[Histonet] Re: Thioflavine Staining for Amyloid

[Bob Richmond]

Thioflavine T enjoyed a fairly brief vogue as an amyloid stain about the
time I was a pathology resident over forty of years ago. It awed the
professors of medicine because it was a fluorescent stain back when that
was a hot topic. I understood that it was similar in specificity to the old
crystal violet mounted in pancake syrup.

The standard stain for amyloid remains Congo red, viewed with polarization.
Newer direct cotton dyes like Sirius red never really caught on.

Anatech (www.anatechltdusa.com - I have no connection with Anatech)
introduced "Amyloid Red" (Direct red 72, C.I. 29200) as an amyloid stain
twelve years ago. I've yet to read about it anywhere - a Google search
turns up nothing but their catalog and a Histosearch note I wrote in 2001.
Does anyone have any experience with it?

Bob Richmond
Samurai Pathologist
Maryville TN