[Histonet] RE: Pituitary gland

[Houston, Ronald]

back in the days when I worked exclusively in neuropathology in Glasgow, the tinctorial stains of choice for pituitary exams were PAS/OG and OFG (orange fuchsin green) methods.  I don't have the references to hand , although I believe the first was attributed to Tony Pearse, but can probably pull up the methods if needed


Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager
ChildLab, a Division of Nationwide Children's Hospital
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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Geoff
Sent: Thursday, April 18, 2013 10:29 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] RE: Pituitary gland

In our library all of these are still known as /Stain Technology/. The name change to /Biotechnic and Histochemistry/ happened much later.

Geoff


On 4/17/2013 9:14 PM, Tony Henwood (SCHN) wrote:
> Some references that might be useful:
>
> Paget, G. E., & Eccleston, E. (1960). Simultaneous specific demonstration of thyrotroph, gonadotroph and acidophil cells in the anterior hypophysis. Biotechnic & Histochemistry, 35(3), 119-122.
>
> Phifer, R. F., Spicer, S. S., & Hennigar, G. R. (1973). Histochemical reactivity and staining properties of functionally defined cell types in the human adenohypophysis. The American journal of pathology, 73(3), 569.
>
> Kerenyi, N. (1959). Congo red as a simple stain for beta cells of the hypophysis. Biotechnic & Histochemistry, 34(6), 343-346.
>
>
> Elftman, H. (1959). Combined aldehyde-fuchsin and periodic acid-Schiff staining of the pituitary. Biotechnic & Histochemistry, 34(2), 77-80.
>
>
>
> Read More: 
> http://informahealthcare.com/doi/abs/10.3109/10520295909114648
>
> Regards
> Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
> Laboratory Manager & Senior Scientist
> Tel: 612 9845 3306
> Fax: 612 9845 3318
> the children's hospital at westmead
> Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, 
> Westmead NSW 2145, AUSTRALIA
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu 
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of 
> Bustamante, Lin
> Sent: Thursday, 18 April 2013 1:45 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Pituitary gland
>
> Looking for any stain that shows acidophilic and basophilic cell from pituitary gland.
> Thank you.
> Lin.
>
>
> Lin S. Bustamante, B.S., H.T.(ASCP)
> VIBS Histology Laboratory Supervisor
> College Of Veterinary Medicine
> Texas A&M University
> College Station, Texas 77843-4458
> Phone: (979) 845-3177
> Fax: (979) 458-3499
>
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