[Histonet] Picrosirius red on cryosections

[Elizabeth Chlipala]

Laura

This type of analysis does not need to be completed on frozen sections it can be performed on routine 10% NBF fixed paraffin sections.  There are protocols on line on the website stains file.

Here is the link  http://stainsfile.info/StainsFile/stain/conektv/puchtler_psr.htm

 I would let the frozen sections air dry for at least 30 minutes and then post fix in 10% NBF for about 10 minutes and then start this procedure.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Laboratory Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
Work (303) 682-3949
Fax (303) 682-9060
Cell (303) 881-0763
liz <@t> premierlab.com
www.premierlab.com

Ship to address:

1567 Skyway Drive, Unit E
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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Laura Avogaro
Sent: Tuesday, April 16, 2013 8:30 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Picrosirius red on cryosections

Hi everyone!

I would like to stain cryosections of human atrial tissue with Picrosirius Red in order to quantify collagen fibers. Cryosections are obtained by a sample embedded in OCT compound without any fixative.
Does anyone can suggest me a protocol to perform this staining?
Thanks in advance

Laura


Laura Avogaro
Center for Biomedical Technologies (BIOTECH) University of Trento Via delle Regole, 101 38123 Mattarello (TN) - Italy
Tel:  +39 0461 283425










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