[Histonet] Positive tissue controls for MSI antibody panel

[joelle weaver]

normal colon, CRC, colon AC, another primary  




Joelle Weaver MAOM, HTL (ASCP) QIHC
 
> From: LSebree <@t> uwhealth.org
> To: Histonet <@t> lists.utsouthwestern.edu
> Date: Mon, 19 Aug 2013 17:55:40 +0000
> CC: 
> Subject: [Histonet] Positive tissue controls for MSI antibody panel
> 
> Question everyone:
> 
> What are you all using for your positive tissue control when running the MSI antibody panels, i.e. MLH-1, MSH-2, MSH-6, PMS-2?  We're contemplating using normal colon that all four antibodies should stain but do we also need to show that colon having the mutation does not stain for each antibody as well to show there are no false positive results?
> 
> The former would be the easy way out but I'm willing to construct a multi-tissue block containing normal colon and colon with the mutation(s).
> 
> Thanks for all your responses,
> 
> Linda A. Sebree
> University of Wisconsin Hospital & Clinics
> IHC/ISH Laboratory
> 600 Highland Ave.
> Madison, WI 53792
> (608)265-6596
> FAX: (608)262-7174
> 
> 
> 
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