[Histonet] BIG PROBLEM

[Behnaz Sohrab]

Good Morning, We have two Leica H&E stainer( old xt-5010 and new 5020). We do use : Gill's 3 for 6 minutes,
4% Glacial ACETIC Acid 40 seconds
Bluing : 45 sec. We done this for years!! last few months our Pantologists are complaining about GI Biopsies that they have TOO much blue in cytoplasma and the Mucin is too purple and blue? Please any suggestion??
Mean while we are trying the NEW kit from Leica for H&E , they think that is TOO pale?any of you has tried this new stain from Lecia?
With much application.
Behnaz Sohrab, Los Angeles