[Histonet] Paraplast Xtra Melting Point?

[Esther C Peters]

I have wondered about that myself recently. Noticed lower temperature needed in our waterbaths and melting at lower temperature in embedding center. Shiny drops form on top of our blocks, but not with the tissue.

Esthr

Esther C. Peters, Ph.D.
Assistant Professor
Environmental Science & Policy
Biology Program, Medical Technology Coordinator
George Mason University

________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] on behalf of Marsh, Nannette [NMP <@t> stowers.org]
Sent: Friday, April 26, 2013 11:29 AM
To: 'Rene J Buesa'; Jones, Laura; Histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Paraplast Xtra Melting Point?

On the back of the bag are the instructions : 52 degrees melting point:  for best results, use at temperatures above 56 degrees.  Would it make a difference if it melted at the lower temperature but, embedding is done at the warmer temperature?   Just wondering.  Thank you for your response.
 Nanne Marsh

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Friday, April 26, 2013 8:52 AM
To: Jones, Laura; Histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Paraplast Xtra Melting Point?

The "rule of thumb" is that the lower the paraffin melting point is the higher the compression rate is.
It would be nice to ask the manufacturer why the change; perhaps they have added something that reduces the melting point, but I really do not think they should do that without warning the users.
René J.

From: "Jones, Laura" <lpjones <@t> srhs-pa.org>
To: "Histonet <@t> lists.utsouthwestern.edu" <Histonet <@t> lists.utsouthwestern.edu>
Sent: Friday, April 26, 2013 9:41 AM
Subject: [Histonet] Paraplast Xtra Melting Point?


Happy Friday to all!  For those of you who use Paraplast Xtra, had you noticed that the melting point printed on the bag has changed from 56 degrees to 52 degrees?  I've seen several comments recently about problems with compression with this paraffin; and we have been experiencing the same problem.  A friend and former coworker contacted me about similar problems they are having in her new lab, and they had noticed the change in the melting point.  We get our Paraplast Xtra from Fisher, but it's difficult to say who is actually making it from the information on the bag.  Does anyone else have experiences to share?  I'm uncertain if this is the cause of our compression issues, but I'm throwing it out there for all of you experts.  Thank you in advance!

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