[Histonet] Isopentane storage-the polling is open

[Morken, Timothy]

Paula, we keep ours at room temp in a small flam cab near the muscle bench. We keep two gallons on hand. 

 In Dapson & Dapson's book "Hazardous Materials in the Histopathology Laboratory" (Anatech, 2005) they do not make any specific recommendations concerning storage temperature, except to say IF it is kept in the refrigerator/freezer use only an explosion-proof refrigerator/freezer. 

The flash point is -50C, so a fridge is not significantly different than room temp, unless your lab is often over 30C/85F (the boiling point!). 


Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center



-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Paula Sicurello
Sent: Tuesday, April 16, 2013 10:35 AM
To: HistoNet
Subject: [Histonet] Isopentane storage-the polling is open

Hello HistoNetters,

I have been having a discussion with my boss about whether or not to store the isopentane (used for freezing muscle biopsies) in the refrigerator. (An expensive, explosion proof one that I would have to buy.)

Her only experience is with the isopentane stored in the refrigerator, my experiences are with storing it at room temperature.

What do you all do?  Room temp or refrigerated?

Any and all comments are greatly appreciated.

Thanks!

Paula

--
Paula Sicurello, HTL (ASCP)
Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710
P:  919.684.2091
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