gals...I have taken a new position as Pathology > Supervisor at North Austin Medical Center here in Austin...so I am > unsubscribing for now, but have no fear...I will be back ASAP with my > new email address. Hope everyone has a great Turkey Day!! > > Signing off for now... > > Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna > Therapeutics > 2150 Woodward Street > Suite 100 > Austin, Texas 78744 > (512)901-0900 ext. 6912 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From amber.mckenzie <@t> gastrodocs.net Mon Dec 2 15:19:51 2013 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Mon Dec 2 15:19:55 2013 Subject: [Histonet] Grossing tables In-Reply-To: <47E9B2C01DDDD94881EACD2DC44EBC8802C035EA@D1PWPEXMBX05.mdanderson.edu> References: <47E9B2C01DDDD94881EACD2DC44EBC8802C035EA@D1PWPEXMBX05.mdanderson.edu> Message-ID: <5A33C952BB67F4468AF1F36D739212BCDD121F7B@JERRY.Gia.com> I'm currently designing a new GI lab. Any advice on grossing stations? The only name I know is Mopec. Is that the best one to go with or are there better ones out there? Thanks! From Joyce.Weems <@t> emoryhealthcare.org Mon Dec 2 15:24:20 2013 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Mon Dec 2 15:24:35 2013 Subject: [Histonet] RE: Grossing tables In-Reply-To: <5A33C952BB67F4468AF1F36D739212BCDD121F7B@JERRY.Gia.com> References: <47E9B2C01DDDD94881EACD2DC44EBC8802C035EA@D1PWPEXMBX05.mdanderson.edu> <5A33C952BB67F4468AF1F36D739212BCDD121F7B@JERRY.Gia.com> Message-ID: I think that's the best - you can charge for rides - up and down!! Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Monday, December 02, 2013 4:20 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Grossing tables I'm currently designing a new GI lab. Any advice on grossing stations? The only name I know is Mopec. Is that the best one to go with or are there better ones out there? Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From doug.porter <@t> caplab.org Mon Dec 2 15:35:35 2013 From: doug.porter <@t> caplab.org (Douglas Porter) Date: Mon Dec 2 15:32:26 2013 Subject: [Histonet] RE: Grossing tables In-Reply-To: References: <47E9B2C01DDDD94881EACD2DC44EBC8802C035EA@D1PWPEXMBX05.mdanderson.edu> <5A33C952BB67F4468AF1F36D739212BCDD121F7B@JERRY.Gia.com> Message-ID: <00a101ceefa6$7068a980$5139fc80$@caplab.org> http://www.thermoscientific.com/en/products/pathology-work-stations.html Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.porter@caplab.org www.caplab.org ? ? The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, copying, forwarding or capture of this communication is strictly prohibited. If you have received this communication in error, please notify me immediately by return e-mail and delete this and all copies. Thank-you. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Monday, December 02, 2013 4:24 PM To: 'Amber McKenzie'; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Grossing tables I think that's the best - you can charge for rides - up and down!! Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Monday, December 02, 2013 4:20 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Grossing tables I'm currently designing a new GI lab. Any advice on grossing stations? The only name I know is Mopec. Is that the best one to go with or are there better ones out there? Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From fbozkurt <@t> gmail.com Mon Dec 2 15:37:21 2013 From: fbozkurt <@t> gmail.com (Mehmet Fatih BOZKURT) Date: Mon Dec 2 15:37:25 2013 Subject: [Histonet] Re: [IHCRG] macrophage eq markers? In-Reply-To: References: Message-ID: In which animals works also this antibody ? Thanks On Mon, Dec 2, 2013 at 11:20 PM, Jan Shivers wrote: > Dako's MAC 387 (cat. #: M0747) > > > On Mon, Dec 2, 2013 at 2:09 PM, Jackie Ferracone wrote: > >> Hi All, >> >> Looking for good macrophage markers for equine tissue, FFPE IHC .? >> >> Thanks >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> Jackie Ferracone, B.Sc. >> >> Research Specialist >> >> Dept. of Pathobiology >> >> University of Pennsylvania >> >> School of Veterinary Medicine >> >> 382 West Street Road >> >> Kennett Square, PA 19348-1692 >> >> 610-925-6391 >> >> 610-350-8860 (cell) >> >> 610-925-6810 (fax) >> >> >> >> -- >> -- >> You received this message because you are subscribed to the Google >> Groups "ihcrg" group. The IHC Resource Group is a standing committee >> within the National Society for Histotechnology. >> >> To post to this group, send email to ihcrg@googlegroups.com >> To unsubscribe from this group, send email to >> ihcrg+unsubscribe@googlegroups.com >> For more options, visit this group at >> http://groups.google.com/group/ihcrg?hl=en >> >> To contact the National Society for Histotechnology, email: histo@nsh.orgor call >> 443.535.4060. >> >> --- >> You received this message because you are subscribed to the Google Groups >> "ihcrg" group. >> To unsubscribe from this group and stop receiving emails from it, send an >> email to ihcrg+unsubscribe@googlegroups.com. >> For more options, visit https://groups.google.com/groups/opt_out. >> > > > > -- > Jan Shivers > Senior Scientist > IHC/Histology Section Head > Pathology Teaching Program > Veterinary Diagnostic Laboratory > University of Minnesota > 1333 Gortner Ave. > St. Paul, MN 55108 > 612-624-7297 > shive003@umn.edu > > -- > -- > You received this message because you are subscribed to the Google > Groups "ihcrg" group. The IHC Resource Group is a standing committee > within the National Society for Histotechnology. > > To post to this group, send email to ihcrg@googlegroups.com > To unsubscribe from this group, send email to > ihcrg+unsubscribe@googlegroups.com > For more options, visit this group at > http://groups.google.com/group/ihcrg?hl=en > > To contact the National Society for Histotechnology, email: histo@nsh.orgor call > 443.535.4060. > > --- > You received this message because you are subscribed to the Google Groups > "ihcrg" group. > To unsubscribe from this group and stop receiving emails from it, send an > email to ihcrg+unsubscribe@googlegroups.com. > For more options, visit https://groups.google.com/groups/opt_out. > -- Mehmet Fatih BOZKURT, DVM, PhD Afyon Kocatepe University Faculty of Veterinary Medicine Department of Pathology 03030, ANS Campus Afyonkarahisar-TURKEY Tel: +902722281312-16173/16237 From lpwenk <@t> sbcglobal.net Mon Dec 2 15:46:16 2013 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Mon Dec 2 15:46:21 2013 Subject: [Histonet] Chris van der Loos Message-ID: Sad news. I received an email over the weekend from a histotech, that Chris van der Loos died on Nov. 26, 2013. I was able to talk with the NSH office today, and got it confirmed. There is information about Chris on the NSH webpage as of later this morning. http://www.nsh.org/content/remembering-dr-chris-van-der-loos-august-2-1955-%E2%80%93-november-26-2013 Peggy A. Wenk. HTL(ASCP)SLS From shive003 <@t> umn.edu Mon Dec 2 15:59:11 2013 From: shive003 <@t> umn.edu (Jan Shivers) Date: Mon Dec 2 15:59:17 2013 Subject: [Histonet] Re: [IHCRG] macrophage eq markers? In-Reply-To: References: Message-ID: I've had it to work in cat, cow, dog, fox, horse, otter, pig, and raccoon in my lab. According to vendor - it's supposed to also work in guinea pig, monkey, rabbit, and rat, but I have not tried it in those animals Needs either enzyme digestion or heat antigen retrieval. Best regards, Jan Shivers On Mon, Dec 2, 2013 at 3:37 PM, Mehmet Fatih BOZKURT wrote: > In which animals works also this antibody ? > > Thanks > > > On Mon, Dec 2, 2013 at 11:20 PM, Jan Shivers wrote: > >> Dako's MAC 387 (cat. #: M0747) >> >> >> On Mon, Dec 2, 2013 at 2:09 PM, Jackie Ferracone wrote: >> >>> Hi All, >>> >>> Looking for good macrophage markers for equine tissue, FFPE IHC .? >>> >>> Thanks >>> >>> >>> >>> >>> >>> >>> >>> >>> >>> >>> >>> >>> >>> >>> >>> Jackie Ferracone, B.Sc. >>> >>> Research Specialist >>> >>> Dept. of Pathobiology >>> >>> University of Pennsylvania >>> >>> School of Veterinary Medicine >>> >>> 382 West Street Road >>> >>> Kennett Square, PA 19348-1692 >>> >>> 610-925-6391 >>> >>> 610-350-8860 (cell) >>> >>> 610-925-6810 (fax) >>> >>> >>> >>> -- >>> -- >>> You received this message because you are subscribed to the Google >>> Groups "ihcrg" group. The IHC Resource Group is a standing committee >>> within the National Society for Histotechnology. >>> >>> To post to this group, send email to ihcrg@googlegroups.com >>> To unsubscribe from this group, send email to >>> ihcrg+unsubscribe@googlegroups.com >>> For more options, visit this group at >>> http://groups.google.com/group/ihcrg?hl=en >>> >>> To contact the National Society for Histotechnology, email: >>> histo@nsh.org or call 443.535.4060. >>> >>> --- >>> You received this message because you are subscribed to the Google >>> Groups "ihcrg" group. >>> To unsubscribe from this group and stop receiving emails from it, send >>> an email to ihcrg+unsubscribe@googlegroups.com. >>> For more options, visit https://groups.google.com/groups/opt_out. >>> >> >> >> >> -- >> Jan Shivers >> Senior Scientist >> IHC/Histology Section Head >> Pathology Teaching Program >> Veterinary Diagnostic Laboratory >> University of Minnesota >> 1333 Gortner Ave. >> St. Paul, MN 55108 >> 612-624-7297 >> shive003@umn.edu >> >> -- >> -- >> You received this message because you are subscribed to the Google >> Groups "ihcrg" group. The IHC Resource Group is a standing committee >> within the National Society for Histotechnology. >> >> To post to this group, send email to ihcrg@googlegroups.com >> To unsubscribe from this group, send email to >> ihcrg+unsubscribe@googlegroups.com >> For more options, visit this group at >> http://groups.google.com/group/ihcrg?hl=en >> >> To contact the National Society for Histotechnology, email: histo@nsh.orgor call >> 443.535.4060. >> >> --- >> You received this message because you are subscribed to the Google Groups >> "ihcrg" group. >> To unsubscribe from this group and stop receiving emails from it, send an >> email to ihcrg+unsubscribe@googlegroups.com. >> For more options, visit https://groups.google.com/groups/opt_out. >> > > > > -- > Mehmet Fatih BOZKURT, DVM, PhD > Afyon Kocatepe University > Faculty of Veterinary Medicine > Department of Pathology > 03030, ANS Campus > Afyonkarahisar-TURKEY > Tel: +902722281312-16173/16237 > -- Jan Shivers Senior Scientist IHC/Histology Section Head Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive003@umn.edu From BDeBrosse-Serra <@t> isisph.com Mon Dec 2 16:25:43 2013 From: BDeBrosse-Serra <@t> isisph.com (Bea DeBrosse-Serra) Date: Mon Dec 2 16:25:49 2013 Subject: [Histonet] Chris van der Loos In-Reply-To: References: Message-ID: Very sad news indeed. He will be missed dearly! Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk Sent: Monday, December 02, 2013 1:46 PM To: Histonet Subject: [Histonet] Chris van der Loos Sad news. I received an email over the weekend from a histotech, that Chris van der Loos died on Nov. 26, 2013. I was able to talk with the NSH office today, and got it confirmed. There is information about Chris on the NSH webpage as of later this morning. http://www.nsh.org/content/remembering-dr-chris-van-der-loos-august-2-1955-%E2%80%93-november-26-2013 Peggy A. Wenk. HTL(ASCP)SLS _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tony.henwood <@t> health.nsw.gov.au Mon Dec 2 17:24:12 2013 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Mon Dec 2 17:24:25 2013 Subject: [Histonet] Chris van der Loos In-Reply-To: References: Message-ID: <6D6BD1DE8A5571489398B392A38A71579D476F5B@xmdb04.nch.kids> This is very sad. Chris was knowledgeable, with a wonderful sense of humour and always had time for his colleagues. He will be missed Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk Sent: Tuesday, 3 December 2013 8:46 AM To: Histonet Subject: [Histonet] Chris van der Loos Sad news. I received an email over the weekend from a histotech, that Chris van der Loos died on Nov. 26, 2013. I was able to talk with the NSH office today, and got it confirmed. There is information about Chris on the NSH webpage as of later this morning. http://www.nsh.org/content/remembering-dr-chris-van-der-loos-august-2-1955-%E2%80%93-november-26-2013 Peggy A. Wenk. HTL(ASCP)SLS _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From Jim.Manavis <@t> health.sa.gov.au Mon Dec 2 17:36:34 2013 From: Jim.Manavis <@t> health.sa.gov.au (Manavis, Jim (Health)) Date: Mon Dec 2 17:36:58 2013 Subject: [Histonet] Chris van der Loos Message-ID: <8FDCD9CF12830242808BE562200F67F97A0F7C9F81@EMSCM012.sagemsmrd01.sa.gov.au> I had the privilege and good fortune of getting to know and swap ideas with Chris after spending a few weeks with him in Breda back in 2001. Never short of advice and always willing to help and learn from others. To use an Australian expression "he was an absolute Cracker of a guy". Cheers Jim Jim Manavis Laboratory Manager Hanson Institute Centre for Neurological Diseases SA Pathology, Adelaide, SA, 5000 Australia Phone: 61-08-8222-3668 / 0401120697 FAX: 61-08-8222 3392 email: jim.manavis@imvs.sa.gov.au Disclaimer: Not this little black duck! From tony.henwood <@t> health.nsw.gov.au Mon Dec 2 17:55:59 2013 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Mon Dec 2 17:56:09 2013 Subject: [Histonet] RE: Chris van der Loos In-Reply-To: <8FDCD9CF12830242808BE562200F67F97A0F7C9F81@EMSCM012.sagemsmrd01.sa.gov.au> References: <8FDCD9CF12830242808BE562200F67F97A0F7C9F81@EMSCM012.sagemsmrd01.sa.gov.au> Message-ID: <6D6BD1DE8A5571489398B392A38A71579D476F8D@xmdb04.nch.kids> I agree Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Manavis, Jim (Health) Sent: Tuesday, 3 December 2013 10:37 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Chris van der Loos I had the privilege and good fortune of getting to know and swap ideas with Chris after spending a few weeks with him in Breda back in 2001. Never short of advice and always willing to help and learn from others. To use an Australian expression "he was an absolute Cracker of a guy". Cheers Jim Jim Manavis Laboratory Manager Hanson Institute Centre for Neurological Diseases SA Pathology, Adelaide, SA, 5000 Australia Phone: 61-08-8222-3668 / 0401120697 FAX: 61-08-8222 3392 email: jim.manavis@imvs.sa.gov.au Disclaimer: Not this little black duck! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From barryrittman <@t> gmail.com Mon Dec 2 18:27:00 2013 From: barryrittman <@t> gmail.com (Barry Rittman) Date: Mon Dec 2 18:27:04 2013 Subject: [Histonet] rat's hind paw skin In-Reply-To: <17C4C4E7-B1A8-4D51-95AC-218FE8737291@med.lu.se> References: <17C4C4E7-B1A8-4D51-95AC-218FE8737291@med.lu.se> Message-ID: Hi Leila As no one seems to have responded to your email I will add a few items. I used to do some work on wound healing on mouse footpads so circumstances are the same. If you are looking for melanin routine fixation and processing should be finem, melanin will remain. Only thing is that the epidermis is tough and there is not much dermis in this region. Need to make sure that you are careful in cutting sections or the epidermis will separate. Hope this helps if you need any more details please email me. Barry On Mon, Dec 2, 2013 at 3:44 AM, Leila Etemadi wrote: > Hello every body, > > First I would like to thank you all for your great feedbacks and attention > which is such a big improvement in my work, Thank you all! > > Then, I was wonder if any one has experience on this tissue: rat hind paw > skin ( which is hairless). > > Any inputs will be appreciated, whether is about any specific source where > I can find technical information about fixation procedure..., or about > sectioning,staining procedure? > > I am looking for melanin pigments and have no clue if it is possible to > see it there or not?!? > > Thanks in advance, > > Cheers > > Leila > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From amosbrooks <@t> gmail.com Mon Dec 2 18:37:47 2013 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Mon Dec 2 18:37:59 2013 Subject: [Histonet] (no subject) Message-ID: <2lh4bi16hrmsksyitrg8v4ik.1386031067734@email.android.com> Hi, There are a few ways of doing this. Perhaps the easiest is scraping the cells off while it is still in the media. Transfer it to a centrifuge tube and spin it down. Pour off the supernatant add formalism vortex it and centrifuge it again and pour off the supernatant again and add Histogel or agar. Then process as usual. This is about as close as you are going to get to treating it like normal tissue. Amos Message: 4 Date: Mon, 2 Dec 2013 17:06:24 +0000 From: Mike Tighe Subject: [Histonet] Embedding cells in Paraffin To: "histonet@lists.utsouthwestern.edu (histonet@lists.utsouthwestern.edu)" Message-ID: <46d5589df0864870848089e6780fb432@BY2PR07MB469.namprd07.prod.outlook.com> Content-Type: text/plain; charset="us-ascii" Has anyone tried to embed cells grown in tissue culture? I am trying to put some tissue culture cells through same stress as tissue would go through. Fixation, dehydration, and heat. Any ideas? I could re-suspend in OCT and then fix for extended time with NBF but that doesn't quite seem fair. Thanks for any ideas! From mturner <@t> CSILaboratories.com Tue Dec 3 07:21:58 2013 From: mturner <@t> CSILaboratories.com (Mark Turner) Date: Tue Dec 3 07:22:06 2013 Subject: [Histonet] Chris van der Loos In-Reply-To: References: Message-ID: <643626B74DE2814D8537057F40E1A10B046A8AB6@CSI-MX-NODEA.CSI-LABS.local> Chris will be missed dearly. Not only for his sense of humor and knowledge, but because the world is not the same today without him. He was a wonderful colleague. Thoughts and prayers are with his wife and children at this time. Mark Turner, Ph.D., HT(ASCP)QIHC CSI Laboratories Manager, Histology/IHC ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk Sent: Monday, December 02, 2013 4:46 PM To: Histonet Subject: [Histonet] Chris van der Loos Sad news. I received an email over the weekend from a histotech, that Chris van der Loos died on Nov. 26, 2013. I was able to talk with the NSH office today, and got it confirmed. There is information about Chris on the NSH webpage as of later this morning. http://www.nsh.org/content/remembering-dr-chris-van-der-loos-august-2-1955-%E2%80%93-november-26-2013 Peggy A. Wenk. HTL(ASCP)SLS _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mtighe <@t> trudeauinstitute.org Tue Dec 3 08:31:03 2013 From: mtighe <@t> trudeauinstitute.org (Mike Tighe) Date: Tue Dec 3 08:31:08 2013 Subject: [Histonet] Yahoo link Message-ID: <63f258ee13dc423ab0531cb811d5d71e@BY2PR07MB469.namprd07.prod.outlook.com> Anybody wonder who has the most harzardous job to your health? We're Number One!!! http://finance.yahoo.com/news/the-15-jobs-that-are-most-damaging-to-your-health-155706120.html Mike From BDeBrosse-Serra <@t> isisph.com Tue Dec 3 09:16:27 2013 From: BDeBrosse-Serra <@t> isisph.com (Bea DeBrosse-Serra) Date: Tue Dec 3 09:16:40 2013 Subject: [Histonet] RE: Yahoo link In-Reply-To: <63f258ee13dc423ab0531cb811d5d71e@BY2PR07MB469.namprd07.prod.outlook.com> References: <63f258ee13dc423ab0531cb811d5d71e@BY2PR07MB469.namprd07.prod.outlook.com> Message-ID: What an eye opener! Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Tighe Sent: Tuesday, December 03, 2013 6:31 AM To: histonet@lists.utsouthwestern.edu (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Yahoo link Anybody wonder who has the most harzardous job to your health? We're Number One!!! http://finance.yahoo.com/news/the-15-jobs-that-are-most-damaging-to-your-health-155706120.html Mike _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dmlaud <@t> laudierhistology.com Tue Dec 3 09:33:06 2013 From: dmlaud <@t> laudierhistology.com (Damien) Date: Tue Dec 3 09:33:12 2013 Subject: [Histonet] RE: Yahoo link In-Reply-To: References: <63f258ee13dc423ab0531cb811d5d71e@BY2PR07MB469.namprd07.prod.outlook.com> Message-ID: Wow,didn't expect that one! -Damien L. On Tue, Dec 3, 2013 at 10:16 AM, Bea DeBrosse-Serra < BDeBrosse-Serra@isisph.com> wrote: > What an eye opener! > > Beatrice DeBrosse-Serra HT(ASCP)QIHC > Isis Pharmaceuticals > Antisense Drug Discovery > 2855 Gazelle Ct. > Carlsbad, CA 92010 > 760-603-2371 > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Tighe > Sent: Tuesday, December 03, 2013 6:31 AM > To: histonet@lists.utsouthwestern.edu (histonet@lists.utsouthwestern.edu) > Subject: [Histonet] Yahoo link > > Anybody wonder who has the most harzardous job to your health? We're > Number One!!! > > > > > http://finance.yahoo.com/news/the-15-jobs-that-are-most-damaging-to-your-health-155706120.html > > > > Mike > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Damien Laudier Laudier Histology www.LaudierHistology.com From llang <@t> aipathology.com Tue Dec 3 09:42:11 2013 From: llang <@t> aipathology.com (LeAnn Lang) Date: Tue Dec 3 09:42:17 2013 Subject: [Histonet] Amended Reports Message-ID: <704247D5A09D004C9E6B115138D1703AB2BE31@hpserv001.aipathology.local> I need your help with the following situation. We receive specimens from an outside hospital and the specimen request slip and specimen container are given the accession number at that location. Once it is received in our laboratory, we process the specimen. A situation came up recently where the outside hospital mixed up the paperwork on three cases (so the wrong request slip was placed with the wrong specimen container, but it was not caught in our laboratory because they did not all come together). Anyhow, long story short, the original reports got reported as: The specimen request slip is labeled as "Joe Smith" and the specimen container is labeled as "Jane Doe". The specimen consists of........ After the reports were finalized, and sent back to the clinician, the mistake was identified. We made amended reports on our end to correct everything per CAP guidelines. The problem we are now running into is that the Medical Records dept is telling us that we are in HIPAA violation by having the two patient names on the report. I see their point, but don't know any other way of doing this. Anyone else ever deal with a situation like this? If so, how do you handle it? Thank you! LeAnn <>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<> LeAnn Lang Associates in Pathology Practice Administrator Phone: 715-847-0075 (ext 50259) llang@aipathology.com From AHutton <@t> dh.org Tue Dec 3 10:16:26 2013 From: AHutton <@t> dh.org (Hutton, Allison) Date: Tue Dec 3 10:16:34 2013 Subject: [Histonet] off site frozen sections Message-ID: <4ED8C96A8F20FC4F883A92E2A0A0D64AE69C@DH-MAIL01.dhorg.org> I am curious to find out if there is anybody out there that performs frozen sections off site (i.e hospital owned surgery center) and then performs the remaining pathology at the main hospital. We are currently looking into doing this and are stumbling over the logistics of how to make this happen. Any insight would be appreciated Thanks, Allison From Joyce.Weems <@t> emoryhealthcare.org Tue Dec 3 10:23:45 2013 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Tue Dec 3 10:24:04 2013 Subject: [Histonet] RE: off site frozen sections In-Reply-To: <4ED8C96A8F20FC4F883A92E2A0A0D64AE69C@DH-MAIL01.dhorg.org> References: <4ED8C96A8F20FC4F883A92E2A0A0D64AE69C@DH-MAIL01.dhorg.org> Message-ID: Our nearby surgery centers send the frozens to us. We have a distant hospital where the frozens are performed and the rest of the path is sent here, along with slides, etc. The pathologists rotate going to that hospital. Hope this helps, j Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hutton, Allison Sent: Tuesday, December 03, 2013 11:16 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] off site frozen sections I am curious to find out if there is anybody out there that performs frozen sections off site (i.e hospital owned surgery center) and then performs the remaining pathology at the main hospital. We are currently looking into doing this and are stumbling over the logistics of how to make this happen. Any insight would be appreciated Thanks, Allison _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From Timothy.Morken <@t> ucsfmedctr.org Tue Dec 3 10:24:33 2013 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Tue Dec 3 10:24:50 2013 Subject: [Histonet] RE: Yahoo link In-Reply-To: <63f258ee13dc423ab0531cb811d5d71e@BY2PR07MB469.namprd07.prod.outlook.com> References: <63f258ee13dc423ab0531cb811d5d71e@BY2PR07MB469.namprd07.prod.outlook.com> Message-ID: <761E2B5697F795489C8710BCC72141FF0B52C9@ex07.net.ucsf.edu> Great. Just what we need. Even so, we in the business can take these lists with a grain of K4[Fe(CN) 6] * 3H 2 O since we know that suitable precautions preclude most of the danger. For instance, I'll have to say that the histo lab here is wonderful in that it has such good ventilation (ie., extraction) that there is none of the usual chemical smell - no xylene, alcohol, specials chemicals that often assaults the senses in histology. Vendors that come here are amazed. Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Tighe Sent: Tuesday, December 03, 2013 6:31 AM To: histonet@lists.utsouthwestern.edu (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Yahoo link Anybody wonder who has the most harzardous job to your health? We're Number One!!! http://finance.yahoo.com/news/the-15-jobs-that-are-most-damaging-to-your-health-155706120.html Mike _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From doug.porter <@t> caplab.org Tue Dec 3 10:40:38 2013 From: doug.porter <@t> caplab.org (Douglas Porter) Date: Tue Dec 3 10:37:27 2013 Subject: [Histonet] RE: offsite frozen sections Message-ID: <002301cef046$6633a280$329ae780$@caplab.org> We have converted an ambulance to a mobile frozen section lab. We have a cryostat, microscope, eyewash station, etc., etc.. It, because it is a lab, is inspection by CAP on a regular two year rotation like our other labs. If you are interested, let me know and I'll send you some pictures to better demonstrate what we've done. We just drive to the location where the surgery is taking place and do the frozen section right there, on site. I say we, I mean our pathologist drives the mobile lab to the site, does the frozen section and returns with the specimen. They get their frozen section results by cell phone and we follow up with the hard copy report or fax copy. It's actually quite slick! Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.porter@caplab.org www.caplab.org ? ? The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, copying, forwarding or capture of this communication is strictly prohibited. If you have received this communication in error, please notify me immediately by return e-mail and delete this and all copies. Thank-you. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Tuesday, December 03, 2013 11:24 AM To: 'Hutton, Allison'; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: off site frozen sections Our nearby surgery centers send the frozens to us. We have a distant hospital where the frozens are performed and the rest of the path is sent here, along with slides, etc. The pathologists rotate going to that hospital. Hope this helps, j Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hutton, Allison Sent: Tuesday, December 03, 2013 11:16 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] off site frozen sections I am curious to find out if there is anybody out there that performs frozen sections off site (i.e hospital owned surgery center) and then performs the remaining pathology at the main hospital. We are currently looking into doing this and are stumbling over the logistics of how to make this happen. Any insight would be appreciated Thanks, Allison _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From contact <@t> excaliburpathology.com Tue Dec 3 10:40:21 2013 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Tue Dec 3 10:40:26 2013 Subject: [Histonet] RE: Yahoo link In-Reply-To: <761E2B5697F795489C8710BCC72141FF0B52C9@ex07.net.ucsf.edu> References: <63f258ee13dc423ab0531cb811d5d71e@BY2PR07MB469.namprd07.prod.outlook.com> <761E2B5697F795489C8710BCC72141FF0B52C9@ex07.net.ucsf.edu> Message-ID: <1386088821.21321.YahooMailNeo@web5704.biz.mail.ne1.yahoo.com> Just what I was thinking. We will never get enough new students now! With proper training and equipment, we do the job safely! And you can drown in an inch of water. Is that in the MSDS! ? Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 5830 N Blue Lake Dr. Please note new address! Norman, OK 73069 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ________________________________ From: "Morken, Timothy" To: 'Mike Tighe' ; "histonet@lists.utsouthwestern.edu (histonet@lists.utsouthwestern.edu)" Sent: Tuesday, December 3, 2013 10:24 AM Subject: [Histonet] RE: Yahoo link Great. Just what we need. Even so, we in the business can take these lists with a grain of K4[Fe(CN) 6] * 3H 2 O since we know that suitable precautions preclude most of the danger. For instance, I'll have to say that the histo lab here is wonderful in that it has such good ventilation (ie.,? extraction) that there is none of the usual chemical smell - no xylene, alcohol, specials chemicals that often assaults the senses in histology. Vendors that come here are amazed. Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Tighe Sent: Tuesday, December 03, 2013 6:31 AM To: histonet@lists.utsouthwestern.edu (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Yahoo link Anybody wonder who has the most harzardous job to your health? We're Number One!!! http://finance.yahoo.com/news/the-15-jobs-that-are-most-damaging-to-your-health-155706120.html Mike _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cls71877 <@t> gmail.com Tue Dec 3 10:40:37 2013 From: cls71877 <@t> gmail.com (Cristi Rigazio) Date: Tue Dec 3 10:41:41 2013 Subject: [Histonet] Yahoo link In-Reply-To: <63f258ee13dc423ab0531cb811d5d71e@BY2PR07MB469.namprd07.prod.outlook.com> References: <63f258ee13dc423ab0531cb811d5d71e@BY2PR07MB469.namprd07.prod.outlook.com> Message-ID: <95408155-9911-4742-A1F2-645FBC1A1A97@gmail.com> Thank you for sharing that! Yikes! Sent from my iPhone On Dec 3, 2013, at 6:31 AM, Mike Tighe wrote: > Anybody wonder who has the most harzardous job to your health? We're Number One!!! > > > > http://finance.yahoo.com/news/the-15-jobs-that-are-most-damaging-to-your-health-155706120.html > > > > Mike > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek <@t> digestivespecialists.com Tue Dec 3 11:00:34 2013 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Tue Dec 3 11:00:40 2013 Subject: [Histonet] Yahoo link In-Reply-To: <95408155-9911-4742-A1F2-645FBC1A1A97@gmail.com> References: <63f258ee13dc423ab0531cb811d5d71e@BY2PR07MB469.namprd07.prod.outlook.com> <95408155-9911-4742-A1F2-645FBC1A1A97@gmail.com> Message-ID: <5A2BD13465E061429D6455C8D6B40E391675D0BA08@IBMB7Exchange.digestivespecialists.com> Wow! And I've survived 40 years with only 2 "nicks" in this perilous field! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cristi Rigazio Sent: Tuesday, December 03, 2013 11:41 AM To: Mike Tighe Cc: histonet@lists.utsouthwestern.edu (histonet@lists.utsouthwestern.edu) Subject: Re: [Histonet] Yahoo link Thank you for sharing that! Yikes! Sent from my iPhone On Dec 3, 2013, at 6:31 AM, Mike Tighe wrote: > Anybody wonder who has the most harzardous job to your health? We're Number One!!! > > > > http://finance.yahoo.com/news/the-15-jobs-that-are-most-damaging-to-your-health-155706120.html > > > > Mike > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lcolbert <@t> pathmdlabs.com Tue Dec 3 11:09:06 2013 From: lcolbert <@t> pathmdlabs.com (Laurie Colbert) Date: Tue Dec 3 11:13:53 2013 Subject: [Histonet] RE: Amended Reports In-Reply-To: <704247D5A09D004C9E6B115138D1703AB2BE31@hpserv001.aipathology.local> References: <704247D5A09D004C9E6B115138D1703AB2BE31@hpserv001.aipathology.local> Message-ID: <12ECD7346266D74691EC2BFC75285E452F365BF3@BFL323E10.pathmdlabs.local> The specimen container and requisition should always be compared before accessioning. If the name on the specimen container label and the name on the requisition do not match, we do not accept the specimen. It should be sent back to be properly labeled. Laurie Colbert, HT (ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LeAnn Lang Sent: Tuesday, December 03, 2013 7:42 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Amended Reports I need your help with the following situation. We receive specimens from an outside hospital and the specimen request slip and specimen container are given the accession number at that location. Once it is received in our laboratory, we process the specimen. A situation came up recently where the outside hospital mixed up the paperwork on three cases (so the wrong request slip was placed with the wrong specimen container, but it was not caught in our laboratory because they did not all come together). Anyhow, long story short, the original reports got reported as: The specimen request slip is labeled as "Joe Smith" and the specimen container is labeled as "Jane Doe". The specimen consists of........ After the reports were finalized, and sent back to the clinician, the mistake was identified. We made amended reports on our end to correct everything per CAP guidelines. The problem we are now running into is that the Medical Records dept is telling us that we are in HIPAA violation by having the two patient names on the report. I see their point, but don't know any other way of doing this. Anyone else ever deal with a situation like this? If so, how do you handle it? Thank you! LeAnn <>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<> LeAnn Lang Associates in Pathology Practice Administrator Phone: 715-847-0075 (ext 50259) llang@aipathology.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From POWELL_SA <@t> mercer.edu Tue Dec 3 11:17:31 2013 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Tue Dec 3 11:17:35 2013 Subject: [Histonet] Yahoo link In-Reply-To: <1386088821.21321.YahooMailNeo@web5704.biz.mail.ne1.yahoo.com> References: <63f258ee13dc423ab0531cb811d5d71e@BY2PR07MB469.namprd07.prod.outlook.com> <761E2B5697F795489C8710BCC72141FF0B52C9@ex07.net.ucsf.edu> <1386088821.21321.YahooMailNeo@web5704.biz.mail.ne1.yahoo.com> Message-ID: <9BF995BC0E47744E9673A41486E24EE25845D838DD@MERCERMAIL.MercerU.local> When I entered the profession I was told the average life expectancy of a histotechs was 20 years from hiring. That scared me but I was already hooked. I have been doing this 51 years, so maybe good laboratory practices can help, in spite of bad ventilation and all those other dangers mentioned. Shirley Powell Antique Histotech -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Tuesday, December 03, 2013 11:40 AM To: Morken, Timothy; Histonet Subject: Re: [Histonet] RE: Yahoo link Just what I was thinking. We will never get enough new students now! With proper training and equipment, we do the job safely! And you can drown in an inch of water. Is that in the MSDS! ? Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 5830 N Blue Lake Dr. Please note new address! Norman, OK 73069 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ________________________________ From: "Morken, Timothy" To: 'Mike Tighe' ; "histonet@lists.utsouthwestern.edu (histonet@lists.utsouthwestern.edu)" Sent: Tuesday, December 3, 2013 10:24 AM Subject: [Histonet] RE: Yahoo link Great. Just what we need. Even so, we in the business can take these lists with a grain of K4[Fe(CN) 6] * 3H 2 O since we know that suitable precautions preclude most of the danger. For instance, I'll have to say that the histo lab here is wonderful in that it has such good ventilation (ie.,? extraction) that there is none of the usual chemical smell - no xylene, alcohol, specials chemicals that often assaults the senses in histology. Vendors that come here are amazed. Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Tighe Sent: Tuesday, December 03, 2013 6:31 AM To: histonet@lists.utsouthwestern.edu (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Yahoo link Anybody wonder who has the most harzardous job to your health? We're Number One!!! http://finance.yahoo.com/news/the-15-jobs-that-are-most-damaging-to-your-health-155706120.html Mike _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Tue Dec 3 11:22:08 2013 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Tue Dec 3 11:22:23 2013 Subject: [Histonet] RE: Yahoo link In-Reply-To: <9BF995BC0E47744E9673A41486E24EE25845D838DD@MERCERMAIL.MercerU.local> References: <63f258ee13dc423ab0531cb811d5d71e@BY2PR07MB469.namprd07.prod.outlook.com> <761E2B5697F795489C8710BCC72141FF0B52C9@ex07.net.ucsf.edu> <1386088821.21321.YahooMailNeo@web5704.biz.mail.ne1.yahoo.com> <9BF995BC0E47744E9673A41486E24EE25845D838DD@MERCERMAIL.MercerU.local> Message-ID: <761E2B5697F795489C8710BCC72141FF0B534C@ex07.net.ucsf.edu> Well, Shirley, you are actually an Angel, so nothing will ever stop you!! (from an old Georgia Society hand). Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shirley A. Powell Sent: Tuesday, December 03, 2013 9:18 AM To: Histonet Subject: [Histonet] Yahoo link When I entered the profession I was told the average life expectancy of a histotechs was 20 years from hiring. That scared me but I was already hooked. I have been doing this 51 years, so maybe good laboratory practices can help, in spite of bad ventilation and all those other dangers mentioned. Shirley Powell Antique Histotech -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Tuesday, December 03, 2013 11:40 AM To: Morken, Timothy; Histonet Subject: Re: [Histonet] RE: Yahoo link Just what I was thinking. We will never get enough new students now! With proper training and equipment, we do the job safely! And you can drown in an inch of water. Is that in the MSDS! ? Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 5830 N Blue Lake Dr. Please note new address! Norman, OK 73069 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ________________________________ From: "Morken, Timothy" To: 'Mike Tighe' ; "histonet@lists.utsouthwestern.edu (histonet@lists.utsouthwestern.edu)" Sent: Tuesday, December 3, 2013 10:24 AM Subject: [Histonet] RE: Yahoo link Great. Just what we need. Even so, we in the business can take these lists with a grain of K4[Fe(CN) 6] * 3H 2 O since we know that suitable precautions preclude most of the danger. For instance, I'll have to say that the histo lab here is wonderful in that it has such good ventilation (ie.,? extraction) that there is none of the usual chemical smell - no xylene, alcohol, specials chemicals that often assaults the senses in histology. Vendors that come here are amazed. Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Tighe Sent: Tuesday, December 03, 2013 6:31 AM To: histonet@lists.utsouthwestern.edu (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Yahoo link Anybody wonder who has the most harzardous job to your health? We're Number One!!! http://finance.yahoo.com/news/the-15-jobs-that-are-most-damaging-to-your-health-155706120.html Mike _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From billodonnell <@t> catholichealth.net Tue Dec 3 11:22:33 2013 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Tue Dec 3 11:22:37 2013 Subject: [Histonet] RE: Yahoo link In-Reply-To: <9BF995BC0E47744E9673A41486E24EE25845D838DD@MERCERMAIL.MercerU.local> References: <63f258ee13dc423ab0531cb811d5d71e@BY2PR07MB469.namprd07.prod.outlook.com> <761E2B5697F795489C8710BCC72141FF0B52C9@ex07.net.ucsf.edu> <1386088821.21321.YahooMailNeo@web5704.biz.mail.ne1.yahoo.com> <9BF995BC0E47744E9673A41486E24EE25845D838DD@MERCERMAIL.MercerU.local> Message-ID: We're #1! We're #1! We're #1! We're #1! We're #1! I didn't think anyone knew we existed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shirley A. Powell Sent: Tuesday, December 03, 2013 11:18 AM To: Histonet Subject: [Histonet] Yahoo link When I entered the profession I was told the average life expectancy of a histotechs was 20 years from hiring. That scared me but I was already hooked. I have been doing this 51 years, so maybe good laboratory practices can help, in spite of bad ventilation and all those other dangers mentioned. Shirley Powell Antique Histotech -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Tuesday, December 03, 2013 11:40 AM To: Morken, Timothy; Histonet Subject: Re: [Histonet] RE: Yahoo link Just what I was thinking. We will never get enough new students now! With proper training and equipment, we do the job safely! And you can drown in an inch of water. Is that in the MSDS! ? Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 5830 N Blue Lake Dr. Please note new address! Norman, OK 73069 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ________________________________ From: "Morken, Timothy" To: 'Mike Tighe' ; "histonet@lists.utsouthwestern.edu (histonet@lists.utsouthwestern.edu)" Sent: Tuesday, December 3, 2013 10:24 AM Subject: [Histonet] RE: Yahoo link Great. Just what we need. Even so, we in the business can take these lists with a grain of K4[Fe(CN) 6] * 3H 2 O since we know that suitable precautions preclude most of the danger. For instance, I'll have to say that the histo lab here is wonderful in that it has such good ventilation (ie.,? extraction) that there is none of the usual chemical smell - no xylene, alcohol, specials chemicals that often assaults the senses in histology. Vendors that come here are amazed. Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Tighe Sent: Tuesday, December 03, 2013 6:31 AM To: histonet@lists.utsouthwestern.edu (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Yahoo link Anybody wonder who has the most harzardous job to your health? We're Number One!!! http://finance.yahoo.com/news/the-15-jobs-that-are-most-damaging-to-your-health-155706120.html Mike _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. From Joyce.Weems <@t> emoryhealthcare.org Tue Dec 3 11:25:06 2013 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Tue Dec 3 11:25:13 2013 Subject: [Histonet] RE: Yahoo link In-Reply-To: <761E2B5697F795489C8710BCC72141FF0B534C@ex07.net.ucsf.edu> References: <63f258ee13dc423ab0531cb811d5d71e@BY2PR07MB469.namprd07.prod.outlook.com> <761E2B5697F795489C8710BCC72141FF0B52C9@ex07.net.ucsf.edu> <1386088821.21321.YahooMailNeo@web5704.biz.mail.ne1.yahoo.com> <9BF995BC0E47744E9673A41486E24EE25845D838DD@MERCERMAIL.MercerU.local> <761E2B5697F795489C8710BCC72141FF0B534C@ex07.net.ucsf.edu> Message-ID: Amen to that!! Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Tuesday, December 03, 2013 12:22 PM To: 'Shirley A. Powell'; Histonet Subject: [Histonet] RE: Yahoo link Well, Shirley, you are actually an Angel, so nothing will ever stop you!! (from an old Georgia Society hand). Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shirley A. Powell Sent: Tuesday, December 03, 2013 9:18 AM To: Histonet Subject: [Histonet] Yahoo link When I entered the profession I was told the average life expectancy of a histotechs was 20 years from hiring. That scared me but I was already hooked. I have been doing this 51 years, so maybe good laboratory practices can help, in spite of bad ventilation and all those other dangers mentioned. Shirley Powell Antique Histotech -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Tuesday, December 03, 2013 11:40 AM To: Morken, Timothy; Histonet Subject: Re: [Histonet] RE: Yahoo link Just what I was thinking. We will never get enough new students now! With proper training and equipment, we do the job safely! And you can drown in an inch of water. Is that in the MSDS! Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 5830 N Blue Lake Dr. Please note new address! Norman, OK 73069 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ________________________________ From: "Morken, Timothy" To: 'Mike Tighe' ; "histonet@lists.utsouthwestern.edu (histonet@lists.utsouthwestern.edu)" Sent: Tuesday, December 3, 2013 10:24 AM Subject: [Histonet] RE: Yahoo link Great. Just what we need. Even so, we in the business can take these lists with a grain of K4[Fe(CN) 6] * 3H 2 O since we know that suitable precautions preclude most of the danger. For instance, I'll have to say that the histo lab here is wonderful in that it has such good ventilation (ie., extraction) that there is none of the usual chemical smell - no xylene, alcohol, specials chemicals that often assaults the senses in histology. Vendors that come here are amazed. Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Tighe Sent: Tuesday, December 03, 2013 6:31 AM To: histonet@lists.utsouthwestern.edu (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Yahoo link Anybody wonder who has the most harzardous job to your health? We're Number One!!! http://finance.yahoo.com/news/the-15-jobs-that-are-most-damaging-to-your-health-155706120.html Mike _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From POWELL_SA <@t> mercer.edu Tue Dec 3 11:26:14 2013 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Tue Dec 3 11:26:19 2013 Subject: [Histonet] RE: Yahoo link In-Reply-To: <761E2B5697F795489C8710BCC72141FF0B534C@ex07.net.ucsf.edu> References: <63f258ee13dc423ab0531cb811d5d71e@BY2PR07MB469.namprd07.prod.outlook.com> <761E2B5697F795489C8710BCC72141FF0B52C9@ex07.net.ucsf.edu> <1386088821.21321.YahooMailNeo@web5704.biz.mail.ne1.yahoo.com> <9BF995BC0E47744E9673A41486E24EE25845D838DD@MERCERMAIL.MercerU.local> <761E2B5697F795489C8710BCC72141FF0B534C@ex07.net.ucsf.edu> Message-ID: <9BF995BC0E47744E9673A41486E24EE25845D838F7@MERCERMAIL.MercerU.local> Thanks Tim. You are sweet. People keep asking me when I was going to retire and I told them never, they will have to carry me out feet first. I think loving my job has kept me going this long. :) sp -----Original Message----- From: Morken, Timothy [mailto:Timothy.Morken@ucsfmedctr.org] Sent: Tuesday, December 03, 2013 12:22 PM To: Shirley A. Powell; Histonet Subject: RE: Yahoo link Well, Shirley, you are actually an Angel, so nothing will ever stop you!! (from an old Georgia Society hand). Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shirley A. Powell Sent: Tuesday, December 03, 2013 9:18 AM To: Histonet Subject: [Histonet] Yahoo link When I entered the profession I was told the average life expectancy of a histotechs was 20 years from hiring. That scared me but I was already hooked. I have been doing this 51 years, so maybe good laboratory practices can help, in spite of bad ventilation and all those other dangers mentioned. Shirley Powell Antique Histotech -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Tuesday, December 03, 2013 11:40 AM To: Morken, Timothy; Histonet Subject: Re: [Histonet] RE: Yahoo link Just what I was thinking. We will never get enough new students now! With proper training and equipment, we do the job safely! And you can drown in an inch of water. Is that in the MSDS! ? Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 5830 N Blue Lake Dr. Please note new address! Norman, OK 73069 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ________________________________ From: "Morken, Timothy" To: 'Mike Tighe' ; "histonet@lists.utsouthwestern.edu (histonet@lists.utsouthwestern.edu)" Sent: Tuesday, December 3, 2013 10:24 AM Subject: [Histonet] RE: Yahoo link Great. Just what we need. Even so, we in the business can take these lists with a grain of K4[Fe(CN) 6] * 3H 2 O since we know that suitable precautions preclude most of the danger. For instance, I'll have to say that the histo lab here is wonderful in that it has such good ventilation (ie.,? extraction) that there is none of the usual chemical smell - no xylene, alcohol, specials chemicals that often assaults the senses in histology. Vendors that come here are amazed. Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Tighe Sent: Tuesday, December 03, 2013 6:31 AM To: histonet@lists.utsouthwestern.edu (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Yahoo link Anybody wonder who has the most harzardous job to your health? We're Number One!!! http://finance.yahoo.com/news/the-15-jobs-that-are-most-damaging-to-your-health-155706120.html Mike _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 <@t> earthlink.net Tue Dec 3 11:48:08 2013 From: relia1 <@t> earthlink.net (Pam Barker) Date: Tue Dec 3 11:48:19 2013 Subject: [Histonet] RE: Yahoo link In-Reply-To: <9BF995BC0E47744E9673A41486E24EE25845D838F7@MERCERMAIL.MercerU.local> References: <63f258ee13dc423ab0531cb811d5d71e@BY2PR07MB469.namprd07.prod.outlook.com> <761E2B5697F795489C8710BCC72141FF0B52C9@ex07.net.ucsf.edu> <1386088821.21321.YahooMailNeo@web5704.biz.mail.ne1.yahoo.com> <9BF995BC0E47744E9673A41486E24EE25845D838DD@MERCERMAIL.MercerU.local> <761E2B5697F795489C8710BCC72141FF0B534C@ex07.net.ucsf.edu> <9BF995BC0E47744E9673A41486E24EE25845D838F7@MERCERMAIL.MercerU.local> Message-ID: <004c01cef04f$d634cbf0$829e63d0$@earthlink.net> Maybe it's all the chemicals that keep all of the lovely ladies of histology so well preserved :) Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker ? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shirley A. Powell Sent: Tuesday, December 03, 2013 12:26 PM To: Morken, Timothy Cc: Histonet Subject: [Histonet] RE: Yahoo link Thanks Tim. You are sweet. People keep asking me when I was going to retire and I told them never, they will have to carry me out feet first. I think loving my job has kept me going this long. :) sp -----Original Message----- From: Morken, Timothy [mailto:Timothy.Morken@ucsfmedctr.org] Sent: Tuesday, December 03, 2013 12:22 PM To: Shirley A. Powell; Histonet Subject: RE: Yahoo link Well, Shirley, you are actually an Angel, so nothing will ever stop you!! (from an old Georgia Society hand). Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shirley A. Powell Sent: Tuesday, December 03, 2013 9:18 AM To: Histonet Subject: [Histonet] Yahoo link When I entered the profession I was told the average life expectancy of a histotechs was 20 years from hiring. That scared me but I was already hooked. I have been doing this 51 years, so maybe good laboratory practices can help, in spite of bad ventilation and all those other dangers mentioned. Shirley Powell Antique Histotech -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Tuesday, December 03, 2013 11:40 AM To: Morken, Timothy; Histonet Subject: Re: [Histonet] RE: Yahoo link Just what I was thinking. We will never get enough new students now! With proper training and equipment, we do the job safely! And you can drown in an inch of water. Is that in the MSDS! ? Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 5830 N Blue Lake Dr. Please note new address! Norman, OK 73069 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ________________________________ From: "Morken, Timothy" To: 'Mike Tighe' ; "histonet@lists.utsouthwestern.edu (histonet@lists.utsouthwestern.edu)" Sent: Tuesday, December 3, 2013 10:24 AM Subject: [Histonet] RE: Yahoo link Great. Just what we need. Even so, we in the business can take these lists with a grain of K4[Fe(CN) 6] * 3H 2 O since we know that suitable precautions preclude most of the danger. For instance, I'll have to say that the histo lab here is wonderful in that it has such good ventilation (ie.,? extraction) that there is none of the usual chemical smell - no xylene, alcohol, specials chemicals that often assaults the senses in histology. Vendors that come here are amazed. Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Tighe Sent: Tuesday, December 03, 2013 6:31 AM To: histonet@lists.utsouthwestern.edu (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Yahoo link Anybody wonder who has the most harzardous job to your health? We're Number One!!! http://finance.yahoo.com/news/the-15-jobs-that-are-most-damaging-to-your-hea lth-155706120.html Mike _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BDeBrosse-Serra <@t> isisph.com Tue Dec 3 12:00:37 2013 From: BDeBrosse-Serra <@t> isisph.com (Bea DeBrosse-Serra) Date: Tue Dec 3 12:00:45 2013 Subject: [Histonet] RE: Yahoo link In-Reply-To: <004c01cef04f$d634cbf0$829e63d0$@earthlink.net> References: <63f258ee13dc423ab0531cb811d5d71e@BY2PR07MB469.namprd07.prod.outlook.com> <761E2B5697F795489C8710BCC72141FF0B52C9@ex07.net.ucsf.edu> <1386088821.21321.YahooMailNeo@web5704.biz.mail.ne1.yahoo.com> <9BF995BC0E47744E9673A41486E24EE25845D838DD@MERCERMAIL.MercerU.local> <761E2B5697F795489C8710BCC72141FF0B534C@ex07.net.ucsf.edu> <9BF995BC0E47744E9673A41486E24EE25845D838F7@MERCERMAIL.MercerU.local> <004c01cef04f$d634cbf0$829e63d0$@earthlink.net> Message-ID: Good one, Pam! :-) Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pam Barker Sent: Tuesday, December 03, 2013 9:48 AM To: 'Shirley A. Powell'; 'Morken, Timothy' Cc: 'Histonet' Subject: RE: [Histonet] RE: Yahoo link Maybe it's all the chemicals that keep all of the lovely ladies of histology so well preserved :) Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker ? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shirley A. Powell Sent: Tuesday, December 03, 2013 12:26 PM To: Morken, Timothy Cc: Histonet Subject: [Histonet] RE: Yahoo link Thanks Tim. You are sweet. People keep asking me when I was going to retire and I told them never, they will have to carry me out feet first. I think loving my job has kept me going this long. :) sp -----Original Message----- From: Morken, Timothy [mailto:Timothy.Morken@ucsfmedctr.org] Sent: Tuesday, December 03, 2013 12:22 PM To: Shirley A. Powell; Histonet Subject: RE: Yahoo link Well, Shirley, you are actually an Angel, so nothing will ever stop you!! (from an old Georgia Society hand). Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shirley A. Powell Sent: Tuesday, December 03, 2013 9:18 AM To: Histonet Subject: [Histonet] Yahoo link When I entered the profession I was told the average life expectancy of a histotechs was 20 years from hiring. That scared me but I was already hooked. I have been doing this 51 years, so maybe good laboratory practices can help, in spite of bad ventilation and all those other dangers mentioned. Shirley Powell Antique Histotech -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Tuesday, December 03, 2013 11:40 AM To: Morken, Timothy; Histonet Subject: Re: [Histonet] RE: Yahoo link Just what I was thinking. We will never get enough new students now! With proper training and equipment, we do the job safely! And you can drown in an inch of water. Is that in the MSDS! ? Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 5830 N Blue Lake Dr. Please note new address! Norman, OK 73069 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ________________________________ From: "Morken, Timothy" To: 'Mike Tighe' ; "histonet@lists.utsouthwestern.edu (histonet@lists.utsouthwestern.edu)" Sent: Tuesday, December 3, 2013 10:24 AM Subject: [Histonet] RE: Yahoo link Great. Just what we need. Even so, we in the business can take these lists with a grain of K4[Fe(CN) 6] * 3H 2 O since we know that suitable precautions preclude most of the danger. For instance, I'll have to say that the histo lab here is wonderful in that it has such good ventilation (ie.,? extraction) that there is none of the usual chemical smell - no xylene, alcohol, specials chemicals that often assaults the senses in histology. Vendors that come here are amazed. Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Tighe Sent: Tuesday, December 03, 2013 6:31 AM To: histonet@lists.utsouthwestern.edu (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Yahoo link Anybody wonder who has the most harzardous job to your health? We're Number One!!! http://finance.yahoo.com/news/the-15-jobs-that-are-most-damaging-to-your-hea lth-155706120.html Mike _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Donna.Millard <@t> prlnet.com Tue Dec 3 12:30:18 2013 From: Donna.Millard <@t> prlnet.com (Donna Millard) Date: Tue Dec 3 12:30:30 2013 Subject: [Histonet] off site frozen sections Message-ID: <64D11CD058311340A1966C234C6B0B891220D32E@PRLEXCH02.prlnet.com> We perform frozen sections in several off-site locations-hospitals and surgery centers. Pathologists travel to perform the service (we stopped sending techs with them-they prefer having a tech, but it took 2 people away when the pathologist is capable of performing the technical aspects, and are required for the diagnosis.). We have a medical director and CLIA license at the surgery centers since a diagnosis is occurring. Our pathologists serve as the medical directors of the hospital locations where we offer this service. In most facilities we assigned responsibility for the upkeep and maintenance to staff in that lab. At a couple (surgery centers where there isn't a lab) we routinely send a tech to do routine maintenance on the stain line and cryostat. If the pathologist is coming to our main lab location, they bring the specimen and slides with them. Otherwise they leave it for our courier to bring with their routine pick-ups. For close surgery centers, the pathologists call to see if the surgery is running as scheduled. For sites that are further away, unfortunately they sometimes have to wait because the surgery is not running on-time. Donna Millard Director of Anatomic Pathology Physicians Reference Laboratory, LLC 7800 W. 110th Street,Overland Park, KS 66210 Direct: 913-339-0485 Fax: 913-319-4156 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hutton, Allison Sent: Tuesday, December 03, 2013 11:16 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] off site frozen sections I am curious to find out if there is anybody out there that performs frozen sections off site (i.e hospital owned surgery center) and then performs the remaining pathology at the main hospital. We are currently looking into doing this and are stumbling over the logistics of how to make this happen. Any insight would be appreciated Thanks, Allison CONFIDENTIALITY NOTICE This message and any included attachments are from Physicians Reference Laboratory, LLC and are intended only for the addressee.The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call PRL's corporate offices in Overland Park, Kansas, U.S.A at (913)338-4070 From king.laurie <@t> marshfieldclinic.org Tue Dec 3 12:38:12 2013 From: king.laurie <@t> marshfieldclinic.org (King, Laurie J) Date: Tue Dec 3 12:38:19 2013 Subject: [Histonet] RE: off site frozen sections In-Reply-To: <4ED8C96A8F20FC4F883A92E2A0A0D64AE69C@DH-MAIL01.dhorg.org> References: <4ED8C96A8F20FC4F883A92E2A0A0D64AE69C@DH-MAIL01.dhorg.org> Message-ID: <7578207839F50248A7A6CD33517295EA4B8A4113@MCL-EXMB03.mfldclin.org> We do this using Remote Meeting Technologies. There is a camera mounted on the scope, the histotech grosses, stains, and drives the microsope, Pathology reads remotely and calls the surgeon. Then tissue is sent to the main campus for processing from one facility. The other facility is set up to process and send finished slides. Laurie -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hutton, Allison Sent: Tuesday, December 03, 2013 10:16 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] off site frozen sections I am curious to find out if there is anybody out there that performs frozen sections off site (i.e hospital owned surgery center) and then performs the remaining pathology at the main hospital. We are currently looking into doing this and are stumbling over the logistics of how to make this happen. Any insight would be appreciated Thanks, Allison _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ The contents of this message may contain private, protected and/or privileged information. If you received this message in error, you should destroy the e-mail message and any attachments or copies, and you are prohibited from retaining, distributing, disclosing or using any information contained within. Please contact the sender and advise of the erroneous delivery by return e-mail or telephone. Thank you for your cooperation. From joelleweaver <@t> hotmail.com Tue Dec 3 12:43:03 2013 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Tue Dec 3 12:43:07 2013 Subject: [Histonet] RE: Amended Reports In-Reply-To: <12ECD7346266D74691EC2BFC75285E452F365BF3@BFL323E10.pathmdlabs.local> References: <704247D5A09D004C9E6B115138D1703AB2BE31@hpserv001.aipathology.local>, <12ECD7346266D74691EC2BFC75285E452F365BF3@BFL323E10.pathmdlabs.local> Message-ID: This is just one method ( manual right now-no automation or barcoding- which I feel helps). But anyhow the SOP is that the name/specimen information has to be cross checked at each pre-analytic step ( transport manifest, accessioning, grossing, embedding, microtomy, stained slides for example). Each person has responsibility to stop/hold a specimen for an issue at any task. Identification or label discrepancies noticed at accessioning or grossing, are called to the submitting facility/clinician who is responsible to send someone when possible to re-label/identify. They sign a waiver/re-label form taking responsibility for the accuracy of the relabeled information.Do not normally send the specimen back, or normally reject due to the irreplaceable nature of many specimens, but if anything is amiss, this is documented at each check point. A copy of the re-label form is retained with the specimen requisition that travels to the Pathologist. The final call to reject or send back any really mis-identified specimen is at the discretion of the pathologist. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: lcolbert@pathmdlabs.com > To: llang@aipathology.com; histonet@lists.utsouthwestern.edu > Date: Tue, 3 Dec 2013 17:09:06 +0000 > CC: > Subject: [Histonet] RE: Amended Reports > > The specimen container and requisition should always be compared before accessioning. If the name on the specimen container label and the name on the requisition do not match, we do not accept the specimen. It should be sent back to be properly labeled. > > Laurie Colbert, HT (ASCP) > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LeAnn Lang > Sent: Tuesday, December 03, 2013 7:42 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Amended Reports > > I need your help with the following situation. > > > > We receive specimens from an outside hospital and the specimen request slip and specimen container are given the accession number at that location. Once it is received in our laboratory, we process the specimen. A situation came up recently where the outside hospital mixed up the paperwork on three cases (so the wrong request slip was placed with the wrong specimen container, but it was not caught in our laboratory because they did not all come together). Anyhow, long story short, the original reports got reported as: > > > > The specimen request slip is labeled as "Joe Smith" and the specimen container is labeled as "Jane Doe". The specimen consists of........ > > > > > > After the reports were finalized, and sent back to the clinician, the mistake was identified. We made amended reports on our end to correct everything per CAP guidelines. The problem we are now running into is that the Medical Records dept is telling us that we are in HIPAA violation by having the two patient names on the report. I see their point, but don't know any other way of doing this. > > > > Anyone else ever deal with a situation like this? If so, how do you handle it? > > > > Thank you! > > LeAnn > > > > <>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<> > > LeAnn Lang > > Associates in Pathology > > Practice Administrator > > Phone: 715-847-0075 (ext 50259) > > llang@aipathology.com > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Sherrian.McAnn <@t> va.gov Tue Dec 3 12:44:56 2013 From: Sherrian.McAnn <@t> va.gov (McAnn, Sherrian) Date: Tue Dec 3 12:45:02 2013 Subject: [Histonet] Nails falling off slides In-Reply-To: <1385128103.33810.YahooMailBasic@web161602.mail.bf1.yahoo.com> References: <1385128103.33810.YahooMailBasic@web161602.mail.bf1.yahoo.com> Message-ID: <61E2B58CECEF384094A363989D47C0900A70BE8F@VHAV17MSGA2.v17.med.va.gov> What works for me is this.First I face the block (we don't use any prepared softeners just regular processing) and then put the block in ammonia water ( 1%) in a small container on my coldplate and let it soak for a few minutes. I usually try not to go over 5 minutes but have gone over that once or twice without problems. I then rinse about 10- 15 seconds. I place the block back on my cold plate...allow it to get really cold. I cut at 2.5 - 3 microns. USE POSITIVELY CHARGED SLIDES. Once you cut the slides...(I usually cut about 3-6 slides so that I can choose the best ones) put them in an oven for some time to DRY THOROUGHLY. We usually let them dry overnight for the best results . We pick the best ones the next day for H&E and PASF. Now... sometimes we still have them wash off, but most of the time all or part stays on at least a few slides. I have been in this business for at least 25 years and this is an old method that we used "back in the day". It isn't perfect but does seem to get pretty good results. I wouldn't use less than pos. charged slides though. Hope that helps you....P.S. We have to let the pathologists know that they have to be patient when it comes to nails or else they will get washed off slides. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jill Cox Sent: Friday, November 22, 2013 7:48 AM To: Histonet@Lists. Edu Subject: [Histonet] Nails falling off slides Hi All, I know this has been mentioned a few times but need some help with nails falling off charged slides for H&E and PAS stains. I use Statlab nail prep softener and also use the good charged slides. I heat them 2 times for 25 minutes each. Is there an adhesive I can put on slide? Albumin? Any help would be much appreciated, thanks in advance.. Jill Cox HT, (ASCP) Histology Manager Arrowhead Dermatology 7747 W Deer Valley Rd Ste 250 Peoria AZ 85382 480-257-7222 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Sherrian.McAnn <@t> va.gov Tue Dec 3 13:01:46 2013 From: Sherrian.McAnn <@t> va.gov (McAnn, Sherrian) Date: Tue Dec 3 13:03:11 2013 Subject: [Histonet] Issue with Henry's Wonder Wax Enhanced Message-ID: <61E2B58CECEF384094A363989D47C0900A70BEA2@VHAV17MSGA2.v17.med.va.gov> Sounds like it may be static...try giving the blocks a little water . Put a little water in a small container on your cold plate and either dip a gauze into the water and pat the block just before cutting ( while in the chuck on the microtome) or place the block directly into the cold water for a few sec./min. before putting on the chuck to cut. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sandra Cheasty Sent: Monday, November 25, 2013 9:59 AM To: histonet@lists.utsouthwestern.edu Cc: lcalvo@svm.vetmed.wisc.edu; langeteig@svm.vetmed.wisc.edu; Barbara J Reese Subject: [Histonet] Issue with Henry's Wonder Wax Enhanced Hello fellow Techs, Has anyone noticed a change in the Henry's Wonder Wax, Enhanced? (Used for embedding, not infiltrating.) We have noticed that it is a lot "stickier" when we are sectioning. (To our picks, forceps, and fingers.) We have checked our temperatures, and all is well. I have asked the company, but they have not replied. Can anyone recommend another embedding paraffin? (We switched from Paraplast Plus about a year ago when we noticed changes after the original manufacturer was bought out.) Thank you, and have a safe food and family filled Thanksgiving! Sandy Sandra Cheasty Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Robert-Eytalis <@t> RiversideHealthCare.net Tue Dec 3 14:12:51 2013 From: Robert-Eytalis <@t> RiversideHealthCare.net (Eytalis, Robert A) Date: Tue Dec 3 14:13:06 2013 Subject: [Histonet] RE: offsite frozen sections In-Reply-To: <002301cef046$6633a280$329ae780$@caplab.org> References: <002301cef046$6633a280$329ae780$@caplab.org> Message-ID: I would like to see the photos. Robert A. Eytalis Laboratory Manager robert-eytalis@riversidehealthcare.net Phone: (815) 935-7256 ext. 5186 (815) 935-7535 Fax (815) 935-7068 Riverside Medical Center 350 N. Wall Street - Kankakee, IL 60901 http://mail.riversidehealthcare.net/owa/redir.aspx?C=qIzWpCGMNkq2SuiIt1v_2X7GmC1aOtAI7VlOiEQ974hhCQzwyDH6yJklmjoBIK92OWDFwivhIZs.&URL=http%3a%2f%2fwww.riversidemc.net%2f | http://mail.riversidehealthcare.net/owa/redir.aspx?C=qIzWpCGMNkq2SuiIt1v_2X7GmC1aOtAI7VlOiEQ974hhCQzwyDH6yJklmjoBIK92OWDFwivhIZs.&URL=http%3a%2f%2fwww.facebook.com%2fRiversideMC ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Douglas Porter [doug.porter@caplab.org] Sent: Tuesday, December 03, 2013 10:40 AM To: 'Weems, Joyce K.'; 'Hutton, Allison'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: offsite frozen sections We have converted an ambulance to a mobile frozen section lab. We have a cryostat, microscope, eyewash station, etc., etc.. It, because it is a lab, is inspection by CAP on a regular two year rotation like our other labs. If you are interested, let me know and I'll send you some pictures to better demonstrate what we've done. We just drive to the location where the surgery is taking place and do the frozen section right there, on site. I say we, I mean our pathologist drives the mobile lab to the site, does the frozen section and returns with the specimen. They get their frozen section results by cell phone and we follow up with the hard copy report or fax copy. It's actually quite slick! Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.porter@caplab.org www.caplab.org The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, copying, forwarding or capture of this communication is strictly prohibited. If you have received this communication in error, please notify me immediately by return e-mail and delete this and all copies. Thank-you. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Tuesday, December 03, 2013 11:24 AM To: 'Hutton, Allison'; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: off site frozen sections Our nearby surgery centers send the frozens to us. We have a distant hospital where the frozens are performed and the rest of the path is sent here, along with slides, etc. The pathologists rotate going to that hospital. Hope this helps, j Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hutton, Allison Sent: Tuesday, December 03, 2013 11:16 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] off site frozen sections I am curious to find out if there is anybody out there that performs frozen sections off site (i.e hospital owned surgery center) and then performs the remaining pathology at the main hospital. We are currently looking into doing this and are stumbling over the logistics of how to make this happen. Any insight would be appreciated Thanks, Allison _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carl.hobbs <@t> kcl.ac.uk Tue Dec 3 14:14:02 2013 From: carl.hobbs <@t> kcl.ac.uk (Hobbs, Carl) Date: Tue Dec 3 14:14:09 2013 Subject: [Histonet] rat's hind paw skin Message-ID: <443be70bbabb4024bc300c3774388bed@DB3PR03MB250.eurprd03.prod.outlook.com> Barry has "jolted" me into noticing...thanks. I occasionally prepare such specimens for Pwax for IHC to detect PGP9.5-positive nerve fibres in the epidermis. So....std 10% Formalin fixation ( us "PFA" if you must : I fix for 24 hrs minimum....no problem if longer fixed ) and processing. I get the researcher to place the fresh dissected footpad sample onto filter paper, so it fixes flat ( otherwise it might curl). Doesn't matter if it comes off after fixation.....it will stay flat during processing. Sure, if you are concerned that it will not stay flat, use plastic mesh inserts to "sandwich" the skin...place them in std plastic processing cassettes. If you are looking for melanin, you will see it on a H&E, if it is present. Appreciate any comments on this, Barry. Always grateful for further insights. Carl From trathborne <@t> somerset-healthcare.com Tue Dec 3 14:22:03 2013 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Tue Dec 3 14:22:30 2013 Subject: [Histonet] RE: Amended Reports In-Reply-To: References: <704247D5A09D004C9E6B115138D1703AB2BE31@hpserv001.aipathology.local>, <12ECD7346266D74691EC2BFC75285E452F365BF3@BFL323E10.pathmdlabs.local> Message-ID: <3AD061FE740D464FAC7BF6B5CFB75707A95A68FA@SMCMAIL01.somerset-healthcare.com> I really like the idea of a waiver/re-label form. Other than approval from the Laboratory Director, did you have to get approval from any other departments to put this form into use? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelle weaver Sent: Tuesday, December 03, 2013 1:43 PM To: Laurie Colbert; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Amended Reports This is just one method ( manual right now-no automation or barcoding- which I feel helps). But anyhow the SOP is that the name/specimen information has to be cross checked at each pre-analytic step ( transport manifest, accessioning, grossing, embedding, microtomy, stained slides for example). Each person has responsibility to stop/hold a specimen for an issue at any task. Identification or label discrepancies noticed at accessioning or grossing, are called to the submitting facility/clinician who is responsible to send someone when possible to re-label/identify. They sign a waiver/re-label form taking responsibility for the accuracy of the relabeled information.Do not normally send the specimen back, or normally reject due to the irreplaceable nature of many specimens, but if anything is amiss, this is documented at each check point. A copy of the re-label form is retained with the specimen requisition that travels to the Pathologist. The final call to reject or send back any really mis-identified specimen is at the discretion of the pathologist. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: lcolbert@pathmdlabs.com > To: llang@aipathology.com; histonet@lists.utsouthwestern.edu > Date: Tue, 3 Dec 2013 17:09:06 +0000 > CC: > Subject: [Histonet] RE: Amended Reports > > The specimen container and requisition should always be compared before accessioning. If the name on the specimen container label and the name on the requisition do not match, we do not accept the specimen. It should be sent back to be properly labeled. > > Laurie Colbert, HT (ASCP) > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LeAnn Lang > Sent: Tuesday, December 03, 2013 7:42 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Amended Reports > > I need your help with the following situation. > > > > We receive specimens from an outside hospital and the specimen request slip and specimen container are given the accession number at that location. Once it is received in our laboratory, we process the specimen. A situation came up recently where the outside hospital mixed up the paperwork on three cases (so the wrong request slip was placed with the wrong specimen container, but it was not caught in our laboratory because they did not all come together). Anyhow, long story short, the original reports got reported as: > > > > The specimen request slip is labeled as "Joe Smith" and the specimen container is labeled as "Jane Doe". The specimen consists of........ > > > > > > After the reports were finalized, and sent back to the clinician, the mistake was identified. We made amended reports on our end to correct everything per CAP guidelines. The problem we are now running into is that the Medical Records dept is telling us that we are in HIPAA violation by having the two patient names on the report. I see their point, but don't know any other way of doing this. > > > > Anyone else ever deal with a situation like this? If so, how do you handle it? > > > > Thank you! > > LeAnn > > > > <>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<> > > LeAnn Lang > > Associates in Pathology > > Practice Administrator > > Phone: 715-847-0075 (ext 50259) > > llang@aipathology.com > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brett_connolly <@t> merck.com Tue Dec 3 14:38:22 2013 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Tue Dec 3 14:38:54 2013 Subject: [Histonet] Stainer/coverslipper question Message-ID: Hello all, We are looking at options for automated stainer/glass coverslipper workstations. I browsed the Histonet archives and see many varied love/hate responses from over the years about reliability, need to babysit, bubbles etc. and I am wondering if things have improved as many past inquiries were fairly old. >From the archives, it seems that Lieca ST5020/CV5030 and the Sakura (Tissue-Tek) Prisma/Glas g2 are the main players. Our H&E workload is highly varied, but we want to also use the workstation for dehydrating and coverslipping our immuno slides that come off our IHC stainer in H2O. I would appreciate your recommendations/experiences. Thanks much, Brett Brett M. Connolly, Ph.D. Principal Scientist, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From joelleweaver <@t> hotmail.com Tue Dec 3 14:57:39 2013 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Tue Dec 3 14:57:45 2013 Subject: [Histonet] RE: Amended Reports In-Reply-To: <3AD061FE740D464FAC7BF6B5CFB75707A95A68FA@SMCMAIL01.somerset-healthcare.com> References: <704247D5A09D004C9E6B115138D1703AB2BE31@hpserv001.aipathology.local>, , <12ECD7346266D74691EC2BFC75285E452F365BF3@BFL323E10.pathmdlabs.local>, , <3AD061FE740D464FAC7BF6B5CFB75707A95A68FA@SMCMAIL01.somerset-healthcare.com> Message-ID: I developed mine here and I had the medical director approve. I would expect that it follows the same process for approval ( manager, lab director, medical director) as other SOP's for document control as other policies, procedures in most organizations? But may be outlined in the document control policy. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: trathborne@somerset-healthcare.com > To: joelleweaver@hotmail.com; lcolbert@pathmdlabs.com; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] RE: Amended Reports > Date: Tue, 3 Dec 2013 20:22:03 +0000 > > I really like the idea of a waiver/re-label form. Other than approval from the Laboratory Director, did you have to get approval from any other departments to put this form into use? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelle weaver > Sent: Tuesday, December 03, 2013 1:43 PM > To: Laurie Colbert; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] RE: Amended Reports > > This is just one method ( manual right now-no automation or barcoding- which I feel helps). > But anyhow the SOP is that the name/specimen information has to be cross checked at each pre-analytic step ( transport manifest, accessioning, grossing, embedding, microtomy, stained slides for example). > Each person has responsibility to stop/hold a specimen for an issue at any task. Identification or label discrepancies noticed at accessioning or grossing, are called to the submitting facility/clinician who is responsible to send someone when possible to re-label/identify. They sign a waiver/re-label form taking responsibility for the accuracy of the relabeled information.Do not normally send the specimen back, or normally reject due to the irreplaceable nature of many specimens, but if anything is amiss, this is documented at each check point. A copy of the re-label form is retained with the specimen requisition that travels to the Pathologist. The final call to reject or send back any really mis-identified specimen is at the discretion of the pathologist. > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > From: lcolbert@pathmdlabs.com > > To: llang@aipathology.com; histonet@lists.utsouthwestern.edu > > Date: Tue, 3 Dec 2013 17:09:06 +0000 > > CC: > > Subject: [Histonet] RE: Amended Reports > > > > The specimen container and requisition should always be compared before accessioning. If the name on the specimen container label and the name on the requisition do not match, we do not accept the specimen. It should be sent back to be properly labeled. > > > > Laurie Colbert, HT (ASCP) > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LeAnn Lang > > Sent: Tuesday, December 03, 2013 7:42 AM > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] Amended Reports > > > > I need your help with the following situation. > > > > > > > > We receive specimens from an outside hospital and the specimen request slip and specimen container are given the accession number at that location. Once it is received in our laboratory, we process the specimen. A situation came up recently where the outside hospital mixed up the paperwork on three cases (so the wrong request slip was placed with the wrong specimen container, but it was not caught in our laboratory because they did not all come together). Anyhow, long story short, the original reports got reported as: > > > > > > > > The specimen request slip is labeled as "Joe Smith" and the specimen container is labeled as "Jane Doe". The specimen consists of........ > > > > > > > > > > > > After the reports were finalized, and sent back to the clinician, the mistake was identified. We made amended reports on our end to correct everything per CAP guidelines. The problem we are now running into is that the Medical Records dept is telling us that we are in HIPAA violation by having the two patient names on the report. I see their point, but don't know any other way of doing this. > > > > > > > > Anyone else ever deal with a situation like this? If so, how do you handle it? > > > > > > > > Thank you! > > > > LeAnn > > > > > > > > <>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<> > > > > LeAnn Lang > > > > Associates in Pathology > > > > Practice Administrator > > > > Phone: 715-847-0075 (ext 50259) > > > > llang@aipathology.com > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From lcolbert <@t> pathmdlabs.com Tue Dec 3 15:09:34 2013 From: lcolbert <@t> pathmdlabs.com (Laurie Colbert) Date: Tue Dec 3 15:14:22 2013 Subject: [Histonet] RE: Stainer/coverslipper question In-Reply-To: References: Message-ID: <12ECD7346266D74691EC2BFC75285E452F365D17@BFL323E10.pathmdlabs.local> Hi Brett, I have used the Prisma Stainer with the film coverslipper connected, and loved them both. The Prisma stainer can hold 3 racks of 20 slides, so it is very nice for high volume. Our current stainer only holds one rack of 20, and slides can get backed up. The coverslipper is extremely fast and very trouble-free. I have heard that Sakura's glass coverslipper is good. We currently use the ClearVue glass coverslipper from Thermo, and it works well. Laurie Colbert, HT (ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Connolly, Brett M Sent: Tuesday, December 03, 2013 12:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Stainer/coverslipper question Hello all, We are looking at options for automated stainer/glass coverslipper workstations. I browsed the Histonet archives and see many varied love/hate responses from over the years about reliability, need to babysit, bubbles etc. and I am wondering if things have improved as many past inquiries were fairly old. >From the archives, it seems that Lieca ST5020/CV5030 and the Sakura (Tissue-Tek) Prisma/Glas g2 are the main players. Our H&E workload is highly varied, but we want to also use the workstation for dehydrating and coverslipping our immuno slides that come off our IHC stainer in H2O. I would appreciate your recommendations/experiences. Thanks much, Brett Brett M. Connolly, Ph.D. Principal Scientist, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lharris <@t> samhealth.org Tue Dec 3 15:33:28 2013 From: lharris <@t> samhealth.org (Lori Harris) Date: Tue Dec 3 15:33:39 2013 Subject: [Histonet] RE: Stainer/coverslipper question In-Reply-To: References: Message-ID: <450EDC37E404D142AF67D7314C954C8A3C8EF4711C@SHSMAILVI01.int.samhealth.net> Brett, We just received our new Symphony from Ventana and we love it!! Lori A. Harris, HT (ASCP) 541-768-6078 Histology Section Lead GSRMC Pathology Lab 3600 NW Samaritan Drive Corvallis, OR 97330 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Connolly, Brett M Sent: Tuesday, December 03, 2013 12:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Stainer/coverslipper question Hello all, We are looking at options for automated stainer/glass coverslipper workstations. I browsed the Histonet archives and see many varied love/hate responses from over the years about reliability, need to babysit, bubbles etc. and I am wondering if things have improved as many past inquiries were fairly old. >From the archives, it seems that Lieca ST5020/CV5030 and the Sakura (Tissue-Tek) Prisma/Glas g2 are the main players. Our H&E workload is highly varied, but we want to also use the workstation for dehydrating and coverslipping our immuno slides that come off our IHC stainer in H2O. I would appreciate your recommendations/experiences. Thanks much, Brett Brett M. Connolly, Ph.D. Principal Scientist, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From CIngles <@t> uwhealth.org Tue Dec 3 18:05:30 2013 From: CIngles <@t> uwhealth.org (Ingles Claire) Date: Tue Dec 3 18:05:36 2013 Subject: [Histonet] RE: Yahoo link In-Reply-To: <761E2B5697F795489C8710BCC72141FF0B534C@ex07.net.ucsf.edu> References: <63f258ee13dc423ab0531cb811d5d71e@BY2PR07MB469.namprd07.prod.outlook.com> <761E2B5697F795489C8710BCC72141FF0B52C9@ex07.net.ucsf.edu> <1386088821.21321.YahooMailNeo@web5704.biz.mail.ne1.yahoo.com> <9BF995BC0E47744E9673A41486E24EE25845D838DD@MERCERMAIL.MercerU.local>, <761E2B5697F795489C8710BCC72141FF0B534C@ex07.net.ucsf.edu> Message-ID: Old Histologists never die, they're just well fixed... Claire ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Morken, Timothy [Timothy.Morken@ucsfmedctr.org] Sent: Tuesday, December 03, 2013 11:22 AM To: 'Shirley A. Powell'; Histonet Subject: [Histonet] RE: Yahoo link Well, Shirley, you are actually an Angel, so nothing will ever stop you!! (from an old Georgia Society hand). Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shirley A. Powell Sent: Tuesday, December 03, 2013 9:18 AM To: Histonet Subject: [Histonet] Yahoo link When I entered the profession I was told the average life expectancy of a histotechs was 20 years from hiring. That scared me but I was already hooked. I have been doing this 51 years, so maybe good laboratory practices can help, in spite of bad ventilation and all those other dangers mentioned. Shirley Powell Antique Histotech -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Tuesday, December 03, 2013 11:40 AM To: Morken, Timothy; Histonet Subject: Re: [Histonet] RE: Yahoo link Just what I was thinking. We will never get enough new students now! With proper training and equipment, we do the job safely! And you can drown in an inch of water. Is that in the MSDS! Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 5830 N Blue Lake Dr. Please note new address! Norman, OK 73069 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ________________________________ From: "Morken, Timothy" To: 'Mike Tighe' ; "histonet@lists.utsouthwestern.edu (histonet@lists.utsouthwestern.edu)" Sent: Tuesday, December 3, 2013 10:24 AM Subject: [Histonet] RE: Yahoo link Great. Just what we need. Even so, we in the business can take these lists with a grain of K4[Fe(CN) 6] * 3H 2 O since we know that suitable precautions preclude most of the danger. For instance, I'll have to say that the histo lab here is wonderful in that it has such good ventilation (ie., extraction) that there is none of the usual chemical smell - no xylene, alcohol, specials chemicals that often assaults the senses in histology. Vendors that come here are amazed. Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Tighe Sent: Tuesday, December 03, 2013 6:31 AM To: histonet@lists.utsouthwestern.edu (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Yahoo link Anybody wonder who has the most harzardous job to your health? We're Number One!!! http://finance.yahoo.com/news/the-15-jobs-that-are-most-damaging-to-your-health-155706120.html Mike _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mikael.niku <@t> helsinki.fi Wed Dec 4 06:31:51 2013 From: mikael.niku <@t> helsinki.fi (Mikael Niku) Date: Wed Dec 4 06:32:07 2013 Subject: [Histonet] Leica MC120HD / MC170HD microscope cameras In-Reply-To: <5A33C952BB67F4468AF1F36D739212BCDD11E95A@JERRY.Gia.com> References: <5A33C952BB67F4468AF1F36D739212BCBD6EA704@JERRY.Gia.com> <95F6E043-FD0B-46B1-9A77-A20B7A72F1EF@yahoo.com> <5bae6ce3beab4ae38e21e5622fae4400@BLUPR07MB178.namprd07.prod.outlook.com> <5A33C952BB67F4468AF1F36D739212BCDD11E95A@JERRY.Gia.com> Message-ID: <529F20B7.5020708@helsinki.fi> Hello! I'm trying to find a new microscope camera for our histology & histopathology teaching lab. I would like to achieve full HD live image with good color reproduction. Anyone have experience on the Leica MC120/MC170 HD cameras? They are quite nice otherwise, but based on a quick demo, I'm not 100% convinced with the color quality. Recommendations for other cameras are also appreciated. With best regards, Mikael Niku, PhD University of Helsinki, Finland From Elizabeth.Cameron <@t> jax.org Wed Dec 4 07:24:15 2013 From: Elizabeth.Cameron <@t> jax.org (Elizabeth Cameron) Date: Wed Dec 4 07:24:26 2013 Subject: [Histonet] RE: Yahoo link In-Reply-To: References: <63f258ee13dc423ab0531cb811d5d71e@BY2PR07MB469.namprd07.prod.outlook.com> <761E2B5697F795489C8710BCC72141FF0B52C9@ex07.net.ucsf.edu> <1386088821.21321.YahooMailNeo@web5704.biz.mail.ne1.yahoo.com> <9BF995BC0E47744E9673A41486E24EE25845D838DD@MERCERMAIL.MercerU.local>, <761E2B5697F795489C8710BCC72141FF0B534C@ex07.net.ucsf.edu> Message-ID: I know the potential for damage to your health is huge in histology, but are there any studies out there that indicate histotechs are less healthy than the average person? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Tuesday, December 03, 2013 7:06 PM To: Histonet Subject: [Histonet] RE: Yahoo link Old Histologists never die, they're just well fixed... Claire ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Morken, Timothy [Timothy.Morken@ucsfmedctr.org] Sent: Tuesday, December 03, 2013 11:22 AM To: 'Shirley A. Powell'; Histonet Subject: [Histonet] RE: Yahoo link Well, Shirley, you are actually an Angel, so nothing will ever stop you!! (from an old Georgia Society hand). Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shirley A. Powell Sent: Tuesday, December 03, 2013 9:18 AM To: Histonet Subject: [Histonet] Yahoo link When I entered the profession I was told the average life expectancy of a histotechs was 20 years from hiring. That scared me but I was already hooked. I have been doing this 51 years, so maybe good laboratory practices can help, in spite of bad ventilation and all those other dangers mentioned. Shirley Powell Antique Histotech -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Tuesday, December 03, 2013 11:40 AM To: Morken, Timothy; Histonet Subject: Re: [Histonet] RE: Yahoo link Just what I was thinking. We will never get enough new students now! With proper training and equipment, we do the job safely! And you can drown in an inch of water. Is that in the MSDS! Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 5830 N Blue Lake Dr. Please note new address! Norman, OK 73069 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ________________________________ From: "Morken, Timothy" To: 'Mike Tighe' ; "histonet@lists.utsouthwestern.edu (histonet@lists.utsouthwestern.edu)" Sent: Tuesday, December 3, 2013 10:24 AM Subject: [Histonet] RE: Yahoo link Great. Just what we need. Even so, we in the business can take these lists with a grain of K4[Fe(CN) 6] * 3H 2 O since we know that suitable precautions preclude most of the danger. For instance, I'll have to say that the histo lab here is wonderful in that it has such good ventilation (ie., extraction) that there is none of the usual chemical smell - no xylene, alcohol, specials chemicals that often assaults the senses in histology. Vendors that come here are amazed. Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Tighe Sent: Tuesday, December 03, 2013 6:31 AM To: histonet@lists.utsouthwestern.edu (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Yahoo link Anybody wonder who has the most harzardous job to your health? We're Number One!!! http://finance.yahoo.com/news/the-15-jobs-that-are-most-damaging-to-your-health-155706120.html Mike _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as possible. From lpwenk <@t> sbcglobal.net Wed Dec 4 07:41:14 2013 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Wed Dec 4 07:41:18 2013 Subject: [Histonet] Histology Leader Webinars Message-ID: <48CA3946A25A4691A915DFA011F0D599@HP2010> If not interested in webinars designed for supervisors and trainers/instructors, please delete. NSH has developed a new set of webinars for supervisors and instructors. 11 in total in 2014 ? 3 on management, 3 on education/training, 5 on quality. The webinars are $35 per person if ordered individually. Discounts: If ordering: - all 11 = $325 - 3 management = $90 - 3 education = $90 - 5 quality = $145 - pick any 6 = $180 1 hour each, 1 hour CE if attend the webinar (will get an archive version later for reference, but cannot attend later and earn CE). 1-2 pm ET, various days of the week. For more information: http://www.nsh.org/content/2014-histology-leader-webinars Click on ?Click here to register now? On top, click on ?Webinars? to get titles, speakers, abstracts, dates Disclosure: I?m the NSH webinar coordinator. Non-paid position. Just working with NSH to bring educational material to supervisors and instructors in histotechnology. Peggy A. Wenk, HTL(ASCP)SLS From anolan <@t> prometheushealthcare.com Wed Dec 4 07:49:53 2013 From: anolan <@t> prometheushealthcare.com (anolan@prometheushealthcare.com) Date: Wed Dec 4 07:48:31 2013 Subject: [Histonet] Looking for histotechnologist is NYC Message-ID: <004c01cef0f7$ba874a60$2f95df20$@prometheushealthcare.com> Hi All, I'm currently recruiting for a histotechnologist in NYC. Must have NY State Clinical Laboratory Technologist License and a Bachelor's degree for a 2nd shift opportunity. Please call/email with any questions. Anna Nolan - Recruiter Prometheus Healthcare Direct Line 301-693-8908 Office 301-693-9057 Fax 301-368-2478 anolan @prometheushealthcare.com http://www.linkedin.com/pub/annelise-nolan/55/ba0/ab6 www.prometheushealthcare.com From lpwenk <@t> sbcglobal.net Wed Dec 4 08:23:35 2013 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Wed Dec 4 08:23:40 2013 Subject: [Histonet] RE: Yahoo link In-Reply-To: References: <63f258ee13dc423ab0531cb811d5d71e@BY2PR07MB469.namprd07.prod.outlook.com><761E2B5697F795489C8710BCC72141FF0B52C9@ex07.net.ucsf.edu><1386088821.21321.YahooMailNeo@web5704.biz.mail.ne1.yahoo.com><9BF995BC0E47744E9673A41486E24EE25845D838DD@MERCERMAIL.MercerU.local>, <761E2B5697F795489C8710BCC72141FF0B534C@ex07.net.ucsf.edu> Message-ID: Couple of studies that I know of. One was sponsored by NSH in the mid-1980's. KH Kilburn came to several NSH Symposiums, and did different tests on people who volunteered to participate. Published findings in the late 1980's that said that histotechs had lower pulmonary function than average population, and decreased memory, equilibrium and dexterity than the general population. In Letters to the Editor, people pointed out statistical flaws (low numbers of participants, for example). I also feel there were flaws, such as testing people after traveling over time zones, who were up late at the parties, and had possibly been drinking the night before. There was no way to measure how much exposure to formaldehyde or xylene people were really exposed to. I didn't participate, but if I though the amount I was being exposed to was medium, someone else being exposed to the same amount might have said low amount and someone else could have said high amount. And the studies would say therefore the low pulmonary exposure was due to histotechs being exposed to formaldehyde. But who could say it was due to that chemical, and not due another chemical, or due to the fact that at the same time, people were smoking in the lab I was working in, which was a small space. Another study somewhat relates - S Khattak in 1999 wrote one on pregnancy outcomes following gestational exposure to organic solvents. They interview women who were pregnant and working with organic solvent, so painters for example, so not histotechs only. They compared them to women of same age, same number of children, similar profession not exposed to organic solvents. They found that if the exposed women were having symptoms (breathing problems, rashes), they had a higher percentage of miscarriages and babies with deformities. If there were no symptoms, they had the same, and even lower, percentages than comparable women giving birth who were not exposed. What histology needs is something like the nursing organization in the US has been doing for over 40 years. Everyone who is a member of the nursing society is sent a survey (I think every year), and asked to have physicals information released from their doctors to the organization (It's voluntary to participate). But they have 40+ years of data from hundred of thousands of women, of all ages. They can mine a wealth of medical data from this. NSH (and ASCP) may not have enough histotechs in their organization to pay for the type of survey needed, to continue on for decades. We would need supervisors and bench techs to participate for decades. And probably have to mail them several formaldehyde and xylene monitors every year, or every couple of years, to collect real numbers of how much people were exposed to. And the surveys were pages and pages long (my mother was a nurse who participated from the beginning, so I've seen them), and asked lots of questions about health and diet and smoking and personal issues, in addition to questions about what types of chemical we work with an how much and how often. And how do we measure ventilation in all these places of work? I hope someone comes up with some studies that can help us figure out if histotechs are being exposed to enough chemicals that could be causing these different diseases in humans. There are animal studies, but not human. And remember, people in general, including histotechs, are living longer. If we live longer, we are more likely to have chronic diseases like diabetes, COPD, and cancer. We need to know which cancers are caused by which levels of formaldehyde or organic solvents, vs., say, breast cancer and prostate cancer just because we are women or men. Peggy A. Wenk, HTL(ASCP)SLS -----Original Message----- From: Elizabeth Cameron Sent: Wednesday, December 04, 2013 8:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Yahoo link I know the potential for damage to your health is huge in histology, but are there any studies out there that indicate histotechs are less healthy than the average person? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Tuesday, December 03, 2013 7:06 PM To: Histonet Subject: [Histonet] RE: Yahoo link Old Histologists never die, they're just well fixed... Claire ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Morken, Timothy [Timothy.Morken@ucsfmedctr.org] Sent: Tuesday, December 03, 2013 11:22 AM To: 'Shirley A. Powell'; Histonet Subject: [Histonet] RE: Yahoo link Well, Shirley, you are actually an Angel, so nothing will ever stop you!! (from an old Georgia Society hand). Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shirley A. Powell Sent: Tuesday, December 03, 2013 9:18 AM To: Histonet Subject: [Histonet] Yahoo link When I entered the profession I was told the average life expectancy of a histotechs was 20 years from hiring. That scared me but I was already hooked. I have been doing this 51 years, so maybe good laboratory practices can help, in spite of bad ventilation and all those other dangers mentioned. Shirley Powell Antique Histotech -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Tuesday, December 03, 2013 11:40 AM To: Morken, Timothy; Histonet Subject: Re: [Histonet] RE: Yahoo link Just what I was thinking. We will never get enough new students now! With proper training and equipment, we do the job safely! And you can drown in an inch of water. Is that in the MSDS! Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 5830 N Blue Lake Dr. Please note new address! Norman, OK 73069 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ________________________________ From: "Morken, Timothy" To: 'Mike Tighe' ; "histonet@lists.utsouthwestern.edu (histonet@lists.utsouthwestern.edu)" Sent: Tuesday, December 3, 2013 10:24 AM Subject: [Histonet] RE: Yahoo link Great. Just what we need. Even so, we in the business can take these lists with a grain of K4[Fe(CN) 6] * 3H 2 O since we know that suitable precautions preclude most of the danger. For instance, I'll have to say that the histo lab here is wonderful in that it has such good ventilation (ie., extraction) that there is none of the usual chemical smell - no xylene, alcohol, specials chemicals that often assaults the senses in histology. Vendors that come here are amazed. Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Tighe Sent: Tuesday, December 03, 2013 6:31 AM To: histonet@lists.utsouthwestern.edu (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Yahoo link Anybody wonder who has the most harzardous job to your health? We're Number One!!! http://finance.yahoo.com/news/the-15-jobs-that-are-most-damaging-to-your-health-155706120.html Mike _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as possible. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wsimons <@t> athensgastro.com Wed Dec 4 08:26:48 2013 From: wsimons <@t> athensgastro.com (wsimons@athensgastro.com) Date: Wed Dec 4 08:26:53 2013 Subject: =?utf-8?B?UmU6IFtIaXN0b25ldF0gUkU6IFlhaG9vIGxpbms=?= In-Reply-To: References: <63f258ee13dc423ab0531cb811d5d71e@BY2PR07MB469.namprd07.prod.outlook.com> <761E2B5697F795489C8710BCC72141FF0B52C9@ex07.net.ucsf.edu> <1386088821.21321.YahooMailNeo@web5704.biz.mail.ne1.yahoo.com> <9BF995BC0E47744E9673A41486E24EE25845D838DD@MERCERMAIL.MercerU.local> <761E2B5697F795489C8710BCC72141FF0B534C@ex07.net.ucsf.edu> Message-ID: <20131204142648.27762.qmail@server276.com> I agree! If everyone adheres to safe practices we will make it into our "golden years". Let's bring back our NSH health studies~ Vivian McClure would love for us to continue the studies. Mike Ayers and Shirley Powell have been my mentors through the years (36 plus years for me) Thank you Mike,Shirley, and Billie Swisher....and all my NSH "angels" that paved the way for us "HistoKids" Now everyone take a walk and get a breath of fresh air~ Wanda The Original HistoKid > -------Original Message------- > From: Ingles Claire > To: Histonet > Subject: [Histonet] RE: Yahoo link > Sent: Dec 03 '13 19:05 > > > Old Histologists never die, they're just well fixed... > Claire > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Morken, Timothy [Timothy.Morken@ucsfmedctr.org] > Sent: Tuesday, December 03, 2013 11:22 AM > To: 'Shirley A. Powell'; Histonet > Subject: [Histonet] RE: Yahoo link > > Well, Shirley, you are actually an Angel, so nothing will ever stop you!! (from an old Georgia Society hand). > > Tim Morken > Supervisor, Electron Microscopy and Neuromuscular Special Studies > UC San Francisco Medical Center > San Francisco, CA > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shirley A. Powell > Sent: Tuesday, December 03, 2013 9:18 AM > To: Histonet > Subject: [Histonet] Yahoo link > > When I entered the profession I was told the average life expectancy of a histotechs was 20 years from hiring.??That scared me but I was already hooked.??I have been doing this 51 years, so maybe good laboratory practices can help, in spite of bad ventilation and all those other dangers mentioned. > > Shirley Powell > Antique Histotech > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Pierce > Sent: Tuesday, December 03, 2013 11:40 AM > To: Morken, Timothy; Histonet > Subject: Re: [Histonet] RE: Yahoo link > > Just what I was thinking. We will never get enough new students now! > > With proper training and equipment, we do the job safely! > > > > > And you can drown in an inch of water. Is that in the MSDS! > > Paula K. Pierce, HTL(ASCP)HT > President > Excalibur Pathology, Inc. > 5830 N Blue Lake Dr. Please note new address! > Norman, OK 73069 > 405-759-3953 Lab > 405-759-7513 Fax > www.excaliburpathology.com > > > ________________________________ > From: "Morken, Timothy" > To: 'Mike Tighe' ; "histonet@lists.utsouthwestern.edu (histonet@lists.utsouthwestern.edu)" > Sent: Tuesday, December 3, 2013 10:24 AM > Subject: [Histonet] RE: Yahoo link > > > Great. Just what we need. > > > Even so, we in the business can take these lists with a grain of K4[Fe(CN) 6] * 3H 2 O since we know that suitable precautions preclude most of the danger. For instance, I'll have to say that the histo lab here is wonderful in that it has such good ventilation (ie.,??extraction) that there is none of the usual chemical smell - no xylene, alcohol, specials chemicals that often assaults the senses in histology. Vendors that come here are amazed. > > Tim Morken > Supervisor, Electron Microscopy and Neuromuscular Special Studies > UC San Francisco Medical Center > San Francisco, CA > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Tighe > Sent: Tuesday, December 03, 2013 6:31 AM > To: histonet@lists.utsouthwestern.edu (histonet@lists.utsouthwestern.edu) > Subject: [Histonet] Yahoo link > > Anybody wonder who has the most harzardous job to your health? We're Number One!!! > > > > http://finance.yahoo.com/news/the-15-jobs-that-are-most-damaging-to-your-health-155706120.html > > > > Mike > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From HornHV <@t> archildrens.org Wed Dec 4 08:52:13 2013 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Wed Dec 4 08:52:21 2013 Subject: [Histonet] RE: Yahoo link In-Reply-To: References: <63f258ee13dc423ab0531cb811d5d71e@BY2PR07MB469.namprd07.prod.outlook.com><761E2B5697F795489C8710BCC72141FF0B52C9@ex07.net.ucsf.edu><1386088821.21321.YahooMailNeo@web5704.biz.mail.ne1.yahoo.com><9BF995BC0E47744E9673A41486E24EE25845D838DD@MERCERMAIL.MercerU.local>, <761E2B5697F795489C8710BCC72141FF0B534C@ex07.net.ucsf.edu> Message-ID: <25A4DE08332B19499904459F00AAACB719DA7CAF24@EVS1.archildrens.org> I remember participating in the health study in the 80's. Xylene and Formaldehyde levels are monitored in all labs. If our hospitals/research centers would allow us to forward that information on to someone who could compile data it would be a starting place for a health study. I strongly believe this profession can be danger to health. I can name at least 10 histotechs who have died from cancer. Yes, it may have been when they were older but I feel certain it was from all the chemical exposure in histology before safety became a priority. I know others will remember no ventilation, smoking and eating in the lab, no real requirements for gloves, etc. There were also no MSDS information available and we were exposed through lack of knowledge. I believe the lab is much safer today with all of the safety precautions we take and the knowledge we have on chemicals/stains and their toxicity. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk Sent: Wednesday, December 04, 2013 8:24 AM To: Elizabeth Cameron; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Yahoo link Couple of studies that I know of. One was sponsored by NSH in the mid-1980's. KH Kilburn came to several NSH Symposiums, and did different tests on people who volunteered to participate. Published findings in the late 1980's that said that histotechs had lower pulmonary function than average population, and decreased memory, equilibrium and dexterity than the general population. In Letters to the Editor, people pointed out statistical flaws (low numbers of participants, for example). I also feel there were flaws, such as testing people after traveling over time zones, who were up late at the parties, and had possibly been drinking the night before. There was no way to measure how much exposure to formaldehyde or xylene people were really exposed to. I didn't participate, but if I though the amount I was being exposed to was medium, someone else being exposed to the same amount might have said low amount and someone else could have said high amount. And the studies would say therefore the low pulmonary exposure was due to histotechs being exposed to formaldehyde. But who could say it was due to that chemical, and not due another chemical, or due to the fact that at the same time, people were smoking in the lab I was working in, which was a small space. Another study somewhat relates - S Khattak in 1999 wrote one on pregnancy outcomes following gestational exposure to organic solvents. They interview women who were pregnant and working with organic solvent, so painters for example, so not histotechs only. They compared them to women of same age, same number of children, similar profession not exposed to organic solvents. They found that if the exposed women were having symptoms (breathing problems, rashes), they had a higher percentage of miscarriages and babies with deformities. If there were no symptoms, they had the same, and even lower, percentages than comparable women giving birth who were not exposed. What histology needs is something like the nursing organization in the US has been doing for over 40 years. Everyone who is a member of the nursing society is sent a survey (I think every year), and asked to have physicals information released from their doctors to the organization (It's voluntary to participate). But they have 40+ years of data from hundred of thousands of women, of all ages. They can mine a wealth of medical data from this. NSH (and ASCP) may not have enough histotechs in their organization to pay for the type of survey needed, to continue on for decades. We would need supervisors and bench techs to participate for decades. And probably have to mail them several formaldehyde and xylene monitors every year, or every couple of years, to collect real numbers of how much people were exposed to. And the surveys were pages and pages long (my mother was a nurse who participated from the beginning, so I've seen them), and asked lots of questions about health and diet and smoking and personal issues, in addition to questions about what types of chemical we work with an how much and how often. And how do we measure ventilation in all these places of work? I hope someone comes up with some studies that can help us figure out if histotechs are being exposed to enough chemicals that could be causing these different diseases in humans. There are animal studies, but not human. And remember, people in general, including histotechs, are living longer. If we live longer, we are more likely to have chronic diseases like diabetes, COPD, and cancer. We need to know which cancers are caused by which levels of formaldehyde or organic solvents, vs., say, breast cancer and prostate cancer just because we are women or men. Peggy A. Wenk, HTL(ASCP)SLS -----Original Message----- From: Elizabeth Cameron Sent: Wednesday, December 04, 2013 8:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Yahoo link I know the potential for damage to your health is huge in histology, but are there any studies out there that indicate histotechs are less healthy than the average person? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Tuesday, December 03, 2013 7:06 PM To: Histonet Subject: [Histonet] RE: Yahoo link Old Histologists never die, they're just well fixed... Claire ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Morken, Timothy [Timothy.Morken@ucsfmedctr.org] Sent: Tuesday, December 03, 2013 11:22 AM To: 'Shirley A. Powell'; Histonet Subject: [Histonet] RE: Yahoo link Well, Shirley, you are actually an Angel, so nothing will ever stop you!! (from an old Georgia Society hand). Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shirley A. Powell Sent: Tuesday, December 03, 2013 9:18 AM To: Histonet Subject: [Histonet] Yahoo link When I entered the profession I was told the average life expectancy of a histotechs was 20 years from hiring. That scared me but I was already hooked. I have been doing this 51 years, so maybe good laboratory practices can help, in spite of bad ventilation and all those other dangers mentioned. Shirley Powell Antique Histotech -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Tuesday, December 03, 2013 11:40 AM To: Morken, Timothy; Histonet Subject: Re: [Histonet] RE: Yahoo link Just what I was thinking. We will never get enough new students now! With proper training and equipment, we do the job safely! And you can drown in an inch of water. Is that in the MSDS! Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 5830 N Blue Lake Dr. Please note new address! Norman, OK 73069 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ________________________________ From: "Morken, Timothy" To: 'Mike Tighe' ; "histonet@lists.utsouthwestern.edu (histonet@lists.utsouthwestern.edu)" Sent: Tuesday, December 3, 2013 10:24 AM Subject: [Histonet] RE: Yahoo link Great. Just what we need. Even so, we in the business can take these lists with a grain of K4[Fe(CN) 6] * 3H 2 O since we know that suitable precautions preclude most of the danger. For instance, I'll have to say that the histo lab here is wonderful in that it has such good ventilation (ie., extraction) that there is none of the usual chemical smell - no xylene, alcohol, specials chemicals that often assaults the senses in histology. Vendors that come here are amazed. Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Tighe Sent: Tuesday, December 03, 2013 6:31 AM To: histonet@lists.utsouthwestern.edu (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Yahoo link Anybody wonder who has the most harzardous job to your health? We're Number One!!! http://finance.yahoo.com/news/the-15-jobs-that-are-most-damaging-to-your-health-155706120.html Mike _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as possible. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** This message is confidential, intended only for the named recipient(s) and may contain information that is privileged, attorney work product, highly confidential, or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender by reply electronic mail, delete this electronic mail from your computer, and destroy any copies in any form immediately. Receipt by anyone other than the named recipient(s) is not a waiver of any attorney-client, work product, or other applicable privilege. From mucram11 <@t> comcast.net Wed Dec 4 09:07:12 2013 From: mucram11 <@t> comcast.net (Pam Marcum) Date: Wed Dec 4 09:07:40 2013 Subject: [Histonet] RE: Yahoo link In-Reply-To: <25A4DE08332B19499904459F00AAACB719DA7CAF24@EVS1.archildrens.org> Message-ID: <1979444625.2069003.1386169632659.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> I remember the study and some fairly important people in NSH at the time thinking it was a little overblown.? It was not followed up on at the time just a report in JOH and done.? I have been in Histology many years (about 50) and like Hazel have seen many of the ones even older than us die of cancer or organ failure.? Histology is safer than it has ever been however; I am not sure people really pay enough attention to the dangers with chemicals and tissue that is not well fixed.? I remember people smoking in labs, eating (even in the autopsy suite) and having a cup of coffee or soda at the microtome.? One of my pathologists told us to clean paraffin off our hands with xylene like he did.? We have come a long way just not far enough.? Pam Marcum ----- Original Message ----- From: "Hazel V Horn" To: "Lee & Peggy Wenk" , "Elizabeth Cameron" , histonet@lists.utsouthwestern.edu Sent: Wednesday, December 4, 2013 8:52:13 AM Subject: RE: [Histonet] RE: Yahoo link I remember participating in the health study in the 80's. ? Xylene and Formaldehyde levels are monitored in all labs. ?If our hospitals/research centers would allow us to forward that information on to someone who could compile data it would be a starting place for a health study. I strongly believe this profession can be danger to health. ?I can name at least 10 histotechs who have died from cancer. ?Yes, it may have been when they were older but I feel certain it was from all the chemical exposure in histology before safety became a priority. ? I know others will remember no ventilation, smoking and eating in the lab, no real requirements for gloves, etc. ? ? There were also no MSDS information available and we were exposed through lack of knowledge. I believe the lab is much safer today with all of the safety precautions we take and the knowledge we have on chemicals/stains and their toxicity. ? Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk Sent: Wednesday, December 04, 2013 8:24 AM To: Elizabeth Cameron; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Yahoo link Couple of studies that I know of. One was sponsored by NSH in the mid-1980's. KH Kilburn came to several NSH Symposiums, and did different tests on people who volunteered to participate. Published findings in the late 1980's that said that histotechs had lower pulmonary function than average population, and decreased memory, equilibrium and dexterity than the general population. In Letters to the Editor, people pointed out statistical flaws (low numbers of participants, for example). I also feel there were flaws, such as testing people after traveling over time zones, who were up late at the parties, and had possibly been drinking the night before. There was no way to measure how much exposure to formaldehyde or xylene people were really exposed to. I didn't participate, but if I though the amount I was being exposed to was medium, someone else being exposed to the same amount might have said low amount and someone else could have said high amount. And the studies would say therefore the low pulmonary exposure was due to histotechs being exposed to formaldehyde. But who could say it was due to that chemical, and not due another chemical, or due to the fact that at the same time, people were smoking in the lab I was working in, which was a small space. Another study somewhat relates - S Khattak in 1999 wrote one on pregnancy outcomes following gestational exposure to organic solvents. They interview women who were pregnant and working with organic solvent, so painters for example, so not histotechs only. They compared them to women of same age, same number of children, similar profession not exposed to organic solvents. They found that if the exposed women were having symptoms (breathing problems, rashes), they had a higher percentage of miscarriages and babies with deformities. If there were no symptoms, they had the same, and even lower, percentages than comparable women giving birth who were not exposed. What histology needs is something like the nursing organization in the US has been doing for over 40 years. Everyone who is a member of the nursing society is sent a survey (I think every year), and asked to have physicals information released from their doctors to the organization (It's voluntary to participate). But they have 40+ years of data from hundred of thousands of women, of all ages. They can mine a wealth of medical data from this. NSH (and ASCP) may not have enough histotechs in their organization to pay for the type of survey needed, to continue on for decades. We would need supervisors and bench techs to participate for decades. And probably have to mail them several formaldehyde and xylene monitors every year, or every couple of years, to collect real numbers of how much people were exposed to. And the surveys were pages and pages long (my mother was a nurse who participated from the beginning, so I've seen them), and asked lots of questions about health and diet and smoking and personal issues, in addition to questions about what types of chemical we work with an how much and how often. And how do we measure ventilation in all these places of work? I hope someone comes up with some studies that can help us figure out if histotechs are being exposed to enough chemicals that could be causing these different diseases in humans. There are animal studies, but not human. And remember, people in general, including histotechs, are living longer. If we live longer, we are more likely to have chronic diseases like diabetes, COPD, and cancer. We need to know which cancers are caused by which levels of formaldehyde or organic solvents, vs., say, breast cancer and prostate cancer just because we are women or men. Peggy A. Wenk, HTL(ASCP)SLS -----Original Message----- From: Elizabeth Cameron Sent: Wednesday, December 04, 2013 8:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Yahoo link I know the potential for damage to your health is huge in histology, but are there any studies out there that indicate histotechs are less healthy than the average person? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Tuesday, December 03, 2013 7:06 PM To: Histonet Subject: [Histonet] RE: Yahoo link Old Histologists never die, they're just well fixed... Claire ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Morken, Timothy [Timothy.Morken@ucsfmedctr.org] Sent: Tuesday, December 03, 2013 11:22 AM To: 'Shirley A. Powell'; Histonet Subject: [Histonet] RE: Yahoo link Well, Shirley, you are actually an Angel, so nothing will ever stop you!! (from an old Georgia Society hand). Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shirley A. Powell Sent: Tuesday, December 03, 2013 9:18 AM To: Histonet Subject: [Histonet] Yahoo link When I entered the profession I was told the average life expectancy of a histotechs was 20 years from hiring. ?That scared me but I was already hooked. ?I have been doing this 51 years, so maybe good laboratory practices can help, in spite of bad ventilation and all those other dangers mentioned. Shirley Powell Antique Histotech -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Tuesday, December 03, 2013 11:40 AM To: Morken, Timothy; Histonet Subject: Re: [Histonet] RE: Yahoo link Just what I was thinking. We will never get enough new students now! With proper training and equipment, we do the job safely! And you can drown in an inch of water. Is that in the MSDS! Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 5830 N Blue Lake Dr. Please note new address! Norman, OK 73069 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ________________________________ From: "Morken, Timothy" To: 'Mike Tighe' ; "histonet@lists.utsouthwestern.edu (histonet@lists.utsouthwestern.edu)" Sent: Tuesday, December 3, 2013 10:24 AM Subject: [Histonet] RE: Yahoo link Great. Just what we need. Even so, we in the business can take these lists with a grain of K4[Fe(CN) 6] * 3H 2 O since we know that suitable precautions preclude most of the danger. For instance, I'll have to say that the histo lab here is wonderful in that it has such good ventilation (ie., ?extraction) that there is none of the usual chemical smell - no xylene, alcohol, specials chemicals that often assaults the senses in histology. Vendors that come here are amazed. Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Tighe Sent: Tuesday, December 03, 2013 6:31 AM To: histonet@lists.utsouthwestern.edu (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Yahoo link Anybody wonder who has the most harzardous job to your health? We're Number One!!! http://finance.yahoo.com/news/the-15-jobs-that-are-most-damaging-to-your-health-155706120.html Mike _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as possible. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** This message is confidential, intended only for the named recipient(s) and may contain information that is privileged, attorney work product, highly confidential, or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender by reply electronic mail, delete this electronic mail from your computer, and destroy any copies in any form immediately. Receipt by anyone other than the named recipient(s) is not a waiver of any attorney-client, work product, or other applicable privilege. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akbitting <@t> geisinger.edu Wed Dec 4 09:30:22 2013 From: akbitting <@t> geisinger.edu (Bitting, Angela K.) Date: Wed Dec 4 09:30:29 2013 Subject: [Histonet] RE: Yahoo link In-Reply-To: References: <63f258ee13dc423ab0531cb811d5d71e@BY2PR07MB469.namprd07.prod.outlook.com> <761E2B5697F795489C8710BCC72141FF0B52C9@ex07.net.ucsf.edu> <1386088821.21321.YahooMailNeo@web5704.biz.mail.ne1.yahoo.com> <9BF995BC0E47744E9673A41486E24EE25845D838DD@MERCERMAIL.MercerU.local>, <761E2B5697F795489C8710BCC72141FF0B534C@ex07.net.ucsf.edu> Message-ID: <77F52EFAB8B1694B885E277C48FCD0F64FF07658@GHSEXMBX1W8K1V.geisinger.edu> The article doesn't even mention the repetitive motion injuries. IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. From Joyce.Weems <@t> emoryhealthcare.org Wed Dec 4 09:57:12 2013 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Wed Dec 4 09:57:21 2013 Subject: [Histonet] RE: Yahoo link In-Reply-To: <25A4DE08332B19499904459F00AAACB719DA7CAF24@EVS1.archildrens.org> References: <63f258ee13dc423ab0531cb811d5d71e@BY2PR07MB469.namprd07.prod.outlook.com><761E2B5697F795489C8710BCC72141FF0B52C9@ex07.net.ucsf.edu><1386088821.21321.YahooMailNeo@web5704.biz.mail.ne1.yahoo.com><9BF995BC0E47744E9673A41486E24EE25845D838DD@MERCERMAIL.MercerU.local>, <761E2B5697F795489C8710BCC72141FF0B534C@ex07.net.ucsf.edu> <25A4DE08332B19499904459F00AAACB719DA7CAF24@EVS1.archildrens.org> Message-ID: I worked in a non-ventilated lab once - back in the 70s - just before the xylene study was released. I knew something was wrong and I knew it was xylene and finally was able to get an exhaust put in the window . I could taste it, smell it, and seemed to cough it up!! And my brain was fuzzier than my usual fuzz! The lab director's office was next to my lab and she moved, because she said she couldn't stand to be next to me! So thankful for good regs now and labs since then that have been very well ventilated. We have come a long way, baby!! Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Wednesday, December 04, 2013 9:52 AM To: 'Lee & Peggy Wenk'; Elizabeth Cameron; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Yahoo link I remember participating in the health study in the 80's. Xylene and Formaldehyde levels are monitored in all labs. If our hospitals/research centers would allow us to forward that information on to someone who could compile data it would be a starting place for a health study. I strongly believe this profession can be danger to health. I can name at least 10 histotechs who have died from cancer. Yes, it may have been when they were older but I feel certain it was from all the chemical exposure in histology before safety became a priority. I know others will remember no ventilation, smoking and eating in the lab, no real requirements for gloves, etc. There were also no MSDS information available and we were exposed through lack of knowledge. I believe the lab is much safer today with all of the safety precautions we take and the knowledge we have on chemicals/stains and their toxicity. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk Sent: Wednesday, December 04, 2013 8:24 AM To: Elizabeth Cameron; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Yahoo link Couple of studies that I know of. One was sponsored by NSH in the mid-1980's. KH Kilburn came to several NSH Symposiums, and did different tests on people who volunteered to participate. Published findings in the late 1980's that said that histotechs had lower pulmonary function than average population, and decreased memory, equilibrium and dexterity than the general population. In Letters to the Editor, people pointed out statistical flaws (low numbers of participants, for example). I also feel there were flaws, such as testing people after traveling over time zones, who were up late at the parties, and had possibly been drinking the night before. There was no way to measure how much exposure to formaldehyde or xylene people were really exposed to. I didn't participate, but if I though the amount I was being exposed to was medium, someone else being exposed to the same amount might have said low amount and someone else could have said high amount. And the studies would say therefore the low pulmonary exposure was due to histotechs being exposed to formaldehyde. But who could say it was due to that chemical, and not due another chemical, or due to the fact that at the same time, people were smoking in the lab I was working in, which was a small space. Another study somewhat relates - S Khattak in 1999 wrote one on pregnancy outcomes following gestational exposure to organic solvents. They interview women who were pregnant and working with organic solvent, so painters for example, so not histotechs only. They compared them to women of same age, same number of children, similar profession not exposed to organic solvents. They found that if the exposed women were having symptoms (breathing problems, rashes), they had a higher percentage of miscarriages and babies with deformities. If there were no symptoms, they had the same, and even lower, percentages than comparable women giving birth who were not exposed. What histology needs is something like the nursing organization in the US has been doing for over 40 years. Everyone who is a member of the nursing society is sent a survey (I think every year), and asked to have physicals information released from their doctors to the organization (It's voluntary to participate). But they have 40+ years of data from hundred of thousands of women, of all ages. They can mine a wealth of medical data from this. NSH (and ASCP) may not have enough histotechs in their organization to pay for the type of survey needed, to continue on for decades. We would need supervisors and bench techs to participate for decades. And probably have to mail them several formaldehyde and xylene monitors every year, or every couple of years, to collect real numbers of how much people were exposed to. And the surveys were pages and pages long (my mother was a nurse who participated from the beginning, so I've seen them), and asked lots of questions about health and diet and smoking and personal issues, in addition to questions about what types of chemical we work with an how much and how often. And how do we measure ventilation in all these places of work? I hope someone comes up with some studies that can help us figure out if histotechs are being exposed to enough chemicals that could be causing these different diseases in humans. There are animal studies, but not human. And remember, people in general, including histotechs, are living longer. If we live longer, we are more likely to have chronic diseases like diabetes, COPD, and cancer. We need to know which cancers are caused by which levels of formaldehyde or organic solvents, vs., say, breast cancer and prostate cancer just because we are women or men. Peggy A. Wenk, HTL(ASCP)SLS -----Original Message----- From: Elizabeth Cameron Sent: Wednesday, December 04, 2013 8:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Yahoo link I know the potential for damage to your health is huge in histology, but are there any studies out there that indicate histotechs are less healthy than the average person? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Tuesday, December 03, 2013 7:06 PM To: Histonet Subject: [Histonet] RE: Yahoo link Old Histologists never die, they're just well fixed... Claire ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Morken, Timothy [Timothy.Morken@ucsfmedctr.org] Sent: Tuesday, December 03, 2013 11:22 AM To: 'Shirley A. Powell'; Histonet Subject: [Histonet] RE: Yahoo link Well, Shirley, you are actually an Angel, so nothing will ever stop you!! (from an old Georgia Society hand). Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shirley A. Powell Sent: Tuesday, December 03, 2013 9:18 AM To: Histonet Subject: [Histonet] Yahoo link When I entered the profession I was told the average life expectancy of a histotechs was 20 years from hiring. That scared me but I was already hooked. I have been doing this 51 years, so maybe good laboratory practices can help, in spite of bad ventilation and all those other dangers mentioned. Shirley Powell Antique Histotech -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Tuesday, December 03, 2013 11:40 AM To: Morken, Timothy; Histonet Subject: Re: [Histonet] RE: Yahoo link Just what I was thinking. We will never get enough new students now! With proper training and equipment, we do the job safely! And you can drown in an inch of water. Is that in the MSDS! Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 5830 N Blue Lake Dr. Please note new address! Norman, OK 73069 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ________________________________ From: "Morken, Timothy" To: 'Mike Tighe' ; "histonet@lists.utsouthwestern.edu (histonet@lists.utsouthwestern.edu)" Sent: Tuesday, December 3, 2013 10:24 AM Subject: [Histonet] RE: Yahoo link Great. Just what we need. Even so, we in the business can take these lists with a grain of K4[Fe(CN) 6] * 3H 2 O since we know that suitable precautions preclude most of the danger. For instance, I'll have to say that the histo lab here is wonderful in that it has such good ventilation (ie., extraction) that there is none of the usual chemical smell - no xylene, alcohol, specials chemicals that often assaults the senses in histology. Vendors that come here are amazed. Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Tighe Sent: Tuesday, December 03, 2013 6:31 AM To: histonet@lists.utsouthwestern.edu (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Yahoo link Anybody wonder who has the most harzardous job to your health? We're Number One!!! http://finance.yahoo.com/news/the-15-jobs-that-are-most-damaging-to-your-health-155706120.html Mike _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as possible. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** This message is confidential, intended only for the named recipient(s) and may contain information that is privileged, attorney work product, highly confidential, or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender by reply electronic mail, delete this electronic mail from your computer, and destroy any copies in any form immediately. Receipt by anyone other than the named recipient(s) is not a waiver of any attorney-client, work product, or other applicable privilege. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From POWELL_SA <@t> mercer.edu Wed Dec 4 10:41:41 2013 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Wed Dec 4 10:41:48 2013 Subject: [Histonet] RE: Yahoo link In-Reply-To: References: <63f258ee13dc423ab0531cb811d5d71e@BY2PR07MB469.namprd07.prod.outlook.com><761E2B5697F795489C8710BCC72141FF0B52C9@ex07.net.ucsf.edu><1386088821.21321.YahooMailNeo@web5704.biz.mail.ne1.yahoo.com><9BF995BC0E47744E9673A41486E24EE25845D838DD@MERCERMAIL.MercerU.local>, <761E2B5697F795489C8710BCC72141FF0B534C@ex07.net.ucsf.edu> <25A4DE08332B19499904459F00AAACB719DA7CAF24@EVS1.archildrens.org> Message-ID: <9BF995BC0E47744E9673A41486E24EE25845D83E22@MERCERMAIL.MercerU.local> I had the same experience, the clinical lab techs asked the supervisor to keep our door closed so they would not have to smell the fumes, just lock us up in it. I was considering calling EPA in to check it out. Thank goodness those days are gone. Use those fume hoods and all the other ppe you can get. :) Shirley Powell -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Wednesday, December 04, 2013 10:57 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Yahoo link I worked in a non-ventilated lab once - back in the 70s - just before the xylene study was released. I knew something was wrong and I knew it was xylene and finally was able to get an exhaust put in the window . I could taste it, smell it, and seemed to cough it up!! And my brain was fuzzier than my usual fuzz! The lab director's office was next to my lab and she moved, because she said she couldn't stand to be next to me! So thankful for good regs now and labs since then that have been very well ventilated. We have come a long way, baby!! Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Wednesday, December 04, 2013 9:52 AM To: 'Lee & Peggy Wenk'; Elizabeth Cameron; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Yahoo link I remember participating in the health study in the 80's. Xylene and Formaldehyde levels are monitored in all labs. If our hospitals/research centers would allow us to forward that information on to someone who could compile data it would be a starting place for a health study. I strongly believe this profession can be danger to health. I can name at least 10 histotechs who have died from cancer. Yes, it may have been when they were older but I feel certain it was from all the chemical exposure in histology before safety became a priority. I know others will remember no ventilation, smoking and eating in the lab, no real requirements for gloves, etc. There were also no MSDS information available and we were exposed through lack of knowledge. I believe the lab is much safer today with all of the safety precautions we take and the knowledge we have on chemicals/stains and their toxicity. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk Sent: Wednesday, December 04, 2013 8:24 AM To: Elizabeth Cameron; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Yahoo link Couple of studies that I know of. One was sponsored by NSH in the mid-1980's. KH Kilburn came to several NSH Symposiums, and did different tests on people who volunteered to participate. Published findings in the late 1980's that said that histotechs had lower pulmonary function than average population, and decreased memory, equilibrium and dexterity than the general population. In Letters to the Editor, people pointed out statistical flaws (low numbers of participants, for example). I also feel there were flaws, such as testing people after traveling over time zones, who were up late at the parties, and had possibly been drinking the night before. There was no way to measure how much exposure to formaldehyde or xylene people were really exposed to. I didn't participate, but if I though the amount I was being exposed to was medium, someone else being exposed to the same amount might have said low amount and someone else could have said high amount. And the studies would say therefore the low pulmonary exposure was due to histotechs being exposed to formaldehyde. But who could say it was due to that chemical, and not due another chemical, or due to the fact that at the same time, people were smoking in the lab I was working in, which was a small space. Another study somewhat relates - S Khattak in 1999 wrote one on pregnancy outcomes following gestational exposure to organic solvents. They interview women who were pregnant and working with organic solvent, so painters for example, so not histotechs only. They compared them to women of same age, same number of children, similar profession not exposed to organic solvents. They found that if the exposed women were having symptoms (breathing problems, rashes), they had a higher percentage of miscarriages and babies with deformities. If there were no symptoms, they had the same, and even lower, percentages than comparable women giving birth who were not exposed. What histology needs is something like the nursing organization in the US has been doing for over 40 years. Everyone who is a member of the nursing society is sent a survey (I think every year), and asked to have physicals information released from their doctors to the organization (It's voluntary to participate). But they have 40+ years of data from hundred of thousands of women, of all ages. They can mine a wealth of medical data from this. NSH (and ASCP) may not have enough histotechs in their organization to pay for the type of survey needed, to continue on for decades. We would need supervisors and bench techs to participate for decades. And probably have to mail them several formaldehyde and xylene monitors every year, or every couple of years, to collect real numbers of how much people were exposed to. And the surveys were pages and pages long (my mother was a nurse who participated from the beginning, so I've seen them), and asked lots of questions about health and diet and smoking and personal issues, in addition to questions about what types of chemical we work with an how much and how often. And how do we measure ventilation in all these places of work? I hope someone comes up with some studies that can help us figure out if histotechs are being exposed to enough chemicals that could be causing these different diseases in humans. There are animal studies, but not human. And remember, people in general, including histotechs, are living longer. If we live longer, we are more likely to have chronic diseases like diabetes, COPD, and cancer. We need to know which cancers are caused by which levels of formaldehyde or organic solvents, vs., say, breast cancer and prostate cancer just because we are women or men. Peggy A. Wenk, HTL(ASCP)SLS -----Original Message----- From: Elizabeth Cameron Sent: Wednesday, December 04, 2013 8:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Yahoo link I know the potential for damage to your health is huge in histology, but are there any studies out there that indicate histotechs are less healthy than the average person? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Tuesday, December 03, 2013 7:06 PM To: Histonet Subject: [Histonet] RE: Yahoo link Old Histologists never die, they're just well fixed... Claire ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Morken, Timothy [Timothy.Morken@ucsfmedctr.org] Sent: Tuesday, December 03, 2013 11:22 AM To: 'Shirley A. Powell'; Histonet Subject: [Histonet] RE: Yahoo link Well, Shirley, you are actually an Angel, so nothing will ever stop you!! (from an old Georgia Society hand). Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shirley A. Powell Sent: Tuesday, December 03, 2013 9:18 AM To: Histonet Subject: [Histonet] Yahoo link When I entered the profession I was told the average life expectancy of a histotechs was 20 years from hiring. That scared me but I was already hooked. I have been doing this 51 years, so maybe good laboratory practices can help, in spite of bad ventilation and all those other dangers mentioned. Shirley Powell Antique Histotech -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Tuesday, December 03, 2013 11:40 AM To: Morken, Timothy; Histonet Subject: Re: [Histonet] RE: Yahoo link Just what I was thinking. We will never get enough new students now! With proper training and equipment, we do the job safely! And you can drown in an inch of water. Is that in the MSDS! Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 5830 N Blue Lake Dr. Please note new address! Norman, OK 73069 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ________________________________ From: "Morken, Timothy" To: 'Mike Tighe' ; "histonet@lists.utsouthwestern.edu (histonet@lists.utsouthwestern.edu)" Sent: Tuesday, December 3, 2013 10:24 AM Subject: [Histonet] RE: Yahoo link Great. Just what we need. Even so, we in the business can take these lists with a grain of K4[Fe(CN) 6] * 3H 2 O since we know that suitable precautions preclude most of the danger. For instance, I'll have to say that the histo lab here is wonderful in that it has such good ventilation (ie., extraction) that there is none of the usual chemical smell - no xylene, alcohol, specials chemicals that often assaults the senses in histology. Vendors that come here are amazed. Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Tighe Sent: Tuesday, December 03, 2013 6:31 AM To: histonet@lists.utsouthwestern.edu (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Yahoo link Anybody wonder who has the most harzardous job to your health? We're Number One!!! http://finance.yahoo.com/news/the-15-jobs-that-are-most-damaging-to-your-health-155706120.html Mike _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as possible. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** This message is confidential, intended only for the named recipient(s) and may contain information that is privileged, attorney work product, highly confidential, or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender by reply electronic mail, delete this electronic mail from your computer, and destroy any copies in any form immediately. Receipt by anyone other than the named recipient(s) is not a waiver of any attorney-client, work product, or other applicable privilege. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tpodawiltz <@t> lrgh.org Wed Dec 4 11:17:05 2013 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Wed Dec 4 11:17:14 2013 Subject: [Histonet] RE: Yahoo link NO PHI In-Reply-To: <9BF995BC0E47744E9673A41486E24EE25845D83E22@MERCERMAIL.MercerU.local> References: <63f258ee13dc423ab0531cb811d5d71e@BY2PR07MB469.namprd07.prod.outlook.com><761E2B5697F795489C8710BCC72141FF0B52C9@ex07.net.ucsf.edu><1386088821.21321.YahooMailNeo@web5704.biz.mail.ne1.yahoo.com><9BF995BC0E47744E9673A41486E24EE25845D838DD@MERCERMAIL.MercerU.local>, <761E2B5697F795489C8710BCC72141FF0B534C@ex07.net.ucsf.edu> <25A4DE08332B19499904459F00AAACB719DA7CAF24@EVS1.archildrens.org> <9BF995BC0E47744E9673A41486E24EE25845D83E22@MERCERMAIL.MercerU.local> Message-ID: <38667E7FB77ECD4E91BFAEB8D986386325011F302B@LRGHEXVS1.practice.lrgh.org> My current lab the air exchange rate is 58/hr. The good news: you do not smell fumes of any kind. The bad news: we are negative pressure and the unvented bathrooms are just outside our lab. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shirley A. Powell Sent: Wednesday, December 04, 2013 11:42 AM To: Weems, Joyce K.; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Yahoo link I had the same experience, the clinical lab techs asked the supervisor to keep our door closed so they would not have to smell the fumes, just lock us up in it. I was considering calling EPA in to check it out. Thank goodness those days are gone. Use those fume hoods and all the other ppe you can get. :) Shirley Powell -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Wednesday, December 04, 2013 10:57 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Yahoo link I worked in a non-ventilated lab once - back in the 70s - just before the xylene study was released. I knew something was wrong and I knew it was xylene and finally was able to get an exhaust put in the window . I could taste it, smell it, and seemed to cough it up!! And my brain was fuzzier than my usual fuzz! The lab director's office was next to my lab and she moved, because she said she couldn't stand to be next to me! So thankful for good regs now and labs since then that have been very well ventilated. We have come a long way, baby!! Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Wednesday, December 04, 2013 9:52 AM To: 'Lee & Peggy Wenk'; Elizabeth Cameron; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Yahoo link I remember participating in the health study in the 80's. Xylene and Formaldehyde levels are monitored in all labs. If our hospitals/research centers would allow us to forward that information on to someone who could compile data it would be a starting place for a health study. I strongly believe this profession can be danger to health. I can name at least 10 histotechs who have died from cancer. Yes, it may have been when they were older but I feel certain it was from all the chemical exposure in histology before safety became a priority. I know others will remember no ventilation, smoking and eating in the lab, no real requirements for gloves, etc. There were also no MSDS information available and we were exposed through lack of knowledge. I believe the lab is much safer today with all of the safety precautions we take and the knowledge we have on chemicals/stains and their toxicity. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk Sent: Wednesday, December 04, 2013 8:24 AM To: Elizabeth Cameron; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Yahoo link Couple of studies that I know of. One was sponsored by NSH in the mid-1980's. KH Kilburn came to several NSH Symposiums, and did different tests on people who volunteered to participate. Published findings in the late 1980's that said that histotechs had lower pulmonary function than average population, and decreased memory, equilibrium and dexterity than the general population. In Letters to the Editor, people pointed out statistical flaws (low numbers of participants, for example). I also feel there were flaws, such as testing people after traveling over time zones, who were up late at the parties, and had possibly been drinking the night before. There was no way to measure how much exposure to formaldehyde or xylene people were really exposed to. I didn't participate, but if I though the amount I was being exposed to was medium, someone else being exposed to the same amount might have said low amount and someone else could have said high amount. And the studies would say therefore the low pulmonary exposure was due to histotechs being exposed to formaldehyde. But who could say it was due to that chemical, and not due another chemical, or due to the fact that at the same time, people were smoking in the lab I was working in, which was a small space. Another study somewhat relates - S Khattak in 1999 wrote one on pregnancy outcomes following gestational exposure to organic solvents. They interview women who were pregnant and working with organic solvent, so painters for example, so not histotechs only. They compared them to women of same age, same number of children, similar profession not exposed to organic solvents. They found that if the exposed women were having symptoms (breathing problems, rashes), they had a higher percentage of miscarriages and babies with deformities. If there were no symptoms, they had the same, and even lower, percentages than comparable women giving birth who were not exposed. What histology needs is something like the nursing organization in the US has been doing for over 40 years. Everyone who is a member of the nursing society is sent a survey (I think every year), and asked to have physicals information released from their doctors to the organization (It's voluntary to participate). But they have 40+ years of data from hundred of thousands of women, of all ages. They can mine a wealth of medical data from this. NSH (and ASCP) may not have enough histotechs in their organization to pay for the type of survey needed, to continue on for decades. We would need supervisors and bench techs to participate for decades. And probably have to mail them several formaldehyde and xylene monitors every year, or every couple of years, to collect real numbers of how much people were exposed to. And the surveys were pages and pages long (my mother was a nurse who participated from the beginning, so I've seen them), and asked lots of questions about health and diet and smoking and personal issues, in addition to questions about what types of chemical we work with an how much and how often. And how do we measure ventilation in all these places of work? I hope someone comes up with some studies that can help us figure out if histotechs are being exposed to enough chemicals that could be causing these different diseases in humans. There are animal studies, but not human. And remember, people in general, including histotechs, are living longer. If we live longer, we are more likely to have chronic diseases like diabetes, COPD, and cancer. We need to know which cancers are caused by which levels of formaldehyde or organic solvents, vs., say, breast cancer and prostate cancer just because we are women or men. Peggy A. Wenk, HTL(ASCP)SLS -----Original Message----- From: Elizabeth Cameron Sent: Wednesday, December 04, 2013 8:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Yahoo link I know the potential for damage to your health is huge in histology, but are there any studies out there that indicate histotechs are less healthy than the average person? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Tuesday, December 03, 2013 7:06 PM To: Histonet Subject: [Histonet] RE: Yahoo link Old Histologists never die, they're just well fixed... Claire ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Morken, Timothy [Timothy.Morken@ucsfmedctr.org] Sent: Tuesday, December 03, 2013 11:22 AM To: 'Shirley A. Powell'; Histonet Subject: [Histonet] RE: Yahoo link Well, Shirley, you are actually an Angel, so nothing will ever stop you!! (from an old Georgia Society hand). Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shirley A. Powell Sent: Tuesday, December 03, 2013 9:18 AM To: Histonet Subject: [Histonet] Yahoo link When I entered the profession I was told the average life expectancy of a histotechs was 20 years from hiring. That scared me but I was already hooked. I have been doing this 51 years, so maybe good laboratory practices can help, in spite of bad ventilation and all those other dangers mentioned. Shirley Powell Antique Histotech -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Tuesday, December 03, 2013 11:40 AM To: Morken, Timothy; Histonet Subject: Re: [Histonet] RE: Yahoo link Just what I was thinking. We will never get enough new students now! With proper training and equipment, we do the job safely! And you can drown in an inch of water. Is that in the MSDS! Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 5830 N Blue Lake Dr. Please note new address! Norman, OK 73069 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ________________________________ From: "Morken, Timothy" To: 'Mike Tighe' ; "histonet@lists.utsouthwestern.edu (histonet@lists.utsouthwestern.edu)" Sent: Tuesday, December 3, 2013 10:24 AM Subject: [Histonet] RE: Yahoo link Great. Just what we need. Even so, we in the business can take these lists with a grain of K4[Fe(CN) 6] * 3H 2 O since we know that suitable precautions preclude most of the danger. For instance, I'll have to say that the histo lab here is wonderful in that it has such good ventilation (ie., extraction) that there is none of the usual chemical smell - no xylene, alcohol, specials chemicals that often assaults the senses in histology. Vendors that come here are amazed. Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Tighe Sent: Tuesday, December 03, 2013 6:31 AM To: histonet@lists.utsouthwestern.edu (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Yahoo link Anybody wonder who has the most harzardous job to your health? We're Number One!!! http://finance.yahoo.com/news/the-15-jobs-that-are-most-damaging-to-your-health-155706120.html Mike _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as possible. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** This message is confidential, intended only for the named recipient(s) and may contain information that is privileged, attorney work product, highly confidential, or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender by reply electronic mail, delete this electronic mail from your computer, and destroy any copies in any form immediately. Receipt by anyone other than the named recipient(s) is not a waiver of any attorney-client, work product, or other applicable privilege. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From Joyce.Weems <@t> emoryhealthcare.org Wed Dec 4 11:18:48 2013 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Wed Dec 4 11:19:09 2013 Subject: [Histonet] RE: Yahoo link NO PHI In-Reply-To: <38667E7FB77ECD4E91BFAEB8D986386325011F302B@LRGHEXVS1.practice.lrgh.org> References: <63f258ee13dc423ab0531cb811d5d71e@BY2PR07MB469.namprd07.prod.outlook.com><761E2B5697F795489C8710BCC72141FF0B52C9@ex07.net.ucsf.edu><1386088821.21321.YahooMailNeo@web5704.biz.mail.ne1.yahoo.com><9BF995BC0E47744E9673A41486E24EE25845D838DD@MERCERMAIL.MercerU.local>, <761E2B5697F795489C8710BCC72141FF0B534C@ex07.net.ucsf.edu> <25A4DE08332B19499904459F00AAACB719DA7CAF24@EVS1.archildrens.org> <9BF995BC0E47744E9673A41486E24EE25845D83E22@MERCERMAIL.MercerU.local> <38667E7FB77ECD4E91BFAEB8D986386325011F302B@LRGHEXVS1.practice.lrgh.org> Message-ID: Oh joy....!!! Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: Podawiltz, Thomas [mailto:tpodawiltz@lrgh.org] Sent: Wednesday, December 04, 2013 12:17 PM To: Shirley A. Powell; Weems, Joyce K.; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Yahoo link NO PHI My current lab the air exchange rate is 58/hr. The good news: you do not smell fumes of any kind. The bad news: we are negative pressure and the unvented bathrooms are just outside our lab. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shirley A. Powell Sent: Wednesday, December 04, 2013 11:42 AM To: Weems, Joyce K.; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Yahoo link I had the same experience, the clinical lab techs asked the supervisor to keep our door closed so they would not have to smell the fumes, just lock us up in it. I was considering calling EPA in to check it out. Thank goodness those days are gone. Use those fume hoods and all the other ppe you can get. :) Shirley Powell -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Wednesday, December 04, 2013 10:57 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Yahoo link I worked in a non-ventilated lab once - back in the 70s - just before the xylene study was released. I knew something was wrong and I knew it was xylene and finally was able to get an exhaust put in the window . I could taste it, smell it, and seemed to cough it up!! And my brain was fuzzier than my usual fuzz! The lab director's office was next to my lab and she moved, because she said she couldn't stand to be next to me! So thankful for good regs now and labs since then that have been very well ventilated. We have come a long way, baby!! Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Wednesday, December 04, 2013 9:52 AM To: 'Lee & Peggy Wenk'; Elizabeth Cameron; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Yahoo link I remember participating in the health study in the 80's. Xylene and Formaldehyde levels are monitored in all labs. If our hospitals/research centers would allow us to forward that information on to someone who could compile data it would be a starting place for a health study. I strongly believe this profession can be danger to health. I can name at least 10 histotechs who have died from cancer. Yes, it may have been when they were older but I feel certain it was from all the chemical exposure in histology before safety became a priority. I know others will remember no ventilation, smoking and eating in the lab, no real requirements for gloves, etc. There were also no MSDS information available and we were exposed through lack of knowledge. I believe the lab is much safer today with all of the safety precautions we take and the knowledge we have on chemicals/stains and their toxicity. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk Sent: Wednesday, December 04, 2013 8:24 AM To: Elizabeth Cameron; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Yahoo link Couple of studies that I know of. One was sponsored by NSH in the mid-1980's. KH Kilburn came to several NSH Symposiums, and did different tests on people who volunteered to participate. Published findings in the late 1980's that said that histotechs had lower pulmonary function than average population, and decreased memory, equilibrium and dexterity than the general population. In Letters to the Editor, people pointed out statistical flaws (low numbers of participants, for example). I also feel there were flaws, such as testing people after traveling over time zones, who were up late at the parties, and had possibly been drinking the night before. There was no way to measure how much exposure to formaldehyde or xylene people were really exposed to. I didn't participate, but if I though the amount I was being exposed to was medium, someone else being exposed to the same amount might have said low amount and someone else could have said high amount. And the studies would say therefore the low pulmonary exposure was due to histotechs being exposed to formaldehyde. But who could say it was due to that chemical, and not due another chemical, or due to the fact that at the same time, people were smoking in the lab I was working in, which was a small space. Another study somewhat relates - S Khattak in 1999 wrote one on pregnancy outcomes following gestational exposure to organic solvents. They interview women who were pregnant and working with organic solvent, so painters for example, so not histotechs only. They compared them to women of same age, same number of children, similar profession not exposed to organic solvents. They found that if the exposed women were having symptoms (breathing problems, rashes), they had a higher percentage of miscarriages and babies with deformities. If there were no symptoms, they had the same, and even lower, percentages than comparable women giving birth who were not exposed. What histology needs is something like the nursing organization in the US has been doing for over 40 years. Everyone who is a member of the nursing society is sent a survey (I think every year), and asked to have physicals information released from their doctors to the organization (It's voluntary to participate). But they have 40+ years of data from hundred of thousands of women, of all ages. They can mine a wealth of medical data from this. NSH (and ASCP) may not have enough histotechs in their organization to pay for the type of survey needed, to continue on for decades. We would need supervisors and bench techs to participate for decades. And probably have to mail them several formaldehyde and xylene monitors every year, or every couple of years, to collect real numbers of how much people were exposed to. And the surveys were pages and pages long (my mother was a nurse who participated from the beginning, so I've seen them), and asked lots of questions about health and diet and smoking and personal issues, in addition to questions about what types of chemical we work with an how much and how often. And how do we measure ventilation in all these places of work? I hope someone comes up with some studies that can help us figure out if histotechs are being exposed to enough chemicals that could be causing these different diseases in humans. There are animal studies, but not human. And remember, people in general, including histotechs, are living longer. If we live longer, we are more likely to have chronic diseases like diabetes, COPD, and cancer. We need to know which cancers are caused by which levels of formaldehyde or organic solvents, vs., say, breast cancer and prostate cancer just because we are women or men. Peggy A. Wenk, HTL(ASCP)SLS -----Original Message----- From: Elizabeth Cameron Sent: Wednesday, December 04, 2013 8:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Yahoo link I know the potential for damage to your health is huge in histology, but are there any studies out there that indicate histotechs are less healthy than the average person? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Tuesday, December 03, 2013 7:06 PM To: Histonet Subject: [Histonet] RE: Yahoo link Old Histologists never die, they're just well fixed... Claire ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Morken, Timothy [Timothy.Morken@ucsfmedctr.org] Sent: Tuesday, December 03, 2013 11:22 AM To: 'Shirley A. Powell'; Histonet Subject: [Histonet] RE: Yahoo link Well, Shirley, you are actually an Angel, so nothing will ever stop you!! (from an old Georgia Society hand). Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shirley A. Powell Sent: Tuesday, December 03, 2013 9:18 AM To: Histonet Subject: [Histonet] Yahoo link When I entered the profession I was told the average life expectancy of a histotechs was 20 years from hiring. That scared me but I was already hooked. I have been doing this 51 years, so maybe good laboratory practices can help, in spite of bad ventilation and all those other dangers mentioned. Shirley Powell Antique Histotech -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Tuesday, December 03, 2013 11:40 AM To: Morken, Timothy; Histonet Subject: Re: [Histonet] RE: Yahoo link Just what I was thinking. We will never get enough new students now! With proper training and equipment, we do the job safely! And you can drown in an inch of water. Is that in the MSDS! Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 5830 N Blue Lake Dr. Please note new address! Norman, OK 73069 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ________________________________ From: "Morken, Timothy" To: 'Mike Tighe' ; "histonet@lists.utsouthwestern.edu (histonet@lists.utsouthwestern.edu)" Sent: Tuesday, December 3, 2013 10:24 AM Subject: [Histonet] RE: Yahoo link Great. Just what we need. Even so, we in the business can take these lists with a grain of K4[Fe(CN) 6] * 3H 2 O since we know that suitable precautions preclude most of the danger. For instance, I'll have to say that the histo lab here is wonderful in that it has such good ventilation (ie., extraction) that there is none of the usual chemical smell - no xylene, alcohol, specials chemicals that often assaults the senses in histology. Vendors that come here are amazed. Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Tighe Sent: Tuesday, December 03, 2013 6:31 AM To: histonet@lists.utsouthwestern.edu (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Yahoo link Anybody wonder who has the most harzardous job to your health? We're Number One!!! http://finance.yahoo.com/news/the-15-jobs-that-are-most-damaging-to-your-health-155706120.html Mike _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as possible. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** This message is confidential, intended only for the named recipient(s) and may contain information that is privileged, attorney work product, highly confidential, or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender by reply electronic mail, delete this electronic mail from your computer, and destroy any copies in any form immediately. Receipt by anyone other than the named recipient(s) is not a waiver of any attorney-client, work product, or other applicable privilege. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From Timothy.Morken <@t> ucsfmedctr.org Wed Dec 4 11:31:22 2013 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Wed Dec 4 11:31:44 2013 Subject: [Histonet] Rush bx schedule? Message-ID: <761E2B5697F795489C8710BCC72141FF0B56B4@ex07.net.ucsf.edu> For tissue, what step most contributes to subsequent swelling of tissue when the block is soaked? I'm thinking the 100% ETOH, xylene clearing and paraffin infiltration all contribute, but at what percentage? Here is the current schedule, which apparently was meant to mimic a microwave schedule that was used before the MW quit working. The tissue - kidney and liver bx for the most part, up to 10 per day, is coming out soft and swells a bit when soaked. So sectioning is difficult. We need to have a rapid schedule but need good sections. Obviously we are pushing the limits on this so need a bit of leeway for variation in tissue So, I'm considering which of these steps is the most critical to lengthen. This is on a VIP5 but we can also use a peloris for this processing Formalin 2 min, 50degC (this is not necessary at all, IMHO) 80% ETOH 5 min, 50degC 95%ETOH 5 min, 50degC 100% ETOH 8 min, 50degC Xylene, 9 min 50degC Paraffin, 4 min, Paraffin, 5 min Thanks for your insight! Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center Box 1656 505 Parnassus Ave San Francisco, CA 94143 USA 415.353.1266 (office) tim.morken@ucsfmedctr.org From Timothy.Morken <@t> ucsfmedctr.org Wed Dec 4 11:45:43 2013 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Wed Dec 4 11:45:58 2013 Subject: [Histonet] On-slide IHC control workflow? Message-ID: <761E2B5697F795489C8710BCC72141FF0B56E8@ex07.net.ucsf.edu> We are planning our move to using on-slide controls for IHC and I'm wondering how other labs handle the workflow and logistics of matching controls to stain orders. We plan to use a TMA for 80% of our orders. So far we have one TMA that covers most Ab's but the number will probably will be expanded (neuropath-specific, Hempath specific, etc). For those who do this now, how do you handle these tasks: * Do you use TMA or single-tissue controls? Or a mix? * * Cutting all the controls - one or more techs? Part of normal work? Outsource? (especially TMA cutting - inhouse or outsource?) * How do you store the controls? (We plan to cut nearly just-in-time, maybe two days from use). * How do you distribute to the cutters? * How do you Indicate to the cutting techs which control slide to use for a particular stain? (we use over 200 antibodies so need to make as easy as possible without memorization) * How do you prevent the wrong control slide from being used? Anything else we should consider? Thanks for any help!! Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center Box 1656 505 Parnassus Ave San Francisco, CA 94143 USA 415.353.1266 (office) tim.morken@ucsfmedctr.org From cls71877 <@t> gmail.com Wed Dec 4 11:58:15 2013 From: cls71877 <@t> gmail.com (Cristi Rigazio) Date: Wed Dec 4 11:58:27 2013 Subject: [Histonet] Rush bx schedule? In-Reply-To: <761E2B5697F795489C8710BCC72141FF0B56B4@ex07.net.ucsf.edu> References: <761E2B5697F795489C8710BCC72141FF0B56B4@ex07.net.ucsf.edu> Message-ID: <34A6437F-5A98-49D5-AE11-D2AB25D654DD@gmail.com> We do strictly GI biopsies, but if we have a stat case, we have a processing schedule as follows: five minutes in each station of one 70%, two 95%, two 100% and three xylenes, then 10 minutes in each of three paraffins. We have the ASP300 and this has been very successful. I have heard from others they do as little as 2 minutes in each, With a bit longer in the paraffin. Generally our STAT cases are in formalin overnight so we skip that step altogether. Happy Holidays! Cristi Sent from my iPhone On Dec 4, 2013, at 9:31 AM, "Morken, Timothy" wrote: > For tissue, what step most contributes to subsequent swelling of tissue when the block is soaked? I'm thinking the 100% ETOH, xylene clearing and paraffin infiltration all contribute, but at what percentage? > > Here is the current schedule, which apparently was meant to mimic a microwave schedule that was used before the MW quit working. The tissue - kidney and liver bx for the most part, up to 10 per day, is coming out soft and swells a bit when soaked. So sectioning is difficult. > > We need to have a rapid schedule but need good sections. Obviously we are pushing the limits on this so need a bit of leeway for variation in tissue > > So, I'm considering which of these steps is the most critical to lengthen. > This is on a VIP5 but we can also use a peloris for this processing > > Formalin 2 min, 50degC (this is not necessary at all, IMHO) > 80% ETOH 5 min, 50degC > 95%ETOH 5 min, 50degC > 100% ETOH 8 min, 50degC > Xylene, 9 min 50degC > Paraffin, 4 min, > Paraffin, 5 min > > Thanks for your insight! > > > > Tim Morken > Supervisor, Electron Microscopy and Neuromuscular Special Studies > UC San Francisco Medical Center > Box 1656 > 505 Parnassus Ave > San Francisco, CA 94143 > USA > > 415.353.1266 (office) > tim.morken@ucsfmedctr.org > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From chapmanc <@t> health.missouri.edu Wed Dec 4 12:12:10 2013 From: chapmanc <@t> health.missouri.edu (Chapman, Cherie J.) Date: Wed Dec 4 12:12:16 2013 Subject: [Histonet] (no subject) Message-ID: Hello all, I am looking for suggestions on issues with our H&E stain. I supervised a Veterinary Diagnostic lab for over 27 years and produced top quality sections, H&E's, special stains and IHC on a variety of different species in our lab. I am currently working in a Dermatopathology Lab and I am finding inconsistent staining with our H&E's. Working with just skin is a challenge all on its own. We have made changes to our staining protocol and just not happy with the end product. What we are observing is inconsistent staining on levels on the same slide. The top section seems to stain more evenly than the middle and bottom sections. I can actually see three different shades of color. The specimens are ribboned sections so I know it is not from thick and thin sections. We have looked at our processing times, microwave vs. oven times, staining reagents, different brands of hematoxylin and eosin, adjustments on staining times, tap water compared to distilled water. Our main processor is the Thermo Scientific STP-420 and our back up is the Sakura VIP V processor. I have been working with Thermo technical support thinking it might be a processing issue. We have a Leica ST5020 Multstainer/CV5030 Robotic Cover slipper we have made several changes that the technical teams has suggested to the reagents and staining time. It's still not the quality that we are looking for. I have had culligan techs out several times to see if it could be something with the water. We can run 100 slides the same day, same reagents and protocol and the H&E color is so inconsistent. I would appreciate any suggestions in this matter. Cherie Chapman, BS, HT, HTL (ASCP) Associate Director of Dermatopathology Laboratory University of Missouri Department of Dermatology University Physicians Medical Building Phone: (573) 884-0123 Fax: (573) 884-0834 From trathborne <@t> somerset-healthcare.com Wed Dec 4 12:17:01 2013 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Wed Dec 4 12:17:23 2013 Subject: [Histonet] (no subject) In-Reply-To: References: Message-ID: <3AD061FE740D464FAC7BF6B5CFB75707A95A6CE0@SMCMAIL01.somerset-healthcare.com> Could it be the heating/deparaffinization process? If the upper sections are staining more evenly, then maybe they are free from residual paraffin. Try extending the time in the ovens and/or xylene. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Chapman, Cherie J. Sent: Wednesday, December 04, 2013 1:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Hello all, I am looking for suggestions on issues with our H&E stain. I supervised a Veterinary Diagnostic lab for over 27 years and produced top quality sections, H&E's, special stains and IHC on a variety of different species in our lab. I am currently working in a Dermatopathology Lab and I am finding inconsistent staining with our H&E's. Working with just skin is a challenge all on its own. We have made changes to our staining protocol and just not happy with the end product. What we are observing is inconsistent staining on levels on the same slide. The top section seems to stain more evenly than the middle and bottom sections. I can actually see three different shades of color. The specimens are ribboned sections so I know it is not from thick and thin sections. We have looked at our processing times, microwave vs. oven times, staining reagents, different brands of hematoxylin and eosin, adjustments on staining times, tap water compared to distilled water. Our main processor is the Thermo Scientific STP-420 and our back up is the Sakura VIP V processor. I have been working with Thermo technical support thinking it might be a processing issue. We have a Leica ST5020 Multstainer/CV5030 Robotic Cover slipper we have made several changes that the technical teams has suggested to the reagents and staining time. It's still not the quality that we are looking for. I have had culligan techs out several times to see if it could be something with the water. We can run 100 slides the same day, same reagents and protocol and the H&E color is so inconsistent. I would appreciate any suggestions in this matter. Cherie Chapman, BS, HT, HTL (ASCP) Associate Director of Dermatopathology Laboratory University of Missouri Department of Dermatology University Physicians Medical Building Phone: (573) 884-0123 Fax: (573) 884-0834 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cls71877 <@t> gmail.com Wed Dec 4 12:25:04 2013 From: cls71877 <@t> gmail.com (Cristi Stephenson) Date: Wed Dec 4 12:25:14 2013 Subject: [Histonet] (no subject) In-Reply-To: <3AD061FE740D464FAC7BF6B5CFB75707A95A6CE0@SMCMAIL01.somerset-healthcare.com> References: <3AD061FE740D464FAC7BF6B5CFB75707A95A6CE0@SMCMAIL01.somerset-healthcare.com> Message-ID: I second Toni's suggestion. Definitely sounds like the paraffin is still present on the lower sections of the slide. On Wed, Dec 4, 2013 at 10:17 AM, Rathborne, Toni < trathborne@somerset-healthcare.com> wrote: > Could it be the heating/deparaffinization process? If the upper sections > are staining more evenly, then maybe they are free from residual paraffin. > Try extending the time in the ovens and/or xylene. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Chapman, Cherie J. > Sent: Wednesday, December 04, 2013 1:12 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] (no subject) > > Hello all, > > I am looking for suggestions on issues with our H&E stain. > > I supervised a Veterinary Diagnostic lab for over 27 years and produced > top quality sections, H&E's, special stains and IHC on a variety of > different species in our lab. > I am currently working in a Dermatopathology Lab and I am finding > inconsistent staining with our H&E's. Working with just skin is a challenge > all on its own. We have made changes to our staining protocol and just not > happy with the end product. > What we are observing is inconsistent staining on levels on the same > slide. The top section seems to stain more evenly than the middle and > bottom sections. I can actually see three different shades of color. The > specimens are ribboned sections so I know it is not from thick and thin > sections. We have looked at our processing times, microwave vs. oven > times, staining reagents, different brands of hematoxylin and eosin, > adjustments on staining times, tap water compared to distilled water. > > Our main processor is the Thermo Scientific STP-420 and our back up is the > Sakura VIP V processor. I have been working with Thermo technical > support thinking it might be a processing issue. We have a Leica ST5020 > Multstainer/CV5030 Robotic Cover slipper we have made several changes that > the technical teams has suggested to the reagents and staining time. It's > still not the quality that we are looking for. > I have had culligan techs out several times to see if it could be > something with the water. > > We can run 100 slides the same day, same reagents and protocol and the H&E > color is so inconsistent. > I would appreciate any suggestions in this matter. > > > Cherie Chapman, BS, HT, HTL (ASCP) > Associate Director of Dermatopathology Laboratory University of Missouri > Department of Dermatology University Physicians Medical Building > Phone: (573) 884-0123 > Fax: (573) 884-0834 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From ttruscot <@t> vetmed.wsu.edu Wed Dec 4 12:40:07 2013 From: ttruscot <@t> vetmed.wsu.edu (Truscott, Tom) Date: Wed Dec 4 12:40:12 2013 Subject: [Histonet] (no subject) In-Reply-To: <3AD061FE740D464FAC7BF6B5CFB75707A95A6CE0@SMCMAIL01.somerset-healthcare.com> References: <3AD061FE740D464FAC7BF6B5CFB75707A95A6CE0@SMCMAIL01.somerset-healthcare.com> Message-ID: <9EF5279EBDFE6E4FB6605E8F183A00276A7CC3B8@CVM76.vetmed.wsu.edu> Hi Cherie, Since the top section is consistently better, then the possibilities lie in what happens after they are on the slide. As Toni recommends slides that are heated or deparaffinized vertically may have left over paraffin on the lower regions.Maybe you could also try depar with them up on their long edges. Staining slides vertically might also give this appearance if reagent times and rinsing are too short. Staining slides flat would point to slides not level or reagents not evenly dispersed on the slide. I would not rule out thick-thin on ribbons but that is probably not the case since the good section is always at the top. Good luck, Tom T -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Wednesday, December 04, 2013 10:17 AM To: 'Chapman, Cherie J.'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] (no subject) Could it be the heating/deparaffinization process? If the upper sections are staining more evenly, then maybe they are free from residual paraffin. Try extending the time in the ovens and/or xylene. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Chapman, Cherie J. Sent: Wednesday, December 04, 2013 1:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Hello all, I am looking for suggestions on issues with our H&E stain. I supervised a Veterinary Diagnostic lab for over 27 years and produced top quality sections, H&E's, special stains and IHC on a variety of different species in our lab. I am currently working in a Dermatopathology Lab and I am finding inconsistent staining with our H&E's. Working with just skin is a challenge all on its own. We have made changes to our staining protocol and just not happy with the end product. What we are observing is inconsistent staining on levels on the same slide. The top section seems to stain more evenly than the middle and bottom sections. I can actually see three different shades of color. The specimens are ribboned sections so I know it is not from thick and thin sections. We have looked at our processing times, microwave vs. oven times, staining reagents, different brands of hematoxylin and eosin, adjustments on staining times, tap water compared to distilled water. Our main processor is the Thermo Scientific STP-420 and our back up is the Sakura VIP V processor. I have been working with Thermo technical support thinking it might be a processing issue. We have a Leica ST5020 Multstainer/CV5030 Robotic Cover slipper we have made several changes that the technical teams has suggested to the reagents and staining time. It's still not the quality that we are looking for. I have had culligan techs out several times to see if it could be something with the water. We can run 100 slides the same day, same reagents and protocol and the H&E color is so inconsistent. I would appreciate any suggestions in this matter. Cherie Chapman, BS, HT, HTL (ASCP) Associate Director of Dermatopathology Laboratory University of Missouri Department of Dermatology University Physicians Medical Building Phone: (573) 884-0123 Fax: (573) 884-0834 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Wed Dec 4 12:57:35 2013 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Wed Dec 4 12:57:40 2013 Subject: [Histonet] On-slide IHC control workflow? In-Reply-To: <761E2B5697F795489C8710BCC72141FF0B56E8@ex07.net.ucsf.edu> References: <761E2B5697F795489C8710BCC72141FF0B56E8@ex07.net.ucsf.edu> Message-ID: Tim Of course not every method works everywhere....here is what I do for myself. I use a mix of MTB blocks with some single tissue when scarcity of tissue makes this sensible. The MTB blocks are based on the IHC panels Make own MTB blocks , feel too expensive to buy for me right now, but might make sense for others. I don't have a micro array tool right now so I do my own improvisingAssign control cutting as a rotated duty. Keep a log of the source block and the number of control slides prepared, (after awhile this helps in cutting down on pulling incorrect controls too)Breast markers, other finicky- refrigerate, (Her 2 - date and use within 6 weeks per CAP) other controls at room temperature in labeled slide boxes. I keep the current control with the slides in the box until it is exhaustedCut more slides for MTB blocks, less of not often requested or single tissue controls- could set stock or par numbers, but haven't needed to do this yetI have controls in the SOP and a table in excel of the current controls, but you can also write it on a copy of the request form beside the name of the AB and tape it up if it changes oftenPeople should be able to pull the correct controls from that, and they are required to check the stain under the 'scope anyhow, so hopefully that would assist in keeping the wrong control from going out I am kind of picky about the record-keeping, but in the end I feel it saves more time than not. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: Timothy.Morken@ucsfmedctr.org > To: histonet@lists.utsouthwestern.edu > Date: Wed, 4 Dec 2013 17:45:43 +0000 > Subject: [Histonet] On-slide IHC control workflow? > > We are planning our move to using on-slide controls for IHC and I'm wondering how other labs handle the workflow and logistics of matching controls to stain orders. > > We plan to use a TMA for 80% of our orders. So far we have one TMA that covers most Ab's but the number will probably will be expanded (neuropath-specific, Hempath specific, etc). > > For those who do this now, how do you handle these tasks: > > > * Do you use TMA or single-tissue controls? Or a mix? > > * > > * Cutting all the controls - one or more techs? Part of normal work? Outsource? (especially TMA cutting - inhouse or outsource?) > > > * How do you store the controls? (We plan to cut nearly just-in-time, maybe two days from use). > > > * How do you distribute to the cutters? > > > * How do you Indicate to the cutting techs which control slide to use for a particular stain? (we use over 200 antibodies so need to make as easy as possible without memorization) > > > * How do you prevent the wrong control slide from being used? > > > Anything else we should consider? > > Thanks for any help!! > > Tim Morken > Supervisor, Electron Microscopy and Neuromuscular Special Studies > UC San Francisco Medical Center > Box 1656 > 505 Parnassus Ave > San Francisco, CA 94143 > USA > > 415.353.1266 (office) > tim.morken@ucsfmedctr.org > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LSebree <@t> uwhealth.org Wed Dec 4 12:59:09 2013 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Wed Dec 4 12:59:14 2013 Subject: [Histonet] RE: On-slide IHC control workflow? In-Reply-To: <761E2B5697F795489C8710BCC72141FF0B56E8@ex07.net.ucsf.edu> References: <761E2B5697F795489C8710BCC72141FF0B56E8@ex07.net.ucsf.edu> Message-ID: <77DD817201982748BC67D7960F2F76AF072EC9@UWHC-MBX12.uwhis.hosp.wisc.edu> This is what we've done when we recently moved to control tissue on every slide for IHC as well as Special Stains. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Wednesday, December 04, 2013 11:46 AM To: Histonet Subject: [Histonet] On-slide IHC control workflow? We are planning our move to using on-slide controls for IHC and I'm wondering how other labs handle the workflow and logistics of matching controls to stain orders. We plan to use a TMA for 80% of our orders. So far we have one TMA that covers most Ab's but the number will probably will be expanded (neuropath-specific, Hempath specific, etc). For those who do this now, how do you handle these tasks: * Do you use TMA or single-tissue controls? Or a mix? We use single tissues for most and have decreased the number of different tissues being used as controls to a more generic tissue that will cover a wider range of antibodies. * Cutting all the controls - one or more techs? Part of normal work? Outsource? (especially TMA cutting - inhouse or outsource?) Cutting controls is a component of several of the histologists' rotations so every week at least 2 people have this task as part of their rotation. * How do you store the controls? (We plan to cut nearly just-in-time, maybe two days from use). Most are stored at RT; some are stored at - 20 degrees C for those antigens that may lose reactivity at RT * How do you distribute to the cutters? The IHC lab prints control slides and pulls the corresponding blocks to have at the ready for those people on the rotations that include cutting controls...they come pick the slides & blocks up when they're ready to cut * How do you Indicate to the cutting techs which control slide to use for a particular stain? (we use over 200 antibodies so need to make as easy as possible without memorization) We have a master list of our antibodies posted where IHC slides are organized, that indicates which control tissue to use for each antibody. * How do you prevent the wrong control slide from being used? People learn....if a mistake is made, that person is "written up" so the importance of paying attention to detail is reinforced. Anything else we should consider? We cut large amounts, i.e. 200 tonsil controls, at a time rather than a couple days before the need for control slides. These are kept in slide files in the area of IHC slide organization where the barcode labels are printed for use on our IHC stainers. The control slides are then labeled along with blank slides for the negative controls and taken to microtomy work stations ; works well for us. Thanks for any help!! Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center Box 1656 505 Parnassus Ave San Francisco, CA 94143 USA 415.353.1266 (office) tim.morken@ucsfmedctr.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From chapmanc <@t> health.missouri.edu Wed Dec 4 13:44:20 2013 From: chapmanc <@t> health.missouri.edu (Chapman, Cherie J.) Date: Wed Dec 4 13:44:30 2013 Subject: [Histonet] (no subject) Message-ID: Thank you for all the suggestions. Nothing more frustrating when you can't point your finger to the exact problem. Thanks again, Cherie Chapman, BS, HT, HTL (ASCP) Associate Director of Dermatopathology Laboratory University of Missouri Department of Dermatology University Physicians Medical Building Phone: (573) 884-0123 Fax: (573) 884-0834 From tkngflght <@t> yahoo.com Wed Dec 4 14:18:43 2013 From: tkngflght <@t> yahoo.com (Cheryl) Date: Wed Dec 4 14:18:47 2013 Subject: [Histonet] Helicobacter helmani -- help? Message-ID: <1386188323.54812.YahooMailNeo@web161906.mail.bf1.yahoo.com> Hi Guys- Does the routine Helicobacter pylori IHC staining generally stain the whole spectrum of helicos? We had a clinician ask specifically about the helmani and we're waiting for a response from the vendor but now I'm curious, too! Thanks in advance and Wednesday-- Cheryl Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab by helping one GREAT?Tech at a time.? 281.852.9457?Office 800.756.3309?Phone & Fax? admin@fullstaff.org Sign up for the FREE?newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please?include your name and specialty in the body of the email. From Vickroy.Jim <@t> mhsil.com Wed Dec 4 14:50:51 2013 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Wed Dec 4 14:50:59 2013 Subject: [Histonet] CAP survey Question Message-ID: For a long time I have had our IHC techs print out the run logs from each IHC run on the Benchmark Ultras. The techs then check the slides to make sure the positive and negative controls have worked properly before the slides are sent to the individual pathologists. The pathologists are also supposed to check the controls before looking at the patient slides. Lately in the interest of reducing turnaround time I have been asked why we run the log reports and have a tech look over the controls before they send them to the pathologists since the pathologist will also evaluate the controls. I have been doing this because I wanted the documentation that someone reviewed the controls each time an IHC stain was done. I believe if the pathologists would document someplace that the control slides were reviewed before the patient slides were viewed then I could eliminate the techs looking over the controls also. Problem is how are others documenting that the controls are reviewed? Is this done by the techs, the pathologists, or both? We of course have also used this data for quality assurance of our stains. Thanks for your help. Jim James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From leila.etemadi <@t> med.lu.se Wed Dec 4 15:10:42 2013 From: leila.etemadi <@t> med.lu.se (Leila Etemadi) Date: Wed Dec 4 15:10:51 2013 Subject: [Histonet] rat's hind paw skin In-Reply-To: References: <17C4C4E7-B1A8-4D51-95AC-218FE8737291@med.lu.se> Message-ID: <223AA95F-175F-4A1C-AC92-DB9488069A61@med.lu.se> Hi, First I would like to say thanks a lot to you and also Carl who gave me some helpful tips. Then I would like to ask if you have any practical tips when I want to pick up the skin from perfused animal. I need those special tricks which are not normally mentioned in the articles!, I am going to collect them nest week, kind of stressed since I don?t want to lose the tissues because of clumsy handling!, I you have any tips or link which can help me??!? Thanks a lottttt? Cheers, Leila On 03 Dec 2013, at 01:27, Barry Rittman wrote: > Hi Leila > As no one seems to have responded to your email I will add a few items. > I used to do some work on wound healing on mouse footpads so circumstances > are the same. > If you are looking for melanin routine fixation and processing should be > finem, melanin will remain. > Only thing is that the epidermis is tough and there is not much dermis in > this region. > Need to make sure that you are careful in cutting sections or the epidermis > will separate. > Hope this helps > if you need any more details please email me. > Barry > > > > On Mon, Dec 2, 2013 at 3:44 AM, Leila Etemadi wrote: > >> Hello every body, >> >> First I would like to thank you all for your great feedbacks and attention >> which is such a big improvement in my work, Thank you all! >> >> Then, I was wonder if any one has experience on this tissue: rat hind paw >> skin ( which is hairless). >> >> Any inputs will be appreciated, whether is about any specific source where >> I can find technical information about fixation procedure..., or about >> sectioning,staining procedure? >> >> I am looking for melanin pigments and have no clue if it is possible to >> see it there or not?!? >> >> Thanks in advance, >> >> Cheers >> >> Leila >> >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Richard.Cartun <@t> hhchealth.org Wed Dec 4 15:31:17 2013 From: Richard.Cartun <@t> hhchealth.org (Cartun, Richard) Date: Wed Dec 4 15:31:22 2013 Subject: [Histonet] Helicobacter helmani -- help? In-Reply-To: <1386188323.54812.YahooMailNeo@web161906.mail.bf1.yahoo.com> References: <1386188323.54812.YahooMailNeo@web161906.mail.bf1.yahoo.com> Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E01897757@HHCEXCHMB05.hhcsystem.org> The polyclonal antibody from Dako labels H. helmennia in addition to H. pylori. Several of the commercially-available mAbs to H. pylori that I have evaluated do not label H. helmennia. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax richard.cartun@hhchealth.org ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Cheryl [tkngflght@yahoo.com] Sent: Wednesday, December 04, 2013 3:18 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Helicobacter helmani -- help? Hi Guys- Does the routine Helicobacter pylori IHC staining generally stain the whole spectrum of helicos? We had a clinician ask specifically about the helmani and we're waiting for a response from the vendor but now I'm curious, too! Thanks in advance and Wednesday-- Cheryl Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab by helping one GREAT Tech at a time. 281.852.9457 Office 800.756.3309 Phone & Fax admin@fullstaff.org Sign up for the FREE newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please include your name and specialty in the body of the email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From LSebree <@t> uwhealth.org Wed Dec 4 15:33:56 2013 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Wed Dec 4 15:34:00 2013 Subject: [Histonet] RE: CAP survey Question In-Reply-To: References: Message-ID: <77DD817201982748BC67D7960F2F76AF072FB9@UWHC-MBX12.uwhis.hosp.wisc.edu> We also have Ultras Jim. We don't print out the run logs. IHC personnel review the controls before they go to a pathologist in order to catch any problems. The pathologists are supposed to review the controls associated with each case they sign out. Our pathology report has a statement included that the negative and positive controls have stained appropriately. By signing off on each case, the pathologist is attesting to the fact that he has indeed reviewed the controls...we do not police them. So far, this works for us. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Wednesday, December 04, 2013 2:51 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP survey Question For a long time I have had our IHC techs print out the run logs from each IHC run on the Benchmark Ultras. The techs then check the slides to make sure the positive and negative controls have worked properly before the slides are sent to the individual pathologists. The pathologists are also supposed to check the controls before looking at the patient slides. Lately in the interest of reducing turnaround time I have been asked why we run the log reports and have a tech look over the controls before they send them to the pathologists since the pathologist will also evaluate the controls. I have been doing this because I wanted the documentation that someone reviewed the controls each time an IHC stain was done. I believe if the pathologists would document someplace that the control slides were reviewed before the patient slides were viewed then I could eliminate the techs looking over the controls also. Problem is how are others documenting that the controls are reviewed? Is this done by the techs, the pathologists, or both? We of course have also used this data for quality assurance of our stains. Thanks for your help. Jim James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amurvosh <@t> advancederm.net Wed Dec 4 15:36:57 2013 From: amurvosh <@t> advancederm.net (Anne Murvosh) Date: Wed Dec 4 15:37:04 2013 Subject: [Histonet] Helicobacter helmani -- help? In-Reply-To: <1386188323.54812.YahooMailNeo@web161906.mail.bf1.yahoo.com> References: <1386188323.54812.YahooMailNeo@web161906.mail.bf1.yahoo.com> Message-ID: <4AD6A4E531E8C943A730559B6B81DF07C8A027@dc.Advancederm.net> We had same problem at my old job. I don't think they have come up with a separate IHc so our doctor would order an alcian yellow with ihc on children under age of 12 I think but we gave this up cause it's so rare. If you look up on Google under h. Heilmannii, I believe its spelled, it has a lot of papers written on it that might help. Anne -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl Sent: Wednesday, December 04, 2013 12:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Helicobacter helmani -- help? Hi Guys- Does the routine Helicobacter pylori IHC staining generally stain the whole spectrum of helicos? We had a clinician ask specifically about the helmani and we're waiting for a response from the vendor but now I'm curious, too! Thanks in advance and Wednesday-- Cheryl Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab by helping one GREAT?Tech at a time.? 281.852.9457?Office 800.756.3309?Phone & Fax? admin@fullstaff.org Sign up for the FREE?newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please?include your name and specialty in the body of the email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cheastys <@t> svm.vetmed.wisc.edu Wed Dec 4 16:10:21 2013 From: cheastys <@t> svm.vetmed.wisc.edu (Sandra Cheasty) Date: Wed Dec 4 16:10:19 2013 Subject: [Histonet] IHC Buffer (wash) Message-ID: Hello everyone, Got your Christmas shopping done? (Didn't think so...) Anyway, I'm looking for an IHC PBS/Tween buffer wash concentrate that is comparable to the very expensive Lab Vision PBS/Tween buffer wash. I see some at VWR and FISHER (no shipping charges for our lab) that are 0.5% and 1% Tween 20 washing buffer in PBS. Which percentage is best for IHC on a Lab Vision stainer using a polymer detection kit? As usual, your input is appreciated. Sandy P.S. I know I can make up my own from scratch, just not interested in doing that at the moment... Sandra Cheasty Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine 608 263-1680 (Office) 608 263-1055 (Lab) From tony.henwood <@t> health.nsw.gov.au Wed Dec 4 17:40:10 2013 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Wed Dec 4 17:40:33 2013 Subject: [Histonet] RE: Yahoo link NO PHI In-Reply-To: <38667E7FB77ECD4E91BFAEB8D986386325011F302B@LRGHEXVS1.practice.lrgh.org> References: <63f258ee13dc423ab0531cb811d5d71e@BY2PR07MB469.namprd07.prod.outlook.com><761E2B5697F795489C8710BCC72141FF0B52C9@ex07.net.ucsf.edu><1386088821.21321.YahooMailNeo@web5704.biz.mail.ne1.yahoo.com><9BF995BC0E47744E9673A41486E24EE25845D838DD@MERCERMAIL.MercerU.local>, <761E2B5697F795489C8710BCC72141FF0B534C@ex07.net.ucsf.edu> <25A4DE08332B19499904459F00AAACB719DA7CAF24@EVS1.archildrens.org> <9BF995BC0E47744E9673A41486E24EE25845D83E22@MERCERMAIL.MercerU.local> <38667E7FB77ECD4E91BFAEB8D986386325011F302B@LRGHEXVS1.practice.lrgh.org> Message-ID: <6D6BD1DE8A5571489398B392A38A71579D47798B@xmdb04.nch.kids> Oh Poop.... I mean Damm Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas Sent: Thursday, 5 December 2013 4:17 AM To: Shirley A. Powell; Weems, Joyce K.; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Yahoo link NO PHI My current lab the air exchange rate is 58/hr. The good news: you do not smell fumes of any kind. The bad news: we are negative pressure and the unvented bathrooms are just outside our lab. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shirley A. Powell Sent: Wednesday, December 04, 2013 11:42 AM To: Weems, Joyce K.; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Yahoo link I had the same experience, the clinical lab techs asked the supervisor to keep our door closed so they would not have to smell the fumes, just lock us up in it. I was considering calling EPA in to check it out. Thank goodness those days are gone. Use those fume hoods and all the other ppe you can get. :) Shirley Powell -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Wednesday, December 04, 2013 10:57 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Yahoo link I worked in a non-ventilated lab once - back in the 70s - just before the xylene study was released. I knew something was wrong and I knew it was xylene and finally was able to get an exhaust put in the window . I could taste it, smell it, and seemed to cough it up!! And my brain was fuzzier than my usual fuzz! The lab director's office was next to my lab and she moved, because she said she couldn't stand to be next to me! So thankful for good regs now and labs since then that have been very well ventilated. We have come a long way, baby!! Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Wednesday, December 04, 2013 9:52 AM To: 'Lee & Peggy Wenk'; Elizabeth Cameron; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Yahoo link I remember participating in the health study in the 80's. Xylene and Formaldehyde levels are monitored in all labs. If our hospitals/research centers would allow us to forward that information on to someone who could compile data it would be a starting place for a health study. I strongly believe this profession can be danger to health. I can name at least 10 histotechs who have died from cancer. Yes, it may have been when they were older but I feel certain it was from all the chemical exposure in histology before safety became a priority. I know others will remember no ventilation, smoking and eating in the lab, no real requirements for gloves, etc. There were also no MSDS information available and we were exposed through lack of knowledge. I believe the lab is much safer today with all of the safety precautions we take and the knowledge we have on chemicals/stains and their toxicity. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk Sent: Wednesday, December 04, 2013 8:24 AM To: Elizabeth Cameron; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Yahoo link Couple of studies that I know of. One was sponsored by NSH in the mid-1980's. KH Kilburn came to several NSH Symposiums, and did different tests on people who volunteered to participate. Published findings in the late 1980's that said that histotechs had lower pulmonary function than average population, and decreased memory, equilibrium and dexterity than the general population. In Letters to the Editor, people pointed out statistical flaws (low numbers of participants, for example). I also feel there were flaws, such as testing people after traveling over time zones, who were up late at the parties, and had possibly been drinking the night before. There was no way to measure how much exposure to formaldehyde or xylene people were really exposed to. I didn't participate, but if I though the amount I was being exposed to was medium, someone else being exposed to the same amount might have said low amount and someone else could have said high amount. And the studies would say therefore the low pulmonary exposure was due to histotechs being exposed to formaldehyde. But who could say it was due to that chemical, and not due another chemical, or due to the fact that at the same time, people were smoking in the lab I was working in, which was a small space. Another study somewhat relates - S Khattak in 1999 wrote one on pregnancy outcomes following gestational exposure to organic solvents. They interview women who were pregnant and working with organic solvent, so painters for example, so not histotechs only. They compared them to women of same age, same number of children, similar profession not exposed to organic solvents. They found that if the exposed women were having symptoms (breathing problems, rashes), they had a higher percentage of miscarriages and babies with deformities. If there were no symptoms, they had the same, and even lower, percentages than comparable women giving birth who were not exposed. What histology needs is something like the nursing organization in the US has been doing for over 40 years. Everyone who is a member of the nursing society is sent a survey (I think every year), and asked to have physicals information released from their doctors to the organization (It's voluntary to participate). But they have 40+ years of data from hundred of thousands of women, of all ages. They can mine a wealth of medical data from this. NSH (and ASCP) may not have enough histotechs in their organization to pay for the type of survey needed, to continue on for decades. We would need supervisors and bench techs to participate for decades. And probably have to mail them several formaldehyde and xylene monitors every year, or every couple of years, to collect real numbers of how much people were exposed to. And the surveys were pages and pages long (my mother was a nurse who participated from the beginning, so I've seen them), and asked lots of questions about health and diet and smoking and personal issues, in addition to questions about what types of chemical we work with an how much and how often. And how do we measure ventilation in all these places of work? I hope someone comes up with some studies that can help us figure out if histotechs are being exposed to enough chemicals that could be causing these different diseases in humans. There are animal studies, but not human. And remember, people in general, including histotechs, are living longer. If we live longer, we are more likely to have chronic diseases like diabetes, COPD, and cancer. We need to know which cancers are caused by which levels of formaldehyde or organic solvents, vs., say, breast cancer and prostate cancer just because we are women or men. Peggy A. Wenk, HTL(ASCP)SLS -----Original Message----- From: Elizabeth Cameron Sent: Wednesday, December 04, 2013 8:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Yahoo link I know the potential for damage to your health is huge in histology, but are there any studies out there that indicate histotechs are less healthy than the average person? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Tuesday, December 03, 2013 7:06 PM To: Histonet Subject: [Histonet] RE: Yahoo link Old Histologists never die, they're just well fixed... Claire ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Morken, Timothy [Timothy.Morken@ucsfmedctr.org] Sent: Tuesday, December 03, 2013 11:22 AM To: 'Shirley A. Powell'; Histonet Subject: [Histonet] RE: Yahoo link Well, Shirley, you are actually an Angel, so nothing will ever stop you!! (from an old Georgia Society hand). Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shirley A. Powell Sent: Tuesday, December 03, 2013 9:18 AM To: Histonet Subject: [Histonet] Yahoo link When I entered the profession I was told the average life expectancy of a histotechs was 20 years from hiring. That scared me but I was already hooked. I have been doing this 51 years, so maybe good laboratory practices can help, in spite of bad ventilation and all those other dangers mentioned. Shirley Powell Antique Histotech -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Tuesday, December 03, 2013 11:40 AM To: Morken, Timothy; Histonet Subject: Re: [Histonet] RE: Yahoo link Just what I was thinking. We will never get enough new students now! With proper training and equipment, we do the job safely! And you can drown in an inch of water. Is that in the MSDS! Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 5830 N Blue Lake Dr. Please note new address! Norman, OK 73069 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ________________________________ From: "Morken, Timothy" To: 'Mike Tighe' ; "histonet@lists.utsouthwestern.edu (histonet@lists.utsouthwestern.edu)" Sent: Tuesday, December 3, 2013 10:24 AM Subject: [Histonet] RE: Yahoo link Great. Just what we need. Even so, we in the business can take these lists with a grain of K4[Fe(CN) 6] * 3H 2 O since we know that suitable precautions preclude most of the danger. For instance, I'll have to say that the histo lab here is wonderful in that it has such good ventilation (ie., extraction) that there is none of the usual chemical smell - no xylene, alcohol, specials chemicals that often assaults the senses in histology. Vendors that come here are amazed. Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Tighe Sent: Tuesday, December 03, 2013 6:31 AM To: histonet@lists.utsouthwestern.edu (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Yahoo link Anybody wonder who has the most harzardous job to your health? We're Number One!!! http://finance.yahoo.com/news/the-15-jobs-that-are-most-damaging-to-your-health-155706120.html Mike _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as possible. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** This message is confidential, intended only for the named recipient(s) and may contain information that is privileged, attorney work product, highly confidential, or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender by reply electronic mail, delete this electronic mail from your computer, and destroy any copies in any form immediately. Receipt by anyone other than the named recipient(s) is not a waiver of any attorney-client, work product, or other applicable privilege. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From tony.henwood <@t> health.nsw.gov.au Wed Dec 4 17:42:24 2013 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Wed Dec 4 17:42:37 2013 Subject: [Histonet] RE: Rush bx schedule? In-Reply-To: <761E2B5697F795489C8710BCC72141FF0B56B4@ex07.net.ucsf.edu> References: <761E2B5697F795489C8710BCC72141FF0B56B4@ex07.net.ucsf.edu> Message-ID: <6D6BD1DE8A5571489398B392A38A71579D47799C@xmdb04.nch.kids> Hi Tim, Try fixing for 45 min at 50oC. I think it possible that the tissue is not adequately fixed. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Thursday, 5 December 2013 4:31 AM To: Histonet Subject: [Histonet] Rush bx schedule? For tissue, what step most contributes to subsequent swelling of tissue when the block is soaked? I'm thinking the 100% ETOH, xylene clearing and paraffin infiltration all contribute, but at what percentage? Here is the current schedule, which apparently was meant to mimic a microwave schedule that was used before the MW quit working. The tissue - kidney and liver bx for the most part, up to 10 per day, is coming out soft and swells a bit when soaked. So sectioning is difficult. We need to have a rapid schedule but need good sections. Obviously we are pushing the limits on this so need a bit of leeway for variation in tissue So, I'm considering which of these steps is the most critical to lengthen. This is on a VIP5 but we can also use a peloris for this processing Formalin 2 min, 50degC (this is not necessary at all, IMHO) 80% ETOH 5 min, 50degC 95%ETOH 5 min, 50degC 100% ETOH 8 min, 50degC Xylene, 9 min 50degC Paraffin, 4 min, Paraffin, 5 min Thanks for your insight! Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center Box 1656 505 Parnassus Ave San Francisco, CA 94143 USA 415.353.1266 (office) tim.morken@ucsfmedctr.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From tony.henwood <@t> health.nsw.gov.au Wed Dec 4 18:03:05 2013 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Wed Dec 4 18:03:19 2013 Subject: [Histonet] RE: On-slide IHC control workflow? In-Reply-To: <761E2B5697F795489C8710BCC72141FF0B56E8@ex07.net.ucsf.edu> References: <761E2B5697F795489C8710BCC72141FF0B56E8@ex07.net.ucsf.edu> Message-ID: <6D6BD1DE8A5571489398B392A38A71579D4779E9@xmdb04.nch.kids> Unfortunately some controls cannot be confidently used in a TMA eg CD15 - where often Hodgkin's cells are sparse in the block, or CMV or adenovirus - again same reason - sparse positive cells. We sometimes use composite blocks, eg skin and lymph node for S100 and CD1a - (dendritic cells) or (in my past) Prostate adenocarcinoma and salivary gland for prostate specific antigen. We tend to cut our controls and place them near the label end of the slide. We do not heat them but dry them (10minutesbefore placing in a closed labelled box ready for use. The box is labelled with the antigens the control slides are used for. There might be issues with aged pre-cut slides but we have not noticed ant deterioration with the antibodies we use. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Thursday, 5 December 2013 4:46 AM To: Histonet Subject: [Histonet] On-slide IHC control workflow? We are planning our move to using on-slide controls for IHC and I'm wondering how other labs handle the workflow and logistics of matching controls to stain orders. We plan to use a TMA for 80% of our orders. So far we have one TMA that covers most Ab's but the number will probably will be expanded (neuropath-specific, Hempath specific, etc). For those who do this now, how do you handle these tasks: * Do you use TMA or single-tissue controls? Or a mix? * * Cutting all the controls - one or more techs? Part of normal work? Outsource? (especially TMA cutting - inhouse or outsource?) * How do you store the controls? (We plan to cut nearly just-in-time, maybe two days from use). * How do you distribute to the cutters? * How do you Indicate to the cutting techs which control slide to use for a particular stain? (we use over 200 antibodies so need to make as easy as possible without memorization) * How do you prevent the wrong control slide from being used? Anything else we should consider? Thanks for any help!! Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center Box 1656 505 Parnassus Ave San Francisco, CA 94143 USA 415.353.1266 (office) tim.morken@ucsfmedctr.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From carl.hobbs <@t> kcl.ac.uk Wed Dec 4 22:56:25 2013 From: carl.hobbs <@t> kcl.ac.uk (Hobbs, Carl) Date: Wed Dec 4 22:56:33 2013 Subject: [Histonet] rat's hind paw skin Message-ID: <89c94ddc9db5459faf42ca69f3e58747@DBXPR03MB413.eurprd03.prod.outlook.com> If they are perfused, you don't need to worry about stopping them from curling ( they will be fixed/rigid). Get as large a piece as possible: preferably two pieces. (The second piece can then be further fixed for 2hrs before placing into 30% sucrose until the specimen sinks, then snap-freezing. If you don't have a cryostat, forget this.) The piece for Pwax: place in a cassette that you know has holes too small for the specimen to fall thro. I use those plastic mesh inserts: helps to keep tissue flat and "safe". They fit nicely into std plastic cassettes. If your current processing protocol is good for your specimens, it will be fine for rat footpad skin. ( if you are going to do HIER....you will have to use charged/coated slides, of course.) You will lose some integrity of the dermis ( collagen will be disrupted), unless you play around with sub-boiling temps. Thanks and.....good luck. Carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge Kings College London London SE1 1UL 020 7848 6813 From Michael.LaFriniere <@t> ccplab.com Thu Dec 5 07:18:25 2013 From: Michael.LaFriniere <@t> ccplab.com (Michael LaFriniere) Date: Thu Dec 5 07:18:33 2013 Subject: [Histonet] RE: Stainer/coverslipper question In-Reply-To: <12ECD7346266D74691EC2BFC75285E452F365D17@BFL323E10.pathmdlabs.local> References: <12ECD7346266D74691EC2BFC75285E452F365D17@BFL323E10.pathmdlabs.local> Message-ID: <4A2A16B9707CE04E9CB6C82DC18C1D2963E49E@AHCMSASEXCH03.my.ahc.local> A few months ago I implemented the Lieca stainer with coverslipper, great pricing on the unit! It works well, the coverslipper can be a little touchy at first but once you break it in, it works well...we went to imagining some slides and wanted glass coverslips. On another note, I just implemented and validated the new Dako Omnis immuno unit...amazing!! I will be happy to answer any questions privately so I don't seem to be advertising for a company on histonet.... Michael Michael R. LaFriniere, HT (ASCP) Executive Director Capital Choice Pathology Laboratory 12041 Bournefield Way, Suite A * Silver Spring, MD 20904 P: 240.471.3427 * F: 240.471.3401 * Cell 410-940-8844 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Tuesday, December 03, 2013 4:10 PM To: Connolly, Brett M; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Stainer/coverslipper question Hi Brett, I have used the Prisma Stainer with the film coverslipper connected, and loved them both. The Prisma stainer can hold 3 racks of 20 slides, so it is very nice for high volume. Our current stainer only holds one rack of 20, and slides can get backed up. The coverslipper is extremely fast and very trouble-free. I have heard that Sakura's glass coverslipper is good. We currently use the ClearVue glass coverslipper from Thermo, and it works well. Laurie Colbert, HT (ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Connolly, Brett M Sent: Tuesday, December 03, 2013 12:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Stainer/coverslipper question Hello all, We are looking at options for automated stainer/glass coverslipper workstations. I browsed the Histonet archives and see many varied love/hate responses from over the years about reliability, need to babysit, bubbles etc. and I am wondering if things have improved as many past inquiries were fairly old. >From the archives, it seems that Lieca ST5020/CV5030 and the Sakura (Tissue-Tek) Prisma/Glas g2 are the main players. Our H&E workload is highly varied, but we want to also use the workstation for dehydrating and coverslipping our immuno slides that come off our IHC stainer in H2O. I would appreciate your recommendations/experiences. Thanks much, Brett Brett M. Connolly, Ph.D. Principal Scientist, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TGoins <@t> mt.gov Thu Dec 5 09:28:28 2013 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Thu Dec 5 09:28:46 2013 Subject: [Histonet] RE: IHC Buffer (wash) In-Reply-To: References: Message-ID: Try ScyTek - The ready-made price is nearly as cheap as I can make it myself. http://www.scytek.com/default.asp Tresa Tresa Goins Histopathology Section Supervisor Montana Veterinary Diagnostic Lab -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sandra Cheasty Sent: Wednesday, December 04, 2013 3:10 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC Buffer (wash) Hello everyone, Got your Christmas shopping done? (Didn't think so...) Anyway, I'm looking for an IHC PBS/Tween buffer wash concentrate that is comparable to the very expensive Lab Vision PBS/Tween buffer wash. I see some at VWR and FISHER (no shipping charges for our lab) that are 0.5% and 1% Tween 20 washing buffer in PBS. Which percentage is best for IHC on a Lab Vision stainer using a polymer detection kit? As usual, your input is appreciated. Sandy P.S. I know I can make up my own from scratch, just not interested in doing that at the moment... Sandra Cheasty Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine 608 263-1680 (Office) 608 263-1055 (Lab) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SHUNTER <@t> beaumont.edu Thu Dec 5 09:46:08 2013 From: SHUNTER <@t> beaumont.edu (Sue Hunter) Date: Thu Dec 5 09:46:13 2013 Subject: [Histonet] RE: CAP survey Question In-Reply-To: <77DD817201982748BC67D7960F2F76AF072FB9@UWHC-MBX12.uwhis.hosp.wisc.edu> References: <77DD817201982748BC67D7960F2F76AF072FB9@UWHC-MBX12.uwhis.hosp.wisc.edu> Message-ID: We do the same process as Linda does here at Beaumont. Sue Sue Hunter, Supervisor Advanced Diagnostics Beaumont Health System Royal Oak MI 248-898-5146 shunter@beaumont.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A Sent: Wednesday, December 04, 2013 4:34 PM To: 'Vickroy, Jim'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: CAP survey Question We also have Ultras Jim. We don't print out the run logs. IHC personnel review the controls before they go to a pathologist in order to catch any problems. The pathologists are supposed to review the controls associated with each case they sign out. Our pathology report has a statement included that the negative and positive controls have stained appropriately. By signing off on each case, the pathologist is attesting to the fact that he has indeed reviewed the controls...we do not police them. So far, this works for us. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Wednesday, December 04, 2013 2:51 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP survey Question For a long time I have had our IHC techs print out the run logs from each IHC run on the Benchmark Ultras. The techs then check the slides to make sure the positive and negative controls have worked properly before the slides are sent to the individual pathologists. The pathologists are also supposed to check the controls before looking at the patient slides. Lately in the interest of reducing turnaround time I have been asked why we run the log reports and have a tech look over the controls before they send them to the pathologists since the pathologist will also evaluate the controls. I have been doing this because I wanted the documentation that someone reviewed the controls each time an IHC stain was done. I believe if the pathologists would document someplace that the control slides were reviewed before the patient slides were viewed then I could eliminate the techs looking over the controls also. Problem is how are others documenting that the controls are reviewed? Is this done by the techs, the pathologists, or both? We of course have also used this data for quality assurance of our stains. Thanks for your help. Jim James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SHUNTER <@t> beaumont.edu Thu Dec 5 09:50:06 2013 From: SHUNTER <@t> beaumont.edu (Sue Hunter) Date: Thu Dec 5 09:50:10 2013 Subject: [Histonet] M2D2 and SMAD Message-ID: Good morning Does anyone have suggestions for antibodies for M2D2 and SMAD? We have not been able to get these antibodies to work for us on the Ventana Ultras. If you do have suggestions, protocols and control tissue suggestions would also be appreciated. Thanks Sue Sue Hunter, Supervisor Advanced Diagnostics Beaumont Health System Royal Oak MI 248-898-5146 shunter@beaumont.edu From W.E.J.Hoekert <@t> olvg.nl Thu Dec 5 10:18:12 2013 From: W.E.J.Hoekert <@t> olvg.nl (Hoekert, W.E.J.) Date: Thu Dec 5 10:18:21 2013 Subject: [Histonet] M2D2 and SMAD References: Message-ID: <1190CB05C44B13409483514729C2FC3601F84396@PAIT42.olvg.nl> Hi, SMAD4 is very difficult on the Ventana platform. The only clone that would work on the Ventana to my knowledge(clone EP618Y, immunologic) has recently 'collapsed' (i hope that's the right word?) It means that it is not available anymore. Maybe there is some vendor that has a few vials left, but my supplier does not. If anybody knows of some other clone for the Ventana, I am very interested. MDM2: our sarcoma pathologist says that the MDM2 immuno is not reliable. Some liposarcomas will be negative. It would be better to do FISH, which is also very difficult because the fatty tissue often rinses off the slides. Willem ________________________________ Van: histonet-bounces@lists.utsouthwestern.edu namens Sue Hunter Verzonden: do 5-12-2013 16:50 Aan: Histonet@lists.utsouthwestern.edu Onderwerp: [Histonet] M2D2 and SMAD Good morning Does anyone have suggestions for antibodies for M2D2 and SMAD? We have not been able to get these antibodies to work for us on the Ventana Ultras. If you do have suggestions, protocols and control tissue suggestions would also be appreciated. Thanks Sue Sue Hunter, Supervisor Advanced Diagnostics Beaumont Health System Royal Oak MI 248-898-5146 shunter@beaumont.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Disclaimer: Dit e-mail bericht is uitsluitend bestemd voor de geadresseerde(n). Verstrekking aan en gebruik door anderen dan geadresseerden is niet toegestaan. Indien u niet de geadresseerde bent, wordt u verzocht de verzender hiervan op de hoogte te stellen en het bericht te verwijderen. In verband met electronische verzending kunnen aan dit e-mail bericht geen rechten worden ontleend. From julie.hinsinger <@t> umontreal.ca Thu Dec 5 10:23:52 2013 From: julie.hinsinger <@t> umontreal.ca (Hinsinger Julie) Date: Thu Dec 5 10:23:58 2013 Subject: [Histonet] Aviva antibodies Message-ID: <4E1A2BAF76DB1E4CB35AA132AE0492D209934E1C@ashfield-cour.sim.umontreal.ca> Does anybody know about the quality of Aviva Systems Biology antibodies for IHC ? Thanks for your help. Julie From cls71877 <@t> gmail.com Thu Dec 5 11:45:52 2013 From: cls71877 <@t> gmail.com (Cristi Stephenson) Date: Thu Dec 5 11:45:57 2013 Subject: [Histonet] Shandon Grosslab Senior Message-ID: Hello, Does anyone that uses this grossing station have a great (cheaper than ThermoScientific) place to purchase filters? Thanks, Cristi From vavalos <@t> allergydermatology.com Thu Dec 5 11:55:14 2013 From: vavalos <@t> allergydermatology.com (Vanessa Avalos) Date: Thu Dec 5 11:55:24 2013 Subject: [Histonet] Shandon Grosslab Senior In-Reply-To: References: Message-ID: <1d5086a65a35445ebe96785a807278d6@BY2PR07MB217.namprd07.prod.outlook.com> Mercedes Medical sells their own brand that are compatible with others out there and sell for less than Thermo. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cristi Stephenson Sent: Thursday, December 05, 2013 10:46 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Shandon Grosslab Senior Hello, Does anyone that uses this grossing station have a great (cheaper than ThermoScientific) place to purchase filters? Thanks, Cristi _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Michael.LaFriniere <@t> ccplab.com Thu Dec 5 12:02:17 2013 From: Michael.LaFriniere <@t> ccplab.com (Michael LaFriniere) Date: Thu Dec 5 12:02:27 2013 Subject: [Histonet] Shandon Grosslab Senior In-Reply-To: References: Message-ID: <4A2A16B9707CE04E9CB6C82DC18C1D2963E8F2@AHCMSASEXCH03.my.ahc.local> Christi, I just built a lab and ordered 5 of them through Mopec, they were fantastic to work with and gave discounts for each additional unit purchased throughout the 12 month period that I bought them. Michael Michael R. LaFriniere, HT (ASCP) Executive Director Capital Choice Pathology Laboratory 12041 Bournefield Way, Suite A * Silver Spring, MD 20904 P: 240.471.3427 * F: 240.471.3401 * Cell 410-940-8844 michael.lafriniere@csmlab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cristi Stephenson Sent: Thursday, December 05, 2013 12:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Shandon Grosslab Senior Hello, Does anyone that uses this grossing station have a great (cheaper than ThermoScientific) place to purchase filters? Thanks, Cristi _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wbenton <@t> cua.md Thu Dec 5 12:25:38 2013 From: wbenton <@t> cua.md (Walter Benton) Date: Thu Dec 5 12:26:02 2013 Subject: [Histonet] Shandon Grosslab Senior In-Reply-To: References: Message-ID: <0B8979A204680A42B93A52B486088CD93931ABF0FA@CUAEXH1.GCU-MD.local> Try Mercedes Medical Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) ChesapeakeUrology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cristi Stephenson [cls71877@gmail.com] Sent: Thursday, December 05, 2013 12:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Shandon Grosslab Senior Hello, Does anyone that uses this grossing station have a great (cheaper than ThermoScientific) place to purchase filters? Thanks, Cristi _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From o.m.gallagher <@t> sheffield.ac.uk Thu Dec 5 12:30:34 2013 From: o.m.gallagher <@t> sheffield.ac.uk (Orla M Gallagher) Date: Thu Dec 5 12:30:41 2013 Subject: [Histonet] Bone samples long-term storage in 10% formalin or 4% paraformaldehyde Message-ID: Dear Histonetters, What is your opinion on storing bone samples long-term (more than a couple of weeks) in 10% formalin? As I was taught, best practice has always been to fix only as long as necessary, depending on the size of the sample, then decalcify and process to wax, and I always stress this to everyone I advise. However, research colleagues sometimes wish to do histology on bone samples that have been stored for months ..or even years! As the formalin pH becomes more acidic, there is formalin pigment and the immunoreactivity and TRAP enzyme activity is diminished or destroyed during long fixation, is there any way of minimising this e.g. has anyone tried regularly replacing the old formalin with fresh buffered formalin, or storing formalin-fixed bones in any other medium? I'm also interested in how best to fix in 4% paraformaldehyde and whether the problems are the same with long-term storage. Thanks for your comments. All the best, Orla -- ************************** Ms. Orla Gallagher Bone Analysis Laboratory Mellanby Centre for Bone Research Department of Human Metabolism D Floor Medical School University of Sheffield Beech Hill Road Sheffield S10 2RX UK Website: http://mellanbycentre.dept.shef.ac.uk Tel: 0044114-2713337 (office) 0044114-2713174 (lab) E-Mail: o.m.gallagher@sheffield.ac.uk *STOP*: Do you really need to print this e-mail? *BE GREEN:* Keep it on the screen. *Times Higher Education University of the Year* Data protection and confidentiality: The information contained in this message or any appended documents may be privileged and confidential and is intended for the exclusive use of the addressee(s). If you are not the addressee, any disclosure, reproduction, distributions, other dissemination or use of this message is strictly prohibited and may be unlawful. If you receive this correspondence in error please contact the sender immediately and permanently delete/destroy what you have received. From gu.lang <@t> gmx.at Thu Dec 5 12:42:44 2013 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Thu Dec 5 12:43:30 2013 Subject: AW: [Histonet] Bone samples long-term storage in 10% formalin or 4% paraformaldehyde In-Reply-To: References: Message-ID: <000001cef1e9$c9d5a960$5d80fc20$@gmx.at> Paraformaldehyd is formaldehyd in solid form. Formalin is the aequous solution of formaldehyd. So the main characteristics are the same. Gudrun Lang -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Orla M Gallagher Gesendet: Donnerstag, 05. Dezember 2013 19:31 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Bone samples long-term storage in 10% formalin or 4% paraformaldehyde Dear Histonetters, What is your opinion on storing bone samples long-term (more than a couple of weeks) in 10% formalin? As I was taught, best practice has always been to fix only as long as necessary, depending on the size of the sample, then decalcify and process to wax, and I always stress this to everyone I advise. However, research colleagues sometimes wish to do histology on bone samples that have been stored for months ..or even years! As the formalin pH becomes more acidic, there is formalin pigment and the immunoreactivity and TRAP enzyme activity is diminished or destroyed during long fixation, is there any way of minimising this e.g. has anyone tried regularly replacing the old formalin with fresh buffered formalin, or storing formalin-fixed bones in any other medium? I'm also interested in how best to fix in 4% paraformaldehyde and whether the problems are the same with long-term storage. Thanks for your comments. All the best, Orla -- ************************** Ms. Orla Gallagher Bone Analysis Laboratory Mellanby Centre for Bone Research Department of Human Metabolism D Floor Medical School University of Sheffield Beech Hill Road Sheffield S10 2RX UK Website: http://mellanbycentre.dept.shef.ac.uk Tel: 0044114-2713337 (office) 0044114-2713174 (lab) E-Mail: o.m.gallagher@sheffield.ac.uk *STOP*: Do you really need to print this e-mail? *BE GREEN:* Keep it on the screen. *Times Higher Education University of the Year* Data protection and confidentiality: The information contained in this message or any appended documents may be privileged and confidential and is intended for the exclusive use of the addressee(s). If you are not the addressee, any disclosure, reproduction, distributions, other dissemination or use of this message is strictly prohibited and may be unlawful. If you receive this correspondence in error please contact the sender immediately and permanently delete/destroy what you have received. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From d-emge <@t> northwestern.edu Thu Dec 5 12:47:12 2013 From: d-emge <@t> northwestern.edu (Donna J Emge) Date: Thu Dec 5 12:47:17 2013 Subject: [Histonet] Position in Rosemont, IL - DIRECTOR CENTRAL LABORATORY - HISTOLOGY Message-ID: <54C3042A8564BA4AA2D355DD1D4C949C123BE2FB@CHCSPMBX1.ads.northwestern.edu> Hello Fellow Histonetters, I want to let everyone know about this new position for a Histology Director at ACL laboratories in Rosemont, IL, near Chicago. I just joined ACL a couple of weeks ago. It is very dynamic and a great place to work. Donna Emge, HT(ASCP) Histology Supervisor ACL Laboratories Director Central Laboratory - Histology Requisition: 25014-60814 ACL Laboratories is the result of a progressive consolidation of two major, regional health care systems? laboratory services, managed as one operation. Advocate Health Care (Illinois), and Aurora Health Care (Wisconsin), ACL?s nationally ranked parent systems, share quality, safety, performance and employee engagement values, ethics and accountability. Job Accountabilities This position is responsible for executive leadership of a large, complex, multifaceted Histology operation through the coordination of all technical, logistical, and administrative functions to ensure prompt and accurate testing and reporting of results for clinical laboratory samples within established timeframes. Applicants must possess: ? Bachelor degree in medical field or business management. Advanced classes with commitment to complete advanced degree. ? Minimum of 7 ? 8 years management and technical experience in a medical laboratory/histology environment. ? Proven leadership skills including team building and strong customer focus improvements, familiarity with HCFA and licensing regulations. ? Leadership experience in an integrated or consolidated laboratory preferred. To learn more about the position, along with submitting an application for consideration, please visit http://careers.advocatehealth.com/ and search for Job ID 25014-60814. From kmerriam2003 <@t> yahoo.com Thu Dec 5 13:23:12 2013 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Thu Dec 5 13:23:14 2013 Subject: [Histonet] histology tracking systems for pharma or contract lab Message-ID: <1386271392.31417.YahooMailNeo@web121906.mail.ne1.yahoo.com> Hi, My company is considering a new tracking system for our histology studies. ?This system needs to track everything from trimming, decal, embedding, cutting, routine and molecular pathology/staining. ?It also needs to be able to accommodate complex study designs (multiple animals, groups, necropsy timepoints, etc). I am interesting in hearing from people in other pharma/biotech/CROs to see what they are using to capture this information. Thanks, Kim ? Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From tkngflght <@t> yahoo.com Thu Dec 5 14:10:22 2013 From: tkngflght <@t> yahoo.com (Cheryl Kerry) Date: Thu Dec 5 14:11:37 2013 Subject: [Histonet] Helicobacter helmani -- help? In-Reply-To: References: Message-ID: All - thank you for the education! We're waiting for a definite response from the manufacturer and will offer an Alcian yellow while we wait... Happy holidays!! Sent from my iPhone > On Dec 4, 2013, at 6:01 PM, Milanka Ilic wrote: > > Hi Cheryl, > > The polyclonal Helicobacter pylori (HP) antibody is known to cross-react with H. Heilmannii (HH) (see attached), making it particularly useful in increasing the sensitivity of the polyclonal HP antibody. The monoclonal HP antibody, while high in specificity, lacks in sensitivity as it only stains for HP and no other helicobacter species. Cell Marque offers an IVD polyclonal HP antibody which will detect HH as well. Attached is the flyer with ordering information. > > Please let me know how I can further assist. > > Thank you, > > Milanka Ilic > Technical Consultant > > > 6600 Sierra College Blvd. > Rocklin, CA 95677 > 916.746.8900 x8963 (office) > 916.247.7670 (mobile) > 916.746.8989 (fax) > > > From Passion to Product to Patient.? > > > This message and any files transmitted with it are the property of Cell Marque Corporation, are confidential, and are intended solely for the use of the person or entity to whom this e-mail is addressed. If you are not one of the named recipient(s) or otherwise have reason to believe that you have received this message in error, please contact the sender and delete this message immediately from your computer. Any other use, retention, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. > > ?? > > From b427297 <@t> aol.com Thu Dec 5 14:15:05 2013 From: b427297 <@t> aol.com (b427297@aol.com) Date: Thu Dec 5 14:15:12 2013 Subject: [Histonet] histology tracking systems for pharma or contract lab In-Reply-To: <1386271392.31417.YahooMailNeo@web121906.mail.ne1.yahoo.com> References: <1386271392.31417.YahooMailNeo@web121906.mail.ne1.yahoo.com> Message-ID: <2C9A4E37-13F8-4D68-B438-FB86ACDB03C7@aol.com> xybion is in the process of developing a histology module for Phama histo. We at AbbVie will be using this system. Jackie Sent from my iPhone > On Dec 5, 2013, at 13:23, Kim Merriam wrote: > > Hi, > > My company is considering a new tracking system for our histology studies. This system needs to track everything from trimming, decal, embedding, cutting, routine and molecular pathology/staining. It also needs to be able to accommodate complex study designs (multiple animals, groups, necropsy timepoints, etc). > > I am interesting in hearing from people in other pharma/biotech/CROs to see what they are using to capture this information. > > Thanks, > Kim > > Kim Merriam, MA, HT(ASCP)QIHC > Cambridge, MA > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Joyce.Weems <@t> emoryhealthcare.org Thu Dec 5 14:23:00 2013 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Thu Dec 5 14:23:05 2013 Subject: [Histonet] CDH1 Message-ID: Anyone doing this mutation test by PCR - performed on normal tissue for families with highly penetrant hereditary diffuse gastric cancer? Thanks, j Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From pruegg <@t> ihctech.net Thu Dec 5 14:49:38 2013 From: pruegg <@t> ihctech.net (pruegg@ihctech.net) Date: Thu Dec 5 14:49:43 2013 Subject: AW: [Histonet] Bone samples long-term storage in 10% formalin or 4% paraformaldehyde In-Reply-To: <000001cef1e9$c9d5a960$5d80fc20$@gmx.at> Message-ID: <20131205134938.f86bd30e73b823f57b516b5451216a98.fb3f94f1f4.mailapi@email01.secureserver.net> i would think u are correct in advising formic acid decal and then processing into paraffin for the best protection of the trap enzyme, immunoreactivity, etc. A couple of weeks in formalin should be fine. Paraformaldehyde show be the same as formalin. I do know a way to restore the enzyme activity for TRAP that may have been lost so if u need that let me know. --------- Original Message --------- Subject: AW: [Histonet] Bone samples long-term storage in 10% formalin or 4% paraformaldehyde From: "Gudrun Lang" Date: 12/5/13 11:42 am To: "'Orla M Gallagher'" Cc: histonet@lists.utsouthwestern.edu Paraformaldehyd is formaldehyd in solid form. Formalin is the aequous solution of formaldehyd. So the main characteristics are the same. Gudrun Lang -----Ursprüngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Orla M Gallagher Gesendet: Donnerstag, 05. Dezember 2013 19:31 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Bone samples long-term storage in 10% formalin or 4% paraformaldehyde Dear Histonetters, What is your opinion on storing bone samples long-term (more than a couple of weeks) in 10% formalin? As I was taught, best practice has always been to fix only as long as necessary, depending on the size of the sample, then decalcify and process to wax, and I always stress this to everyone I advise. However, research colleagues sometimes wish to do histology on bone samples that have been stored for months ..or even years! As the formalin pH becomes more acidic, there is formalin pigment and the immunoreactivity and TRAP enzyme activity is diminished or destroyed during long fixation, is there any way of minimising this e.g. has anyone tried regularly replacing the old formalin with fresh buffered formalin, or storing formalin-fixed bones in any other medium? I'm also interested in how best to fix in 4% paraformaldehyde and whether the problems are the same with long-term storage. Thanks for your comments. All the best, Orla -- ************************** Ms. Orla Gallagher Bone Analysis Laboratory Mellanby Centre for Bone Research Department of Human Metabolism D Floor Medical School University of Sheffield Beech Hill Road Sheffield S10 2RX UK Website: http://mellanbycentre.dept.shef.ac.uk Tel: 0044114-2713337 (office) 0044114-2713174 (lab) E-Mail: o.m.gallagher@sheffield.ac.uk *STOP*: Do you really need to print this e-mail? *BE GREEN:* Keep it on the screen. *Times Higher Education University of the Year* Data protection and confidentiality: The information contained in this message or any appended documents may be privileged and confidential and is intended for the exclusive use of the addressee(s). If you are not the addressee, any disclosure, reproduction, distributions, other dissemination or use of this message is strictly prohibited and may be unlawful. If you receive this correspondence in error please contact the sender immediately and permanently delete/destroy what you have received. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Joyce.Weems <@t> emoryhealthcare.org Thu Dec 5 15:37:03 2013 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Thu Dec 5 15:37:29 2013 Subject: [Histonet] RE: CDH1 In-Reply-To: References: Message-ID: Found it at Stanford - so nevermind!! Thanks, j Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Thursday, December 05, 2013 3:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CDH1 Anyone doing this mutation test by PCR - performed on normal tissue for families with highly penetrant hereditary diffuse gastric cancer? Thanks, j Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From madeathridge <@t> pastnashville.com Thu Dec 5 15:50:13 2013 From: madeathridge <@t> pastnashville.com (Mary Ann Deathridge) Date: Thu Dec 5 15:50:59 2013 Subject: [Histonet] BAP1 Message-ID: <65d67178$6acf33ae$4800a4d8$@com> Anyone doing IHC "BAP1" for cutaneous melanocytic tumours, mesothelioma, Spitz tunours. I think Santa Cruz has the antibody. Any tips, tricks or info on the protocol. Thanks in advance. Michael, great to talk with you ! Maryann Deathridge, BS, HT (ASCP) Lab Manager 615-298-4100, Fax: 615-298-4141 From Ashley.Troutman <@t> Vanderbilt.Edu Thu Dec 5 16:03:22 2013 From: Ashley.Troutman <@t> Vanderbilt.Edu (Troutman, Kenneth A) Date: Thu Dec 5 16:03:30 2013 Subject: [Histonet] The Twelve Days of Histo Message-ID: Hello all, I hope the holidays are treating everyone well. (Especially in light of us having the most hazardous job in the country...) Here's some fun for the season: The Twelve Days of Histo TWELVE Medical Directors ELEVEN logs-a-printing TEN residents reading NINE frozen sections EIGHT administrators SEVEN stainers beeping SIX techs complaining FIVE I...H...Cs.... :) FOUR calling docs THREE recuts TWO special stains And a block with an H and EEEEE... Please feel free to sing along. Merry Christmas in Histoland! Ashley Troutman BS, MBA, HT(ASCP) QIHC Histopathology and Immunohistochemistry Supervisor Vanderbilt University Histopathology 1301 Medical Center Drive TVC 4531 Nashville, TN 37232 ashley.troutman@vanderbilt.edu From lori.garcia <@t> medtronic.com Thu Dec 5 16:08:54 2013 From: lori.garcia <@t> medtronic.com (Garcia, Lori, M.Sc.) Date: Thu Dec 5 16:09:04 2013 Subject: [Histonet] RE: The Twelve Days of Histo In-Reply-To: References: Message-ID: <8214022B32A9DE4985A37EA7AE814DA715B321@MSPM1BMSGM41.ent.core.medtronic.com> Love it :) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Troutman, Kenneth A Sent: Thursday, December 05, 2013 2:03 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] The Twelve Days of Histo Hello all, I hope the holidays are treating everyone well. (Especially in light of us having the most hazardous job in the country...) Here's some fun for the season: The Twelve Days of Histo TWELVE Medical Directors ELEVEN logs-a-printing TEN residents reading NINE frozen sections EIGHT administrators SEVEN stainers beeping SIX techs complaining FIVE I...H...Cs.... :) FOUR calling docs THREE recuts TWO special stains And a block with an H and EEEEE... Please feel free to sing along. Merry Christmas in Histoland! Ashley Troutman BS, MBA, HT(ASCP) QIHC Histopathology and Immunohistochemistry Supervisor Vanderbilt University Histopathology 1301 Medical Center Drive TVC 4531 Nashville, TN 37232 ashley.troutman@vanderbilt.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet [CONFIDENTIALITY AND PRIVACY NOTICE] Information transmitted by this email is proprietary to Medtronic and is intended for use only by the individual or entity to which it is addressed, and may contain information that is private, privileged, confidential or exempt from disclosure under applicable law. If you are not the intended recipient or it appears that this mail has been forwarded to you without proper authority, you are notified that any use or dissemination of this information in any manner is strictly prohibited. In such cases, please delete this mail from your records. To view this notice in other languages you can either select the following link or manually copy and paste the link into the address bar of a web browser: http://emaildisclaimer.medtronic.com From patrick.lewis <@t> seattlechildrens.org Thu Dec 5 16:29:09 2013 From: patrick.lewis <@t> seattlechildrens.org (Lewis, Patrick) Date: Thu Dec 5 16:29:27 2013 Subject: [Histonet] Questions about IHC in Frozen Sections Message-ID: <3903BE18914F4440834F0E620415FFCA381FFF6D@PPWEXD01a.childrens.sea.kids> Hi Everyone. I am trying to troubleshoot my IHC on frozen sections. My sections are human tonsil at 7 uM. On charged Superfrost slides. They are stored at -80 after drying for 1 hour. When I use them for IHC, I take them out of the -80 and let them air dry for 1 hour before placing them in cold acetone for 30 minutes to fix. Question: If I place them directly in H20 or TBST pH 8.0 after fixation, will that cause cell lysis? Should I dry the slides after acetone fixation before washing them? If so, for how long? My problem seems to be that the tissue is getting digested on the slide, I am trying to trouble shoot which step is causing my tissues to disintegrate. So far I have tried thicker sections 10, 15 uM (That made the problem worse, I am consider going back to 4 uM sections) I also Changed the concentration of H2O2 for my H202 block from 3% to 0.3%, (In my next IHC attempt I will try to examine the slide at each step to see if I can see loss of integrity) Also in my next attempt I plan to eliminate any H20 washes and dry the slide post acetone fixation before washing in TBST. Also I plan to decrease the amount of Tween20 in my Wash buffer from 0.2% to 0.02%. Any advice would be helpful. Patrick. CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From Haley.Huggins <@t> DignityHealth.org Thu Dec 5 16:38:25 2013 From: Haley.Huggins <@t> DignityHealth.org (Huggins, Haley - MRMC) Date: Thu Dec 5 16:38:31 2013 Subject: [Histonet] RE: The Twelve Days of Histo In-Reply-To: <8214022B32A9DE4985A37EA7AE814DA715B321@MSPM1BMSGM41.ent.core.medtronic.com> References: <8214022B32A9DE4985A37EA7AE814DA715B321@MSPM1BMSGM41.ent.core.medtronic.com> Message-ID: <4F36EC93A5737D4F8A2974E8FB8E260616026D0870@PHX-MSG-007-N2.chw.edu> That is great! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Garcia, Lori, M.Sc. Sent: Thursday, December 05, 2013 2:09 PM To: Troutman, Kenneth A; Histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: The Twelve Days of Histo Love it :) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Troutman, Kenneth A Sent: Thursday, December 05, 2013 2:03 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] The Twelve Days of Histo Hello all, I hope the holidays are treating everyone well. (Especially in light of us having the most hazardous job in the country...) Here's some fun for the season: The Twelve Days of Histo TWELVE Medical Directors ELEVEN logs-a-printing TEN residents reading NINE frozen sections EIGHT administrators SEVEN stainers beeping SIX techs complaining FIVE I...H...Cs.... :) FOUR calling docs THREE recuts TWO special stains And a block with an H and EEEEE... Please feel free to sing along. Merry Christmas in Histoland! Ashley Troutman BS, MBA, HT(ASCP) QIHC Histopathology and Immunohistochemistry Supervisor Vanderbilt University Histopathology 1301 Medical Center Drive TVC 4531 Nashville, TN 37232 ashley.troutman@vanderbilt.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet [CONFIDENTIALITY AND PRIVACY NOTICE] Information transmitted by this email is proprietary to Medtronic and is intended for use only by the individual or entity to which it is addressed, and may contain information that is private, privileged, confidential or exempt from disclosure under applicable law. If you are not the intended recipient or it appears that this mail has been forwarded to you without proper authority, you are notified that any use or dissemination of this information in any manner is strictly prohibited. In such cases, please delete this mail from your records. To view this notice in other languages you can either select the following link or manually copy and paste the link into the address bar of a web browser: http://emaildisclaimer.medtronic.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mucram11 <@t> comcast.net Thu Dec 5 16:46:33 2013 From: mucram11 <@t> comcast.net (Pam Marcum) Date: Thu Dec 5 16:46:48 2013 Subject: [Histonet] The Twelve Days of Histo In-Reply-To: Message-ID: <1787400736.2094474.1386283593119.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Thaks waiting for the? ice storm and sleeping in the lab tonight so we appreciated a good laugh.? Did you notice you have to sing it in your head? Pam Marcum ----- Original Message ----- From: "Kenneth A Troutman" To: Histonet@lists.utsouthwestern.edu Sent: Thursday, December 5, 2013 4:03:22 PM Subject: [Histonet] The Twelve Days of Histo Hello all, I hope the holidays are treating everyone well. ?(Especially in light of us having the most hazardous job in the country...) Here's some fun for the season: The Twelve Days of Histo TWELVE Medical Directors ELEVEN logs-a-printing TEN residents reading NINE frozen sections EIGHT administrators SEVEN stainers beeping SIX techs complaining FIVE I...H...Cs.... ?:) FOUR calling docs THREE recuts TWO special stains And a block with an H and EEEEE... Please feel free to sing along. Merry Christmas in Histoland! Ashley Troutman BS, MBA, HT(ASCP) QIHC Histopathology and Immunohistochemistry Supervisor Vanderbilt University Histopathology 1301 Medical Center Drive TVC 4531 Nashville, TN ?37232 ashley.troutman@vanderbilt.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amosbrooks <@t> gmail.com Thu Dec 5 16:50:47 2013 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Thu Dec 5 16:50:54 2013 Subject: [Histonet] CAP Survey Question Message-ID: <2kmn05fcknpy4f6jtm8wx0n7.1386283847250@email.android.com> Hi, Regarding the question of weather it would save time to not have the techs QC check the IHC slides. I feel that it would be an extremely bad practice to omit the techs from the QC process. This goes for not only IHC but special stains and even H&E stains. It is imperative that the techs know what the slides they are submitting look like. It is possible for even a lowly tech to spot a problem before passing the slide on to a pathologist. And even pathologists are capable of missing something from time to time. This also educates the tech so that when a pathologist has a question about a stain he is not met with a blank stare. So CAP requirement or not it should be done anyway. Just my opinion, (But I'm right), Amos From Haley.Huggins <@t> DignityHealth.org Thu Dec 5 16:56:14 2013 From: Haley.Huggins <@t> DignityHealth.org (Huggins, Haley - MRMC) Date: Thu Dec 5 16:56:22 2013 Subject: [Histonet] RE: Yahoo link In-Reply-To: <63f258ee13dc423ab0531cb811d5d71e@BY2PR07MB469.namprd07.prod.outlook.com> References: <63f258ee13dc423ab0531cb811d5d71e@BY2PR07MB469.namprd07.prod.outlook.com> Message-ID: <4F36EC93A5737D4F8A2974E8FB8E260616026D0884@PHX-MSG-007-N2.chw.edu> We are finally recognized and it is for this. So sad :( -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Tighe Sent: Tuesday, December 03, 2013 6:31 AM To: histonet@lists.utsouthwestern.edu (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Yahoo link Anybody wonder who has the most harzardous job to your health? We're Number One!!! http://finance.yahoo.com/news/the-15-jobs-that-are-most-damaging-to-your-health-155706120.html Mike _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Thu Dec 5 16:58:32 2013 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Thu Dec 5 16:58:38 2013 Subject: [Histonet] RE: Questions about IHC in Frozen Sections In-Reply-To: <3903BE18914F4440834F0E620415FFCA381FFF6D@PPWEXD01a.childrens.sea.kids> References: <3903BE18914F4440834F0E620415FFCA381FFF6D@PPWEXD01a.childrens.sea.kids> Message-ID: <14E2C6176416974295479C64A11CB9AE019C79E04ED1@SBS2K8.premierlab.local> Patrick Here is what we do for frozen IHC, this is based upon methods that I received from Gayle Callis. Cut frozen sections and let air dry - at least 20-30 minutes post the last section cut. If we are going to stain that same day or the following day we leave the slides at room temp (we are pretty dry here in Colorado) but if you have issues with humidity you can store them in a dessicator overnight. If you need to store at -80 then we package the slides in smaller slide boxes and only package enough slides for one run to avoid freeze and thaw artifact. So once the slides have dried we place them in slide boxes and in those slide boxes we add a small or medium nylon tissue bag that contains Silica Gel, 6-16 mesh (indicating) we just staple the nylon bag shut. We then use a food sealer to seal the slide box in one of those food sealing bags (we got ours at Cost Co they have them on sale every once and a while, along with the bags) and then that goes in the -80 for storage. The day before we are going to stain we pull out the sealed slide box from the -80 and let it sit on the counter top until the next morning when we open up and then fix with the best method for the particular IHC that we are going to use it could be 10% NBF or 4% paraformaldehyde or one that Gayle recommended to us - its an ethanol/acetone mixture - the protocol is listed below. 1. Fix for 5 minutes in solution made of 75% Acetone and 25% Absolute Ethyl Alcohol. NOTE: We purchase Absolute Ethyl Alcohol in the small bottles. Both Acetone and Absolute Ethyl Alcohol are both stored in the flammable storage cabinet. 2. Rinse in two buffer changes for at least 2 minutes. 3. Continue with staining protocol. Good Luck Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lewis, Patrick Sent: Thursday, December 05, 2013 3:29 PM To: 'Histonet@lists.utsouthwestern.edu' Subject: [Histonet] Questions about IHC in Frozen Sections Hi Everyone. I am trying to troubleshoot my IHC on frozen sections. My sections are human tonsil at 7 uM. On charged Superfrost slides. They are stored at -80 after drying for 1 hour. When I use them for IHC, I take them out of the -80 and let them air dry for 1 hour before placing them in cold acetone for 30 minutes to fix. Question: If I place them directly in H20 or TBST pH 8.0 after fixation, will that cause cell lysis? Should I dry the slides after acetone fixation before washing them? If so, for how long? My problem seems to be that the tissue is getting digested on the slide, I am trying to trouble shoot which step is causing my tissues to disintegrate. So far I have tried thicker sections 10, 15 uM (That made the problem worse, I am consider going back to 4 uM sections) I also Changed the concentration of H2O2 for my H202 block from 3% to 0.3%, (In my next IHC attempt I will try to examine the slide at each step to see if I can see loss of integrity) Also in my next attempt I plan to eliminate any H20 washes and dry the slide post acetone fixation before washing in TBST. Also I plan to decrease the amount of Tween20 in my Wash buffer from 0.2% to 0.02%. Any advice would be helpful. Patrick. CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From fbozkurt <@t> gmail.com Fri Dec 6 02:10:23 2013 From: fbozkurt <@t> gmail.com (Mehmet Fatih BOZKURT) Date: Fri Dec 6 02:10:29 2013 Subject: [Histonet] treponema spp Message-ID: Hello, Do you have any protocol suggestions for *treponema spp * staining with silver and also suggestion for primary antibody doing ihc. Thanks.. -- Mehmet Fatih BOZKURT, DVM, PhD Afyon Kocatepe University Faculty of Veterinary Medicine Department of Pathology 03030, ANS Campus Afyonkarahisar-TURKEY Tel: +902722281312-16173/16237 From joelleweaver <@t> hotmail.com Fri Dec 6 07:04:01 2013 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Fri Dec 6 07:04:04 2013 Subject: [Histonet] CAP Survey Question In-Reply-To: <2kmn05fcknpy4f6jtm8wx0n7.1386283847250@email.android.com> References: <2kmn05fcknpy4f6jtm8wx0n7.1386283847250@email.android.com> Message-ID: I agree. It is required here on everything. Joelle Weaver MAOM, HTL (ASCP) QIHC Date: Thu, 5 Dec 2013 17:50:47 -0500 From: amosbrooks@gmail.com To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP Survey Question Hi, Regarding the question of weather it would save time to not have the techs QC check the IHC slides. I feel that it would be an extremely bad practice to omit the techs from the QC process. This goes for not only IHC but special stains and even H&E stains. It is imperative that the techs know what the slides they are submitting look like. It is possible for even a lowly tech to spot a problem before passing the slide on to a pathologist. And even pathologists are capable of missing something from time to time. This also educates the tech so that when a pathologist has a question about a stain he is not met with a blank stare. So CAP requirement or not it should be done anyway. Just my opinion, (But I'm right), Amos _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Fri Dec 6 07:07:18 2013 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Fri Dec 6 07:07:22 2013 Subject: [Histonet] CAP Survey Question In-Reply-To: References: <2kmn05fcknpy4f6jtm8wx0n7.1386283847250@email.android.com>, Message-ID: Looking at each slide ( at least controls) is required here, period, for everything. I do not see how sending out stains that didn't work and the rework and would save time? Maybe I missed some point in the thread? I don't see how you could learn without this QC practice, even better if you can review under the microscope with a pathologist. Joelle Weaver MAOM, HTL (ASCP) QIHC From: joelleweaver@hotmail.com To: amosbrooks@gmail.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CAP Survey Question Date: Fri, 6 Dec 2013 13:04:01 +0000 I agree. It is required here on everything. Joelle Weaver MAOM, HTL (ASCP) QIHC Date: Thu, 5 Dec 2013 17:50:47 -0500 From: amosbrooks@gmail.com To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP Survey Question Hi, Regarding the question of weather it would save time to not have the techs QC check the IHC slides. I feel that it would be an extremely bad practice to omit the techs from the QC process. This goes for not only IHC but special stains and even H&E stains. It is imperative that the techs know what the slides they are submitting look like. It is possible for even a lowly tech to spot a problem before passing the slide on to a pathologist. And even pathologists are capable of missing something from time to time. This also educates the tech so that when a pathologist has a question about a stain he is not met with a blank stare. So CAP requirement or not it should be done anyway. Just my opinion, (But I'm right), Amos _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brett_connolly <@t> merck.com Fri Dec 6 08:12:13 2013 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Fri Dec 6 08:12:29 2013 Subject: [Histonet] RE: Questions about IHC in Frozen Sections In-Reply-To: <14E2C6176416974295479C64A11CB9AE019C79E04ED1@SBS2K8.premierlab.local> References: <3903BE18914F4440834F0E620415FFCA381FFF6D@PPWEXD01a.childrens.sea.kids> <14E2C6176416974295479C64A11CB9AE019C79E04ED1@SBS2K8.premierlab.local> Message-ID: I agree with Liz, We usually fix with acetone/ethanol 5-10 min then go right into buffer, but occasionally use 2.0% NBF for some antibodies. Our buffer contains 0.1% Tween and our sections can be anywhere from 8-20um depending on the specific project. I think the 30min in acetone is messing up your morphology. Brett Brett M. Connolly, Ph.D. Principal Scientist, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala Sent: Thursday, December 05, 2013 5:59 PM To: Lewis, Patrick; 'Histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Questions about IHC in Frozen Sections Patrick Here is what we do for frozen IHC, this is based upon methods that I received from Gayle Callis. Cut frozen sections and let air dry - at least 20-30 minutes post the last section cut. If we are going to stain that same day or the following day we leave the slides at room temp (we are pretty dry here in Colorado) but if you have issues with humidity you can store them in a dessicator overnight. If you need to store at -80 then we package the slides in smaller slide boxes and only package enough slides for one run to avoid freeze and thaw artifact. So once the slides have dried we place them in slide boxes and in those slide boxes we add a small or medium nylon tissue bag that contains Silica Gel, 6-16 mesh (indicating) we just staple the nylon bag shut. We then use a food sealer to seal the slide box in one of those food sealing bags (we got ours at Cost Co they have them on sale every once and a while, along with the bags) and then that goes in the -80 for storage. The day before we are going to stain we pull out the sealed slide box from the -80 and let it sit on the counter top until the next morning when we open up and then fix with the best method for the particular IHC that we are going to use it could be 10% NBF or 4% paraformaldehyde or one that Gayle recommended to us - its an ethanol/acetone mixture - the protocol is listed below. 1. Fix for 5 minutes in solution made of 75% Acetone and 25% Absolute Ethyl Alcohol. NOTE: We purchase Absolute Ethyl Alcohol in the small bottles. Both Acetone and Absolute Ethyl Alcohol are both stored in the flammable storage cabinet. 2. Rinse in two buffer changes for at least 2 minutes. 3. Continue with staining protocol. Good Luck Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lewis, Patrick Sent: Thursday, December 05, 2013 3:29 PM To: 'Histonet@lists.utsouthwestern.edu' Subject: [Histonet] Questions about IHC in Frozen Sections Hi Everyone. I am trying to troubleshoot my IHC on frozen sections. My sections are human tonsil at 7 uM. On charged Superfrost slides. They are stored at -80 after drying for 1 hour. When I use them for IHC, I take them out of the -80 and let them air dry for 1 hour before placing them in cold acetone for 30 minutes to fix. Question: If I place them directly in H20 or TBST pH 8.0 after fixation, will that cause cell lysis? Should I dry the slides after acetone fixation before washing them? If so, for how long? My problem seems to be that the tissue is getting digested on the slide, I am trying to trouble shoot which step is causing my tissues to disintegrate. So far I have tried thicker sections 10, 15 uM (That made the problem worse, I am consider going back to 4 uM sections) I also Changed the concentration of H2O2 for my H202 block from 3% to 0.3%, (In my next IHC attempt I will try to examine the slide at each step to see if I can see loss of integrity) Also in my next attempt I plan to eliminate any H20 washes and dry the slide post acetone fixation before washing in TBST. Also I plan to decrease the amount of Tween20 in my Wash buffer from 0.2% to 0.02%. Any advice would be helpful. Patrick. CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From Ronald.Houston <@t> nationwidechildrens.org Fri Dec 6 08:50:51 2013 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Fri Dec 6 08:51:00 2013 Subject: [Histonet] Bartonella PCR Message-ID: Does anyone know where we can get Bartonella PCR done on formaldehyde fixed, paraffin-embedded tissue? Thanks Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org "One person with passion is better than forty people merely interested." ~ E.M. Forster From Taylor.Clifford <@t> va.gov Fri Dec 6 09:09:14 2013 From: Taylor.Clifford <@t> va.gov (Clifford, Taylor) Date: Fri Dec 6 09:10:03 2013 Subject: [Histonet] IHC Research Technician - NY Message-ID: <7FF33CF7105F23409F31E748D906B489A033CEF0BE@R04BYNMSGB2.r04.med.va.gov> Still looking for an technician to join a research team in Albany, NY in a Neuropathology Lab devoted to hereditary ataxia research. Duties include daily manual IHC/staining & selected biomolecular techniques (ELISA, PCR etc.). Looking to fill position immediately to replace associate transitioning to graduate school. Bachelor's in Biology, Neuroscience, Histotechnology or related field preferred but would consider applicants with an Associates with related experience. HT/HTL certification not required. Direct questions to e-mail listed below or Arnulf.Koeppen@va.gov Interested applicants should send resume, 3 references & transcripts to Arnulf.Koeppen@va.gov Taylor CM Clifford Research Associate Albany Research Institute 113 Holland Avenue Albany, NY 12208 518-626-5664 Taylor.Clifford@va.gov From jpiche <@t> wtbyhosp.org Fri Dec 6 09:46:08 2013 From: jpiche <@t> wtbyhosp.org (Piche, Jessica) Date: Fri Dec 6 09:46:16 2013 Subject: [Histonet] CAP Survey Question In-Reply-To: References: <2kmn05fcknpy4f6jtm8wx0n7.1386283847250@email.android.com>, Message-ID: <631955447A364B45B9458D29056351109630ED35@WIN08-MBX-01.wtbyhosp.org> More viewing with the pathologist would be great! Jessica Piche, HT(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelle weaver Sent: Friday, December 06, 2013 8:07 AM To: Amos Brooks; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CAP Survey Question Looking at each slide ( at least controls) is required here, period, for everything. I do not see how sending out stains that didn't work and the rework and would save time? Maybe I missed some point in the thread? I don't see how you could learn without this QC practice, even better if you can review under the microscope with a pathologist. Joelle Weaver MAOM, HTL (ASCP) QIHC From: joelleweaver@hotmail.com To: amosbrooks@gmail.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CAP Survey Question Date: Fri, 6 Dec 2013 13:04:01 +0000 I agree. It is required here on everything. Joelle Weaver MAOM, HTL (ASCP) QIHC Date: Thu, 5 Dec 2013 17:50:47 -0500 From: amosbrooks@gmail.com To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP Survey Question Hi, Regarding the question of weather it would save time to not have the techs QC check the IHC slides. I feel that it would be an extremely bad practice to omit the techs from the QC process. This goes for not only IHC but special stains and even H&E stains. It is imperative that the techs know what the slides they are submitting look like. It is possible for even a lowly tech to spot a problem before passing the slide on to a pathologist. And even pathologists are capable of missing something from time to time. This also educates the tech so that when a pathologist has a question about a stain he is not met with a blank stare. So CAP requirement or not it should be done anyway. Just my opinion, (But I'm right), Amos _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital From Terra.Wineman <@t> novusint.com Fri Dec 6 10:12:14 2013 From: Terra.Wineman <@t> novusint.com (Wineman, Terra) Date: Fri Dec 6 10:12:42 2013 Subject: AW: [Histonet] Bone samples long-term storage in 10% formalin or 4% paraformaldehyde In-Reply-To: <20131205134938.f86bd30e73b823f57b516b5451216a98.fb3f94f1f4.mailapi@email01.secureserver.net> References: <000001cef1e9$c9d5a960$5d80fc20$@gmx.at> <20131205134938.f86bd30e73b823f57b516b5451216a98.fb3f94f1f4.mailapi@email01.secureserver.net> Message-ID: <1EB8F245A303564EADF12AC7022FA74D70F43E28@NOVUS-EX01.novusint.com> I would suggest a different protocol if the tissue will not be processed for a while. I would say a week in 10%NBF and then transfer the bones to an EDTA decal solution. The bones will decal slowly without the affects of the formic acid. I am in research and this is what we do with our bones. Terra Wineman, HTL (ASCP)CM Research Biologist 636-926-7476 phone terra.wineman@novusint.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of pruegg@ihctech.net Sent: Thursday, December 05, 2013 2:50 PM To: gu.lang@gmx.at; 'Orla M Gallagher' Cc: histonet@lists.utsouthwestern.edu Subject: RE: AW: [Histonet] Bone samples long-term storage in 10% formalin or 4% paraformaldehyde i would think u are correct in advising formic acid decal and then processing into paraffin for the best protection of the trap enzyme, immunoreactivity, etc. A couple of weeks in formalin should be fine. Paraformaldehyde show be the same as formalin. I do know a way to restore the enzyme activity for TRAP that may have been lost so if u need that let me know. --------- Original Message --------- Subject: AW: [Histonet] Bone samples long-term storage in 10% formalin or 4% paraformaldehyde From: "Gudrun Lang" Date: 12/5/13 11:42 am To: "'Orla M Gallagher'" Cc: histonet@lists.utsouthwestern.edu Paraformaldehyd is formaldehyd in solid form. Formalin is the aequous solution of formaldehyd. So the main characteristics are the same. Gudrun Lang -----Ursprüngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Orla M Gallagher Gesendet: Donnerstag, 05. Dezember 2013 19:31 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Bone samples long-term storage in 10% formalin or 4% paraformaldehyde Dear Histonetters, What is your opinion on storing bone samples long-term (more than a couple of weeks) in 10% formalin? As I was taught, best practice has always been to fix only as long as necessary, depending on the size of the sample, then decalcify and process to wax, and I always stress this to everyone I advise. However, research colleagues sometimes wish to do histology on bone samples that have been stored for months ..or even years! As the formalin pH becomes more acidic, there is formalin pigment and the immunoreactivity and TRAP enzyme activity is diminished or destroyed during long fixation, is there any way of minimising this e.g. has anyone tried regularly replacing the old formalin with fresh buffered formalin, or storing formalin-fixed bones in any other medium? I'm also interested in how best to fix in 4% paraformaldehyde and whether the problems are the same with long-term storage. Thanks for your comments. All the best, Orla -- ************************** Ms. Orla Gallagher Bone Analysis Laboratory Mellanby Centre for Bone Research Department of Human Metabolism D Floor Medical School University of Sheffield Beech Hill Road Sheffield S10 2RX UK Website: http://mellanbycentre.dept.shef.ac.uk Tel: 0044114-2713337 (office) 0044114-2713174 (lab) E-Mail: o.m.gallagher@sheffield.ac.uk *STOP*: Do you really need to print this e-mail? *BE GREEN:* Keep it on the screen. *Times Higher Education University of the Year* Data protection and confidentiality: The information contained in this message or any appended documents may be privileged and confidential and is intended for the exclusive use of the addressee(s). If you are not the addressee, any disclosure, reproduction, distributions, other dissemination or use of this message is strictly prohibited and may be unlawful. If you receive this correspondence in error please contact the sender immediately and permanently delete/destroy what you have received. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jenn.Sands <@t> EssentiaHealth.org Fri Dec 6 11:59:06 2013 From: Jenn.Sands <@t> EssentiaHealth.org (Sands, Jenn) Date: Fri Dec 6 11:59:16 2013 Subject: [Histonet] Staffing benchmarks for histology Message-ID: <6009E599632EF9439B3FE1CBE9716484B58401@turkey.medcampus.org> I am trying to find the pdf/full text article for Staffing Benchmarks for Histology. We are working to determine workload limits for our PA and this article looks like it may assist us. Does anyone have access to this article. I do not have anyone who is a subscriber to the Annals of Diagnostic Pathology. Thanks so much for your help! Jenn Jenn Sands Anatomic Pathology Supervisor Jenn.Sands@essentiahealth.org This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. As required by federal and state laws, you need to hold this information as privileged and confidential. If you have received this communication in error, please notify the sender and destroy all copies of this communication and any attachments. From gayle.callis <@t> bresnan.net Fri Dec 6 16:29:05 2013 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Fri Dec 6 16:29:20 2013 Subject: [Histonet] Re: Questions about IHC on Frozen Sections Message-ID: <001001cef2d2$948d1150$bda733f0$@bresnan.net> Dear Brett, Liz and Patrick, I agree with Brett and Liz having been in contact with them over the years. However if your tissue is of human origin and you want to do CD4 and/or CD8 staining, then the acetone/alcohol fixative should NOT be used. The alcohol will ruin Human CD4 and CD8 antigens but does not harm mouse or rat CD4 or CD8 antigen. I learned this from Dr. Chris van der Loos who is now going to be sorely missed by the immunostaining community. For human CD4 and CD8, the sections should be air dried, then fixed in cold 4C reagent grade acetone for 10 minutes, then air dried to let the acetone evaporate before going into the buffer. I never rinsed my solvent fixed frozen sections in water, and if the buffer is not made correctly, as I learned the hard way, the sections can look horrible. Being a purist, my acetone fixed FS were rinsed in 3 changes of pure buffer before equilibrating with in protocol rinse buffer/0.05% Tween 20. IF YOU DO USE the acetone/alochol fixation, the sections are fixed at RT in this mixture and then go directly into buffer for 3 changes. DO NOT AIR DRY AFTER THIS Acetone/alcohol mixture/fixative. If you fix a long, long time in acetone, you can get lesser staining of the antigen. Another clever trick is doing a double cold acetone fixation of air dried frozen sections. This stabilizes the section so that it stays on the slide better, and doesn't harm the antigens. It would work for murine and human FS. Procedure is: Fix air dry section for 10 min in 4C acetone, remove and air dry section for 10 minutes, then return for fix again for 10 min in 4C acetone, then air dry these sections to evaporate away the acetone approx 10 to 15 minutes, rinse in pure buffer, proceed with staining. Do NOT rinse your solvent fixed ( or air dried, unfixed) frozen sections with water (the enemy!), you want to use buffer to maintain isotonicity and cellular integrity of the solvent fixed FS. At the end of a chromogenic protocol (after the chromogen is developed), you can even rinse with pure buffer, then immerse the stained sections into NBF to post fix the section for 10 minutes, rinse gently with running water and then counter stain with hematoxylin. This is also a van der Loos trick to improve the cellular morphology of the nuclei in solvent fixed FS, and doesn't harm the chromogen. Why do you use TBSTw at pH 8? That pH seems to be a big high for IHC, as the norm tends to be pH 7.6? You can also make up this endogenous peroxidase block that will NOT chew your sections up. Solvent fixed frozen sections do NOT like strong hydrogen peroxide concentrations, and this one worked perfectly for us. It is also a published method. PEROXIDASE BLOCK (0.03% hydrogen peroxide) 5 mls DPBS (Dulbeccos, Sigma), pH 7.4 - 7.4 5 ul 30% hydrogen peroxide 50 ul 10% sodium azide Make up, put in a dropper bottle, and use for 1 week, refrigerate. Discard after 1 week or make up fresh daily. Add to section, incubate for 10 - 15 minutes at RT, rinse well after blocking. If you wish, you can drain off the block, and add new half way through the block if the tissue is particularly bloody. If you think the peroxidase block is still too strong, simply do Alkaline phosphatase methods instead. Always let your unfixed frozen sections just taken from -80C freezer, equilibrate for 20 minutes or more to RT before opening a box as water condensation is the enemy to both antigens and morphology. I am sure I have repeated a great deal of what Liz and Brett presented, but it does drive home some points. Take care Gayle Callis HTL,HT/MT (ASCP) -----Original Message--------------------- I agree with Liz, We usually fix with acetone/ethanol 5-10 min then go right into buffer, but occasionally use 2.0% NBF for some antibodies. Our buffer contains 0.1% Tween and our sections can be anywhere from 8-20um depending on the specific project. I think the 30min in acetone is messing up your morphology. Brett Brett M. Connolly, Ph.D. Principal Scientist, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly <@t> merck.com T- 215-652-2501 F- 215-993-6803 -----Original Message----- From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto: histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala Sent: Thursday, December 05, 2013 5:59 PM To: Lewis, Patrick; ' Histonet <@t> lists.utsouthwestern.edu' Subject: [Histonet] RE: Questions about IHC in Frozen Sections Patrick Here is what we do for frozen IHC, this is based upon methods that I received from Gayle Callis. Cut frozen sections and let air dry - at least 20-30 minutes post the last section cut. If we are going to stain that same day or the following day we leave the slides at room temp (we are pretty dry here in Colorado) but if you have issues with humidity you can store them in a dessicator overnight. If you need to store at -80 then we package the slides in smaller slide boxes and only package enough slides for one run to avoid freeze and thaw artifact. So once the slides have dried we place them in slide boxes and in those slide boxes we add a small or medium nylon tissue bag that contains Silica Gel, 6-16 mesh (indicating) we just staple the nylon bag shut. We then use a food sealer to seal the slide box in one of those food sealing bags (we got ours at Cost Co they have them on sale every once and a while, along with the bags) and then that goes in the -80 for storage. The day before we are going to stain we pull out the sealed slide box from the -80 and let it sit on the counter top until the next morning when we open up and then fix with the best method for the particular IHC that we are going to use it could be 10% NBF or 4% paraformaldehyde or one that Gayle recommended to us - its an ethanol/acetone mixture - the protocol is listed below. 1. Fix for 5 minutes in solution made of 75% Acetone and 25% Absolute Ethyl Alcohol. NOTE: We purchase Absolute Ethyl Alcohol in the small bottles. Both Acetone and Absolute Ethyl Alcohol are both stored in the flammable storage cabinet. 2. Rinse in two buffer changes for at least 2 minutes. 3. Continue with staining protocol. Good Luck Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz <@t> premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto: histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Lewis, Patrick Sent: Thursday, December 05, 2013 3:29 PM To: ' Histonet <@t> lists.utsouthwestern.edu' Subject: [Histonet] Questions about IHC in Frozen Sections Hi Everyone. I am trying to troubleshoot my IHC on frozen sections. My sections are human tonsil at 7 uM. On charged Superfrost slides. They are stored at -80 after drying for 1 hour. When I use them for IHC, I take them out of the -80 and let them air dry for 1 hour before placing them in cold acetone for 30 minutes to fix. Question: If I place them directly in H20 or TBST pH 8.0 after fixation, will that cause cell lysis? Should I dry the slides after acetone fixation before washing them? If so, for how long? My problem seems to be that the tissue is getting digested on the slide, I am trying to trouble shoot which step is causing my tissues to disintegrate. So far I have tried thicker sections 10, 15 uM (That made the problem worse, I am consider going back to 4 uM sections) I also Changed the concentration of H2O2 for my H202 block from 3% to 0.3%, (In my next IHC attempt I will try to examine the slide at each step to see if I can see loss of integrity) Also in my next attempt I plan to eliminate any H20 washes and dry the slide post acetone fixation before washing in TBST. Also I plan to decrease the amount of Tween20 in my Wash buffer from 0.2% to 0.02%. Any advice would be helpful. Patrick. From jamie.erickson <@t> abbvie.com Sun Dec 8 15:41:00 2013 From: jamie.erickson <@t> abbvie.com (Erickson, Jamie E) Date: Sun Dec 8 15:41:11 2013 Subject: [Histonet] CD4 and CD8 background in mouse tumors frozen sections, help Message-ID: <8B946A68A8F3534A99CC493DEFB49B101763C875@WM10005P.oneabbott.com> Hello Histonetters, First I would like to say happy holidays to you all, this is such a great resource to have. Now for my question. I'm rather vexed about this problem with staining CD4/Cd8 in mouse tumors I hope someone can shed light on because I'm stumped. An investigator gave me some frozen mouse tumors and spleen to do CD4 and CD8 stain on and I expected this to be easy... After staining the spleens and tumor with CD4 and CD8 at (1ug/ml and 5ug/ml respectively) I saw the problem, the isotype control. The isotype for these 2 Rat-mouse antibodies from BD biosciences is a Rat IgG2a. The rat IgG2a on the mouse tumor and spleen slide were negative and I was happy but when I looked at the other tumors Rat IgG2a slide some were very positive. The CD4 was staining in some tumors but these tumors had background throughout the tissue as well. Very strange I thought, some tumors no background others lots of background.. As I'm not a tumor biologist I'll just say these tumors vary in size from small to large, large (>1cm) and some have necrosis others do not. So what I see is that the isotype (rat IgG2a) stained in some tumors but not all of them and the spleen and a few small tumors are negative. I only did Neg controls on 2 tumors one large and one small tumor with a spleen from that tumor bearing mouse. I first thought peroxidase or Fc binding as I don't do a protein blocking step with this system, typically. I added a protein block (Dako) and increase peroxidase time from 5 minute to 10 but saw no difference. It looked like my titers were high for these tumors so I titered the antibody out. I titered my CD4 down to 0.25ug/ml for these tumors and that did not help the Rat IgG2a staining but the CD4 staining looked better not as dark brown in the spleen. So now I need to explain my staining protocol.. We use a Leica Bond RX system which I use a DAB kit to detect with and it works great. Marker (antibody, Rat Anti-mouse CD4) 15 Rabbit Anti-Rat vector lab (adsorbed to mouse) as a linking step. 20 polymer (Anti-Rabbit) 8 Peroxide block 5 DAB 10 Hematoxylin 5 I next looked at the linker step slide #1 I did a slide with no CD4.... still had staining (background) Slide #2 No CD4 and No linker ........No background.. clean....I think it is the linker.. Unfortunately I'm stuck using this linker (rabbit anti-Rat , 2ug/ml) for now.. What could be going on in these tumors ??? could I block this without changing my polymer (rabbit). If it is the linker then is it binding via Rabbit Fc or is the hypervariable region specific for something in these tumors i.e.... Anti-Rat tumor protein? Can I pre-complex the CD4 and linker then bind up excess anti-Rat, any one done that??? If it is not complicated enough the linker is adsorbed to mouse. I tried Biocares Rat-on mouse polymer but I did not see any reaction at all, ..but I haven't investigated that fully.. Sorry for the long email .. Hope you can help...Happy holidays. Jamie Erickson AbbVie BioResearch Center Pharmacology 100 Research Drive Worcester, MA 01605 OFFICE +1 508-688-3749 EMAIL terry.melim@abbvie.com From ggracie <@t> stvincents.com.au Sun Dec 8 17:42:55 2013 From: ggracie <@t> stvincents.com.au (Gary Gracie) Date: Sun Dec 8 17:43:08 2013 Subject: [Histonet] treponema Message-ID: <5ac754c9a098415fb484a7b5ab122065@SVMHSEXCH02.svmhs.stvincents.com.au> Dear Mehmet, We are using abcam Treponema ab20923 Regards Gary Gracie Senior Technical Officer IHC Laboratory Anatomical Pathology St Vincents Hospital Sydney Australia Hello, Do you have any protocol suggestions for *treponema spp * staining with silver and also suggestion for primary antibody doing ihc. Thanks.. -- Mehmet Fatih BOZKURT, DVM, PhD Afyon Kocatepe University Faculty of Veterinary Medicine Department of Pathology 03030, ANS Campus Afyonkarahisar-TURKEY Tel: +902722281312-16173/16237 ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the system manager. This footnote also confirms that this email message has been virus scanned and although no viruses were detected by the system, St Vincents & Mater Health Sydney accepts no liability for any consequential damage resulting from email containing any computer viruses. ********************************************************************** From o.m.gallagher <@t> sheffield.ac.uk Mon Dec 9 07:44:47 2013 From: o.m.gallagher <@t> sheffield.ac.uk (Orla M Gallagher) Date: Mon Dec 9 07:44:57 2013 Subject: AW: [Histonet] Bone samples long-term storage in 10% formalin or 4% paraformaldehyde In-Reply-To: <1EB8F245A303564EADF12AC7022FA74D70F43E28@NOVUS-EX01.novusint.com> References: <000001cef1e9$c9d5a960$5d80fc20$@gmx.at> <20131205134938.f86bd30e73b823f57b516b5451216a98.fb3f94f1f4.mailapi@email01.secureserver.net> <1EB8F245A303564EADF12AC7022FA74D70F43E28@NOVUS-EX01.novusint.com> Message-ID: Thanks to everyone for your comments. I may not have been clear in my question - our researchers don't wish to decalcify these formalin-fixed bones yet, but rather to store them for more than a couple of weeks, in case they need to carry out MicroCT followed by histology later. I'm aware that the formalin or paraformaldehyde will degrade over time, but I just wondered if anyone has a protocol for storage without decalcification? I guess transfer to 70% ethanol is an option but this is also not ideal for longterm storage, and would need to be removed before decal in EDTA. All the best, Orla On 6 December 2013 16:12, Wineman, Terra wrote: > I would suggest a different protocol if the tissue will not be processed > for a while. I would say a week in 10%NBF and then transfer the bones to > an EDTA decal solution. The bones will decal slowly without the affects of > the formic acid. I am in research and this is what we do with our bones. > > Terra Wineman, HTL (ASCP)CM > Research Biologist > 636-926-7476 phone > terra.wineman@novusint.com > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of pruegg@ihctech.net > Sent: Thursday, December 05, 2013 2:50 PM > To: gu.lang@gmx.at; 'Orla M Gallagher' > Cc: histonet@lists.utsouthwestern.edu > Subject: RE: AW: [Histonet] Bone samples long-term storage in 10% formalin > or 4% paraformaldehyde > > i would think u are correct in advising formic acid decal and then > processing into paraffin for the best protection of the trap enzyme, > immunoreactivity, etc. A couple of weeks in formalin should be fine. > Paraformaldehyde show be the same as formalin. I do know a way to restore > the enzyme activity for TRAP that may have been lost so if u need that let > me know. > > --------- Original Message --------- Subject: AW: [Histonet] Bone samples > long-term storage in 10% formalin or 4% paraformaldehyde > From: "Gudrun Lang" > Date: 12/5/13 11:42 am > To: "'Orla M Gallagher'" > Cc: histonet@lists.utsouthwestern.edu > > Paraformaldehyd is formaldehyd in solid form. Formalin is the aequous > solution of formaldehyd. > So the main characteristics are the same. > > Gudrun Lang > > -----Ursprüngliche Nachricht----- > Von: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Orla M > Gallagher > Gesendet: Donnerstag, 05. Dezember 2013 19:31 > An: histonet@lists.utsouthwestern.edu > Betreff: [Histonet] Bone samples long-term storage in 10% formalin or 4% > paraformaldehyde > > Dear Histonetters, > > What is your opinion on storing bone samples long-term (more than a > couple of weeks) in 10% formalin? As I was taught, best practice has > always been to fix only as long as necessary, depending on the size of the > sample, then decalcify and process to wax, and I always stress this to > everyone I advise. > > However, research colleagues sometimes wish to do histology on bone > samples that have been stored for months ..or even years! As the formalin > pH becomes more acidic, there is formalin pigment and the immunoreactivity > and TRAP enzyme activity is diminished or destroyed during long fixation, > is there any way of minimising this e.g. has anyone tried regularly > replacing the old formalin with fresh buffered formalin, or storing > formalin-fixed bones in any other medium? I'm also interested in how best > to fix in 4% paraformaldehyde and whether the problems are the same with > long-term storage. > > Thanks for your comments. > > All the best, > Orla > > -- > ************************** > Ms. Orla Gallagher > Bone Analysis Laboratory > Mellanby Centre for Bone Research > Department of Human Metabolism > D Floor Medical School > University of Sheffield > Beech Hill Road > Sheffield > S10 2RX > UK > > Website: http://mellanbycentre.dept.shef.ac.uk > > Tel: 0044114-2713337 (office) > 0044114-2713174 (lab) > E-Mail: o.m.gallagher@sheffield.ac.uk > > > *STOP*: Do you really need to print this e-mail? > > *BE GREEN:* Keep it on the screen. > > > *Times Higher Education University of the Year* > > > > Data protection and confidentiality: > The information contained in this message or any appended documents may > be privileged and confidential and is intended for the exclusive use of > the addressee(s). If you are not the addressee, any disclosure, > reproduction, distributions, other dissemination or use of this message is > strictly prohibited and may be unlawful. If you receive this > correspondence in error please contact the sender immediately and > permanently delete/destroy what you have received. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- ************************** Ms. Orla Gallagher Bone Analysis Laboratory Mellanby Centre for Bone Research Department of Human Metabolism D Floor Medical School University of Sheffield Beech Hill Road Sheffield S10 2RX UK Website: http://mellanbycentre.dept.shef.ac.uk Tel: 0044114-2713337 (office) 0044114-2713174 (lab) E-Mail: o.m.gallagher@sheffield.ac.uk *STOP*: Do you really need to print this e-mail? *BE GREEN:* Keep it on the screen. *Times Higher Education University of the Year* Data protection and confidentiality: The information contained in this message or any appended documents may be privileged and confidential and is intended for the exclusive use of the addressee(s). If you are not the addressee, any disclosure, reproduction, distributions, other dissemination or use of this message is strictly prohibited and may be unlawful. If you receive this correspondence in error please contact the sender immediately and permanently delete/destroy what you have received. From barryrittman <@t> gmail.com Mon Dec 9 07:49:57 2013 From: barryrittman <@t> gmail.com (Barry Rittman) Date: Mon Dec 9 07:50:03 2013 Subject: AW: [Histonet] Bone samples long-term storage in 10% formalin or 4% paraformaldehyde In-Reply-To: References: <000001cef1e9$c9d5a960$5d80fc20$@gmx.at> <20131205134938.f86bd30e73b823f57b516b5451216a98.fb3f94f1f4.mailapi@email01.secureserver.net> <1EB8F245A303564EADF12AC7022FA74D70F43E28@NOVUS-EX01.novusint.com> Message-ID: hi I would recommend storage for long term in 70% ethanol. To prevent drying out we used glycerin in the ethanol, about 20% of the volume. Barry On Mon, Dec 9, 2013 at 7:44 AM, Orla M Gallagher < o.m.gallagher@sheffield.ac.uk> wrote: > Thanks to everyone for your comments. > > I may not have been clear in my question - our researchers don't wish to > decalcify these formalin-fixed bones yet, but rather to store them for more > than a couple of weeks, in case they need to carry out MicroCT followed by > histology later. I'm aware that the formalin or paraformaldehyde will > degrade over time, but I just wondered if anyone has a protocol for storage > without decalcification? I guess transfer to 70% ethanol is an option but > this is also not ideal for longterm storage, and would need to be removed > before decal in EDTA. > > All the best, > Orla > > > On 6 December 2013 16:12, Wineman, Terra > wrote: > > > I would suggest a different protocol if the tissue will not be processed > > for a while. I would say a week in 10%NBF and then transfer the bones to > > an EDTA decal solution. The bones will decal slowly without the affects > of > > the formic acid. I am in research and this is what we do with our bones. > > > > Terra Wineman, HTL (ASCP)CM > > Research Biologist > > 636-926-7476 phone > > terra.wineman@novusint.com > > > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > pruegg@ihctech.net > > Sent: Thursday, December 05, 2013 2:50 PM > > To: gu.lang@gmx.at; 'Orla M Gallagher' > > Cc: histonet@lists.utsouthwestern.edu > > Subject: RE: AW: [Histonet] Bone samples long-term storage in 10% > formalin > > or 4% paraformaldehyde > > > > i would think u are correct in advising formic acid decal and then > > processing into paraffin for the best protection of the trap enzyme, > > immunoreactivity, etc. A couple of weeks in formalin should be fine. > > Paraformaldehyde show be the same as formalin. I do know a way to > restore > > the enzyme activity for TRAP that may have been lost so if u need that > let > > me know. > > > > --------- Original Message --------- Subject: AW: [Histonet] Bone samples > > long-term storage in 10% formalin or 4% paraformaldehyde > > From: "Gudrun Lang" > > Date: 12/5/13 11:42 am > > To: "'Orla M Gallagher'" > > Cc: histonet@lists.utsouthwestern.edu > > > > Paraformaldehyd is formaldehyd in solid form. Formalin is the aequous > > solution of formaldehyd. > > So the main characteristics are the same. > > > > Gudrun Lang > > > > -----Ursprüngliche Nachricht----- > > Von: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Orla > M > > Gallagher > > Gesendet: Donnerstag, 05. Dezember 2013 19:31 > > An: histonet@lists.utsouthwestern.edu > > Betreff: [Histonet] Bone samples long-term storage in 10% formalin or 4% > > paraformaldehyde > > > > Dear Histonetters, > > > > What is your opinion on storing bone samples long-term (more than a > > couple of weeks) in 10% formalin? As I was taught, best practice has > > always been to fix only as long as necessary, depending on the size of > the > > sample, then decalcify and process to wax, and I always stress this to > > everyone I advise. > > > > However, research colleagues sometimes wish to do histology on bone > > samples that have been stored for months ..or even years! As the > formalin > > pH becomes more acidic, there is formalin pigment and the > immunoreactivity > > and TRAP enzyme activity is diminished or destroyed during long > fixation, > > is there any way of minimising this e.g. has anyone tried regularly > > replacing the old formalin with fresh buffered formalin, or storing > > formalin-fixed bones in any other medium? I'm also interested in how > best > > to fix in 4% paraformaldehyde and whether the problems are the same with > > long-term storage. > > > > Thanks for your comments. > > > > All the best, > > Orla > > > > -- > > ************************** > > Ms. Orla Gallagher > > Bone Analysis Laboratory > > Mellanby Centre for Bone Research > > Department of Human Metabolism > > D Floor Medical School > > University of Sheffield > > Beech Hill Road > > Sheffield > > S10 2RX > > UK > > > > Website: http://mellanbycentre.dept.shef.ac.uk > > > > Tel: 0044114-2713337 (office) > > 0044114-2713174 (lab) > > E-Mail: o.m.gallagher@sheffield.ac.uk > > > > > > *STOP*: Do you really need to print this e-mail? > > > > *BE GREEN:* Keep it on the screen. > > > > > > *Times Higher Education University of the Year* > > > > > > > > Data protection and confidentiality: > > The information contained in this message or any appended documents may > > be privileged and confidential and is intended for the exclusive use of > > the addressee(s). If you are not the addressee, any disclosure, > > reproduction, distributions, other dissemination or use of this message > is > > strictly prohibited and may be unlawful. If you receive this > > correspondence in error please contact the sender immediately and > > permanently delete/destroy what you have received. > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > -- > ************************** > Ms. Orla Gallagher > Bone Analysis Laboratory > Mellanby Centre for Bone Research > Department of Human Metabolism > D Floor Medical School > University of Sheffield > Beech Hill Road > Sheffield > S10 2RX > UK > > Website: http://mellanbycentre.dept.shef.ac.uk > > Tel: 0044114-2713337 (office) > 0044114-2713174 (lab) > E-Mail: o.m.gallagher@sheffield.ac.uk > > > *STOP*: Do you really need to print this e-mail? > > *BE GREEN:* Keep it on the screen. > > > *Times Higher Education University of the Year* > > > > Data protection and confidentiality: > The information contained in this message or any appended documents may be > privileged and confidential and is intended for the exclusive use of the > addressee(s). If you are not the addressee, any disclosure, reproduction, > distributions, other dissemination or use of this message is strictly > prohibited and may be unlawful. If you receive this correspondence in error > please contact the sender immediately and permanently delete/destroy what > you have received. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From sadrew <@t> wisc.edu Mon Dec 9 08:51:54 2013 From: sadrew <@t> wisc.edu (Sally Ann Drew) Date: Mon Dec 9 08:52:00 2013 Subject: AW: [Histonet] Bone samples long-term storage in 10% formalin or 4% paraformaldehyde In-Reply-To: References: <000001cef1e9$c9d5a960$5d80fc20$@gmx.at> <20131205134938.f86bd30e73b823f57b516b5451216a98.fb3f94f1f4.mailapi@email01.secureserver.net> <1EB8F245A303564EADF12AC7022FA74D70F43E28@NOVUS-EX01.novusint.com> Message-ID: <007a01cef4ee$338b6410$9aa22c30$@wisc.edu> Just curious-what do people consider the time frame for "long term" ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barry Rittman Sent: Monday, December 09, 2013 7:50 AM To: Orla M Gallagher Cc: histonet@lists.utsouthwestern.edu; Wineman, Terra Subject: Re: AW: [Histonet] Bone samples long-term storage in 10% formalin or 4% paraformaldehyde hi I would recommend storage for long term in 70% ethanol. To prevent drying out we used glycerin in the ethanol, about 20% of the volume. Barry On Mon, Dec 9, 2013 at 7:44 AM, Orla M Gallagher < o.m.gallagher@sheffield.ac.uk> wrote: > Thanks to everyone for your comments. > > I may not have been clear in my question - our researchers don't wish > to decalcify these formalin-fixed bones yet, but rather to store them > for more than a couple of weeks, in case they need to carry out > MicroCT followed by histology later. I'm aware that the formalin or > paraformaldehyde will degrade over time, but I just wondered if anyone > has a protocol for storage without decalcification? I guess transfer > to 70% ethanol is an option but this is also not ideal for longterm > storage, and would need to be removed before decal in EDTA. > > All the best, > Orla > > > On 6 December 2013 16:12, Wineman, Terra > wrote: > > > I would suggest a different protocol if the tissue will not be > > processed for a while. I would say a week in 10%NBF and then > > transfer the bones to an EDTA decal solution. The bones will decal > > slowly without the affects > of > > the formic acid. I am in research and this is what we do with our bones. > > > > Terra Wineman, HTL (ASCP)CM > > Research Biologist > > 636-926-7476 phone > > terra.wineman@novusint.com > > > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > pruegg@ihctech.net > > Sent: Thursday, December 05, 2013 2:50 PM > > To: gu.lang@gmx.at; 'Orla M Gallagher' > > Cc: histonet@lists.utsouthwestern.edu > > Subject: RE: AW: [Histonet] Bone samples long-term storage in 10% > formalin > > or 4% paraformaldehyde > > > > i would think u are correct in advising formic acid decal and then > > processing into paraffin for the best protection of the trap enzyme, > > immunoreactivity, etc. A couple of weeks in formalin should be fine. > > Paraformaldehyde show be the same as formalin. I do know a way to > restore > > the enzyme activity for TRAP that may have been lost so if u need > > that > let > > me know. > > > > --------- Original Message --------- Subject: AW: [Histonet] Bone > > samples long-term storage in 10% formalin or 4% paraformaldehyde > > From: "Gudrun Lang" > > Date: 12/5/13 11:42 am > > To: "'Orla M Gallagher'" > > Cc: histonet@lists.utsouthwestern.edu > > > > Paraformaldehyd is formaldehyd in solid form. Formalin is the > > aequous solution of formaldehyd. > > So the main characteristics are the same. > > > > Gudrun Lang > > > > -----Ursprüngliche Nachricht----- > > Von: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von > > Orla > M > > Gallagher > > Gesendet: Donnerstag, 05. Dezember 2013 19:31 > > An: histonet@lists.utsouthwestern.edu > > Betreff: [Histonet] Bone samples long-term storage in 10% formalin > > or 4% paraformaldehyde > > > > Dear Histonetters, > > > > What is your opinion on storing bone samples long-term (more than a > > couple of weeks) in 10% formalin? As I was taught, best practice > > has always been to fix only as long as necessary, depending on the > > size of > the > > sample, then decalcify and process to wax, and I always stress this > > to everyone I advise. > > > > However, research colleagues sometimes wish to do histology on bone > > samples that have been stored for months ..or even years! As the > formalin > > pH becomes more acidic, there is formalin pigment and the > immunoreactivity > > and TRAP enzyme activity is diminished or destroyed during long > fixation, > > is there any way of minimising this e.g. has anyone tried regularly > > replacing the old formalin with fresh buffered formalin, or storing > > formalin-fixed bones in any other medium? I'm also interested in > > how > best > > to fix in 4% paraformaldehyde and whether the problems are the same > > with long-term storage. > > > > Thanks for your comments. > > > > All the best, > > Orla > > > > -- > > ************************** > > Ms. Orla Gallagher > > Bone Analysis Laboratory > > Mellanby Centre for Bone Research > > Department of Human Metabolism > > D Floor Medical School > > University of Sheffield > > Beech Hill Road > > Sheffield > > S10 2RX > > UK > > > > Website: http://mellanbycentre.dept.shef.ac.uk > > > > Tel: 0044114-2713337 (office) > > 0044114-2713174 (lab) > > E-Mail: o.m.gallagher@sheffield.ac.uk > > > > > > *STOP*: Do you really need to print this e-mail? > > > > *BE GREEN:* Keep it on the screen. > > > > > > *Times Higher Education University of the Year* > > > > > > > > Data protection and confidentiality: > > The information contained in this message or any appended documents > > may be privileged and confidential and is intended for the > > exclusive use of the addressee(s). If you are not the addressee, > > any disclosure, reproduction, distributions, other dissemination or > > use of this message > is > > strictly prohibited and may be unlawful. If you receive this > > correspondence in error please contact the sender immediately and > > permanently delete/destroy what you have received. > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > -- > ************************** > Ms. Orla Gallagher > Bone Analysis Laboratory > Mellanby Centre for Bone Research > Department of Human Metabolism > D Floor Medical School > University of Sheffield > Beech Hill Road > Sheffield > S10 2RX > UK > > Website: http://mellanbycentre.dept.shef.ac.uk > > Tel: 0044114-2713337 (office) > 0044114-2713174 (lab) > E-Mail: o.m.gallagher@sheffield.ac.uk > > > *STOP*: Do you really need to print this e-mail? > > *BE GREEN:* Keep it on the screen. > > > *Times Higher Education University of the Year* > > > > Data protection and confidentiality: > The information contained in this message or any appended documents > may be privileged and confidential and is intended for the exclusive > use of the addressee(s). If you are not the addressee, any disclosure, > reproduction, distributions, other dissemination or use of this > message is strictly prohibited and may be unlawful. If you receive > this correspondence in error please contact the sender immediately and > permanently delete/destroy what you have received. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From smcbride <@t> andrew.cmu.edu Mon Dec 9 10:46:24 2013 From: smcbride <@t> andrew.cmu.edu (Sean McBride) Date: Mon Dec 9 10:46:35 2013 Subject: [Histonet] Bone samples long-term storage in 10% formalin or 4% paraformaldehyde In-Reply-To: References: <000001cef1e9$c9d5a960$5d80fc20$@gmx.at> <20131205134938.f86bd30e73b823f57b516b5451216a98.fb3f94f1f4.mailapi@email01.secureserver.net> <1EB8F245A303564EADF12AC7022FA74D70F43E28@NOVUS-EX01.novusint.com> Message-ID: <3B509E4A-DAAE-494E-B66F-885719AC0D21@andrew.cmu.edu> Dear Orla, Post fixation, we have stored our bone specimens in 1x PBS while having them sent out for MicroCT analysis. We have also stored them in 1x PBS at 4?C post fixation when necessary until further processing without having adverse affects on our staining. However, we chose conventional staining over IHC for our results. Best regards, ~Sean McBride Scientific Specialist Bone Tissue Engineering Center Carnegie Mellon Research Institute Suite 4311 700 Technology Drive Pittsburgh, PA 15219-3124 412-268-8275 (o) 412-915-1683 (m) 412-268-8275 (fax) smcbride@andrew.cmu.edu On Dec 9, 2013, at 8:44 AM, Orla M Gallagher wrote: > Thanks to everyone for your comments. > > I may not have been clear in my question - our researchers don't wish to > decalcify these formalin-fixed bones yet, but rather to store them for more > than a couple of weeks, in case they need to carry out MicroCT followed by > histology later. I'm aware that the formalin or paraformaldehyde will > degrade over time, but I just wondered if anyone has a protocol for storage > without decalcification? I guess transfer to 70% ethanol is an option but > this is also not ideal for longterm storage, and would need to be removed > before decal in EDTA. > > All the best, > Orla > > > On 6 December 2013 16:12, Wineman, Terra wrote: > >> I would suggest a different protocol if the tissue will not be processed >> for a while. I would say a week in 10%NBF and then transfer the bones to >> an EDTA decal solution. The bones will decal slowly without the affects of >> the formic acid. I am in research and this is what we do with our bones. >> >> Terra Wineman, HTL (ASCP)CM >> Research Biologist >> 636-926-7476 phone >> terra.wineman@novusint.com >> >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu [mailto: >> histonet-bounces@lists.utsouthwestern.edu] On Behalf Of pruegg@ihctech.net >> Sent: Thursday, December 05, 2013 2:50 PM >> To: gu.lang@gmx.at; 'Orla M Gallagher' >> Cc: histonet@lists.utsouthwestern.edu >> Subject: RE: AW: [Histonet] Bone samples long-term storage in 10% formalin >> or 4% paraformaldehyde >> >> i would think u are correct in advising formic acid decal and then >> processing into paraffin for the best protection of the trap enzyme, >> immunoreactivity, etc. A couple of weeks in formalin should be fine. >> Paraformaldehyde show be the same as formalin. I do know a way to restore >> the enzyme activity for TRAP that may have been lost so if u need that let >> me know. >> >> --------- Original Message --------- Subject: AW: [Histonet] Bone samples >> long-term storage in 10% formalin or 4% paraformaldehyde >> From: "Gudrun Lang" >> Date: 12/5/13 11:42 am >> To: "'Orla M Gallagher'" >> Cc: histonet@lists.utsouthwestern.edu >> >> Paraformaldehyd is formaldehyd in solid form. Formalin is the aequous >> solution of formaldehyd. >> So the main characteristics are the same. >> >> Gudrun Lang >> >> -----Ursprüngliche Nachricht----- >> Von: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Orla M >> Gallagher >> Gesendet: Donnerstag, 05. Dezember 2013 19:31 >> An: histonet@lists.utsouthwestern.edu >> Betreff: [Histonet] Bone samples long-term storage in 10% formalin or 4% >> paraformaldehyde >> >> Dear Histonetters, >> >> What is your opinion on storing bone samples long-term (more than a >> couple of weeks) in 10% formalin? As I was taught, best practice has >> always been to fix only as long as necessary, depending on the size of the >> sample, then decalcify and process to wax, and I always stress this to >> everyone I advise. >> >> However, research colleagues sometimes wish to do histology on bone >> samples that have been stored for months ..or even years! As the formalin >> pH becomes more acidic, there is formalin pigment and the immunoreactivity >> and TRAP enzyme activity is diminished or destroyed during long fixation, >> is there any way of minimising this e.g. has anyone tried regularly >> replacing the old formalin with fresh buffered formalin, or storing >> formalin-fixed bones in any other medium? I'm also interested in how best >> to fix in 4% paraformaldehyde and whether the problems are the same with >> long-term storage. >> >> Thanks for your comments. >> >> All the best, >> Orla >> >> -- >> ************************** >> Ms. Orla Gallagher >> Bone Analysis Laboratory >> Mellanby Centre for Bone Research >> Department of Human Metabolism >> D Floor Medical School >> University of Sheffield >> Beech Hill Road >> Sheffield >> S10 2RX >> UK >> >> Website: http://mellanbycentre.dept.shef.ac.uk >> >> Tel: 0044114-2713337 (office) >> 0044114-2713174 (lab) >> E-Mail: o.m.gallagher@sheffield.ac.uk >> >> >> *STOP*: Do you really need to print this e-mail? >> >> *BE GREEN:* Keep it on the screen. >> >> >> *Times Higher Education University of the Year* >> >> >> >> Data protection and confidentiality: >> The information contained in this message or any appended documents may >> be privileged and confidential and is intended for the exclusive use of >> the addressee(s). If you are not the addressee, any disclosure, >> reproduction, distributions, other dissemination or use of this message is >> strictly prohibited and may be unlawful. If you receive this >> correspondence in error please contact the sender immediately and >> permanently delete/destroy what you have received. >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > -- > ************************** > Ms. Orla Gallagher > Bone Analysis Laboratory > Mellanby Centre for Bone Research > Department of Human Metabolism > D Floor Medical School > University of Sheffield > Beech Hill Road > Sheffield > S10 2RX > UK > > Website: http://mellanbycentre.dept.shef.ac.uk > > Tel: 0044114-2713337 (office) > 0044114-2713174 (lab) > E-Mail: o.m.gallagher@sheffield.ac.uk > > > *STOP*: Do you really need to print this e-mail? > > *BE GREEN:* Keep it on the screen. > > > *Times Higher Education University of the Year* > > > > Data protection and confidentiality: > The information contained in this message or any appended documents may be > privileged and confidential and is intended for the exclusive use of the > addressee(s). If you are not the addressee, any disclosure, reproduction, > distributions, other dissemination or use of this message is strictly > prohibited and may be unlawful. If you receive this correspondence in error > please contact the sender immediately and permanently delete/destroy what > you have received. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jamie.erickson <@t> abbvie.com Mon Dec 9 13:06:27 2013 From: jamie.erickson <@t> abbvie.com (Erickson, Jamie E) Date: Mon Dec 9 13:06:33 2013 Subject: [Histonet] CD4 and CD8 mouse tumor IHC, thanks.. Message-ID: <8B946A68A8F3534A99CC493DEFB49B101763CADD@WM10005P.oneabbott.com> Thank you to those that sent me suggestion on my CD4 problem. I have recently discovered the problem. The tumors are mouse tumors but they are injected in-vivo with a test antibody that is Rat anti-mouse, specifically Isotype (Rat IgG2a). Hence this is why my secondary Rabbit anti-rat stained so much.. Next I will try to block the Rat in-vivo injected antibody with a goat anti-Rat secondary, as a block prior to staining with CD4. Or I could precomplex the CD4 with the Rabbit Anti-Rat and bind excess Anti-Rat with Rat Serum. Any thoughts or suggestion on this as always would be welcomed... Thanks again.. Jamie Abbvie Bioresearch Center Pharmacology 100 Research Dr. Worcester, Ma 01605 OFFICE +1 508-688-3134 FAX +1 508-793-4895 EMAIL jamie.erickson@abbvie.com From gayle.callis <@t> bresnan.net Mon Dec 9 13:57:03 2013 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Mon Dec 9 13:57:24 2013 Subject: [Histonet] CD4 and CD8 mouse tumor IHC, thanks.. In-Reply-To: <8B946A68A8F3534A99CC493DEFB49B101763CADD@WM10005P.oneabbott.com> References: <8B946A68A8F3534A99CC493DEFB49B101763CADD@WM10005P.oneabbott.com> Message-ID: <000401cef518$d6692bc0$833b8340$@bresnan.net> Another solution, and one we did almost exclusively for murine T and B cell IHC was use biotinylated Rat anti mouse CD4 and CD8. The isotype can be purchased biotinylated from BD Bioscience/Invitrogen or you can buy Jacksons Rat IgG-biotin. This totally eliminates a secondary antibody hence anything rat will not be detected. A bonus is staining is much faster too. We routinely did Streptavidin/biotin block from Vector since Streptavidin has an affinity for integrins in epithelial cells. Our normal serum block with biotinylated rat antimouse primaries is 10% goat or donkey serum + 2.5% mouse. The block is also the diluent for the primary antibody and isotype/IgG negative control. Rabbit serum and antibodies were avoided since the bunny is sticky, and can cause more background. My immunologist frowned on anything using a rabbit host unless we couldn't avoid it. Good luck Gayle Callis HTL/HT/MT(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Erickson, Jamie E Sent: Monday, December 09, 2013 12:06 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CD4 and CD8 mouse tumor IHC, thanks.. Thank you to those that sent me suggestion on my CD4 problem. I have recently discovered the problem. The tumors are mouse tumors but they are injected in-vivo with a test antibody that is Rat anti-mouse, specifically Isotype (Rat IgG2a). Hence this is why my secondary Rabbit anti-rat stained so much.. Next I will try to block the Rat in-vivo injected antibody with a goat anti-Rat secondary, as a block prior to staining with CD4. Or I could precomplex the CD4 with the Rabbit Anti-Rat and bind excess Anti-Rat with Rat Serum. Any thoughts or suggestion on this as always would be welcomed... Thanks again.. Jamie Abbvie Bioresearch Center Pharmacology 100 Research Dr. Worcester, Ma 01605 OFFICE +1 508-688-3134 FAX +1 508-793-4895 EMAIL jamie.erickson@abbvie.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rheyna <@t> lumc.edu Mon Dec 9 14:47:06 2013 From: rheyna <@t> lumc.edu (Roger Heyna) Date: Mon Dec 9 14:47:18 2013 Subject: [Histonet] Discontinuing Negative Reagent Controls for IHC Message-ID: <52A5D7EA020000230008957A@gwgwia1.luhs.org> I am putting together a list of facilities that have discontinued the use of negative reagent controls for IHC. After the CAP revised the requirement related to negative controls when polymer-based detection systems are used, we decided to investigate whether discontinuing the negative controls would be possible for our lab. It would be helpful to know what labs have done this successfully. If the labs that have discontinued the use of negatives could just respond in an email, I would appreciate it. Also, if anyone has any thoughts pertaining to this change, they're certainly welcome. Thank you, Roger Maywood, IL From Richard.Cartun <@t> hhchealth.org Mon Dec 9 15:58:30 2013 From: Richard.Cartun <@t> hhchealth.org (Cartun, Richard) Date: Mon Dec 9 15:58:36 2013 Subject: [Histonet] Discontinuing Negative Reagent Controls for IHC In-Reply-To: <52A5D7EA020000230008957A@gwgwia1.luhs.org> References: <52A5D7EA020000230008957A@gwgwia1.luhs.org> Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E0189DF48@HHCEXCHMB05.hhcsystem.org> "Hartford Hospital", Hartford, CT Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax richard.cartun@hhchealth.org ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Roger Heyna [rheyna@lumc.edu] Sent: Monday, December 09, 2013 3:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Discontinuing Negative Reagent Controls for IHC I am putting together a list of facilities that have discontinued the use of negative reagent controls for IHC. After the CAP revised the requirement related to negative controls when polymer-based detection systems are used, we decided to investigate whether discontinuing the negative controls would be possible for our lab. It would be helpful to know what labs have done this successfully. If the labs that have discontinued the use of negatives could just respond in an email, I would appreciate it. Also, if anyone has any thoughts pertaining to this change, they're certainly welcome. Thank you, Roger Maywood, IL This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From melissa <@t> alliedsearchpartners.com Mon Dec 9 16:03:06 2013 From: melissa <@t> alliedsearchpartners.com (Melissa Phelan) Date: Mon Dec 9 16:04:02 2013 Subject: [Histonet] ASCP Certified Pathologist Assistant Job in Nebraska (Perm or Temp to Perm) Message-ID: Hello, I have a Full Time/Permanent Pathologists Assistant position available in the Omaha, NE area. This position requires ASCP certification as a Pathologist Assistant. I am ideally looking for someone for a long term/permanent position however, a temp to perm option may be available. Relocation assistance available if permanent position is accepted. Please send resume if interested. Thank you, To view a complete list of Allied Search Partners current openings go to: http://www.alliedsearchpartners.com/careers.php -- Melissa Phelan President, Laboratory Staffing Allied Search Partners www.linkedin.com/in/melissaphelan/ http://www.alliedsearchpartners.com T: 888.388.7571 ext. 102 F: 888.388.7572 *If you wish to no longer receive emails from Allied Search Partners please respond to this email message with "remove." This email including its attachments is intended only for the confidential use of the individual to whom it is addressed. If you are not the intended recipient, any use, dissemination, distribution or copying of this message or its attachments is prohibited. If you have received this message in error, please notify us immediately, and delete this message and its attachments permanently from your system. From SHUNTER <@t> beaumont.edu Tue Dec 10 06:50:26 2013 From: SHUNTER <@t> beaumont.edu (Sue Hunter) Date: Tue Dec 10 06:50:35 2013 Subject: [Histonet] Discontinuing Negative Reagent Controls for IHC In-Reply-To: <9215BD4B0BA1B44D962A71C758B68D2E0189DF48@HHCEXCHMB05.hhcsystem.org> References: <52A5D7EA020000230008957A@gwgwia1.luhs.org> <9215BD4B0BA1B44D962A71C758B68D2E0189DF48@HHCEXCHMB05.hhcsystem.org> Message-ID: Beaumont Hospital, Royal Oak MI. It's great! Sue Sue Hunter, Supervisor Advanced Diagnostics Beaumont Health System Royal Oak MI 248-898-5146 shunter@beaumont.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cartun, Richard Sent: Monday, December 09, 2013 4:59 PM To: Roger Heyna; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Discontinuing Negative Reagent Controls for IHC "Hartford Hospital", Hartford, CT Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax richard.cartun@hhchealth.org ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Roger Heyna [rheyna@lumc.edu] Sent: Monday, December 09, 2013 3:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Discontinuing Negative Reagent Controls for IHC I am putting together a list of facilities that have discontinued the use of negative reagent controls for IHC. After the CAP revised the requirement related to negative controls when polymer-based detection systems are used, we decided to investigate whether discontinuing the negative controls would be possible for our lab. It would be helpful to know what labs have done this successfully. If the labs that have discontinued the use of negatives could just respond in an email, I would appreciate it. Also, if anyone has any thoughts pertaining to this change, they're certainly welcome. Thank you, Roger Maywood, IL This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SHUNTER <@t> beaumont.edu Tue Dec 10 06:51:36 2013 From: SHUNTER <@t> beaumont.edu (Sue Hunter) Date: Tue Dec 10 06:51:40 2013 Subject: [Histonet] Decon disinfectant for Ultras Message-ID: Has anyone used a disinfectant other than Lysol IC to do the decon on the Ventana Ultras? Thanks Sue Sue Hunter, Supervisor Advanced Diagnostics Beaumont Health System Royal Oak MI 248-898-5146 shunter@beaumont.edu From mturner <@t> CSILaboratories.com Tue Dec 10 07:32:17 2013 From: mturner <@t> CSILaboratories.com (Mark Turner) Date: Tue Dec 10 07:32:24 2013 Subject: [Histonet] Discontinuing Negative Reagent Controls for IHC In-Reply-To: References: <52A5D7EA020000230008957A@gwgwia1.luhs.org> <9215BD4B0BA1B44D962A71C758B68D2E0189DF48@HHCEXCHMB05.hhcsystem.org> Message-ID: <643626B74DE2814D8537057F40E1A10B046BBAB2@CSI-MX-NODEA.CSI-LABS.local> Here at CSI Labs we stopped negative controls for routine assays last August upon verification of the new standard and it has not been a problem. We do, however, continue to validate using negatives to ensure good quality. Mark Turner, Ph.D., HT(ASCP)QIHC Manager, Histology/IHC CSI Laboratories Alpharetta, GA ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sue Hunter Sent: Tuesday, December 10, 2013 7:50 AM To: Cartun, Richard; Roger Heyna; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Discontinuing Negative Reagent Controls for IHC Beaumont Hospital, Royal Oak MI. It's great! Sue Sue Hunter, Supervisor Advanced Diagnostics Beaumont Health System Royal Oak MI 248-898-5146 shunter@beaumont.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cartun, Richard Sent: Monday, December 09, 2013 4:59 PM To: Roger Heyna; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Discontinuing Negative Reagent Controls for IHC "Hartford Hospital", Hartford, CT Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax richard.cartun@hhchealth.org ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Roger Heyna [rheyna@lumc.edu] Sent: Monday, December 09, 2013 3:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Discontinuing Negative Reagent Controls for IHC I am putting together a list of facilities that have discontinued the use of negative reagent controls for IHC. After the CAP revised the requirement related to negative controls when polymer-based detection systems are used, we decided to investigate whether discontinuing the negative controls would be possible for our lab. It would be helpful to know what labs have done this successfully. If the labs that have discontinued the use of negatives could just respond in an email, I would appreciate it. Also, if anyone has any thoughts pertaining to this change, they're certainly welcome. Thank you, Roger Maywood, IL This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LSebree <@t> uwhealth.org Tue Dec 10 07:32:27 2013 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Tue Dec 10 07:32:33 2013 Subject: [Histonet] RE: Decon disinfectant for Ultras In-Reply-To: References: Message-ID: <77DD817201982748BC67D7960F2F76AF07381C@UWHC-MBX12.uwhis.hosp.wisc.edu> Sue, I believe we used Amphyll at one point when we couldn't get Lysol IC. But that could have been before we had our Ultras so used it on XT and Benchmark. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sue Hunter Sent: Tuesday, December 10, 2013 6:52 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Decon disinfectant for Ultras Has anyone used a disinfectant other than Lysol IC to do the decon on the Ventana Ultras? Thanks Sue Sue Hunter, Supervisor Advanced Diagnostics Beaumont Health System Royal Oak MI 248-898-5146 shunter@beaumont.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From richard.wild <@t> wanadoo.fr Tue Dec 10 09:34:37 2013 From: richard.wild <@t> wanadoo.fr (richard wild) Date: Tue Dec 10 09:34:55 2013 Subject: [Histonet] pathcentre spare reagent bottles (looking for) In-Reply-To: <3b57dd61339e9d290a2c2ad700b00465@mwinf5c38.me-wanadoo.net> References: <3b57dd61339e9d290a2c2ad700b00465@mwinf5c38.me-wanadoo.net> Message-ID: <52A7348D.6090107@wanadoo.fr> Hello I am looking for one or two (at least) spare reagent bottles for Shandon Pathcentre. Does anyone have these bottle to sell or to give ? Best regards Richard From mhale <@t> MiracaLS.com Tue Dec 10 10:41:09 2013 From: mhale <@t> MiracaLS.com (Hale, Meredith) Date: Tue Dec 10 10:41:15 2013 Subject: [Histonet] California HT Position Message-ID: <0E828EC51C7CC445A51E53F81B64E8C73A494E@s-irv-exchmb.PathologyPartners.intranet> Great Full time opportunity for Histotechnician's in Yuba City, California ! North Valley Gastroenterology is looking for certified HT's or HTL's to join their laboratory . Candidate must meet the following criteria: * Meet CLIA Grossing Requirements : CFR 493.1489, http://wwwn.cdc.gov/clia/regs/toc.aspx/ ,prior experience grossing GI specimens * HT ASCP Certified Duties include: * Grossing * Embedding * Microtomy * Staining * Ability to be flexible and take on additional duties' as needed This is a full time position that offers a competitive rate and flexible hours. Interested applicants should contact Meredith Hale; phone 214-596-2219 or through email mhale@miracals.com Meredith Hale HT (ASCP)cm Director External Sales Support Miraca Life Sciences 6655 North MacArthur Blvd. Irving , Texas 75039 Office: 214-596-2219 Cell: 469-648-8253 Fax: 1-866-688-3280 mhale@miracals.com> From joewalker <@t> rrmc.org Tue Dec 10 11:31:47 2013 From: joewalker <@t> rrmc.org (Joe W. Walker, Jr.) Date: Tue Dec 10 11:31:52 2013 Subject: [Histonet] Discontinuing Negative Reagent Controls for IHC In-Reply-To: <643626B74DE2814D8537057F40E1A10B046BBAB2@CSI-MX-NODEA.CSI-LABS.local> References: <52A5D7EA020000230008957A@gwgwia1.luhs.org> <9215BD4B0BA1B44D962A71C758B68D2E0189DF48@HHCEXCHMB05.hhcsystem.org> <643626B74DE2814D8537057F40E1A10B046BBAB2@CSI-MX-NODEA.CSI-LABS.local> Message-ID: <3C2378778400AD448ADA6FD6BDB7CCCC1815F949@RRMBX03.rrmc.local> We too have discontinued using negative controls but do utilize them when working up new antibodies. Joe W. Walker, Jr. MS, SCT(ASCP)CM Manager of Anatomical Pathology, Microbiology and Reference Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P: 802.747.1790 F: 802.747.6525 Email joewalker@rrmc.org www.rrmc.org Our Vision: To be the Best Community Healthcare System in New England Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet Recognition? and the Governor's Award for Performance Excellence -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Turner Sent: Tuesday, December 10, 2013 8:32 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Discontinuing Negative Reagent Controls for IHC Here at CSI Labs we stopped negative controls for routine assays last August upon verification of the new standard and it has not been a problem. We do, however, continue to validate using negatives to ensure good quality. Mark Turner, Ph.D., HT(ASCP)QIHC Manager, Histology/IHC CSI Laboratories Alpharetta, GA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sue Hunter Sent: Tuesday, December 10, 2013 7:50 AM To: Cartun, Richard; Roger Heyna; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Discontinuing Negative Reagent Controls for IHC Beaumont Hospital, Royal Oak MI. It's great! Sue Sue Hunter, Supervisor Advanced Diagnostics Beaumont Health System Royal Oak MI 248-898-5146 shunter@beaumont.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cartun, Richard Sent: Monday, December 09, 2013 4:59 PM To: Roger Heyna; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Discontinuing Negative Reagent Controls for IHC "Hartford Hospital", Hartford, CT Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax richard.cartun@hhchealth.org ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Roger Heyna [rheyna@lumc.edu] Sent: Monday, December 09, 2013 3:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Discontinuing Negative Reagent Controls for IHC I am putting together a list of facilities that have discontinued the use of negative reagent controls for IHC. After the CAP revised the requirement related to negative controls when polymer-based detection systems are used, we decided to investigate whether discontinuing the negative controls would be possible for our lab. It would be helpful to know what labs have done this successfully. If the labs that have discontinued the use of negatives could just respond in an email, I would appreciate it. Also, if anyone has any thoughts pertaining to this change, they're certainly welcome. Thank you, Roger Maywood, IL This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. Thank You From Donna.Millard <@t> prlnet.com Tue Dec 10 13:42:54 2013 From: Donna.Millard <@t> prlnet.com (Donna Millard) Date: Tue Dec 10 13:43:02 2013 Subject: [Histonet] Discontinuing Negative Reagent Controls for IHC Message-ID: <64D11CD058311340A1966C234C6B0B891220E609@PRLEXCH02.prlnet.com> We happily discontinued use of negative controls a year ago, and it was a non-issue on CAP inspection. We do continue to run them on stains for Image, as that system was FDA approved and validated using a negative control. Donna Millard Director of Anatomic Pathology Physicians Reference Laboratory, LLC 7800 W. 110th Street,Overland Park, KS 66210 Direct: 913-339-0485 Fax: 913-319-4156 CONFIDENTIALITY NOTICE This message and any included attachments are from Physicians Reference Laboratory, LLC and are intended only for the addressee.The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call PRL's corporate offices in Overland Park, Kansas, U.S.A at (913)338-4070 From AHutton <@t> dh.org Tue Dec 10 13:52:40 2013 From: AHutton <@t> dh.org (Hutton, Allison) Date: Tue Dec 10 13:52:46 2013 Subject: [Histonet] TMA for ER and PR In-Reply-To: <64D11CD058311340A1966C234C6B0B891220E609@PRLEXCH02.prlnet.com> References: <64D11CD058311340A1966C234C6B0B891220E609@PRLEXCH02.prlnet.com> Message-ID: <4ED8C96A8F20FC4F883A92E2A0A0D64AEBDB@DH-MAIL01.dhorg.org> We are in the process of validating ER and PR and are not having much luck. My pathologist was curious if any companies happen to sell control slides for these antibodies that contain cores of each score. (He actually is looking for something similiar to the CAP proficiency slides). Any direction would be appreciated. Also, if anyone has any protocols they are willing to share, we are using the Leica Bond-Max and Leica RTU ER and PR antibodies. Thanks Allison From Timothy.Morken <@t> ucsfmedctr.org Tue Dec 10 14:25:20 2013 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Tue Dec 10 14:29:48 2013 Subject: [Histonet] RE: TMA for ER and PR In-Reply-To: <4ED8C96A8F20FC4F883A92E2A0A0D64AEBDB@DH-MAIL01.dhorg.org> References: <64D11CD058311340A1966C234C6B0B891220E609@PRLEXCH02.prlnet.com> <4ED8C96A8F20FC4F883A92E2A0A0D64AEBDB@DH-MAIL01.dhorg.org> Message-ID: <761E2B5697F795489C8710BCC72141FF0B7079@ex07.net.ucsf.edu> Allison, Pantomics has such an array. See this page: http://www.pantomics.com/tissueArrays.aspx?nature=IHC Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hutton, Allison Sent: Tuesday, December 10, 2013 11:53 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] TMA for ER and PR We are in the process of validating ER and PR and are not having much luck. My pathologist was curious if any companies happen to sell control slides for these antibodies that contain cores of each score. (He actually is looking for something similiar to the CAP proficiency slides). Any direction would be appreciated. Also, if anyone has any protocols they are willing to share, we are using the Leica Bond-Max and Leica RTU ER and PR antibodies. Thanks Allison _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From schaundrawalton <@t> yahoo.com Tue Dec 10 15:22:32 2013 From: schaundrawalton <@t> yahoo.com (Schaundra Walton) Date: Tue Dec 10 15:22:38 2013 Subject: [Histonet] Eosinophil specific IHC for Mouse Message-ID: <1386710552.74879.YahooMailNeo@web122006.mail.ne1.yahoo.com> A collegue of mine is looking for an eosinophil specific antibody that would work in mouse tissue.? Does anybody have any insight or recommendations? ? Thanks, Schaundra Walton BS HTL(ASCP) From Richard.Cartun <@t> hhchealth.org Tue Dec 10 15:33:56 2013 From: Richard.Cartun <@t> hhchealth.org (Cartun, Richard) Date: Tue Dec 10 15:34:02 2013 Subject: [Histonet] 2014 IHC CPT coding Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E0189EB5A@HHCEXCHMB05.hhcsystem.org> My understanding is that 88342 is used for the first IHC antibody, then 88343 is used for each subsequent antibody (per specimen part). Is that correct? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax richard.cartun@hhchealth.org This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From Joyce.Weems <@t> emoryhealthcare.org Tue Dec 10 15:40:14 2013 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Tue Dec 10 15:40:26 2013 Subject: [Histonet] RE: 2014 IHC CPT coding In-Reply-To: <9215BD4B0BA1B44D962A71C758B68D2E0189EB5A@HHCEXCHMB05.hhcsystem.org> References: <9215BD4B0BA1B44D962A71C758B68D2E0189EB5A@HHCEXCHMB05.hhcsystem.org> Message-ID: And there are different codes for Medicare which is going to be a mess. We can't bill on order because we will have to know if the patient is Medicare if I understand it correctly. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cartun, Richard Sent: Tuesday, December 10, 2013 4:34 PM To: Histonet Subject: [Histonet] 2014 IHC CPT coding My understanding is that 88342 is used for the first IHC antibody, then 88343 is used for each subsequent antibody (per specimen part). Is that correct? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax richard.cartun@hhchealth.org This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From lblazek <@t> digestivespecialists.com Tue Dec 10 15:45:35 2013 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Tue Dec 10 15:45:39 2013 Subject: [Histonet] RE: 2014 IHC CPT coding In-Reply-To: <9215BD4B0BA1B44D962A71C758B68D2E0189EB5A@HHCEXCHMB05.hhcsystem.org> References: <9215BD4B0BA1B44D962A71C758B68D2E0189EB5A@HHCEXCHMB05.hhcsystem.org> Message-ID: <5A2BD13465E061429D6455C8D6B40E3916760A0E04@IBMB7Exchange.digestivespecialists.com> 88342 for the first IHC antibody then 88343 for subsequent antibodies on the SAME SLIDE is my understanding. Linda -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cartun, Richard Sent: Tuesday, December 10, 2013 4:34 PM To: Histonet Subject: [Histonet] 2014 IHC CPT coding My understanding is that 88342 is used for the first IHC antibody, then 88343 is used for each subsequent antibody (per specimen part). Is that correct? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax richard.cartun@hhchealth.org This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From one_angel_secret <@t> yahoo.com Tue Dec 10 16:22:34 2013 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Tue Dec 10 16:22:46 2013 Subject: [Histonet] 2014 IHC CPT coding In-Reply-To: <9215BD4B0BA1B44D962A71C758B68D2E0189EB5A@HHCEXCHMB05.hhcsystem.org> References: <9215BD4B0BA1B44D962A71C758B68D2E0189EB5A@HHCEXCHMB05.hhcsystem.org> Message-ID: <9E1ED0CE-4C23-4FFB-B983-CDE984ABD796@yahoo.com> I got information they got rid of 88342 all together and added G codes. One for the 1st slide stained and another for each additional stain. Can anyone verify and give a link? Sent from my iPhone On Dec 10, 2013, at 4:33 PM, "Cartun, Richard" wrote: > My understanding is that 88342 is used for the first IHC antibody, then 88343 is used for each subsequent antibody (per specimen part). Is that correct? Thank you. > > > > Richard > > > > Richard W. Cartun, MS, PhD > > Director, Histology & Immunopathology > > Director, Biospecimen Collection Programs > > Assistant Director, Anatomic Pathology > > Hartford Hospital > > 80 Seymour Street > > Hartford, CT 06102 > > (860) 545-1596 Office > > (860) 545-2204 Fax > > richard.cartun@hhchealth.org > > This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From 41dmb41 <@t> gmail.com Tue Dec 10 16:34:40 2013 From: 41dmb41 <@t> gmail.com (Drew Meyer) Date: Tue Dec 10 16:35:07 2013 Subject: [Histonet] 2014 IHC CPT coding In-Reply-To: <9E1ED0CE-4C23-4FFB-B983-CDE984ABD796@yahoo.com> References: <9215BD4B0BA1B44D962A71C758B68D2E0189EB5A@HHCEXCHMB05.hhcsystem.org> <9E1ED0CE-4C23-4FFB-B983-CDE984ABD796@yahoo.com> Message-ID: This is from the CAP site... and here's the link: http://www.cap.org/apps/cap.portal?_nfpb=true&cntvwrPtlt_actionOverride=/portlets/contentViewer/show&_windowLabel=cntvwrPtlt&cntvwrPtlt%7BactionForm.contentReference%7D=statline/index.html&_state=maximized&_pageLabel=cntvwr *Immunohistochemistry: 88342 (PC & TC)* CMS rejected the CAP?s proposal and instead will require the use of two new G codes for this service, including G0461 to report one unit of service per specimen and G0462 to report each additional stain. The following chart documents the percentage reduction and payment rate change for the new G codes compared to the current reimbursement for CPT code 88342: *CPT Code**Modifier* *88342 2013 Total Payment**2014 Total Payment* *Total % Change from 2013 88342* G0461 $115.34 $88.04-24% G0461TC$73.15 $57.39-22%G0461 26$42.19 $30.65-27% G0462 $115.34 $68.08-41% G0462TC$73.15 $55.61-24% G0462 26$42.19 $12.48-70% The changes occurred under the Affordable Care Act (ACA), which provided CMS expanded authority to launch its ?misvalued code? initiative. CMS targeted the top expenditure codes from each specialty as potentially overvalued. This action triggered the review of the three additional high volume code families. Moving forward, the CAP will work with the AMA and other groups to pressure CMS to change their G code definitions so that payment is made ?per slide? rather than ?per specimen.? On Tue, Dec 10, 2013 at 5:22 PM, Kim Donadio wrote: > I got information they got rid of 88342 all together and added G codes. > One for the 1st slide stained and another for each additional stain. Can > anyone verify and give a link? > > Sent from my iPhone > > On Dec 10, 2013, at 4:33 PM, "Cartun, Richard" < > Richard.Cartun@hhchealth.org> wrote: > > > My understanding is that 88342 is used for the first IHC antibody, then > 88343 is used for each subsequent antibody (per specimen part). Is that > correct? Thank you. > > > > > > > > Richard > > > > > > > > Richard W. Cartun, MS, PhD > > > > Director, Histology & Immunopathology > > > > Director, Biospecimen Collection Programs > > > > Assistant Director, Anatomic Pathology > > > > Hartford Hospital > > > > 80 Seymour Street > > > > Hartford, CT 06102 > > > > (860) 545-1596 Office > > > > (860) 545-2204 Fax > > > > richard.cartun@hhchealth.org > > > > This e-mail message, including any attachments, is for the sole use of > the intended recipient(s) and may contain confidential and privileged > information. Any unauthorized review, use, disclosure, or distribution is > prohibited. If you are not the intended recipient, or an employee or agent > responsible for delivering the message to the intended recipient, please > contact the sender by reply e-mail and destroy all copies of the original > message, including any attachments. > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From foreightl <@t> gmail.com Tue Dec 10 16:42:21 2013 From: foreightl <@t> gmail.com (Patrick Laurie) Date: Tue Dec 10 16:42:25 2013 Subject: [Histonet] 2014 IHC CPT coding In-Reply-To: References: <9215BD4B0BA1B44D962A71C758B68D2E0189EB5A@HHCEXCHMB05.hhcsystem.org> <9E1ED0CE-4C23-4FFB-B983-CDE984ABD796@yahoo.com> Message-ID: Yes, we are trying to wrap our heads around this one. 24% drop for the first IHC stain done on a specimen and 41% for each one following. This will hit our IHC department hard. Perhaps even will hurt patient care. At least the 88360 came out unscathed. Patrick Laurie Celligent Diagnostics On Tue, Dec 10, 2013 at 5:34 PM, Drew Meyer <41dmb41@gmail.com> wrote: > This is from the CAP site... and here's the link: > > > http://www.cap.org/apps/cap.portal?_nfpb=true&cntvwrPtlt_actionOverride=/portlets/contentViewer/show&_windowLabel=cntvwrPtlt&cntvwrPtlt%7BactionForm.contentReference%7D=statline/index.html&_state=maximized&_pageLabel=cntvwr > > *Immunohistochemistry: 88342 (PC & TC)* > CMS rejected the CAP?s proposal and instead will require the use of two new > G codes for this service, including G0461 to report one unit of service per > specimen and G0462 to report each additional stain. The following chart > documents the percentage reduction and payment rate change for the new G > codes compared to the current reimbursement for CPT code 88342: > *CPT Code**Modifier* *88342 2013 Total Payment**2014 Total Payment* *Total > % Change from 2013 88342* G0461 $115.34 $88.04-24% G0461TC$73.15 > $57.39-22%G0461 > 26$42.19 $30.65-27% G0462 $115.34 $68.08-41% G0462TC$73.15 $55.61-24% G0462 > 26$42.19 $12.48-70% > > The changes occurred under the Affordable Care Act (ACA), which provided > CMS expanded authority to launch its ?misvalued code? initiative. CMS > targeted the top expenditure codes from each specialty as potentially > overvalued. This action triggered the review of the three additional high > volume code families. > > Moving forward, the CAP will work with the AMA and other groups to pressure > CMS to change their G code definitions so that payment is made ?per slide? > rather than ?per specimen.? > > > On Tue, Dec 10, 2013 at 5:22 PM, Kim Donadio >wrote: > > > I got information they got rid of 88342 all together and added G codes. > > One for the 1st slide stained and another for each additional stain. Can > > anyone verify and give a link? > > > > Sent from my iPhone > > > > On Dec 10, 2013, at 4:33 PM, "Cartun, Richard" < > > Richard.Cartun@hhchealth.org> wrote: > > > > > My understanding is that 88342 is used for the first IHC antibody, then > > 88343 is used for each subsequent antibody (per specimen part). Is that > > correct? Thank you. > > > > > > > > > > > > Richard > > > > > > > > > > > > Richard W. Cartun, MS, PhD > > > > > > Director, Histology & Immunopathology > > > > > > Director, Biospecimen Collection Programs > > > > > > Assistant Director, Anatomic Pathology > > > > > > Hartford Hospital > > > > > > 80 Seymour Street > > > > > > Hartford, CT 06102 > > > > > > (860) 545-1596 Office > > > > > > (860) 545-2204 Fax > > > > > > richard.cartun@hhchealth.org > > > > > > This e-mail message, including any attachments, is for the sole use of > > the intended recipient(s) and may contain confidential and privileged > > information. Any unauthorized review, use, disclosure, or distribution is > > prohibited. If you are not the intended recipient, or an employee or > agent > > responsible for delivering the message to the intended recipient, please > > contact the sender by reply e-mail and destroy all copies of the original > > message, including any attachments. > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie(HT)ASCP QIHC Histology Manager Celligent Diagnostics, LLC 101 East W.T. Harris Blvd | Suite 1212 | Charlotte, NC 28262 Work: 704-970-3300 Cell: 704-266-0869 From Sheila.Tapper <@t> EssentiaHealth.org Tue Dec 10 17:01:30 2013 From: Sheila.Tapper <@t> EssentiaHealth.org (Tapper, Sheila J.) Date: Tue Dec 10 17:01:41 2013 Subject: [Histonet] 2014 IHC CPT coding In-Reply-To: References: <9215BD4B0BA1B44D962A71C758B68D2E0189EB5A@HHCEXCHMB05.hhcsystem.org><9E1ED0CE-4C23-4FFB-B983-CDE984ABD796@yahoo.com> Message-ID: <2A99E5980E5E0345B542E681A9A3F978065E9332@grouse.medcampus.org> Anyone doing Ventana's Dual ISH will take a big hit... you will not be able to apply two 88368 codes to the same slide - so a 50% reduction there... ___________________________________________ Sheila Tapper Anatomic Pathology Supervisor Essentia Health SMDC Clinical Laboratory | SMMC Pathology 3W 407 East Third Street, Duluth, MN 55805 P: 218-786-5472 | F: 218-786-2369 sheila.tapper@essentiahealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patrick Laurie Sent: Tuesday, December 10, 2013 4:42 PM To: Drew Meyer Cc: Histonet; Cartun,Richard Subject: Re: [Histonet] 2014 IHC CPT coding Yes, we are trying to wrap our heads around this one. 24% drop for the first IHC stain done on a specimen and 41% for each one following. This will hit our IHC department hard. Perhaps even will hurt patient care. At least the 88360 came out unscathed. Patrick Laurie Celligent Diagnostics On Tue, Dec 10, 2013 at 5:34 PM, Drew Meyer <41dmb41@gmail.com> wrote: > This is from the CAP site... and here's the link: > > > http://www.cap.org/apps/cap.portal?_nfpb=true&cntvwrPtlt_actionOverride= /portlets/contentViewer/show&_windowLabel=cntvwrPtlt&cntvwrPtlt%7Baction Form.contentReference%7D=statline/index.html&_state=maximized&_pageLabel =cntvwr > > *Immunohistochemistry: 88342 (PC & TC)* > CMS rejected the CAP's proposal and instead will require the use of two new > G codes for this service, including G0461 to report one unit of service per > specimen and G0462 to report each additional stain. The following chart > documents the percentage reduction and payment rate change for the new G > codes compared to the current reimbursement for CPT code 88342: > *CPT Code**Modifier* *88342 2013 Total Payment**2014 Total Payment* *Total > % Change from 2013 88342* G0461 $115.34 $88.04-24% G0461TC$73.15 > $57.39-22%G0461 > 26$42.19 $30.65-27% G0462 $115.34 $68.08-41% G0462TC$73.15 $55.61-24% G0462 > 26$42.19 $12.48-70% > > The changes occurred under the Affordable Care Act (ACA), which provided > CMS expanded authority to launch its "misvalued code" initiative. CMS > targeted the top expenditure codes from each specialty as potentially > overvalued. This action triggered the review of the three additional high > volume code families. > > Moving forward, the CAP will work with the AMA and other groups to pressure > CMS to change their G code definitions so that payment is made 'per slide' > rather than 'per specimen.' > > > On Tue, Dec 10, 2013 at 5:22 PM, Kim Donadio >wrote: > > > I got information they got rid of 88342 all together and added G codes. > > One for the 1st slide stained and another for each additional stain. Can > > anyone verify and give a link? > > > > Sent from my iPhone > > > > On Dec 10, 2013, at 4:33 PM, "Cartun, Richard" < > > Richard.Cartun@hhchealth.org> wrote: > > > > > My understanding is that 88342 is used for the first IHC antibody, then > > 88343 is used for each subsequent antibody (per specimen part). Is that > > correct? Thank you. > > > > > > > > > > > > Richard > > > > > > > > > > > > Richard W. Cartun, MS, PhD > > > > > > Director, Histology & Immunopathology > > > > > > Director, Biospecimen Collection Programs > > > > > > Assistant Director, Anatomic Pathology > > > > > > Hartford Hospital > > > > > > 80 Seymour Street > > > > > > Hartford, CT 06102 > > > > > > (860) 545-1596 Office > > > > > > (860) 545-2204 Fax > > > > > > richard.cartun@hhchealth.org > > > > > > This e-mail message, including any attachments, is for the sole use of > > the intended recipient(s) and may contain confidential and privileged > > information. Any unauthorized review, use, disclosure, or distribution is > > prohibited. If you are not the intended recipient, or an employee or > agent > > responsible for delivering the message to the intended recipient, please > > contact the sender by reply e-mail and destroy all copies of the original > > message, including any attachments. > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie(HT)ASCP QIHC Histology Manager Celligent Diagnostics, LLC 101 East W.T. Harris Blvd | Suite 1212 | Charlotte, NC 28262 Work: 704-970-3300 Cell: 704-266-0869 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. As required by federal and state laws, you need to hold this information as privileged and confidential. If you have received this communication in error, please notify the sender and destroy all copies of this communication and any attachments. From roxannes40 <@t> gmail.com Tue Dec 10 17:03:03 2013 From: roxannes40 <@t> gmail.com (Roxanne) Date: Tue Dec 10 17:03:09 2013 Subject: [Histonet] 2014 CMS/AMA IHC Changes Message-ID: <86AC93B5-6C53-4DDB-A25E-0F1412847DDD@gmail.com> We are incorporating the new rules into our LIS/billing system for 2014. I just to verify how others are interpreting these rules. Medicare - my understanding is that the G codes are used for all IHC, G0461 for the first IHC and G0462 for any additional antibody on the same specimen, regardless if it is a cocktail or not. I think this language is clear G0461 = "per specimen;first single or multiplex antibody" and G0462 = "per specimen; each additional single or multiples antibody" Third Party Payors - AMA changed the wording from specimen to block, to enable billing separate blocks on the same specimen. 88342 = "each separately identifiable antibody per block; first separately identifiable antibody per slide". 88343 = "each additional, separately identifiable antibody per slide". So it is clear that 88343 is for cocktail stains, but the guidelines changed from specimen to block. And the G-codes clearly state specimen, not block and it doesnt matter if it is a cocktail or not. My question is, do you agree with my understanding? Do you think that AMA means to say that a block equates to a specimen? Thanks, Roxanne From mw <@t> personifysearch.com Wed Dec 11 07:34:50 2013 From: mw <@t> personifysearch.com (Matt Ward) Date: Wed Dec 11 07:34:56 2013 Subject: [Histonet] Antibody Technical Sales - Northwest Message-ID: <033301cef675$c4df5420$4e9dfc60$@personifysearch.com> Good Morning, We are currently recruiting for an Antibody Staining Technical Sales Specialist with a world leader in cancer diagnostics. The position will be covering the Pacific Northwest. We are searching for professionals who have a strong IHC/Antibody background. If this sounds like a position you would like to learn more about, please send an updated copy of your resume to, mw@personifysearch.com . Have a wonderful day! Matt Ward Account Executive Personify 5020 Weston Parkway Suite 315 Cary NC 27513 (Tel) 800.875.6188 direct ext 103 (Fax) 919.460.0642 www.personifysearch.com From CObregon <@t> mhs.net Wed Dec 11 09:52:43 2013 From: CObregon <@t> mhs.net (Obregon, Cecilia) Date: Wed Dec 11 09:52:54 2013 Subject: [Histonet] Granzyme B Message-ID: <598BB4A3A92D7F4DAA14C30E4AE70C2F27BC9CB4@MHSEXMB05.mhs.net> Good morning, Is anyone running Granzyme B on Ventana Ultra? Can you recommend a protocol? Let me know. Thank you, Cecilia M. Obregon Memorial Regional Hospital 3501 Johnson Street Hollywood, FL 33021 Ph: 954-265-5317 CONFIDENTIALITY NOTICE: DO NOT FORWARD THIS MESSAGE TO OTHERS WITHOUT PERMISSION OF THE SENDER. This e-mail, including any attachments, may contain confidential or privileged material that is exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, dissemination, copying, or taking any action in reliance on its contents is prohibited. If you have any reason to believe this e-mail was not intended for you, please delete the e-mail and any attachments, and notify the sender immediately. From Nancy_Schmitt <@t> pa-ucl.com Wed Dec 11 11:05:23 2013 From: Nancy_Schmitt <@t> pa-ucl.com (Nancy Schmitt) Date: Wed Dec 11 11:05:29 2013 Subject: [Histonet] wax on the floors Message-ID: <906B4DA90ED1DB4DB6C7E94D7CEE6C368152F823@PEITHA.wad.pa-ucl.com> Happy Hump Day! What is everyone doing to clean floors of paraffin? Does anyone have any great secrets to how the floors are cleaned of the debris? Thanks for any input! Nancy Nancy Schmitt HT, MLT(ASCP) Histology Coordinator United Clinical Laboratories NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From contact <@t> excaliburpathology.com Wed Dec 11 11:16:32 2013 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Wed Dec 11 11:16:36 2013 Subject: [Histonet] wax on the floors In-Reply-To: <906B4DA90ED1DB4DB6C7E94D7CEE6C368152F823@PEITHA.wad.pa-ucl.com> References: <906B4DA90ED1DB4DB6C7E94D7CEE6C368152F823@PEITHA.wad.pa-ucl.com> Message-ID: <1386782192.98734.YahooMailNeo@web5704.biz.mail.ne1.yahoo.com> Bruce floor stripper! ? Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 5830 N Blue Lake Dr. Please note new address! Norman, OK 73069 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ________________________________ From: Nancy Schmitt To: "histonet@lists.utsouthwestern.edu" Sent: Wednesday, December 11, 2013 11:05 AM Subject: [Histonet] wax on the floors Happy Hump Day! What is everyone doing to clean floors of paraffin?? Does anyone have any great secrets to how the floors are cleaned of the debris? Thanks for any input! Nancy Nancy Schmitt HT, MLT(ASCP) Histology Coordinator United Clinical Laboratories NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vtobias <@t> uw.edu Wed Dec 11 11:25:16 2013 From: vtobias <@t> uw.edu (Victor A. Tobias) Date: Wed Dec 11 11:27:25 2013 Subject: [Histonet] wax on the floors In-Reply-To: <1386782192.98734.YahooMailNeo@web5704.biz.mail.ne1.yahoo.com> References: <906B4DA90ED1DB4DB6C7E94D7CEE6C368152F823@PEITHA.wad.pa-ucl.com> <1386782192.98734.YahooMailNeo@web5704.biz.mail.ne1.yahoo.com> Message-ID: Is Bruce a Brand name or the employee? Victor Tobias HT(ASCP) Clinical Applications Analyst Harborview Medical Center Dept of Pathology Room NJB 244 Ninth & Jefferson Seattle, WA 98104 vtobias@u.washington.edu 206-744-2735 206-744-8240 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Wednesday, December 11, 2013 9:17 AM To: Nancy Schmitt; Histonet Subject: Re: [Histonet] wax on the floors Bruce floor stripper! ? Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 5830 N Blue Lake Dr. Please note new address! Norman, OK 73069 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ________________________________ From: Nancy Schmitt To: "histonet@lists.utsouthwestern.edu" Sent: Wednesday, December 11, 2013 11:05 AM Subject: [Histonet] wax on the floors Happy Hump Day! What is everyone doing to clean floors of paraffin?? Does anyone have any great secrets to how the floors are cleaned of the debris? Thanks for any input! Nancy Nancy Schmitt HT, MLT(ASCP) Histology Coordinator United Clinical Laboratories NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Wed Dec 11 11:21:01 2013 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Wed Dec 11 11:29:59 2013 Subject: [Histonet] RE: wax on the floors In-Reply-To: <906B4DA90ED1DB4DB6C7E94D7CEE6C368152F823@PEITHA.wad.pa-ucl.com> References: <906B4DA90ED1DB4DB6C7E94D7CEE6C368152F823@PEITHA.wad.pa-ucl.com> Message-ID: <761E2B5697F795489C8710BCC72141FF0B737D@ex07.net.ucsf.edu> Our environmental services strips and re-waxes the floor at the end of each week. In between it is just sweeping up loose debris. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt Sent: Wednesday, December 11, 2013 9:05 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] wax on the floors Happy Hump Day! What is everyone doing to clean floors of paraffin? Does anyone have any great secrets to how the floors are cleaned of the debris? Thanks for any input! Nancy Nancy Schmitt HT, MLT(ASCP) Histology Coordinator United Clinical Laboratories NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From patrick.lewis <@t> seattlechildrens.org Wed Dec 11 11:35:20 2013 From: patrick.lewis <@t> seattlechildrens.org (Lewis, Patrick) Date: Wed Dec 11 11:35:29 2013 Subject: [Histonet] Best place to buy Copland jars. Message-ID: <3903BE18914F4440834F0E620415FFCA382004A8@PPWEXD01a.childrens.sea.kids> Hi everyone Can someone recommend a good place to buy Copland jars. I want the glass jars with the plastic circle top screw on lid. I only want about 4 of them and cost is an issue. Thanks Patrick. CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From contact <@t> excaliburpathology.com Wed Dec 11 11:49:03 2013 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Wed Dec 11 11:49:08 2013 Subject: [Histonet] wax on the floors In-Reply-To: References: <906B4DA90ED1DB4DB6C7E94D7CEE6C368152F823@PEITHA.wad.pa-ucl.com> <1386782192.98734.YahooMailNeo@web5704.biz.mail.ne1.yahoo.com> Message-ID: <1386784143.76836.YahooMailNeo@web5706.biz.mail.ne1.yahoo.com> A brand name! LOL ? Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 5830 N Blue Lake Dr. Please note new address! Norman, OK 73069 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ________________________________ From: Victor A. Tobias To: Paula Pierce ; Nancy Schmitt ; Histonet Sent: Wednesday, December 11, 2013 11:25 AM Subject: RE: [Histonet] wax on the floors Is Bruce a Brand name or the employee? Victor Tobias HT(ASCP) Clinical Applications Analyst Harborview Medical Center Dept of Pathology Room NJB 244 Ninth & Jefferson Seattle, WA 98104 vtobias@u.washington.edu 206-744-2735 206-744-8240 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Wednesday, December 11, 2013 9:17 AM To: Nancy Schmitt; Histonet Subject: Re: [Histonet] wax on the floors Bruce floor stripper! ? Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 5830 N Blue Lake Dr. Please note new address! Norman, OK 73069 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ________________________________ From: Nancy Schmitt To: "histonet@lists.utsouthwestern.edu" Sent: Wednesday, December 11, 2013 11:05 AM Subject: [Histonet] wax on the floors Happy Hump Day! What is everyone doing to clean floors of paraffin?? Does anyone have any great secrets to how the floors are cleaned of the debris? Thanks for any input! Nancy Nancy Schmitt HT, MLT(ASCP) Histology Coordinator United Clinical Laboratories NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bbrinegarhtl <@t> gmail.com Wed Dec 11 12:17:45 2013 From: bbrinegarhtl <@t> gmail.com (Beth Brinegar) Date: Wed Dec 11 12:17:52 2013 Subject: [Histonet] wax on the floors In-Reply-To: <1386784143.76836.YahooMailNeo@web5706.biz.mail.ne1.yahoo.com> References: <906B4DA90ED1DB4DB6C7E94D7CEE6C368152F823@PEITHA.wad.pa-ucl.com> <1386782192.98734.YahooMailNeo@web5704.biz.mail.ne1.yahoo.com> <1386784143.76836.YahooMailNeo@web5706.biz.mail.ne1.yahoo.com> Message-ID: We use a paraffin scraper - it looks like an oversized ice scraper for your car, however it gets the debris off and in pile so it's easier to sweep it away. Then every couple of months, it's stripped and waxed. Beth Brinegar HTL(ASCP) Anatomic Pathology Supervisor Mercy Medical Center Cedar Rapids, IA 52403 On Wed, Dec 11, 2013 at 11:49 AM, Paula Pierce < contact@excaliburpathology.com> wrote: > A brand name! LOL > > Paula K. Pierce, HTL(ASCP)HT > President > Excalibur Pathology, Inc. > 5830 N Blue Lake Dr. Please note new address! > Norman, OK 73069 > 405-759-3953 Lab > 405-759-7513 Fax > www.excaliburpathology.com > > > ________________________________ > From: Victor A. Tobias > To: Paula Pierce ; Nancy Schmitt < > Nancy_Schmitt@pa-ucl.com>; Histonet > Sent: Wednesday, December 11, 2013 11:25 AM > Subject: RE: [Histonet] wax on the floors > > > Is Bruce a Brand name or the employee? > > Victor Tobias HT(ASCP) > Clinical Applications Analyst > Harborview Medical Center > Dept of Pathology Room NJB 244 > Ninth & Jefferson > Seattle, WA 98104 > vtobias@u.washington.edu > 206-744-2735 > 206-744-8240 Fax > ================================================= > Privileged, confidential or patient identifiable information may be > contained in this message. This information is meant only for the use > of the intended recipients. If you are not the intended recipient, or > if the message has been addressed to you in error, do not read, > disclose, reproduce, distribute, disseminate or otherwise use this > transmission. Instead, please notify the sender by reply e-mail, and > then destroy all copies of the message and any attachments. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Pierce > Sent: Wednesday, December 11, 2013 9:17 AM > To: Nancy Schmitt; Histonet > Subject: Re: [Histonet] wax on the floors > > Bruce floor stripper! > > Paula K. Pierce, HTL(ASCP)HT > President > Excalibur Pathology, Inc. > 5830 N Blue Lake Dr. Please note new address! > Norman, OK 73069 > 405-759-3953 Lab > 405-759-7513 Fax > www.excaliburpathology.com > > > ________________________________ > From: Nancy Schmitt > To: "histonet@lists.utsouthwestern.edu" > > Sent: Wednesday, December 11, 2013 11:05 AM > Subject: [Histonet] wax on the floors > > > Happy Hump Day! > > What is everyone doing to clean floors of paraffin? Does anyone have any > great secrets to how the floors are cleaned of the debris? > > Thanks for any input! > > Nancy > > Nancy Schmitt HT, MLT(ASCP) > Histology Coordinator > United Clinical Laboratories > > > > > NOTICE: This email may contain legally privileged information. The > information > is for the use of only the intended recipient(s) even if addressed > incorrectly. If you are not the intended recipient, please notify the > sender > that you have received it in error and then delete it along with any > attachments. Thank you. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Donna.Millard <@t> prlnet.com Wed Dec 11 13:21:55 2013 From: Donna.Millard <@t> prlnet.com (Donna Millard) Date: Wed Dec 11 13:22:06 2013 Subject: [Histonet] 2014 CMS/AMA IHC Changes Message-ID: <64D11CD058311340A1966C234C6B0B8922D6D146@PRLEXCH02.prlnet.com> Roxanne, I agree with your interpretation, as does the director of our billing. Our coders are going to have fun. We are putting the burden of them to correctly bill / code according to payor, as accessioners and pathologists will not have the relevant information to make that call. Donna Millard Director of Anatomic Pathology Physicians Reference Laboratory, LLC 7800 W. 110th Street,Overland Park, KS 66210 Direct: 913-339-0485 Fax: 913-319-4156 CONFIDENTIALITY NOTICE This message and any included attachments are from Physicians Reference Laboratory, LLC and are intended only for the addressee.The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call PRL's corporate offices in Overland Park, Kansas, U.S.A at (913)338-4070 From TNMayer <@t> mdanderson.org Wed Dec 11 14:16:49 2013 From: TNMayer <@t> mdanderson.org (Mayer,Toysha N) Date: Wed Dec 11 14:17:56 2013 Subject: [Histonet] Re: wax on the floors Message-ID: <47E9B2C01DDDD94881EACD2DC44EBC8802C145F4@D1PWPEXMBX05.mdanderson.edu> Ok, I could not resist. Let's see, I have used: Paint scraper Slide (when I first started) Spackle spatula taped to a broomstick (when I worked at small, poor place) Ice thingy (removes the ice from car windshield) hey I live in Houston, we don't have ice Floor scraper from a carpet/vinyl floor company (when I worked in a fancy lab) Also, don't let housekeeping wax the floors. Sincerely, Toysha N. Mayer, MBA, HT(ASCP) tnmayer@mdanderson.org Instructor/Education Coordinator Program in Histotechnology School of Health Professions MD Anderson Cancer Center 713-563.3481 Message: 1 Date: Wed, 11 Dec 2013 09:49:03 -0800 (PST) From: Paula Pierce Subject: Re: [Histonet] wax on the floors To: "Victor A. Tobias" , Histonet Message-ID: <1386784143.76836.YahooMailNeo@web5706.biz.mail.ne1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 A brand name! LOL ? Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 5830 N Blue Lake Dr. Please note new address! Norman, OK 73069 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ________________________________ From: Victor A. Tobias To: Paula Pierce ; Nancy Schmitt ; Histonet Sent: Wednesday, December 11, 2013 11:25 AM Subject: RE: [Histonet] wax on the floors Is Bruce a Brand name or the employee? Victor Tobias HT(ASCP) Clinical Applications Analyst Harborview Medical Center Dept of Pathology Room NJB 244 Ninth & Jefferson Seattle, WA 98104 vtobias@u.washington.edu 206-744-2735 206-744-8240 Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Wednesday, December 11, 2013 9:17 AM To: Nancy Schmitt; Histonet Subject: Re: [Histonet] wax on the floors Bruce floor stripper! ? Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 5830 N Blue Lake Dr. Please note new address! Norman, OK 73069 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ________________________________ From: Nancy Schmitt To: "histonet@lists.utsouthwestern.edu" Sent: Wednesday, December 11, 2013 11:05 AM Subject: [Histonet] wax on the floors Happy Hump Day! What is everyone doing to clean floors of paraffin?? Does anyone have any great secrets to how the floors are cleaned of the debris? Thanks for any input! Nancy Nancy Schmitt HT, MLT(ASCP) Histology Coordinator United Clinical Laboratories NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 121, Issue 15 ***************************************** From BDeBrosse-Serra <@t> isisph.com Wed Dec 11 14:31:28 2013 From: BDeBrosse-Serra <@t> isisph.com (Bea DeBrosse-Serra) Date: Wed Dec 11 14:31:55 2013 Subject: [Histonet] wax on the floors In-Reply-To: References: <906B4DA90ED1DB4DB6C7E94D7CEE6C368152F823@PEITHA.wad.pa-ucl.com> <1386782192.98734.YahooMailNeo@web5704.biz.mail.ne1.yahoo.com> <1386784143.76836.YahooMailNeo@web5706.biz.mail.ne1.yahoo.com> Message-ID: Paraffin scraper from Mercedes Medical http://www.mercedesmedical.com/default.aspx?page=item+detail&itemcode=MKL+ML1138 Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Beth Brinegar Sent: Wednesday, December 11, 2013 10:18 AM To: Paula Pierce Cc: Histonet Subject: Re: [Histonet] wax on the floors We use a paraffin scraper - it looks like an oversized ice scraper for your car, however it gets the debris off and in pile so it's easier to sweep it away. Then every couple of months, it's stripped and waxed. Beth Brinegar HTL(ASCP) Anatomic Pathology Supervisor Mercy Medical Center Cedar Rapids, IA 52403 On Wed, Dec 11, 2013 at 11:49 AM, Paula Pierce < contact@excaliburpathology.com> wrote: > A brand name! LOL > > Paula K. Pierce, HTL(ASCP)HT > President > Excalibur Pathology, Inc. > 5830 N Blue Lake Dr. Please note new address! > Norman, OK 73069 > 405-759-3953 Lab > 405-759-7513 Fax > www.excaliburpathology.com > > > ________________________________ > From: Victor A. Tobias > To: Paula Pierce ; Nancy Schmitt < > Nancy_Schmitt@pa-ucl.com>; Histonet > > Sent: Wednesday, December 11, 2013 11:25 AM > Subject: RE: [Histonet] wax on the floors > > > Is Bruce a Brand name or the employee? > > Victor Tobias HT(ASCP) > Clinical Applications Analyst > Harborview Medical Center > Dept of Pathology Room NJB 244 > Ninth & Jefferson > Seattle, WA 98104 > vtobias@u.washington.edu > 206-744-2735 > 206-744-8240 Fax > ================================================= > Privileged, confidential or patient identifiable information may be > contained in this message. This information is meant only for the use > of the intended recipients. If you are not the intended recipient, or > if the message has been addressed to you in error, do not read, > disclose, reproduce, distribute, disseminate or otherwise use this > transmission. Instead, please notify the sender by reply e-mail, and > then destroy all copies of the message and any attachments. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Pierce > Sent: Wednesday, December 11, 2013 9:17 AM > To: Nancy Schmitt; Histonet > Subject: Re: [Histonet] wax on the floors > > Bruce floor stripper! > > Paula K. Pierce, HTL(ASCP)HT > President > Excalibur Pathology, Inc. > 5830 N Blue Lake Dr. Please note new address! > Norman, OK 73069 > 405-759-3953 Lab > 405-759-7513 Fax > www.excaliburpathology.com > > > ________________________________ > From: Nancy Schmitt > To: "histonet@lists.utsouthwestern.edu" > > > Sent: Wednesday, December 11, 2013 11:05 AM > Subject: [Histonet] wax on the floors > > > Happy Hump Day! > > What is everyone doing to clean floors of paraffin? Does anyone have > any great secrets to how the floors are cleaned of the debris? > > Thanks for any input! > > Nancy > > Nancy Schmitt HT, MLT(ASCP) > Histology Coordinator > United Clinical Laboratories > > > > > NOTICE: This email may contain legally privileged information. The > information is for the use of only the intended recipient(s) even if > addressed incorrectly. If you are not the intended recipient, please > notify the sender that you have received it in error and then delete > it along with any attachments. Thank you. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek <@t> digestivespecialists.com Wed Dec 11 14:32:41 2013 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Wed Dec 11 14:32:47 2013 Subject: [Histonet] RE: wax on the floors In-Reply-To: <47E9B2C01DDDD94881EACD2DC44EBC8802C145F4@D1PWPEXMBX05.mdanderson.edu> References: <47E9B2C01DDDD94881EACD2DC44EBC8802C145F4@D1PWPEXMBX05.mdanderson.edu> Message-ID: <5A2BD13465E061429D6455C8D6B40E3916760A106E@IBMB7Exchange.digestivespecialists.com> I'm still looking for Bruce the stripper! We use to use a paraffin floor scraper kind of like a scraper on the end of a broom stick but somehow it disappeared. We now use a paraffin scrapper that is much easier on the floor. The one designed for scrapping the floor had a blade that had a tendency to gouge the floor. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mayer,Toysha N Sent: Wednesday, December 11, 2013 3:17 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Re: wax on the floors Ok, I could not resist. Let's see, I have used: Paint scraper Slide (when I first started) Spackle spatula taped to a broomstick (when I worked at small, poor place) Ice thingy (removes the ice from car windshield) hey I live in Houston, we don't have ice Floor scraper from a carpet/vinyl floor company (when I worked in a fancy lab) Also, don't let housekeeping wax the floors. Sincerely, Toysha N. Mayer, MBA, HT(ASCP) tnmayer@mdanderson.org Instructor/Education Coordinator Program in Histotechnology School of Health Professions MD Anderson Cancer Center 713-563.3481 Message: 1 Date: Wed, 11 Dec 2013 09:49:03 -0800 (PST) From: Paula Pierce Subject: Re: [Histonet] wax on the floors To: "Victor A. Tobias" , Histonet Message-ID: <1386784143.76836.YahooMailNeo@web5706.biz.mail.ne1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 A brand name! LOL ? Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 5830 N Blue Lake Dr. Please note new address! Norman, OK 73069 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ________________________________ From: Victor A. Tobias To: Paula Pierce ; Nancy Schmitt ; Histonet Sent: Wednesday, December 11, 2013 11:25 AM Subject: RE: [Histonet] wax on the floors Is Bruce a Brand name or the employee? Victor Tobias HT(ASCP) Clinical Applications Analyst Harborview Medical Center Dept of Pathology Room NJB 244 Ninth & Jefferson Seattle, WA 98104 vtobias@u.washington.edu 206-744-2735 206-744-8240 Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Wednesday, December 11, 2013 9:17 AM To: Nancy Schmitt; Histonet Subject: Re: [Histonet] wax on the floors Bruce floor stripper! ? Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 5830 N Blue Lake Dr. Please note new address! Norman, OK 73069 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ________________________________ From: Nancy Schmitt To: "histonet@lists.utsouthwestern.edu" Sent: Wednesday, December 11, 2013 11:05 AM Subject: [Histonet] wax on the floors Happy Hump Day! What is everyone doing to clean floors of paraffin?? Does anyone have any great secrets to how the floors are cleaned of the debris? Thanks for any input! Nancy Nancy Schmitt HT, MLT(ASCP) Histology Coordinator United Clinical Laboratories NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 121, Issue 15 ***************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From patrick.lewis <@t> seattlechildrens.org Wed Dec 11 15:49:45 2013 From: patrick.lewis <@t> seattlechildrens.org (Lewis, Patrick) Date: Wed Dec 11 15:49:58 2013 Subject: [Histonet] Best place to Buy Slides boxes preferably True North. Message-ID: <3903BE18914F4440834F0E620415FFCA3820053E@PPWEXD01a.childrens.sea.kids> Hi Everyone, Can anyone tell me the cheapest place to get True North Slide boxes (100 slides purple box) VWR has them for about $33.00 EACH. We get a shipping discount with VWR but there has got to be a cheaper supplier. Baring that, can anyone recommend a good 100 slide storage box. Preferably in the $10-20.00 per box range. CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From Timothy.Morken <@t> ucsfmedctr.org Wed Dec 11 17:05:05 2013 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Wed Dec 11 17:05:17 2013 Subject: [Histonet] giant tissue embedding tampers? Message-ID: <761E2B5697F795489C8710BCC72141FF0B865A@ex07.net.ucsf.edu> Does anyone know of a giant tissue embedding tamper for the giant cassettes. For whole-mount prostate. The mold is about 6cm x 4 cm (1-5/8 inch x 2-3/8 inch) Thanks for any info! Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center Box 1656 505 Parnassus Ave San Francisco, CA 94143 USA 415.353.1266 (office) tim.morken@ucsfmedctr.org From CIngles <@t> uwhealth.org Wed Dec 11 17:29:28 2013 From: CIngles <@t> uwhealth.org (Ingles Claire) Date: Wed Dec 11 17:29:32 2013 Subject: [Histonet] OT: RE: wax on the floors Message-ID: When you're finished with him, send Bruce my way!!! Claire ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Blazek, Linda [lblazek@digestivespecialists.com] Sent: Wednesday, December 11, 2013 2:32 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: wax on the floors I'm still looking for Bruce the stripper! We use to use a paraffin floor scraper kind of like a scraper on the end of a broom stick but somehow it disappeared. We now use a paraffin scrapper that is much easier on the floor. The one designed for scrapping the floor had a blade that had a tendency to gouge the floor. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mayer,Toysha N Sent: Wednesday, December 11, 2013 3:17 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Re: wax on the floors Ok, I could not resist. Let's see, I have used: Paint scraper Slide (when I first started) Spackle spatula taped to a broomstick (when I worked at small, poor place) Ice thingy (removes the ice from car windshield) hey I live in Houston, we don't have ice Floor scraper from a carpet/vinyl floor company (when I worked in a fancy lab) Also, don't let housekeeping wax the floors. Sincerely, Toysha N. Mayer, MBA, HT(ASCP) tnmayer@mdanderson.org Instructor/Education Coordinator Program in Histotechnology School of Health Professions MD Anderson Cancer Center 713-563.3481 Message: 1 Date: Wed, 11 Dec 2013 09:49:03 -0800 (PST) From: Paula Pierce Subject: Re: [Histonet] wax on the floors To: "Victor A. Tobias" , Histonet Message-ID: <1386784143.76836.YahooMailNeo@web5706.biz.mail.ne1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 A brand name! LOL ? Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 5830 N Blue Lake Dr. Please note new address! Norman, OK 73069 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ________________________________ From: Victor A. Tobias To: Paula Pierce ; Nancy Schmitt ; Histonet Sent: Wednesday, December 11, 2013 11:25 AM Subject: RE: [Histonet] wax on the floors Is Bruce a Brand name or the employee? Victor Tobias HT(ASCP) Clinical Applications Analyst Harborview Medical Center Dept of Pathology Room NJB 244 Ninth & Jefferson Seattle, WA 98104 vtobias@u.washington.edu 206-744-2735 206-744-8240 Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Wednesday, December 11, 2013 9:17 AM To: Nancy Schmitt; Histonet Subject: Re: [Histonet] wax on the floors Bruce floor stripper! ? Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 5830 N Blue Lake Dr. Please note new address! Norman, OK 73069 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ________________________________ From: Nancy Schmitt To: "histonet@lists.utsouthwestern.edu" Sent: Wednesday, December 11, 2013 11:05 AM Subject: [Histonet] wax on the floors Happy Hump Day! What is everyone doing to clean floors of paraffin?? Does anyone have any great secrets to how the floors are cleaned of the debris? Thanks for any input! Nancy Nancy Schmitt HT, MLT(ASCP) Histology Coordinator United Clinical Laboratories NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 121, Issue 15 ***************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hmarlatt26 <@t> gmail.com Wed Dec 11 17:52:54 2013 From: hmarlatt26 <@t> gmail.com (Heather Marlatt) Date: Wed Dec 11 17:53:00 2013 Subject: [Histonet] Plant Pathology Message-ID: Do any of you knowledgeable Histo-folks work with plants? I have a researcher that wants to use FISH probes on some plant samples and I have never prepared plant tissues. If anyone out there has some tips/protocols/recommendations I would really appreciate it. Thank you! From lblazek <@t> digestivespecialists.com Wed Dec 11 18:14:23 2013 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Wed Dec 11 18:14:31 2013 Subject: [Histonet] OT: RE: wax on the floors In-Reply-To: References: Message-ID: <03FB209E-02B6-46A2-B87E-A21EB331185F@digestivespecialists.com> I'm sure there won't be much left when we're finished. Sent from my iPhone On Dec 11, 2013, at 6:31 PM, "Ingles Claire" wrote: > When you're finished with him, send Bruce my way!!! > Claire > > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Blazek, Linda [lblazek@digestivespecialists.com] > Sent: Wednesday, December 11, 2013 2:32 PM > To: 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] RE: wax on the floors > > I'm still looking for Bruce the stripper! > We use to use a paraffin floor scraper kind of like a scraper on the end of a broom stick but somehow it disappeared. We now use a paraffin scrapper that is much easier on the floor. The one designed for scrapping the floor had a blade that had a tendency to gouge the floor. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mayer,Toysha N > Sent: Wednesday, December 11, 2013 3:17 PM > To: 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] Re: wax on the floors > > Ok, I could not resist. Let's see, I have used: > Paint scraper > Slide (when I first started) > Spackle spatula taped to a broomstick (when I worked at small, poor place) Ice thingy (removes the ice from car windshield) hey I live in Houston, we don't have ice Floor scraper from a carpet/vinyl floor company (when I worked in a fancy lab) > > Also, don't let housekeeping wax the floors. > > > > Sincerely, > > Toysha N. Mayer, MBA, HT(ASCP) > tnmayer@mdanderson.org > Instructor/Education Coordinator > Program in Histotechnology > School of Health Professions > MD Anderson Cancer Center > 713-563.3481 > > > Message: 1 > Date: Wed, 11 Dec 2013 09:49:03 -0800 (PST) > From: Paula Pierce > Subject: Re: [Histonet] wax on the floors > To: "Victor A. Tobias" , Histonet > > Message-ID: > <1386784143.76836.YahooMailNeo@web5706.biz.mail.ne1.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > A brand name! LOL > ? > Paula K. Pierce, HTL(ASCP)HT > President > Excalibur Pathology, Inc. > 5830 N Blue Lake Dr. Please note new address! > Norman, OK 73069 > 405-759-3953 Lab > 405-759-7513 Fax > www.excaliburpathology.com > > > ________________________________ > From: Victor A. Tobias > To: Paula Pierce ; Nancy Schmitt ; Histonet > Sent: Wednesday, December 11, 2013 11:25 AM > Subject: RE: [Histonet] wax on the floors > > > Is Bruce a Brand name or the employee? > > Victor Tobias HT(ASCP) > Clinical Applications Analyst > Harborview Medical Center > Dept of Pathology Room NJB 244 > Ninth & Jefferson > Seattle, WA 98104 > vtobias@u.washington.edu > 206-744-2735 > 206-744-8240 Fax > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Pierce > Sent: Wednesday, December 11, 2013 9:17 AM > To: Nancy Schmitt; Histonet > Subject: Re: [Histonet] wax on the floors > > Bruce floor stripper! > ? > Paula K. Pierce, HTL(ASCP)HT > President > Excalibur Pathology, Inc. > 5830 N Blue Lake Dr. Please note new address! > Norman, OK 73069 > 405-759-3953 Lab > 405-759-7513 Fax > www.excaliburpathology.com > > > ________________________________ > From: Nancy Schmitt > To: "histonet@lists.utsouthwestern.edu" > Sent: Wednesday, December 11, 2013 11:05 AM > Subject: [Histonet] wax on the floors > > > Happy Hump Day! > > What is everyone doing to clean floors of paraffin?? Does anyone have any great secrets to how the floors are cleaned of the debris? > > Thanks for any input! > > Nancy > > Nancy Schmitt HT, MLT(ASCP) > Histology Coordinator > United Clinical Laboratories > > > > > NOTICE: This email may contain legally privileged information. The information > is for the use of only the intended recipient(s) even if addressed > incorrectly. If you are not the intended recipient, please notify the sender > that you have received it in error and then delete it along with any > attachments. Thank you. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 121, Issue 15 > ***************************************** > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From greyhoundallie <@t> yahoo.com Wed Dec 11 18:53:49 2013 From: greyhoundallie <@t> yahoo.com (Alison Romano) Date: Wed Dec 11 18:53:51 2013 Subject: [Histonet] Filtering stains Message-ID: <000f01cef6d4$a01c1e20$e0545a60$@yahoo.com> Hi! I wondered if anyone has ever heard that filtering stains weakens them. If so, which stains? We do a stain here called a "Combination M" stain, which uses Mallory's, and we do not filter it. Alison Romano HTL-ASCP, VWR International/Ward's Scientific, Henrietta, New York From liz <@t> premierlab.com Wed Dec 11 21:02:24 2013 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Wed Dec 11 21:04:29 2013 Subject: [Histonet] RE: giant tissue embedding tampers? In-Reply-To: <761E2B5697F795489C8710BCC72141FF0B865A@ex07.net.ucsf.edu> References: <761E2B5697F795489C8710BCC72141FF0B865A@ex07.net.ucsf.edu> Message-ID: <14E2C6176416974295479C64A11CB9AE019C79E01F82@SBS2K8.premierlab.local> Tim I bet you might be able to find something in the hardware store that would work for this. A metal bracking or something like that Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 881-0763 cell (303) 682-9060 fax liz@premierlab.com Ship to address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy [Timothy.Morken@ucsfmedctr.org] Sent: Wednesday, December 11, 2013 4:05 PM To: Histonet Subject: [Histonet] giant tissue embedding tampers? Does anyone know of a giant tissue embedding tamper for the giant cassettes. For whole-mount prostate. The mold is about 6cm x 4 cm (1-5/8 inch x 2-3/8 inch) Thanks for any info! Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center Box 1656 505 Parnassus Ave San Francisco, CA 94143 USA 415.353.1266 (office) tim.morken@ucsfmedctr.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Lee.Bond <@t> sclhs.net Thu Dec 12 08:29:57 2013 From: Lee.Bond <@t> sclhs.net (Bond, Lee) Date: Thu Dec 12 08:30:02 2013 Subject: [Histonet] looking for a product Message-ID: Hi All- Can anyone tell me where I can purchase histology base molds in a long, thin rectangular shape? We would like to be able to get more cuts of a prostate biopsy onto a single slide. We currently are using this shape of a base mold and having to manually trim excess wax (dangerous and time consuming): _________________________ / / / ****************** / / * * / / * * / / * * / / * * / / * * / / ****************** / /_______________________/ We would like to find base molds shaped like this, so that we eliminate the need to manually trim each block: _________________________ / / / / / / / ****************** / / * * / / ****************** / / / / / /_______________________/ My staff would be incredibly appreciative for any help in finding this product! Thanks all- Lee Bond, MT(ASCP) SFHC Lab Topeka, KS From pbourassa <@t> karospharma.com Thu Dec 12 08:43:47 2013 From: pbourassa <@t> karospharma.com (Patricia Bourassa) Date: Thu Dec 12 08:43:57 2013 Subject: [Histonet] Erythrocyte Staining - Fixed Tissue Message-ID: Hello! I'm looking for a protocol for specific staining of erythrocytes in paraformaldehyde fixed, paraffin embedded tissue. Is there anything out there other than H&E? Thanks for any help! -- *Patti BourassaSenior Scientist Karos Pharmaceuticals(203) 535-0540, ext 207 <%28203%29%20535-0540%2C%20ext%20207>* From contact <@t> excaliburpathology.com Thu Dec 12 09:02:07 2013 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Thu Dec 12 09:02:12 2013 Subject: [Histonet] Erythrocyte Staining - Fixed Tissue In-Reply-To: References: Message-ID: <1386860527.90972.YahooMailNeo@web5705.biz.mail.ne1.yahoo.com> Treosin from StatLab Medical. RBCs stain a bright orange. ? Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 5830 N Blue Lake Dr. Please note new address! Norman, OK 73069 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ________________________________ From: Patricia Bourassa To: histonet@lists.utsouthwestern.edu Sent: Thursday, December 12, 2013 8:43 AM Subject: [Histonet] Erythrocyte Staining - Fixed Tissue Hello! I'm looking for a protocol for specific staining of erythrocytes in paraformaldehyde fixed, paraffin embedded tissue.? Is there anything out there other than H&E? Thanks for any help! -- *Patti BourassaSenior Scientist Karos Pharmaceuticals(203) 535-0540, ext 207 <%28203%29%20535-0540%2C%20ext%20207>* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From algranth <@t> email.arizona.edu Thu Dec 12 09:02:43 2013 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Thu Dec 12 09:02:48 2013 Subject: [Histonet] RE: giant tissue embedding tampers? In-Reply-To: <14E2C6176416974295479C64A11CB9AE019C79E01F82@SBS2K8.premierlab.local> References: <761E2B5697F795489C8710BCC72141FF0B865A@ex07.net.ucsf.edu> <14E2C6176416974295479C64A11CB9AE019C79E01F82@SBS2K8.premierlab.local> Message-ID: <7D286982-F6E4-4295-B1C3-EEDA0FA813CA@email.arizona.edu> I agree with Liz - or a kitchen store. Whatever kind of store I bet you can make one pretty cheap. I have had our biomed guys here make some things for me for just a few dollars. Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 From katelin09htl <@t> gmail.com Thu Dec 12 09:05:20 2013 From: katelin09htl <@t> gmail.com (Katelin Lester) Date: Thu Dec 12 09:05:26 2013 Subject: [Histonet] RE: wax on the floors In-Reply-To: <5A2BD13465E061429D6455C8D6B40E3916760A106E@IBMB7Exchange.digestivespecialists.com> References: <47E9B2C01DDDD94881EACD2DC44EBC8802C145F4@D1PWPEXMBX05.mdanderson.edu> <5A2BD13465E061429D6455C8D6B40E3916760A106E@IBMB7Exchange.digestivespecialists.com> Message-ID: The kind with the blade was damaging our floors and the blade would break causing a major safety issue. We just bought a long handled scraper from Bel Art; it works really well. http://www.amazon.com/Bel-Art-Scienceware-368290001-Handled-Scraper/dp/B002VBW4N2/ref=sr_1_fkmr0_1?ie=UTF8&qid=1383939732&sr=8-1-fkmr0&keywords=BEL+F368290001+Discrp%3A+Nylon+Long+Handled+Scraper+60%22 Katelin, HTL On Dec 11, 2013 12:32 PM, "Blazek, Linda" wrote: > I'm still looking for Bruce the stripper! > We use to use a paraffin floor scraper kind of like a scraper on the end > of a broom stick but somehow it disappeared. We now use a paraffin > scrapper that is much easier on the floor. The one designed for scrapping > the floor had a blade that had a tendency to gouge the floor. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mayer,Toysha N > Sent: Wednesday, December 11, 2013 3:17 PM > To: 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] Re: wax on the floors > > Ok, I could not resist. Let's see, I have used: > Paint scraper > Slide (when I first started) > Spackle spatula taped to a broomstick (when I worked at small, poor place) > Ice thingy (removes the ice from car windshield) hey I live in Houston, we > don't have ice Floor scraper from a carpet/vinyl floor company (when I > worked in a fancy lab) > > Also, don't let housekeeping wax the floors. > > > > Sincerely, > > Toysha N. Mayer, MBA, HT(ASCP) > tnmayer@mdanderson.org > Instructor/Education Coordinator > Program in Histotechnology > School of Health Professions > MD Anderson Cancer Center > 713-563.3481 > > > Message: 1 > Date: Wed, 11 Dec 2013 09:49:03 -0800 (PST) > From: Paula Pierce > Subject: Re: [Histonet] wax on the floors > To: "Victor A. Tobias" , Histonet > > Message-ID: > <1386784143.76836.YahooMailNeo@web5706.biz.mail.ne1.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > A brand name! LOL > ? > Paula K. Pierce, HTL(ASCP)HT > President > Excalibur Pathology, Inc. > 5830 N Blue Lake Dr. Please note new address! > Norman, OK 73069 > 405-759-3953 Lab > 405-759-7513 Fax > www.excaliburpathology.com > > > ________________________________ > From: Victor A. Tobias > To: Paula Pierce ; Nancy Schmitt < > Nancy_Schmitt@pa-ucl.com>; Histonet > Sent: Wednesday, December 11, 2013 11:25 AM > Subject: RE: [Histonet] wax on the floors > > > Is Bruce a Brand name or the employee? > > Victor Tobias HT(ASCP) > Clinical Applications Analyst > Harborview Medical Center > Dept of Pathology Room NJB 244 > Ninth & Jefferson > Seattle, WA 98104 > vtobias@u.washington.edu > 206-744-2735 > 206-744-8240 Fax > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Pierce > Sent: Wednesday, December 11, 2013 9:17 AM > To: Nancy Schmitt; Histonet > Subject: Re: [Histonet] wax on the floors > > Bruce floor stripper! > ? > Paula K. Pierce, HTL(ASCP)HT > President > Excalibur Pathology, Inc. > 5830 N Blue Lake Dr. Please note new address! > Norman, OK 73069 > 405-759-3953 Lab > 405-759-7513 Fax > www.excaliburpathology.com > > > ________________________________ > From: Nancy Schmitt > To: "histonet@lists.utsouthwestern.edu" > > Sent: Wednesday, December 11, 2013 11:05 AM > Subject: [Histonet] wax on the floors > > > Happy Hump Day! > > What is everyone doing to clean floors of paraffin?? Does anyone have any > great secrets to how the floors are cleaned of the debris? > > Thanks for any input! > > Nancy > > Nancy Schmitt HT, MLT(ASCP) > Histology Coordinator > United Clinical Laboratories > > > > > NOTICE: This email may contain legally privileged information. The > information > is for the use of only the intended recipient(s) even if addressed > incorrectly. If you are not the intended recipient, please notify the > sender > that you have received it in error and then delete it along with any > attachments. Thank you. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 121, Issue 15 > ***************************************** > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From algranth <@t> email.arizona.edu Thu Dec 12 09:18:58 2013 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Thu Dec 12 09:19:03 2013 Subject: [Histonet] looking for a product In-Reply-To: References: Message-ID: <12774A43-5D48-4E7F-A605-77CED8B08941@email.arizona.edu> Long thin base molds...I don't think such a thing exists but you could make some pretty easily. Be innovative! Look around you and see what already exists out there that would work. Recycle! I've found plastic containers from packaged foods and items from hardware stores that could have uses in the lab. There are long thin ice cube trays for ice cubes to put in bottles - could they work for you? Or if you can't find anything, draw up what you want and go to your shop guys. If you come up with something that works well for you I bet there are others who could benefit. We all do the same things but sometimes we have a special twist on what we do and you just can't find everything in a catalog. Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 From algranth <@t> email.arizona.edu Thu Dec 12 09:24:57 2013 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Thu Dec 12 09:25:04 2013 Subject: [Histonet] RE: wax on the floors In-Reply-To: References: <47E9B2C01DDDD94881EACD2DC44EBC8802C145F4@D1PWPEXMBX05.mdanderson.edu> <5A2BD13465E061429D6455C8D6B40E3916760A106E@IBMB7Exchange.digestivespecialists.com> Message-ID: <003906C0-49B9-48D3-91A9-7D9CBE6E9390@email.arizona.edu> Speaking of wax on the floors - they did my floors last nite and they are so pretty I don't think I can drag all the stuff back in that room and go to work. They look like glass but are not slippery. Don't know what they used. Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 From tony.auge <@t> gmail.com Thu Dec 12 09:30:55 2013 From: tony.auge <@t> gmail.com (Tony Auge) Date: Thu Dec 12 09:31:04 2013 Subject: [Histonet] RE: wax on the floors In-Reply-To: <003906C0-49B9-48D3-91A9-7D9CBE6E9390@email.arizona.edu> References: <47E9B2C01DDDD94881EACD2DC44EBC8802C145F4@D1PWPEXMBX05.mdanderson.edu> <5A2BD13465E061429D6455C8D6B40E3916760A106E@IBMB7Exchange.digestivespecialists.com> <003906C0-49B9-48D3-91A9-7D9CBE6E9390@email.arizona.edu> Message-ID: Goo-gone with a green scrubbie on the tough spots. Then alcohol to clean up the goo-gone. -- Tony Auge HTL (ASCP) QIHC Histology Supervisor - Chandler Pathology Services Cell: (651) 373-4768 Email: tony.auge@gmail.com From TGoins <@t> mt.gov Thu Dec 12 09:41:42 2013 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Thu Dec 12 09:41:58 2013 Subject: [Histonet] RE: looking for a product In-Reply-To: References: Message-ID: If you don't want to use a knife blade to trim the excess wax, embed at one end of the mold and use the hot plate to remove excess wax on the other end. Only problem may be getting a ribbon if one edge of the trim surface is not straight. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bond, Lee Sent: Thursday, December 12, 2013 7:30 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] looking for a product Hi All- Can anyone tell me where I can purchase histology base molds in a long, thin rectangular shape? We would like to be able to get more cuts of a prostate biopsy onto a single slide. We currently are using this shape of a base mold and having to manually trim excess wax (dangerous and time consuming): _________________________ / / / ****************** / / * * / / * * / / * * / / * * / / * * / / ****************** / /_______________________/ We would like to find base molds shaped like this, so that we eliminate the need to manually trim each block: _________________________ / / / / / / / ****************** / / * * / / ****************** / / / / / /_______________________/ My staff would be incredibly appreciative for any help in finding this product! Thanks all- Lee Bond, MT(ASCP) SFHC Lab Topeka, KS _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Thu Dec 12 09:55:00 2013 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Thu Dec 12 09:55:09 2013 Subject: [Histonet] RE: looking for a product In-Reply-To: References: Message-ID: <761E2B5697F795489C8710BCC72141FF0B88A0@ex07.net.ucsf.edu> Lee, we are currently working with a company that is developing a "SmartBx" mold specifically for prostate cores - it works with a "receiver" for the bx at the bx suite which holds the bx in a linear trough. Then that "receiver" is put into an embedding mold with corresponding troughs for perfect alignment and flat embedding. And it is sized for minimal trimming. It still needs some refinement but will probably be available in a few months. One caveat...it seems it has to be used with the company's prostate Bx system and may not work as well with other systems. They have a website, but only very minimal information right now. http://www.uc-care.com/products/smartbx Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bond, Lee Sent: Thursday, December 12, 2013 6:30 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] looking for a product Hi All- Can anyone tell me where I can purchase histology base molds in a long, thin rectangular shape? We would like to be able to get more cuts of a prostate biopsy onto a single slide. We currently are using this shape of a base mold and having to manually trim excess wax (dangerous and time consuming): _________________________ / / / ****************** / / * * / / * * / / * * / / * * / / * * / / ****************** / /_______________________/ We would like to find base molds shaped like this, so that we eliminate the need to manually trim each block: _________________________ / / / / / / / ****************** / / * * / / ****************** / / / / / /_______________________/ My staff would be incredibly appreciative for any help in finding this product! Thanks all- Lee Bond, MT(ASCP) SFHC Lab Topeka, KS _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BGapinski <@t> pathgroup.com Thu Dec 12 10:35:56 2013 From: BGapinski <@t> pathgroup.com (Bruce Gapinski) Date: Thu Dec 12 10:36:11 2013 Subject: [Histonet] Re: wax on the floors Message-ID: I beg your pardon!?! Just joking. Bruce is a brand name. And I don't strip. (Certainly not at my age) Respectfully, Bruce Gapinski HT (ASCP) Chief Histologist Marin Medical Laboratories PathGroup SF (415) 209-6076 ________________________________ Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you From JMacDonald <@t> mtsac.edu Thu Dec 12 10:41:48 2013 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Thu Dec 12 10:42:06 2013 Subject: [Histonet] Best place to Buy Slides boxes preferably True North. In-Reply-To: <3903BE18914F4440834F0E620415FFCA3820053E@PPWEXD01a.childrens.sea.kids> Message-ID: Contact Russ at StatLab. We bought ours from StatLab. Good quality latches compared to some we bought. Russell Komae StatLab Medical Products Southern California Account Manager 407 Interchange Street | McKinney, TX 75071 t: 310.529.4465 | f: 972.436.1369 rkomae@statlab.com | www.statlab.com > On Dec 11, 2013, at 1:52 PM, "Lewis, Patrick" wrote: > > Hi Everyone, > > Can anyone tell me the cheapest place to get True North Slide boxes (100 slides purple box) > > VWR has them for about $33.00 EACH. > > We get a shipping discount with VWR but there has got to be a cheaper supplier. > > Baring that, can anyone recommend a good 100 slide storage box. Preferably in the $10-20.00 per box range. > > > > > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b427297 <@t> aol.com Thu Dec 12 10:47:52 2013 From: b427297 <@t> aol.com (b427297@aol.com) Date: Thu Dec 12 10:47:56 2013 Subject: [Histonet] Rodent eye paraffin microtomy In-Reply-To: References: Message-ID: <8D0C57107E22BB3-8AC-1C94@webmail-d156.sysops.aol.com> I have a study coming up where obtaining perfect lens histology is critical. We do a pretty good job routinely, but since the lens is of interest, I would appreciate any tricks and techniques that can help us improve our paraffin lens histology within intact rodent eyes. Thanks, Jackie O' From BDeBrosse-Serra <@t> isisph.com Thu Dec 12 11:12:50 2013 From: BDeBrosse-Serra <@t> isisph.com (Bea DeBrosse-Serra) Date: Thu Dec 12 11:12:56 2013 Subject: [Histonet] Rodent eye paraffin microtomy In-Reply-To: <8D0C57107E22BB3-8AC-1C94@webmail-d156.sysops.aol.com> References: <8D0C57107E22BB3-8AC-1C94@webmail-d156.sysops.aol.com> Message-ID: Let the eye soak in ice water before cutting for a very long time. Bea Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of b427297@aol.com Sent: Thursday, December 12, 2013 8:48 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Rodent eye paraffin microtomy I have a study coming up where obtaining perfect lens histology is critical. We do a pretty good job routinely, but since the lens is of interest, I would appreciate any tricks and techniques that can help us improve our paraffin lens histology within intact rodent eyes. Thanks, Jackie O' _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From aprilp <@t> associateddermhelena.com Thu Dec 12 11:23:32 2013 From: aprilp <@t> associateddermhelena.com (April P - Assocd) Date: Thu Dec 12 11:24:00 2013 Subject: [Histonet] Cutting issues Message-ID: <001b01cef75e$e2fb4e70$a8f1eb50$@associateddermhelena.com> Need feedback. We are a Derm lab We are having an issue with some small punches and shaves being crushed or parched. It does not happen every day and it is maybe one or two when it is happening. Does anyone have any ideas on what can be causing this issue? From rjbuesa <@t> yahoo.com Thu Dec 12 11:46:12 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Dec 12 11:46:16 2013 Subject: [Histonet] Cutting issues In-Reply-To: <001b01cef75e$e2fb4e70$a8f1eb50$@associateddermhelena.com> References: <001b01cef75e$e2fb4e70$a8f1eb50$@associateddermhelena.com> Message-ID: <1386870372.15685.YahooMailNeo@web163105.mail.bf1.yahoo.com> If similar specimens in the processed batch do not have problems, the problems you are having with those few have to be caused before or during fixation. Perhaps drying before fixing could be a cause. Ren? J. ________________________________ From: April P - Assocd To: histonet@lists.utsouthwestern.edu Sent: Thursday, December 12, 2013 12:23 PM Subject: [Histonet] Cutting issues Need feedback. We are a Derm lab We are having an issue with some small punches and shaves being crushed or parched. It does not happen every day and it is maybe one or two when it is happening. Does anyone have any ideas on what can be causing this issue? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rgrow <@t> bmnet.com Thu Dec 12 11:48:02 2013 From: rgrow <@t> bmnet.com (rgrow@bmnet.com) Date: Thu Dec 12 11:48:04 2013 Subject: [Histonet] Job Opening Soon in East Tennessee Message-ID: To: histonet@lists.utsouthwestern.edu Date: 12/12/2013 Blount Memorial Hospital in Maryville, TN will have a histology technician position opening soon. If you are interested in working for a hospital that cares about its patients, we may be what you are looking for. The histology laboratory has excellent ventilation, and ample workspace. We do approximately 9,000 cases per year -routine surgical and biopsy. General histology experience with certification preferred. Immunohistochemistry experience is a plus. Occasional variance in schedule may be necessary as needed, but no weekends. Must demonstrate competency and successfully complete on-the-job orientation through the histology section of the laboratory. Perform all duties of a Histology Technician and other duties as assigned. Applicants must meet the educational and training requirements necessary for certification by the American Society of Clinical Pathology as a Histology Technician or have experience equal to certification. Salary commiserate with experience. We are located in east Tennessee just minutes from the beautiful Smoky Mountains National Park and experience 4 wonderful seasons! All types of outdoor activities are possible. Maryville is host to the annual Foothills Fall Festival with top name entertainment and crafts, and just 30 minutes from Knoxville's culture events and entertainment, as well as UT football, basketball, baseball, etc. Please respond via email with resume attached to: RGROW@BMNET.COM . Thank you Renee Grow, BA., HT (ASCP) rgrow@bmnet.com Histology Supervisor Blount Memorial Hospital 907 E. Lamar Alexander Pkwy. Maryville, TN 37804-5016 (865) 977-4744 (865) 977-5766 Fax ________________________________ This communication may contain protected health information (PHI) that is legally protected from inappropriate disclosure by the Privacy Standards of the Health Insurance Portability and Accountability Act (HIPAA) and relevant Tennessee Laws. If you are not the intended recipient, please note that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this message in error, you should notify the sender immediately by telephone or by return e-mail and delete this message from your computer. Direct questions to the Blount Memorial Hospital Privacy Officer at 865-977-4675. From BDeBrosse-Serra <@t> isisph.com Thu Dec 12 12:22:19 2013 From: BDeBrosse-Serra <@t> isisph.com (Bea DeBrosse-Serra) Date: Thu Dec 12 12:22:29 2013 Subject: [Histonet] 2x3 automated slide scanners Message-ID: Hi histonetters, Does anyone know about any automated 2x3 slide scanners, besides Leica? We know that Leica has one, but does not make any new ones anymore. Thanks in advance, Bea Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 From liz <@t> premierlab.com Thu Dec 12 12:46:15 2013 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Thu Dec 12 12:46:22 2013 Subject: [Histonet] RE: 2x3 automated slide scanners In-Reply-To: References: Message-ID: <14E2C6176416974295479C64A11CB9AE019C79E04F61@SBS2K8.premierlab.local> Bea Huron Technologies has a large format slide scanner. http://www.huron-technologies.com/ up to 8 x 6 slides. Most older Aperio Scanners such as the Scanscope CS or XT can scan 2x3 slides, you just need the 2x3 slide tray, you cannot batch scan its one slide at a time. They did at one time make a 2x3 scanner I know the Allen Brain Institute has one. We can scan 2x3 slides on our Aperio Scanscope XT. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bea DeBrosse-Serra Sent: Thursday, December 12, 2013 11:22 AM To: 'Histonet@lists.utsouthwestern.edu' Subject: [Histonet] 2x3 automated slide scanners Hi histonetters, Does anyone know about any automated 2x3 slide scanners, besides Leica? We know that Leica has one, but does not make any new ones anymore. Thanks in advance, Bea Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b427297 <@t> aol.com Thu Dec 12 12:46:34 2013 From: b427297 <@t> aol.com (b427297@aol.com) Date: Thu Dec 12 12:46:38 2013 Subject: [Histonet] Cutting issues In-Reply-To: <001b01cef75e$e2fb4e70$a8f1eb50$@associateddermhelena.com> References: <001b01cef75e$e2fb4e70$a8f1eb50$@associateddermhelena.com> Message-ID: <8D0C5819DD0BC2E-1238-29CC@webmail-m152.sysops.aol.com> My educated guess would be that the tissues are being dried out prior to being placed in formalin at time of collection. Surgeons are our biggest histology problems. Jackie O' -----Original Message----- From: April P - Assocd To: histonet Sent: Thu, Dec 12, 2013 11:24 am Subject: [Histonet] Cutting issues Need feedback. We are a Derm lab We are having an issue with some small punches and shaves being crushed or arched. It does not happen every day and it is maybe one or two when it is appening. Does anyone have any ideas on what can be causing this issue? _______________________________________________ istonet mailing list istonet@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet From Richard.Cartun <@t> hhchealth.org Thu Dec 12 12:51:57 2013 From: Richard.Cartun <@t> hhchealth.org (Cartun, Richard) Date: Thu Dec 12 12:52:03 2013 Subject: [Histonet] Freezer for tissue storage Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E018A01C8@HHCEXCHMB05.hhcsystem.org> I need a recommendation for a -80 degree C. freezer for storing tissue specimens. Do you prefer upright vs. chest? Thanks. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax richard.cartun@hhchealth.org This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From king.laurie <@t> marshfieldclinic.org Thu Dec 12 12:57:21 2013 From: king.laurie <@t> marshfieldclinic.org (King, Laurie J) Date: Thu Dec 12 12:57:27 2013 Subject: [Histonet] RE: Freezer for tissue storage In-Reply-To: <9215BD4B0BA1B44D962A71C758B68D2E018A01C8@HHCEXCHMB05.hhcsystem.org> References: <9215BD4B0BA1B44D962A71C758B68D2E018A01C8@HHCEXCHMB05.hhcsystem.org> Message-ID: <7578207839F50248A7A6CD33517295EA4B8A64D5@MCL-EXMB03.mfldclin.org> In my experience the uprights have more frost buildup problems, causing sealing problems. The chest ones I've had in the lab do less of this. Laurie -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cartun, Richard Sent: Thursday, December 12, 2013 12:52 PM To: Histonet Subject: [Histonet] Freezer for tissue storage I need a recommendation for a -80 degree C. freezer for storing tissue specimens. Do you prefer upright vs. chest? Thanks. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax richard.cartun@hhchealth.org This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ The contents of this message may contain private, protected and/or privileged information. If you received this message in error, you should destroy the e-mail message and any attachments or copies, and you are prohibited from retaining, distributing, disclosing or using any information contained within. Please contact the sender and advise of the erroneous delivery by return e-mail or telephone. Thank you for your cooperation. From wbenton <@t> cua.md Thu Dec 12 13:02:26 2013 From: wbenton <@t> cua.md (Walter Benton) Date: Thu Dec 12 13:06:26 2013 Subject: [Histonet] RE: Freezer for tissue storage In-Reply-To: <7578207839F50248A7A6CD33517295EA4B8A64D5@MCL-EXMB03.mfldclin.org> References: <9215BD4B0BA1B44D962A71C758B68D2E018A01C8@HHCEXCHMB05.hhcsystem.org>, <7578207839F50248A7A6CD33517295EA4B8A64D5@MCL-EXMB03.mfldclin.org> Message-ID: <0B8979A204680A42B93A52B486088CD93931ABF14E@CUAEXH1.GCU-MD.local> Revco freezers are very good. I prefer uprights for their ease of use (with racks and seeing the stored samples) and use of doors over stryofoam covers used for chest freezers. Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) ChesapeakeUrology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of King, Laurie J [king.laurie@marshfieldclinic.org] Sent: Thursday, December 12, 2013 1:57 PM To: Cartun, Richard; Histonet Subject: [Histonet] RE: Freezer for tissue storage In my experience the uprights have more frost buildup problems, causing sealing problems. The chest ones I've had in the lab do less of this. Laurie -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cartun, Richard Sent: Thursday, December 12, 2013 12:52 PM To: Histonet Subject: [Histonet] Freezer for tissue storage I need a recommendation for a -80 degree C. freezer for storing tissue specimens. Do you prefer upright vs. chest? Thanks. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax richard.cartun@hhchealth.org This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ The contents of this message may contain private, protected and/or privileged information. If you received this message in error, you should destroy the e-mail message and any attachments or copies, and you are prohibited from retaining, distributing, disclosing or using any information contained within. Please contact the sender and advise of the erroneous delivery by return e-mail or telephone. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From Timothy.Morken <@t> ucsfmedctr.org Thu Dec 12 13:14:27 2013 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Thu Dec 12 13:19:53 2013 Subject: [Histonet] RE: Freezer for tissue storage In-Reply-To: <9215BD4B0BA1B44D962A71C758B68D2E018A01C8@HHCEXCHMB05.hhcsystem.org> References: <9215BD4B0BA1B44D962A71C758B68D2E018A01C8@HHCEXCHMB05.hhcsystem.org> Message-ID: <761E2B5697F795489C8710BCC72141FF0B93DF@ex07.net.ucsf.edu> We use Sanyo and Revco uprights with good success. One key ease of use feature has been the use of front-pull racks for sample box storage. Only one row at a time needs to be pulled out rather than a whole rack. We have them linked to a central monitoring system for temperature and power outage alerts so don't bother with chart recorders (though they have LED temperature display). Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cartun, Richard Sent: Thursday, December 12, 2013 10:52 AM To: Histonet Subject: [Histonet] Freezer for tissue storage I need a recommendation for a -80 degree C. freezer for storing tissue specimens. Do you prefer upright vs. chest? Thanks. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax richard.cartun@hhchealth.org This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b-frederick <@t> northwestern.edu Thu Dec 12 13:24:55 2013 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Thu Dec 12 13:25:01 2013 Subject: [Histonet] RE: Freezer for tissue storage In-Reply-To: <7578207839F50248A7A6CD33517295EA4B8A64D5@MCL-EXMB03.mfldclin.org> References: <9215BD4B0BA1B44D962A71C758B68D2E018A01C8@HHCEXCHMB05.hhcsystem.org> <7578207839F50248A7A6CD33517295EA4B8A64D5@MCL-EXMB03.mfldclin.org> Message-ID: <62C639732D3F274DACED033EBDF6ADAF2F02BDE1@evcspmbx2.ads.northwestern.edu> We use -80 uprights and even with a digital temp readout we have a chart on all of them (about 20). We are a tissue bank as well as doing research. We have what we call a freezer farm. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of King, Laurie J Sent: Thursday, December 12, 2013 12:57 PM To: Cartun, Richard; Histonet Subject: [Histonet] RE: Freezer for tissue storage In my experience the uprights have more frost buildup problems, causing sealing problems. The chest ones I've had in the lab do less of this. Laurie -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cartun, Richard Sent: Thursday, December 12, 2013 12:52 PM To: Histonet Subject: [Histonet] Freezer for tissue storage I need a recommendation for a -80 degree C. freezer for storing tissue specimens. Do you prefer upright vs. chest? Thanks. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax richard.cartun@hhchealth.org This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ The contents of this message may contain private, protected and/or privileged information. If you received this message in error, you should destroy the e-mail message and any attachments or copies, and you are prohibited from retaining, distributing, disclosing or using any information contained within. Please contact the sender and advise of the erroneous delivery by return e-mail or telephone. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis <@t> bresnan.net Thu Dec 12 13:43:45 2013 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Thu Dec 12 13:44:08 2013 Subject: [Histonet] Re: Filtering stains Message-ID: <000201cef772$79e07a50$6da16ef0$@bresnan.net> Filtering stains was a long standing habit in our lab. It didn't matter if the solutions were coming from a stock bottle or reused and filtered into a clean container. However, the kind of filter paper is important and something I learned early on from Lillie and Fullmer's book, Histopathological Technic and Practical Histochemistry. It was recommended to NOT use a high retention paper (slow filtration) and use a medium fast paper. If the porous filter paper is too slow, dye molecules are retained on the paper plus it takes forever to filter a solution when tends to be thicker or flocculent. Nuclear Fast Red is one that takes forever to filter - very annoying when there was a time crunch. Plan ahead! If you use a fast paper e.g. more porous, then fine particles can go through the pores, creating some contamination on the section. Some stains were not filtered e.g. working silver solution for GMS. As for hematoxylin solutions, regardless of a new stock solution or reusing the stain the next day, we always filtered to get rid of any oxidation product and/or tissue particles. If I didn't filter hematoxylin solution before IHC counterstaining, there was always an ugly precipitate on the sections. Some stains in alcohol, e.g. Luxol fast blue and eosin were not filtered although reused solutions do contain tissue particles, and then were filtered. Rule in our lab: Filter stains through medium fast paper, daily for H&E, but top off to keep replenished and at correct level for staining racks. I know that some vendors say not to filter the hematoxylin before routine use, but I could never get out of the filtering habit. An addition bead of wisdom for people. Filtering is a messy procedure but using the correct funnel is important to prevent splashing. Years ago and in a chemistry class, I learned to use a tapered end funnel for filtering/introducing a fluid into a container. Make sure the tapered end touches the side of the container. Use wide, open ended (non-tapered) funnels are for introducing dry chemicals to a container as wide ends will cause splashing. Gayle Callis HTL/HT/MT(ASCP) From ttruscot <@t> vetmed.wsu.edu Thu Dec 12 18:14:19 2013 From: ttruscot <@t> vetmed.wsu.edu (Truscott, Tom) Date: Thu Dec 12 18:14:24 2013 Subject: [Histonet] Rodent eye paraffin microtomy In-Reply-To: References: <8D0C57107E22BB3-8AC-1C94@webmail-d156.sysops.aol.com> Message-ID: <9EF5279EBDFE6E4FB6605E8F183A00276A7F11C5@CVM76.vetmed.wsu.edu> Hi Jackie, I haven't done a lot of eyes, but mainly sheep lenses. I seemed to have more trouble with crushing artifact when trimming in- so I tried not to trim too close and do the closer trimming on the microtome- I also trimmed before they got too hard from fixing. Tom T -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bea DeBrosse-Serra Sent: Thursday, December 12, 2013 9:13 AM To: 'b427297@aol.com'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Rodent eye paraffin microtomy Let the eye soak in ice water before cutting for a very long time. Bea Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of b427297@aol.com Sent: Thursday, December 12, 2013 8:48 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Rodent eye paraffin microtomy I have a study coming up where obtaining perfect lens histology is critical. We do a pretty good job routinely, but since the lens is of interest, I would appreciate any tricks and techniques that can help us improve our paraffin lens histology within intact rodent eyes. Thanks, Jackie O' _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tony.henwood <@t> health.nsw.gov.au Thu Dec 12 18:58:59 2013 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Thu Dec 12 18:59:12 2013 Subject: [Histonet] RE: Freezer for tissue storage In-Reply-To: <9215BD4B0BA1B44D962A71C758B68D2E018A01C8@HHCEXCHMB05.hhcsystem.org> References: <9215BD4B0BA1B44D962A71C758B68D2E018A01C8@HHCEXCHMB05.hhcsystem.org> Message-ID: <6D6BD1DE8A5571489398B392A38A71579D479594@xmdb04.nch.kids> Hi Richard, I prefer Upright freezers and we have an excellent track record with the Sanyo VIP Freezer (one of our Sanyo low temp freezers is 15 years old and still working with no problems). http://us.sanyo.com/dynamic/product/Downloads/MDF-U33VC52VA53VC73VVIPSeriesBrochure-26928829.pdf Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cartun, Richard Sent: Friday, 13 December 2013 5:52 AM To: Histonet Subject: [Histonet] Freezer for tissue storage I need a recommendation for a -80 degree C. freezer for storing tissue specimens. Do you prefer upright vs. chest? Thanks. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax richard.cartun@hhchealth.org This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From epchatfield <@t> ihis.org Fri Dec 13 04:25:32 2013 From: epchatfield <@t> ihis.org (Elizabeth Chatfield) Date: Fri Dec 13 04:25:39 2013 Subject: [Histonet] B-RAF Message-ID: <52AAA85C020000BC00064B14@gwia-out.peigov> Happy Friday!! Has anyone out there worked up B-RAF on the Leica Bond? I've started playing with it, but I have a very short window before I leave my position and move on to another and would appreciate any input. Thanks, Elizabeth QEH Charlottetown, PE ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. From lcolbert <@t> pathmdlabs.com Fri Dec 13 11:01:05 2013 From: lcolbert <@t> pathmdlabs.com (Laurie Colbert) Date: Fri Dec 13 11:02:12 2013 Subject: [Histonet] Nails for fungal stain Message-ID: <12ECD7346266D74691EC2BFC75285E452F375261@BFL323E10.pathmdlabs.local> Our lab receives toenails, and we routinely do an H&E stain and a PAS stain for fungus. We receive the nails fresh, and when they are grossed they are placed directly into a purchased nail softening solution that is mainly potassium hydroxide before being placed on the tissue processor. My question: Would the potassium hydroxide have any bearing on the results of the fungal PAS since the nails are not fixed in formalin before going into the potassium hydroxide? Laurie Colbert, HT (ASCP) From TGoins <@t> mt.gov Fri Dec 13 11:38:15 2013 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Fri Dec 13 11:38:47 2013 Subject: [Histonet] RE: Nails for fungal stain In-Reply-To: <12ECD7346266D74691EC2BFC75285E452F375261@BFL323E10.pathmdlabs.local> References: <12ECD7346266D74691EC2BFC75285E452F375261@BFL323E10.pathmdlabs.local> Message-ID: Formalin does not affect carbohydrate structure so when the nail is fixed shouldn't matter. The KOH digests proteins so no deleterious effect on the fungal carbohydrate pre- or post-fixation. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Friday, December 13, 2013 10:01 AM To: Histonet Post (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Nails for fungal stain Our lab receives toenails, and we routinely do an H&E stain and a PAS stain for fungus. We receive the nails fresh, and when they are grossed they are placed directly into a purchased nail softening solution that is mainly potassium hydroxide before being placed on the tissue processor. My question: Would the potassium hydroxide have any bearing on the results of the fungal PAS since the nails are not fixed in formalin before going into the potassium hydroxide? Laurie Colbert, HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carol.wilson <@t> ricerca.com Fri Dec 13 12:54:40 2013 From: carol.wilson <@t> ricerca.com (Wilson, Carol) Date: Fri Dec 13 12:54:47 2013 Subject: [Histonet] Paraffin sections or formalin fixed tissue for EM - looking for reference lab Message-ID: <19848F1C0886A5409C729CC2136EDAFF4B2AFB9EFE@IAD2MBX09.mex02.mlsrvr.com> Hi All, Can anyone recommend a reference lab that can use paraffin sections or formalin fixed tissue for EM? Thanks in advance for suggestions. Carol Carol Wilson, HT(ASCP) Associate Scientist III Team Leader/Histopathology Ricerca Biosciences, LLC From hlukey <@t> msn.com Fri Dec 13 15:41:15 2013 From: hlukey <@t> msn.com (Hugh Luk) Date: Fri Dec 13 15:41:19 2013 Subject: [Histonet] Freezer for tissue storage In-Reply-To: References: Message-ID: Dr. Cartun, Agreed, chest freezers have less frost/door problems/sealing issues and seem to live longer. Stand-ups maximize footprint/space efficiency and allow for easier, horizontal-rack pulling for archiving and inventory. Due to volume and space constraints, the UH cancer center buys stand-ups. We have over a hundred. My personal caution is for the newer Thermo-Revco freezers, especially the ultra-low Thermo UXF in mostly 24.1 cu. ft.. Perhaps this is just our bad luck but an abnormal number failed (7/9), and three were within the first year. The unfortunate units were purchased over the last four years. Our bio-engineer has asked us to avoid buying Revco (after endorsing Revco/Harris for many years) and buy New Brunswick/Eppendorf or Sanyo ULT freezers, for the immediate future. We're not endorsing Sanyo or New Brunswick, we're just staying away from a brand that has recently given us trouble. Hopefully, it is different in your area, as Hawaii has logistical issues that may have exacerbated this issue (maybe it was that looong boat trip)? Just my $0.02. (And $0.02 will not buy anything, anymore.) Hugh UHCC path shared resource manager Honolulu, HI > ------------------------------ > > Date: Thu, 12 Dec 2013 18:51:57 +0000 > From: "Cartun, Richard" > Subject: [Histonet] Freezer for tissue storage > To: Histonet > Message-ID: > <9215BD4B0BA1B44D962A71C758B68D2E018A01C8@HHCEXCHMB05.hhcsystem.org> > Content-Type: text/plain; charset="iso-8859-1" > > I need a recommendation for a -80 degree C. freezer for storing tissue specimens. Do you prefer upright vs. chest? Thanks. > > > > Richard > > > > Richard W. Cartun, MS, PhD > > Director, Histology & Immunopathology > > Director, Biospecimen Collection Programs > > Assistant Director, Anatomic Pathology > > Hartford Hospital > > 80 Seymour Street > > Hartford, CT 06102 > > (860) 545-1596 Office > > (860) 545-2204 Fax > > richard.cartun@hhchealth.org > > This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. > ***************************************** From ttruscot <@t> vetmed.wsu.edu Fri Dec 13 15:57:20 2013 From: ttruscot <@t> vetmed.wsu.edu (Truscott, Tom) Date: Fri Dec 13 15:57:32 2013 Subject: [Histonet] Freezer for tissue storage In-Reply-To: References: Message-ID: <9EF5279EBDFE6E4FB6605E8F183A00276AF1503D@CVM77.vetmed.wsu.edu> We have both upright and chest and both have their pros and cons. We have had trouble with electrical circuits which aren't matched to the load that these freezers can put on them. An electrician told us that it can put an tremendous strain on the freezer motor if the circuit isn't right and two or three freezers start up at the same time- leading to shortened life of the freezer. So- make sure a good electrician is involved in your freezer set-up. Tom T -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hugh Luk Sent: Friday, December 13, 2013 1:41 PM To: histonet@lists.utsouthwestern.edu; Richard.Cartun@hhchealth.org Subject: [Histonet] Freezer for tissue storage Dr. Cartun, Agreed, chest freezers have less frost/door problems/sealing issues and seem to live longer. Stand-ups maximize footprint/space efficiency and allow for easier, horizontal-rack pulling for archiving and inventory. Due to volume and space constraints, the UH cancer center buys stand-ups. We have over a hundred. My personal caution is for the newer Thermo-Revco freezers, especially the ultra-low Thermo UXF in mostly 24.1 cu. ft.. Perhaps this is just our bad luck but an abnormal number failed (7/9), and three were within the first year. The unfortunate units were purchased over the last four years. Our bio-engineer has asked us to avoid buying Revco (after endorsing Revco/Harris for many years) and buy New Brunswick/Eppendorf or Sanyo ULT freezers, for the immediate future. We're not endorsing Sanyo or New Brunswick, we're just staying away from a brand that has recently given us trouble. Hopefully, it is different in your area, as Hawaii has logistical issues that may have exacerbated this issue (maybe it was that looong boat trip)? Just my $0.02. (And $0.02 will not buy anything, anymore.) Hugh UHCC path shared resource manager Honolulu, HI > ------------------------------ > > Date: Thu, 12 Dec 2013 18:51:57 +0000 > From: "Cartun, Richard" > Subject: [Histonet] Freezer for tissue storage > To: Histonet > Message-ID: > <9215BD4B0BA1B44D962A71C758B68D2E018A01C8@HHCEXCHMB05.hhcsystem.org> > Content-Type: text/plain; charset="iso-8859-1" > > I need a recommendation for a -80 degree C. freezer for storing tissue specimens. Do you prefer upright vs. chest? Thanks. > > > > Richard > > > > Richard W. Cartun, MS, PhD > > Director, Histology & Immunopathology > > Director, Biospecimen Collection Programs > > Assistant Director, Anatomic Pathology > > Hartford Hospital > > 80 Seymour Street > > Hartford, CT 06102 > > (860) 545-1596 Office > > (860) 545-2204 Fax > > richard.cartun@hhchealth.org > > This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. > ***************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From levi.fried <@t> gmail.com Sat Dec 14 10:25:08 2013 From: levi.fried <@t> gmail.com (Levi Fried) Date: Sat Dec 14 10:25:37 2013 Subject: [Histonet] Staining on Alcohol Fixed Smears. Message-ID: Hi Everyone. I am looking to stain alcohol fixed smears with a group of antibodies. The antibodies of particular interest are p63, p53 and ki67. Along with these antibodies I am looking for positive nuclear and cytoplastic staining antibodies. If anyone has any experience in staining alcohol fixed slides your information is very appreciated. All the best. -- Sincerely, - Levi Fried From bethcoxx <@t> gmail.com Sat Dec 14 17:30:20 2013 From: bethcoxx <@t> gmail.com (Beth Cox) Date: Sat Dec 14 17:30:26 2013 Subject: [Histonet] Re: Staining on Alcohol Fixed Smears In-Reply-To: <52ac9cc3.a9dbb60a.3c47.749cSMTPIN_ADDED_MISSING@mx.google.com> References: <52ac9cc3.a9dbb60a.3c47.749cSMTPIN_ADDED_MISSING@mx.google.com> Message-ID: <52ACEA0C.7050600@gmail.com> Levi, I've worked up most of the more common antibodies on alcohol fixed smears. There are two things to remember here: 1) your specimens are alcohol fixed, meaning antigen retrieval needs are different, and 2) they are smears, meaning the cells weren't 'cut open' during microtomy, which impeeds some nuclear staining. Those are two different issues to consider. Some rules of thumb: 1) most cell membrane antigens will stain nicely with minimal changes to the protocols you use for FFPE specimens 2) most cytokeratin antigens stain nicely with little change to your FFPE protocols (if you need to change, start by cutting your antigen retrieval time in half) 3) most nuclear antigens require significant changes from FFPE protocols, and some are essentially impossible to stain using standard antibodies. When I return to work on Monday, I will forward you my protocol 'adjustments' for the antibodies you requested. Beth Cox, HTL/SCT(ASCP)QIHC Vagabond Histotech On 12/14/2013 12:00 PM, histonet-request@lists.utsouthwestern.edu wrote: > Message: 4 > Date: Sat, 14 Dec 2013 18:25:08 +0200 > From: Levi Fried > Subject: [Histonet] Staining on Alcohol Fixed Smears. > To:histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset=ISO-8859-1 > > Hi Everyone. > > I am looking to stain alcohol fixed smears with a group of antibodies. > > The antibodies of particular interest are p63, p53 and ki67. > Along with these antibodies I am looking for positive nuclear and > cytoplastic staining antibodies. > > If anyone has any experience in staining alcohol fixed slides your > information is very appreciated. > > All the best. > > -- Sincerely, - Levi Fried From dhenders01 <@t> msn.com Sun Dec 15 09:48:53 2013 From: dhenders01 <@t> msn.com (DAVID HENDERSON) Date: Sun Dec 15 09:48:58 2013 Subject: [Histonet] RE: Wax on floor: An Easy Solution. Message-ID: I use WD 40 and 4x4" gauze pads. 1. Squirt WD 40 using a focused spout straw directly on a small (1-2 square ft.) area of waxy floor. Apply just enough to wet only that area. 2. Wait a few seconds for the WD 40 to loosen the wax. 3. Place a short stack of (4-6) gauze pads on the near edge of the soaked area. 4. Maintain secure balance with one foot on an adjacent non-oily area while placing the ball of the other foot on the gauze stack. 5. Gradually transfer enough weight to the gauze stack to provide sufficient force to scrub off all the wax beneath the pad using alternating twisting and sliding action of the scrubbing foot. Let your weight do most of the work. 6. Depending on the amount of wax and embedded grime, you may need to periodically remove and dispose of the bottom 1-2 pads of the stack as they become clogged with dirty wax (gloves recommended), and add 1-2 clean pads to the top of the stack. 7. Finish off the cleaned area by removing all remaining WD 40 with clean gauze pad short stacks using light foot wiping action. 8. Repeat above steps on adjacent areas. Cheers! David Henderson, Ph.D., HTL RML, Tulsa, OK Sent from my iPad From fbozkurt <@t> gmail.com Mon Dec 16 01:02:54 2013 From: fbozkurt <@t> gmail.com (Mehmet Fatih BOZKURT) Date: Mon Dec 16 01:02:58 2013 Subject: [Histonet] rabbit cea antibody for mouse Message-ID: Hello, I'm looking for rabbit CEA antibody for using on mouse tissue. Anyone can help me? Thanks. -- Mehmet Fatih BOZKURT, DVM, PhD Afyon Kocatepe University Faculty of Veterinary Medicine Department of Pathology 03030, ANS Campus Afyonkarahisar-TURKEY Tel: +902722281312-16173/16237 From Pat.Patterson <@t> propath.com Mon Dec 16 08:41:51 2013 From: Pat.Patterson <@t> propath.com (Pat Patterson) Date: Mon Dec 16 08:41:56 2013 Subject: [Histonet] IHC Lead Tech Position - Dallas Message-ID: <6DCB8B92D0138244B56CE8EACE0D458D1675B38A@Mail.propathlab.com> LEAD IMMUNOHISTOCHEMISTRY TECHNICIAN ProPath, a progressive, CAP accredited, high-volume pathology practice in Dallas, Texas is seeking an Lead Immunohistochemistry Technician for its' Immunohistochemistry Lab. Responsibilities include slide preparation (paraffin and frozen sections), IHC staining using our unique manual system, antibody titer preparation, equipment maintenance, supply/reagent inventory maintenance, and QC/QA recording. The ideal candidate will have a minimum of 5 years Histology experience with paraffin microtomy with a variety of different tissue types, prefer at least 3 years immunohistochemistry, immunofluorescence or in situ hybridization and frozen section experience. Technical Lead/Coordinator experience is required. Working knowledge of IHC theory required, hands on IHC performance desired. If using an automated system we'll easily train you on our manual system. HT (ASCP) or QIHC desired. The hours for the position are 7:00 p.m. to 3:30 a.m. Monday through Friday. ProPath utilizes leading technology and is a quality oriented pathology laboratory. Benefits include medical, dental, Short and Long Term Disability insurance, a matched 401K plan and more! Don't Follow the Leader! Join the Leader! To apply, please visit www.propath.com EOE Accessibility Accommodations If you require an accommodation to navigate or apply to our careers site, please send your request to accessibility@propath.com. Pat Patterson, HTL(ASCP) Supervisor, Immunohistochemistry ProPath - The Leader in Pathology Services 1355 River Bend Drive Dallas, TX 75247 pat.patterson@propath.com 214-237-1700 x 2027 214-237-1730 fax To learn more about ProPath, please visit http://www.ProPath.com This electronic message is intended to be for the use only of the named recipient and may contain information that is confidential or privileged. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this message in error or are not the named recipient, please notify us immediately by contacting the sender at the electronic mail address noted above, and delete and destroy all copies of this message. From WesterM <@t> MedImmune.com Mon Dec 16 08:56:10 2013 From: WesterM <@t> MedImmune.com (Wester, Martha) Date: Mon Dec 16 08:56:21 2013 Subject: [Histonet] Freezer for tissue storage Message-ID: <2FF3ED05D0191D42A22022D82494056D6DD22A42@MBPRD0002.medimmune.com> Dr Cartun- We've had similar experiences to Hugh's. In the histo group we maybe only have 15 validated freezers (all Revco's), but throughout our company we have hundreds. Over the past few years our Research Operations has moved to purchasing Sanyos because of the turnover rates of the new ultra low Revco's. We have no Sanyo's in our group yet, so I do not have any feedback on their performance. (I've always used uprights, so I'm not much use there either.) Best of luck! Martha Lab Manager Gaithersburg, MD ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Message: 2 Date: Fri, 13 Dec 2013 11:41:15 -1000 From: Hugh Luk Subject: [Histonet] Freezer for tissue storage To: "histonet@lists.utsouthwestern.edu" , "Richard.Cartun@hhchealth.org" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Dr. Cartun, Agreed, chest freezers have less frost/door problems/sealing issues and seem to live longer. Stand-ups maximize footprint/space efficiency and allow for easier, horizontal-rack pulling for archiving and inventory. Due to volume and space constraints, the UH cancer center buys stand-ups. We have over a hundred. My personal caution is for the newer Thermo-Revco freezers, especially the ultra-low Thermo UXF in mostly 24.1 cu. ft.. Perhaps this is just our bad luck but an abnormal number failed (7/9), and three were within the first year. The unfortunate units were purchased over the last four years. Our bio-engineer has asked us to avoid buying Revco (after endorsing Revco/Harris for many years) and buy New Brunswick/Eppendorf or Sanyo ULT freezers, for the immediate future. We're not endorsing Sanyo or New Brunswick, we're just staying away from a brand that has recently given us trouble. Hopefully, it is different in your area, as Hawaii has logistical issues that may have exacerbated this issue (maybe it was that looong boat trip)? Just my $0.02. (And $0.02 will not buy anything, anymore.) Hugh UHCC path shared resource manager Honolulu, HI > ------------------------------ > > Date: Thu, 12 Dec 2013 18:51:57 +0000 > From: "Cartun, Richard" > Subject: [Histonet] Freezer for tissue storage > To: Histonet > Message-ID: > <9215BD4B0BA1B44D962A71C758B68D2E018A01C8@HHCEXCHMB05.hhcsystem.org> > Content-Type: text/plain; charset="iso-8859-1" > > I need a recommendation for a -80 degree C. freezer for storing tissue specimens. Do you prefer upright vs. chest? Thanks. > > > > Richard > > > > Richard W. Cartun, MS, PhD > > Director, Histology & Immunopathology > > Director, Biospecimen Collection Programs > > Assistant Director, Anatomic Pathology > > Hartford Hospital > > 80 Seymour Street > > Hartford, CT 06102 > > (860) 545-1596 Office > > (860) 545-2204 Fax > > richard.cartun@hhchealth.org > > This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. > ***************************************** ------------------------------ Message: 3 Date: Fri, 13 Dec 2013 21:57:20 +0000 From: "Truscott, Tom" Subject: RE: [Histonet] Freezer for tissue storage To: Hugh Luk , "histonet@lists.utsouthwestern.edu" , "Richard.Cartun@hhchealth.org" Message-ID: <9EF5279EBDFE6E4FB6605E8F183A00276AF1503D@CVM77.vetmed.wsu.edu> Content-Type: text/plain; charset="us-ascii" We have both upright and chest and both have their pros and cons. We have had trouble with electrical circuits which aren't matched to the load that these freezers can put on them. An electrician told us that it can put an tremendous strain on the freezer motor if the circuit isn't right and two or three freezers start up at the same time- leading to shortened life of the freezer. So- make sure a good electrician is involved in your freezer set-up. Tom T -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hugh Luk Sent: Friday, December 13, 2013 1:41 PM To: histonet@lists.utsouthwestern.edu; Richard.Cartun@hhchealth.org Subject: [Histonet] Freezer for tissue storage Dr. Cartun, Agreed, chest freezers have less frost/door problems/sealing issues and seem to live longer. Stand-ups maximize footprint/space efficiency and allow for easier, horizontal-rack pulling for archiving and inventory. Due to volume and space constraints, the UH cancer center buys stand-ups. We have over a hundred. My personal caution is for the newer Thermo-Revco freezers, especially the ultra-low Thermo UXF in mostly 24.1 cu. ft.. Perhaps this is just our bad luck but an abnormal number failed (7/9), and three were within the first year. The unfortunate units were purchased over the last four years. Our bio-engineer has asked us to avoid buying Revco (after endorsing Revco/Harris for many years) and buy New Brunswick/Eppendorf or Sanyo ULT freezers, for the immediate future. We're not endorsing Sanyo or New Brunswick, we're just staying away from a brand that has recently given us trouble. Hopefully, it is different in your area, as Hawaii has logistical issues that may have exacerbated this issue (maybe it was that looong boat trip)? Just my $0.02. (And $0.02 will not buy anything, anymore.) Hugh UHCC path shared resource manager Honolulu, HI > ------------------------------ > > Date: Thu, 12 Dec 2013 18:51:57 +0000 > From: "Cartun, Richard" > Subject: [Histonet] Freezer for tissue storage > To: Histonet > Message-ID: > <9215BD4B0BA1B44D962A71C758B68D2E018A01C8@HHCEXCHMB05.hhcsystem.org> > Content-Type: text/plain; charset="iso-8859-1" > > I need a recommendation for a -80 degree C. freezer for storing tissue specimens. Do you prefer upright vs. chest? Thanks. > > > > Richard > > > > Richard W. Cartun, MS, PhD > > Director, Histology & Immunopathology > > Director, Biospecimen Collection Programs > > Assistant Director, Anatomic Pathology > > Hartford Hospital > > 80 Seymour Street > > Hartford, CT 06102 > > (860) 545-1596 Office > > (860) 545-2204 Fax > > richard.cartun@hhchealth.org > > This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. > ***************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Sat, 14 Dec 2013 18:25:08 +0200 From: Levi Fried Subject: [Histonet] Staining on Alcohol Fixed Smears. To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hi Everyone. I am looking to stain alcohol fixed smears with a group of antibodies. The antibodies of particular interest are p63, p53 and ki67. Along with these antibodies I am looking for positive nuclear and cytoplastic staining antibodies. If anyone has any experience in staining alcohol fixed slides your information is very appreciated. All the best. -- Sincerely, - Levi Fried ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 121, Issue 19 ***************************************** To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. From TMcNemar <@t> lmhealth.org Mon Dec 16 10:13:59 2013 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Mon Dec 16 10:14:08 2013 Subject: [Histonet] Re: Staining on Alcohol Fixed Smears In-Reply-To: <52ACEA0C.7050600@gmail.com> References: <52ac9cc3.a9dbb60a.3c47.749cSMTPIN_ADDED_MISSING@mx.google.com> <52ACEA0C.7050600@gmail.com> Message-ID: Since there is no exposure to formalin, we do not do antigen retrieval on alcohol fixed specimens. We use the Ventana Benchmark XT and copy the protocol to a new number and use the "Wet" option rather than paraffin. No retrieval needed. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Beth Cox Sent: Saturday, December 14, 2013 6:30 PM To: histonet@lists.utsouthwestern.edu; levi.fried@gmail.com Subject: [Histonet] Re: Staining on Alcohol Fixed Smears Levi, I've worked up most of the more common antibodies on alcohol fixed smears. There are two things to remember here: 1) your specimens are alcohol fixed, meaning antigen retrieval needs are different, and 2) they are smears, meaning the cells weren't 'cut open' during microtomy, which impeeds some nuclear staining. Those are two different issues to consider. Some rules of thumb: 1) most cell membrane antigens will stain nicely with minimal changes to the protocols you use for FFPE specimens 2) most cytokeratin antigens stain nicely with little change to your FFPE protocols (if you need to change, start by cutting your antigen retrieval time in half) 3) most nuclear antigens require significant changes from FFPE protocols, and some are essentially impossible to stain using standard antibodies. When I return to work on Monday, I will forward you my protocol 'adjustments' for the antibodies you requested. Beth Cox, HTL/SCT(ASCP)QIHC Vagabond Histotech On 12/14/2013 12:00 PM, histonet-request@lists.utsouthwestern.edu wrote: > Message: 4 > Date: Sat, 14 Dec 2013 18:25:08 +0200 > From: Levi Fried > Subject: [Histonet] Staining on Alcohol Fixed Smears. > To:histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset=ISO-8859-1 > > Hi Everyone. > > I am looking to stain alcohol fixed smears with a group of antibodies. > > The antibodies of particular interest are p63, p53 and ki67. > Along with these antibodies I am looking for positive nuclear and > cytoplastic staining antibodies. > > If anyone has any experience in staining alcohol fixed slides your > information is very appreciated. > > All the best. > > -- Sincerely, - Levi Fried _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From Vickroy.Jim <@t> mhsil.com Mon Dec 16 10:16:46 2013 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Mon Dec 16 10:16:51 2013 Subject: [Histonet] Charges for recuts on legal cases Message-ID: Can anyone share with me what they charge for doing recuts on cases that have been pulled for legal purposes? I am trying to see what others charge before we assign a charge. I think most people are probably charging by the slide but please let me know if you charge differently. Thanks James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From Wanda.Smith <@t> HCAhealthcare.com Mon Dec 16 12:54:21 2013 From: Wanda.Smith <@t> HCAhealthcare.com (Wanda.Smith@HCAhealthcare.com) Date: Mon Dec 16 12:54:40 2013 Subject: [Histonet] RE: Charges for recuts on legal cases In-Reply-To: References: Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BA27E7865B35@NADCWPMSGCMS03.hca.corpad.net> I charge a $30.00 fee for investigating the case and issuing a report. I offer 1 H&E and 2 unstained sections on plus slides and charge an additional $15.00 per slide. They furnish the shipping/FedEx. W WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC? 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Monday, December 16, 2013 11:17 AM To: histonet@lists.utsouthwestern.edu Cc: Bantner, Diane Subject: [Histonet] Charges for recuts on legal cases Can anyone share with me what they charge for doing recuts on cases that have been pulled for legal purposes? I am trying to see what others charge before we assign a charge. I think most people are probably charging by the slide but please let me know if you charge differently. Thanks James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bethcoxx <@t> gmail.com Mon Dec 16 13:24:51 2013 From: bethcoxx <@t> gmail.com (Beth Cox) Date: Mon Dec 16 13:24:56 2013 Subject: [Histonet] Re: Staining on Alcohol Fixed Smears Message-ID: <52AF5383.4080103@gmail.com> I agree that with no formalin exposure, there is no crosslinking that needs to be broken, but a "then, do nothing" approach to antigen retrieval oversimplifies the issue. Alcohol fixation makes changes in the protiens/antibodies also, although different changes than formalin does, and these changes need to be accounted for. In my research on alcohol fixed smears, I found that most antibodies perform best with some antigen retrieval, often gentler than formalin tissues require. Beth Cox, HTL/SCT(ASCP)QIHC Vagabond Histotech ------------------------------ Message: 4 Date: Mon, 16 Dec 2013 11:13:59 -0500 From: Tom McNemar Subject: RE: [Histonet] Re: Staining on Alcohol Fixed Smears To: 'Beth Cox', "histonet@lists.utsouthwestern.edu" ,"levi.fried@gmail.com" Message-ID: Content-Type: text/plain; charset="us-ascii" Since there is no exposure to formalin, we do not do antigen retrieval on alcohol fixed specimens. We use the Ventana Benchmark XT and copy the protocol to a new number and use the "Wet" option rather than paraffin. No retrieval needed. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From:histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Beth Cox Sent: Saturday, December 14, 2013 6:30 PM To:histonet@lists.utsouthwestern.edu;levi.fried@gmail.com Subject: [Histonet] Re: Staining on Alcohol Fixed Smears Levi, I've worked up most of the more common antibodies on alcohol fixed smears. There are two things to remember here: 1) your specimens are alcohol fixed, meaning antigen retrieval needs are different, and 2) they are smears, meaning the cells weren't 'cut open' during microtomy, which impeeds some nuclear staining. Those are two different issues to consider. Some rules of thumb: 1) most cell membrane antigens will stain nicely with minimal changes to the protocols you use for FFPE specimens 2) most cytokeratin antigens stain nicely with little change to your FFPE protocols (if you need to change, start by cutting your antigen retrieval time in half) 3) most nuclear antigens require significant changes from FFPE protocols, and some are essentially impossible to stain using standard antibodies. When I return to work on Monday, I will forward you my protocol 'adjustments' for the antibodies you requested. Beth Cox, HTL/SCT(ASCP)QIHC Vagabond Histotech On 12/14/2013 12:00 PM,histonet-request@lists.utsouthwestern.edu wrote: > Message: 4 > Date: Sat, 14 Dec 2013 18:25:08 +0200 > From: Levi Fried > Subject: [Histonet] Staining on Alcohol Fixed Smears. > To:histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset=ISO-8859-1 > > Hi Everyone. > > I am looking to stain alcohol fixed smears with a group of antibodies. > > The antibodies of particular interest are p63, p53 and ki67. > Along with these antibodies I am looking for positive nuclear and > cytoplastic staining antibodies. > > If anyone has any experience in staining alcohol fixed slides your > information is very appreciated. > > All the best. > > -- Sincerely, - Levi Fried From sae2001 <@t> med.cornell.edu Mon Dec 16 15:13:46 2013 From: sae2001 <@t> med.cornell.edu (Scott A. Ely) Date: Mon Dec 16 15:14:30 2013 Subject: [Histonet] RE: Histonet Digest, Vol 121, Issue 21 In-Reply-To: <9b6584c5-2ccc-4e4e-98f0-8b1b47fe04bd@NYSGCAS03.a.wcmc-ad.net> References: <9b6584c5-2ccc-4e4e-98f0-8b1b47fe04bd@NYSGCAS03.a.wcmc-ad.net> Message-ID: re: per slide cost for IHC I realize there are differences in reagents, including antibody prices. Still, I sometimes am asked to provide the average cost to produce a single IHC slide. If anyone has an available estimate, especially broken down (glass cost, antibody cost, labor cost...), I would greatly appreciate it. Thank you. ------------------------------------------------------------------------------- Scott Ely, MD MPH Associate Director, Hematopathology Fellowship Program Section of Hematopathology Department of Pathology Weill Medical College of Cornell University New York Presbyterian Hospital Room: Starr 715 525 E. 68th Street New York, NY 10065 PH: 212-746-2442 FAX: 212-746-2009 http://www.cornellphysicians.com/scottely/ Legal Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the original intended recipient(s) selected by Dr. Ely and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the recipient specified by Dr. Ely, please contact the sender by reply e-mail and destroy all copies of the original message. ------------------------------------------------------------------------------- ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of histonet-request@lists.utsouthwestern.edu [histonet-request@lists.utsouthwestern.edu] Sent: Monday, December 16, 2013 1:07 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 121, Issue 21 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. rabbit cea antibody for mouse (Mehmet Fatih BOZKURT) 2. IHC Lead Tech Position - Dallas (Pat Patterson) 3. Freezer for tissue storage (Wester, Martha) 4. RE: Re: Staining on Alcohol Fixed Smears (Tom McNemar) 5. Charges for recuts on legal cases (Vickroy, Jim) ---------------------------------------------------------------------- Message: 1 Date: Mon, 16 Dec 2013 09:02:54 +0200 From: Mehmet Fatih BOZKURT Subject: [Histonet] rabbit cea antibody for mouse To: ihcrg@googlegroups.com, Histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hello, I'm looking for rabbit CEA antibody for using on mouse tissue. Anyone can help me? Thanks. -- Mehmet Fatih BOZKURT, DVM, PhD Afyon Kocatepe University Faculty of Veterinary Medicine Department of Pathology 03030, ANS Campus Afyonkarahisar-TURKEY Tel: +902722281312-16173/16237 ------------------------------ Message: 2 Date: Mon, 16 Dec 2013 14:41:51 +0000 From: Pat Patterson Subject: [Histonet] IHC Lead Tech Position - Dallas To: "histonet@lists.utsouthwestern.edu" Message-ID: <6DCB8B92D0138244B56CE8EACE0D458D1675B38A@Mail.propathlab.com> Content-Type: text/plain; charset="us-ascii" LEAD IMMUNOHISTOCHEMISTRY TECHNICIAN ProPath, a progressive, CAP accredited, high-volume pathology practice in Dallas, Texas is seeking an Lead Immunohistochemistry Technician for its' Immunohistochemistry Lab. Responsibilities include slide preparation (paraffin and frozen sections), IHC staining using our unique manual system, antibody titer preparation, equipment maintenance, supply/reagent inventory maintenance, and QC/QA recording. The ideal candidate will have a minimum of 5 years Histology experience with paraffin microtomy with a variety of different tissue types, prefer at least 3 years immunohistochemistry, immunofluorescence or in situ hybridization and frozen section experience. Technical Lead/Coordinator experience is required. Working knowledge of IHC theory required, hands on IHC performance desired. If using an automated system we'll easily train you on our manual system. HT (ASCP) or QIHC desired. The hours for the position are 7:00 p.m. to 3:30 a.m. Monday through Friday. ProPath utilizes leading technology and is a quality oriented pathology laboratory. Benefits include medical, dental, Short and Long Term Disability insurance, a matched 401K plan and more! Don't Follow the Leader! Join the Leader! To apply, please visit www.propath.com EOE Accessibility Accommodations If you require an accommodation to navigate or apply to our careers site, please send your request to accessibility@propath.com. Pat Patterson, HTL(ASCP) Supervisor, Immunohistochemistry ProPath - The Leader in Pathology Services 1355 River Bend Drive Dallas, TX 75247 pat.patterson@propath.com 214-237-1700 x 2027 214-237-1730 fax To learn more about ProPath, please visit http://www.ProPath.com This electronic message is intended to be for the use only of the named recipient and may contain information that is confidential or privileged. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this message in error or are not the named recipient, please notify us immediately by contacting the sender at the electronic mail address noted above, and delete and destroy all copies of this message. ------------------------------ Message: 3 Date: Mon, 16 Dec 2013 14:56:10 +0000 From: "Wester, Martha" Subject: [Histonet] Freezer for tissue storage To: "histonet@lists.utsouthwestern.edu" Message-ID: <2FF3ED05D0191D42A22022D82494056D6DD22A42@MBPRD0002.medimmune.com> Content-Type: text/plain; charset="us-ascii" Dr Cartun- We've had similar experiences to Hugh's. In the histo group we maybe only have 15 validated freezers (all Revco's), but throughout our company we have hundreds. Over the past few years our Research Operations has moved to purchasing Sanyos because of the turnover rates of the new ultra low Revco's. We have no Sanyo's in our group yet, so I do not have any feedback on their performance. (I've always used uprights, so I'm not much use there either.) Best of luck! Martha Lab Manager Gaithersburg, MD ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Message: 2 Date: Fri, 13 Dec 2013 11:41:15 -1000 From: Hugh Luk Subject: [Histonet] Freezer for tissue storage To: "histonet@lists.utsouthwestern.edu" , "Richard.Cartun@hhchealth.org" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Dr. Cartun, Agreed, chest freezers have less frost/door problems/sealing issues and seem to live longer. Stand-ups maximize footprint/space efficiency and allow for easier, horizontal-rack pulling for archiving and inventory. Due to volume and space constraints, the UH cancer center buys stand-ups. We have over a hundred. My personal caution is for the newer Thermo-Revco freezers, especially the ultra-low Thermo UXF in mostly 24.1 cu. ft.. Perhaps this is just our bad luck but an abnormal number failed (7/9), and three were within the first year. The unfortunate units were purchased over the last four years. Our bio-engineer has asked us to avoid buying Revco (after endorsing Revco/Harris for many years) and buy New Brunswick/Eppendorf or Sanyo ULT freezers, for the immediate future. We're not endorsing Sanyo or New Brunswick, we're just staying away from a brand that has recently given us trouble. Hopefully, it is different in your area, as Hawaii has logistical issues that may have exacerbated this issue (maybe it was that looong boat trip)? Just my $0.02. (And $0.02 will not buy anything, anymore.) Hugh UHCC path shared resource manager Honolulu, HI > ------------------------------ > > Date: Thu, 12 Dec 2013 18:51:57 +0000 > From: "Cartun, Richard" > Subject: [Histonet] Freezer for tissue storage > To: Histonet > Message-ID: > <9215BD4B0BA1B44D962A71C758B68D2E018A01C8@HHCEXCHMB05.hhcsystem.org> > Content-Type: text/plain; charset="iso-8859-1" > > I need a recommendation for a -80 degree C. freezer for storing tissue specimens. Do you prefer upright vs. chest? Thanks. > > > > Richard > > > > Richard W. Cartun, MS, PhD > > Director, Histology & Immunopathology > > Director, Biospecimen Collection Programs > > Assistant Director, Anatomic Pathology > > Hartford Hospital > > 80 Seymour Street > > Hartford, CT 06102 > > (860) 545-1596 Office > > (860) 545-2204 Fax > > richard.cartun@hhchealth.org > > This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. > ***************************************** ------------------------------ Message: 3 Date: Fri, 13 Dec 2013 21:57:20 +0000 From: "Truscott, Tom" Subject: RE: [Histonet] Freezer for tissue storage To: Hugh Luk , "histonet@lists.utsouthwestern.edu" , "Richard.Cartun@hhchealth.org" Message-ID: <9EF5279EBDFE6E4FB6605E8F183A00276AF1503D@CVM77.vetmed.wsu.edu> Content-Type: text/plain; charset="us-ascii" We have both upright and chest and both have their pros and cons. We have had trouble with electrical circuits which aren't matched to the load that these freezers can put on them. An electrician told us that it can put an tremendous strain on the freezer motor if the circuit isn't right and two or three freezers start up at the same time- leading to shortened life of the freezer. So- make sure a good electrician is involved in your freezer set-up. Tom T -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hugh Luk Sent: Friday, December 13, 2013 1:41 PM To: histonet@lists.utsouthwestern.edu; Richard.Cartun@hhchealth.org Subject: [Histonet] Freezer for tissue storage Dr. Cartun, Agreed, chest freezers have less frost/door problems/sealing issues and seem to live longer. Stand-ups maximize footprint/space efficiency and allow for easier, horizontal-rack pulling for archiving and inventory. Due to volume and space constraints, the UH cancer center buys stand-ups. We have over a hundred. My personal caution is for the newer Thermo-Revco freezers, especially the ultra-low Thermo UXF in mostly 24.1 cu. ft.. Perhaps this is just our bad luck but an abnormal number failed (7/9), and three were within the first year. The unfortunate units were purchased over the last four years. Our bio-engineer has asked us to avoid buying Revco (after endorsing Revco/Harris for many years) and buy New Brunswick/Eppendorf or Sanyo ULT freezers, for the immediate future. We're not endorsing Sanyo or New Brunswick, we're just staying away from a brand that has recently given us trouble. Hopefully, it is different in your area, as Hawaii has logistical issues that may have exacerbated this issue (maybe it was that looong boat trip)? Just my $0.02. (And $0.02 will not buy anything, anymore.) Hugh UHCC path shared resource manager Honolulu, HI > ------------------------------ > > Date: Thu, 12 Dec 2013 18:51:57 +0000 > From: "Cartun, Richard" > Subject: [Histonet] Freezer for tissue storage > To: Histonet > Message-ID: > <9215BD4B0BA1B44D962A71C758B68D2E018A01C8@HHCEXCHMB05.hhcsystem.org> > Content-Type: text/plain; charset="iso-8859-1" > > I need a recommendation for a -80 degree C. freezer for storing tissue specimens. Do you prefer upright vs. chest? Thanks. > > > > Richard > > > > Richard W. Cartun, MS, PhD > > Director, Histology & Immunopathology > > Director, Biospecimen Collection Programs > > Assistant Director, Anatomic Pathology > > Hartford Hospital > > 80 Seymour Street > > Hartford, CT 06102 > > (860) 545-1596 Office > > (860) 545-2204 Fax > > richard.cartun@hhchealth.org > > This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. > ***************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Sat, 14 Dec 2013 18:25:08 +0200 From: Levi Fried Subject: [Histonet] Staining on Alcohol Fixed Smears. To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hi Everyone. I am looking to stain alcohol fixed smears with a group of antibodies. The antibodies of particular interest are p63, p53 and ki67. Along with these antibodies I am looking for positive nuclear and cytoplastic staining antibodies. If anyone has any experience in staining alcohol fixed slides your information is very appreciated. All the best. -- Sincerely, - Levi Fried ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 121, Issue 19 ***************************************** To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. ------------------------------ Message: 4 Date: Mon, 16 Dec 2013 11:13:59 -0500 From: Tom McNemar Subject: RE: [Histonet] Re: Staining on Alcohol Fixed Smears To: 'Beth Cox' , "histonet@lists.utsouthwestern.edu" , "levi.fried@gmail.com" Message-ID: Content-Type: text/plain; charset="us-ascii" Since there is no exposure to formalin, we do not do antigen retrieval on alcohol fixed specimens. We use the Ventana Benchmark XT and copy the protocol to a new number and use the "Wet" option rather than paraffin. No retrieval needed. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Beth Cox Sent: Saturday, December 14, 2013 6:30 PM To: histonet@lists.utsouthwestern.edu; levi.fried@gmail.com Subject: [Histonet] Re: Staining on Alcohol Fixed Smears Levi, I've worked up most of the more common antibodies on alcohol fixed smears. There are two things to remember here: 1) your specimens are alcohol fixed, meaning antigen retrieval needs are different, and 2) they are smears, meaning the cells weren't 'cut open' during microtomy, which impeeds some nuclear staining. Those are two different issues to consider. Some rules of thumb: 1) most cell membrane antigens will stain nicely with minimal changes to the protocols you use for FFPE specimens 2) most cytokeratin antigens stain nicely with little change to your FFPE protocols (if you need to change, start by cutting your antigen retrieval time in half) 3) most nuclear antigens require significant changes from FFPE protocols, and some are essentially impossible to stain using standard antibodies. When I return to work on Monday, I will forward you my protocol 'adjustments' for the antibodies you requested. Beth Cox, HTL/SCT(ASCP)QIHC Vagabond Histotech On 12/14/2013 12:00 PM, histonet-request@lists.utsouthwestern.edu wrote: > Message: 4 > Date: Sat, 14 Dec 2013 18:25:08 +0200 > From: Levi Fried > Subject: [Histonet] Staining on Alcohol Fixed Smears. > To:histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset=ISO-8859-1 > > Hi Everyone. > > I am looking to stain alcohol fixed smears with a group of antibodies. > > The antibodies of particular interest are p63, p53 and ki67. > Along with these antibodies I am looking for positive nuclear and > cytoplastic staining antibodies. > > If anyone has any experience in staining alcohol fixed slides your > information is very appreciated. > > All the best. > > -- Sincerely, - Levi Fried _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ------------------------------ Message: 5 Date: Mon, 16 Dec 2013 10:16:46 -0600 From: "Vickroy, Jim" Subject: [Histonet] Charges for recuts on legal cases To: "histonet@lists.utsouthwestern.edu" Cc: "Bantner, Diane" Message-ID: Content-Type: text/plain; charset="us-ascii" Can anyone share with me what they charge for doing recuts on cases that have been pulled for legal purposes? I am trying to see what others charge before we assign a charge. I think most people are probably charging by the slide but please let me know if you charge differently. Thanks James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 121, Issue 21 ***************************************** From Reuel.Cornelia <@t> tsrh.org Mon Dec 16 15:40:40 2013 From: Reuel.Cornelia <@t> tsrh.org (Reuel Cornelia) Date: Mon Dec 16 15:40:47 2013 Subject: [Histonet] Perfusion of mouse bone femur Message-ID: <52AF1EF8.077E.00C5.1@tsrh.org> Does anybody have a Protocol for perfusion of mouse bone femur. It looks like my protocol does not work. 1.fix in 4% PFA in PBS for 24 hrs in refrigerator 2. wash in PBS 3x 3. transfer in 14 %EDTA for 5 days. 4. Wash in water for maximum of 2 hrs in running water 6. perfusion with 15% sucrose overnight 7.perfusion with 30% overnight. 8. Transfer in OCT. 8. Cut in 10 um and transfer section on APES slide Results: When cutting section, the tissue does not adhere to the slides properly. Can you please help us or any comment to correct this protocol. Reuel Cornelia, BS MT, AMT Cellular Pathology Texas Scottish Rite Hospital for Children 2222 Welborn Street Dallas, TX 75219 Tel: 214-559-7766 fax: 214-559-7768 From Richard.Cartun <@t> hhchealth.org Mon Dec 16 17:00:16 2013 From: Richard.Cartun <@t> hhchealth.org (Cartun, Richard) Date: Mon Dec 16 17:00:27 2013 Subject: [Histonet] RE: Charges for recuts on legal cases In-Reply-To: References: Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E018A0B13@HHCEXCHMB05.hhcsystem.org> Here in Connecticut we are not allowed to charge for the preparation of pathology slides for legal purposes. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax richard.cartun@hhchealth.org ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Vickroy, Jim [Vickroy.Jim@mhsil.com] Sent: Monday, December 16, 2013 11:16 AM To: histonet@lists.utsouthwestern.edu Cc: Bantner, Diane Subject: [Histonet] Charges for recuts on legal cases Can anyone share with me what they charge for doing recuts on cases that have been pulled for legal purposes? I am trying to see what others charge before we assign a charge. I think most people are probably charging by the slide but please let me know if you charge differently. Thanks James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From ruio7 <@t> hotmail.com Mon Dec 16 23:41:08 2013 From: ruio7 <@t> hotmail.com (Rui TAHARA) Date: Mon Dec 16 23:41:12 2013 Subject: [Histonet] variable staining of alcian blue Message-ID: Hello, I am staining paraffin sections (10 microns) with Alcian blue and Nuclear Fast Red as standard protocol as below. The problem is that I see some sections stained with alicain blue show some variability (hope i am able to attach image); alcian blue stain are washed out in some sections even in the same slide. Those sections show pink color in the cartilage. Clearing dehydrate 1% Alcain blue (PH2.5) 30 mins dip in tap water running water 2mins dip deionized water 0.1% Nuclear Fast red 5 mins dip deionized water 30 sec X 2 95% ethanol 1min 100% ethanol 1 mins X 2 clearing Is this something to do with filtering alcian blue or nuclear fast red? I filtered alcian blue using needles to eliminate a big undisolved particle, but I did not filter the nuclear fast red since it seems all dissolved well. also I rarely had the problem last time i used the same staining. I know i need to filter with filter paper but when i tried, even with non-fine filter paper, it absorbed too much dye and was not successful. I tried to wash nuclear fast red with running tap water 1 min and dip in deionized water intead of twice wash of deionized water as described above. However, variable staining of alcian blue still present. Does anybody have same problem before? I appreciate any suggestion to solve this. Thanks. Rui From TMcNemar <@t> lmhealth.org Tue Dec 17 04:48:28 2013 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Tue Dec 17 04:48:39 2013 Subject: [Histonet] Re: Staining on Alcohol Fixed Smears In-Reply-To: <52AF5383.4080103@gmail.com> References: <52AF5383.4080103@gmail.com> Message-ID: Thanks for the clarification. I appreciate the info! Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Beth Cox Sent: Monday, December 16, 2013 2:25 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Staining on Alcohol Fixed Smears I agree that with no formalin exposure, there is no crosslinking that needs to be broken, but a "then, do nothing" approach to antigen retrieval oversimplifies the issue. Alcohol fixation makes changes in the protiens/antibodies also, although different changes than formalin does, and these changes need to be accounted for. In my research on alcohol fixed smears, I found that most antibodies perform best with some antigen retrieval, often gentler than formalin tissues require. Beth Cox, HTL/SCT(ASCP)QIHC Vagabond Histotech ------------------------------ Message: 4 Date: Mon, 16 Dec 2013 11:13:59 -0500 From: Tom McNemar Subject: RE: [Histonet] Re: Staining on Alcohol Fixed Smears To: 'Beth Cox', "histonet@lists.utsouthwestern.edu" ,"levi.fried@gmail.com" Message-ID: Content-Type: text/plain; charset="us-ascii" Since there is no exposure to formalin, we do not do antigen retrieval on alcohol fixed specimens. We use the Ventana Benchmark XT and copy the protocol to a new number and use the "Wet" option rather than paraffin. No retrieval needed. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From:histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Beth Cox Sent: Saturday, December 14, 2013 6:30 PM To:histonet@lists.utsouthwestern.edu;levi.fried@gmail.com Subject: [Histonet] Re: Staining on Alcohol Fixed Smears Levi, I've worked up most of the more common antibodies on alcohol fixed smears. There are two things to remember here: 1) your specimens are alcohol fixed, meaning antigen retrieval needs are different, and 2) they are smears, meaning the cells weren't 'cut open' during microtomy, which impeeds some nuclear staining. Those are two different issues to consider. Some rules of thumb: 1) most cell membrane antigens will stain nicely with minimal changes to the protocols you use for FFPE specimens 2) most cytokeratin antigens stain nicely with little change to your FFPE protocols (if you need to change, start by cutting your antigen retrieval time in half) 3) most nuclear antigens require significant changes from FFPE protocols, and some are essentially impossible to stain using standard antibodies. When I return to work on Monday, I will forward you my protocol 'adjustments' for the antibodies you requested. Beth Cox, HTL/SCT(ASCP)QIHC Vagabond Histotech On 12/14/2013 12:00 PM,histonet-request@lists.utsouthwestern.edu wrote: > Message: 4 > Date: Sat, 14 Dec 2013 18:25:08 +0200 > From: Levi Fried > Subject: [Histonet] Staining on Alcohol Fixed Smears. > To:histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset=ISO-8859-1 > > Hi Everyone. > > I am looking to stain alcohol fixed smears with a group of antibodies. > > The antibodies of particular interest are p63, p53 and ki67. > Along with these antibodies I am looking for positive nuclear and > cytoplastic staining antibodies. > > If anyone has any experience in staining alcohol fixed slides your > information is very appreciated. > > All the best. > > -- Sincerely, - Levi Fried _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From sforeman <@t> labpath.com Tue Dec 17 08:40:10 2013 From: sforeman <@t> labpath.com (Susan Foreman) Date: Tue Dec 17 08:45:15 2013 Subject: [Histonet] Re: Staining on Alcohol Fixed Smears In-Reply-To: References: <52AF5383.4080103@gmail.com> Message-ID: <000c01cefb35$e5b6e450$b124acf0$@com> What info do you guys have on acetone fixed smears? Specifically, having trouble getting PAX5 (nuclear) to show good signal without chewing up the cells during antigen retrieval. Susan Foreman, HT (ASCP) KDL Pathology 315 Erin Drive Knoxville, TN 37919 (865)584-1933 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Tuesday, December 17, 2013 5:48 AM To: 'Beth Cox'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Staining on Alcohol Fixed Smears Thanks for the clarification. I appreciate the info! Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Beth Cox Sent: Monday, December 16, 2013 2:25 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Staining on Alcohol Fixed Smears I agree that with no formalin exposure, there is no crosslinking that needs to be broken, but a "then, do nothing" approach to antigen retrieval oversimplifies the issue. Alcohol fixation makes changes in the protiens/antibodies also, although different changes than formalin does, and these changes need to be accounted for. In my research on alcohol fixed smears, I found that most antibodies perform best with some antigen retrieval, often gentler than formalin tissues require. Beth Cox, HTL/SCT(ASCP)QIHC Vagabond Histotech ------------------------------ Message: 4 Date: Mon, 16 Dec 2013 11:13:59 -0500 From: Tom McNemar Subject: RE: [Histonet] Re: Staining on Alcohol Fixed Smears To: 'Beth Cox', "histonet@lists.utsouthwestern.edu" ,"levi.fried@gmail.com" Message-ID: Content-Type: text/plain; charset="us-ascii" Since there is no exposure to formalin, we do not do antigen retrieval on alcohol fixed specimens. We use the Ventana Benchmark XT and copy the protocol to a new number and use the "Wet" option rather than paraffin. No retrieval needed. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From:histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Beth Cox Sent: Saturday, December 14, 2013 6:30 PM To:histonet@lists.utsouthwestern.edu;levi.fried@gmail.com Subject: [Histonet] Re: Staining on Alcohol Fixed Smears Levi, I've worked up most of the more common antibodies on alcohol fixed smears. There are two things to remember here: 1) your specimens are alcohol fixed, meaning antigen retrieval needs are different, and 2) they are smears, meaning the cells weren't 'cut open' during microtomy, which impeeds some nuclear staining. Those are two different issues to consider. Some rules of thumb: 1) most cell membrane antigens will stain nicely with minimal changes to the protocols you use for FFPE specimens 2) most cytokeratin antigens stain nicely with little change to your FFPE protocols (if you need to change, start by cutting your antigen retrieval time in half) 3) most nuclear antigens require significant changes from FFPE protocols, and some are essentially impossible to stain using standard antibodies. When I return to work on Monday, I will forward you my protocol 'adjustments' for the antibodies you requested. Beth Cox, HTL/SCT(ASCP)QIHC Vagabond Histotech On 12/14/2013 12:00 PM,histonet-request@lists.utsouthwestern.edu wrote: > Message: 4 > Date: Sat, 14 Dec 2013 18:25:08 +0200 > From: Levi Fried > Subject: [Histonet] Staining on Alcohol Fixed Smears. > To:histonet@lists.utsouthwestern.edu > Message-ID: > > > Content-Type: text/plain; charset=ISO-8859-1 > > Hi Everyone. > > I am looking to stain alcohol fixed smears with a group of antibodies. > > The antibodies of particular interest are p63, p53 and ki67. > Along with these antibodies I am looking for positive nuclear and > cytoplastic staining antibodies. > > If anyone has any experience in staining alcohol fixed slides your > information is very appreciated. > > All the best. > > -- Sincerely, - Levi Fried _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bethcoxx <@t> gmail.com Tue Dec 17 09:19:24 2013 From: bethcoxx <@t> gmail.com (Beth Cox) Date: Tue Dec 17 09:19:34 2013 Subject: [Histonet] Re: Staining on Alcohol Fixed Smears In-Reply-To: <000c01cefb35$e5b6e450$b124acf0$@com> References: <52AF5383.4080103@gmail.com> <000c01cefb35$e5b6e450$b124acf0$@com> Message-ID: <52B06B7C.6070503@gmail.com> I'm sorry, I have no experience with acetone fixed smears. Beth Cox, HTL/SCT(ASCP)QIHC Vagabond Histotech On 12/17/2013 8:40 AM, Susan Foreman wrote: > What info do you guys have on acetone fixed smears? Specifically, having > trouble getting PAX5 (nuclear) to show good signal without chewing up the > cells during antigen retrieval. > > Susan Foreman, HT (ASCP) > KDL Pathology > 315 Erin Drive > Knoxville, TN 37919 > (865)584-1933 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar > Sent: Tuesday, December 17, 2013 5:48 AM > To: 'Beth Cox'; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Re: Staining on Alcohol Fixed Smears > > Thanks for the clarification. I appreciate the info! > > Tom McNemar, HT(ASCP) > Histology Co-ordinator > Licking Memorial Health Systems > (740) 348-4163 > (740) 348-4166 > tmcnemar@lmhealth.org > www.LMHealth.org > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Beth Cox > Sent: Monday, December 16, 2013 2:25 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: Staining on Alcohol Fixed Smears > > I agree that with no formalin exposure, there is no crosslinking that needs > to be broken, but a "then, do nothing" approach to antigen > retrieval oversimplifies the issue. Alcohol fixation makes changes in > the protiens/antibodies also, although different changes than formalin does, > and these changes need to be accounted for. In my research on alcohol fixed > smears, I found that most antibodies perform best with some antigen > retrieval, often gentler than formalin tissues require. > > Beth Cox, HTL/SCT(ASCP)QIHC > Vagabond Histotech > > ------------------------------ > > Message: 4 > Date: Mon, 16 Dec 2013 11:13:59 -0500 > From: Tom McNemar > Subject: RE: [Histonet] Re: Staining on Alcohol Fixed Smears > To: 'Beth Cox', > "histonet@lists.utsouthwestern.edu" > ,"levi.fried@gmail.com" > > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > > Since there is no exposure to formalin, we do not do antigen retrieval on > alcohol fixed specimens. We use the Ventana Benchmark XT and copy the > protocol to a new number and use the "Wet" option rather than paraffin. No > retrieval needed. > > Tom McNemar, HT(ASCP) > Histology Co-ordinator > Licking Memorial Health Systems > (740) 348-4163 > (740) 348-4166 > tmcnemar@lmhealth.org > www.LMHealth.org > > -----Original Message----- > From:histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Beth Cox > Sent: Saturday, December 14, 2013 6:30 PM > To:histonet@lists.utsouthwestern.edu;levi.fried@gmail.com > Subject: [Histonet] Re: Staining on Alcohol Fixed Smears > > Levi, > > I've worked up most of the more common antibodies on alcohol fixed smears. > There are two things to remember here: 1) your specimens are alcohol fixed, > meaning antigen retrieval needs are different, and 2) they are smears, > meaning the cells weren't 'cut open' during microtomy, which impeeds some > nuclear staining. Those are two different issues to consider. > > Some rules of thumb: > 1) most cell membrane antigens will stain nicely with minimal changes to > the protocols you use for FFPE specimens > 2) most cytokeratin antigens stain nicely with little change to your FFPE > protocols (if you need to change, start by cutting your antigen retrieval > time in half) > 3) most nuclear antigens require significant changes from FFPE protocols, > and some are essentially impossible to stain using standard antibodies. > > When I return to work on Monday, I will forward you my protocol > 'adjustments' for the antibodies you requested. > > Beth Cox, HTL/SCT(ASCP)QIHC > Vagabond Histotech > > > > > On 12/14/2013 12:00 PM,histonet-request@lists.utsouthwestern.edu wrote: > >> Message: 4 >> Date: Sat, 14 Dec 2013 18:25:08 +0200 >> From: Levi Fried >> Subject: [Histonet] Staining on Alcohol Fixed Smears. >> To:histonet@lists.utsouthwestern.edu >> Message-ID: >> >> >> Content-Type: text/plain; charset=ISO-8859-1 >> >> Hi Everyone. >> >> I am looking to stain alcohol fixed smears with a group of antibodies. >> >> The antibodies of particular interest are p63, p53 and ki67. >> Along with these antibodies I am looking for positive nuclear and >> cytoplastic staining antibodies. >> >> If anyone has any experience in staining alcohol fixed slides your >> information is very appreciated. >> >> All the best. >> >> -- Sincerely, - Levi Fried > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This e-mail, including attachments, is intended for the sole use of the > individual and/or entity to whom it is addressed, and contains information > from Licking Memorial Health Systems which is confidential or privileged. If > you are not the intended recipient, nor authorized to receive for the > intended recipient, be aware that any disclosure, copying, distribution or > use of the contents of this e-mail and attachments is prohibited. If you > have received this in error, please advise the sender by reply e-mail and > delete the message immediately. You may also contact the LMH Process > Improvement Center at 740-348-4641. E-mail transmissions cannot be > guaranteed to be secure or error-free as information could be intercepted, > corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. > The sender therefore does not accept liability for any errors or omissions > in the contents of this message, which arise as a result of e-mail > transmission. Thank you. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From Nancy_Schmitt <@t> pa-ucl.com Tue Dec 17 11:26:28 2013 From: Nancy_Schmitt <@t> pa-ucl.com (Nancy Schmitt) Date: Tue Dec 17 11:26:33 2013 Subject: [Histonet] slides with flimsy coverslip coming off Message-ID: <906B4DA90ED1DB4DB6C7E94D7CEE6C36815335BE@PEITHA.wad.pa-ucl.com> We received some slides (being used in a school program) - they have flexible plastic coverslips that are coming off and the tissue is adhered to the coverslip. Does anyone have any experience or thoughts on how we might get the tissue to adhere back to the slide? Thank you! Nancy Schmitt HT, MLT(ASCP) Histology Coordinator United Clinical Laboratories NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From nalesnikma <@t> upmc.edu Tue Dec 17 11:38:45 2013 From: nalesnikma <@t> upmc.edu (Nalesnik, Michael) Date: Tue Dec 17 11:38:55 2013 Subject: [Histonet] Paraffin sections or formalin fixed tissue for EM - looking for reference lab Message-ID: <17118600C437C0458A78E63C8E50F512EF0301@MSXMBXNSPRD03.acct.upmchs.net> Hi Carol, We can do that here; I am copying an internal e-mail I got from the pathologist that runs our EM. Hope this helps: Regards, Mike *********** Mike, Can you follow up on this and give me some idea of projected number of cases? I would be interested in talking with Carol Wilson if you have her phone number. Thanks. Sheldon Sheldon Bastacky, MD Director, Nephropathology and Electron Microscopy Laboratory University of Pittsburgh Medical Center - Presbyterian University Hospital Department of Pathology (Room A610) 200 Lothrop Street Pittsburgh, PA 15213-2582 Phone: (412) 647-9612 Fax: (412) 647-3399 Pager: (412) 392-7205 E-mail: bastackysi@upmc.edu https://pathconsult.upmc.com/ ****************** Michael A. Nalesnik, M.D. Professor of Pathology Division of Transplantation and Hepatic Pathology UPMC Montefiore Rm E738 phone 412-647-7645 fax 412-647-5237 Confidential UPMC Health System information. Any unauthorized or improper disclosure, copying, distribution, or use of the contents of this e-mail and attached document(s) is prohibited. The information contained in this e-mail and attached document(s) is intended only for the personal and confidential use of the recipient(s) named above. If you have received this communication in error, please notify the sender immediately by e-mail and delete the original e-mail and attached document(s). By communicating with UPMC staff through e-mail, you agree to comply with UPMC's e-mail terms of use found at >. Should you decide that you do not want to comply with these terms, it is your obligation to reply to those UPMC staff members with whom you are corresponding to indicate you do not agree to comply with these terms and cease further communication with UPMC by e-mail. From foreightl <@t> gmail.com Tue Dec 17 11:40:02 2013 From: foreightl <@t> gmail.com (Patrick Laurie) Date: Tue Dec 17 11:40:06 2013 Subject: [Histonet] slides with flimsy coverslip coming off In-Reply-To: <906B4DA90ED1DB4DB6C7E94D7CEE6C36815335BE@PEITHA.wad.pa-ucl.com> References: <906B4DA90ED1DB4DB6C7E94D7CEE6C36815335BE@PEITHA.wad.pa-ucl.com> Message-ID: This can happen with the older plastic coverslipping material. I have heard that they have since fixed it though. We had several years worth of slides that this happened to, the only way we could reliably fix it was add coverslipping media below the coverslip and put something flat and heavy on top. Since the coverslips dissolve in acetone, we tried several things with attaching the tissue, using acetone to dissolve the coverslip, but it never worked very well. Patrick Laurie(HT)ASCP QIHC Histology Manager Celligent Diagnostics, LLC 101 East W.T. Harris Blvd | Suite 1212 | Charlotte, NC 28262 Work: 704-970-3300 Cell: 704-266-0869 On Tue, Dec 17, 2013 at 12:26 PM, Nancy Schmitt wrote: > We received some slides (being used in a school program) - they have > flexible plastic coverslips that are coming off and the tissue is adhered > to the coverslip. > Does anyone have any experience or thoughts on how we might get the > tissue to adhere back to the slide? > Thank you! > > Nancy Schmitt HT, MLT(ASCP) > Histology Coordinator > United Clinical Laboratories > > > > > NOTICE: This email may contain legally privileged information. The > information > is for the use of only the intended recipient(s) even if addressed > incorrectly. If you are not the intended recipient, please notify the > sender > that you have received it in error and then delete it along with any > attachments. Thank you. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie(HT)ASCP QIHC Histology Manager Celligent Diagnostics, LLC 101 East W.T. Harris Blvd | Suite 1212 | Charlotte, NC 28262 Work: 704-970-3300 Cell: 704-266-0869 From azdudley <@t> hotmail.com Tue Dec 17 11:55:10 2013 From: azdudley <@t> hotmail.com (anita) Date: Tue Dec 17 11:55:17 2013 Subject: [Histonet] grandfathered histotech Message-ID: Just wondering what others have found to be the case with CAP and techs that are grandfathered in. When your labs are inspected is there a problem ? Thanks so much, hope all have a wonderful Christmas!! Anita Dudley From rjbuesa <@t> yahoo.com Tue Dec 17 12:11:10 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Dec 17 12:11:20 2013 Subject: [Histonet] grandfathered histotech In-Reply-To: References: Message-ID: <1387303870.16627.YahooMailNeo@web164902.mail.bf1.yahoo.com> Different states with histotech licenses always provided a "grand-father" clause for those histotechs with years of experience lacking the theoretical training and gran-fathered?them with the license at the same level to those with the studies and without much training. Consequently, a licensed HT is a licensed HT regardless of how they got their license. So the very short answer to your question is: NO! Ren? J. ________________________________ From: anita To: "Histonet@lists.utsouthwestern.edu" Sent: Tuesday, December 17, 2013 12:55 PM Subject: [Histonet] grandfathered histotech Just wondering what others have found to be the case with CAP and techs that are grandfathered in.? When your labs are inspected is there a problem ? Thanks so much, hope all have a wonderful Christmas!! Anita Dudley ??? ??? ??? ? ??? ??? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LLoss <@t> dermwisconsin.com Tue Dec 17 13:03:52 2013 From: LLoss <@t> dermwisconsin.com (Lee Loss) Date: Tue Dec 17 13:03:53 2013 Subject: [Histonet] job postings Message-ID: <2B99CB40EC5CC7409888A2961CBF96884E794F72@EX2010.DERM.LOCAL> Hello all, Where does everyone find the most success when advertising job openings for your histology labs? We currently use Careerbuilder, Monster and also do some advertising with NSH and a few other places. We want to make sure we're hitting the places where the most people are looking. Any thoughts are appreciated. Thanks, Lee Loss Lab Manager Dermatology Associates | Forefront Dermatology lloss@dermwisconsin.com ________________________________ The materials and information in this e-mail are confidential and may contain Protected Health Information covered under the HIPAA Privacy Rule. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution, or action taken in reliance on the contents of this information is strictly forbidden by law. If you have received this e-mail in error, please notify me by reply e-mail and then delete this message. Do not pass any of this information to anyone else. Thank you for your cooperation. From BSullivan <@t> virtua.org Tue Dec 17 13:17:00 2013 From: BSullivan <@t> virtua.org (Sullivan, Beatrice) Date: Tue Dec 17 13:17:08 2013 Subject: [Histonet] RE: job postings In-Reply-To: <2B99CB40EC5CC7409888A2961CBF96884E794F72@EX2010.DERM.LOCAL> References: <2B99CB40EC5CC7409888A2961CBF96884E794F72@EX2010.DERM.LOCAL> Message-ID: <6932520047F7EE46B512E9801344F1607FFEF3FB@ExchangeMB-1.Virtua.org> I found both of my current jobs in ADVANCE magazine. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee Loss Sent: Tuesday, December 17, 2013 2:04 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] job postings Hello all, Where does everyone find the most success when advertising job openings for your histology labs? We currently use Careerbuilder, Monster and also do some advertising with NSH and a few other places. We want to make sure we're hitting the places where the most people are looking. Any thoughts are appreciated. Thanks, Lee Loss Lab Manager Dermatology Associates | Forefront Dermatology lloss@dermwisconsin.com ________________________________ The materials and information in this e-mail are confidential and may contain Protected Health Information covered under the HIPAA Privacy Rule. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution, or action taken in reliance on the contents of this information is strictly forbidden by law. If you have received this e-mail in error, please notify me by reply e-mail and then delete this message. Do not pass any of this information to anyone else. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message, and any included attachments, are from Virtua Health or its related affiliates and is intended only for the addressee(s). The information contained herein is privileged, proprietary or may include confidential information and/or protected patient health information. Any unauthorized review, forwarding, printing, copying, distributing, or otherwise disseminating or taking any action based on such information is strictly prohibited. If you have received this message in error, or have reason to believe you are not authorized to receive it, please delete this message promptly and notify the sender by e-mail with a copy to ISSECURITY@virtua.org. Thank you From POWELL_SA <@t> mercer.edu Tue Dec 17 13:32:08 2013 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Tue Dec 17 13:32:09 2013 Subject: [Histonet] RE: job postings In-Reply-To: <2B99CB40EC5CC7409888A2961CBF96884E794F72@EX2010.DERM.LOCAL> References: <2B99CB40EC5CC7409888A2961CBF96884E794F72@EX2010.DERM.LOCAL> Message-ID: <9BF995BC0E47744E9673A41486E24EE259CBAF1AE7@MERCERMAIL.MercerU.local> www.histosearch.com has a histology job page so that you can list or acquire jobs. Shirley Powell -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee Loss Sent: Tuesday, December 17, 2013 2:04 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] job postings Hello all, Where does everyone find the most success when advertising job openings for your histology labs? We currently use Careerbuilder, Monster and also do some advertising with NSH and a few other places. We want to make sure we're hitting the places where the most people are looking. Any thoughts are appreciated. Thanks, Lee Loss Lab Manager Dermatology Associates | Forefront Dermatology lloss@dermwisconsin.com ________________________________ The materials and information in this e-mail are confidential and may contain Protected Health Information covered under the HIPAA Privacy Rule. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution, or action taken in reliance on the contents of this information is strictly forbidden by law. If you have received this e-mail in error, please notify me by reply e-mail and then delete this message. Do not pass any of this information to anyone else. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From plucas <@t> biopath.org Tue Dec 17 13:45:47 2013 From: plucas <@t> biopath.org (Paula Lucas) Date: Tue Dec 17 13:44:25 2013 Subject: [Histonet] New code 88343 Message-ID: <278BEF80365D4CF88995EC2A3A8B61F6@biopath.local> Hello, Can someone shed some light for me on this new code for IHC? I think the code refers to double stain antibodies? Thanks in advance : ) Paula Lucas Lab Manager Bio-Path Medical Group From lpwenk <@t> sbcglobal.net Tue Dec 17 15:06:31 2013 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Tue Dec 17 15:06:36 2013 Subject: [Histonet] beautiful microscope photos Message-ID: Just for fun. This year?s top 10 Olympus Bioscapes digital imaging competition. Most through microscopes. http://www.wired.com/wiredscience/2013/12/olympus-bioscapes-microscope-photography/#slideid-395121 Peggy A. Wenk, HTL(ASCP)SLS From rheyna <@t> lumc.edu Tue Dec 17 15:10:12 2013 From: rheyna <@t> lumc.edu (Roger Heyna) Date: Tue Dec 17 15:10:21 2013 Subject: [Histonet] Discontinuing Negative Reagent Controls for IHC In-Reply-To: <52A5D7EA020000230008957A@gwgwia1.luhs.org> References: <52A5D7EA020000230008957A@gwgwia1.luhs.org> Message-ID: <52B06954020000230008A178@gwgwia1.luhs.org> Below is the list of Histonet responses representing the labs that have discontinued the use of negative reagent controls for IHC's. Thanks to everyone who responded. Roger Maywood, IL Molecular Diagnostics Lab, Winston-Salem, NC Saint Joseph's Hospital, Atlanta, GA Hartford Hospital, Hartford, CT UC San Francisco Medical Center, San Francisco, CA UCSD Medical Center, San Diego, CA Central Vermont Medical Center, Berlin, VT Beaumont Health System, Royal Oak, MI Saint Francis Medical Center, Cape Girardeau, MO Dominion Pathology Laboratories, Norfolk, VA CSI Laboratories, Alpharetta, GA Unitypoint Health-Methodist, Peoria, IL Rutland Regional Medical Center, Rutland, VT Vanderbilt University, Nashville, TN Physicians Reference Laboratory, LLC, Overland Park, KS Regions Hospital, St. Paul, MN United Clinical Laboratories, Dubuque, IA Holy Redeemer Hospital Laboratory, Meadowbrook, PA St. Luke's Hospital, Duluth, MN PRHS, Naples, FL >>> "Roger Heyna" 12/9/2013 2:47 PM >>> I am putting together a list of facilities that have discontinued the use of negative reagent controls for IHC. After the CAP revised the requirement related to negative controls when polymer-based detection systems are used, we decided to investigate whether discontinuing the negative controls would be possible for our lab. It would be helpful to know what labs have done this successfully. If the labs that have discontinued the use of negatives could just respond in an email, I would appreciate it. Also, if anyone has any thoughts pertaining to this change, they're certainly welcome. Thank you, Roger Maywood, IL From Joyce.Weems <@t> emoryhealthcare.org Tue Dec 17 17:03:02 2013 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Tue Dec 17 17:03:12 2013 Subject: [Histonet] Microtome Service Message-ID: Hello Folks, I know that you know that every time someone mentions Leitz or Leica problems with a microtome or a cryotstat, I send out the name of Klaus Dern, my good friend for so many years I've lost count. I want to let you know about something he can do that save your labs a lot of money, if you want service instead of replacing expensive parts because the one present on the newer microtomes can not be repaired. For the following microtomes, Leica RM2125 Leica 2030 Biocut Leica/Jung 2035 Leica CM-1850 Cryostat Sakura SRM200 The advance mechanism can not be repaired, according to the company. Klaus can retool or whatever you call it, the advance mechanism. Excessive play can develop in the advance mechanism, causing chunking of the block and the agony and gnashing of teeth. The company will tell you the whole part needs to be replaced or perhaps you even need to get a new microtome. Micro Precision Instruments Co (none other than Klaus Dern) can repair the instrument for about half of what the new part would cost. The best thing about the repair is that it becomes adjustable and can forever work with little maintenance. I just wanted to share this with you - I am not the vendor. I just want to let you know of something that can help in these trying times of budget constraints and replacing equipment. He can repair the microtomes out of the cryostats. You can remove and send to him. He has loaners (I asked). I just had a cryostat repaired. He is close enough to me that he can come on site and I don't need to ship. Here is his info for those who might be interested. Phone: 706-635-8840 Fax: 706-635-3074 Klaus.Dern44@gmail.com Hope you find this helpful! Hope everyone is enjoying the hustle and bustle of the season. Merry Christmas, ya'll!! j Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph?s Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From Michael.LaFriniere <@t> ccplab.com Wed Dec 18 06:53:24 2013 From: Michael.LaFriniere <@t> ccplab.com (Michael LaFriniere) Date: Wed Dec 18 06:53:34 2013 Subject: [Histonet] RE: Charges for recuts on legal cases In-Reply-To: <9E2D36CE2D7CBA4A94D9B22E8328A3BA27E7865B35@NADCWPMSGCMS03.hca.corpad.net> References: <9E2D36CE2D7CBA4A94D9B22E8328A3BA27E7865B35@NADCWPMSGCMS03.hca.corpad.net> Message-ID: <4A2A16B9707CE04E9CB6C82DC18C1D2963FED6@AHCMSASEXCH03.my.ahc.local> James I charge about the same as Wanda for legal cases, including an administrative fee to search the case first, sometimes I have the legal firm pay up front prior to sending any slides. I do offer additional slides at $10.00 each Michael Michael R. LaFriniere, HT (ASCP) Executive Director Capital Choice Pathology Laboratory 12041 Bournefield Way, Suite A . Silver Spring, MD 20904 P: 240.471.3427 . F: 240.471.3401 . Cell 410-940-8844 michael.lafriniere@csmlab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Wanda.Smith@HCAhealthcare.com Sent: Monday, December 16, 2013 1:54 PM To: Vickroy.Jim@mhsil.com; histonet@lists.utsouthwestern.edu Cc: Bantner.Diane@mhsil.com Subject: [Histonet] RE: Charges for recuts on legal cases I charge a $30.00 fee for investigating the case and issuing a report. I offer 1 H&E and 2 unstained sections on plus slides and charge an additional $15.00 per slide. They furnish the shipping/FedEx. W WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC? 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Monday, December 16, 2013 11:17 AM To: histonet@lists.utsouthwestern.edu Cc: Bantner, Diane Subject: [Histonet] Charges for recuts on legal cases Can anyone share with me what they charge for doing recuts on cases that have been pulled for legal purposes? I am trying to see what others charge before we assign a charge. I think most people are probably charging by the slide but please let me know if you charge differently. Thanks James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Wed Dec 18 07:07:07 2013 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Wed Dec 18 07:07:12 2013 Subject: [Histonet] Microtome Service In-Reply-To: References: Message-ID: Thanks for this information Joyce. Joelle Weaver MAOM, HTL (ASCP) QIHC From: Joyce.Weems@emoryhealthcare.org To: Histonet@lists.utsouthwestern.edu Date: Tue, 17 Dec 2013 23:03:02 +0000 CC: Subject: [Histonet] Microtome Service Hello Folks, I know that you know that every time someone mentions Leitz or Leica problems with a microtome or a cryotstat, I send out the name of Klaus Dern, my good friend for so many years I've lost count. I want to let you know about something he can do that save your labs a lot of money, if you want service instead of replacing expensive parts because the one present on the newer microtomes can not be repaired. For the following microtomes, Leica RM2125 Leica 2030 Biocut Leica/Jung 2035 Leica CM-1850 Cryostat Sakura SRM200 The advance mechanism can not be repaired, according to the company. Klaus can retool or whatever you call it, the advance mechanism. Excessive play can develop in the advance mechanism, causing chunking of the block and the agony and gnashing of teeth. The company will tell you the whole part needs to be replaced or perhaps you even need to get a new microtome. Micro Precision Instruments Co (none other than Klaus Dern) can repair the instrument for about half of what the new part would cost. The best thing about the repair is that it becomes adjustable and can forever work with little maintenance. I just wanted to share this with you - I am not the vendor. I just want to let you know of something that can help in these trying times of budget constraints and replacing equipment. He can repair the microtomes out of the cryostats. You can remove and send to him. He has loaners (I asked). I just had a cryostat repaired. He is close enough to me that he can come on site and I don't need to ship. Here is his info for those who might be interested. Phone: 706-635-8840 Fax: 706-635-3074 Klaus.Dern44@gmail.com Hope you find this helpful! Hope everyone is enjoying the hustle and bustle of the season. Merry Christmas, ya'll!! j Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph?s Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From benjamin <@t> histologistics.com Wed Dec 18 08:40:52 2013 From: benjamin <@t> histologistics.com (Benjamin) Date: Wed Dec 18 08:41:05 2013 Subject: [Histonet] beautiful microscope photos In-Reply-To: References: Message-ID: <3FE7168F-4895-43A3-A946-C8551D184BAA@histologistics.com> Cool, thank you. Sent from my iPhone > On Dec 17, 2013, at 4:06 PM, "Lee & Peggy Wenk" wrote: > > Just for fun. This year?s top 10 Olympus Bioscapes digital imaging competition. Most through microscopes. > > http://www.wired.com/wiredscience/2013/12/olympus-bioscapes-microscope-photography/#slideid-395121 > > Peggy A. Wenk, HTL(ASCP)SLS > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vtobias <@t> uw.edu Wed Dec 18 10:36:49 2013 From: vtobias <@t> uw.edu (Victor A. Tobias) Date: Wed Dec 18 10:36:59 2013 Subject: [Histonet] RE: Charges for recuts on legal cases In-Reply-To: <4A2A16B9707CE04E9CB6C82DC18C1D2963FED6@AHCMSASEXCH03.my.ahc.local> References: <9E2D36CE2D7CBA4A94D9B22E8328A3BA27E7865B35@NADCWPMSGCMS03.hca.corpad.net> <4A2A16B9707CE04E9CB6C82DC18C1D2963FED6@AHCMSASEXCH03.my.ahc.local> Message-ID: We charge a 24 processing fee, and about 10.50 per he or unstained slide. We also offer special stains for slightly higher fee. If we can't do recuts, we offer whole slide scans for about 23 per slide. If they want documents, it is 1.09 per page the first 30 pgs and lesser fee per pg thereafter. Pathologist review to approve recuts is 30. We charge 11 for shipping if they don't provide fedex acct. WA has statutes and regs capping the amount on what can be charged for documents. Nothing re slides. Victor Tobias HT(ASCP) Clinical Applications Analyst Harborview Medical Center Dept of Pathology Room NJB 244 Ninth & Jefferson Seattle, WA 98104 vtobias@u.washington.edu 206-744-2735 206-744-8240 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael LaFriniere Sent: Wednesday, December 18, 2013 4:53 AM To: wanda.smith@hcahealthcare.com; vickroy.jim@mhsil.com; histonet@lists.utsouthwestern.edu Cc: bantner.diane@mhsil.com Subject: [Histonet] RE: Charges for recuts on legal cases James I charge about the same as Wanda for legal cases, including an administrative fee to search the case first, sometimes I have the legal firm pay up front prior to sending any slides. I do offer additional slides at $10.00 each Michael Michael R. LaFriniere, HT (ASCP) Executive Director Capital Choice Pathology Laboratory 12041 Bournefield Way, Suite A . Silver Spring, MD 20904 P: 240.471.3427 . F: 240.471.3401 . Cell 410-940-8844 michael.lafriniere@csmlab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Wanda.Smith@HCAhealthcare.com Sent: Monday, December 16, 2013 1:54 PM To: Vickroy.Jim@mhsil.com; histonet@lists.utsouthwestern.edu Cc: Bantner.Diane@mhsil.com Subject: [Histonet] RE: Charges for recuts on legal cases I charge a $30.00 fee for investigating the case and issuing a report. I offer 1 H&E and 2 unstained sections on plus slides and charge an additional $15.00 per slide. They furnish the shipping/FedEx. W WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC? 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Monday, December 16, 2013 11:17 AM To: histonet@lists.utsouthwestern.edu Cc: Bantner, Diane Subject: [Histonet] Charges for recuts on legal cases Can anyone share with me what they charge for doing recuts on cases that have been pulled for legal purposes? I am trying to see what others charge before we assign a charge. I think most people are probably charging by the slide but please let me know if you charge differently. Thanks James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sheriblair1 <@t> netzero.net Wed Dec 18 12:24:25 2013 From: sheriblair1 <@t> netzero.net (sheriblair1@netzero.net) Date: Wed Dec 18 12:26:27 2013 Subject: [Histonet] Job listings Message-ID: <20131218.122425.18643.0@webmail08.vgs.untd.com> Check with your state Histology Society. Word of mouth and networking Can go a long way. They may also put an ad in a newsletter or website if they have one. From Sarah.Dysart <@t> stdavids.com Wed Dec 18 13:07:44 2013 From: Sarah.Dysart <@t> stdavids.com (Sarah.Dysart@stdavids.com) Date: Wed Dec 18 13:07:50 2013 Subject: [Histonet] I'm BACK!! Message-ID: <34C22BB94729434598D767D3F4EB95E085BDA94D5C@FWDCWPMSGCMS03.hca.corpad.net> Hello guys and dolls...I'm back!! So not having done CAP in awhile, can someone please direct me where to go to get all the regulations? I tried the CAP website, but didn't see where to go? Maybe I'm being dense? Main thing I am currently working on is validating IHC stains. 10 case slides should be sufficient right? This is after the optimal primary concentration has been established. Thanks Sarah E. Dysart, BA, HT (ASCP), QIHC (ASCP) Pathology Supervisor St. David's North Austin Medical Center 12221 North Mopac Expressway Austin, Tx 78758 (512) 901-1220 From joewalker <@t> rrmc.org Wed Dec 18 15:15:39 2013 From: joewalker <@t> rrmc.org (Joe W. Walker, Jr.) Date: Wed Dec 18 15:15:46 2013 Subject: [Histonet] New code 88343 In-Reply-To: <278BEF80365D4CF88995EC2A3A8B61F6@biopath.local> References: <278BEF80365D4CF88995EC2A3A8B61F6@biopath.local> Message-ID: <3C2378778400AD448ADA6FD6BDB7CCCC181756C7@RRMBX03.rrmc.local> Yes, this appears to be correct. Excerpted from Dennis Padget: AMA prescribes codes 88342 and 88343 for qualitative IHC: The AMA's CPT-2014 codebook offers two codes for reporting with qualitative immunohistochemistry (IHC) testing. The codes and official parenthetical instructions for their use starting Jan. 1 are as follows: 88342 Immunohistochemistry or immunocytochemistry, each separately identifiable antibody per block, cytologic preparation, or hematologic smear; first separately identifiable antibody per slide (Do not report 88342 in conjunction with 88360 or 88361 for the same antibody) (For quantitative or semi-quantitative immunohistochemistry, see 88360, 88361) 88343 each additional separately identifiable antibody per slide (List separately in addition to code for primary procedure) (Use 88343 in conjunction with 88342) (When multiple antibodies are applied to the same slide, use one unit of 88342 for the first separately identifiable antibody and one unit of 88343 for each additional identifiable antibody) CMS prescribes codes G0461 and G0462 for qualitative IHC: CMS will not accept CPT code 88342 or 88343 on a claim effective with dates of service on and after Jan. 1, 2014: Those two codes are "not valid for Medicare purposes" and will be summarily denied if billed. To report a professional, technical or global charge for qualitative immunohistochemistry (IHC) testing for a Medicare beneficiary on and after Jan. 1, 2014, you must use the applicable HCPCS Level II code as follows: G0461 Immunohistochemistry or immunocytochemistry, per specimen; first single or multiplex antibody stain G0462 each additional single or multiplex antibody stain (List separately in addition to code for primary procedure) Codes G0461 and G0462 have 26 and TC modifier lines in the 2014 physician fee schedule, so you'll bill them using the modifier (or no modifier, if entitled to bill the global service) that applies to your practice and any given Medicare beneficiary claim. CMS prescribes that you continue to bill for qualitative IHC testing 'per specimen' as you have since Jan. 1, 2012. Furthermore, you'll continue to bill for quantitative IHC testing 'per specimen' using CPT codes 88360 and 88361 just as you do today and have done since Jan. 1, 2012. Hope this helps. We are still working through how we are going to accomplish this in our LIS, Joe W. Walker, Jr. MS, SCT(ASCP)CM Manager of Anatomical Pathology, Microbiology and Reference Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P: 802.747.1790 F: 802.747.6525 Email joewalker@rrmc.org www.rrmc.org Our Vision: To be the Best Community Healthcare System in New England Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet Recognition? and the Governor's Award for Performance Excellence -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Lucas Sent: Tuesday, December 17, 2013 2:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New code 88343 Hello, Can someone shed some light for me on this new code for IHC? I think the code refers to double stain antibodies? Thanks in advance : ) Paula Lucas Lab Manager Bio-Path Medical Group _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. Thank You From sforeman <@t> labpath.com Thu Dec 19 11:37:52 2013 From: sforeman <@t> labpath.com (Susan Foreman) Date: Thu Dec 19 11:43:02 2013 Subject: [Histonet] Counterstain on Ventana Benchmark Ultra Message-ID: <000e01cefce1$0b2d91a0$2188b4e0$@com> Merry Christmas to all those out there in Histoland! How are other labs handling the counterstain for IHC/ICC stained slides on the Ventana Benchmark Ultra? Has anyone figured out how to counterstain online without purchasing Hematoxylin and Bluing directly from Ventana? Any sort of prep kit ? Our slides are beautiful, just hoping to reduce our cost/test. Many Thanks, Susan Foreman, HT (ASCP) (865)661-4734 (865)584-1933 From TGoins <@t> mt.gov Thu Dec 19 11:56:47 2013 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Thu Dec 19 11:56:59 2013 Subject: [Histonet] Counterstain on Ventana Benchmark Ultra In-Reply-To: <000e01cefce1$0b2d91a0$2188b4e0$@com> References: <000e01cefce1$0b2d91a0$2188b4e0$@com> Message-ID: We manually stain, but may switch from hematoxylin to Acid Blue 19 to increase contrast with the red chromogen preferred by the pathologists. Zehntner et al 2008. Synergistic tissue counterstaining and image segmentation techniques for accurate, quantitative immunohistochemistry. Journal of Histochemistry and Cytochemistry 56(10):873-880. Tresa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Susan Foreman Sent: Thursday, December 19, 2013 10:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Counterstain on Ventana Benchmark Ultra Merry Christmas to all those out there in Histoland! How are other labs handling the counterstain for IHC/ICC stained slides on the Ventana Benchmark Ultra? Has anyone figured out how to counterstain online without purchasing Hematoxylin and Bluing directly from Ventana? Any sort of prep kit ? Our slides are beautiful, just hoping to reduce our cost/test. Many Thanks, Susan Foreman, HT (ASCP) (865)661-4734 (865)584-1933 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kim.tournear <@t> yahoo.com Thu Dec 19 12:17:30 2013 From: kim.tournear <@t> yahoo.com (Kim Tournear) Date: Thu Dec 19 12:17:39 2013 Subject: [Histonet] Counterstain on Ventana Benchmark Ultra In-Reply-To: <000e01cefce1$0b2d91a0$2188b4e0$@com> References: <000e01cefce1$0b2d91a0$2188b4e0$@com> Message-ID: <0458B775-CC35-451C-A946-C12AFD543438@yahoo.com> They offer counter stain dispensers you can fill yourself. Sent from the iPhone of Kim Tournear ?? ? On Dec 19, 2013, at 10:37 AM, "Susan Foreman" wrote: > Merry Christmas to all those out there in Histoland! > > > > How are other labs handling the counterstain for IHC/ICC stained slides on > the Ventana Benchmark Ultra? Has anyone figured out how to counterstain > online without purchasing Hematoxylin and Bluing directly from Ventana? Any > sort of prep kit ? Our slides are beautiful, just hoping to reduce our > cost/test. > > > > Many Thanks, > > > > Susan Foreman, HT (ASCP) > (865)661-4734 > > (865)584-1933 > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Robert-Eytalis <@t> RiversideHealthCare.net Thu Dec 19 13:23:51 2013 From: Robert-Eytalis <@t> RiversideHealthCare.net (Eytalis, Robert A) Date: Thu Dec 19 13:23:55 2013 Subject: [Histonet] Looking for a Histo Tech Just south of Chicago Message-ID: http://www.riversidehealthcare.org/employment/openings.html We are in the process of upgrading to a FT position Robert A. Eytalis Laboratory Manager robert-eytalis@riversidehealthcare.net Phone: (815) 935-7256 ext. 5186 (815) 935-7535 Fax (815) 935-7068 Riverside Medical Center 350 N. Wall Street - Kankakee, IL 60901 http://mail.riversidehealthcare.net/owa/redir.aspx?C=qIzWpCGMNkq2SuiIt1v_2X7GmC1aOtAI7VlOiEQ974hhCQzwyDH6yJklmjoBIK92OWDFwivhIZs.&URL=http%3a%2f%2fwww.riversidemc.net%2f | http://mail.riversidehealthcare.net/owa/redir.aspx?C=qIzWpCGMNkq2SuiIt1v_2X7GmC1aOtAI7VlOiEQ974hhCQzwyDH6yJklmjoBIK92OWDFwivhIZs.&URL=http%3a%2f%2fwww.facebook.com%2fRiversideMC From bszpunar <@t> umail.iu.edu Thu Dec 19 20:38:29 2013 From: bszpunar <@t> umail.iu.edu (Bryan Szpunar) Date: Thu Dec 19 20:38:35 2013 Subject: [Histonet] I'm BACK!! Message-ID: Hi Sarah, Here is the 2014 AP checklist question covering your question. Note the part at the end about ER, PR, HER2 in case you are doing those. I can post that as well if it's applicable. ANP.22750 Antibody Validation Phase II The laboratory has documented validation of new antibodies, prior to use in patient diagnosis. NOTE: The performance characteristics of each assay in the immunohistochemistry laboratory must be appropriately validated before being placed into clinical use. The initial goal is to establish the optimal antibody titration, detection system, and antigen retrieval protocol. Once optimized, a panel of tissues must be tested to determine the assay's sensitivity and specificity. The scope of the validation is at the discretion of the laboratory director and will vary with the antibody. For a well-characterized antibody with a limited spectrum of antigenic targets, like chromogranin or prostate specific antigen, the validation can be limited. A panel of 10 positive and 10 negative neoplasms would be sufficient in this setting. For an antibody that is not well characterized and/or has a wide range of reported reactivity, a more extensive validation is necessary. The number of tissues tested should, in this circumstance, be large enough to determine whether the staining profile matches that previously described. An exception to the above requirements is that studies may not be feasible for antigens such as ALK that are only seen in rare tumors. This checklist has additional validation requirements for HER2 and estrogen/progesterone receptor testing in breast carcinoma. Please refer to the subsection "Predictive Markers," below. Evidence of Compliance: ? Written procedure for the evaluation/validation of new antibodies Hope that helps! Bryan Szpunar, HT(ASCP) Histology Lead LabCorp Indianapolis 7750 Zionsville Road Indianapolis, IN 46268 From mpence <@t> grhs.net Fri Dec 20 09:03:12 2013 From: mpence <@t> grhs.net (Mike Pence) Date: Fri Dec 20 09:03:39 2013 Subject: [Histonet] tea bags Message-ID: Hey all, Where is everyone getting their tea bags for filtering endo specimens? I cannot find the company that we were using for our bags. They are paper and measure 2 ? x 2 ? and are sealed on three sides. They come in a strip of bags all attached to each other on one side and about 100 to a ziplock bag. Thanks in advance for the info, Mike Pence GRHS AP Supervisor From turkekul <@t> gmail.com Fri Dec 20 11:02:12 2013 From: turkekul <@t> gmail.com (Mesru T) Date: Fri Dec 20 11:02:18 2013 Subject: [Histonet] Re: Histonet Digest, Vol 121, Issue 23 In-Reply-To: <52b1e2c7.463cb60a.647b.41d2SMTPIN_ADDED_MISSING@mx.google.com> References: <52b1e2c7.463cb60a.647b.41d2SMTPIN_ADDED_MISSING@mx.google.com> Message-ID: Dear Histonetters, I wish you happy holidays and also would like to know if anybody has good antibody for FFPE rat, mouse or human tissues for FOXO1 or FOXO3 ( also pFOXO1 or pFOXO3 would work)? Thanks, Mes From sj_pan <@t> yahoo.com Fri Dec 20 12:09:35 2013 From: sj_pan <@t> yahoo.com (Shirley Pan) Date: Fri Dec 20 12:12:24 2013 Subject: [Histonet] (no subject) Message-ID: <1387562975.35381.YahooMailNeo@web163102.mail.bf1.yahoo.com> Opening for a Full Time Histologist Embedding, Sectioning, IHC and Special Stains for out patient clinic 4:30 AM to 1:00 PM? M - F Palo Alto Medical Foundation Palo Alto, CA 94301 Contact Shirley 650-853-6877 or pans1@pamf.org From lpwenk <@t> sbcglobal.net Fri Dec 20 12:49:19 2013 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Fri Dec 20 12:49:21 2013 Subject: [Histonet] how to unsubscribe for holidays (or forever) Message-ID: <3B0878677C384B1484D4BEF510F5FBC5@HP2010> If you are not going to be at your computer, please ?unsubscribe? from Histonet, as we do not want to be receiving a lot of emails saying ?I?m away from work for the next week?. Go to the bottom of any email from Histonet. - click on the link to the website (http:// . . . ) - go to the bottom of that page, to ?Histonet subscribers? - Under there, go to the second (which makes it the last) sentence, on how to unsubscribe. - type in your email, and then hit the button to unsubscribe. There might be something else you have to do, I don?t remember, and I don?t want to unsubscribe. If you want to permanently unsubscribe from Histonet, that should be it. If you want to re-subscribe when you return, Google Histonet and subscribe, and you can find the page Peggy A. Wenk, HTL(ASCP)SLS From lcolbert <@t> pathmdlabs.com Fri Dec 20 14:28:23 2013 From: lcolbert <@t> pathmdlabs.com (Laurie Colbert) Date: Fri Dec 20 14:29:33 2013 Subject: [Histonet] HistoWrap Filter Paper Message-ID: <12ECD7346266D74691EC2BFC75285E452F3F662C@BFL323E10.pathmdlabs.local> Is there anyone out there who uses the HistoWrap filter paper? I have always ordered them from Obex Industries, and I was wondering if there was another distributor for the product. The HistoWraps are round filter papers that are basically the same type of paper as a lens paper for wrapping small specimens/curettings. The wraps can be folded to fit inside of a plastic funnel and specimens can be filtered directly into the paper. If there is similar alternative that is available, I would be interested in hearing what that is. Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab From aj.taylor <@t> blueyonder.co.uk Fri Dec 20 16:04:13 2013 From: aj.taylor <@t> blueyonder.co.uk (Alan Taylor) Date: Fri Dec 20 15:53:18 2013 Subject: [Histonet] tea bags References: Message-ID: <53E8E23852E34EF2B75E15240221350A@merlin> Hi Mike. We have used teabags that we have obtained from a company in the United States. They are 2.5" x 2.5", sealed on three sides and are perfect for filtering cellular material in preparation for processing. The Company is called Sunburst Bottle, 4200 Commerce Court, Suite 206, Lisle, IL. 60532. Tel: (877)9254500 Bags 2.5" x 2.5" Bleached Paper. 500 $0.06ea. 5000 $0.05ea. They do provide samples for evaluation. Hope this is helpful. Alan Alan Taylor BSc(Hons). FRMS. Microtechnical Services 71 Sweetbrier Lane Heavitree Exeter. Devon. EX1 3AJ. UK www.microtechnicalservices.co.uk ----- Original Message ----- From: "Mike Pence" To: ; Sent: Friday, December 20, 2013 3:03 PM Subject: [Histonet] tea bags Hey all, Where is everyone getting their tea bags for filtering endo specimens? I cannot find the company that we were using for our bags. They are paper and measure 2 ? x 2 ? and are sealed on three sides. They come in a strip of bags all attached to each other on one side and about 100 to a ziplock bag. Thanks in advance for the info, Mike Pence GRHS AP Supervisor _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DSiena <@t> statlab.com Fri Dec 20 15:59:04 2013 From: DSiena <@t> statlab.com (Debra Siena) Date: Fri Dec 20 15:59:08 2013 Subject: [Histonet] RE: tea bags In-Reply-To: References: Message-ID: StatLab Medical Products sells the paper tea bags, you can contact them at 800-442-3573. Thank you ? Debbie Siena, HT(ASCP)QIHC StatLab Medical Products Technical Support Manager 407 Interchange Street | McKinney, TX 75071 t:?800.442.3573 ext. 229 | f: 972.436.1369 dsiena@statlab.com | www.statlab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Friday, December 20, 2013 9:03 AM To: histonet-bounces@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] tea bags Hey all, Where is everyone getting their tea bags for filtering endo specimens? I cannot find the company that we were using for our bags. They are paper and measure 2 ? x 2 ? and are sealed on three sides. They come in a strip of bags all attached to each other on one side and about 100 to a ziplock bag. Thanks in advance for the info, Mike Pence GRHS AP Supervisor _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HornHV <@t> archildrens.org Fri Dec 20 16:03:42 2013 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Fri Dec 20 16:04:00 2013 Subject: [Histonet] tea bags In-Reply-To: <53E8E23852E34EF2B75E15240221350A@merlin> References: <53E8E23852E34EF2B75E15240221350A@merlin> Message-ID: <25A4DE08332B19499904459F00AAACB719DCC98E8B@EVS1.archildrens.org> We use the same as Mike. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Alan Taylor Sent: Friday, December 20, 2013 4:04 PM To: Mike Pence; histonet-bounces@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] tea bags Hi Mike. We have used teabags that we have obtained from a company in the United States. They are 2.5" x 2.5", sealed on three sides and are perfect for filtering cellular material in preparation for processing. The Company is called Sunburst Bottle, 4200 Commerce Court, Suite 206, Lisle, IL. 60532. Tel: (877)9254500 Bags 2.5" x 2.5" Bleached Paper. 500 $0.06ea. 5000 $0.05ea. They do provide samples for evaluation. Hope this is helpful. Alan Alan Taylor BSc(Hons). FRMS. Microtechnical Services 71 Sweetbrier Lane Heavitree Exeter. Devon. EX1 3AJ. UK www.microtechnicalservices.co.uk ----- Original Message ----- From: "Mike Pence" To: ; Sent: Friday, December 20, 2013 3:03 PM Subject: [Histonet] tea bags Hey all, Where is everyone getting their tea bags for filtering endo specimens? I cannot find the company that we were using for our bags. They are paper and measure 2 ? x 2 ? and are sealed on three sides. They come in a strip of bags all attached to each other on one side and about 100 to a ziplock bag. Thanks in advance for the info, Mike Pence GRHS AP Supervisor _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. 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From tissuetech <@t> juno.com Sun Dec 22 19:36:35 2013 From: tissuetech <@t> juno.com (tissuetech@juno.com) Date: Sun Dec 22 19:38:06 2013 Subject: Fw: [Histonet] Histology Accessioning Position Open Message-ID: <20131222.193635.30372.0@webmail08.vgs.untd.com> ---------- Forwarded Message ---------- From: "tissuetech@juno.com" To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histology Accessioning Position Open Date: Thu, 6 Jun 2013 18:38:53 GMT Tissue Techniques is a small energetic laboratory with a family feel, and we are currently in search of an experienced Histology Data Entry team member. Tissue Techniques is located in Dallas, Texas. Applying future team members must have knowledge of common Histology terminology, and familiarity of Patient Demographic entry. Please send all interests to tissuetech@juno.com with a subject line of Accessioning Position. Thank you The Tissue Techniques Team ____________________________________________________________ BlackBerry® 10 Find out more about the new BlackBerry 10 smartphone. http://thirdpartyoffers.juno.com/TGL3141/51b0d74e363e9574d5eb6st03vuc _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sforeman <@t> labpath.com Mon Dec 23 08:50:11 2013 From: sforeman <@t> labpath.com (Susan Foreman) Date: Mon Dec 23 08:55:22 2013 Subject: [Histonet] Amphyl vs Lysol IC Message-ID: <000f01ceffee$487a0a60$d96e1f20$@com> Happy Monday, Histonetters. What is your preference for disinfectant solutions? We currently use Amphyl hospital disinfectant, but I see that it is being discontinued. Is the Lysol IC comparable or do you recommend another brand of cleaner? We use the Ventana Benchmark Ultra for our IHC. Any comments are appreciated. Many Thanks, Susan Foreman, HT (ASCP) (865)584-1933 From levi.fried <@t> gmail.com Mon Dec 23 10:45:25 2013 From: levi.fried <@t> gmail.com (Levi Fried) Date: Mon Dec 23 10:45:49 2013 Subject: [Histonet] Antibodies for Ethanol Fixed Smears Message-ID: Hello Everyone. I was wondering if anyone has used the following antibodies on ethanol fixed smears. HSV1 HSV2 p53 ki67 CHK2 EGFR I am looking for specific antibodies used. The ones we are using do not stain well. It would also be great is someone can suggest some dilutions for thos antibodies. Thank you. -- Sincerely, - Levi Fried From andreahooper <@t> rocketmail.com Mon Dec 23 11:33:25 2013 From: andreahooper <@t> rocketmail.com (Andrea Hooper) Date: Mon Dec 23 11:33:40 2013 Subject: [Histonet] CD4 and CD8 background in mouse tumors frozen sections, help In-Reply-To: <8B946A68A8F3534A99CC493DEFB49B101763C875@WM10005P.oneabbott.com> References: <8B946A68A8F3534A99CC493DEFB49B101763C875@WM10005P.oneabbott.com> Message-ID: <7E4904A1-62A5-400A-AAE2-C0A8BA28FB1A@rocketmail.com> Were these samples treated with an antibody based therapeutic and your secondary is detecting the therapeutic? If that's the case and they were treated with a human IgG biologic you will need to use a secondary cross adsorbed against human IgG. Andrea > On Dec 8, 2013, at 4:41 PM, "Erickson, Jamie E" wrote: > > Hello Histonetters, > First I would like to say happy holidays to you all, this is such a great resource to have. > Now for my question. > I'm rather vexed about this problem with staining CD4/Cd8 in mouse tumors I hope someone can shed light on because I'm stumped. > An investigator gave me some frozen mouse tumors and spleen to do CD4 and CD8 stain on and I expected this to be easy... > After staining the spleens and tumor with CD4 and CD8 at (1ug/ml and 5ug/ml respectively) I saw the problem, the isotype control. The isotype for these 2 Rat-mouse antibodies from BD biosciences is a Rat IgG2a. > The rat IgG2a on the mouse tumor and spleen slide were negative and I was happy but when I looked at the other tumors Rat IgG2a slide some were very positive. The CD4 was staining in some tumors but these tumors had background throughout the tissue as well. Very strange I thought, some tumors no background others lots of background.. > > As I'm not a tumor biologist I'll just say these tumors vary in size from small to large, large (>1cm) and some have necrosis others do not. > So what I see is that the isotype (rat IgG2a) stained in some tumors but not all of them and the spleen and a few small tumors are negative. I only did Neg controls on 2 tumors one large and one small tumor with a spleen from that tumor bearing mouse. > > I first thought peroxidase or Fc binding as I don't do a protein blocking step with this system, typically. I added a protein block (Dako) and increase peroxidase time from 5 minute to 10 but saw no difference. > It looked like my titers were high for these tumors so I titered the antibody out. I titered my CD4 down to 0.25ug/ml for these tumors and that did not help the Rat IgG2a staining but the CD4 staining looked better not as dark brown in the spleen. > So now I need to explain my staining protocol.. We use a Leica Bond RX system which I use a DAB kit to detect with and it works great. > > Marker (antibody, Rat Anti-mouse CD4) 15 > Rabbit Anti-Rat > vector lab (adsorbed to mouse) > as a linking step. 20 > polymer (Anti-Rabbit) 8 > Peroxide block 5 > DAB 10 > Hematoxylin 5 > > I next looked at the linker step > slide #1 I did a slide with no CD4.... still had staining (background) > Slide #2 No CD4 and No linker ........No background.. clean....I think it is the linker.. > Unfortunately I'm stuck using this linker (rabbit anti-Rat , 2ug/ml) for now.. > > What could be going on in these tumors ??? could I block this without changing my polymer (rabbit). If it is the linker then is it binding via Rabbit Fc or is the hypervariable region specific for something in these tumors i.e.... Anti-Rat tumor protein? > Can I pre-complex the CD4 and linker then bind up excess anti-Rat, any one done that??? > If it is not complicated enough the linker is adsorbed to mouse. > I tried Biocares Rat-on mouse polymer but I did not see any reaction at all, ..but I haven't investigated that fully.. > > Sorry for the long email .. > Hope you can help...Happy holidays. > > Jamie Erickson > AbbVie BioResearch Center > Pharmacology > 100 Research Drive > Worcester, MA 01605 > OFFICE +1 508-688-3749 > EMAIL terry.melim@abbvie.com > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ihcman2010 <@t> hotmail.com Thu Dec 26 09:11:24 2013 From: ihcman2010 <@t> hotmail.com (Glen Dawson) Date: Thu Dec 26 09:11:28 2013 Subject: [Histonet] 55 gallon drums of formalin In-Reply-To: <7E4904A1-62A5-400A-AAE2-C0A8BA28FB1A@rocketmail.com> References: <8B946A68A8F3534A99CC493DEFB49B101763C875@WM10005P.oneabbott.com>, <7E4904A1-62A5-400A-AAE2-C0A8BA28FB1A@rocketmail.com> Message-ID: All, Who is your prefferred vendor for 55gallon drums of formalin? Thank-you, Glen Dawson BS, HT(ASCP), QIHC Histology Technical Specialist Mercy Health System Janesville, WI From rjbuesa <@t> yahoo.com Thu Dec 26 09:27:01 2013 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Dec 26 09:27:05 2013 Subject: [Histonet] 55 gallon drums of formalin In-Reply-To: References: <8B946A68A8F3534A99CC493DEFB49B101763C875@WM10005P.oneabbott.com>, <7E4904A1-62A5-400A-AAE2-C0A8BA28FB1A@rocketmail.com> Message-ID: <1388071621.86240.YahooMailNeo@web164902.mail.bf1.yahoo.com> Formalin is formalin is formalin so look for the supplier able to sell it the cheapest and with the lowest S/H BUT I strongly recommend not to buy formalin in bulk to neutralize and later aliquot because it is an extremely dangerous activity. Also do not try to "recycle" formalin. Always buy already aliquoted formalin because your health and that of your staff is really priceless. Try to save money by developing a more efficient work flow and preparing your own reagents and solutions but avoid handling formalin as much as possible. Ren? J. ________________________________ From: Glen Dawson To: Cc: histonet Sent: Thursday, December 26, 2013 10:11 AM Subject: [Histonet] 55 gallon drums of formalin All, Who is your prefferred vendor for 55gallon drums of formalin? Thank-you, Glen Dawson? BS, HT(ASCP), QIHC Histology Technical Specialist Mercy Health System Janesville, WI ??? ??? ??? ? ??? ??? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CJacobs <@t> clinpath.com Thu Dec 26 12:39:13 2013 From: CJacobs <@t> clinpath.com (Christopher Jacobs) Date: Thu Dec 26 12:39:19 2013 Subject: [Histonet] Ventana Labels Message-ID: <0082E20E79ED2245AB1DD78B87CFA10949170B4C@cpd-exchange.ClinPath.local> Histonetters, Like everywhere else, my lab is trying to find ways to save money. One thing we are looking at are the labels/ribbon we use for our Ventana EBAR printers and our Ventana Vantage Zebra printers. Currently, we order labels/printer ribbons for both types of printers directly from Ventana. As one can imagine, we are paying a premium price. Has any out there had any luck finding labels/ribbon cheaper from other sources? Thank you everyone! -CJ CONFIDENTIALITY NOTICE: This message and any attachments are for the sole use of the intended recipient(s). This message is confidential and may also be privileged. If you are not the intended recipient, please contact the sender immediately, delete the contents of this message and do not use it for any purpose. From abadesuyi <@t> nrh-ok.com Thu Dec 26 12:53:46 2013 From: abadesuyi <@t> nrh-ok.com (Adesupo, Adesuyi (Banjo)) Date: Thu Dec 26 12:53:49 2013 Subject: [Histonet] LABORATORY SAFETY TEST Message-ID: <04EE4F75BB5FB246ADB68D69B74604438E3B821F66@MAIL.nrhnt.nrh-ok.com> Hi, I am interested in doing the ASCP qualification in laboratory safety test and I will appreciate it, if you guys in histoland could assist/help me with some hints on how to prepare for this test. Thanking you all for your anticipated cooperation. Banjo Adesuyi, BSMT, HT (ASCP), HTL (ASCP), QIHC (ASCP) Histology Supervisor Norman Regional Health System, Norman, OK 73071. Tel: 405- 307- 1145 ====================================== CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. From SSCALISE <@t> beaumont.edu Thu Dec 26 12:59:18 2013 From: SSCALISE <@t> beaumont.edu (Sharon Scalise) Date: Thu Dec 26 12:59:24 2013 Subject: [Histonet] RE: Ventana Labels In-Reply-To: <0082E20E79ED2245AB1DD78B87CFA10949170B4C@cpd-exchange.ClinPath.local> References: <0082E20E79ED2245AB1DD78B87CFA10949170B4C@cpd-exchange.ClinPath.local> Message-ID: <190D98228ADC1747BCE27019B78FD8F3011B3B10@EXMAIL06.ms.beaumont.edu> We order our Vantage printer labels form Eastern Laboratory Services Division and save about $50.00/ roll of labels and ribbon (you get 4 rolls and 4 ribbons per case). You need to be specific with the label measurements and the type of ribbon, otherwise they send standard options. If anyone has anything better, please pass it on! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Christopher Jacobs Sent: Thursday, December 26, 2013 1:39 PM To: 'histonet@lists.utsouthwestern.edu' Cc: Irene G. Campbell, HT (ASCP) Subject: [Histonet] Ventana Labels Histonetters, Like everywhere else, my lab is trying to find ways to save money. One thing we are looking at are the labels/ribbon we use for our Ventana EBAR printers and our Ventana Vantage Zebra printers. Currently, we order labels/printer ribbons for both types of printers directly from Ventana. As one can imagine, we are paying a premium price. Has any out there had any luck finding labels/ribbon cheaper from other sources? Thank you everyone! -CJ CONFIDENTIALITY NOTICE: This message and any attachments are for the sole use of the intended recipient(s). This message is confidential and may also be privileged. If you are not the intended recipient, please contact the sender immediately, delete the contents of this message and do not use it for any purpose. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Donna.Willis <@t> baylorhealth.edu Thu Dec 26 13:03:58 2013 From: Donna.Willis <@t> baylorhealth.edu (Willis, Donna G.) Date: Thu Dec 26 13:04:17 2013 Subject: [Histonet] RE: Ventana Labels In-Reply-To: <190D98228ADC1747BCE27019B78FD8F3011B3B10@EXMAIL06.ms.beaumont.edu> References: <0082E20E79ED2245AB1DD78B87CFA10949170B4C@cpd-exchange.ClinPath.local> <190D98228ADC1747BCE27019B78FD8F3011B3B10@EXMAIL06.ms.beaumont.edu> Message-ID: <2572B4D63B62E64A8078D8BBE34D407801A77E0A@BHDASVEXML2.bhcs.pvt> Sharon, What type of coverslipper are you using? We are having problems with our Leica coverslipper with the Vantage labels. Thanks, Donna Willis, HT/HTL (ASCP) Anatomic Pathology Manager Baylor University Medical Center-Dallas ph. 214-820-2465 office ph. 214-725-6184 mobile donna.willis@baylorhealth.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sharon Scalise Sent: Thursday, December 26, 2013 12:59 PM To: Christopher Jacobs; 'histonet@lists.utsouthwestern.edu' Cc: Irene G. Campbell, HT (ASCP) Subject: [Histonet] RE: Ventana Labels We order our Vantage printer labels form Eastern Laboratory Services Division and save about $50.00/ roll of labels and ribbon (you get 4 rolls and 4 ribbons per case). You need to be specific with the label measurements and the type of ribbon, otherwise they send standard options. If anyone has anything better, please pass it on! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Christopher Jacobs Sent: Thursday, December 26, 2013 1:39 PM To: 'histonet@lists.utsouthwestern.edu' Cc: Irene G. Campbell, HT (ASCP) Subject: [Histonet] Ventana Labels Histonetters, Like everywhere else, my lab is trying to find ways to save money. One thing we are looking at are the labels/ribbon we use for our Ventana EBAR printers and our Ventana Vantage Zebra printers. Currently, we order labels/printer ribbons for both types of printers directly from Ventana. As one can imagine, we are paying a premium price. Has any out there had any luck finding labels/ribbon cheaper from other sources? Thank you everyone! -CJ CONFIDENTIALITY NOTICE: This message and any attachments are for the sole use of the intended recipient(s). This message is confidential and may also be privileged. If you are not the intended recipient, please contact the sender immediately, delete the contents of this message and do not use it for any purpose. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v1/url?u=http://lists.utsouthwestern.edu/mailman/listinfo/histonet&k=eEbcMSXJ22m%2Bwpc%2B3zaDpA%3D%3D%0A&r=tcwXtHSgC9tIe4wFOXToKDTqAni3dMiqUwIdlw6Tfz0%3D%0A&m=lfc%2BC7lvEekkS53jX2GqjpVdam5sUVk4%2BAd5jIzgY3Q%3D%0A&s=2646cd5f80e05975fbb53e90b6e542a90d98307c5c3c6ebb4cc4cd8b6c9d7d3b _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v1/url?u=http://lists.utsouthwestern.edu/mailman/listinfo/histonet&k=eEbcMSXJ22m%2Bwpc%2B3zaDpA%3D%3D%0A&r=tcwXtHSgC9tIe4wFOXToKDTqAni3dMiqUwIdlw6Tfz0%3D%0A&m=lfc%2BC7lvEekkS53jX2GqjpVdam5sUVk4%2BAd5jIzgY3Q%3D%0A&s=2646cd5f80e05975fbb53e90b6e542a90d98307c5c3c6ebb4cc4cd8b6c9d7d3b ********************************************************************** This e-mail may contain confidential and/or privileged information. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. From Timothy.Morken <@t> ucsfmedctr.org Thu Dec 26 13:34:14 2013 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Thu Dec 26 13:34:28 2013 Subject: [Histonet] RE: Ventana Labels In-Reply-To: <2572B4D63B62E64A8078D8BBE34D407801A77E0A@BHDASVEXML2.bhcs.pvt> References: <0082E20E79ED2245AB1DD78B87CFA10949170B4C@cpd-exchange.ClinPath.local> <190D98228ADC1747BCE27019B78FD8F3011B3B10@EXMAIL06.ms.beaumont.edu> <2572B4D63B62E64A8078D8BBE34D407801A77E0A@BHDASVEXML2.bhcs.pvt> Message-ID: <761E2B5697F795489C8710BCC72141FF0BB3AE@ex07.net.ucsf.edu> We use the Sakura Glas (original) with the Ventana Ultra slide labels. It uses a suction cup to grab the slide and works fine. But the Sakura Glas 2 grabs the slide with pincers on the side, like the Leica coverslipper (we use the Leica CV5030)and the Ventana slide labels sometimes get stuck to the pincer. The problem is that the Ultra label is 23mm wide and if the label is right on the edge or hangs over the edge it sticks to the coverslipper pincer. So, you have to place the label very carefully to avoid that problem. Be sure also that the solvent level in the coverslipper is below the label so glue does not seep out (note that I'm not sure if the Ventana Vantage tracking system labels are the same as the Ultra labels). For our tracking system (Copath AB&T) and Leica Bond stainer we are using the 22mm General Data Stainer Shield label and there are no problems at all with either coverslipper. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Willis, Donna G. Sent: Thursday, December 26, 2013 11:04 AM To: 'Sharon Scalise'; Christopher Jacobs; 'histonet@lists.utsouthwestern.edu' Cc: Irene G. Campbell, HT (ASCP) Subject: [Histonet] RE: Ventana Labels Sharon, What type of coverslipper are you using? We are having problems with our Leica coverslipper with the Vantage labels. Thanks, Donna Willis, HT/HTL (ASCP) Anatomic Pathology Manager Baylor University Medical Center-Dallas ph. 214-820-2465 office ph. 214-725-6184 mobile donna.willis@baylorhealth.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sharon Scalise Sent: Thursday, December 26, 2013 12:59 PM To: Christopher Jacobs; 'histonet@lists.utsouthwestern.edu' Cc: Irene G. Campbell, HT (ASCP) Subject: [Histonet] RE: Ventana Labels We order our Vantage printer labels form Eastern Laboratory Services Division and save about $50.00/ roll of labels and ribbon (you get 4 rolls and 4 ribbons per case). You need to be specific with the label measurements and the type of ribbon, otherwise they send standard options. If anyone has anything better, please pass it on! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Christopher Jacobs Sent: Thursday, December 26, 2013 1:39 PM To: 'histonet@lists.utsouthwestern.edu' Cc: Irene G. Campbell, HT (ASCP) Subject: [Histonet] Ventana Labels Histonetters, Like everywhere else, my lab is trying to find ways to save money. One thing we are looking at are the labels/ribbon we use for our Ventana EBAR printers and our Ventana Vantage Zebra printers. Currently, we order labels/printer ribbons for both types of printers directly from Ventana. As one can imagine, we are paying a premium price. Has any out there had any luck finding labels/ribbon cheaper from other sources? Thank you everyone! -CJ CONFIDENTIALITY NOTICE: This message and any attachments are for the sole use of the intended recipient(s). This message is confidential and may also be privileged. If you are not the intended recipient, please contact the sender immediately, delete the contents of this message and do not use it for any purpose. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v1/url?u=http://lists.utsouthwestern.edu/mailman/listinfo/histonet&k=eEbcMSXJ22m%2Bwpc%2B3zaDpA%3D%3D%0A&r=tcwXtHSgC9tIe4wFOXToKDTqAni3dMiqUwIdlw6Tfz0%3D%0A&m=lfc%2BC7lvEekkS53jX2GqjpVdam5sUVk4%2BAd5jIzgY3Q%3D%0A&s=2646cd5f80e05975fbb53e90b6e542a90d98307c5c3c6ebb4cc4cd8b6c9d7d3b _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v1/url?u=http://lists.utsouthwestern.edu/mailman/listinfo/histonet&k=eEbcMSXJ22m%2Bwpc%2B3zaDpA%3D%3D%0A&r=tcwXtHSgC9tIe4wFOXToKDTqAni3dMiqUwIdlw6Tfz0%3D%0A&m=lfc%2BC7lvEekkS53jX2GqjpVdam5sUVk4%2BAd5jIzgY3Q%3D%0A&s=2646cd5f80e05975fbb53e90b6e542a90d98307c5c3c6ebb4cc4cd8b6c9d7d3b ********************************************************************** This e-mail may contain confidential and/or privileged information. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LSebree <@t> uwhealth.org Thu Dec 26 13:41:29 2013 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Thu Dec 26 13:41:35 2013 Subject: [Histonet] RE: Ventana Labels In-Reply-To: <0082E20E79ED2245AB1DD78B87CFA10949170B4C@cpd-exchange.ClinPath.local> References: <0082E20E79ED2245AB1DD78B87CFA10949170B4C@cpd-exchange.ClinPath.local> Message-ID: <77DD817201982748BC67D7960F2F76AF087EFD@UWHC-MBX12.uwhis.hosp.wisc.edu> We tried another vendor for ebar labels, FloPro Tek, easternlabsvc.com, but they didn't hold up in xylene. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Christopher Jacobs Sent: Thursday, December 26, 2013 12:39 PM To: 'histonet@lists.utsouthwestern.edu' Cc: Irene G. Campbell, HT (ASCP) Subject: [Histonet] Ventana Labels Histonetters, Like everywhere else, my lab is trying to find ways to save money. One thing we are looking at are the labels/ribbon we use for our Ventana EBAR printers and our Ventana Vantage Zebra printers. Currently, we order labels/printer ribbons for both types of printers directly from Ventana. As one can imagine, we are paying a premium price. Has any out there had any luck finding labels/ribbon cheaper from other sources? Thank you everyone! -CJ CONFIDENTIALITY NOTICE: This message and any attachments are for the sole use of the intended recipient(s). This message is confidential and may also be privileged. If you are not the intended recipient, please contact the sender immediately, delete the contents of this message and do not use it for any purpose. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SSCALISE <@t> beaumont.edu Thu Dec 26 13:49:56 2013 From: SSCALISE <@t> beaumont.edu (Sharon Scalise) Date: Thu Dec 26 13:50:00 2013 Subject: [Histonet] RE: Ventana Labels In-Reply-To: <77DD817201982748BC67D7960F2F76AF087EFD@UWHC-MBX12.uwhis.hosp.wisc.edu> References: <0082E20E79ED2245AB1DD78B87CFA10949170B4C@cpd-exchange.ClinPath.local> <77DD817201982748BC67D7960F2F76AF087EFD@UWHC-MBX12.uwhis.hosp.wisc.edu> Message-ID: <190D98228ADC1747BCE27019B78FD8F3011B3BAE@EXMAIL06.ms.beaumont.edu> I think you need to order the StainerProTek, we have been using them for months and they do well in all solutions. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A Sent: Thursday, December 26, 2013 2:41 PM To: 'Christopher Jacobs'; 'histonet@lists.utsouthwestern.edu' Cc: Irene G. Campbell, HT (ASCP) Subject: [Histonet] RE: Ventana Labels We tried another vendor for ebar labels, FloPro Tek, easternlabsvc.com, but they didn't hold up in xylene. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Christopher Jacobs Sent: Thursday, December 26, 2013 12:39 PM To: 'histonet@lists.utsouthwestern.edu' Cc: Irene G. Campbell, HT (ASCP) Subject: [Histonet] Ventana Labels Histonetters, Like everywhere else, my lab is trying to find ways to save money. One thing we are looking at are the labels/ribbon we use for our Ventana EBAR printers and our Ventana Vantage Zebra printers. Currently, we order labels/printer ribbons for both types of printers directly from Ventana. As one can imagine, we are paying a premium price. Has any out there had any luck finding labels/ribbon cheaper from other sources? Thank you everyone! -CJ CONFIDENTIALITY NOTICE: This message and any attachments are for the sole use of the intended recipient(s). This message is confidential and may also be privileged. If you are not the intended recipient, please contact the sender immediately, delete the contents of this message and do not use it for any purpose. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpwenk <@t> sbcglobal.net Thu Dec 26 17:10:55 2013 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Thu Dec 26 17:11:05 2013 Subject: [Histonet] LABORATORY SAFETY TEST In-Reply-To: <04EE4F75BB5FB246ADB68D69B74604438E3B821F66@MAIL.nrhnt.nrh-ok.com> References: <04EE4F75BB5FB246ADB68D69B74604438E3B821F66@MAIL.nrhnt.nrh-ok.com> Message-ID: <080E94D4259240B9A12CB311312B92FB@HP2010> I took the exam when it was first offered, when it was a Specialist exam with 100 questions. It's now a Qualification exam with 50 questions. But I don't think it has changed that much, just fewer questions. If you haven't been to the ASCP website on the Safety Exam, do that first. http://ascp.org/Board-of-Certification/Qualification#tabs-0 Make certain you meet eligibility requirements, and can document it. Under the Studying tab, there is information on reading list and the topic outline. - In addition to the books listed to read, I would get the Dapson and Dapson book on Hazardous Material in the Histopathology Laboratory. I bought mine from Anatech Ltd (www.anatechltdusa.com), but I think it's available through Amazon, also. - I did go to many of the websites they now list (back then, they didn't list websites, so I looked them up). And then looked up the topics under CDC, OSHA, NFPA, etc. - I also looked up information under companies, such as chemical hoods and biological hoods - many of the companies that sell them have a lot of good information as to how they are made, types of filters, air flow patterns, and remember to look up both chemical and biological (and sub-categories). - NSH has an on-line self-assessment booklet on Safety - a little over 100 multiple choice questions that you can either study from or test yourself on. Good explanations after it gives you the correct answer. Self-Assessment #14. (Disclaimer - I was editor on the booklet. I wrote it after I had taken the SLS exam. I pulled the questions not from the exam, but from all my study notes I had pulled together. I don't get any money from the self-assessment - NSH gets it.) It's $35, you can retake the exam as many times as you want over a 2 year period. I think you can earn continuing education credits from NSH for taking the exam, but I don't remember how many CE's it is worth. http://www.softconference.com/nsh/NSHCourseList.asp I also read our hospital's policies written by our safety department, epidemiology, and clinical pathology. Realize that ASCP is writing safety questions for ALL labs, so it's important to know clinical pathology (CP) safety - microbiology including some of the common genus/species of common dangerous infections (yersinia pestis = plague), packaging/shipping of tubes of blood, blood safety in general. In fact, when I took it, more of the questions related to CP safety than to anatomic pathology (AP) safety. Maybe I just was in a pocket of CP questions. But afterwards, I did look up who was on the ASCP safety exam committee who wrote the questions, and I think there were 5 CP techs/pathologists, and only 1 AP histotech. (But remember, many questions are both AP and CP - fire, acid cabinets, first aid, etc.) Definitely know your organizations - a whole alphabet of agencies. Hope this helps. As Safety Officer of Anatomic Pathology, I was always worried that something might happen, and someone would get hurt. And that they might sue the hospital, the department and/or me. And I could see a lawyer asking me "And what makes YOU qualified to be a Safety Officer?" Any my answer of - "well, no one else wanted the job that doesn't pay you any extra, and I don't know how to say no" would not go over very well with a jury. So I figured having passed the ASCP exam would give me some credentialing as being qualified to be a safety officer. Peggy A. Wenk, HTL(ASCP)SLS -----Original Message----- From: Adesupo, Adesuyi (Banjo) Sent: Thursday, December 26, 2013 1:53 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] LABORATORY SAFETY TEST Hi, I am interested in doing the ASCP qualification in laboratory safety test and I will appreciate it, if you guys in histoland could assist/help me with some hints on how to prepare for this test. Thanking you all for your anticipated cooperation. Banjo Adesuyi, BSMT, HT (ASCP), HTL (ASCP), QIHC (ASCP) Histology Supervisor Norman Regional Health System, Norman, OK 73071. Tel: 405- 307- 1145 ====================================== CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From katelin09htl <@t> gmail.com Fri Dec 27 22:27:52 2013 From: katelin09htl <@t> gmail.com (Katelin Lester) Date: Fri Dec 27 22:27:57 2013 Subject: [Histonet] LABORATORY SAFETY TEST In-Reply-To: <04EE4F75BB5FB246ADB68D69B74604438E3B821F66@MAIL.nrhnt.nrh-ok.com> References: <04EE4F75BB5FB246ADB68D69B74604438E3B821F66@MAIL.nrhnt.nrh-ok.com> Message-ID: I just took (and passed!) the QLS exam this month. My test included several questions about needle stick injuries, chemical spills and safety, and fire safety. The list they have is a great start for studying but I would also get the books they recommend. I admit the test was more difficult than I expected. Good luck! Katelin, HTL On Dec 26, 2013 10:53 AM, "Adesupo, Adesuyi (Banjo)" wrote: > Hi, > I am interested in doing the ASCP qualification in laboratory safety > test and I will appreciate it, if you guys in histoland could assist/help > me with some hints on how to prepare for this test. > Thanking you all for your anticipated cooperation. > > > Banjo Adesuyi, BSMT, HT (ASCP), HTL (ASCP), QIHC (ASCP) > Histology Supervisor > Norman Regional Health System, > Norman, OK 73071. > Tel: 405- 307- 1145 > > > > ====================================== > CONFIDENTIALITY NOTICE: > > This e-mail communication and any attachments may > contain confidential and privileged information for the use > of the designated recipients named above. If you are not > the intended recipient, you are hereby notified that you > have received this communication in error and that any > review, disclosure, dissemination, distribution, or copying > of it or its contents is prohibited. If you have received > this communication in error, please notify the sender > immediately and destroy all copies of this communication > and any attachments. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From lindseymarkovic28 <@t> hotmail.com Sat Dec 28 06:45:37 2013 From: lindseymarkovic28 <@t> hotmail.com (Lindsey Markovic) Date: Sat Dec 28 06:45:46 2013 Subject: [Histonet] QIHC Message-ID: This message is for any histotechs who have taken the QIHC. I have read all of the posts about what to study and what is on it, but my question is how is the test structured? Is it similar to the HT/HTL certification exam where each question builds on the one before it and they try to "trick" you with multiple answers that could be right and you have to choose the best answer? Or are the questions pretty straightforward? Thank you for any help you can give me! From lpwenk <@t> sbcglobal.net Sat Dec 28 07:30:01 2013 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Sat Dec 28 07:30:05 2013 Subject: [Histonet] QIHC In-Reply-To: References: Message-ID: <9A73AD69250B473CADD861FF7FE89595@HP2010> All ASCP tests (HT/HTL, HT/MLT, CT, PB, QIHC, QLS, etc.) are all built the same. All are multiple choice questions, with 4 choices, one of which is the most correct. (I won't say only 1 is correct, but if 1 is always correct or almost always correct except in weird circumstances, and another one MIGHT be correct under a very strange, weird, rarely to happen circumstance, then you are to pick the one that is always (or almost always) correct. Each question has been rated on degree of difficulty from 100-999 points, with 400 points being passing. But the points don't really mean anything, ASCP could have rated the questions A through Z, with J+ or I minus minus being passing. So a 321 point question is slightly harder than a 319, but much easier than a 403. And 721 is really hard. The person starts out with questions on the easy to just barely passing side. If they get that one correct, they get a slightly harder question. If they get that correct, they get one even more slightly hard. When they get one wrong, they get a slightly easier question. Whatever the last question degree of difficulty is, that determines if pass or not - 400 and above = pass; 399 or below = fail. The topics of the questions are randomly given out, but always the test will end up within the percent of topics as indicated under the study guide (e.g., QIHC has 10% of the questions on general immuno, but 30% on immuno staining). There are three taxonomy levels of questions, also: - Tax I = memorize - Tax II = troubleshoot (what went wrong); lab math; interpreting charts or photos - Tax III = problem-solve (how to fix problem); could include some charts or photos So all the ASCP tests are more than just memorizing the reagents, or the colors, or the steps. It's being able to figure out if something (stain, results, fixation, whatever) is good or not, and if it isn't - why, and if it isn't - what can you do to fix it now, or what are you going to do different next time so it doesn't happened again. Good luck to anything taking any ASCP certification or qualification exam in 2014! Peggy A. Wenk, HTL(ASCP)SLS -----Original Message----- From: Lindsey Markovic Sent: Saturday, December 28, 2013 7:45 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] QIHC This message is for any histotechs who have taken the QIHC. I have read all of the posts about what to study and what is on it, but my question is how is the test structured? Is it similar to the HT/HTL certification exam where each question builds on the one before it and they try to "trick" you with multiple answers that could be right and you have to choose the best answer? Or are the questions pretty straightforward? Thank you for any help you can give me! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From c.tague <@t> Pathologyarts.com Mon Dec 30 09:33:07 2013 From: c.tague <@t> Pathologyarts.com (Curt) Date: Mon Dec 30 08:26:16 2013 Subject: [Histonet] anyone able to help with some PCP control tissue Message-ID: <9C8F910F72893643B3C3793C3D67132B013FAEE1@PATHOLOGYSERVER.pathologyarts.local> I'm about out and don't have any sources (hospitals) that either have any or are willing to release it. Will gladly offer anything I have in return if there's something you might be short on. Can also provide our fed-ex number for shipping. Thanks for any help offered. Curt From Joyce.Weems <@t> emoryhealthcare.org Mon Dec 30 11:53:19 2013 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Mon Dec 30 11:53:27 2013 Subject: [Histonet] Annexin A1 Message-ID: Does anyone do this IHC stain? Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From abadesuyi <@t> nrh-ok.com Mon Dec 30 12:36:30 2013 From: abadesuyi <@t> nrh-ok.com (Adesupo, Adesuyi (Banjo)) Date: Mon Dec 30 12:36:34 2013 Subject: [Histonet] P 16 Control Slides Message-ID: <04EE4F75BB5FB246ADB68D69B74604438E3B821F71@MAIL.nrhnt.nrh-ok.com> Hi, Please we are having trouble getting the P 16 Positive Control Slides. I will appreciate it, if you guys could tell me where to find one. Thanks, Banjo Adesuyi, BSMT, HT (ASCP), HTL (ASCP), QIHC (ASCP) Histology Supervisor Norman Regional Health System, Norman, OK 73071. Tel: 405- 307- 1145 ====================================== CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. From Joyce.Weems <@t> emoryhealthcare.org Mon Dec 30 12:50:49 2013 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Mon Dec 30 12:50:56 2013 Subject: [Histonet] RE: Annexin A1 In-Reply-To: References: Message-ID: Have an answer, folks.. thanks! Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Monday, December 30, 2013 12:53 PM To: Histonet Subject: [Histonet] Annexin A1 Does anyone do this IHC stain? Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Joyce.Weems <@t> emoryhealthcare.org Mon Dec 30 12:53:12 2013 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Mon Dec 30 12:53:20 2013 Subject: [Histonet] RE: Annexin A1 In-Reply-To: References: Message-ID: Have an answer, folks.. thanks! Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Monday, December 30, 2013 12:53 PM To: Histonet Subject: [Histonet] Annexin A1 Does anyone do this IHC stain? Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From workshopihc <@t> gmail.com Mon Dec 30 13:48:29 2013 From: workshopihc <@t> gmail.com (Workshop IHC) Date: Mon Dec 30 13:48:32 2013 Subject: [Histonet] IHC wet workshop anouncement Message-ID: Learn IHC staining, Hands - on IHC Lab course....CEU approved; February 27th & 28th , 2014 San Francisco Bay area. For more info contact Maria workshopihc@gmail.com From mucram11 <@t> comcast.net Mon Dec 30 14:12:53 2013 From: mucram11 <@t> comcast.net (Pam Marcum) Date: Mon Dec 30 14:13:19 2013 Subject: [Histonet] Annoucing: Arkansas Society for Histotechnology Spring Meeting In-Reply-To: <1194810847.57959.1388433801838.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Message-ID: <1222315036.58106.1388434373574.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> The Arkansas Society for Histotechnology would like to invite anyone interested to join us for a two meeting starting February 28th to March 1st, 2014 in Hot Springs AR.? This will be ASH's 40th Anniversary so we are planning a good time for all to go with the educational options.? ? We are in the process of completing the program which will have tips for basic IHC to more advanced automated systems as part of the offering.? Starting next year we will be required to have certain sign offs on safety with formaldehyde and the new SDS sheets (replacing MSDS sheets) and many other offerings.? Tissue Identification will be one of the other classes.? We publish the completed program as soon as possible.? We have a special rate with the hotel so please let us know if you are coming so we can get you the correct pricing and information. Shane Jones will offering a full day course for HT Readiness on Saturday for anyone preparing to take the examination this spring.? If you would like further information or to join us please send me an e-mail at ashnews13@yahoo.com and I will forward it ASAP.? Thank You, Pam Marcum Vendor Coordinator/Newsletter Editor From ggracie <@t> stvincents.com.au Mon Dec 30 16:48:46 2013 From: ggracie <@t> stvincents.com.au (Gary Gracie) Date: Mon Dec 30 16:49:01 2013 Subject: [Histonet] Annexin A1 Message-ID: <1ec976f042f7492b90d0b507b796fae6@SVMHSEXCH02.svmhs.stvincents.com.au> Dear Joyce, We do Annexin A1. What would you like to know? Gary Gracie Senior Technical Officer IHC Laboratory Anatomical Pathology St Vincents Hospital Sydney Australia Message: 2 Date: Mon, 30 Dec 2013 17:53:19 +0000 From: "Weems, Joyce K." Subject: [Histonet] Annexin A1 To: Histonet Message-ID: Content-Type: text/plain; charset="us-ascii" Does anyone do this IHC stain? Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the system manager. This footnote also confirms that this email message has been virus scanned and although no viruses were detected by the system, St Vincents & Mater Health Sydney accepts no liability for any consequential damage resulting from email containing any computer viruses. ********************************************************************** From tahseen <@t> brain.net.pk Mon Dec 30 23:35:07 2013 From: tahseen <@t> brain.net.pk (tahseen) Date: Mon Dec 30 23:35:16 2013 Subject: [Histonet] P 16 Control Slides In-Reply-To: <04EE4F75BB5FB246ADB68D69B74604438E3B821F71@MAIL.nrhnt.nrh-ok.com> References: <04EE4F75BB5FB246ADB68D69B74604438E3B821F71@MAIL.nrhnt.nrh-ok.com> Message-ID: <7463e7b44b9e9b1086ad9e114fdcb50e@brain.net.pk> Dear Banjo, We perform moderately differentiated squamous cell carcinoma Cervix as positive control for CINec p16 ventana with very good results. Regards Muhammad Tahseen n 2013-12-30 23:36, Adesupo, Adesuyi (Banjo) wrote: > Hi, > Please we are having trouble getting the P 16 Positive Control > Slides. I will appreciate it, if you guys could tell me where to find > one. > > Thanks, > > Banjo Adesuyi, BSMT, HT (ASCP), HTL (ASCP), QIHC (ASCP) > Histology Supervisor > Norman Regional Health System, > Norman, OK 73071. > Tel: 405- 307- 1145 > > > > ====================================== > CONFIDENTIALITY NOTICE: > > This e-mail communication and any attachments may > contain confidential and privileged information for the use > of the designated recipients named above. If you are not > the intended recipient, you are hereby notified that you > have received this communication in error and that any > review, disclosure, dissemination, distribution, or copying > of it or its contents is prohibited. If you have received > this communication in error, please notify the sender > immediately and destroy all copies of this communication > and any attachments. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Shannon.Burrage <@t> unitypoint.org Tue Dec 31 08:30:41 2013 From: Shannon.Burrage <@t> unitypoint.org (Burrage, Shannon L.) Date: Tue Dec 31 08:30:46 2013 Subject: [Histonet] FW: In-Reply-To: <8E6784497F13AC44A38383BCB11826AD55A228A2@TDCEXCHXM007.ihs.org> References: <8E6784497F13AC44A38383BCB11826AD55A228A2@TDCEXCHXM007.ihs.org> Message-ID: <8E6784497F13AC44A38383BCB11826AD55A228D6@TDCEXCHXM007.ihs.org> ________________________________ From: Burrage, Shannon L. Sent: Tuesday, December 31, 2013 8:10 AM To: ,utsouthwestern.edu Subject: Hi: I'm new to this website, and anxious to learn from all. At this point, i'm wondering if anyone could give me any information on the general use of bar coding in histology. Such as available vendors, and necessary equipment, mostly your personal experiences with this system. Thanks, Shannon shannon.burrage@unitypoint.org This message and accompanying documents are covered by the Electronic Communications Privacy Act, 18 U.S.C. ?? 2510-2521, and contain information intended for the specified individual(s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying, or the taking of any action based on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From TMcNemar <@t> lmhealth.org Tue Dec 31 09:14:52 2013 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Tue Dec 31 09:15:02 2013 Subject: [Histonet] FW: In-Reply-To: <8E6784497F13AC44A38383BCB11826AD55A228D6@TDCEXCHXM007.ihs.org> References: <8E6784497F13AC44A38383BCB11826AD55A228A2@TDCEXCHXM007.ihs.org> <8E6784497F13AC44A38383BCB11826AD55A228D6@TDCEXCHXM007.ihs.org> Message-ID: I am very happy with ours. Our system is from General Data. Specimen container labels are printed at accessioning, that label is scanned at grossing and cassettes are printed. Those cassettes are then scanned at the microtome and slide labels for that block only are printed from a small printer located at the microtome. We were unable to interface with our LIS (Meditech) so as a workaround, we have to print a report that sends the information over to the General Data system. There were some issues early on but it has proven to be a good system. I will be expanding it to include Cytology later this year. Tom McNemar, HT(ASCP) Histology Supervisor Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Burrage, Shannon L. Sent: Tuesday, December 31, 2013 9:31 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FW: ________________________________ From: Burrage, Shannon L. Sent: Tuesday, December 31, 2013 8:10 AM To: ,utsouthwestern.edu Subject: Hi: I'm new to this website, and anxious to learn from all. At this point, i'm wondering if anyone could give me any information on the general use of bar coding in histology. Such as available vendors, and necessary equipment, mostly your personal experiences with this system. Thanks, Shannon shannon.burrage@unitypoint.org This message and accompanying documents are covered by the Electronic Communications Privacy Act, 18 U.S.C. ?? 2510-2521, and contain information intended for the specified individual(s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying, or the taking of any action based on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From twheelock <@t> mclean.harvard.edu Tue Dec 31 10:41:09 2013 From: twheelock <@t> mclean.harvard.edu (Tim Wheelock) Date: Tue Dec 31 10:41:16 2013 Subject: [Histonet] Embedding paraffin for brain tissue? Message-ID: <52C2F3A5.9020102@mclean.harvard.edu> Hi Everyone: In November, there was a discussion concerning different types of embedding paraffin. Do people find that a certain kind of wax is preferable for embedding brain tissue, or doesn't it matter? I also use Surgipath EM-400, but have decided to try another brand. I have been using this paraffin for about 25 years. It used to cut like butter, with beautiful ribbons and no lines. I would put the blocks on ice, wait 2 hours, and then cut 7-8 cases (about 190 slides) per day easily. Now I am struggling to cut 4-5 cases. I am experiencing a lot of lines in my sections and some rolling and tearing of parts of the sections. This is affecting my lab's productivity, the quality of my sections, and is a source of constant frustration. I am cutting with Surgipath high profile Teflon coated blades. When I tried Thermo-Fisher HP-35 Ultra blades, at first they helped, but soon I experienced rolling of the sections. I have tried changing the angle of the blade, to no avail. So, I thought that I would try a change in the embedding paraffin. Thanks for any suggestions that you may have, Tim Wheelock Harvard Brain Bank McLean Hospital Belmont, MA From MICHELLE.LAMPHERE <@t> childrens.com Tue Dec 31 19:49:53 2013 From: MICHELLE.LAMPHERE <@t> childrens.com (Michelle Lamphere) Date: Tue Dec 31 19:49:59 2013 Subject: [Histonet] Histology program/school? Message-ID: Are there any histology programs or school in the Atlanta, GA area? If not, what would be a good place to start (in Atlanta) for somebody who wants to become a histotech? Michelle Lamphere, HT(ASCP) Lead Tech, Histology Department of Anatomic Pathology 1935 Medical District Dr. Dallas, TX 75235 214.456.2318 Please consider the environment before printing this e-mail This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. Please consider the environment before printing this e-mail This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information.