[Histonet] Portable fans in the lab

joelle weaver joelleweaver <@t> hotmail.com
Mon Aug 5 06:54:51 CDT 2013


No arguments to anyone's tips about water on/within/underneath cut sections. I just use the fan though to help dry the hand coverslipped slides since I don't have a coverslipper.  In a hospital setting, they usually have to do the electrical check and provide the biomed sticker & certification. I have also seen hair dryers used. 




Joelle Weaver MAOM, HTL (ASCP) QIHC
 
> Date: Sun, 4 Aug 2013 12:31:40 -0400
> From: bakevictoria <@t> gmail.com
> To: rjbuesa <@t> yahoo.com
> Subject: Re: [Histonet] Portable fans in the lab
> CC: histonet <@t> lists.utsouthwestern.edu; paintedsplashes <@t> yahoo.com
> 
> Hi
> Rene is correct about standing them up right to drain, but if you are using
> charged slides water gets trapped underneath the sections because of the +
> charge of the slide itself.  If you see a bubble on the last section or
> around  sections on a ribbon you can release the water by just pin pricking
> them on the edge with a needle point or the tip of a fine
> tipped forceps. Tap the section and the water will drain away from the
> tissue.
> Joelle is on target with use of a fan if your lab has an increased or high
> humidity level as this will also affect the water retention on a slide.
> 
> 
> 
> 
> 
> 
> On Sun, Aug 4, 2013 at 11:50 AM, Rene J Buesa <rjbuesa <@t> yahoo.com> wrote:
> 
> > In my experience the best procedure is to place the slides with the
> > sections just "fished" from the water bath in a vertical position on the
> > short edge (1 plg).
> > By doing so the water that may be under the section will come out and
> > after about 5 minutes the slides can be placed in the rack and either go to
> > the oven to dry before manual staining or directly to the autostainer
> > heating station.
> > If you blow air over a section that is in horizontal position any water
> > trapped under the section will remain there and can cause the artifact
> > known as "nuclear bubbling".
> > René J.
> >
> >
> > ________________________________
> > From: Jeanne Clark <paintedsplashes <@t> yahoo.com>
> > To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu
> > >
> > Sent: Saturday, August 3, 2013 5:34 PM
> > Subject: [Histonet] Portable fans in the lab
> >
> >
> > Anyone using portable fans in Histology to facilitae drying of parafin
> > sections on slides prior to drying in oven?
> >
> > Jeanne
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