[Histonet] RE: Calcium Stains?

[Tony Henwood (SCHN)]

Emily,

I would suggest the alizarin Red S Technique.
Since silver substitutes calcium in the Von Kossa's technique, it can therefore be regarded as a histochemical test for inorganic phosphates rather than calcium (see Meloan & Puchtler (1985) J Histotechnol 8(1):11-13). 

Alizarin Red S for Calcium allows the direct demonstration of insoluble calcium cations where Alizarin Red S forms an insoluble orange red lake.  The technique is made specific by pH control (Garvey et al 1989, J Histotechnol 12 (3):225-227). Alizarin Red S also forms lakes with other metals such as barium, aluminium, mercury and magnesium. Fortunately, the bright red colouration of calcium can be easily differentiated from the dark red of other metal-lakes.

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager & Senior Scientist 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of McKenzie, Emily
Sent: Friday, 19 April 2013 3:43 AM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] Calcium Stains?

We are having some trouble with our Von Kossa Calcium stain. Are there other stains that could be suggested to stain for Calcium in patient tissue?



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