[Histonet] RE: Whole slide scanners.
Connolly, Brett M
brett_connolly <@t> merck.com
Thu Apr 11 14:46:02 CDT 2013
I think Scott makes a good point about having a clear need. We use the Vectra system now, and have previously used Ariol. We only scan IHC slides from projects in which we want a quantitative analysis.
Brett
Brett M. Connolly, Ph.D.
Principal Scientist, Imaging Dept.
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_connolly <@t> merck.com
T- 215-652-2501
F- 215-993-6803
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Scott A. Ely
Sent: Thursday, April 11, 2013 2:05 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Whole slide scanners.
I have worked with whole slide scanners and digital image analysis since 1998. We currently own scanners from 3 different vendors. Also, I have participated in whole slide scanner marketing research, sitting at a discussion table with a dozen people from different hospitals.
My experience has been that most departments purchase scanners without a clear need. For example, some want to scan and archive slides, to make them available for review. That makes some sense in theory. However, in every institution I've talked to, they end up paying for the scanner, a tech to do the work, the service contract and the server space to store the images, which can be huge, up to 20 *giga*bytes per *slide... then find that it is rare for anyone to ever actually look at the scanned images. Most pathologists find it is more expedient to simply pull the glass slides out of the archive. In New York, we are required by law to archive glass slides for at least 10 years anyway.
So, my advice, for what it's worth, is not to purchase any whole slide scanner unless you have a clear and well defined need (we have 3 giant systems, rarely used, taking up space and gathering dust). For example, if you need to store images from consultation slides before returning them to another institution, why not just snap a few photomicrographs? Being able to view a couple of small, representative pictures is better than having to wade through an entire scanned slide.
-------------------------------------------------------------------------------
Scott Ely, MD MPH
Associate Director, Hematopathology Fellowship Program
Section of Hematopathology
Department of Pathology
Weill Medical College of Cornell University
New York Presbyterian Hospital
Room: Starr 715
525 E. 68th Street
New York, NY 10065
PH: 212-746-2442
FAX: 212-746-2009
http://www.cornellphysicians.com/scottely/
Legal Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the original intended recipient(s) selected by Dr. Ely and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the recipient specified by Dr. Ely, please contact the sender by reply e-mail and destroy all copies of the original message.
-------------------------------------------------------------------------------
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] on behalf of histonet-request <@t> lists.utsouthwestern.edu [histonet-request <@t> lists.utsouthwestern.edu]
Sent: Thursday, April 11, 2013 1:17 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 113, Issue 10
Send Histonet mailing list submissions to
histonet <@t> lists.utsouthwestern.edu
To subscribe or unsubscribe via the World Wide Web, visit
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
or, via email, send a message with subject or body 'help' to
histonet-request <@t> lists.utsouthwestern.edu
You can reach the person managing the list at
histonet-owner <@t> lists.utsouthwestern.edu
When replying, please edit your Subject line so it is more specific
than "Re: Contents of Histonet digest..."
Today's Topics:
1. Whole slide scanners, any reviews..? (Erickson, Jamie E)
2. RE: Whole slide scanners, any reviews..? (Elizabeth Chlipala)
3. RE: Whole slide scanners, any reviews..? (Elizabeth Chlipala)
4. Want better IHC / ISH results with 10+ or even 20+ year old
FFPE specimens? (Rob Day)
5. Micro-cut H1200 Vibrotome manual (Jo-Ann Bader, Ms.)
6. Talk to me about shelf life and outdates (Cheryl)
7. RE: Talk to me about shelf life and outdates (Elizabeth Chlipala)
8. FFPE for Immunohistochemistry. (Ian R Bernard)
9. Formalin Neutralizer (Bustamante, Lin)
10. compression problems (Denise G Crowley)
11. Re: Formalin Neutralizer (Cristi Rigazio)
12. Re: Talk to me about shelf life and outdates (Emily Sours)
13. RE: compression problems (Elizabeth Chlipala)
14. RE: Talk to me about shelf life and outdates (Goins, Tresa)
15. Re: FFPE for Immunohistochemistry. (Geoff)
16. RE: FFPE for Immunohistochemistry. (Morken, Timothy)
17. Anti-human nuclear antibody (Elizabeth Cameron)
18. RE: Anti-human nuclear antibody (Elizabeth Cameron)
19. Histology Society of Ohio Symposium/Convention April 26-27
(Pam Barker)
20. HM 355S microtome users (Maria Mejia)
21. RE: Anti-human nuclear antibody (Anatoli Gleiberman)
----------------------------------------------------------------------
Message: 1
Date: Wed, 10 Apr 2013 17:17:16 +0000
From: "Erickson, Jamie E" <jamie.erickson <@t> abbvie.com>
Subject: [Histonet] Whole slide scanners, any reviews..?
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<8B946A68A8F3534A99CC493DEFB49B10131DD88D <@t> WM10002P.oneabbott.com>
Content-Type: text/plain; charset="us-ascii"
HI All,
Our Department is reviewing whole slide scanners for possible purchase soon We are looking at systems that do both Bright field and Fluorescence and I was wondering if anyone out in histoland is using the Leica (Ariol),Zeiss (axioscan Z1) or the Perkin Elmer's (Vectra) scanners. I would love to hear from you regarding your thoughts about, image quality, scanner speed, cost, image databases etc.
We hope to use this for telepathology as well as image analysis, so any thoughts would be great..
Thanks,
Jamie Erickson
Scientist II
HTL (ASCP),MS
------------------------------
Message: 2
Date: Wed, 10 Apr 2013 11:45:16 -0600
From: Elizabeth Chlipala <liz <@t> premierlab.com>
Subject: [Histonet] RE: Whole slide scanners, any reviews..?
To: "Erickson, Jamie E" <jamie.erickson <@t> abbvie.com>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<14E2C6176416974295479C64A11CB9AE016411AC2A83 <@t> SBS2K8.premierlab.local>
Content-Type: text/plain; charset="us-ascii"
Jamie
You can go to the DPA website to get a bit more information on scanners https://digitalpathologyassociation.org/ , there are some white papers up that discuss some different topics and I think the website for http://digitalpathologyconsultants.com/DPCblog/about/ Amanda works for Visopharm which is a software provider but she also has a lot of information on her site. Keith Kaplans site also has a lot of information - http://tissuepathology.com/
There are other scanners that I would considers - Phillips, Hamamatsu, etc. you should look at those too. I know that Hamamatsu also scans fluorescent but not sure about Phillips or Ventana.
Leica scanners use Slide Path for their database, I'm not sure what the others use for their database. We have an Aperio ScanScope XT but it only scans brightfield. Some companies that sell scanners also have analysis tool boxes and databases that you can purchase with the scanner and some companies only sell the scanner and you will have to figure out what you are going to do for your database, storage and analysis. If you are looking at analysis and if you are in the clinical setting I would definitely pick a company that has had some of their software 510K cleared for specific analysis (ER/PR, Her2, etc) that will help your internal validation process.
Good Luck
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Laboratory Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
Work (303) 682-3949
Fax (303) 682-9060
Cell (303) 881-0763
liz <@t> premierlab.com
www.premierlab.com
Ship to address:
1567 Skyway Drive, Unit E
Longmont, CO 80504
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Erickson, Jamie E
Sent: Wednesday, April 10, 2013 11:17 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Whole slide scanners, any reviews..?
HI All,
Our Department is reviewing whole slide scanners for possible purchase soon We are looking at systems that do both Bright field and Fluorescence and I was wondering if anyone out in histoland is using the Leica (Ariol),Zeiss (axioscan Z1) or the Perkin Elmer's (Vectra) scanners. I would love to hear from you regarding your thoughts about, image quality, scanner speed, cost, image databases etc.
We hope to use this for telepathology as well as image analysis, so any thoughts would be great..
Thanks,
Jamie Erickson
Scientist II
HTL (ASCP),MS
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 3
Date: Wed, 10 Apr 2013 11:48:42 -0600
From: Elizabeth Chlipala <liz <@t> premierlab.com>
Subject: [Histonet] RE: Whole slide scanners, any reviews..?
To: "Erickson, Jamie E" <jamie.erickson <@t> abbvie.com>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<14E2C6176416974295479C64A11CB9AE016411AC2A84 <@t> SBS2K8.premierlab.local>
Content-Type: text/plain; charset="us-ascii"
Jamie
I realized that you are coming from a pharmaceutical company - for software I would definitely look at Definians or Visiopharm. We have Definians Tissue Studio and have used some of Aperios algorithms also. Visiopharm also has some nice software too.
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Laboratory Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
Work (303) 682-3949
Fax (303) 682-9060
Cell (303) 881-0763
liz <@t> premierlab.com
www.premierlab.com
Ship to address:
1567 Skyway Drive, Unit E
Longmont, CO 80504
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Erickson, Jamie E
Sent: Wednesday, April 10, 2013 11:17 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Whole slide scanners, any reviews..?
HI All,
Our Department is reviewing whole slide scanners for possible purchase soon We are looking at systems that do both Bright field and Fluorescence and I was wondering if anyone out in histoland is using the Leica (Ariol),Zeiss (axioscan Z1) or the Perkin Elmer's (Vectra) scanners. I would love to hear from you regarding your thoughts about, image quality, scanner speed, cost, image databases etc.
We hope to use this for telepathology as well as image analysis, so any thoughts would be great..
Thanks,
Jamie Erickson
Scientist II
HTL (ASCP),MS
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 4
Date: Wed, 10 Apr 2013 15:53:41 -0400
From: Rob Day <rob <@t> foliobio.com>
Subject: [Histonet] Want better IHC / ISH results with 10+ or even 20+
year old FFPE specimens?
To: "histonet <@t> lists.utsouthwestern.edu"
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <8D62E288-3E7D-4729-B399-BE6134DAE751 <@t> foliobio.com>
Content-Type: text/plain; charset=us-ascii
Greetings histonet,
The lab where I work is developing a process to improve IHC and ISH analysis of old, archival FFPE specimens. We are looking for labs who can help us test the effectiveness of this process. If you are having difficulty getting good IHC or ISH results from 10+ or even 20+ year old specimens, let me know, and we will gladly treat some of your slides for free, if you will share the resulting images with us.
We posted this request here once before, and we did get some interest, but we are still not convinced that we have adequate independent verification.
For more information and some before and after images, go here:
http://hematoxylin-eosin-tales.blogspot.com/2013/04/new-technique-for-improving-staining-in.html
Rob Day.
------------------------------
Message: 5
Date: Wed, 10 Apr 2013 19:54:05 +0000
From: "Jo-Ann Bader, Ms." <jo-ann.bader <@t> mcgill.ca>
Subject: [Histonet] Micro-cut H1200 Vibrotome manual
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<8C36045F0065CE48906E684F15FD4CB6320B9C3E <@t> EXMBX2010-6.campus.MCGILL.CA>
Content-Type: text/plain; charset="iso-8859-1"
Hi Everyone,
I am looking for a copy of the manual for the Micro-cut Vibrotome. One of our labs was given one but do not know how to use it. The manual is no long available through Bio-Rad.
Jo-Ann Bader
Histology Co-Ordinator
Goodman Cancer Centre
McGill University 1160 Pine Ave. W - Rm 312
Montreal, QC, Canada
H3G 1Y6
Tel: 514-398-8270
------------------------------
Message: 6
Date: Wed, 10 Apr 2013 15:24:31 -0700 (PDT)
From: Cheryl <tkngflght <@t> yahoo.com>
Subject: [Histonet] Talk to me about shelf life and outdates
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<1365632671.80304.YahooMailClassic <@t> web161906.mail.bf1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
We're prepping for an inspection and I have a small amount of older powdered dyes and substances we can't get any more.??? They're dated 'Good until used'.
?
Would someone elaborate on how we came to have outdates on things that never had them before, and how those outdates are determined?
?
Is there a procedure or policy you might share so we can keep our old but good items in service until we run out rather than in 12 months (EEEK!!!)
?
Many thanks!
?
Cheryl
Cheryl Kerry, HT(ASCP)
Full Staff Inc.
Staffing the AP Lab by helping one GREAT?Tech at a time.?
281.852.9457?Office
800.756.3309?Phone & Fax?
admin <@t> fullstaff.org
Sign up for the FREE?newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews <@t> fullstaff.org. Please?include your name and specialty in the body of the email.
------------------------------
Message: 7
Date: Wed, 10 Apr 2013 16:51:08 -0600
From: Elizabeth Chlipala <liz <@t> premierlab.com>
Subject: RE: [Histonet] Talk to me about shelf life and outdates
To: Cheryl <tkngflght <@t> yahoo.com>, "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<14E2C6176416974295479C64A11CB9AE016411AC2A8B <@t> SBS2K8.premierlab.local>
Content-Type: text/plain; charset="us-ascii"
Cheryl
We are not a clinical lab, but we are GLP compliant. This is how we deal with dry chemicals and stains. A lot of times they do not come with an expiration date. If no expiration date/retest date is listed we give them an expiration date/retest date of 5 years post receipt. After that date we place them in a special area that is marked - Past Retest Date - Do not use until retested. We have a reagent control SOP that explains all of this. I'm not familiar with the "Good until used" designation. For retest we just run a special stain that contains a solution prepared from the chemical and if it stains appropriately we give that chemical a new expiration/retest date and just make sure we document the process appropriately. We just initiated this SOP recently so have not yet had to perform any retests. We update our chemical inventory as new chemicals are ordered, but once a year we review and check all our chemicals for inventory and that we have updated MSDS's available. I can send a pdf of our reagent control SOP if you would like it. If you are striving to be a GLP compliant lab and are heading to NSH convention this year I'll be giving a workshop on GLP compliance in the histology lab with our Facility Quality Assurance Manager.
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Laboratory Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
Work (303) 682-3949
Fax (303) 682-9060
Cell (303) 881-0763
liz <@t> premierlab.com
www.premierlab.com
Ship to address:
1567 Skyway Drive, Unit E
Longmont, CO 80504
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Cheryl
Sent: Wednesday, April 10, 2013 4:25 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Talk to me about shelf life and outdates
We're prepping for an inspection and I have a small amount of older powdered dyes and substances we can't get any more. They're dated 'Good until used'.
Would someone elaborate on how we came to have outdates on things that never had them before, and how those outdates are determined?
Is there a procedure or policy you might share so we can keep our old but good items in service until we run out rather than in 12 months (EEEK!!!)
Many thanks!
Cheryl
Cheryl Kerry, HT(ASCP)
Full Staff Inc.
Staffing the AP Lab by helping one GREAT Tech at a time.
281.852.9457 Office
800.756.3309 Phone & Fax
admin <@t> fullstaff.org
Sign up for the FREE newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews <@t> fullstaff.org. Please include your name and specialty in the body of the email.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 8
Date: Thu, 11 Apr 2013 10:19:16 +0000
From: Ian R Bernard <ibernard <@t> uab.edu>
Subject: [Histonet] FFPE for Immunohistochemistry.
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<D4F4C602B10B9F45B4E9271AF6380E16181ACE6A <@t> UABEXMB1.ad.uab.edu>
Content-Type: text/plain; charset="us-ascii"
Pretreatments are used to recover bonded antigen sites owing to formalin linkage. What is the optimum or maximum fixation time for tissues that may require Immunohistochemistry staining?
IB
------------------------------
Message: 9
Date: Thu, 11 Apr 2013 13:04:21 +0000
From: "Bustamante, Lin" <LBUSTAMANTE <@t> cvm.tamu.edu>
Subject: [Histonet] Formalin Neutralizer
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<94B6DC15AAF2F046BF847D4C1CA9AAC9A959FDD9 <@t> CVMMB02.cvm.tamu.edu>
Content-Type: text/plain; charset="us-ascii"
We are looking into the option of neutralizing our formalin waste instead of having it to be picked up.
If you use Formalin Neutralizer, do you have any pro/con about this product?
Thank you very much.
Lin S. Bustamante, B.S., H.T.(ASCP)
VIBS Histology Laboratory Supervisor
College Of Veterinary Medicine
Texas A&M University
College Station, Texas 77843-4458
Phone: (979) 845-3177
Fax: (979) 458-3499
------------------------------
Message: 10
Date: Thu, 11 Apr 2013 13:12:12 +0000
From: Denise G Crowley <dencrowl <@t> MIT.EDU>
Subject: [Histonet] compression problems
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<9E09CCFC753D9C4CB17FCF98945FA54E308BF1D1 <@t> OC11EXPO25.exchange.mit.edu>
Content-Type: text/plain; charset="us-ascii"
Hi all,
Has anyone experienced a problem with Paraplast Xtra over the last 3-4 months? We are going crazy with compression problems and have tried all the usual solutions (tightening all parts of microtome, changing angles, changing blades, servicing microtome) and the problem persists. This involves all 4 of our microtomes to varying degrees and all types of tissue. The only commonality is the paraffin. The tissue itself does not compress, but the paraffin around it looks like horizontal stripes. We use Paraplast Xtra for both infiltrating and embedding.
Any suggestions?
Denise Crowley
Hope Babette Tang Histology Facility
Koch Center for Integrative Cancer Research
Massachusetts Institute of Technology
500 Main St. 76-182
Cambridge MA 02139
617-258-8183
dencrowl <@t> mit.edu<mailto:dencrowl <@t> mit.edu>
------------------------------
Message: 11
Date: Thu, 11 Apr 2013 06:20:49 -0700
From: Cristi Rigazio <cls71877 <@t> gmail.com>
Subject: Re: [Histonet] Formalin Neutralizer
To: "Bustamante, Lin" <LBUSTAMANTE <@t> cvm.tamu.edu>
Cc: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <1F23ED0A-515F-4C68-8FC7-505BBF40F22C <@t> gmail.com>
Content-Type: text/plain; charset=us-ascii
We neutralize ours and have no problems with it. I am not sure how much you use, so I will say it is easiest in smaller batches as you do have to shake it up to make sure it dissolves. We purchase ours from BBC Biochemical for a very reasonable price and then test before dumping. We have doing this for four years with no issues.
Thanks,
Cristi
Sent from my iPhone
On Apr 11, 2013, at 6:04 AM, "Bustamante, Lin" <LBUSTAMANTE <@t> cvm.tamu.edu> wrote:
> We are looking into the option of neutralizing our formalin waste instead of having it to be picked up.
> If you use Formalin Neutralizer, do you have any pro/con about this product?
> Thank you very much.
>
> Lin S. Bustamante, B.S., H.T.(ASCP)
> VIBS Histology Laboratory Supervisor
> College Of Veterinary Medicine
> Texas A&M University
> College Station, Texas 77843-4458
> Phone: (979) 845-3177
> Fax: (979) 458-3499
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 12
Date: Thu, 11 Apr 2013 09:36:00 -0400
From: Emily Sours <talulahgosh <@t> gmail.com>
Subject: Re: [Histonet] Talk to me about shelf life and outdates
Cc: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<CAP=XX1ymV3Z2WwyHOW6p2C3Oyfux19XiVfqiS=wfjXNgrzA6eA <@t> mail.gmail.com>
Content-Type: text/plain; charset=UTF-8
We have stains from at least twenty years ago, judging by their
labels--they have no date on them! I always wondered how long neutral red
lasts, but apparently, it lasts a long, long time.
We are not a clincial lab though, we do research.
Emily
"By bitching and bitching and bitching, they could exhaust the drama of
their own horror stories. Grow bored. Only then could they accept a new
story for their lives. Move forward."
-Chuck Palahniuk, "Haunted"
On Wed, Apr 10, 2013 at 6:51 PM, Elizabeth Chlipala <liz <@t> premierlab.com>wrote:
> Cheryl
>
> We are not a clinical lab, but we are GLP compliant. This is how we deal
> with dry chemicals and stains. A lot of times they do not come with an
> expiration date. If no expiration date/retest date is listed we give them
> an expiration date/retest date of 5 years post receipt. After that date
> we place them in a special area that is marked - Past Retest Date - Do not
> use until retested. We have a reagent control SOP that explains all of
> this. I'm not familiar with the "Good until used" designation. For retest
> we just run a special stain that contains a solution prepared from the
> chemical and if it stains appropriately we give that chemical a new
> expiration/retest date and just make sure we document the process
> appropriately. We just initiated this SOP recently so have not yet had to
> perform any retests. We update our chemical inventory as new chemicals
> are ordered, but once a year we review and check all our chemicals for
> inventory and that we have updated MSDS's available. I can send a pdf of
> our reagent control SOP if you would like it. If you are striving to be a
> GLP compliant lab and are heading to NSH convention this year I'll be
> giving a workshop on GLP compliance in the histology lab with our Facility
> Quality Assurance Manager.
>
> Liz
>
> Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
> Laboratory Manager
> Premier Laboratory, LLC
> PO Box 18592
> Boulder, CO 80308
> Work (303) 682-3949
> Fax (303) 682-9060
> Cell (303) 881-0763
> liz <@t> premierlab.com
> www.premierlab.com
>
> Ship to address:
>
> 1567 Skyway Drive, Unit E
> Longmont, CO 80504
>
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:
> histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Cheryl
> Sent: Wednesday, April 10, 2013 4:25 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Talk to me about shelf life and outdates
>
> We're prepping for an inspection and I have a small amount of older
> powdered dyes and substances we can't get any more. They're dated 'Good
> until used'.
>
> Would someone elaborate on how we came to have outdates on things that
> never had them before, and how those outdates are determined?
>
> Is there a procedure or policy you might share so we can keep our old but
> good items in service until we run out rather than in 12 months (EEEK!!!)
>
> Many thanks!
>
> Cheryl
>
>
> Cheryl Kerry, HT(ASCP)
> Full Staff Inc.
> Staffing the AP Lab by helping one GREAT Tech at a time.
> 281.852.9457 Office
> 800.756.3309 Phone & Fax
> admin <@t> fullstaff.org
>
> Sign up for the FREE newsletter AP News--updates, tricks of the trade and
> current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe'
> request to APNews <@t> fullstaff.org. Please include your name and specialty
> in the body of the email.
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
------------------------------
Message: 13
Date: Thu, 11 Apr 2013 08:18:53 -0600
From: Elizabeth Chlipala <liz <@t> premierlab.com>
Subject: [Histonet] RE: compression problems
To: Denise G Crowley <dencrowl <@t> MIT.EDU>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<14E2C6176416974295479C64A11CB9AE016411AC2A98 <@t> SBS2K8.premierlab.local>
Content-Type: text/plain; charset="us-ascii"
Denise
We use paraplast and paraplast extra and have not experienced any problems. We just got a new lot of paraplast extra in and so far everything seems ok. I would check to see if it's a particular lot number that you are using.
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Laboratory Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
Work (303) 682-3949
Fax (303) 682-9060
Cell (303) 881-0763
liz <@t> premierlab.com
www.premierlab.com
Ship to address:
1567 Skyway Drive, Unit E
Longmont, CO 80504
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Denise G Crowley
Sent: Thursday, April 11, 2013 7:12 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] compression problems
Hi all,
Has anyone experienced a problem with Paraplast Xtra over the last 3-4 months? We are going crazy with compression problems and have tried all the usual solutions (tightening all parts of microtome, changing angles, changing blades, servicing microtome) and the problem persists. This involves all 4 of our microtomes to varying degrees and all types of tissue. The only commonality is the paraffin. The tissue itself does not compress, but the paraffin around it looks like horizontal stripes. We use Paraplast Xtra for both infiltrating and embedding.
Any suggestions?
Denise Crowley
Hope Babette Tang Histology Facility
Koch Center for Integrative Cancer Research Massachusetts Institute of Technology
500 Main St. 76-182
Cambridge MA 02139
617-258-8183
dencrowl <@t> mit.edu<mailto:dencrowl <@t> mit.edu>
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 14
Date: Thu, 11 Apr 2013 14:19:29 +0000
From: "Goins, Tresa" <TGoins <@t> mt.gov>
Subject: RE: [Histonet] Talk to me about shelf life and outdates
To: Cheryl <tkngflght <@t> yahoo.com>, "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<FA81E534F40DEF44A70F0FBBCD49D2E4E70D <@t> doaisd5235.state.mt.ads>
Content-Type: text/plain; charset="iso-8859-1"
We also have some ancient dyes in powder form that we label "Discard at Loss of Function". We were audited recently and there was no problem with the labeling, just as long as everything had a label.
Tresa
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Cheryl
Sent: Wednesday, April 10, 2013 4:25 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Talk to me about shelf life and outdates
We're prepping for an inspection and I have a small amount of older powdered dyes and substances we can't get any more.??? They're dated 'Good until used'.
?
Would someone elaborate on how we came to have outdates on things that never had them before, and how those outdates are determined?
?
Is there a procedure or policy you might share so we can keep our old but good items in service until we run out rather than in 12 months (EEEK!!!)
?
Many thanks!
?
Cheryl
Cheryl Kerry, HT(ASCP)
Full Staff Inc.
Staffing the AP Lab by helping one GREAT?Tech at a time.?
281.852.9457?Office
800.756.3309?Phone & Fax?
admin <@t> fullstaff.org
Sign up for the FREE?newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews <@t> fullstaff.org. Please?include your name and specialty in the body of the email.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 15
Date: Thu, 11 Apr 2013 10:58:04 -0400
From: Geoff <mcauliff <@t> umdnj.edu>
Subject: Re: [Histonet] FFPE for Immunohistochemistry.
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <5166CF7C.7080202 <@t> umdnj.edu>
Content-Type: text/plain; CHARSET=US-ASCII; format=flowed
It depends on the antigen. I know that sounds like a cop out but it is
true. Review the literature covering the antigen you are looking for to
see what has worked for others
Geoff
On 4/11/2013 6:19 AM, Ian R Bernard wrote:
> Pretreatments are used to recover bonded antigen sites owing to formalin linkage. What is the optimum or maximum fixation time for tissues that may require Immunohistochemistry staining?
>
> IB
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff <@t> umdnj.edu
**********************************************
------------------------------
Message: 16
Date: Thu, 11 Apr 2013 15:19:49 +0000
From: "Morken, Timothy" <Timothy.Morken <@t> ucsfmedctr.org>
Subject: [Histonet] RE: FFPE for Immunohistochemistry.
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <761E2B5697F795489C8710BCC72141FF0701B9 <@t> ex07.net.ucsf.edu>
Content-Type: text/plain; charset=us-ascii
Generally under- fixation in formalin is more of a problem than longer fixation. The reason is first that less fixation does not preserve the tissue and antigens as well (more extraction of proteins in the processing steps), and second, antigen retrieval of any type is more damaging to under-fixed tissue.
Studies have shown that 6 hours is a minimum to achieve formalin cross-linking of peptides and inactivate enzymes. After that time period antigen retrieval will be reproducible.
Longer fixation is ok. Many studies have shown good antigen retrieval for the vast majority of antigens even after months of fixation when buffer-based heat antigen retrieval is used (as opposed to enzyme digestion).
An optimum time is probably in the neighborhood of 16 to 24 hours. But most labs don't do that (clinical labs) and usually 6-12 hours fixation is used.
Her2 and ER/PR guidelines specify minimum 6 hours fixation for bx cores and trimmed tissue.
Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Ian R Bernard
Sent: Thursday, April 11, 2013 3:19 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] FFPE for Immunohistochemistry.
Pretreatments are used to recover bonded antigen sites owing to formalin linkage. What is the optimum or maximum fixation time for tissues that may require Immunohistochemistry staining?
IB
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 17
Date: Thu, 11 Apr 2013 15:21:22 +0000
From: Elizabeth Cameron <Elizabeth.Cameron <@t> jax.org>
Subject: [Histonet] Anti-human nuclear antibody
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<BE1BF4334E17294E9ABFBB2FF93398E1449CF97F <@t> jaxbhexms01.jax.org>
Content-Type: text/plain; charset="us-ascii"
Just wondering if anyone has had any luck with Millipore anti-nuclear antibody MAB1281, and if so, what was your protocol? We are trying to detect human cells in mice.
Thank you.
Elizabeth M. Cameron, HT, QIHC (ASCP)
Histology Supervisor
The Jackson Laboratory
Bar Harbor, Maine
The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as possible.
------------------------------
Message: 18
Date: Thu, 11 Apr 2013 15:22:55 +0000
From: Elizabeth Cameron <Elizabeth.Cameron <@t> jax.org>
Subject: [Histonet] RE: Anti-human nuclear antibody
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<BE1BF4334E17294E9ABFBB2FF93398E1449CF997 <@t> jaxbhexms01.jax.org>
Content-Type: text/plain; charset="us-ascii"
Forgot to mention this is on FFPE tissue....
From: Elizabeth Cameron
Sent: Thursday, April 11, 2013 11:21 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Anti-human nuclear antibody
Just wondering if anyone has had any luck with Millipore anti-nuclear antibody MAB1281, and if so, what was your protocol? We are trying to detect human cells in mice.
Thank you.
Elizabeth M. Cameron, HT, QIHC (ASCP)
Histology Supervisor
The Jackson Laboratory
Bar Harbor, Maine
The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as possible.
------------------------------
Message: 19
Date: Thu, 11 Apr 2013 11:41:07 -0400
From: "Pam Barker" <relia1 <@t> earthlink.net>
Subject: [Histonet] Histology Society of Ohio Symposium/Convention
April 26-27
To: "Histonet" <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <00d201ce36ca$fdeacbc0$f9c06340$@earthlink.net>
Content-Type: text/plain; charset="us-ascii"
Hi Histonetters!!
I hope everyone is having a great day. I was asked by a friend to forward
this information to you about the Histology Society of Ohio's
Symposium/Convention which will be held April 26-27 2013 at the Holiday Inn
Cleveland- West
For more info and the program schedule please go to www.ohiohistology.org
Histology Society of Ohio
Symposium/Convention
April 26-27, 2013
Holiday Inn Cleveland-West
Westlake, OH
www.ohiohistology.org
Thanks-Pam
Right Place, Right Time, Right Move with RELIA!
Thank You!
Pam M. Barker
Pam Barker
President/Senior Recruiting Specialist-Histology
RELIA Solutions
Specialists in Allied Healthcare Recruiting
5703 Red Bug Lake Road #330
Winter Springs, FL 32708-4969
Phone: (407)657-2027
Cell: (407)353-5070
FAX: (407)678-2788
E-mail: relia1 <@t> earthlink.net
www.facebook.com <http://www.facebook.com/PamBarkerRELIA> /PamBarkerRELIA
www.linkedin.com/in/reliasolutions
www.twitter.com/pamatrelia
------------------------------
Message: 20
Date: Thu, 11 Apr 2013 09:10:17 -0700
From: Maria Mejia <mbmphoto <@t> gmail.com>
Subject: [Histonet] HM 355S microtome users
To: "Histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <D592545D-FE0E-4FF0-8A4C-23B1898E0948 <@t> gmail.com>
Content-Type: text/plain; charset=us-ascii
Is anyone currently using the HM 355S full automatic rotary microtome? Please can anyone
give me a review of this particular microtome - pros & cons would be very helpful from those
individuals actually using this machine on a daily basis.
My lab is considering purchasing this microtome. I would be most grateful for any assistance
users can provide.
Best
Maria Mejia
Affymetrix, Inc
------------------------------
Message: 21
Date: Thu, 11 Apr 2013 16:43:05 +0000
From: Anatoli Gleiberman <AGleiberman <@t> cbiolabs.com>
Subject: [Histonet] RE: Anti-human nuclear antibody
To: Elizabeth Cameron <Elizabeth.Cameron <@t> jax.org>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<77BC2EEB6AC66C49AEF794DC98BE314C016DC952F3 <@t> cbiolabs05.CBiolabs.local>
Content-Type: text/plain; charset="us-ascii"
Elizabeth,
We are able to detect human cells in mice organs using MAB1273 anti-mitochondria antibody (1:50) from Millipore on FFPE sections after standard HIER retrieval. As a detection reagent we use MaxFluor 594 Mouse on Mouse IF detection kit (MaxVision, cat.No MF03),
Anatoli Gleiberman, PhD
Director of Histopathology
Cleveland Biolabs, Inc
73 High Street
Buffalo, NY 14203
phone:716-849-6810 ext.354
fax:716-849-6817
e-mail: AGleiberman <@t> cbiolabs.com
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Elizabeth Cameron
Sent: Thursday, April 11, 2013 11:21 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Anti-human nuclear antibody
Just wondering if anyone has had any luck with Millipore anti-nuclear antibody MAB1281, and if so, what was your protocol? We are trying to detect human cells in mice.
Thank you.
Elizabeth M. Cameron, HT, QIHC (ASCP)
Histology Supervisor
The Jackson Laboratory
Bar Harbor, Maine
The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as possible.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information.
------------------------------
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
End of Histonet Digest, Vol 113, Issue 10
*****************************************
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Notice: This e-mail message, together with any attachments, contains
information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from
your system.
More information about the Histonet
mailing list