[Histonet] staining near pap pen only????

[Emily Sours]

Sorry, I forgot to mention, this is manual staining.
Also, the area between the pap pen appears to be covered evenly with
solution.

"By bitching and bitching and bitching, they could exhaust the drama of
their own horror stories. Grow bored. Only then could they accept a new
story for their lives. Move forward."

-Chuck Palahniuk, "Haunted"


On Mon, Apr 22, 2013 at 1:32 PM, Morken, Timothy <
Timothy.Morken <@t> ucsfmedctr.org> wrote:

> Emily, It could be that the reagent is pooling off the ends of the tissue.
> Have you observed the tissue during staining to see what is happening to
> the liquid on the slide? Is this manual or automated?
>
> I agree it is strange, because the usual artifact you see with the pap pen
> is that the pen liquid covers part of the tissue and THAT part does not
> stain.
>
> Tim Morken
> Supervisor, Electron Microscopy/Neuromuscular Special Studies
> Department of Pathology
> UC San Francisco Medical Center
>
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:
> histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Emily Sours
> Sent: Monday, April 22, 2013 10:24 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] staining near pap pen only????
>
> Hello Histonetters!
>
> We have been doing triple staining on frozen tissue for a while.  Just
> recently it started to stain only near the pap pen (the sections have two
> lines of pap pen, one where they begin and one where they end).  This makes
> no sense to me, as the staining isn't only on one end (as if the tray was
> crooked) but both ends and NOT the middle.  Can anyone think of what would
> cause this?
> My boss came up with the idea that the solution is evaporating and
> therefore, it's more concentrated on the ends, but that seems a stretch.
> Then again, it's better than what I came up with, which is nothing!
>
> Emily
>
>
> "By bitching and bitching and bitching, they could exhaust the drama of
> their own horror stories. Grow bored. Only then could they accept a new
> story for their lives. Move forward."
>
> -Chuck Palahniuk, "Haunted"
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