[Histonet] RE: Histonet Digest, Vol 113, Issue 30
Barr,Kaye H
khbarr <@t> mdanderson.org
Tue Apr 30 12:48:38 CDT 2013
What title do most use for someone who does gross and is not a PA?
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Tuesday, April 30, 2013 12:02 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 113, Issue 30
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Today's Topics:
1. BIG PROBLEM (Behnaz Sohrab)
2. Re: BIG PROBLEM (Linda)
3. Yearly Training (Nancy Schmitt)
4. HIER pressure cookers.... (k. leigh adams)
5. RE: HIER pressure cookers.... (Connolly, Brett M)
6. RE: HIER pressure cookers.... (Blazek, Linda)
7. Re: HIER pressure cookers.... (Jan Shivers)
8. RE: 5 gal specimen container search (Hannen, Valerie)
9. RE: 5 gal specimen container search (Shirley A. Powell)
10. RE: HIER pressure cookers.... (Dingersoll <@t> aplaboratories.com)
11. AW: [Histonet] BIG PROBLEM (Gudrun Lang)
12. Processor question - alcoholic formalin vs. buffered formalin
(Lake, Kim S)
13. RE: Processor question - alcoholic formalin vs. buffered
formalin (Hannen, Valerie)
14. cassette labelers (Margiotta-Watz, Michele)
----------------------------------------------------------------------
Message: 1
Date: Mon, 29 Apr 2013 10:36:01 -0700
From: "Behnaz Sohrab" <SohrabB1 <@t> ah.org>
Subject: [Histonet] BIG PROBLEM
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <517E4D11.4347.0054.1 <@t> ah.org>
Content-Type: text/plain; charset="us-ascii"
Good Morning, We have two Leica H&E stainer( old xt-5010 and new 5020). We do use : Gill's 3 for 6 minutes,
4% Glacial ACETIC Acid 40 seconds
Bluing : 45 sec. We done this for years!! last few months our Pantologists are complaining about GI Biopsies that they have TOO much blue in cytoplasma and the Mucin is too purple and blue? Please any suggestion??
Mean while we are trying the NEW kit from Leica for H&E , they think that is TOO pale?any of you has tried this new stain from Lecia?
With much application.
Behnaz Sohrab, Los Angeles
------------------------------
Message: 2
Date: Mon, 29 Apr 2013 11:30:38 -0700 (PDT)
From: Linda <lmdee1 <@t> yahoo.com>
Subject: Re: [Histonet] BIG PROBLEM
To: Behnaz Sohrab <SohrabB1 <@t> ah.org>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<1367260238.82176.YahooMailNeo <@t> web164002.mail.gq1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Hi,
?
I would first check with your alcohol and xylene suppliers to see if they changed anything.? When something?goes south it is usually because the manufacturer have changed.? This happened with my silver stains.
?
You may have to go back to the basics- harris hematoxylin, acid alcohol, and eosin y.? Richard Allen 7211 hematoxylin and the rest of their staining is excellent in these situations until you can get it back under control.? If you send them slides they will work a time out for you.
?
If you are using Leica's selectech, which is what I started this lab out with because I was heavily sampled out,?it has blue nuclei instead of the traditional purple.? I do 5 minutes in hematoxylin, 30 dips in clarifier and 1 minute in blue, 55 sec in eosin but I have (2) 95s after.? They also have a tech dept to help.? I have a Leica autostainer too.
?
I hope this helps.
?
Linda Dee, HT(ASCP)
?
________________________________
From: Behnaz Sohrab <SohrabB1 <@t> ah.org>
To: histonet <@t> lists.utsouthwestern.edu
Sent: Monday, April 29, 2013 12:36 PM
Subject: [Histonet] BIG PROBLEM
Good Morning, We have two Leica H&E stainer( old xt-5010 and new 5020). We do use : Gill's 3 for 6 minutes,
4% Glacial ACETIC Acid 40 seconds
Bluing : 45 sec. We done this for years!! last few months our Pantologists are complaining about GI Biopsies that they have TOO much blue in cytoplasma and the Mucin is too purple and blue? Please any suggestion??
Mean while we are trying the NEW kit from Leica for H&E , they think that is TOO pale?any of you has tried this new stain from Lecia?
With much application.
Behnaz Sohrab, Los Angeles
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------------------------------
Message: 3
Date: Mon, 29 Apr 2013 18:39:17 +0000
From: Nancy Schmitt <Nancy_Schmitt <@t> pa-ucl.com>
Subject: [Histonet] Yearly Training
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<906B4DA90ED1DB4DB6C7E94D7CEE6C36813C038E <@t> PEITHA.wad.pa-ucl.com>
Content-Type: text/plain; charset="us-ascii"
Cindy-
We also have a yearly competency packet. It follows JCAHO standards and includes direct observations, log documentation for QC and maintenance and problem solving through written exam.
Systems covered are H&E, IHC, Special stains, processing/embedding, Cytology prep, and Misc. Histology.
Nancy Schmitt
Histology Coordinator
Dubuque, IA
-----------------------------------------------------
From: Cindy Pyse [mailto:cpyse <@t> x-celllab.com]
Sent: Friday, April 26, 2013 4:49 PM
To: 'histonet-bounces <@t> lists.utsouthwestern.edu'
Subject: Yearly training
Happy Friday Everyone
I just wanted an opinion on what everyone's facilities train for annually.
An example is HIPPA training. I know what my regs require, I just wanted to see what everyone else in histoland was doing. Thanks in advance for all responses. I hope everyone has a great weekend. Finally we are going to have a good weather weekend in Western NY.
Cindy
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Message: 4
Date: Tue, 30 Apr 2013 09:29:33 -0400
From: "k. leigh adams" <k.leigh.adams.865 <@t> gmail.com>
Subject: [Histonet] HIER pressure cookers....
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<CAHot8dV4sR0aDAgb9+Ggbd6tH3CbrRbs0TdrB1BU0hobrHbYNQ <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Any input as to preferred instruments would be greatly appreciated...
Leigh
------------------------------
Message: 5
Date: Tue, 30 Apr 2013 09:53:50 -0400
From: "Connolly, Brett M" <brett_connolly <@t> merck.com>
Subject: RE: [Histonet] HIER pressure cookers....
To: "k. leigh adams" <k.leigh.adams.865 <@t> gmail.com>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<C01C35B84DCDCE49BC60867E87F1C8FE68D4403906 <@t> USCTMXP51015.merck.com>
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Biocare Decloaker here !!
Brett M. Connolly, Ph.D.
Principal Scientist, Imaging Dept.
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_connolly <@t> merck.com
T- 215-652-2501
F- 215-993-6803
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of k. leigh adams
Sent: Tuesday, April 30, 2013 9:30 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] HIER pressure cookers....
Any input as to preferred instruments would be greatly appreciated...
Leigh
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Message: 6
Date: Tue, 30 Apr 2013 09:58:58 -0400
From: "Blazek, Linda" <lblazek <@t> digestivespecialists.com>
Subject: RE: [Histonet] HIER pressure cookers....
To: "Connolly, Brett M" <brett_connolly <@t> merck.com>, "k. leigh adams"
<k.leigh.adams.865 <@t> gmail.com>, "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<5A2BD13465E061429D6455C8D6B40E391652B8729D <@t> IBMB7Exchange.digestivespecialists.com>
Content-Type: text/plain; charset="us-ascii"
I second that!
Linda Blazek HT (ASCP)
Manager/Supervisor
GI Pathology of Dayton
Digestive Specialists, Inc
Phone: (937) 396-2623
Email: lblazek <@t> digestivespecialists.com
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Connolly, Brett M
Sent: Tuesday, April 30, 2013 9:54 AM
To: k. leigh adams; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] HIER pressure cookers....
Biocare Decloaker here !!
Brett M. Connolly, Ph.D.
Principal Scientist, Imaging Dept.
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_connolly <@t> merck.com
T- 215-652-2501
F- 215-993-6803
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of k. leigh adams
Sent: Tuesday, April 30, 2013 9:30 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] HIER pressure cookers....
Any input as to preferred instruments would be greatly appreciated...
Leigh
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------------------------------
Message: 7
Date: Tue, 30 Apr 2013 09:34:56 -0500
From: Jan Shivers <shive003 <@t> umn.edu>
Subject: Re: [Histonet] HIER pressure cookers....
To: "Blazek, Linda" <lblazek <@t> digestivespecialists.com>
Cc: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>, "k. leigh adams"
<k.leigh.adams.865 <@t> gmail.com>
Message-ID:
<CAEoC1q3tgnQTBbQYuLKzwh2symq+MbYLNf_0PgD18SUo+4+mWg <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Third that.
Jan S
On Tue, Apr 30, 2013 at 8:58 AM, Blazek, Linda <
lblazek <@t> digestivespecialists.com> wrote:
> I second that!
>
> Linda Blazek HT (ASCP)
> Manager/Supervisor
> GI Pathology of Dayton
> Digestive Specialists, Inc
> Phone: (937) 396-2623
> Email: lblazek <@t> digestivespecialists.com
>
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:
> histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Connolly, Brett M
> Sent: Tuesday, April 30, 2013 9:54 AM
> To: k. leigh adams; histonet <@t> lists.utsouthwestern.edu
> Subject: RE: [Histonet] HIER pressure cookers....
>
> Biocare Decloaker here !!
>
> Brett M. Connolly, Ph.D.
> Principal Scientist, Imaging Dept.
> Merck & Co., Inc.
> PO Box 4, WP-44K
> West Point, PA 19486
> brett_connolly <@t> merck.com
> T- 215-652-2501
> F- 215-993-6803
>
>
>
>
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:
> histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of k. leigh adams
> Sent: Tuesday, April 30, 2013 9:30 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] HIER pressure cookers....
>
> Any input as to preferred instruments would be greatly appreciated...
>
> Leigh
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> Notice: This e-mail message, together with any attachments, contains
> information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New
> Jersey, USA 08889), and/or its affiliates Direct contact information for
> affiliates is available at
> http://www.merck.com/contact/contacts.html) that may be confidential,
> proprietary copyrighted and/or legally privileged. It is intended solely
> for the use of the individual or entity named on this message. If you are
> not the intended recipient, and have received this message in error, please
> notify us immediately by reply e-mail and then delete it from your system.
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
--
Jan Shivers
Senior Scientist
IHC/Histology Section Head
Pathology Teaching Program
UMN Veterinary Diagnostic Laboratory
University of Minnesota College of Veterinary Medicine
1333 Gortner Ave.
St. Paul, MN 55108
612-624-7297
shive003 <@t> umn.edu
------------------------------
Message: 8
Date: Tue, 30 Apr 2013 10:36:24 -0400
From: "Hannen, Valerie" <Valerie.Hannen <@t> parrishmed.com>
Subject: RE: [Histonet] 5 gal specimen container search
To: 'Jackie O'Connor' <b427297 <@t> aol.com>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <450B7A81EDA0C54E97C53D60F00776C3231B3D7C30 <@t> isexstore03>
Content-Type: text/plain; charset="us-ascii"
Jackie...
We have ordered these from a company by the name of Centurian Medical. The lids are sold separately.
Valerie
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jackie O'Connor
Sent: Wednesday, April 24, 2013 3:41 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] 5 gal specimen container search
Anyone in histoland know a vendor where I can find a 5 gallon leakproof bucket, drum, pail for shipping a large pathology specimen?
Jackie O'
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------------------------------
Message: 9
Date: Tue, 30 Apr 2013 10:38:59 -0400
From: "Shirley A. Powell" <POWELL_SA <@t> mercer.edu>
Subject: RE: [Histonet] 5 gal specimen container search
To: "Hannen, Valerie" <Valerie.Hannen <@t> parrishmed.com>, 'Jackie
O'Connor' <b427297 <@t> aol.com>, "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<9BF995BC0E47744E9673A41486E24EE2579B14884C <@t> MERCERMAIL.MercerU.local>
Content-Type: text/plain; charset="us-ascii"
You can also check with restaurant supply houses, we have purchased great large containers with lids for this purpose. Much cheaper than the lab supply houses.
Shirley
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie
Sent: Tuesday, April 30, 2013 10:36 AM
To: 'Jackie O'Connor'; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] 5 gal specimen container search
Jackie...
We have ordered these from a company by the name of Centurian Medical. The lids are sold separately.
Valerie
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jackie O'Connor
Sent: Wednesday, April 24, 2013 3:41 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] 5 gal specimen container search
Anyone in histoland know a vendor where I can find a 5 gallon leakproof bucket, drum, pail for shipping a large pathology specimen?
Jackie O'
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=============
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------------------------------
Message: 10
Date: Tue, 30 Apr 2013 07:40:51 -0700
From: <Dingersoll <@t> aplaboratories.com>
Subject: RE: [Histonet] HIER pressure cookers....
To: "Blazek, Linda" <lblazek <@t> digestivespecialists.com>, "Connolly,
Brett M" <brett_connolly <@t> merck.com>, "k. leigh adams"
<k.leigh.adams.865 <@t> gmail.com>, "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<20130430074051.073ecbdb5144cf8a05e574ee22bfb11a.114d0c0283.wbe <@t> email17.secureserver.net>
Content-Type: text/plain; charset="utf-8"
dingersoll <@t> aplaboratories.com
-------- Original Message --------
Subject: RE: [Histonet] HIER
From: "Blazek, Linda" <[1]lblazek <@t> digestivespecialists.com>
To: "Connolly, Brett M" <brett_connolly <@t> merck.com>, " adams"
<[2]k. "[3]histonet <@t> lists.utsouthwestern.edu"
<[4]histonet <@t> lists.utsouthwestern.edu<
I second that!
Linda Blazek HT (ASCP)
GI Pathology of Dayton
Digestive Speciali
Phone: (937) 396-2623
Email: [5]lblazek <@t> digestivespecialists.com
-----Original Message-----
From: [6]histonet-bounces <@t> lists.utsouthwes mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Connolly
Sent: Tuesday, April 30, 2013 9:54 AM
To: k. leigh ad
Subject: RE: [Histonet] HIER pressure cookers...
Biocare Decloaker here !!
Brett M. Connolly, Ph
Principal Scientist, Imaging Dept.
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
[9]brett_connolly <@t> merck.com
T- 215-652-2501
F- 215-993-6803
-----O
From: [10]histonet-bounces <@t> lists.utsouthwestern.edu
[[11]mailto:histonet-bounc k. leigh adams
Sent: Tu
To: [12]histonet <@t> lists.utsouthwestern.edu
Subject: [His
Any input as to preferred ins appreciated...
Leigh
_________
Histonet mailing list
[13]Histonet <@t> lists.utsouthwe
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Notice: This e-mail message, together with any attachments, contai ns information of Merck & Co., Inc. (One Merck Drive, Whitehouse
Statio contact information
[15]http://www.merck.com/contact/contacts.html) that may confidential, proprietary copyrighted and/or legally privileged. It is
i this messag received this message in e-mail and then delete it fro
________________________________________
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[16]Histonet <@t> lists.utsouthwestern.edu
[17]http://lists.uts
________________
Histonet mailing list
[18]Histonet <@t> lists.utsouthwestern
[19]http://lists.utsouthwestern.edu/mailman/listinfo/histonet
References
1. 3D"mailto:lbla 2. 3D"mailto:k.leigh.adams.865 <@t> gmail.com"
3. 3D"mailto:histonet <@t> lists.utsout 4. file://localhost/tmp/3D"m 5. 3D"mailto:lblazek 6. 3D"mailto:h 7. 3D"mailto:histonet-bounces <@t> lists.utsouthwestern.edu"
8. 3D"mailto:histonet <@t> lists.utsouthwestern.edu"
9. 3D"mailto:bre 10. 3D"mailto:histonet-bounces <@t> lists.u 11. ="mailto:histonet-bounces <@t> lists.utsouthwestern.edu"
12. 3D"mailto:histonet <@t> lists.ut 13. 3D"mailto:Histonet <@t> lists.utsouthwestern.edu"
14. 3D"http://lists.utsouthwestern.edu/mailman/list 15. 3D"http://www.merck.com/conta 16. 3D"mailto:Histonet <@t> lists. 17. file://localhost/tmp/3D" 18. ="mailto:Histonet <@t> lists.utsouthwestern.edu"
19. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/
------------------------------
Message: 11
Date: Tue, 30 Apr 2013 17:15:01 +0200
From: "Gudrun Lang" <gu.lang <@t> gmx.at>
Subject: AW: [Histonet] BIG PROBLEM
To: "'Behnaz Sohrab'" <SohrabB1 <@t> ah.org>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <000301ce45b5$7d292a50$777b7ef0$@gmx.at>
Content-Type: text/plain; charset="iso-8859-1"
Too much blue is mostly a problem of too less red.
Have you checked your Eosin? pH? Rinsing after eosin?
My personal issue was sometimes too thin sections. Those looked also rather
pale.
Gudrun
-----Urspr?ngliche Nachricht-----
Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Behnaz
Sohrab
Gesendet: Montag, 29. April 2013 19:36
An: histonet <@t> lists.utsouthwestern.edu
Betreff: [Histonet] BIG PROBLEM
Good Morning, We have two Leica H&E stainer( old xt-5010 and new 5020). We
do use : Gill's 3 for 6 minutes, 4% Glacial ACETIC Acid 40 seconds Bluing :
45 sec. We done this for years!! last few months our Pantologists are
complaining about GI Biopsies that they have TOO much blue in cytoplasma and
the Mucin is too purple and blue? Please any suggestion??
Mean while we are trying the NEW kit from Leica for H&E , they think that is
TOO pale?any of you has tried this new stain from Lecia?
With much application.
Behnaz Sohrab, Los Angeles
------------------------------
Message: 12
Date: Tue, 30 Apr 2013 15:30:33 +0000
From: "Lake, Kim S" <kim-lake <@t> uiowa.edu>
Subject: [Histonet] Processor question - alcoholic formalin vs.
buffered formalin
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<F3181E1428AD274EAEF67664C5B662AB25827126 <@t> itsnt443.iowa.uiowa.edu>
Content-Type: text/plain; charset="us-ascii"
I attended the Tri-State Symposium in Dubuque, IA last week (which was fantastic) and sat in on a "Boot Camp for Histology" workshop. At this workshop I realized that some problems we have been having with sectioning could be due to our processor set up.
Before microtomy we have to soak our blocks for 10-15 minutes, and I suspect that this is because we are over dehydrating our tissues in the during processing. Our first solution, which the cassettes sit in for about 8 hours until the overnight processing begins, is alcoholic formalin, which is 450ml buffered formalin, 900ml water, and 3300ml 95% alcohol. Our processor is a Leica TP 1050 that has been chugging along for about 20 years now.
The reason we use alcoholic formalin instead of just normal buffered formalin has been lost to the mists of time, and I was wondering what you all use as a holding solution on your processor, and why.
Thanks!
Kim Lake
Laboratory Manager
Oral Pathology Laboratory
University of Iowa College of Dentistry
Phone (319) 384 4433
Fax (319) 353 5569
------------------------------
Message: 13
Date: Tue, 30 Apr 2013 11:40:53 -0400
From: "Hannen, Valerie" <Valerie.Hannen <@t> parrishmed.com>
Subject: RE: [Histonet] Processor question - alcoholic formalin vs.
buffered formalin
To: "'Lake, Kim S'" <kim-lake <@t> uiowa.edu>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <450B7A81EDA0C54E97C53D60F00776C3231B3D7C33 <@t> isexstore03>
Content-Type: text/plain; charset="us-ascii"
We use 10 % Neutral Buffered Formalin. It is a wonderful fixative and gives us really great fixation.
It does sound like you are overly dehydrating your tissues.
Valerie A. Hannen, MLT(ASCP),HTL,SU(FL)
Histology Section Chief
Parrish Medical Center
951 N. Washington Ave.
Titusville, Florida 32976
Phone:(321) 268-6333 ext. 7506
Fax: (321) 268-6149
valerie.hannen <@t> parrishmed.com
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Lake, Kim S
Sent: Tuesday, April 30, 2013 11:31 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Processor question - alcoholic formalin vs. buffered formalin
I attended the Tri-State Symposium in Dubuque, IA last week (which was fantastic) and sat in on a "Boot Camp for Histology" workshop. At this workshop I realized that some problems we have been having with sectioning could be due to our processor set up.
Before microtomy we have to soak our blocks for 10-15 minutes, and I suspect that this is because we are over dehydrating our tissues in the during processing. Our first solution, which the cassettes sit in for about 8 hours until the overnight processing begins, is alcoholic formalin, which is 450ml buffered formalin, 900ml water, and 3300ml 95% alcohol. Our processor is a Leica TP 1050 that has been chugging along for about 20 years now.
The reason we use alcoholic formalin instead of just normal buffered formalin has been lost to the mists of time, and I was wondering what you all use as a holding solution on your processor, and why.
Thanks!
Kim Lake
Laboratory Manager
Oral Pathology Laboratory
University of Iowa College of Dentistry
Phone (319) 384 4433
Fax (319) 353 5569
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Message: 14
Date: Tue, 30 Apr 2013 16:31:49 +0000
From: "Margiotta-Watz, Michele" <MMargiotta <@t> bmhmc.org>
Subject: [Histonet] cassette labelers
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<230D0B9EC57D7A45A7A186C6AB4C7ABC296F39AA <@t> BMH-EXCHANGE-02.BMHMC.ORG>
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Hi All,
We are looking to purchase 2 cassette labelers and would like some feedback on which ones you might recommend. Also, is there a difference between Thermo's Printmate and Microwriter? Looks like the Printmate is just a newer version. Your input would be greatly appreciated.
Thanks,
Michele
Histology Supervisor
BMHMC
631-654-7192
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End of Histonet Digest, Vol 113, Issue 30
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