[Histonet] Blades
Lyn Stadler
LStadler <@t> cbiolabs.com
Wed Apr 24 09:16:14 CDT 2013
I generally use CL Sturkey Extremus Low Profile Blades for pretty much everything...when I am having trouble with intestine sections, I use the DuraEdge Encore blade and it usually works very well.
Lyn M. Stadler, BS, HTL(ASCP)CM
Research Histotechnologist
Department of Histopathology
Cleveland Biolabs, Inc.
73 High Street
Buffalo, NY 14203
716-849-6817, ext 417
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Tuesday, April 23, 2013 1:05 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 113, Issue 24
Send Histonet mailing list submissions to
histonet <@t> lists.utsouthwestern.edu
To subscribe or unsubscribe via the World Wide Web, visit
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
or, via email, send a message with subject or body 'help' to
histonet-request <@t> lists.utsouthwestern.edu
You can reach the person managing the list at
histonet-owner <@t> lists.utsouthwestern.edu
When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..."
Today's Topics:
1. RE: Happy Lab Week from Pam Barker and RELIA Solutions!!
(Ian R Bernard)
2. staining near pap pen only???? (Emily Sours)
3. Re: staining near pap pen only???? (Emily Sours)
4. RE: Direct Immunofluorescence on Benchmark XT/Ultra (Sue Hunter)
5. RE: Direct Immunofluorescence on Benchmark XT/Ultra
(Vanessa Perez)
6. RE: Direct Immunofluorescence on Benchmark XT/Ultra
(Vanessa Perez)
7. RE: Direct Immunofluorescence on Benchmark XT/Ultra
(Vanessa Perez)
8. Forwarded post for a histonet member: Subject: CLIA 24-hour
gross review. (Sandy Cope-yokoyama)
9. RE: Blades (McAnn, Sherrian)
10. New Lab (Hale, Meredith)
----------------------------------------------------------------------
Message: 1
Date: Mon, 22 Apr 2013 17:22:13 +0000
From: Ian R Bernard <ibernard <@t> uab.edu>
Subject: RE: [Histonet] Happy Lab Week from Pam Barker and RELIA
Solutions!!
To: Pam Barker <relia1 <@t> earthlink.net>, Histonet
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<D4F4C602B10B9F45B4E9271AF6380E16181B7E43 <@t> UABEXMB1.ad.uab.edu>
Content-Type: text/plain; charset="iso-8859-1"
- We are celebrating with sponsor breakfasts and lunches for lab personnel from local restaurants each day.
- Different internal games.
- Educational Lab Week information and mementos for patients and staff alike
- And finally, two half days this week, some personnel will go hiking and others on another day horseback riding. That way our mission is staffed without negative impact to patient care
V/r
Ian R. Bernard, MSHA, HT (ASCP)
NCOIC-Manager, Anatomic Pathology Lab
10th Medical Group
USAF Academy, CO 80840
Graduate Certificate In Gerontology Student-UAB
210-687-7540
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Pam Barker
Sent: Monday, April 22, 2013 11:49 AM
To: Histonet
Subject: [Histonet] Happy Lab Week from Pam Barker and RELIA Solutions!!
Hi Histonetters.
Happy Lab Week!!
Wow this year is just flying by!! It was just Histotechnology Professionals Day a short time ago and here it is lab week already!
So here is my question.
How is your facility celebrating Lab Week?
I hope you are gearing up for a fun week and if you need some ideas for lab week check out Advance Magazine, they have some fun ideas.
I think we both know it wouldn't be an email from me if I didn't tell you about my current openings.
Please take a second and check them out.
Here Are My Spotlight Opportunities:
Night Shift Histotech - Patterson, NJ Brand New Lab!!
Day Shift Histotech - Salem, VA beautiful area and great Team HT/HTL Mohs Histotech - Long Beach, CA - Make it your own brand new in office lab!
The rest of the histology positions that I am most excited about are located in these areas:
Nashville, TN
Waco, TX
Tyler, TX -2 sites one at a hospital and one at a private dermpath lab Atlanta, GA Charlotte, NC Staunton, VA Louisville, KY Staunton, VA
Remember if you refer someone to me and I place them now or in the future You will earn a 500 dollar referral fee!!
If you think you or someone you know might be interested in any of these opportunities or would like to talk about a job search in another area, please contact me.
I can be reached at 866-607-3542 or relia1 <@t> earthlink.net?Thanks-Pam
Thanks-Pam
Right Place, Right Time, Right Move with RELIA!
Thank You!
?Pam M. Barker
?
Pam Barker
President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting
5703 Red Bug Lake Road #330
Winter Springs, FL 32708-4969
Phone: (407)657-2027
Cell:???? (407)353-5070
FAX:???? (407)678-2788
E-mail: relia1 <@t> earthlink.net
www.facebook.com/PamBarkerRELIA
www.linkedin.com/in/reliasolutions
www.twitter.com/pamatrelia
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 2
Date: Mon, 22 Apr 2013 13:23:46 -0400
From: Emily Sours <talulahgosh <@t> gmail.com>
Subject: [Histonet] staining near pap pen only????
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<CAP=XX1x-xixxbVPHWoKc8HTe9-v=gmLSe+tAp7Obt8mGo=YvwQ <@t> mail.gmail.com>
Content-Type: text/plain; charset=UTF-8
Hello Histonetters!
We have been doing triple staining on frozen tissue for a while. Just recently it started to stain only near the pap pen (the sections have two lines of pap pen, one where they begin and one where they end). This makes no sense to me, as the staining isn't only on one end (as if the tray was
crooked) but both ends and NOT the middle. Can anyone think of what would cause this?
My boss came up with the idea that the solution is evaporating and therefore, it's more concentrated on the ends, but that seems a stretch.
Then again, it's better than what I came up with, which is nothing!
Emily
"By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward."
-Chuck Palahniuk, "Haunted"
------------------------------
Message: 3
Date: Mon, 22 Apr 2013 13:35:48 -0400
From: Emily Sours <talulahgosh <@t> gmail.com>
Subject: Re: [Histonet] staining near pap pen only????
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<CAP=XX1xbGqW4OD-EXdR=h1yc+XvM9j5H1=vx9AhbeLJcjzad0w <@t> mail.gmail.com>
Content-Type: text/plain; charset=UTF-8
Sorry, I forgot to mention, this is manual staining.
Also, the area between the pap pen appears to be covered evenly with solution.
"By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward."
-Chuck Palahniuk, "Haunted"
On Mon, Apr 22, 2013 at 1:32 PM, Morken, Timothy < Timothy.Morken <@t> ucsfmedctr.org> wrote:
> Emily, It could be that the reagent is pooling off the ends of the tissue.
> Have you observed the tissue during staining to see what is happening
> to the liquid on the slide? Is this manual or automated?
>
> I agree it is strange, because the usual artifact you see with the pap
> pen is that the pen liquid covers part of the tissue and THAT part
> does not stain.
>
> Tim Morken
> Supervisor, Electron Microscopy/Neuromuscular Special Studies
> Department of Pathology UC San Francisco Medical Center
>
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:
> histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Emily Sours
> Sent: Monday, April 22, 2013 10:24 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] staining near pap pen only????
>
> Hello Histonetters!
>
> We have been doing triple staining on frozen tissue for a while. Just
> recently it started to stain only near the pap pen (the sections have
> two lines of pap pen, one where they begin and one where they end).
> This makes no sense to me, as the staining isn't only on one end (as
> if the tray was
> crooked) but both ends and NOT the middle. Can anyone think of what
> would cause this?
> My boss came up with the idea that the solution is evaporating and
> therefore, it's more concentrated on the ends, but that seems a stretch.
> Then again, it's better than what I came up with, which is nothing!
>
> Emily
>
>
> "By bitching and bitching and bitching, they could exhaust the drama
> of their own horror stories. Grow bored. Only then could they accept a
> new story for their lives. Move forward."
>
> -Chuck Palahniuk, "Haunted"
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
------------------------------
Message: 4
Date: Mon, 22 Apr 2013 19:29:56 +0000
From: Sue Hunter <SHUNTER <@t> beaumont.edu>
Subject: RE: [Histonet] Direct Immunofluorescence on Benchmark
XT/Ultra
To: Mel John del Barrio <meljdelbarrio <@t> yahoo.com>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<B493B3E4EF638A41875845CEDF938B440156A19A <@t> EXMail04.ms.beaumont.edu>
Content-Type: text/plain; charset="iso-8859-1"
We run our DIF on our old Lab Vision stainer because it is easier and cheaper. The Ultra ready to use Abs are very expensive and there is no negative control. As far as we can find out, there is no prep kit that you can use with the fluorescence protocols to make your own. Ventana's solution is to put your negative slide in a coplin jar filled with buffer and then add that to your slide tray when you are done.
We have the same issue with our Bond Max stainer - you have to use a detection kit for everything on the Bond so Leica's solution is to sell you a kit really cheap where you only use one solution and throw the rest away. Very expensive.
Since neither one of these "solutions" is an acceptable one for us, we still do them the "old"way and are very happy. I would think that unless you are running a really large number of cases, even doing them by hand is preferable to running them on the Ultra.
Just my opinion.....
Sue
Sue Hunter, Supervisor
Advanced Diagnostics
Beaumont Health System
Royal Oak MI
248-898-5146
shunter <@t> beaumont.edu
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Mel John del Barrio
Sent: Monday, April 22, 2013 12:49 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Direct Immunofluorescence on Benchmark XT/Ultra
Hi? All
?
Anyone in the group utilises the Benchmark for DIF's? What sort of controls do you use to validate the assay ? What problems? you have encountered?
?
?
Thanks
?
MJ
?
?
Image by FlamingText.com
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 5
Date: Mon, 22 Apr 2013 14:40:39 -0500
From: Vanessa Perez <vperez <@t> pathreflab.com>
Subject: RE: [Histonet] Direct Immunofluorescence on Benchmark
XT/Ultra
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<C80B5EE351AAD74DADAB70FABCB78D193BFEFF7BC6 <@t> exchange.pasa.local>
Content-Type: text/plain; charset="iso-8859-1"
We use Benchmark here with no problems. We use tonsil controls to validate it here. For the neg. we just have an extra slide sit in reaction buffer while the other slides are on the machine.
We switched from manual b/c less cost and less tech time.
Vanessa
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sue Hunter
Sent: Monday, April 22, 2013 2:30 PM
To: Mel John del Barrio; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Direct Immunofluorescence on Benchmark XT/Ultra
We run our DIF on our old Lab Vision stainer because it is easier and cheaper. The Ultra ready to use Abs are very expensive and there is no negative control. As far as we can find out, there is no prep kit that you can use with the fluorescence protocols to make your own. Ventana's solution is to put your negative slide in a coplin jar filled with buffer and then add that to your slide tray when you are done.
We have the same issue with our Bond Max stainer - you have to use a detection kit for everything on the Bond so Leica's solution is to sell you a kit really cheap where you only use one solution and throw the rest away. Very expensive.
Since neither one of these "solutions" is an acceptable one for us, we still do them the "old"way and are very happy. I would think that unless you are running a really large number of cases, even doing them by hand is preferable to running them on the Ultra.
Just my opinion.....
Sue
Sue Hunter, Supervisor
Advanced Diagnostics
Beaumont Health System
Royal Oak MI
248-898-5146
shunter <@t> beaumont.edu
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Mel John del Barrio
Sent: Monday, April 22, 2013 12:49 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Direct Immunofluorescence on Benchmark XT/Ultra
Hi? All
?
Anyone in the group utilises the Benchmark for DIF's? What sort of controls do you use to validate the assay ? What problems? you have encountered?
?
?
Thanks
?
MJ
?
?
Image by FlamingText.com
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 6
Date: Mon, 22 Apr 2013 15:22:29 -0500
From: Vanessa Perez <vperez <@t> pathreflab.com>
Subject: RE: [Histonet] Direct Immunofluorescence on Benchmark
XT/Ultra
To: "elonergan <@t> metrocast.net" <elonergan <@t> metrocast.net>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<C80B5EE351AAD74DADAB70FABCB78D193BFEFF7BD9 <@t> exchange.pasa.local>
Content-Type: text/plain; charset="iso-8859-1"
Yes, well when we take the ones off of the XT we add the neg and do the rinsing steps all together.
We do two rinses of reaction buffer for 5 min then final rinse with DI water...then coverslips....
Vanessa
-----Original Message-----
From: elonergan <@t> metrocast.net [mailto:elonergan <@t> metrocast.net]
Sent: Monday, April 22, 2013 3:10 PM
To: Vanessa Perez
Subject: Re: [Histonet] Direct Immunofluorescence on Benchmark XT/Ultra
Vanessa, we are just getting our DIF up and running. For your neg, it doesn't go thru any step, just coverslip direct from buffer? Thanks Eileen Lonergan MGH dermatopath associates.
Sent via BlackBerry by AT&T
-----Original Message-----
From: Vanessa Perez <vperez <@t> pathreflab.com>
Sender: histonet-bounces <@t> lists.utsouthwestern.edu
Date: Mon, 22 Apr 2013 14:40:39
To: histonet <@t> lists.utsouthwestern.edu<histonet <@t> lists.utsouthwestern.edu>
Subject: RE: [Histonet] Direct Immunofluorescence on Benchmark XT/Ultra
We use Benchmark here with no problems. We use tonsil controls to validate it here. For the neg. we just have an extra slide sit in reaction buffer while the other slides are on the machine.
We switched from manual b/c less cost and less tech time.
Vanessa
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sue Hunter
Sent: Monday, April 22, 2013 2:30 PM
To: Mel John del Barrio; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Direct Immunofluorescence on Benchmark XT/Ultra
We run our DIF on our old Lab Vision stainer because it is easier and cheaper. The Ultra ready to use Abs are very expensive and there is no negative control. As far as we can find out, there is no prep kit that you can use with the fluorescence protocols to make your own. Ventana's solution is to put your negative slide in a coplin jar filled with buffer and then add that to your slide tray when you are done.
We have the same issue with our Bond Max stainer - you have to use a detection kit for everything on the Bond so Leica's solution is to sell you a kit really cheap where you only use one solution and throw the rest away. Very expensive.
Since neither one of these "solutions" is an acceptable one for us, we still do them the "old"way and are very happy. I would think that unless you are running a really large number of cases, even doing them by hand is preferable to running them on the Ultra.
Just my opinion.....
Sue
Sue Hunter, Supervisor
Advanced Diagnostics
Beaumont Health System
Royal Oak MI
248-898-5146
shunter <@t> beaumont.edu
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Mel John del Barrio
Sent: Monday, April 22, 2013 12:49 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Direct Immunofluorescence on Benchmark XT/Ultra
Hi? All
?
Anyone in the group utilises the Benchmark for DIF's? What sort of controls do you use to validate the assay ? What problems? you have encountered?
?
?
Thanks
?
MJ
?
?
Image by FlamingText.com
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 7
Date: Tue, 23 Apr 2013 06:49:39 -0500
From: Vanessa Perez <vperez <@t> pathreflab.com>
Subject: RE: [Histonet] Direct Immunofluorescence on Benchmark
XT/Ultra
To: "Truscott, Tom" <ttruscot <@t> vetmed.wsu.edu>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<C80B5EE351AAD74DADAB70FABCB78D193BFEFF7BFE <@t> exchange.pasa.local>
Content-Type: text/plain; charset="iso-8859-1"
You just need the FITC antibodies. No kit required. The protocol is under the procedure Fluorescence IHC Basically all the machine does is dispense the antibody on the slide for however long you choose.
And some rinses of reaction buffer...
Vanessa
-----Original Message-----
From: Truscott, Tom [mailto:ttruscot <@t> vetmed.wsu.edu]
Sent: Monday, April 22, 2013 3:52 PM
To: Vanessa Perez
Subject: RE: [Histonet] Direct Immunofluorescence on Benchmark XT/Ultra
Hi Vanessa, I've been following this line of email, since I may need to start doing fluorescence on XT. Which kit/program/protocol do you use on the XT? Can you run them without a kit? Thankyou, Tom T
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Vanessa Perez
Sent: Monday, April 22, 2013 1:22 PM
To: elonergan <@t> metrocast.net; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Direct Immunofluorescence on Benchmark XT/Ultra
Yes, well when we take the ones off of the XT we add the neg and do the rinsing steps all together.
We do two rinses of reaction buffer for 5 min then final rinse with DI water...then coverslips....
Vanessa
-----Original Message-----
From: elonergan <@t> metrocast.net [mailto:elonergan <@t> metrocast.net]
Sent: Monday, April 22, 2013 3:10 PM
To: Vanessa Perez
Subject: Re: [Histonet] Direct Immunofluorescence on Benchmark XT/Ultra
Vanessa, we are just getting our DIF up and running. For your neg, it doesn't go thru any step, just coverslip direct from buffer? Thanks Eileen Lonergan MGH dermatopath associates.
Sent via BlackBerry by AT&T
-----Original Message-----
From: Vanessa Perez <vperez <@t> pathreflab.com>
Sender: histonet-bounces <@t> lists.utsouthwestern.edu
Date: Mon, 22 Apr 2013 14:40:39
To: histonet <@t> lists.utsouthwestern.edu<histonet <@t> lists.utsouthwestern.edu>
Subject: RE: [Histonet] Direct Immunofluorescence on Benchmark XT/Ultra
We use Benchmark here with no problems. We use tonsil controls to validate it here. For the neg. we just have an extra slide sit in reaction buffer while the other slides are on the machine.
We switched from manual b/c less cost and less tech time.
Vanessa
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sue Hunter
Sent: Monday, April 22, 2013 2:30 PM
To: Mel John del Barrio; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Direct Immunofluorescence on Benchmark XT/Ultra
We run our DIF on our old Lab Vision stainer because it is easier and cheaper. The Ultra ready to use Abs are very expensive and there is no negative control. As far as we can find out, there is no prep kit that you can use with the fluorescence protocols to make your own. Ventana's solution is to put your negative slide in a coplin jar filled with buffer and then add that to your slide tray when you are done.
We have the same issue with our Bond Max stainer - you have to use a detection kit for everything on the Bond so Leica's solution is to sell you a kit really cheap where you only use one solution and throw the rest away. Very expensive.
Since neither one of these "solutions" is an acceptable one for us, we still do them the "old"way and are very happy. I would think that unless you are running a really large number of cases, even doing them by hand is preferable to running them on the Ultra.
Just my opinion.....
Sue
Sue Hunter, Supervisor
Advanced Diagnostics
Beaumont Health System
Royal Oak MI
248-898-5146
shunter <@t> beaumont.edu
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Mel John del Barrio
Sent: Monday, April 22, 2013 12:49 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Direct Immunofluorescence on Benchmark XT/Ultra
Hi? All
?
Anyone in the group utilises the Benchmark for DIF's? What sort of controls do you use to validate the assay ? What problems? you have encountered?
?
?
Thanks
?
MJ
?
?
Image by FlamingText.com
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 8
Date: Tue, 23 Apr 2013 13:12:26 +0000
From: Sandy Cope-yokoyama <Sandy.Cope-Yokoyama <@t> childrens.com>
Subject: [Histonet] Forwarded post for a histonet member: Subject:
CLIA 24-hour gross review.
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<6F81318C8F5F984D972778B918A7D769310563 <@t> CMCPBEXMAIL12.Childrens.med>
Content-Type: text/plain; charset="us-ascii"
I am the sole histologist in a gastroenterology laboratory situated in a GI clinic. Complying with the 24-hour grossing review by the pathologist is a challenge because my slides travel by courier across town to the pathologist to read. I receive specimens throughout the day and perform grossing in the early AM then again at 11 AM. My pathologist signs copies of the requisition that I fax over that contains the gross and sends them back to me. On a daily basis this works just fine. Fridays and holidays are a pickle but we manage.
My question is the intent of the 24-hour review. The inspector cannot tell me why this was put in place, what the pathologist is supposed to do with this information and/or how to tell if it is accurate because he does not receive the slides until the following day which would be outside of the 24-hour review rule.
Can anyone tell the intent of the rule? How does this rule improve patient care? Even in a hospital laboratory, once a biopsy is grossed and placed in a cassette, how on earth does looking at the written gross of how big the specimen is or what color it is impact further actions that can take place? What remedial action does CLIA expect?
Thanks for your input, Gastro Gal
Please consider the environment before printing this e-mail
This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the
individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at privacy <@t> childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information.
Please consider the environment before printing this e-mail.
This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at privacy <@t> childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information.
------------------------------
Message: 9
Date: Tue, 23 Apr 2013 11:42:43 -0500
From: "McAnn, Sherrian" <Sherrian.McAnn <@t> va.gov>
Subject: RE: [Histonet] Blades
To: "Hale, Meredith" <mhale <@t> MiracaLS.com>,
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<61E2B58CECEF384094A363989D47C09009BA28B7 <@t> VHAV17MSGA2.v17.med.va.gov>
Content-Type: text/plain; charset="us-ascii"
I have been in histology and cutting for about 26 years now. I have used many types of blades, high and low profile. My favorite and I think the best ones are Surgipath Teflon coated high or low profile blades. I believe the high profile blades are the best but I do realize that not everyone has that option. I think if you tried them you wouldn't be disappointed.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Hale, Meredith
Sent: Monday, April 15, 2013 12:39 PM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Blades
I would like some feedback from you on the types of blades those of you who cut GI biopsies prefer ? Do you see differences with chatter on different blades. Any feedback is appreciated . Thanks !
Meredith Hale HT (ASCP)cm
Director External Sales Support
Miraca Life Sciences
6655 North MacArthur Blvd.
Irving , Texas 75039
Office: 214-596-2219
Cell: 469-648-8253
Fax: 1-866-688-3280
mhale <@t> miracals.com<mailto:mhale <@t> miracals.com<mailto:mhale <@t> miracals.com%3
cmailto:mhale <@t> miracals.com>>
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 10
Date: Tue, 23 Apr 2013 16:50:27 +0000
From: "Hale, Meredith" <mhale <@t> MiracaLS.com>
Subject: [Histonet] New Lab
To: "Histonet <@t> lists.utsouthwestern.edu"
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<0E828EC51C7CC445A51E53F81B64E8C722D557 <@t> s-irv-exchmb.PathologyPartners.intranet>
Content-Type: text/plain; charset="us-ascii"
Great opportunity for Histotechnician's in Crestview Hills, KY ! Tri-State Gastroenterology Associates is a multi-physician practice located in Northern Kentucky. Its mission is "To provide compassionate, high quality, cost-effective care to patients' with gastrointestinal problems" Looking for 2 Full Time HT/HTL's ( 32 hours a week is full time employment at this practice )
* Meet CLIA Grossing Requirements : CFR 493.1489, http://wwwn.cdc.gov/clia/regs/toc.aspx/ ,prior experience grossing GI specimens
* Supervisor experience preferred
* HT/HTL ASCP Certified
* Experience with CLIA and CAP
* Experience writing and maintaining policies and procedures
* Prior laboratory start up experience is preferred
* Ability to work independently
Duties include:
* Grossing
* Embedding
* Microtomy
* Staining; routine and special stains only
* Maintain supply orders and laboratory budget
* Ability to be flexible and take on additional duties' as needed
* Ability to work independently
* Maintenance of laboratory for inspections
* Maintenance of quality records
Interested applicants should contact Meredith Hale; phone 214-596-2219 or through email mhale <@t> miracals.com<mailto:mhale <@t> miracals.com>
Meredith Hale HT (ASCP)cm
Director External Sales Support
Miraca Life Sciences
6655 North MacArthur Blvd.
Irving , Texas 75039
Office: 214-596-2219
Cell: 469-648-8253
Fax: 1-866-688-3280
mhale <@t> miracals.com<mailto:mhale <@t> miracals.com<mailto:mhale <@t> miracals.com%3cmailto:mhale <@t> miracals.com>>
------------------------------
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
End of Histonet Digest, Vol 113, Issue 24
*****************************************
This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information.
More information about the Histonet
mailing list